Voltammetric determination of vitamin B6 in the presence of vitamin C based on zinc ferrite nanoparticles modified screen-printed graphite electrode doi: https://doi.org/10.5599/admet.1702 251 ADMET & DMPK 11(2) (2023) 251-261; doi: https://doi.org/10.5599/admet.1702 Open Access : ISSN : 1848-7718 http://www.pub.iapchem.org/ojs/index.php/admet/index Original scientific paper Voltammetric determination of vitamin B6 in the presence of vitamin C based on zinc ferrite nano-particles modified screen- printed graphite electrode Peyman Mohammadzadeh Jahani1, Maedeh Jafari2 and Sayed Ali Ahmadi*3 1 School of Medicine, Bam University of Medical Sciences, Bam, Iran 2 Department of Pediatrics, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran 3 Department of Chemistry, Kerman Branch, Islamic Azad University, Kerman, Iran *Corresponding Author: E-mail: ahmadi.iauk59@gmail.com Received: February 02, 2023; Revised: February 23, 2023; Published: March 15, 2023 Abstract The zinc ferrite nano-particles (ZnFe2O4) modified screen-printed graphite electrode (ZnFe2O4/SPGE) was used for the voltammetric determination of vitamin B6 in real samples, using differential pulse voltammetry (DPV). It has been found that the oxidation of vitamin B6 at the surface of such an electrode occurs at a potential about 150 mV less positive compared to an unmodified screen-printed graphite electrode. After optimization, a vitamin B6 sensor with a linear range from 0.8 to 585.0 µM and a detection limit of 0.17 µM. The ZnFe2O4/SPGE sensor exhibits good resolution between the voltammetric peaks of vitamin B 6 and vitamin C, making it suitable for detecting vitamin B6 in the presence of vitamin C in real samples. ©2023 by the authors. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). Keywords Zinc ferrite nanoparticles; voltammetry; vitamin B6; vitamin C; modified electrode Introduction Vitamins are small organic molecules whose lack or excess may result in several diseases to the organisms that need them. They are classified into two groups by their solubilities, namely water-soluble vitamins (vitamin B and vitamin C) and fat-soluble vitamins (vitamin A, vitamin D, vitamin E, and vitamin K) [1 2]. Vitamin B6 belongs to the water-soluble B complex vitamins group, commonly called pyridoxine. It is essential in the diet for the metabolism of amino acids and the maintenance of body cells. The nervous and immune systems need vitamin B6 for efficient functioning; it also plays a major role in the conversion of tryptophan to niacin [3,4]. Also, It is found in different chemical forms (pyridoxamine, pyridoxine or 5-phos- phate derivatives), but the most stable form is pyridoxine which is used in drug formulations such as multivitamin supplements or in enriched foods [5]. Vitamin C is one of the most important water-soluble vitamins and it refers to all compounds exhibiting equivalent biological activity to L-ascorbic acid (AA), including dehydroascorbic acid (DHAA), the oxidation product of AA, its isomers and esters. Vitamin C is an antioxidant necessary for the growth, development, and repair of all tissues [6,7]. Low levels of vitamin C can result in a condition called scurvy. Scurvy may cause https://doi.org/10.5599/admet.1702 https://doi.org/10.5599/admet.1702 http://www.pub.iapchem.org/ojs/index.php/admet/index mailto:ahmadi.iauk59@gmail.com http://creativecommons.org/licenses/by/4.0/ P. M. Jahani et al. ADMET & DMPK 11(2) (2023) 251-261 252 symptoms such as rash, muscle weakness, joint pain, tiredness, or tooth loss. Vitamin C is an important antioxidant, along with vitamin E, beta-carotene, and many other plant-based nutrients. Antioxidants block some of the damage caused by free radicals, substances that damage DNA [8,9]. Studies show that vitamins B6 and C are essential for the natural synthesis of dopamine in the human body. On the other hand, large doses of vitamins B6 and C may reduce the risk of kidney stone formation in women. So, the simultaneous determination of these compounds is very important for pharmaceutical and biological investigation [10,11]. Several methods have been developed to determine vitamins B6 and C, including high-performance liquid chromatography [12], spectrophotometry [13], and flow injection [14,15]. However, these methods require not only advanced technical expertise but also time-consuming, expensive and often need the pretreatment step. Electrochemical detection is an attractive alternative approach to these technologies because of the inherent advantages of simplicity, ease of miniaturization, cost-effectiveness, dependability, high