Armillaria species in coniferous stands ANNA ŻÓŁCIAK Forest Research Institute, Sękocin Stary, Braci Leśnej 3, PL-05-090 Raszyn, A.Zolciak@ibles.waw.pl Ż ó ł c i a k A.: Armillaria species in coniferous stands. Acta Mycol. 42 (2):211-217, 2007. Identification of the Armillaria species in selected coniferous stands (Scots pine stands, Norway spruce stands and fir stands) was the aim of the work carried out on the basis of mating tests and consideration of macroscopic traits of fruit bodies. One species of Armillaria [A. ostoyae (Romagnesi) Herink] was found in Scots pine stands, three species [A. ostoyae, A. cepistipes Velenovský and A. borealis Marxmüller et Korhonen] were found in Norway spruce stands and two species [A. ostoyae and A. cepistipes] were found in fir stands. Key words: coniferous stands, Armillaria ostoyae, A. cepistipes, A. borealis INTRODUCTION Armillaria root infection is the most important disease affecting mainly young Scots pines (Pinus sylvestris L.) planted often in/after mixed stands in the north-east part of Poland and Norway spruce (Picea abies Karst.) stands in the south part of the country (M a ń k a 1953, 1998; Tw a r o w s k i , Tw a r o w s k a 1959; Tw a r o w s k a 1965; R y k o w s k i 1985; C a p e c k i 1994, 1997; S i e r o t a 2001; L e c h 2003; M a ń k a et al. 2003). It is caused by Armillaria species. From seven European Armillaria species, five were identified in Poland: A. borealis Marxmüller et Korhonen, A. cepistipes Vele- novský, A. ostoyae (Romagn.) Herink, A. mellea (Vahl: Fr.) Kummer and A. gallica Marxmüller et Romagnesi (Ż ó ł c i a k 1999). The objectives of the present study were to identify Armillaria species in selected coniferous stands: Scots pine stands, Norway spruce stands and fir stands in Poland and to determine the occurrence of Armillaria species considering forest habitat (according to Polish forest site typology) and stand age classes. MATERIALS AND METHODS Material (samples) consisted of fruit bodies of Armillaria, fragments of wood colonized by mycelium of Armillaria and rhizomorphs. Samples were taken from ex- perimental one-time-sampled plots of 500 m2 each (20x25 m) established in produc- tion stands: Scots pine stands, Norway spruce stands and fir stands. The stands were ACTA MYCOLOGICA Vol. 42 (2): 211-217 2007 Dedicated to Professor Alina Skirgiełło on the occasion of her ninety-fifth birthday 212 A. Żółciak chosen on the basis of data obtained from questionnaires of the annual forest state assessments informing about the area of stands infested by root rot of Armillaria, inventory documents and the author’s own observations. One hundred and five plots were established in the Scots pine stands, sit- uated within the territory of 52 Forest Districts (Fig. 1A). On each plot 1 to 10 samples were taken in 1985, 1989-94 and 1996-1998. Fifty plots were established in the Norway spruce stands, situated within the territory of 17 Forest Districts, 1 - in Gorce National Park and 1 - in Krynica Experimental Forest. On each plot 1 to 15 samples were taken in 1981, 1989, 1991, 1994, 1996-98 and 2001-03. Fifteen plots were establish in the fir stands, situated within the territory of 5 Forest District, 1 – in Gorce National Park and 1 – in Rotocze National Park. On each plot 1-12 samples were taken in 1991, 1994, 1996 and 1998. Characterization of the stands and samples is presented in table 1. The identification of Armillaria isolates was performed by using mating tests (K o r h o n e n 1978). Fruit bodies of Armillaria were identified on the basis of the macro- and microscopic features (R o m a g n e s i , M a r x m ü l l e r 1983). Ta b l e 1 Characterization of stands, from which samples of Armillaria were taken Stands Age of stands Type of forest habitat* Proportion of main species (%) Number of the experi- mental plots samples obtained