id sid tid token lemma pos ajst-32233 1 1 1 1 NUM ajst-32233 1 2 preparation preparation NOUN ajst-32233 1 3 and and CCONJ ajst-32233 1 4 performance performance NOUN ajst-32233 1 5 of of ADP ajst-32233 1 6 acrylate acrylate ADJ ajst-32233 1 7 hydrogel hydrogel NOUN ajst-32233 1 8 nanocomposite nanocomposite PROPN ajst-32233 1 9 adjuvant adjuvant PROPN ajst-32233 1 10 lu lu PROPN ajst-32233 1 11 bai bai PROPN ajst-32233 1 12 1 1 NUM ajst-32233 1 13 , , PUNCT ajst-32233 1 14 mingguang mingguang PROPN ajst-32233 1 15 hu hu PROPN ajst-32233 1 16 1 1 NUM ajst-32233 1 17 , , PUNCT ajst-32233 1 18 haijun haijun PROPN ajst-32233 1 19 zhou zhou PROPN ajst-32233 1 20 1 1 NUM ajst-32233 1 21 , , PUNCT ajst-32233 1 22 xiaoqi xiaoqi PROPN ajst-32233 1 23 chen chen PROPN ajst-32233 1 24 1 1 NUM ajst-32233 1 25 , , PUNCT ajst-32233 1 26 fen fen PROPN ajst-32233 1 27 zhang zhang PROPN ajst-32233 1 28 1 1 NUM ajst-32233 1 29 , , PUNCT ajst-32233 1 30 hua hua PROPN ajst-32233 1 31 cheng cheng PROPN ajst-32233 1 32 2 2 PROPN ajst-32233 1 33 and and CCONJ ajst-32233 1 34 yantao yantao PROPN ajst-32233 1 35 li li PROPN ajst-32233 1 36 1 1 NUM ajst-32233 1 37 , , PUNCT ajst-32233 1 38 * * PUNCT ajst-32233 1 39 1 1 NUM ajst-32233 1 40 institute institute NOUN ajst-32233 1 41 of of ADP ajst-32233 1 42 energy energy NOUN ajst-32233 1 43 resources resource NOUN ajst-32233 1 44 , , PUNCT ajst-32233 1 45 hebei hebei PROPN ajst-32233 1 46 academy academy PROPN ajst-32233 1 47 of of ADP ajst-32233 1 48 sciences sciences PROPN ajst-32233 1 49 , , PUNCT ajst-32233 1 50 shijiazhuang shijiazhuang PROPN ajst-32233 1 51 , , PUNCT ajst-32233 1 52 hebei hebei PROPN ajst-32233 1 53 province province PROPN ajst-32233 1 54 , , PUNCT ajst-32233 1 55 china china PROPN ajst-32233 1 56 2 2 NUM ajst-32233 1 57 institute institute PROPN ajst-32233 1 58 of of ADP ajst-32233 1 59 biology biology NOUN ajst-32233 1 60 , , PUNCT ajst-32233 1 61 hebei hebei PROPN ajst-32233 1 62 academy academy PROPN ajst-32233 1 63 of of ADP ajst-32233 1 64 sciences sciences PROPN ajst-32233 1 65 , , PUNCT ajst-32233 1 66 shijiazhuang shijiazhuang PROPN ajst-32233 1 67 , , PUNCT ajst-32233 1 68 hebei hebei PROPN ajst-32233 1 69 province province PROPN ajst-32233 1 70 , , PUNCT ajst-32233 1 71 china china PROPN ajst-32233 1 72 * * PUNCT ajst-32233 1 73 corresponding correspond VERB ajst-32233 1 74 author author NOUN ajst-32233 1 75 : : PUNCT ajst-32233 1 76 yantao yantao PROPN ajst-32233 1 77 li li PROPN ajst-32233 1 78 abstract abstract PROPN ajst-32233 1 79 . . PUNCT ajst-32233 2 1 in in ADP ajst-32233 2 2 order order NOUN ajst-32233 2 3 to to PART ajst-32233 2 4 meet meet VERB ajst-32233 2 5 the the DET ajst-32233 2 6 urgent urgent ADJ ajst-32233 2 7 need need NOUN ajst-32233 2 8 of of ADP ajst-32233 2 9 developing develop VERB ajst-32233 2 10 novel novel ADJ ajst-32233 2 11 vaccine vaccine NOUN ajst-32233 2 12 adjuvants adjuvant NOUN ajst-32233 2 13 with with ADP ajst-32233 2 14 rapid rapid ADJ ajst-32233 2 15 development development NOUN ajst-32233 2 16 in in ADP ajst-32233 2 17 the the DET ajst-32233 2 18 field field NOUN ajst-32233 2 19 of of ADP ajst-32233 2 20 biomedicine biomedicine NOUN ajst-32233 2 21 , , PUNCT ajst-32233 2 22 a a DET ajst-32233 2 23 novel novel ADJ ajst-32233 2 24 acrylate acrylate NOUN ajst-32233 2 25 hydrogel hydrogel NOUN ajst-32233 2 26 nanocomposite nanocomposite NOUN ajst-32233 2 27 adjuvant adjuvant ADJ ajst-32233 2 28 with with ADP ajst-32233 2 29 biological biological ADJ ajst-32233 2 30 safety safety NOUN ajst-32233 2 31 and and CCONJ ajst-32233 2 32 cell cell NOUN ajst-32233 2 33 targeting target VERB ajst-32233 2 34 was be AUX ajst-32233 2 35 prepared prepare VERB ajst-32233 2 36 by by ADP ajst-32233 2 37 compounding compound VERB ajst-32233 2 38 polyacrylate polyacrylate ADJ ajst-32233 2 39 nanoemulsion nanoemulsion NOUN ajst-32233 2 40 , , PUNCT ajst-32233 2 41 which which PRON ajst-32233 2 42 can can AUX ajst-32233 2 43 form form VERB ajst-32233 2 44 antigen antigen ADJ ajst-32233 2 45 reservoir reservoir NOUN ajst-32233 2 46 , , PUNCT ajst-32233 2 47 with with ADP ajst-32233 2 48 tlr3 tlr3 NOUN ajst-32233 2 49 agonist agonist NOUN ajst-32233 2 50 poly poly ADJ ajst-32233 2 51 i i PRON ajst-32233 2 52 : : PUNCT ajst-32233 2 53 c. c. NOUN ajst-32233 2 54 the the DET ajst-32233 2 55 microscopic microscopic ADJ ajst-32233 2 56 morphology morphology NOUN ajst-32233 2 57 of of ADP ajst-32233 2 58 the the DET ajst-32233 2 59 composite composite ADJ ajst-32233 2 60 adjuvant adjuvant NOUN ajst-32233 2 61 was be AUX ajst-32233 2 62 observed observe VERB ajst-32233 2 63 , , PUNCT ajst-32233 2 64 and and CCONJ ajst-32233 2 65 its its PRON ajst-32233 2 66 particle particle NOUN ajst-32233 2 67 size size NOUN ajst-32233 2 68 , , PUNCT ajst-32233 2 69 zeta zeta NOUN ajst-32233 2 70 potential potential NOUN ajst-32233 2 71 , , PUNCT ajst-32233 2 72 and and CCONJ ajst-32233 2 73 rheological rheological ADJ ajst-32233 2 74 properties property NOUN ajst-32233 2 75 were be AUX ajst-32233 2 76 characterized characterize VERB ajst-32233 2 77 and and CCONJ ajst-32233 2 78 analyzed analyze VERB ajst-32233 2 79 . . PUNCT ajst-32233 3 1 the the DET ajst-32233 3 2 composite composite ADJ ajst-32233 3 3 adjuvant adjuvant NOUN ajst-32233 3 4 exhibited exhibit VERB ajst-32233 3 5 a a DET ajst-32233 3 6 particle particle NOUN ajst-32233 3 7 size size NOUN ajst-32233 3 8 of of ADP ajst-32233 3 9 less less ADJ ajst-32233 3 10 than than ADP ajst-32233 3 11 50 50 NUM ajst-32233 3 12 nm nm NOUN ajst-32233 3 13 and and CCONJ ajst-32233 3 14 a a DET ajst-32233 3 15 cell cell NOUN ajst-32233 3 16 viability viability NOUN ajst-32233 3 17 of of ADP ajst-32233 3 18 75.59 75.59 NUM ajst-32233 3 19 % % NOUN ajst-32233 3 20 at at ADP ajst-32233 3 21 poly poly ADJ ajst-32233 3 22 i i NOUN ajst-32233 3 23 : : PUNCT ajst-32233 3 24 c c NOUN ajst-32233 3 25 concentration concentration NOUN ajst-32233 3 26 of of ADP ajst-32233 3 27 10 10 NUM ajst-32233 3 28 μg μg NOUN ajst-32233 3 29 / / SYM ajst-32233 3 30 ml ml PROPN ajst-32233 3 31 , , PUNCT ajst-32233 3 32 showing show VERB ajst-32233 3 33 good good ADJ ajst-32233 3 34 biocompatibility biocompatibility NOUN ajst-32233 3 35 . . PUNCT ajst-32233 4 1 furthermore furthermore ADV ajst-32233 4 2 , , PUNCT ajst-32233 4 3 the the DET ajst-32233 4 4 composite composite ADJ ajst-32233 4 5 adjuvant adjuvant NOUN ajst-32233 4 6 showed show VERB ajst-32233 4 7 significant significant ADJ ajst-32233 4 8 cell cell NOUN ajst-32233 4 9 targeting target VERB ajst-32233 4 10 and and CCONJ ajst-32233 4 11 enhanced enhance VERB ajst-32233 4 12 antigen antigen ADJ ajst-32233 4 13 phagocytosis phagocytosis NOUN ajst-32233 4 14 capabilities capability NOUN ajst-32233 4 15 . . PUNCT ajst-32233 5 1 this this DET ajst-32233 5 2 study study NOUN ajst-32233 5 3 provides provide VERB ajst-32233 5 4 a a DET ajst-32233 5 5 new new ADJ ajst-32233 5 6 approach approach NOUN ajst-32233 5 7 for for ADP ajst-32233 5 8 developing develop VERB ajst-32233 5 9 composite composite ADJ ajst-32233 5 10 adjuvant adjuvant ADJ ajst-32233 5 11 systems system NOUN ajst-32233 5 12 with with ADP ajst-32233 5 13 synergistic synergistic ADJ ajst-32233 5 14 immune immune NOUN ajst-32233 5 15 - - PUNCT ajst-32233 5 16 enhancing enhance VERB ajst-32233 5 17 functions function NOUN ajst-32233 5 18 , , PUNCT ajst-32233 5 19 demonstrating demonstrate VERB ajst-32233 5 20 promising promising ADJ ajst-32233 5 21 application application NOUN ajst-32233 5 22 potential potential NOUN ajst-32233 5 23 . . PUNCT ajst-32233 6 1 keywords keyword NOUN ajst-32233 6 2 : : PUNCT ajst-32233 6 3 hydrogel hydrogel NOUN ajst-32233 6 4 ; ; PUNCT ajst-32233 6 5 nanocomposite nanocomposite NOUN ajst-32233 6 6 adjuvant adjuvant NOUN ajst-32233 6 7 ; ; PUNCT ajst-32233 6 8 agonist agonist NOUN ajst-32233 6 9 ; ; PUNCT ajst-32233 6 10 phagocytosis phagocytosis NOUN ajst-32233 6 11 . . PUNCT ajst-32233 7 1 1 1 X ajst-32233 7 2 . . X ajst-32233 7 3 introduction introduction NOUN ajst-32233 7 4 in in ADP ajst-32233 7 5 recent recent ADJ ajst-32233 7 6 years year NOUN ajst-32233 7 7 , , PUNCT ajst-32233 7 8 novel novel ADJ ajst-32233 7 9 biomedical biomedical ADJ ajst-32233 7 10 therapeutic therapeutic ADJ ajst-32233 7 11 strategies strategy NOUN ajst-32233 7 12 based base VERB ajst-32233 7 13 on on ADP ajst-32233 7 14 polymeric polymeric ADJ ajst-32233 7 15 materials material NOUN ajst-32233 7 16 have have AUX ajst-32233 7 17 attracted attract VERB ajst-32233 7 18 significant significant ADJ ajst-32233 7 19 attention