id sid tid token lemma pos admet-389 1 1 manuscript manuscript PROPN admet-389 1 2 doi doi PROPN admet-389 1 3 : : PUNCT admet-389 1 4 10.5599 10.5599 NUM admet-389 1 5 / / SYM admet-389 1 6 admet.5.2.389 admet.5.2.389 NOUN admet-389 1 7 135 135 NUM admet-389 1 8 admet admet X admet-389 1 9 & & CCONJ admet-389 1 10 dmpk dmpk PROPN admet-389 1 11 5(2 5(2 NUM admet-389 1 12 ) ) PUNCT admet-389 1 13 ( ( PUNCT admet-389 1 14 2017 2017 NUM admet-389 1 15 ) ) PUNCT admet-389 1 16 135 135 NUM admet-389 1 17 - - SYM admet-389 1 18 145 145 NUM admet-389 1 19 ; ; PUNCT admet-389 1 20 doi doi NOUN admet-389 1 21 : : PUNCT admet-389 1 22 10.5599 10.5599 NUM admet-389 1 23 / / SYM admet-389 1 24 admet.5.2.389 admet.5.2.389 NOUN admet-389 1 25 open open ADJ admet-389 1 26 access access NOUN admet-389 1 27 : : PUNCT admet-389 1 28 issn issn PROPN admet-389 1 29 : : PUNCT admet-389 1 30 1848 1848 NUM admet-389 1 31 - - SYM admet-389 1 32 7718 7718 NUM admet-389 1 33 http://www.pub.iapchem.org/ojs/index.php/admet/index http://www.pub.iapchem.org/ojs/index.php/admet/index NOUN admet-389 1 34 original original ADJ admet-389 1 35 scientific scientific ADJ admet-389 1 36 paper paper NOUN admet-389 1 37 fluorescent fluorescent NOUN admet-389 1 38 organic organic ADJ admet-389 1 39 cations cation NOUN admet-389 1 40 for for ADP admet-389 1 41 human human ADJ admet-389 1 42 oct2 oct2 NOUN admet-389 1 43 transporters transporter NOUN admet-389 1 44 screening screen VERB admet-389 1 45 : : PUNCT admet-389 1 46 uptake uptake ADJ admet-389 1 47 in in ADP admet-389 1 48 cho cho NOUN admet-389 1 49 cells cell NOUN admet-389 1 50 stably stably ADV admet-389 1 51 expressing express VERB admet-389 1 52 hoct2 hoct2 PROPN admet-389 1 53 malachy malachy PROPN admet-389 1 54 c. c. PROPN admet-389 1 55 ugwu1 ugwu1 PROPN admet-389 1 56 , , PUNCT admet-389 1 57 2 2 NUM admet-389 1 58 , , PUNCT admet-389 1 59 ryan ryan PROPN admet-389 1 60 pelis3 pelis3 PROPN admet-389 1 61 , , PUNCT admet-389 1 62 charles charles PROPN admet-389 1 63 o. o. PROPN admet-389 1 64 esimone2 esimone2 PROPN admet-389 1 65 & & CCONJ admet-389 1 66 remigius remigius PROPN admet-389 1 67 u. u. PROPN admet-389 1 68 agu1 agu1 PROPN admet-389 1 69 * * SYM admet-389 1 70 1 1 NUM admet-389 1 71 biopharmaceutics biopharmaceutics NOUN admet-389 1 72 and and CCONJ admet-389 1 73 drug drug NOUN admet-389 1 74 delivery delivery NOUN admet-389 1 75 lab lab NOUN admet-389 1 76 college college NOUN admet-389 1 77 of of ADP admet-389 1 78 pharmacy pharmacy NOUN admet-389 1 79 5968 5968 NUM admet-389 1 80 college college NOUN admet-389 1 81 street street NOUN admet-389 1 82 , , PUNCT admet-389 1 83 po po PROPN admet-389 1 84 box box PROPN admet-389 1 85 15000 15000 NUM admet-389 1 86 , , PUNCT admet-389 1 87 halifax halifax NOUN admet-389 1 88 , , PUNCT admet-389 1 89 ns ns NUM admet-389 1 90 b3h b3h NOUN admet-389 1 91 4r2 4r2 NUM admet-389 1 92 , , PUNCT admet-389 1 93 canada canada PROPN admet-389 1 94 2 2 NUM admet-389 1 95 department department NOUN admet-389 1 96 of of ADP admet-389 1 97 pharmaceutical pharmaceutical NOUN admet-389 1 98 microbiology microbiology NOUN admet-389 1 99 & & CCONJ admet-389 1 100 biotechnology biotechnology NOUN admet-389 1 101 faculty faculty NOUN admet-389 1 102 of of ADP admet-389 1 103 pharmaceutical pharmaceutical PROPN admet-389 1 104 sciences science NOUN admet-389 1 105 nnamdi nnamdi PROPN admet-389 1 106 azikiwe azikiwe PROPN admet-389 1 107 university university PROPN admet-389 1 108 , , PUNCT admet-389 1 109 near near ADP admet-389 1 110 nafdac nafdac PROPN admet-389 1 111 zonal zonal ADJ admet-389 