id	sid	tid	token	lemma	pos
ajst-25631	1	1	academic	academic	ADJ
ajst-25631	1	2	journal	journal	NOUN
ajst-25631	1	3	of	of	ADP
ajst-25631	1	4	science	science	NOUN
ajst-25631	1	5	and	and	CCONJ
ajst-25631	1	6	technology	technology	NOUN
ajst-25631	1	7	issn	issn	NOUN
ajst-25631	1	8	:	:	PUNCT
ajst-25631	1	9	2771	2771	NUM
ajst-25631	1	10	-	-	SYM
ajst-25631	1	11	3032	3032	NUM
ajst-25631	1	12	|	|	NOUN
ajst-25631	1	13	vol	vol	NOUN
ajst-25631	1	14	.	.	PROPN
ajst-25631	2	1	12	12	NUM
ajst-25631	2	2	,	,	PUNCT
ajst-25631	2	3	no	no	INTJ
ajst-25631	2	4	.	.	NOUN
ajst-25631	2	5	2	2	NUM
ajst-25631	2	6	,	,	PUNCT
ajst-25631	2	7	2024	2024	NUM
ajst-25631	2	8	223	223	NUM
ajst-25631	2	9	characterization	characterization	NOUN
ajst-25631	2	10	of	of	ADP
ajst-25631	2	11	escherichia	escherichia	NOUN
ajst-25631	2	12	coli	coli	NOUN
ajst-25631	2	13	as	as	ADP
ajst-25631	2	14	a	a	DET
ajst-25631	2	15	recombinant	recombinant	ADJ
ajst-25631	2	16	protein	protein	NOUN
ajst-25631	2	17	expression	expression	NOUN
ajst-25631	2	18	host	host	NOUN
ajst-25631	2	19	and	and	CCONJ
ajst-25631	2	20	its	its	PRON
ajst-25631	2	21	optimization	optimization	NOUN
ajst-25631	2	22	kang	kang	PROPN
ajst-25631	2	23	yu	yu	PROPN
ajst-25631	2	24	,	,	PUNCT
ajst-25631	2	25	congjing	congje	VERB
ajst-25631	2	26	cao	cao	PROPN
ajst-25631	2	27	,	,	PUNCT
ajst-25631	2	28	luwen	luwen	PROPN
ajst-25631	2	29	huang	huang	PROPN
ajst-25631	2	30	,	,	PUNCT
ajst-25631	2	31	enxu	enxu	PROPN
ajst-25631	2	32	wang	wang	PROPN
ajst-25631	2	33	*	*	PUNCT
ajst-25631	2	34	bloomage	bloomage	PROPN
ajst-25631	2	35	biotech	biotech	NOUN
ajst-25631	2	36	(	(	PUNCT
ajst-25631	2	37	tianjin	tianjin	PROPN
ajst-25631	2	38	)	)	PUNCT
ajst-25631	2	39	technology	technology	PROPN
ajst-25631	2	40	co.	co.	PROPN
ajst-25631	2	41	,	,	PUNCT
ajst-25631	2	42	ltd	ltd	PROPN
ajst-25631	2	43	,	,	PUNCT
ajst-25631	2	44	tianjin	tianjin	PROPN
ajst-25631	2	45	300000	300000	NUM
ajst-25631	2	46	,	,	PUNCT
ajst-25631	2	47	china	china	PROPN
ajst-25631	2	48	*	*	PUNCT
ajst-25631	2	49	corresponding	correspond	VERB
ajst-25631	2	50	author	author	NOUN
ajst-25631	2	51	:	:	PUNCT
ajst-25631	2	52	enxu	enxu	PROPN
ajst-25631	2	53	wang	wang	PROPN
ajst-25631	2	54	(	(	PUNCT
ajst-25631	2	55	email	email	NOUN
ajst-25631	2	56	:	:	PUNCT
ajst-25631	2	57	wangenxu@bloomagebiotech.com	wangenxu@bloomagebiotech.com	X
ajst-25631	2	58	)	)	PUNCT
ajst-25631	3	1	abstract	abstract	NOUN
ajst-25631	3	2	:	:	PUNCT
ajst-25631	3	3	engineered	engineer	VERB
ajst-25631	3	4	escherichia	escherichia	NOUN
ajst-25631	3	5	coli	coli	NOUN
ajst-25631	3	6	serves	serve	VERB
ajst-25631	3	7	as	as	ADP
ajst-25631	3	8	an	an	DET
ajst-25631	3	9	efficient	efficient	ADJ
ajst-25631	3	10	platform	platform	NOUN
ajst-25631	3	11	for	for	ADP
ajst-25631	3	12	the	the	DET
ajst-25631	3	13	production	production	NOUN
ajst-25631	3	14	of	of	ADP
ajst-25631	3	15	a	a	DET
ajst-25631	3	16	diverse	diverse	ADJ
ajst-25631	3	17	array	array	NOUN
ajst-25631	3	18	of	of	ADP
ajst-25631	3	19	recombinant	recombinant	ADJ
ajst-25631	3	20	proteins	protein	NOUN
ajst-25631	3	21	,	,	PUNCT
ajst-25631	3	22	thereby	thereby	ADV
ajst-25631	3	23	addressing	address	VERB
ajst-25631	3	24	the	the	DET
ajst-25631	3	25	pharmaceutical	pharmaceutical	ADJ
ajst-25631	3	26	industry	industry	NOUN
ajst-25631	3	27	's	's	PART
ajst-25631	3	28	demand	demand	NOUN
ajst-25631	3	29	for	for	ADP
ajst-25631	3	30	high	high	ADJ
ajst-25631	3	31	-	-	PUNCT
ajst-25631	3	32	quality	quality	NOUN
ajst-25631	3	33	biological	biological	ADJ
ajst-25631	3	34	agents	agent	NOUN
ajst-25631	3	35	.	.	PUNCT
ajst-25631	4	1	nevertheless	nevertheless	ADV
ajst-25631	4	2	,	,	PUNCT
ajst-25631	4	3	challenges	challenge	NOUN
ajst-25631	4	4	related	relate	VERB
ajst-25631	4	5	to	to	ADP
ajst-25631	4	6	protein	protein	NOUN
ajst-25631	4	7	folding	folding	NOUN
ajst-25631	4	8	and	and	CCONJ
ajst-25631	4	9	the	the	DET
ajst-25631	4	10	complexities	complexity	NOUN
ajst-25631	4	11	involved	involve	VERB
ajst-25631	4	12	in	in	ADP
ajst-25631	4	13	product	product	NOUN
ajst-25631	4	14	purification	purification	NOUN
ajst-25631	4	15	must	must	AUX
ajst-25631	4	16	be	be	AUX
ajst-25631	4	17	acknowledged	acknowledge	VERB
ajst-25631	4	18	.	.	PUNCT
ajst-25631	5	1	this	this	DET
ajst-25631	5	2	review	review	NOUN
ajst-25631	5	3	delineates	delineate	VERB
ajst-25631	5	4	the	the	DET
ajst-25631	5	5	advantages	advantage	NOUN
ajst-25631	5	6	and	and	CCONJ
ajst-25631	5	7	disadvantages	disadvantage	NOUN
ajst-25631	5	8	of	of	ADP
ajst-25631	5	9	utilizing	utilize	VERB
ajst-25631	5	10	e.	e.	PROPN
ajst-25631	5	11	coli	coli	NOUN
ajst-25631	5	12	as	as	ADP
ajst-25631	5	13	an	an	DET
ajst-25631	5	14	expression	expression	NOUN
ajst-25631	5	15	host	host	NOUN
ajst-25631	5	16	and	and	CCONJ
ajst-25631	5	17	further	far	ADV
ajst-25631	5	18	investigates	investigate	VERB
ajst-25631	5	19	various	various	ADJ
ajst-25631	5	20	strategies	strategy	NOUN
ajst-25631	5	21	aimed	aim	VERB
ajst-25631	5	22	at	at	ADP
ajst-25631	5	23	optimizing	optimize	VERB
ajst-25631	5	24	and	and	CCONJ
ajst-25631	5	25	enhancing	enhance	VERB
ajst-25631	5	26	the	the	DET
ajst-25631	5	27	e.	e.	PROPN
ajst-25631	5	28	coli	coli	NOUN
ajst-25631	5	29	expression	expression	NOUN
ajst-25631	5	30	system	system	NOUN
ajst-25631	5	31	to	to	PART
ajst-25631	5	32	elevate	elevate	VERB
ajst-25631	5	33	the	the	DET
ajst-25631	5	34	yield	yield	NOUN
ajst-25631	5	35	,	,	PUNCT
ajst-25631	5	36	purity	purity	NOUN
ajst-25631	5	37	,	,	PUNCT
ajst-25631	5	38	and	and	CCONJ
ajst-25631	5	39	biological	biological	ADJ
ajst-25631	5	40	activity	activity	NOUN
ajst-25631	5	41	of	of	ADP
ajst-25631	5	42	recombinant	recombinant	ADJ
ajst-25631	5	43	proteins	protein	NOUN
ajst-25631	5	44	,	,	PUNCT
ajst-25631	5	45	ultimately	ultimately	ADV
ajst-25631	5	46	supporting	support	VERB
ajst-25631	5	47	advancements	advancement	NOUN
ajst-25631	5	48	in	in	ADP
ajst-25631	5	49	biopharmaceuticals	biopharmaceutical	NOUN
ajst-25631	5	50	and	and	CCONJ
ajst-25631	5	51	other	other	ADJ
ajst-25631	5	52	relevant	relevant	ADJ
ajst-25631	5	53	fields	field	NOUN
ajst-25631	5	54	.	.	PUNCT
ajst-25631	6	1	keywords	keyword	NOUN
ajst-25631	6	2	:	:	PUNCT
ajst-25631	6	3	escherichia	escherichia	NOUN
ajst-25631	6	4	coli	coli	NOUN
ajst-25631	6	5	;	;	PUNCT
ajst-25631	6	6	recombinant	recombinant	ADJ
ajst-25631	6	7	protein	protein	NOUN
ajst-25631	6	8	;	;	PUNCT
ajst-25631	6	9	expression	expression	NOUN
ajst-25631	6	10	host	host	NOUN
ajst-25631	6	11	;	;	PUNCT
ajst-25631	6	12	optimization	optimization	NOUN
ajst-25631	6	13	strategy	strategy	NOUN
ajst-25631	6	14	.	.	PUNCT
ajst-25631	7	1	1	1	X
ajst-25631	7	2	.	.	X
ajst-25631	7	3	introduction	introduction	NOUN
ajst-25631	7	4	with	with	ADP
ajst-25631	7	5	the	the	DET
ajst-25631	7	6	rapid	rapid	ADJ
ajst-25631	7	7	development	development	NOUN
ajst-25631	7	8	of	of	ADP
ajst-25631	7	9	molecular	molecular	ADJ
ajst-25631	7	10	biology	biology	NOUN
ajst-25631	7	11	and	and	CCONJ
ajst-25631	7	12	proteomics	proteomic	NOUN
ajst-25631	7	13	,	,	PUNCT
ajst-25631	7	14	the	the	DET
ajst-25631	7	15	genetic	genetic	ADJ
ajst-25631	7	16	manipulation	manipulation	NOUN
ajst-25631	7	17	technology	technology	NOUN
ajst-25631	7	18	of	of	ADP
ajst-25631	7	19	exogenous	exogenous	ADJ
ajst-25631	7	20	gene	gene	NOUN
ajst-25631	7	21	expression	expression	NOUN
ajst-25631	7	22	is	be	AUX
ajst-25631	7	23	becoming	become	VERB
ajst-25631	7	24	more	more	ADV
ajst-25631	7	25	and	and	CCONJ
ajst-25631	7	26	more	more	ADV
ajst-25631	7	27	mature	mature	ADJ
ajst-25631	7	28	.	.	PUNCT
ajst-25631	8	1	expression	expression	NOUN
ajst-25631	8	2	system	system	NOUN
ajst-25631	8	3	is	be	AUX
ajst-25631	8	4	the	the	DET
ajst-25631	8	5	core	core	NOUN
ajst-25631	8	6	of	of	ADP
ajst-25631	8	7	exogenous	exogenous	ADJ
ajst-25631	8	8	gene	gene	NOUN
ajst-25631	8	9	expression	expression	NOUN
ajst-25631	8	10	,	,	PUNCT
ajst-25631	8	11	and	and	CCONJ
ajst-25631	8	12	commonly	commonly	ADV
ajst-25631	8	13	used	use	VERB
ajst-25631	8	14	expression	expression	NOUN
ajst-25631	8	15	systems	system	NOUN
ajst-25631	8	16	are	be	AUX
ajst-25631	8	17	generally	generally	ADV
ajst-25631	8	18	model	model	ADJ
ajst-25631	8	19	organisms	organism	NOUN
ajst-25631	8	20	,	,	PUNCT
ajst-25631	8	21	including	include	VERB
ajst-25631	8	22	eukaryotic	eukaryotic	ADJ
ajst-25631	8	23	and	and	CCONJ
ajst-25631	8	24	prokaryotic	prokaryotic	ADJ
ajst-25631	8	25	expression	expression	NOUN
ajst-25631	8	26	systems	system	NOUN
ajst-25631	8	27	,	,	PUNCT
ajst-25631	8	28	among	among	ADP
ajst-25631	8	29	which	which	PRON
ajst-25631	8	30	eukaryotic	eukaryotic	ADJ
ajst-25631	8	31	systems	system	NOUN
ajst-25631	8	32	include	include	VERB
ajst-25631	8	33	mammalian	mammalian	ADJ
ajst-25631	8	34	cell	cell	NOUN
ajst-25631	8	35	expression	expression	NOUN
ajst-25631	8	36	system	system	NOUN
ajst-25631	8	37	,	,	PUNCT
ajst-25631	8	38	plant	plant	NOUN
ajst-25631	8	39	expression	expression	NOUN
ajst-25631	8	40	system	system	NOUN
ajst-25631	8	41	,	,	PUNCT
ajst-25631	8	42	insect	insect	NOUN
ajst-25631	8	43	baculovirus	baculovirus	NOUN
ajst-25631	8	44	expression	expression	NOUN
ajst-25631	8	45	vector	vector	NOUN
ajst-25631	8	46	system	system	NOUN
ajst-25631	8	47	and	and	CCONJ
ajst-25631	8	48	yeast	yeast	NOUN
ajst-25631	8	49	expression	expression	NOUN
ajst-25631	8	50	system	system	NOUN
ajst-25631	8	51	,	,	PUNCT
ajst-25631	8	52	and	and	CCONJ
ajst-25631	8	53	prokaryotic	prokaryotic	ADJ
ajst-25631	8	54	expression	expression	NOUN
ajst-25631	8	55	system	system	NOUN
ajst-25631	8	56	is	be	AUX
ajst-25631	8	57	mainly	mainly	ADV
ajst-25631	8	58	e.	e.	PROPN
ajst-25631	8	59	coli	coli	NOUN
ajst-25631	8	60	expression	expression	NOUN
ajst-25631	8	61	system	system	NOUN
ajst-25631	8	62	.	.	PUNCT
ajst-25631	9	1	e.	e.	PROPN
ajst-25631	9	2	coli	coli	PROPN
ajst-25631	9	3	is	be	AUX
ajst-25631	9	4	one	one	NUM
ajst-25631	9	5	of	of	ADP
ajst-25631	9	6	the	the	DET
ajst-25631	9	7	most	most	ADV
ajst-25631	9	8	widely	widely	ADV
ajst-25631	9	9	used	use	VERB
ajst-25631	9	10	prokaryotic	prokaryotic	ADJ
ajst-25631	9	11	expression	expression	NOUN
ajst-25631	9	12	system	system	NOUN
ajst-25631	9	13	and	and	CCONJ
ajst-25631	9	14	the	the	DET
ajst-25631	9	15	earliest	early	ADJ
ajst-25631	9	16	exogenous	exogenous	ADJ
ajst-25631	9	17	gene	gene	NOUN
ajst-25631	9	18	expression	expression	NOUN
ajst-25631	9	19	system	system	NOUN
ajst-25631	9	20	to	to	PART
ajst-25631	9	21	be	be	AUX
ajst-25631	9	22	studied	study	VERB
ajst-25631	9	23	,	,	PUNCT
ajst-25631	9	24	with	with	ADP
ajst-25631	9	25	a	a	DET
ajst-25631	9	26	clear	clear	ADJ
ajst-25631	9	27	genetic	genetic	ADJ
ajst-25631	9	28	background	background	NOUN
ajst-25631	9	29	,	,	PUNCT
ajst-25631	9	30	fast	fast	ADJ
ajst-25631	9	31	growth	growth	NOUN
ajst-25631	9	32	,	,	PUNCT
ajst-25631	9	33	easy	easy	ADJ
ajst-25631	9	34	to	to	PART
ajst-25631	9	35	achieve	achieve	VERB
ajst-25631	9	36	high	high	ADJ
ajst-25631	9	37	density	density	NOUN
ajst-25631	9	38	cultivation	cultivation	NOUN
ajst-25631	9	39	,	,	PUNCT
ajst-25631	9	40	low	low	ADJ
ajst-25631	9	41	cost	cost	NOUN
ajst-25631	9	42	,	,	PUNCT
ajst-25631	9	43	high	high	ADJ
ajst-25631	9	44	yield	yield	NOUN
ajst-25631	9	45	and	and	CCONJ
ajst-25631	9	46	incomparable	incomparable	ADJ
ajst-25631	9	47	superiority	superiority	NOUN
ajst-25631	9	48	compared	compare	VERB
ajst-25631	9	49	with	with	ADP
ajst-25631	9	50	other	other	ADJ
ajst-25631	9	51	expression	expression	NOUN
ajst-25631	9	52	systems	system	NOUN
ajst-25631	9	53	,	,	PUNCT
ajst-25631	9	54	it	it	PRON
ajst-25631	9	55	is	be	AUX
ajst-25631	9	56	the	the	DET
ajst-25631	9	57	most	most	ADV
ajst-25631	9	58	widely	widely	ADV
ajst-25631	9	59	used	use	VERB
ajst-25631	9	60	expression	expression	NOUN
ajst-25631	9	61	system	system	NOUN
ajst-25631	9	62	in	in	ADP
ajst-25631	9	63	commercial	commercial	ADJ
ajst-25631	9	64	production	production	NOUN
ajst-25631	9	65	,	,	PUNCT
ajst-25631	9	66	and	and	CCONJ
ajst-25631	9	67	it	it	PRON
ajst-25631	9	68	has	have	AUX
ajst-25631	9	69	achieved	achieve	VERB
ajst-25631	9	70	great	great	ADJ
ajst-25631	9	71	scientific	scientific	ADJ
ajst-25631	9	72	research	research	NOUN
ajst-25631	9	73	value	value	NOUN
ajst-25631	9	74	and	and	CCONJ
ajst-25631	9	75	economic	economic	ADJ
ajst-25631	9	76	benefits	benefit	NOUN
ajst-25631	9	77	[	[	X
ajst-25631	9	78	1	1	NUM
ajst-25631	9	79	]	]	PUNCT
ajst-25631	9	80	.	.	PUNCT
ajst-25631	10	1	the	the	DET
ajst-25631	10	2	e.	e.	PROPN
ajst-25631	10	3	coli	coli	NOUN
ajst-25631	10	4	expression	expression	NOUN
ajst-25631	10	5	system	system	NOUN
ajst-25631	10	6	is	be	AUX
ajst-25631	10	7	now	now	ADV
ajst-25631	10	8	widely	widely	ADV
ajst-25631	10	9	used	use	VERB
ajst-25631	10	10	for	for	ADP
ajst-25631	10	11	the	the	DET
ajst-25631	10	12	expression	expression	NOUN
ajst-25631	10	13	production	production	NOUN
ajst-25631	10	14	of	of	ADP
ajst-25631	10	15	a	a	DET
ajst-25631	10	16	variety	variety	NOUN
ajst-25631	10	17	of	of	ADP
ajst-25631	10	18	protein	protein	NOUN
ajst-25631	10	19	/	/	SYM
ajst-25631	10	20	peptide	peptide	NOUN
ajst-25631	10	21	drugs	drug	NOUN
ajst-25631	10	22	and	and	CCONJ
ajst-25631	10	23	biochemicals	biochemical	NOUN
ajst-25631	10	24	,	,	PUNCT
ajst-25631	10	25	including	include	VERB
ajst-25631	10	26	:	:	PUNCT
ajst-25631	10	27	recombinant	recombinant	ADJ
ajst-25631	10	28	human	human	ADJ
ajst-25631	10	29	insulin	insulin	NOUN
ajst-25631	10	30	,	,	PUNCT
ajst-25631	10	31	a2b	a2b	NOUN
ajst-25631	10	32	-	-	PUNCT
ajst-25631	10	33	type	type	NOUN
ajst-25631	10	34	interferon	interferon	NOUN
ajst-25631	10	35	,	,	PUNCT
ajst-25631	10	36	ranibizumab	ranibizumab	NOUN
