56 ACTA BOT. CROAT. 80 (1), 2021 Acta Bot. Croat. 80 (1), 56–62, 2021 CODEN: ABCRA 25 DOI: 10.37427/botcro-2021-003 ISSN 0365-0588 eISSN 1847-8476 Ornithogalum sibthorpii Greuter (Asparagaceae), a species overlooked in Croatia Milica Rat1, Sandro Bogdanović2,3* 1 University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Trg Dositeja Obradovića 2, 21000 Novi Sad, Serbia 2 University of Zagreb, Faculty of Agriculture, Department of Agricultural Botany, Svetošimunska cesta 25, 10000 Zagreb, Croatia 3 Centre of Excellence for Biodiversity and Molecular Plant Breeding, Svetošimunska cesta 25, 10000 Zagreb, Croatia Abstract – Ornithogalum sibthorpii (Asparagaceae) is an early flowering species, with populations scattered across the Balkan Peninsula and Turkey. It inhabits rocky places and clearings, open habitats, parks and marginal parts of wet- lands. Based on the known distribution, habitat preferences and literature records for the Balkan Peninsula, it was hypothesised that this species might be distributed in Croatia as well. To confirm this, herbarium material was revised, and field investigations were organized. The first report confirmed that O. sibthorpii is widespread along the eastern Adriatic coast, reaching the inland Dinaric region too. To present the currently known localities in Croatia, a distribu- tion map is provided. Detailed morphological and leaf anatomy descriptions are given. Morphological affinities with similar species, O. excapum and O. refractum, are also briefly discussed, and an identification key is given. All Croatian populations of O. sibthorpii proved to be diploids with chromosome number 2n = 18. Keywords: anatomy, geophytes, karyology, morphology, Ornithogalum Introduction The genus Ornithogalum L. is characterized by a wide ecological tolerance and pronounced morphological vari- ability. According to Stevens (2001) it is one of the largest genera in the Asparagaceae family, with approximately 160 species (300 taxa). It is distributed in Africa, Europe and Southwest Asia (Speta 1998, Martínez-Azorin et al. 2013). Global databases record nearly 120 species with distribu- tions in the Mediterranean region and in the rest of Europe (Govaerts 2019, Euro+Med 2006–2019). According to Nikolić (2019a, b) in the Croatian flora there are 19 Orni­ thogalum species, three stenoendemic, and 10 species be- longing to the subgenus Ornithogalum. Several discrete European Ornithogalum groups are dif- ferentiated based on flowering time, that is, species flower- ing in late winter/early spring, spring, and in late spring/ early summer. One of the species that flower in late winter/ early spring is Ornithogalum sibthorpii Greuter. It is a rare and scattered species, distributed in the western part of Tur- key and in the Balkan Peninsula, in the north reaching Do- brugea in Romania (Sibthorp and Smith 1809, Baker 1873a, b, Markgraf 1932, Radenkova 1964, Zahariadi 1966, 1977, 1980, Diklić 1975, Landström 1989, Speta 1990, Rat and Ba- rina 2017, Rat 2019). It prefers karst, rocky ground, clearings and open habitats (Zahariadi 1980), and belongs to the group of Ornithogalum species that have underground scape, bearing from one to many flowers on the shortened inflorescence. Taxonomically significant characters com- pared with morphologically similar species in the investi- gated region (O. exscapum Ten. and O. refractum Kit. ex Schltdl.) are the more or less pronounced pulvinus, refract- ed pedicels at anthesis and bulbs without bulbils (Zahariadi 1980, Landström 1989). There is one literature reference indicating that O. sib­ thorpii is distributed in Croatia. Ascherson and Graebner (1905–1907: 249) stated that Ornithogalum nanum Smith in Sibth. et Smith (today synonym of O. sibthorpii) is recorded * Corresponding author e-mail: sbogdanovic@agr.hr ORNITHOGALUM SIBTHORPII IN CROATIA ACTA BOT. CROAT. 