id	sid	tid	token	lemma	pos
ajst-25583	1	1	academic	academic	ADJ
ajst-25583	1	2	journal	journal	NOUN
ajst-25583	1	3	of	of	ADP
ajst-25583	1	4	science	science	NOUN
ajst-25583	1	5	and	and	CCONJ
ajst-25583	1	6	technology	technology	NOUN
ajst-25583	1	7	issn	issn	NOUN
ajst-25583	1	8	:	:	PUNCT
ajst-25583	1	9	2771	2771	NUM
ajst-25583	1	10	-	-	SYM
ajst-25583	1	11	3032	3032	NUM
ajst-25583	1	12	|	|	NOUN
ajst-25583	1	13	vol	vol	NOUN
ajst-25583	1	14	.	.	PROPN
ajst-25583	2	1	12	12	NUM
ajst-25583	2	2	,	,	PUNCT
ajst-25583	2	3	no	no	INTJ
ajst-25583	2	4	.	.	NOUN
ajst-25583	2	5	2	2	NUM
ajst-25583	2	6	,	,	PUNCT
ajst-25583	2	7	2024	2024	NUM
ajst-25583	2	8	102	102	NUM
ajst-25583	2	9	research	research	NOUN
ajst-25583	2	10	progress	progress	NOUN
ajst-25583	2	11	of	of	ADP
ajst-25583	2	12	crispr	crispr	ADJ
ajst-25583	2	13	‐based	‐base	VERB
ajst-25583	2	14	bacillus	bacillus	NOUN
ajst-25583	2	15	subtilis	subtili	NOUN
ajst-25583	2	16	system	system	NOUN
ajst-25583	2	17	siyu	siyu	PROPN
ajst-25583	2	18	liu	liu	PROPN
ajst-25583	2	19	,	,	PUNCT
ajst-25583	2	20	wenke	wenke	NOUN
ajst-25583	2	21	zhou	zhou	PROPN
ajst-25583	2	22	*	*	PUNCT
ajst-25583	2	23	school	school	NOUN
ajst-25583	2	24	of	of	ADP
ajst-25583	2	25	environmental	environmental	ADJ
ajst-25583	2	26	ecology	ecology	NOUN
ajst-25583	2	27	and	and	CCONJ
ajst-25583	2	28	biological	biological	ADJ
ajst-25583	2	29	engineering	engineering	NOUN
ajst-25583	2	30	,	,	PUNCT
ajst-25583	2	31	wuhan	wuhan	PROPN
ajst-25583	2	32	institute	institute	PROPN
ajst-25583	2	33	of	of	ADP
ajst-25583	2	34	technology	technology	PROPN
ajst-25583	2	35	university	university	PROPN
ajst-25583	2	36	,	,	PUNCT
ajst-25583	2	37	wuhan	wuhan	PROPN
ajst-25583	2	38	430200	430200	NUM
ajst-25583	2	39	,	,	PUNCT
ajst-25583	2	40	china	china	PROPN
ajst-25583	2	41	*	*	PUNCT
ajst-25583	2	42	corresponding	correspond	VERB
ajst-25583	2	43	author	author	NOUN
ajst-25583	2	44	:	:	PUNCT
ajst-25583	3	1	wenke	wenke	PROPN
ajst-25583	3	2	zhou	zhou	PROPN
ajst-25583	3	3	abstract	abstract	PROPN
ajst-25583	3	4	:	:	PUNCT
ajst-25583	3	5	as	as	ADP
ajst-25583	3	6	a	a	DET
ajst-25583	3	7	typical	typical	ADJ
ajst-25583	3	8	industrial	industrial	ADJ
ajst-25583	3	9	model	model	NOUN
ajst-25583	3	10	of	of	ADP
ajst-25583	3	11	food	food	NOUN
ajst-25583	3	12	safety	safety	NOUN
ajst-25583	3	13	,	,	PUNCT
ajst-25583	3	14	the	the	DET
ajst-25583	3	15	bacillus	bacillus	NOUN
ajst-25583	3	16	subtilis	subtilis	NOUN
ajst-25583	3	17	has	have	VERB
ajst-25583	3	18	the	the	DET
ajst-25583	3	19	characteristics	characteristic	NOUN
ajst-25583	3	20	of	of	ADP
ajst-25583	3	21	non	non	ADJ
ajst-25583	3	22	-pathogenicity	-pathogenicity	NOUN
ajst-25583	3	23	,	,	PUNCT
ajst-25583	3	24	strong	strong	ADJ
ajst-25583	3	25	extracellular	extracellular	ADJ
ajst-25583	3	26	secretion	secretion	NOUN
ajst-25583	3	27	protein	protein	NOUN
ajst-25583	3	28	ability	ability	NOUN
ajst-25583	3	29	,	,	PUNCT
ajst-25583	3	30	and	and	CCONJ
ajst-25583	3	31	no	no	DET
ajst-25583	3	32	obvious	obvious	ADJ
ajst-25583	3	33	password	password	NOUN
ajst-25583	3	34	preferences	preference	NOUN
ajst-25583	3	35	,	,	PUNCT
ajst-25583	3	36	which	which	PRON
ajst-25583	3	37	have	have	AUX
ajst-25583	3	38	been	be	AUX
ajst-25583	3	39	widely	widely	ADV
ajst-25583	3	40	used	use	VERB
ajst-25583	3	41	in	in	ADP
ajst-25583	3	42	the	the	DET
ajst-25583	3	43	field	field	NOUN
ajst-25583	3	44	of	of	ADP
ajst-25583	3	45	metabolic	metabolic	NOUN
ajst-25583	3	46	engineering	engineering	NOUN
ajst-25583	3	47	.	.	PUNCT
ajst-25583	4	1	in	in	ADP
ajst-25583	4	2	recent	recent	ADJ
ajst-25583	4	3	years	year	NOUN
ajst-25583	4	4	,	,	PUNCT
ajst-25583	4	5	with	with	ADP
ajst-25583	4	6	the	the	DET
ajst-25583	4	7	rapid	rapid	ADJ
ajst-25583	4	8	development	development	NOUN
ajst-25583	4	9	of	of	ADP
ajst-25583	4	10	molecular	molecular	ADJ
ajst-25583	4	11	biology	biology	NOUN
ajst-25583	4	12	and	and	CCONJ
ajst-25583	4	13	genetic	genetic	ADJ
ajst-25583	4	14	engineering	engineering	NOUN
ajst-25583	4	15	technology	technology	NOUN
ajst-25583	4	16	,	,	PUNCT
ajst-25583	4	17	various	various	ADJ
ajst-25583	4	18	technologies	technology	NOUN
ajst-25583	4	19	have	have	AUX
ajst-25583	4	20	been	be	AUX
ajst-25583	4	21	applied	apply	VERB
ajst-25583	4	22	to	to	ADP
ajst-25583	4	23	bacillus	bacillus	NOUN
ajst-25583	4	24	subtilis	subtili	NOUN
ajst-25583	4	25	,	,	PUNCT
ajst-25583	4	26	thereby	thereby	ADV
ajst-25583	4	27	synthesizing	synthesize	VERB
ajst-25583	4	28	biological	biological	ADJ
ajst-25583	4	29	products	product	NOUN
ajst-25583	4	30	.	.	PUNCT
ajst-25583	5	1	this	this	DET
ajst-25583	5	2	article	article	NOUN
ajst-25583	5	3	detailed	detail	VERB
ajst-25583	5	4	the	the	DET
ajst-25583	5	5	application	application	NOUN
ajst-25583	5	6	of	of	ADP
ajst-25583	5	7	crispr	crispr	NOUN
ajst-25583	5	8	and	and	CCONJ
ajst-25583	5	9	crispr	crispr	ADJ
ajst-25583	5	10	on	on	ADP
ajst-25583	5	11	bacillus	bacillus	NOUN
ajst-25583	5	12	subtilis	subtili	NOUN
ajst-25583	5	13	,	,	PUNCT
ajst-25583	5	14	and	and	CCONJ
ajst-25583	5	15	summarized	summarize	VERB
ajst-25583	5	16	the	the	DET
ajst-25583	5	17	biological	biological	ADJ
ajst-25583	5	18	products	product	NOUN
ajst-25583	5	19	of	of	ADP
ajst-25583	5	20	bacillus	bacillus	NOUN
ajst-25583	5	21	subtilis	subtili	NOUN
ajst-25583	5	22	in	in	ADP
ajst-25583	5	23	related	relate	VERB
ajst-25583	5	24	fields	field	NOUN
ajst-25583	5	25	and	and	CCONJ
ajst-25583	5	26	the	the	DET
ajst-25583	5	27	outlook	outlook	NOUN
ajst-25583	5	28	on	on	ADP
ajst-25583	5	29	its	its	PRON
ajst-25583	5	30	future	future	ADJ
ajst-25583	5	31	research	research	NOUN
ajst-25583	5	32	direction	direction	NOUN
ajst-25583	5	33	.	.	PUNCT
ajst-25583	6	1	keywords	keyword	NOUN
ajst-25583	6	2	:	:	PUNCT
ajst-25583	6	3	bacillus	bacillus	NOUN
ajst-25583	6	4	subtilis	subtili	NOUN
ajst-25583	6	5	;	;	PUNCT
ajst-25583	6	6	crispr	crispr	ADJ
ajst-25583	6	7	;	;	PUNCT
ajst-25583	6	8	metabolic	metabolic	NOUN
ajst-25583	6	9	engineering	engineering	NOUN
ajst-25583	6	10	.	.	PUNCT
ajst-25583	7	1	1	1	X
ajst-25583	7	2	.	.	X
ajst-25583	7	3	bacillus	bacillus	NOUN
ajst-25583	7	4	subtilis	subtili	NOUN
ajst-25583	7	5	1.1	1.1	NUM
ajst-25583	7	6	.	.	PUNCT
ajst-25583	8	1	introduction	introduction	NOUN
ajst-25583	8	2	to	to	ADP
ajst-25583	8	3	bacillus	bacillus	NOUN
ajst-25583	8	4	subtilis	subtili	NOUN
ajst-25583	8	5	bacillus	bacillus	NOUN
ajst-25583	8	6	subtilis	subtili	NOUN
ajst-25583	8	7	.	.	PUNCT
ajst-25583	9	1	it	it	PRON
ajst-25583	9	2	is	be	AUX
ajst-25583	9	3	a	a	DET
ajst-25583	9	4	gram	gram	NOUN
ajst-25583	9	5	-	-	PUNCT
ajst-25583	9	6	positive	positive	ADJ
ajst-25583	9	7	bacterium	bacterium	NOUN
ajst-25583	9	8	,	,	PUNCT
ajst-25583	9	9	which	which	PRON
ajst-25583	9	10	has	have	VERB
ajst-25583	9	11	flagella	flagella	NOUN
ajst-25583	9	12	on	on	ADP
ajst-25583	9	13	its	its	PRON
ajst-25583	9	14	surface	surface	NOUN
ajst-25583	9	15	and	and	CCONJ
ajst-25583	9	16	endospore	endospore	NOUN
ajst-25583	9	17	formation	formation	NOUN
ajst-25583	9	18	in	in	ADP
ajst-25583	9	19	its	its	PRON
ajst-25583	9	20	body	body	NOUN
ajst-25583	9	21	that	that	PRON
ajst-25583	9	22	can	can	AUX
ajst-25583	9	23	survive	survive	VERB
ajst-25583	9	24	against	against	ADP
ajst-25583	9	25	harsh	harsh	ADJ
ajst-25583	9	26	external	external	ADJ
ajst-25583	9	27	environments	environment	NOUN
ajst-25583	9	28	.	.	PUNCT
ajst-25583	10	1	in	in	ADP
ajst-25583	10	2	addition	addition	NOUN
ajst-25583	10	3	,	,	PUNCT
ajst-25583	10	4	bacillus	bacillus	NOUN
ajst-25583	10	5	subtilis	subtili	NOUN
ajst-25583	10	6	is	be	AUX
ajst-25583	10	7	attractive	attractive	ADJ
ajst-25583	10	8	because	because	SCONJ
ajst-25583	10	9	of	of	ADP
ajst-25583	10	10	its	its	PRON
ajst-25583	10	11	broad	broad	ADJ
ajst-25583	10	12	substrate	substrate	NOUN
ajst-25583	10	13	range	range	NOUN
ajst-25583	10	14	and	and	CCONJ
ajst-25583	10	15	its	its	PRON
ajst-25583	10	16	ability	ability	NOUN
ajst-25583	10	17	to	to	PART
ajst-25583	10	18	survive	survive	VERB
ajst-25583	10	19	harsh	harsh	ADJ
ajst-25583	10	20	industrial	industrial	ADJ
ajst-25583	10	21	fermentation	fermentation	NOUN
ajst-25583	10	22	conditions	condition	NOUN
ajst-25583	10	23	.	.	PUNCT
ajst-25583	11	1	it	it	PRON
ajst-25583	11	2	digests	digest	VERB
ajst-25583	11	3	lignocellulosic	lignocellulosic	ADJ
ajst-25583	11	4	materials	material	NOUN
ajst-25583	11	5	,	,	PUNCT
ajst-25583	11	6	produces	produce	VERB
ajst-25583	11	7	a	a	DET
ajst-25583	11	8	wide	wide	ADJ
ajst-25583	11	9	range	range	NOUN
ajst-25583	11	10	of	of	ADP
ajst-25583	11	11	industrial	industrial	ADJ
ajst-25583	11	12	enzymes	enzyme	NOUN
ajst-25583	11	13	,	,	PUNCT
ajst-25583	11	14	and	and	CCONJ
ajst-25583	11	15	has	have	VERB
ajst-25583	11	16	excellent	excellent	ADJ
ajst-25583	11	17	secretion	secretion	NOUN
ajst-25583	11	18	capabilities	capability	NOUN
ajst-25583	11	19	,	,	PUNCT
ajst-25583	11	20	thus	thus	ADV
ajst-25583	11	21	reducing	reduce	VERB
ajst-25583	11	22	the	the	DET
ajst-25583	11	23	cost	cost	NOUN
ajst-25583	11	24	of	of	ADP
ajst-25583	11	25	biomass	biomass	NOUN
ajst-25583	11	26	pretreatment	pretreatment	NOUN
ajst-25583	11	27	and	and	CCONJ
ajst-25583	11	28	minimizing	minimize	VERB
ajst-25583	11	29	the	the	DET
ajst-25583	11	30	need	need	NOUN
ajst-25583	11	31	for	for	ADP
ajst-25583	11	32	downstream	downstream	ADJ
ajst-25583	11	33	processing	processing	NOUN
ajst-25583	11	34	[	[	X
ajst-25583	11	35	1	1	NUM
ajst-25583	11	36	,	,	PUNCT
ajst-25583	11	37	2	2	NUM
ajst-25583	11	38	]	]	PUNCT
ajst-25583	11	39	.	.	PUNCT
ajst-25583	12	1	until	until	ADP
ajst-25583	12	2	recently	recently	ADV
ajst-25583	12	3	,	,	PUNCT
ajst-25583	12	4	bacillus	bacillus	NOUN
ajst-25583	12	5	subtilis	subtili	NOUN
ajst-25583	12	6	has	have	AUX
ajst-25583	12	7	been	be	AUX
ajst-25583	12	8	shown	show	VERB
ajst-25583	12	9	to	to	PART
ajst-25583	12	10	be	be	AUX
ajst-25583	12	11	a	a	DET
ajst-25583	12	12	potentially	potentially	ADV
ajst-25583	12	13	excellent	excellent	ADJ
ajst-25583	12	14	chassis	chassis	NOUN
ajst-25583	12	15	host	host	NOUN
ajst-25583	12	16	for	for	ADP
ajst-25583	12	17	the	the	DET
ajst-25583	12	18	production	production	NOUN
ajst-25583	12	19	of	of	ADP
ajst-25583	12	20	highvalue	highvalue	NOUN
ajst-25583	12	21	chemicals	chemical	NOUN
ajst-25583	12	22	and	and	CCONJ
ajst-25583	12	23	pharmaceuticals	pharmaceutical	NOUN
ajst-25583	12	24	(	(	PUNCT
ajst-25583	12	25	e.g.	e.g.	ADV
ajst-25583	12	26	,	,	PUNCT
ajst-25583	12	27	shark	shark	NOUN
ajst-25583	12	28	inositol	inositol	NOUN
ajst-25583	12	29	,	,	PUNCT
ajst-25583	12	30	etc	etc	X
ajst-25583	12	31	.	.	X
ajst-25583	12	32	)	)	PUNCT
ajst-25583	13	1	[	[	X
ajst-25583	13	2	3	3	NUM
ajst-25583	13	3	,	,	PUNCT
ajst-25583	13	4	4	4	NUM
ajst-25583	13	5	]	]	PUNCT
ajst-25583	13	6	.	.	PUNCT
ajst-25583	14	1	bacillus	bacillus	NOUN
ajst-25583	14	2	subtilis	subtili	NOUN
ajst-25583	14	3	is	be	AUX
ajst-25583	14	4	a	a	DET
ajst-25583	14	5	gram	gram	NOUN
ajst-25583	14	6	-	-	PUNCT
ajst-25583	14	7	positive	positive	ADJ
ajst-25583	14	8	bacterium	bacterium	NOUN
ajst-25583	14	9	,	,	PUNCT
ajst-25583	14	10	which	which	PRON
ajst-25583	14	11	is	be	AUX
ajst-25583	14	12	capable	capable	ADJ
ajst-25583	14	13	of	of	ADP
ajst-25583	14	14	forming	form	VERB
ajst-25583	14	15	a	a	DET
ajst-25583	14	16	bacteriophage	bacteriophage	NOUN
ajst-25583	14	17	and	and	CCONJ
ajst-25583	14	18	has	have	VERB
ajst-25583	14	19	only	only	ADV
ajst-25583	14	20	one	one	NUM
ajst-25583	14	21	bacteriophage	bacteriophage	NOUN
ajst-25583	14	22	in	in	ADP
ajst-25583	14	23	the	the	DET
ajst-25583	14	24	ascospores	ascospore	NOUN
ajst-25583	14	25	.	.	PUNCT
ajst-25583	15	1	resistance	resistance	NOUN
ajst-25583	15	2	bacillus	bacillus	NOUN
ajst-25583	15	3	subtilis	subtili	NOUN
ajst-25583	15	4	has	have	AUX
ajst-25583	15	5	been	be	AUX
ajst-25583	15	6	widely	widely	ADV
ajst-25583	15	7	used	use	VERB
ajst-25583	15	8	and	and	CCONJ
ajst-25583	15	9	recognized	recognize	VERB
ajst-25583	15	10	as	as	ADP
ajst-25583	15	11	a	a	DET
ajst-25583	15	12	safe	safe	ADJ
ajst-25583	15	13	host	host	NOUN
ajst-25583	15	14	for	for	ADP
ajst-25583	15	15	the	the	DET
ajst-25583	15	16	production	production	NOUN
ajst-25583	15	17	of	of	ADP
ajst-25583	15	18	recombinant	recombinant	ADJ
ajst-25583	15	19	proteins	protein	NOUN
ajst-25583	15	20	,	,	PUNCT
ajst-25583	15	21	high	high	ADJ
ajst-25583	15	22	-	-	PUNCT
ajst-25583	15	23	value	value	NOUN
ajst-25583	15	24	chemicals	chemical	NOUN
ajst-25583	15	25	,	,	PUNCT
ajst-25583	15	26	and	and	CCONJ
ajst-25583	15	27	pharmaceuticals	pharmaceutical	NOUN
ajst-25583	15	28	,	,	PUNCT
ajst-25583	15	29	which	which	PRON
ajst-25583	15	30	contributes	contribute	VERB
ajst-25583	15	31	to	to	ADP
ajst-25583	15	32	the	the	DET
ajst-25583	15	33	easier	easy	ADJ
ajst-25583	15	34	purification	purification	NOUN
ajst-25583	15	35	of	of	ADP
ajst-25583	15	36	heterologous	heterologous	ADJ
ajst-25583	15	37	proteins	protein	NOUN
ajst-25583	15	38	or	or	CCONJ
ajst-25583	15	39	metabolites	metabolite	NOUN
ajst-25583	15	40	[	[	X
ajst-25583	15	41	5	5	NUM
ajst-25583	15	42	,	,	PUNCT
ajst-25583	15	43	6	6	NUM
ajst-25583	15	44	]	]	PUNCT
ajst-25583	15	45	.	.	PUNCT
ajst-25583	16	1	1.2	1.2	NUM
ajst-25583	16	2	.	.	PUNCT
ajst-25583	16	3	application	application	NOUN
ajst-25583	16	4	products	product	NOUN
ajst-25583	16	5	of	of	ADP
ajst-25583	16	6	bacillus	bacillus	NOUN
ajst-25583	16	7	subtilis	subtili	NOUN
ajst-25583	16	8	as	as	ADP
ajst-25583	16	9	an	an	DET
ajst-25583	16	10	engineered	engineer	VERB
ajst-25583	16	11	bacterium	bacterium	NOUN
ajst-25583	16	12	during	during	ADP
ajst-25583	16	13	the	the	DET
ajst-25583	16	14	development	development	NOUN
ajst-25583	16	15	of	of	ADP
ajst-25583	16	16	natural	natural	ADJ
ajst-25583	16	17	compound	compound	NOUN
ajst-25583	16	18	machine	machine	NOUN
ajst-25583	16	19	derivatives	derivative	NOUN
ajst-25583	16	20	,	,	PUNCT
ajst-25583	16	21	they	they	PRON
ajst-25583	16	22	mainly	mainly	ADV
ajst-25583	16	23	rely	rely	VERB
ajst-25583	16	24	on	on	ADP
ajst-25583	16	25	plant	plant	NOUN
ajst-25583	16	26	or	or	CCONJ
ajst-25583	16	27	animal	animal	NOUN
ajst-25583	16	28	extraction	extraction	NOUN
ajst-25583	16	29	,	,	PUNCT
ajst-25583	16	30	or	or	CCONJ
ajst-25583	16	31	chemical	chemical	NOUN
ajst-25583	16	32	synthesis	synthesis	NOUN
ajst-25583	16	33	,	,	PUNCT
ajst-25583	16	34	etc	etc	X