ajst-25631	10	37	,	,	PUNCT
ajst-25631	10	38	purple	purple	ADJ
ajst-25631	10	39	bacillin	bacillin	NOUN
ajst-25631	10	40	and	and	CCONJ
ajst-25631	10	41	peony	peony	NOUN
ajst-25631	10	42	skin	skin	NOUN
ajst-25631	10	43	glucoside[2	glucoside[2	PROPN
ajst-25631	10	44	]	]	PUNCT
ajst-25631	10	45	.	.	PUNCT
ajst-25631	11	1	according	accord	VERB
ajst-25631	11	2	to	to	ADP
ajst-25631	11	3	statistics	statistic	NOUN
ajst-25631	11	4	,	,	PUNCT
ajst-25631	11	5	26	26	NUM
ajst-25631	11	6	%	%	NOUN
ajst-25631	11	7	of	of	ADP
ajst-25631	11	8	recombinant	recombinant	ADJ
ajst-25631	11	9	protein	protein	NOUN
ajst-25631	11	10	drugs	drug	NOUN
ajst-25631	11	11	approved	approve	VERB
ajst-25631	11	12	for	for	ADP
ajst-25631	11	13	marketing	marketing	NOUN
ajst-25631	11	14	by	by	ADP
ajst-25631	11	15	the	the	DET
ajst-25631	11	16	u.s	u.s	PROPN
ajst-25631	11	17	.	.	PROPN
ajst-25631	11	18	fda	fda	PROPN
ajst-25631	11	19	and	and	CCONJ
ajst-25631	11	20	the	the	DET
ajst-25631	11	21	european	european	PROPN
ajst-25631	11	22	ema	ema	PROPN
ajst-25631	11	23	from	from	ADP
ajst-25631	11	24	1986	1986	NUM
ajst-25631	11	25	-	-	SYM
ajst-25631	11	26	2018	2018	NUM
ajst-25631	11	27	came	come	VERB
ajst-25631	11	28	from	from	ADP
ajst-25631	11	29	e.	e.	PROPN
ajst-25631	11	30	coli	coli	PROPN
ajst-25631	11	31	.	.	PUNCT
ajst-25631	12	1	meanwhile	meanwhile	ADV
ajst-25631	12	2	,	,	PUNCT
ajst-25631	12	3	genetically	genetically	ADV
ajst-25631	12	4	engineered	engineer	VERB
ajst-25631	12	5	vaccines	vaccine	NOUN
ajst-25631	12	6	expressed	express	VERB
ajst-25631	12	7	through	through	ADP
ajst-25631	12	8	e.	e.	PROPN
ajst-25631	12	9	coli	coli	PROPN
ajst-25631	12	10	are	be	AUX
ajst-25631	12	11	now	now	ADV
ajst-25631	12	12	entering	enter	VERB
ajst-25631	12	13	the	the	DET
ajst-25631	12	14	market	market	NOUN
ajst-25631	12	15	or	or	CCONJ
ajst-25631	12	16	in	in	ADP
ajst-25631	12	17	the	the	DET
ajst-25631	12	18	clinical	clinical	ADJ
ajst-25631	12	19	trial	trial	NOUN
ajst-25631	12	20	stage	stage	NOUN
ajst-25631	12	21	,	,	PUNCT
ajst-25631	12	22	such	such	ADJ
ajst-25631	12	23	as	as	ADP
ajst-25631	12	24	hepatitis	hepatitis	NOUN
ajst-25631	12	25	e	e	NOUN
ajst-25631	12	26	vaccine	vaccine	NOUN
ajst-25631	12	27	,	,	PUNCT
ajst-25631	12	28	human	human	ADJ
ajst-25631	12	29	papillomavirus	papillomavirus	NOUN
ajst-25631	12	30	vaccine	vaccine	NOUN
ajst-25631	12	31	,	,	PUNCT
ajst-25631	12	32	influenza	influenza	VERB
ajst-25631	12	33	a	a	DET
ajst-25631	12	34	vaccine	vaccine	NOUN
ajst-25631	12	35	and	and	CCONJ
ajst-25631	12	36	so	so	ADV
ajst-25631	12	37	on	on	ADV
ajst-25631	12	38	.	.	PUNCT
ajst-25631	13	1	common	common	ADJ
ajst-25631	13	2	e.	e.	PROPN
ajst-25631	13	3	coli	coli	NOUN
ajst-25631	13	4	expression	expression	NOUN
ajst-25631	13	5	systems	system	NOUN
ajst-25631	13	6	include	include	VERB
ajst-25631	13	7	bl21	bl21	PROPN
ajst-25631	13	8	series	series	NOUN
ajst-25631	13	9	,	,	PUNCT
ajst-25631	13	10	jm109	jm109	PROPN
ajst-25631	13	11	series	series	NOUN
ajst-25631	13	12	,	,	PUNCT
ajst-25631	13	13	w3110	w3110	PROPN
ajst-25631	13	14	series	series	NOUN
ajst-25631	13	15	and	and	CCONJ
ajst-25631	13	16	k802	k802	PROPN
ajst-25631	13	17	series	series	NOUN
ajst-25631	13	18	,	,	PUNCT
ajst-25631	13	19	etc	etc	X
ajst-25631	13	20	.	.	X
ajst-25631	14	1	among	among	ADP
ajst-25631	14	2	them	they	PRON
ajst-25631	14	3	,	,	PUNCT
ajst-25631	14	4	e.	e.	PROPN
ajst-25631	14	5	coli	coli	NOUN
ajst-25631	15	1	bl21	bl21	PROPN
ajst-25631	15	2	(	(	PUNCT
ajst-25631	15	3	de3	de3	NOUN
ajst-25631	15	4	)	)	PUNCT
ajst-25631	15	5	strain	strain	NOUN
ajst-25631	15	6	is	be	AUX
ajst-25631	15	7	one	one	NUM
ajst-25631	15	8	of	of	ADP
ajst-25631	15	9	the	the	DET
ajst-25631	15	10	most	most	ADV
ajst-25631	15	11	widely	widely	ADV
ajst-25631	15	12	used	use	VERB
ajst-25631	15	13	strains	strain	NOUN
ajst-25631	15	14	in	in	ADP
ajst-25631	15	15	recombinant	recombinant	ADJ
ajst-25631	15	16	protein	protein	NOUN
ajst-25631	15	17	expression	expression	NOUN
ajst-25631	15	18	research	research	NOUN
ajst-25631	15	19	,	,	PUNCT
ajst-25631	16	1	bl21	bl21	PROPN
ajst-25631	16	2	(	(	PUNCT
ajst-25631	16	3	de3	de3	PROPN
ajst-25631	16	4	)	)	PUNCT
ajst-25631	16	5	is	be	AUX
ajst-25631	16	6	obtained	obtain	VERB
ajst-25631	16	7	from	from	ADP
ajst-25631	16	8	the	the	DET
ajst-25631	16	9	derivatives	derivative	NOUN
ajst-25631	16	10	of	of	ADP
ajst-25631	16	11	e.	e.	PROPN
ajst-25631	16	12	coli	coli	PROPN
ajst-25631	16	13	b	b	PROPN
ajst-25631	16	14	series	series	NOUN
ajst-25631	16	15	and	and	CCONJ
ajst-25631	16	16	k-12	k-12	PROPN
ajst-25631	16	17	series	series	PROPN
ajst-25631	16	18	through	through	ADP
ajst-25631	16	19	genetic	genetic	ADJ
ajst-25631	16	20	mutations	mutation	NOUN
ajst-25631	16	21	such	such	ADJ
ajst-25631	16	22	as	as	ADP
ajst-25631	16	23	p1	p1	NOUN
ajst-25631	16	24	transduction	transduction	NOUN
ajst-25631	16	25	.	.	PUNCT
ajst-25631	17	1	these	these	DET
ajst-25631	17	2	strains	strain	NOUN
ajst-25631	17	3	are	be	AUX
ajst-25631	17	4	usually	usually	ADV
ajst-25631	17	5	host	host	NOUN
ajst-25631	17	6	protease	protease	NOUN
ajst-25631	17	7	-	-	PUNCT
ajst-25631	17	8	deficient	deficient	NOUN
ajst-25631	17	9	to	to	PART
ajst-25631	17	10	ensure	ensure	VERB
ajst-25631	17	11	that	that	SCONJ
ajst-25631	17	12	the	the	DET
ajst-25631	17	13	exogenous	exogenous	ADJ
ajst-25631	17	14	proteins	protein	NOUN
ajst-25631	17	15	are	be	AUX
ajst-25631	17	16	not	not	PART
ajst-25631	17	17	degraded	degrade	VERB
ajst-25631	17	18	during	during	ADP
ajst-25631	17	19	the	the	DET
ajst-25631	17	20	expression	expression	NOUN
ajst-25631	17	21	process	process	NOUN
ajst-25631	17	22	and	and	CCONJ
ajst-25631	17	23	to	to	PART
ajst-25631	17	24	maintain	maintain	VERB
ajst-25631	17	25	the	the	DET
ajst-25631	17	26	stability	stability	NOUN
ajst-25631	17	27	of	of	ADP
ajst-25631	17	28	expression	expression	NOUN
ajst-25631	17	29	[	[	X
ajst-25631	17	30	1,2	1,2	NUM
ajst-25631	17	31	]	]	PUNCT
ajst-25631	17	32	.	.	PUNCT
ajst-25631	18	1	2	2	X
ajst-25631	18	2	.	.	X
ajst-25631	18	3	advantage	advantage	NOUN
ajst-25631	18	4	of	of	ADP
ajst-25631	18	5	escherichia	escherichia	NOUN
ajst-25631	18	6	coli	coli	NOUN
ajst-25631	18	7	as	as	ADP
ajst-25631	18	8	an	an	DET
ajst-25631	18	9	expression	expression	NOUN
ajst-25631	18	10	host	host	NOUN
ajst-25631	18	11	as	as	ADP
ajst-25631	18	12	an	an	DET
ajst-25631	18	13	expression	expression	NOUN
ajst-25631	18	14	host	host	NOUN
ajst-25631	18	15	,	,	PUNCT
ajst-25631	18	16	escherichia	escherichia	NOUN
ajst-25631	18	17	coli	coli	NOUN
ajst-25631	18	18	has	have	AUX
ajst-25631	18	19	been	be	AUX
ajst-25631	18	20	widely	widely	ADV
ajst-25631	18	21	used	use	VERB
ajst-25631	18	22	in	in	ADP
ajst-25631	18	23	the	the	DET
ajst-25631	18	24	field	field	NOUN
ajst-25631	18	25	of	of	ADP
ajst-25631	18	26	biopharmaceutical	biopharmaceutical	NOUN
ajst-25631	18	27	because	because	SCONJ
ajst-25631	18	28	of	of	ADP
ajst-25631	18	29	its	its	PRON
ajst-25631	18	30	many	many	ADJ
ajst-25631	18	31	advantages	advantage	NOUN
ajst-25631	18	32	.	.	PUNCT
ajst-25631	19	1	the	the	DET
ajst-25631	19	2	study	study	NOUN
ajst-25631	19	3	of	of	ADP
ajst-25631	19	4	escherichia	escherichia	NOUN
ajst-25631	19	5	coli	coli	NOUN
ajst-25631	19	6	has	have	VERB
ajst-25631	19	7	a	a	DET
ajst-25631	19	8	long	long	ADJ
ajst-25631	19	9	history	history	NOUN
ajst-25631	19	10	,	,	PUNCT
ajst-25631	19	11	with	with	ADP
ajst-25631	19	12	a	a	DET
ajst-25631	19	13	lot	lot	NOUN
ajst-25631	19	14	of	of	ADP
ajst-25631	19	15	data	datum	NOUN
ajst-25631	19	16	and	and	CCONJ
ajst-25631	19	17	experience	experience	NOUN
ajst-25631	19	18	accumulation	accumulation	NOUN
ajst-25631	19	19	.	.	PUNCT
ajst-25631	20	1	at	at	ADP
ajst-25631	20	2	the	the	DET
ajst-25631	20	3	same	same	ADJ
ajst-25631	20	4	time	time	NOUN
ajst-25631	20	5	,	,	PUNCT
ajst-25631	20	6	its	its	PRON
ajst-25631	20	7	genetic	genetic	ADJ
ajst-25631	20	8	background	background	NOUN
ajst-25631	20	9	is	be	AUX
ajst-25631	20	10	quite	quite	ADV
ajst-25631	20	11	clear	clear	ADJ
ajst-25631	20	12	,	,	PUNCT
ajst-25631	20	13	and	and	CCONJ
ajst-25631	20	14	researchers	researcher	NOUN
ajst-25631	20	15	have	have	VERB
ajst-25631	20	16	a	a	DET
ajst-25631	20	17	very	very	ADJ
ajst-25631	20	18	in	in	ADP
ajst-25631	20	19	-	-	PUNCT
ajst-25631	20	20	depth	depth	NOUN
ajst-25631	20	21	understanding	understanding	NOUN
ajst-25631	20	22	of	of	ADP
ajst-25631	20	23	the	the	DET
ajst-25631	20	24	e.	e.	PROPN
ajst-25631	20	25	coli	coli	NOUN
ajst-25631	20	26	genome	genome	NOUN
ajst-25631	20	27	.	.	PUNCT
ajst-25631	21	1	this	this	PRON
ajst-25631	21	2	allows	allow	VERB
ajst-25631	21	3	scientists	scientist	NOUN
ajst-25631	21	4	to	to	PART
ajst-25631	21	5	easily	easily	ADV
ajst-25631	21	6	perform	perform	VERB
ajst-25631	21	7	gene	gene	NOUN
ajst-25631	21	8	cloning	cloning	NOUN
ajst-25631	21	9	,	,	PUNCT
ajst-25631	21	10	expression	expression	NOUN
ajst-25631	21	11	regulation	regulation	NOUN
ajst-25631	21	12	,	,	PUNCT
ajst-25631	21	13	and	and	CCONJ
ajst-25631	21	14	genetic	genetic	ADJ
ajst-25631	21	15	engineering	engineering	NOUN
ajst-25631	21	16	operations	operation	NOUN
ajst-25631	21	17	.	.	PUNCT
ajst-25631	22	1	by	by	ADP
ajst-25631	22	2	using	use	VERB
ajst-25631	22	3	multiple	multiple	ADJ
ajst-25631	22	4	vectors	vector	NOUN
ajst-25631	22	5	and	and	CCONJ
ajst-25631	22	6	expression	expression	NOUN
ajst-25631	22	7	systems	system	NOUN
ajst-25631	22	8	,	,	PUNCT
ajst-25631	22	9	e.	e.	PROPN
ajst-25631	22	10	coli	coli	PROPN
ajst-25631	22	11	can	can	AUX
ajst-25631	22	12	effectively	effectively	ADV
ajst-25631	22	13	express	express	VERB
ajst-25631	22	14	foreign	foreign	ADJ
ajst-25631	22	15	genes	gene	NOUN
ajst-25631	22	16	and	and	CCONJ
ajst-25631	22	17	produce	produce	VERB
ajst-25631	22	18	target	target	NOUN
ajst-25631	22	19	proteins	protein	NOUN
ajst-25631	22	20	[	[	X
ajst-25631	22	21	3,4	3,4	NUM
ajst-25631	22	22	]	]	PUNCT
ajst-25631	22	23	.	.	PUNCT
ajst-25631	23	1	due	due	ADP
ajst-25631	23	2	to	to	ADP
ajst-25631	23	3	the	the	DET
ajst-25631	23	4	widespread	widespread	ADJ
ajst-25631	23	5	use	use	NOUN
ajst-25631	23	6	of	of	ADP
ajst-25631	23	7	e.	e.	PROPN
ajst-25631	23	8	coli	coli	NOUN
ajst-25631	23	9	in	in	ADP
ajst-25631	23	10	recombinant	recombinant	ADJ
ajst-25631	23	11	protein	protein	NOUN
ajst-25631	23	12	production	production	NOUN
ajst-25631	23	13	,	,	PUNCT
ajst-25631	23	14	a	a	DET
ajst-25631	23	15	large	large	ADJ
ajst-25631	23	16	number	number	NOUN
ajst-25631	23	17	of	of	ADP
ajst-25631	23	18	molecular	molecular	ADJ
ajst-25631	23	19	biology	biology	NOUN
ajst-25631	23	20	tools	tool	NOUN
ajst-25631	23	21	and	and	CCONJ
ajst-25631	23	22	techniques	technique	NOUN
ajst-25631	23	23	have	have	AUX
ajst-25631	23	24	been	be	AUX
ajst-25631	23	25	established	establish	VERB
ajst-25631	23	26	.	.	PUNCT
ajst-25631	24	1	researchers	researcher	NOUN
ajst-25631	24	2	can	can	AUX
ajst-25631	24	3	utilize	utilize	VERB
ajst-25631	24	4	a	a	DET
ajst-25631	24	5	wealth	wealth	NOUN
ajst-25631	24	6	of	of	ADP
ajst-25631	24	7	expression	expression	NOUN
ajst-25631	24	8	plasmids	plasmid	NOUN
ajst-25631	24	9	,	,	PUNCT
ajst-25631	24	10	promoters	promoter	NOUN
ajst-25631	24	11	,	,	PUNCT
ajst-25631	24	12	screening	screen	VERB
ajst-25631	24	13	markers	marker	NOUN
ajst-25631	24	14	,	,	PUNCT
ajst-25631	24	15	and	and	CCONJ
ajst-25631	24	16	culture	culture	NOUN
ajst-25631	24	17	conditions	condition	NOUN
ajst-25631	24	18	to	to	PART
ajst-25631	24	19	optimize	optimize	VERB
ajst-25631	24	20	the	the	DET
ajst-25631	24	21	production	production	NOUN
ajst-25631	24	22	of	of	ADP
ajst-25631	24	23	target	target	NOUN
ajst-25631	24	24	proteins	protein	NOUN
ajst-25631	24	25	.	.	PUNCT
ajst-25631	25	1	the	the	DET
ajst-25631	25	2	availability	availability	NOUN
ajst-25631	25	3	of	of	ADP
ajst-25631	25	4	these	these	DET
ajst-25631	25	5	technologies	technology	NOUN
ajst-25631	25	6	allows	allow	VERB
ajst-25631	25	7	researchers	researcher	NOUN
ajst-25631	25	8	to	to	PART
ajst-25631	25	9	more	more	ADV
ajst-25631	25	10	effectively	effectively	ADV
ajst-25631	25	11	address	address	VERB
ajst-25631	25	12	the	the	DET
ajst-25631	25	13	various	various	ADJ
ajst-25631	25	14	challenges	challenge	NOUN
ajst-25631	25	15	encountered	encounter	VERB
ajst-25631	25	16	during	during	ADP
ajst-25631	25	17	protein	protein	NOUN
ajst-25631	25	18	expression	expression	NOUN
ajst-25631	25	19	[	[	X
ajst-25631	25	20	5,6	5,6	NUM
ajst-25631	25	21	]	]	PUNCT
ajst-25631	25	22	.	.	PUNCT
ajst-25631	26	1	e.	e.	PROPN
ajst-25631	26	2	coli	coli	PROPN
ajst-25631	26	3	is	be	AUX
ajst-25631	26	4	good	good	ADJ
ajst-25631	26	5	at	at	ADP
ajst-25631	26	6	producing	produce	VERB
ajst-25631	26	7	non	non	ADJ
ajst-25631	26	8	-	-	ADJ
ajst-25631	26	9	glycosylated	glycosylated	ADJ
ajst-25631	26	10	proteins	protein	NOUN
ajst-25631	26	11	,	,	PUNCT
ajst-25631	26	12	which	which	PRON
ajst-25631	26	13	is	be	AUX
ajst-25631	26	14	particularly	particularly	ADV
ajst-25631	26	15	important	important	ADJ
ajst-25631	26	16	for	for	ADP
ajst-25631	26	17	many	many	ADJ
ajst-25631	26	18	biotechnology	biotechnology	NOUN
ajst-25631	26	19	applications	application	NOUN
ajst-25631	26	20	.	.	PUNCT
ajst-25631	27	1	given	give	VERB
ajst-25631	27	2	the	the	DET
ajst-25631	27	3	complexity	complexity	NOUN
ajst-25631	27	4	of	of	ADP
ajst-25631	27	5	mammalian	mammalian	ADJ
ajst-25631	27	6	cell	cell	NOUN
ajst-25631	27	7	systems	system	NOUN
ajst-25631	27	8	in	in	ADP
ajst-25631	27	9	glycation	glycation	NOUN
ajst-25631	27	10	modification	modification	NOUN
ajst-25631	27	11	,	,	PUNCT
ajst-25631	27	12	many	many	ADJ
ajst-25631	27	13	researchers	researcher	NOUN
