Diyala Agricultural Sciences Journal, 12 (2): 33-39, 2020 8746-eISSN: 2310 9524,-ISSN: 2073 ــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ 33 https://journal.djas.uodiyala.edu.iq/index.php/dasj/issue/archive NEW RECORD FOR TWO SPECIES OF Psallopsis reuter, 1901 (HEMIPTERA: HETEROPTERA: MIRIDAE) FROM IRAQ Hussein Ali Mutney Al-Anbaki 1 Iman Mohammed Al-Malo 2 1 Dept of Soil Sciences and Water Resources, College of Agriculture, University of Diyala. Iraq. 2 Dept of Plant Protection, College of Agricultural Engineering Sciences, Univ. of Baghdad, Iraq. 1 Corresponding author: humamalanbaki@gmail.com ABSTRACT New record of two species of genus Psallopsis Reuter, 1901 belongs to Subfamily phylinae and to the Family Miridae and to the suborder of Heteroptera of order Hemiptera, these species as Psallopsis minimus (Wagner, 1967) and P. kalidiicola Konstantinov, 1997, which collected 23 twenty three insects from Baghdad / Al- Husayniya and Abu-Gharib, the other collected 20 twenty insects from Baghdad / Taji and Abu-Gharib and Al- Rashdiya, this species are a new record in Iraq during the study at 2018 – 2019. The insects Indented by used taxonomic keys depended on morphological characters and male genitalia. Used camera Lucida to draw bodies' part and picture by Dino- Lite microscope camera. Key word: Psallopsis., phylinae, Miridae, Hemiptera, Iraq. Psallopsis reuter, 1901تسجيل جديد لنوعيه مه بك النبات التابع للجنس (HEMIPTERA:HETEROPTERA: MIRIDAE) في العراق حسيه علي مطني 1 ايمان دمحم المالو 2 1 انؼشاق.جايؼت دَانً ، ،كهُت انزساػت ،ى ػهىو انخشبت وانًىاسد انًائُتلس 2 ، انؼشاق .جايؼت بغذاد ،ػهىو انهنذست انزساػُتكهُت ،اثلسى ولاَت اننب 1 humamalanbaki@gmail.com انًشاسم:انباحث المستخلص وانزٌ َخبغ Psallopsis Reuter, 1901حسجُم جذَذ ننىػُن ين حششاث انبك وانخابؼت نهجنس سحبت - Heteropteraسحُبت يخخهفت االجنحت - Miridaeضًن ػائهت بك اننباث phylinaeػىَهت .Pوانثانٍ Psallopsis minimus (Wagner, 1967)، اننىع االول Hemipteraنصفُت االجنحت kalidiicola Konstantinov, 1997 حششة نهنىع االول ين يناطك يحافظت بغذاد 23، حى جًغ حششة ين يحافظت بغذاد )انخاجٍ ، ابى غشَب 22وجًؼج نهنىع انثانٍ )انحسُنُت وابى غشَب( ، شخصج انحششاث وفك انًفاحُح انخصنُفُت ورنك باالػخًاد 2212 – 2212وانشاشذَت( خالل يىسى ، اسخخذيج انكايُشا male genitaliaػهً صفاث انشكم انخاسجٍ واالػضاء انخكاثشَت انزكشَت . جزاء االخشي بكايُشة يجهش انذَنىجسى انًخخهفت وصىسث االانهىسُذا نشسى اجزاء ان ، نصفُت االجنحت ، انؼشاق . Psallopsis ،phylinae ،Miridaeالكلمات المفتاحية : mailto:humamalanbaki@gmail.com mailto:humamalanbaki@gmail.com Diyala Agricultural Sciences Journal, 12 (2): 33-39, 2020 8746-eISSN: 2310 9524,-ISSN: 2073 ــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ 33 https://journal.djas.uodiyala.edu.iq/index.php/dasj/issue/archive INTRODUCTION Miridae (Plant bugs) are the largest family of Heteroptera and the members exhibit wide diversity in morphology, biology and host associations, The world fauna of Miridae at present contains about 1300 genera with approximately 11,000 described species comprising about 25% of all described species of Heteroptera (Cassis and Schuh, 2012). The majority of plant bugs are herbivorous and often closely associated with particular host plants (Wheeler, 2001). Subfamily Phylinae, being the second largest subfamily of plant bugs, remains a taxonomically challenging group with many genera lacking adequate diagnoses. The subfamily is especially species-rich in the Mediterranean ecosystems, steppes, shrublands, and deserts. In the Palearctic Region phylines are represented by more than 1322 described species (Kerzhner and Josifov, 1999) and many more remain undescribed. The first of described this genus Reuter (1901), this genus can be characterized as lengths not exceeding 4 mm, protrusion tylus, macroptera, male reproductive organs Characterized if the right side piece is right spoon shaped (Konstantinov, 1997). the Species Psallopsis minimus distribution in Central and