ajst-25583	16	35	.	.	X
ajst-25583	16	36	,	,	PUNCT
ajst-25583	16	37	which	which	PRON
ajst-25583	16	38	face	face	VERB
ajst-25583	16	39	problems	problem	NOUN
ajst-25583	16	40	such	such	ADJ
ajst-25583	16	41	as	as	ADP
ajst-25583	16	42	high	high	ADJ
ajst-25583	16	43	cost	cost	NOUN
ajst-25583	16	44	,	,	PUNCT
ajst-25583	16	45	low	low	ADJ
ajst-25583	16	46	quantity	quantity	NOUN
ajst-25583	16	47	,	,	PUNCT
ajst-25583	16	48	high	high	ADJ
ajst-25583	16	49	difficulty	difficulty	NOUN
ajst-25583	16	50	of	of	ADP
ajst-25583	16	51	purification	purification	NOUN
ajst-25583	16	52	,	,	PUNCT
ajst-25583	16	53	and	and	CCONJ
ajst-25583	16	54	ecological	ecological	ADJ
ajst-25583	16	55	damage	damage	NOUN
ajst-25583	16	56	,	,	PUNCT
ajst-25583	16	57	etc	etc	X
ajst-25583	16	58	.	.	X
ajst-25583	16	59	therefore	therefore	ADV
ajst-25583	16	60	,	,	PUNCT
ajst-25583	16	61	by	by	ADP
ajst-25583	16	62	using	use	VERB
ajst-25583	16	63	microorganisms	microorganism	NOUN
ajst-25583	16	64	as	as	ADP
ajst-25583	16	65	engineered	engineer	VERB
ajst-25583	16	66	cells	cell	NOUN
ajst-25583	16	67	for	for	ADP
ajst-25583	16	68	gene	gene	NOUN
ajst-25583	16	69	editing	editing	NOUN
ajst-25583	16	70	,	,	PUNCT
ajst-25583	16	71	so	so	SCONJ
ajst-25583	16	72	as	as	SCONJ
ajst-25583	16	73	to	to	PART
ajst-25583	16	74	re	re	VERB
ajst-25583	16	75	-	-	VERB
ajst-25583	16	76	construct	construct	VERB
ajst-25583	16	77	or	or	CCONJ
ajst-25583	16	78	optimize	optimize	VERB
ajst-25583	16	79	the	the	DET
ajst-25583	16	80	synthetic	synthetic	ADJ
ajst-25583	16	81	pathway	pathway	NOUN
ajst-25583	16	82	of	of	ADP
ajst-25583	16	83	the	the	DET
ajst-25583	16	84	target	target	NOUN
ajst-25583	16	85	products	product	NOUN
ajst-25583	16	86	,	,	PUNCT
ajst-25583	16	87	the	the	DET
ajst-25583	16	88	large	large	ADJ
ajst-25583	16	89	-	-	PUNCT
ajst-25583	16	90	scale	scale	NOUN
ajst-25583	16	91	preparation	preparation	NOUN
ajst-25583	16	92	of	of	ADP
ajst-25583	16	93	the	the	DET
ajst-25583	16	94	products	product	NOUN
ajst-25583	16	95	can	can	AUX
ajst-25583	16	96	be	be	AUX
ajst-25583	16	97	achieved	achieve	VERB
ajst-25583	16	98	.	.	PUNCT
ajst-25583	17	1	bacillus	bacillus	NOUN
ajst-25583	17	2	subtilis	subtili	NOUN
ajst-25583	17	3	,	,	PUNCT
ajst-25583	17	4	as	as	ADP
ajst-25583	17	5	an	an	DET
ajst-25583	17	6	excellent	excellent	ADJ
ajst-25583	17	7	host	host	NOUN
ajst-25583	17	8	for	for	ADP
ajst-25583	17	9	engineering	engineering	NOUN
ajst-25583	17	10	bacteria	bacteria	NOUN
ajst-25583	17	11	,	,	PUNCT
ajst-25583	17	12	is	be	AUX
ajst-25583	17	13	currently	currently	ADV
ajst-25583	17	14	favored	favor	VERB
ajst-25583	17	15	and	and	CCONJ
ajst-25583	17	16	has	have	AUX
ajst-25583	17	17	successfully	successfully	ADV
ajst-25583	17	18	synthesized	synthesize	VERB
ajst-25583	17	19	numerous	numerous	ADJ
ajst-25583	17	20	high	high	ADJ
ajst-25583	17	21	-	-	PUNCT
ajst-25583	17	22	value	value	NOUN
ajst-25583	17	23	products	product	NOUN
ajst-25583	17	24	,	,	PUNCT
ajst-25583	17	25	such	such	ADJ
ajst-25583	17	26	as	as	ADP
ajst-25583	17	27	squalene	squalene	NOUN
ajst-25583	17	28	[	[	X
ajst-25583	17	29	7	7	NUM
ajst-25583	17	30	]	]	PUNCT
ajst-25583	17	31	,	,	PUNCT
ajst-25583	17	32	echinocandin	echinocandin	VERB
ajst-25583	17	33	[	[	PRON
ajst-25583	17	34	8	8	NUM
ajst-25583	17	35	]	]	PUNCT
ajst-25583	17	36	,	,	PUNCT
ajst-25583	17	37	n	n	CCONJ
ajst-25583	17	38	-	-	PUNCT
ajst-25583	17	39	acetylglucosamine	acetylglucosamine	NOUN
ajst-25583	18	1	[	[	X
ajst-25583	18	2	9	9	NUM
ajst-25583	18	3	-	-	SYM
ajst-25583	18	4	12	12	NUM
ajst-25583	18	5	]	]	PUNCT
ajst-25583	18	6	,	,	PUNCT
ajst-25583	18	7	hyaluronic	hyaluronic	ADJ
ajst-25583	18	8	acid	acid	NOUN
ajst-25583	18	9	[	[	X
ajst-25583	18	10	13	13	NUM
ajst-25583	18	11	]	]	PUNCT
ajst-25583	18	12	,	,	PUNCT
ajst-25583	18	13	chondroitin	chondroitin	NOUN
ajst-25583	19	1	[	[	X
ajst-25583	19	2	14	14	NUM
ajst-25583	19	3	]	]	X
ajst-25583	19	4	,	,	PUNCT
ajst-25583	19	5	menaquinone-7	menaquinone-7	NUM
ajst-25583	19	6	[	[	X
ajst-25583	19	7	15	15	NUM
ajst-25583	19	8	,	,	PUNCT
ajst-25583	19	9	16	16	NUM
ajst-25583	19	10	]	]	PUNCT
ajst-25583	19	11	,	,	PUNCT
ajst-25583	19	12	riboflavin	riboflavin	NOUN
ajst-25583	19	13	[	[	PUNCT
ajst-25583	19	14	16	16	NUM
ajst-25583	19	15	,	,	PUNCT
ajst-25583	19	16	17	17	NUM
ajst-25583	19	17	]	]	PUNCT
ajst-25583	19	18	,	,	PUNCT
ajst-25583	19	19	and	and	CCONJ
ajst-25583	19	20	paclitaxel	paclitaxel	VERB
ajst-25583	20	1	[	[	X
ajst-25583	20	2	18	18	NUM
ajst-25583	20	3	]	]	PUNCT
ajst-25583	20	4	,	,	PUNCT
ajst-25583	20	5	among	among	ADP
ajst-25583	20	6	others	other	NOUN
ajst-25583	20	7	.	.	PUNCT
ajst-25583	21	1	2	2	X
ajst-25583	21	2	.	.	X
ajst-25583	21	3	crispr	crispr	ADJ
ajst-25583	21	4	2.1	2.1	NUM
ajst-25583	21	5	.	.	PUNCT
ajst-25583	21	6	introduction	introduction	NOUN
ajst-25583	21	7	to	to	ADP
ajst-25583	21	8	crispr	crispr	PROPN
ajst-25583	21	9	crispr	crispr	NOUN
ajst-25583	21	10	,	,	PUNCT
ajst-25583	21	11	an	an	DET
ajst-25583	21	12	acquired	acquire	VERB
ajst-25583	21	13	immune	immune	ADJ
ajst-25583	21	14	system	system	NOUN
ajst-25583	21	15	for	for	ADP
ajst-25583	21	16	bacteria	bacteria	NOUN
ajst-25583	21	17	and	and	CCONJ
ajst-25583	21	18	archaea	archaea	NOUN
ajst-25583	21	19	to	to	PART
ajst-25583	21	20	defend	defend	VERB
ajst-25583	21	21	themselves	themselves	PRON
ajst-25583	21	22	against	against	ADP
ajst-25583	21	23	invasion	invasion	NOUN
ajst-25583	21	24	by	by	ADP
ajst-25583	21	25	foreign	foreign	ADJ
ajst-25583	21	26	viruses	virus	NOUN
ajst-25583	21	27	,	,	PUNCT
ajst-25583	21	28	has	have	AUX
ajst-25583	21	29	now	now	ADV
ajst-25583	21	30	evolved	evolve	VERB
ajst-25583	21	31	into	into	ADP
ajst-25583	21	32	a	a	DET
ajst-25583	21	33	powerful	powerful	ADJ
ajst-25583	21	34	gene	gene	NOUN
ajst-25583	21	35	editing	edit	VERB
ajst-25583	21	36	tool	tool	NOUN
ajst-25583	21	37	in	in	ADP
ajst-25583	21	38	genetic	genetic	ADJ
ajst-25583	21	39	engineering	engineering	NOUN
ajst-25583	21	40	.	.	PUNCT
ajst-25583	22	1	when	when	SCONJ
ajst-25583	22	2	a	a	DET
ajst-25583	22	3	bacterium	bacterium	NOUN
ajst-25583	22	4	is	be	AUX
ajst-25583	22	5	infected	infect	VERB
ajst-25583	22	6	by	by	ADP
ajst-25583	22	7	an	an	DET
ajst-25583	22	8	exogenous	exogenous	ADJ
ajst-25583	22	9	phage	phage	NOUN
ajst-25583	22	10	,	,	PUNCT
ajst-25583	22	11	a	a	DET
ajst-25583	22	12	fragment	fragment	NOUN
ajst-25583	22	13	of	of	ADP
ajst-25583	22	14	the	the	DET
ajst-25583	22	15	phage	phage	NOUN
ajst-25583	22	16	genome	genome	NOUN
ajst-25583	22	17	dna	dna	NOUN
ajst-25583	22	18	is	be	AUX
ajst-25583	22	19	recognized	recognize	VERB
ajst-25583	22	20	and	and	CCONJ
ajst-25583	22	21	integrated	integrate	VERB
ajst-25583	22	22	into	into	ADP
ajst-25583	22	23	crispr	crispr	NOUN
ajst-25583	22	24	,	,	PUNCT
ajst-25583	22	25	and	and	CCONJ
ajst-25583	22	26	the	the	DET
ajst-25583	22	27	corresponding	correspond	VERB
ajst-25583	22	28	crrna	crrna	NOUN
ajst-25583	22	29	precursor	precursor	NOUN
ajst-25583	22	30	(	(	PUNCT
ajst-25583	22	31	pre	pre	ADJ
ajst-25583	22	32	-	-	NOUN
ajst-25583	22	33	crrna	crrna	NOUN
ajst-25583	22	34	)	)	PUNCT
ajst-25583	22	35	is	be	AUX
ajst-25583	22	36	transcribed	transcribe	VERB
ajst-25583	22	37	.	.	PUNCT
ajst-25583	23	1	pre	pre	ADJ
ajst-25583	23	2	-	-	ADJ
ajst-25583	23	3	crrna	crrna	NOUN
ajst-25583	23	4	is	be	AUX
ajst-25583	23	5	modified	modify	VERB
ajst-25583	23	6	to	to	PART
ajst-25583	23	7	generate	generate	VERB
ajst-25583	23	8	guide	guide	NOUN
ajst-25583	23	9	rna	rna	NOUN
ajst-25583	23	10	(	(	PUNCT
ajst-25583	23	11	grna	grna	NOUN
ajst-25583	23	12	,	,	PUNCT
ajst-25583	23	13	guiderna	guiderna	NOUN
ajst-25583	23	14	)	)	PUNCT
ajst-25583	23	15	.	.	PUNCT
ajst-25583	24	1	grna	grna	NOUN
ajst-25583	24	2	contains	contain	VERB
ajst-25583	24	3	dna	dna	NOUN
ajst-25583	24	4	from	from	ADP
ajst-25583	24	5	the	the	DET
ajst-25583	24	6	phage	phage	NOUN
ajst-25583	24	7	genome	genome	NOUN
ajst-25583	24	8	,	,	PUNCT
ajst-25583	24	9	and	and	CCONJ
ajst-25583	24	10	can	can	AUX
ajst-25583	24	11	recognize	recognize	VERB
ajst-25583	24	12	the	the	DET
ajst-25583	24	13	phage	phage	NOUN
ajst-25583	24	14	genome	genome	NOUN
ajst-25583	24	15	through	through	ADP
ajst-25583	24	16	the	the	DET
ajst-25583	24	17	principle	principle	NOUN
ajst-25583	24	18	of	of	ADP
ajst-25583	24	19	basecomplementary	basecomplementary	ADJ
ajst-25583	24	20	pairing	pairing	NOUN
ajst-25583	24	21	.	.	PUNCT
ajst-25583	25	1	the	the	DET
ajst-25583	25	2	grna	grna	NOUN
ajst-25583	25	3	contains	contain	VERB
ajst-25583	25	4	dna	dna	NOUN
ajst-25583	25	5	from	from	ADP
ajst-25583	25	6	the	the	DET
ajst-25583	25	7	phage	phage	NOUN
ajst-25583	25	8	genome	genome	NOUN
ajst-25583	25	9	and	and	CCONJ
ajst-25583	25	10	can	can	AUX
ajst-25583	25	11	recognize	recognize	VERB
ajst-25583	25	12	the	the	DET
ajst-25583	25	13	phage	phage	NOUN
ajst-25583	25	14	genome	genome	NOUN
ajst-25583	25	15	through	through	ADP
ajst-25583	25	16	the	the	DET
ajst-25583	25	17	base	base	ADJ
ajst-25583	25	18	complementary	complementary	ADJ
ajst-25583	25	19	pairing	pairing	NOUN
ajst-25583	25	20	principle	principle	NOUN
ajst-25583	25	21	.	.	PUNCT
ajst-25583	26	1	at	at	ADP
ajst-25583	26	2	this	this	DET
ajst-25583	26	3	time	time	NOUN
ajst-25583	26	4	,	,	PUNCT
ajst-25583	26	5	grna	grna	NOUN
ajst-25583	26	6	and	and	CCONJ
ajst-25583	26	7	cas	cas	NOUN
ajst-25583	26	8	protein	protein	NOUN
ajst-25583	26	9	specifically	specifically	ADV
ajst-25583	26	10	bind	bind	VERB
ajst-25583	26	11	to	to	PART
ajst-25583	26	12	recognize	recognize	VERB
ajst-25583	26	13	the	the	DET
ajst-25583	26	14	phage	phage	NOUN
ajst-25583	26	15	genome	genome	NOUN
ajst-25583	26	16	to	to	PART
ajst-25583	26	17	cut	cut	VERB
ajst-25583	26	18	dna	dna	PROPN
ajst-25583	26	19	.	.	PUNCT
ajst-25583	27	1	crispr	crispr	PROPN
ajst-25583	27	2	gene	gene	NOUN
ajst-25583	27	3	editing	edit	VERB
ajst-25583	27	4	system	system	NOUN
ajst-25583	27	5	is	be	AUX
ajst-25583	27	6	to	to	PART
ajst-25583	27	7	guide	guide	VERB
ajst-25583	27	8	cas	cas	NOUN
ajst-25583	27	9	protein	protein	NOUN
ajst-25583	27	10	(	(	PUNCT
ajst-25583	27	11	e.g.	e.g.	ADV
ajst-25583	27	12	cas9	cas9	NOUN
ajst-25583	27	13	,	,	PUNCT
ajst-25583	27	14	cpf1	cpf1	PROPN
ajst-25583	27	15	,	,	PUNCT
ajst-25583	27	16	etc	etc	X
ajst-25583	27	17	.	.	X
ajst-25583	27	18	)	)	PUNCT
ajst-25583	28	1	through	through	ADP
ajst-25583	28	2	sgrna	sgrna	NOUN
ajst-25583	28	3	to	to	PART
ajst-25583	28	4	locate	locate	VERB
ajst-25583	28	5	to	to	ADP
ajst-25583	28	6	the	the	DET
ajst-25583	28	7	target	target	NOUN
ajst-25583	28	8	gene	gene	NOUN
ajst-25583	28	9	site	site	NOUN
ajst-25583	28	10	,	,	PUNCT
ajst-25583	28	11	and	and	CCONJ
ajst-25583	28	12	then	then	ADV
ajst-25583	28	13	cas	cas	PROPN
ajst-25583	28	14	protein	protein	NOUN
ajst-25583	28	15	cuts	cut	VERB
ajst-25583	28	16	the	the	DET
ajst-25583	28	17	dna	dna	NOUN
ajst-25583	28	18	at	at	ADP
ajst-25583	28	19	the	the	DET
ajst-25583	28	20	specific	specific	ADJ
ajst-25583	28	21	site	site	NOUN
ajst-25583	28	22	in	in	ADP
ajst-25583	28	23	the	the	DET
ajst-25583	28	24	genome	genome	NOUN
ajst-25583	28	25	,	,	PUNCT
ajst-25583	28	26	resulting	result	VERB
ajst-25583	28	27	in	in	ADP
ajst-25583	28	28	dna	dna	PROPN
ajst-25583	28	29	doublestranded	doublestrande	VERB
ajst-25583	28	30	breaks	break	NOUN
ajst-25583	28	31	,	,	PUNCT
ajst-25583	28	32	and	and	CCONJ
ajst-25583	28	33	in	in	ADP
ajst-25583	28	34	the	the	DET
ajst-25583	28	35	process	process	NOUN
ajst-25583	28	36	of	of	ADP
ajst-25583	28	37	homology	homology	NOUN
ajst-25583	28	38	-	-	PUNCT
ajst-25583	28	39	directed	direct	VERB
ajst-25583	28	40	repair	repair	NOUN
ajst-25583	28	41	introduction	introduction	NOUN
ajst-25583	28	42	of	of	ADP
ajst-25583	28	43	specific	specific	ADJ
ajst-25583	28	44	modified	modify	VERB
ajst-25583	28	45	fragments	fragment	NOUN
ajst-25583	28	46	into	into	ADP
ajst-25583	28	47	the	the	DET
ajst-25583	28	48	genome	genome	NOUN
ajst-25583	28	49	through	through	ADP
ajst-25583	28	50	homology	homology	NOUN
ajst-25583	28	51	arms	arm	NOUN
ajst-25583	28	52	,	,	PUNCT
ajst-25583	28	53	resulting	result	VERB
ajst-25583	28	54	in	in	ADP
ajst-25583	28	55	gene	gene	NOUN
ajst-25583	28	56	knockout	knockout	NOUN
ajst-25583	28	57	,	,	PUNCT
ajst-25583	28	58	insertion	insertion	NOUN
ajst-25583	28	59	or	or	CCONJ
ajst-25583	28	60	mutation	mutation	NOUN
ajst-25583	28	61	[	[	X
ajst-25583	28	62	19	19	NUM
ajst-25583	28	63	]	]	PUNCT
ajst-25583	28	64	.	.	PUNCT
ajst-25583	29	1	2.2	2.2	NUM
ajst-25583	29	2	.	.	PUNCT
ajst-25583	29	3	history	history	NOUN
ajst-25583	29	4	of	of	ADP
ajst-25583	29	5	crispr	crispr	ADJ
ajst-25583	29	6	technology	technology	NOUN
ajst-25583	29	7	in	in	ADP
ajst-25583	29	8	bacillus	bacillus	NOUN
ajst-25583	29	9	subtilis	subtili	NOUN
ajst-25583	29	10	bacillus	bacillus	NOUN
ajst-25583	29	11	subtilis	subtili	NOUN
ajst-25583	29	12	is	be	AUX
ajst-25583	29	13	a	a	DET
ajst-25583	29	14	typical	typical	ADJ
ajst-25583	29	15	gram	gram	ADJ
ajst-25583	29	16	-	-	PUNCT
ajst-25583	29	17	positive	positive	ADJ
ajst-25583	29	18	bacterium	bacterium	NOUN
ajst-25583	29	19	and	and	CCONJ
ajst-25583	29	20	has	have	AUX
ajst-25583	29	21	been	be	AUX
ajst-25583	29	22	recognized	recognize	VERB
ajst-25583	29	23	as	as	ADP
ajst-25583	29	24	a	a	DET
ajst-25583	29	25	safe	safe	ADJ
ajst-25583	29	26	strain	strain	NOUN
ajst-25583	29	27	(	(	PUNCT
ajst-25583	29	28	gras	gras	PROPN
ajst-25583	29	29	)	)	PUNCT
ajst-25583	29	30	with	with	ADP
ajst-25583	29	31	superior	superior	ADJ
ajst-25583	29	32	protein	protein	NOUN
ajst-25583	29	33	secretion	secretion	NOUN
ajst-25583	29	34	capacity	capacity	NOUN
ajst-25583	29	35	compared	compare	VERB
ajst-25583	29	36	to	to	ADP
ajst-25583	29	37	other	other	ADJ
ajst-25583	29	38	microorganisms	microorganism	NOUN
ajst-25583	29	39	.	.	PUNCT
ajst-25583	30	1	b.	b.	PROPN
ajst-25583	30	2	subtilis	subtilis	PROPN
ajst-25583	30	3	has	have	VERB
ajst-25583	30	4	a	a	DET
ajst-25583	30	5	clear	clear	ADJ
ajst-25583	30	6	genetic	genetic	ADJ
ajst-25583	30	7	background	background	NOUN
ajst-25583	30	8	and	and	CCONJ
ajst-25583	30	9	a	a	DET
ajst-25583	30	10	wellestablished	wellestablished	ADJ
ajst-25583	30	11	platform	platform	NOUN
ajst-25583	30	12	for	for	ADP
ajst-25583	30	13	molecular	molecular	ADJ
ajst-25583	30	14	modification	modification	NOUN
ajst-25583	30	15	[	[	X
ajst-25583	30	16	20	20	NUM
ajst-25583	30	17	-	-	SYM