ajst-25631	27	14	have	have	AUX
ajst-25631	27	15	chosen	choose	VERB
ajst-25631	27	16	to	to	PART
ajst-25631	27	17	use	use	VERB
ajst-25631	27	18	e.	e.	PROPN
ajst-25631	27	19	coli	coli	NOUN
ajst-25631	27	20	to	to	PART
ajst-25631	27	21	produce	produce	VERB
ajst-25631	27	22	recombinant	recombinant	ADJ
ajst-25631	27	23	proteins	protein	NOUN
ajst-25631	27	24	that	that	PRON
ajst-25631	27	25	do	do	AUX
ajst-25631	27	26	not	not	PART
ajst-25631	27	27	require	require	VERB
ajst-25631	27	28	glycation	glycation	NOUN
ajst-25631	27	29	,	,	PUNCT
ajst-25631	27	30	thereby	thereby	ADV
ajst-25631	27	31	avoiding	avoid	VERB
ajst-25631	27	32	the	the	DET
ajst-25631	27	33	associated	associated	ADJ
ajst-25631	27	34	complexity	complexity	NOUN
ajst-25631	27	35	and	and	CCONJ
ajst-25631	27	36	cost	cost	VERB
ajst-25631	27	37	[	[	X
ajst-25631	27	38	7,8	7,8	NUM
ajst-25631	27	39	]	]	PUNCT
ajst-25631	27	40	.	.	PUNCT
ajst-25631	28	1	escherichia	escherichia	NOUN
ajst-25631	28	2	coli	coli	NOUN
ajst-25631	28	3	has	have	VERB
ajst-25631	28	4	a	a	DET
ajst-25631	28	5	very	very	ADV
ajst-25631	28	6	fast	fast	ADJ
ajst-25631	28	7	growth	growth	NOUN
ajst-25631	28	8	rate	rate	NOUN
ajst-25631	28	9	,	,	PUNCT
ajst-25631	28	10	and	and	CCONJ
ajst-25631	28	11	this	this	DET
ajst-25631	28	12	high	high	ADJ
ajst-25631	28	13	growth	growth	NOUN
ajst-25631	28	14	rate	rate	NOUN
ajst-25631	28	15	enables	enable	VERB
ajst-25631	28	16	escherichia	escherichia	NOUN
ajst-25631	28	17	coli	coli	NOUN
ajst-25631	28	18	to	to	PART
ajst-25631	28	19	reach	reach	VERB
ajst-25631	28	20	a	a	DET
ajst-25631	28	21	high	high	ADJ
ajst-25631	28	22	density	density	NOUN
ajst-25631	28	23	of	of	ADP
ajst-25631	28	24	cells	cell	NOUN
ajst-25631	28	25	in	in	ADP
ajst-25631	28	26	a	a	DET
ajst-25631	28	27	short	short	ADJ
ajst-25631	28	28	time	time	NOUN
ajst-25631	28	29	,	,	PUNCT
ajst-25631	28	30	thereby	thereby	ADV
ajst-25631	28	31	rapidly	rapidly	ADV
ajst-25631	28	32	producing	produce	VERB
ajst-25631	28	33	a	a	DET
ajst-25631	28	34	large	large	ADJ
ajst-25631	28	35	number	number	NOUN
ajst-25631	28	36	of	of	ADP
ajst-25631	28	37	recombinant	recombinant	ADJ
ajst-25631	28	38	proteins	protein	NOUN
ajst-25631	28	39	[	[	X
ajst-25631	28	40	9	9	NUM
ajst-25631	28	41	]	]	PUNCT
ajst-25631	28	42	.	.	PUNCT
ajst-25631	29	1	the	the	DET
ajst-25631	29	2	medium	medium	NOUN
ajst-25631	29	3	required	require	VERB
ajst-25631	29	4	for	for	ADP
ajst-25631	29	5	e.	e.	PROPN
ajst-25631	29	6	coli	coli	PROPN
ajst-25631	29	7	is	be	AUX
ajst-25631	29	8	inexpensive	inexpensive	ADJ
ajst-25631	29	9	and	and	CCONJ
ajst-25631	29	10	easy	easy	ADJ
ajst-25631	29	11	to	to	PART
ajst-25631	29	12	prepare	prepare	VERB
ajst-25631	29	13	,	,	PUNCT
ajst-25631	29	14	and	and	CCONJ
ajst-25631	29	15	the	the	DET
ajst-25631	29	16	culture	culture	NOUN
ajst-25631	29	17	process	process	NOUN
ajst-25631	29	18	is	be	AUX
ajst-25631	29	19	often	often	ADV
ajst-25631	29	20	simpler	simple	ADJ
ajst-25631	29	21	and	and	CCONJ
ajst-25631	29	22	more	more	ADV
ajst-25631	29	23	convenient	convenient	ADJ
ajst-25631	29	24	than	than	ADP
ajst-25631	29	25	other	other	ADJ
ajst-25631	29	26	systems	system	NOUN
ajst-25631	29	27	[	[	X
ajst-25631	29	28	7,10	7,10	X
ajst-25631	29	29	]	]	PUNCT
ajst-25631	29	30	.	.	PUNCT
ajst-25631	30	1	e.	e.	PROPN
ajst-25631	30	2	coli	coli	PROPN
ajst-25631	30	3	can	can	AUX
ajst-25631	30	4	adapt	adapt	VERB
ajst-25631	30	5	to	to	ADP
ajst-25631	30	6	a	a	DET
ajst-25631	30	7	variety	variety	NOUN
ajst-25631	30	8	of	of	ADP
ajst-25631	30	9	growth	growth	NOUN
ajst-25631	30	10	conditions	condition	NOUN
ajst-25631	30	11	,	,	PUNCT
ajst-25631	30	12	including	include	VERB
ajst-25631	30	13	different	different	ADJ
ajst-25631	30	14	temperatures	temperature	NOUN
ajst-25631	30	15	,	,	PUNCT
ajst-25631	30	16	ph	ph	NOUN
ajst-25631	30	17	,	,	PUNCT
ajst-25631	30	18	and	and	CCONJ
ajst-25631	30	19	oxygen	oxygen	NOUN
ajst-25631	30	20	concentrations	concentration	NOUN
ajst-25631	30	21	.	.	PUNCT
ajst-25631	31	1	this	this	DET
ajst-25631	31	2	adaptability	adaptability	NOUN
ajst-25631	31	3	enables	enable	VERB
ajst-25631	31	4	efficient	efficient	ADJ
ajst-25631	31	5	production	production	NOUN
ajst-25631	31	6	of	of	ADP
ajst-25631	31	7	target	target	NOUN
ajst-25631	31	8	proteins	protein	NOUN
ajst-25631	31	9	under	under	ADP
ajst-25631	31	10	different	different	ADJ
ajst-25631	31	11	production	production	NOUN
ajst-25631	31	12	environments	environment	NOUN
ajst-25631	31	13	.	.	PUNCT
ajst-25631	32	1	in	in	ADP
ajst-25631	32	2	addition	addition	NOUN
ajst-25631	32	3	,	,	PUNCT
ajst-25631	32	4	by	by	ADP
ajst-25631	32	5	means	mean	NOUN
ajst-25631	32	6	of	of	ADP
ajst-25631	32	7	genetic	genetic	ADJ
ajst-25631	32	8	engineering	engineering	NOUN
ajst-25631	32	9	,	,	PUNCT
ajst-25631	32	10	its	its	PRON
ajst-25631	32	11	metabolic	metabolic	NOUN
ajst-25631	32	12	pathway	pathway	NOUN
ajst-25631	32	13	can	can	AUX
ajst-25631	32	14	be	be	AUX
ajst-25631	32	15	further	far	ADV
ajst-25631	32	16	optimized	optimize	VERB
ajst-25631	32	17	to	to	PART
ajst-25631	32	18	enhance	enhance	VERB
ajst-25631	32	19	the	the	DET
ajst-25631	32	20	yield	yield	NOUN
ajst-25631	32	21	and	and	CCONJ
ajst-25631	32	22	quality	quality	NOUN
ajst-25631	32	23	of	of	ADP
ajst-25631	32	24	recombinant	recombinant	ADJ
ajst-25631	32	25	proteins	protein	NOUN
ajst-25631	32	26	[	[	X
ajst-25631	32	27	6,9	6,9	NUM
ajst-25631	32	28	]	]	PUNCT
ajst-25631	32	29	.	.	PUNCT
ajst-25631	33	1	224	224	NUM
ajst-25631	33	2	these	these	DET
ajst-25631	33	3	properties	property	NOUN
ajst-25631	33	4	make	make	VERB
ajst-25631	33	5	it	it	PRON
ajst-25631	33	6	an	an	DET
ajst-25631	33	7	important	important	ADJ
ajst-25631	33	8	tool	tool	NOUN
ajst-25631	33	9	in	in	ADP
ajst-25631	33	10	the	the	DET
ajst-25631	33	11	field	field	NOUN
ajst-25631	33	12	of	of	ADP
ajst-25631	33	13	biotechnology	biotechnology	NOUN
ajst-25631	33	14	and	and	CCONJ
ajst-25631	33	15	also	also	ADV
ajst-25631	33	16	make	make	VERB
ajst-25631	33	17	the	the	DET
ajst-25631	33	18	use	use	NOUN
ajst-25631	33	19	of	of	ADP
ajst-25631	33	20	e.	e.	PROPN
ajst-25631	33	21	coli	coli	PROPN
ajst-25631	33	22	in	in	ADP
ajst-25631	33	23	industrial	industrial	ADJ
ajst-25631	33	24	production	production	NOUN
ajst-25631	33	25	a	a	DET
ajst-25631	33	26	cost	cost	NOUN
ajst-25631	33	27	-	-	PUNCT
ajst-25631	33	28	effective	effective	ADJ
ajst-25631	33	29	option	option	NOUN
ajst-25631	33	30	.	.	PUNCT
ajst-25631	34	1	3	3	X
ajst-25631	34	2	.	.	X
ajst-25631	34	3	challenges	challenge	NOUN
ajst-25631	34	4	of	of	ADP
ajst-25631	34	5	escherichia	escherichia	NOUN
ajst-25631	34	6	coli	coli	NOUN
ajst-25631	34	7	as	as	ADP
ajst-25631	34	8	an	an	DET
ajst-25631	34	9	expression	expression	NOUN
ajst-25631	34	10	host	host	NOUN
ajst-25631	34	11	despite	despite	SCONJ
ajst-25631	34	12	e.	e.	PROPN
ajst-25631	34	13	coli	coli	PROPN
ajst-25631	34	14	's	's	PART
ajst-25631	34	15	strengths	strength	NOUN
ajst-25631	34	16	in	in	ADP
ajst-25631	34	17	genetic	genetic	ADJ
ajst-25631	34	18	engineering	engineering	NOUN
ajst-25631	34	19	and	and	CCONJ
ajst-25631	34	20	protein	protein	NOUN
ajst-25631	34	21	expression	expression	NOUN
ajst-25631	34	22	,	,	PUNCT
ajst-25631	34	23	it	it	PRON
ajst-25631	34	24	still	still	ADV
ajst-25631	34	25	faces	face	VERB
ajst-25631	34	26	many	many	ADJ
ajst-25631	34	27	challenges	challenge	NOUN
ajst-25631	34	28	in	in	ADP
ajst-25631	34	29	the	the	DET
ajst-25631	34	30	folding	folding	NOUN
ajst-25631	34	31	and	and	CCONJ
ajst-25631	34	32	aggregation	aggregation	NOUN
ajst-25631	34	33	of	of	ADP
ajst-25631	34	34	recombinant	recombinant	ADJ
ajst-25631	34	35	proteins	protein	NOUN
ajst-25631	34	36	.	.	PUNCT
ajst-25631	35	1	when	when	SCONJ
ajst-25631	35	2	e.	e.	PROPN
ajst-25631	35	3	coli	coli	PROPN
ajst-25631	35	4	expresses	express	VERB
ajst-25631	35	5	recombinant	recombinant	ADJ
ajst-25631	35	6	protein	protein	NOUN
ajst-25631	35	7	at	at	ADP
ajst-25631	35	8	high	high	ADJ
ajst-25631	35	9	levels	level	NOUN
ajst-25631	35	10	,	,	PUNCT
ajst-25631	35	11	it	it	PRON
ajst-25631	35	12	is	be	AUX
ajst-25631	35	13	easy	easy	ADJ
ajst-25631	35	14	to	to	PART
ajst-25631	35	15	cause	cause	VERB
ajst-25631	35	16	the	the	DET
ajst-25631	35	17	protein	protein	NOUN
ajst-25631	35	18	to	to	PART
ajst-25631	35	19	aggregate	aggregate	VERB
ajst-25631	35	20	into	into	ADP
ajst-25631	35	21	inclusion	inclusion	NOUN
ajst-25631	35	22	bodies	body	NOUN
ajst-25631	35	23	.	.	PUNCT
ajst-25631	36	1	this	this	DET
ajst-25631	36	2	phenomenon	phenomenon	NOUN
ajst-25631	36	3	is	be	AUX
ajst-25631	36	4	usually	usually	ADV
ajst-25631	36	5	caused	cause	VERB
ajst-25631	36	6	by	by	ADP
ajst-25631	36	7	factors	factor	NOUN
ajst-25631	36	8	such	such	ADJ
ajst-25631	36	9	as	as	ADP
ajst-25631	36	10	high	high	ADJ
ajst-25631	36	11	temperature	temperature	NOUN
ajst-25631	36	12	,	,	PUNCT
ajst-25631	36	13	high	high	ADJ
ajst-25631	36	14	induced	induced	ADJ
ajst-25631	36	15	concentration	concentration	NOUN
ajst-25631	36	16	and	and	CCONJ
ajst-25631	36	17	strong	strong	ADJ
ajst-25631	36	18	start	start	VERB
ajst-25631	36	19	-	-	PUNCT
ajst-25631	36	20	up	up	ADP
ajst-25631	36	21	subsystem	subsystem	NOUN
ajst-25631	36	22	[	[	X
ajst-25631	36	23	11,12	11,12	NOUN
ajst-25631	36	24	]	]	PUNCT
ajst-25631	36	25	.	.	PUNCT
ajst-25631	37	1	under	under	ADP
ajst-25631	37	2	these	these	DET
ajst-25631	37	3	conditions	condition	NOUN
ajst-25631	37	4	,	,	PUNCT
ajst-25631	37	5	the	the	DET
ajst-25631	37	6	translation	translation	NOUN
ajst-25631	37	7	rate	rate	NOUN
ajst-25631	37	8	of	of	ADP
ajst-25631	37	9	the	the	DET
ajst-25631	37	10	protein	protein	NOUN
ajst-25631	37	11	increases	increase	NOUN
ajst-25631	37	12	,	,	PUNCT
ajst-25631	37	13	resulting	result	VERB
ajst-25631	37	14	in	in	ADP
ajst-25631	37	15	insufficient	insufficient	ADJ
ajst-25631	37	16	folding	folding	NOUN
ajst-25631	37	17	time	time	NOUN
ajst-25631	37	18	,	,	PUNCT
ajst-25631	37	19	resulting	result	VERB
ajst-25631	37	20	in	in	ADP
ajst-25631	37	21	the	the	DET
ajst-25631	37	22	formation	formation	NOUN
ajst-25631	37	23	of	of	ADP
ajst-25631	37	24	nonfunctional	nonfunctional	ADJ
ajst-25631	37	25	aggregates	aggregate	NOUN
ajst-25631	37	26	.	.	PUNCT
ajst-25631	38	1	some	some	DET
ajst-25631	38	2	recombinant	recombinant	ADJ
ajst-25631	38	3	proteins	protein	NOUN
ajst-25631	38	4	have	have	VERB
ajst-25631	38	5	complex	complex	ADJ
ajst-25631	38	6	structures	structure	NOUN
ajst-25631	38	7	or	or	CCONJ
ajst-25631	38	8	are	be	AUX
ajst-25631	38	9	highly	highly	ADV
ajst-25631	38	10	toxic	toxic	ADJ
ajst-25631	38	11	,	,	PUNCT
ajst-25631	38	12	and	and	CCONJ
ajst-25631	38	13	their	their	PRON
ajst-25631	38	14	intracellular	intracellular	ADJ
ajst-25631	38	15	expression	expression	NOUN
ajst-25631	38	16	may	may	AUX
ajst-25631	38	17	burden	burden	VERB
ajst-25631	38	18	host	host	NOUN
ajst-25631	38	19	cells	cell	NOUN
ajst-25631	38	20	and	and	CCONJ
ajst-25631	38	21	increase	increase	VERB
ajst-25631	38	22	the	the	DET
ajst-25631	38	23	risk	risk	NOUN
ajst-25631	38	24	of	of	ADP
ajst-25631	38	25	misfolding	misfolde	VERB
ajst-25631	38	26	[	[	X
ajst-25631	38	27	6	6	NUM
ajst-25631	38	28	]	]	PUNCT
ajst-25631	38	29	.	.	PUNCT
ajst-25631	39	1	in	in	ADP
ajst-25631	39	2	addition	addition	NOUN
ajst-25631	39	3	,	,	PUNCT
ajst-25631	39	4	many	many	ADJ
ajst-25631	39	5	heteropeptides	heteropeptide	NOUN
ajst-25631	39	6	,	,	PUNCT
ajst-25631	39	7	when	when	SCONJ
ajst-25631	39	8	expressed	express	VERB
ajst-25631	39	9	in	in	ADP
ajst-25631	39	10	e.	e.	PROPN
ajst-25631	39	11	coli	coli	PROPN
ajst-25631	39	12	,	,	PUNCT
ajst-25631	39	13	fail	fail	VERB
ajst-25631	39	14	to	to	PART
ajst-25631	39	15	fold	fold	VERB
ajst-25631	39	16	into	into	ADP
ajst-25631	39	17	their	their	PRON
ajst-25631	39	18	natural	natural	ADJ
ajst-25631	39	19	state	state	NOUN
ajst-25631	39	20	and	and	CCONJ
ajst-25631	39	21	are	be	AUX
ajst-25631	39	22	subsequently	subsequently	ADV
ajst-25631	39	23	degraded	degrade	VERB
ajst-25631	39	24	by	by	ADP
ajst-25631	39	25	cells	cell	NOUN
ajst-25631	39	26	or	or	CCONJ
ajst-25631	39	27	aggregated	aggregate	VERB
ajst-25631	39	28	into	into	ADP
ajst-25631	39	29	insoluble	insoluble	ADJ
ajst-25631	39	30	forms	form	NOUN
ajst-25631	39	31	[	[	X
ajst-25631	39	32	13,14	13,14	NUM
ajst-25631	39	33	]	]	PUNCT
ajst-25631	39	34	.	.	PUNCT
ajst-25631	40	1	changes	change	NOUN
ajst-25631	40	2	in	in	ADP
ajst-25631	40	3	the	the	DET
ajst-25631	40	4	intracellular	intracellular	ADJ
ajst-25631	40	5	environment	environment	NOUN
ajst-25631	40	6	,	,	PUNCT
ajst-25631	40	7	such	such	ADJ
ajst-25631	40	8	as	as	ADP
ajst-25631	40	9	ph	ph	ADJ
ajst-25631	40	10	,	,	PUNCT
ajst-25631	40	11	salt	salt	NOUN
ajst-25631	40	12	concentration	concentration	NOUN
ajst-25631	40	13	and	and	CCONJ
ajst-25631	40	14	temperature	temperature	NOUN
ajst-25631	40	15	,	,	PUNCT
ajst-25631	40	16	also	also	ADV
ajst-25631	40	17	affect	affect	VERB
ajst-25631	40	18	the	the	DET
ajst-25631	40	19	folding	fold	VERB
ajst-25631	40	20	process	process	NOUN
ajst-25631	40	21	of	of	ADP
ajst-25631	40	22	proteins	protein	NOUN
ajst-25631	40	23	[	[	X
ajst-25631	40	24	15,16	15,16	NUM
ajst-25631	40	25	]	]	PUNCT
ajst-25631	40	26	.	.	PUNCT
ajst-25631	41	1	4	4	X
ajst-25631	41	2	.	.	X
ajst-25631	41	3	strategies	strategy	NOUN
ajst-25631	41	4	for	for	ADP
ajst-25631	41	5	optimization	optimization	NOUN
ajst-25631	41	6	4.1	4.1	NUM
ajst-25631	41	7	.	.	PUNCT
ajst-25631	42	1	optimization	optimization	NOUN
ajst-25631	42	2	of	of	ADP
ajst-25631	42	3	clone	clone	NOUN
ajst-25631	42	4	design	design	NOUN
ajst-25631	42	5	commonly	commonly	ADV
ajst-25631	42	6	used	use	VERB