1 112 lab lab NOUN admet-389 1 113 , , PUNCT admet-389 1 114 agulu agulu PROPN admet-389 1 115 , , PUNCT admet-389 1 116 anambra anambra PROPN admet-389 1 117 state state PROPN admet-389 1 118 , , PUNCT admet-389 1 119 nigeria nigeria PROPN admet-389 1 120 . . PUNCT admet-389 2 1 3 3 NUM admet-389 2 2 department department NOUN admet-389 2 3 of of ADP admet-389 2 4 pharmacology pharmacology NOUN admet-389 2 5 faculty faculty NOUN admet-389 2 6 of of ADP admet-389 2 7 medicine medicine PROPN admet-389 2 8 dalhousie dalhousie PROPN admet-389 2 9 university university PROPN admet-389 2 10 p.o p.o PROPN admet-389 2 11 . . PROPN admet-389 2 12 box box PROPN admet-389 2 13 15000 15000 NUM admet-389 2 14 
 
 SPACE admet-389 2 15 halifax halifax PROPN admet-389 2 16 , , PUNCT admet-389 2 17 ns ns ADJ admet-389 2 18 
 
 SPACE admet-389 2 19 b3h b3h NOUN admet-389 2 20 4r2 4r2 NUM admet-389 2 21 * * PUNCT admet-389 2 22 corresponding correspond VERB admet-389 2 23 author author NOUN admet-389 2 24 : : PUNCT admet-389 2 25 e e NOUN admet-389 2 26 - - NOUN admet-389 2 27 mail mail NOUN admet-389 2 28 : : PUNCT admet-389 2 29 remigius.agu@dal.cab remigius.agu@dal.cab NOUN admet-389 2 30 ; ; PUNCT admet-389 2 31 tel tel PROPN admet-389 2 32 . . PUNCT admet-389 2 33 : : PUNCT admet-389 3 1 +1 +1 PROPN admet-389 3 2 - - PUNCT admet-389 3 3 902 902 NUM admet-389 3 4 - - PUNCT admet-389 3 5 494 494 NUM admet-389 3 6 - - NUM admet-389 3 7 2092 2092 NUM admet-389 3 8 ; ; PUNCT admet-389 3 9 fax fax NOUN admet-389 3 10 : : PUNCT admet-389 3 11 +1 +1 PROPN admet-389 3 12 - - PUNCT admet-389 3 13 902 902 NUM admet-389 3 14 - - PUNCT admet-389 3 15 494 494 NUM admet-389 3 16 - - SYM admet-389 3 17 1396 1396 NUM admet-389 3 18 received receive VERB admet-389 3 19 : : PUNCT admet-389 3 20 april april PROPN admet-389 3 21 28 28 NUM admet-389 3 22 , , PUNCT admet-389 3 23 2017 2017 NUM admet-389 3 24 ; ; PUNCT admet-389 3 25 revised revise VERB admet-389 3 26 : : PUNCT admet-389 3 27 june june PROPN admet-389 3 28 05 05 NUM admet-389 3 29 , , PUNCT admet-389 3 30 2017 2017 NUM admet-389 3 31 ; ; PUNCT admet-389 3 32 published publish VERB admet-389 3 33 : : PUNCT admet-389 3 34 june june PROPN admet-389 3 35 22 22 NUM admet-389 3 36 , , PUNCT admet-389 3 37 2017 2017 NUM admet-389 3 38 abstract abstract NOUN admet-389 3 39 the the DET admet-389 3 40 aim aim NOUN admet-389 3 41 of of ADP admet-389 3 42 this this DET admet-389 3 43 study study NOUN admet-389 3 44 was be AUX admet-389 3 45 to to PART admet-389 3 46 assess assess VERB admet-389 3 47 the the DET admet-389 3 48 suitability suitability NOUN admet-389 3 49 of of ADP admet-389 3 50 amiloride amiloride NOUN admet-389 3 51 , , PUNCT admet-389 3 52 rhodamine rhodamine NOUN admet-389 3 53 6 6 NUM admet-389 3 54 g g NOUN admet-389 3 55 and and CCONJ admet-389 3 56 rhodamine rhodamine NOUN admet-389 3 57 123 123 NUM admet-389 3 58 as as ADP admet-389 3 59 nonradioactive nonradioactive ADJ admet-389 3 60 substrates substrate NOUN admet-389 3 61 for for ADP admet-389 3 62 characterizing characterize VERB admet-389 3 63 hoct2 hoct2 NOUN admet-389 3 64 using use VERB admet-389 3 65 cho cho NOUN admet-389 3 66 cells cell NOUN admet-389 3 67 . . PUNCT admet-389 4 1 the the DET admet-389 4 2 uptake uptake ADJ admet-389 4 3 characteristics characteristic NOUN admet-389 4 4 of of ADP admet-389 4 5 these these DET admet-389 4 6 compounds compound NOUN admet-389 4 7 were be AUX admet-389 4 8 compared compare VERB admet-389 4 9 in in ADP admet-389 4 10 wild wild ADJ admet-389 4 11 - - PUNCT admet-389 4 12 type type NOUN admet-389 4 13 ( ( PUNCT