isolates (fruit bodies/ colonized wood/ rhizomorphs) identified using mating tests samples (fruit bodies) identified of the macro- and micro- scopic features Scots pine 1-160 FCF, FMCF, FMBF, MMCF, MMBF 100 (Scots pine) 105 356 176 (71/105/-) 180 Norway spruce 1-120 FMCF, FBF, FMBF, MUF, MMF, MF 60-100 Norway spruce 50 228 114 (86/27/1) 114 fir 41-140 FBF, UF, MMF, MF 50-100 (fir) 15 53 25 (23/2/-) 28 Total 170 637 315 (180/134/1) 322 Explanations: fresh coniferous forest (FCF), fresh mixed coniferous forest (FMCF), moist mixed conifer- ous forest (MMCF), fresh broadleaved forest (FBF), fresh mixed broadleaved forest (FMBF), upland forest (UF), mixed upland forest (MUF), moist mixed broadleaved forest (MMBF), mountain forest (MF), mixed mountain forest (MMF). Armillaria species 213 Fig. 1. A) Distribution of experimental plots. B) Distribution of Armillaria species in Scots pine stands, Norway spruce stands and fir stands. 214 A. Żółciak RESULTS A total of 637 samples were collected in the investigated coniferous stands (356 samples - in Scots pine stands, 228 samples - in Norway spruce stands and 53 samples – in fir stands). The proportions of Armillaria samples found in the coniferous stands were calculated (Tabs 2 and 3). Ta b l e 2 Percentage of samples of Armillaria from coniferous stands: Scots pine, Norway spruce and fir considering different types of forest habitat Stands Type of forest habitat FCF (%) FMCF (%) MMCF (%) FBF (%) FMBF (%) MMBF (%) UF (%) MUF (%) MMF (%) MF (%) Scots pine 9,3 78,4 5,6 0,8 4,8 1,1 - - - - Norway spruce 0,9 - - 2,6 12,7 - - 13,6 36,4 33,8 Fir - - - 3,8 - - 45,3 - 3,8 47,1 Ta b l e 3 Percentage of samples of Armillaria from coniferous stands: Scots pine stands, Norway spruce stands and fir stands considering different stand age classes Stands Stand age classes 1-20 (%) 21-40 (%) 41-60 (%) 61-80 (%) 81-100 (%) 101-120 (%) 121-140 (%) 141-160 (%) Scots pine 84,8 0,6 1,7 7,0 2,2 2,8 0,6 0,3 Norway spruce 2,2 1,3 38,6 45,6 11,4 0,9 - - Fir - - 5,7 7,5 26,4 37,7 22,6 - Only one species of Armillaria – A. ostoyae was found in the Scots pine stands, three species: A. ostoyae, A. cepistipes and A. borealis were found in the Norway spruce stands and two species: A. ostoyae and A. cepistipes were found in the fir stands (Fig. 1B). In the Norway spruce stands, among the identified isolates and fruit bodies belonging to genus Armillaria, the species A. ostoyae attained the high- est proportion – 85,5%. The remaining ones were less frequent: A. borealis – 11,4% and A. cepistipes – 3,1%. In the fir stands share of A. ostoyae was 51,2% and share of A. cepistipes was 48,8%. In the Scots pine stands the species A. ostoyae was found in six forest site types, mainly in fresh mixed coniferous forest (Tab. 4). In the Norway spruce stands all the three identified species were found in fresh mixed broadleaved forest site type. The species A. ostoyae was recorded in six investigated site types. A. borealis was found in fresh broadleaved forest site type and fresh mixed broad- leaved forest site type. A. cepistipes was found in fresh mixed broadleaved forest site type, mixed mountain forest site type, and mountain forest site type. In the fir stands A. ostoyae was noticed on three forest site types: upland forest, mountain forest and mixed mountain forest, A. cepistipes – in fresh broadleaved forest, upland forest, and mountain forest. Armillaria species 215 Ta b l e 4 Percentage of particular Armillaria species in samples from coniferous stands from different types of forest habitat Type of forest habitat Species of Armillaria A. borealis (%) A. cepistipes (%) A. ostoyae (%) Scots pine stands FCF - - 9,3 FMCF - - 78,4 MMCF - - 5,6 FBF - - 0,8 FMBF - - 4,8 MMBF - - 1,1 Norway spruce stands FMCF - 28,6 0,9 FBF 3,8 - 2,6 FMBF 96,2 - 12,7 MUF - - 13,6 MMF - 57,1 36,4 MF - 14,3 33,8 fir stands FBF - 9,5 - UF - 38,1 50,0 MMF - - 6,2 MF` - 52,4 43,8 Ta b l e 5 Percentage of particular Armillaria species in samples from coniferous stands of different stand age classes Stand age classes Species of Armillaria A. borealis (%) A. cepistipes (%) A. ostoyae (%) Scots pine stands 1-20 - - 84,8 21-40 - - 0,6 41-60 - - 1,7 61-80 - - 7,0 81-100 - - 2,2 101-120 - - 2,8 121-140 - - 0,6 141-160 - - 0,3 Norway spruce stands 1-20 - - 2,2 21-40 - - 0,9 41-60 88,5 57,1 8,8 61-80 3,8 14,3 75,4 81-100 7,7 28,6 8,3 101-120 - - 4,4 fir stands 41-60 - - 9,4 61-80 - 9,5 6,2 81-100 - 23,8 28,1 101-120 - 42,9 31,3 121-140 - 23,8 25,0 216 A. Żółciak A. ostoyae was found most frequently in the 1-20-year-old Scots pine stands (84,8%), in the 61-80-year-old Norway spruce stands (75,4%) and in the 101-120- year-old fir stands (31,3%; Tab. 5). A. borealis and A. cepistipes were noticed mainly in the 41-60-year-old Norway spruce stands. A. cepistipes was collected particularly in the 101-120-year-old fir stands. CONCLUSIONS A. ostoyae seems to be the most frequent species of Armillaria in coniferous stands in Poland. It was found in the all investigated stands (100% in pine, 85,5% in Nor- way spruce and 51,2% in fir stands), in all types of forest habitat and in all stand age classes. It is in concordance with findings of many authors that A. ostoyae is respon- sible for most cases of Armillaria attacks in conifers (G u i l l a u m i n , B e r t h e l a y 1981; R i s h b e t h 1982; R o l l -H a n s e n 1985; G u i l l a u m i n et al 1985; R y k o w s k i 1990; G u i l l a u m i n et al 1993). A. borealis was found only in the older (41-100-years-old) Norway spruce stands in fertile forest site types (FBF, FMBF). A. cepistipes was noticed in the older Nor- way spruce and fir stands and in many forest site types except for weak coniferous ones. Over 50% of samples of that species were collected in the mountain sites. Acknowledgement. I gratefully acknowledge Dr Kari Korhonen from the Finnish Research Institute for the testers of Armillaria. REFERENCES C a p e c k i Z. 1994. Rejony zdrowotności lasów zachodniej części Karpat. (Forest health condition re-Rejony zdrowotności lasów zachodniej części Karpat. (Forest health condition re- gions in western Carpathians). Prace Inst. Bad. Leśn. A, 781: 83–191. C a p e c k i Z. 1997. Rejonizacja zdrowotności lasów środkowej części Karpat. (Regions of differing health in the central part of the Carpathians). Prace Inst. Bad. Leśn. A, 840: 83–191. G u i l l a u m i n J. J., B e r t h e l a y S. 1981. Détermination spécifique des armillaires par la méthode des groupes de compatibilité sexuelle. Spécialisation écologique des espèces françaises. Agronomie 1: 897–908. G u i l l a u m i n J. J., L u n g B., R o m a g n e s i H., M a r x m ü l l e r H., L a m o u r e D., D u r r i e u G., - J. J., L u n g B., R o m a g n e s i H., M a r x m ü l l e r H., L a m o u r e D., D u r r i e u G., -L u n g B., R o m a g n e s i H., M a r x m ü l l e r H., L a m o u r e D., D u r r i e u G., - B., R o m a g n e s i H., M a r x m ü l l e r H., L a m o u r e D., D u r r i e u G., -R o m a g n e s i H., M a r x m ü l l e r H., L a m o u r e D., D u r r i e u G., - H., M a r x m ü l l e r H., L a m o u r e D., D u r r i e u G., -M a r x m ü l l e r H., L a m o u r e D., D u r r i e u G., - H., L a m o u r e D., D u r r i e u G., -L a m o u r e D., D u r r i e u G., - D., D u r r i e u G., -D u r r i e u G., - G., -B e r - t h e l a y S., M o h a m e d C. 1985. Systématique des Armillaires du groupe Mellea. Conséquences phytopathologiques. Eur. J. For. Path. 15: 268–277. G u i l l a u m i n J. J., M o h a m e d C., A n s e l m i N., C o u r t e c u i s s e R., G r e g o r y O., r - J. J., M o h a m e d C., A n s e l m i N., C o u r t e c u i s s e R., G r e g o r y O., r -M o h a m e d C., A n s e l m i N., C o u r t e c u i