id	sid	tid	token	lemma	pos
ajst-32578	1	1	232	232	NUM
ajst-32578	1	2	expression	expression	NOUN
ajst-32578	1	3	and	and	CCONJ
ajst-32578	1	4	identification	identification	NOUN
ajst-32578	1	5	of	of	ADP
ajst-32578	1	6	poliovirus	poliovirus	NOUN
ajst-32578	1	7	(	(	PUNCT
ajst-32578	1	8	type	type	NOUN
ajst-32578	1	9	1	1	NUM
ajst-32578	1	10	)	)	PUNCT
ajst-32578	1	11	immune	immune	ADJ
ajst-32578	1	12	antigen	antigen	NOUN
ajst-32578	1	13	in	in	ADP
ajst-32578	1	14	escherichia	escherichia	NOUN
ajst-32578	1	15	coli	coli	NOUN
ajst-32578	1	16	kexin	kexin	PROPN
ajst-32578	1	17	li	li	PROPN
ajst-32578	1	18	chengdu	chengdu	PROPN
ajst-32578	1	19	foreign	foreign	PROPN
ajst-32578	1	20	languages	languages	PROPN
ajst-32578	1	21	school	school	NOUN
ajst-32578	1	22	,	,	PUNCT
ajst-32578	1	23	chengdu	chengdu	PROPN
ajst-32578	1	24	,	,	PUNCT
ajst-32578	1	25	sichuan	sichuan	PROPN
ajst-32578	1	26	,	,	PUNCT
ajst-32578	1	27	china	china	PROPN
ajst-32578	1	28	kerry.li1@icloud.com	kerry.li1@icloud.com	PROPN
ajst-32578	1	29	abstract	abstract	PROPN
ajst-32578	1	30	.	.	PUNCT
ajst-32578	2	1	this	this	DET
ajst-32578	2	2	study	study	NOUN
ajst-32578	2	3	aimed	aim	VERB
ajst-32578	2	4	to	to	PART
ajst-32578	2	5	achieve	achieve	VERB
ajst-32578	2	6	high	high	ADJ
ajst-32578	2	7	-	-	PUNCT
ajst-32578	2	8	level	level	NOUN
ajst-32578	2	9	expression	expression	NOUN
ajst-32578	2	10	of	of	ADP
ajst-32578	2	11	the	the	DET
ajst-32578	2	12	key	key	ADJ
ajst-32578	2	13	immunogenic	immunogenic	ADJ
ajst-32578	2	14	epitope	epitope	NOUN
ajst-32578	2	15	of	of	ADP
ajst-32578	2	16	poliovirus	poliovirus	NOUN
ajst-32578	2	17	type	type	NOUN
ajst-32578	2	18	1	1	NUM
ajst-32578	2	19	(	(	PUNCT
ajst-32578	2	20	pv1	pv1	NOUN
ajst-32578	2	21	)	)	PUNCT
ajst-32578	2	22	vp1	vp1	NOUN
ajst-32578	2	23	in	in	ADP
ajst-32578	2	24	an	an	DET
ajst-32578	2	25	*	*	ADJ
ajst-32578	2	26	escherichia	escherichia	NOUN
ajst-32578	2	27	coli	coli	NOUN
ajst-32578	2	28	*	*	NOUN
ajst-32578	2	29	system	system	NOUN
ajst-32578	2	30	and	and	CCONJ
ajst-32578	2	31	to	to	PART
ajst-32578	2	32	characterize	characterize	VERB
ajst-32578	2	33	its	its	PRON
ajst-32578	2	34	antigenicity	antigenicity	NOUN
ajst-32578	2	35	,	,	PUNCT
ajst-32578	2	36	thereby	thereby	ADV
ajst-32578	2	37	laying	lay	VERB
ajst-32578	2	38	the	the	DET
ajst-32578	2	39	groundwork	groundwork	NOUN
ajst-32578	2	40	for	for	ADP
ajst-32578	2	41	a	a	DET
ajst-32578	2	42	safe	safe	ADJ
ajst-32578	2	43	,	,	PUNCT
ajst-32578	2	44	low	low	ADJ
ajst-32578	2	45	-	-	PUNCT
ajst-32578	2	46	cost	cost	NOUN
ajst-32578	2	47	,	,	PUNCT
ajst-32578	2	48	and	and	CCONJ
ajst-32578	2	49	non	non	ADJ
ajst-32578	2	50	-	-	ADJ
ajst-32578	2	51	infectious	infectious	ADJ
ajst-32578	2	52	subunit	subunit	NOUN
ajst-32578	2	53	vaccine	vaccine	NOUN
ajst-32578	2	54	.	.	PUNCT
ajst-32578	3	1	a	a	DET
ajst-32578	3	2	recombinant	recombinant	ADJ
ajst-32578	3	3	plasmid	plasmid	NOUN
ajst-32578	3	4	,	,	PUNCT
ajst-32578	3	5	pet-32a	pet-32a	NOUN
ajst-32578	3	6	(	(	PUNCT
ajst-32578	3	7	+	+	NOUN
ajst-32578	3	8	)	)	PUNCT
ajst-32578	3	9	-vp1	-vp1	NOUN
ajst-32578	3	10	,	,	PUNCT
ajst-32578	3	11	encoding	encode	VERB
ajst-32578	3	12	the	the	DET
ajst-32578	3	13	functional	functional	ADJ
ajst-32578	3	14	domain	domain	NOUN
ajst-32578	3	15	of	of	ADP
ajst-32578	3	16	pv1	pv1	NOUN
ajst-32578	3	17	vp1	vp1	NOUN
ajst-32578	3	18	was	be	AUX
ajst-32578	3	19	constructed	construct	VERB
ajst-32578	3	20	and	and	CCONJ
ajst-32578	3	21	transformed	transform	VERB
ajst-32578	3	22	into	into	ADP
ajst-32578	3	23	*	*	PROPN
ajst-32578	3	24	e.	e.	PROPN
ajst-32578	3	25	coli	coli	PROPN
ajst-32578	3	26	*	*	PUNCT
ajst-32578	3	27	bl21(de3	bl21(de3	NOUN
ajst-32578	3	28	)	)	PUNCT
ajst-32578	3	29	.	.	PUNCT
ajst-32578	4	1	following	follow	VERB
ajst-32578	4	2	iptg	iptg	NOUN
ajst-32578	4	3	induction	induction	NOUN
ajst-32578	4	4	,	,	PUNCT
ajst-32578	4	5	the	the	DET
ajst-32578	4	6	43	43	NUM
ajst-32578	4	7	kda	kda	NOUN
ajst-32578	4	8	target	target	NOUN
ajst-32578	4	9	protein	protein	NOUN
ajst-32578	4	10	was	be	AUX
ajst-32578	4	11	purified	purify	VERB
ajst-32578	4	12	by	by	ADP
ajst-32578	4	13	ni	ni	PROPN
ajst-32578	4	14	-	-	PUNCT
ajst-32578	4	15	nta	nta	PROPN
ajst-32578	4	16	affinity	affinity	NOUN
ajst-32578	4	17	chromatofigurey	chromatofigurey	NOUN
ajst-32578	4	18	to	to	ADP
ajst-32578	4	19	>	>	SYM
ajst-32578	4	20	90	90	NUM
ajst-32578	4	21	%	%	NOUN
ajst-32578	4	22	homogeneity	homogeneity	NOUN
ajst-32578	4	23	and	and	CCONJ
ajst-32578	4	24	shown	show	VERB
ajst-32578	4	25	to	to	PART
ajst-32578	4	26	retain	retain	VERB
ajst-32578	4	27	immunoreactivity	immunoreactivity	NOUN
ajst-32578	4	28	.	.	PUNCT
ajst-32578	5	1	the	the	DET
ajst-32578	5	2	established	establish	VERB
ajst-32578	5	3	prokaryotic	prokaryotic	ADJ
ajst-32578	5	4	expression	expression	NOUN
ajst-32578	5	5	platform	platform	NOUN
ajst-32578	5	6	enables	enable	VERB
ajst-32578	5	7	scalable	scalable	ADJ
ajst-32578	5	8	production	production	NOUN
ajst-32578	5	9	of	of	ADP
ajst-32578	5	10	pv	pv	NOUN
ajst-32578	5	11	-	-	PUNCT
ajst-32578	5	12	specific	specific	ADJ
ajst-32578	5	13	antigen	antigen	NOUN
ajst-32578	5	14	,	,	PUNCT
ajst-32578	5	15	providing	provide	VERB
ajst-32578	5	16	a	a	DET
ajst-32578	5	17	robust	robust	ADJ
ajst-32578	5	18	technical	technical	ADJ
ajst-32578	5	19	resource	resource	NOUN
ajst-32578	5	20	for	for	ADP
ajst-32578	5	21	functional	functional	ADJ
ajst-32578	5	22	studies	study	NOUN
ajst-32578	5	23	,	,	PUNCT
ajst-32578	5	24	immunogenicity	immunogenicity	NOUN
ajst-32578	5	25	evaluation	evaluation	NOUN
ajst-32578	5	26	,	,	PUNCT
ajst-32578	5	27	and	and	CCONJ
ajst-32578	5	28	next	next	ADJ
ajst-32578	5	29	-	-	PUNCT
ajst-32578	5	30	generation	generation	NOUN
ajst-32578	5	31	vaccine	vaccine	NOUN
ajst-32578	5	32	development	development	NOUN
ajst-32578	5	33	that	that	PRON
ajst-32578	5	34	will	will	AUX
ajst-32578	5	35	contribute	contribute	VERB
ajst-32578	5	36	to	to	ADP
ajst-32578	5	37	global	global	ADJ
ajst-32578	5	38	polio	polio	NOUN
ajst-32578	5	39	eradication	eradication	NOUN
ajst-32578	5	40	efforts	effort	NOUN
ajst-32578	5	41	.	.	PUNCT
ajst-32578	6	1	keywords	keyword	NOUN
ajst-32578	6	2	:	:	PUNCT
ajst-32578	6	3	poliovirus	poliovirus	NOUN
ajst-32578	6	4	;	;	PUNCT
ajst-32578	6	5	vp1	vp1	X
ajst-32578	6	6	protein	protein	NOUN
ajst-32578	6	7	;	;	PUNCT
ajst-32578	6	8	escherichia	escherichia	NOUN
ajst-32578	6	9	coli	coli	NOUN
ajst-32578	6	10	;	;	PUNCT
ajst-32578	6	11	prokaryotic	prokaryotic	ADJ
ajst-32578	6	12	expression	expression	NOUN
ajst-32578	6	13	;	;	PUNCT
ajst-32578	6	14	sds	sds	NOUN
ajst-32578	6	15	-	-	PUNCT
ajst-32578	6	16	page	page	NOUN
ajst-32578	6	17	.	.	PUNCT
ajst-32578	7	1	1	1	X
ajst-32578	7	2	.	.	X
ajst-32578	7	3	introduction	introduction	NOUN
ajst-32578	7	4	poliomyelitis	poliomyelitis	PROPN
ajst-32578	7	5	(	(	PUNCT
ajst-32578	7	6	commonly	commonly	ADV
ajst-32578	7	7	known	know	VERB
ajst-32578	7	8	as	as	ADP
ajst-32578	7	9	polio	polio	NOUN
ajst-32578	7	10	)	)	PUNCT
ajst-32578	7	11	is	be	AUX
ajst-32578	7	12	an	an	DET
ajst-32578	7	13	acute	acute	ADJ
ajst-32578	7	14	infectious	infectious	ADJ
ajst-32578	7	15	disease	disease	NOUN
ajst-32578	7	16	caused	cause	VERB
ajst-32578	7	17	by	by	ADP
ajst-32578	7	18	the	the	DET
ajst-32578	7	19	poliovirus	poliovirus	NOUN
ajst-32578	7	20	,	,	PUNCT
ajst-32578	7	21	a	a	DET
ajst-32578	7	22	member	member	NOUN
ajst-32578	7	23	of	of	ADP
ajst-32578	7	24	the	the	DET
ajst-32578	7	25	enterovirus	enterovirus	NOUN
ajst-32578	7	26	genus	genus	NOUN
ajst-32578	7	27	within	within	ADP
ajst-32578	7	28	the	the	DET
ajst-32578	7	29	picornaviridae	picornaviridae	NOUN
ajst-32578	7	30	family	family	NOUN
ajst-32578	7	31	.	.	PUNCT
ajst-32578	8	1	the	the	DET
ajst-32578	8	2	virus	virus	NOUN
ajst-32578	8	3	primarily	primarily	ADV
ajst-32578	8	4	targets	target	VERB
ajst-32578	8	5	neurons	neuron	NOUN
ajst-32578	8	6	,	,	PUNCT
ajst-32578	8	7	causing	cause	VERB
ajst-32578	8	8	degeneration	degeneration	NOUN
ajst-32578	8	9	and	and	CCONJ
ajst-32578	8	10	necrosis	necrosis	NOUN
ajst-32578	8	11	that	that	PRON
ajst-32578	8	12	may	may	AUX
ajst-32578	8	13	lead	lead	VERB
ajst-32578	8	14	to	to	ADP
ajst-32578	8	15	paralysis	paralysis	NOUN
ajst-32578	8	16	or	or	CCONJ
ajst-32578	8	17	even	even	ADV
