Acta Herpetologica 12(1): 65-77, 2017 ISSN 1827-9635 (print) © Firenze University Press ISSN 1827-9643 (online) www.fupress.com/ah DOI: 10.13128/Acta_Herpetol-18737 Skeletal variation within the darwinii group of Liolaemus (Iguania: Liolaemidae): new characters, identification of polymorphisms and new synapomorphies for subclades Linda Díaz-Fernández*, Andrés S. Quinteros, Fernando Lobo Instituto de Bio y Geociencias del NOA, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Salta, Av. 9 de Julio 14, 4405 Rosario de Lerma, Salta, Argentina *Corresponding author. E-mail: lindadiazfernandez@gmail.com Submitted on: 2016, 19th August; revised on: 2016, 27h October; accepted on: 2017, 12th March Editor: Aaron M. Bauer Abstract. Fifty-five skeletal characters (continuous and discrete) were analyzed for species of the L. darwinii group: L. albiceps, L. chacoensis, L. grosseorum, L. irregularis, L. koslowskyi, L. lavillai, L. ornatus, L. quilmes, plus L. inacayali (L. telsen group) and L. scapularis (L. wiegmannii group). We report polymorphic intraspecific variation that has not pre- viously been taken into account and we describe 21 new characters that provide original information across the group. We detected several morphological synapomorphies for the darwinii group and subclades. The enclosure of Meckel’s cartilage by a dentary outgrowth on lingual side of lower jaw (a synapomorphy of the subgenus Liolaemus sensu stricto and of the Phymaturus patagonicus group) also occurs within the L. darwinii group. The morphology of maxillary teeth with three conspicuous cusps may be a potential synapomorphy of the subgenus Eulaemus. The morphology of maxillary teeth may have adaptive value. Characters that were studied in other groups of lizards were informative for Liolaemus. Keywords. Cranial skeleton, postcranial skeleton, L. boulengeri group, evolution. INTRODUCTION Osteological characters are used for phylogenetic analyses and reconstructions and for investigating envi- ronmental adaptations because skeletons exhibit a very wide range of morphological variation at supraspecific levels. Detailed examples that illustrate their use in sys- tematic and phylogenetic studies within Iguania are found in Etheridge (1965, 1967), de Queiroz (1987), Estes et al. (1988), Etheridge and de Queiroz (1988), Lang (1989) and Frost and Etheridge (1989), among others. Although this information was primarily used in phy- logenetic reconstructions, it is also important for future works in studies of comparative biology, as it can docu- ment evolutionary changes of characters in the context of any hypothesized selective regime. Lizards of the Family Liolaemidae extend from the Andes through Bolivia, Peru and Chile to the coast of Tierra del Fuego in Argentina (Donoso-Barros, 1966; Cei, 1986, 1993; Lobo and Quinteros, 2005; Abdala, 2007). Currently, this family has 298 species (Uetz, 2016) and comprises three genera: Ctenoblepharys, Liolaemus, and Phymaturus (Etheridge, 1995; Frost et al., 2001; Schulte et al., 2003; Espinoza et al., 2004). Within the subgenus Eulaemus of Liolaemus, there is the species-rich L. bou- lengeri group (Etheridge, 1995; Abdala, 2007), which is characterized by having a group of enlarged scales at the back of the thigh, so it is also called the “patch group”. As a result of taxonomic and phylogenetic studies of the L. boulengeri group, numerous subgroups within it have been proposed (Avila et al., 2006; Abdala, 2007). One of these is the Liolaemus darwinii clade, which was 66 L. Díaz-Fernández, A.S. Quinteros, F. Lobo defined by Etheridge (1993) based on the presence of posterior teeth with straight-edged crowns and marked sexual dichromatism in which males exhibit a more colorful dorsal color pattern than females. This group was inferred to be monophyletic in different analyses, based on morphological and/or molecular characters (Abdala, 2007; Avila et al., 2006; Fontanella et al., 2012; Olave et al., 2014). Following Abdala’s total evidence hypothesis (2007), the L. darwinii group consists of two clades (L. grosseorum and L. ornatus clades) and of five species basal to these ones (L. abaucan, L. espinozai, L. koslowskyi, L. quilmes and L. uspallatensis). The L. ornatus clade comprises 6 species (L. albiceps, L. crepuscularis, L. calchaqui, L. irregularis, L. lavillai and L. ornatus), which have a wide distribution in the Puna, Montes de Sierras y Bolsones and the northern part of the ecoregion of Monte de Llanuras y Mesetas of Argentina (Burkart et al., 1999). Lizards in this group are distinguished by being viviparous and having a large number of precloacal pores in females. Information about osteological features of liolaemid lizards appears in detailed descriptions of the head skel- eton of Liolaemus lutzae, L. occipitalis, and L. signifer (Beurman and Vieira, 1980; Simoes-Lopes and Krause, 1988). In addition, studies of the appendicular skeleton of L. occipitalis (Keller and Krause, 1986) and the skel- eton of L. lutzae and L. multiformis simonsii (Beurman and Vieira, 1980) have been published. Osteological character states for Liolaemus and Phymaturus in the context of iguanian phylogenetic analysis at the generic level were recorded by Etheridge and de Queiroz (1988). Lobo and Abdala (2001; 2002) described the variation found in the skeleton of 24 species. They demonstrated the phylogenetic information contained in those charac- ters, recovering main clades and subclades of Liolaemus formally recognized in the literature. Additional skeletal characters