Recent advances in electrochemical sensors and biosensors for monitoring drugs and metabolites in pharmaceutical and biological samples doi: https://doi.org/10.5599/admet.1709 151 ADMET & DMPK 11(2) (2023) 151-173; doi: https://doi.org/10.5599/admet.1709 Open Access : ISSN : 1848-7718 http://www.pub.iapchem.org/ojs/index.php/admet/index Review Recent advances in electrochemical sensors and biosensors for monitoring drugs and metabolites in pharmaceutical and biological samples Charaf Laghlimi*,1, Abdelaziz Moutcine2, Abdelilah Chtaini2, Jalal Isaad1, Adil Soufi1, Younes Ziat3, Hassan Amhamdi4 and Hamza Belkhanchi3 1ERCI2A, FSTH, Abdelmalek Essaadi University, Tetouan, Morocco 2Molecular Electrochemistry and Inorganic Materials Team, Faculty of Science and Technology of Beni Mellal, Sultan Moulay Slimane University, Morocco 3Laboratory of Engineering and Applied Technologies, Higher School of Technology, Sultan Moulay Slimane University, Beni Mellal, Morocco 4Applied Chemistry Team, FSTH, Abdelmalek Essaadi University, Tetouan, Morocco *Corresponding Author: E-mail: charaf.cac.fbs@gmail.com;c.laghlimi@uae.ac.ma; Tel.: +0706182632 Received: February 15, 2023; Revised: April 7, 2023; Published: May 1, 2023 Abstract Various applications of electrochemical sensors and biosensors have been reported in many fields. These include pharmaceuticals, drug detection, cancer detection, and analysis of toxic elements in tap water. Electrochemical sensors are characterised by their low cost, ease of manufacture, rapid analysis, small size and ability to detect multiple elements simultaneously. They also allow the reaction mechanisms of analytes, such as drugs, to be taken into account, giving a first indication of their fate in the body or their pharmaceutical preparation. Several materials are used in the construction of sensors, such as graphene, fullerene, carbon nanotubes, carbon graphite, glassy carbon, carbon clay, graphene oxide, reduced graphene oxide, and metals. This review covers the most recent progress in electrochemical sensors used to analyze drugs and metabolites in pharmaceutical and biological samples. We have highlighted carbon paste electrodes (CPE), glassy carbon electrodes (GCE), screen-printed carbon electrodes (SPCE) and reduced graphene oxide electrodes (rGOE). The sensitivity and analysis speed of electrochemical sensors can be improved by modifying them with conductive materials. Different materials used for modification have been reported and demonstrated, such as molecularly imprinted polymers, multiwalled carbon nanotubes, fullerene (C60), iron(III) nanoparticles (Fe3O4NP), and CuO micro-fragments (CuO MF). Manufacturing strategies and the detection limit of each sensor have been reported. ©2023 by the authors. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). Keywords Pharmaceuticals and drugs; screen printed carbon electrodes; glassy carbon electrodes, carbon nanotubes Introduction Pharmacokinetics aims to determine a drug's pathway from administration to excretion [1]. For clinical diagnosis, there is a need for information on how medicines work in the human body and the dose that should be administered [2]. It is, therefore, necessary to quantify drugs in physiological fluids such as serum, urine, sweat and saliva, as well as in pharmaceutical tablets. Several sensitive and accurate methods have been used https://doi.org/10.5599/admet.1709 https://doi.org/10.5599/admet.1709 http://www.pub.iapchem.org/ojs/index.php/admet/index mailto:charaf.cac.fbs@gmail.com mailto:c.laghlimi@uae.ac.ma http://creativecommons.org/licenses/by/4.0/ C. Laghlimi et al. ADMET & DMPK 11(2) (2023) 151-173 152 to quantify and detect different types of drugs, such as spectrophotometry [3,4], fluorimetry [3], RP-HPLC [4], HPLC-diode array detector, TLC-densitometric methods [5] and capillary zone electrophoresis [6]. However, these techniques are very expensive and require the use of expensive solvents, specialised technicians, long sample preparation times and expensive instrumentation, all of which increase the cost and duration of the analysis [2]. For this reason, the pharmacokinetic community strives to provide alternative methods of drug surveillance that are easy, inexpensive, sensitive and precise [7]. In the last decades, electrochemical sensors have become an interesting alternative technique due to their high capacity to analyse drugs in different physio- logical fluids [8-10]. Graphene has considerable sensitivity and selectivity and often has a large potential window to accommodate the redox process involved. It also has a significant electron transfer process due to its planar sites and the presence of σ and π bonds, making it an attractive material for sensor manufacture [11,12]. Modification of the carbon paste electrode offers high sensitivity, low cost and rapid analysis without any pre-treatment [13-21]. The modification is an effective way to reduce the overpotential and increase the sensitivity of the sensors [22]. Various electrodes based on carbon as a conductor have been used for the electroanalysis of drugs [23,25]. In this review, we report on a new advance in carbon graphite-based electrochemical sensors used to analyse different types of drugs in pharmaceutical tablets and a variety of physiological fluids such as serum, blood, sweat and urine. The different modifiers used to improve sensor performance and increase the electroactive surface area are studied. Sensitivity, detection limit and method of preparation have been highlighted to provide comprehensive information on all these techniques and their use in drug analysis. Electrodes based on modified carbon paste for detecting various drugs and metabolites Carbon paste electrodes (CPE) have a very large electroactive surface area, which can be renewed for a variety of applications. In addition, it has low ohmic resistance and high stability, reproducibility and lifetime, allowing it to analyse various drugs, revealing the oxidation and reduction processes involved due to its wide potential range. The sensitivity and speed of analysis of CPE can be significantly improved by modifying them with conductive materials. Different types of