ajst-32578	8	18	fatal	fatal	ADJ
ajst-32578	8	19	respiratory	respiratory	ADJ
ajst-32578	8	20	muscle	muscle	NOUN
ajst-32578	8	21	paralysis	paralysis	NOUN
ajst-32578	8	22	in	in	ADP
ajst-32578	8	23	severe	severe	ADJ
ajst-32578	8	24	cases	case	NOUN
ajst-32578	8	25	.	.	PUNCT
ajst-32578	9	1	humans	human	NOUN
ajst-32578	9	2	are	be	AUX
ajst-32578	9	3	the	the	DET
ajst-32578	9	4	viruss	viruss	NOUN
ajst-32578	9	5	only	only	ADV
ajst-32578	9	6	natural	natural	ADJ
ajst-32578	9	7	host	host	NOUN
ajst-32578	9	8	,	,	PUNCT
ajst-32578	9	9	with	with	ADP
ajst-32578	9	10	clinical	clinical	ADJ
ajst-32578	9	11	manifestations	manifestation	NOUN
ajst-32578	9	12	including	include	VERB
ajst-32578	9	13	asymmetrical	asymmetrical	ADJ
ajst-32578	9	14	flaccid	flaccid	ADJ
ajst-32578	9	15	paralysis	paralysis	NOUN
ajst-32578	9	16	(	(	PUNCT
ajst-32578	9	17	hence	hence	ADV
ajst-32578	9	18	the	the	DET
ajst-32578	9	19	name	name	NOUN
ajst-32578	9	20	"	"	PUNCT
ajst-32578	9	21	polio	polio	NOUN
ajst-32578	9	22	"	"	PUNCT
ajst-32578	9	23	)	)	PUNCT
ajst-32578	9	24	after	after	ADP
ajst-32578	9	25	infection	infection	NOUN
ajst-32578	9	26	.	.	PUNCT
ajst-32578	10	1	polioviruses	poliovirus	NOUN
ajst-32578	10	2	are	be	AUX
ajst-32578	10	3	classified	classify	VERB
ajst-32578	10	4	into	into	ADP
ajst-32578	10	5	three	three	NUM
ajst-32578	10	6	serotypes	serotype	NOUN
ajst-32578	10	7	:	:	PUNCT
ajst-32578	10	8	1	1	NUM
ajst-32578	10	9	,	,	PUNCT
ajst-32578	10	10	2	2	NUM
ajst-32578	10	11	,	,	PUNCT
ajst-32578	10	12	and	and	CCONJ
ajst-32578	10	13	3	3	NUM
ajst-32578	10	14	,	,	PUNCT
ajst-32578	10	15	with	with	ADP
ajst-32578	10	16	type	type	NOUN
ajst-32578	10	17	1	1	NUM
ajst-32578	10	18	being	be	AUX
ajst-32578	10	19	the	the	DET
ajst-32578	10	20	most	most	ADV
ajst-32578	10	21	common	common	ADJ
ajst-32578	10	22	cause	cause	NOUN
ajst-32578	10	23	of	of	ADP
ajst-32578	10	24	paralysis	paralysis	NOUN
ajst-32578	10	25	.	.	PUNCT
ajst-32578	11	1	most	most	ADJ
ajst-32578	11	2	infected	infect	VERB
ajst-32578	11	3	individuals	individual	NOUN
ajst-32578	11	4	experience	experience	VERB
ajst-32578	11	5	asymptomatic	asymptomatic	ADJ
ajst-32578	11	6	infections	infection	NOUN
ajst-32578	11	7	,	,	PUNCT
ajst-32578	11	8	while	while	SCONJ
ajst-32578	11	9	some	some	PRON
ajst-32578	11	10	may	may	AUX
ajst-32578	11	11	develop	develop	VERB
ajst-32578	11	12	symptoms	symptom	NOUN
ajst-32578	11	13	like	like	ADP
ajst-32578	11	14	fever	fever	NOUN
ajst-32578	11	15	,	,	PUNCT
ajst-32578	11	16	headache	headache	NOUN
ajst-32578	11	17	,	,	PUNCT
ajst-32578	11	18	sore	sore	ADJ
ajst-32578	11	19	throat	throat	NOUN
ajst-32578	11	20	,	,	PUNCT
ajst-32578	11	21	and	and	CCONJ
ajst-32578	11	22	fatigue	fatigue	NOUN
ajst-32578	12	1	[	[	X
ajst-32578	12	2	1	1	NUM
ajst-32578	12	3	]	]	PUNCT
ajst-32578	12	4	.	.	PUNCT
ajst-32578	13	1	polio	polio	NOUN
ajst-32578	13	2	is	be	AUX
ajst-32578	13	3	endemic	endemic	ADJ
ajst-32578	13	4	throughout	throughout	ADP
ajst-32578	13	5	temperate	temperate	ADJ
ajst-32578	13	6	regions	region	NOUN
ajst-32578	13	7	,	,	PUNCT
ajst-32578	13	8	with	with	ADP
ajst-32578	13	9	higher	high	ADJ
ajst-32578	13	10	incidence	incidence	NOUN
ajst-32578	13	11	rates	rate	NOUN
ajst-32578	13	12	in	in	ADP
ajst-32578	13	13	summer	summer	NOUN
ajst-32578	13	14	and	and	CCONJ
ajst-32578	13	15	autumn	autumn	NOUN
ajst-32578	13	16	,	,	PUNCT
ajst-32578	13	17	while	while	SCONJ
ajst-32578	13	18	tropical	tropical	ADJ
ajst-32578	13	19	areas	area	NOUN
ajst-32578	13	20	show	show	VERB
ajst-32578	13	21	similar	similar	ADJ
ajst-32578	13	22	seasonal	seasonal	ADJ
ajst-32578	13	23	patterns	pattern	NOUN
ajst-32578	13	24	.	.	PUNCT
ajst-32578	14	1	the	the	DET
ajst-32578	14	2	global	global	ADJ
ajst-32578	14	3	polio	polio	NOUN
ajst-32578	14	4	prevalence	prevalence	NOUN
ajst-32578	14	5	has	have	AUX
ajst-32578	14	6	significantly	significantly	ADV
ajst-32578	14	7	decreased	decrease	VERB
ajst-32578	14	8	following	follow	VERB
ajst-32578	14	9	the	the	DET
ajst-32578	14	10	widespread	widespread	ADJ
ajst-32578	14	11	use	use	NOUN
ajst-32578	14	12	of	of	ADP
ajst-32578	14	13	oral	oral	ADJ
ajst-32578	14	14	polio	polio	NOUN
ajst-32578	14	15	vaccine	vaccine	NOUN
ajst-32578	14	16	(	(	PUNCT
ajst-32578	14	17	opv	opv	PROPN
ajst-32578	14	18	)	)	PUNCT
ajst-32578	14	19	.	.	PUNCT
ajst-32578	15	1	currently	currently	ADV
ajst-32578	15	2	,	,	PUNCT
ajst-32578	15	3	chinas	china	NOUN
ajst-32578	15	4	vaccination	vaccination	NOUN
ajst-32578	15	5	regimen	regiman	NOUN
ajst-32578	15	6	primarily	primarily	ADV
ajst-32578	15	7	includes	include	VERB
ajst-32578	15	8	inactivated	inactivated	ADJ
ajst-32578	15	9	polio	polio	NOUN
ajst-32578	15	10	vaccine	vaccine	NOUN
ajst-32578	15	11	(	(	PUNCT
ajst-32578	15	12	ipv	ipv	PROPN
ajst-32578	15	13	)	)	PUNCT
ajst-32578	15	14	,	,	PUNCT
ajst-32578	15	15	bivalent	bivalent	ADJ
ajst-32578	15	16	oral	oral	ADJ
ajst-32578	15	17	poliovirus	poliovirus	NOUN
ajst-32578	15	18	vaccine	vaccine	NOUN
ajst-32578	15	19	(	(	PUNCT
ajst-32578	15	20	bopv	bopv	PROPN
ajst-32578	15	21	)	)	PUNCT
ajst-32578	15	22	,	,	PUNCT
ajst-32578	15	23	and	and	CCONJ
ajst-32578	15	24	combined	combined	ADJ
ajst-32578	15	25	vaccines	vaccine	NOUN
ajst-32578	15	26	containing	contain	VERB
ajst-32578	15	27	ipv	ipv	PROPN
ajst-32578	15	28	.	.	PUNCT
ajst-32578	16	1	however	however	ADV
ajst-32578	16	2	,	,	PUNCT
ajst-32578	16	3	opv	opv	PROPN
ajst-32578	16	4	administration	administration	NOUN
ajst-32578	16	5	carries	carry	VERB
ajst-32578	16	6	risks	risk	NOUN
ajst-32578	16	7	such	such	ADJ
ajst-32578	16	8	as	as	ADP
ajst-32578	16	9	vaccineassociated	vaccineassociate	VERB
ajst-32578	16	10	paralytic	paralytic	ADJ
ajst-32578	16	11	poliomyelitis	poliomyelitis	NOUN
ajst-32578	16	12	(	(	PUNCT
ajst-32578	16	13	vapp	vapp	NOUN
ajst-32578	16	14	)	)	PUNCT
ajst-32578	16	15	and	and	CCONJ
ajst-32578	16	16	vaccine	vaccine	NOUN
ajst-32578	16	17	-	-	PUNCT
ajst-32578	16	18	derived	derive	VERB
ajst-32578	16	19	poliovirus	poliovirus	NOUN
ajst-32578	16	20	(	(	PUNCT
ajst-32578	16	21	vdpv	vdpv	PROPN
ajst-32578	16	22	)	)	PUNCT
ajst-32578	16	23	.	.	PUNCT
ajst-32578	17	1	consequently	consequently	ADV
ajst-32578	17	2	,	,	PUNCT
ajst-32578	17	3	developing	develop	VERB
ajst-32578	17	4	safer	safe	ADJ
ajst-32578	17	5	new	new	ADJ
ajst-32578	17	6	vaccines	vaccine	NOUN
ajst-32578	17	7	has	have	AUX
ajst-32578	17	8	become	become	VERB
ajst-32578	17	9	a	a	DET
ajst-32578	17	10	key	key	ADJ
ajst-32578	17	11	focus	focus	NOUN
ajst-32578	17	12	of	of	ADP
ajst-32578	17	13	current	current	ADJ
ajst-32578	17	14	research	research	NOUN
ajst-32578	17	15	[	[	X
ajst-32578	17	16	2	2	NUM
ajst-32578	17	17	-	-	SYM
ajst-32578	17	18	3	3	NUM
ajst-32578	17	19	]	]	PUNCT
ajst-32578	17	20	.	.	PUNCT
ajst-32578	18	1	the	the	DET
ajst-32578	18	2	varicella	varicella	NOUN
ajst-32578	18	3	-	-	PUNCT
ajst-32578	18	4	zoster	zoster	NOUN
ajst-32578	18	5	virus	virus	NOUN
ajst-32578	18	6	(	(	PUNCT
ajst-32578	18	7	vzv	vzv	PROPN
ajst-32578	18	8	)	)	PUNCT
ajst-32578	18	9	particles	particle	NOUN
ajst-32578	18	10	exhibit	exhibit	VERB
ajst-32578	18	11	an	an	DET
ajst-32578	18	12	icosahedral	icosahedral	ADJ
ajst-32578	18	13	symmetry	symmetry	NOUN
ajst-32578	18	14	structure	structure	NOUN
ajst-32578	18	15	,	,	PUNCT
ajst-32578	18	16	measuring	measure	VERB
ajst-32578	18	17	approximately	approximately	ADV
ajst-32578	18	18	20	20	NUM
ajst-32578	18	19	-	-	SYM
ajst-32578	18	20	30	30	NUM
ajst-32578	18	21	nm	nm	NOUN
ajst-32578	18	22	in	in	ADP
ajst-32578	18	23	diameter	diameter	NOUN
ajst-32578	18	24	and	and	CCONJ
ajst-32578	18	25	lacking	lack	VERB
ajst-32578	18	26	an	an	DET
ajst-32578	18	27	envelope	envelope	NOUN
ajst-32578	18	28	.	.	PUNCT
ajst-32578	19	1	the	the	DET
ajst-32578	19	2	viral	viral	ADJ
ajst-32578	19	3	capsid	capsid	NOUN
ajst-32578	19	4	consists	consist	VERB
ajst-32578	19	5	of	of	ADP
ajst-32578	19	6	four	four	NUM
ajst-32578	19	7	structural	structural	ADJ
ajst-32578	19	8	proteins	protein	NOUN
ajst-32578	19	9	:	:	PUNCT
ajst-32578	19	10	vp1	vp1	NOUN
ajst-32578	19	11	,	,	PUNCT
ajst-32578	19	12	vp2	vp2	NOUN
ajst-32578	19	13	,	,	PUNCT
ajst-32578	19	14	vp3	vp3	PROPN
ajst-32578	19	15	,	,	PUNCT
ajst-32578	19	16	and	and	CCONJ
ajst-32578	19	17	vp4	vp4	VERB
ajst-32578	19	18	.	.	PUNCT