attention NOUN ajst-32233 7 20 . . PUNCT ajst-32233 8 1 due due ADP ajst-32233 8 2 to to ADP ajst-32233 8 3 their their PRON ajst-32233 8 4 high high ADJ ajst-32233 8 5 molecular molecular ADJ ajst-32233 8 6 weight weight NOUN ajst-32233 8 7 , , PUNCT ajst-32233 8 8 structural structural ADJ ajst-32233 8 9 tunability tunability NOUN ajst-32233 8 10 , , PUNCT ajst-32233 8 11 and and CCONJ ajst-32233 8 12 multifunctionality multifunctionality NOUN ajst-32233 8 13 , , PUNCT ajst-32233 8 14 polymeric polymeric ADJ ajst-32233 8 15 materials material NOUN ajst-32233 8 16 can can AUX ajst-32233 8 17 be be AUX ajst-32233 8 18 used use VERB ajst-32233 8 19 to to PART ajst-32233 8 20 construct construct VERB ajst-32233 8 21 nano nano NOUN ajst-32233 8 22 - - PUNCT ajst-32233 8 23 structures structure NOUN ajst-32233 8 24 or or CCONJ ajst-32233 8 25 micro micro NOUN ajst-32233 8 26 - - NOUN ajst-32233 8 27 structures structure NOUN ajst-32233 8 28 with with ADP ajst-32233 8 29 excellent excellent ADJ ajst-32233 8 30 drug drug NOUN ajst-32233 8 31 - - PUNCT ajst-32233 8 32 loading loading NOUN ajst-32233 8 33 capacity capacity NOUN ajst-32233 8 34 and and CCONJ ajst-32233 8 35 targeted target VERB ajst-32233 8 36 delivery delivery NOUN ajst-32233 8 37 performance performance NOUN ajst-32233 8 38 , , PUNCT ajst-32233 8 39 making make VERB ajst-32233 8 40 them they PRON ajst-32233 8 41 indispensable indispensable ADJ ajst-32233 8 42 tools tool NOUN ajst-32233 8 43 for for ADP ajst-32233 8 44 disease disease NOUN ajst-32233 8 45 treatment treatment NOUN ajst-32233 8 46 and and CCONJ ajst-32233 8 47 the the DET ajst-32233 8 48 regulation regulation NOUN ajst-32233 8 49 of of ADP ajst-32233 8 50 biological biological ADJ ajst-32233 8 51 systems[1 systems[1 NOUN ajst-32233 8 52 , , PUNCT ajst-32233 8 53 2 2 NUM ajst-32233 8 54 ] ] PUNCT ajst-32233 8 55 . . PUNCT ajst-32233 9 1 in in ADP ajst-32233 9 2 the the DET ajst-32233 9 3 field field NOUN ajst-32233 9 4 of of ADP ajst-32233 9 5 vaccine vaccine NOUN ajst-32233 9 6 adjuvants adjuvant NOUN ajst-32233 9 7 , , PUNCT ajst-32233 9 8 acrylic acrylic ADJ ajst-32233 9 9 polymers polymer NOUN ajst-32233 9 10 have have AUX ajst-32233 9 11 been be AUX ajst-32233 9 12 widely widely ADV ajst-32233 9 13 used use VERB ajst-32233 9 14 in in ADP ajst-32233 9 15 biomedical biomedical ADJ ajst-32233 9 16 fields field NOUN ajst-32233 9 17 such such ADJ ajst-32233 9 18 as as ADP ajst-32233 9 19 drug drug NOUN ajst-32233 9 20 delivery delivery NOUN ajst-32233 9 21 , , PUNCT ajst-32233 9 22 sustained sustain VERB ajst-32233 9 23 - - PUNCT ajst-32233 9 24 release release NOUN ajst-32233 9 25 formulations formulation NOUN ajst-32233 9 26 and and CCONJ ajst-32233 9 27 vaccine vaccine NOUN ajst-32233 9 28 adjuvants adjuvant NOUN ajst-32233 9 29 due due ADP ajst-32233 9 30 to to ADP ajst-32233 9 31 their their PRON ajst-32233 9 32 excellent excellent ADJ ajst-32233 9 33 biocompatibility biocompatibility NOUN ajst-32233 9 34 and and CCONJ ajst-32233 9 35 safety[3 safety[3 NOUN ajst-32233 9 36 - - PUNCT ajst-32233 9 37 5 5 NUM ajst-32233 9 38 ] ] PUNCT ajst-32233 9 39 . . PUNCT ajst-32233 10 1 hydrogels hydrogel NOUN ajst-32233 10 2 formed form VERB ajst-32233 10 3 from from ADP ajst-32233 10 4 acrylates acrylate NOUN ajst-32233 10 5 can can AUX ajst-32233 10 6 create create VERB ajst-32233 10 7 an an DET ajst-32233 10 8 antigen antigen ADJ ajst-32233 10 9 depot depot NOUN ajst-32233 10 10 at at ADP ajst-32233 10 11 the the DET ajst-32233 10 12 injection injection NOUN ajst-32233 10 13 site site NOUN ajst-32233 10 14 . . PUNCT ajst-32233 11 1 the the DET ajst-32233 11 2 mesh mesh NOUN ajst-32233 11 3 structure structure NOUN ajst-32233 11 4 of of ADP ajst-32233 11 5 the the DET ajst-32233 11 6 hydrogel hydrogel NOUN ajst-32233 11 7 enables enable VERB ajst-32233 11 8 the the DET ajst-32233 11 9 slow slow ADJ ajst-32233 11 10 and and CCONJ ajst-32233 11 11 sustained sustained ADJ ajst-32233 11 12 release release NOUN ajst-32233 11 13 of of ADP ajst-32233 11 14 antigens antigen NOUN ajst-32233 11 15 , , PUNCT ajst-32233 11 16 prolonging prolong VERB ajst-32233 11 17 the the DET ajst-32233 11 18 exposure exposure NOUN ajst-32233 11 19 time time NOUN ajst-32233 11 20 between between ADP ajst-32233 11 21 the the DET ajst-32233 11 22 immune immune ADJ ajst-32233 11 23 system system NOUN ajst-32233 11 24 and and CCONJ ajst-32233 11 25 the the DET ajst-32233 11 26 antigens antigen NOUN ajst-32233 11 27 . . PUNCT ajst-32233 12 1 meanwhile meanwhile ADV ajst-32233 12 2 , , PUNCT ajst-32233 12 3 with with ADP ajst-32233 12 4 the the DET ajst-32233 12 5 rapid rapid ADJ ajst-32233 12 6 advancement advancement NOUN ajst-32233 12 7 of of ADP ajst-32233 12 8 modern modern ADJ ajst-32233 12 9 biomedical biomedical ADJ ajst-32233 12 10 technology technology NOUN ajst-32233 12 11 , , PUNCT ajst-32233 12 12 the the DET ajst-32233 12 13 efficacy efficacy NOUN ajst-32233 12 14 of of ADP ajst-32233 12 15 single single ADJ ajst-32233 12 16 adjuvants adjuvant NOUN ajst-32233 12 17 often often ADV ajst-32233 12 18 falls fall VERB ajst-32233 12 19 short short ADV ajst-32233 12 20 of of ADP ajst-32233 12 21 requirements requirement NOUN ajst-32233 12 22 . . PUNCT ajst-32233 13 1 as as ADP ajst-32233 13 2 a a DET ajst-32233 13 3 result result NOUN ajst-32233 13 4 , , PUNCT ajst-32233 13 5 adjuvant adjuvant ADJ ajst-32233 13 6 development development NOUN ajst-32233 13 7 is be AUX ajst-32233 13 8 increasingly increasingly ADV ajst-32233 13 9 shifting shift VERB ajst-32233 13 10 toward toward ADP ajst-32233 13 11 composite composite ADJ ajst-32233 13 12 formulations formulation NOUN ajst-32233 13 13 . . PUNCT ajst-32233 14 1 khandhar[6 khandhar[6 VERB ajst-32233 14 2 ] ] PUNCT ajst-32233 14 3 successfully successfully ADV ajst-32233 14 4 activated activate VERB ajst-32233 14 5 and and CCONJ ajst-32233 14 6 induced induce VERB ajst-32233 14 7 a a DET ajst-32233 14 8 strong strong ADJ ajst-32233 14 9 th1 th1 ADJ ajst-32233 14 10 - - PUNCT ajst-32233 14 11 type type NOUN ajst-32233 14 12 cellular cellular ADJ ajst-32233 14 13 immune immune ADJ ajst-32233 14 14 response response NOUN ajst-32233 14 15 by by ADP ajst-32233 14 16 compounding compound VERB ajst-32233 14 17 anionic anionic ADJ ajst-32233 14 18 polyacrylic polyacrylic NOUN ajst-32233 14 19 acid acid NOUN ajst-32233 14 20 and and CCONJ ajst-32233 14 21 alum alum ADJ ajst-32233 14 22 as as ADP ajst-32233 14 23 a a DET ajst-32233 14 24 compound compound NOUN ajst-32233 14 25 adjuvant adjuvant NOUN ajst-32233 14 26 . . PUNCT ajst-32233 15 1 immunopotentiators immunopotentiator NOUN ajst-32233 15 2 are be AUX ajst-32233 15 3 substances substance NOUN ajst-32233 15 4 that that PRON ajst-32233 15 5 can can AUX ajst-32233 15 6 regulate regulate VERB ajst-32233 15 7 and and CCONJ ajst-32233 15 8 stimulate stimulate VERB ajst-32233 15 9 the the DET ajst-32233 15 10 immune immune ADJ ajst-32233 15 11 system system NOUN ajst-32233 15 12 nonspecifically nonspecifically ADV ajst-32233 15 13 . . PUNCT ajst-32233 16 1 in in ADP ajst-32233 16 2 modern modern ADJ ajst-32233 16 3 vaccinology vaccinology NOUN ajst-32233 16 4 , , PUNCT ajst-32233 16 5 many many ADJ ajst-32233 16 6 scholars scholar NOUN ajst-32233 16 7 compounded compound VERB ajst-32233 16 8 cpg cpg NOUN ajst-32233 16 9 , , PUNCT ajst-32233 16 10 quila quila NOUN ajst-32233 16 11 or or CCONJ ajst-32233 16 12 other other ADJ ajst-32233 16 13 immunopotentiators immunopotentiator NOUN ajst-32233 16 14 into into ADP ajst-32233 16 15 compound compound NOUN ajst-32233 16 16 adjuvants adjuvant NOUN ajst-32233 16 17 , , PUNCT ajst-32233 16 18 and and CCONJ ajst-32233 16 19 studied study VERB ajst-32233 16 20 the the DET ajst-32233 16 21 effect effect NOUN ajst-32233 16 22 of of ADP ajst-32233 16 23 immunopotentiators immunopotentiator NOUN ajst-32233 16 24 on on ADP ajst-32233 16 25 adjuvant adjuvant ADJ ajst-32233 16 26 performance performance NOUN ajst-32233 16 27 . . PUNCT ajst-32233 17 1 chen chen PROPN ajst-32233 17 2 t.h t.h PROPN ajst-32233 17 3 et et PROPN ajst-32233 17 4 al.[7 al.