COLONIZATION OF BACTERIA AND DIATOMS IN A MARINE LAKE ACTA BOT. CROAT. 79 (2), 2020 1 On-line Suppl. Tab. 1. Schedule of sampling physico-chemical data, bacteria and diatoms in Lake Mrtvo More in 2016. T – temperature, S – salinity, NO3– – nitrate, NO2– – nitrite, NH4+ – ammonium, TIN – total inorganic nitrogen, PO43– – phosphate, SiO44– – silicate, Chl a – chlorophyll a concentrations, O2/O2′ – oxygen saturation. Se as on M on th Date Physico-chemical parameter Bacteria samples Diatom samples T S NO3– NO2– NH4+ TIN PO43– SiO44– Chl a O2/O2' Sp ri ng A pr il 19-Apr-2016 1 1 1 1 1 1 1 1 1 1 . . 26-Apr-2016 1 1 1 1 1 1 1 1 1 1 . . M ay 4-May-2016 1 1 1 1 1 1 1 1 1 1 . . 10-May-2016 1 1 1 1 1 1 1 1 1 1 1 1 20-May-2016 1 1 1 1 1 1 1 1 1 1 . 1 25-May-2016 1 1 1 1 1 1 1 1 1 1 . 1 31-May-2016 1 1 1 1 1 1 1 1 1 1 . 1 Su m m er Ju ne 7-Jun-2016 1 1 1 1 1 1 1 1 1 1 1 1 18-Jun-2016 1 1 1 1 1 1 1 1 1 1 1 1 24-Jun-2016 1 1 1 1 1 1 1 1 1 1 1 1 29-Jun-2016 1 1 1 1 1 1 1 1 1 1 1 1 Ju ly 7-Jul-2016 1 1 1 1 1 1 1 1 1 1 . 1 13-Jul-2016 1 1 1 1 1 1 1 1 1 1 1 1 20-Jul-2016 1 1 1 1 1 1 1 1 . 1 1 1 28-Jul-2016 1 1 1 1 1 1 1 1 1 1 1 1 A ug us t 11-Aug-2016 1 1 1 1 1 1 1 1 1 1 1 1 17-Aug-2016 1 1 1 1 1 1 1 1 1 1 . 1 24-Aug-2016 1 1 1 1 1 1 1 1 1 1 1 1 A ut um n Se pt em be r 2-Sep-2016 1 1 1 1 1 1 1 1 1 1 1 1 6-Sep-2016 1 1 1 1 1 1 1 1 1 1 1 1 14-Sep-2016 1 1 1 1 1 1 1 1 1 1 . 1 21-Sep-2016 1 1 1 1 1 1 1 1 1 1 . 1 27-Sep-2016 1 1 1 1 1 1 1 1 1 1 . . O ct ob er 3-Oct-2016 1 1 1 1 1 1 1 1 1 1 . 1 12-Oct-2016 1 1 1 1 1 1 1 1 1 1 . 1 Number of samples: 25 25 25 25 25 25 25 25 24 25 12 21 On-line Suppl. Tab. 2. Results of ANOSIM test performed on physico-chemical data. Physico-chemical parameters varied significantly (ANOSIM, P < 0.05) among: seasons (spring, summer, autumn), months (April-October), between the significantly different clusters of samples for analysis physico-chemical parameters collected before the 18th June (Group 1) and afterwards (Group 2, and Group 3 contain- ing only sample from 12th October), and between the significantly different clusters of diatom assemblages. Av.Abund. – average abundance. Season Month Environmental Simprof Groups 1 & 2 & 3 Av. Abund. Simprof Groups 1 & 2 & 3 Av. Abund. Simprof Subgroups 1A & 1B & 2A & 2B & 3 P 0.001 0.001 0.001 0.001 > 0.05 Global R 0.545 0.466 0.891 0.386 0.381 CAR A, HAFNER D, LJUBIMIR S, DUPČIĆ RADIĆ I, BOBANOVIĆ-ĆOLIĆ S, JASPRICA N 2 ACTA BOT. CROAT. 79 (2), 2020 On-line Suppl. Tab. 4. List of diatoms taxa and their percentage contribution to total diatom community composition (taxa with relative abundances ≥ 3.5%, RA, are only shown) on artificial substrat (glass) in Lake Mrtvo More in 2016. TRIX index was computed in order to identify the trophic level of the research area. Groups and sub-groups were established upon the CLUSTER analysis with similarity profiles (SIMPROF) performed to determine significant levels of similarity between diatom samples. Only relative abundances > 25% are framed with black rectangle. T R IX O lig ot ro ph ic M es ot ro ph ic Ex tr em e Eu tr op hi c Eu tr op hi c M es ot ro ph ic Eu tr op hi c Ex tr em e Eu tr op hi c Eu tr op hi c M es ot ro ph ic O lig ot ro ph ic Group 1 2 3 Subgroup 1A 1B 2A 2B Month May June July August September October Taxa / Date 10 -M ay 20 -M ay 25 -M ay 31 -M ay 07 -J un 18 -J un 24 -J un 29 -J un 07 -J ul 13 -J ul 20 -J ul 28 -J ul 11 -A ug 17 -A ug 24 -A ug 02 -S ep 06 -S ep 14 -S ep 21 -S ep 03 -O ct 12 -O ct Achnanthes kuwaitensis 11 .1 4 8. 75 Amphora sp. 10 .0 0 3. 50 Cocconeis costata 6. 75 3. 50 7. 25 6. 00 4. 73 8. 50 10 .0 0 6. 25 6. 75 4. 09 Cocconeis dirupta var. flexella 22 .5 0 14 .7 5 20 .7 5 19 .7 5 5. 50 29 .8 5 51 .0 0 37 .2 5 65 .0 0 28 .8 9 47 .0 0 27 .5 0 24 .0 0 15 .5 0 11 .8 8 23 .7 5 15 .5 0 10 .0 0 8. 25 6. 