80 (1), 2021 57 1997). Image analysis and measurements of chromosomes were completed using KaryoType 2 software (Altinordu et al. 2016). Results Ornithogalum sibthorpii Greuter, Boissiera 13: 160 (1967) (Fig. 1) Type – “In Arcadia, et prope Abydum, Martio florens”, Smith loc. cit. (OXF!; file name: Sib-0793). Synonyms – Ornithogalum nanum Smith in Sibth. et Smith, Fl. Graec. Prodr. 1: 230 (1809); Fl. Graec. (Sibthorp). 4: 28, t. 333 (1823); non O. nanum (Burm. f.) Thunb., Prodr. Fl. Cap. 62 (1794). Morphological description (based on Croatian material, Fig. 1) ‒ Bulb hypogeal, ovoid, 12 – 15 × 0.8 – 20 mm, with- in Ljubuški and Stolac in Bosnia and Herzegovina, near to the Croatian border. Furthermore, for general distribution they cited the Adriatic region which covers an important part of the Croatian coast. For that reason, herbarium revi- sions and field trips were organized, with the aim of provid- ing new data about the potential distribution of the species in the western part of the Balkan Peninsula i.e. Croatia, and to complement it with biological and ecological descriptions. Materials and methods Field trips were organized during season in 2015 and 2016. Plant material was collected for morphological, ana- tomical and karyological analysis (Tab. 1). Voucher speci- mens are kept in the herbarium collections ZAGR and BUNS. Herbarium materials of O. sibthorpii were revised in BEO, BEOU, BP, BUNS, K, MKNH, SOM, SARA, W, WU, ZA, ZAGR and ZAHO. Virtual collections G, GZU and OXF were accessed as well. Herbaria acronyms follow Thiers (2019). A distribution map of O. sibthorpii in Croatia was produced in QGIS software ver. 3.10. Observations and morphological analyses included qualitative and quantitative description of bulb, leaves, scape and inflorescence with flowers. Ten fresh plants were collected and pressed for analysis. All observations were performed on fresh material using a Leica M205C binocu- lar stereomicroscope, while measurements were carried out using Digimizer image analysis software ver. 4.2.6.0. Anatomical investigations included a description of leaf structure. Cross sections were cut from the middle part of the leaf blade, using Leica CM 1850 cryostat, at –20 ºC with a cutting interval of 60 ηm. Observations and measure- ments were performed using a ZEISS light microscope Ax- ioVision A2, equipped with a ProgRes Speed XTCore5 camera and CapturePro v.2.8.8 image analysis software. For chromosome counting and analyses, 10 bulbs were planted in pots, and young root tips were collected, treated with 0.5% colchicine solution for 1 h at room temperature, and then fixed with a fresh solution of ethanol and glacial acid (3:1) for storage. To visualize metaphase chromosomes, root tips were hydrolysed with 1M HCl at room tempera- ture, and then stained with Schiff ’s reagent. Standard squash technique was used for preparation of slides (Jong Tab. 1. List of examined plant materials of Ornithogalum sibthorpii in Croatia. Locality Latitude / Longitude Collecting date Collector(s) Herbarium voucher number Croatia, Brgat (South Dalmatia) 42°38’45.06” 18°09’30.63” 28.03.2015 M. Rat BUNS-2-1151 Croatia, Nin (North Dalmatia) 44°14’48.58” 15°10’38.10” 23.04.2016 S. Bogdanović, V. Šegota, Z. Ljubešić ZAGR-41135 Croatia, Donji Lapac (Lika) 44°31’36.82” 15°58’44.48” 06.05.2016 S. Bogdanović ZAGR-55520 ZAGR-55521 Croatia, Sukošan (North Dalmatia) 44°02’59.10” 15°24’17.05” 05.04.2018 M. Pandža, M. Milović ZAGR-46301 Fig. 1. Ornithogalum sibthorpii (Croatia, material from Brgat lo- cality): a – ascapose form with prominent pulvinus (bulb without outer tunics), b – scapose form with small pulvinus, c – flower, d – gynoecium, e – seed micromorphology. Scale bar: a-d – 1 mm, e – 200 μm. RAT M., BOGDANOVIĆ S. 58 ACTA BOT. CROAT. 