admet-389 4 14 wt wt NOUN admet-389 4 15 ) ) PUNCT admet-389 4 16 and and CCONJ admet-389 4 17 human human ADJ admet-389 4 18 organic organic ADJ admet-389 4 19 cation cation NOUN admet-389 4 20 transporter transporter NOUN admet-389 4 21 2 2 NUM admet-389 4 22 ( ( PUNCT admet-389 4 23 hoct2)-stably hoct2)-stably ADV admet-389 4 24 transfected transfecte VERB admet-389 4 25 chinese chinese ADJ admet-389 4 26 hamster hamster NOUN admet-389 4 27 ovary ovary NOUN admet-389 4 28 ( ( PUNCT admet-389 4 29 cho cho ADJ admet-389 4 30 ) ) PUNCT admet-389 4 31 cells cell NOUN admet-389 4 32 . . PUNCT admet-389 5 1 all all DET admet-389 5 2 the the DET admet-389 5 3 compounds compound NOUN admet-389 5 4 were be AUX admet-389 5 5 accumulated accumulate VERB admet-389 5 6 by by ADP admet-389 5 7 the the DET admet-389 5 8 cho cho ADJ admet-389 5 9 - - PUNCT admet-389 5 10 hoct2 hoct2 NOUN admet-389 5 11 cells cell NOUN admet-389 5 12 . . PUNCT admet-389 6 1 intracellular intracellular ADJ admet-389 6 2 uptake uptake NOUN admet-389 6 3 of of ADP admet-389 6 4 the the DET admet-389 6 5 compounds compound NOUN admet-389 6 6 was be AUX admet-389 6 7 higher high ADJ admet-389 6 8 in in ADP admet-389 6 9 cho cho NOUN admet-389 6 10 cells cell NOUN admet-389 6 11 stably stably ADV admet-389 6 12 - - PUNCT admet-389 6 13 expressing express VERB admet-389 6 14 hoct2 hoct2 NOUN admet-389 6 15 compared compare VERB admet-389 6 16 to to ADP admet-389 6 17 the the DET admet-389 6 18 wt wt NOUN admet-389 6 19 . . PUNCT admet-389 7 1 the the DET admet-389 7 2 uptake uptake NOUN admet-389 7 3 was be AUX admet-389 7 4 concentration concentration NOUN admet-389 7 5 – – PUNCT admet-389 7 6 dependent dependent ADJ admet-389 7 7 and and CCONJ admet-389 7 8 saturable saturable ADJ admet-389 7 9 ( ( PUNCT admet-389 7 10 except except SCONJ admet-389 7 11 for for ADP admet-389 7 12 rhodamine rhodamine NOUN admet-389 7 13 123 123 NUM admet-389 7 14 ) ) PUNCT admet-389 7 15 . . PUNCT admet-389 8 1 the the DET admet-389 8 2 affinities affinity NOUN admet-389 8 3 of of ADP admet-389 8 4 the the DET admet-389 8 5 compounds compound NOUN admet-389 8 6 for for ADP admet-389 8 7 the the DET admet-389 8 8 hoct2 hoct2 NOUN admet-389 8 9 ( ( PUNCT admet-389 8 10 in in ADP admet-389 8 11 descending descend VERB admet-389 8 12 order order NOUN admet-389 8 13 ) ) PUNCT admet-389 8 14 were be AUX admet-389 8 15 : : PUNCT admet-389 8 16 amiloride amiloride ADV admet-389 8 17 ( ( PUNCT admet-389 8 18 km km NOUN admet-389 8 19 = = SYM admet-389 8 20 72.63 72.63 NUM admet-389 8 21 12.02 12.02 NUM admet-389 8 22 µm µm NOUN admet-389 8 23 ) ) PUNCT admet-389 8 24 > > X admet-389 9 1 rhodamine rhodamine NOUN admet-389 9 2 6 6 NUM admet-389 9 3 g g NOUN admet-389 9 4 ( ( PUNCT admet-389 9 5 km km NOUN admet-389 9 6 = = SYM admet-389 9 7 82.47 82.47 NUM admet-389 9 8   PROPN admet-389 9 9 29.15 29.15 NUM admet-389 9 10 µm µm NOUN admet-389 9 11 ) ) PUNCT admet-389 9 12 . . PUNCT admet-389 10 1 uptake uptake ADJ admet-389 10 2 of of ADP admet-389 10 3 amiloride amiloride NOUN admet-389 10 4 in in ADP admet-389 10 5 transfected transfecte VERB admet-389 10 6 cells cell NOUN admet-389 10 7 was be AUX admet-389 10 8 ph ph ADJ admet-389 10 9 -dependent -dependent ADJ admet-389 10 10 and and CCONJ admet-389 10 11 significantly significantly ADV admet-389 10 12 inhibited inhibit VERB admet-389 10 13 by by ADP admet-389 10 14 hoct2 hoct2 NOUN admet-389 10 15 inhibitors inhibitor NOUN admet-389 10 16 ( ( PUNCT admet-389 10 17 quinine quinine NOUN admet-389 10 18 , , PUNCT admet-389 10 19 verapamil verapamil NOUN admet-389 10 20 and