sensitivity, and relatively low cost [16-21]. So far, electrochemical sensors have found widespread use in a variety of disciplines, including pharmaceutical, food, and clinical analyses [22-29]. Currently, the advancement of screen-printing technology for the fabrication of screen-printed electrodes (SPEs) is attracting enormous attention due to the advantageous properties of SPEs compared to conventional electrodes, such as cost-effectiveness, disposability, simplicity, versatility, availability of materials and patterns, elimination of electrode maintenance, the requirement for low volumes of solution, and appropriateness for outside laboratory measurement [30-32]. Chemically modified electrodes are best suited for the electrochemical determination of pharmaceutical, environmental, or biological samples. Chemically modified electrodes reduce the over-potential required for either the oxidation or reduction of the electro-active compounds [33-39]. Also, modification of electrodes is a powerful strategy for overcoming such limitations of un-modified electrodes as low selectivity, poor sensitivity, low stability, and the blockage of the electron transfer [40-45]. Nanostructured metal oxides crystallizing in the spinel structure type have been investigated intensively over the years and present a permanent interest due to their wide technological applications such as magnetic and optical materials, semiconductors, pigments, catalysts, or material for biomedical applications [46-51]. Ferrites are a well-known class of complex oxides of considerable technological importance. On the other side, nano ZnFe2O4 as spinel ferrites is found to be one of the most interesting spinel systems because of its unique properties, photochemical stability, good visible-light response and favourable magnetism [52,53]. A characteristic of ZnFe2O4 is that it has two different metal cations, Zn and Fe, with O as an anion. The cations occupy two different positions in a spinel structure: tetrahedral (Zn) and octahedral (Fe) sites along the face- centered cubic lattice formed by O2− cations. The use of bimetallic oxides as electrode materials could enhance both electrical conductivity by two orders of magnitude and electrochemical activity versus materials prepared with unitary metal oxides [54,55]. In the present work, the preparation and application of a screen-printed graphite electrode, modified with zinc ferrite nano-particles (ZnFe2O4), for the determination of vitamin B6 in the presence of vitamin C is described. The electrochemical behavior of vitamin B6 at ZnFe2O4/SPGE was investigated. The results showed the superiority of ZnFe2O4/SPGE to the bare electrode in terms of better sensitivity. We have also evaluated the analytical performance of the ZnFe2O4/SPGE for the quantification of vitamin B6 in the presence of vitamin C in some real samples. ADMET & DMPK 11(2) (2023) 251-261 Determination of vitamin B6 in the presence of vitamin C doi: https://doi.org/10.5599/admet.1702 253 Experimental Chemicals and instrumentation All chemicals used were of analytical reagent grade purchased from Sigma-Aldrich and were used as received without any further purification. Double-distilled water was used throughout all experiments. Orthophosphoric acid was utilized to prepare the phosphate buffer solutions (PBSs), and sodium hydroxide was used to adjust the desired pH values (pH range between 2.0 and 9.0). Cyclic voltammetry (CV), linear sweep voltammetry (LSV), chronoamperometry, and differential pulse voltammetry (DPV) investigations were performed in an electroanalytical system Autolab PGSTAT302N, potentiostat/galvanostat connected to an electrode cell, the SPGE (DropSens; DRP-110: Spain), containing graphite counter electrode, a graphite working electrode, and a silver pseudo-reference electrode. The system was run on a PC using General Purpose Electrochemical System (GPES) software. Solution pH values were determined using a 713 pH meter combined with a glass electrode (Metrohm, Switzerland). Preparation of modified electrode ZnFe2O4 nano-particles were used to coat the bare screen printed graphite electrode. A stock solution of ZnFe2O4 nano-particles in 1 mL of the aqueous solution was prepared by distributing 1 mg of ZnFe2O4 nano- particles via ultra-sonication for 50 min, whereas 4 µL of aliquots of the ZnFe2O4 nano-particles suspension solution was cast on carbon working electrodes and evaporated at room temperature. Results and discussion Electrochemical behavior of vitamin B6 on the ZnFe2O4/SPGE According to our knowledge, the electrooxidation of