27 Cocconeis dirupta 4. 02 Cocconeis pseudomarginata 3. 75 7. 47 Cocconeis scutellum var. scutellum 53 .2 5 73 .0 0 73 .0 0 70 .7 5 89 .2 5 47 .2 6 39 .0 0 55 .5 0 7. 25 17 .0 9 14 .7 5 10 .0 0 4. 25 7. 00 6. 00 9. 75 4. 09 Cocconeis woodii 4. 50 On-line Suppl. Tab. 3. Results of ANOSIM test performed on diatom species (S) and growth form (GF) relative abundance data. Diatom assemblages differed significantly (ANOSIM, P < 0.05) among months, between the significantly different clusters of diatom samples col- lected up to the middle of July (Group 1) and afterwards (Group 2, and Group 3 containing only sample from 12th October), and between the significantly different sub-clusters of diatom samples. Season Month Simprof Groups 1 & 2 & 3 Simprof Subgroups 1A & 1B & 2A & 2B & 3 S GF S GF S GF S GF P > 0.05 > 0.05 0.001 0.001 0.001 0.001 0.001 0.001 Global R 0.268 0.274 0.650 0.591 0.903 0.668 0.968 0.774 COLONIZATION OF BACTERIA AND DIATOMS IN A MARINE LAKE ACTA BOT. CROAT. 79 (2), 2020 3 T R IX O lig ot ro ph ic M es ot ro ph ic Ex tr em e Eu tr op hi c Eu tr op hi c M es ot ro ph ic Eu tr op hi c Ex tr em e Eu tr op hi c Eu tr op hi c M es ot ro ph ic O lig ot ro ph ic Group 1 2 3 Subgroup 1A 1B 2A 2B Month May June July August September October Diploneis crabro 6. 50 Fragilaria sp.2 13 .7 5 6. 50 Halamphora coffeiformis 3. 50 3. 75 7. 93 13 .5 0 3. 75 Halamphora hyalina 9. 50 11 .2 5 14 .3 6 6. 50 Halamphora subangularis 3. 50 5. 72 Licmophora flabellata 13 .5 0 8. 29 3. 75 12 .2 6 Licmophora paradoxa 7. 50 3. 75 3. 50 16 .8 9 43 .6 7 Mastogloia cuneata 5. 47 Navicula directa 3. 75 Navicula flagellifera 4. 50 6. 00 Navicula salinicola 3. 50 5. 25 13 .2 5 6. 94 10 .0 0 13 .2 5 3. 81 Navicula sp.1 7. 67 Nitzschia compressa var. compressa 4 .0 2 Nitzschia frustulum 5. 03 On-line Suppl. Tab. 4. Continued CAR A, HAFNER D, LJUBIMIR S, DUPČIĆ RADIĆ I, BOBANOVIĆ-ĆOLIĆ S, JASPRICA N 4 ACTA BOT. CROAT. 79 (2), 2020 T R IX O lig ot ro ph ic M es ot ro ph ic Ex tr em e Eu tr op hi c Eu tr op hi c M es ot ro ph ic Eu tr op hi c Ex tr em e Eu tr op hi c Eu tr op hi c M es ot ro ph ic O lig ot ro ph ic Group 1 2 3 Subgroup 1A 1B 2A 2B Month May June July August September October Nitzschia laevis 5. 25 Nitzschia sp.2 3. 73 Opephora mutabilis 4. 00 4. 00 7. 67 6. 00 10 .0 0 6. 25 5. 50 22 .3 4 Pinnularia quadratarea var. cuneata 5 .0 0 Pinnularia sp. 6. 50 Placoneis flabellate 3. 50 Psammodictyon rudum 8. 29 6. 25 Rhabdonema adriaticum 5. 50 7. 00 Seminavis sp. 3. 75 Striatella unipunctata 5. 18 Trachyneis aspera 19 .0 0 Tryblionella coarctata 5. 50 4. 28 On-line Suppl. Tab. 4. Continued COLONIZATION OF BACTERIA AND DIATOMS IN A MARINE LAKE ACTA BOT. CROAT. 79 (2), 2020 5 On-line Suppl. Fig. 1. A – Lake Mrtvo More on 7th July 2016, red dot indicates the sampling site position, B – plate with microscopic slides submerged in Lake Mrtvo More at the depth of 1 m on 19th of April 2016, C-E – hauling up the plate with microscopic slides so one glass could be removed for diatom analyses (C, D – 7th June 2016, E – 14th September 2016). On-line Suppl. Fig. 2. The average monthly precipitation (mm) in Dubrovnik for the period 1961-2017 and during 2016 (data for Du- brovnik meteorological station, Croatian Meteorological and Hydrological Service). CAR A, HAFNER D, LJUBIMIR S, DUPČIĆ RADIĆ I, BOBANOVIĆ-ĆOLIĆ S, JASPRICA N 6 ACTA BOT. CROAT. 79 (2), 2020 On-line Suppl. Fig. 3. Cluster analysis (A) and non-metric multidimensional scaling (NMDS) ordination (B) based on the data of physico- chemical parameters (temperature, salinity, TIN, PO43–, SiO44–, chlorophyll a concentrations, oxygen saturation, NO3–, NO2–, NH4+) in 25 sampling dates (Lake Mrtvo More, the island of Lokrum, April-October 2016). Euclidean distance as a similarity measure was used. N = 25.