New record of Phytophthora root and stem rot of Lavendula angustifolia LESZEK B.ORLIKOWSKI1 and ALMA VALJUSKAITE2 1Research Institute of Pomology and Floriculture Pomologiczna 18, PL-96-100 Skierniewice, lorlikow@insad.pl 2Lithuanian Institute of Horticulture, Plant Protection Laboratory Babtai, Kauno 30, LT-54333 O r l i k o w s k i L.B., Va l j u s k a i t e A.: New record of Phytophthora root and stem rot of Lavendula angustifolia. Acta Mycol. 42 (2):193-198, 2007. Phytophthora cinnamomi was isolated from rotted root and stem parts of lavender as well as from soil taken from containers with diseased plants. Additionally Botrytis cinerea, Fusarium spp. and Sclerotinia sclerotiorum were often isolated from diseased tissues. P. cinnamomi colonised leaves and stem parts of 4 lavender species in laboratory trials and caused stem rot of plants in greenhouse experiments. Cardinal temperature for in vitro growth were about 7,5 and 32°C with optimum 25-27,5°C. The species colonised stem tissues at temperature ranged from 10° to 32°C. Key words: stem rot, isolation, Phytophthora cinnamomi, colonisation, temperature, growth INTRODUCTION Lavender (Lavendula angustifolia Mill., syn. L.officinalis Chaix) is main- ly medicinal and pharmaceutic plant but since the last years it has been grown in ornamental nurseries mainly as small, blooming, hedge culture. The quality of plants may be strongly decreased by Phytophthora species. From diseased plants mainly Phytophthora nicotianae var. parasitica was isolated (P a p p a s 1978; P u t m a n 1991; M i n u t o et al. 2001a). Growing of plants in Italy un- der shade and in larger pots reduced disease incidence caused by that species (M i n u t o et al. 2001a). Variable results were obtained with susceptibility of cultivars or local selections toward the pathogen (M i n u t o et al. 2001b). P a e z et al. (1993) recovered P. palmivora Butler from diseased Lavendula dentata grown in Spanish gardens whereas D a v i n o et al. (2002) isolated the species from rotted root of L. angustifolia in Italy. In 2003 on lavender mother plants grown on greenhouse beds yellowing and browning of single shoots were observed. The disease spread slowly on neighbouring ACTA MYCOLOGICA Vol. 42 (2): 193-198 2007 Dedicated to Professor Alina Skirgiełło on the occasion of her ninety-fifth birthday 194 L.B. Orlikowski and A. Valjuskaite shoots. After the next 6 weeks 3/4 parts of affected plants were destroyed (Fig.1). On mother plants dark-gray spots on the shoot bases, browning during the next few days and spreading on roots, upwards and on the periphery of affected stems were observed. The disease spread quickly on neighbouring mother plants and cuttings. On plants growing in container - grown nursery brown or dark-brown spots devel- oped on individual shoots spread on roots and upwards. During the next 10 days single or a few yellow-brown to dark-brown shoots on individual plants were seen. The development of symptoms were observed during 2 years before or during flow- ering of plants. The objective of this investigation was to determine a causal agent of lavender shoot and root rot, development of disease in laboratory and greenhouse trials and relationship between temperature and growth of Phytophthora cinnamomi and colonisation of stem parts of plants. MATERIALS AND METHODS Survey of lavender in greenhouse and container - grown nursery. On plants grown in greenhouse shoot rot symptoms were observed only in one year on about 5% of lavender. In container - grown nursery lavender were grown at least 10 years but shoot and root rot symptoms occurred in years 2004 and 2005 on about 10 and 20%,respectively. Diseased plants were collected in plastic bags and transferred to laboratory. The next day plants were removed from pots, substratum were collected in bags whereas plants were washed under tap water and affected shoots were sepa- rated, rinsed 3times in distilled water, blotting dried and chosen parts were sterilised over a burner flame. About 5 mm long stem parts were put on PDA medium and plates were checked during 4-day-incubation at 24°C in the dark on the presence of Phytophthora and other genera. Additionally, substratum samples, taken from pots with lavender showing disease symptoms in greenhouse and nursery, were analysed on the presence of Phytophthora spp. in years 2003-2005. Substratum taken from 3-6 pots was mixed about 5 min in plastic bag and half liter of it was put into plastic box, flooded with tap water (about 1 l) and rhododendron leaves cv. Zembla were floated over flooded sample (16 leaf blades/box). Boxes