vitamin B6 depends on the pH value of the solution (Scheme 1). So, the effect of pH was investigated using the DPV method. Results show that the oxidation peak current increased slowly from pH 2.0 to 7.0, and then the current conversely decreased when the pH value increased from 7.0 to 9.0. Consequently, pH 7.0 was chosen as the optimal experimental condition for other experiments. Scheme 1. The proposed mechanism for the oxidation of vitamin B6 at the ZnFe2O4/SPGE. To investigate the vitamin B6 behavior and the as-produced electrode response to vitamin B6, the performance of ZnFe2O4/SPGE was compared to that of unmodified SPGE. Figure 1 shows the CV curve obtained for ZnFe2O4/SPGE (curve a) and unmodified SPGE (curve b) in the presence of 200.0 μM vitamin B6- containing PBS at the scan rate of 50 mV/s. The results showed that the oxidation of vitamin B6 is very weak on the surface of the bare SPGE, but the presence of ZnFe2O4 nano-particles could enhance the peak current and decrease the oxidation potential (decreasing the overpotential). A substantial negative shift of the currents starting from oxidation potential for vitamin B6 and a dramatic increase of the current indicates the catalytic ability of ZnFe2O4/SPGE to vitamin B6 oxidation. The results showed that the use of ZnFe2O4 nano- particle improved the characteristics of vitamin B6 oxidation, which was partly due to excellent characteristics of ZnFe2O4 nano-particles such as excellent electrical conductivity and good chemical stability. https://doi.org/10.5599/admet.1702 P. M. Jahani et al. ADMET & DMPK 11(2) (2023) 251-261 254 Figure 1. Cyclic voltammograms of a) ZnFe2O4/SPGE and b) SPGE in the presence of 200.0 μM vitamin B6 at a pH 7.0 of 0.1 M PBS, respectively. Effect of scan rate The linear sweep voltammograms measurements were carried out to evaluate the association of peak current with scan rate at varied scan rates (10-400 mV/s) in the 100.0 μM vitamin B6-containing 0.1 M PBS (pH = 7.0) on the ZnFe2O4/SPGE (Figure 2). As shown in Figure 2, the peak currents of vitamin B6 grow with increasing scan rates and there are good linear relationships between the peak currents (Ip) and the square root of the scan rate (ν 1/2). The results also showed that the action is mass transfer of vitamin B6 controlled at diffusion process. To obtain further information on the rate-determining step, a Tafel plot was developed for the vitamin B6 at the surface of ZnFe2O4/SPGE using the data derived from the rising part of the current–voltage curve (Figure 3). The slope of the Tafel plot is equal to 2.3RT/n(1 − α)F, which comes up to 0.1803 V decade−1. We obtained the charge transfer coefficient (α) as 0.67. Chronoamperometric measurements The electrooxidation of vitamin B6 by a ZnFe2O4/SPGE was also studied by chronoamperometry (Figure 4). Chronoamperometric measurements of different concentrations of vitamin B6 at the ZnFe2O4/SPGE sensor were accomplished by setting the working electrode potential at 760 mV as the first step potential. Using chronoamperometric studies, we determined the diffusion coefficient, D, of vitamin B6 in a buffer solution. The experimental plots of Ip versus t-1/2 were employed with the best fits for different concentrations of vitamin B6 (Figure 4A). The slopes of the resulting straight lines were then plotted versus vitamin B6 concentrations (Figure 4B). Using these slopes and the Cottrell equation, we obtained D= 9.1×10-6 cm2 s-1. ADMET & DMPK 11(2) (2023) 251-261 Determination of vitamin B6 in the presence of vitamin C doi: https://doi.org/10.5599/admet.1702 255 Figure 2. Linear sweep voltammograms of vitamin B6 (100.0μM) at ZnFe2O4/SPGE at different scan rates of a) 10, b) 50, c) 100, d)200, e) 300, and f) 400 mV/s in 0.1 M PBS (pH 7.0). Insert: Plot of Ip versus ν 1/2 for the oxidation of vitamin B6 at ZnFe2O4/SPGE. Figure 3. Linear sweep voltammogram for ZnFe2O4/SPGE in the presence of 0.1 M PBS (Ph 7.0) with 100.0 µM of vitamin B6 at the scan rate of 10 mV/s; Points: outputs used in Tafel plot; Inset: Tafel plot of LSV. Figure 4. Chronoamperograms obtained at the ZnFe2O4/SPGE in the presence of a) 0.1, b) 0.3, c) 0.5, d) 1.0, e) 1.5, f) 2.0, g) 2.7, h) 3.2, and i) 3.5 μM vitamin B6 in the0.1 M buffer solution (pH 7.0). A) Plot of I versus t-1/2 for electrooxidation of vitamin B6 obtained from chronoamperograms a–i. B) Plot of slope from straight lines versus vitamin B6 level. Calibration plot and limit of detection Since DPV has a much higher current sensitivity and better resolution than CV and LSV, DPV was used for the determination of vitamin B6. Figure 