ajst-25631	42	7	e.	e.	PROPN
ajst-25631	42	8	coli	coli	NOUN
ajst-25631	42	9	carriers	carrier	NOUN
ajst-25631	42	10	are	be	AUX
ajst-25631	42	11	pet	pet	ADJ
ajst-25631	42	12	,	,	PUNCT
ajst-25631	42	13	pcold	pcold	ADJ
ajst-25631	42	14	,	,	PUNCT
ajst-25631	42	15	pbv	pbv	VERB
ajst-25631	42	16	and	and	CCONJ
ajst-25631	42	17	pgex	pgex	VERB
ajst-25631	42	18	series	series	NOUN
ajst-25631	42	19	.	.	PUNCT
ajst-25631	43	1	the	the	DET
ajst-25631	43	2	component	component	NOUN
ajst-25631	43	3	composition	composition	NOUN
ajst-25631	43	4	and	and	CCONJ
ajst-25631	43	5	promoter	promoter	NOUN
ajst-25631	43	6	type	type	NOUN
ajst-25631	43	7	of	of	ADP
ajst-25631	43	8	the	the	DET
ajst-25631	43	9	vector	vector	NOUN
ajst-25631	43	10	affect	affect	VERB
ajst-25631	43	11	the	the	DET
ajst-25631	43	12	gene	gene	NOUN
ajst-25631	43	13	expression	expression	NOUN
ajst-25631	43	14	level	level	NOUN
ajst-25631	43	15	and	and	CCONJ
ajst-25631	43	16	solubility	solubility	NOUN
ajst-25631	43	17	.	.	PUNCT
ajst-25631	44	1	it	it	PRON
ajst-25631	44	2	is	be	AUX
ajst-25631	44	3	very	very	ADV
ajst-25631	44	4	important	important	ADJ
ajst-25631	44	5	to	to	PART
ajst-25631	44	6	select	select	VERB
ajst-25631	44	7	suitable	suitable	ADJ
ajst-25631	44	8	expression	expression	NOUN
ajst-25631	44	9	vector	vector	NOUN
ajst-25631	44	10	to	to	PART
ajst-25631	44	11	improve	improve	VERB
ajst-25631	44	12	protein	protein	NOUN
ajst-25631	44	13	expression	expression	NOUN
ajst-25631	44	14	.	.	PUNCT
ajst-25631	45	1	differences	difference	NOUN
ajst-25631	45	2	in	in	ADP
ajst-25631	45	3	codon	codon	ADJ
ajst-25631	45	4	use	use	NOUN
ajst-25631	45	5	between	between	ADP
ajst-25631	45	6	e.	e.	PROPN
ajst-25631	45	7	coli	coli	NOUN
ajst-25631	45	8	and	and	CCONJ
ajst-25631	45	9	eukaryotes	eukaryote	NOUN
ajst-25631	45	10	may	may	AUX
ajst-25631	45	11	result	result	VERB
ajst-25631	45	12	in	in	ADP
ajst-25631	45	13	inadequate	inadequate	ADJ
ajst-25631	45	14	expression	expression	NOUN
ajst-25631	45	15	of	of	ADP
ajst-25631	45	16	foreign	foreign	ADJ
ajst-25631	45	17	genes	gene	NOUN
ajst-25631	45	18	.	.	PUNCT
ajst-25631	46	1	therefore	therefore	ADV
ajst-25631	46	2	,	,	PUNCT
ajst-25631	46	3	codon	codon	ADJ
ajst-25631	46	4	optimization	optimization	NOUN
ajst-25631	46	5	is	be	AUX
ajst-25631	46	6	an	an	DET
ajst-25631	46	7	effective	effective	ADJ
ajst-25631	46	8	method	method	NOUN
ajst-25631	46	9	to	to	PART
ajst-25631	46	10	improve	improve	VERB
ajst-25631	46	11	the	the	DET
ajst-25631	46	12	expression	expression	NOUN
ajst-25631	46	13	of	of	ADP
ajst-25631	46	14	recombinant	recombinant	ADJ
ajst-25631	46	15	protein	protein	NOUN
ajst-25631	46	16	.	.	PUNCT
ajst-25631	47	1	by	by	ADP
ajst-25631	47	2	adjusting	adjust	VERB
ajst-25631	47	3	the	the	DET
ajst-25631	47	4	codon	codon	NOUN
ajst-25631	47	5	of	of	ADP
ajst-25631	47	6	the	the	DET
ajst-25631	47	7	target	target	NOUN
ajst-25631	47	8	gene	gene	NOUN
ajst-25631	47	9	to	to	PART
ajst-25631	47	10	better	well	ADV
ajst-25631	47	11	match	match	VERB
ajst-25631	47	12	the	the	DET
ajst-25631	47	13	preference	preference	NOUN
ajst-25631	47	14	codon	codon	NOUN
ajst-25631	47	15	of	of	ADP
ajst-25631	47	16	escherichia	escherichia	NOUN
ajst-25631	47	17	coli	coli	NOUN
ajst-25631	47	18	,	,	PUNCT
ajst-25631	47	19	the	the	DET
ajst-25631	47	20	expression	expression	NOUN
ajst-25631	47	21	level	level	NOUN
ajst-25631	47	22	of	of	ADP
ajst-25631	47	23	the	the	DET
ajst-25631	47	24	protein	protein	NOUN
ajst-25631	47	25	can	can	AUX
ajst-25631	47	26	be	be	AUX
ajst-25631	47	27	significantly	significantly	ADV
ajst-25631	47	28	improved	improve	VERB
ajst-25631	47	29	[	[	X
ajst-25631	47	30	17	17	NUM
ajst-25631	47	31	]	]	PUNCT
ajst-25631	47	32	.	.	PUNCT
ajst-25631	48	1	it	it	PRON
ajst-25631	48	2	has	have	AUX
ajst-25631	48	3	been	be	AUX
ajst-25631	48	4	found	find	VERB
ajst-25631	48	5	that	that	SCONJ
ajst-25631	48	6	replacing	replace	VERB
ajst-25631	48	7	the	the	DET
ajst-25631	48	8	rare	rare	ADJ
ajst-25631	48	9	arginine	arginine	NOUN
ajst-25631	48	10	codon	codon	VERB
ajst-25631	48	11	with	with	ADP
ajst-25631	48	12	the	the	DET
ajst-25631	48	13	more	more	ADV
ajst-25631	48	14	commonly	commonly	ADV
ajst-25631	48	15	used	use	VERB
ajst-25631	48	16	codon	codon	NOUN
ajst-25631	48	17	increases	increase	VERB
ajst-25631	48	18	the	the	DET
ajst-25631	48	19	production	production	NOUN
ajst-25631	48	20	of	of	ADP
ajst-25631	48	21	human	human	ADJ
ajst-25631	48	22	interferon	interferon	PROPN
ajst-25631	48	23	2b	2b	NUM
ajst-25631	48	24	in	in	ADP
ajst-25631	48	25	e.	e.	PROPN
ajst-25631	48	26	coli	coli	NOUN
ajst-25631	48	27	by	by	ADP
ajst-25631	48	28	about	about	ADV
ajst-25631	48	29	10	10	NUM
ajst-25631	48	30	times	time	NOUN
ajst-25631	48	31	[	[	X
ajst-25631	48	32	18	18	NUM
ajst-25631	48	33	]	]	PUNCT
ajst-25631	48	34	.	.	PUNCT
ajst-25631	49	1	fusion	fusion	NOUN
ajst-25631	49	2	tags	tag	NOUN
ajst-25631	49	3	are	be	AUX
ajst-25631	49	4	often	often	ADV
ajst-25631	49	5	used	use	VERB
ajst-25631	49	6	to	to	PART
ajst-25631	49	7	improve	improve	VERB
ajst-25631	49	8	protein	protein	NOUN
ajst-25631	49	9	solubility	solubility	NOUN
ajst-25631	49	10	and	and	CCONJ
ajst-25631	49	11	purification	purification	NOUN
ajst-25631	49	12	processes	process	NOUN
ajst-25631	49	13	.	.	PUNCT
ajst-25631	50	1	in	in	ADP
ajst-25631	50	2	clonal	clonal	ADJ
ajst-25631	50	3	design	design	NOUN
ajst-25631	50	4	,	,	PUNCT
ajst-25631	50	5	some	some	DET
ajst-25631	50	6	tags	tag	NOUN
ajst-25631	50	7	are	be	AUX
ajst-25631	50	8	often	often	ADV
ajst-25631	50	9	fused	fuse	VERB
ajst-25631	50	10	to	to	ADP
ajst-25631	50	11	the	the	DET
ajst-25631	50	12	n	n	CCONJ
ajst-25631	50	13	-	-	PUNCT
ajst-25631	50	14	terminal	terminal	ADJ
ajst-25631	50	15	or	or	CCONJ
ajst-25631	50	16	c	c	NOUN
ajst-25631	50	17	-	-	PUNCT
ajst-25631	50	18	terminal	terminal	NOUN
ajst-25631	50	19	of	of	ADP
ajst-25631	50	20	the	the	DET
ajst-25631	50	21	target	target	NOUN
ajst-25631	50	22	protein	protein	NOUN
ajst-25631	50	23	,	,	PUNCT
ajst-25631	50	24	such	such	ADJ
ajst-25631	50	25	as	as	ADP
ajst-25631	50	26	his	his	PRON
ajst-25631	50	27	,	,	PUNCT
ajst-25631	50	28	gst	gst	PROPN
ajst-25631	50	29	,	,	PUNCT
ajst-25631	50	30	mbp	mbp	NOUN
ajst-25631	50	31	,	,	PUNCT
ajst-25631	50	32	nusa	nusa	PROPN
ajst-25631	50	33	,	,	PUNCT
ajst-25631	50	34	trx	trx	PROPN
ajst-25631	50	35	,	,	PUNCT
ajst-25631	50	36	and	and	CCONJ
ajst-25631	50	37	sumo	sumo	NOUN
ajst-25631	50	38	.	.	PUNCT
ajst-25631	51	1	however	however	ADV
ajst-25631	51	2	,	,	PUNCT
ajst-25631	51	3	the	the	DET
ajst-25631	51	4	main	main	ADJ
ajst-25631	51	5	challenge	challenge	NOUN
ajst-25631	51	6	in	in	ADP
ajst-25631	51	7	protein	protein	NOUN
ajst-25631	51	8	production	production	NOUN
ajst-25631	51	9	is	be	AUX
ajst-25631	51	10	how	how	SCONJ
ajst-25631	51	11	to	to	PART
ajst-25631	51	12	split	split	VERB
ajst-25631	51	13	and	and	CCONJ
ajst-25631	51	14	remove	remove	VERB
ajst-25631	51	15	fusion	fusion	NOUN
ajst-25631	51	16	tags	tag	NOUN
ajst-25631	51	17	during	during	ADP
ajst-25631	51	18	the	the	DET
ajst-25631	51	19	different	different	ADJ
ajst-25631	51	20	steps	step	NOUN
ajst-25631	51	21	of	of	ADP
ajst-25631	51	22	purification	purification	NOUN
ajst-25631	51	23	.	.	PUNCT
ajst-25631	52	1	to	to	PART
ajst-25631	52	2	achieve	achieve	VERB
ajst-25631	52	3	this	this	PRON
ajst-25631	52	4	,	,	PUNCT
ajst-25631	52	5	multiple	multiple	ADJ
ajst-25631	52	6	cleavage	cleavage	NOUN
ajst-25631	52	7	sites	site	NOUN
ajst-25631	52	8	have	have	AUX
ajst-25631	52	9	been	be	AUX
ajst-25631	52	10	designed	design	VERB
ajst-25631	52	11	for	for	ADP
ajst-25631	52	12	proper	proper	ADJ
ajst-25631	52	13	protein	protein	NOUN
ajst-25631	52	14	expression	expression	NOUN
ajst-25631	52	15	and	and	CCONJ
ajst-25631	52	16	cleavage	cleavage	NOUN
ajst-25631	52	17	.	.	PUNCT
ajst-25631	53	1	in	in	ADP
ajst-25631	53	2	addition	addition	NOUN
ajst-25631	53	3	,	,	PUNCT
ajst-25631	53	4	in	in	ADP
ajst-25631	53	5	some	some	DET
ajst-25631	53	6	cases	case	NOUN
ajst-25631	53	7	,	,	PUNCT
ajst-25631	53	8	the	the	DET
ajst-25631	53	9	target	target	NOUN
ajst-25631	53	10	protein	protein	NOUN
ajst-25631	53	11	may	may	AUX
ajst-25631	53	12	result	result	VERB
ajst-25631	53	13	in	in	ADP
ajst-25631	53	14	low	low	ADJ
ajst-25631	53	15	expression	expression	NOUN
ajst-25631	53	16	due	due	ADP
ajst-25631	53	17	to	to	ADP
ajst-25631	53	18	misfolding	misfolding	NOUN
ajst-25631	53	19	or	or	CCONJ
ajst-25631	53	20	aggregation	aggregation	NOUN
ajst-25631	53	21	,	,	PUNCT
ajst-25631	53	22	in	in	ADP
ajst-25631	53	23	which	which	DET
ajst-25631	53	24	case	case	NOUN
ajst-25631	53	25	,	,	PUNCT
ajst-25631	53	26	coexpression	coexpression	NOUN
ajst-25631	53	27	of	of	ADP
ajst-25631	53	28	molecular	molecular	ADJ
ajst-25631	53	29	chaperones	chaperone	NOUN
ajst-25631	53	30	(	(	PUNCT
ajst-25631	53	31	such	such	ADJ
ajst-25631	53	32	as	as	ADP
ajst-25631	53	33	dnak	dnak	NOUN
ajst-25631	53	34	,	,	PUNCT
ajst-25631	53	35	groel	groel	NOUN
ajst-25631	53	36	,	,	PUNCT
ajst-25631	53	37	groes	groe	NOUN
ajst-25631	53	38	,	,	PUNCT
ajst-25631	53	39	etc	etc	X
ajst-25631	53	40	.	.	X
ajst-25631	53	41	)	)	PUNCT
ajst-25631	53	42	can	can	AUX
ajst-25631	53	43	be	be	AUX
ajst-25631	53	44	considered	consider	VERB
ajst-25631	53	45	to	to	PART
ajst-25631	53	46	promote	promote	VERB
ajst-25631	53	47	the	the	DET
ajst-25631	53	48	correct	correct	ADJ
ajst-25631	53	49	folding	folding	NOUN
ajst-25631	53	50	of	of	ADP
ajst-25631	53	51	the	the	DET
ajst-25631	53	52	target	target	NOUN
ajst-25631	53	53	protein	protein	NOUN
ajst-25631	53	54	,	,	PUNCT
ajst-25631	53	55	improve	improve	VERB
ajst-25631	53	56	solubility	solubility	NOUN
ajst-25631	53	57	and	and	CCONJ
ajst-25631	53	58	reduce	reduce	VERB
ajst-25631	53	59	the	the	DET
ajst-25631	53	60	formation	formation	NOUN
ajst-25631	53	61	of	of	ADP
ajst-25631	53	62	inclusion	inclusion	NOUN
ajst-25631	53	63	bodies	body	NOUN
ajst-25631	53	64	.	.	PUNCT
ajst-25631	54	1	this	this	DET
ajst-25631	54	2	strategy	strategy	NOUN
ajst-25631	54	3	has	have	AUX
ajst-25631	54	4	been	be	AUX
ajst-25631	54	5	shown	show	VERB
ajst-25631	54	6	to	to	PART
ajst-25631	54	7	increase	increase	VERB
ajst-25631	54	8	the	the	DET
ajst-25631	54	9	production	production	NOUN
ajst-25631	54	10	and	and	CCONJ
ajst-25631	54	11	solubility	solubility	NOUN
ajst-25631	54	12	of	of	ADP
ajst-25631	54	13	difficult	difficult	ADJ
ajst-25631	54	14	-	-	PUNCT
ajst-25631	54	15	toexpress	toexpress	ADJ
ajst-25631	54	16	proteins	protein	NOUN
ajst-25631	54	17	[	[	X
ajst-25631	54	18	6,19,20	6,19,20	NUM
ajst-25631	54	19	]	]	X
ajst-25631	54	20	.	.	PUNCT
ajst-25631	55	1	4.2	4.2	NUM
ajst-25631	55	2	.	.	PUNCT
ajst-25631	55	3	optimization	optimization	NOUN
ajst-25631	55	4	of	of	ADP
ajst-25631	55	5	culture	culture	NOUN
ajst-25631	55	6	and	and	CCONJ
ajst-25631	55	7	induction	induction	NOUN
ajst-25631	55	8	optimizing	optimize	VERB
ajst-25631	55	9	induction	induction	NOUN
ajst-25631	55	10	conditions	condition	NOUN
ajst-25631	55	11	in	in	ADP
ajst-25631	55	12	escherichia	escherichia	NOUN
ajst-25631	55	13	coli	coli	NOUN
ajst-25631	55	14	is	be	AUX
ajst-25631	55	15	a	a	DET
ajst-25631	55	16	complex	complex	ADJ
ajst-25631	55	17	and	and	CCONJ
ajst-25631	55	18	important	important	ADJ
ajst-25631	55	19	process	process	NOUN
ajst-25631	55	20	involving	involve	VERB
ajst-25631	55	21	the	the	DET
ajst-25631	55	22	comprehensive	comprehensive	ADJ
ajst-25631	55	23	consideration	consideration	NOUN
ajst-25631	55	24	of	of	ADP
ajst-25631	55	25	several	several	ADJ
ajst-25631	55	26	factors	factor	NOUN
ajst-25631	55	27	.	.	PUNCT
ajst-25631	56	1	the	the	DET
ajst-25631	56	2	expression	expression	NOUN
ajst-25631	56	3	efficiency	efficiency	NOUN
ajst-25631	56	4	of	of	ADP
ajst-25631	56	5	recombinant	recombinant	ADJ
ajst-25631	56	6	protein	protein	NOUN
ajst-25631	56	7	could	could	AUX
ajst-25631	56	8	be	be	AUX
ajst-25631	56	9	significantly	significantly	ADV
ajst-25631	56	10	improved	improve	VERB
ajst-25631	56	11	by	by	ADP
ajst-25631	56	12	selecting	select	VERB
ajst-25631	56	13	the	the	DET
ajst-25631	56	14	inducer	inducer	NOUN
ajst-25631	56	15	and	and	CCONJ
ajst-25631	56	16	its	its	PRON
ajst-25631	56	17	concentration	concentration	NOUN
ajst-25631	56	18	,	,	PUNCT
ajst-25631	56	19	induction	induction	NOUN
ajst-25631	56	20	time	time	NOUN
ajst-25631	56	21	,	,	PUNCT
ajst-25631	56	22	culture	culture	NOUN
ajst-25631	56	23	temperature	temperature	NOUN
ajst-25631	56	24	and	and	CCONJ
ajst-25631	56	25	medium	medium	NOUN
ajst-25631	56	26	composition	composition	NOUN
ajst-25631	56	27	.	.	PUNCT
ajst-25631	57	1	the	the	DET
ajst-25631	57	2	type	type	NOUN
ajst-25631	57	3	and	and	CCONJ
ajst-25631	57	4	concentration	concentration	NOUN
ajst-25631	57	5	of	of	ADP
ajst-25631	57	6	the	the	DET
ajst-25631	57	7	inducer	inducer	NOUN
ajst-25631	57	8	have	have	VERB
ajst-25631	57	9	significant	significant	ADJ
ajst-25631	57	10	influence	influence	NOUN
ajst-25631	57	11	on	on	ADP
ajst-25631	57	12	protein	protein	NOUN
ajst-25631	57	13	expression	expression	NOUN
ajst-25631	57	14	,	,	PUNCT
ajst-25631	57	15	and	and	CCONJ
ajst-25631	57	16	it	it	PRON
ajst-25631	57	17	is	be	AUX
ajst-25631	57	18	very	very	ADV
ajst-25631	57	19	important	important	ADJ
ajst-25631	57	20	to	to	PART
ajst-25631	57	21	select	select	VERB
ajst-25631	57	22	the	the	DET
ajst-25631	57	23	appropriate	appropriate	ADJ
ajst-25631	57	24	inducer	inducer	NOUN
ajst-25631	57	25	and	and	CCONJ
ajst-25631	57	26	its	its	PRON
ajst-25631	57	27	concentration	concentration	NOUN
ajst-25631	57	28	to	to	PART
ajst-25631	57	29	obtain	obtain	VERB
ajst-25631	57	30	high	high	ADJ