and CCONJ admet-389 10 21 quinidine quinidine NOUN admet-389 10 22 ) ) PUNCT admet-389 10 23 . . PUNCT admet-389 11 1 based base VERB admet-389 11 2 on on ADP admet-389 11 3 our our PRON admet-389 11 4 kinetic kinetic ADJ admet-389 11 5 data datum NOUN admet-389 11 6 and and CCONJ admet-389 11 7 other other ADJ admet-389 11 8 considerations consideration NOUN admet-389 11 9 , , PUNCT admet-389 11 10 we we PRON admet-389 11 11 recommend recommend VERB admet-389 11 12 the the DET admet-389 11 13 use use NOUN admet-389 11 14 of of ADP admet-389 11 15 amiloride amiloride NOUN admet-389 11 16 for for ADP admet-389 11 17 characterizing characterize VERB admet-389 11 18 hoct2 hoct2 NOUN admet-389 11 19 transporters transporter NOUN admet-389 11 20 . . PUNCT admet-389 12 1 keywords keyword VERB admet-389 12 2 transporters transporter NOUN admet-389 12 3 ; ; PUNCT admet-389 12 4 oct2 oct2 NOUN admet-389 12 5 ; ; PUNCT admet-389 12 6 cho cho NOUN admet-389 12 7 cells cell NOUN admet-389 12 8 ; ; PUNCT admet-389 12 9 amiloride amiloride ADV admet-389 12 10 ; ; PUNCT admet-389 12 11 rhodamine rhodamine NOUN admet-389 12 12 ; ; PUNCT admet-389 12 13 fluorescence fluorescence NOUN admet-389 12 14 introduction introduction NOUN admet-389 12 15 organic organic ADJ admet-389 12 16 cations cation NOUN admet-389 12 17 are be AUX admet-389 12 18 positively positively ADV admet-389 12 19 charged charge VERB admet-389 12 20 amines amine NOUN admet-389 12 21 or or CCONJ admet-389 12 22 organic organic ADJ admet-389 12 23 molecules molecule NOUN admet-389 12 24 with with ADP admet-389 12 25 transient transient ADJ admet-389 12 26 or or CCONJ admet-389 12 27 permanent permanent ADJ admet-389 12 28 net net ADJ admet-389 12 29 positive positive ADJ admet-389 12 30 charge(s charge(s NOUN admet-389 12 31 ) ) PUNCT admet-389 12 32 at at ADP admet-389 12 33 physiological physiological ADJ admet-389 12 34 ph ph NOUN admet-389 13 1 [ [ X admet-389 13 2 1 1 NUM admet-389 13 3 ] ] PUNCT admet-389 13 4 . . PUNCT admet-389 14 1 a a DET admet-389 14 2 wide wide ADJ admet-389 14 3 variety variety NOUN admet-389 14 4 of of ADP admet-389 14 5 clinically clinically ADV admet-389 14 6 used use VERB admet-389 14 7 drugs drug NOUN admet-389 14 8 ( ( PUNCT admet-389 14 9 e.g. e.g. ADV admet-389 14 10 cimetidine cimetidine NOUN admet-389 14 11 , , PUNCT admet-389 14 12 procainamide procainamide ADJ admet-389 14 13 , , PUNCT admet-389 14 14 quinidine quinidine ADJ admet-389 14 15 , , PUNCT admet-389 14 16 salbutamol salbutamol NOUN admet-389 14 17 ) ) PUNCT admet-389 14 18 and and CCONJ admet-389 14 19 endogenous endogenous ADJ admet-389 14 20 bioactive bioactive ADJ admet-389 14 21 amines amine NOUN admet-389 14 22 ( ( PUNCT admet-389 14 23 e.g. e.g. ADV admet-389 14 24 dopamine dopamine NOUN admet-389 14 25 , , PUNCT admet-389 14 26 histamine histamine NOUN admet-389 14 27 , , PUNCT admet-389 14 28 nmethylnicotinamide nmethylnicotinamide NOUN admet-389 14 29 , , PUNCT admet-389 14 30 choline choline NOUN admet-389 14 31 ) ) PUNCT admet-389 14 32 are be AUX admet-389 14 33 organic organic ADJ admet-389 14 34 cations cation NOUN admet-389 14 35 [ [ X admet-389 14 36 1 1 NUM admet-389 14 37 - - SYM admet-389 14 38 2 2 NUM admet-389 14 39 ] ] PUNCT admet-389 14 40 . . PUNCT admet-389 15 1 these these DET admet-389 15 2 charged charge VERB admet-389 15 3 molecules molecule NOUN admet-389 15 4 are be AUX admet-389 15 5 transported transport VERB admet-389 15 6 across across ADP admet-389 15 7 cell cell NOUN admet-389 15 8 epithelia epithelium NOUN admet-389 15 9 by by ADP admet-389 15 10 carrier carrier NOUN