80 (1), 2021 out bulbils; outer tunics light brown to brown. Leaves nu- merous, glabrous, longer than inflorescence, up to 3.5 mm wide, canaliculate, with median white stripe on the adaxial side. Scape short, mostly hypogeal, 1 – 5 cm long. Inflores- cence up to 20 mm long, corymbose, with 1 – 8 (12) flowers. The overall length (scape + inflorescence) is ca 5 cm. The lower pedicels refracted, 10 – 25 mm long, with the pulvi- nus at the base. Bracts shorter than or equal to pedicels. Outer tepals 16 – 20 × 4 – 7 mm, with abaxial green stripe 4 – 6 mm wide. Inner tepals 15 – 21 × 5 – 8 mm, with ab- axial green stripe 3 – 6 mm wide. Filaments 7 – 11 mm long; anthers 2.5 – 5 mm long. Ovary elongated to rounded, 5 – 6 × 3.5 – 5.5 mm, with 6 prominent ribs; style 4 – 6 mm long, longer than or equal to ovary. Seeds globose, 1 – 2 mm in diameter, black, luminous, with reticulate testa. Phenology – Flowering and fruiting time from March to June. Leaf anatomy – Leaf has typical “umbellatum-type”, that is “U” shape on transverse cross section. Leaf blade is cana- liculate, on abaxial side with 3 – 6 ribs. Longitudinal white stripe visible on the adaxial side is a consequence of non- continuous palisade tissue in the central part. Visible on the cross section are 1-layered epidermis, 1-layered palisade tis- sue and mesophyll. Vascular bundles are arranged in two lines: larger bundles are in the centre of mesophyll, and smaller ones along palisade tissue of abaxial side. Other than vascular bundles, mesophyll contains large and small cavities, later usually with raffid crystals (Fig. 2). Karyology – For karyological studies, bulbs were available from localities Brgat, Donji Lapac and Nin. All investigated individuals were diploid, 2n=2x=18 (Fig. 3a). Combined chro- mosome formula is 2n=2x=6m + 12sm, and Stebbins karyo- type asymmetry degree is 2A. Two chromosome pairs are long, three of medium length and four pairs are small chro- mosomes. Karyotype is characterized with chromosomes that have more or less gradual transition in size (Fig. 3b). Two pairs are metacentric, while others are submetacentric, with total haploid chromosome length 59.01±0.89 µm (Tab. 2). Sat- ellited chromosomes were not detected. Fig. 2. Leaf “U” cross section of Ornithogalum sibthorpii (Croatia, material from Brgat locality). Scale bar: 50 μm. Tab. 2. Chromosome parameters for Ornithogalum sibthorpii in Croatia. Ten individuals are analyzed (2n=2x=18): four from Donji Brgat, three each from Nin and Donji Lapac. Number of analyzed metaphase plates is 40 in total (four per individua). Abbreviations: L – long arm length, S – short arm length, TAL – total absolute length, TRL – total relative length, m – metacentric, sm – submetacentric. Chromosome pairs L (µm) S (µm) TAL (µm) TRL (%) Type I 6.20±0.72 5.13±1.03 11.32±1.39 10.51+8.68=19.19 m II 5.96±0.48 3.12±0.65 9.08±0.84 10.10+5.29=15.38 m III 5.41±0.55 2.08±0.51 7.49±0.97 9.17+3.52=12.69 sm IV 4.52±0.99 2.18±0.50 6.71±1.30 7.66+3.70=11.36 sm V 4.17±0.71 2.01±0.54 6.18±0.98 7.07+3.41=10.47 sm VI 3.80±1.25 1.67±0.41 5.47±1.27 6.44+2.83=9.27 sm VII 3.04±0.51 1.87±0.58 4.92±0.99 5.15+3.18=8.33 sm VIII 2.82±0.58 1.54±0.29 4.36±0.62 4.78+2.61=7.39 sm IX 2.10±0.82 1.39±0.51 3.49±1.18 3.55+2.36=5.91 sm Fig. 3. Mitotic metaphase chromosome plate of Ornithogalum sibthorpii (Croatia, from Brgat locality): a – metaphase plate; b – karyo- gram (2n=2x=18). Scale bar: 10 μm. ORNITHOGALUM SIBTHORPII IN CROATIA ACTA BOT. CROAT. 80 (1), 2021 59 Distribution and habitat – First herbarium specimens that confirmed presence of O. sibthorpii in Croatia were found in the collection of Ivo Horvat (ZAHO), and date back to the first half of the 20th century. Since then this spe- cies was omitted in collections, most probably due to the short vegetation period and early spring flowering time. However, herbarium revision of collections in GZU, W and WU revealed that the species was recorded earlier, but sparse data led to its neglect in Croatia. Recent field trips expand- ed the known distribution range of O. sibthorpii in Croatia. It is spread from the southeast of the eastern Adriatic cost to the central Dalmatian region in the west, and in the north the central Dinaric region, which is the only continental lo- cation in Croatia. According to all the data gathered, a dis- tribution map was created (Fig. 4). O. sibthorpii inhabits dry, clear, open habitats, dry hillsides as well as anthropogenic areas and bare surfaces. The estimated altitude in Croatia ranges from sea level up to 1000 m. In other regions of the Balkan Peninsula habitats and altitude are similar (Raden- kova 1964, Rat et al. 2014, Rat and Barina 2017, Rat 2019). Examined specimens (specimina visa) – Croatia: Lika, Krbava – Donji Lapac, Bare, u nižem vlažnijem dijelu polja, 07.06.1958, I. Horvat s.n. (ZAHO-41129, ZAHO-41130); Makarska (Dalmat.), 17.04.1931, M. Salzmann s.n. (GZU- 057466); Orebić (Dalmatien), 16.04.; 19.04.1930, M. Salz- mann s.n. (GZU 057466); Süddalmatien, Halbinsel Pelješac: zw. Orebić u. Sattel östl. des, Mte. Vipera, 16.04.1930, J. Egg- ler s.n. (GZU 103693); Süddalmatien, Halbinsel Peljesac: Kräuterfluren u. Macchie westl. von Orebic., 14.04.1930, J. Eggler s.n. (GZU 103694); Dalmatien, Gravosa [Dubrovnik], 10.04.1933, K. Ronniger s.n. [sub Ornithogalum exscapum Ten.] (W, Herbarium Karl Ronniger 5580); Dalmatia, in vi- neis pr. Spalato [Split], 04.1870, Pichler s.n. [sub Ornithoga­ lum umbellatum L. (Vis.)] (WU, Herbarium Kerner). Identification key for O. sibthorpii and morphologically similar species in Croatia 1. Bulb with numerous bulbils (>10) outside of tunics; bracts equal to or longer than pedicels ..... O. refractum – Bulb without bulbils; bracts equal to or shorter than pedicels .............................................................................. 2 2. Scape hypogeous, 20–50 mm long; inflorescence 1–8 (15)-flowered; lower pedicels refracted at anthesis, up to 30 mm long; pulvinus prominent; bracts subequal the pedicels ................................................... O. sibthorpii – Scape only partly hypogeous, 10–30 mm long; inflo- rescence 5–10-flowered; pedicels erect to divergent (basal pedicel), 35–42 mm long; pulvinus absent; bracts shorter than pedicels ....................... O. exscapum Discussion This species has been known since the pre-Linnean pe- riod. Buxbaum (1728) described it for the first time from the region around Istanbul (Constantinopolium) in Turkey and he also provided the first iconography of the species. It was overlooked by Linne, but later botanists who investigated Greece and the eastern Mediterranean, Sibthorp and Smith, described Ornithogalum nanum as new species. Ornitho­ galum nanum Smith in Sibthorp and Smith (1809) was Fig. 4. Distribution map of Ornithogalum sibthorpii in Croatia. RAT M., BOGDANOVIĆ S. 60 ACTA BOT. CROAT. 