ajst-25631	57	31	yield	yield	NOUN
ajst-25631	57	32	of	of	ADP
ajst-25631	57	33	recombinant	recombinant	ADJ
ajst-25631	57	34	protein	protein	NOUN
ajst-25631	57	35	.	.	PUNCT
ajst-25631	58	1	the	the	DET
ajst-25631	58	2	most	most	ADV
ajst-25631	58	3	commonly	commonly	ADV
ajst-25631	58	4	used	use	VERB
ajst-25631	58	5	inducer	inducer	NOUN
ajst-25631	58	6	is	be	AUX
ajst-25631	58	7	isopropyl	isopropyl	NOUN
ajst-25631	58	8	-	-	PUNCT
ajst-25631	58	9	beta	beta	NOUN
ajst-25631	58	10	-	-	PUNCT
ajst-25631	58	11	d	d	NOUN
ajst-25631	58	12	-	-	PUNCT
ajst-25631	58	13	thiogalactoside	thiogalactoside	NOUN
ajst-25631	58	14	(	(	PUNCT
ajst-25631	58	15	iptg	iptg	NOUN
ajst-25631	58	16	)	)	PUNCT
ajst-25631	58	17	.	.	PUNCT
ajst-25631	59	1	in	in	ADP
ajst-25631	59	2	general	general	ADJ
ajst-25631	59	3	,	,	PUNCT
ajst-25631	59	4	excessively	excessively	ADV
ajst-25631	59	5	high	high	ADJ
ajst-25631	59	6	concentrations	concentration	NOUN
ajst-25631	59	7	of	of	ADP
ajst-25631	59	8	iptg	iptg	NOUN
ajst-25631	59	9	may	may	AUX
ajst-25631	59	10	lead	lead	VERB
ajst-25631	59	11	to	to	ADP
ajst-25631	59	12	the	the	DET
ajst-25631	59	13	accumulation	accumulation	NOUN
ajst-25631	59	14	of	of	ADP
ajst-25631	59	15	protein	protein	NOUN
ajst-25631	59	16	folding	fold	VERB
ajst-25631	59	17	precursors	precursor	NOUN
ajst-25631	59	18	and	and	CCONJ
ajst-25631	59	19	inclusion	inclusion	NOUN
ajst-25631	59	20	body	body	NOUN
ajst-25631	59	21	formation	formation	NOUN
ajst-25631	59	22	.	.	PUNCT
ajst-25631	60	1	the	the	DET
ajst-25631	60	2	use	use	NOUN
ajst-25631	60	3	of	of	ADP
ajst-25631	60	4	lower	low	ADJ
ajst-25631	60	5	concentrations	concentration	NOUN
ajst-25631	60	6	of	of	ADP
ajst-25631	60	7	inducers	inducer	NOUN
ajst-25631	60	8	can	can	AUX
ajst-25631	60	9	reduce	reduce	VERB
ajst-25631	60	10	the	the	DET
ajst-25631	60	11	cell	cell	NOUN
ajst-25631	60	12	burden	burden	NOUN
ajst-25631	60	13	,	,	PUNCT
ajst-25631	60	14	thereby	thereby	ADV
ajst-25631	60	15	increasing	increase	VERB
ajst-25631	60	16	the	the	DET
ajst-25631	60	17	solubility	solubility	NOUN
ajst-25631	60	18	and	and	CCONJ
ajst-25631	60	19	expression	expression	NOUN
ajst-25631	60	20	of	of	ADP
ajst-25631	60	21	the	the	DET
ajst-25631	60	22	target	target	NOUN
ajst-25631	60	23	protein	protein	NOUN
ajst-25631	60	24	.	.	PUNCT
ajst-25631	61	1	however	however	ADV
ajst-25631	61	2	,	,	PUNCT
ajst-25631	61	3	too	too	ADV
ajst-25631	61	4	low	low	ADJ
ajst-25631	61	5	concentration	concentration	NOUN
ajst-25631	61	6	may	may	AUX
ajst-25631	61	7	affect	affect	VERB
ajst-25631	61	8	protein	protein	NOUN
ajst-25631	61	9	production	production	NOUN
ajst-25631	61	10	.	.	PUNCT
ajst-25631	62	1	therefore	therefore	ADV
ajst-25631	62	2	,	,	PUNCT
ajst-25631	62	3	it	it	PRON
ajst-25631	62	4	is	be	AUX
ajst-25631	62	5	necessary	necessary	ADJ
ajst-25631	62	6	to	to	PART
ajst-25631	62	7	determine	determine	VERB
ajst-25631	62	8	the	the	DET
ajst-25631	62	9	optimal	optimal	ADJ
ajst-25631	62	10	iptg	iptg	NOUN
ajst-25631	62	11	concentration	concentration	NOUN
ajst-25631	62	12	by	by	ADP
ajst-25631	62	13	experiment	experiment	NOUN
ajst-25631	62	14	.	.	PUNCT
ajst-25631	63	1	in	in	ADP
ajst-25631	63	2	addition	addition	NOUN
ajst-25631	63	3	,	,	PUNCT
ajst-25631	63	4	in	in	ADP
ajst-25631	63	5	addition	addition	NOUN
ajst-25631	63	6	to	to	ADP
ajst-25631	63	7	iptg	iptg	PROPN
ajst-25631	63	8	,	,	PUNCT
ajst-25631	63	9	other	other	ADJ
ajst-25631	63	10	inducers	inducer	NOUN
ajst-25631	63	11	such	such	ADJ
ajst-25631	63	12	as	as	ADP
ajst-25631	63	13	lactose	lactose	NOUN
ajst-25631	63	14	,	,	PUNCT
ajst-25631	63	15	tryptophan	tryptophan	NOUN
ajst-25631	63	16	,	,	PUNCT
ajst-25631	63	17	and	and	CCONJ
ajst-25631	63	18	arabinose	arabinose	NOUN
ajst-25631	63	19	can	can	AUX
ajst-25631	63	20	also	also	ADV
ajst-25631	63	21	be	be	AUX
ajst-25631	63	22	considered	consider	VERB
ajst-25631	63	23	.	.	PUNCT
ajst-25631	64	1	lactose	lactose	ADJ
ajst-25631	64	2	autoinduction	autoinduction	NOUN
ajst-25631	64	3	has	have	VERB
ajst-25631	64	4	mild	mild	ADJ
ajst-25631	64	5	characteristics	characteristic	NOUN
ajst-25631	64	6	and	and	CCONJ
ajst-25631	64	7	is	be	AUX
ajst-25631	64	8	suitable	suitable	ADJ
ajst-25631	64	9	for	for	ADP
ajst-25631	64	10	the	the	DET
ajst-25631	64	11	efficient	efficient	ADJ
ajst-25631	64	12	expression	expression	NOUN
ajst-25631	64	13	of	of	ADP
ajst-25631	64	14	certain	certain	ADJ
ajst-25631	64	15	proteins	protein	NOUN
ajst-25631	64	16	.	.	PUNCT
ajst-25631	65	1	for	for	ADP
ajst-25631	65	2	example	example	NOUN
ajst-25631	65	3	,	,	PUNCT
ajst-25631	65	4	studies	study	NOUN
ajst-25631	65	5	have	have	AUX
ajst-25631	65	6	shown	show	VERB
ajst-25631	65	7	that	that	SCONJ
ajst-25631	65	8	when	when	SCONJ
ajst-25631	65	9	10	10	NUM
ajst-25631	65	10	%	%	NOUN
ajst-25631	65	11	lactose	lactose	NOUN
ajst-25631	65	12	is	be	AUX
ajst-25631	65	13	used	use	VERB
ajst-25631	65	14	as	as	ADP
ajst-25631	65	15	an	an	DET
ajst-25631	65	16	inducer	inducer	NOUN
ajst-25631	65	17	,	,	PUNCT
ajst-25631	65	18	it	it	PRON
ajst-25631	65	19	is	be	AUX
ajst-25631	65	20	suitable	suitable	ADJ
ajst-25631	65	21	for	for	ADP
ajst-25631	65	22	induction	induction	NOUN
ajst-25631	65	23	after	after	SCONJ
ajst-25631	65	24	the	the	DET
ajst-25631	65	25	culture	culture	NOUN
ajst-25631	65	26	reaches	reach	VERB
ajst-25631	65	27	a	a	DET
ajst-25631	65	28	specific	specific	ADJ
ajst-25631	65	29	optical	optical	ADJ
ajst-25631	65	30	density	density	NOUN
ajst-25631	65	31	(	(	PUNCT
ajst-25631	65	32	od	od	NOUN
ajst-25631	65	33	)	)	PUNCT
ajst-25631	65	34	,	,	PUNCT
ajst-25631	65	35	which	which	PRON
ajst-25631	65	36	can	can	AUX
ajst-25631	65	37	effectively	effectively	ADV
ajst-25631	65	38	improve	improve	VERB
ajst-25631	65	39	the	the	DET
ajst-25631	65	40	protein	protein	NOUN
ajst-25631	65	41	expression	expression	NOUN
ajst-25631	65	42	[	[	X
ajst-25631	65	43	21	21	NUM
ajst-25631	65	44	]	]	PUNCT
ajst-25631	65	45	.	.	PUNCT
ajst-25631	66	1	the	the	DET
ajst-25631	66	2	choice	choice	NOUN
ajst-25631	66	3	of	of	ADP
ajst-25631	66	4	induction	induction	NOUN
ajst-25631	66	5	time	time	NOUN
ajst-25631	66	6	is	be	AUX
ajst-25631	66	7	also	also	ADV
ajst-25631	66	8	very	very	ADV
ajst-25631	66	9	important	important	ADJ
ajst-25631	66	10	.	.	PUNCT
ajst-25631	67	1	in	in	ADP
ajst-25631	67	2	general	general	ADJ
ajst-25631	67	3	,	,	PUNCT
ajst-25631	67	4	induction	induction	NOUN
ajst-25631	67	5	should	should	AUX
ajst-25631	67	6	be	be	AUX
ajst-25631	67	7	carried	carry	VERB
ajst-25631	67	8	out	out	ADP
ajst-25631	67	9	during	during	ADP
ajst-25631	67	10	the	the	DET
ajst-25631	67	11	logarithmic	logarithmic	ADJ
ajst-25631	67	12	phase	phase	NOUN
ajst-25631	67	13	of	of	ADP
ajst-25631	67	14	bacterial	bacterial	ADJ
ajst-25631	67	15	growth	growth	NOUN
ajst-25631	67	16	,	,	PUNCT
ajst-25631	67	17	when	when	SCONJ
ajst-25631	67	18	the	the	DET
ajst-25631	67	19	cell	cell	NOUN
ajst-25631	67	20	activity	activity	NOUN
ajst-25631	67	21	is	be	AUX
ajst-25631	67	22	higher	high	ADJ
ajst-25631	67	23	and	and	CCONJ
ajst-25631	67	24	is	be	AUX
ajst-25631	67	25	better	well	ADV
ajst-25631	67	26	able	able	ADJ
ajst-25631	67	27	to	to	PART
ajst-25631	67	28	respond	respond	VERB
ajst-25631	67	29	to	to	ADP
ajst-25631	67	30	induction	induction	NOUN
ajst-25631	67	31	.	.	PUNCT
ajst-25631	68	1	for	for	ADP
ajst-25631	68	2	example	example	NOUN
ajst-25631	68	3	,	,	PUNCT
ajst-25631	68	4	induction	induction	NOUN
ajst-25631	68	5	at	at	ADP
ajst-25631	68	6	od	od	PROPN
ajst-25631	68	7	values	value	NOUN
ajst-25631	68	8	of	of	ADP
ajst-25631	68	9	1.0	1.0	NUM
ajst-25631	68	10	to	to	PART
ajst-25631	68	11	1.5	1.5	NUM
ajst-25631	68	12	can	can	AUX
ajst-25631	68	13	significantly	significantly	ADV
ajst-25631	68	14	improve	improve	VERB
ajst-25631	68	15	the	the	DET
ajst-25631	68	16	expression	expression	NOUN
ajst-25631	68	17	efficiency	efficiency	NOUN
ajst-25631	68	18	of	of	ADP
ajst-25631	68	19	recombinant	recombinant	ADJ
ajst-25631	68	20	proteins	protein	NOUN
ajst-25631	68	21	[	[	X
ajst-25631	68	22	22	22	NUM
ajst-25631	68	23	]	]	PUNCT
ajst-25631	68	24	.	.	PUNCT
ajst-25631	69	1	too	too	ADV
ajst-25631	69	2	early	early	ADJ
ajst-25631	69	3	or	or	CCONJ
ajst-25631	69	4	too	too	ADV
ajst-25631	69	5	late	late	ADJ
ajst-25631	69	6	induction	induction	NOUN
ajst-25631	69	7	can	can	AUX
ajst-25631	69	8	result	result	VERB
ajst-25631	69	9	in	in	ADP
ajst-25631	69	10	insufficient	insufficient	ADJ
ajst-25631	69	11	protein	protein	NOUN
ajst-25631	69	12	expression	expression	NOUN
ajst-25631	69	13	or	or	CCONJ
ajst-25631	69	14	the	the	DET
ajst-25631	69	15	formation	formation	NOUN
ajst-25631	69	16	of	of	ADP
ajst-25631	69	17	aggregates	aggregate	NOUN
ajst-25631	69	18	,	,	PUNCT
ajst-25631	69	19	which	which	PRON
ajst-25631	69	20	can	can	AUX
ajst-25631	69	21	affect	affect	VERB
ajst-25631	69	22	the	the	DET
ajst-25631	69	23	quality	quality	NOUN
ajst-25631	69	24	of	of	ADP
ajst-25631	69	25	the	the	DET
ajst-25631	69	26	final	final	ADJ
ajst-25631	69	27	product	product	NOUN
ajst-25631	69	28	.	.	PUNCT
ajst-25631	70	1	certain	certain	ADJ
ajst-25631	70	2	chemical	chemical	ADJ
ajst-25631	70	3	additives	additive	NOUN
ajst-25631	70	4	,	,	PUNCT
ajst-25631	70	5	such	such	ADJ
ajst-25631	70	6	as	as	ADP
ajst-25631	70	7	glycerin	glycerin	NOUN
ajst-25631	70	8	,	,	PUNCT
ajst-25631	70	9	trehalose	trehalose	INTJ
ajst-25631	70	10	,	,	PUNCT
ajst-25631	70	11	etc	etc	X
ajst-25631	70	12	.	.	X
ajst-25631	70	13	,	,	PUNCT
ajst-25631	70	14	can	can	AUX
ajst-25631	70	15	improve	improve	VERB
ajst-25631	70	16	intracellular	intracellular	ADJ
ajst-25631	70	17	viscosity	viscosity	NOUN
ajst-25631	70	18	and	and	CCONJ
ajst-25631	70	19	help	help	VERB
ajst-25631	70	20	improve	improve	VERB
ajst-25631	70	21	the	the	DET
ajst-25631	70	22	folding	fold	VERB
ajst-25631	70	23	state	state	NOUN
ajst-25631	70	24	of	of	ADP
ajst-25631	70	25	recombinant	recombinant	ADJ
ajst-25631	70	26	proteins	protein	NOUN
ajst-25631	70	27	.	.	PUNCT
ajst-25631	71	1	these	these	DET
ajst-25631	71	2	substances	substance	NOUN
ajst-25631	71	3	increase	increase	VERB
ajst-25631	71	4	the	the	DET
ajst-25631	71	5	production	production	NOUN
ajst-25631	71	6	of	of	ADP
ajst-25631	71	7	soluble	soluble	ADJ
ajst-25631	71	8	proteins	protein	NOUN
ajst-25631	71	9	by	by	ADP
ajst-25631	71	10	altering	alter	VERB
ajst-25631	71	11	the	the	DET
ajst-25631	71	12	microenvironment	microenvironment	NOUN
ajst-25631	71	13	within	within	ADP
ajst-25631	71	14	the	the	DET
ajst-25631	71	15	cell	cell	NOUN
ajst-25631	71	16	and	and	CCONJ
ajst-25631	71	17	reducing	reduce	VERB
ajst-25631	71	18	the	the	DET
ajst-25631	71	19	formation	formation	NOUN
ajst-25631	71	20	of	of	ADP
ajst-25631	71	21	aggregates	aggregate	NOUN
ajst-25631	71	22	[	[	X
ajst-25631	71	23	16,23	16,23	NOUN
ajst-25631	71	24	]	]	PUNCT
ajst-25631	71	25	.	.	PUNCT
ajst-25631	72	1	culture	culture	NOUN
ajst-25631	72	2	temperature	temperature	NOUN
ajst-25631	72	3	is	be	AUX
ajst-25631	72	4	another	another	DET
ajst-25631	72	5	important	important	ADJ
ajst-25631	72	6	factor	factor	NOUN
ajst-25631	72	7	affecting	affect	VERB
ajst-25631	72	8	protein	protein	NOUN
ajst-25631	72	9	expression	expression	NOUN
ajst-25631	72	10	.	.	PUNCT
ajst-25631	73	1	induction	induction	NOUN
ajst-25631	73	2	at	at	ADP
ajst-25631	73	3	lower	low	ADJ
ajst-25631	73	4	temperatures	temperature	NOUN
ajst-25631	73	5	generally	generally	ADV
ajst-25631	73	6	improves	improve	VERB
ajst-25631	73	7	the	the	DET
ajst-25631	73	8	solubility	solubility	NOUN
ajst-25631	73	9	of	of	ADP
ajst-25631	73	10	recombinant	recombinant	ADJ
ajst-25631	73	11	proteins	protein	NOUN
ajst-25631	73	12	and	and	CCONJ
ajst-25631	73	13	reduces	reduce	VERB
ajst-25631	73	14	the	the	DET
ajst-25631	73	15	formation	formation	NOUN
ajst-25631	73	16	of	of	ADP
ajst-25631	73	17	inclusion	inclusion	NOUN
ajst-25631	73	18	bodies	body	NOUN
ajst-25631	73	19	.	.	PUNCT
ajst-25631	74	1	lowering	lower	VERB
ajst-25631	74	2	the	the	DET
ajst-25631	74	3	induction	induction	NOUN
ajst-25631	74	4	temperature	temperature	NOUN
ajst-25631	74	5	contributes	contribute	VERB
ajst-25631	74	6	to	to	ADP
ajst-25631	74	7	the	the	DET
ajst-25631	74	8	metabolic	metabolic	NOUN
ajst-25631	74	9	burden	burden	NOUN
ajst-25631	74	10	of	of	ADP
ajst-25631	74	11	the	the	DET
ajst-25631	74	12	cell	cell	NOUN
ajst-25631	74	13	,	,	PUNCT
ajst-25631	74	14	which	which	PRON
ajst-25631	74	15	in	in	ADP
ajst-25631	74	16	turn	turn	NOUN
ajst-25631	74	17	promotes	promote	VERB
ajst-25631	74	18	the	the	DET
ajst-25631	74	19	correct	correct	ADJ
ajst-25631	74	20	folding	folding	NOUN
ajst-25631	74	21	and	and	CCONJ
ajst-25631	74	22	stability	stability	NOUN
ajst-25631	74	23	of	of	ADP
ajst-25631	74	24	the	the	DET
ajst-25631	74	25	target	target	NOUN
ajst-25631	74	26	protein	protein	NOUN
ajst-25631	74	27	[	[	X
ajst-25631	74	28	19,25	19,25	NUM
ajst-25631	74	29	]	]	PUNCT
ajst-25631	74	30	.	.	PUNCT
ajst-25631	75	1	certain	certain	ADJ
ajst-25631	75	2	proteins	protein	NOUN
ajst-25631	75	3	tend	tend	VERB
ajst-25631	75	4	to	to	PART
ajst-25631	75	5	aggregate	aggregate	VERB
ajst-25631	75	6	and	and	CCONJ
ajst-25631	75	7	dissolubilize	dissolubilize	VERB
ajst-25631	75	8	at	at	ADP
ajst-25631	75	9	37	37	NUM
ajst-25631	75	10	°	°	NOUN
ajst-25631	75	11	c	c	NOUN
ajst-25631	75	12	,	,	PUNCT
ajst-25631	75	13	and	and	CCONJ
ajst-25631	75	14	setting	set	VERB
ajst-25631	75	15	the	the	DET
ajst-25631	75	16	induction	induction	NOUN
ajst-25631	75	17	temperature	temperature	NOUN
ajst-25631	75	18	at	at	ADP
ajst-25631	75	19	30	30	NUM
ajst-25631	75	20	°	°	ADP
ajst-25631	75	21	c	c	NOUN
ajst-25631	75	22	can	can	AUX