[7 PROPN ajst-32233 17 5 ] ] PUNCT ajst-32233 17 6 combined combined ADJ ajst-32233 17 7 pelc pelc NOUN ajst-32233 17 8 , , PUNCT ajst-32233 17 9 a a DET ajst-32233 17 10 novel novel ADJ ajst-32233 17 11 nanoemulsion nanoemulsion NOUN ajst-32233 17 12 adjuvant adjuvant NOUN ajst-32233 17 13 composed compose VERB ajst-32233 17 14 of of ADP ajst-32233 17 15 biodegradable biodegradable ADJ ajst-32233 17 16 peg peg NOUN ajst-32233 17 17 - - PUNCT ajst-32233 17 18 b b NOUN ajst-32233 17 19 - - PUNCT ajst-32233 17 20 placl placl NOUN ajst-32233 17 21 , , PUNCT ajst-32233 17 22 span85 span85 NOUN ajst-32233 17 23 and and CCONJ ajst-32233 17 24 squalene squalene NOUN ajst-32233 17 25 , , PUNCT ajst-32233 17 26 with with ADP ajst-32233 17 27 cpg cpg NOUN ajst-32233 17 28 , , PUNCT ajst-32233 17 29 and and CCONJ ajst-32233 17 30 found find VERB ajst-32233 17 31 that that SCONJ ajst-32233 17 32 pelc pelc NOUN ajst-32233 17 33 / / SYM ajst-32233 17 34 cpg cpg NOUN ajst-32233 17 35 could could AUX ajst-32233 17 36 induce induce VERB ajst-32233 17 37 potent potent ADJ ajst-32233 17 38 immune immune ADJ ajst-32233 17 39 response response NOUN ajst-32233 17 40 , , PUNCT ajst-32233 17 41 induce induce VERB ajst-32233 17 42 more more ADV ajst-32233 17 43 specific specific ADJ ajst-32233 17 44 ifn ifn NOUN ajst-32233 17 45 - - PUNCT ajst-32233 17 46 γ γ NOUN ajst-32233 17 47 in in ADP ajst-32233 17 48 spleen spleen NOUN ajst-32233 17 49 , , PUNCT ajst-32233 17 50 and and CCONJ ajst-32233 17 51 improve improve VERB ajst-32233 17 52 the the DET ajst-32233 17 53 immune immune ADJ ajst-32233 17 54 capacity capacity NOUN ajst-32233 17 55 of of ADP ajst-32233 17 56 the the DET ajst-32233 17 57 body body NOUN ajst-32233 17 58 . . PUNCT ajst-32233 18 1 polyinosinic polyinosinic ADJ ajst-32233 18 2 ( ( PUNCT ajst-32233 18 3 poly poly ADJ ajst-32233 18 4 i i NOUN ajst-32233 18 5 : : PUNCT ajst-32233 18 6 c c X ajst-32233 18 7 ) ) PUNCT ajst-32233 18 8 is be AUX ajst-32233 18 9 a a DET ajst-32233 18 10 synthetic synthetic ADJ ajst-32233 18 11 dsrna dsrna NOUN ajst-32233 18 12 complex complex NOUN ajst-32233 18 13 . . PUNCT ajst-32233 19 1 as as ADP ajst-32233 19 2 a a DET ajst-32233 19 3 tlr3 tlr3 PROPN ajst-32233 19 4 agonist agonist NOUN ajst-32233 19 5 , , PUNCT ajst-32233 19 6 it it PRON ajst-32233 19 7 binds bind VERB ajst-32233 19 8 to to ADP ajst-32233 19 9 tlr3 tlr3 PROPN ajst-32233 19 10 receptors receptor NOUN ajst-32233 19 11 on on ADP ajst-32233 19 12 cells cell NOUN ajst-32233 19 13 , , PUNCT ajst-32233 19 14 which which PRON ajst-32233 19 15 are be AUX ajst-32233 19 16 widely widely ADV ajst-32233 19 17 expressed express VERB ajst-32233 19 18 on on ADP ajst-32233 19 19 dendritic dendritic ADJ ajst-32233 19 20 cells cell NOUN ajst-32233 19 21 , , PUNCT ajst-32233 19 22 t t NOUN ajst-32233 19 23 cells cell NOUN ajst-32233 19 24 , , PUNCT ajst-32233 19 25 natural natural ADJ ajst-32233 19 26 killer killer NOUN ajst-32233 19 27 cells cell NOUN ajst-32233 19 28 , , PUNCT ajst-32233 19 29 and and CCONJ ajst-32233 19 30 some some DET ajst-32233 19 31 non non ADJ ajst-32233 19 32 - - ADJ ajst-32233 19 33 immune immune ADJ ajst-32233 19 34 cells cell NOUN ajst-32233 19 35 , , PUNCT ajst-32233 19 36 thus thus ADV ajst-32233 19 37 mediating mediate VERB ajst-32233 19 38 innate innate ADJ ajst-32233 19 39 and and CCONJ ajst-32233 19 40 adaptive adaptive ADJ ajst-32233 19 41 immune immune ADJ ajst-32233 19 42 responses response NOUN ajst-32233 19 43 . . PUNCT ajst-32233 20 1 this this PRON ajst-32233 20 2 indicates indicate VERB ajst-32233 20 3 that that SCONJ ajst-32233 20 4 poly poly ADJ ajst-32233 20 5 i i PRON ajst-32233 20 6 : : PUNCT ajst-32233 20 7 c c PROPN ajst-32233 20 8 has have VERB ajst-32233 20 9 the the DET ajst-32233 20 10 potential potential NOUN ajst-32233 20 11 as as ADP ajst-32233 20 12 a a DET ajst-32233 20 13 vaccine vaccine NOUN ajst-32233 20 14 adjuvant[8 adjuvant[8 ADP ajst-32233 20 15 ] ] PUNCT ajst-32233 20 16 . . PUNCT ajst-32233 21 1 sun sun PROPN ajst-32233 21 2 et et PROPN ajst-32233 21 3 al.[9 al.[9 PROPN ajst-32233 21 4 ] ] PUNCT ajst-32233 21 5 formed form VERB ajst-32233 21 6 the the DET ajst-32233 21 7 composite composite ADJ ajst-32233 21 8 adjuvant adjuvant NOUN ajst-32233 21 9 al al PROPN ajst-32233 21 10 - - PUNCT ajst-32233 21 11 poly poly ADJ ajst-32233 22 1 i i NOUN ajst-32233 22 2 : : PUNCT ajst-32233 22 3 c c NOUN ajst-32233 22 4 by by ADP ajst-32233 22 5 covalently covalently ADV ajst-32233 22 6 binding bind VERB ajst-32233 22 7 the the DET ajst-32233 22 8 phosphate phosphate ADJ ajst-32233 22 9 group group NOUN ajst-32233 22 10 of of ADP ajst-32233 22 11 poly poly ADJ ajst-32233 22 12 i i PRON ajst-32233 22 13 : : PUNCT ajst-32233 22 14 c c NOUN ajst-32233 22 15 to to ADP ajst-32233 22 16 the the DET ajst-32233 22 17 hydroxyl hydroxyl NOUN ajst-32233 22 18 group group NOUN ajst-32233 22 19 of of ADP ajst-32233 22 20 2 2 NUM ajst-32233 22 21 the the DET ajst-32233 22 22 traditional traditional ADJ ajst-32233 22 23 adjuvant adjuvant ADJ ajst-32233 22 24 aluminum aluminum PROPN ajst-32233 22 25 oxyhydroxide oxyhydroxide NOUN ajst-32233 22 26 ( ( PUNCT ajst-32233 22 27 alooh alooh PROPN ajst-32233 22 28 ) ) PUNCT ajst-32233 22 29 . . PUNCT ajst-32233 23 1 the the DET ajst-32233 23 2 results result NOUN ajst-32233 23 3 found find VERB ajst-32233 23 4 that that SCONJ ajst-32233 23 5 al al PROPN ajst-32233 23 6 - - PUNCT ajst-32233 23 7 poly poly ADJ ajst-32233 23 8 i i NOUN ajst-32233 23 9 : : PUNCT ajst-32233 23 10 c c X ajst-32233 23 11 in in ADP ajst-32233 23 12 the the DET ajst-32233 23 13 hbv hbv NOUN ajst-32233 23 14 model model NOUN ajst-32233 23 15 can can AUX ajst-32233 23 16 significantly significantly ADV ajst-32233 23 17 increase increase VERB ajst-32233 23 18 the the DET ajst-32233 23 19 levels level NOUN ajst-32233 23 20 of of ADP ajst-32233 23 21 igg igg PROPN ajst-32233 23 22 , , PUNCT ajst-32233 23 23 igg1 igg1 NOUN ajst-32233 23 24 and and CCONJ ajst-32233 23 25 igg2c igg2c X ajst-32233 23 26 specific specific ADJ ajst-32233 23 27 antibodies antibody NOUN ajst-32233 23 28 in in ADP ajst-32233 23 29 serum serum NOUN ajst-32233 23 30 , , PUNCT ajst-32233 23 31 and and CCONJ ajst-32233 23 32 the the DET ajst-32233 23 33 number number NOUN ajst-32233 23 34 of of ADP ajst-32233 23 35 dendritic dendritic ADJ ajst-32233 23 36 cells cell NOUN ajst-32233 23 37 carrying carry VERB ajst-32233 23 38 the the DET ajst-32233 23 39 antigen antigen NOUN ajst-32233 23 40 also also ADV ajst-32233 23 41 increased increase VERB ajst-32233 23 42 by by ADP ajst-32233 23 43 2.5 2.5 NUM ajst-32233 23 44 % % NOUN ajst-32233 23 45 compared compare VERB ajst-32233 23 46 with with ADP ajst-32233 23 47 the the DET ajst-32233 23 48 antigen antigen PROPN ajst-32233 23 49 group group NOUN ajst-32233 23 50 . . PUNCT ajst-32233 24 1 the the DET ajst-32233 24 2 incorporation incorporation NOUN ajst-32233 24 3 of of ADP ajst-32233 24 4 immunopotentiators immunopotentiator NOUN ajst-32233 24 5 into into ADP ajst-32233 24 6 adjuvant adjuvant ADJ ajst-32233 24 7 systems system NOUN ajst-32233 24 8 can can AUX ajst-32233 24 9 produce produce VERB ajst-32233 24 10 a a DET ajst-32233 24 11 synergistic synergistic ADJ ajst-32233 24 12 effect effect NOUN ajst-32233 24 13 , , PUNCT ajst-32233 24 14 inducing induce VERB ajst-32233 24 15 a a DET ajst-32233 24 16 higher high ADJ ajst-32233 24 17 level level NOUN ajst-32233 24 18 of of ADP ajst-32233 24 19 immune immune ADJ ajst-32233 24 20 response response NOUN ajst-32233 24 21 in in ADP ajst-32233 24 22 the the DET ajst-32233 24 23 body body NOUN ajst-32233 24 24 . . PUNCT ajst-32233 25 1 therefore therefore ADV ajst-32233 25 2 , , PUNCT ajst-32233 25 3 composite composite ADJ ajst-32233 25 4 adjuvants adjuvant NOUN ajst-32233 25 5 prepared prepare VERB ajst-32233 25 6 by by ADP ajst-32233 25 7 combining combine VERB ajst-32233 25 8 polyacrylate polyacrylate NOUN ajst-32233 25 9 - - PUNCT ajst-32233 25 10 based base VERB ajst-32233 25 11 materials material NOUN ajst-32233 25 12 with with ADP ajst-32233 25 13 poly poly ADJ ajst-32233 25 14 i i NOUN ajst-32233 25 15 : : PUNCT ajst-32233 25 16 c c NOUN ajst-32233 25 17 deserves deserve VERB ajst-32233 25 18 in in ADP ajst-32233 25 19 - - PUNCT ajst-32233 25 20 depth depth NOUN ajst-32233 25 21 exploration exploration NOUN ajst-32233 25 22 and and CCONJ ajst-32233 25 23 development development NOUN ajst-32233 25 24 . . PUNCT ajst-32233 26 1 in in ADP ajst-32233 26 2 this this DET ajst-32233 26 3 project project NOUN ajst-32233 26 4 , , PUNCT ajst-32233 26 5 a a DET ajst-32233 26 6 polyacrylate polyacrylate ADJ ajst-32233 26 7 hydrogel hydrogel NOUN ajst-32233 26 8 nanocomposite nanocomposite NOUN ajst-32233 26 9 adjuvant adjuvant NOUN ajst-32233 26 10 was be AUX ajst-32233 26 11 fabricated fabricate VERB ajst-32233 26 12 by by ADP ajst-32233 26 13 formulating formulate VERB ajst-32233 26 14 poly poly ADJ ajst-32233 26 15 i i NOUN ajst-32233 26 16 : : PUNCT ajst-32233 26 17 c c NOUN ajst-32233 