5 shows the DPV curves of ZnFe2O4/SPGE in the PBS buffer with variable vitamin B6 levels (Step potential=0.01 V and pulse amplitude=0.025 V). It was found that the https://doi.org/10.5599/admet.1702 P. M. Jahani et al. ADMET & DMPK 11(2) (2023) 251-261 256 electrocatalytic peak currents of vitamin B6 oxidation at ZnFe2O4/SPGE surface linearly depended on vitamin B6 concentrations above the range of 0.8-585.0 µM (with a correlation coefficient of 0.9997), while determination limit was achieved to be 0.17 µM. Figure 5. DPV curves of ZnFe2O4/SPGE in the 0.1 M buffer solution (pH 7.0) containing different concentrations of vitamin B6. a-m corresponds to 0.8, 5.0, 10.0, 20.0, 40.0, 60.0, 80.0, 100.0, 200.0, 300.0, 400.0, 500.0, and 585.0 μM vitamin B6. Inset: Plots of electrocatalytic peak current as a function of vitamin B6 concentration. Determination of vitamin B6 in the presence of vitamin C The simultaneous determination of vitamin B6 and vitamin C is one of the most important applications of the proposed modified electrode. This study investigated a simultaneous change in the concentrations of vitamins B6 and C by recording the DPV curves. The result showed two well-defined oxidation peaks with a 530 mV separation of the peaks (Figure 6). Insets A and B in Figure 6 show the dependence of DPV peak currents on the concentration of vitamin B6 and vitamin C, respectively. The sensitivities towards vitamin B6 in the absence and presence of vitamin C were found to be 0.0501 µA/µM (in the absence of vitamin C) and 0.0503 µA/µM (in the presence of vitamin C). These results demonstrated that the ZnFe2O4/SPGE successfully detected vitamin B6 and vitamin C simultaneously, both sensitively and selectively. Stability of modified electrode For checking ZnFe2O4/SPGE sensor stability, we kept the recommended sensor within the pH equal to 7.0 in the PBS for two weeks to test ZnFe2O4/SPGE stability and, consequently, we recorded the DPV of the solution consisting of 50.0 μM vitamin B6 to be compared to the DPV observed prior to immersion. The oxidation peak of vitamin B6 did not change and, in comparison to earlier responses to the current, showed a less than 4.5 % reduction in signal, reflecting acceptable stability of ZnFe2O4/SPGE. ADMET & DMPK 11(2) (2023) 251-261 Determination of vitamin B6 in the presence of vitamin C doi: https://doi.org/10.5599/admet.1702 257 Figure 6. differential pulse voltammograms of ZnFe2O4/SPGE in 0.1 M PBS (pH 7.0) containing different concentrations of vitamin C and vitamin B6 mixed solutions of: a)10.0+15.0 , b) 50.0+50.0, c) 125.0+100.0, d) 300.0+150.0, e) 400.0+200.0, f) 600.0+300.0, g) 800.0+400.0,and h) 1000.0+500.0 µM vitamin C and vitamin B6, respectively. Insets: (A) plot of the peak currents as a function of vitamin C concentration and (B) plot of the peak currents as a function of vitamin B6 concentration. Conclusion A sensor for voltammetric determination of traces of vitamin B6 in real samples, based on the Zn-ferrite modified screen printed graphite electrode, was developed. The sensor exhibited a good linear response over the concentration range 0.8-585.0 µM with a detection limit of 0.17 µM for vitamin B6. Also, the modified electrode successfully resolves the overlapped voltammetric peaks of vitamin B6 and vitamin C by approximately 530 mV so that the modified electrode displays high selectivity in the DPV measurement of vitamin B6 and vitamin C of in their mixture solutions. As well as, the proposed method could be applied to the determination of vitamin B6 and vitamin C in real samples. Conflict of interest: The authors declare no conflict of interest. References [1] S. Abdulmlik, P. Saifullah, M.A. Al-a’adhami. Evaluation of vitamin B12 and methylmalonic acid levels as markers with neuropathy in patients of type 2 diabetes mellitus. Eurasian Chemical Communications 4(10) (2022) 956-965. https://doi.org/10.22034/ecc.2022.339922.1452 [2] B. Brunetti. Recent advances in electroanalysis of vitamins. Electroanalysis 28(9) (2016) 1930-1942. https://doi.org/10.1002/elan.201600097 [3] B. Habibi, H. Phezhhan, M.H. Pournaghi-Azar. Voltammetric determination of vitamin B6 (pyridoxine) using multi wall carbon nanotube modified carbon-ceramic electrode. journal of the Iranian Chemical Society 7 (2010) S103-S112. https://doi.org/10.1007/BF03246189 [4] S. Rison, A.T. Mathew, L. George, T. Maiyalagan, G. Hegde, A. Varghese, Pt nanospheres decorated graphene-β-CD modified pencil graphite electrode for the electrochemical determination of vitamin B6. Topics in Catalysis (2022) 1-11. https://doi.org/10.1007/s11244-021-01559-1 