were reported recently by Núñez et al. (2003) in the description of two new taxa (L. manueli and L. torresi). Da Silva and Verrastro (2007) described the axi- al skeleton of L. arambarensis and González-Marín and Hernando (2013) described the postcranial osteology of L. azarai. In the present contribution we report new characters and polymorphisms and we provide additional informa- tive characters for the Liolaemus darwinii group and sub- clades therein. We report the evolution of some charac- ters of special interest such as the enclosure of Meckel’s cartilage (a character traditionally studied in iguanian lizards), the morphology of maxillary teeth, the cartilagi- nous extremity of cervical rib IV, and the bladelike pro- cess on the posterior distal tibia mentioned by Etheridge (1995) and Lobo and Abdala (2001). MATERIALS AND METHODS A total of 30 adult specimens of the Liolaemus dar- winii group were studied (Appendix A). The species included were L. albiceps, L. chacoensis, L. grosseorum, L. irregularis, L. koslowskyi, L. lavillai, L. ornatus, and L. quilmes. We also included skeletons of L. scapularis (rep- resenting the L. wiegmannii group) and L. inacayali (L. telsen group) for outgroup comparisons, and examined characters described in Lobo and Abdala (2001, 2002) for 24 taxa belonging to different groups of Liolaemus. We studied specimens deposited in biological collections and collected some additional specimens of L. ornatus, L. scapularis, and L. quilmes, which were sacrificed by injec- tion with 10% sodium pentothal. They were fixed in 10% formalin and finally preserved in 70% ethanol. They are deposited in the herpetological collection of the Museo de Ciencias Naturales of the Universidad Nacional de Sal- ta (MCN), Instituto de Bio y Geociencias del NOA, and the Fundación Miguel Lillo (FML). The skeletons were prepared following the technique of differential stain- ing of bone and cartilage (Wassersug, 1976). This allows the observation of cartilaginous structures that are not visible in the dry skeletons. Measurements were taken using digital calipers, 0.01 mm precision, under a stereo- scopic microscope. In the case of smaller structures, the measurements were taken using a micrometer eyepiece. Nomenclature of bones, processes and foramina follow de Queiroz (1982), Keller and Krause (1986), Frost (1992), Etheridge (2000), Lobo and Abdala (2001), Lobo (2001, 2005) and Torres-Carvajal, (2004). In total, 55 characters, 19 continuous and 36 discrete, of the cranial and postcra- nial skeleton were examined. The discrete characters were coded as non-polymorphic binary, polymorphic binary, non-polymorphic multistate, and polymorphic multistate. We add our data matrix to that of Abdala’s (2007) Total Evidence analysis and performed a new phylo- genetic analysis. Character evolution mapping and the optimization of new characters were performed using TNT (Goloboff et al., 2003) over the resulting tree (same topology as recovered by Abdala 2007; Fig. 1). We follow Abdala (2007) because it includes the taxa studied here (instead of Fontanella et al., 2012 or Olave et al., 2014; whose studies lacked many of the species of interest). Data on diet were taken from the literature (Aun and Martori, 1998; Espinoza et al., 2004; Semhan et al., 2013). RESULTS All morphological variation observed in the skeletons of Liolaemus is summarized in the following list of char- 67Skeletal variation in the Liolaemus darwinii group acters, indicating the state of the character in each case. Variation of discrete and continuous characters is pre- sented in Tables 1 and 2, respectively. Updated list of osteological characters (modified from Lobo and Abdala, 2002) 1. Number of scleral ossicles: (0) 15; (1) 14; (2) 13. Polymorphic multistate. 2. Bones forming parietal foramen: (0) formed mainly by frontal bone; (1) mainly by parietal bone; (2) both bones participate approximately equally. Polymorphic multistate. 3. Shape of parietal foramen: (0) with regular edges; (1) with irregular edges. Polymorphic binary. 4. Ceratohyal process: (0) gradually widened; (1) abruptly widened; (2) hook-shaped. Polymorphic multistate. 5. Distal ending of ceratobranchial II: (0) narrow; (1) widened. Polymorphic binary. 6. Anterior process of arytenoid: (0) reaches the level of the anterior process of the cricoid; (1) does not reach that level. Polymorphic binary. 7. Number of tracheal rings. Continuous (Table 2). 8. Number of incomplete tracheal rings / total number of rings. Continuous (Table 2). 9. Number of pterygoid teeth. Continuous (Table 2). 10. Number of maxillary teeth. Continuous (Table 2). 11. Number of modified anterior maxillary teeth (hetero- donty): in most species the anterior-most teeth of the maxilla are conical, elongated, and exhibit only one cusp. Continuous (Table 2). 12. Maxillary tooth morphology I (Fig. 2): (0) crowns with their anterior and posterior margins divergent, expanded crowns; (1) crowns with anterior and pos- terior margins straight. Non-polymorphic binary. 13. Maxillary tooth morphology II: (0) crowns without differentiated cusps, (1) three conspicuous cusps (all species studied here). Species of the L. nigromacula- tus group (subgenus Liolaemus sensu stricto) were reported as having broad maxillary teeth without sec- ondary cusps (Lobo, 2001). Non-polymorphic binary. 