ajst-25583	30	18	23	23	NUM
ajst-25583	30	19	]	]	PUNCT
ajst-25583	30	20	.	.	PUNCT
ajst-25583	31	1	the	the	DET
ajst-25583	31	2	creation	creation	NOUN
ajst-25583	31	3	and	and	CCONJ
ajst-25583	31	4	expansion	expansion	NOUN
ajst-25583	31	5	of	of	ADP
ajst-25583	31	6	databases	database	NOUN
ajst-25583	31	7	such	such	ADJ
ajst-25583	31	8	as	as	ADP
ajst-25583	31	9	bsubcyc	bsubcyc	NOUN
ajst-25583	31	10	,	,	PUNCT
ajst-25583	31	11	subtiwiki	subtiwiki	PROPN
ajst-25583	31	12	,	,	PUNCT
ajst-25583	31	13	dbtbs	dbtbs	PROPN
ajst-25583	31	14	,	,	PUNCT
ajst-25583	31	15	etc	etc	X
ajst-25583	31	16	.	.	X
ajst-25583	31	17	have	have	AUX
ajst-25583	31	18	allowed	allow	VERB
ajst-25583	31	19	us	we	PRON
ajst-25583	31	20	to	to	PART
ajst-25583	31	21	conveniently	conveniently	ADV
ajst-25583	31	22	query	query	VERB
ajst-25583	31	23	genetic	genetic	ADJ
ajst-25583	31	24	and	and	CCONJ
ajst-25583	31	25	metabolism	metabolism	NOUN
ajst-25583	31	26	-	-	PUNCT
ajst-25583	31	27	related	relate	VERB
ajst-25583	31	28	information	information	NOUN
ajst-25583	31	29	of	of	ADP
ajst-25583	31	30	b.	b.	PROPN
ajst-25583	31	31	subtilis	subtilis	PROPN
ajst-25583	32	1	[	[	X
ajst-25583	32	2	24	24	NUM
ajst-25583	32	3	,	,	PUNCT
ajst-25583	32	4	25	25	NUM
ajst-25583	32	5	]	]	PUNCT
ajst-25583	32	6	.	.	PUNCT
ajst-25583	33	1	in	in	ADP
ajst-25583	33	2	summary	summary	NOUN
ajst-25583	33	3	,	,	PUNCT
ajst-25583	33	4	bacillus	bacillus	NOUN
ajst-25583	33	5	subtilis	subtili	NOUN
ajst-25583	33	6	becomes	become	VERB
ajst-25583	33	7	a	a	DET
ajst-25583	33	8	powerful	powerful	ADJ
ajst-25583	33	9	host	host	NOUN
ajst-25583	33	10	capable	capable	ADJ
ajst-25583	33	11	of	of	ADP
ajst-25583	33	12	producing	produce	VERB
ajst-25583	33	13	platform	platform	NOUN
ajst-25583	33	14	chemicals	chemical	NOUN
ajst-25583	33	15	,	,	PUNCT
ajst-25583	33	16	biopolymers	biopolymer	NOUN
ajst-25583	33	17	,	,	PUNCT
ajst-25583	33	18	and	and	CCONJ
ajst-25583	33	19	proteins	protein	NOUN
ajst-25583	33	20	.	.	PUNCT
ajst-25583	34	1	currently	currently	ADV
ajst-25583	34	2	,	,	PUNCT
ajst-25583	34	3	bacillus	bacillus	NOUN
ajst-25583	34	4	subtilis	subtili	NOUN
ajst-25583	34	5	has	have	AUX
ajst-25583	34	6	been	be	AUX
ajst-25583	34	7	used	use	VERB
ajst-25583	34	8	to	to	PART
ajst-25583	34	9	produce	produce	VERB
ajst-25583	34	10	a	a	DET
ajst-25583	34	11	variety	variety	NOUN
ajst-25583	34	12	of	of	ADP
ajst-25583	34	13	products	product	NOUN
ajst-25583	34	14	such	such	ADJ
ajst-25583	34	15	as	as	ADP
ajst-25583	34	16	riboflavin	riboflavin	NOUN
ajst-25583	34	17	,	,	PUNCT
ajst-25583	34	18	chondroitin	chondroitin	NOUN
ajst-25583	34	19	,	,	PUNCT
ajst-25583	34	20	hyaluronic	hyaluronic	ADJ
ajst-25583	34	21	acid	acid	NOUN
ajst-25583	34	22	,	,	PUNCT
ajst-25583	34	23	and	and	CCONJ
ajst-25583	34	24	poly	poly	ADJ
ajst-25583	34	25	-	-	PUNCT
ajst-25583	34	26	y	y	NOUN
ajst-25583	34	27	-	-	PUNCT
ajst-25583	34	28	glutamic	glutamic	ADJ
ajst-25583	34	29	acid	acid	NOUN
ajst-25583	35	1	[	[	X
ajst-25583	35	2	26	26	NUM
ajst-25583	35	3	,	,	PUNCT
ajst-25583	35	4	103	103	NUM
ajst-25583	35	5	27	27	NUM
ajst-25583	35	6	]	]	PUNCT
ajst-25583	35	7	.	.	PUNCT
ajst-25583	36	1	as	as	ADP
ajst-25583	36	2	a	a	DET
ajst-25583	36	3	cell	cell	NOUN
ajst-25583	36	4	factory	factory	NOUN
ajst-25583	36	5	,	,	PUNCT
ajst-25583	36	6	bacillus	bacillus	NOUN
ajst-25583	36	7	subtilis	subtili	NOUN
ajst-25583	36	8	needs	need	VERB
ajst-25583	36	9	convenient	convenient	ADJ
ajst-25583	36	10	and	and	CCONJ
ajst-25583	36	11	efficient	efficient	ADJ
ajst-25583	36	12	gene	gene	NOUN
ajst-25583	36	13	editing	edit	VERB
ajst-25583	36	14	tools	tool	NOUN
ajst-25583	36	15	to	to	PART
ajst-25583	36	16	modify	modify	VERB
ajst-25583	36	17	its	its	PRON
ajst-25583	36	18	genome	genome	NOUN
ajst-25583	36	19	-	-	PUNCT
ajst-25583	36	20	related	relate	VERB
ajst-25583	36	21	genes	gene	NOUN
ajst-25583	36	22	as	as	ADV
ajst-25583	36	23	well	well	ADV
ajst-25583	36	24	as	as	ADP
ajst-25583	36	25	to	to	PART
ajst-25583	36	26	solve	solve	VERB
ajst-25583	36	27	problems	problem	NOUN
ajst-25583	36	28	such	such	ADJ
ajst-25583	36	29	as	as	ADP
ajst-25583	36	30	genetic	genetic	ADJ
ajst-25583	36	31	instability	instability	NOUN
ajst-25583	36	32	of	of	ADP
ajst-25583	36	33	free	free	ADJ
ajst-25583	36	34	plasmids	plasmid	NOUN
ajst-25583	36	35	.	.	PUNCT
ajst-25583	37	1	before	before	ADP
ajst-25583	37	2	the	the	DET
ajst-25583	37	3	emergence	emergence	NOUN
ajst-25583	37	4	of	of	ADP
ajst-25583	37	5	crispr	crispr	ADJ
ajst-25583	37	6	technology	technology	NOUN
ajst-25583	37	7	,	,	PUNCT
ajst-25583	37	8	the	the	DET
ajst-25583	37	9	commonly	commonly	ADV
ajst-25583	37	10	used	use	VERB
ajst-25583	37	11	gene	gene	NOUN
ajst-25583	37	12	editing	editing	NOUN
ajst-25583	37	13	tools	tool	NOUN
ajst-25583	37	14	are	be	AUX
ajst-25583	37	15	antiselective	antiselective	ADJ
ajst-25583	37	16	marker	marker	NOUN
ajst-25583	37	17	system	system	NOUN
ajst-25583	37	18	and	and	CCONJ
ajst-25583	37	19	site	site	NOUN
ajst-25583	37	20	-	-	PUNCT
ajst-25583	37	21	specific	specific	ADJ
ajst-25583	37	22	recombination	recombination	NOUN
ajst-25583	37	23	system	system	NOUN
ajst-25583	37	24	.	.	PUNCT
ajst-25583	38	1	they	they	PRON
ajst-25583	38	2	are	be	AUX
ajst-25583	38	3	inefficient	inefficient	ADJ
ajst-25583	38	4	and	and	CCONJ
ajst-25583	38	5	complicated	complicated	ADJ
ajst-25583	38	6	in	in	ADP
ajst-25583	38	7	the	the	DET
ajst-25583	38	8	process	process	NOUN
ajst-25583	38	9	of	of	ADP
ajst-25583	38	10	editing	edit	VERB
ajst-25583	38	11	the	the	DET
ajst-25583	38	12	genome	genome	NOUN
ajst-25583	38	13	[	[	X
ajst-25583	38	14	28	28	NUM
ajst-25583	38	15	-	-	SYM
ajst-25583	38	16	30	30	NUM
ajst-25583	38	17	]	]	PUNCT
ajst-25583	38	18	.	.	PUNCT
ajst-25583	39	1	with	with	ADP
ajst-25583	39	2	the	the	DET
ajst-25583	39	3	gradual	gradual	ADJ
ajst-25583	39	4	development	development	NOUN
ajst-25583	39	5	of	of	ADP
ajst-25583	39	6	crispr	crispr	ADJ
ajst-25583	39	7	-	-	PUNCT
ajst-25583	39	8	cas	cas	NOUN
ajst-25583	39	9	technology	technology	NOUN
ajst-25583	39	10	,	,	PUNCT
ajst-25583	39	11	several	several	ADJ
ajst-25583	39	12	efficient	efficient	ADJ
ajst-25583	39	13	crispr	crispr	NOUN
ajst-25583	39	14	systems	system	NOUN
ajst-25583	39	15	have	have	AUX
ajst-25583	39	16	been	be	AUX
ajst-25583	39	17	developed	develop	VERB
ajst-25583	39	18	in	in	ADP
ajst-25583	39	19	bacillus	bacillus	NOUN
ajst-25583	39	20	subtilis	subtili	NOUN
ajst-25583	39	21	,	,	PUNCT
ajst-25583	39	22	and	and	CCONJ
ajst-25583	39	23	their	their	PRON
ajst-25583	39	24	development	development	NOUN
ajst-25583	39	25	will	will	AUX
ajst-25583	39	26	be	be	AUX
ajst-25583	39	27	systematically	systematically	ADV
ajst-25583	39	28	described	describe	VERB
ajst-25583	39	29	below	below	ADV
ajst-25583	39	30	.	.	PUNCT
ajst-25583	40	1	altenbuchner	altenbuchner	PROPN
ajst-25583	40	2	constructed	construct	VERB
ajst-25583	40	3	a	a	DET
ajst-25583	40	4	single	single	ADJ
ajst-25583	40	5	-	-	PUNCT
ajst-25583	40	6	plasmid	plasmid	ADJ
ajst-25583	40	7	crispr	crispr	ADJ
ajst-25583	40	8	-	-	PUNCT
ajst-25583	40	9	cas9	cas9	NOUN
ajst-25583	40	10	system	system	NOUN
ajst-25583	40	11	,	,	PUNCT
ajst-25583	40	12	which	which	PRON
ajst-25583	40	13	is	be	AUX
ajst-25583	40	14	the	the	DET
ajst-25583	40	15	first	first	ADJ
ajst-25583	40	16	application	application	NOUN
ajst-25583	40	17	of	of	ADP
ajst-25583	40	18	crispr	crispr	ADJ
ajst-25583	40	19	system	system	NOUN
ajst-25583	40	20	in	in	ADP
ajst-25583	40	21	bacillus	bacillus	NOUN
ajst-25583	40	22	subtilis	subtili	NOUN
ajst-25583	40	23	.	.	PUNCT
ajst-25583	41	1	grnas	grna	NOUN
ajst-25583	41	2	can	can	AUX
ajst-25583	41	3	be	be	AUX
ajst-25583	41	4	rapidly	rapidly	ADV
ajst-25583	41	5	constructed	construct	VERB
ajst-25583	41	6	into	into	ADP
ajst-25583	41	7	editing	edit	VERB
ajst-25583	41	8	plasmids	plasmid	NOUN
ajst-25583	41	9	by	by	ADP
ajst-25583	41	10	restriction	restriction	NOUN
ajst-25583	41	11	endonuclease	endonuclease	NOUN
ajst-25583	41	12	bsal	bsal	NOUN
ajst-25583	41	13	combined	combine	VERB
ajst-25583	41	14	with	with	ADP
ajst-25583	41	15	blue	blue	ADJ
ajst-25583	41	16	-	-	PUNCT
ajst-25583	41	17	white	white	ADJ
ajst-25583	41	18	-	-	PUNCT
ajst-25583	41	19	spotting	spot	VERB
ajst-25583	41	20	screening	screening	NOUN
ajst-25583	41	21	,	,	PUNCT
ajst-25583	41	22	and	and	CCONJ
ajst-25583	41	23	the	the	DET
ajst-25583	41	24	function	function	NOUN
ajst-25583	41	25	of	of	ADP
ajst-25583	41	26	crispr	crispr	ADJ
ajst-25583	41	27	-	-	PUNCT
ajst-25583	41	28	cas9	cas9	NOUN
ajst-25583	41	29	vectors	vector	NOUN
ajst-25583	41	30	can	can	AUX
ajst-25583	41	31	be	be	AUX
ajst-25583	41	32	performed	perform	VERB
ajst-25583	41	33	by	by	ADP
ajst-25583	41	34	introduction	introduction	NOUN
ajst-25583	41	35	of	of	ADP
ajst-25583	41	36	two	two	NUM
ajst-25583	41	37	large	large	ADJ
ajst-25583	41	38	deletions	deletion	NOUN
ajst-25583	41	39	and	and	CCONJ
ajst-25583	41	40	repair	repair	NOUN
ajst-25583	41	41	of	of	ADP
ajst-25583	41	42	the	the	DET
ajst-25583	41	43	trpc2	trpc2	NOUN
ajst-25583	41	44	mutation	mutation	NOUN
ajst-25583	41	45	in	in	ADP
ajst-25583	41	46	bacillus	bacillus	NOUN
ajst-25583	41	47	subtilis	subtili	NOUN
ajst-25583	41	48	168.westbrook	168.westbrook	NUM
ajst-25583	41	49	et	et	NOUN
ajst-25583	41	50	al	al	PROPN
ajst-25583	41	51	.	.	PROPN
ajst-25583	41	52	constructed	construct	VERB
ajst-25583	41	53	a	a	DET
ajst-25583	41	54	crisprcas9	crisprcas9	NOUN
ajst-25583	41	55	-	-	PUNCT
ajst-25583	41	56	based	base	VERB
ajst-25583	41	57	genome	genome	NOUN
ajst-25583	41	58	editing	edit	VERB
ajst-25583	41	59	toolkit	toolkit	NOUN
ajst-25583	41	60	for	for	ADP
ajst-25583	41	61	bacillus	bacillus	NOUN
ajst-25583	41	62	subtilis	subtili	NOUN
ajst-25583	41	63	,	,	PUNCT
ajst-25583	41	64	which	which	PRON
ajst-25583	41	65	integrated	integrate	VERB
ajst-25583	41	66	the	the	DET
ajst-25583	41	67	cas9	cas9	NOUN
ajst-25583	41	68	protein	protein	NOUN
ajst-25583	41	69	and	and	CCONJ
ajst-25583	41	70	grna	grna	NOUN
ajst-25583	41	71	expression	expression	NOUN
ajst-25583	41	72	frames	frame	NOUN
ajst-25583	41	73	into	into	ADP
ajst-25583	41	74	the	the	DET
ajst-25583	41	75	genome	genome	NOUN
ajst-25583	41	76	in	in	ADP
ajst-25583	41	77	an	an	DET
ajst-25583	41	78	integrative	integrative	ADJ
ajst-25583	41	79	manner	manner	NOUN
ajst-25583	41	80	to	to	PART
ajst-25583	41	81	achieve	achieve	VERB
ajst-25583	41	82	single	single	ADJ
ajst-25583	41	83	-	-	PUNCT
ajst-25583	41	84	site	site	NOUN
ajst-25583	41	85	and	and	CCONJ
ajst-25583	41	86	two	two	NUM
ajst-25583	41	87	-	-	PUNCT
ajst-25583	41	88	site	site	NOUN
ajst-25583	41	89	mutations	mutation	NOUN
ajst-25583	41	90	on	on	ADP
ajst-25583	41	91	the	the	DET
ajst-25583	41	92	genome	genome	NOUN
ajst-25583	41	93	,	,	PUNCT
ajst-25583	41	94	with	with	ADP
ajst-25583	41	95	efficiencies	efficiency	NOUN
ajst-25583	41	96	of	of	ADP
ajst-25583	41	97	up	up	ADP
ajst-25583	41	98	to	to	PART
ajst-25583	41	99	100	100	NUM
ajst-25583	41	100	%	%	NOUN
ajst-25583	41	101	and	and	CCONJ
ajst-25583	41	102	85	85	NUM
ajst-25583	41	103	%	%	NOUN
ajst-25583	41	104	,	,	PUNCT
ajst-25583	41	105	respectively	respectively	ADV
ajst-25583	41	106	.	.	PUNCT
ajst-25583	42	1	and	and	CCONJ
ajst-25583	42	2	the	the	DET
ajst-25583	42	3	hyaluronan	hyaluronan	PROPN
ajst-25583	42	4	synthase	synthase	NOUN
ajst-25583	42	5	gene	gene	NOUN
ajst-25583	42	6	,	,	PUNCT
ajst-25583	42	7	which	which	PRON
ajst-25583	42	8	is	be	AUX
ajst-25583	42	9	2.9	2.9	NUM
ajst-25583	42	10	kb	kb	NOUN
ajst-25583	42	11	long	long	ADV
ajst-25583	42	12	,	,	PUNCT
ajst-25583	42	13	was	be	AUX
ajst-25583	42	14	integrated	integrate	VERB
ajst-25583	42	15	into	into	ADP
ajst-25583	42	16	the	the	DET
ajst-25583	42	17	genome	genome	NOUN
ajst-25583	42	18	with	with	ADP
ajst-25583	42	19	an	an	DET
ajst-25583	42	20	efficiency	efficiency	NOUN
ajst-25583	42	21	of	of	ADP
ajst-25583	42	22	69	69	NUM
ajst-25583	42	23	%	%	NOUN
ajst-25583	42	24	[	[	X
ajst-25583	42	25	31].zhang	31].zhang	PROPN
ajst-25583	42	26	et	et	PROPN
ajst-25583	42	27	al	al	PROPN
ajst-25583	42	28	.	.	PROPN
ajst-25583	42	29	constructed	construct	VERB
ajst-25583	42	30	a	a	DET
ajst-25583	42	31	dual	dual	ADJ
ajst-25583	42	32	-	-	PUNCT
ajst-25583	42	33	plasmid	plasmid	ADJ
ajst-25583	42	34	crispr	crispr	ADJ
ajst-25583	42	35	-	-	PUNCT
ajst-25583	42	36	cas9	cas9	NOUN
ajst-25583	42	37	system	system	NOUN
ajst-25583	42	38	and	and	CCONJ
ajst-25583	42	39	utilized	utilize	VERB
ajst-25583	42	40	its	its	PRON
ajst-25583	42	41	highly	highly	ADV
ajst-25583	42	42	efficient	efficient	ADJ
ajst-25583	42	43	genome	genome	NOUN
ajst-25583	42	44	editing	edit	VERB
ajst-25583	42	45	ability	ability	NOUN
ajst-25583	42	46	to	to	PART
ajst-25583	42	47	knock	knock	VERB
ajst-25583	42	48	out	out	ADP
ajst-25583	42	49	five	five	NUM
ajst-25583	42	50	genes	gene	NOUN
ajst-25583	42	51	on	on	ADP
ajst-25583	42	52	the	the	DET
ajst-25583	42	53	genome	genome	NOUN
ajst-25583	42	54	of	of	ADP
ajst-25583	42	55	bacillus	bacillus	NOUN
ajst-25583	42	56	subtilis	subtili	NOUN
ajst-25583	42	57	atcc6051a	atcc6051a	NOUN
ajst-25583	42	58	:	:	PUNCT
ajst-25583	42	59	srfc	srfc	PROPN
ajst-25583	42	60	,	,	PUNCT
ajst-25583	42	61	spoiiac	spoiiac	NOUN
ajst-25583	42	62	,	,	PUNCT
ajst-25583	42	63	npre	npre	PROPN
ajst-25583	42	64	,	,	PUNCT
ajst-25583	42	65	apre	apre	PROPN
ajst-25583	42	66	and	and	CCONJ
ajst-25583	42	67	amye	amye	NOUN
ajst-25583	42	68	,	,	PUNCT
ajst-25583	42	69	which	which	PRON
ajst-25583	42	70	solved	solve	VERB
ajst-25583	42	71	the	the	DET
ajst-25583	42	72	problems	problem	NOUN
ajst-25583	42	73	of	of	ADP
ajst-25583	42	74	foam	foam	NOUN
ajst-25583	42	75	production	production	NOUN
ajst-25583	42	76	and	and	CCONJ
ajst-25583	42	77	bacillus	bacillus	NOUN
ajst-25583	42	78	production	production	NOUN
ajst-25583	42	79	in	in	ADP
ajst-25583	42	80	the	the	DET
ajst-25583	42	81	industrial	industrial	ADJ
ajst-25583	42	82	enzyme	enzyme	NOUN
ajst-25583	42	83	in	in	ADP
ajst-25583	42	84	the	the	DET
ajst-25583	42	85	production	production	NOUN
ajst-25583	42	86	process	process	NOUN
ajst-25583	43	1	[	[	X
ajst-25583	43	2	32].so	32].so	NUM
ajst-25583	43	3	et	et	NOUN
ajst-25583	43	4	al	al	PROPN
ajst-25583	43	5	.	.	PROPN
ajst-25583	43	6	achieved	achieve	VERB
ajst-25583	43	7	the	the	DET
ajst-25583	43	8	knockdown	knockdown	NOUN
ajst-25583	43	9	of	of	ADP