modifiers have been used. The modified CPE has been used to examine various drugs in various samples, including human urine, pharmaceutical formulations, plasma and serum. The carbon paste electrode modified with poly(EBT) [26] exhibits very high electrocatalytic and semicon- ducting for detecting methdilazine hydrochloride (MDH), an antihistamine drug, in Dilosyn syrup and human urine using the SWV method (Figure 1). The MDH oxidation peak is observed at 0.675V with higher intensity compared to unmodified CPE. The surface area of poly-EBT/CPE is calculated to be 0.097 cm2, which is 2.30 times higher than that of CPE. The developed sensor shows good accuracy in both media (average recovery is 98.14 % in Dilosyn syrup and 97.4 % in human urine). The developed sensor has a low LOD of the order of 10-8 mol l-1 in the range of 0.1-50 μmol l-1 compared to other methods such as visible spectrophotometry (3.23 μmol l-1) [27], ultra-high performance reversed phase chromatography (0.254 μmol l-1 ) [28] and spectro- photometric method (1.62 μmol l-1 ) [29]. The researchers [30] reported that CPE/nanozeolite type X outperformed CPE/nanozeolite type A with a LOD of 0.2 μmol l-1 for paracetamol and 8 mmol l-1 for epinine. A recent study described the analysis of the antifungal agent ketoconazole (KTC) in pharmaceutical and urine samples using Ce-BTC MOF/IL/CPE (cf. Table 1) [31]. The modifiers are synthesised according to the method of Liu [32]. The electrochemical behaviour is studied by chronoamperometry (CA), differential pulse voltammetry (DPV), cyclic voltammetry (CV) and linear sweep voltammetry (LSV). The LOD of Ce-BTC MOF/IL/CPE is 0.04 μmol l-1 in the range of 0.1-110.0 μmol l-1 . The sensitivity is found to be 0.1342 μA μmol-1 l. ADMET & DMPK 11(2) (2023) 151-173 Electrochemical sensors and biosensors for monitoring drugs and metabolites doi: https://doi.org/10.5599/admet.1709 153 Figure 1. Schematic of A) EBT/CPE preparation, B) polymerisation steps of modified CPE, C) proposed mechanism of eriochrome black T (EBT) polymerisation and D) electrochemical detection of MDH by EBT/CPE. Carried out at the base of the reference [26] with an order License ID of 1346497-1 J. Zoubir et al. [33] used silver nanoparticles to modify carbon graphite by electrodeposition. The fabri- cated sensor was used to detect metronidazole in milk and tap water with a detection limit of 0.206 μmol l-1 in the range 1 -1000 μmol l-1 . O. Vajdle et al. [34] used the drop coating method to modify CPE with gold nanoparticles. AuNPs/CPE were used to analyse four macrolide antibiotics by SWV. AuNPs/CPEs are used for azithromycin (AZI) detection in Hemomycin® by the SWV method. The oxidation peak of the AZI is identified at 0.77 V on the AuNPs/CPE, while the roxithromycin (ROX) peak is found at 0.65 V by CPE in the Runac®. Both electrodes were found to be valid by comparison with HPLC-DAD measurements. On the other hand, nitric acid and sulphuric acid are used to increase -OH and -COOH groups on the surface of MWCNTs, which increases the surface area of the material [35]. In addition, the numerous carboxyl groups inhibit π-π interactions, which leads to a decrease in the adsorbency of MWCNTs [36]. Ofloxacin (OFX) adsorbs on MWCNT by binding its fluorine group to the -OH group of the CNT [37-40]. Flake graphite (FG) is used to increase the conductivity of MWCNTs weakened by the adsorption of compounds owing to its high degree of crystallisation. For this reason, M. Elfiky et al. [41] prepared an electrode by mixing graphite powder with FG and MWCNTs. The sensor has a larger electroactive area of 2.08 cm2 compared to the unmodified CPEs, with only 1.14 cm2. This finding is due to cracks on the irregular compact layers of the electrode. The electrode presented by the [10%FG/5%MW] CPE shows excellent performance in the analysis of ofloxacin in a commercial formulation (Ofloxacin® tablets) and in human urine samples with a LOD of 0.18 nmol l-1. OFX peak is observed at 0.85V by SW-AdAS The analysis of AZI, an alkaline chemical, by HPLC presents a difficulty due to its adsorption on Si-OH chromatographic materials [42]. Therefore, electrochemiluminescence detection is considered an alternative method for the detection of this antibiotic due to its sensitivity and high productivity [43,44]. Electrochemiluminescence (ECL) is based on the redox process involved in the formation of excited states that are able to emit light. The concept of molecularly imprinted polymers (MIP) is based on imprinting a molecular cavity on the surface of the polymer using a template molecule, which is then removed while the https://doi.org/10.5599/admet.1709 https://www.sciencedirect.com/topics/chemistry/azithromycin https://www.sciencedirect.com/topics/chemistry/roxithromycin C. Laghlimi et al. ADMET & DMPK 11(2) (2023) 151-173 154 polymer is still imprinted. This MIP can then be used to capture and detect a target molecule similar to the template molecule and specifically binds to a functional group in the cavity in the same way that an antibody binds to its antigen. There are many ways to make MIPs, such as soft lithography applied to giant molecules. L. Hu et al. [45] successfully analysed azithromycin (AZI) in urine and serum samples using MIP/CP ECL sensor, with MIP used as a recognisor. The oxidation peak of the amino groups of AZI [31] is observed at 0.8 V with LOD of 23 pmol l-1 in the range of 0.10 - 400 nmol l-1. The analytical results show the MIP/CP ECL sensor and HPLC agreement. Clay has attractive characteristics, like high specific surface area and cation exchange capacity [46,47]. Bakary Tigana Djonse Justin et al. [48] recently fabricated a titanium dioxide modified carbon clay paste electrode (CPEA/TiO2/UV) that allows the analysis of ascorbic acid in pharmaceutical tablets using CV.The sensitivity of the electrode is enhanced by photoactivation of TiO2 by light irradiation (with a 100 W lamp), which produces electron (e)/hole (h+) pairs upon absorption of appropriate light energy [49]. The detection limit is 0.732 μmol l-1in the range of 0.15-0.850 μmol l-1 . V. Vinoth et al. [50] modified a graphite carbon electrode with CuO microflowers (MFs) by solubilising