ajst-32578	20	1	notably	notably	ADV
ajst-32578	20	2	,	,	PUNCT
ajst-32578	20	3	vp1	vp1	PROPN
ajst-32578	20	4	serves	serve	VERB
ajst-32578	20	5	as	as	ADP
ajst-32578	20	6	the	the	DET
ajst-32578	20	7	primary	primary	ADJ
ajst-32578	20	8	surface	surface	NOUN
ajst-32578	20	9	protein	protein	NOUN
ajst-32578	20	10	,	,	PUNCT
ajst-32578	20	11	containing	contain	VERB
ajst-32578	20	12	antigenic	antigenic	ADJ
ajst-32578	20	13	epitopes	epitope	NOUN
ajst-32578	20	14	that	that	PRON
ajst-32578	20	15	induce	induce	VERB
ajst-32578	20	16	neutralizing	neutralize	VERB
ajst-32578	20	17	antibodies	antibody	NOUN
ajst-32578	20	18	.	.	PUNCT
ajst-32578	21	1	this	this	DET
ajst-32578	21	2	protein	protein	NOUN
ajst-32578	21	3	interacts	interact	VERB
ajst-32578	21	4	with	with	ADP
ajst-32578	21	5	host	host	NOUN
ajst-32578	21	6	cell	cell	NOUN
ajst-32578	21	7	receptors	receptor	NOUN
ajst-32578	21	8	and	and	CCONJ
ajst-32578	21	9	plays	play	VERB
ajst-32578	21	10	a	a	DET
ajst-32578	21	11	crucial	crucial	ADJ
ajst-32578	21	12	role	role	NOUN
ajst-32578	21	13	in	in	ADP
ajst-32578	21	14	viral	viral	ADJ
ajst-32578	21	15	pathogenesis	pathogenesis	NOUN
ajst-32578	21	16	[	[	X
ajst-32578	21	17	4	4	NUM
ajst-32578	21	18	]	]	PUNCT
ajst-32578	21	19	.	.	PUNCT
ajst-32578	22	1	in	in	ADP
ajst-32578	22	2	this	this	DET
ajst-32578	22	3	study	study	NOUN
ajst-32578	22	4	,	,	PUNCT
ajst-32578	22	5	the	the	DET
ajst-32578	22	6	recombinant	recombinant	ADJ
ajst-32578	22	7	expression	expression	NOUN
ajst-32578	22	8	vector	vector	NOUN
ajst-32578	22	9	containing	contain	VERB
ajst-32578	22	10	vp1	vp1	NOUN
ajst-32578	22	11	antigen	antigen	ADJ
ajst-32578	22	12	epitope	epitope	NOUN
ajst-32578	22	13	was	be	AUX
ajst-32578	22	14	constructed	construct	VERB
ajst-32578	22	15	by	by	ADP
ajst-32578	22	16	molecular	molecular	ADJ
ajst-32578	22	17	cloning	cloning	NOUN
ajst-32578	22	18	technology	technology	NOUN
ajst-32578	22	19	[	[	X
ajst-32578	22	20	5	5	NUM
ajst-32578	22	21	]	]	PUNCT
ajst-32578	22	22	,	,	PUNCT
ajst-32578	22	23	and	and	CCONJ
ajst-32578	22	24	the	the	DET
ajst-32578	22	25	target	target	NOUN
ajst-32578	22	26	protein	protein	NOUN
ajst-32578	22	27	was	be	AUX
ajst-32578	22	28	expressed	express	VERB
ajst-32578	22	29	and	and	CCONJ
ajst-32578	22	30	purified	purify	VERB
ajst-32578	22	31	efficiently	efficiently	ADV
ajst-32578	22	32	by	by	ADP
ajst-32578	22	33	using	use	VERB
ajst-32578	22	34	the	the	DET
ajst-32578	22	35	e.	e.	PROPN
ajst-32578	22	36	coli	coli	NOUN
ajst-32578	22	37	expression	expression	NOUN
ajst-32578	22	38	system	system	NOUN
ajst-32578	22	39	,	,	PUNCT
ajst-32578	22	40	which	which	PRON
ajst-32578	22	41	provided	provide	VERB
ajst-32578	22	42	experimental	experimental	ADJ
ajst-32578	22	43	basis	basis	NOUN
ajst-32578	22	44	for	for	ADP
ajst-32578	22	45	the	the	DET
ajst-32578	22	46	subsequent	subsequent	ADJ
ajst-32578	22	47	subunit	subunit	NOUN
ajst-32578	22	48	vaccine	vaccine	NOUN
ajst-32578	22	49	development	development	NOUN
ajst-32578	22	50	.	.	PUNCT
ajst-32578	23	1	233	233	NUM
ajst-32578	23	2	2	2	NUM
ajst-32578	23	3	.	.	PUNCT
ajst-32578	23	4	materials	material	NOUN
ajst-32578	23	5	and	and	CCONJ
ajst-32578	23	6	methods	method	NOUN
ajst-32578	23	7	:	:	PUNCT
ajst-32578	23	8	2.1	2.1	NUM
ajst-32578	23	9	materials	material	NOUN
ajst-32578	23	10	:	:	PUNCT
ajst-32578	23	11	2.1.1	2.1.1	NUM
ajst-32578	23	12	strains	strain	NOUN
ajst-32578	23	13	and	and	CCONJ
ajst-32578	23	14	vectors	vector	NOUN
ajst-32578	23	15	escherichia	escherichia	NOUN
ajst-32578	23	16	coli	coli	NOUN
ajst-32578	23	17	dh5α	dh5α	PROPN
ajst-32578	23	18	(	(	PUNCT
ajst-32578	23	19	cloning	cloning	NOUN
ajst-32578	23	20	strain	strain	NOUN
ajst-32578	23	21	)	)	PUNCT
ajst-32578	23	22	,	,	PUNCT
ajst-32578	23	23	bl21(de3	bl21(de3	VERB
ajst-32578	23	24	)	)	PUNCT
ajst-32578	23	25	(	(	PUNCT
ajst-32578	23	26	expression	expression	NOUN
ajst-32578	23	27	strain	strain	NOUN
ajst-32578	23	28	)	)	PUNCT
ajst-32578	23	29	;	;	PUNCT
ajst-32578	23	30	expression	expression	NOUN
ajst-32578	23	31	vector	vector	NOUN
ajst-32578	23	32	pet32a	pet32a	NOUN
ajst-32578	23	33	(	(	PUNCT
ajst-32578	23	34	+	+	NOUN
ajst-32578	23	35	)	)	PUNCT
ajst-32578	23	36	2.1.2	2.1.2	NUM
ajst-32578	23	37	reagents	reagent	NOUN
ajst-32578	23	38	and	and	CCONJ
ajst-32578	23	39	kits	kit	NOUN
ajst-32578	23	40	enzymes	enzyme	NOUN
ajst-32578	23	41	:	:	PUNCT
ajst-32578	23	42	t4	t4	PROPN
ajst-32578	23	43	dna	dna	PROPN
ajst-32578	23	44	ligase	ligase	PROPN
ajst-32578	23	45	,	,	PUNCT
ajst-32578	23	46	restriction	restriction	NOUN
ajst-32578	23	47	endonucleases	endonuclease	NOUN
ajst-32578	23	48	(	(	PUNCT
ajst-32578	23	49	ecorv	ecorv	NOUN
ajst-32578	23	50	,	,	PUNCT
ajst-32578	23	51	xhoi	xhoi	ADJ
ajst-32578	23	52	)	)	PUNCT
ajst-32578	23	53	,	,	PUNCT
ajst-32578	23	54	taq	taq	PROPN
ajst-32578	23	55	dna	dna	PROPN
ajst-32578	23	56	polymerase	polymerase	NOUN
ajst-32578	23	57	;	;	PUNCT
ajst-32578	23	58	kits	kit	NOUN
ajst-32578	23	59	:	:	PUNCT
ajst-32578	23	60	standard	standard	ADJ
ajst-32578	23	61	agarose	agarose	NOUN
ajst-32578	23	62	gel	gel	NOUN
ajst-32578	23	63	dna	dna	PROPN
ajst-32578	23	64	recovery	recovery	NOUN
ajst-32578	23	65	kit	kit	NOUN
ajst-32578	23	66	(	(	PUNCT
ajst-32578	23	67	tiangen	tiangen	NOUN
ajst-32578	23	68	dp209	dp209	NOUN
ajst-32578	23	69	-	-	PUNCT
ajst-32578	23	70	02	02	NUM
ajst-32578	23	71	)	)	PUNCT
ajst-32578	23	72	,	,	PUNCT
ajst-32578	23	73	non	non	ADJ
ajst-32578	23	74	-	-	ADJ
ajst-32578	23	75	toxic	toxic	ADJ
ajst-32578	23	76	plasmid	plasmid	ADJ
ajst-32578	23	77	extraction	extraction	NOUN
ajst-32578	23	78	medium	medium	NOUN
ajst-32578	23	79	(	(	PUNCT
ajst-32578	23	80	tiangen	tiangen	NOUN
ajst-32578	23	81	dp118	dp118	PROPN
ajst-32578	23	82	-	-	NUM
ajst-32578	23	83	02	02	NUM
ajst-32578	23	84	)	)	PUNCT
ajst-32578	23	85	;	;	PUNCT
ajst-32578	23	86	media	medium	NOUN
ajst-32578	23	87	&	&	CCONJ
ajst-32578	23	88	reagents	reagent	NOUN
ajst-32578	23	89	:	:	PUNCT
ajst-32578	23	90	lb	lb	NUM
ajst-32578	23	91	culture	culture	NOUN
ajst-32578	23	92	medium	medium	NOUN
ajst-32578	23	93	,	,	PUNCT
ajst-32578	23	94	kanamycin	kanamycin	PROPN
ajst-32578	23	95	,	,	PUNCT
ajst-32578	23	96	iptg	iptg	PROPN
ajst-32578	23	97	,	,	PUNCT
ajst-32578	23	98	sdspage	sdspage	NOUN
ajst-32578	23	99	reagents	reagent	NOUN
ajst-32578	23	100	,	,	PUNCT
ajst-32578	23	101	coomassie	coomassie	ADJ
ajst-32578	23	102	brilliant	brilliant	ADJ
ajst-32578	23	103	blue	blue	ADJ
ajst-32578	23	104	stain	stain	NOUN
ajst-32578	23	105	,	,	PUNCT
ajst-32578	23	106	ni	ni	PROPN
ajst-32578	23	107	-	-	PUNCT
ajst-32578	23	108	nta	nta	PROPN
ajst-32578	23	109	affinity	affinity	NOUN
ajst-32578	23	110	chromatofigurey	chromatofigurey	PROPN
ajst-32578	23	111	column	column	NOUN
ajst-32578	23	112	;	;	PUNCT
ajst-32578	23	113	others	other	NOUN
ajst-32578	23	114	:	:	PUNCT
ajst-32578	23	115	broth	broth	NOUN
ajst-32578	23	116	culture	culture	NOUN
ajst-32578	23	117	medium	medium	NOUN
ajst-32578	23	118	,	,	PUNCT
ajst-32578	23	119	peptone	peptone	NOUN
ajst-32578	23	120	,	,	PUNCT
ajst-32578	23	121	yeast	yeast	NOUN
ajst-32578	23	122	extract	extract	NOUN
ajst-32578	23	123	powder	powder	NOUN
ajst-32578	23	124	.	.	PUNCT
ajst-32578	24	1	2.2	2.2	NUM
ajst-32578	24	2	experimental	experimental	ADJ
ajst-32578	24	3	method	method	NOUN
ajst-32578	24	4	2.2.1	2.2.1	NUM
ajst-32578	24	5	construction	construction	NOUN
ajst-32578	24	6	of	of	ADP
ajst-32578	24	7	recombinant	recombinant	ADJ
ajst-32578	24	8	expression	expression	NOUN
ajst-32578	24	9	vector	vector	NOUN
ajst-32578	24	10	(	(	PUNCT
ajst-32578	24	11	figure	figure	NOUN
ajst-32578	24	12	1	1	NUM
ajst-32578	24	13	)	)	SYM
ajst-32578	24	14	1	1	NUM
ajst-32578	24	15	)	)	PUNCT
ajst-32578	24	16	target	target	NOUN
ajst-32578	24	17	gene	gene	NOUN
ajst-32578	24	18	amplification	amplification	NOUN
ajst-32578	24	19	:	:	PUNCT
ajst-32578	24	20	design	design	NOUN
ajst-32578	24	21	primers	primer	NOUN
ajst-32578	24	22	based	base	VERB
ajst-32578	24	23	on	on	ADP
ajst-32578	24	24	the	the	DET
ajst-32578	24	25	vp1	vp1	NOUN
ajst-32578	24	26	protein	protein	NOUN
ajst-32578	24	27	epitope	epitope	NOUN
ajst-32578	24	28	sequence	sequence	NOUN
ajst-32578	24	29	of	of	ADP
ajst-32578	24	30	poliovirus	poliovirus	NOUN