ajst-25583	43	10	pps	pps	NOUN
ajst-25583	43	11	manipulators	manipulator	NOUN
ajst-25583	43	12	up	up	ADP
ajst-25583	43	13	to	to	ADP
ajst-25583	43	14	38	38	NUM
ajst-25583	43	15	kb	kb	NOUN
ajst-25583	43	16	long	long	ADV
ajst-25583	43	17	with	with	ADP
ajst-25583	43	18	80	80	NUM
ajst-25583	43	19	%	%	NOUN
ajst-25583	43	20	efficiency	efficiency	NOUN
ajst-25583	43	21	using	use	VERB
ajst-25583	43	22	crispr	crispr	ADJ
ajst-25583	43	23	-	-	PUNCT
ajst-25583	43	24	cas9	cas9	NOUN
ajst-25583	43	25	system	system	NOUN
ajst-25583	43	26	combined	combine	VERB
ajst-25583	43	27	with	with	ADP
ajst-25583	43	28	post	post	ADJ
ajst-25583	43	29	-	-	ADJ
ajst-25583	43	30	culture	culture	ADJ
ajst-25583	43	31	strategy	strategy	NOUN
ajst-25583	44	1	[	[	X
ajst-25583	44	2	33].liu	33].liu	NUM
ajst-25583	44	3	et	et	NOUN
ajst-25583	44	4	al	al	PROPN
ajst-25583	44	5	.	.	PROPN
ajst-25583	44	6	developed	develop	VERB
ajst-25583	44	7	a	a	DET
ajst-25583	44	8	crispr	crispr	NOUN
ajst-25583	44	9	/	/	SYM
ajst-25583	44	10	cas9n	cas9n	VERB
ajst-25583	44	11	-	-	PUNCT
ajst-25583	44	12	based	base	VERB
ajst-25583	44	13	bacillus	bacillus	NOUN
ajst-25583	44	14	subtilis	subtili	NOUN
ajst-25583	44	15	multiple	multiple	ADJ
ajst-25583	44	16	genome	genome	NOUN
ajst-25583	44	17	editing	editing	NOUN
ajst-25583	44	18	system	system	NOUN
ajst-25583	44	19	for	for	ADP
ajst-25583	44	20	iterative	iterative	NOUN
ajst-25583	44	21	editing	editing	NOUN
ajst-25583	44	22	of	of	ADP
ajst-25583	44	23	the	the	DET
ajst-25583	44	24	bacillus	bacillus	NOUN
ajst-25583	44	25	subtilis	subtili	NOUN
ajst-25583	44	26	genome	genome	NOUN
ajst-25583	44	27	.	.	PUNCT
ajst-25583	45	1	this	this	DET
ajst-25583	45	2	system	system	NOUN
ajst-25583	45	3	has	have	VERB
ajst-25583	45	4	an	an	DET
ajst-25583	45	5	efficiency	efficiency	NOUN
ajst-25583	45	6	of	of	ADP
ajst-25583	45	7	at	at	ADV
ajst-25583	45	8	least	least	ADV
ajst-25583	45	9	80	80	NUM
ajst-25583	45	10	%	%	NOUN
ajst-25583	45	11	for	for	ADP
ajst-25583	45	12	1	1	NUM
ajst-25583	45	13	-	-	SYM
ajst-25583	45	14	8	8	NUM
ajst-25583	45	15	kb	kb	PROPN
ajst-25583	45	16	gene	gene	NOUN
ajst-25583	45	17	deletions	deletion	NOUN
ajst-25583	45	18	,	,	PUNCT
ajst-25583	45	19	at	at	ADV
ajst-25583	45	20	least	least	ADV
ajst-25583	45	21	90	90	NUM
ajst-25583	45	22	%	%	NOUN
ajst-25583	45	23	for	for	ADP
ajst-25583	45	24	1	1	NUM
ajst-25583	45	25	-	-	SYM
ajst-25583	45	26	2	2	NUM
ajst-25583	45	27	kb	kb	PROPN
ajst-25583	45	28	gene	gene	NOUN
ajst-25583	45	29	insertions	insertion	NOUN
ajst-25583	45	30	,	,	PUNCT
ajst-25583	45	31	close	close	ADJ
ajst-25583	45	32	to	to	PART
ajst-25583	45	33	100	100	NUM
ajst-25583	45	34	%	%	NOUN
ajst-25583	45	35	for	for	ADP
ajst-25583	45	36	sentinel	sentinel	ADJ
ajst-25583	45	37	mutations	mutation	NOUN
ajst-25583	45	38	,	,	PUNCT
ajst-25583	45	39	23.6	23.6	NUM
ajst-25583	45	40	%	%	NOUN
ajst-25583	45	41	for	for	ADP
ajst-25583	45	42	large	large	ADJ
ajst-25583	45	43	segmental	segmental	ADJ
ajst-25583	45	44	dna	dna	NOUN
ajst-25583	45	45	deletions	deletion	NOUN
ajst-25583	45	46	,	,	PUNCT
ajst-25583	45	47	and	and	CCONJ
ajst-25583	45	48	close	close	ADJ
ajst-25583	45	49	to	to	PART
ajst-25583	45	50	50	50	NUM
ajst-25583	45	51	%	%	NOUN
ajst-25583	45	52	for	for	ADP
ajst-25583	45	53	three	three	NUM
ajst-25583	45	54	simultaneous	simultaneous	ADJ
ajst-25583	45	55	point	point	NOUN
ajst-25583	45	56	mutations	mutation	NOUN
ajst-25583	45	57	.	.	PUNCT
ajst-25583	46	1	the	the	DET
ajst-25583	46	2	efficiency	efficiency	NOUN
ajst-25583	46	3	of	of	ADP
ajst-25583	46	4	multiple	multiple	ADJ
ajst-25583	46	5	gene	gene	NOUN
ajst-25583	46	6	editing	editing	NOUN
ajst-25583	46	7	was	be	AUX
ajst-25583	46	8	further	far	ADV
ajst-25583	46	9	improved	improve	VERB
ajst-25583	46	10	by	by	ADP
ajst-25583	46	11	regulating	regulate	VERB
ajst-25583	46	12	nhej	nhej	ADJ
ajst-25583	46	13	mediated	mediate	VERB
ajst-25583	46	14	by	by	ADP
ajst-25583	46	15	the	the	DET
ajst-25583	46	16	ligd	ligd	NOUN
ajst-25583	46	17	gene	gene	NOUN
ajst-25583	46	18	,	,	PUNCT
ajst-25583	46	19	resulting	result	VERB
ajst-25583	46	20	in	in	ADP
ajst-25583	46	21	an	an	DET
ajst-25583	46	22	efficiency	efficiency	NOUN
ajst-25583	46	23	of	of	ADP
ajst-25583	46	24	about	about	ADV
ajst-25583	46	25	65	65	NUM
ajst-25583	46	26	%	%	NOUN
ajst-25583	46	27	for	for	ADP
ajst-25583	46	28	the	the	DET
ajst-25583	46	29	introduction	introduction	NOUN
ajst-25583	46	30	of	of	ADP
ajst-25583	46	31	3	3	NUM
ajst-25583	46	32	-	-	PUNCT
ajst-25583	46	33	point	point	NOUN
ajst-25583	46	34	mutations	mutation	NOUN
ajst-25583	46	35	on	on	ADP
ajst-25583	46	36	chromosomes	chromosome	NOUN
ajst-25583	46	37	.	.	PUNCT
ajst-25583	47	1	the	the	DET
ajst-25583	47	2	system	system	NOUN
ajst-25583	47	3	was	be	AUX
ajst-25583	47	4	also	also	ADV
ajst-25583	47	5	applied	apply	VERB
ajst-25583	47	6	to	to	ADP
ajst-25583	47	7	simultaneously	simultaneously	ADV
ajst-25583	47	8	fine	fine	ADJ
ajst-25583	47	9	-	-	PUNCT
ajst-25583	47	10	tune	tune	NOUN
ajst-25583	47	11	three	three	NUM
ajst-25583	47	12	genes	gene	NOUN
ajst-25583	47	13	in	in	ADP
ajst-25583	47	14	the	the	DET
ajst-25583	47	15	riboflavin	riboflavin	NOUN
ajst-25583	47	16	manipulator	manipulator	NOUN
ajst-25583	47	17	,	,	PUNCT
ajst-25583	47	18	and	and	CCONJ
ajst-25583	47	19	riboflavin	riboflavin	NOUN
ajst-25583	47	20	production	production	NOUN
ajst-25583	47	21	was	be	AUX
ajst-25583	47	22	significantly	significantly	ADV
ajst-25583	47	23	improved	improve	VERB
ajst-25583	47	24	in	in	ADP
ajst-25583	47	25	one	one	NUM
ajst-25583	47	26	cycle	cycle	NOUN
ajst-25583	48	1	[	[	X
ajst-25583	48	2	34	34	NUM
ajst-25583	48	3	]	]	PUNCT
ajst-25583	48	4	.	.	PUNCT
ajst-25583	49	1	3	3	X
ajst-25583	49	2	.	.	X
ajst-25583	49	3	summary	summary	NOUN
ajst-25583	49	4	and	and	CCONJ
ajst-25583	49	5	outlook	outlook	NOUN
ajst-25583	49	6	bacillus	bacillus	NOUN
ajst-25583	49	7	subtilis	subtili	NOUN
ajst-25583	49	8	,	,	PUNCT
ajst-25583	49	9	as	as	ADP
ajst-25583	49	10	a	a	DET
ajst-25583	49	11	gras	gra	NOUN
ajst-25583	49	12	strain	strain	NOUN
ajst-25583	49	13	,	,	PUNCT
ajst-25583	49	14	has	have	VERB
ajst-25583	49	15	a	a	DET
ajst-25583	49	16	huge	huge	ADJ
ajst-25583	49	17	application	application	NOUN
ajst-25583	49	18	market	market	NOUN
ajst-25583	49	19	and	and	CCONJ
ajst-25583	49	20	prospects	prospect	NOUN
ajst-25583	49	21	in	in	ADP
ajst-25583	49	22	industries	industry	NOUN
ajst-25583	49	23	such	such	ADJ
ajst-25583	49	24	as	as	ADP
ajst-25583	49	25	food	food	NOUN
ajst-25583	49	26	,	,	PUNCT
ajst-25583	49	27	medicine	medicine	NOUN
ajst-25583	49	28	,	,	PUNCT
ajst-25583	49	29	and	and	CCONJ
ajst-25583	49	30	breeding	breeding	NOUN
ajst-25583	49	31	.	.	PUNCT
ajst-25583	50	1	utilizing	utilize	VERB
ajst-25583	50	2	metabolic	metabolic	NOUN
ajst-25583	50	3	engineering	engineering	NOUN
ajst-25583	50	4	technology	technology	NOUN
ajst-25583	50	5	to	to	PART
ajst-25583	50	6	purposefully	purposefully	ADV
ajst-25583	50	7	modify	modify	VERB
ajst-25583	50	8	cells	cell	NOUN
ajst-25583	50	9	and	and	CCONJ
ajst-25583	50	10	construct	construct	VERB
ajst-25583	50	11	highly	highly	ADV
ajst-25583	50	12	efficient	efficient	ADJ
ajst-25583	50	13	chassis	chassis	NOUN
ajst-25583	50	14	cells	cell	NOUN
ajst-25583	50	15	is	be	AUX
ajst-25583	50	16	one	one	NUM
ajst-25583	50	17	of	of	ADP
ajst-25583	50	18	the	the	DET
ajst-25583	50	19	effective	effective	ADJ
ajst-25583	50	20	strategies	strategy	NOUN
ajst-25583	50	21	to	to	PART
ajst-25583	50	22	enhance	enhance	VERB
ajst-25583	50	23	its	its	PRON
ajst-25583	50	24	industrial	industrial	ADJ
ajst-25583	50	25	application	application	NOUN
ajst-25583	50	26	potential	potential	NOUN
ajst-25583	50	27	and	and	CCONJ
ajst-25583	50	28	scope	scope	NOUN
ajst-25583	50	29	.	.	PUNCT
ajst-25583	51	1	based	base	VERB
ajst-25583	51	2	on	on	ADP
ajst-25583	51	3	the	the	DET
ajst-25583	51	4	crispr	crispr	ADJ
ajst-25583	51	5	gene	gene	NOUN
ajst-25583	51	6	editing	edit	VERB
ajst-25583	51	7	system	system	NOUN
ajst-25583	51	8	,	,	PUNCT
ajst-25583	51	9	strategies	strategy	NOUN
ajst-25583	51	10	such	such	ADJ
ajst-25583	51	11	as	as	ADP
ajst-25583	51	12	promoter	promoter	NOUN
ajst-25583	51	13	engineering	engineering	NOUN
ajst-25583	51	14	,	,	PUNCT
ajst-25583	51	15	enzyme	enzyme	NOUN
ajst-25583	51	16	engineering	engineering	NOUN
ajst-25583	51	17	,	,	PUNCT
ajst-25583	51	18	cofactor	cofactor	NOUN
ajst-25583	51	19	engineering	engineering	NOUN
ajst-25583	51	20	,	,	PUNCT
ajst-25583	51	21	and	and	CCONJ
ajst-25583	51	22	dynamic	dynamic	ADJ
ajst-25583	51	23	regulation	regulation	NOUN
ajst-25583	51	24	can	can	AUX
ajst-25583	51	25	achieve	achieve	VERB
ajst-25583	51	26	a	a	DET
ajst-25583	51	27	relative	relative	ADJ
ajst-25583	51	28	unity	unity	NOUN
ajst-25583	51	29	of	of	ADP
ajst-25583	51	30	high	high	ADJ
ajst-25583	51	31	yield	yield	NOUN
ajst-25583	51	32	,	,	PUNCT
ajst-25583	51	33	high	high	ADJ
ajst-25583	51	34	productivity	productivity	NOUN
ajst-25583	51	35	,	,	PUNCT
ajst-25583	51	36	and	and	CCONJ
ajst-25583	51	37	high	high	ADJ
ajst-25583	51	38	production	production	NOUN
ajst-25583	51	39	intensity	intensity	NOUN
ajst-25583	51	40	of	of	ADP
ajst-25583	51	41	chassis	chassis	NOUN
ajst-25583	51	42	cells	cell	NOUN
ajst-25583	51	43	.	.	PUNCT
ajst-25583	52	1	however	however	ADV
ajst-25583	52	2	,	,	PUNCT
ajst-25583	52	3	the	the	DET
ajst-25583	52	4	current	current	ADJ
ajst-25583	52	5	design	design	NOUN
ajst-25583	52	6	and	and	CCONJ
ajst-25583	52	7	construction	construction	NOUN
ajst-25583	52	8	process	process	NOUN
ajst-25583	52	9	of	of	ADP
ajst-25583	52	10	chassis	chassis	NOUN
ajst-25583	52	11	cells	cell	NOUN
ajst-25583	52	12	is	be	AUX
ajst-25583	52	13	still	still	ADV
ajst-25583	52	14	rather	rather	ADV
ajst-25583	52	15	cumbersome	cumbersome	ADJ
ajst-25583	52	16	,	,	PUNCT
ajst-25583	52	17	and	and	CCONJ
ajst-25583	52	18	it	it	PRON
ajst-25583	52	19	is	be	AUX
ajst-25583	52	20	difficult	difficult	ADJ
ajst-25583	52	21	to	to	PART
ajst-25583	52	22	obtain	obtain	VERB
ajst-25583	52	23	universal	universal	ADJ
ajst-25583	52	24	metabolic	metabolic	NOUN
ajst-25583	52	25	rules	rule	NOUN
ajst-25583	52	26	after	after	ADP
ajst-25583	52	27	complex	complex	ADJ
ajst-25583	52	28	testing	testing	NOUN
ajst-25583	52	29	steps	step	NOUN
ajst-25583	52	30	.	.	PUNCT
ajst-25583	53	1	therefore	therefore	ADV
ajst-25583	53	2	,	,	PUNCT
ajst-25583	53	3	how	how	SCONJ
ajst-25583	53	4	to	to	PART
ajst-25583	53	5	improve	improve	VERB
ajst-25583	53	6	the	the	DET
ajst-25583	53	7	efficiency	efficiency	NOUN
ajst-25583	53	8	of	of	ADP
ajst-25583	53	9	the	the	DET
ajst-25583	53	10	design	design	NOUN
ajst-25583	53	11	-	-	PUNCT
ajst-25583	53	12	buildtest	buildtest	NOUN
ajst-25583	53	13	-	-	PUNCT
ajst-25583	53	14	learn	learn	NOUN
ajst-25583	53	15	cycle	cycle	NOUN
ajst-25583	53	16	is	be	AUX
ajst-25583	53	17	one	one	NUM
ajst-25583	53	18	of	of	ADP
ajst-25583	53	19	the	the	DET
ajst-25583	53	20	problems	problem	NOUN
ajst-25583	53	21	awaiting	await	VERB
ajst-25583	53	22	a	a	DET
ajst-25583	53	23	solution	solution	NOUN
ajst-25583	53	24	.	.	PUNCT
ajst-25583	54	1	the	the	DET
ajst-25583	54	2	intersection	intersection	NOUN
ajst-25583	54	3	and	and	CCONJ
ajst-25583	54	4	combination	combination	NOUN
ajst-25583	54	5	of	of	ADP
ajst-25583	54	6	synthetic	synthetic	ADJ
ajst-25583	54	7	biology	biology	NOUN
ajst-25583	54	8	,	,	PUNCT
ajst-25583	54	9	systems	system	NOUN
ajst-25583	54	10	biology	biology	NOUN
ajst-25583	54	11	,	,	PUNCT
ajst-25583	54	12	and	and	CCONJ
ajst-25583	54	13	multiple	multiple	ADJ
ajst-25583	54	14	disciplines	discipline	NOUN
ajst-25583	54	15	will	will	AUX
ajst-25583	54	16	become	become	VERB
ajst-25583	54	17	a	a	DET
ajst-25583	54	18	breakthrough	breakthrough	NOUN
ajst-25583	54	19	to	to	PART
ajst-25583	54	20	solve	solve	VERB
ajst-25583	54	21	this	this	DET
ajst-25583	54	22	problem	problem	NOUN
ajst-25583	54	23	.	.	PUNCT
ajst-25583	55	1	on	on	ADP
ajst-25583	55	2	the	the	DET
ajst-25583	55	3	one	one	NUM
ajst-25583	55	4	hand	hand	NOUN
ajst-25583	55	5	,	,	PUNCT
ajst-25583	55	6	the	the	DET
ajst-25583	55	7	intersection	intersection	NOUN
ajst-25583	55	8	and	and	CCONJ
ajst-25583	55	9	combination	combination	NOUN
ajst-25583	55	10	of	of	ADP
ajst-25583	55	11	artificial	artificial	ADJ
ajst-25583	55	12	intelligence	intelligence	NOUN
ajst-25583	55	13	,	,	PUNCT
ajst-25583	55	14	machine	machine	NOUN
ajst-25583	55	15	learning	learning	NOUN
ajst-25583	55	16	,	,	PUNCT
ajst-25583	55	17	and	and	CCONJ
ajst-25583	55	18	multi	multi	ADJ
ajst-25583	55	19	-	-	ADJ
ajst-25583	55	20	omics	omics	ADJ
ajst-25583	55	21	disciplines	discipline	NOUN
ajst-25583	55	22	provides	provide	VERB
ajst-25583	55	23	guarantees	guarantee	NOUN
ajst-25583	55	24	for	for	ADP
ajst-25583	55	25	annotating	annotate	VERB
ajst-25583	55	26	gene	gene	NOUN
ajst-25583	55	27	functions	function	NOUN
ajst-25583	55	28	,	,	PUNCT
ajst-25583	55	29	analyzing	analyze	VERB
ajst-25583	55	30	regulatory	regulatory	ADJ
ajst-25583	55	31	mechanisms	mechanism	NOUN
ajst-25583	55	32	,	,	PUNCT
ajst-25583	55	33	and	and	CCONJ
ajst-25583	55	34	revealing	reveal	VERB
ajst-25583	55	35	the	the	DET
ajst-25583	55	36	laws	law	NOUN
ajst-25583	55	37	of	of	ADP
ajst-25583	55	38	life	life	NOUN
ajst-25583	55	39	.	.	PUNCT
ajst-25583	56	1	on	on	ADP
ajst-25583	56	2	the	the	DET
ajst-25583	56	3	other	other	ADJ
ajst-25583	56	4	hand	hand	NOUN
ajst-25583	56	5	,	,	PUNCT
ajst-25583	56	6	directed	direct	VERB
ajst-25583	56	7	evolution	evolution	NOUN
ajst-25583	56	8	,	,	PUNCT
ajst-25583	56	9	computational	computational	ADJ
ajst-25583	56	10	tools	tool	NOUN
ajst-25583	56	11	,	,	PUNCT
ajst-25583	56	12	or	or	CCONJ
ajst-25583	56	13	de	de	ADP
ajst-25583	56	14	novo	novo	NOUN
ajst-25583	56	15	synthesis	synthesis	NOUN
ajst-25583	56	16	of	of	ADP
ajst-25583	56	17	non	non	ADJ
ajst-25583	56	18	-	-	ADJ
ajst-25583	56	19	natural	natural	ADJ
ajst-25583	56	20	enzymes	enzyme	NOUN
ajst-25583	56	21	or	or	CCONJ
ajst-25583	56	22	regulatory	regulatory	ADJ
ajst-25583	56	23	proteins	protein	NOUN
ajst-25583	56	24	also	also	ADV
ajst-25583	56	25	bring	bring	VERB
ajst-25583	56	26	new	new	ADJ
ajst-25583	56	27	opportunities	opportunity	NOUN
ajst-25583	56	28	for	for	ADP
ajst-25583	56	29	constructing	construct	VERB
ajst-25583	56	30	high	high	ADJ
ajst-25583	56	31	-	-	PUNCT
ajst-25583	56	32	version	version	NOUN
ajst-25583	56	33	chassis	chassis	NOUN