https://doi.org/10.5599/admet.1702 https://doi.org/10.22034/ecc.2022.339922.1452 https://doi.org/10.1002/elan.201600097 https://doi.org/10.1007/BF03246189 https://doi.org/10.1007/s11244-021-01559-1 P. M. Jahani et al. ADMET & DMPK 11(2) (2023) 251-261 258 [5] S.M. Cottica, J. Nozaki, H.S. Nakatani, C.C. Oliveira, N.E.D. Souza, J.V. Visentainer. Voltammetric determination of pyridoxine (vitamin B6) in drugs using a glassy carbon electrode modified with chromium (III) hexacyanoferrate (II). Journal of the Brazilian Chemical Society 20 (2009) 496-501. https://doi.org/10.1590/S0103-50532009000300014 [6] S.J. Devaki, R.L. Raveendran. Vitamin C: sources, functions, sensing and analysis. In Vitamin C. IntechOpen (2017). https://doi.org/10.5772/intechopen.70162 [7] J.M. Zen, D.M. Tsai, H.H. Yang, Direct determination of vitamin C in fruit juices using a polyviologen‐ modified electrode. Electroanalysis: An International Journal Devoted to Fundamental and Practical Aspects of Electroanalysis 14(22) (2002) 1597-1600. https://doi.org/10.1002/1521-4109(200211)14:22 %3C1597::AID-ELAN1597%3E3.0.CO;2-U. [8] I. Škugor Rončević, D. Skroza, I. Vrca, A.M. Kondža, N. Vladislavić. Development and optimization of electrochemical method for determination of vitamin C. Chemosensors 10(7) (2022) 283. https://doi.org/ 10.3390/chemosensors10070283 [9] F. Shayanfar, H. Sarhadi. Determination of vitamin C at modified screen printed electrode: Application for sensing of vitamin C in real samples. Surface Engineering and Applied Electrochemistry 57 (2021) 487- 494. https://doi.org/10.3103/S1068375521040141 [10] A. Baghizadeh, H. Karimi-Maleh, Z. Khoshnama, A. Hassankhani, M. Abbasghorbani. A voltammetric sensor for simultaneous determination of vitamin C and vitamin B 6 in food samples using ZrO 2 nano- particle/ionic liquids carbon paste electrode. Food Analytical Methods 8 (2015) 549-557. https://doi.org/ 10.1007/s12161-014-9926-3 [11] H. Bakhsh, I.M. Palabiyik, R.K. Oad, N. Qambrani, J.A. Buledi, A.R. Solangi, S.T.H. Sherazi. SnO2 nanostructure based electroanalytical approach for simultaneous monitoring of vitamin C and vitamin B6 in pharmaceuticals. Journal of Electroanalytical Chemistry 910 (2022) 116181. https://doi.org/10.10 16/j.jelechem.2022.116181 [12] T.K. Patle, K. Shrivas, A. Patle, S. Patel, N. Harmukh, A. Kumar. Simultaneous determination of B1, B3, B6 and C vitamins in green leafy vegetables using reverse phase-high performance liquid chromatography. Microchemical Journal 176 (2022) 107249. https://doi.org/10.1016/j.microc.2022.107249 [13] Y.B. Monakhova, S.P. Mushtakova, S.S. Kolesnikova. Determination of vitamins in mixtures of various composition by spectrophotometry with self-modeling curve resolution. Journal of Analytical Chemistry 65 (2010) 588-595. https://doi.org/10.1134/S1061934810060079 [14] L.G. Shaidarova, L.N. Davletshina, G.K. Budnikov. Flow-injection determination of water-soluble vitamins B 1, B 2, and B 6 from the electrocatalytic response of a graphite electrode modified with a ruthenium (III) hexacyanoruthenate (II) film. Journal of Analytical Chemistry 61 (2006) 502-509. https://doi.org/ 10.1134/S1061934806050133 [15] M. Kolar, D. Dobcnik, N. Radić. Potentiometric flow-injection determination of vitamin C and glutathione with a chemically prepared tubular silver electrode. Die Pharmazie 55(12) (2000) 913-916. [16] H. Karimi-Maleh, F. Karimi, Y. Orooji, G. Mansouri, A. Razmjou, A. Aygun, F. Sen. A new nickel-based co- crystal complex electrocatalyst amplified by NiO dope Pt nanostructure hybrid; a highly sensitive approach for determination of cysteamine in the presence of serotonin. Scientific Reports 10(1) (2020) 1-13. https://doi.org/10.1038/s41598-020-68663-2 [17] M. Bijad, A. Hojjati-Najafabadi, H. Asari-Bami, S. Habibzadeh, I. Amini, F. Fazeli. An overview of modified sensors with focus on electrochemical sensing of sulfite in food samples. Eurasian Chemical Communications 3(2) (2021) 116-138. https://doi.org/10.22034/ecc.2021.268819.1122 [18] H. Beitollahi, S.Z. Mohammadi, M. Safaei, S. Tajik. Applications of electrochemical sensors and biosensors based on modified screen-printed electrodes: a review. Analytical Methods 12 (2020) 1547- 1560. https://doi.org/10.1039/C9AY02598G [19] S. Saghiri, M. Ebrahimi, M. Bozorgmehr. Electrochemical Amplified Sensor with Mgo Nano- particle and Ionic Liquid: A Powerful Strategy for Methyldopa Analysis. Chemical Methodologies 5(3) (2021) 234-239. https://doi.org/10.22034/chemm.2021.128530 https://doi.org/10.1590/S0103-50532009000300014 https://doi.org/10.5772/intechopen.70162 