ajst-32578	24	31	,	,	PUNCT
ajst-32578	24	32	incorporating	incorporate	VERB
ajst-32578	24	33	ecorv	ecorv	NOUN
ajst-32578	24	34	and	and	CCONJ
ajst-32578	24	35	xhoi	xhoi	ADJ
ajst-32578	24	36	restriction	restriction	NOUN
ajst-32578	24	37	enzyme	enzyme	NOUN
ajst-32578	24	38	cleavage	cleavage	NOUN
ajst-32578	24	39	sites	site	NOUN
ajst-32578	24	40	at	at	ADP
ajst-32578	24	41	both	both	DET
ajst-32578	24	42	ends	end	NOUN
ajst-32578	24	43	,	,	PUNCT
ajst-32578	24	44	followed	follow	VERB
ajst-32578	24	45	by	by	ADP
ajst-32578	24	46	pcr	pcr	ADJ
ajst-32578	24	47	amplification	amplification	NOUN
ajst-32578	24	48	of	of	ADP
ajst-32578	24	49	the	the	DET
ajst-32578	24	50	target	target	NOUN
ajst-32578	24	51	gene	gene	NOUN
ajst-32578	24	52	.	.	PUNCT
ajst-32578	25	1	2	2	X
ajst-32578	25	2	)	)	PUNCT
ajst-32578	25	3	restriction	restriction	NOUN
ajst-32578	25	4	enzyme	enzyme	NOUN
ajst-32578	25	5	digestion	digestion	NOUN
ajst-32578	25	6	and	and	CCONJ
ajst-32578	25	7	ligation	ligation	NOUN
ajst-32578	25	8	:	:	PUNCT
ajst-32578	25	9	perform	perform	VERB
ajst-32578	25	10	double	double	ADJ
ajst-32578	25	11	digestion	digestion	NOUN
ajst-32578	25	12	of	of	ADP
ajst-32578	25	13	the	the	DET
ajst-32578	25	14	target	target	NOUN
ajst-32578	25	15	gene	gene	NOUN
ajst-32578	25	16	and	and	CCONJ
ajst-32578	25	17	pet-32a	pet-32a	NOUN
ajst-32578	25	18	(	(	PUNCT
ajst-32578	25	19	+	+	ADJ
ajst-32578	25	20	)	)	PUNCT
ajst-32578	25	21	-vector	-vector	NOUN
ajst-32578	25	22	using	use	VERB
ajst-32578	25	23	ecorv	ecorv	NOUN
ajst-32578	25	24	and	and	CCONJ
ajst-32578	25	25	xhoi	xhoi	ADJ
ajst-32578	25	26	enzymes	enzyme	NOUN
ajst-32578	25	27	.	.	PUNCT
ajst-32578	26	1	construct	construct	VERB
ajst-32578	26	2	the	the	DET
ajst-32578	26	3	recombinant	recombinant	ADJ
ajst-32578	26	4	plasmid	plasmid	ADJ
ajst-32578	26	5	pet-32a	pet-32a	NOUN
ajst-32578	26	6	(	(	PUNCT
ajst-32578	26	7	+	+	NOUN
ajst-32578	26	8	)	)	PUNCT
ajst-32578	26	9	-vp1	-vp1	VERB
ajst-32578	26	10	through	through	ADP
ajst-32578	26	11	t4	t4	PROPN
ajst-32578	26	12	dna	dna	PROPN
ajst-32578	26	13	ligase	ligase	PROPN
ajst-32578	26	14	ligation	ligation	PROPN
ajst-32578	26	15	.	.	PUNCT
ajst-32578	27	1	3	3	X
ajst-32578	27	2	)	)	PUNCT
ajst-32578	27	3	transformation	transformation	NOUN
ajst-32578	27	4	and	and	CCONJ
ajst-32578	27	5	screening	screening	NOUN
ajst-32578	27	6	:	:	PUNCT
ajst-32578	27	7	transform	transform	VERB
ajst-32578	27	8	the	the	DET
ajst-32578	27	9	plasmid	plasmid	NOUN
ajst-32578	27	10	into	into	ADP
ajst-32578	27	11	dh5α	dh5α	PROPN
ajst-32578	27	12	competent	competent	ADJ
ajst-32578	27	13	cells	cell	NOUN
ajst-32578	27	14	.	.	PUNCT
ajst-32578	28	1	screen	screen	NOUN
ajst-32578	28	2	positive	positive	ADJ
ajst-32578	28	3	clones	clone	NOUN
ajst-32578	28	4	using	use	VERB
ajst-32578	28	5	kan	kan	NOUN
ajst-32578	28	6	-	-	PUNCT
ajst-32578	28	7	resistant	resistant	ADJ
ajst-32578	28	8	agar	agar	NOUN
ajst-32578	28	9	plates	plate	NOUN
ajst-32578	28	10	.	.	PUNCT
ajst-32578	29	1	verify	verify	VERB
ajst-32578	29	2	the	the	DET
ajst-32578	29	3	plasmid	plasmid	NOUN
ajst-32578	29	4	construct	construct	NOUN
ajst-32578	29	5	accuracy	accuracy	NOUN
ajst-32578	29	6	through	through	ADP
ajst-32578	29	7	restriction	restriction	NOUN
ajst-32578	29	8	enzyme	enzyme	NOUN
ajst-32578	29	9	digestion	digestion	NOUN
ajst-32578	29	10	and	and	CCONJ
ajst-32578	29	11	gene	gene	NOUN
ajst-32578	29	12	sequencing	sequencing	NOUN
ajst-32578	29	13	.	.	PUNCT
ajst-32578	30	1	a.	a.	NOUN
ajst-32578	30	2	expression	expression	NOUN
ajst-32578	30	3	vector	vector	NOUN
ajst-32578	30	4	map	map	NOUN
ajst-32578	30	5	b.	b.	PROPN
ajst-32578	30	6	expression	expression	NOUN
ajst-32578	30	7	vector	vector	NOUN
ajst-32578	30	8	with	with	ADP
ajst-32578	30	9	multiple	multiple	ADJ
ajst-32578	30	10	cloning	cloning	NOUN
ajst-32578	30	11	sites	site	NOUN
ajst-32578	30	12	figure	figure	VERB
ajst-32578	30	13	1	1	NUM
ajst-32578	30	14	.	.	PUNCT
ajst-32578	30	15	recombinant	recombinant	ADJ
ajst-32578	30	16	expression	expression	NOUN
ajst-32578	30	17	vector	vector	NOUN
ajst-32578	30	18	234	234	NUM
ajst-32578	30	19	2.2.2	2.2.2	NUM
ajst-32578	30	20	plasmid	plasmid	NOUN
ajst-32578	30	21	extraction	extraction	NOUN
ajst-32578	30	22	and	and	CCONJ
ajst-32578	30	23	agarose	agarose	NOUN
ajst-32578	30	24	electrophoresis	electrophoresis	NOUN
ajst-32578	30	25	refer	refer	VERB
ajst-32578	30	26	to	to	ADP
ajst-32578	30	27	the	the	DET
ajst-32578	30	28	instructions	instruction	NOUN
ajst-32578	30	29	for	for	ADP
ajst-32578	30	30	the	the	DET
ajst-32578	30	31	non	non	ADJ
ajst-32578	30	32	-	-	ADJ
ajst-32578	30	33	endotoxin	endotoxin	ADJ
ajst-32578	30	34	plasmid	plasmid	ADJ
ajst-32578	30	35	extraction	extraction	NOUN
ajst-32578	30	36	kit	kit	NOUN
ajst-32578	30	37	(	(	PUNCT
ajst-32578	30	38	tiangen	tiangen	NOUN
ajst-32578	30	39	dp118	dp118	PROPN
ajst-32578	30	40	-	-	NUM
ajst-32578	30	41	02	02	NUM
ajst-32578	30	42	)	)	PUNCT
ajst-32578	30	43	.	.	PUNCT
ajst-32578	31	1	2.2.3	2.2.3	NUM
ajst-32578	31	2	reconstituted	reconstitute	VERB
ajst-32578	31	3	protein	protein	NOUN
ajst-32578	31	4	induced	induce	VERB
ajst-32578	31	5	expression	expression	NOUN
ajst-32578	31	6	1	1	NUM
ajst-32578	31	7	)	)	PUNCT
ajst-32578	31	8	preparation	preparation	NOUN
ajst-32578	31	9	of	of	ADP
ajst-32578	31	10	competent	competent	ADJ
ajst-32578	31	11	cells	cell	NOUN
ajst-32578	31	12	:	:	PUNCT
ajst-32578	31	13	prepare	prepare	VERB
ajst-32578	31	14	bl21(de3	bl21(de3	VERB
ajst-32578	31	15	)	)	PUNCT
ajst-32578	31	16	competent	competent	ADJ
ajst-32578	31	17	cells	cell	NOUN
ajst-32578	31	18	using	use	VERB
ajst-32578	31	19	the	the	DET
ajst-32578	31	20	cacl₂	cacl₂	NOUN
ajst-32578	31	21	method	method	NOUN
ajst-32578	31	22	;	;	PUNCT
ajst-32578	31	23	2	2	X
ajst-32578	31	24	)	)	PUNCT
ajst-32578	31	25	transformation	transformation	NOUN
ajst-32578	31	26	and	and	CCONJ
ajst-32578	31	27	cultivation	cultivation	NOUN
ajst-32578	31	28	:	:	PUNCT
ajst-32578	31	29	transform	transform	VERB
ajst-32578	31	30	recombinant	recombinant	ADJ
ajst-32578	31	31	plasmids	plasmid	NOUN
ajst-32578	31	32	into	into	ADP
ajst-32578	31	33	bl21(de3	bl21(de3	NOUN
ajst-32578	31	34	)	)	PUNCT
ajst-32578	31	35	,	,	PUNCT
ajst-32578	31	36	pick	pick	VERB
ajst-32578	31	37	single	single	ADJ
ajst-32578	31	38	colonies	colony	NOUN
ajst-32578	31	39	,	,	PUNCT
ajst-32578	31	40	and	and	CCONJ
ajst-32578	31	41	inoculate	inoculate	VERB
ajst-32578	31	42	them	they	PRON
ajst-32578	31	43	onto	onto	ADP
ajst-32578	31	44	kan	kan	NOUN
ajst-32578	31	45	-	-	PUNCT
ajst-32578	31	46	containing	contain	VERB
ajst-32578	31	47	lb	lb	DET
ajst-32578	31	48	medium	medium	NOUN
ajst-32578	31	49	for	for	ADP
ajst-32578	31	50	overnight	overnight	ADJ
ajst-32578	31	51	incubation	incubation	NOUN
ajst-32578	31	52	at	at	ADP
ajst-32578	31	53	37	37	NUM
ajst-32578	31	54	℃	℃	PROPN
ajst-32578	31	55	°	°	PROPN
ajst-32578	31	56	c	c	NOUN
ajst-32578	31	57	under	under	ADP
ajst-32578	31	58	shaking	shake	VERB
ajst-32578	31	59	;	;	PUNCT
ajst-32578	31	60	3	3	X
ajst-32578	31	61	)	)	PUNCT
ajst-32578	31	62	optimization	optimization	NOUN
ajst-32578	31	63	of	of	ADP
ajst-32578	31	64	induction	induction	NOUN
ajst-32578	31	65	conditions	condition	NOUN
ajst-32578	31	66	:	:	PUNCT
ajst-32578	31	67	subculture	subculture	NOUN
ajst-32578	31	68	to	to	ADP
ajst-32578	31	69	fresh	fresh	ADJ
ajst-32578	31	70	lb+kan	lb+kan	VERB
ajst-32578	31	71	medium	medium	NOUN
ajst-32578	31	72	at	at	ADP
ajst-32578	31	73	a	a	DET
ajst-32578	31	74	1:100	1:100	NUM
ajst-32578	31	75	dilution	dilution	NOUN
ajst-32578	31	76	ratio	ratio	NOUN
ajst-32578	31	77	,	,	PUNCT
ajst-32578	31	78	test	test	VERB
ajst-32578	31	79	different	different	ADJ
ajst-32578	31	80	iptg	iptg	NOUN
ajst-32578	31	81	concentrations	concentration	NOUN
ajst-32578	31	82	(	(	PUNCT
ajst-32578	31	83	0.1	0.1	NUM
ajst-32578	31	84	-	-	SYM
ajst-32578	31	85	1	1	NUM
ajst-32578	31	86	mm	mm	NOUN
ajst-32578	31	87	)	)	PUNCT
ajst-32578	31	88	,	,	PUNCT
ajst-32578	32	1	temperatures	temperature	NOUN
ajst-32578	32	2	(	(	PUNCT
ajst-32578	32	3	16	16	NUM
ajst-32578	32	4	℃	℃	PROPN
ajst-32578	32	5	,25	,25	PUNCT
ajst-32578	32	6	℃	℃	PROPN
ajst-32578	32	7	,37	,37	PUNCT
ajst-32578	32	8	℃	℃	PROPN
ajst-32578	32	9	°	°	PROPN