ajst-25583	56	34	cells	cell	NOUN
ajst-25583	56	35	.	.	PUNCT
ajst-25583	57	1	references	reference	NOUN
ajst-25583	57	2	[	[	X
ajst-25583	57	3	1	1	NUM
ajst-25583	57	4	]	]	PUNCT
ajst-25583	57	5	m.	m.	NOUN
ajst-25583	57	6	maki	maki	PROPN
ajst-25583	57	7	,	,	PUNCT
ajst-25583	57	8	k.	k.	PROPN
ajst-25583	57	9	t.	t.	PROPN
ajst-25583	57	10	leung	leung	PROPN
ajst-25583	57	11	and	and	CCONJ
ajst-25583	57	12	w.	w.	PROPN
ajst-25583	57	13	s.	s.	PROPN
ajst-25583	57	14	qin	qin	PROPN
ajst-25583	57	15	:	:	PUNCT
ajst-25583	57	16	the	the	DET
ajst-25583	57	17	prospects	prospect	NOUN
ajst-25583	57	18	of	of	ADP
ajst-25583	57	19	cellulase	cellulase	NOUN
ajst-25583	57	20	-	-	PUNCT
ajst-25583	57	21	producing	produce	VERB
ajst-25583	57	22	bacteria	bacteria	NOUN
ajst-25583	57	23	for	for	ADP
ajst-25583	57	24	the	the	DET
ajst-25583	57	25	bioconversion	bioconversion	NOUN
ajst-25583	57	26	of	of	ADP
ajst-25583	57	27	lignocellulosic	lignocellulosic	ADJ
ajst-25583	57	28	biomass	biomass	NOUN
ajst-25583	57	29	,	,	PUNCT
ajst-25583	57	30	international	international	ADJ
ajst-25583	57	31	journal	journal	NOUN
ajst-25583	57	32	of	of	ADP
ajst-25583	57	33	biological	biological	ADJ
ajst-25583	57	34	sciences	science	NOUN
ajst-25583	57	35	,	,	PUNCT
ajst-25583	57	36	vol	vol	NOUN
ajst-25583	57	37	.	.	PROPN
ajst-25583	57	38	5	5	NUM
ajst-25583	57	39	(	(	PUNCT
ajst-25583	57	40	2009	2009	NUM
ajst-25583	57	41	)	)	PUNCT
ajst-25583	57	42	no.5	no.5	PROPN
ajst-25583	57	43	,	,	PUNCT
ajst-25583	57	44	p.500	p.500	NOUN
ajst-25583	57	45	-	-	PUNCT
ajst-25583	57	46	516	516	NUM
ajst-25583	57	47	.	.	PUNCT
ajst-25583	58	1	[	[	X
ajst-25583	58	2	2	2	NUM
ajst-25583	58	3	]	]	PUNCT
ajst-25583	58	4	m.	m.	NOUN
ajst-25583	58	5	s.	s.	PROPN
ajst-25583	58	6	ou	ou	PROPN
ajst-25583	58	7	,	,	PUNCT
ajst-25583	58	8	n.	n.	PROPN
ajst-25583	58	9	mohammed	mohammed	PROPN
ajst-25583	58	10	,	,	PUNCT
ajst-25583	58	11	l.	l.	PROPN
ajst-25583	58	12	o.	o.	PROPN
ajst-25583	58	13	ingram	ingram	PROPN
ajst-25583	58	14	and	and	CCONJ
ajst-25583	58	15	k.	k.	PROPN
ajst-25583	58	16	t.	t.	PROPN
ajst-25583	58	17	shanmugam	shanmugam	PROPN
ajst-25583	58	18	:	:	PUNCT
ajst-25583	58	19	thermophilic	thermophilic	ADJ
ajst-25583	58	20	bacillus	bacillus	NOUN
ajst-25583	58	21	coagulans	coagulan	NOUN
ajst-25583	58	22	requires	require	VERB
ajst-25583	58	23	less	less	ADJ
ajst-25583	58	24	cellulases	cellulase	NOUN
ajst-25583	58	25	for	for	ADP
ajst-25583	58	26	simultaneous	simultaneous	ADJ
ajst-25583	58	27	saccharification	saccharification	NOUN
ajst-25583	58	28	and	and	CCONJ
ajst-25583	58	29	fermentation	fermentation	NOUN
ajst-25583	58	30	of	of	ADP
ajst-25583	58	31	cellulose	cellulose	NOUN
ajst-25583	58	32	to	to	ADP
ajst-25583	58	33	products	product	NOUN
ajst-25583	58	34	than	than	ADP
ajst-25583	58	35	mesophilic	mesophilic	ADJ
ajst-25583	58	36	microbial	microbial	ADJ
ajst-25583	58	37	biocatalysts	biocatalyst	NOUN
ajst-25583	58	38	,	,	PUNCT
ajst-25583	58	39	applied	apply	VERB
ajst-25583	58	40	biochemistry	biochemistry	NOUN
ajst-25583	58	41	and	and	CCONJ
ajst-25583	58	42	biotechnology	biotechnology	NOUN
ajst-25583	58	43	,	,	PUNCT
ajst-25583	58	44	vol	vol	NOUN
ajst-25583	58	45	.	.	PROPN
ajst-25583	59	1	155	155	NUM
ajst-25583	59	2	(	(	PUNCT
ajst-25583	59	3	2009	2009	NUM
ajst-25583	59	4	)	)	PUNCT
ajst-25583	59	5	no.1	no.1	NOUN
ajst-25583	59	6	-	-	SYM
ajst-25583	59	7	3	3	NUM
ajst-25583	59	8	,	,	PUNCT
ajst-25583	59	9	p.379	p.379	NOUN
ajst-25583	59	10	-	-	SYM
ajst-25583	59	11	385	385	NUM
ajst-25583	59	12	.	.	PUNCT
ajst-25583	60	1	[	[	X
ajst-25583	60	2	3	3	X
ajst-25583	60	3	]	]	X
ajst-25583	60	4	c.	c.	PROPN
ajst-25583	60	5	michon	michon	PROPN
ajst-25583	60	6	,	,	PUNCT
ajst-25583	60	7	c.	c.	PROPN
ajst-25583	60	8	m.	m.	PROPN
ajst-25583	60	9	kang	kang	PROPN
ajst-25583	60	10	,	,	PUNCT
ajst-25583	60	11	s.	s.	PROPN
ajst-25583	60	12	karpenko	karpenko	PROPN
ajst-25583	60	13	,	,	PUNCT
ajst-25583	60	14	k.	k.	PROPN
ajst-25583	60	15	tanaka	tanaka	PROPN
ajst-25583	60	16	,	,	PUNCT
ajst-25583	60	17	s.	s.	PROPN
ajst-25583	60	18	ishikawa	ishikawa	PROPN
ajst-25583	60	19	and	and	CCONJ
ajst-25583	60	20	k.	k.	PROPN
ajst-25583	60	21	yoshida	yoshida	PROPN
ajst-25583	60	22	:	:	PUNCT
ajst-25583	60	23	a	a	DET
ajst-25583	60	24	bacterial	bacterial	ADJ
ajst-25583	60	25	cell	cell	NOUN
ajst-25583	60	26	factory	factory	NOUN
ajst-25583	60	27	converting	convert	VERB
ajst-25583	60	28	glucose	glucose	NOUN
ajst-25583	60	29	into	into	ADP
ajst-25583	60	30	scyllo	scyllo	NOUN
ajst-25583	60	31	-	-	PUNCT
ajst-25583	60	32	inositol	inositol	NOUN
ajst-25583	60	33	,	,	PUNCT
ajst-25583	60	34	a	a	DET
ajst-25583	60	35	therapeutic	therapeutic	ADJ
ajst-25583	60	36	agent	agent	NOUN
ajst-25583	60	37	for	for	ADP
ajst-25583	60	38	alzheimer	alzheimer	PROPN
ajst-25583	60	39	's	's	PART
ajst-25583	60	40	disease	disease	NOUN
ajst-25583	60	41	,	,	PUNCT
ajst-25583	60	42	communications	communication	NOUN
ajst-25583	60	43	biology	biology	NOUN
ajst-25583	60	44	,	,	PUNCT
ajst-25583	60	45	vol	vol	NOUN
ajst-25583	60	46	.	.	NOUN
ajst-25583	60	47	3	3	NUM
ajst-25583	60	48	(	(	PUNCT
ajst-25583	60	49	2020	2020	NUM
ajst-25583	60	50	)	)	PUNCT
ajst-25583	60	51	no.1	no.1	NOUN
ajst-25583	60	52	,	,	PUNCT
ajst-25583	60	53	p.93	p.93	NOUN
ajst-25583	60	54	-	-	X
ajst-25583	60	55	99	99	NUM
ajst-25583	60	56	.	.	PUNCT
ajst-25583	61	1	[	[	X
ajst-25583	61	2	4	4	X
ajst-25583	61	3	]	]	PUNCT
ajst-25583	61	4	k.	k.	PROPN
ajst-25583	61	5	tanaka	tanaka	PROPN
ajst-25583	61	6	,	,	PUNCT
ajst-25583	61	7	a.	a.	NOUN
ajst-25583	61	8	natsume	natsume	PROPN
ajst-25583	61	9	,	,	PUNCT
ajst-25583	61	10	s.	s.	PROPN
ajst-25583	61	11	ishikawa	ishikawa	PROPN
ajst-25583	61	12	,	,	PUNCT
ajst-25583	61	13	s.	s.	PROPN
ajst-25583	61	14	takenaka	takenaka	PROPN
ajst-25583	61	15	and	and	CCONJ
ajst-25583	61	16	k.	k.	PROPN
ajst-25583	61	17	yoshida	yoshida	PROPN
ajst-25583	61	18	:	:	PUNCT
ajst-25583	61	19	a	a	DET
ajst-25583	61	20	new	new	ADJ
ajst-25583	61	21	-	-	PUNCT
ajst-25583	61	22	generation	generation	NOUN
ajst-25583	61	23	of	of	ADP
ajst-25583	61	24	bacillus	bacillus	NOUN
ajst-25583	61	25	subtilis	subtili	NOUN
ajst-25583	61	26	cell	cell	NOUN
ajst-25583	61	27	factory	factory	NOUN
ajst-25583	61	28	for	for	ADP
ajst-25583	61	29	further	further	ADJ
ajst-25583	61	30	elevated	elevate	VERB
ajst-25583	61	31	scyllo	scyllo	NOUN
ajst-25583	61	32	-	-	PUNCT
ajst-25583	61	33	inositol	inositol	NOUN
ajst-25583	61	34	production	production	NOUN
ajst-25583	61	35	,	,	PUNCT
ajst-25583	61	36	microbial	microbial	ADJ
ajst-25583	61	37	cell	cell	NOUN
ajst-25583	61	38	factories	factory	NOUN
ajst-25583	61	39	,	,	PUNCT
ajst-25583	61	40	vol	vol	NOUN
ajst-25583	61	41	.	.	PROPN
ajst-25583	62	1	16	16	NUM
ajst-25583	62	2	(	(	PUNCT
ajst-25583	62	3	2017	2017	NUM
ajst-25583	62	4	)	)	PUNCT
ajst-25583	62	5	,	,	PUNCT
ajst-25583	62	6	p.67	p.67	PROPN
ajst-25583	62	7	-	-	NOUN
ajst-25583	62	8	74	74	NUM
ajst-25583	62	9	.	.	PUNCT
ajst-25583	63	1	[	[	X
ajst-25583	63	2	5	5	X
ajst-25583	63	3	]	]	PUNCT
ajst-25583	63	4	l.	l.	PROPN
ajst-25583	63	5	liu	liu	PROPN
ajst-25583	63	6	,	,	PUNCT
ajst-25583	63	7	y.	y.	PROPN
ajst-25583	63	8	f.	f.	PROPN
ajst-25583	63	9	liu	liu	PROPN
ajst-25583	63	10	,	,	PUNCT
ajst-25583	63	11	h.	h.	PROPN
ajst-25583	63	12	d.	d.	PROPN
ajst-25583	63	13	shin	shin	PROPN
ajst-25583	63	14	,	,	PUNCT
ajst-25583	63	15	r.	r.	PROPN
ajst-25583	63	16	r.	r.	PROPN
ajst-25583	63	17	chen	chen	PROPN
ajst-25583	63	18	,	,	PUNCT
ajst-25583	63	19	n.	n.	PROPN
ajst-25583	63	20	s.	s.	PROPN
ajst-25583	63	21	wang	wang	PROPN
ajst-25583	63	22	,	,	PUNCT
ajst-25583	63	23	j.	j.	PROPN
ajst-25583	63	24	h.	h.	PROPN
ajst-25583	63	25	li	li	PROPN
ajst-25583	63	26	,	,	PUNCT
ajst-25583	63	27	g.	g.	PROPN
ajst-25583	63	28	c.	c.	PROPN
ajst-25583	63	29	du	du	PROPN
ajst-25583	63	30	and	and	CCONJ
ajst-25583	63	31	j.	j.	PROPN
ajst-25583	63	32	chen	chen	PROPN
ajst-25583	63	33	:	:	PUNCT
ajst-25583	63	34	developing	develop	VERB
ajst-25583	63	35	bacillus	bacillus	NOUN
ajst-25583	63	36	spp	spp	NOUN
ajst-25583	63	37	.	.	PUNCT
ajst-25583	64	1	as	as	ADP
ajst-25583	64	2	a	a	DET
ajst-25583	64	3	cell	cell	NOUN
ajst-25583	64	4	factory	factory	NOUN
ajst-25583	64	5	for	for	ADP
ajst-25583	64	6	production	production	NOUN
ajst-25583	64	7	of	of	ADP
ajst-25583	64	8	microbial	microbial	ADJ
ajst-25583	64	9	enzymes	enzyme	NOUN
ajst-25583	64	10	and	and	CCONJ
ajst-25583	64	11	industrially	industrially	ADV
ajst-25583	64	12	important	important	ADJ
ajst-25583	64	13	biochemicals	biochemical	NOUN
ajst-25583	64	14	in	in	ADP
ajst-25583	64	15	the	the	DET
ajst-25583	64	16	context	context	NOUN
ajst-25583	64	17	of	of	ADP
ajst-25583	64	18	systems	system	NOUN
ajst-25583	64	19	and	and	CCONJ
ajst-25583	64	20	synthetic	synthetic	ADJ
ajst-25583	64	21	biology	biology	NOUN
ajst-25583	64	22	,	,	PUNCT
ajst-25583	64	23	applied	apply	VERB
ajst-25583	64	24	microbiology	microbiology	NOUN
ajst-25583	64	25	and	and	CCONJ
ajst-25583	64	26	biotechnology	biotechnology	NOUN
ajst-25583	64	27	,	,	PUNCT
ajst-25583	64	28	vol	vol	NOUN
ajst-25583	64	29	.	.	PROPN
ajst-25583	65	1	97	97	NUM
ajst-25583	65	2	(	(	PUNCT
ajst-25583	65	3	2013	2013	NUM
ajst-25583	65	4	)	)	PUNCT
ajst-25583	65	5	no.14	no.14	PROPN
ajst-25583	65	6	,	,	PUNCT
ajst-25583	65	7	p.6113	p.6113	NOUN
ajst-25583	65	8	-	-	PUNCT
ajst-25583	65	9	6127	6127	NUM
ajst-25583	65	10	.	.	PUNCT
ajst-25583	66	1	[	[	X
ajst-25583	66	2	6	6	NUM
ajst-25583	66	3	]	]	PUNCT
ajst-25583	66	4	z.	z.	PROPN
ajst-25583	66	5	guan	guan	PROPN
ajst-25583	66	6	,	,	PUNCT
ajst-25583	66	7	d.	d.	PROPN
ajst-25583	66	8	xue	xue	PROPN
ajst-25583	66	9	,	,	PUNCT
ajst-25583	66	10	abdallah	abdallah	PROPN
ajst-25583	66	11	,	,	PUNCT
ajst-25583	66	12	ii	ii	PROPN
ajst-25583	66	13	,	,	PUNCT
ajst-25583	66	14	l.	l.	PROPN
ajst-25583	66	15	dijkshoorn	dijkshoorn	PROPN
ajst-25583	66	16	,	,	PUNCT
ajst-25583	66	17	r.	r.	PROPN
ajst-25583	66	18	setroikromo	setroikromo	PROPN
ajst-25583	66	19	,	,	PUNCT
ajst-25583	66	20	g.	g.	PROPN
ajst-25583	66	21	y.	y.	PROPN
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ajst-25583	66	25	j.	j.	PROPN
ajst-25583	66	26	quax	quax	PROPN
ajst-25583	66	27	:	:	PUNCT
ajst-25583	66	28	metabolic	metabolic	PROPN
ajst-25583	66	29	engineering	engineering	NOUN
ajst-25583	66	30	of	of	ADP
ajst-25583	66	31	bacillus	bacillus	NOUN
ajst-25583	66	32	subtilis	subtili	NOUN
ajst-25583	66	33	for	for	ADP
ajst-25583	66	34	terpenoid	terpenoid	NOUN
ajst-25583	66	35	production	production	NOUN
ajst-25583	66	36	,	,	PUNCT
ajst-25583	66	37	applied	apply	VERB
ajst-25583	66	38	microbiology	microbiology	NOUN
ajst-25583	66	39	and	and	CCONJ
ajst-25583	66	40	biotechnology	biotechnology	NOUN
ajst-25583	66	41	,	,	PUNCT
ajst-25583	66	42	vol	vol	NOUN
ajst-25583	66	43	.	.	PROPN
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ajst-25583	67	2	(	(	PUNCT
ajst-25583	67	3	2015	2015	NUM
ajst-25583	67	4	)	)	PUNCT
ajst-25583	67	5	no.22	no.22	NOUN
ajst-25583	67	6	,	,	PUNCT
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ajst-25583	67	8	-	-	PUNCT
ajst-25583	67	9	9406	9406	NUM
ajst-25583	67	10	.	.	PUNCT
ajst-25583	68	1	[	[	X
ajst-25583	68	2	7	7	X
ajst-25583	68	3	]	]	X
ajst-25583	68	4	y.	y.	PROPN
ajst-25583	68	5	f.	f.	PROPN
ajst-25583	68	6	song	song	PROPN
ajst-25583	68	7	,	,	PUNCT
ajst-25583	68	8	z.	z.	PROPN
ajst-25583	68	9	guan	guan	PROPN
ajst-25583	68	10	,	,	PUNCT
ajst-25583	68	11	r.	r.	PROPN
ajst-25583	68	12	van	van	PROPN
ajst-25583	68	13	merkerk	merkerk	PROPN
ajst-25583	68	14	,	,	PUNCT
ajst-25583	68	15	h.	h.	PROPN
ajst-25583	68	16	pramastya	pramastya	PROPN
ajst-25583	68	17	,	,	PUNCT
ajst-25583	68	18	abdallah	abdallah	PROPN
ajst-25583	68	19	,	,	PUNCT
ajst-25583	68	20	ii	ii	PROPN
ajst-25583	68	21	,	,	PUNCT
ajst-25583	68	22	r.	r.	PROPN
ajst-25583	68	23	setroikromo	setroikromo	PROPN
ajst-25583	68	24	and	and	CCONJ
ajst-25583	68	25	w.	w.	PROPN
ajst-25583	68	26	j.	j.	PROPN
ajst-25583	68	27	quax	quax	PROPN
ajst-25583	68	28	:	:	PUNCT
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ajst-25583	68	30	of	of	ADP
ajst-25583	68	31	squalene	squalene	NOUN
ajst-25583	68	32	in	in	ADP
ajst-25583	68	33	bacillus	bacillus	NOUN
ajst-25583	68	34	subtilis	subtili	NOUN
ajst-25583	68	35	by	by	ADP
ajst-25583	68	36	squalene	squalene	NOUN
ajst-25583	68	37	synthase	synthase	NOUN
ajst-25583	68	38	screening	screening	NOUN
ajst-25583	68	39	and	and	CCONJ
ajst-25583	68	40	metabolic	metabolic	NOUN
ajst-25583	68	41	engineering	engineering	NOUN
ajst-25583	68	42	,	,	PUNCT
ajst-25583	68	43	journal	journal	NOUN
ajst-25583	68	44	of	of	ADP
ajst-25583	68	45	agricultural	agricultural	ADJ
ajst-25583	68	46	and	and	CCONJ
ajst-25583	68	47	food	food	NOUN
ajst-25583	68	48	chemistry	chemistry	NOUN
ajst-25583	68	49	,	,	PUNCT
ajst-25583	68	50	vol	vol	NOUN
ajst-25583	68	51	.	.	PROPN
ajst-25583	69	1	68	68	NUM
ajst-25583	69	2	(	(	PUNCT
ajst-25583	69	3	2015	2015	NUM
ajst-25583	69	4	)	)	PUNCT
ajst-25583	69	5	no.15	no.15	NOUN
ajst-25583	69	6	,	,	PUNCT
ajst-25583	69	7	p.4447	p.4447	NOUN
ajst-25583	69	8	-	-	PUNCT
ajst-25583	69	9	4455	4455	NUM
ajst-25583	69	10	.	.	PUNCT
ajst-25583	70	1	[	[	X
ajst-25583	70	2	8	8	NUM
ajst-25583	70	3	]	]	PUNCT
ajst-25583	70	4	k.	k.	PROPN
ajst-25583	70	5	zhou	zhou	PROPN
ajst-25583	70	6	,	,	PUNCT
ajst-25583	70	7	r.	r.	PROPN
ajst-25583	70	8	y.	y.	PROPN
ajst-25583	70	9	zou	zou	PROPN
ajst-25583	70	10	,	,	PUNCT
ajst-25583	70	11	c.	c.	PROPN
ajst-25583	70	12	q.	q.	PROPN
ajst-25583	70	13	zhang	zhang	PROPN
ajst-25583	70	14	,	,	PUNCT
ajst-25583	70	15	g.	g.	PROPN
ajst-25583	70	16	stephanopoulos	stephanopoulos	PROPN
ajst-25583	70	17	and	and	CCONJ
ajst-25583	70	18	h.	h.	PROPN
ajst-25583	70	19	p.	p.	PROPN
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ajst-25583	70	21	:	:	PUNCT
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ajst-25583	70	23	of	of	ADP
ajst-25583	70	24	amorphadiene	amorphadiene	ADJ
ajst-25583	70	25	synthesis	synthesis	NOUN
ajst-25583	70	26	in	in	ADP
ajst-25583	70	27	bacillus	bacillus	NOUN
ajst-25583	70	28	subtilis	subtili	NOUN
ajst-25583	70	29	via	via	ADP
ajst-25583	70	30	transcriptional	transcriptional	ADJ
ajst-25583	70	31	,	,	PUNCT
ajst-25583	70	32	translational	translational	ADJ
ajst-25583	70	33	,	,	PUNCT
ajst-25583	70	34	and	and	CCONJ
ajst-25583	70	35	media	medium	NOUN
ajst-25583	70	36	modulation	modulation	NOUN