admet-389 15 11 - - PUNCT admet-389 15 12 mediated mediate VERB admet-389 15 13 transport transport NOUN admet-389 15 14 proteins protein NOUN admet-389 15 15 , , PUNCT admet-389 15 16 mainly mainly ADV admet-389 15 17 organic organic ADJ admet-389 15 18 cation cation NOUN admet-389 15 19 transporters transporter NOUN admet-389 15 20 ( ( PUNCT admet-389 15 21 octs oct NOUN admet-389 15 22 ) ) PUNCT admet-389 15 23 and and CCONJ admet-389 15 24 proton proton NOUN admet-389 15 25 / / SYM admet-389 15 26 cation cation NOUN admet-389 15 27 antiporters antiporter NOUN admet-389 15 28 ( ( PUNCT admet-389 15 29 mates mate NOUN admet-389 15 30 ) ) PUNCT admet-389 16 1 [ [ X admet-389 16 2 3 3 NUM admet-389 16 3 - - SYM admet-389 16 4 6 6 NUM admet-389 16 5 ] ] PUNCT admet-389 16 6 . . PUNCT admet-389 17 1 these these DET admet-389 17 2 transporters transporter NOUN admet-389 17 3 play play VERB admet-389 17 4 pivotal pivotal ADJ admet-389 17 5 roles role NOUN admet-389 17 6 in in ADP admet-389 17 7 the the DET admet-389 17 8 absorption absorption NOUN admet-389 17 9 , , PUNCT admet-389 17 10 distribution distribution NOUN admet-389 17 11 and and CCONJ admet-389 17 12 disposition disposition NOUN admet-389 17 13 of of ADP admet-389 17 14 cationic cationic ADJ admet-389 17 15 drugs drug NOUN admet-389 17 16 in in ADP admet-389 17 17 various various ADJ admet-389 17 18 tissues tissue NOUN admet-389 17 19 . . PUNCT admet-389 18 1 the the DET admet-389 18 2 octs oct NOUN admet-389 18 3 are be AUX admet-389 18 4 members member NOUN admet-389 18 5 of of ADP admet-389 18 6 the the DET admet-389 18 7 solute solute NOUN admet-389 18 8 carrier carrier NOUN admet-389 18 9 family family NOUN admet-389 18 10 , , PUNCT admet-389 18 11 slc22a slc22a PRON admet-389 18 12 [ [ X admet-389 18 13 3 3 NUM admet-389 18 14 - - SYM admet-389 18 15 4 4 NUM admet-389 18 16 , , PUNCT admet-389 18 17 7 7 NUM admet-389 18 18 ] ] PUNCT admet-389 18 19 . . PUNCT admet-389 19 1 the the DET admet-389 19 2 slc22 slc22 NOUN admet-389 19 3 family family NOUN admet-389 19 4 is be AUX admet-389 19 5 sub sub ADJ admet-389 19 6 - - ADJ admet-389 19 7 grouped group VERB admet-389 19 8 according accord VERB admet-389 19 9 to to ADP admet-389 19 10 substrates substrate NOUN admet-389 19 11 and and CCONJ admet-389 19 12 based base VERB admet-389 19 13 on on ADP admet-389 19 14 the the DET admet-389 19 15 driving drive VERB admet-389 19 16 force force NOUN admet-389 19 17 for for ADP admet-389 19 18 the the DET admet-389 19 19 cation cation NOUN admet-389 19 20 transport transport NOUN admet-389 19 21 mechanisms mechanism NOUN admet-389 19 22 [ [ X admet-389 19 23 1 1 NUM admet-389 19 24 , , PUNCT admet-389 19 25 3 3 NUM admet-389 19 26 - - SYM admet-389 19 27 4 4 NUM admet-389 19 28 ] ] PUNCT admet-389 19 29 . . PUNCT admet-389 20 1 one one NUM admet-389 20 2 of of ADP admet-389 20 3 such such ADJ admet-389 20 4 subgroups subgroup NOUN admet-389 20 5 , , PUNCT admet-389 20 6 which which PRON admet-389 20 7 translocate translocate VERB admet-389 20 8 organic organic ADJ admet-389 20 9 http://www.pub.iapchem.org/ojs/index.php/admet/index http://www.pub.iapchem.org/ojs/index.php/admet/index NOUN admet-389 20 10 mailto:remigius.agu@dal.cab mailto:remigius.agu@dal.cab NOUN admet-389 20 11 remigius remigius PROPN admet-389 20 12 agu agu PROPN admet-389 20 13 et et PROPN admet-389 20 14 al al PROPN admet-389 20 15 . . PROPN admet-389 20 16 admet admet PROPN admet-389 20 17 & & CCONJ admet-389 20 18 dmpk dmpk PROPN admet-389 20 19 5(2 5(2 NUM admet-389 20 20 ) ) PUNCT admet-389 20 21 ( ( PUNCT admet-389 20 22 2017 2017 NUM