them in Nafion to facilitate the modification. This electrode was named CuO MFs/Nafion/GC. The CuO MFs/Nafion/GC exhibited perfect electrocatalytic properties, selectivity in the presence of interfering molecules, stability and reproducibility. The amperometric analysis of glucose showed a sensitivity of 3.1 μA μmol-1 l cm-2 and LOD of 6.48 μmol l-1 between 10 to 120 μmol l-1 at +500 mV. This detection limit is very good compared to other sensors used for the same purpose such as NiO-SWCNT (907 μmol l-1 ) [51]. Table 1 shows the different types of modified CPEs as well as the drugs detected and their detection medium, the limit of detection (LOD), concentration range and other characteristics. Table 1. Evaluation of electrochemical properties of modified CPE Electrode Analyte and matrix Method Linear dynamic range LOD Year / Ref poly-EBT/CPE MDH in Dilosyn syrup and human urine SWV 0.1- 50 μM 0.0257 μM 2020 / [26] CPE/nanozeolite type X Paracetamol and epinine LSV 0.5-70.0 μM Para = 0.2 μM Epi = 0.008 μM 2023 / [30] Ce-BTC MOF/IL/CPE KTC in pharmaceuti- cal and urine samples Chrono-ampe- rometry; DPV; CV; LSV 0.1-110.0 μM 0.04 μM S = 0.1342 μA μM-1 2023 / [31] AgNPs@CPE MTZ in milk and tap water - 1- 1000 μM 0.206 μM 2022 / [33] [10%FG/5%MW] CPE Ofloxacin in pharma- ceutical tablets human urine samples SW-AdAS 0.60 to 15.0 nM 0.18 nM 2019 / [41] MIP/CP ECL sensor Azithromycin urine and serum samples ECL 0.10 - 400 nM 0.023 nM 2018 / [45] CPEA/TiO2/UV AA in pharmaceutical tablets CV 150 - 850 nM 0.732 μM 2023 / [48] CuO MFs/Nafion/GC Glucose in real serum sample Amperometric 10 - 120 μM 6.48 μM S=3.1 μA μM-1 cm-2 2018 / [50] DMIP/CPE MTZ in Human Serum, Urine and drug DPV 0.4 - 200 μM 91 nM 2016 / [52] Fe3O4/ZIF-67 /ILCPE SMZ in urine and water DPV 0.01 - 520.0 M 5.0 nM 2021 / [53] poly-EBT: poly-eriochrome black T; Ce: cerium; BTC: benzene tricarboxylic acid; MOF : metal organic framework; Il : ionic liquids; AgNPs: silver nanoparticles; FG: Flake graphite; MW: multi-wall carbon nanotubes; MIP: molecularly imprinted polymer; ECL: electrochemiluminescence; CuO MFs: CuO micro flowers; DMIP: duplex molecularly imprinted polymer; ZIF-67: zeolitic imidazolate framework ; MDH: methdilazine hydrochloride; KTC: ketoconazole ; AA: ascorbic acid; MTZ: metronidazole; SMZ: sulfamethoxazole; S: sensitivity ADMET & DMPK 11(2) (2023) 151-173 Electrochemical sensors and biosensors for monitoring drugs and metabolites doi: https://doi.org/10.5599/admet.1709 155 The year of publication is indicated in each table in order to highlight the most recent publications and make them directly accessible to the reader. Electrodes based on modified glassy carbon for detecting various drugs and metabolites Glassy carbon is a type of carbon with an amorphous structure like glass and ceramics. Glassy carbon is an interesting material for electrochemical sensors because of its chemical resistance, low density and low electrical resistance. Glassy carbon electrodes (GCE) have also played an important function in electrochemical sensors and biosensors for drug analysis. The electron transfer rate at the GCE surface can be increased by various modifiers for the detection of medicines in diverse media. The GCE/ZnO@NDCS/GOx is an enzymatic biosensor used to analysis glucose in serum (cf. Table 2) [54]. The biosensor had a reproducible sensitivity of 231.7 μA mol-1 l cm-2 with a LOD of 6.3 μmol l-1 at 0.57 V. The sensor selectivity is demonstrated via the addition of some interfering potentials such as ascorbic acid, dopamine, fructose, uric acid and mannose (Man) with glucose, resulting in only a small increase in current for each species. Due to the easy transfer of electrons through this biosensor, the response time is very short (<3 s). The biosensor is well suited for glucose analysis with recovery between 99.73-100.14 % and retains about 95.3 % of the initial response during 50 days of storage in human blood. Acyclovir (ACV) (9-(2-hydroxyethoxymethyl)guanine) is a drug largely employed for the therapy of viral skin infections and neuritis [55-57]. Overdoses lead to adverse effects in patients [58,59]. The FeMoO4 compound is able to catalytically fix nitrogen [60] due to its interesting electrocatalytic property [61]. It also has interesting redox kinetics, making it a desirable material for electrode formation. Recently, Y. Wei et al. [62] prepared a sensor using graphene oxide composites loaded (ultrasonically) with ferrous molybdate (FeMoO4) for the analysis of ACV in pharmaceuticals by LSV. The oxidation peak of ACV is observed at 1.1 V. FeMoO4-GO/GCE and has two linearity intervals (0.1-10 and 10-100 μmol l-1 ) with a LOD of 20 nmol l-1. The reaction of ACV on the developed electrode is diffusion controlled with 2e- and 2H+ transferred. The active surface area of FeMoO4-GO/GCE is 1.59 times larger than that of FeMoO4/GCE, which clearly shows that the FeMoO4-GO improves the performance of the sensor. Y. Huang et al. [63] fabricated a biosensor to study the human umami taste receptor (hT1R1) and the umami substances, such as monosodium glutamate (MSG), using a multilayer material to modify the glassy carbon electrode (Figure 2). During the fabrication of this electrode, a human umami taste receptor (hT1R1) was attached to the layers formed by the AuNPs [64]. Horseradish peroxidase (HRP) is used for direct electron transfer to the multilayer material formed [65]. The researchers suggest that hT1R1 is likely a receptor used by the body to sense nitrogen, opening up a new way of studying nutrient and drug adsorption. In 2023, the same researchers [66] created another biosensor by attaching colon cancer and adjacent tissues to GCE to visualise the kinetics of responding to C and N nutrient receptors such as glucose and sodium lactate. In order to do this, they mixed solutions of starch gum with an aldehyde base and sodium alginate, which were spread over two microporous polycarbonate membranes into which the colon tissues were placed to build a layered assembly aligned to the GCE (Figure 3). Researchers found that the cells had different sensitivities to lactate, suggesting the possibility of using this nutrient to treat colon cancer. Colon