14. Meckel’s groove (Fig. 3): (0) open; (1) fused, Meckel’s cartilage is hidden by an extensive outgrowth of the dentary bone. This last character state was described as an apomorphy of the L. chiliensis group (sub- Table 1. Discrete osteological characters and their variation within the Leiolaemus darwinii group. Intraspecific variation indicated by in brackets surrounding the relevant carácter states. Polymorphism in characters 1, 4, and 20, have not previously been reported. 1 2 3 4 5 6 12 13 14 15 16 18 19 20 21 22 23 33 L. albiceps [02] 0 [01] 1 0 [01] 0 1 0 1 [01] 1 0 0 1 1 [12] 0 L. chacoensis [12] [02] [01] 1 0 0 1 1 1 [01] [01] 1 0 0 [01] 0 [12] 0 L. grosseorum 2 0 0 1 0 0 1 1 1 1 [01] 1 0 0 [01] 1 [12] 0 L. inacayali [12] [01] 0 2 0 0 0 1 0 1 0 1 0 0 1 1 2 0 L. irregularis 1 0 [01] 1 0 [01] 0 1 0 1 [01] 1 0 0 [01] 1 2 0 L. koslowskyi [12] [012] [01] 1 [01] 1 0 1 1 1 [01] 1 [01] 0 [01] 1 [12] 0 L. lavillai 1 1 1 1 0 0 1 1 1 1 1 1 [01] 0 1 1 1 0 L. ornatus 1 [12] 1 1 0 0 0 1 0 1 1 1 0 0 0 1 [123] 0 L. quilmes 1 [02] 1 [01] 0 [01] 1 1 1 1 [01] 1 0 [01] [01] 1 1 0 L. scapularis 2 [02] 0 2 0 1 1 1 0 1 0 1 [01] 1 1 1 [12] 0 34 35 36 37 38 39 41 45 46 47 48 49 50 51 52 53 54 55 L. albiceps 0 1 0 [01] 0 1 [012] 0 [01] 1 0 0 0 0 [01] 0 1 0 L. chacoensis 0 0 0 [01] [01] 0 2 [01] [01] 1 1 [01] 1 0 1 1 1 [01] L. grosseorum 0 1 1 1 0 0 2 1 1 1 0 0 0 0 1 1 0 [01] L. inacayali 0 1 0 1 1 0 2 0 1 0 0 0 0 0 1 1 0 0 L. irregularis 0 1 0 0 1 1 2 1 [01] 1 0 0 0 0 [12] [01] 0 0 L. koslowskyi 0 1 0 1 1 0 0 [01] [01] 1 0 0 0 0 [01] 1 1 1 L. lavillai 0 0 0 0 1 0 2 1 [01] 1 0 0 0 0 1 0 1 0 L. ornatus 0 1 1 0 0 0 0 1 1 1 0 1 0 [01] [01] [01] 1 0 L. quilmes 0 1 1 0 0 0 [02] 1 [01] 1 1 1 [01] [01] [01] [01] 1 0 L. scapularis 0 1 1 1 0 0 2 1 [01] 1 0 0 0 0 1 [01] 0 0 68 L. Díaz-Fernández, A.S. Quinteros, F. Lobo genus Liolaemus) and it is the condition exhibited by the Phymaturus patagonicus group according to Etheridge (1995), Lobo et al. 2012 (its Fig. 7D). Non- polymorphic binary. 15. Cervical rib III: this rib, when present, is very small and remains cartilaginous. (0) present; (1) absent. Polymorphic binary. 16. Cartilaginous extremity of cervical rib IV (Fig. 4A-B): (0) bifurcated; (1) not bifurcated. Polymorphic binary. 17. Number of postxiphisternal elongated ribs: According to Etheridge (1995), Liolaemus species exhibit the most common pattern of postxiphisternal (“inscriptional ribs posterior to xiphisternals”), with their free endings bearing an elongated cartilage. Continuous (Table 2). 18. Posterior process of the sternum: (0) present; (1) absent (all species studied here). State (0) reported only for the L. pictus group by Lobo and Abdala (2001). Non-polymorphic binary. Table 2. Continuous osteological characters (see text for character descriptions). Above: min-max. below: mean + standard deviation. Chars Liolaemus albiceps (N=4) Liolaemus chacoensis (N=4) Liolaemus grosseorum (N=2) Liolaemus inacayali (N=1) Liolaemus irregularis (N=4) Liolaemus koslowskyi (N=3) Liolaemus lavillai (N=2) Liolaemus ornatus (N=4) Liolaemus quilmes (N=5) Liolaemus scapularis (N=2) 7 53-67 (61; 6) 52-52 (52; 0) 55-67 (61; 9) 52 56-75 (62; 9) 52-63 (59; 6.3) 49-60 (55; 7.8) 41-65 (55; 10.6) 47-57 (52; 5) 54-57 (56; 2.1) 8 0.2-0.3 (0.3; 0.04) 0.19-0.4 (0.3; 0.1) 0.23-0.25 (0.2; 0.01) 0.3 0.25-0.32 (0.3; 0.03) 0.16-0.27 (0.2; 0.05) 0.15-0.31 (0.2; 0.1) 0.2-0.39 (0.3; 0.08) 0.25-0.4 (0.3; 0.07) 0.17-0.26 (0.2; 0.06) 9 5-8 (7; 1.4) 0-4 (2.3; 1.7) 0-3 (1.5; 2.1) 7 5-6 (5.8; 0.7) 3-5 (4.3; 1.2) 4-4 (4; 0) 2-6 (3; 2) 1-3 (2.6; 0.9) 2-4 (3; 1.4) 10 16-18 (17; 1) 15-20 (17.6; 2) 16-18 (14; 1.4) 14 18-18 (18; 0) 16-16 (16; 0) 14-14 (14; 0) 16-16 (16; 0) 15-18 (17; 1.1) 12-14 (13; 1.4) 11 0-0 (0; 0) 0-0 (0; 0) 0-0 (0; 0) 0 0-0 (0; 0) 0-0 (0; 0) 0-0 (0; 0) 0-0 (0; 0) 1-1 (1; 1) 2-3 (2.5; 0.7) 17 3-7 (4; 2) 2-4 (3; 1.4) 2-4 (3; 1.4) 5 3-4 (3.5; 0.6) 2-3 (2.7; 0.6) 3-4 (3.5; 0.7) 4.58-5.5 (5; 0.4) 2-2 (2; 0) 3-4 (3.5; 0.7) 24 2.5-4 (3.3; 0.8) 0.3-0.4 (0.4; 0.04) 0.4 0.3 0.34-0.39 (0.38; 0.02) 0.33-0.37 (0.36; 0.02) 0.38-0.4 (0.39; 0.01) 0.36-0.39 (0.37; 0.01) 0.36-0.41 (0.39; 0.02) 0.3-0.4 (0.4; 0.04) 25 0.7-1 (0.8; 0.1) 0.6-0.8 (0.7; 0.08) 0.7-0.8 (0.7; 0.04) 0.7 0.7-0.9 (0.8; 0.06) 0.7-0.9 (0.8; 0.08) 0.8-0.81 (0.78; 0.04) 0.75-0.77 (0.76; 0.01) 0.74-0.85 (0.78; 0.05) 0.72 26 0.2-0.24 (0.21; 0.03) 0.18-0.23 (0.21; 0.02) 0.18-0.21 (0.2; 0.02) ? 0.24-0.3 (0.27; 0.03) 0.22-0.25 (0.24; 0.02) 0.24-0.27 (0.26; 0.02) 0.28-0.3 (0.29; 0.01) 0.19-0.28 (0.24; 0.04) 0.19-0.22 (0.21; 0.02) 27 1.8-3 (2.2; 0.5) 1.5-2.2 (1.8; 0.3) 2.8-3.4 (3.1; 0.5) 2.2 2-2.6 (2.3; 0.3) 1.7-3.5 (2.6; 0.9) 2.5-2.6 (2.6; 0.05) 1.9-3.8 (2.7; 0.9) 1.9-3.1 (2.3; 0.5) 1.7-2.4 (2.1; 0.5) 28 0.03-0.07 (0.05; 0.02) 0.01-0.09 (0.04; 0.04) 0.02-0.02 (0.02; 0) 0.05-0.05 (0.05; 0) 0.03-0.06 (0.05; 0.01) 0.02-0.09 (0.06; 0.04) 0.04-0.06 (0.05; 0.01) 0.04-0.08 (0.05; 0.02) 0.04-0.05 (0.05; 0.01) 0.02-0.06 (0.04; 0.02) 29 0.06-0.19 (0.13; 0.06) 0.1-0.26 (0.19; 0.07) 0.07-0.12 (0.1; 0.04) 0.19-0.19 (0.19; 0) 0.05-0.17 (0.11; 0.07) 0.06-0.19 (0.12; 0.07) 0.07-0.1 (0.09; 0.02) 0.03-0.17 (0.11; 0.06) 0.1-0.18 (0.14; 0.06) 0.05-0.2 (0.13; 0.06) 30 0.23-0.34 (0.29; 0.06) 0.26-0.33 (0.29; 0.03) 0.34-0.34 (0.34; 0) 0.27-0.27 (0.27; 0) 0.31-0.38 (0.34; 0.03) 0.28-0.34 (0.31; 0.03) 0.33-0.33 (0.33-0) 0.24-0.34 (0.28; 0.05) 0.35-0.35 (0.35; 0) 0.31-0.41 (0.35; 0.04) 31 0.04-0.11 (0.08; 0.04) 0.06-0.11 (0.09; 0.03) 0.1-0.1 (0.1; 0) 0.07-0.07 (0.07; 0) 0.05-0.11 (0.08; 0.03) 0.07-0.09 (0.08; 0.01) 0.06-0.07 (0.07; 0.01) 0.08-0.1 (0.09; 0.01) 0.06-0.06 (0.06; 0) 0.06-0.12 (0.08; 0.02) 32 0.76-0.93 (0.84; 0.09) 0.61-1.01 (0.82; 0.17) 0.76-0.79 (0.78; 0.02) 1-1 (1; 0) 0.72-1.04 (0.88; 0.14) 0.54-0.81 (0.66; 0.14) 0.83-0.89 (0.84; 0.04) 0.79-0.94 (0.86; 0.07) 0.84-0.88 (0.86; 0.03) 0.81-0.94 (0.88; 0.06) 40 8-7 (7.25;0.5) 6-3 (4.7;1.3) 5-3 (4;1.4) 6-6 (6; 0) 6-8 (6.7; 0.9) 5-12 (9;3.6) 5-8 (6.5; 2.1) 7-3 (4.7;1.7) 5-5 (5;0) 5-11 (6.6;2.5) 42 -4 (5.7;1.5) 4-4 (4;0) 5-5 (5;0) 4-4 (4;0) 4-6 (4.5; 1) 6-3 (4.67;1.63) 4-5 (4.5; 0.71) 8-7 (7.25;0.5) 7-7 (7;0) 5-4 (4.8;0.5) 43 6-6 (6; 0) 5-6 (5.7; 0.5) 6-6 (6; 0) 6-6 (6; 0) 6-6 (6; 0) 4-6 (4.7; 1.1) 6-6 (6; 0) 5-6 (5.25; 0.5) 5-6 (5.75; 0.5) 3-6 (15.4; 1.3) 44 20-22 (21; 0.8) 20-22 (20.5; 1) 18-20 (19; 1.4) 17-17 (17; 0) 22-24 (23; 1.1) 20-20 (20; 0) 18-18 (18; 0) 20-21 (20.75; 0.5) 18-20 (19; 1.4) 18-21 (19.8; 1.6) 69Skeletal variation in the Liolaemus darwinii group 19. Clavicles: (0) without fenestra; (1) with fenestra. Polymorphic binary. 