80 (1), 2021 described as: „O. nanum, corymbo simplici paucifloro gla- bro scapo longiore, bracteis ventricosis scariosis, foliis lin- earibus numerosis“. The locality is cited as “In Arcadia, et prope Abydum, Martio florens”, with reference to Buxbaum (1728). Abydum is ancient name for the region close to to- day’s Cannakalle in Turkey, and Arcadia is an ancient re- gion in Peloponnese, but also in Antalya (Turkey) (Stearn 1967). Because of this, the precise locus classicus is not known. A detailed description of the species was published later (Sibthorp and Smith 1823). Herbarium specimen marked as type material is deposited in OXF herbarium, under the file number Sib-0793. Since O. nanum is a later homonym of O. nanum (Burm. f.) Thunb., replacement name O. sibthorpii was published by Greuter (Greuter and Rechinger 1967), in honour of John Sibthorp (1758–1796) who collected plant material (Stearn 1984). Although in most of the relevant databases (e.g. The- PlantList, Euro+Med Checklist) O. sibthorpii is synonymised with O. sigmoideum Freyn et Sint. we retain viewpoint of Landström (1989), later supported by the results of Speta (1990), that O. sibthorpii is a good species which should not be underestimated without systematic investigations. The results of field trips organized in 2015 and 2016 con- firmed our assumptions that O. sibthorpii is distributed in Croatia, and these data were presented by Rat and Bogdanović (2016) at the 5th Croatian Botanical Sympo- sium. Based on this report and deposited herbarium speci- mens in ZAGR herbarium (ZAGR-55520, 55521), Nikolić (2019a, b) included it in the national list of vascular flora. Historical data about distribution in Italy (Parlatore 1857) however have to be revised, since this record was included in distribution data for the species O. mutabile De Not., which is in meantime synonymised with O. exscapum by Peruzzi and Passalacqua (2002) and Garbari et al. (2003). Landström (1989) summarized differential morphological characters of O. sibthorpii and O. exscapum, describing O. sibthorpii with shorter pedicels, larger flowers, longer styles and anthers. An analysis of the material from Croatia shows that the additional differential qualitative morphological characters for these two species are: pedicel shape (in O. sib­ thorpii refracted at anthesis, in O. exscapum ascending to deflexed), visible pulvinus in O. sibthorpii, and absence of pulvinus in O. exscapum. Another informative parameter is bract/pedicel length ratio; in O. sibthorpii bract and pedi- cel are almost of the same length, while in O. exscapum bract is about half the length of the pedicel. Comparative morpho-anatomical and cytotaxonomical studies of Ornithogalum species that belong to the group of small plants (overall length up to 10 cm) with hypogeal scape, including O. sibthorpii, have been done to clearly de- scribe morphologically similar species in the area of Turkey and the Balkan Peninsula (Zahariadi 1962, 1965, 1977, Spe- ta 1990, 2000). Both authors recognized three separated species: O. sintenisii Freyn, O. sigmoideum and O. sibthorpii. In addition, Speta (1990) described two new species, O. sag­ inatum Speta and O. plutullum Speta, first from Romania and Moldavia, and later from the Balkan Peninsula. Moreover, Landström (1989) recognized two ecotypes (montane and lowland) of O. sibthorpii and compare them to the intermediate type, localised for the region of İstanbul (Turkey). These ecotypes are confirmed in Croatia as well, since for plants collected at higher altitudes in the continen- tal region (Donji Lapac, Lika, Dinaric area, 900 m a.s.l.) the flowering time is in June. In Croatia, two distinct morpho- types of O. sibthorpii are observed: ascapose and scapose. The ascapose form (Fig. 1a) is characterized by a short scape, and the inflorescence is more or less sessile on the ground, with numerous flowers; pulvinus is strong and prominent. The scapose form (Fig. 1b) is differentiated with a scape up to 5 cm long, and an inflorescence with only a few (1 to 