cancer tissue is insensitive to lactate, whereas adjacent tissue is sensitive. CoCo2O4 nanorods embedded in hexagonal boron nitride are used to modify the GCE for ronidazole deter- mination [67]. The electrode synthesis presents a nano-LOD of 3 nmol l-1 between 0.01 and 1345 μmol l-1and a higher sensitivity of 5.845 µA μmol-1 l cm-2 using DPV. This sensitivity is explained by the enhanced adsorption and transport of mass ions via the formation of aggregates of hexagonal boron nitride (h-BN) and https://doi.org/10.5599/admet.1709 C. Laghlimi et al. ADMET & DMPK 11(2) (2023) 151-173 156 spinel cobalt oxide nanorods (CoCo2O4 NRs). CoCo2O4 NRs are used for their low cost, good stability and other properties. The h-BN, formed from boron and nitrogen, has a structure similar to graphene [68], which makes it an electroactive material [69,70]. Figure 2. Schematic of the multilayer modification of the GCE to study the electrochemical response of the human umami taste receptor hT1R1 towards umami substances such as sodium glutamate, disodium inosinate and disodium guanylate. Carried out at the base of the reference [63] with an order License ID of 1346500-1 Figure 3. Schematic of the multilayer modification of GCE to study the detection kinetics of C and N nutrient receptors. Based on the reference [66] Methotrexate (MTX) is a drug that inhibits the growth of tumour cells. It is applied for the treatment of certain types of cancer, notably breast cancer [71] and pulmonary cancer [72]. GO-Nafion-GCE sensor is constructed by dispersing graphite oxide in a solution containing Nafion-ethanol and then using it to modify the GCE [73]. Modified electrode stabilisation is achieved by using a CV of 0.1 V/s and in the potential between 0.5 and 1.2 V. GO-Nafion-GCE is able to detect methotrexate in MTX injection and urine with a LOD ADMET & DMPK 11(2) (2023) 151-173 Electrochemical sensors and biosensors for monitoring drugs and metabolites doi: https://doi.org/10.5599/admet.1709 157 of 9 nmol l-1 between 0.4 and 20 μmol l-1 , that is close to the LOD of detection of the same drug by 3DPG- CNT/GCE [74]. The electrolyte solution used for the analysis of MXT is perchloric acid (0.03 mol l-1). A mixed adsorption-diffusion phenomenon controls the reaction process of MXT on the electrode surface. Dopamine (DA) and paracetamol (PA) detection in synthetic urine was performed using a GCE- ERGO/polyCoTAPc electrode [75]. First, GCE was modified by electrochemically reduced graphene oxide (ERGO), which was simultaneously reduced and deposited from graphene oxide (GO). Finally, GCE- ERGO/polyCoTAPc was formed by electropolymerisation of cobalt (II) tetra-amino phthalocyanine. The developed electrode sensitivity was 1.32 µA mol-1 l cm-2 for PA and 8.39 µA mol-1 l cm-2 for DA. LOD is 0.10 µmol l-1 for PA and 0.095 µmol l-1 for DA using DPV. The modification of GCE by QDs-P6LC-PEDOT:PSS allowed the amoxicillin (AMX) analysis in synthetic urine, whole milk and pharmaceuticals [76]. AMX oxidation peak is 0.88V and the reaction process on QDs-P6LC- PEDOT:PSS/GCE is irreversible controlled by diffusion process with an equal number of proton and electron exchange. The researchers found that the alkaline medium facilitates the deprotonation of AMX during the oxidation reaction. Furthermore, the detection limit is found to be 50 nmol l-1 in the range 0.90-69 μmol l-1 . To form graphene (GR)-ZnO/GCE, X. Yue et al. [80] first polished the surface of GCE with a suspension of Al2O3 powder on a polishing cloth. Then, 8 µl of GR-ZnO nanocomposite suspension is used to modify the glassy carbon electrode. GR-ZnO/GCE was used for the simultaneous detection of sulfamethoxazole (SMZ) and trimethoprim (TMP) in urine and serum. SMZ and TMP oxidation peaks were successfully located at the Ep (SMZ) = 0.85 V and Ep (TMP) = 1.06 V by the DPV method, with a slight shift in peak potentials compared to those observed on the GCE. The researchers explained this by the ability of the GR to enhance ZnO nanorods conductivity and the reciprocal ability of the ZnO nanorods to avoid aggregation of the GR by reducing the van der Waals force. SMZ and TMP oxidation reactions are irreversible. They are controlled by adsorption. SMZ LOD is 0.4 μmol l-1 between 1-220 and 0.3 μmol l-1of TMP in the range of 1-180 μmol l-1 . The same drugs have been detected by GCE modified with GO and Ag nanoparticles (GC/rGO-AgNP) [81] using DPV with LOD (SMZ) = 0.6 μmol l-1 and LOD (TMP) = 0.4 μmol l-1 between 1.0 and 10.0 mmol l-1. In addition, SMZ and TMP exhibit irreversible oxidation peaks with ∆ETMP-SMZ= 1.14 - 0.92 = 0.22V. A further study has shown that the nickel ferrite/rGO (NiFe2O4/rGO) film is an excellent modifier of the GCE. Clenbuterol was analyzed in pig urine samples using the electrode produced [82]. The LOD is 0.17 µmol l-1 between 0.99 and 18.03 µmol l-1 using DPV. Due to the excellent selectivity of chitosan [83] and the high selectivity and ease of preparation of the MIP method [84], Y. Wu et al. [85] fabricated a sensor called MIP-MWCNTs/GCE for the detection of tryptophan (Trp), a possible cause of schizophrenia [86], by depositing a printed chitosan film on the MWCNT pre- modified GCE surface. This pre-modification with MWCNTs aims to enhance the response of molecularly imprinted polymer electrodes [87]. MWCNTs are a type of carbon nanotube with highly active sites due to the presence of more concentric tubes [88], which gives them high adhesive activity and good conductivity. Y. Wu et al. [85] demonstrated hydrogen bonding between chitosan and Trp using Fourier transform infrared (FTIR) spectroscopy. The extraction reagent (ethanol) used during the extraction process causes the chitosan film to deflate and etch, forming a porous structure capable of efficiently binding Trp molecules to these imprinted sites (Figure 4). The oxidation process of Trp involves the exchange of an identical number of e- and H+. Analysis of Trp in human serum by MIP-MWCNTs/GCE showed a very encouraging recovery between 96.5 and 102.5 %. In the presence of several interferents, the Trp peak is very intense compared to the other substances. The MIP-MWCNTs/GCE sensor has a low LOD (1 nmol