20. Sternal fenestra (Fig. 4C-D) located in the posterior half of the sternum over the posterior half of inter- clavicle: (0) single; (1) divided, as described by Ether- idge (2000) for species of the L. wiegmannii group. Polymorphic binary. 21. The posterior end of hipoischium: (0) expanded; (1) unexpanded. Polymorphic binary. 22. Bladelike process on posterior distal tibia: (0) absent; (1) present. The presence of the bladelike process on the posterior distal tibia was proposed as a syna- pomorphy of the L. montanus group by Etheridge (1995) and as a synapomorphy of the subgenus Eulaemus (including the L. anomalus group accord- ing to Lobo et al., 2010). Non-polymorphic binary. 23. Caudal vertebrae without “chevron”: (0) caudal ver- tebra I; (1) caudal vertebrae I and II; (2) caudal ver- tebrae I-III; (3) caudal vertebrae I-IV. Polymorphic multistate. 24. Skull height / skull length. Continuous (Table 2). 25. Skull width / skull length. Continuous (Table 2). 26. Lateral rami of interclavicle / skull length. Continu- ous (Table 2). 27. Diameter of major coracoid fenestra / diameter of major scapular fenestra. Continuous (Table 2). 28. Preischial length / skull length. Continuous (Table 2). 29. Xiphisternal rod length / skull length. Continuous (Table 2). 30. Clavicle length / skull length. Continuous (Table 2). 31. Maximum clavicle width / skull length. Continuous (Table 2). 32. Sternal width / sternal length. Continuous (Table 2). 33. Orientation of the pubis: (0) facing forward, forming an acute angle with vertebral column; (1) perpendic- ular to the vertebral column. State (0) only reported for L. pseudoanomalus (Lobo and Abdala, 2001). Non-polymorphic binary. 34. Membranes over coracoid fenestrae: (0) without ossification; (1) with ossification. Non- polymorphic binary. Fig. 1. Phylogenetic relationships recovered for the Liolaemus dar- winii group. Fig. 2. Evolution of morphology of maxillary crowns and diet within the Liolaemus darwinii group. (A) Character states optimized on the topology recovered, (B) maxillary teeth with straight crowns, (C) maxillary teeth with expanded crowns. Dietary data for L. inacayali and L. lavillai were not found in the literature. Scale = 1 mm. Fig. 3. Evolution of Meckel’s groove within the Liolaemus darwinii group. (A) Character states optimized on the recovered topology, (B) Meckel’s groove of lower jaw closed, (C) open Meckel´s groove (C). Scale = 2 mm. 70 L. Díaz-Fernández, A.S. Quinteros, F. Lobo New characters found in the present contribution 35. Temporal fenestra (Fig. 4E-F): (0) open (without contact between postorbital and squamosal); (1) closed (contact between postorbital and squamosal). Non-polymorphic binary. 36. Posterior edge of parietal (Fig. 5A-B): (0) convex; (1) forming a straight margin. Non- polymorphic binary. 37. Posfrontal shape (Fig. 5C-D): (0) triangular; (1) elon- gated, not triangular. Polymorphic binary. 38. Premaxillary shape (Fig. 5E-F): (0) nasal spine nar- row and pars dentalis wide; (1) nasal spine wide and pars dentalis narrow (modified from Frost, 1992). Polymorphic binary. 39. Otic ramus of squamosal (Fig. 6A-B: (0) otic ramus located over the superior fossa of quadrate; (1) otic ramus inserted in the superior fossa of quadrate. Non-polymorphic binary. 40. Number of labial foramina (lateral view of maxilla). Continuous (Table 2). 41. Disposition of labial foramina (maxilla): (0) L-shaped; (1) forming two parallel rows; (2) forming a unique series in a single line. Polymorphic multi- state. 42. Number of mental foramina of dentary (lateral view). Continuous (Table 2). Fig. 4. Skeletal characters exhibiting variation within the Liola- emus darwinii group. (A-B) Cartilaginous extremity of cervical rib IV: bifurcate (A) in L. scapularis (MCN2431) and non-bifurcate (B) in L. ornatus (MCN 3545). Scale = 2 mm. (C-D) Sternal fenestra: single (C) in L. ornatus (MCN 3546) and divided (D) in L. quilmes (MCN 3524). Scale = 2 mm. (E-F) Temporal fenestra: open, with- out contact between postorbital and squamosal (E) in L. ornatus (MCN 3548) and closed, with contact between postorbital and squamosal (F) in L. koslowskyi (MCN 574). Scale = 1mm. Fig. 5. Skeletal characters exhibiting variation within the Liola- emus darwinii group. (A-B) Posterior edge of parietal: convex (A) in L. irregularis (MCN 2443) and forming a straight margin (B) in L. quilmes (MCN 3527). Scale = 1mm. (C-D) Posfrontal shape triangular (C) in L. quilmes (MCN 3527) and elongated (D) in L. inacayali (MCN 500). Scale = 1mm. (E-F) Premaxillary shape: nasal spine narrow and pars dentalis wide (E) in L. albiceps (MCN 402) and nasal spine wide and pars dentalis narrow (F) in L. koslowskyi (MCN 573). Upper arrow indicates the width of the nasal spine and bottom arrows indicate the width of the area of premaxillary teeth. Scale = 2 mm. 71Skeletal variation in the Liolaemus darwinii group 43. Number of premaxillary teeth. Continuous (Table 2). 