ajst-32578	32	10	c	c	NOUN
ajst-32578	32	11	)	)	PUNCT
ajst-32578	32	12	,	,	PUNCT
ajst-32578	32	13	and	and	CCONJ
ajst-32578	32	14	induction	induction	NOUN
ajst-32578	32	15	durations	duration	NOUN
ajst-32578	32	16	(	(	PUNCT
ajst-32578	32	17	4	4	NUM
ajst-32578	32	18	-	-	SYM
ajst-32578	32	19	16	16	NUM
ajst-32578	32	20	h	h	NOUN
ajst-32578	32	21	)	)	PUNCT
ajst-32578	32	22	,	,	PUNCT
ajst-32578	32	23	and	and	CCONJ
ajst-32578	32	24	screen	screen	NOUN
ajst-32578	32	25	for	for	ADP
ajst-32578	32	26	optimal	optimal	ADJ
ajst-32578	32	27	expression	expression	NOUN
ajst-32578	32	28	conditions	condition	NOUN
ajst-32578	32	29	through	through	ADP
ajst-32578	32	30	sds	sds	NOUN
ajst-32578	32	31	-	-	PUNCT
ajst-32578	32	32	page	page	NOUN
ajst-32578	32	33	analysis	analysis	NOUN
ajst-32578	32	34	.	.	PUNCT
ajst-32578	33	1	2.2.4	2.2.4	NUM
ajst-32578	33	2	protein	protein	NOUN
ajst-32578	33	3	purification	purification	NOUN
ajst-32578	33	4	1	1	NUM
ajst-32578	33	5	)	)	PUNCT
ajst-32578	33	6	bacterial	bacterial	ADJ
ajst-32578	33	7	cell	cell	NOUN
ajst-32578	33	8	disruption	disruption	NOUN
ajst-32578	33	9	:	:	PUNCT
ajst-32578	33	10	collect	collect	VERB
ajst-32578	33	11	the	the	DET
ajst-32578	33	12	bacterial	bacterial	ADJ
ajst-32578	33	13	suspension	suspension	NOUN
ajst-32578	33	14	cultured	cultured	ADJ
ajst-32578	33	15	under	under	ADP
ajst-32578	33	16	optimal	optimal	ADJ
ajst-32578	33	17	conditions	condition	NOUN
ajst-32578	33	18	by	by	ADP
ajst-32578	33	19	centrifugation	centrifugation	NOUN
ajst-32578	33	20	at	at	ADP
ajst-32578	33	21	4	4	NUM
ajst-32578	33	22	℃	℃	PROPN
ajst-32578	33	23	g.	g.	NOUN
ajst-32578	33	24	resuspend	resuspend	VERB
ajst-32578	33	25	in	in	ADP
ajst-32578	33	26	protease	protease	NOUN
ajst-32578	33	27	inhibitor	inhibitor	NOUN
ajst-32578	33	28	-	-	PUNCT
ajst-32578	33	29	containing	contain	VERB
ajst-32578	33	30	lysis	lysis	NOUN
ajst-32578	33	31	buffer	buffer	NOUN
ajst-32578	33	32	and	and	CCONJ
ajst-32578	33	33	perform	perform	VERB
ajst-32578	33	34	icecryoprecipitation	icecryoprecipitation	NOUN
ajst-32578	33	35	using	use	VERB
ajst-32578	33	36	sonication	sonication	NOUN
ajst-32578	33	37	(	(	PUNCT
ajst-32578	33	38	power	power	NOUN
ajst-32578	33	39	300w,3s	300w,3s	NUM
ajst-32578	33	40	on/5s	on/5s	ADV
ajst-32578	33	41	off	off	ADV
ajst-32578	33	42	,	,	PUNCT
ajst-32578	33	43	total	total	ADJ
ajst-32578	33	44	duration	duration	NOUN
ajst-32578	33	45	15	15	NUM
ajst-32578	33	46	-	-	SYM
ajst-32578	33	47	20	20	NUM
ajst-32578	33	48	minutes	minute	NOUN
ajst-32578	33	49	)	)	PUNCT
ajst-32578	33	50	.	.	PUNCT
ajst-32578	34	1	2	2	X
ajst-32578	34	2	)	)	PUNCT
ajst-32578	34	3	centrifugation	centrifugation	NOUN
ajst-32578	34	4	and	and	CCONJ
ajst-32578	34	5	separation	separation	NOUN
ajst-32578	34	6	:	:	PUNCT
ajst-32578	34	7	centrifuge	centrifuge	NOUN
ajst-32578	34	8	at	at	ADP
ajst-32578	34	9	4	4	NUM
ajst-32578	34	10	℃	℃	PROPN
ajst-32578	34	11	g	g	NOUN
ajst-32578	34	12	,	,	PUNCT
ajst-32578	34	13	12,000	12,000	NUM
ajst-32578	34	14	rpm	rpm	NOUN
ajst-32578	34	15	for	for	ADP
ajst-32578	34	16	30	30	NUM
ajst-32578	34	17	minutes	minute	NOUN
ajst-32578	34	18	to	to	PART
ajst-32578	34	19	collect	collect	VERB
ajst-32578	34	20	the	the	DET
ajst-32578	34	21	supernatant	supernatant	NOUN
ajst-32578	34	22	(	(	PUNCT
ajst-32578	34	23	containing	contain	VERB
ajst-32578	34	24	soluble	soluble	ADJ
ajst-32578	34	25	proteins	protein	NOUN
ajst-32578	34	26	)	)	PUNCT
ajst-32578	34	27	.	.	PUNCT
ajst-32578	35	1	3	3	X
ajst-32578	35	2	)	)	PUNCT
ajst-32578	35	3	affinity	affinity	NOUN
ajst-32578	35	4	chromatofigurey	chromatofigurey	PROPN
ajst-32578	35	5	:	:	PUNCT
ajst-32578	35	6	load	load	VERB
ajst-32578	35	7	the	the	DET
ajst-32578	35	8	storage	storage	NOUN
ajst-32578	35	9	buffer	buffer	NOUN
ajst-32578	35	10	through	through	ADP
ajst-32578	35	11	gravity	gravity	NOUN
ajst-32578	35	12	flow	flow	NOUN
ajst-32578	35	13	using	use	VERB
ajst-32578	35	14	a	a	DET
ajst-32578	35	15	ni	ni	PROPN
ajst-32578	35	16	-	-	PUNCT
ajst-32578	35	17	nta	nta	PROPN
ajst-32578	35	18	preloaded	preloaded	ADJ
ajst-32578	35	19	column	column	NOUN
ajst-32578	35	20	.	.	PUNCT
ajst-32578	36	1	equilibrate	equilibrate	VERB
ajst-32578	36	2	the	the	DET
ajst-32578	36	3	column	column	NOUN
ajst-32578	36	4	with	with	ADP
ajst-32578	36	5	twice	twice	DET
ajst-32578	36	6	its	its	PRON
ajst-32578	36	7	volume	volume	NOUN
ajst-32578	36	8	of	of	ADP
ajst-32578	36	9	binding	bind	VERB
ajst-32578	36	10	/	/	SYM
ajst-32578	36	11	wash	wash	NOUN
ajst-32578	36	12	buffer	buffer	NOUN
ajst-32578	36	13	at	at	ADP
ajst-32578	36	14	a	a	DET
ajst-32578	36	15	flow	flow	NOUN
ajst-32578	36	16	rate	rate	NOUN
ajst-32578	36	17	of	of	ADP
ajst-32578	36	18	0.5	0.5	NUM
ajst-32578	36	19	ml	ml	NOUN
ajst-32578	36	20	/	/	SYM
ajst-32578	36	21	min	min	NOUN
ajst-32578	36	22	to	to	PART
ajst-32578	36	23	allow	allow	VERB
ajst-32578	36	24	gradual	gradual	ADJ
ajst-32578	36	25	buffer	buffer	NOUN
ajst-32578	36	26	elution	elution	NOUN
ajst-32578	36	27	.	.	PUNCT
ajst-32578	37	1	prepare	prepare	VERB
ajst-32578	37	2	the	the	DET
ajst-32578	37	3	sample	sample	NOUN
ajst-32578	37	4	solution	solution	NOUN
ajst-32578	37	5	by	by	ADP
ajst-32578	37	6	mixing	mix	VERB
ajst-32578	37	7	protein	protein	NOUN
ajst-32578	37	8	extract	extract	NOUN
ajst-32578	37	9	with	with	ADP
ajst-32578	37	10	binding	bind	VERB
ajst-32578	37	11	/	/	SYM
ajst-32578	37	12	wash	wash	NOUN
ajst-32578	37	13	buffer	buffer	NOUN
ajst-32578	37	14	in	in	ADP
ajst-32578	37	15	a	a	DET
ajst-32578	37	16	1:1	1:1	NUM
ajst-32578	37	17	ratio	ratio	NOUN
ajst-32578	37	18	,	,	PUNCT
ajst-32578	37	19	ensuring	ensure	VERB
ajst-32578	37	20	the	the	DET
ajst-32578	37	21	total	total	ADJ
ajst-32578	37	22	volume	volume	NOUN
ajst-32578	37	23	is	be	AUX
ajst-32578	37	24	twice	twice	DET
ajst-32578	37	25	the	the	DET
ajst-32578	37	26	column	column	NOUN
ajst-32578	37	27	volume	volume	NOUN
ajst-32578	37	28	.	.	PUNCT
ajst-32578	38	1	load	load	VERB
ajst-32578	38	2	the	the	DET
ajst-32578	38	3	sample	sample	NOUN
ajst-32578	38	4	solution	solution	NOUN
ajst-32578	38	5	onto	onto	ADP
ajst-32578	38	6	the	the	DET
ajst-32578	38	7	column	column	NOUN
ajst-32578	38	8	and	and	CCONJ
ajst-32578	38	9	collect	collect	VERB
ajst-32578	38	10	the	the	DET
ajst-32578	38	11	eluate	eluate	NOUN
ajst-32578	38	12	into	into	ADP
ajst-32578	38	13	centrifuge	centrifuge	NOUN
ajst-32578	38	14	tubes	tube	NOUN
ajst-32578	38	15	.	.	PUNCT
ajst-32578	39	1	wash	wash	VERB
ajst-32578	39	2	the	the	DET
ajst-32578	39	3	column	column	NOUN
ajst-32578	39	4	with	with	ADP
ajst-32578	39	5	twice	twice	DET
ajst-32578	39	6	its	its	PRON
ajst-32578	39	7	volume	volume	NOUN
ajst-32578	39	8	of	of	ADP
ajst-32578	39	9	binding	bind	VERB
ajst-32578	39	10	/	/	SYM
ajst-32578	39	11	wash	wash	NOUN
ajst-32578	39	12	buffer	buffer	NOUN
ajst-32578	39	13	and	and	CCONJ
ajst-32578	39	14	collect	collect	VERB
ajst-32578	39	15	the	the	DET
ajst-32578	39	16	eluate	eluate	ADJ
ajst-32578	39	17	.	.	PUNCT
ajst-32578	40	1	elute	elute	VERB
ajst-32578	40	2	histidine	histidine	NOUN
ajst-32578	40	3	-	-	PUNCT
ajst-32578	40	4	tagged	tag	VERB
ajst-32578	40	5	proteins	protein	NOUN
ajst-32578	40	6	from	from	ADP
ajst-32578	40	7	the	the	DET
ajst-32578	40	8	column	column	NOUN
ajst-32578	40	9	using	use	VERB
ajst-32578	40	10	twice	twice	DET
ajst-32578	40	11	its	its	PRON
ajst-32578	40	12	volume	volume	NOUN
ajst-32578	40	13	of	of	ADP
ajst-32578	40	14	elution	elution	NOUN
ajst-32578	40	15	buffer	buffer	NOUN
ajst-32578	40	16	,	,	PUNCT
ajst-32578	40	17	repeating	repeat	VERB
ajst-32578	40	18	this	this	DET
ajst-32578	40	19	step	step	NOUN
ajst-32578	40	20	twice	twice	ADV
ajst-32578	40	21	.	.	PUNCT
ajst-32578	41	1	store	store	VERB
ajst-32578	41	2	each	each	DET
ajst-32578	41	3	eluate	eluate	ADJ
ajst-32578	41	4	separately	separately	ADV
ajst-32578	41	5	until	until	ADP
ajst-32578	41	6	absorbance	absorbance	NOUN
ajst-32578	41	7	at	at	ADP
ajst-32578	41	8	280	280	NUM
ajst-32578	41	9	nm	nm	PRON
ajst-32578	41	10	approaches	approach	NOUN
ajst-32578	41	11	baseline	baseline	NOUN
ajst-32578	41	12	levels	level	NOUN
ajst-32578	41	13	(	(	PUNCT
ajst-32578	41	14	as	as	SCONJ
ajst-32578	41	15	shown	show	VERB
ajst-32578	41	16	in	in	ADP
ajst-32578	41	17	figure	figure	NOUN