https://doi.org/10.1002/1521-4109(200211)14:22%3C1597::AID-ELAN1597%3E3.0.CO;2-U https://doi.org/10.1002/1521-4109(200211)14:22%3C1597::AID-ELAN1597%3E3.0.CO;2-U https://doi.org/10.3390/chemosensors10070283 https://doi.org/10.3390/chemosensors10070283 https://doi.org/10.3103/S1068375521040141 https://doi.org/10.1007/s12161-014-9926-3 https://doi.org/10.1007/s12161-014-9926-3 https://doi.org/10.1016/j.jelechem.2022.116181 https://doi.org/10.1016/j.jelechem.2022.116181 https://doi.org/10.1016/j.microc.2022.107249 https://doi.org/10.1134/S1061934810060079 https://doi.org/10.1134/S1061934806050133 https://doi.org/10.1134/S1061934806050133 https://doi.org/10.1038/s41598-020-68663-2 https://doi.org/10.22034/ecc.2021.268819.1122 https://doi.org/10.1039/C9AY02598G https://doi.org/10.22034/chemm.2021.128530 ADMET & DMPK 11(2) (2023) 251-261 Determination of vitamin B6 in the presence of vitamin C doi: https://doi.org/10.5599/admet.1702 259 [20] A. Shamsi, F. Ahour. Electrochemical Sensing of Thioridazine in Human Serum Samples Using Modified Glassy Carbon Electrode. Advanced Journal of Chemistry-Section A 4(1) (2021) 22-31. https://doi.org/ 10.22034/ajca.2020.252025.1215 [21] J. Mohanraj, D. Durgalakshmi, R.A. Rakkesh, S. Balakumar, S. Rajendran, H. Karimi-Maleh. Facile synthesis of paper based graphene electrodes for point of care devices: A double stranded DNA (dsDNA) biosensor. Journal of Colloid and Interface Science 566 (2020) 463-472. https://doi.org/- 10.1016/j.jcis.2020.01.089 [22] M. Mazloum-Ardakani, H. Beitollahi, B. Ganjipour, H. Naeimi. Novel carbon nanotube paste electrode for simultaneous determination of norepinephrine, uric acid and d-penicillamine. International Journal of Electrochemical Science 5 (2010) 531-546. [23] A. Lohrasbi-Nejad. Electrochemical strategies for detection of diazinon. Journal of Electrochemical Science and Engineering 12(6) (2022) 1041-1059. https://doi.org/10.5599/jese.1379 [24] M.S. Sengar, S. Saxena, S.P. Satsangee, R. Jain. Silver Nano-particles Decorated Functionalized Multiwalled Carbon Nanotubes Modified Screen Printed Sensor for Voltammetric Determination of Butorphanol. Journal of Applied Organometallic Chemistry 1(2) (2021) 95-108. https://doi.org/10.22034/jaoc.2021.289344.1023 [25] Y. F. Mustafa, G. Chehardoli, S. Habibzadeh, Z. Arzehgar. Electrochemical detection of sulfite in food samples. Journal of Electrochemical Science and Engineering 12(6) (2022) 1061-1079. https://doi.org/ 10.5599/jese.1555 [26] M.M. Ardakani, Z. Taleat, H. Beitollahi, M. Salavati-Niasari, B.B.F. Mirjalili, N. Taghavinia. Electrocatalytic oxidation and nanomolar determination of guanine at the surface of a molybdenum (VI) complex–TiO2 nano-particle modified carbon paste electrode. Journal of Electroanalytical Chemistry 624 (2008) 73-78. https://doi.org/10.1016/j.jelechem.2008.07.027 [27] M. Miraki, H. Karimi-Maleh, M. A. Taher, S. Cheraghi, F. Karimi, S. Agarwal, V. K. Gupta. Voltammetric amplified platform based on ionic liquid/NiO nanocomposite for determination of benserazide and levodopa. Journal of Molecular Liquids 278 (2019) 672-676. https://doi.org/10.1016/j.molliq.20 19.01.081 [28] A. Hosseini Fakhrabad, R. Sanavi Khoshnood, M.R. Abedi, M. Ebrahimi. Fabrication a composite carbon paste electrodes (CPEs) modified with multi-wall carbon nano-tubes (MWCNTs/N, N-Bis (salicyliden)-1,3-propandiamine) for determination of lanthanum (III). Eurasian Chemical Communications 3(9) (2021) 627-634. https://doi.org/10.22034/ecc.2021.288271.1182 [29] S. Mohammadi, H. Beitollahi, A. Mohadesi. Electrochemical behaviour of a modified carbon nanotube paste electrode and its application for simultaneous determination of epinephrine, uric acid and folic acid. Sensor Letters 11(2) (2013) 388-394. https://doi.org/10.1166/sl.2013.2723 [30] H. Beitollahi, N. Arbabi. NanolayeredTi3C2 Modified Screen Printed Electrode as High‐Performance Electrode for Electrochemical Detection of Tyrosine. Chemical Methodologies 6(4) (2022) 293-300. https://doi.org/10.22034/chemm.2022.328263.1436 [31] O.M. Istrate, L. Rotariu, C. Bala. Amperometric L-Lactate biosensor based upon a gold nano- particles/reduced graphene oxide/polyallylamine hydrochloride modified screen-printed graphite electrode. Chemosensors 9(4) (2021) 74. https://doi.org/10.3390/chemosensors9040074 [32] E.P. Medyantseva, D.V. Brusnitsyn, R.M. Varlamova, M.A. Baibatarova, G.K. Budnikov, A.N. Fattakhova. Determination of antidepressants using monoamine oxidase amperometric biosensors based on screen- printed graphite electrodes modified with multi-walled carbon nanotubes. Pharmaceutical