26 18 with with ADP ajst-32233 26 19 polyacrylate polyacrylate ADJ ajst-32233 26 20 hydrogel hydrogel NOUN ajst-32233 26 21 and and CCONJ ajst-32233 26 22 a a DET ajst-32233 26 23 lab lab NOUN ajst-32233 26 24 - - PUNCT ajst-32233 26 25 made make VERB ajst-32233 26 26 nanoemulsion nanoemulsion NOUN ajst-32233 26 27 . . PUNCT ajst-32233 27 1 the the DET ajst-32233 27 2 microstructure microstructure NOUN ajst-32233 27 3 , , PUNCT ajst-32233 27 4 particle particle NOUN ajst-32233 27 5 size size NOUN ajst-32233 27 6 , , PUNCT ajst-32233 27 7 rheological rheological ADJ ajst-32233 27 8 properties property NOUN ajst-32233 27 9 , , PUNCT ajst-32233 27 10 cytotoxicity cytotoxicity NOUN ajst-32233 27 11 , , PUNCT ajst-32233 27 12 and and CCONJ ajst-32233 27 13 cellular cellular ADJ ajst-32233 27 14 uptake uptake ADJ ajst-32233 27 15 capability capability NOUN ajst-32233 27 16 of of ADP ajst-32233 27 17 the the DET ajst-32233 27 18 composite composite ADJ ajst-32233 27 19 adjuvant adjuvant NOUN ajst-32233 27 20 were be AUX ajst-32233 27 21 characterized characterize VERB ajst-32233 27 22 . . PUNCT ajst-32233 28 1 2 2 X ajst-32233 28 2 . . X ajst-32233 28 3 experimental experimental ADJ ajst-32233 28 4 2.1 2.1 NUM ajst-32233 28 5 materials material NOUN ajst-32233 28 6 dodecyl dodecyl NOUN ajst-32233 28 7 acrylate acrylate NOUN ajst-32233 28 8 ( ( PUNCT ajst-32233 28 9 la la NOUN ajst-32233 28 10 ) ) PUNCT ajst-32233 28 11 was be AUX ajst-32233 28 12 purchased purchase VERB ajst-32233 28 13 from from ADP ajst-32233 28 14 ryoji ryoji NOUN ajst-32233 28 15 chemical chemical NOUN ajst-32233 28 16 . . PUNCT ajst-32233 29 1 acrylic acrylic PROPN ajst-32233 29 2 acid acid PROPN ajst-32233 29 3 was be AUX ajst-32233 29 4 purchased purchase VERB ajst-32233 29 5 from from ADP ajst-32233 29 6 tianjin tianjin PROPN ajst-32233 29 7 damao damao PROPN ajst-32233 29 8 chemical chemical PROPN ajst-32233 29 9 reagent reagent ADJ ajst-32233 29 10 factory factory NOUN ajst-32233 29 11 . . PUNCT ajst-32233 30 1 polyi polyi PROPN ajst-32233 30 2 : : PUNCT ajst-32233 30 3 c c PROPN ajst-32233 30 4 was be AUX ajst-32233 30 5 purchased purchase VERB ajst-32233 30 6 from from ADP ajst-32233 30 7 kaipeng kaipeng PROPN ajst-32233 30 8 biotechnology biotechnology PROPN ajst-32233 30 9 co. co. PROPN ajst-32233 30 10 , , PUNCT ajst-32233 30 11 ltd ltd PROPN ajst-32233 30 12 . . PROPN ajst-32233 30 13 azobisisobutyronitrile azobisisobutyronitrile PROPN ajst-32233 30 14 and and CCONJ ajst-32233 30 15 allyl allyl ADJ ajst-32233 30 16 pentaerythritol pentaerythritol NOUN ajst-32233 30 17 ( ( PUNCT ajst-32233 30 18 ape ape NOUN ajst-32233 30 19 ) ) PUNCT ajst-32233 30 20 were be AUX ajst-32233 30 21 purchased purchase VERB ajst-32233 30 22 from from ADP ajst-32233 30 23 shanghai shanghai PROPN ajst-32233 31 1 aladdin aladdin VERB ajst-32233 31 2 biochemical biochemical ADJ ajst-32233 31 3 technology technology PROPN ajst-32233 31 4 co. co. PROPN ajst-32233 31 5 , , PUNCT ajst-32233 31 6 ltd ltd PROPN ajst-32233 31 7 . . PROPN ajst-32233 31 8 cyclohexane cyclohexane NOUN ajst-32233 31 9 was be AUX ajst-32233 31 10 purchased purchase VERB ajst-32233 31 11 from from ADP ajst-32233 31 12 shanghai shanghai PROPN ajst-32233 31 13 maclin maclin PROPN ajst-32233 31 14 biochemical biochemical PROPN ajst-32233 31 15 technology technology PROPN ajst-32233 31 16 co. co. PROPN ajst-32233 31 17 , , PUNCT ajst-32233 31 18 ltd ltd PROPN ajst-32233 31 19 . . PROPN ajst-32233 31 20 ethyl ethyl PROPN ajst-32233 31 21 acetate acetate PROPN ajst-32233 31 22 was be AUX ajst-32233 31 23 purchased purchase VERB ajst-32233 31 24 from from ADP ajst-32233 31 25 tianjin tianjin PROPN ajst-32233 31 26 city city PROPN ajst-32233 31 27 yongda yongda PROPN ajst-32233 31 28 chemical chemical PROPN ajst-32233 31 29 reagent reagent PROPN ajst-32233 31 30 co. co. PROPN ajst-32233 31 31 , , PUNCT ajst-32233 31 32 ltd ltd PROPN ajst-32233 31 33 . . PROPN ajst-32233 31 34 nanoemulsion nanoemulsion NOUN ajst-32233 31 35 was be AUX ajst-32233 31 36 self self NOUN ajst-32233 31 37 - - PUNCT ajst-32233 31 38 made make VERB ajst-32233 31 39 by by ADP ajst-32233 31 40 our our PRON ajst-32233 31 41 research research NOUN ajst-32233 31 42 group group NOUN ajst-32233 31 43 . . PUNCT ajst-32233 32 1 2.2 2.2 NUM ajst-32233 32 2 preparation preparation NOUN ajst-32233 32 3 of of ADP ajst-32233 32 4 acrylate acrylate ADJ ajst-32233 32 5 hydrogel hydrogel NOUN ajst-32233 32 6 complex complex NOUN ajst-32233 32 7 adjuvant adjuvant ADJ ajst-32233 32 8 aa aa PROPN ajst-32233 32 9 , , PUNCT ajst-32233 32 10 nahco3 nahco3 ADJ ajst-32233 32 11 , , PUNCT ajst-32233 32 12 dodecyl dodecyl ADJ ajst-32233 32 13 acrylate acrylate NOUN ajst-32233 32 14 , , PUNCT ajst-32233 32 15 ape ape NOUN ajst-32233 32 16 , , PUNCT ajst-32233 32 17 aibn aibn PROPN ajst-32233 32 18 , , PUNCT ajst-32233 32 19 dispersant dispersant NOUN ajst-32233 32 20 , , PUNCT ajst-32233 32 21 cyclohexane cyclohexane NOUN ajst-32233 32 22 and and CCONJ ajst-32233 32 23 ethyl ethyl PROPN ajst-32233 32 24 acetate acetate PROPN ajst-32233 32 25 were be AUX ajst-32233 32 26 added add VERB ajst-32233 32 27 into into ADP ajst-32233 32 28 a a DET ajst-32233 32 29 four four NUM ajst-32233 32 30 - - PUNCT ajst-32233 32 31 necked neck VERB ajst-32233 32 32 flask flask NOUN ajst-32233 32 33 , , PUNCT ajst-32233 32 34 mechanically mechanically ADV ajst-32233 32 35 stirred stir VERB ajst-32233 32 36 for for ADP ajst-32233 32 37 20 20 NUM ajst-32233 32 38 min min NOUN ajst-32233 32 39 under under ADP ajst-32233 32 40 n2 n2 ADJ ajst-32233 32 41 atmosphere atmosphere NOUN ajst-32233 32 42 , , PUNCT ajst-32233 32 43 then then ADV ajst-32233 32 44 the the DET ajst-32233 32 45 temperature temperature NOUN ajst-32233 32 46 of of ADP ajst-32233 32 47 the the DET ajst-32233 32 48 system system NOUN ajst-32233 32 49 was be AUX ajst-32233 32 50 raised raise VERB ajst-32233 32 51 to to ADP ajst-32233 32 52 68 68 NUM ajst-32233 32 53 ℃ ℃ PROPN ajst-32233 32 54 , , PUNCT ajst-32233 32 55 white white ADJ ajst-32233 32 56 particles particle NOUN ajst-32233 32 57 appeared appear VERB ajst-32233 32 58 after after ADP ajst-32233 32 59 a a DET ajst-32233 32 60 period period NOUN ajst-32233 32 61 of of ADP ajst-32233 32 62 time time NOUN ajst-32233 32 63 , , PUNCT ajst-32233 32 64 reacted react VERB ajst-32233 32 65 at at ADP ajst-32233 32 66 constant constant ADJ ajst-32233 32 67 temperature temperature NOUN ajst-32233 32 68 for for ADP ajst-32233 32 69 4h 4h NUM ajst-32233 32 70 , , PUNCT ajst-32233 32 71 stopped stop VERB ajst-32233 32 72 heating heating NOUN ajst-32233 32 73 , , PUNCT ajst-32233 32 74 filtered filter VERB ajst-32233 32 75 and and CCONJ ajst-32233 32 76 washed wash VERB ajst-32233 32 77 with with ADP ajst-32233 32 78 ethyl ethyl PROPN ajst-32233 32 79 acetate acetate NOUN ajst-32233 32 80 when when SCONJ ajst-32233 32 81 the the DET ajst-32233 32 82 temperature temperature NOUN ajst-32233 32 83 dropped drop VERB ajst-32233 32 84 below below ADP ajst-32233 32 85 30 30 NUM ajst-32233 32 86 ℃ ℃ PROPN ajst-32233 32 87 . . PUNCT ajst-32233 33 1 the the DET ajst-32233 33 2 white white ADJ ajst-32233 33 3 product product NOUN ajst-32233 33 4 was be AUX ajst-32233 33 5 dried dry VERB ajst-32233 33 6 at at ADP ajst-32233 33 7 55 55 NUM ajst-32233 33 8 ℃ ℃ PROPN ajst-32233 33 9 for for ADP ajst-32233 33 10 48 48 NUM ajst-32233 33 11 h h NOUN ajst-32233 33 12 in in ADP ajst-32233 33 13 a a DET ajst-32233 33 14 vacuum vacuum NOUN ajst-32233 33 15 oven oven NOUN ajst-32233 33 16 , , PUNCT ajst-32233 33 17 then then ADV ajst-32233 33 18 swelled swell VERB ajst-32233 33 19 in in ADP ajst-32233 33 20 pure pure ADJ ajst-32233 33 21 water water NOUN ajst-32233 33 22 , , PUNCT ajst-32233 33 23 and and CCONJ ajst-32233 33 24 then then ADV ajst-32233 33 25 adjusted adjust VERB ajst-32233 33 26 to to ADP ajst-32233 33 27 ph ph PROPN ajst-32233 33 28 7 7 NUM ajst-32233 33 29 with with ADP ajst-32233 33 30 naoh naoh ADJ ajst-32233 33 31 solution solution NOUN ajst-32233 33 32 to to PART ajst-32233 33 33 form form VERB ajst-32233 33 34 acrylate acrylate NOUN ajst-32233 33 35 hydrogel hydrogel NOUN ajst-32233 33 36 . . PUNCT ajst-32233 34 1 polyacrylate polyacrylate ADJ ajst-32233 34 2 nanoemulsion nanoemulsion NOUN ajst-32233 34 3 ( ( PUNCT ajst-32233 34 4 pnoe pnoe NOUN ajst-32233 34 5 ) ) PUNCT ajst-32233 34 6 was be AUX ajst-32233 34 7 prepared prepare VERB ajst-32233 34 8 by by ADP ajst-32233 34 9 mixing mix VERB ajst-32233 34 10 acrylate acrylate NOUN ajst-32233 34 11 hydrogel hydrogel NOUN