44. Numbers of dentary teeth. Continuous (Table 2). 45. Lower jaw dentition (Fig. 6C-D): (0) homodont; (1) heterodont. Polymorphic binary. 46. First chevron shape (Fig. 6E-F): Chevron bones appear on anterior caudal vertebrae (Hoffstetter and Gasc, 1969). In Liolaemus the first chevron can appear on caudal vertebra III or IV. (0) incomplete; (1) complete. Polymorphic binary. 47. Length of metatarsus IV with respect to toe V (Fig. 6 G-H): (0) reaches phalanx II; (1) reaches phalanx III (modified from Arias, 2012). Non-polymorphic binary. 48. Length of IV metacarpal with respect to finger V: (0) reaches phalanx I; (1) reaches phalanx II. Non-poly- morphic binary. 49. Hipoischial fenestra: (0) absent; (1) present. Poly- morphic binary. 50. Ischial fenestra (located close to the posterior margin of the ischium): (0) absent; (1) present. Polymorphic binary. 51. Number of sternal ribs: (0) three; (1) four. Polymor- phic binary. 52. Number of branches of the xiphisternal rib: (0) three; (1) two; (2) none. Polymorphic binary. Fig. 6. Skeletal characters exhibiting variation within the Liolaemus darwinii group. (A-B) Otic ramus of squamosal located outside to the superior fossa of quadrate (A) in L. irregularis (MCN 2436) and otic ramus inserted in the superior fossa of quadrate (B) in L. gros- seorum (MCN 508). Arrow indicates the insertion of the squamosal in the superior fossa of the quadrate. Scale = 2mm. (C-D) Lower jaw dentition homodont (C) in L. inacayali (MCN 500) and hetero- dont (D) in L. quilmes (MCN 3525). Scale = 1mm. (E-F) First chev- ron condition: incomplete (E) in L. quilmes (MCN 3524) and com- plete (F) in L. albiceps (MCN 402). Scale = 3mm. (G-H). Length of metatarsal IV with respect to toe V: reaches phalanx III (G) in L. irregularis (MCN 2431) and reaches phalanx II (H) in L. inacayali (MCN 500). Scale = 1 mm. Fig. 7. Skeletal characters exhibiting variation within the Liolaemus darwinii group. (A-B) Presence of open intercalated tracheal rings: present (A) in L. lavillai (MCN 4351) and absent (B) in L. gros- seorum (MCN 509). Scale = 1 mm. (C-D) Lateral processes of the cricoid: not pronounced (C) in L. irregularis (MCN 2443) and pro- nounced (D) in L. albiceps (MCN 457). Scale = 1mm. (E-F) Sternal fenestra shape: symmetrical not widened (E) in L. albiceps (MCN 457) and wider in the posterior half (F) in L. chacoensis (MCN 599). Scale = 1mm. 72 L. Díaz-Fernández, A.S. Quinteros, F. Lobo 53. Open intercalated tracheal rings (Fig. 7A-B): (0) pre- sent; (1) absent. Polymorphic binary. 54. Lateral processes of the cricoid (Fig. 7C-D): (0) not pronounced; (1) pronounced. Non-polymorphic binary. 55. Shape of sternal fenestra (Fig. 7E-F): (0) symmetrical, not widened; (1) wider in the posterior half. Poly- morphic binary. DISCUSSION In this contribution, twenty one new characters for the genus Liolaemus were studied (characters from 35 to 55). We also report additional states for characters pre- viously described (Table 1). First, the number of scle- ral ossicles was previously reported by Lobo and Abdala (2001) as binary polymorphic (13 or 14 ossicles), whereas here we found a new state for L. albiceps (15 ossicles), we consequently coded the character as polymorphic multistate. Second, the ceratohyal process was coded in Lobo and Abdala (2001) as non-polymorphic multi- state (gradually widened, abruptly widened, and hook- shaped), whereas here we found in L. quilmes a polymor- phism (gradually widened and abruptly widened). Third, according to Lobo and Abdala (2001), the sternal fenestra can be divided or single, without polymorphism. In this study we report a polymorphism in L. quilmes, which may have a divided or undivided sternal fenestra. The specimens of L. cf. quilmes included in Lobo and Abdala (2001) correspond to L. crepuscularis (Abdala and Diaz Gómez, 2006), a species distinct from that included in this contribution as L. quilmes. Maxillary teeth with three conspicuous cusps were found in all specimens studied. This is consistent with Lobo and Abdala (2001), who found this character state in all specimens of the subgenus Eulaemus, whereas the species of the L. nigromaculatus group (belonging to the subgenus Liolaemus sensu stricto) show the maxillary teeth without differentiated cusps. This evidence allows us to consider tricuspid maxillary teeth as a potential synapomorphy of Eulaemus. The number of tracheal rings for species of the Liola- emus boulengeri group reported by Lobo and Abdala (2001) ranges from 48 to 67. We extend this range to 41-75. A potential synapomorphy for the Liolaemus darwi- nii group (Fig. 8) involves the morphology of the termi- nal cartilage of cervical rib IV, which is narrow and not bifurcated in Liolaemus albiceps, L. chacoensis, L. grosse- orum, L. irregularis, L. koslowskyi, L. lavillai, L. ornatus, and L. quilmes (though polymorphic in the latter). Lobo and Abdala (2001) found this character to be polymor- phic in L. koslowskyi. Etheridge (1993) defined the Liolaemus darwi- nii complex based on the “possession of maxillary teeth crowns with straight edges” as an exclusive character of this group. Liolaemus scapularis (a member of the L. wiegmannii group), also shows tooth crowns with straight edges, so this character state is not exclusive to the L. darwinii complex, as Etheridge (1993) proposed. Moreo- ver, our results indicate variation within this group (Fig. 