Eastern Kazakhstan, Mongolia and North China (Qi and Nonnaizab, 1996). P. kalidiicola distribution in Turkmenistan, Kazakhstan, Kirgizia, Mongolia. (Konstantinov, 1997). MATERIALS AND METHODS Adult insects were collected by using light traps (220 volts, 20 watt Black light UVB tubes in Baghdad province / Al- Husayniya, Abu-Gharib / Taji and Al- Rashdiya Samples were transferred to lab by plastic case by used smooth brush. date of collection were recorded. Insects Indented by used taxonomic keys, depending on morphological characters and described as in (Qi and Noonaizab, 1996; Konstantinov , 1997) , Use a Dino- Lite microscope camera to photograph insects, drawing the male genitalia parts by camera Lucida, the measurements of the body were taken by role, as well as in the digital image analysis program (Image J,) (Alsaad & Albahidly, 2018) to compared it in both method . Preparation of Male genitalia Male genitalia were dissected using the technique described by (Kelton ,1959; Kerzhner and Konstantinov, 1999). the specimens were used for the study of genitalia. The male specimen was gently supported on a cork piece on its back and with the help of a fine needle the abdomen was detached from the thorax at the junction of the two The abdomen was then transferred to a test tube containing a little milliliters of 10% KOH. This was heated slowly in a water Diyala Agricultural Sciences Journal, 12 (2): 33-39, 2020 8746-eISSN: 2310 9524,-ISSN: 2073 ــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ 33 https://journal.djas.uodiyala.edu.iq/index.php/dasj/issue/archive bath till the convection currents were observed in the solution. The abdomen was transferred to a glass cavity dish containing water and the macerated soft tissues were pressed out with the help of a pair of bent needles. After repeated washings in water, the abdomen was transferred to glycerine in a glass cavity dish for further dissection (separation of genital parts from the genital capsule) and observation under a Compound microscope with camera Lucida. RESULTS AND DISCUSSION Taxonomic status Order : Hemiptera Suborder : Heteroptera Superfamily : Miroidea Family : Miridae Subfamily : phylinae Genus : Psallopsis Genus : Psallopsis Reuter, 1901 Green - yellowish color, body covered with white silvery setae, medium in size less than 4 mm, Macropterous, elongate or oval species, male genitalia with Right paramere spoon shaped. 1- Species Psallopsis minimus (Wagner, 1967) Synonyme: Malthucosoma minima Wagner, 1967 ; Psallopsis minimus Kerzhner, 1970; Solenoxyphus viridulus Qi & Nonnaizab, 1996 . Adult: body length 2.9-3 mm, body, green- yellowish in color, (Fig. 1- A). Head: 0.42-0.44 mm in length. Greenish yellow covered with long white pale setae 0.15 mm length, eyes black, (fig. 1-B). Rostrum extending to second abdominal segment 1.15 mm is long. Antenna: 2.2- 2.27 mm in length, the first is 0.24-0.25mm shorter than the second 1-1.02mm, the third is 0.58-0.6mm, fourth is 0.38-0.4mm, All antennal segments pale. Diyala Agricultural Sciences Journal, 12 (2): 33-39, 2020 8746-eISSN: 2310 9524,-ISSN: 2073 ــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ 33 https://journal.djas.uodiyala.edu.iq/index.php/dasj/issue/archive Thorax: pronotum 0. 41- 0. 43 mm In length, without distinct collar, greenish yellow with long semierect setae, lateral margins linear, rounded, posterior margin medially concave, mesoscutum yellow pale, flat, scutellum greenish yellow color, (fig. 1-B). The legs: white- yellowish in color with long white pale setae and claws brown or black. The wings: Hemelytra is 2.54-2.57 mm length, white pale and Fuscous spots on it; wing membrane with two cell and fuscous spots on it, Oblique fuscous macula at base of membrane, (fig.1-A). Abdomen: is 0.95- 0.97 mm in width, pale green - greenish yellow color, the Pygophore is conical or triangular with the protruding phalotheca, (Fig.1-C). Male genitalia: - Right paramere small, (Fig.1-D); Left paramere small, boat shaped, with posterior process finger-like (Fig.1-E); Endosome S-shaped, large, (Fig.1-F); phallotheca L-shaped with apex tapering, (Fig.1-G). Distribution : Central and Eastern Kazakhstan, Mongolia and North China (Qi and Nonnaizab, 1996). Material exam : 23 adult is Collected from Baghdad province in 9/9/2019 and 19/9/2019 in Al- Husayniya and Abu-Gharib Respectively . 