ajst-32233 34 12 with with ADP ajst-32233 34 13 self self NOUN ajst-32233 34 14 - - PUNCT ajst-32233 34 15 made make VERB ajst-32233 34 16 nanoemulsion nanoemulsion NOUN ajst-32233 34 17 . . PUNCT ajst-32233 35 1 finally finally ADV ajst-32233 35 2 , , PUNCT ajst-32233 35 3 acrylate acrylate NOUN ajst-32233 35 4 hydrogel hydrogel NOUN ajst-32233 35 5 complex complex NOUN ajst-32233 35 6 adjuvant adjuvant ADJ ajst-32233 35 7 ( ( PUNCT ajst-32233 35 8 ahcp ahcp PROPN ajst-32233 35 9 ) ) PUNCT ajst-32233 35 10 was be AUX ajst-32233 35 11 prepared prepare VERB ajst-32233 35 12 by by ADP ajst-32233 35 13 mixing mix VERB ajst-32233 35 14 pnoe pnoe NOUN ajst-32233 35 15 with with ADP ajst-32233 35 16 a a DET ajst-32233 35 17 certain certain ADJ ajst-32233 35 18 amount amount NOUN ajst-32233 35 19 of of ADP ajst-32233 35 20 10 10 NUM ajst-32233 35 21 mg mg NUM ajst-32233 35 22 / / SYM ajst-32233 35 23 ml ml X ajst-32233 35 24 poly poly ADJ ajst-32233 36 1 i i PRON ajst-32233 36 2 : : PUNCT ajst-32233 36 3 c c NOUN ajst-32233 36 4 solution solution NOUN ajst-32233 36 5 . . PUNCT ajst-32233 37 1 2.3 2.3 NUM ajst-32233 37 2 morphology morphology NOUN ajst-32233 37 3 of of ADP ajst-32233 37 4 ahcp ahcp PROPN ajst-32233 37 5 transmission transmission NOUN ajst-32233 37 6 electron electron NOUN ajst-32233 37 7 microscope microscope NOUN ajst-32233 37 8 ( ( PUNCT ajst-32233 37 9 tem tem PROPN ajst-32233 37 10 , , PUNCT ajst-32233 37 11 jem-2100plus jem-2100plus NUM ajst-32233 37 12 , , PUNCT ajst-32233 37 13 nippon nippon PROPN ajst-32233 37 14 electronics electronic NOUN ajst-32233 37 15 ) ) PUNCT ajst-32233 37 16 was be AUX ajst-32233 37 17 used use VERB ajst-32233 37 18 to to PART ajst-32233 37 19 observe observe VERB ajst-32233 37 20 the the DET ajst-32233 37 21 micro micro NOUN ajst-32233 37 22 - - NOUN ajst-32233 37 23 morphology morphology NOUN ajst-32233 37 24 of of ADP ajst-32233 37 25 the the DET ajst-32233 37 26 ahcp ahcp NOUN ajst-32233 37 27 . . PUNCT ajst-32233 38 1 2.4 2.4 NUM ajst-32233 38 2 particle particle NOUN ajst-32233 38 3 size size NOUN ajst-32233 38 4 and and CCONJ ajst-32233 38 5 zeta zeta NOUN ajst-32233 38 6 potential potential NOUN ajst-32233 38 7 the the DET ajst-32233 38 8 particle particle NOUN ajst-32233 38 9 size size NOUN ajst-32233 38 10 and and CCONJ ajst-32233 38 11 zeta zeta NOUN ajst-32233 38 12 potential potential NOUN ajst-32233 38 13 of of ADP ajst-32233 38 14 acrylate acrylate ADJ ajst-32233 38 15 hydrogel hydrogel NOUN ajst-32233 38 16 complex complex NOUN ajst-32233 38 17 adjuvant adjuvant NOUN ajst-32233 38 18 were be AUX ajst-32233 38 19 measured measure VERB ajst-32233 38 20 by by ADP ajst-32233 38 21 dynamic dynamic ADJ ajst-32233 38 22 light light NOUN ajst-32233 38 23 scattering scatter VERB ajst-32233 38 24 particle particle NOUN ajst-32233 38 25 size size NOUN ajst-32233 38 26 analyzer analyzer NOUN ajst-32233 38 27 ( ( PUNCT ajst-32233 38 28 z3000 z3000 PROPN ajst-32233 38 29 , , PUNCT ajst-32233 38 30 pss pss PROPN ajst-32233 38 31 , , PUNCT ajst-32233 38 32 usa usa PROPN ajst-32233 38 33 ) ) PUNCT ajst-32233 38 34 . . PUNCT ajst-32233 39 1 take take VERB ajst-32233 39 2 the the DET ajst-32233 39 3 ahcp ahcp NOUN ajst-32233 39 4 and and CCONJ ajst-32233 39 5 dilute dilute VERB ajst-32233 39 6 it it PRON ajst-32233 39 7 with with ADP ajst-32233 39 8 pure pure ADJ ajst-32233 39 9 water water NOUN ajst-32233 39 10 to to ADP ajst-32233 39 11 15 15 NUM ajst-32233 39 12 wt% wt% ADJ ajst-32233 39 13 as as SCONJ ajst-32233 39 14 the the DET ajst-32233 39 15 particle particle NOUN ajst-32233 39 16 size size NOUN ajst-32233 39 17 sample sample NOUN ajst-32233 39 18 to to PART ajst-32233 39 19 be be AUX ajst-32233 39 20 tested test VERB ajst-32233 39 21 , , PUNCT ajst-32233 39 22 and and CCONJ ajst-32233 39 23 dilute dilute VERB ajst-32233 39 24 it it PRON ajst-32233 39 25 to to ADP ajst-32233 39 26 2 2 NUM ajst-32233 39 27 wt% wt% ADJ ajst-32233 39 28 as as SCONJ ajst-32233 39 29 the the DET ajst-32233 39 30 zeta zeta PROPN ajst-32233 39 31 potential potential ADJ ajst-32233 39 32 sample sample NOUN ajst-32233 39 33 to to PART ajst-32233 39 34 be be AUX ajst-32233 39 35 tested test VERB ajst-32233 39 36 . . PUNCT ajst-32233 40 1 particle particle NOUN ajst-32233 40 2 size size NOUN ajst-32233 40 3 test test NOUN ajst-32233 40 4 conditions condition NOUN ajst-32233 40 5 : : PUNCT ajst-32233 40 6 test test NOUN ajst-32233 40 7 temperature temperature NOUN ajst-32233 40 8 is be AUX ajst-32233 40 9 23 23 NUM ajst-32233 40 10 ℃ ℃ PROPN ajst-32233 40 11 , , PUNCT ajst-32233 40 12 detection detection NOUN ajst-32233 40 13 angle angle NOUN ajst-32233 40 14 is be AUX ajst-32233 40 15 90 90 NUM ajst-32233 40 16 ° ° NOUN ajst-32233 40 17 , , PUNCT ajst-32233 40 18 each each DET ajst-32233 40 19 sample sample NOUN ajst-32233 40 20 is be AUX ajst-32233 40 21 tested test VERB ajst-32233 40 22 3 3 NUM ajst-32233 40 23 times time NOUN ajst-32233 40 24 in in ADP ajst-32233 40 25 parallel parallel NOUN ajst-32233 40 26 . . PUNCT ajst-32233 41 1 zeta zeta PROPN ajst-32233 41 2 potential potential ADJ ajst-32233 41 3 test test NOUN ajst-32233 41 4 conditions condition NOUN ajst-32233 41 5 : : PUNCT ajst-32233 41 6 the the DET ajst-32233 41 7 test test NOUN ajst-32233 41 8 temperature temperature NOUN ajst-32233 41 9 is be AUX ajst-32233 41 10 23 23 NUM ajst-32233 41 11 ℃ ℃ PROPN ajst-32233 41 12 , , PUNCT ajst-32233 41 13 the the DET ajst-32233 41 14 temperature temperature NOUN ajst-32233 41 15 of of ADP ajst-32233 41 16 the the DET ajst-32233 41 17 detection detection NOUN ajst-32233 41 18 cell cell NOUN ajst-32233 41 19 is be AUX ajst-32233 41 20 balanced balance VERB ajst-32233 41 21 for for ADP ajst-32233 41 22 60s 60 NOUN ajst-32233 41 23 before before ADP ajst-32233 41 24 detection detection NOUN ajst-32233 41 25 , , PUNCT ajst-32233 41 26 and and CCONJ ajst-32233 41 27 each each DET ajst-32233 41 28 sample sample NOUN ajst-32233 41 29 is be AUX ajst-32233 41 30 measured measure VERB ajst-32233 41 31 for for ADP ajst-32233 41 32 3 3 NUM ajst-32233 41 33 times time NOUN ajst-32233 41 34 . . PUNCT ajst-32233 42 1 2.5 2.5 NUM ajst-32233 42 2 rotational rotational ADJ ajst-32233 42 3 viscosity viscosity NOUN ajst-32233 42 4 flow flow NOUN ajst-32233 42 5 scanning scanning NOUN ajst-32233 42 6 and and CCONJ ajst-32233 42 7 oscillation oscillation NOUN ajst-32233 42 8 amplitude amplitude NOUN ajst-32233 42 9 testing testing NOUN ajst-32233 42 10 of of ADP ajst-32233 42 11 ahcp ahcp NOUN ajst-32233 42 12 were be AUX ajst-32233 42 13 performed perform VERB ajst-32233 42 14 using use VERB ajst-32233 42 15 a a DET ajst-32233 42 16 rotational rotational ADJ ajst-32233 42 17 rheometer rheometer NOUN ajst-32233 42 18 ( ( PUNCT ajst-32233 42 19 dhr-1 dhr-1 NOUN ajst-32233 42 20 , , PUNCT ajst-32233 42 21 ta ta PROPN ajst-32233 42 22 inc inc PROPN ajst-32233 42 23 . . PROPN ajst-32233 42 24 , , PUNCT ajst-32233 42 25 usa usa PROPN ajst-32233 42 26 ) ) PUNCT ajst-32233 42 27 . . PUNCT ajst-32233 43 1 flow flow NOUN ajst-32233 43 2 scanning scan VERB ajst-32233 43 3 test test NOUN ajst-32233 43 4 conditions condition NOUN ajst-32233 43 5 : : PUNCT ajst-32233 43 6 25 25 NUM ajst-32233 43 7 mm mm NOUN ajst-32233 43 8 plate plate NOUN ajst-32233 43 9 , , PUNCT ajst-32233 43 10 shear shear NOUN ajst-32233 43 11 rate rate NOUN ajst-32233 43 12 : : PUNCT ajst-32233 43 13 1~100 1~100 NUM ajst-32233 43 14 s3 s3 PROPN ajst-32233 43 15 1 1 NUM ajst-32233 43 16 , , PUNCT ajst-32233 43 17 test test NOUN ajst-32233 43 18 temperature temperature NOUN ajst-32233 43 19 : : PUNCT ajst-32233 43 20 25 25 NUM ajst-32233 43 21 ℃ ℃ PROPN ajst-32233 43 22 . . PUNCT ajst-32233 43 23 oscillation oscillation NOUN ajst-32233 43 24 amplitude amplitude NOUN ajst-32233 43 25 test test NOUN ajst-32233 43 26 conditions condition NOUN ajst-32233 43 27 : : PUNCT ajst-32233 43 28 frequency frequency NOUN ajst-32233 43 29 : : PUNCT ajst-32233 43 30 10 10 NUM ajst-32233 43 31 rad rad PROPN ajst-32233 43 32 / / SYM ajst-32233 43 33 s s PROPN ajst-32233 43 34 , , PUNCT ajst-32233 43 35 strain strain NOUN ajst-32233 43 36 : : PUNCT ajst-32233 43 37 1~1000 1~1000 NUM ajst-32233 43 38 % % NOUN ajst-32233 43 39 , , PUNCT ajst-32233 43 40 test test NOUN ajst-32233 43 41 temperature temperature NOUN ajst-32233 43 42 : : PUNCT ajst-32233 43 43 25 25 NUM ajst-32233 43 44 ℃ ℃ PROPN ajst-32233 43 45 . . NOUN ajst-32233 43 46 2.6 2.6 NUM ajst-32233 43 47 cytotoxicity cytotoxicity NOUN ajst-32233 43 48 the the DET ajst-32233 43 49 macrophages macrophage NOUN ajst-32233 