ajst-25631	75	23	achieve	achieve	VERB
ajst-25631	75	24	better	well	ADJ
ajst-25631	75	25	expression	expression	NOUN
ajst-25631	75	26	results	result	NOUN
ajst-25631	75	27	in	in	ADP
ajst-25631	75	28	some	some	DET
ajst-25631	75	29	cases	case	NOUN
ajst-25631	75	30	,	,	PUNCT
ajst-25631	75	31	while	while	SCONJ
ajst-25631	75	32	reducing	reduce	VERB
ajst-25631	75	33	the	the	DET
ajst-25631	75	34	temperature	temperature	NOUN
ajst-25631	75	35	to	to	ADP
ajst-25631	75	36	18	18	NUM
ajst-25631	75	37	°	°	NOUN
ajst-25631	75	38	c	c	NOUN
ajst-25631	75	39	may	may	AUX
ajst-25631	75	40	also	also	ADV
ajst-25631	75	41	show	show	VERB
ajst-25631	75	42	better	well	ADJ
ajst-25631	75	43	results	result	NOUN
ajst-25631	75	44	in	in	ADP
ajst-25631	75	45	other	other	ADJ
ajst-25631	75	46	cases	case	NOUN
ajst-25631	75	47	.	.	PUNCT
ajst-25631	76	1	the	the	DET
ajst-25631	76	2	growth	growth	NOUN
ajst-25631	76	3	rate	rate	NOUN
ajst-25631	76	4	and	and	CCONJ
ajst-25631	76	5	expression	expression	NOUN
ajst-25631	76	6	level	level	NOUN
ajst-25631	76	7	of	of	ADP
ajst-25631	76	8	recombinant	recombinant	ADJ
ajst-25631	76	9	protein	protein	NOUN
ajst-25631	76	10	were	be	AUX
ajst-25631	76	11	affected	affect	VERB
ajst-25631	76	12	by	by	ADP
ajst-25631	76	13	different	different	ADJ
ajst-25631	76	14	medium	medium	ADJ
ajst-25631	76	15	components	component	NOUN
ajst-25631	76	16	.	.	PUNCT
ajst-25631	77	1	for	for	ADP
ajst-25631	77	2	example	example	NOUN
ajst-25631	77	3	,	,	PUNCT
ajst-25631	77	4	the	the	DET
ajst-25631	77	5	addition	addition	NOUN
ajst-25631	77	6	of	of	ADP
ajst-25631	77	7	certain	certain	ADJ
ajst-25631	77	8	amino	amino	ADJ
ajst-25631	77	9	acids	acid	NOUN
ajst-25631	77	10	or	or	CCONJ
ajst-25631	77	11	vitamins	vitamin	NOUN
ajst-25631	77	12	can	can	AUX
ajst-25631	77	13	promote	promote	VERB
ajst-25631	77	14	the	the	DET
ajst-25631	77	15	growth	growth	NOUN
ajst-25631	77	16	and	and	CCONJ
ajst-25631	77	17	metabolism	metabolism	NOUN
ajst-25631	77	18	of	of	ADP
ajst-25631	77	19	cells	cell	NOUN
ajst-25631	77	20	,	,	PUNCT
ajst-25631	77	21	thus	thus	ADV
ajst-25631	77	22	improving	improve	VERB
ajst-25631	77	23	the	the	DET
ajst-25631	77	24	expression	expression	NOUN
ajst-25631	77	25	effect	effect	NOUN
ajst-25631	77	26	of	of	ADP
ajst-25631	77	27	proteins	protein	NOUN
ajst-25631	77	28	.	.	PUNCT
ajst-25631	78	1	in	in	ADP
ajst-25631	78	2	addition	addition	NOUN
ajst-25631	78	3	,	,	PUNCT
ajst-25631	78	4	optimizing	optimize	VERB
ajst-25631	78	5	the	the	DET
ajst-25631	78	6	ratio	ratio	NOUN
ajst-25631	78	7	of	of	ADP
ajst-25631	78	8	carbon	carbon	NOUN
ajst-25631	78	9	and	and	CCONJ
ajst-25631	78	10	nitrogen	nitrogen	NOUN
ajst-25631	78	11	sources	source	NOUN
ajst-25631	78	12	in	in	ADP
ajst-25631	78	13	the	the	DET
ajst-25631	78	14	medium	medium	NOUN
ajst-25631	78	15	is	be	AUX
ajst-25631	78	16	also	also	ADV
ajst-25631	78	17	an	an	DET
ajst-25631	78	18	important	important	ADJ
ajst-25631	78	19	strategy	strategy	NOUN
ajst-25631	78	20	to	to	PART
ajst-25631	78	21	improve	improve	VERB
ajst-25631	78	22	protein	protein	NOUN
ajst-25631	78	23	expression	expression	NOUN
ajst-25631	78	24	[	[	X
ajst-25631	78	25	25	25	NUM
ajst-25631	78	26	]	]	PUNCT
ajst-25631	78	27	.	.	PUNCT
ajst-25631	79	1	4.3	4.3	NUM
ajst-25631	79	2	.	.	PUNCT
ajst-25631	79	3	pilot	pilot	NOUN
ajst-25631	79	4	test	test	NOUN
ajst-25631	79	5	optimization	optimization	NOUN
ajst-25631	79	6	it	it	PRON
ajst-25631	79	7	must	must	AUX
ajst-25631	79	8	be	be	AUX
ajst-25631	79	9	mentioned	mention	VERB
ajst-25631	79	10	that	that	SCONJ
ajst-25631	79	11	a	a	DET
ajst-25631	79	12	major	major	ADJ
ajst-25631	79	13	challenge	challenge	NOUN
ajst-25631	79	14	that	that	PRON
ajst-25631	79	15	laboratory	laboratory	NOUN
ajst-25631	79	16	research	research	NOUN
ajst-25631	79	17	results	result	NOUN
ajst-25631	79	18	must	must	AUX
ajst-25631	79	19	face	face	VERB
ajst-25631	79	20	is	be	AUX
ajst-25631	79	21	scale	scale	NOUN
ajst-25631	79	22	-	-	PUNCT
ajst-25631	79	23	up	up	ADP
ajst-25631	79	24	production	production	NOUN
ajst-25631	79	25	,	,	PUNCT
ajst-25631	79	26	which	which	PRON
ajst-25631	79	27	usually	usually	ADV
ajst-25631	79	28	needs	need	VERB
ajst-25631	79	29	to	to	PART
ajst-25631	79	30	follow	follow	VERB
ajst-25631	79	31	a	a	DET
ajst-25631	79	32	certain	certain	ADJ
ajst-25631	79	33	scale	scale	NOUN
ajst-25631	79	34	-	-	PUNCT
ajst-25631	79	35	up	up	ADP
ajst-25631	79	36	strategy	strategy	NOUN
ajst-25631	79	37	through	through	ADP
ajst-25631	79	38	pilot	pilot	NOUN
ajst-25631	79	39	tests	test	NOUN
ajst-25631	79	40	,	,	PUNCT
ajst-25631	79	41	optimize	optimize	VERB
ajst-25631	79	42	fermentation	fermentation	NOUN
ajst-25631	79	43	conditions	condition	NOUN
ajst-25631	79	44	according	accord	VERB
ajst-25631	79	45	to	to	ADP
ajst-25631	79	46	fermentation	fermentation	NOUN
ajst-25631	79	47	processes	process	NOUN
ajst-25631	79	48	and	and	CCONJ
ajst-25631	79	49	equipment	equipment	NOUN
ajst-25631	79	50	,	,	PUNCT
ajst-25631	79	51	and	and	CCONJ
ajst-25631	79	52	develop	develop	VERB
ajst-25631	79	53	225	225	NUM
ajst-25631	79	54	separation	separation	NOUN
ajst-25631	79	55	,	,	PUNCT
ajst-25631	79	56	purification	purification	NOUN
ajst-25631	79	57	,	,	PUNCT
ajst-25631	79	58	drying	dry	VERB
ajst-25631	79	59	and	and	CCONJ
ajst-25631	79	60	other	other	ADJ
ajst-25631	79	61	processes	process	NOUN
ajst-25631	79	62	in	in	ADP
ajst-25631	79	63	line	line	NOUN
ajst-25631	79	64	with	with	ADP
ajst-25631	79	65	large	large	ADJ
ajst-25631	79	66	-	-	PUNCT
ajst-25631	79	67	scale	scale	NOUN
ajst-25631	79	68	production	production	NOUN
ajst-25631	79	69	to	to	PART
ajst-25631	79	70	ensure	ensure	VERB
ajst-25631	79	71	that	that	SCONJ
ajst-25631	79	72	the	the	DET
ajst-25631	79	73	stability	stability	NOUN
ajst-25631	79	74	and	and	CCONJ
ajst-25631	79	75	consistency	consistency	NOUN
ajst-25631	79	76	of	of	ADP
ajst-25631	79	77	the	the	DET
ajst-25631	79	78	final	final	ADJ
ajst-25631	79	79	product	product	NOUN
ajst-25631	79	80	is	be	AUX
ajst-25631	79	81	maintained	maintain	VERB
ajst-25631	79	82	in	in	ADP
ajst-25631	79	83	reactors	reactor	NOUN
ajst-25631	79	84	of	of	ADP
ajst-25631	79	85	different	different	ADJ
ajst-25631	79	86	sizes	size	NOUN
ajst-25631	79	87	.	.	PUNCT
ajst-25631	80	1	5	5	X
ajst-25631	80	2	.	.	X
ajst-25631	80	3	conclusion	conclusion	NOUN
ajst-25631	80	4	escherichia	escherichia	NOUN
ajst-25631	80	5	coli	coli	NOUN
ajst-25631	80	6	has	have	AUX
ajst-25631	80	7	been	be	AUX
ajst-25631	80	8	widely	widely	ADV
ajst-25631	80	9	used	use	VERB
ajst-25631	80	10	in	in	ADP
ajst-25631	80	11	the	the	DET
ajst-25631	80	12	field	field	NOUN
ajst-25631	80	13	of	of	ADP
ajst-25631	80	14	recombinant	recombinant	ADJ
ajst-25631	80	15	protein	protein	NOUN
ajst-25631	80	16	,	,	PUNCT
ajst-25631	80	17	and	and	CCONJ
ajst-25631	80	18	as	as	ADP
ajst-25631	80	19	one	one	NUM
ajst-25631	80	20	of	of	ADP
ajst-25631	80	21	the	the	DET
ajst-25631	80	22	most	most	ADV
ajst-25631	80	23	commonly	commonly	ADV
ajst-25631	80	24	used	use	VERB
ajst-25631	80	25	expression	expression	NOUN
ajst-25631	80	26	systems	system	NOUN
ajst-25631	80	27	in	in	ADP
ajst-25631	80	28	genetic	genetic	ADJ
ajst-25631	80	29	engineering	engineering	NOUN
ajst-25631	80	30	,	,	PUNCT
ajst-25631	80	31	it	it	PRON
ajst-25631	80	32	has	have	AUX
ajst-25631	80	33	shown	show	VERB
ajst-25631	80	34	remarkable	remarkable	ADJ
ajst-25631	80	35	advantages	advantage	NOUN
ajst-25631	80	36	and	and	CCONJ
ajst-25631	80	37	potential	potential	NOUN
ajst-25631	80	38	.	.	PUNCT
ajst-25631	81	1	the	the	DET
ajst-25631	81	2	application	application	NOUN
ajst-25631	81	3	of	of	ADP
ajst-25631	81	4	e.	e.	PROPN
ajst-25631	81	5	coli	coli	PROPN
ajst-25631	81	6	in	in	ADP
ajst-25631	81	7	the	the	DET
ajst-25631	81	8	field	field	NOUN
ajst-25631	81	9	of	of	ADP
ajst-25631	81	10	recombinant	recombinant	ADJ
ajst-25631	81	11	proteins	protein	NOUN
ajst-25631	81	12	will	will	AUX
ajst-25631	81	13	continue	continue	VERB
ajst-25631	81	14	to	to	PART
ajst-25631	81	15	expand	expand	VERB
ajst-25631	81	16	and	and	CCONJ
ajst-25631	81	17	optimize	optimize	VERB
ajst-25631	81	18	.	.	PUNCT
ajst-25631	82	1	with	with	ADP
ajst-25631	82	2	the	the	DET
ajst-25631	82	3	introduction	introduction	NOUN
ajst-25631	82	4	of	of	ADP
ajst-25631	82	5	highthroughput	highthroughput	ADJ
ajst-25631	82	6	screening	screen	VERB
ajst-25631	82	7	technology	technology	NOUN
ajst-25631	82	8	,	,	PUNCT
ajst-25631	82	9	researchers	researcher	NOUN
ajst-25631	82	10	can	can	AUX
ajst-25631	82	11	more	more	ADV
ajst-25631	82	12	systematically	systematically	ADV
ajst-25631	82	13	explore	explore	VERB
ajst-25631	82	14	and	and	CCONJ
ajst-25631	82	15	determine	determine	VERB
ajst-25631	82	16	the	the	DET
ajst-25631	82	17	optimal	optimal	ADJ
ajst-25631	82	18	expression	expression	NOUN
ajst-25631	82	19	conditions	condition	NOUN
ajst-25631	82	20	to	to	PART
ajst-25631	82	21	further	far	ADV
ajst-25631	82	22	improve	improve	VERB
ajst-25631	82	23	protein	protein	NOUN
ajst-25631	82	24	production	production	NOUN
ajst-25631	82	25	and	and	CCONJ
ajst-25631	82	26	activity	activity	NOUN
ajst-25631	82	27	.	.	PUNCT
ajst-25631	83	1	at	at	ADP
ajst-25631	83	2	the	the	DET
ajst-25631	83	3	same	same	ADJ
ajst-25631	83	4	time	time	NOUN
ajst-25631	83	5	,	,	PUNCT
ajst-25631	83	6	the	the	DET
ajst-25631	83	7	limitations	limitation	NOUN
ajst-25631	83	8	of	of	ADP
ajst-25631	83	9	e.	e.	PROPN
ajst-25631	83	10	coli	coli	NOUN
ajst-25631	83	11	expression	expression	NOUN
ajst-25631	83	12	system	system	NOUN
ajst-25631	83	13	can	can	AUX
ajst-25631	83	14	be	be	AUX
ajst-25631	83	15	improved	improve	VERB
ajst-25631	83	16	through	through	ADP
ajst-25631	83	17	genetic	genetic	ADJ
ajst-25631	83	18	engineering	engineering	NOUN
ajst-25631	83	19	,	,	PUNCT
ajst-25631	83	20	expression	expression	NOUN
ajst-25631	83	21	vector	vector	NOUN
ajst-25631	83	22	optimization	optimization	NOUN
ajst-25631	83	23	and	and	CCONJ
ajst-25631	83	24	culture	culture	NOUN
ajst-25631	83	25	conditions	condition	NOUN
ajst-25631	83	26	adjustment	adjustment	NOUN
ajst-25631	83	27	.	.	PUNCT
ajst-25631	84	1	with	with	ADP
ajst-25631	84	2	the	the	DET
ajst-25631	84	3	continuous	continuous	ADJ
ajst-25631	84	4	progress	progress	NOUN
ajst-25631	84	5	and	and	CCONJ
ajst-25631	84	6	innovation	innovation	NOUN
ajst-25631	84	7	of	of	ADP
ajst-25631	84	8	technology	technology	NOUN
ajst-25631	84	9	,	,	PUNCT
ajst-25631	84	10	it	it	PRON
ajst-25631	84	11	will	will	AUX
ajst-25631	84	12	play	play	VERB
ajst-25631	84	13	a	a	DET
ajst-25631	84	14	more	more	ADV
ajst-25631	84	15	important	important	ADJ
ajst-25631	84	16	role	role	NOUN
ajst-25631	84	17	in	in	ADP
ajst-25631	84	18	the	the	DET
ajst-25631	84	19	development	development	NOUN
ajst-25631	84	20	of	of	ADP
ajst-25631	84	21	the	the	DET
ajst-25631	84	22	biomedical	biomedical	ADJ
ajst-25631	84	23	industry	industry	NOUN
ajst-25631	84	24	.	.	PUNCT
ajst-25631	85	1	author	author	NOUN
ajst-25631	85	2	contribution	contribution	NOUN
ajst-25631	85	3	statement	statement	NOUN
ajst-25631	85	4	all	all	DET
ajst-25631	85	5	authors	author	NOUN
ajst-25631	85	6	listed	list	VERB
ajst-25631	85	7	have	have	AUX
ajst-25631	85	8	significantly	significantly	ADV
ajst-25631	85	9	contributed	contribute	VERB
ajst-25631	85	10	to	to	ADP
ajst-25631	85	11	the	the	DET
ajst-25631	85	12	development	development	NOUN
ajst-25631	85	13	and	and	CCONJ
ajst-25631	85	14	the	the	DET
ajst-25631	85	15	writing	writing	NOUN
ajst-25631	85	16	of	of	ADP
ajst-25631	85	17	this	this	DET
ajst-25631	85	18	article	article	NOUN
ajst-25631	85	19	.	.	PUNCT
ajst-25631	86	1	declaration	declaration	NOUN
ajst-25631	86	2	of	of	ADP
ajst-25631	86	3	competing	compete	VERB
ajst-25631	86	4	interest	interest	NOUN
ajst-25631	86	5	the	the	DET
ajst-25631	86	6	authors	author	NOUN
ajst-25631	86	7	declare	declare	VERB
ajst-25631	86	8	that	that	SCONJ
ajst-25631	86	9	they	they	PRON
ajst-25631	86	10	have	have	VERB
ajst-25631	86	11	no	no	DET
ajst-25631	86	12	known	know	VERB
ajst-25631	86	13	competing	compete	VERB
ajst-25631	86	14	financial	financial	ADJ
ajst-25631	86	15	interests	interest	NOUN
ajst-25631	86	16	or	or	CCONJ
ajst-25631	86	17	personal	personal	ADJ
ajst-25631	86	18	relationships	relationship	NOUN
ajst-25631	86	19	that	that	PRON
ajst-25631	86	20	could	could	AUX
ajst-25631	86	21	have	have	AUX
ajst-25631	86	22	appeared	appear	VERB
ajst-25631	86	23	to	to	PART
ajst-25631	86	24	influence	influence	VERB
ajst-25631	86	25	the	the	DET
ajst-25631	86	26	work	work	NOUN
ajst-25631	86	27	reported	report	VERB
ajst-25631	86	28	in	in	ADP
ajst-25631	86	29	this	this	DET
ajst-25631	86	30	paper	paper	NOUN
ajst-25631	86	31	.	.	PUNCT
ajst-25631	87	1	acknowledgments	acknowledgment	NOUN
ajst-25631	87	2	this	this	DET
ajst-25631	87	3	study	study	NOUN
ajst-25631	87	4	was	be	AUX
ajst-25631	87	5	supported	support	VERB
ajst-25631	87	6	by	by	ADP
ajst-25631	87	7	bloomage	bloomage	NOUN
ajst-25631	87	8	biotech	biotech	NOUN
ajst-25631	87	9	(	(	PUNCT
ajst-25631	87	10	tianjin	tianjin	PROPN
ajst-25631	87	11	)	)	PUNCT
ajst-25631	87	12	technology	technology	PROPN
ajst-25631	87	13	co.	co.	PROPN
ajst-25631	87	14	,	,	PUNCT
ajst-25631	87	15	ltd	ltd	PROPN
ajst-25631	87	16	,	,	PUNCT
ajst-25631	87	17	project	project	NOUN
ajst-25631	87	18	24zycgsy00560	24zycgsy00560	NOUN
ajst-25631	87	19	.	.	PUNCT
ajst-25631	88	1	references	reference	NOUN
ajst-25631	88	2	[	[	X
ajst-25631	88	3	1	1	NUM
ajst-25631	88	4	]	]	X
ajst-25631	88	5	hayat	hayat	PROPN
ajst-25631	88	6	smg	smg	PROPN
ajst-25631	88	7	,	,	PUNCT
ajst-25631	88	8	farahani	farahani	X
ajst-25631	88	9	n	n	CCONJ