ajst-25583	70	37	,	,	PUNCT
ajst-25583	70	38	biotechnology	biotechnology	NOUN
ajst-25583	70	39	and	and	CCONJ
ajst-25583	70	40	bioengineering	bioengineering	NOUN
ajst-25583	70	41	,	,	PUNCT
ajst-25583	70	42	vol	vol	NOUN
ajst-25583	70	43	.	.	PUNCT
ajst-25583	71	1	110	110	NUM
ajst-25583	71	2	(	(	PUNCT
ajst-25583	71	3	2015	2015	NUM
ajst-25583	71	4	)	)	PUNCT
ajst-25583	72	1	no.9	no.9	PROPN
ajst-25583	72	2	,	,	PUNCT
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ajst-25583	72	4	-	-	PUNCT
ajst-25583	72	5	2561	2561	NUM
ajst-25583	72	6	.	.	PUNCT
ajst-25583	73	1	[	[	X
ajst-25583	73	2	9	9	X
ajst-25583	73	3	]	]	X
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ajst-25583	73	5	f.	f.	PROPN
ajst-25583	73	6	liu	liu	PROPN
ajst-25583	73	7	,	,	PUNCT
ajst-25583	73	8	h.	h.	PROPN
ajst-25583	73	9	link	link	PROPN
ajst-25583	73	10	,	,	PUNCT
ajst-25583	73	11	l.	l.	PROPN
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ajst-25583	73	13	,	,	PUNCT
ajst-25583	73	14	g.	g.	PROPN
ajst-25583	73	15	c.	c.	PROPN
ajst-25583	73	16	du	du	PROPN
ajst-25583	73	17	,	,	PUNCT
ajst-25583	73	18	j.	j.	PROPN
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ajst-25583	73	26	pathway	pathway	NOUN
ajst-25583	73	27	analysis	analysis	NOUN
ajst-25583	73	28	approach	approach	NOUN
ajst-25583	73	29	reveals	reveal	VERB
ajst-25583	73	30	a	a	DET
ajst-25583	73	31	limiting	limit	VERB
ajst-25583	73	32	futile	futile	ADJ
ajst-25583	73	33	cycle	cycle	NOUN
ajst-25583	73	34	in	in	ADP
ajst-25583	73	35	n	n	CCONJ
ajst-25583	73	36	-	-	PUNCT
ajst-25583	73	37	acetylglucosamine	acetylglucosamine	NOUN
ajst-25583	73	38	overproducing	overproduce	VERB
ajst-25583	73	39	bacillus	bacillus	NOUN
ajst-25583	73	40	subtilis	subtili	NOUN
ajst-25583	73	41	,	,	PUNCT
ajst-25583	73	42	nature	nature	NOUN
ajst-25583	73	43	communications	communication	NOUN
ajst-25583	73	44	,	,	PUNCT
ajst-25583	73	45	vol	vol	NOUN
ajst-25583	73	46	.	.	PROPN
ajst-25583	73	47	7	7	NUM
ajst-25583	73	48	(	(	PUNCT
ajst-25583	73	49	2016	2016	NUM
ajst-25583	73	50	)	)	PUNCT
ajst-25583	73	51	,	,	PUNCT
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ajst-25583	73	53	-	-	PUNCT
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ajst-25583	73	55	.	.	PUNCT
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ajst-25583	74	2	10	10	NUM
ajst-25583	74	3	]	]	PUNCT
ajst-25583	74	4	t.	t.	PROPN
ajst-25583	74	5	f.	f.	PROPN
ajst-25583	74	6	niu	niu	PROPN
ajst-25583	74	7	,	,	PUNCT
ajst-25583	74	8	y.	y.	PROPN
ajst-25583	74	9	f.	f.	PROPN
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ajst-25583	74	11	,	,	PUNCT
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ajst-25583	74	13	h.	h.	PROPN
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ajst-25583	74	17	koffas	koffas	NOUN
ajst-25583	74	18	,	,	PUNCT
ajst-25583	74	19	g.	g.	PROPN
ajst-25583	74	20	c.	c.	PROPN
ajst-25583	74	21	du	du	PROPN
ajst-25583	74	22	,	,	PUNCT
ajst-25583	74	23	h.	h.	PROPN
ajst-25583	74	24	s.	s.	PROPN
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ajst-25583	74	26	and	and	CCONJ
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ajst-25583	74	28	liu	liu	PROPN
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ajst-25583	74	31	a	a	DET
ajst-25583	74	32	glucosamine-6	glucosamine-6	PROPN
ajst-25583	74	33	-	-	PUNCT
ajst-25583	74	34	phosphate	phosphate	ADJ
ajst-25583	74	35	responsive	responsive	ADJ
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ajst-25583	74	37	ribozyme	ribozyme	ADJ
ajst-25583	74	38	switch	switch	NOUN
ajst-25583	74	39	enables	enable	VERB
ajst-25583	74	40	dynamic	dynamic	ADJ
ajst-25583	74	41	control	control	NOUN
ajst-25583	74	42	of	of	ADP
ajst-25583	74	43	metabolic	metabolic	NOUN
ajst-25583	74	44	flux	flux	NOUN
ajst-25583	74	45	in	in	ADP
ajst-25583	74	46	bacillus	bacillus	NOUN
ajst-25583	74	47	subtilis	subtili	NOUN
ajst-25583	74	48	for	for	ADP
ajst-25583	74	49	overproduction	overproduction	NOUN
ajst-25583	74	50	of	of	ADP
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ajst-25583	74	52	,	,	PUNCT
ajst-25583	74	53	acs	acs	PROPN
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ajst-25583	74	56	,	,	PUNCT
ajst-25583	74	57	vol	vol	NOUN
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ajst-25583	74	59	7	7	NUM
ajst-25583	74	60	(	(	PUNCT
ajst-25583	74	61	2018	2018	NUM
ajst-25583	74	62	)	)	PUNCT
ajst-25583	74	63	no.10	no.10	ADJ
ajst-25583	74	64	,	,	PUNCT
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ajst-25583	74	66	-	-	NOUN
ajst-25583	74	67	2435	2435	NUM
ajst-25583	74	68	.	.	PUNCT
ajst-25583	75	1	[	[	X
ajst-25583	75	2	11	11	NUM
ajst-25583	75	3	]	]	X
ajst-25583	75	4	y.	y.	PROPN
ajst-25583	75	5	f.	f.	PROPN
ajst-25583	75	6	liu	liu	PROPN
ajst-25583	75	7	,	,	PUNCT
ajst-25583	75	8	l.	l.	PROPN
ajst-25583	75	9	liu	liu	PROPN
ajst-25583	75	10	,	,	PUNCT
ajst-25583	75	11	h.	h.	PROPN
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ajst-25583	75	14	,	,	PUNCT
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ajst-25583	75	16	r.	r.	PROPN
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ajst-25583	75	20	h.	h.	PROPN
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ajst-25583	75	24	c.	c.	PROPN
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ajst-25583	75	31	engineering	engineering	NOUN
ajst-25583	75	32	of	of	ADP
ajst-25583	75	33	bacillus	bacillus	NOUN
ajst-25583	75	34	subtilis	subtili	NOUN
ajst-25583	75	35	for	for	ADP
ajst-25583	75	36	104	104	NUM
ajst-25583	75	37	microbial	microbial	ADJ
ajst-25583	75	38	production	production	NOUN
ajst-25583	75	39	of	of	ADP
ajst-25583	75	40	acetylglucosamine	acetylglucosamine	NOUN
ajst-25583	75	41	,	,	PUNCT
ajst-25583	75	42	metabolic	metabolic	NOUN
ajst-25583	75	43	engineering	engineering	NOUN
ajst-25583	75	44	,	,	PUNCT
ajst-25583	75	45	vol	vol	NOUN
ajst-25583	75	46	.	.	PROPN
ajst-25583	75	47	19	19	NUM
ajst-25583	75	48	(	(	PUNCT
ajst-25583	75	49	2013	2013	NUM
ajst-25583	75	50	)	)	PUNCT
ajst-25583	75	51	,	,	PUNCT
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ajst-25583	75	55	.	.	PUNCT
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ajst-25583	76	2	12	12	NUM
ajst-25583	76	3	]	]	X
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ajst-25583	76	5	gu	gu	PROPN
ajst-25583	76	6	,	,	PUNCT
ajst-25583	76	7	x.	x.	PROPN
ajst-25583	76	8	q.	q.	PROPN
ajst-25583	76	9	lv	lv	PROPN
ajst-25583	76	10	,	,	PUNCT
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ajst-25583	76	12	f.	f.	PROPN
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ajst-25583	76	14	,	,	PUNCT
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ajst-25583	76	16	h.	h.	PROPN
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ajst-25583	76	18	,	,	PUNCT
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ajst-25583	76	27	a.	a.	PROPN
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ajst-25583	76	39	redox	redox	ADJ
ajst-25583	76	40	metabolism	metabolism	NOUN
ajst-25583	76	41	for	for	ADP
ajst-25583	76	42	high	high	ADJ
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ajst-25583	76	44	production	production	NOUN
ajst-25583	76	45	of	of	ADP
ajst-25583	76	46	acetylglucosamine	acetylglucosamine	NOUN
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ajst-25583	76	48	bacillus	bacillus	NOUN
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ajst-25583	76	58	2019	2019	NUM
ajst-25583	76	59	)	)	PUNCT
ajst-25583	76	60	,	,	PUNCT
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ajst-25583	76	62	-	-	PUNCT
ajst-25583	76	63	69	69	NUM
ajst-25583	76	64	.	.	PUNCT
ajst-25583	77	1	[	[	X
ajst-25583	77	2	13	13	NUM
ajst-25583	77	3	]	]	PUNCT
ajst-25583	77	4	s.	s.	PROPN
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ajst-25583	77	6	,	,	PUNCT
ajst-25583	77	7	y.	y.	PROPN
ajst-25583	77	8	wang	wang	PROPN
ajst-25583	77	9	,	,	PUNCT
ajst-25583	77	10	c.	c.	PROPN
ajst-25583	77	11	b.	b.	PROPN
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ajst-25583	77	18	x.	x.	PROPN
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ajst-25583	77	34	srna	srna	NOUN
ajst-25583	77	35	-	-	PUNCT
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ajst-25583	77	51	(	(	PUNCT
ajst-25583	77	52	2018	2018	NUM
ajst-25583	77	53	)	)	PUNCT
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ajst-25583	77	55	,	,	PUNCT
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ajst-25583	77	57	-	-	SYM
ajst-25583	77	58	2995	2995	NUM
ajst-25583	77	59	.	.	PUNCT
ajst-25583	78	1	[	[	X
ajst-25583	78	2	14	14	NUM
ajst-25583	78	3	]	]	X
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ajst-25583	78	34	engineered	engineer	VERB
ajst-25583	78	35	bacillus	bacillus	NOUN
ajst-25583	78	36	subtilis	subtili	NOUN
ajst-25583	78	37	,	,	PUNCT
ajst-25583	78	38	carbohydrate	carbohydrate	NOUN
ajst-25583	78	39	polymers	polymer	NOUN
ajst-25583	78	40	,	,	PUNCT
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ajst-25583	78	42	.	.	PROPN
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ajst-25583	78	44	(	(	PUNCT
ajst-25583	78	45	2018	2018	NUM
ajst-25583	78	46	)	)	PUNCT
ajst-25583	78	47	,	,	PUNCT
ajst-25583	78	48	p.424	p.424	X
ajst-25583	78	49	-	-	PUNCT
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ajst-25583	78	51	.	.	PUNCT
ajst-25583	79	1	[	[	X
ajst-25583	79	2	15	15	NUM
ajst-25583	79	3	]	]	X
ajst-25583	79	4	s.	s.	PROPN
ajst-25583	79	5	x.	x.	PROPN
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ajst-25583	79	8	x.	x.	PROPN
ajst-25583	79	9	q.	q.	PROPN
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ajst-25583	79	11	,	,	PUNCT
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ajst-25583	79	13	k.	k.	PROPN
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ajst-25583	79	16	j.	j.	PROPN
ajst-25583	79	17	h.	h.	PROPN
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ajst-25583	79	28	liu	liu	PROPN
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ajst-25583	79	34	-	-	PUNCT
ajst-25583	79	35	rap60spo0a	rap60spo0a	NOUN
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ajst-25583	79	40	switch	switch	NOUN
ajst-25583	79	41	for	for	ADP
ajst-25583	79	42	dynamic	dynamic	ADJ
ajst-25583	79	43	finetuning	finetuning	NOUN
ajst-25583	79	44	of	of	ADP
ajst-25583	79	45	menaquinone-7	menaquinone-7	NUM
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ajst-25583	79	47	in	in	ADP
ajst-25583	79	48	bacillus	bacillus	NOUN
ajst-25583	79	49	subtilis	subtili	NOUN
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ajst-25583	79	59	2019	2019	NUM
ajst-25583	79	60	)	)	PUNCT
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ajst-25583	79	62	,	,	PUNCT
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ajst-25583	79	64	-	-	PUNCT
ajst-25583	79	65	1837	1837	NUM
ajst-25583	79	66	.	.	PUNCT
ajst-25583	80	1	[	[	X
ajst-25583	80	2	16	16	NUM
ajst-25583	80	3	]	]	PUNCT
ajst-25583	80	4	s.	s.	PROPN
ajst-25583	80	5	b.	b.	PROPN
ajst-25583	80	6	shi	shi	PROPN
ajst-25583	80	7	,	,	PUNCT
ajst-25583	80	8	t.	t.	PROPN
ajst-25583	80	9	chen	chen	PROPN
ajst-25583	80	10	,	,	PUNCT
ajst-25583	80	11	z.	z.	PROPN
ajst-25583	80	12	g.	g.	PROPN
ajst-25583	80	13	zhang	zhang	PROPN
ajst-25583	80	14	,	,	PUNCT
ajst-25583	80	15	x.	x.	PROPN
ajst-25583	80	16	chen	chen	PROPN
ajst-25583	80	17	and	and	CCONJ
ajst-25583	80	18	x.	x.	PROPN
ajst-25583	80	19	m.	m.	NOUN
ajst-25583	80	20	zhao	zhao	PROPN
ajst-25583	80	21	:	:	PUNCT
ajst-25583	80	22	transcriptome	transcriptome	ADJ
ajst-25583	80	23	analysis	analysis	NOUN
ajst-25583	80	24	guided	guide	VERB
ajst-25583	80	25	metabolic	metabolic	NOUN
ajst-25583	80	26	engineering	engineering	NOUN
ajst-25583	80	27	of	of	ADP
ajst-25583	80	28	bacillus	bacillus	NOUN
ajst-25583	80	29	subtilis	subtili	NOUN
ajst-25583	80	30	for	for	ADP
ajst-25583	80	31	riboflavin	riboflavin	NOUN
ajst-25583	80	32	production	production	NOUN
ajst-25583	80	33	,	,	PUNCT
ajst-25583	80	34	metabolic	metabolic	NOUN
ajst-25583	80	35	engineering	engineering	NOUN
ajst-25583	80	36	,	,	PUNCT
ajst-25583	80	37	vol	vol	NOUN
ajst-25583	80	38	.	.	PROPN
ajst-25583	80	39	11	11	NUM
ajst-25583	80	40	(	(	PUNCT
ajst-25583	80	41	2009	2009	NUM
ajst-25583	80	42	)	)	PUNCT
ajst-25583	80	43	no.4	no.4	PROPN
ajst-25583	80	44	-	-	SYM
ajst-25583	80	45	5	5	NUM
ajst-25583	80	46	,	,	PUNCT
ajst-25583	80	47	p.243	p.243	VERB
ajst-25583	80	48	-	-	PUNCT
ajst-25583	80	49	252	252	NUM
ajst-25583	80	50	.	.	PUNCT
ajst-25583	81	1	[	[	X
ajst-25583	81	2	17	17	NUM
ajst-25583	81	3	]	]	PUNCT
ajst-25583	81	4	t.	t.	PROPN
ajst-25583	81	5	shi	shi	PROPN
ajst-25583	81	6	,	,	PUNCT
ajst-25583	81	7	y.	y.	PROPN
ajst-25583	81	8	c.	c.	PROPN
ajst-25583	81	9	wang	wang	PROPN
ajst-25583	81	10	,	,	PUNCT
ajst-25583	81	11	z.	z.	PROPN
ajst-25583	81	12	w.	w.	PROPN
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ajst-25583	81	14	,	,	PUNCT
ajst-25583	81	15	g.	g.	PROPN
ajst-25583	81	16	l.	l.	PROPN
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ajst-25583	81	18	,	,	PUNCT
ajst-25583	81	19	d.	d.	PROPN
ajst-25583	81	20	y.	y.	PROPN
ajst-25583	81	21	liu	liu	PROPN
ajst-25583	81	22	,	,	PUNCT
ajst-25583	81	23	j.	j.	PROPN
ajst-25583	81	24	fu	fu	PROPN
ajst-25583	81	25	,	,	PUNCT
ajst-25583	81	26	t.	t.	PROPN
ajst-25583	81	27	chen	chen	PROPN
ajst-25583	81	28	and	and	CCONJ
ajst-25583	81	29	x.	x.	PROPN
ajst-25583	81	30	m.	m.	PROPN
ajst-25583	81	31	zhao	zhao	PROPN
ajst-25583	81	32	:	:	PUNCT
ajst-25583	81	33	deregulation	deregulation	NOUN
ajst-25583	81	34	of	of	ADP
ajst-25583	81	35	purine	purine	NOUN
ajst-25583	81	36	pathway	pathway	NOUN
ajst-25583	81	37	in	in	ADP
ajst-25583	81	38	bacillus	bacillus	NOUN
ajst-25583	81	39	subtilis	subtili	NOUN
ajst-25583	81	40	and	and	CCONJ
ajst-25583	81	41	its	its	PRON
ajst-25583	81	42	use	use	NOUN
ajst-25583	81	43	in	in	ADP
ajst-25583	81	44	riboflavin	riboflavin	NOUN
ajst-25583	81	45	biosynthesis	biosynthesis	NOUN
ajst-25583	81	46	,	,	PUNCT
ajst-25583	81	47	microbial	microbial	ADJ
ajst-25583	81	48	cell	cell	NOUN
ajst-25583	81	49	factories	factory	NOUN
ajst-25583	81	50	,	,	PUNCT
ajst-25583	81	51	vol	vol	NOUN
ajst-25583	81	52	.	.	PROPN
ajst-25583	81	53	13	13	NUM
ajst-25583	81	54	(	(	PUNCT
ajst-25583	81	55	2014	2014	NUM
ajst-25583	81	56	)	)	PUNCT
ajst-25583	81	57	,	,	PUNCT
ajst-25583	81	58	p.101	p.101	NOUN
ajst-25583	81	59	-	-	PUNCT
ajst-25583	81	60	116	116	NUM