Chemistry Journal 48 (2014) 478-482. https://doi.org/10.1007/s11094-014-1135-2 [33] Z. Mehdizadeh, S. Shahidi, A. Ghorbani-HasanSaraei, M. Limooei, M. Bijad. Monitoring of Amaranth in Drinking Samples using Voltammetric Amplified Electroanalytical Sensor. Chemical Methodologies 6(3) (2022) 246-252. https://doi.org/10.22034/chemm.2022.324073.1423 [34] H. Karimi-Maleh, M. Sheikhshoaie, I. Sheikhshoaie, M. Ranjbar, J. Alizadeh, N.W. Maxakato, A. Abbaspourrad. A novel electrochemical epinine sensor using amplified CuO nano-particles and an-hexyl- https://doi.org/10.5599/admet.1702 https://doi.org/10.22034/ajca.2020.252025.1215 https://doi.org/10.22034/ajca.2020.252025.1215 https://doi.org/10.1016/j.jcis.2020.01.089 https://doi.org/10.1016/j.jcis.2020.01.089 https://doi.org/10.5599/jese.1379 https://doi.org/10.22034/jaoc.2021.289344.1023 https://doi.org/10.5599/jese.1555 https://doi.org/10.5599/jese.1555 https://doi.org/10.1016/j.jelechem.2008.07.027 https://doi.org/10.1016/j.molliq.20‌19.01.081 https://doi.org/10.1016/j.molliq.20‌19.01.081 https://doi.org/10.22034/ecc.2021.288271.1182 https://doi.org/10.1166/sl.2013.2723 https://doi.org/10.22034/chemm.2022.328263.1436 https://doi.org/10.3390/chemosensors9040074 https://doi.org/10.1007/s11094-014-1135-2 https://doi.org/10.22034/chemm.2022.324073.1423 P. M. Jahani et al. ADMET & DMPK 11(2) (2023) 251-261 260 3-methylimidazolium hexafluorophosphate electrode. New Journal of Chemistry 43(5) (2019) 2362- 2367. https://doi.org/10.1039/C8NJ05581E [35] R.M. Mohabis, F. Fazeli, I. Amini, V. Azizkhani. An overview of recent advances in the detection of ascorbic acid by electrochemical techniques. Journal of Electrochemical Science and Engineering 12(6) (2022) 1081-1098. https://doi.org/10.5599/jese.1561 [36] S. Azimi, M. Amiri, H. Imanzadeh, A. Bezaatpour. Fe3O4@SiO2-NH2/CoSB Modified Carbon Paste Electrode for Simultaneous Detection of Acetaminophen and Chlorpheniramine. Advanced Journal of Chemistry-Section A 4(2) (2021) 152-164. https://doi.org/10.22034/ajca.2021.275901.1246 [37] S.A. Alavi-Tabari, M.A. Khalilzadeh, H. Karimi-Maleh. Simultaneous determination of doxorubicin and dasatinib as two breast anticancer drugs uses an amplified sensor with ionic liquid and ZnO nano- particle. Journal of Electroanalytical Chemistry 811 (2018) 84-88. https://doi.org/10.1016/j.jelechem.20 18.01.034 [38] M. Mirzaei, O. Gulseren, M. Rafienia, A. Zare. Nanocarbon-assisted biosensor for diagnosis of exhaled biomarkers of lung cancer: DFT approach. Eurasian Chemical Communications 3(3) (2021) 154- 161. http://dx.doi.org/10.22034/ecc.2021.269256.1126, [39] P.M. Jahani. Flower-like MoS2 screen-printed electrode based sensor for the sensitive detection of sunset yellow FCF in food samples. Journal of Electrochemical Science and Engineering 12(6) (2022) 1099-1109. https://doi.org/10.5599/jese.1413 [40] H. Karimi-Maleh, A.F. Shojaei, K. Tabatabaeian, F. Karimi, S. Shakeri, R. Moradi. Simultaneous determination of 6-mercaptopruine, 6-thioguanine and dasatinib as three important anticancer drugs using nanostructure voltammetric sensor employing Pt/MWCNTs and 1-butyl-3-methylimidazolium hexafluoro phosphate. Biosensors and Bioelectronics 86 (2016) 879-884. https://doi.org/10.1016/j.bi os.2016.07.086 [41] M. Vardini, N. Abbasi, A. Kaviani, M. Ahmadi, E. Karimi. Graphite Electrode Potentiometric Sensor Modified by Surface Imprinted Silica Gel to Measure Valproic Acid. Chemical Methodologies 6(5) (2022) 398-408. https://doi.org/10.22034/chemm.2022.328620.1437 [42] S. Ariavand, M. Ebrahimi, E. Foladi. Design and Construction of a Novel and an Efficient Potentiometric Sensor for Determination of Sodium Ion in Urban Water Samples. Chemical Methodologies 6(11) (2022) 886-904. https://doi.org/10.22034/chemm.2022.348712.1567 [43] J.B. Raoof, R. Ojani, H. Beitollahi, R. Hosseinzadeh. Electrocatalytic oxidation and highly selective voltammetric determination of L-cysteine at the surface of a 1-[4-(ferrocenyl ethynyl) phenyl]-1- ethanone modified carbon paste electrode. Analytical Sciences 22(9) (2006) 1213-1220. https://doi.org/ 10.2116/analsci.22.1213 [44] T. Eren, N. Atar, M. L. Yola, H. Karimi-Maleh. A sensitive molecularly imprinted polymer based quartz crystal microbalance nanosensor for selective determination of lovastatin in red yeast rice. Food Chemistry 185 (2015) 430-436. https://doi.org/10.1016/j.foodchem.2015.03.153 [45] S. Saghiri, M. Ebrahimi, M.R. Bozorgmehr. NiO nanoparticle/1-hexyl-3-methylimidazolium hexafluorophosphate composite for amplification of epinephrine electrochemical sensor. Asian Journal of Nanosciences and Materials 4(1) (2021) 46-52. https://doi.org/10.26655/AJNANOMAT.2021.1.4 [46] Y. Dessie, S. Tadesse. A Review on Advancements of Nanocomposites as Efficient Anode Modifier Catalyst for Microbial Fuel Cell Performance Improvement. Journal of Chemical Reviews 3(4) (2021) 320- 344. https://doi.org/10.22034/jcr.2021.314327.1128 [47] Z.R. Al-Bahadili, A.A.S. Al-Hamdani, L.A. Al-Zubaidi, F.A. Rashid, S.M. Ibrahim. An Evaluation of the Activity of Prepared Zinc Nano-Particles with Extract Alfalfa Plant in the Treatments of Peptidase and Ions in Water. Chemical Methodologies 6(7) (2022) 522-533. https://doi.org/10.22034/chemm.2022.