43 50 were be AUX ajst-32233 43 51 adjusted adjust VERB ajst-32233 43 52 to to ADP ajst-32233 43 53 a a DET ajst-32233 43 54 concentration concentration NOUN ajst-32233 43 55 of of ADP ajst-32233 43 56 1×105 1×105 NUM ajst-32233 43 57 cells cell NOUN ajst-32233 43 58 / / SYM ajst-32233 43 59 ml ml ADV ajst-32233 43 60 and and CCONJ ajst-32233 43 61 seeded seed VERB ajst-32233 43 62 into into ADP ajst-32233 43 63 a a DET ajst-32233 43 64 cell cell NOUN ajst-32233 43 65 culture culture NOUN ajst-32233 43 66 plate plate NOUN ajst-32233 43 67 at at ADP ajst-32233 43 68 100 100 NUM ajst-32233 43 69 μl μl ADP ajst-32233 43 70 per per ADV ajst-32233 43 71 well well ADV ajst-32233 43 72 . . PUNCT ajst-32233 44 1 the the DET ajst-32233 44 2 plate plate NOUN ajst-32233 44 3 was be AUX ajst-32233 44 4 placed place VERB ajst-32233 44 5 in in ADP ajst-32233 44 6 a a DET ajst-32233 44 7 co2 co2 NOUN ajst-32233 44 8 incubator incubator NOUN ajst-32233 44 9 to to PART ajst-32233 44 10 allow allow VERB ajst-32233 44 11 cell cell NOUN ajst-32233 44 12 adhesion adhesion NOUN ajst-32233 44 13 . . PUNCT ajst-32233 45 1 pnoe pnoe PROPN ajst-32233 45 2 and and CCONJ ajst-32233 45 3 ahcp ahcp NOUN ajst-32233 45 4 were be AUX ajst-32233 45 5 added add VERB ajst-32233 45 6 to to ADP ajst-32233 45 7 the the DET ajst-32233 45 8 cell cell NOUN ajst-32233 45 9 culture culture NOUN ajst-32233 45 10 plate plate NOUN ajst-32233 45 11 at at ADP ajst-32233 45 12 concentrations concentration NOUN ajst-32233 45 13 of of ADP ajst-32233 45 14 2 2 NUM ajst-32233 45 15 mg mg NUM ajst-32233 45 16 / / SYM ajst-32233 45 17 ml ml NOUN ajst-32233 45 18 and and CCONJ ajst-32233 45 19 1 1 NUM ajst-32233 45 20 mg mg NUM ajst-32233 45 21 / / SYM ajst-32233 45 22 ml ml NOUN ajst-32233 45 23 , , PUNCT ajst-32233 45 24 respectively respectively ADV ajst-32233 45 25 , , PUNCT ajst-32233 45 26 while while SCONJ ajst-32233 45 27 poly poly ADJ ajst-32233 45 28 i i PRON ajst-32233 45 29 : : PUNCT ajst-32233 45 30 c c PROPN ajst-32233 45 31 was be AUX ajst-32233 45 32 added add VERB ajst-32233 45 33 at at ADP ajst-32233 45 34 concentrations concentration NOUN ajst-32233 45 35 of of ADP ajst-32233 45 36 20 20 NUM ajst-32233 45 37 μg μg NOUN ajst-32233 45 38 / / SYM ajst-32233 45 39 ml ml PROPN ajst-32233 45 40 and and CCONJ ajst-32233 45 41 10 10 NUM ajst-32233 45 42 μg μg NOUN ajst-32233 45 43 / / SYM ajst-32233 45 44 ml ml NOUN ajst-32233 45 45 . . PUNCT ajst-32233 46 1 each each DET ajst-32233 46 2 drug drug NOUN ajst-32233 46 3 concentration concentration NOUN ajst-32233 46 4 was be AUX ajst-32233 46 5 tested test VERB ajst-32233 46 6 in in ADP ajst-32233 46 7 three three NUM ajst-32233 46 8 times time NOUN ajst-32233 46 9 , , PUNCT ajst-32233 46 10 with with ADP ajst-32233 46 11 control control NOUN ajst-32233 46 12 wells well NOUN ajst-32233 46 13 ( ( PUNCT ajst-32233 46 14 cell cell NOUN ajst-32233 46 15 control control NOUN ajst-32233 46 16 ) ) PUNCT ajst-32233 46 17 and and CCONJ ajst-32233 46 18 blank blank ADJ ajst-32233 46 19 wells well NOUN ajst-32233 46 20 set set VERB ajst-32233 46 21 up up ADP ajst-32233 46 22 simultaneously simultaneously ADV ajst-32233 46 23 . . PUNCT ajst-32233 47 1 after after ADP ajst-32233 47 2 12 12 NUM ajst-32233 47 3 hours hour NOUN ajst-32233 47 4 of of ADP ajst-32233 47 5 incubation incubation NOUN ajst-32233 47 6 , , PUNCT ajst-32233 47 7 the the DET ajst-32233 47 8 cytotoxicity cytotoxicity NOUN ajst-32233 47 9 of of ADP ajst-32233 47 10 the the DET ajst-32233 47 11 drugs drug NOUN ajst-32233 47 12 on on ADP ajst-32233 47 13 macrophages macrophage NOUN ajst-32233 47 14 was be AUX ajst-32233 47 15 assessed assess VERB ajst-32233 47 16 using use VERB ajst-32233 47 17 the the DET ajst-32233 47 18 cck-8 cck-8 PUNCT ajst-32233 47 19 method method NOUN ajst-32233 47 20 . . PUNCT ajst-32233 48 1 the the DET ajst-32233 48 2 absorbance absorbance NOUN ajst-32233 48 3 at at ADP ajst-32233 48 4 450 450 NUM ajst-32233 48 5 nm nm NOUN ajst-32233 48 6 was be AUX ajst-32233 48 7 measured measure VERB ajst-32233 48 8 with with ADP ajst-32233 48 9 a a DET ajst-32233 48 10 microplate microplate NOUN ajst-32233 48 11 reader reader NOUN ajst-32233 48 12 , , PUNCT ajst-32233 48 13 and and CCONJ ajst-32233 48 14 the the DET ajst-32233 48 15 cell cell NOUN ajst-32233 48 16 viability viability NOUN ajst-32233 48 17 rate rate NOUN ajst-32233 48 18 was be AUX ajst-32233 48 19 calculated calculate VERB ajst-32233 48 20 . . PUNCT ajst-32233 49 1 cell cell NOUN ajst-32233 49 2 viability= viability= VERB ajst-32233 49 3 as as ADP ajst-32233 49 4 - - PUNCT ajst-32233 49 5 ab ab PRON ajst-32233 49 6 ac ac PROPN ajst-32233 49 7 - - PROPN ajst-32233 49 8 ab ab PROPN ajst-32233 49 9 ×100 ×100 PROPN ajst-32233 49 10 % % NOUN ajst-32233 49 11 ( ( PUNCT ajst-32233 49 12 1 1 NUM ajst-32233 49 13 ) ) PUNCT ajst-32233 49 14 as as ADP ajst-32233 49 15 : : PUNCT ajst-32233 49 16 absorbance absorbance NOUN ajst-32233 49 17 of of ADP ajst-32233 49 18 experimental experimental ADJ ajst-32233 49 19 wells well NOUN ajst-32233 49 20 . . PUNCT ajst-32233 50 1 ac ac PROPN ajst-32233 50 2 : : PUNCT ajst-32233 50 3 absorbance absorbance NOUN ajst-32233 50 4 of of ADP ajst-32233 50 5 control control NOUN ajst-32233 50 6 well well ADV ajst-32233 50 7 . . PUNCT ajst-32233 51 1 ab ab X ajst-32233 51 2 : : PUNCT ajst-32233 51 3 absorbance absorbance NOUN ajst-32233 51 4 of of ADP ajst-32233 51 5 blank blank ADJ ajst-32233 51 6 well well ADV ajst-32233 51 7 . . PUNCT ajst-32233 52 1 2.7 2.7 NUM ajst-32233 52 2 phagocytosis phagocytosis NOUN ajst-32233 52 3 macrophages macrophage NOUN ajst-32233 52 4 were be AUX ajst-32233 52 5 adjusted adjust VERB ajst-32233 52 6 to to ADP ajst-32233 52 7 a a DET ajst-32233 52 8 concentration concentration NOUN ajst-32233 52 9 of of ADP ajst-32233 52 10 4×10⁵ 4×10⁵ NUM ajst-32233 52 11 cells cell NOUN ajst-32233 52 12 / / SYM ajst-32233 52 13 ml ml NOUN ajst-32233 52 14 , , PUNCT ajst-32233 52 15 and and CCONJ ajst-32233 52 16 the the DET ajst-32233 52 17 macrophage macrophage ADJ ajst-32233 52 18 suspension suspension NOUN ajst-32233 52 19 was be AUX ajst-32233 52 20 added add VERB ajst-32233 52 21 to to ADP ajst-32233 52 22 the the DET ajst-32233 52 23 cell cell NOUN ajst-32233 52 24 culture culture NOUN ajst-32233 52 25 plate plate NOUN ajst-32233 52 26 at at ADP ajst-32233 52 27 1 1 NUM ajst-32233 52 28 ml ml NOUN ajst-32233 52 29 / / SYM ajst-32233 52 30 well well NOUN ajst-32233 52 31 to to PART ajst-32233 52 32 allow allow VERB ajst-32233 52 33 cell cell NOUN ajst-32233 52 34 adhesion adhesion NOUN ajst-32233 52 35 . . PUNCT ajst-32233 53 1 pnoe pnoe PROPN ajst-32233 53 2 and and CCONJ ajst-32233 53 3 ahcp ahcp NOUN ajst-32233 53 4 were be AUX ajst-32233 53 5 complexed complexe VERB ajst-32233 53 6 with with ADP ajst-32233 53 7 fitc fitc NOUN ajst-32233 53 8 - - PUNCT ajst-32233 53 9 ova ova NOUN ajst-32233 53 10 respectively respectively ADV ajst-32233 53 11 and and CCONJ ajst-32233 53 12 then then ADV ajst-32233 53 13 added add VERB ajst-32233 53 14 to to ADP ajst-32233 53 15 the the DET ajst-32233 53 16 cell cell NOUN ajst-32233 53 17 culture culture NOUN ajst-32233 53 18 plate plate NOUN ajst-32233 53 19 . . PUNCT ajst-32233 54 1 blank blank ADJ ajst-32233 54 2 control control NOUN ajst-32233 54 3 and and CCONJ ajst-32233 54 4 fitc fitc NOUN ajst-32233 54 5 - - PUNCT ajst-32233 54 6 ova ova ADJ ajst-32233 54 7 control control NOUN ajst-32233 54 8 groups group NOUN ajst-32233 54 9 were be AUX ajst-32233 54 10 also also ADV ajst-32233 54 11 set set VERB ajst-32233 54 12 up up ADP ajst-32233 54 13 . . PUNCT ajst-32233 55 1 the the DET ajst-32233 55 2 plate plate NOUN ajst-32233 55 3 was be AUX ajst-32233 55 4 incubated incubate VERB ajst-32233 55 5 in in ADP ajst-32233 55 6 a a DET ajst-32233 55 7 co₂ co₂ NOUN ajst-32233 55 8 incubator incubator NOUN ajst-32233 55 9 for for ADP ajst-32233 55 10 12 12 NUM ajst-32233 55 11 hours hour NOUN ajst-32233 55 12 . . PUNCT ajst-32233 56 1 after after ADP ajst-32233 56 2 incubation incubation NOUN ajst-32233 56 3 , , PUNCT ajst-32233 56 4 the the DET ajst-32233 56 5 excess excess ADJ ajst-32233 56 6 liquid liquid NOUN ajst-32233 56 7 was be AUX ajst-32233 56 8 aspirated aspirate VERB ajst-32233 56 9 and and CCONJ ajst-32233 56 10 discarded discard VERB ajst-32233 56 11 . . PUNCT ajst-32233 57 1 each each DET ajst-32233 57 2 well well INTJ ajst-32233 57 3 was be AUX ajst-32233 57 4 washed wash VERB ajst-32233 57 5 twice twice ADV ajst-32233 57 6 with with ADP ajst-32233 57 7 pbs pbs PROPN ajst-32233 57 8 , , PUNCT ajst-32233 57 9 followed follow VERB ajst-32233 57 10 by by ADP ajst-32233 57 11 fixation fixation NOUN ajst-32233 57 12 with with ADP ajst-32233 57 13 4 4 NUM ajst-32233 57 14 % % NOUN ajst-32233 57 15 paraformaldehyde paraformaldehyde NOUN ajst-32233 57 16 fixative fixative NOUN ajst-32233 57 17 for for ADP ajst-32233 57 18 20 20 NUM ajst-32233 57 19 minutes minute NOUN ajst-32233 57 20 . . PUNCT ajst-32233 58 1 the the DET ajst-32233 58 2 wells well NOUN ajst-32233 58 3 were be AUX ajst-32233 58 4 then then ADV ajst-32233 58 5 washed wash VERB ajst-32233 58 6 three three NUM ajst-32233 58 7 times time NOUN ajst-32233 58 8 with with ADP ajst-32233 58 9 pbs pbs PROPN ajst-32233 58 10 . . PUNCT ajst-32233 59 1 dapi dapi ADJ ajst-32233 59 2 staining stain VERB ajst-32233 59 3 solution solution NOUN ajst-32233 59 4 was be AUX ajst-32233 59 5 added add VERB ajst-32233 59 6 , , PUNCT ajst-32233 59 7 and and CCONJ ajst-32233 59 8 the the DET ajst-32233 59 9 plate plate NOUN ajst-32233 59 10 was be AUX ajst-32233 59 11 covered cover VERB ajst-32233 59 12 with with ADP ajst-32233 59 13 tin tin NOUN ajst-32233 59 14 foil foil NOUN ajst-32233 59 15 to to PART ajst-32233 59 16 avoid avoid VERB ajst-32233 59 17 light light NOUN ajst-32233 59 18 for for ADP ajst-32233 59 19 5 5 NUM ajst-32233 59 20 minutes minute NOUN ajst-32233 59 21 . . PUNCT ajst-32233 60 1 afterward afterward ADV ajst-32233 60 2 , , PUNCT ajst-32233 60 3 the the DET ajst-32233 60 4 cell cell NOUN ajst-32233 60 5 culture culture NOUN ajst-32233 60 6 plate plate NOUN ajst-32233 60 7 was be AUX ajst-32233 60 8 washed wash VERB ajst-32233 60 9 three three NUM ajst-32233 60 10 times time NOUN ajst-32233 60 11 with with ADP ajst-32233 60 12 pbs pbs PROPN ajst-32233 60 13 . . PUNCT ajst-32233 61 1 finally finally ADV ajst-32233 61 2 , , PUNCT ajst-32233 61 3 an an DET ajst-32233 61 4 antifade antifade NOUN ajst-32233 61 5 mounting mount VERB ajst-32233 61 6 agent agent NOUN ajst-32233 61 7 was be AUX ajst-32233 61 8 added add VERB ajst-32233 61 9 , , PUNCT ajst-32233 61 10 and and CCONJ ajst-32233 61 11 the the DET ajst-32233 61 12 phagocytic phagocytic ADJ ajst-32233 61 13 ability ability NOUN ajst-32233 61 14 of of ADP ajst-32233 61 15 the the DET ajst-32233 61 16 macrophages macrophage NOUN ajst-32233 61 17 was be AUX ajst-32233 61 18 observed observe VERB ajst-32233 61 19 using use VERB ajst-32233 61 20 an an DET ajst-32233 61 21 inverted inverted ADJ ajst-32233 61 22 fluorescence fluorescence NOUN ajst-32233 61 23 microscope microscope NOUN ajst-32233 61 24 . . PUNCT ajst-32233 62 1 the the DET ajst-32233 62 2 concentrations concentration NOUN ajst-32233 62 3 of of ADP ajst-32233 62 4 pnoe pnoe NOUN ajst-32233 62 5 and and CCONJ ajst-32233 62 6 ahcp ahcp NOUN ajst-32233 62 7 in in ADP ajst-32233 62 8 each each DET ajst-32233 62 9 well well NOUN ajst-32233 62 10 were be AUX ajst-32233 62 11 2 2 NUM ajst-32233 62 12 mg mg NUM ajst-32233 62 13 / / SYM ajst-32233 62 14 ml ml NOUN ajst-32233 62 15 , , PUNCT ajst-32233 62 16 poly poly ADJ ajst-32233 63 1 i i PRON ajst-32233 63 2 : : PUNCT ajst-32233 63 3 c c PROPN ajst-32233 63 4 was be AUX ajst-32233 63 5 2 2 NUM ajst-32233 63 6 μg μg NOUN ajst-32233 63 7 / / SYM ajst-32233 63 8 ml ml NOUN ajst-32233 63 9 and and CCONJ ajst-32233 63 10 fitc fitc PROPN ajst-32233 63 11 ova ova X ajst-32233 63 12 was be AUX ajst-32233 63 13 10 10 NUM ajst-32233 63 14 μg μg NOUN ajst-32233 63 15 / / SYM ajst-32233 63 16 ml ml NOUN ajst-32233 63 17 . . NOUN ajst-32233 64 1 3 3 X ajst-32233 64 2 . . NOUN ajst-32233 64 3 results result NOUN ajst-32233 64 4 and and CCONJ ajst-32233 64 5 discussion discussion NOUN ajst-32233 64 6 3.1 3.1 NUM ajst-32233 64 7 morphology morphology NOUN ajst-32233 64 8 dilute dilute VERB ajst-32233 64 9 pnoe pnoe NOUN ajst-32233 64 10 and and CCONJ ajst-32233 64 11 ahcp ahcp NOUN ajst-32233 64 12 to to ADP ajst-32233 64 13 15 15 NUM ajst-32233 64 14 wt% wt% VERB ajst-32233 64 15 and and CCONJ ajst-32233 64 16 add add VERB ajst-32233 64 17 them they PRON ajst-32233 64 18 into into ADP ajst-32233 64 19 the the DET ajst-32233 64 20 test test NOUN ajst-32233 64 21 tube tube NOUN ajst-32233 64 22 to to PART ajst-32233 64 23 observe observe VERB ajst-32233 64 24 the the DET ajst-32233 64 25 appearance appearance NOUN ajst-32233 64 26 , , PUNCT ajst-32233 64 27 as as SCONJ ajst-32233 64 28 shown show VERB ajst-32233 64 29 in in ADP ajst-32233 64 30 figure figure NOUN ajst-32233 64 31 1 1 NUM ajst-32233 64 32 . . PUNCT ajst-32233 65 1 the the DET ajst-32233 65 2 results result NOUN ajst-32233 65 3 show show VERB ajst-32233 65 4 that that SCONJ ajst-32233 65 5 they they PRON ajst-32233 65 6 are be AUX ajst-32233 65 7 uniform uniform ADJ ajst-32233 65 8 and and CCONJ ajst-32233 65 9 transparent transparent ADJ ajst-32233 65 10 to to ADP ajst-32233 65 11 blue blue ADJ ajst-32233 65 12 light light NOUN ajst-32233 65 13 . . PUNCT ajst-32233 66 1 at at ADP ajst-32233 66 2 the the DET ajst-32233 66 3 same same ADJ ajst-32233 66 4 time time NOUN ajst-32233 66 5 , , PUNCT ajst-32233 66 6 dilute dilute VERB ajst-32233 66 7 pnoe pnoe NOUN ajst-32233 66 8 and and CCONJ ajst-32233 66 9 ahcp ahcp NOUN ajst-32233 66 10 to to ADP ajst-32233 66 11 0.1 0.1 NUM ajst-32233 66 12 wt% wt% ADJ ajst-32233 66 13 and and CCONJ ajst-32233 66 14 observe observe VERB ajst-32233 66 15 their their PRON ajst-32233 66 16 microscopic microscopic ADJ ajst-32233 66 17 morphology morphology NOUN ajst-32233 66 18 at at ADP ajst-32233 66 19 magnification magnification NOUN ajst-32233 66 20 of of ADP ajst-32233 66 21 20000 20000 NUM ajst-32233 66 22 ×. ×. PRON ajst-32233 66 23 it it PRON ajst-32233 66 24 is be AUX ajst-32233 66 25 found find VERB ajst-32233 66 26 that that SCONJ ajst-32233 66 27 the the DET ajst-32233 66 28 particle particle NOUN ajst-32233 66 29 size size NOUN ajst-32233 66 30 is be AUX ajst-32233 66 31 mostly mostly ADV ajst-32233 66 32 about about ADV ajst-32233 66 33 50 50 NUM ajst-32233 66 34 nm nm NOUN ajst-32233 66 35 , , PUNCT ajst-32233 66 36 the the DET ajst-32233 66 37 particle particle NOUN ajst-32233 66 38 structure structure NOUN ajst-32233 66 39 is be AUX ajst-32233 66 40 complete complete ADJ ajst-32233 66 41 , , PUNCT ajst-32233 66 42 and and CCONJ ajst-32233 66 43 the the DET ajst-32233 66 44 dispersibility dispersibility NOUN ajst-32233 66 45 is be AUX ajst-32233 66 46 relatively relatively ADV ajst-32233 66 47 good good ADJ ajst-32233 66 48 . . PUNCT ajst-32233 67 1 figure figure NOUN ajst-32233 67 2 1 1 NUM ajst-32233 67 3 . . NOUN ajst-32233 67 4 appearance appearance NOUN ajst-32233 67 5 of of ADP ajst-32233 67 6 pnoe pnoe NOUN ajst-32233 67 7 and and CCONJ ajst-32233 67 8 ahcp ahcp NOUN ajst-32233 67 9 diluted dilute VERB ajst-32233 67 10 to to ADP ajst-32233 67 11 15 15 NUM ajst-32233 67 12 wt% wt% ADJ ajst-32233 67 13 . . PUNCT ajst-32233 68 1 4 4 NUM ajst-32233 68 2 figure figure NOUN ajst-32233 68 3 2 2 NUM ajst-32233 68 4 . . NOUN ajst-32233 68 5 microscopic microscopic ADJ ajst-32233 68 6 morphology morphology NOUN ajst-32233 68 7 of of ADP ajst-32233 68 8 ( ( PUNCT ajst-32233 68 9 a a PRON ajst-32233 68 10 ) ) PUNCT ajst-32233 68 11 pnoe pnoe NOUN ajst-32233 68 12 and and CCONJ ajst-32233 68 13 ( ( PUNCT ajst-32233 68 14 b b NOUN ajst-32233 68 15 ) ) PUNCT ajst-32233 68 16 ahcp ahcp NOUN ajst-32233 68 17 . . PUNCT ajst-32233 69 1 3.2 3.2 NUM ajst-32233 69 2 particle particle NOUN ajst-32233 69 3 size size NOUN ajst-32233 69 4 and and CCONJ ajst-32233 69 5 zeta zeta NOUN ajst-32233 69 6 potential potential ADJ ajst-32233 69 7 nanoparticles nanoparticle NOUN ajst-32233 69 8 have have VERB ajst-32233 69 9 unique unique ADJ ajst-32233 69 10 biological biological ADJ ajst-32233 69 11 activity activity NOUN ajst-32233 69 12 due due ADP ajst-32233 69 13 to to ADP ajst-32233 69 14 their their PRON ajst-32233 69 15 large large ADJ ajst-32233 69 16 specific specific ADJ ajst-32233 69 17 surface surface NOUN ajst-32233 69 18 area area NOUN ajst-32233 69 19 due due ADP ajst-32233 69 20 to to ADP ajst-32233 69 21 their their PRON ajst-32233 69 22 small small ADJ ajst-32233 69 23 size size NOUN ajst-32233 69 24 , , PUNCT ajst-32233 69 25 and and CCONJ ajst-32233 69 26 are be AUX ajst-32233 69 27 efficiently efficiently ADV ajst-32233 69 28 delivered deliver VERB ajst-32233 69 29 to to ADP ajst-32233 69 30 antigen antigen ADJ ajst-32233 69 31 presenting present VERB ajst-32233 69 32 cells cell NOUN ajst-32233 69 33 ( ( PUNCT ajst-32233 69 34 apcs apc NOUN ajst-32233 69 35 ) ) PUNCT ajst-32233 69 36 by by ADP ajst-32233 