ajst-25583	81	61	.	.	PUNCT
ajst-25583	82	1	[	[	X
ajst-25583	82	2	18	18	NUM
ajst-25583	82	3	]	]	X
ajst-25583	82	4	abdallah	abdallah	PROPN
ajst-25583	82	5	,	,	PUNCT
ajst-25583	82	6	ii	ii	PROPN
ajst-25583	82	7	,	,	PUNCT
ajst-25583	82	8	h.	h.	PROPN
ajst-25583	82	9	pramastya	pramastya	PROPN
ajst-25583	82	10	,	,	PUNCT
ajst-25583	82	11	r.	r.	PROPN
ajst-25583	82	12	van	van	PROPN
ajst-25583	82	13	merkerk	merkerk	PROPN
ajst-25583	82	14	,	,	PUNCT
ajst-25583	82	15	sukrasno	sukrasno	NOUN
ajst-25583	82	16	and	and	CCONJ
ajst-25583	82	17	w.	w.	PROPN
ajst-25583	82	18	j.	j.	PROPN
ajst-25583	82	19	quax	quax	PROPN
ajst-25583	82	20	:	:	PUNCT
ajst-25583	82	21	metabolic	metabolic	PROPN
ajst-25583	82	22	engineering	engineering	NOUN
ajst-25583	82	23	of	of	ADP
ajst-25583	82	24	bacillus	bacillus	NOUN
ajst-25583	82	25	subtilis	subtili	NOUN
ajst-25583	82	26	toward	toward	ADP
ajst-25583	82	27	taxadiene	taxadiene	NOUN
ajst-25583	82	28	biosynthesis	biosynthesis	NOUN
ajst-25583	82	29	as	as	ADP
ajst-25583	82	30	the	the	DET
ajst-25583	82	31	first	first	ADJ
ajst-25583	82	32	committed	committed	ADJ
ajst-25583	82	33	step	step	NOUN
ajst-25583	82	34	for	for	ADP
ajst-25583	82	35	taxol	taxol	NOUN
ajst-25583	82	36	production	production	NOUN
ajst-25583	82	37	,	,	PUNCT
ajst-25583	82	38	frontiers	frontier	NOUN
ajst-25583	82	39	in	in	ADP
ajst-25583	82	40	microbiology	microbiology	NOUN
ajst-25583	82	41	,	,	PUNCT
ajst-25583	82	42	vol	vol	NOUN
ajst-25583	82	43	.	.	PROPN
ajst-25583	82	44	10	10	NUM
ajst-25583	82	45	(	(	PUNCT
ajst-25583	82	46	2019	2019	NUM
ajst-25583	82	47	)	)	PUNCT
ajst-25583	82	48	,	,	PUNCT
ajst-25583	82	49	p.218228	p.218228	NOUN
ajst-25583	82	50	.	.	PUNCT
ajst-25583	83	1	[	[	X
ajst-25583	83	2	19	19	NUM
ajst-25583	83	3	]	]	X
ajst-25583	83	4	y.	y.	PROPN
ajst-25583	83	5	gu	gu	PROPN
ajst-25583	83	6	,	,	PUNCT
ajst-25583	83	7	x.	x.	PROPN
ajst-25583	83	8	h.	h.	PROPN
ajst-25583	83	9	xu	xu	PROPN
ajst-25583	83	10	,	,	PUNCT
ajst-25583	83	11	y.	y.	PROPN
ajst-25583	83	12	k.	k.	PROPN
ajst-25583	83	13	wu	wu	PROPN
ajst-25583	83	14	,	,	PUNCT
ajst-25583	83	15	t.	t.	PROPN
ajst-25583	83	16	f.	f.	PROPN
ajst-25583	83	17	niu	niu	PROPN
ajst-25583	83	18	,	,	PUNCT
ajst-25583	83	19	y.	y.	PROPN
ajst-25583	83	20	f.	f.	PROPN
ajst-25583	83	21	liu	liu	PROPN
ajst-25583	83	22	,	,	PUNCT
ajst-25583	83	23	j.	j.	PROPN
ajst-25583	83	24	h.	h.	PROPN
ajst-25583	83	25	li	li	PROPN
ajst-25583	83	26	,	,	PUNCT
ajst-25583	83	27	g.	g.	PROPN
ajst-25583	83	28	c.	c.	PROPN
ajst-25583	83	29	du	du	PROPN
ajst-25583	83	30	and	and	CCONJ
ajst-25583	83	31	l.	l.	PROPN
ajst-25583	83	32	liu	liu	PROPN
ajst-25583	83	33	:	:	PUNCT
ajst-25583	83	34	advances	advance	NOUN
ajst-25583	83	35	and	and	CCONJ
ajst-25583	83	36	prospects	prospect	NOUN
ajst-25583	83	37	of	of	ADP
ajst-25583	83	38	bacillus	bacillus	NOUN
ajst-25583	83	39	subtilis	subtili	NOUN
ajst-25583	83	40	cellular	cellular	ADJ
ajst-25583	83	41	factories	factory	NOUN
ajst-25583	83	42	:	:	PUNCT
ajst-25583	83	43	from	from	ADP
ajst-25583	83	44	rational	rational	ADJ
ajst-25583	83	45	design	design	NOUN
ajst-25583	83	46	to	to	ADP
ajst-25583	83	47	industrial	industrial	ADJ
ajst-25583	83	48	applications	application	NOUN
ajst-25583	83	49	,	,	PUNCT
ajst-25583	83	50	metabolic	metabolic	NOUN
ajst-25583	83	51	engineering	engineering	NOUN
ajst-25583	83	52	,	,	PUNCT
ajst-25583	83	53	vol	vol	NOUN
ajst-25583	83	54	.	.	PROPN
ajst-25583	83	55	50	50	NUM
ajst-25583	83	56	(	(	PUNCT
ajst-25583	83	57	2018	2018	NUM
ajst-25583	83	58	)	)	PUNCT
ajst-25583	83	59	no.4	no.4	PROPN
ajst-25583	83	60	,	,	PUNCT
ajst-25583	83	61	p.109	p.109	NOUN
ajst-25583	83	62	-	-	PUNCT
ajst-25583	83	63	121	121	NUM
ajst-25583	83	64	.	.	PUNCT
ajst-25583	84	1	[	[	X
ajst-25583	84	2	20	20	NUM
ajst-25583	84	3	]	]	X
ajst-25583	84	4	y.	y.	PROPN
ajst-25583	84	5	f.	f.	PROPN
ajst-25583	84	6	liu	liu	PROPN
ajst-25583	84	7	,	,	PUNCT
ajst-25583	84	8	l.	l.	PROPN
ajst-25583	84	9	liu	liu	PROPN
ajst-25583	84	10	,	,	PUNCT
ajst-25583	84	11	j.	j.	PROPN
ajst-25583	84	12	h.	h.	PROPN
ajst-25583	84	13	li	li	PROPN
ajst-25583	84	14	,	,	PUNCT
ajst-25583	84	15	g.	g.	PROPN
ajst-25583	84	16	c.	c.	PROPN
ajst-25583	84	17	du	du	PROPN
ajst-25583	84	18	and	and	CCONJ
ajst-25583	84	19	j.	j.	PROPN
ajst-25583	84	20	che	che	PROPN
ajst-25583	84	21	:	:	PUNCT
ajst-25583	84	22	synthetic	synthetic	ADJ
ajst-25583	84	23	biology	biology	NOUN
ajst-25583	84	24	toolbox	toolbox	NOUN
ajst-25583	84	25	and	and	CCONJ
ajst-25583	84	26	chassis	chassis	VERB
ajst-25583	84	27	development	development	NOUN
ajst-25583	84	28	in	in	ADP
ajst-25583	84	29	bacillus	bacillus	NOUN
ajst-25583	84	30	subtilis	subtili	NOUN
ajst-25583	84	31	,	,	PUNCT
ajst-25583	84	32	trends	trend	NOUN
ajst-25583	84	33	in	in	ADP
ajst-25583	84	34	biotechnology	biotechnology	NOUN
ajst-25583	84	35	,	,	PUNCT
ajst-25583	84	36	vol	vol	NOUN
ajst-25583	84	37	.	.	PROPN
ajst-25583	84	38	37	37	NUM
ajst-25583	84	39	(	(	PUNCT
ajst-25583	84	40	2019	2019	NUM
ajst-25583	84	41	)	)	PUNCT
ajst-25583	84	42	no.5	no.5	PROPN
ajst-25583	84	43	,	,	PUNCT
ajst-25583	84	44	p.548	p.548	X
ajst-25583	84	45	-	-	NOUN
ajst-25583	84	46	562	562	NUM
ajst-25583	84	47	.	.	PUNCT
ajst-25583	85	1	[	[	X
ajst-25583	85	2	21	21	NUM
ajst-25583	85	3	]	]	PUNCT
ajst-25583	85	4	m.	m.	NOUN
ajst-25583	85	5	j.	j.	PROPN
ajst-25583	85	6	xiang	xiang	PROPN
ajst-25583	85	7	,	,	PUNCT
ajst-25583	85	8	q.	q.	PROPN
ajst-25583	85	9	kang	kang	PROPN
ajst-25583	85	10	and	and	CCONJ
ajst-25583	85	11	d.	d.	PROPN
ajst-25583	85	12	w.	w.	PROPN
ajst-25583	85	13	zhang	zhang	PROPN
ajst-25583	85	14	:	:	PUNCT
ajst-25583	85	15	advances	advance	NOUN
ajst-25583	85	16	on	on	ADP
ajst-25583	85	17	systems	system	NOUN
ajst-25583	85	18	metabolic	metabolic	NOUN
ajst-25583	85	19	engineering	engineering	NOUN
ajst-25583	85	20	of	of	ADP
ajst-25583	85	21	bacillus	bacillus	NOUN
ajst-25583	85	22	subtilis	subtili	NOUN
ajst-25583	85	23	as	as	ADP
ajst-25583	85	24	a	a	DET
ajst-25583	85	25	chassis	chassis	NOUN
ajst-25583	85	26	cell	cell	NOUN
ajst-25583	85	27	,	,	PUNCT
ajst-25583	85	28	synthetic	synthetic	NOUN
ajst-25583	85	29	and	and	CCONJ
ajst-25583	85	30	systems	system	NOUN
ajst-25583	85	31	biotechnology	biotechnology	NOUN
ajst-25583	85	32	,	,	PUNCT
ajst-25583	85	33	vol	vol	NOUN
ajst-25583	85	34	.	.	PROPN
ajst-25583	85	35	5	5	NUM
ajst-25583	85	36	(	(	PUNCT
ajst-25583	85	37	2020	2020	NUM
ajst-25583	85	38	)	)	PUNCT
ajst-25583	85	39	no.4	no.4	PROPN
ajst-25583	85	40	,	,	PUNCT
ajst-25583	85	41	p.245	p.245	NOUN
ajst-25583	85	42	-	-	SYM
ajst-25583	85	43	251	251	NUM
ajst-25583	85	44	.	.	PUNCT
ajst-25583	86	1	[	[	X
ajst-25583	86	2	22	22	NUM
ajst-25583	86	3	]	]	PUNCT
ajst-25583	86	4	s.	s.	PROPN
ajst-25583	86	5	yang	yang	PROPN
ajst-25583	86	6	,	,	PUNCT
ajst-25583	86	7	z.	z.	PROPN
ajst-25583	86	8	kang	kang	PROPN
ajst-25583	86	9	,	,	PUNCT
ajst-25583	86	10	w.	w.	PROPN
ajst-25583	86	11	l.	l.	PROPN
ajst-25583	86	12	cao	cao	PROPN
ajst-25583	86	13	,	,	PUNCT
ajst-25583	86	14	g.	g.	PROPN
ajst-25583	86	15	c.	c.	PROPN
ajst-25583	86	16	du	du	PROPN
ajst-25583	86	17	and	and	CCONJ
ajst-25583	86	18	j.	j.	PROPN
ajst-25583	86	19	chen	chen	PROPN
ajst-25583	86	20	:	:	PUNCT
ajst-25583	86	21	construction	construction	NOUN
ajst-25583	86	22	of	of	ADP
ajst-25583	86	23	a	a	DET
ajst-25583	86	24	novel	novel	NOUN
ajst-25583	86	25	,	,	PUNCT
ajst-25583	86	26	stable	stable	ADJ
ajst-25583	86	27	,	,	PUNCT
ajst-25583	86	28	food	food	NOUN
ajst-25583	86	29	-	-	PUNCT
ajst-25583	86	30	grade	grade	NOUN
ajst-25583	86	31	expression	expression	NOUN
ajst-25583	86	32	system	system	NOUN
ajst-25583	86	33	by	by	ADP
ajst-25583	86	34	engineering	engineer	VERB
ajst-25583	86	35	the	the	DET
ajst-25583	86	36	endogenous	endogenous	ADJ
ajst-25583	86	37	toxin	toxin	NOUN
ajst-25583	86	38	-	-	PUNCT
ajst-25583	86	39	antitoxin	antitoxin	NOUN
ajst-25583	86	40	system	system	NOUN
ajst-25583	86	41	in	in	ADP
ajst-25583	86	42	bacillus	bacillus	NOUN
ajst-25583	86	43	subtilis	subtili	NOUN
ajst-25583	86	44	,	,	PUNCT
ajst-25583	86	45	journal	journal	NOUN
ajst-25583	86	46	of	of	ADP
ajst-25583	86	47	biotechnology	biotechnology	NOUN
ajst-25583	86	48	,	,	PUNCT
ajst-25583	86	49	vol	vol	NOUN
ajst-25583	86	50	.	.	PROPN
ajst-25583	86	51	219	219	NUM
ajst-25583	86	52	(	(	PUNCT
ajst-25583	86	53	2022	2022	NUM
ajst-25583	86	54	)	)	PUNCT
ajst-25583	86	55	,	,	PUNCT
ajst-25583	86	56	p.40	p.40	NOUN
ajst-25583	86	57	-	-	PROPN
ajst-25583	86	58	47	47	NUM
ajst-25583	86	59	.	.	PUNCT
ajst-25583	87	1	[	[	X
ajst-25583	87	2	23	23	NUM
ajst-25583	87	3	]	]	PUNCT
ajst-25583	87	4	l.	l.	PROPN
ajst-25583	87	5	a.	a.	PROPN
ajst-25583	87	6	flórez	flórez	PROPN
ajst-25583	87	7	,	,	PUNCT
ajst-25583	87	8	s.	s.	PROPN
ajst-25583	87	9	f.	f.	PROPN
ajst-25583	87	10	roppel	roppel	PROPN
ajst-25583	87	11	,	,	PUNCT
ajst-25583	87	12	a.	a.	NOUN
ajst-25583	87	13	g.	g.	PROPN
ajst-25583	87	14	schmeisky	schmeisky	PROPN
ajst-25583	87	15	,	,	PUNCT
ajst-25583	87	16	c.	c.	PROPN
ajst-25583	87	17	r.	r.	PROPN
ajst-25583	87	18	lammers	lammers	PROPN
ajst-25583	87	19	and	and	CCONJ
ajst-25583	87	20	j.	j.	PROPN
ajst-25583	87	21	stülke	stülke	PROPN
ajst-25583	87	22	:	:	PUNCT
ajst-25583	87	23	a	a	DET
ajst-25583	87	24	community	community	NOUN
ajst-25583	87	25	-	-	PUNCT
ajst-25583	87	26	curated	curate	VERB
ajst-25583	87	27	consensual	consensual	ADJ
ajst-25583	87	28	annotation	annotation	NOUN
ajst-25583	87	29	that	that	PRON
ajst-25583	87	30	is	be	AUX
ajst-25583	87	31	continuously	continuously	ADV
ajst-25583	87	32	updated	update	VERB
ajst-25583	87	33	:	:	PUNCT
ajst-25583	87	34	the	the	DET
ajst-25583	87	35	bacillus	bacillus	NOUN
ajst-25583	87	36	subtilis	subtili	VERB
ajst-25583	87	37	centred	centre	VERB
ajst-25583	87	38	wiki	wiki	PROPN
ajst-25583	87	39	subti	subti	PROPN
ajst-25583	87	40	wiki	wiki	PROPN
ajst-25583	87	41	,	,	PUNCT
ajst-25583	87	42	database	database	NOUN
ajst-25583	87	43	-	-	PUNCT
ajst-25583	87	44	the	the	DET
ajst-25583	87	45	journal	journal	NOUN
ajst-25583	87	46	of	of	ADP
ajst-25583	87	47	biological	biological	ADJ
ajst-25583	87	48	databases	database	NOUN
ajst-25583	87	49	and	and	CCONJ
ajst-25583	87	50	curation	curation	NOUN
ajst-25583	87	51	,	,	PUNCT
ajst-25583	87	52	vol	vol	NOUN
ajst-25583	87	53	.	.	PROPN
ajst-25583	87	54	2009	2009	NUM
ajst-25583	87	55	(	(	PUNCT
ajst-25583	87	56	2009	2009	NUM
ajst-25583	87	57	)	)	PUNCT
ajst-25583	87	58	,	,	PUNCT
ajst-25583	87	59	p.9	p.9	X
ajst-25583	87	60	.	.	PUNCT
ajst-25583	88	1	[	[	X
ajst-25583	88	2	24	24	NUM
ajst-25583	88	3	]	]	X
ajst-25583	88	4	t.	t.	NOUN
ajst-25583	88	5	ishii	ishii	PROPN
ajst-25583	88	6	,	,	PUNCT
ajst-25583	88	7	k.	k.	PROPN
ajst-25583	88	8	yoshida	yoshida	PROPN
ajst-25583	88	9	,	,	PUNCT
ajst-25583	88	10	g.	g.	PROPN
ajst-25583	88	11	terai	terai	PROPN
ajst-25583	88	12	,	,	PUNCT
ajst-25583	88	13	y.	y.	PROPN
ajst-25583	88	14	fujita	fujita	PROPN
ajst-25583	88	15	and	and	CCONJ
ajst-25583	88	16	k.	k.	PROPN
ajst-25583	88	17	nakai	nakai	PROPN
ajst-25583	88	18	:	:	PUNCT
ajst-25583	88	19	dbtbs	dbtbs	PROPN
ajst-25583	88	20	:	:	PUNCT
ajst-25583	88	21	a	a	DET
ajst-25583	88	22	database	database	NOUN
ajst-25583	88	23	of	of	ADP
ajst-25583	88	24	bacillus	bacillus	NOUN
ajst-25583	88	25	subtilis	subtilis	NOUN
ajst-25583	88	26	promoters	promoter	NOUN
ajst-25583	88	27	and	and	CCONJ
ajst-25583	88	28	transcription	transcription	NOUN
ajst-25583	88	29	factors	factor	NOUN
ajst-25583	88	30	,	,	PUNCT
ajst-25583	88	31	nucleic	nucleic	ADJ
ajst-25583	88	32	acids	acid	NOUN
ajst-25583	88	33	research	research	NOUN
ajst-25583	88	34	,	,	PUNCT
ajst-25583	88	35	vol	vol	NOUN
ajst-25583	88	36	.	.	PROPN
ajst-25583	88	37	29	29	NUM
ajst-25583	88	38	(	(	PUNCT
ajst-25583	88	39	2001	2001	NUM
ajst-25583	88	40	)	)	PUNCT
ajst-25583	88	41	no.1	no.1	NOUN
ajst-25583	88	42	,	,	PUNCT
ajst-25583	88	43	p.278	p.278	NUM
ajst-25583	88	44	-	-	PUNCT
ajst-25583	88	45	80	80	NUM
ajst-25583	88	46	.	.	PUNCT
ajst-25583	89	1	[	[	X
ajst-25583	89	2	25	25	NUM
ajst-25583	89	3	]	]	X
ajst-25583	89	4	p.	p.	PROPN
ajst-25583	89	5	d.	d.	PROPN
ajst-25583	89	6	karp	karp	PROPN
ajst-25583	89	7	,	,	PUNCT
ajst-25583	89	8	c.	c.	PROPN
ajst-25583	89	9	a.	a.	PROPN
ajst-25583	89	10	ouzounis	ouzounis	PROPN
ajst-25583	89	11	,	,	PUNCT
ajst-25583	89	12	c.	c.	PROPN
ajst-25583	89	13	moore	moore	PROPN
ajst-25583	89	14	-	-	PUNCT
ajst-25583	89	15	kochlacs	kochlacs	PROPN
ajst-25583	89	16	,	,	PUNCT
ajst-25583	89	17	l.	l.	PROPN
ajst-25583	89	18	goldovsky	goldovsky	PROPN
ajst-25583	89	19	,	,	PUNCT
ajst-25583	89	20	p.	p.	PROPN
ajst-25583	89	21	kaipa	kaipa	PROPN
ajst-25583	89	22	,	,	PUNCT
ajst-25583	89	23	d.	d.	PROPN
ajst-25583	89	24	ahren	ahren	PROPN
ajst-25583	89	25	,	,	PUNCT
ajst-25583	89	26	s.	s.	PROPN
ajst-25583	89	27	tsoka	tsoka	PROPN
ajst-25583	89	28	,	,	PUNCT
ajst-25583	89	29	n.	n.	NOUN
ajst-25583	89	30	darzentas	darzenta	NOUN
ajst-25583	89	31	,	,	PUNCT
ajst-25583	89	32	v.	v.	ADP
ajst-25583	89	33	kunin	kunin	PROPN
ajst-25583	89	34	and	and	CCONJ
ajst-25583	89	35	n.	n.	PROPN
ajst-25583	89	36	lopez	lopez	PROPN
ajst-25583	89	37	-	-	PUNCT
ajst-25583	89	38	bigas	bigas	NOUN
ajst-25583	89	39	:	:	PUNCT
ajst-25583	89	40	expansion	expansion	NOUN
ajst-25583	89	41	of	of	ADP
ajst-25583	89	42	the	the	DET
ajst-25583	89	43	biocyc	biocyc	NOUN
ajst-25583	89	44	collection	collection	NOUN
ajst-25583	89	45	of	of	ADP
ajst-25583	89	46	pathway	pathway	NOUN
ajst-25583	89	47	/	/	SYM
ajst-25583	89	48	genome	genome	NOUN
ajst-25583	89	49	databases	database	NOUN
ajst-25583	89	50	to	to	ADP
ajst-25583	89	51	160	160	NUM
ajst-25583	89	52	genomes	genome	NOUN
ajst-25583	89	53	,	,	PUNCT
ajst-25583	89	54	nucleic	nucleic	ADJ
ajst-25583	89	55	acids	acid	NOUN
ajst-25583	89	56	research	research	NOUN
ajst-25583	89	57	,	,	PUNCT
ajst-25583	89	58	vol	vol	NOUN
ajst-25583	89	59	.	.	PROPN
ajst-25583	90	1	33	33	NUM
ajst-25583	90	2	(	(	PUNCT
ajst-25583	90	3	2005	2005	NUM
ajst-25583	90	4	)	)	PUNCT
ajst-25583	90	5	no.19	no.19	PROPN
ajst-25583	90	6	,	,	PUNCT
ajst-25583	90	7	p.6083	p.6083	NOUN
ajst-25583	90	8	-	-	PUNCT
ajst-25583	90	9	9	9	NUM
ajst-25583	90	10	.	.	PUNCT
ajst-25583	91	1	[	[	X
ajst-25583	91	2	26	26	NUM
ajst-25583	91	3	]	]	PUNCT
ajst-25583	91	4	m.	m.	NOUN
ajst-25583	91	5	x.	x.	PROPN
ajst-25583	91	6	zhang	zhang	PROPN
ajst-25583	91	7	,	,	PUNCT
ajst-25583	91	8	x.	x.	PROPN
ajst-25583	91	9	c.	c.	PROPN
ajst-25583	91	10	zhao	zhao	PROPN