l-1) compared to the GCE sensor modified with AuNP and MWCNTs [89]. https://doi.org/10.5599/admet.1709 C. Laghlimi et al. ADMET & DMPK 11(2) (2023) 151-173 158 Figure 4. Schematic of MIP-MWCNT/GCE preparation and electrochemical detection of Tryptophan. Based on the reference [85] Daunorubicin is an anticancer drug [90] that requires dose control [91] to avoid adverse effects, including cardiac arrest. H. Karimi-Maleh et al. [92] attempted to develop a glassy carbon electrode modified with a nanocomposite of Pt/SWCNTs and ds-DNA (biorecognition) to eventually form ds-DNA/Pt/SWCNTs/GCE. DVP shows a positive displacement of the daunorubicin oxidation peak (from 847 to 882 mV), confirming the mutual intercalation reaction of ds-DNA (guanine base) and daunorubicin. Furthermore, the equilibrium constant of the association is 5.044×103 mol-1 l with a nanometre LOD (1.0 nmol l-1) over a concentration between 4.0 nmol l-1 and 250.0 μmol l-1. Using the Fe3O4/MWCNT/GCE electrode, T. Bhengo et al. [93] obtained oxidation peaks of sulfa- methoxazole and trimethoprim at 910 and 1120 mV, respectively, with detection limits of 11.0 nmol l-1 for SMZ and 21.0 nmol l-1 for TMP using the DPV method. Similarly, Rajasekhar Chokkareddy et al. [94] deposited IL-f-ZnONPs@MWCNTson the clean surface of GCE by dispersing them in dimethylformamide (cf. Table 2).To prepare a solution containing SMZ, the researchers crushed two Sandoz Co-Trimoxazole (Pharmaceuticals) tablets and dissolved part of the powder in methanol. After shaking, the solution was refined and diluted with PBS (pH 6.0). The resulting solution was then added to the pharmaceutical urine samples. The cyclic voltammetry oxidation peak of the SMX was found to be at 620 mV. The reaction process on IL-f- ZnONPs@MWCNTs@GCE is diffusion controlled and the detection limit is 0.1 ng/ml in the range of 0.1-10 ng ml-1. In addition, Ag-MWCNT/MTOAC/GCE [95] was successfully used to detect SMX in Co-trimoxazole (mg per tablet), Cotrim paediatric and human urine by DPV. The samples were diluted with phosphate solution to reduce the matrix effect and then introduced into the electrochemical cell. The irreversible oxidation of the SMX is observed at +0.85 V and involves the exchange of an equal number of e- and H+ and is controlled by adsorption. On the other hand, the simultaneous analysis of ascorbic acid (AA), dopamine and uric acid in human urine was investigated using a GCE-based sensor modified with 2D titanium carbide nanoplatelets (MXene) [96]. All three analytes showed remarkably distinct oxidation peaks. AA was observed at 0.001 V, while dopamine and uric acid were at 210 mV and 330 mV, respectively. It should be noted that 2D titanium carbide (MXene) nanoplatelets are mentioned in the literature as having a high conductivity of 9880 S cm-1 [102], higher than ADMET & DMPK 11(2) (2023) 151-173 Electrochemical sensors and biosensors for monitoring drugs and metabolites doi: https://doi.org/10.5599/admet.1709 159 that of graphene. In particular, MXene groups, such as fluor, oxygen and hydroxyl, increase its hydrophilicity and allow rapid access to the analyte. It is therefore used as an ideal material for sensors [103]. Table 2 shows the different types of modified GCEs as well as the drugs detected and their detection medium, the limit of detection (LOD), concentration range and other characteristics. Table 2. Evaluation of electrochemical properties of modified GCE Electrode Analyte and matrix Method Linear dynamic range LOD Year / Ref GCE/ZnO@NDCS/GOx Glucose in hu- man blood serum CV; DPV;CAM 0.2 and 12 mM 6.3 μM S = 231.7 μA mM-1 cm-2 2018 / [54] FeMoO4-GO/GCE Acyclovir in drug samples LSV 0.1-10 μM 10-100 μM 20 nM 2022 / [62] CoCo2O4 NRs/h-BN/GCE Ronidazole water samples DPV 0.01-1345 µM 3 nM S = 5.845 µA µM-1 cm-2 2023 / [67] GO-Nafion-GCE MTX in metho- trexa e injection an u ine samples CV 0.4 -20 µM 9 nM 2019 / [73] GCE-ERGO/polyCoTAPc DA and Paracetamol in synthetic urine DPV - 0.10 µM for PA 0.095 µM for DA SPA=1.32 µA µM-1 cm-2 SDA= 8.39 µA µM-1 cm-2 2022 / [75] QDs-P6LC-PEDOT:PSS/GCE AX in synthetic urine, whole milk and pharmaceu- ticals SWV 0.90 to 69 µM 50 nM 2020 / [76] TiO2/CMK- 3/AuNPs/Nafion/GCE AX in pharma- ceutical product, mineral and envi- ronmental water CV LDR1= 0.5-2.5 μM LDR2 = 2.5-133 μM 0.3 µM S1 = 5071 μA mM−1 cm−2 S2 = 2971 μA mM−1 cm−2 2018 / [77] CB/DPH/GCE AX inbiological urine, lake and tap water SWV 2.0-18.8μM 0.12 µM 2018 / [78] NIM inbiological urine, lake and tap water SWV 0.30-5.0 μM 0.016 µM AuNPs/PdNPs/ErGO/GCE AX inhuman urine samples SWV 30.0-350.0μM 9.0 µM S = 0.0376 μA μM-1 2017 / [79] LMF human urine samples SWV 4-500 μM 81 nM S = 0.0759 μA μM-1 GR-ZnO/GCE SMZ and TMP in urine and human serum DPV LRDSMZ= 1-220 μM LRDTMP = 1-180 μM LODSMZ = 0.4 μM LRDTMP = 0.3 μM 2020 / [80] GC/rGO-AgNP SMZ and TMP in wastewaters samples DPV 1.0-10.0 mM LODSMZ = 0.6 μM LRDTMP = 0.4 μM 2017 / [81] MIP-MWCNTs/GCE Trp in the human serum samples. CV, SDLSVs 2.0 nM-0.2 μM 0.2 -10 μM 10-100 μM 1.0 nM 2020 / [85] ds-DNA/Pt/SWCNTs/GCE DRN in daunorubicin injection DVP 4.0 nM to 250.0 μM 1.0 nM 2021 / [92] IL-f-ZnONPs@MWCNTs@GCE SMZ pharmaceutical urine samples CV 0.1-10 ng ml-1 0.1 ng ml-1 2022 / [94] https://doi.org/10.5599/admet.1709 C. Laghlimi et al. ADMET & DMPK 11(2) (2023) 151-173 160 Electrode Analyte and matrix Method Linear dynamic range LOD Year / Ref Ag-MWCNT/MTOAC/GCE SMZ in pharmaceutical formulationsand human urine DPV 0.05-70 μM 0.01 μM 2018 / [95] Ti-C-Tx/GCE AA in human urine samples CV, DPV 100-1000μM 4.6 μM 2021 / [96] DA in human urine samples CV, DPV 0.5-50 μM 0.06 μM UA in human urine samples CV, DPV 0.5-4 μM 100-1500 μM 0.075 μM PVP-GR/GCE AA in human urine samples LSV 4-1000 μM 0.80μM 2020 / [97] DA in human urine samples LSV 0.02-100 μM 0.002 μM UA in human urine samples LSV 0.04-100 μM 0.02 μM rGO-ZnO/GCE AAin real plasma and urine samples DPV 50-2350μM 3.71μM 2016 / [98] UA in real plasma and urine samples DPV 1-70 μM 0.33 μM DA in real plasma and urine samples DPV 3-330 μM 1.08 μM 3D-HPG/PTH/GCE MTZ in pharma- ceutical and real wate samples CV; DPV 0.05-70 μM 70−500 μM 1 nM 2018 / [99] Polydopamine/MWCNTseCOO H nanocomposites/GCE MTZ in pharma- ceutical and bio- logical samples DPV 5-5000 μM 0.25 μM 2018/ [100] Ag-MWCNT/MTOAC/GCE SMZ in pharmaceutical formulations and human urine DPV 0.05-70 μM 0.01 μM 2018 / [101] NDCS: nitrogen-doped carbon sheets; GOx: glucose oxidase; FeMoO4-GO: ferrous molybdate-graphene oxide; CoCo2O4 NRs: spinel cobalt oxide nanorods; h-BN: hexagonal boron nitride; ERGO: electrochemically reduced graphene oxide; polyCoTAPc: polymer of cobalt (II) tetra-amino phthalocyanine; QDs: cadmium telluride quantum dots; P6LC: Printex 6L Carbon; PEDOT: poly(3,4-ethylenedioxythiophene; PSS: polystyrene sulphonate film; CMK-3: mesoporous carbon CMK-3, AuNPs: gold nanoparticles; DPH: dihexadecyl hydrogen phosphate; CB: carbon black; PdNPs: palladium nanoparticles; GR: graphene; MIP: molecularly imprinted polymer; ds-DNA: guanine-based; IL-f-ZnONPs: ionic liquid functionalised zinc oxide nanoparticles; MTOAC: methyltrioctyl ammonium chloride; Ti-C-Tx: titanium carbide (MXene) nanosheets; PVP: polyvinylpyrrolidone; rGO: Reduced graphene; 3D: three-dimensional; HPG: graphene-like carbon architecture; PTH: polythionine; MWCNTseCOOH: carboxylic MWCNT; RNZ: ronidazole; MTX: methotrexate; DA: dopamine; AX: Amoxicillin, NIM: nimesulide; LMF: lomefloxacin; SMZ: sulfamethoxazole; TMP: trimethoprim; Trp: tryptophan; DNR: daunorubicin, AA: acide ascoorbic; DA: dopamine; UA: uric acid; MTZ: metronidazole; S: sensitivity; CAM: chronoamperometry. Electrodes based on modified graphene oxide for detecting various drugs and metabolites Graphene oxide reduced (rGO) is obtained by thermal, electrochemical or chemical treatment of graphene oxide using substances such as NaBH4 or aluminium powder. This reduction removes oxidised functional groups (which increases the sensitivity of the GO and makes it insulating) and creates a defect structure (active sites) characteristic of rGO. These properties have enabled rGO to have high electrochemical activity compared to graphene and graphene oxide. F. Zhou et al. [104] successfully fabricated a Nafion/GOx/Au-ZnO/rGO/ITO electrode. The researchers found that the rate and efficiency of electron transfer were augmented by the deposition of AuNPs and UV irradiation, allowing a significant increase in glucose sensitivity (cf. Table 3). LOD of this sensor is 0.2 μmol l-1 https://www.sciencedirect.com/topics/materials-science/titanium-carbide https://www.sciencedirect.com/topics/materials-science/nanosheet https://www.sciencedirect.com/topics/chemistry/chronoamperometry ADMET & DMPK 11(2) (2023) 151-173 Electrochemical sensors and biosensors for monitoring drugs and metabolites doi: https://doi.org/10.5599/admet.1709 161 by amperometric method between 0 and 9.5 mmol l-1 with a higher Michaelis-Menten constant (15.54 mmol l-1) than that of non-UV irradiated Nafion/GOx/Au-ZnO/rGO/ITO. The sensors have exceptional detection accuracy for blood glucose measurement. Regorafenib (REG) or Regonix is an anticancer drug used in several types of cancer [105,106]. Monitoring the concentration of REG in serum or blood seems to be of great interest as doses above certain limits can cause adverse effects on vital organs of the body [107]. REG is detected in real samples by Pd-Ru/rGO using the DPV technique [108] (cf. Table 3). The acidic graphene neutrality is obtained from pomegranate peel extract (PPE). In fact, the redox current peak is linear with v (v = potential sweep rate), indicating that the REG reaction occurring at Pd-Ru/rGO is diffusion controlled, with a constant heterogeneous charge transfer rate equal to K0 = 2.29 s-1, calculated based on Laviron's equation (cf. Eq. (1), (2) and (3)) [109]. Furthermore, the REG oxidation involves the exchange of 2e- and 2H+. ( ) a 0 2.3 log 1 RT v Ep E nF = + − (1) c 0 2.3 logRT v Ep E nF = + (2) ( ) ( ) ( ) s 1 log log 1 1 log log 2.3 nF EpRT k nFv RT       −   = − + − − −    (3) Table 3 shows the different types of modified rGOs as well as the drugs detected and their detection medium, limit of detection (LOD), concentration range and other characteristics. Table 3. Evaluation of electrochemical properties of modified rGO Electrode Analyte and matrix Method Linear dynamic range LOD Other characteristics Year/Ref. Nafion/GOx/Au- ZnO/rGO/ITO glucose in blood Ampero- metric 0-9.5 mM 0.2 μM S = 10.93 μA mM-1 cm-2 2020 / [104] Pd-Ru/rGO Regorafenib in human blood and plasma phar- maceutical formulation DPV 0.5-300 nM 1.6 nM kS = 2.29 s-1 2022 / [108] rGO/TMU-22 MOF Levodopa in human urine and tablet samples CV; SWV 0.1-85 μM 0.1-85 µM 25 nM S = 0.58 µA µM−1 kS = 7.7 s−1 2020 / [110] AuNPs : gold nanoparticles ; GOx: glucose oxidase; rGO: reduced graphene oxide;ITO: indium tin oxide ; TMU-22 MOF: metal-organic framework; Pd-Ru: palladium/ruthenium nanoparticles; S: sensitivity Electrodes based on modified screen-printed carbon electrodes for detecting various drugs and metabolites The screen-printed electrode (SPE) consists of a substrate (PVC, ceramic) onto which miniature electrodes can be printed using ink based on conductive materials. Various substances are employed to increase the sensitivity of the screen-printed electrode, including fullerenes (C60) and iron(III) nanoparticles (Fe3O4NP). The antibiotic drug furaltadone (FLT) was detected by a screen-printed carbon electrode (SPCE) decorated with Cu/Ni/TiO2/MWCNTs nanocomposites [111]. FLT reduction peak on Cu/Ni/TiO2/MWCNTs nanocomposites was located at -470 mV with a significant current density of -39.17 μA. The reduction of the R-NO2 group to R- NHOH was performed by electron transfer from HOMO to LUMO in the fabricated electrode and the LOD is equal to 0.0949 μmol l-1 with a high sensitivity of 1.9288 μA μmol-1 l cm-2 in water. We also find that erythron- mycin (ERY) is detected by sodium dodecyl sulfate-modified SPCEs (SPCEs/SDS) by flow injection analysis with amperometric detection in PBS of pH 8.5 [112]. Peak detection of ERY is 0.7 V with an LOD of 0.19 μmol l-1. A SPE coated with C60, rGO and Nafion (NF) is used to analysis antibiotic metronidazole (MTZ) in real samples (cf. Table 4) [113]. Reduction of GO with NaBH4 results in the disappearance of oxygen groups. Fullerenes are used because of their ability to operate