69 37 coating coat VERB ajst-32233 69 38 antigen antigen ADV ajst-32233 69 39 . . PUNCT ajst-32233 70 1 the the DET ajst-32233 70 2 particle particle NOUN ajst-32233 70 3 size size NOUN ajst-32233 70 4 , , PUNCT ajst-32233 70 5 polydispersity polydispersity NOUN ajst-32233 70 6 index index NOUN ajst-32233 70 7 ( ( PUNCT ajst-32233 70 8 p.i p.i PROPN ajst-32233 70 9 . . PUNCT ajst-32233 70 10 ) ) PUNCT ajst-32233 70 11 and and CCONJ ajst-32233 70 12 zeta zeta NOUN ajst-32233 70 13 potential potential NOUN ajst-32233 70 14 of of ADP ajst-32233 70 15 pnoe pnoe NOUN ajst-32233 70 16 and and CCONJ ajst-32233 70 17 ahcp ahcp NOUN ajst-32233 70 18 were be AUX ajst-32233 70 19 measured measure VERB ajst-32233 70 20 , , PUNCT ajst-32233 70 21 and and CCONJ ajst-32233 70 22 the the DET ajst-32233 70 23 results result NOUN ajst-32233 70 24 are be AUX ajst-32233 70 25 shown show VERB ajst-32233 70 26 in in ADP ajst-32233 70 27 table table NOUN ajst-32233 70 28 1 1 NUM ajst-32233 70 29 and and CCONJ ajst-32233 70 30 figure figure VERB ajst-32233 70 31 3 3 NUM ajst-32233 70 32 . . PUNCT ajst-32233 70 33 table table NOUN ajst-32233 70 34 1 1 NUM ajst-32233 70 35 shows show VERB ajst-32233 70 36 that that SCONJ ajst-32233 70 37 the the DET ajst-32233 70 38 average average ADJ ajst-32233 70 39 particle particle NOUN ajst-32233 70 40 size size NOUN ajst-32233 70 41 of of ADP ajst-32233 70 42 pnoe pnoe PROPN ajst-32233 70 43 and and CCONJ ajst-32233 70 44 ahcp ahcp NOUN ajst-32233 70 45 is be AUX ajst-32233 70 46 46.53 46.53 NUM ajst-32233 70 47 nm nm NOUN ajst-32233 70 48 and and CCONJ ajst-32233 70 49 46.67 46.67 NUM ajst-32233 70 50 nm nm NOUN ajst-32233 70 51 , , PUNCT ajst-32233 70 52 respectively respectively ADV ajst-32233 70 53 . . PUNCT ajst-32233 71 1 and and CCONJ ajst-32233 71 2 the the DET ajst-32233 71 3 average average ADJ ajst-32233 71 4 p.i p.i PROPN ajst-32233 71 5 . . PROPN ajst-32233 71 6 is be AUX ajst-32233 71 7 0.22 0.22 NUM ajst-32233 71 8 and and CCONJ ajst-32233 71 9 0.14 0.14 NUM ajst-32233 71 10 , , PUNCT ajst-32233 71 11 respectively respectively ADV ajst-32233 71 12 . . PUNCT ajst-32233 72 1 as as SCONJ ajst-32233 72 2 shown show VERB ajst-32233 72 3 in in ADP ajst-32233 72 4 figure figure NOUN ajst-32233 72 5 3 3 NUM ajst-32233 72 6 , , PUNCT ajst-32233 72 7 the the DET ajst-32233 72 8 zeta zeta NOUN ajst-32233 72 9 potential potential NOUN ajst-32233 72 10 of of ADP ajst-32233 72 11 pnoe pnoe NOUN ajst-32233 72 12 and and CCONJ ajst-32233 72 13 ahcp ahcp NOUN ajst-32233 72 14 were be AUX ajst-32233 72 15 -37.44 -37.44 PROPN ajst-32233 72 16 mv mv PROPN ajst-32233 72 17 and and CCONJ ajst-32233 72 18 -42.05 -42.05 PROPN ajst-32233 72 19 mv mv PROPN ajst-32233 72 20 , , PUNCT ajst-32233 72 21 respectively respectively ADV ajst-32233 72 22 . . PUNCT ajst-32233 73 1 the the DET ajst-32233 73 2 higher high ADJ ajst-32233 73 3 absolute absolute ADJ ajst-32233 73 4 value value NOUN ajst-32233 73 5 of of ADP ajst-32233 73 6 zeta zeta PROPN ajst-32233 73 7 potential potential NOUN ajst-32233 73 8 indicates indicate VERB ajst-32233 73 9 that that SCONJ ajst-32233 73 10 the the DET ajst-32233 73 11 system system NOUN ajst-32233 73 12 is be AUX ajst-32233 73 13 stable stable ADJ ajst-32233 73 14 and and CCONJ ajst-32233 73 15 difficult difficult ADJ ajst-32233 73 16 to to PART ajst-32233 73 17 settle settle VERB ajst-32233 73 18 . . PUNCT ajst-32233 74 1 table table NOUN ajst-32233 74 2 1 1 NUM ajst-32233 74 3 . . PUNCT ajst-32233 75 1 average average ADJ ajst-32233 75 2 particle particle NOUN ajst-32233 75 3 size size NOUN ajst-32233 75 4 and and CCONJ ajst-32233 75 5 p.i p.i PROPN ajst-32233 75 6 . . PROPN ajst-32233 75 7 of of ADP ajst-32233 75 8 adjuvant adjuvant NOUN ajst-32233 75 9 . . PUNCT ajst-32233 76 1 sample sample NOUN ajst-32233 76 2 mean mean VERB ajst-32233 76 3 particle particle NOUN ajst-32233 76 4 diameter diameter NOUN ajst-32233 76 5 / / SYM ajst-32233 76 6 nm nm NOUN ajst-32233 76 7 sd sd ADP ajst-32233 76 8 p.i p.i PROPN ajst-32233 76 9 . . PROPN ajst-32233 77 1 sd sd ADP ajst-32233 77 2 pnoe pnoe PROPN ajst-32233 77 3 46.53 46.53 NUM ajst-32233 77 4 0.602 0.602 NUM ajst-32233 77 5 0.22 0.22 NUM ajst-32233 77 6 0.06 0.06 NUM ajst-32233 77 7 ahcp ahcp NOUN ajst-32233 77 8 46.67 46.67 NUM ajst-32233 77 9 3.62 3.62 NUM ajst-32233 77 10 0.14 0.14 NUM ajst-32233 77 11 0.01 0.01 NUM ajst-32233 77 12 figure figure NOUN ajst-32233 77 13 3 3 NUM ajst-32233 77 14 . . X ajst-32233 77 15 zeta zeta PROPN ajst-32233 77 16 potential potential NOUN ajst-32233 77 17 of of ADP ajst-32233 77 18 pnoe pnoe PROPN ajst-32233 77 19 and and CCONJ ajst-32233 77 20 ahcp ahcp NOUN ajst-32233 77 21 . . PUNCT ajst-32233 78 1 3.3 3.3 NUM ajst-32233 78 2 rheological rheological ADJ ajst-32233 78 3 property property NOUN ajst-32233 78 4 the the DET ajst-32233 78 5 viscoelasticity viscoelasticity NOUN ajst-32233 78 6 of of ADP ajst-32233 78 7 acrylate acrylate NOUN ajst-32233 78 8 hydrogel hydrogel NOUN ajst-32233 78 9 complex complex NOUN ajst-32233 78 10 adjuvant adjuvant NOUN ajst-32233 78 11 may may AUX ajst-32233 78 12 affect affect VERB ajst-32233 78 13 its its PRON ajst-32233 78 14 antigen antigen ADJ ajst-32233 78 15 release release NOUN ajst-32233 78 16 behavior behavior NOUN ajst-32233 78 17 after after ADP ajst-32233 78 18 entering enter VERB ajst-32233 78 19 the the DET ajst-32233 78 20 body body NOUN ajst-32233 78 21 as as ADP ajst-32233 78 22 vaccine vaccine NOUN ajst-32233 78 23 adjuvant adjuvant ADJ ajst-32233 78 24 . . PUNCT ajst-32233 79 1 the the DET ajst-32233 79 2 dynamic dynamic ADJ ajst-32233 79 3 mechanical mechanical ADJ ajst-32233 79 4 behavior behavior NOUN ajst-32233 79 5 of of ADP ajst-32233 79 6 pnoe pnoe PROPN ajst-32233 79 7 and and CCONJ ajst-32233 79 8 ahcp ahcp NOUN ajst-32233 79 9 was be AUX ajst-32233 79 10 studied study VERB ajst-32233 79 11 , , PUNCT ajst-32233 79 12 and and CCONJ ajst-32233 79 13 the the DET ajst-32233 79 14 rheological rheological ADJ ajst-32233 79 15 test test NOUN ajst-32233 79 16 curves curve NOUN ajst-32233 79 17 are be AUX ajst-32233 79 18 shown show VERB ajst-32233 79 19 in in ADP ajst-32233 79 20 figure figure NOUN ajst-32233 79 21 4 4 NUM ajst-32233 79 22 . . PUNCT ajst-32233 79 23 figure figure NOUN ajst-32233 79 24 4a 4a NOUN ajst-32233 79 25 shows show VERB ajst-32233 79 26 that that SCONJ ajst-32233 79 27 shear shear NOUN ajst-32233 79 28 thinning thin VERB ajst-32233 79 29 effect effect NOUN ajst-32233 79 30 is be AUX ajst-32233 79 31 observed observe VERB ajst-32233 79 32 with with ADP ajst-32233 79 33 increasing increase VERB ajst-32233 79 34 shear shear NOUN ajst-32233 79 35 rate rate NOUN ajst-32233 79 36 of of ADP ajst-32233 79 37 the the DET ajst-32233 79 38 complex complex ADJ ajst-32233 79 39 adjuvant adjuvant NOUN ajst-32233 79 40 , , PUNCT ajst-32233 79 41 indicating indicate VERB ajst-32233 79 42 that that SCONJ ajst-32233 79 43 acrylate acrylate NOUN ajst-32233 79 44 hydrogel hydrogel NOUN ajst-32233 79 45 complex complex NOUN ajst-32233 79 46 adjuvant adjuvant NOUN ajst-32233 79 47 has have VERB ajst-32233 79 48 good good ADJ ajst-32233 79 49 injectability injectability NOUN ajst-32233 79 50 . . PUNCT ajst-32233 80 1 in in ADP ajst-32233 80 2 figure figure NOUN ajst-32233 80 3 6b 6b NOUN ajst-32233 80 4 , , PUNCT ajst-32233 80 5 g g NOUN ajst-32233 80 6 ´ ´ NOUN ajst-32233 80 7 represents represent VERB ajst-32233 80 8 storage storage NOUN ajst-32233 80 9 modulus modulus NOUN ajst-32233 80 10 , , PUNCT ajst-32233 80 11 g g DET ajst-32233 80 12 ´ ´ NOUN ajst-32233 80 13 ´ ´ NOUN ajst-32233 80 14 represents represent VERB ajst-32233 80 15 loss loss NOUN ajst-32233 80 16 modulus modulus NOUN ajst-32233 80 17 , , PUNCT ajst-32233 80 18 and and CCONJ ajst-32233 80 19 with with ADP ajst-32233 80 20 increasing increase VERB ajst-32233 80 21 strain strain NOUN ajst-32233 80 22 , , PUNCT ajst-32233 80 23 amplitude amplitude NOUN ajst-32233 80 24 increases increase NOUN ajst-32233 80 25 , , PUNCT ajst-32233 80 26 and and CCONJ ajst-32233 80 27 storage storage NOUN ajst-32233 80 28 modulus modulus NOUN ajst-32233 80 29 decreases decrease NOUN ajst-32233 80 30 for for ADP ajst-32233 80 31 pnoe pnoe NOUN ajst-32233 80 32 and and CCONJ ajst-32233 80 33 ahcp ahcp NOUN ajst-32233 80 34 . . PUNCT ajst-32233 81 1 when when SCONJ ajst-32233 81 2 the the DET ajst-32233 81 3 critical critical ADJ ajst-32233 81 4 strain strain NOUN ajst-32233 81 5 is be AUX ajst-32233 81 6 exceeded exceed VERB ajst-32233 81 7 , , PUNCT ajst-32233 81 8 5 5 NUM ajst-32233 81 9 g´