covered with plastic foil were incu- bated at temperature 22-24°C. After 4-6-day-incubation leaves with necrotic spots were removed, washed with distilled water, blotted dried, disinfected over a burner flame and about 5 mm diameter plugs were transferred on PDA medium. Small parts of colonies, grown around tissues were transferred to PDA slants. Cultures from dis- eased stems and substratum were grouped on the base of their growth and morpholo- gy and chosen, representative isolates were identified to genera and species (S z k u t a 2004). Confirmation of Phytophthora classification to species was performed by DNA analyses using the method described by O r l i k o w s k i et al. (2006). Colonisation of lavender by Phytophthora cinnamomi. In laboratory trials leaves and stem parts of 4 cultivars of lavender (Tab. 1) were put into plastic boxes on sterilised, wet blotting paper covered with plastic net and inoculated with 3mm plugs taken from the edge of 5-day-old cultures of P. cinnamomi (2 isolates from diseased stem base and substratum) growing on PDA at 24°C in the dark. Boxes were covered with foil and incubated at 22-24°C in the dark. After 5-day-incubation diameter and length of necrosis was measured. In greenhouse trial stem bases of 3 cultivars of lav- ender were inoculated with 3 mm diam plugs of P. cinnamomi (from diseased stem New record of Phytophthora 195 base) and incubated under covering 12 days. Length of necrosis was measured 8 and 12 days after inoculation. Relationship between temperature, ranged from 7,5 to 35°C, growth of P. cin- namomi and colonisation of lavender stem parts by the species were studied using the same procedure like in laboratory trials. Experimental design was completely randomised with 4 replications and 10 leaves, stem parts or plants in each rep. Growth of the species on PDA was esti- mated on the base of 5 Petri dishes for each temperature. Trials were repeated at least twice. RESULTS AND DISCUSSION Isolation of fungi and Algae-like Oomycetes. Similar genera and species were iso- lated from plants taken from greenhouse and field production (Tab. 2). From potential Ta b l e 1 Phytophthora cinnamomi isolated from substratum used for lavender growth; mean number of dark-brown spots on rhododendron leaves used as the bait Source of substratum Year of analysis 2003 2004 2005 Greenhouse 25.5 b - - Container nursery 14.0 a 10.8 a 17.6 ab Note: Means followed by the same letter do not differ with 5% of significance (Duncan’s multiple range test) Ta b l e 2 Fungi and Algae-like Oomycetes isolated from diseased base of Lavendula angustifolia; number of settled plants (a) and number of isolates obtained (b) Genera/species Year of isolation 2003 greenhouse (27 plants) 2004 field (14 plants) 2005 field (21 plants) a b a b a b Alternaria alternata Nees 2 5 6 11 8 13 Botrytis cinerea Pers. 7 11 4 9 5 11 Cladosporium herbarum (Pers.) Link. 3 3 - - 2 4 Fusarium culmorum (W.G.Sm.) Sacc. 4 7 2 5 - - Fusarium equiseti (Cda) Sacc. 3 5 - - 3 7 Fusarium solani (Mart.) Sny. et Hans. 6 7 3 5 1 3 Humicola grisea Traaen 1 2 - - 2 5 Mucor spp. 4 8 5 9 7 16 Penicillium spp. 3 5 2 4 - - Phytophthora cinnamomi Rands 18 56 9 29 18 44 Rhizopus sp. - - 2 4 - - Sclerotinia sclerotiorum (Lib.) de Bary 4 10 - - 2 7 Trichoderma spp. 3 6 3 7 4 7 196 L.B. Orlikowski and A. Valjuskaite pathogens of lavender Botrytis cinerea (mainly in field), two Fusarium species, Phytoph- thora cinnamomi and Sclerotinia sclerotiorum were detected from diseased plant parts. P. cinnamomi (Fig.2) was the most often isolated species both, in greenhouse and field - grown plants. The species was detected from 3/4, 5/7 and 6/7 of analysed plants, respectively (Tab. 2). Isolation of Phytophthora cinnamomi from substratum. Using rhododendron leaves as the bait, P. cinnamomi was detected from substratum samples taken from 60 pots with plants showing Phytophthora rot symptoms. Significantly more necrotic spots were observed on rhododendron leaves floated in suspension of substratum collected from diseased lavender grown in greenhouse (Tab. 1). Colonisation of lavender by Phytophthora cinnamomi. Both isolates of P. cinnamomi used for lavender inoculation in laboratory trials, caused necrosis of leaves and stem parts of 4 tested cultivars (Tab. 3). On stem parts necrosis spread faster than on leaves. In case of leaf blades inoculation, isolate from substratum colonised tissue slower than that from the stem base. On leaves of cv. Grosso necro- sis spread at least 1/3 faster than on 3 other cultivars. On stem parts such differences were not observed (Tab. 3). Ta b l