s s e R., G r e g o r y O., r - C., A n s e l m i N., C o u r t e c u i s s e R., G r e g o r y O., r -A n s e l m i N., C o u r t e c u i s s e R., G r e g o r y O., r - N., C o u r t e c u i s s e R., G r e g o r y O., r -C o u r t e c u i s s e R., G r e g o r y O., r - R., G r e g o r y O., r -G r e g o r y O., r - O., r -H o l d e n r i e d - e r O., I n t i n i M., L u n g B., M a r x m ü l l e r H., M o r r i s o n D., R i s h b e t h J., Te r m o r s h u i z e n A. J., T i r r ó A., Va n D a m B. 1993. Geographical distribution and ecology of the Armillaria species in western Europe. Eur. J. For. Path. 23: 321–341. K o r h o n e n K. 1978. Interfertility and clonal size in the Armillaria mellea complex. Karstenia 18: 31-42. L e c h P. 2003. Zagrożenie drzewostanów świerkowych w Polsce przez patogeny korzeni w świetle wyn- ików monitoringu fitopatologicznego lasów gospodarczych. (In:) A. G r z y w a c z (ed.). Drzewosta- ny świerkowe – stan, problemy, perspektywy rozwojowe. Materiały Sesji Naukowej PTL, Ustroń- Jaszowiec: 92–107. M a ń k a K. 1953. Badania terenowe i laboratoryjne nad opieńką miodową – Armillaria mellea (Vahl) Quél. Prace Inst. Bad. Leśn. 94: 1–96. M a ń k a K. 1998. Fitopatologia leśna. PWRiL. Warszawa. 368 pp. M a ń k a M., P r z y b y ł K., M a ł e c k a M. 2003. Choroby świerka na tle zmian środowiska. (In:) materiałach Sesji Naukowej PTL, Drzewostany świerkowe: stan, problemy, perspektywy rozwojowe. Ustroń-Jaszowiec: 63–76. R i s h b e t h J. 1982. Species of Armillaria in southern England. Pl. Path. 31: 9–17. R o l l -H a n s e n F. 1985. The Armillaria species in Europe. Eur. J. For. Path. 15: 22–31. Armillaria species 217 R o m a g n e s i H., M a r x m ü l l e r H. 1983. Etude complémentaire sur les armillaires annelées. Bull. Soc. Mycolog. de France 99: 301– 324. R y k o w s k i K. 1985. Niektóre troficzne uwarunkowania patogeniczności Armillaria mellea (Vahl) Quèl. w uprawach sosnowych. Prace Inst. Bad. Leśn. 640: 1–140. R y k o w s k i K. 1990. Opieńkowa zgnilizna korzeni. PWRiL. Warszawa. 16 pp. S i e r o t a Z. 2001. Choroby lasu. Centrum Informacyjne Lasów Państwowych. 156 pp. Tw a r o w s k a I. 1965. Opieńka miodowa. PWRiL Warszawa. 54 pp. Tw a r o w s k i Z., Tw a r o w s k a I. 1959. Studies and observations on Armillaria mellea (Vahl) Quél. as the cause of mass dying-off of forest stands. Prace Inst. Bad. Leśn. 192: 1–61. Ż ó ł c i a k A. 1999. Identyfikacja gatunków grzybów z rodzaju Armillaria (Fr.: Fr.) Staude w Polsce. Prace Inst. Bad. Leśn. 888: 3–19. Opieńki w drzewostanach iglastych S t r e s z c z e n i e Materiał badawczy (owocniki, fragmenty drewna przerośniętego grzybnią, ryzomorfy) pobierano w różnych latach, na jednorazowych powierzchniach w wybranych drzewostanach iglastych: sosnowych, świerkowych i jodłowych. Izolaty identyfikowano za pomocą testów in- tersterylności. Owocniki identyfikowano na podstawie cech makroskopowych i mikroskopo- wych. Wśród badanych próbek stwierdzono trzy gatunki opieniek. Stwierdzono w drzewosta- nach: sosnowych – A. ostoyae, świerkowych – A. ostoyae, A. borealis i A. cepistipes, jodłowych – A. ostoyae i A. cepistipes. A. ostoyae wydaje się być jednym z najczęściej występujących gatun- ków opieniek w drzewostanach iglastych w Polsce. Stwierdzono ten gatunek we wszystkich ba- danych drzewostanach iglastych. Jego udział stanowił 100% prób zebranych w drzewostanach sosnowych, 85,5% – w drzewostanach świerkowych oraz 51,5% – w drzewostanach jodłowych. A. ostoyae stwierdzono na wszystkich typach siedliskowych lasu i w drzewostanach we wszyst- kich klasach wieku. A. borealis stwierdzono tylko w drzewostanach świerkowych, w wieku 41-100 lat, na bogatych siedliskach. A. cepistipes stwierdzono w drzewostanach świerkowych i jodłowych, w starszym wieku, na większości badanych siedlisk. 2014-01-01T11:46:21+0100 Polish Botanical Society