ajst-25631	88	10	,	,	PUNCT
ajst-25631	88	11	golichenari	golichenari	PROPN
ajst-25631	88	12	b	b	NOUN
ajst-25631	88	13	,	,	PUNCT
ajst-25631	88	14	sahebkar	sahebkar	PROPN
ajst-25631	88	15	a.	a.	NOUN
ajst-25631	88	16	recombinant	recombinant	ADJ
ajst-25631	88	17	protein	protein	NOUN
ajst-25631	88	18	expression	expression	NOUN
ajst-25631	88	19	in	in	ADP
ajst-25631	88	20	escherichia	escherichia	NOUN
ajst-25631	88	21	coli	coli	NOUN
ajst-25631	88	22	(	(	PUNCT
ajst-25631	88	23	e.	e.	NOUN
ajst-25631	88	24	coli	coli	PROPN
ajst-25631	88	25	):	):	PUNCT
ajst-25631	88	26	what	what	PRON
ajst-25631	88	27	we	we	PRON
ajst-25631	88	28	need	need	VERB
ajst-25631	88	29	to	to	PART
ajst-25631	88	30	know	know	VERB
ajst-25631	88	31	.	.	PUNCT
ajst-25631	89	1	curr	curr	PROPN
ajst-25631	89	2	pharm	pharm	PROPN
ajst-25631	89	3	des	des	PROPN
ajst-25631	89	4	.	.	PROPN
ajst-25631	89	5	2018;24(6):718	2018;24(6):718	NUM
ajst-25631	89	6	-	-	SYM
ajst-25631	89	7	725	725	NUM
ajst-25631	89	8	.	.	PUNCT
ajst-25631	90	1	[	[	X
ajst-25631	90	2	2	2	X
ajst-25631	90	3	]	]	X
ajst-25631	90	4	ahmad	ahmad	PROPN
ajst-25631	90	5	i	i	PRON
ajst-25631	90	6	,	,	PUNCT
ajst-25631	90	7	nawaz	nawaz	ADJ
ajst-25631	90	8	n	n	CCONJ
ajst-25631	90	9	,	,	PUNCT
ajst-25631	90	10	darwesh	darwesh	PROPN
ajst-25631	90	11	nm	nm	PROPN
ajst-25631	90	12	,	,	PUNCT
ajst-25631	90	13	ur	ur	PROPN
ajst-25631	90	14	rahman	rahman	PROPN
ajst-25631	90	15	s	s	PROPN
ajst-25631	90	16	,	,	PUNCT
ajst-25631	90	17	mustafa	mustafa	PROPN
ajst-25631	90	18	mz	mz	PROPN
ajst-25631	90	19	,	,	PUNCT
ajst-25631	90	20	khan	khan	PROPN
ajst-25631	90	21	sb	sb	PROPN
ajst-25631	90	22	,	,	PUNCT
ajst-25631	90	23	patching	patch	VERB
ajst-25631	90	24	sg	sg	PRON
ajst-25631	90	25	.	.	PUNCT
ajst-25631	91	1	overcoming	overcome	VERB
ajst-25631	91	2	challenges	challenge	NOUN
ajst-25631	91	3	for	for	ADP
ajst-25631	91	4	amplified	amplify	VERB
ajst-25631	91	5	expression	expression	NOUN
ajst-25631	91	6	of	of	ADP
ajst-25631	91	7	recombinant	recombinant	ADJ
ajst-25631	91	8	proteins	protein	NOUN
ajst-25631	91	9	using	use	VERB
ajst-25631	91	10	escherichia	escherichia	NOUN
ajst-25631	91	11	coli	coli	NOUN
ajst-25631	91	12	.	.	PUNCT
ajst-25631	92	1	protein	protein	NOUN
ajst-25631	92	2	expr	expr	NOUN
ajst-25631	92	3	purif	purif	PROPN
ajst-25631	92	4	.	.	PUNCT
ajst-25631	93	1	2018	2018	NUM
ajst-25631	93	2	apr	apr	NOUN
ajst-25631	93	3	;	;	PUNCT
ajst-25631	93	4	144:12	144:12	NUM
ajst-25631	93	5	-	-	SYM
ajst-25631	93	6	18	18	NUM
ajst-25631	93	7	.	.	PUNCT
ajst-25631	94	1	[	[	X
ajst-25631	94	2	3	3	X
ajst-25631	94	3	]	]	X
ajst-25631	94	4	gopal	gopal	PROPN
ajst-25631	94	5	gj	gj	PROPN
ajst-25631	94	6	,	,	PUNCT
ajst-25631	94	7	kumar	kumar	PROPN
ajst-25631	94	8	a.	a.	NOUN
ajst-25631	94	9	strategies	strategy	NOUN
ajst-25631	94	10	for	for	ADP
ajst-25631	94	11	the	the	DET
ajst-25631	94	12	production	production	NOUN
ajst-25631	94	13	of	of	ADP
ajst-25631	94	14	recombinant	recombinant	ADJ
ajst-25631	94	15	protein	protein	NOUN
ajst-25631	94	16	in	in	ADP
ajst-25631	94	17	escherichia	escherichia	NOUN
ajst-25631	94	18	coli	coli	NOUN
ajst-25631	94	19	.	.	PUNCT
ajst-25631	95	1	protein	protein	NOUN
ajst-25631	95	2	j.	j.	PROPN
ajst-25631	95	3	2013	2013	NUM
ajst-25631	95	4	aug	aug	PROPN
ajst-25631	95	5	;	;	PUNCT
ajst-25631	95	6	32	32	NUM
ajst-25631	95	7	(	(	PUNCT
ajst-25631	95	8	6	6	NUM
ajst-25631	95	9	):	):	PUNCT
ajst-25631	95	10	419	419	NUM
ajst-25631	95	11	-	-	SYM
ajst-25631	95	12	425	425	NUM
ajst-25631	95	13	.	.	PUNCT
ajst-25631	96	1	[	[	X
ajst-25631	96	2	4	4	NUM
ajst-25631	96	3	]	]	PUNCT
ajst-25631	96	4	saroha	saroha	NOUN
ajst-25631	96	5	p	p	NOUN
ajst-25631	96	6	,	,	PUNCT
ajst-25631	96	7	patil	patil	PROPN
ajst-25631	96	8	rs	rs	PROPN
ajst-25631	96	9	,	,	PUNCT
ajst-25631	96	10	rathore	rathore	NOUN
ajst-25631	96	11	as	as	ADP
ajst-25631	96	12	.	.	PUNCT
ajst-25631	97	1	recent	recent	ADJ
ajst-25631	97	2	advancements	advancement	NOUN
ajst-25631	97	3	in	in	ADP
ajst-25631	97	4	soluble	soluble	ADJ
ajst-25631	97	5	expression	expression	NOUN
ajst-25631	97	6	of	of	ADP
ajst-25631	97	7	recombinant	recombinant	ADJ
ajst-25631	97	8	antibody	antibody	NOUN
ajst-25631	97	9	fragments	fragment	NOUN
ajst-25631	97	10	in	in	ADP
ajst-25631	97	11	microbial	microbial	ADJ
ajst-25631	97	12	host	host	NOUN
ajst-25631	97	13	systems	system	NOUN
ajst-25631	97	14	.	.	PUNCT
ajst-25631	98	1	prep	prep	NOUN
ajst-25631	98	2	biochem	biochem	PROPN
ajst-25631	98	3	biotechnol	biotechnol	NOUN
ajst-25631	98	4	.	.	PUNCT
ajst-25631	99	1	2024	2024	NUM
ajst-25631	99	2	aug	aug	PROPN
ajst-25631	99	3	28:1	28:1	NUM
ajst-25631	99	4	-	-	SYM
ajst-25631	99	5	10	10	NUM
ajst-25631	99	6	.	.	PUNCT
ajst-25631	100	1	[	[	X
ajst-25631	100	2	5	5	NUM
ajst-25631	100	3	]	]	PUNCT
ajst-25631	100	4	rosano	rosano	NOUN
ajst-25631	100	5	gl	gl	PROPN
ajst-25631	100	6	,	,	PUNCT
ajst-25631	100	7	ceccarelli	ceccarelli	PROPN
ajst-25631	100	8	ea	ea	NOUN
ajst-25631	100	9	.	.	PUNCT
ajst-25631	100	10	recombinant	recombinant	ADJ
ajst-25631	100	11	protein	protein	NOUN
ajst-25631	100	12	expression	expression	NOUN
ajst-25631	100	13	in	in	ADP
ajst-25631	100	14	escherichia	escherichia	NOUN
ajst-25631	100	15	coli	coli	NOUN
ajst-25631	100	16	:	:	PUNCT
ajst-25631	100	17	advances	advance	NOUN
ajst-25631	100	18	and	and	CCONJ
ajst-25631	100	19	challenges	challenge	NOUN
ajst-25631	100	20	.	.	PUNCT
ajst-25631	101	1	front	front	ADJ
ajst-25631	101	2	microbiol	microbiol	PROPN
ajst-25631	101	3	.	.	PUNCT
ajst-25631	102	1	2014	2014	NUM
ajst-25631	102	2	apr	apr	NOUN
ajst-25631	102	3	17	17	NUM
ajst-25631	102	4	;	;	PUNCT
ajst-25631	102	5	5:172	5:172	NUM
ajst-25631	102	6	.	.	PUNCT
ajst-25631	103	1	[	[	X
ajst-25631	103	2	6	6	NUM
ajst-25631	103	3	]	]	PUNCT
ajst-25631	103	4	zhang	zhang	PROPN
ajst-25631	103	5	zx	zx	PROPN
ajst-25631	103	6	,	,	PUNCT
ajst-25631	103	7	nong	nong	PROPN
ajst-25631	103	8	ft	ft	PROPN
ajst-25631	103	9	,	,	PUNCT
ajst-25631	103	10	wang	wang	PROPN
ajst-25631	103	11	yz	yz	PROPN
ajst-25631	103	12	,	,	PUNCT
ajst-25631	103	13	yan	yan	PROPN
ajst-25631	103	14	cx	cx	PROPN
ajst-25631	103	15	,	,	PUNCT
ajst-25631	103	16	gu	gu	PROPN
ajst-25631	103	17	y	y	PROPN
ajst-25631	103	18	,	,	PUNCT
ajst-25631	103	19	song	song	NOUN
ajst-25631	103	20	p	p	NOUN
ajst-25631	103	21	,	,	PUNCT
ajst-25631	103	22	sun	sun	PROPN
ajst-25631	103	23	xm	xm	PROPN
ajst-25631	103	24	.	.	PUNCT
ajst-25631	104	1	strategies	strategy	NOUN
ajst-25631	104	2	for	for	ADP
ajst-25631	104	3	efficient	efficient	ADJ
ajst-25631	104	4	production	production	NOUN
ajst-25631	104	5	of	of	ADP
ajst-25631	104	6	recombinant	recombinant	ADJ
ajst-25631	104	7	proteins	protein	NOUN
ajst-25631	104	8	in	in	ADP
ajst-25631	104	9	escherichia	escherichia	NOUN
ajst-25631	104	10	coli	coli	NOUN
ajst-25631	104	11	:	:	PUNCT
ajst-25631	104	12	alleviating	alleviate	VERB
ajst-25631	104	13	the	the	DET
ajst-25631	104	14	host	host	NOUN
ajst-25631	104	15	burden	burden	NOUN
ajst-25631	104	16	and	and	CCONJ
ajst-25631	104	17	enhancing	enhance	VERB
ajst-25631	104	18	protein	protein	NOUN
ajst-25631	104	19	activity	activity	NOUN
ajst-25631	104	20	.	.	PUNCT
ajst-25631	105	1	microb	microb	PROPN
ajst-25631	105	2	cell	cell	PROPN
ajst-25631	105	3	fact	fact	NOUN
ajst-25631	105	4	.	.	PUNCT
ajst-25631	106	1	2022	2022	NUM
ajst-25631	106	2	sep	sep	NOUN
ajst-25631	106	3	15;21(1):191	15;21(1):191	NUM
ajst-25631	106	4	.	.	PUNCT
ajst-25631	107	1	[	[	X
ajst-25631	107	2	7	7	X
ajst-25631	107	3	]	]	X
ajst-25631	107	4	huang	huang	PROPN
ajst-25631	107	5	cj	cj	PROPN
ajst-25631	107	6	,	,	PUNCT
ajst-25631	107	7	lin	lin	PROPN
ajst-25631	107	8	h	h	PROPN
ajst-25631	107	9	,	,	PUNCT
ajst-25631	107	10	yang	yang	PROPN
ajst-25631	107	11	x.	x.	PROPN
ajst-25631	107	12	industrial	industrial	PROPN
ajst-25631	107	13	production	production	NOUN
ajst-25631	107	14	of	of	ADP
ajst-25631	107	15	recombinant	recombinant	ADJ
ajst-25631	107	16	therapeutics	therapeutic	NOUN
ajst-25631	107	17	in	in	ADP
ajst-25631	107	18	escherichia	escherichia	NOUN
ajst-25631	107	19	coli	coli	NOUN
ajst-25631	107	20	and	and	CCONJ
ajst-25631	107	21	its	its	PRON
ajst-25631	107	22	recent	recent	ADJ
ajst-25631	107	23	advancements	advancement	NOUN
ajst-25631	107	24	.	.	PUNCT
ajst-25631	108	1	j	j	PROPN
ajst-25631	108	2	ind	ind	PROPN
ajst-25631	108	3	microbiol	microbiol	PROPN
ajst-25631	108	4	biotechnol	biotechnol	NOUN
ajst-25631	108	5	.	.	PUNCT
ajst-25631	109	1	2012	2012	NUM
ajst-25631	109	2	mar;39	mar;39	NOUN
ajst-25631	109	3	(	(	PUNCT
ajst-25631	109	4	3	3	NUM
ajst-25631	109	5	):	):	PUNCT
ajst-25631	109	6	383	383	NUM
ajst-25631	109	7	-	-	SYM
ajst-25631	109	8	399	399	NUM
ajst-25631	109	9	.	.	PUNCT
ajst-25631	110	1	[	[	X
ajst-25631	110	2	8	8	NUM
ajst-25631	110	3	]	]	X
ajst-25631	110	4	gupta	gupta	PROPN
ajst-25631	110	5	v	v	NOUN
ajst-25631	110	6	,	,	PUNCT
ajst-25631	110	7	sengupta	sengupta	PROPN
ajst-25631	110	8	m	m	PROPN
ajst-25631	110	9	,	,	PUNCT
ajst-25631	110	10	prakash	prakash	PROPN
ajst-25631	110	11	j	j	PROPN
ajst-25631	110	12	,	,	PUNCT
ajst-25631	110	13	tripathy	tripathy	PROPN
ajst-25631	110	14	bc	bc	PROPN
ajst-25631	110	15	.	.	PROPN
ajst-25631	110	16	production	production	NOUN
ajst-25631	110	17	of	of	ADP
ajst-25631	110	18	recombinant	recombinant	ADJ
ajst-25631	110	19	pharmaceutical	pharmaceutical	ADJ
ajst-25631	110	20	proteins	protein	NOUN
ajst-25631	110	21	.	.	PUNCT
ajst-25631	111	1	basic	basic	ADJ
ajst-25631	111	2	and	and	CCONJ
ajst-25631	111	3	applied	applied	ADJ
ajst-25631	111	4	aspects	aspect	NOUN
ajst-25631	111	5	of	of	ADP
ajst-25631	111	6	biotechnology	biotechnology	NOUN
ajst-25631	111	7	.	.	PUNCT
ajst-25631	112	1	2016	2016	NUM
ajst-25631	112	2	oct	oct	NOUN
ajst-25631	112	3	23:77–101	23:77–101	NUM
ajst-25631	112	4	.	.	PUNCT
ajst-25631	113	1	[	[	X
ajst-25631	113	2	9	9	NUM
ajst-25631	113	3	]	]	PUNCT
ajst-25631	113	4	rosano	rosano	PROPN
ajst-25631	113	5	gl	gl	PROPN
ajst-25631	113	6	,	,	PUNCT
ajst-25631	113	7	morales	morales	PROPN
ajst-25631	113	8	es	es	PROPN
ajst-25631	113	9	,	,	PUNCT
ajst-25631	113	10	ceccarelli	ceccarelli	PROPN
ajst-25631	113	11	ea	ea	NOUN
ajst-25631	113	12	.	.	PUNCT
ajst-25631	114	1	new	new	ADJ
ajst-25631	114	2	tools	tool	NOUN
ajst-25631	114	3	for	for	ADP
ajst-25631	114	4	recombinant	recombinant	ADJ
ajst-25631	114	5	protein	protein	NOUN
ajst-25631	114	6	production	production	NOUN
ajst-25631	114	7	in	in	ADP
ajst-25631	114	8	escherichia	escherichia	NOUN
ajst-25631	114	9	coli	coli	NOUN
ajst-25631	114	10	:	:	PUNCT
ajst-25631	114	11	a	a	DET
ajst-25631	114	12	5	5	NUM
ajst-25631	114	13	-	-	PUNCT
ajst-25631	114	14	year	year	NOUN
ajst-25631	114	15	update	update	NOUN
ajst-25631	114	16	.	.	PUNCT
ajst-25631	115	1	protein	protein	NOUN
ajst-25631	115	2	sci	sci	PROPN
ajst-25631	115	3	.	.	PROPN
ajst-25631	115	4	2019	2019	NUM
ajst-25631	116	1	aug;28(8):1412	aug;28(8):1412	PROPN
ajst-25631	116	2	-	-	PUNCT
ajst-25631	116	3	1422	1422	NUM
ajst-25631	116	4	.	.	PUNCT
ajst-25631	117	1	[	[	X
ajst-25631	117	2	10	10	NUM
ajst-25631	117	3	]	]	SYM
ajst-25631	117	4	i̇ncir	i̇ncir	PUNCT
ajst-25631	117	5	i̇	i̇	PROPN
ajst-25631	117	6	,	,	PUNCT
ajst-25631	117	7	kaplan	kaplan	PROPN
ajst-25631	117	8	ö.	ö.	PROPN
ajst-25631	117	9	escherichia	escherichia	NOUN
ajst-25631	117	10	coli	coli	NOUN
ajst-25631	117	11	as	as	ADP
ajst-25631	117	12	a	a	DET
ajst-25631	117	13	versatile	versatile	ADJ
ajst-25631	117	14	cell	cell	NOUN
ajst-25631	117	15	factory	factory	NOUN
ajst-25631	117	16	:	:	PUNCT
ajst-25631	117	17	advances	advance	NOUN
ajst-25631	117	18	and	and	CCONJ
ajst-25631	117	19	challenges	challenge	NOUN
ajst-25631	117	20	in	in	ADP
ajst-25631	117	21	recombinant	recombinant	ADJ
ajst-25631	117	22	protein	protein	NOUN
ajst-25631	117	23	production	production	NOUN
ajst-25631	117	24	.	.	PUNCT
ajst-25631	118	1	protein	protein	NOUN
ajst-25631	118	2	expr	expr	NOUN
ajst-25631	118	3	purif	purif	PROPN
ajst-25631	118	4	.	.	PUNCT
ajst-25631	119	1	2024	2024	NUM
ajst-25631	119	2	jul	jul	PROPN
ajst-25631	119	3	;	;	PUNCT
ajst-25631	119	4	219:106463	219:106463	NUM
ajst-25631	119	5	.	.	PUNCT
ajst-25631	120	1	[	[	X
ajst-25631	120	2	11	11	NUM
ajst-25631	120	3	]	]	X
ajst-25631	120	4	singh	singh	PROPN
ajst-25631	120	5	a	a	X
ajst-25631	120	6	,	,	PUNCT
ajst-25631	120	7	upadhyay	upadhyay	PROPN
ajst-25631	120	8	v	v	PROPN
ajst-25631	120	9	,	,	PUNCT
ajst-25631	120	10	upadhyay	upadhyay	PROPN
ajst-25631	120	11	ak	ak	PROPN
ajst-25631	120	12	,	,	PUNCT
ajst-25631	120	13	singh	singh	PROPN
ajst-25631	120	14	sm	sm	PROPN
ajst-25631	120	15	,	,	PUNCT
ajst-25631	120	16	panda	panda	NOUN
ajst-25631	120	17	ak	ak	PROPN
ajst-25631	120	18	.	.	PROPN
ajst-25631	120	19	protein	protein	NOUN
ajst-25631	120	20	recovery	recovery	NOUN
ajst-25631	120	21	from	from	ADP
ajst-25631	120	22	inclusion	inclusion	NOUN
ajst-25631	120	23	bodies	body	NOUN
ajst-25631	120	24	of	of	ADP
ajst-25631	120	25	escherichia	escherichia	NOUN
ajst-25631	120	26	coli	coli	NOUN
ajst-25631	120	27	using	use	VERB
ajst-25631	120	28	mild	mild	ADJ
ajst-25631	120	29	solubilization	solubilization	NOUN
ajst-25631	120	30	process	process	NOUN
ajst-25631	120	31	.	.	PUNCT
ajst-25631	121	1	microb	microb	PROPN
ajst-25631	121	2	cell	cell	PROPN
ajst-25631	121	3	fact	fact	NOUN
ajst-25631	121	4	.	.	PUNCT
ajst-25631	122	1	2015	2015	NUM
ajst-25631	122	2	mar	mar	PROPN
ajst-25631	122	3	25	25	NUM
ajst-25631	122	4	;	;	PUNCT
ajst-25631	122	5	14:41	14:41	NUM
ajst-25631	122	6	.	.	PUNCT
ajst-25631	123	1	[	[	X
ajst-25631	123	2	12	12	NUM
ajst-25631	123	3	]	]	X
ajst-25631	123	4	baneyx	baneyx	PROPN
ajst-25631	123	5	f.	f.	PROPN
ajst-25631	123	6	recombinant	recombinant	ADJ