2A) and according to recognized relationships (Abdala, 2007), this character has changed in the terminal sub- clade of the L. darwinii group (the L. ornatus group). Therefore, it can be considered a synapomorphy of the L. ornatus group (Fig. 2A-C). Optimizing the character states of maxillary tooth crowns and the diet in the tree recovered, we found that an insectivorous diet and the straight-edged maxillary tooth crowns change together along the tree (Fig. 2A). Fig. 8. Evolution of cartilaginous extremity of cervical rib IV. Potential synapomorphy of the Liolaemus darwinii group. Recov- ered Topology of the L. darwinii phylogeny. Liolaemus crepuscula- ris is excluded because of the lack of information on this character state. Fig. 9. Evolution of bladelike process on posterior distal tibia in Liolaemus. Note the secondary loss in the L. anomalus group. Tree modified from Schulte et al. (2000). 73Skeletal variation in the Liolaemus darwinii group This can be interpreted as supporting a possible relation- ship between diet and tooth crown shape. Tooth mor- phology can reflect ecological adaptations and exhibit derived traits which may distinguish alimentary spe- cializations (Hotton, 1965). We found that L. scapula- ris, L. quilmes, L. lavillai, L. grosseorum and L. chacoen- sis have straight crowns and are insectivorous. Lobo and Abdala (2001) cited straight crowns for L. crepuscularis (their L. cf. quilmes). Semhan et al. (2013) reported that L. crepuscularis feeds mainly on insects, but can fluctu- ate to omnivorous or herbivorous diets through the year based on prey availability. All other species studied here show expanded crowns, and their diet can be character- ized as omnivorous or herbivorous (L. albiceps). The only exception to this association is L. koslowskyi, which has expanded crowns and an insectivorous diet (Aun and Martori 1998). Aun and Martori (1998) do not mention the season of the study, so it is not known if this taxon can change its diet as does L. crepuscularis. Phymaturus is the sister clade of Liolaemus, and has a strictly her- bivorous diet (Lobo et al., 2010). Species of Phymaturus have teeth with expanded crowns (Lobo and Quinteros, 2005). The same phenomenon can be observed in non- liolaemid lizards. Hotton (1965) found that herbivorous lizards (Dipsosaurus, Sauromalus, and Ctenosaura) have highly cuspidate and antero-posteriorly widened teeth (similar to the expanded crowns of humans). In lizards that mainly feed on ants (Phrynosoma), Hotton (1965) described pointed and conical teeth, and in lizards that feed on bees and wasps (Urosaurus and Callisaurus), he described thin, cylindrical, sharp teeth. Herrel et al. (2004) observed that lacertids with omnivorous diets show teeth with wider crowns, whereas insectivorous spe- cies had slender and pointed teeth. In agreement with these results, we found that dentition seems to vary with diet. Nevertheless, further studies are needed in Liola- emus in order to confirm the hypothesis of correlation between straight crowns and insectivorous diet. The exposure or not of Meckel’s groove in liolaemid lizards was used by Etheridge (1995) as a character in his taxonomic proposal. He proposed this character as a synapomorphy of the Liolaemus chiliensis (subgenus Liolaemus) group which have a fused channel, and also for the Phymaturus patagonicus group within the sister genus of Liolaemus (Etheridge,1995; Lobo and Quinteros, 2005; Lobo et al., 2010). Lobo and Abdala (2001) viewed the groove as a potential synapomorphy of the L. dar- winii group. Here, we found that Meckel’s groove exhib- its an additional change (reversal), being open in the L. ornatus group. Moreover, Lobo and Abdala (2001) found this character state for L. crepuscularis, a basal member of the L. ornatus group. Therefore, we can conclude that the open Meckel’s groove can be considered a synapomorphy of the L. ornatus group (Fig. 2A-C). The ancestral state would be the open Meckel’s groove, already present in Ctenoblepharys, the basal genus of the Family Liolaemi- dae, and preserved in the Phymaturus palluma group. The hypothesis of the open Meckel’s groove as the ances- tral state is supported by the presence of this character state in other families related to Liolaemidae (e.g., Leio- sauridae and Opluridae according to Pyron et al., 2013 and Reeder et al., 2015), but exhibiting polymorphism in many Iguanian families (Frost and Etheridge, 1989) such as Leiocephalidae (Etheridge, 1966) and Phrynosomati- dae (Etheridge, 1964). The bladelike process on the posterior distal tibia was described by Etheridge (1995), as a synapomorphy of the Liolaemus montanus group. In his proposal, Etheridge (1995) did not include the L. anomalus group inside the L. montanus group. In recent analyses (Espinoza et al., 2004; Abdala 2007), the L. anomalus group is inferred as more closely related to the L. boulengeri group. These two groups together are called the L. boulengeri series (included inside the L. montanus section in Schulte et al., 2000). The L. anomalus group lacks this tibial process, which we consider to be a secondary loss (Fig. 9). Here we found that every member of the L. darwinii group studied has the bladelike process on the distal tibia. Characters that were studied in other groups of liz- ards were informative for Liolaemus, including shape of the premaxilla (Frost, 1992; Tropidurids); squamosal- quadrate joint (modified from Frost, 1992); metacarpal of IV finger reach the I or II phalange of V finger (modified from Arias, 