- 336588.1470 [48] E. Ezzatzadeh. Chemoselective oxidation of sulfides to sulfoxides using a novel Zn-DABCO functionalized Fe3O4 MNPs as highly effective nanomagnetic catalyst. Asian Journal of Nanosciences and Materials 4(2) (2021) 125-136. https://doi.org/10.26655/AJNANOMAT.2021.2.3 https://doi.org/10.1039/C8NJ05581E https://doi.org/10.5599/jese.1561 https://doi.org/10.22034/ajca.2021.275901.1246 https://doi.org/10.1016/j.jele‌chem.2018.01.034 https://doi.org/10.1016/j.jele‌chem.2018.01.034 http://dx.doi.org/10.22034/ecc.2021.269256.1126 https://doi.org/10.5599/jese.1413 https://doi.org/10.1016/j.bios.2016.07.086 https://doi.org/10.1016/j.bios.2016.07.086 https://doi.org/10.22034/chemm.2022.328620.1437 https://doi.org/10.22034/chemm.2022.348712.1567 https://doi.org/10.2116/analsci.22.1213 https://doi.org/10.2116/analsci.22.1213 https://doi.org/10.1016/j.foodchem.2015.03.153 https://doi.org/10.26655/AJNANOMAT.2021.1.4 http://www.jchemrev.com/article_140627.html http://www.jchemrev.com/article_140627.html https://doi.org/10.22034/jcr.2021.314327.1128 https://doi.org/10.22034/chemm.2022.336588.1470 https://doi.org/10.22034/chemm.2022.336588.1470 https://doi.org/10.26655/AJNANOMAT.2021.2.3 ADMET & DMPK 11(2) (2023) 251-261 Determination of vitamin B6 in the presence of vitamin C doi: https://doi.org/10.5599/admet.1702 261 [49] H. Karimi-Maleh, C. Karaman, O. Karaman, F. Karimi, Y. Vasseghian, L. Fu, A. Mirabi. Nanochemistry approach for the fabrication of Fe and N co-decorated biomass-derived activated carbon frameworks: a promising oxygen reduction reaction electrocatalyst in neutral media. Journal of Nanostructure in Chemistry (2022)1-11. https://doi.org/10.1007/s40097-022-00492-3 [50] F. Zare Kazemabadi, A. Heydarinasab, A. Akbarzadehkhiyavi, M. Ardjmand. Development, Optimization and In vitro Evaluation of Etoposide loaded Lipid Polymer Hybrid Nano-particles for controlled Drug Delivery on Lung Cancer. Chemical Methodologies 5(2) (2021) 135-152. https://doi.org/10.22034/chemm.2021.121495 [51] Y.Y. Muhi-Alden, K.A. Saleh. Removing methylene blue dye from industrial wastewater using polyacrylonitrile/iron oxide nanocomposite. Eurasian Chemical Communications 3(10) (2021) 755-762. http://dx.doi.org/10.22034/ecc.2021.300767.1225 [52] G. Osamong, P.K. Kamweru, J.M. Gichumbi, F.G. Ndiritu. Surface potential, fermi level and band gap energy of copper doped magnesium nickel ferrite nano-particles. Asian Journal of Nanosciences and Materials 4(1) (2021) 1-14. https://doi.org/10.26655/AJNANOMAT.2021.1.1 [53] M. Ognjanović, D.M. Stanković, Y. Ming, H. Zhang, B. Jančar, B. Dojčinović, B. Antić. Bifunctional (Zn, Fe) 3O4 nano-particles: Tuning their efficiency for potential application in reagentless glucose biosensors and magnetic hyperthermia. Journal of Alloys and Compounds 777 (2019) 454-462. https://doi.org/10.1016/j.jallcom.2018.10.369 [54] D.M. Stanković, S. Škrivanj, N. Savić, A. S. Nikolić, P. Vulić, D.D. Manojlović. Application of Novel Zn‐ Ferrite Modified Glassy Carbon Paste Electrode as a Sensor for Determination of Cd (II) in Waste Water. Electroanalysis 26(7) (2014) 1536-1543. https://doi.org/10.1002/elan.201400095 [55] A.V. Shinde, S.J. Patil, S.K. Hwang, G.S.R. Raju, Y.S. Huh, Y.K. Han, N.R. Chodankar. Surface modified zinc ferrite as a carbon-alternative negative electrode for high-energy hybrid supercapacitor. Ceramics International 47(11) (2021) 16333-16341. https://doi.org/10.1016/j.ceramint.2021.02.213 ©2023 by the authors; licensee IAPC, Zagreb, Croatia. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/) https://doi.org/10.5599/admet.1702 https://doi.org/10.1007/s40097-022-00492-3 https://doi.org/10.22034/chemm.2021.121495 http://dx.doi.org/10.22034/ecc.2021.300767.1225 https://doi.org/10.26655/AJNANOMAT.2021.1.1 https://doi.org/10.1016/j.jallcom.2018.10.369 https://doi.org/10.1002/elan.201400095 https://doi.org/10.1016/j.ceramint.2021.02.213 http://creativecommons.org/licenses/by/3.0/