2- Species: Psallopsis kalidiicola Konstantinov, 1997 Adult: body length 3.1-3.4 mm, green- greenish pale in color, (Fig. 2- A). Head: 0.3-0.33 mm in length , nearly parallel - sided. greenish yellow covered with long white pale setae 0.13 mm length, eyes projecting black, (fig. 2-B). Rostrum extending to third abdominal segment 1.11 mm is long. Antenna: 1.61- 1.61 mm in length, the first is 0.22-0.24 mm shorter than the second 0.87- 0.92 mm, the third is 0.0.34-0.34 mm, fourth is 0.18-0.2 mm, All antennal segments pale. Thorax: pronotum 0. 47- 0. 5 mm In length, without distinct collar, greenish yellow with long semierect setae, lateral margins linear, rounded, posterior margin medially concave, mesoscutum greenish yellow pale, flat, scutellum greenish pale color, (fig. 2-B). The legs: white- yellowish in color with long white pale setae and and claws brown or black. Diyala Agricultural Sciences Journal, 12 (2): 33-39, 2020 8746-eISSN: 2310 9524,-ISSN: 2073 ــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ 33 https://journal.djas.uodiyala.edu.iq/index.php/dasj/issue/archive The wings: Hemelytra is 2.75-2.85 mm length, white pale and density Fuscous spots on it; wing membrane with two cell and fuscous spots on it, Density of spots on membrane, (fig.2-A). Abdomen: is 0.8-0.9 mm in width, pale green - greenish yellow color, the Pygophore is conical or triangular, (Fig.2-C). Male genitalia: - Right paramere small, (Fig.2-D); Left paramere large, boat shaped, (Fig.2-E); Endosome S-shaped, large, (Fig.2-F); phallotheca curved triangle -shaped with apex tapering, (Fig.2-G). Distribution: Turkmenistan, Kazakhstan, Kirgizia, Mongolia. (Konstantinov, 1997). Iran (Linnavuori and Modarres, 1999). Material exam : 20 adult is Collected from Baghdad province - Taji in 4/4/2019, Abu-Gharib in 13/5/2019 and Al- Rashdiya in 16/9/2019. Fig.1 Psallopsis minimus A- Adult insect (50X); B- Head and pronotum (100X); C- Pygophore (100X) 1- phallotheca; D- Right paramere; E- Left paramere; F- Endosome; G- phallotheca. Diyala Agricultural Sciences Journal, 12 (2): 33-39, 2020 8746-eISSN: 2310 9524,-ISSN: 2073 ــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ 33 https://journal.djas.uodiyala.edu.iq/index.php/dasj/issue/archive Fig.2 Psallopsis kalidiicola A- Adult insect (50X); B- Head and pronotum (100X); C- Pygophore (100X) 1- phallotheca; D- Right paramere; E- Left paramere; F- Endosome; G- phalotheca REFERENCES Al-said, L.A. and Al-Bahadli H.H. 2018. 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Diyala Agricultural Sciences Journal, 12 (2): 33-39, 2020 8746-eISSN: 2310 9524,-ISSN: 2073 ــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ 33 https://journal.djas.uodiyala.edu.iq/index.php/dasj/issue/archive Kerzhner, I. M. and M. Josifov. 1999. Catalogue of the Heteroptera of the Palearctic Region, Cimicomorpha II. B. Aukema and C. Rieger (eds.). Neth. Entomol. Soc., 3: 577 pp. Konstantinov, F. V. 1997. A revision of the genus Psallopsis (Heteroptera: Miridae). Zoosystematica Rossica, 6(1/2): 171-190. Linnavuori, R. E. and Modarres, M. 1999. Studies on the Heteroptera of the Khorasan province in N.E. Iran. II. Cimicomorpha: Miridae.- Entomol. Fennica 10: 215-231. Qi, B, and Nonnaizab. 1996. New and little-known species of genus Solenoxyphus Reuter from northern china (Hemiptera: Miridae). Entom. Sinica, 3(4): 293-299. Wagner, E. 1967. 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