2012; teiids); and presence of open tracheal rings (Lobo and Quinteros, 2005; Lobo et al., 2010; Phy- maturus). Here, we found the same variation in the shape of the premaxilla described by Frost (1992) for tropidurines: (0) narrow nasal spine - wide area of premaxillary tooth attachment and (1) broad nasal spine- narrow premax- illary tooth area. Frost (1992) found no relationship between the width of the area of premaxillary teeth and number of teeth in it. We found similar results for the Liolaemus species studied here, where the number of pre- maxillary teeth is constant regardless of the width of the area in which they are inserted. Also, Frost (1992) described two states for the squa- mosal-quadrate articulation. These states are related to the width of the superior fossa of the quadrate, which may be relatively small or enlarged. In the Liolaemus spe- cies studied, it was observed that the superior fossa of the quadrate corresponds to the “relatively enlarged” state according to Frost (1992). All species except two (L. albi- ceps and L. irregularis) exhibit one of the states proposed 74 L. Díaz-Fernández, A.S. Quinteros, F. Lobo by Frost (1992): the otic ramus of the squamosal contacts (but is not inserted in) the posterior edge of the supe- rior fossa of the quadrate. In contrast, L. albiceps and L. irregularis share the same state, with the otic ramus of the squamosal inserted in the medial part of the fossa (Fig. 6 B), thus reinforcing the hypothesis that they are sister species. The character observed in teiids is related to the rela- tive length of metacarpals, metatarsals and digits. Arias (2012) coded a character related to the length of toe V into three states: length of toe V exceeding that of meta- tarsal IV, equaling that of metatarsal IV, and not reach- ing the length of metatarsal IV. In all Liolaemus species studied here, the toe V always exceeds metatarsal IV in length, but there is variation as to which phalanx of toe V metatarsal IV reaches (Fig. 6 G-H). We recorded this character differently for one of the outgroup taxa (L. inacayali, where metatarsal IV reaches phalanx II) with respect to the L. darwinii group (in which metatarsal IV reaches phalanx III). Since we do not have samples of other members of the L. telsen group, we were not able to determine if this is a potential synapomorphy for that group. Similar variation to that described above for the hindlimbs was described for the forelimbs in Liolaemus. The length of metacarpal IV with respect to finger V, exhibited two states: reaches phalanx I or reaches phalanx II. Within the L. darwinii group, the character state in which finger V reaches phalanx II occurs independently in L. quilmes and L. chacoensis. It is clear that variation between fore and hindlimbs is independent. The presence of intercalated open tracheal rings was only found in the members of the Liolaemus ornatus group. Therefore, this character state can be considered as a possible synapomorphy of this group. Nevertheless, the intercalated open tracheal rings were also found to be polymorphic in L. scapularis and in L. quilmes, spe- cies basal to the L. ornatus group. This character state was primarily proposed for Phymaturus (Lobo and Quinteros, 2005), but no polymorphisms were found in this genus. While newly described characters have not yet been observed in many taxa, a general idea of the polarity of the optimized character was obtained. The study of new sources of variation and the distribution of characters described here in other taxa, as well as in the other two genera of Liolaemidae (Phymaturus and Ctenoblepharys) would allow us to hypothesize about relationships with- in this iguanian family. In this study we note that in the Liolaemus darwinii group the most informative characters are taken from the regions of the ribs, sternal plate, pec- toral girdle, snout, jaw, larynx and hyoid arches. The liter- ature shows that the osteology has been more thoroughly studied in the families Phrynosomatidae, Tropiduridae and Corytophanidae within Iguania (Reeve, 1952; Ether- idge, 1964; Presch, 1969; Etheridge and de Queiroz, 1988; Frost and Etheridge, 1989; Frost, 1992; McGuire, 1996; Reeder and Wiens, 1996; Torrez Carvajal, 2007; Frost et al., 2011) and osteological characters are observed mainly from the cranial skeleton. This shows a bias in the focus of skeletal variation studies, making it difficult to deter- minate the levels of variation across the whole anatomy of lizards. Even if the literature emphasizes the idea of the importance of using osteological characters in different phylogenetic analyses and morphological descriptions (Conrad, 2008; Gauthier et al., 2012; Reeder et al., 2015), it is clear that there is not enough information yet to fully understand the patterns of morphological diversity across all existing iguanian families. ACKNOWLEDGEMENTS We thank Roberto Sanchez, Soledad Valdecantos, Alejandra Paz, Jessica Monroig, Thomas Hibbard, Matias Quipildor, Silvio Salcedo, Sabrina Portelli, Romina Sem- han, Cristian Abdala, Alejandro Laspiur, Adolfo Juarez, Mario Fernandez and Melisa Diaz Fernandez for helping us with field or lab work. Patricia Garcia and T. Hibbard improved the English style. We also thank the Ministe- rio de Ambiente y Producción Sustentable (Secretaria de Ambiente) of Salta Argentina and Y. Bonduri for assist- ing with scientific collection permits (Resolution Nº 815/13). We thank E. Lavilla and S. Kretzschmar (Insti- tuto de Herpetología, Fundación Miguel Lillo, Tucumán Argentina) for