admet-389 20 23 ) ) PUNCT admet-389 20 24 135 135 NUM admet-389 20 25 - - SYM admet-389 20 26 145 145 NUM admet-389 20 27 136 136 NUM admet-389 20 28 cations cation NOUN admet-389 20 29 and and CCONJ admet-389 20 30 weak weak ADJ admet-389 20 31 bases basis NOUN admet-389 20 32 in in ADP admet-389 20 33 an an DET admet-389 20 34 electrogenic- electrogenic- NOUN admet-389 20 35 , , PUNCT admet-389 20 36 sodium sodium NOUN admet-389 20 37 chlorideand chlorideand NOUN admet-389 20 38 proton proton NOUN admet-389 20 39 gradient gradient NOUN admet-389 20 40 - - PUNCT admet-389 20 41 independent independent ADJ admet-389 20 42 manner manner NOUN admet-389 20 43 , , PUNCT admet-389 20 44 is be AUX admet-389 20 45 the the DET admet-389 20 46 slc22a1 slc22a1 NOUN admet-389 20 47 - - PUNCT admet-389 20 48 3 3 NUM admet-389 20 49 ( ( PUNCT admet-389 20 50 oct1 oct1 NOUN admet-389 20 51 - - PUNCT admet-389 20 52 3 3 NUM admet-389 20 53 ) ) PUNCT admet-389 20 54 . . PUNCT admet-389 21 1 these these DET admet-389 21 2 facilitative facilitative ADJ admet-389 21 3 diffusion diffusion NOUN admet-389 21 4 systems system NOUN admet-389 21 5 transport transport VERB admet-389 21 6 organic organic ADJ admet-389 21 7 cations cation NOUN admet-389 21 8 in in ADP admet-389 21 9 both both DET admet-389 21 10 directions direction NOUN admet-389 21 11 across across ADP admet-389 21 12 the the DET admet-389 21 13 plasma plasma NOUN admet-389 21 14 membrane membrane NOUN admet-389 21 15 . . PUNCT admet-389 22 1 other other ADJ admet-389 22 2 slc22 slc22 NOUN admet-389 22 3 transporter transporter NOUN admet-389 22 4 subgroups subgroup NOUN admet-389 22 5 are be AUX admet-389 22 6 the the DET admet-389 22 7 sodiumand sodiumand ADJ admet-389 22 8 ph ph ADJ admet-389 22 9 - - ADJ admet-389 22 10 dependent dependent ADJ admet-389 22 11 cation cation NOUN admet-389 22 12 / / SYM admet-389 22 13 carnitine carnitine NOUN admet-389 22 14 transporters transporter NOUN admet-389 22 15 , , PUNCT admet-389 22 16 slc22a4 slc22a4 PROPN admet-389 22 17 ( ( PUNCT admet-389 22 18 octn1 octn1 ADJ admet-389 22 19 ) ) PUNCT admet-389 22 20 and and CCONJ admet-389 22 21 slc22a5 slc22a5 PROPN admet-389 22 22 ( ( PUNCT admet-389 22 23 octn2 octn2 ADV admet-389 22 24 ) ) PUNCT admet-389 23 1 [ [ X admet-389 23 2 8 8 NUM admet-389 23 3 - - SYM admet-389 23 4 10 10 NUM admet-389 23 5 ] ] PUNCT admet-389 23 6 . . PUNCT admet-389 24 1 functionally functionally ADV admet-389 24 2 , , PUNCT admet-389 24 3 the the DET admet-389 24 4 octs oct NOUN admet-389 24 5 and and CCONJ admet-389 24 6 octns octns NOUN admet-389 24 7 play play VERB admet-389 24 8 a a DET admet-389 24 9 crucial crucial ADJ admet-389 24 10 role role NOUN admet-389 24 11 in in ADP admet-389 24 12 the the DET admet-389 24 13 regulation regulation NOUN admet-389 24 14 of of ADP admet-389 24 15 cellular cellular ADJ admet-389 24 16 functions function NOUN admet-389 24 17 . . PUNCT admet-389 25 1 their their PRON admet-389 25 2 involvement involvement NOUN admet-389 25 3 in in ADP admet-389 25 4 cationic cationic ADJ admet-389 25 5 solute solute NOUN admet-389 25 6 absorption absorption NOUN admet-389 25 7 , , PUNCT admet-389 25 8 transport transport NOUN admet-389 25 9 and and CCONJ admet-389 25 10 excretion excretion NOUN admet-389 25 11 in in ADP admet-389 25 12 the the DET admet-389 25 13 intestinal intestinal ADJ admet-389 25 14 , , PUNCT admet-389 25 15 placental placental NOUN admet-389 25 16 , , PUNCT admet-389 25 17 nasal nasal NOUN admet-389 25 18 and and CCONJ admet-389 25 19 renal renal ADJ admet-389 25 20 epithelia epithelia PROPN admet-389 25 