ajst-32578	41	18	2	2	NUM
ajst-32578	41	19	)	)	PUNCT
ajst-32578	41	20	.	.	PUNCT
ajst-32578	42	1	figure	figure	NOUN
ajst-32578	42	2	2	2	NUM
ajst-32578	42	3	.	.	X
ajst-32578	42	4	affinity	affinity	NOUN
ajst-32578	42	5	purification	purification	NOUN
ajst-32578	42	6	process	process	NOUN
ajst-32578	42	7	of	of	ADP
ajst-32578	42	8	recombinant	recombinant	ADJ
ajst-32578	42	9	protein	protein	NOUN
ajst-32578	42	10	vp1	vp1	NOUN
ajst-32578	42	11	2.2.5	2.2.5	NUM
ajst-32578	42	12	sds	sds	NOUN
ajst-32578	42	13	-	-	PUNCT
ajst-32578	42	14	page	page	NOUN
ajst-32578	42	15	:	:	PUNCT
ajst-32578	42	16	detection	detection	NOUN
ajst-32578	42	17	of	of	ADP
ajst-32578	42	18	molecular	molecular	ADJ
ajst-32578	42	19	weight	weight	NOUN
ajst-32578	42	20	and	and	CCONJ
ajst-32578	42	21	purity	purity	NOUN
ajst-32578	42	22	of	of	ADP
ajst-32578	42	23	purified	purified	ADJ
ajst-32578	42	24	protein	protein	NOUN
ajst-32578	42	25	;	;	PUNCT
ajst-32578	42	26	3	3	X
ajst-32578	42	27	.	.	NOUN
ajst-32578	42	28	results	result	NOUN
ajst-32578	42	29	and	and	CCONJ
ajst-32578	42	30	analysis	analysis	NOUN
ajst-32578	42	31	3.1	3.1	NUM
ajst-32578	42	32	construction	construction	NOUN
ajst-32578	42	33	of	of	ADP
ajst-32578	42	34	recombinant	recombinant	ADJ
ajst-32578	42	35	plasmids	plasmid	NOUN
ajst-32578	42	36	the	the	DET
ajst-32578	42	37	restriction	restriction	NOUN
ajst-32578	42	38	enzyme	enzyme	NOUN
ajst-32578	42	39	digestion	digestion	NOUN
ajst-32578	42	40	results	result	NOUN
ajst-32578	42	41	showed	show	VERB
ajst-32578	42	42	approximately	approximately	ADV
ajst-32578	42	43	2	2	NUM
ajst-32578	42	44	kb	kb	NOUN
ajst-32578	42	45	of	of	ADP
ajst-32578	42	46	vector	vector	NOUN
ajst-32578	42	47	fragments	fragment	NOUN
ajst-32578	42	48	and	and	CCONJ
ajst-32578	42	49	target	target	VERB
ajst-32578	42	50	gene	gene	NOUN
ajst-32578	42	51	fragments	fragment	NOUN
ajst-32578	42	52	(	(	PUNCT
ajst-32578	42	53	with	with	ADP
ajst-32578	42	54	expected	expect	VERB
ajst-32578	42	55	sizes	size	NOUN
ajst-32578	42	56	)	)	PUNCT
ajst-32578	42	57	after	after	ADP
ajst-32578	42	58	double	double	ADJ
ajst-32578	42	59	digestion	digestion	NOUN
ajst-32578	42	60	with	with	ADP
ajst-32578	42	61	ecorv	ecorv	NOUN
ajst-32578	42	62	and	and	CCONJ
ajst-32578	42	63	xhoi	xhoi	ADJ
ajst-32578	42	64	enzymes	enzyme	NOUN
ajst-32578	42	65	,	,	PUNCT
ajst-32578	42	66	confirming	confirm	VERB
ajst-32578	42	67	the	the	DET
ajst-32578	42	68	successful	successful	ADJ
ajst-32578	42	69	construction	construction	NOUN
ajst-32578	42	70	of	of	ADP
ajst-32578	42	71	the	the	DET
ajst-32578	42	72	recombinant	recombinant	ADJ
ajst-32578	42	73	plasmid	plasmid	NOUN
ajst-32578	42	74	(	(	PUNCT
ajst-32578	42	75	figure	figure	NOUN
ajst-32578	42	76	2	2	NUM
ajst-32578	42	77	)	)	PUNCT
ajst-32578	42	78	.	.	PUNCT
ajst-32578	43	1	genetic	genetic	ADJ
ajst-32578	43	2	sequencing	sequencing	NOUN
ajst-32578	43	3	revealed	reveal	VERB
ajst-32578	43	4	that	that	SCONJ
ajst-32578	43	5	the	the	DET
ajst-32578	43	6	insert	insert	ADJ
ajst-32578	43	7	sequences	sequence	NOUN
ajst-32578	43	8	of	of	ADP
ajst-32578	43	9	the	the	DET
ajst-32578	43	10	recombinant	recombinant	ADJ
ajst-32578	43	11	plasmid	plasmid	ADJ
ajst-32578	43	12	pet-32a	pet-32a	NOUN
ajst-32578	43	13	(	(	PUNCT
ajst-32578	43	14	+	+	NOUN
ajst-32578	43	15	)	)	PUNCT
ajst-32578	43	16	-vp1	-vp1	VERB
ajst-32578	43	17	matched	match	VERB
ajst-32578	43	18	the	the	DET
ajst-32578	43	19	expected	expect	VERB
ajst-32578	43	20	sequences	sequence	NOUN
ajst-32578	43	21	without	without	ADP
ajst-32578	43	22	any	any	DET
ajst-32578	43	23	mutations	mutation	NOUN
ajst-32578	43	24	or	or	CCONJ
ajst-32578	43	25	frameshifts	frameshift	NOUN
ajst-32578	43	26	(	(	PUNCT
ajst-32578	43	27	figure	figure	VERB
ajst-32578	43	28	3	3	NUM
ajst-32578	43	29	)	)	PUNCT
ajst-32578	43	30	.	.	PUNCT
ajst-32578	44	1	235	235	NUM
ajst-32578	44	2	figure	figure	NOUN
ajst-32578	44	3	3	3	NUM
ajst-32578	44	4	.	.	PUNCT
ajst-32578	44	5	electrophoresis	electrophoresis	NOUN
ajst-32578	44	6	results	result	NOUN
ajst-32578	44	7	of	of	ADP
ajst-32578	44	8	double	double	ADJ
ajst-32578	44	9	enzyme	enzyme	NOUN
ajst-32578	44	10	digestion	digestion	NOUN
ajst-32578	44	11	verification	verification	NOUN
ajst-32578	44	12	of	of	ADP
ajst-32578	44	13	bacterial	bacterial	ADJ
ajst-32578	44	14	plasmid	plasmid	ADJ
ajst-32578	44	15	extraction	extraction	NOUN
ajst-32578	44	16	lane	lane	NOUN
ajst-32578	44	17	m	m	PROPN
ajst-32578	44	18	:	:	PUNCT
ajst-32578	44	19	kb	kb	PROPN
ajst-32578	44	20	ladder	ladder	NOUN
ajst-32578	44	21	lane	lane	NOUN
ajst-32578	44	22	1	1	NUM
ajst-32578	44	23	:	:	PUNCT
ajst-32578	44	24	plasmid	plasmid	ADJ
ajst-32578	44	25	lane	lane	NOUN
ajst-32578	44	26	2	2	NUM
ajst-32578	44	27	:	:	PUNCT
ajst-32578	44	28	plasmid	plasmid	NOUN
ajst-32578	44	29	digested	digest	VERB
ajst-32578	44	30	by	by	ADP
ajst-32578	44	31	ecorv	ecorv	NOUN
ajst-32578	44	32	and	and	CCONJ
ajst-32578	44	33	xhoi	xhoi	ADJ
ajst-32578	44	34	figure	figure	NOUN
ajst-32578	44	35	4	4	NUM
ajst-32578	44	36	.	.	PUNCT
ajst-32578	44	37	chromosome	chromosome	NOUN
ajst-32578	44	38	sequencing	sequence	VERB
ajst-32578	44	39	report	report	NOUN
ajst-32578	44	40	3.2	3.2	NUM
ajst-32578	44	41	expression	expression	NOUN
ajst-32578	44	42	and	and	CCONJ
ajst-32578	44	43	purification	purification	NOUN
ajst-32578	44	44	of	of	ADP
ajst-32578	44	45	recombinant	recombinant	ADJ
ajst-32578	44	46	protein	protein	NOUN
ajst-32578	44	47	sds	sds	NOUN
ajst-32578	44	48	-	-	PUNCT
ajst-32578	44	49	page	page	NOUN
ajst-32578	44	50	analysis	analysis	NOUN
ajst-32578	44	51	revealed	reveal	VERB
ajst-32578	44	52	a	a	DET
ajst-32578	44	53	specific	specific	ADJ
ajst-32578	44	54	band	band	NOUN
ajst-32578	44	55	at	at	ADP
ajst-32578	44	56	approximately	approximately	ADV
ajst-32578	44	57	40	40	NUM
ajst-32578	44	58	kda	kda	NOUN
ajst-32578	44	59	following	follow	VERB
ajst-32578	44	60	iptg	iptg	PROPN
ajst-32578	44	61	induction	induction	NOUN
ajst-32578	44	62	,	,	PUNCT
ajst-32578	44	63	matching	match	VERB
ajst-32578	44	64	the	the	DET
ajst-32578	44	65	theoretical	theoretical	ADJ
ajst-32578	44	66	molecular	molecular	ADJ
ajst-32578	44	67	weight	weight	NOUN
ajst-32578	44	68	of	of	ADP
ajst-32578	44	69	vp1	vp1	NOUN
ajst-32578	44	70	fusion	fusion	NOUN
ajst-32578	44	71	protein	protein	NOUN
ajst-32578	44	72	.	.	PUNCT
ajst-32578	45	1	the	the	DET
ajst-32578	45	2	optimized	optimize	VERB
ajst-32578	45	3	expression	expression	NOUN
ajst-32578	45	4	236	236	NUM
ajst-32578	45	5	conditions	condition	NOUN
ajst-32578	45	6	were	be	AUX
ajst-32578	45	7	determined	determine	VERB
ajst-32578	45	8	as	as	ADP
ajst-32578	45	9	:	:	PUNCT
ajst-32578	45	10	final	final	ADJ
ajst-32578	45	11	iptg	iptg	NOUN
ajst-32578	45	12	concentration	concentration	NOUN
ajst-32578	45	13	0.5	0.5	NUM
ajst-32578	45	14	mm	mm	NOUN
ajst-32578	45	15	and	and	CCONJ
ajst-32578	45	16	16	16	NUM
ajst-32578	45	17	℃	℃	PROPN
ajst-32578	45	18	induction	induction	NOUN
ajst-32578	45	19	for	for	ADP
ajst-32578	45	20	16	16	NUM
ajst-32578	45	21	hours	hour	NOUN
ajst-32578	45	22	,	,	PUNCT
ajst-32578	45	23	achieving	achieve	VERB
ajst-32578	45	24	optimal	optimal	ADJ
ajst-32578	45	25	protein	protein	NOUN
ajst-32578	45	26	yield	yield	NOUN
ajst-32578	45	27	with	with	ADP
ajst-32578	45	28	high	high	ADJ
ajst-32578	45	29	solubility	solubility	NOUN
ajst-32578	45	30	.	.	PUNCT
ajst-32578	46	1	after	after	ADP
ajst-32578	46	2	ni	ni	PROPN
ajst-32578	46	3	-	-	PUNCT
ajst-32578	46	4	nta	nta	PROPN
ajst-32578	46	5	affinity	affinity	NOUN
ajst-32578	46	6	chromatofigurey	chromatofigurey	PROPN
ajst-32578	46	7	purification	purification	NOUN
ajst-32578	46	8	,	,	PUNCT
ajst-32578	46	9	the	the	DET
ajst-32578	46	10	sds	sds	NOUN
ajst-32578	46	11	-	-	PUNCT
ajst-32578	46	12	page	page	NOUN
ajst-32578	46	13	showed	show	VERB
ajst-32578	46	14	a	a	DET
ajst-32578	46	15	single	single	ADJ
ajst-32578	46	16	band	band	NOUN
ajst-32578	46	17	with	with	ADP
ajst-32578	46	18	over	over	ADP
ajst-32578	46	19	90	90	NUM
ajst-32578	46	20	%	%	NOUN
ajst-32578	46	21	purity	purity	NOUN
ajst-32578	46	22	,	,	PUNCT
ajst-32578	46	23	and	and	CCONJ
ajst-32578	46	24	the	the	DET
ajst-32578	46	25	protein	protein	NOUN
ajst-32578	46	26	concentration	concentration	NOUN