at low potentials, which is advantageous for a sensor https://doi.org/10.5599/admet.1709 C. Laghlimi et al. ADMET & DMPK 11(2) (2023) 151-173 162 to avoid interference effects [114,115]. To stabilise the response of the C60-rGO-NF/SPE, the researchers applied two cyclic voltammetry cycles between 0 and -1500 mV at v = 20 mV s-1 in a 1 mol l-1 electrolyte solution of KOH and then the electrode was placed in PBS (Neutre) to apply v = 50 mV s-1 (scan rate) between 550 and -50 mV. The MTZ reduction peak observed at -0.9 V, representing the exchange of 4e- and 4H+. The intensity of the MTZ reduction peak on C60-rGO-NF/SPE is five times higher than that observed on SPE. This sensitivity is explained by the porosity of C60, rGO and NF, which increases the surface area of C60-rGO- NF/SPE. The LOD by SWV is 0.21 μmol l-1in the range 0.25-34.0 μmol l-1. GP-CAc/PVC is a screen-printed electrode (SPE) [116], estimated to cost 0.016 $ per unit compared to 7 $ for a commercial electrode, used to determine levodopa (L-dopa) in two commercial drugs, Parkidopa® and Ekson®. These drugs are prescribed to treat Parkinson's disease by reducing the severity of symptoms. To maintain the conductive material of the electrode, researchers have used polymeric materials such as cellulose [117], polyvinyl [118], epoxy glue, nail polish [119] and cellulose acetate (CAc) [120]. In this case, the conductive ink for the electrode is obtained by mixing cellulose acetate and graphite powder in attendance of acetone/cyclohexanone (apolar solvents). The adhesive stencil is used to draw the relief of three electrodes, which are then attached to the PVC. Conductive ink is then applied to the stencil to print the electrodes on the PVC. The stencil is immediately removed to allow the ink to run off. The GP-CAc/PVC has an electroactive surface area of 0.48 cm2 with a sensitivity of 0.101 μA μmol-1 l owing to the significant porosity of the cellulose acetate. The detection limit by SWV is 0.06 μmol l-1 between 8.00 and 100 μmol l-1. The researchers found that at pH 5 (pH ≥ 5.0), L-dopa exhibits an irreversible reaction with an oxidation peak located at 0.25 V caused by exchanging the 2H+ and 2e-. Furthermore, at pH 2 (pH ≥ 4.0), the behaviour of L-dopa changes towards a reversible system with Ep(oxy) = 0.27V and Ep(red) = -0.01V. On the other hand, SPE modified with the CuO/Co3O4 nanocomposite integrated MWCNTs [121] has a very low LOD of 0.223 pmol l-1 between 10-12 and 10-2 mol l-1 range for urea determination by electrochemical impedance spectroscopy (EIS) (Fig. 5). This performance is due to the combination of CuO and Co3O4, which have higher electronic conductivity than Co3O4 or CuO alone. Figure 5. Schematic of CuO/Co3O4@MWCNTs preparation and electrochemical detection of urea. Based on the reference [121] ADMET & DMPK 11(2) (2023) 151-173 Electrochemical sensors and biosensors for monitoring drugs and metabolites doi: https://doi.org/10.5599/admet.1709 163 Table 4 shows the different types of modified SPEs as well as the drugs detected and their detection medium, limit of detection (LOD), concentration range and other characteristics. Table 4. Evaluation of electrochemical properties of modified SPE Electrode Analyte and matrix Method Linear dynamic range LOD / Other characteristics Year / Ref. Cu/Ni/TiO2/MWCNTs/SPCE FLT in water CV; DPV 10-150 μM 0.0949 μM S = 1.9288 μA μM-1 cm-2 2022 / [111] SPCEs/SDS ERY in water Amperometric 1-15 mg l-1 0.19 μM 2019 / [112] C60-rGO-NF/SPE MTZ in serum and urine samples SWV 0.25-34.0 μM 0.21 μM 2021 / [113] GP-CAc/PVC Levodopa in two commercial drugs SWV 8-100 μM 0.06 μM 2021 / [116] CuO/Co3O4@MWCNTs/SPE Urea EIS 10-12-10-2 M 0.223 pM 2023 / [121] Nano-Au/MWNTs-ZnO/SPE MTX in whole blood samples and pharmaceutical SWV 0.02-1.00 μM 10 nM 2014 / [122] EPI in whole blood samples and pharmaceutical SWV 0.005-0.2 μM 2.5 nM MWCNT-AONP/SPCE AA in fresh oranges SWV 160-640 nM 140 nM 2022 / [123] SPCE/CV-Fe3O4NP AA SWV 10-100 μM 15.7μM 2021 / [124] SDS: sodium dodecyl sulfate ; NF: Nafion; C60: fullerene ; CAc: cellulose acetate; GP: graphite powder; PVC: polyvinyl chloride; AONP: antimony oxide nanoparticle; CV: Callistemon viminalis; Fe3O4NP: Iron(III) nanoparticle; FLT: furaltadone; ERY:erythromycin; MTZ:metronidazole ; MTX : methotrexate; EPI : epirubicin; AA: ascorbic acid. Conclusions and perspectives This review discusses the different types of recently published electrochemical sensors for the detection of drugs and metabolites in pharmaceutical and biological samples. CPE has a very large electroactive surface area, which can be renewed for a variety of applications. In addition, it has low ohmic resistance and high stability, reproducibility and lifetime, giving it the ability to analyse various drugs, revealing the oxidation and reduction processes involved due to its wide potential range. The sensitivity and speed of analysis of CPE can be significantly improved by modifying them with conductive materials. Various modifiers were used, including organic modifiers like Poly-Eriochrome Black T (Poly-EBT), benzene tricarboxylic acid (BTC), metal- organic frameworks (MOFs) or inorganic modifiers like silver nanoparticles (AgNPs), CuO microfragments (CuO MFs) and TiO2. On the other hand, the improvement of CPE can be achieved by using a carbon nanomaterial modifier such as flake graphite (FG), multi-wall carbon nanotubes (MWCNTs) and carbon quantum dots (CQDs). The modified carbon past electrode has been used for the determination of various drugs such as methdilazine hydrochloride (MDH), ketoconazole (KTC), metronidazole (MTZ), sulfametho- xazole (SMZ) in diverse samples such as human urine, pharmaceutical formulations, plasma and serum. Modification with silver nanoparticles is also encouraged due to their biocompatibility, sensitivity, stability and ability to increase peak intensity due to their high conductivity. Similarly, metal oxide nanomaterials such as CuO MFs have an essential contribution to the performance of CPE owing to their wide surface area. L. Hu et al. fabricated a selective electrochemiluminescence (ECL) sensor by modifying an CPE with a MIP. The sensor showed a very low LOD of 0.023 nmol