manuscript doi: 10.5599/admet.3.4.261 296 ADMET & DMPK 3(4) (2015) 296-297; doi: 10.5599/admet.3.4.261 Open Access : ISSN : 1848-7718 http://www.pub.iapchem.org/ojs/index.php/admet/index Editorial pKa values in solubility determination using the Henderson- Hasselbalch equation Derek Reynolds and Kin Tam ADMET & DMPK Editorial Board, email: admet_dmpk@iapchem.org. Published: December 30, 2015 In this Journal we publish only high quality peer reviewed scientific papers but sometimes not all scientists can agree with all aspects of a particular paper. We wish to encourage healthy scientific debate and when appropriate it is right to publish alternative interpretations of reported experiments. Alex Avdeef [1] and Samuel Yalkowsky [2] have independently studied the solubility and ionization properties of an investigational drug (NSC-639829). In the study conducted by Avdeef and coworkers, the pKa value derived from Henderson-Hasselbalch (HH) equation was 4.70, which was different from the value of 3.76 as determined experimentally by the Yasuda-Shedlovsky plot using the pKa values obtained from co-solvent UV titrations. The authors then invoked an ionization-precipitation-aggregation model by assuming the formation of a positively charged dimer to generate the theoretical solubility-pH profile. In their implementation, the pKa was fixed at 3.76, while the S0, and the aggregation equilibrium constant were treated as adjustable parameters. To this end, the authors obtained a reasonably good fit between the model and the experiment. The S0 value as determined using this approach appeared to be in line with the previously reported value [2]. In this issue, Yalkowsky et al. [3] published a note to comment on the approach that Avdeef et al. [1] developed in the study of NSC-639829. In particular, they have pointed out that the pKa of 4.70 for NSC- 639829 was the correct value in the solubility determination study, and the analysis carried out by Avdeef et al. [1] was problematical. Yalkowsky et al. have highlighted that pKa is a concentration dependent parameter, which was supported by several literature examples [3]. Apparently, Yalkowsky et al. have put forward some compelling arguments to justify their points of view in case of NSC-639829. Generally speaking, pKa is not an absolute molecular constant because it depends very much on the medium, conditions and ionic strength of determination. For low sample concentration and/or in the presence of co- solvent, the formation of aggregate in pKa determination is less favorable. In case of NSC-639829, the pKa determination was accomplished by long range extrapolation from co-solvent pKa data (22-41 % methanol- water mixtures) [1]. Again, this may introduce some uncertainty to the extrapolated pKa value. It may be difficult to make direct comparison between the pKa determined independently and the pKa derived from the HH equation since the chemical/physical environments are unlikely to be the same in these two experiments. In our opinion, Avdeef et al. [1] have developed very powerful software to analyze complex ionization- precipitation-aggregation system, which we should applaud. On the other hand, Yalkowsky et al. pointed http://www.pub.iapchem.org/ojs/index.php/admet/index mailto:admet_dmpk@iapchem.org ADMET & DMPK 3(4) (2015) 296-297 Editorial on Henderson-Hasselbalh equation doi: 10.5599/admet.3.4.261 297 out that effectively what the software does in the case of NSC-639829 was adding an arbitrary factor of 0.94 to bring the pKa to 4.70. We see that these are just two different approaches to interpret the same dataset. Perhaps the aggregate formation may well be true as proposed by Avdeef et al. [1]. However, it is always difficult to prove the formation of the positively charged dimer in the equilibrium model solely based on mass balance calculation. To confirm the validity of the model, it is necessary to obtain independent structural or spectroscopic evidence. Although this discussion centers around just one compound it is part of a wider debate regarding the applicability of the HH equation to the study of solubility where complications are likely to arise due to presence of aggregate species in solution. All the authors of these papers have made significant contributions to the field. We hope that many scientists with an interest in solubility and ionization will read both papers and learn from the differing views of these eminent researchers. References [1] G. Butcher, J. Comer, A. Avdeef, ADMET & DMPK 3(2) (2015) 131-140. [2] N. Jain, G. Yang, S.E. Tabibi, S.H. Yalkowsky, Int. J. Pharm. 225 (2001) 41-47. [3] S. Yalkowsky, R. Patel, D. Al-Antary, ADMET & DMPK 3(4) (2015) 358-361. ©2015 by the authors; licensee IAPC, Zagreb, Croatia. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/) http://creativecommons.org/licenses/by/3.0/