allowing us to study FML materials under their care. This study was supported by grants (FL) from CONICET Consejo Nacional de Investigaciones Cientí- ficas y Técnicas of Argentina (PIP 2841) and CIUNSA Consejo de Investigaciones de la Universidad Nacional de Salta, Argentina (CIUNSA 2036), and Graduate Fellow- ship, from Consejo Nacional de Investigaciones Cientí- ficas y Técnicas (LDF). We also thank two anonymous reviewers for their careful reading of our manuscript and their insightful comments and suggestions. REFERENCES Abdala, C.S. (2007): Phylogeny of the boulengeri group (Iguania: Liolaemidae, Liolaemus) based on morpho- logical and molecular characters. Zootaxa 1538: 1-84. Arias, F. (2012): Relaciones filogenéticas en la tribu Teii- ni. (Squamata: Teiidae). Evaluación de la monofilia del género Cnemidophorus y análisis de su estructura 75Skeletal variation in the Liolaemus darwinii group filogenética. Tesis Doctoral. Universidad Nacional de Tucumán, Argentina. Aun, L., Martori, R. 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Liolaemus albiceps (n=4): ARGENTINA: Salta: Los Andes: Camino al Acay desde Estación Muñano, 5-6 km, (24°18'31.6''S; 66°09'7''W) MCN 402, 407, 1452, 1453. Liolaemus chacoensis (n=4): ARGENTINA. MCN 503, 504, 505, 599. No data. Liolaemus grosseorum (n=2): ARGENTINA: Mendo- za: San Rafael: Orillas del embalse el Nihuil, MCN 508, 509. Liolaemus inacayali (n=1): ARGENTINA: Rio Negro: Ing. Jacobacci: 25 de Mayo, MCN 500. Liolaemus irregularis (n=4): ARGENTINA: Salta: Los Andes: Aprox. a 11KM al SE de San Antonio de los Cobres por ruta 51 (24°8'53.44''S; 66°8'21.37''W). MCN 2431, 2436, 2443, 2446. Liolaemus kingii (n=1): ARGENTINA: Santa Cruz. MCN 565. No data. Liolaemus koslowskyi (n=3): ARGENTINA: La Rioja: Castro Barros: 6 km. E. De Anillaco (28°47’S; 66°52’W). MCN 573, 574, 576. Liolaemus lavillai (n=2): ARGENTINA: Jujuy : extremo norte del Parque nacional los cardones, oeste de la Recta de Tin Tin. (25°05’09’’S; 66°00’00’’W). MCN 2688, 4351. Liolaemus multicolor (n=1): ARGENTINA: Jujuy: Abra Pampa: Cochinoca. FML 2065. Liolaemus ornatus (n=4): ARGENTINA: Jujuy: Cas- tro Tolay: A 7 km de S de Rio las Burras. MCN 3545, 3546, 3547, 3548. Liolaemus pseudoanomalus (n=1): ARGENTINA: La Rioja: Castro Barros: 6 km. E. De Anillaco (28°47’S; 66°52’W). MCN 526. Liolaemus quilmes (n=5): ARGENTINA: Salta: Cachi: Cachi: A 7 Km Sur de Palermo entre Cachi adentro y Palermo, (24°58’41,9’’S; 66°08’42,2’’W). MCN 3524, 3525, 3526, 3527, 3528. Liolaemus scapularis (n=2): ARGENTINA: Salta: Cafayate: Los Médanos. MCN 253, 283. Acta Herpetologica Vol. 12, n. 1 - June 2017 Firenze University Press Morphological variation and sexual dimorphism in Liolaemus wiegmannii (Duméril & Bibron, 1837) (Squamata: Liolaemidae) from Uruguay Joaquín Villamil1,*, Arley Camargo2, Raúl Maneyro1 Sex does not affect tail autotomy in lacertid lizards Panayiotis Pafilis1,*, Kostas Sagonas2, Grigoris Kapsalas1, Johannes Foufopoulos3, Efstratios D. Valakos4 Fire salamander (Salamandra salamandra) males’ activity during breeding season: effects of microhabitat features and body size Raoul Manenti*, Andrea Conti, Roberta Pennati Call variation and vocalizations of the stealthy litter frog Ischnocnema abdita (Anura: Brachycephalidae) Pedro Carvalho Rocha1,2,*, João Victor A. Lacerda2,3, Rafael Félix de Magalhães2,3, Clarissa Canedo4, Bruno V. S. Pimenta5, Rodrigo Carrara Heitor6, Paulo Christiano de Anchieta Garcia2,3 Feeding ecology of two sympatric geckos in an urban area of Northeastern Brazil José Guilherme G. Sousa1, Adonias A. Martins Teixeira2,*, Diêgo Alves Teles2, João Antônio Araújo-Filho2 and Robson Waldemar Ávila3 A pattern-based tool for long-term, large-sample capture-mark-recapture studies of fire salamanders Salamandra species (Amphibia: Urodela: Salamandridae) Jeroen Speybroeck1,*, Koen Steenhoudt2,$ Skeletal variation within the darwinii group of Liolaemus (Iguania: Liolaemidae): new characters, identification of polymorphisms and new synapomorphies for subclades Linda Díaz-Fernández*, Andrés S. Quinteros, Fernando Lobo Marking techniques in the Marbled Newt (Triturus marmoratus): PIT-Tag and tracking device implant protocols Hugo Le Chevalier1, Olivier Calvez2, Albert Martínez-Silvestre3, Damien Picard4, Sandra Guérin4,5, Francis Isselin-Nondedeu6, Alexandre Ribéron1, Audrey Trochet1,2,* Evidence for directional testes asymmetry in Hyla gongshanensis jindongensis Qing Gui Wu1, Wen Bo Liao2,* The advertisement call of Pristimantis subsigillatus (Anura, Craugastoridae) Florina Stănescu1,4, Rafael Márquez2, Paul Székely3,4,*, Dan Cogălniceanu1,4,5 Nematodes Infecting Anotosaura vanzolinia (Squamata: Gymnophthalmidae) from Caatinga, northeastern Brazil Bruno Halluan S. Oliveira¹, Adonias A. Martins Teixeira¹,*, Romilda Narciza M. Queiroz¹, João A. Araujo-Filho¹, Diêgo A. Teles¹, Samuel V. Brito2, Daniel O. Mesquita¹ Where is my place? Quick chorus structure assembly in the European tree frog Michal Berec A possible mutualistic interaction between vertebrates: frogs use water buffaloes as a foraging place Piotr Zduniak1,*, Kiraz Erciyas-Yavuz2, Piotr Tryjanowski3 Good vibrations: A novel method for sexing turtles Donald T. McKnight1,2,*, Hunter J. Howell3, Ethan C. Hollender1, and Day B. Ligon1 Errata to Mecke, S., Hartmann, L., Mader, F., Kieckbusch, M., Kaiser, H. (2016): Redescription of Cyrtodactylus fumosus (Müller, 1895) (Reptilia: Squamata: Gekkonidae), with a revised identification key to the bent-toed geckos of Sulawesi. Acta Herpetologica 11(2):