ajst-25631	123	7	protein	protein	NOUN
ajst-25631	123	8	expression	expression	NOUN
ajst-25631	123	9	in	in	ADP
ajst-25631	123	10	escherichia	escherichia	NOUN
ajst-25631	123	11	coli	coli	NOUN
ajst-25631	123	12	.	.	PUNCT
ajst-25631	124	1	curr	curr	PROPN
ajst-25631	124	2	opin	opin	PROPN
ajst-25631	124	3	biotechnol	biotechnol	NOUN
ajst-25631	124	4	.	.	PUNCT
ajst-25631	125	1	1999	1999	NUM
ajst-25631	126	1	oct;10(5):411	oct;10(5):411	NUM
ajst-25631	126	2	-	-	SYM
ajst-25631	126	3	421	421	NUM
ajst-25631	126	4	.	.	PUNCT
ajst-25631	127	1	[	[	X
ajst-25631	127	2	13	13	NUM
ajst-25631	127	3	]	]	X
ajst-25631	127	4	georgiou	georgiou	PROPN
ajst-25631	127	5	g	g	PROPN
ajst-25631	127	6	,	,	PUNCT
ajst-25631	127	7	valax	valax	ADJ
ajst-25631	127	8	p.	p.	NOUN
ajst-25631	127	9	expression	expression	NOUN
ajst-25631	127	10	of	of	ADP
ajst-25631	127	11	correctly	correctly	ADV
ajst-25631	127	12	folded	fold	VERB
ajst-25631	127	13	proteins	protein	NOUN
ajst-25631	127	14	in	in	ADP
ajst-25631	127	15	escherichia	escherichia	NOUN
ajst-25631	127	16	coli	coli	NOUN
ajst-25631	127	17	.	.	PUNCT
ajst-25631	128	1	curr	curr	PROPN
ajst-25631	128	2	opin	opin	PROPN
ajst-25631	128	3	biotechnol	biotechnol	NOUN
ajst-25631	128	4	.	.	PUNCT
ajst-25631	128	5	1996	1996	NUM
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ajst-25631	128	7	.	.	PUNCT
ajst-25631	129	1	[	[	X
ajst-25631	129	2	14	14	NUM
ajst-25631	129	3	]	]	PUNCT
ajst-25631	129	4	falak	falak	NOUN
ajst-25631	129	5	s	s	NOUN
ajst-25631	129	6	,	,	PUNCT
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ajst-25631	129	8	m	m	PROPN
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ajst-25631	129	10	rashid	rashid	PROPN
ajst-25631	129	11	n.	n.	PROPN
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ajst-25631	129	15	soluble	soluble	ADJ
ajst-25631	129	16	production	production	NOUN
ajst-25631	129	17	of	of	ADP
ajst-25631	129	18	heterologous	heterologous	ADJ
ajst-25631	129	19	proteins	protein	NOUN
ajst-25631	129	20	in	in	ADP
ajst-25631	129	21	escherichia	escherichia	NOUN
ajst-25631	129	22	coli	coli	NOUN
ajst-25631	129	23	.	.	PUNCT
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ajst-25631	130	2	(	(	PUNCT
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ajst-25631	131	5	fd	fd	PROPN
ajst-25631	131	6	,	,	PUNCT
ajst-25631	131	7	schroeder	schroeder	PROPN
ajst-25631	131	8	k	k	PROPN
ajst-25631	131	9	,	,	PUNCT
ajst-25631	131	10	jonas	jonas	PROPN
ajst-25631	131	11	k.	k.	PROPN
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ajst-25631	131	13	aggregation	aggregation	NOUN
ajst-25631	131	14	in	in	ADP
ajst-25631	131	15	bacteria	bacteria	NOUN
ajst-25631	131	16	.	.	PUNCT
ajst-25631	132	1	fems	fems	PROPN
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ajst-25631	132	4	.	.	PROPN
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ajst-25631	132	8	-	-	SYM
ajst-25631	132	9	72	72	NUM
ajst-25631	132	10	.	.	PUNCT
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ajst-25631	133	7	,	,	PUNCT
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ajst-25631	133	24	in	in	ADP
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ajst-25631	133	37	.	.	PUNCT
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ajst-25631	134	4	.	.	PROPN
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ajst-25631	135	2	winter;10	winter;10	NOUN
ajst-25631	135	3	(	(	PUNCT
ajst-25631	135	4	4	4	NUM
ajst-25631	135	5	):	):	PUNCT
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ajst-25631	135	7	-	-	SYM
ajst-25631	135	8	339	339	NUM
ajst-25631	135	9	.	.	PUNCT
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ajst-25631	136	2	17	17	NUM
ajst-25631	136	3	]	]	PUNCT
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ajst-25631	136	5	lm	lm	PROPN
ajst-25631	136	6	,	,	PUNCT
ajst-25631	136	7	hernández	hernández	PROPN
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ajst-25631	136	9	,	,	PUNCT
ajst-25631	136	10	rivero	rivero	PROPN
ajst-25631	136	11	em	em	PRON
ajst-25631	136	12	,	,	PUNCT
ajst-25631	136	13	barba	barba	PROPN
ajst-25631	136	14	de	de	PROPN
ajst-25631	136	15	la	la	PROPN
ajst-25631	136	16	rosa	rosa	PROPN
ajst-25631	136	17	ap	ap	PROPN
ajst-25631	136	18	,	,	PUNCT
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ajst-25631	136	20	jl	jl	PROPN
ajst-25631	136	21	,	,	PUNCT
ajst-25631	136	22	acevedo	acevedo	PROPN
ajst-25631	136	23	lg	lg	PROPN
ajst-25631	136	24	,	,	PUNCT
ajst-25631	136	25	de	de	X
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ajst-25631	136	28	a.	a.	NOUN
ajst-25631	136	29	optimization	optimization	NOUN
ajst-25631	136	30	of	of	ADP
ajst-25631	136	31	culture	culture	NOUN
ajst-25631	136	32	conditions	condition	NOUN
ajst-25631	136	33	for	for	ADP
ajst-25631	136	34	a	a	DET
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ajst-25631	136	36	gene	gene	NOUN
ajst-25631	136	37	expression	expression	NOUN
ajst-25631	136	38	in	in	ADP
ajst-25631	136	39	escherichia	escherichia	NOUN
ajst-25631	136	40	coli	coli	NOUN
ajst-25631	136	41	using	use	VERB
ajst-25631	136	42	response	response	NOUN
ajst-25631	136	43	surface	surface	NOUN
ajst-25631	136	44	methodology	methodology	NOUN
ajst-25631	136	45	:	:	PUNCT
ajst-25631	136	46	the	the	DET
ajst-25631	136	47	case	case	NOUN
ajst-25631	136	48	of	of	ADP
ajst-25631	136	49	human	human	ADJ
ajst-25631	136	50	interferon	interferon	PROPN
ajst-25631	136	51	beta	beta	NOUN
ajst-25631	136	52	.	.	PUNCT
ajst-25631	137	1	biomol	biomol	PROPN
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ajst-25631	137	3	.	.	PROPN
ajst-25631	137	4	2007	2007	NUM
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ajst-25631	137	6	-	-	PUNCT
ajst-25631	137	7	222	222	NUM
ajst-25631	137	8	.	.	PUNCT
ajst-25631	138	1	[	[	X
ajst-25631	138	2	18	18	NUM
ajst-25631	138	3	]	]	X
ajst-25631	138	4	valente	valente	NOUN
ajst-25631	138	5	ca	ca	PROPN
ajst-25631	138	6	,	,	PUNCT
ajst-25631	138	7	prazeres	prazere	NOUN
ajst-25631	138	8	dm	dm	PROPN
ajst-25631	138	9	,	,	PUNCT
ajst-25631	138	10	cabral	cabral	PROPN
ajst-25631	138	11	jm	jm	PROPN
ajst-25631	138	12	,	,	PUNCT
ajst-25631	138	13	monteiro	monteiro	PROPN
ajst-25631	138	14	ga	ga	PROPN
ajst-25631	138	15	.	.	PUNCT
ajst-25631	138	16	translational	translational	PROPN
ajst-25631	138	17	features	feature	NOUN
ajst-25631	138	18	of	of	ADP
ajst-25631	138	19	human	human	ADJ
ajst-25631	138	20	alpha	alpha	NOUN
ajst-25631	138	21	2b	2b	PART
ajst-25631	138	22	interferon	interferon	ADJ
ajst-25631	138	23	production	production	NOUN
ajst-25631	138	24	in	in	ADP
ajst-25631	138	25	escherichia	escherichia	NOUN
ajst-25631	138	26	coli	coli	NOUN
ajst-25631	138	27	.	.	PUNCT
ajst-25631	139	1	appl	appl	PROPN
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ajst-25631	139	4	.	.	PUNCT
ajst-25631	140	1	2004	2004	NUM
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ajst-25631	140	3	(	(	PUNCT
ajst-25631	140	4	8)	8)	NUM
ajst-25631	140	5	:	:	PUNCT
ajst-25631	140	6	5033	5033	NUM
ajst-25631	140	7	-	-	SYM
ajst-25631	140	8	5036	5036	NUM
ajst-25631	140	9	.	.	PUNCT
ajst-25631	141	1	[	[	X
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ajst-25631	141	9	,	,	PUNCT
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ajst-25631	141	11	j.	j.	PROPN
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ajst-25631	141	13	for	for	ADP
ajst-25631	141	14	optimization	optimization	NOUN
ajst-25631	141	15	of	of	ADP
ajst-25631	141	16	heterologous	heterologous	ADJ
ajst-25631	141	17	protein	protein	NOUN
ajst-25631	141	18	expression	expression	NOUN
ajst-25631	141	19	in	in	ADP
ajst-25631	141	20	e.	e.	PROPN
ajst-25631	141	21	coli	coli	PROPN
ajst-25631	141	22	:	:	PUNCT
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ajst-25631	141	25	reinforcements	reinforcement	NOUN
ajst-25631	141	26	.	.	PUNCT
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ajst-25631	142	2	j	j	PROPN
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ajst-25631	142	5	.	.	PUNCT
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ajst-25631	143	3	;	;	PUNCT
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ajst-25631	143	6	822	822	NUM
ajst-25631	143	7	.	.	PUNCT
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ajst-25631	144	9	,	,	PUNCT
ajst-25631	144	10	bai	bai	PROPN
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ajst-25631	144	41	coli	coli	NOUN
ajst-25631	144	42	for	for	ADP
ajst-25631	144	43	efficient	efficient	ADJ
ajst-25631	144	44	assembly	assembly	NOUN
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ajst-25631	144	48	.	.	PUNCT
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ajst-25631	146	4	.	.	PUNCT
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ajst-25631	147	14	j.	j.	PROPN
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ajst-25631	147	16	recombinant	recombinant	ADJ
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ajst-25631	147	18	expression	expression	NOUN
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ajst-25631	147	21	induction	induction	NOUN
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ajst-25631	147	23	in	in	ADP
ajst-25631	147	24	microtiter	microtiter	ADJ
ajst-25631	147	25	plates	plate	NOUN
ajst-25631	147	26	at	at	ADP
ajst-25631	147	27	different	different	ADJ
ajst-25631	147	28	temperatures	temperature	NOUN
ajst-25631	147	29	.	.	PUNCT
ajst-25631	148	1	microb	microb	PROPN
ajst-25631	148	2	cell	cell	PROPN
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ajst-25631	148	4	.	.	PUNCT
ajst-25631	149	1	2017	2017	NUM
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ajst-25631	149	4	.	.	PUNCT
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ajst-25631	150	3	]	]	PUNCT
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ajst-25631	150	5	g	g	PROPN
ajst-25631	150	6	,	,	PUNCT
ajst-25631	150	7	rostami	rostami	VERB
ajst-25631	150	8	a	a	PRON
ajst-25631	150	9	,	,	PUNCT
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ajst-25631	150	34	of	of	ADP
ajst-25631	150	35	functional	functional	ADJ
ajst-25631	150	36	recombinant	recombinant	ADJ
ajst-25631	150	37	human	human	ADJ
ajst-25631	150	38	growth	growth	NOUN
ajst-25631	150	39	hormone	hormone	NOUN
ajst-25631	150	40	in	in	ADP
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ajst-25631	150	42	coli	coli	NOUN
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ajst-25631	150	44	modified	modify	VERB
ajst-25631	150	45	staphylococcal	staphylococcal	ADJ
ajst-25631	150	46	protein	protein	NOUN
ajst-25631	150	47	a	a	DET
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ajst-25631	150	50	.	.	PUNCT
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ajst-25631	152	4	.	.	PUNCT
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ajst-25631	153	3	]	]	X
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ajst-25631	153	6	,	,	PUNCT
ajst-25631	153	7	romier	romi	ADJ
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ajst-25631	153	11	escherichia	escherichia	NOUN
ajst-25631	153	12	coli	coli	NOUN
ajst-25631	153	13	:	:	PUNCT
ajst-25631	153	14	becoming	become	VERB
ajst-25631	153	15	faster	fast	ADJ
ajst-25631	153	16	and	and	CCONJ
ajst-25631	153	17	more	more	ADV
ajst-25631	153	18	complex	complex	ADJ
ajst-25631	153	19	.	.	PUNCT
ajst-25631	154	1	curr	curr	PROPN
ajst-25631	154	2	opin	opin	PROPN
ajst-25631	154	3	struct	struct	NOUN
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ajst-25631	154	5	.	.	PUNCT
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ajst-25631	155	3	-	-	PUNCT
ajst-25631	155	4	334	334	NUM
ajst-25631	155	5	.	.	PUNCT
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ajst-25631	156	2	24	24	NUM
ajst-25631	156	3	]	]	PUNCT
ajst-25631	156	4	bahreini	bahreini	PROPN
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ajst-25631	156	6	,	,	PUNCT
ajst-25631	156	7	aghaiypour	aghaiypour	PROPN
ajst-25631	156	8	k	k	PROPN
ajst-25631	156	9	,	,	PUNCT
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ajst-25631	156	15	,	,	PUNCT
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ajst-25631	156	18	,	,	PUNCT
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ajst-25631	156	21	.	.	PUNCT
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ajst-25631	157	8	protein	protein	NOUN
ajst-25631	157	9	expression	expression	NOUN
ajst-25631	157	10	in	in	ADP
ajst-25631	157	11	escherichia	escherichia	NOUN
ajst-25631	157	12	coli	coli	NOUN
ajst-25631	157	13	.	.	PUNCT
ajst-25631	158	1	prep	prep	NOUN
ajst-25631	158	2	biochem	biochem	PROPN
ajst-25631	158	3	biotechnol	biotechnol	NOUN
ajst-25631	158	4	.	.	PUNCT
ajst-25631	159	1	2014;44(5):510528	2014;44(5):510528	NUM
ajst-25631	159	2	.	.	PUNCT
ajst-25631	160	1	[	[	X
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ajst-25631	160	3	]	]	X
ajst-25631	160	4	zhou	zhou	PROPN
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ajst-25631	160	6	,	,	PUNCT
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ajst-25631	160	9	,	,	PUNCT
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ajst-25631	160	25	extracellular	extracellular	ADJ
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ajst-25631	160	29	proteins	protein	NOUN
ajst-25631	160	30	using	use	VERB
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ajst-25631	160	33	.	.	PUNCT
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ajst-25631	161	4	.	.	PUNCT
ajst-25631	162	1	2018	2018	NUM
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ajst-25631	162	3	):	):	PUNCT
ajst-25631	162	4	1545	1545	NUM
ajst-25631	162	5	-	-	SYM
ajst-25631	162	6	1556	1556	NUM
ajst-25631	162	7	.	.	PUNCT