21 is be AUX admet-389 25 22 well well ADV admet-389 25 23 documented document VERB admet-389 25 24 [ [ PUNCT admet-389 25 25 10 10 NUM admet-389 25 26 - - SYM admet-389 25 27 11 11 NUM admet-389 25 28 ] ] PUNCT admet-389 25 29 . . PUNCT admet-389 26 1 among among ADP admet-389 26 2 the the DET admet-389 26 3 organic organic ADJ admet-389 26 4 cation cation NOUN admet-389 26 5 transporters transporter NOUN admet-389 26 6 , , PUNCT admet-389 26 7 the the DET admet-389 26 8 oct2 oct2 NOUN admet-389 26 9 is be AUX admet-389 26 10 the the DET admet-389 26 11 transporter transporter NOUN admet-389 26 12 with with ADP admet-389 26 13 the the DET admet-389 26 14 potential potential NOUN admet-389 26 15 to to PART admet-389 26 16 significantly significantly ADV admet-389 26 17 alter alter VERB admet-389 26 18 drug drug NOUN admet-389 26 19 disposition disposition NOUN admet-389 26 20 . . PUNCT admet-389 27 1 based base VERB admet-389 27 2 on on ADP admet-389 27 3 this this DET admet-389 27 4 fact fact NOUN admet-389 27 5 , , PUNCT admet-389 27 6 the the DET admet-389 27 7 us us PROPN admet-389 27 8 food food NOUN admet-389 27 9 and and CCONJ admet-389 27 10 drugs drugs PROPN admet-389 27 11 administration administration PROPN admet-389 27 12 agency agency PROPN admet-389 27 13 ( ( PUNCT admet-389 27 14 fda fda PROPN admet-389 27 15 ) ) PUNCT admet-389 27 16 and and CCONJ admet-389 27 17 european european ADJ admet-389 27 18 medicines medicine NOUN admet-389 27 19 agency agency NOUN admet-389 27 20 ( ( PUNCT admet-389 27 21 ema ema PROPN admet-389 27 22 ) ) PUNCT admet-389 27 23 require require VERB admet-389 27 24 that that SCONJ admet-389 27 25 new new ADJ admet-389 27 26 drug drug NOUN admet-389 27 27 candidates candidate NOUN admet-389 27 28 be be AUX admet-389 27 29 screened screen VERB admet-389 27 30 for for ADP admet-389 27 31 oct2 oct2 NOUN admet-389 27 32 activity activity NOUN admet-389 27 33 during during ADP admet-389 27 34 early early ADJ admet-389 27 35 stages stage NOUN admet-389 27 36 of of ADP admet-389 27 37 drug drug NOUN admet-389 27 38 development development NOUN admet-389 27 39 if if SCONJ admet-389 27 40 active active ADJ admet-389 27 41 renal renal ADJ admet-389 27 42 excretion excretion NOUN admet-389 27 43 is be AUX admet-389 27 44 envisaged envisage VERB admet-389 28 1 [ [ PUNCT admet-389 28 2 12 12 NUM admet-389 28 3 - - SYM admet-389 28 4 13 13 NUM admet-389 28 5 ] ] PUNCT admet-389 28 6 . . PUNCT admet-389 29 1 the the DET admet-389 29 2 transporter transporter NOUN admet-389 29 3 is be AUX admet-389 29 4 mainly mainly ADV admet-389 29 5 expressed express VERB admet-389 29 6 in in ADP admet-389 29 7 the the DET admet-389 29 8 basolateral basolateral ADJ admet-389 29 9 membrane membrane NOUN admet-389 29 10 of of ADP admet-389 29 11 renal renal ADJ admet-389 29 12 proximal proximal ADJ admet-389 29 13 tubules tubule NOUN admet-389 29 14 and and CCONJ admet-389 29 15 is be AUX admet-389 29 16 also also ADV admet-389 29 17 expressed express VERB admet-389 29 18 in in ADP admet-389 29 19 small small ADJ admet-389 29 20 intestine intestine NOUN admet-389 29 21 , , PUNCT admet-389 29 22 lung lung NOUN admet-389 29 23 , , PUNCT admet-389 29 24 placenta placenta ADJ admet-389 29 25 , , PUNCT admet-389 29 26 thymus thymus VERB admet-389 29 27 , , PUNCT admet-389 29 28 brain brain NOUN admet-389 29 29 and and CCONJ admet-389 29 30 the the DET admet-389 29 31 inner inner ADJ admet-389 29 32 ear ear NOUN admet-389 30 1 [ [ X admet-389 30 2 2 2 NUM admet-389 30 3 , , PUNCT admet-389 30 4 14 14 NUM admet-389 30 5 - - SYM admet-389 30 6 15 15 NUM admet-389 