ajst-32578	46	27	was	be	AUX
ajst-32578	46	28	measured	measure	VERB
ajst-32578	46	29	at	at	ADP
ajst-32578	46	30	1.2	1.2	NUM
ajst-32578	46	31	mg	mg	NOUN
ajst-32578	46	32	/	/	SYM
ajst-32578	46	33	ml	ml	VERB
ajst-32578	46	34	using	use	VERB
ajst-32578	46	35	the	the	DET
ajst-32578	46	36	coomassie	coomassie	ADJ
ajst-32578	46	37	brilliant	brilliant	ADJ
ajst-32578	46	38	blue	blue	ADJ
ajst-32578	46	39	method	method	NOUN
ajst-32578	46	40	.	.	PUNCT
ajst-32578	47	1	figure	figure	NOUN
ajst-32578	47	2	5	5	NUM
ajst-32578	47	3	.	.	PUNCT
ajst-32578	47	4	sds	sds	NOUN
ajst-32578	47	5	-	-	PUNCT
ajst-32578	47	6	page	page	NOUN
ajst-32578	47	7	protein	protein	NOUN
ajst-32578	47	8	analysis	analysis	NOUN
ajst-32578	47	9	after	after	ADP
ajst-32578	47	10	ultrasonication	ultrasonication	NOUN
ajst-32578	47	11	and	and	CCONJ
ajst-32578	47	12	purification	purification	NOUN
ajst-32578	47	13	of	of	ADP
ajst-32578	47	14	iptg	iptg	NOUN
ajst-32578	47	15	-	-	PUNCT
ajst-32578	47	16	induced	induce	VERB
ajst-32578	47	17	bl21(de3	bl21(de3	NOUN
ajst-32578	47	18	)	)	PUNCT
ajst-32578	47	19	bacterial	bacterial	ADJ
ajst-32578	47	20	cells	cell	NOUN
ajst-32578	47	21	containing	contain	VERB
ajst-32578	47	22	the	the	DET
ajst-32578	47	23	recombinant	recombinant	ADJ
ajst-32578	47	24	plasmid	plasmid	NOUN
ajst-32578	47	25	,	,	PUNCT
ajst-32578	47	26	the	the	DET
ajst-32578	47	27	sds	sds	NOUN
ajst-32578	47	28	-	-	PUNCT
ajst-32578	47	29	page	page	NOUN
ajst-32578	47	30	results	result	NOUN
ajst-32578	47	31	showed	show	VERB
ajst-32578	47	32	a	a	DET
ajst-32578	47	33	specific	specific	ADJ
ajst-32578	47	34	band	band	NOUN
ajst-32578	47	35	at	at	ADP
ajst-32578	47	36	molecular	molecular	ADJ
ajst-32578	47	37	weight	weight	NOUN
ajst-32578	47	38	43	43	NUM
ajst-32578	47	39	kda	kda	NOUN
ajst-32578	47	40	(	(	PUNCT
ajst-32578	47	41	figure	figure	NOUN
ajst-32578	47	42	5	5	NUM
ajst-32578	47	43	)	)	PUNCT
ajst-32578	47	44	,	,	PUNCT
ajst-32578	47	45	matching	match	VERB
ajst-32578	47	46	the	the	DET
ajst-32578	47	47	theoretical	theoretical	ADJ
ajst-32578	47	48	molecular	molecular	ADJ
ajst-32578	47	49	weight	weight	NOUN
ajst-32578	47	50	.	.	PUNCT
ajst-32578	48	1	following	follow	VERB
ajst-32578	48	2	ni	ni	PROPN
ajst-32578	48	3	-	-	PUNCT
ajst-32578	48	4	nta	nta	PROPN
ajst-32578	48	5	purification	purification	NOUN
ajst-32578	48	6	,	,	PUNCT
ajst-32578	48	7	the	the	DET
ajst-32578	48	8	protein	protein	NOUN
ajst-32578	48	9	purity	purity	NOUN
ajst-32578	48	10	exceeded	exceed	VERB
ajst-32578	48	11	90	90	NUM
ajst-32578	48	12	%	%	NOUN
ajst-32578	48	13	(	(	PUNCT
ajst-32578	48	14	figure	figure	NOUN
ajst-32578	48	15	5	5	NUM
ajst-32578	48	16	)	)	PUNCT
ajst-32578	48	17	.	.	PUNCT
ajst-32578	49	1	4	4	X
ajst-32578	49	2	.	.	X
ajst-32578	49	3	conclusion	conclusion	NOUN
ajst-32578	49	4	through	through	ADP
ajst-32578	49	5	the	the	DET
ajst-32578	49	6	construction	construction	NOUN
ajst-32578	49	7	of	of	ADP
ajst-32578	49	8	pet-32a	pet-32a	NOUN
ajst-32578	49	9	(	(	PUNCT
ajst-32578	49	10	+	+	ADJ
ajst-32578	49	11	)	)	PUNCT
ajst-32578	49	12	-vip1	-vip1	NOUN
ajst-32578	49	13	recombinant	recombinant	ADJ
ajst-32578	49	14	plasmid	plasmid	NOUN
ajst-32578	49	15	and	and	CCONJ
ajst-32578	49	16	optimization	optimization	NOUN
ajst-32578	49	17	of	of	ADP
ajst-32578	49	18	expression	expression	NOUN
ajst-32578	49	19	conditions	condition	NOUN
ajst-32578	49	20	,	,	PUNCT
ajst-32578	49	21	this	this	DET
ajst-32578	49	22	study	study	NOUN
ajst-32578	49	23	achieved	achieve	VERB
ajst-32578	49	24	the	the	DET
ajst-32578	49	25	high	high	ADJ
ajst-32578	49	26	efficiency	efficiency	NOUN
ajst-32578	49	27	of	of	ADP
ajst-32578	49	28	pv1	pv1	NOUN
ajst-32578	49	29	vp1	vp1	NOUN
ajst-32578	49	30	protein	protein	NOUN
ajst-32578	49	31	expression	expression	NOUN
ajst-32578	49	32	in	in	ADP
ajst-32578	49	33	escherichia	escherichia	NOUN
ajst-32578	49	34	coli	coli	NOUN
ajst-32578	49	35	(	(	PUNCT
ajst-32578	49	36	43	43	NUM
ajst-32578	49	37	kda	kda	NOUN
ajst-32578	49	38	)	)	PUNCT
ajst-32578	49	39	with	with	ADP
ajst-32578	49	40	purity>90	purity>90	PROPN
ajst-32578	49	41	%	%	NOUN
ajst-32578	49	42	.	.	PUNCT
ajst-32578	50	1	this	this	DET
ajst-32578	50	2	system	system	NOUN
ajst-32578	50	3	laid	lay	VERB
ajst-32578	50	4	a	a	DET
ajst-32578	50	5	technical	technical	ADJ
ajst-32578	50	6	and	and	CCONJ
ajst-32578	50	7	material	material	NOUN
ajst-32578	50	8	foundation	foundation	NOUN
ajst-32578	50	9	for	for	ADP
ajst-32578	50	10	the	the	DET
ajst-32578	50	11	development	development	NOUN
ajst-32578	50	12	of	of	ADP
ajst-32578	50	13	polio	polio	NOUN
ajst-32578	50	14	subunit	subunit	NOUN
ajst-32578	50	15	vaccine	vaccine	NOUN
ajst-32578	50	16	.	.	PUNCT
ajst-32578	51	1	from	from	ADP
ajst-32578	51	2	a	a	DET
ajst-32578	51	3	technical	technical	ADJ
ajst-32578	51	4	perspective	perspective	NOUN
ajst-32578	51	5	,	,	PUNCT
ajst-32578	51	6	the	the	DET
ajst-32578	51	7	t7	t7	PROPN
ajst-32578	51	8	promoter	promoter	NOUN
ajst-32578	51	9	system	system	NOUN
ajst-32578	51	10	of	of	ADP
ajst-32578	51	11	pet-32a	pet-32a	NOUN
ajst-32578	51	12	(	(	PUNCT
ajst-32578	51	13	+	+	ADJ
ajst-32578	51	14	)	)	PUNCT
ajst-32578	51	15	vector	vector	NOUN
ajst-32578	51	16	can	can	AUX
ajst-32578	51	17	efficiently	efficiently	ADV
ajst-32578	51	18	induce	induce	VERB
ajst-32578	51	19	protein	protein	NOUN
ajst-32578	51	20	expression	expression	NOUN
ajst-32578	51	21	,	,	PUNCT
ajst-32578	51	22	and	and	CCONJ
ajst-32578	51	23	the	the	DET
ajst-32578	51	24	n	n	CCONJ
ajst-32578	51	25	-	-	PUNCT
ajst-32578	51	26	terminal	terminal	ADJ
ajst-32578	51	27	and	and	CCONJ
ajst-32578	51	28	c	c	NOUN
ajst-32578	51	29	-	-	PUNCT
ajst-32578	51	30	terminal	terminal	ADJ
ajst-32578	51	31	labeling	labeling	NOUN
ajst-32578	51	32	sequences	sequence	NOUN
ajst-32578	51	33	simplify	simplify	VERB
ajst-32578	51	34	the	the	DET
ajst-32578	51	35	process	process	NOUN
ajst-32578	51	36	of	of	ADP
ajst-32578	51	37	protein	protein	NOUN
ajst-32578	51	38	purification	purification	NOUN
ajst-32578	51	39	and	and	CCONJ
ajst-32578	51	40	identification	identification	NOUN
ajst-32578	51	41	.	.	PUNCT
ajst-32578	52	1	the	the	DET
ajst-32578	52	2	optimization	optimization	NOUN
ajst-32578	52	3	of	of	ADP
ajst-32578	52	4	induction	induction	NOUN
ajst-32578	52	5	conditions	condition	NOUN
ajst-32578	52	6	(	(	PUNCT
ajst-32578	52	7	such	such	ADJ
ajst-32578	52	8	as	as	ADP
ajst-32578	52	9	prolonging	prolong	VERB
ajst-32578	52	10	the	the	DET
ajst-32578	52	11	induction	induction	NOUN
ajst-32578	52	12	time	time	NOUN
ajst-32578	52	13	at	at	ADP
ajst-32578	52	14	low	low	ADJ
ajst-32578	52	15	temperature	temperature	NOUN
ajst-32578	52	16	)	)	PUNCT
ajst-32578	52	17	effectively	effectively	ADV
ajst-32578	52	18	improves	improve	VERB
ajst-32578	52	19	the	the	DET
ajst-32578	52	20	solubility	solubility	NOUN
ajst-32578	52	21	of	of	ADP
ajst-32578	52	22	protein	protein	NOUN
ajst-32578	52	23	and	and	CCONJ
ajst-32578	52	24	reduces	reduce	VERB
ajst-32578	52	25	the	the	DET
ajst-32578	52	26	formation	formation	NOUN
ajst-32578	52	27	of	of	ADP
ajst-32578	52	28	inclusions	inclusion	NOUN
ajst-32578	52	29	.	.	PUNCT
ajst-32578	53	1	compared	compare	VERB
ajst-32578	53	2	with	with	ADP
ajst-32578	53	3	existing	exist	VERB
ajst-32578	53	4	studies	study	NOUN
ajst-32578	53	5	,	,	PUNCT
ajst-32578	53	6	this	this	DET
ajst-32578	53	7	research	research	NOUN
ajst-32578	53	8	focuses	focus	VERB
ajst-32578	53	9	on	on	ADP
ajst-32578	53	10	key	key	ADJ
ajst-32578	53	11	epitopes	epitope	NOUN
ajst-32578	53	12	of	of	ADP
ajst-32578	53	13	vp1	vp1	NOUN
ajst-32578	53	14	protein	protein	NOUN
ajst-32578	53	15	rather	rather	ADV
ajst-32578	53	16	than	than	ADP
ajst-32578	53	17	complete	complete	ADJ
ajst-32578	53	18	viral	viral	ADJ
ajst-32578	53	19	particles	particle	NOUN
ajst-32578	53	20	,	,	PUNCT
ajst-32578	53	21	which	which	PRON
ajst-32578	53	22	reduces	reduce	VERB
ajst-32578	53	23	the	the	DET
ajst-32578	53	24	risk	risk	NOUN
ajst-32578	53	25	of	of	ADP
ajst-32578	53	26	nonspecific	nonspecific	ADJ
ajst-32578	53	27	reactions	reaction	NOUN
ajst-32578	53	28	and	and	CCONJ
ajst-32578	53	29	avoids	avoid	VERB
ajst-32578	53	30	the	the	DET
ajst-32578	53	31	vdpv	vdpv	NOUN
ajst-32578	53	32	risks	risk	NOUN
ajst-32578	53	33	associated	associate	VERB