ajst-25583	91	11	,	,	PUNCT
ajst-25583	91	12	x.	x.	PROPN
ajst-25583	91	13	chen	chen	PROPN
ajst-25583	91	14	,	,	PUNCT
ajst-25583	91	15	m.	m.	PROPN
ajst-25583	91	16	y.	y.	PROPN
ajst-25583	91	17	li	li	PROPN
ajst-25583	91	18	and	and	CCONJ
ajst-25583	91	19	x.	x.	PROPN
ajst-25583	91	20	d.	d.	PROPN
ajst-25583	91	21	wang	wang	PROPN
ajst-25583	91	22	:	:	PUNCT
ajst-25583	91	23	enhancement	enhancement	NOUN
ajst-25583	91	24	of	of	ADP
ajst-25583	91	25	riboflavin	riboflavin	NOUN
ajst-25583	91	26	production	production	NOUN
ajst-25583	91	27	in	in	ADP
ajst-25583	91	28	bacillus	bacillus	NOUN
ajst-25583	91	29	subtilis	subtili	NOUN
ajst-25583	91	30	via	via	ADP
ajst-25583	91	31	in	in	ADP
ajst-25583	91	32	vitro	vitro	X
ajst-25583	91	33	and	and	CCONJ
ajst-25583	91	34	in	in	ADP
ajst-25583	91	35	vivo	vivo	ADJ
ajst-25583	91	36	metabolic	metabolic	NOUN
ajst-25583	91	37	engineering	engineering	NOUN
ajst-25583	91	38	of	of	ADP
ajst-25583	91	39	pentose	pentose	NOUN
ajst-25583	91	40	phosphate	phosphate	ADJ
ajst-25583	91	41	pathway	pathway	NOUN
ajst-25583	91	42	,	,	PUNCT
ajst-25583	91	43	biotechnology	biotechnology	NOUN
ajst-25583	91	44	letters	letter	NOUN
ajst-25583	91	45	,	,	PUNCT
ajst-25583	91	46	vol	vol	NOUN
ajst-25583	91	47	.	.	PROPN
ajst-25583	91	48	43	43	NUM
ajst-25583	91	49	(	(	PUNCT
ajst-25583	91	50	2021	2021	NUM
ajst-25583	91	51	)	)	PUNCT
ajst-25583	91	52	no.12	no.12	NOUN
ajst-25583	91	53	,	,	PUNCT
ajst-25583	91	54	p.2209	p.2209	NOUN
ajst-25583	91	55	-	-	PUNCT
ajst-25583	91	56	2216	2216	NUM
ajst-25583	91	57	.	.	PUNCT
ajst-25583	92	1	[	[	X
ajst-25583	92	2	27	27	NUM
ajst-25583	92	3	]	]	X
ajst-25583	92	4	p.	p.	NOUN
ajst-25583	92	5	jin	jin	PROPN
ajst-25583	92	6	,	,	PUNCT
ajst-25583	92	7	z.	z.	PROPN
ajst-25583	92	8	kang	kang	PROPN
ajst-25583	92	9	,	,	PUNCT
ajst-25583	92	10	p.	p.	PROPN
ajst-25583	92	11	h.	h.	PROPN
ajst-25583	92	12	yuan	yuan	PROPN
ajst-25583	92	13	,	,	PUNCT
ajst-25583	92	14	g.	g.	PROPN
ajst-25583	92	15	c.	c.	PROPN
ajst-25583	92	16	du	du	PROPN
ajst-25583	92	17	and	and	CCONJ
ajst-25583	92	18	j.	j.	PROPN
ajst-25583	92	19	chen	chen	PROPN
ajst-25583	92	20	:	:	PUNCT
ajst-25583	92	21	production	production	NOUN
ajst-25583	92	22	of	of	ADP
ajst-25583	92	23	specific	specific	ADJ
ajst-25583	92	24	-	-	ADJ
ajst-25583	92	25	molecular	molecular	ADJ
ajst-25583	92	26	-	-	PUNCT
ajst-25583	92	27	weight	weight	NOUN
ajst-25583	92	28	hyaluronan	hyaluronan	NOUN
ajst-25583	92	29	by	by	ADP
ajst-25583	92	30	metabolically	metabolically	ADV
ajst-25583	92	31	engineered	engineer	VERB
ajst-25583	92	32	bacillus	bacillus	NOUN
ajst-25583	92	33	subtilis	subtili	NOUN
ajst-25583	92	34	168	168	NUM
ajst-25583	92	35	,	,	PUNCT
ajst-25583	92	36	metabolic	metabolic	NOUN
ajst-25583	92	37	engineering	engineering	NOUN
ajst-25583	92	38	,	,	PUNCT
ajst-25583	92	39	vol	vol	NOUN
ajst-25583	92	40	.	.	PROPN
ajst-25583	92	41	35	35	NUM
ajst-25583	92	42	(	(	PUNCT
ajst-25583	92	43	2016	2016	NUM
ajst-25583	92	44	)	)	PUNCT
ajst-25583	92	45	,	,	PUNCT
ajst-25583	92	46	p.21	p.21	NOUN
ajst-25583	92	47	-	-	PUNCT
ajst-25583	92	48	30	30	NUM
ajst-25583	92	49	[	[	SYM
ajst-25583	92	50	28	28	NUM
ajst-25583	92	51	]	]	X
ajst-25583	92	52	y.	y.	PROPN
ajst-25583	92	53	wang	wang	PROPN
ajst-25583	92	54	,	,	PUNCT
ajst-25583	92	55	j.	j.	PROPN
ajst-25583	92	56	weng	weng	PROPN
ajst-25583	92	57	,	,	PUNCT
ajst-25583	92	58	r.	r.	PROPN
ajst-25583	92	59	waseem	waseem	PROPN
ajst-25583	92	60	,	,	PUNCT
ajst-25583	92	61	x.	x.	PROPN
ajst-25583	92	62	h.	h.	PROPN
ajst-25583	92	63	yin	yin	PROPN
ajst-25583	92	64	,	,	PUNCT
ajst-25583	92	65	r.	r.	PROPN
ajst-25583	92	66	f.	f.	PROPN
ajst-25583	92	67	zhang	zhang	PROPN
ajst-25583	92	68	and	and	CCONJ
ajst-25583	92	69	q.	q.	PROPN
ajst-25583	92	70	r.	r.	PROPN
ajst-25583	92	71	shen	shen	PROPN
ajst-25583	92	72	:	:	PUNCT
ajst-25583	92	73	bacillus	bacillus	NOUN
ajst-25583	92	74	subtilis	subtili	NOUN
ajst-25583	92	75	genome	genome	NOUN
ajst-25583	92	76	editing	editing	NOUN
ajst-25583	92	77	using	use	VERB
ajst-25583	92	78	ssdna	ssdna	ADV
ajst-25583	92	79	with	with	ADP
ajst-25583	92	80	short	short	ADJ
ajst-25583	92	81	homology	homology	NOUN
ajst-25583	92	82	regions	region	NOUN
ajst-25583	92	83	,	,	PUNCT
ajst-25583	92	84	nucleic	nucleic	ADJ
ajst-25583	92	85	acids	acid	NOUN
ajst-25583	92	86	research	research	NOUN
ajst-25583	92	87	2012	2012	NUM
ajst-25583	92	88	,	,	PUNCT
ajst-25583	92	89	40	40	NUM
ajst-25583	92	90	:	:	SYM
ajst-25583	92	91	9	9	NUM
ajst-25583	92	92	[	[	SYM
ajst-25583	92	93	29	29	NUM
ajst-25583	92	94	]	]	PUNCT
ajst-25583	92	95	x.	x.	PROPN
ajst-25583	92	96	yan	yan	PROPN
ajst-25583	92	97	,	,	PUNCT
ajst-25583	92	98	h.	h.	PROPN
ajst-25583	92	99	j.	j.	PROPN
ajst-25583	92	100	yu	yu	PROPN
ajst-25583	92	101	,	,	PUNCT
ajst-25583	92	102	q.	q.	PROPN
ajst-25583	92	103	hong	hong	PROPN
ajst-25583	92	104	and	and	CCONJ
ajst-25583	92	105	s.	s.	PROPN
ajst-25583	92	106	p.	p.	PROPN
ajst-25583	92	107	li	li	PROPN
ajst-25583	92	108	:	:	PUNCT
ajst-25583	92	109	crelox	crelox	NOUN
ajst-25583	92	110	system	system	NOUN
ajst-25583	92	111	and	and	CCONJ
ajst-25583	92	112	pcr	pcr	NOUN
ajst-25583	92	113	-	-	PUNCT
ajst-25583	92	114	based	base	VERB
ajst-25583	92	115	genome	genome	NOUN
ajst-25583	92	116	engineering	engineering	NOUN
ajst-25583	92	117	in	in	ADP
ajst-25583	92	118	bacillus	bacillus	NOUN
ajst-25583	92	119	subtilis	subtili	NOUN
ajst-25583	92	120	,	,	PUNCT
ajst-25583	92	121	applied	apply	VERB
ajst-25583	92	122	and	and	CCONJ
ajst-25583	92	123	environmental	environmental	ADJ
ajst-25583	92	124	microbiology	microbiology	NOUN
ajst-25583	92	125	,	,	PUNCT
ajst-25583	92	126	vol	vol	NOUN
ajst-25583	92	127	.	.	PROPN
ajst-25583	92	128	74	74	NUM
ajst-25583	92	129	(	(	PUNCT
ajst-25583	92	130	2008	2008	NUM
ajst-25583	92	131	)	)	PUNCT
ajst-25583	92	132	no.17	no.17	NOUN
ajst-25583	92	133	,	,	PUNCT
ajst-25583	92	134	p.5556	p.5556	NOUN
ajst-25583	92	135	-	-	PUNCT
ajst-25583	92	136	62	62	NUM
ajst-25583	92	137	.	.	PUNCT
ajst-25583	93	1	[	[	X
ajst-25583	93	2	30	30	NUM
ajst-25583	93	3	]	]	PUNCT
ajst-25583	93	4	p.	p.	PROPN
ajst-25583	93	5	f.	f.	PROPN
ajst-25583	93	6	popp	popp	PROPN
ajst-25583	93	7	,	,	PUNCT
ajst-25583	93	8	m.	m.	NOUN
ajst-25583	93	9	dotzler	dotzler	NOUN
ajst-25583	93	10	,	,	PUNCT
ajst-25583	93	11	j.	j.	PROPN
ajst-25583	93	12	radeck	radeck	PROPN
ajst-25583	93	13	,	,	PUNCT
ajst-25583	93	14	j.	j.	PROPN
ajst-25583	93	15	bartels	bartels	PROPN
ajst-25583	93	16	and	and	CCONJ
ajst-25583	93	17	t.	t.	PROPN
ajst-25583	93	18	mascher	mascher	NOUN
ajst-25583	93	19	:	:	PUNCT
ajst-25583	93	20	the	the	DET
ajst-25583	93	21	bacillus	bacillus	NOUN
ajst-25583	93	22	biobrick	biobrick	NOUN
ajst-25583	93	23	box	box	PROPN
ajst-25583	93	24	2.0	2.0	NUM
ajst-25583	93	25	:	:	PUNCT
ajst-25583	93	26	expanding	expand	VERB
ajst-25583	93	27	the	the	DET
ajst-25583	93	28	genetic	genetic	ADJ
ajst-25583	93	29	toolbox	toolbox	NOUN
ajst-25583	93	30	for	for	ADP
ajst-25583	93	31	the	the	DET
ajst-25583	93	32	standardized	standardized	ADJ
ajst-25583	93	33	work	work	NOUN
ajst-25583	93	34	with	with	ADP
ajst-25583	93	35	bacillus	bacillus	NOUN
ajst-25583	93	36	subtilis	subtili	NOUN
ajst-25583	93	37	,	,	PUNCT
ajst-25583	93	38	scientific	scientific	ADJ
ajst-25583	93	39	reports	report	NOUN
ajst-25583	93	40	,	,	PUNCT
ajst-25583	93	41	vol	vol	NOUN
ajst-25583	93	42	.	.	PROPN
ajst-25583	93	43	7	7	NUM
ajst-25583	93	44	(	(	PUNCT
ajst-25583	93	45	2017	2017	NUM
ajst-25583	93	46	)	)	PUNCT
ajst-25583	93	47	no.15058	no.15058	PROPN
ajst-25583	93	48	,	,	PUNCT
ajst-25583	93	49	p.16	p.16	PRON
ajst-25583	93	50	[	[	X
ajst-25583	93	51	31	31	NUM
ajst-25583	93	52	]	]	PUNCT
ajst-25583	93	53	a.	a.	PROPN
ajst-25583	93	54	w.	w.	PROPN
ajst-25583	93	55	westbrook	westbrook	PROPN
ajst-25583	93	56	,	,	PUNCT
ajst-25583	93	57	m.	m.	NOUN
ajst-25583	93	58	moo	moo	NOUN
ajst-25583	93	59	-	-	PUNCT
ajst-25583	93	60	young	young	ADJ
ajst-25583	93	61	and	and	CCONJ
ajst-25583	93	62	c.	c.	PROPN
ajst-25583	93	63	p.	p.	PROPN
ajst-25583	93	64	chou	chou	NOUN
ajst-25583	93	65	:	:	PUNCT
ajst-25583	93	66	development	development	NOUN
ajst-25583	93	67	of	of	ADP
ajst-25583	93	68	a	a	DET
ajst-25583	93	69	crispr	crispr	ADJ
ajst-25583	93	70	-	-	PUNCT
ajst-25583	93	71	cas9	cas9	NOUN
ajst-25583	93	72	tool	tool	NOUN
ajst-25583	93	73	kit	kit	NOUN
ajst-25583	93	74	for	for	ADP
ajst-25583	93	75	comprehensive	comprehensive	ADJ
ajst-25583	93	76	engineering	engineering	NOUN
ajst-25583	93	77	of	of	ADP
ajst-25583	93	78	bacillus	bacillus	NOUN
ajst-25583	93	79	subtilis	subtili	NOUN
ajst-25583	93	80	,	,	PUNCT
ajst-25583	93	81	applied	apply	VERB
ajst-25583	93	82	and	and	CCONJ
ajst-25583	93	83	environmental	environmental	ADJ
ajst-25583	93	84	microbiology	microbiology	NOUN
ajst-25583	93	85	,	,	PUNCT
ajst-25583	93	86	vol	vol	NOUN
ajst-25583	93	87	.	.	PUNCT
ajst-25583	94	1	82	82	NUM
ajst-25583	94	2	(	(	PUNCT
ajst-25583	94	3	2016	2016	NUM
ajst-25583	94	4	)	)	PUNCT
ajst-25583	94	5	no.16	no.16	PROPN
ajst-25583	94	6	,	,	PUNCT
ajst-25583	94	7	p.4876	p.4876	NOUN
ajst-25583	94	8	-	-	NOUN
ajst-25583	94	9	4895	4895	NUM
ajst-25583	94	10	.	.	PUNCT
ajst-25583	95	1	[	[	X
ajst-25583	95	2	32	32	NUM
ajst-25583	95	3	]	]	PUNCT
ajst-25583	95	4	k.	k.	PROPN
ajst-25583	95	5	zhang	zhang	PROPN
ajst-25583	95	6	,	,	PUNCT
ajst-25583	95	7	x.	x.	PROPN
ajst-25583	95	8	g.	g.	PROPN
ajst-25583	95	9	duan	duan	PROPN
ajst-25583	95	10	and	and	CCONJ
ajst-25583	95	11	j.	j.	PROPN
ajst-25583	95	12	wu	wu	PROPN
ajst-25583	95	13	:	:	PUNCT
ajst-25583	95	14	multigene	multigene	VERB
ajst-25583	95	15	disruption	disruption	NOUN
ajst-25583	95	16	in	in	ADP
ajst-25583	95	17	undomesticated	undomesticated	ADJ
ajst-25583	95	18	bacillus	bacillus	NOUN
ajst-25583	95	19	subtilis	subtili	NOUN
ajst-25583	95	20	atcc	atcc	PROPN
ajst-25583	95	21	6051a	6051a	NUM
ajst-25583	95	22	using	use	VERB
ajst-25583	95	23	the	the	DET
ajst-25583	95	24	crispr	crispr	ADJ
ajst-25583	95	25	/	/	SYM
ajst-25583	95	26	cas9	cas9	NOUN
ajst-25583	95	27	system	system	NOUN
ajst-25583	95	28	,	,	PUNCT
ajst-25583	95	29	scientific	scientific	ADJ
ajst-25583	95	30	reports	report	NOUN
ajst-25583	95	31	,	,	PUNCT
ajst-25583	95	32	vol	vol	NOUN
ajst-25583	95	33	.	.	PROPN
ajst-25583	95	34	6	6	NUM
ajst-25583	95	35	(	(	PUNCT
ajst-25583	95	36	2016	2016	NUM
ajst-25583	95	37	)	)	PUNCT
ajst-25583	95	38	,	,	PUNCT
ajst-25583	95	39	p.27943	p.27943	X
ajst-25583	95	40	-	-	NOUN
ajst-25583	95	41	27953	27953	NUM
ajst-25583	95	42	.	.	PUNCT
ajst-25583	96	1	[	[	X
ajst-25583	96	2	33	33	NUM
ajst-25583	96	3	]	]	X
ajst-25583	96	4	y.	y.	NOUN
ajst-25583	97	1	so	so	ADV
ajst-25583	97	2	,	,	PUNCT
ajst-25583	97	3	s.	s.	PROPN
ajst-25583	97	4	y.	y.	PROPN
ajst-25583	97	5	park	park	PROPN
ajst-25583	97	6	,	,	PUNCT
ajst-25583	97	7	e.	e.	PROPN
ajst-25583	97	8	h.	h.	PROPN
ajst-25583	97	9	park	park	PROPN
ajst-25583	97	10	,	,	PUNCT
ajst-25583	97	11	s.	s.	PROPN
ajst-25583	97	12	h.	h.	PROPN
ajst-25583	97	13	park	park	PROPN
ajst-25583	97	14	,	,	PUNCT
ajst-25583	97	15	e.	e.	PROPN
ajst-25583	97	16	j.	j.	PROPN
ajst-25583	97	17	kim	kim	PROPN
ajst-25583	97	18	,	,	PUNCT
ajst-25583	97	19	j.	j.	PROPN
ajst-25583	97	20	g.	g.	PROPN
ajst-25583	97	21	pan	pan	PROPN
ajst-25583	97	22	and	and	CCONJ
ajst-25583	97	23	s.	s.	PROPN
ajst-25583	97	24	k.	k.	PROPN
ajst-25583	97	25	choi	choi	PROPN
ajst-25583	97	26	:	:	PUNCT
ajst-25583	97	27	a	a	DET
ajst-25583	97	28	highly	highly	ADV
ajst-25583	97	29	efficient	efficient	ADJ
ajst-25583	97	30	crispr	crispr	ADJ
ajst-25583	97	31	-	-	PUNCT
ajst-25583	97	32	cas9	cas9	NOUN
ajst-25583	97	33	-	-	PUNCT
ajst-25583	97	34	mediated	mediate	VERB
ajst-25583	97	35	large	large	ADJ
ajst-25583	97	36	genomic	genomic	NOUN
ajst-25583	97	37	deletion	deletion	NOUN
ajst-25583	97	38	in	in	ADP
ajst-25583	97	39	bacillus	bacillus	NOUN
ajst-25583	97	40	subtilis	subtili	NOUN
ajst-25583	97	41	,	,	PUNCT
ajst-25583	97	42	frontiers	frontier	NOUN
ajst-25583	97	43	in	in	ADP
ajst-25583	97	44	microbiology	microbiology	NOUN
ajst-25583	97	45	,	,	PUNCT
ajst-25583	97	46	vol	vol	NOUN
ajst-25583	97	47	.	.	NOUN
ajst-25583	97	48	8	8	NUM
ajst-25583	97	49	(	(	PUNCT
ajst-25583	97	50	2017	2017	NUM
ajst-25583	97	51	)	)	PUNCT
ajst-25583	97	52	,	,	PUNCT
ajst-25583	97	53	p.1167	p.1167	NOUN
ajst-25583	97	54	-	-	PUNCT
ajst-25583	97	55	1178	1178	NUM
ajst-25583	97	56	.	.	PUNCT
ajst-25583	98	1	[	[	X
ajst-25583	98	2	34	34	NUM
ajst-25583	98	3	]	]	X
ajst-25583	98	4	d.	d.	PROPN
ajst-25583	98	5	y.	y.	PROPN
ajst-25583	98	6	liu	liu	PROPN
ajst-25583	98	7	,	,	PUNCT
ajst-25583	98	8	c.	c.	PROPN
ajst-25583	98	9	huang	huang	PROPN
ajst-25583	98	10	,	,	PUNCT
ajst-25583	98	11	j.	j.	PROPN
ajst-25583	98	12	x.	x.	PROPN
ajst-25583	98	13	guo	guo	PROPN
ajst-25583	98	14	,	,	PUNCT
ajst-25583	98	15	p.	p.	PROPN
ajst-25583	98	16	j.	j.	PROPN
ajst-25583	98	17	zhang	zhang	PROPN
ajst-25583	98	18	,	,	PUNCT
ajst-25583	98	19	t.	t.	PROPN
ajst-25583	98	20	chen	chen	PROPN
ajst-25583	98	21	,	,	PUNCT
ajst-25583	98	22	z.	z.	PROPN
ajst-25583	98	23	w.	w.	PROPN
ajst-25583	98	24	wang	wang	PROPN
ajst-25583	98	25	and	and	CCONJ
ajst-25583	98	26	x.	x.	PROPN
ajst-25583	98	27	m.	m.	PROPN
ajst-25583	98	28	zhao	zhao	PROPN
ajst-25583	98	29	:	:	PUNCT
ajst-25583	98	30	development	development	NOUN
ajst-25583	98	31	and	and	CCONJ
ajst-25583	98	32	characterization	characterization	NOUN
ajst-25583	98	33	of	of	ADP
ajst-25583	98	34	a	a	DET
ajst-25583	98	35	crispr	crispr	NOUN
ajst-25583	98	36	/	/	SYM
ajst-25583	98	37	cas9n	cas9n	VERB
ajst-25583	98	38	-	-	PUNCT
ajst-25583	98	39	based	base	VERB
ajst-25583	98	40	multiplex	multiplex	NOUN
ajst-25583	98	41	genome	genome	NOUN
ajst-25583	98	42	editing	editing	NOUN
ajst-25583	98	43	system	system	NOUN
ajst-25583	98	44	for	for	ADP
ajst-25583	98	45	bacillus	bacillus	NOUN
ajst-25583	98	46	subtilis	subtili	NOUN
ajst-25583	98	47	,	,	PUNCT
ajst-25583	98	48	biotechnology	biotechnology	NOUN
ajst-25583	98	49	for	for	ADP
ajst-25583	98	50	biofuels	biofuel	NOUN
ajst-25583	98	51	,	,	PUNCT
ajst-25583	98	52	vol	vol	NOUN
ajst-25583	98	53	.	.	PROPN
ajst-25583	98	54	12	12	NUM
ajst-25583	98	55	(	(	PUNCT
ajst-25583	98	56	2019	2019	NUM
ajst-25583	98	57	)	)	PUNCT
ajst-25583	98	58	no.1	no.1	NOUN
ajst-25583	98	59	,	,	PUNCT
ajst-25583	98	60	p.197	p.197	NOUN
ajst-25583	98	61	-	-	PUNCT
ajst-25583	98	62	213	213	NUM
ajst-25583	98	63	.	.	PUNCT