l-1 to analyse azithromycin (AZI) in real samples.The very low LOD of 0.18 nmol l-1 is also found by a CPE modified with lamellar graphite and MWCNT for the analysis of ofloxacin in pharmaceutical tablets and human urine samples. Similarly, CPE modified with Fe3O4/ZIF- 67/ILCPE nanocomposite was applied for the analysis of SMZ in urine and water with a LOD of 5.0 nmol l-1. https://doi.org/10.5599/admet.1709 C. Laghlimi et al. ADMET & DMPK 11(2) (2023) 151-173 164 The transfer rate of electrons at the surface of glassy carbon electrodes (GCE) can be increased by various modifiers such as nitrogen-doped carbon sheets, glucose oxidase, cobalt (II) polymer tetraamino phthalocya- nine (polyCoTAPc), methyltrioctyl ammonium chloride (MTOAC) and guanine (ds-DNA). In addition, inorganic modifiers such as iron molybdate (FeMoO4), spinel cobalt oxide nanorods (CoCo2O4 NRs), hexagonal boron nitride (h-BN), cobalt (II) tetra-aminophthalocyanine polymer (polyCoTAPc), cadmium telluride quantum dots (QDs) and titanium carbide (MXene) nanosheets (Ti-C-Tx) have been used for the same purpose. Similarly, a variety of carbon types have been reported in the literature to improve GCE sensitivity, including GO, electro- chemically reduced graphene oxide (ERGO), Printex 6L carbon (P6LC), CMK-3 mesoporous carbon (CMK-3), carbon black (CB), graphene (GR), reduced graphene (rGO), graphene-like carbon architecture (HPG) and carboxylic MWCNT (MWCNTseCOOH). The modified GCE was used to determine the drugs in the different matrices. The drugs analysed were: ronidazole, methotrexate, dopamine, amoxicillin, nimesulide, lomefloxacin, sulphamethoxazole, trimethoprim, tryptophan and daunorubicin. Platinum nanoparticles have high electro- catalytic properties. Similarly, DNA nanostructures have high selectivity and affinity for the analyte, which can be used to modify the GCE used for electrochemical drug analysis. The conductivity of the glassy carbon elec- trodes can be increased by using polymers such as polyaniline (PANI). The combination of various modifiers with high conductivity electrochemical materials such as MIPs, MWCNTs, SWCNTs, ds-DNA and Pt has further increased the sensitivity of the sensors. MIP-MWCNTs/GCE and ds-DNA/Pt/SWCNTs/GCE showed a very low LOD of 1 nmol l-1 for drug determination. In addition, the modification with 3D-HPG/PTH shows a similar nano- metric detection limit. In 2023, a group of researchers successfully fabricated a glassy carbon electrode modified with CoCo2O4 NRs/h-BN used to analysis the MTR in pharmaceuticals, with a sensitivity of 5.845 µA µmol-1 l cm-2 and a LOD of 3 nmol l-1. AuNPs have been employed as modifiers for rGO due to their considerable catalytic activity, biocompa- tibility and high electrochemical potential, which allows the redox process generated at the electrode surface to be detected. Other materials are employed to improve the sensitivity of rGOs, including glucose oxidase, indium tin oxide (ITO), metal-organic framework (MOF TMU-22) and palladium/ruthenium nanoparticles (Pd-Ru). The modification of rGO with Pd-Ru resulted in a very low LOD of 1.6 nmol l-1 for the determination of regorafenib in various samples like human blood, plasma and pharmaceutical formulations. A variety of conductive compounds are available to increase the sensitivity of the screen-printed elec- trode, like sodium dodecyl sulphate (SDS), Nafion (NF), fullerene (C60), cellulose acetate, graphite powder, antimony oxide nanoparticles (AONP) and iron (III) nanoparticles (Fe3O4NP). In addition, multi-wall carbon nanotubes (MWNTs) have a high active surface area and conductivity. The specific structure of these nanomaterials gives them the potential to be an interesting candidate for drug detection electrochemical sensors. The combination of metal oxide and carbon nanotubes significantly increases the sensitivity of SPE. Recently, H. S. Magar et al. fabricated an SPE using a combination of CuO/Co3O4 and MWCNTs. The sensor was used for urea analysis with a very low LOD of 0.223 pmol l-1. Not all electrode types mentioned in this paper reported this value. In addition, a LOD of 2.5 nmol l-1 was found for nano-Au/MWNTs-ZnO/SPE used to analysis EPI in whole blood and pharmaceutical samples. The use of nanomaterials and metal oxides seems promising to improve the sensitivity of the electrodes, but there are many problems, such as difficulties in fabrication, handling and characterisation, which encourage investment in finding simpler fabrication methods or extraction from plants and animal secretions. Acknowledgements: We are grateful to Mr. Haroun Laghlimi (My dear son), Mrs. Fatna NÂna and Mrs. Ikram Ait Lebbadfor their encouragement and support. ADMET & DMPK 11(2) (2023) 151-173 Electrochemical sensors and biosensors for monitoring drugs and metabolites doi: https://doi.org/10.5599/admet.1709 165 Conflict of interest: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. List of abbreviations C60 Buckminsterfullerenes CA Chronoamperometry CPE Carbon paste electrodes CPEA Carbon clay paste electrode CV Cyclic voltammetry DPV Differential pulse voltammetry EBT Eriochrome black T ECL Electrochemiluminescence EIS Electrochemical impedance spectroscopy ERGO Electrochemically reduced graphene oxide FG Flake graphite FTIR Fourier transform infrared GCE Glassy carbon electrode GO Graphene oxide GP Graphite powder GR Graphene HOMO Highest occupied molecular orbital HPLC-DAD High performance liquid chromatography-diode array detector LOD Limit of detection LSV Linear scan voltammetry LUMO Lowest unoccupied molecular orbital MIP Molecularly imprinted polymer MWCNTs Multi-wall carbon nanotubes MXene M: transition metal, X: carbon and/or nitrogen, ene: similarity to graphene NF Nafion PBS Phosphate-buffered saline PVC Polyvinyl chloride rGO Reduced graphene oxide SDLSV Second-order derivative linear sweep voltammetry SPC Screen-printed carbon SPCE Screen-printed carbon electrode SW-AdAS Square wave adsorptive anodic stripping voltammetry SWCNT Single-walled carbon nanotubes SWV Square wave voltammetry TLC-densitometric Thin layer chromatography densitometric References [1] S.B. 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