e 3 Colonisation of leaves and stem parts of lavender by Phytophthora cinnamomi; diam/length (in mm) of necrosis after 5-day-incubation Inoculation: 2005.09.27 Source of P. cinnamomi Cultivars Leaves Stem parts Base of stem Blue Dafo 32.3 ab 43.3 a Edelweiss 35.0 bc 40.3 a Grosso 32.0 a 39.5 a Munstead 35.5 c 38.8 a Substratum Blue Dafo 19.5 b 46.3 b Edelweiss 15.3 a 38.8 a Grosso 30.8 c 45.3 ab Munstead 22.5 b 38.3 a Note: Means in columns followed by the same letter do not differ with 5% of significance; means separation for the source of the species Ta b l e 4 Spread of necrosis (in mm) on lavender stems inoculated with Phytophthora cinnamomi from the stem base in relation to cultivars and incubation time; greenhouse trials Inoculation: 2005.11 17 Cultivars Length of necrosis in mm after days of incubation 8 12 Blue Dafo 66.5 b 87.6 b Grosso 53.4 a 73.7 ab Munstead 48.7 a 65.3 a Note: see Table 3 198 L.B. Orlikowski and A. Valjuskaite SUMMARY The objective of this investigation was to determine a causal agent of lavender shoot and root rot, development of disease on plant parts and relationship between temperature and growth of pathogen and disease development. During 3 years my- cological analyse of diseased lavender, taken from greenhouse and container nurs- ery, was done. Additionally, the presence of P. cinnamomi in substratum was esti- mated using rhododendron leaves as the bait. Phytophthora cinnamomi was isolated from the most of analysed plants and from substratum. Botrytis cinerea, Fusarium spp. and Sclerotinia sclerotiorum as other potential pathogens, were recovered from diseased shoot parts. Isolates of P. cinnamomi from plant and substratum colonised lavender leaves and stem parts of all tested cultivars. In greenhouse experiment the species quickly colonised stem tissues and necrosis spread about 5,6 mm/24 hr. The growth of the pathogen on PDA and colonisation of stem parts were observed at temperature range from about 7,5 to 32,5 °C with optimum 25 -27,5°C. REFERENCES D a v i n o S., C a c c i o l a S. O., P e n n i s i A. M., D e s t r i N i c o s i a M. G. 2002. Phytophthora palmivora a new pathogen of lavender in Italy. Plant Disease 86 (5): 561. M i n u t o A., G u l l i n o M. L., T i t o n e P., G a r i b a l d i A. 2001. Influence of cultural practices on inci- dence of Phytophthora nicotianae var. parasitica causing root rot of lavender (Lavendula officinalis L.). Phytopathol. Meditterranea 40 (1): 45–54. M i n u t o G., A r m a t o B., G i l a r d i G., G a r i b a l d i A. 2001. First observations on susceptibility of Lavendula spp. to Phytophthora nicotianae var. parasitica. Informatore Fitopatologico 51 (5):69–72. O r l i k o w s k i L.B., Tr z e w i k A., W i e j a c h a K. 2006. Phytophthora tropicalis, a new pathogen of orna- mental plants in Poland. J. Plant Prot. Res. (in print). P a e z J. I., B e r r a D., Ve g a J. M., Te l l o J. 1993. Identification de Phytophthora palmivora Butler en jardines de la Exposicion Universal de Sevilla. Bol. de Sanidaad Vegetal, Plagas 19 (4): 633–447. P a p p a s A. 1978. New diseases caused by Phytophthora spp. Phytophthora Newsl. 6: 72–73. P u t n a m M. 1991. Root rot of lavender caused by Phytophthora nicotianae. Plant Pathol. 40: 480–482. Występowanie zgnilizny korzeni i pędów, powodowanej przez Phytophthora cinnamomi, na Lavendula angustifolia S t r e s z c z e n i e Celem badań było określenie przyczyny zgnilizny pędów i korzeni lawendy, rozwo- ju choroby w doświadczeniach laboratoryjnych i szklarniowych oraz współzależności po- między temperaturą a wzrostem in vitro patogena oraz kolonizacją części łodyg. W ciągu 3 lat prowadzono analizę mikologiczną chorych roślin lawendy, pobieranych ze szklarni oraz ze szkółki pojemnikowej. Dodatkowo, stosując pułapki z liści różanecznika izolowa- no Phytophthora spp. z podłoża. Z większości chorych roślin, a także z podłoża izolowano Phytophthora cinnamomi. Z innych organizmów izolowano Botrytis cinerea, Fusarium spp. i Sclerotinia sclerotiorum. Izolaty P. cinnamomi z porażonej rośliny i podłoża kolonizowały liście i części łodyg wszystkich badanych odmian lawendy. W doświadczeniu szklarniowym omawiany gatunek szybko kolonizował tkanki pędów, a nekroza rozwijała się ok. 5,6 mm/ dobę. Wzrost P. cinnamomi na pożywce PDA oraz kolonizację pędów obserwowano w tempe- raturze od 7,5° do 32,5°C z optimum 25-27,5°C. Fig. 2. Zoosporangia of Phytophthora cinnamomi and released zoospores. Phot. G. Szkuta. Fig.1. Phytophthora shoot and stem rot of lavender cuttings. Phot. L. B. Orlikowski. 2014-01-01T11:46:10+0100 Polish Botanical Society