2) flowers (Fig. 1b). Comparing our results with those of Speta (1990) who investigated O. sibthorpii and re- lated species in the eastern part of the Balkan Peninsula, we can confirm that both morphotypes are mostly present in the same population. Going further, he indicated that it is not unusual for two or more species from this group, that are morphologically indivisible, to coexist in one locality. In the Balkan Peninsula, O. sibthorpii could be easily misidentified with O. refractum. The most informative dis- criminate characteristic is a bulb without bulbils in O. sib­ thorpii (Landström 1989, Rat et al. 2014). It is not unusual to find both species in close localities, when in addition to bulb parameters flower characteristics can contribute to species identification (Rat et al. 2014). Furthermore, accord- ing to Rat et al. (2017) seed testa micromorphology can be used as a taxonomically important character for the differ- entiation of O. sibthorpii from other Ornithogalum species. Globose seeds are typical only for O. sibthorpii (Fig. 1e) and O. refractum, while, former is differentiated as species with rather small seeds. Cytological review by Cullen and Ratter (1967), with doubtful discussion on plant material, reported three main cytotypes for O. sibthorpii ‒ O. sigmoideum group, in the region from Caucasus to Italy, with numerous aloploid se- ries reported from different sources (2n = 12, 16, 17, 18, 19, 20, 28). They did not undertake any morphological mea- surements, and it was impossible for them to compare mor- photypes and cytotypes or to discuss in detail obtained results. Nonetheless, they recognized only two species, O. sigmoideum and O. sintenisii, while for them O. sibthorpii was a synonym of O. sigmoideum. An opposite view was presented by Speta (2000), who included both morpho- - anatomical and cytological reports for 18 species that belong to this group. He recognized all species based on taxonomical characters, including the karyotypes de- scribed. It is clear from his results that O. sibthorpii is sepa- rated from all other species, with 2n = 14, 28 chromosome complements, while in O. sigmoideum they are 2n = 20, 24. Chromosome arrangement 2n = 18 was documented also by Speta (1990) in an unresolved discussion in short notes. In addition to these species, for O. sintenisii chromosome number 2n = 12 is recorded. Diverse chromosome numbers (2n = 14, 16, 18, 24, 28) for O. sibthorpii were recorded and documented from the Balkan Peninsula, by other authors ORNITHOGALUM SIBTHORPII IN CROATIA ACTA BOT. CROAT. 80 (1), 2021 61 as well. For Bulgaria, Markova (1972) reported three differ- ent chromosome numbers (2n = 14, 16, 28), while Lungeanu (1971) reported 2n = 18, 24 for Romania. Following evolutionary patterns in different Ornithoga­ lum groups, it can be recognized that in almost all subgen- era there are several groups or complexes that are charac- terized with “high morphological variability” and extensive chromosome numbers among samples. By now, for most of them, systematic studies have been undertaken and it has been confirmed that every complex (i.e. O. tenuifolium in Africa and O. umbellatum in Europe) includes “good spe- cies” and “transitional forms” (cytotypes and morphotypes) that exist in nature thanks to vegetative reproduction (Sted- je and Nordal 1984, 1987, van Raamsdonk 1985, van Raams- donk and Heringa 1987, Andrić et al. 2015). Stedje and Nor- dal (1984) concluded that rapid evolutionary processes in Ornithogalum, visible as cytotype differentiation, do not ex- press their changes in morphological characters, but do in- dicate the evolutionary progress in the taxon. 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