ajst-32578	53	34	with	with	ADP
ajst-32578	53	35	oral	oral	ADJ
ajst-32578	53	36	poliovirus	poliovirus	NOUN
ajst-32578	53	37	vaccine	vaccine	NOUN
ajst-32578	53	38	(	(	PUNCT
ajst-32578	53	39	opv	opv	PROPN
ajst-32578	53	40	)	)	PUNCT
ajst-32578	53	41	and	and	CCONJ
ajst-32578	53	42	production	production	NOUN
ajst-32578	53	43	limitations	limitation	NOUN
ajst-32578	53	44	of	of	ADP
ajst-32578	53	45	inactivated	inactivated	ADJ
ajst-32578	53	46	poliovirus	poliovirus	NOUN
ajst-32578	53	47	vaccine	vaccine	NOUN
ajst-32578	53	48	(	(	PUNCT
ajst-32578	53	49	ipv	ipv	PROPN
ajst-32578	53	50	)	)	PUNCT
ajst-32578	53	51	.	.	PUNCT
ajst-32578	54	1	however	however	ADV
ajst-32578	54	2	,	,	PUNCT
ajst-32578	54	3	it	it	PRON
ajst-32578	54	4	should	should	AUX
ajst-32578	54	5	be	be	AUX
ajst-32578	54	6	noted	note	VERB
ajst-32578	54	7	that	that	SCONJ
ajst-32578	54	8	proteins	protein	NOUN
ajst-32578	54	9	expressed	express	VERB
ajst-32578	54	10	in	in	ADP
ajst-32578	54	11	escherichia	escherichia	NOUN
ajst-32578	54	12	coli	coli	NOUN
ajst-32578	54	13	lack	lack	NOUN
ajst-32578	54	14	glycosylation	glycosylation	NOUN
ajst-32578	54	15	modifications	modification	NOUN
ajst-32578	54	16	found	find	VERB
ajst-32578	54	17	in	in	ADP
ajst-32578	54	18	eukaryotic	eukaryotic	ADJ
ajst-32578	54	19	cells	cell	NOUN
ajst-32578	54	20	,	,	PUNCT
ajst-32578	54	21	potentially	potentially	ADV
ajst-32578	54	22	affecting	affect	VERB
ajst-32578	54	23	their	their	PRON
ajst-32578	54	24	immunogenicity	immunogenicity	NOUN
ajst-32578	54	25	.	.	PUNCT
ajst-32578	55	1	subsequent	subsequent	ADJ
ajst-32578	55	2	animal	animal	NOUN
ajst-32578	55	3	experiments	experiment	NOUN
ajst-32578	55	4	are	be	AUX
ajst-32578	55	5	required	require	VERB
ajst-32578	55	6	to	to	PART
ajst-32578	55	7	verify	verify	VERB
ajst-32578	55	8	their	their	PRON
ajst-32578	55	9	ability	ability	NOUN
ajst-32578	55	10	to	to	PART
ajst-32578	55	11	induce	induce	VERB
ajst-32578	55	12	neutralizing	neutralize	VERB
ajst-32578	55	13	antibodies	antibody	NOUN
ajst-32578	55	14	.	.	PUNCT
ajst-32578	56	1	references	reference	NOUN
ajst-32578	56	2	[	[	X
ajst-32578	56	3	1	1	X
ajst-32578	56	4	]	]	PUNCT
ajst-32578	56	5	yang	yang	PROPN
ajst-32578	56	6	xiaozhou	xiaozhou	PROPN
ajst-32578	56	7	et	et	PROPN
ajst-32578	56	8	al	al	PROPN
ajst-32578	56	9	.	.	PUNCT
ajst-32578	57	1	immunological	immunological	ADJ
ajst-32578	57	2	study	study	NOUN
ajst-32578	57	3	of	of	ADP
ajst-32578	57	4	anti	anti	ADJ
ajst-32578	57	5	-	-	ADJ
ajst-32578	57	6	surface	surface	ADJ
ajst-32578	57	7	epitope	epitope	NOUN
ajst-32578	57	8	chimeric	chimeric	ADJ
ajst-32578	57	9	proteins	protein	NOUN
ajst-32578	57	10	against	against	ADP
ajst-32578	57	11	type	type	NOUN
ajst-32578	57	12	iii	iii	NUM
ajst-32578	57	13	poliovirus	poliovirus	NOUN
ajst-32578	57	14	.	.	PUNCT
ajst-32578	58	1	[	[	X
ajst-32578	58	2	2	2	NUM
ajst-32578	58	3	]	]	PUNCT
ajst-32578	58	4	china	china	PROPN
ajst-32578	58	5	center	center	PROPN
ajst-32578	58	6	for	for	ADP
ajst-32578	58	7	disease	disease	NOUN
ajst-32578	58	8	control	control	NOUN
ajst-32578	58	9	and	and	CCONJ
ajst-32578	58	10	prevention	prevention	NOUN
ajst-32578	58	11	.	.	PUNCT
ajst-32578	59	1	technical	technical	ADJ
ajst-32578	59	2	guidelines	guideline	NOUN
ajst-32578	59	3	for	for	ADP
ajst-32578	59	4	polio	polio	NOUN
ajst-32578	59	5	prevention	prevention	NOUN
ajst-32578	59	6	and	and	CCONJ
ajst-32578	59	7	control	control	NOUN
ajst-32578	59	8	(	(	PUNCT
ajst-32578	59	9	2021	2021	NUM
ajst-32578	59	10	edition	edition	NOUN
ajst-32578	59	11	)	)	PUNCT
ajst-32578	60	1	[	[	X
ajst-32578	60	2	s	s	X
ajst-32578	60	3	]	]	PUNCT
ajst-32578	60	4	.	.	PUNCT
ajst-32578	61	1	peoples	people	NOUN
ajst-32578	61	2	medical	medical	ADJ
ajst-32578	61	3	publishing	publishing	PROPN
ajst-32578	61	4	house	house	PROPN
ajst-32578	61	5	,	,	PUNCT
ajst-32578	61	6	2021	2021	NUM
ajst-32578	61	7	.	.	PUNCT
ajst-32578	62	1	237	237	NUM
ajst-32578	63	1	[	[	SYM
ajst-32578	63	2	3	3	NUM
ajst-32578	63	3	]	]	X
ajst-32578	63	4	zhu	zhu	PROPN
ajst-32578	63	5	shuangli	shuangli	NOUN
ajst-32578	63	6	,	,	PUNCT
ajst-32578	63	7	yan	yan	PROPN
ajst-32578	63	8	dongmei	dongmei	PROPN
ajst-32578	64	1	et	et	PROPN
ajst-32578	64	2	al	al	PROPN
ajst-32578	64	3	.	.	PROPN
ajst-32578	64	4	surveillance	surveillance	NOUN
ajst-32578	64	5	and	and	CCONJ
ajst-32578	64	6	research	research	NOUN
ajst-32578	64	7	progress	progress	NOUN
ajst-32578	64	8	of	of	ADP
ajst-32578	64	9	poliovirus	poliovirus	NOUN
ajst-32578	64	10	vaccine	vaccine	NOUN
ajst-32578	64	11	-	-	PUNCT
ajst-32578	64	12	derived	derive	VERB
ajst-32578	64	13	viruses	virus	NOUN
ajst-32578	65	1	[	[	X
ajst-32578	65	2	j	j	X
ajst-32578	65	3	]	]	X
ajst-32578	65	4	.	.	PUNCT
ajst-32578	66	1	chinese	chinese	ADJ
ajst-32578	66	2	journal	journal	PROPN
ajst-32578	66	3	of	of	ADP
ajst-32578	66	4	preventive	preventive	ADJ
ajst-32578	66	5	medicine	medicine	NOUN
ajst-32578	66	6	,	,	PUNCT
ajst-32578	66	7	2023,57	2023,57	NUM
ajst-32578	66	8	(	(	PUNCT
ajst-32578	66	9	10	10	NUM
ajst-32578	66	10	):	):	PUNCT
ajst-32578	66	11	1414	1414	NUM
ajst-32578	66	12	-	-	SYM
ajst-32578	66	13	1419	1419	NUM
ajst-32578	66	14	.	.	PUNCT
ajst-32578	67	1	[	[	X
ajst-32578	67	2	4	4	NUM
ajst-32578	67	3	]	]	X
ajst-32578	67	4	yang	yang	PROPN
ajst-32578	67	5	zhanqiu	zhanqiu	PROPN
ajst-32578	67	6	,	,	PUNCT
ajst-32578	67	7	xiao	xiao	PROPN
ajst-32578	67	8	hong	hong	PROPN
ajst-32578	67	9	.	.	PUNCT
ajst-32578	68	1	molecular	molecular	ADJ
ajst-32578	68	2	virology	virology	NOUN
ajst-32578	68	3	of	of	ADP
ajst-32578	68	4	enterovirus	enterovirus	NOUN
ajst-32578	69	1	[	[	X
ajst-32578	70	1	m	m	X
ajst-32578	70	2	]	]	X
ajst-32578	70	3	.	.	PUNCT
ajst-32578	71	1	wuhan	wuhan	PROPN
ajst-32578	71	2	university	university	PROPN
ajst-32578	71	3	press	press	NOUN
ajst-32578	71	4	,	,	PUNCT
ajst-32578	71	5	2018	2018	NUM
ajst-32578	71	6	.	.	PUNCT
ajst-32578	72	1	[	[	X
ajst-32578	72	2	5	5	X
ajst-32578	72	3	]	]	X
ajst-32578	72	4	chen	chen	PROPN
ajst-32578	73	1	z	z	PROPN
ajst-32578	73	2	,	,	PUNCT
ajst-32578	73	3	et	et	PROPN
ajst-32578	73	4	al	al	PROPN
ajst-32578	73	5	.	.	PROPN
ajst-32578	74	1	structural	structural	ADJ
ajst-32578	74	2	and	and	CCONJ
ajst-32578	74	3	immunogenic	immunogenic	ADJ
ajst-32578	74	4	analysis	analysis	NOUN
ajst-32578	74	5	of	of	ADP
ajst-32578	74	6	poliovirus	poliovirus	NOUN
ajst-32578	74	7	vp1	vp1	NOUN
ajst-32578	74	8	protein	protein	NOUN
ajst-32578	74	9	.	.	PUNCT
ajst-32578	75	1	virology	virology	NOUN
ajst-32578	75	2	.2021;553:94	.2021;553:94	PROPN
ajst-32578	75	3	–	–	PUNCT
ajst-32578	75	4	102	102	NUM
ajst-32578	75	5	.	.	PUNCT
ajst-32578	76	1	[	[	X
ajst-32578	76	2	6	6	NUM
ajst-32578	76	3	]	]	X
ajst-32578	76	4	smith	smith	PROPN
ajst-32578	76	5	jl	jl	PROPN
ajst-32578	76	6	,	,	PUNCT
ajst-32578	76	7	et	et	PROPN
ajst-32578	76	8	al	al	PROPN
ajst-32578	76	9	.	.	PUNCT
ajst-32578	76	10	prokaryotic	prokaryotic	ADJ
ajst-32578	76	11	expression	expression	NOUN
ajst-32578	76	12	of	of	ADP
ajst-32578	76	13	viral	viral	ADJ
ajst-32578	76	14	antigens	antigen	NOUN
ajst-32578	76	15	for	for	ADP
ajst-32578	76	16	vaccine	vaccine	NOUN
ajst-32578	76	17	development	development	NOUN
ajst-32578	76	18	.	.	PUNCT
ajst-32578	77	1	vaccine	vaccine	NOUN
ajst-32578	77	2	.	.	PUNCT
ajst-32578	78	1	2019	2019	NUM
ajst-32578	78	2	;	;	PUNCT
ajst-32578	78	3	37	37	NUM
ajst-32578	78	4	(	(	PUNCT
ajst-32578	78	5	30	30	NUM
ajst-32578	78	6	):	):	PUNCT
ajst-32578	78	7	4023–4032	4023–4032	NUM
ajst-32578	78	8	.	.	PUNCT
ajst-32578	79	1	[	[	X
ajst-32578	79	2	7	7	X
ajst-32578	79	3	]	]	PUNCT
ajst-32578	79	4	world	world	NOUN
ajst-32578	79	5	health	health	NOUN
ajst-32578	79	6	organization	organization	NOUN
ajst-32578	79	7	.	.	PUNCT
ajst-32578	80	1	polio	polio	VERB
ajst-32578	80	2	vaccines	vaccine	NOUN
ajst-32578	80	3	:	:	PUNCT
ajst-32578	80	4	who	who	PRON
ajst-32578	80	5	position	position	VERB
ajst-32578	80	6	paper	paper	NOUN
ajst-32578	80	7	.	.	PUNCT
ajst-32578	81	1	wkly	wkly	ADJ
ajst-32578	81	2	epidemiol	epidemiol	PROPN
ajst-32578	81	3	rec	rec	PROPN
ajst-32578	81	4	.	.	PUNCT
ajst-32578	82	1	2022;97	2022;97	NUM
ajst-32578	82	2	(	(	PUNCT
ajst-32578	82	3	25	25	NUM
ajst-32578	82	4	):	):	PUNCT
ajst-32578	82	5	277–300	277–300	NUM
ajst-32578	82	6	.	.	PUNCT