30 7 ] ] PUNCT admet-389 30 8 . . PUNCT admet-389 31 1 although although SCONJ admet-389 31 2 the the DET admet-389 31 3 role role NOUN admet-389 31 4 of of ADP admet-389 31 5 the the DET admet-389 31 6 transporter transporter NOUN admet-389 31 7 in in ADP admet-389 31 8 other other ADJ admet-389 31 9 tissues tissue NOUN admet-389 31 10 than than ADP admet-389 31 11 the the DET admet-389 31 12 kidney kidney NOUN admet-389 31 13 is be AUX admet-389 31 14 still still ADV admet-389 31 15 under under ADP admet-389 31 16 investigation investigation NOUN admet-389 31 17 , , PUNCT admet-389 31 18 the the DET admet-389 31 19 oct2 oct2 NOUN admet-389 31 20 is be AUX admet-389 31 21 critical critical ADJ admet-389 31 22 for for ADP admet-389 31 23 the the DET admet-389 31 24 detoxification detoxification NOUN admet-389 31 25 and and CCONJ admet-389 31 26 elimination elimination NOUN admet-389 31 27 of of ADP admet-389 31 28 drugs drug NOUN admet-389 31 29 and and CCONJ admet-389 31 30 endogenous endogenous ADJ admet-389 31 31 compounds compound NOUN admet-389 31 32 from from ADP admet-389 31 33 the the DET admet-389 31 34 systemic systemic ADJ admet-389 31 35 circulation circulation NOUN admet-389 31 36 and and CCONJ admet-389 31 37 thus thus ADV admet-389 31 38 a a DET admet-389 31 39 significant significant ADJ admet-389 31 40 determinant determinant NOUN admet-389 31 41 of of ADP admet-389 31 42 drug drug NOUN admet-389 31 43 response response NOUN admet-389 31 44 and and CCONJ admet-389 31 45 sensitivity sensitivity NOUN admet-389 31 46 [ [ X admet-389 31 47 2 2 NUM admet-389 31 48 , , PUNCT admet-389 31 49 4 4 NUM admet-389 31 50 ] ] PUNCT admet-389 31 51 . . PUNCT admet-389 32 1 the the DET admet-389 32 2 most most ADV admet-389 32 3 commonly commonly ADV admet-389 32 4 used use VERB admet-389 32 5 method method NOUN admet-389 32 6 for for ADP admet-389 32 7 membrane membrane NOUN admet-389 32 8 transporter transporter NOUN admet-389 32 9 characterization characterization NOUN admet-389 32 10 is be AUX admet-389 32 11 radioactivity radioactivity NOUN admet-389 32 12 . . PUNCT admet-389 33 1 however however ADV admet-389 33 2 , , PUNCT admet-389 33 3 this this DET admet-389 33 4 approach approach NOUN admet-389 33 5 is be AUX admet-389 33 6 neither neither CCONJ admet-389 33 7 convenient convenient ADJ admet-389 33 8 nor nor CCONJ admet-389 33 9 cheap cheap ADJ admet-389 33 10 due due ADJ admet-389 33 11 isotope isotope NOUN admet-389 33 12 handling handling NOUN admet-389 33 13 , , PUNCT admet-389 33 14 disposal disposal NOUN admet-389 33 15 measures measure NOUN admet-389 33 16 and and CCONJ admet-389 33 17 regulatory regulatory ADJ admet-389 33 18 restrictions restriction NOUN admet-389 33 19 . . PUNCT admet-389 34 1 furthermore furthermore ADV admet-389 34 2 , , PUNCT admet-389 34 3 there there PRON admet-389 34 4 is be VERB admet-389 34 5 potential potential ADJ admet-389 34 6 health health NOUN admet-389 34 7 risk risk NOUN admet-389 34 8 associated associate VERB admet-389 34 9 with with ADP admet-389 34 10 the the DET admet-389 34 11 use use NOUN admet-389 34 12 of of ADP admet-389 34 13 radiolabelled radiolabelle VERB admet-389 34 14 substrates substrate NOUN admet-389 34 15 if if SCONJ admet-389 34 16 handled handle VERB admet-389 34 17 incorrectly incorrectly ADV admet-389 34 18 . . PUNCT admet-389 35 1 currently currently ADV admet-389 35 2 , , PUNCT admet-389 35 3 there there PRON admet-389 35 4 is be VERB admet-389 35 5 a a DET admet-389 35 6 growing grow VERB admet-389 35 7 interest interest NOUN admet-389 35 8 in in ADP admet-389 35 9 the the DET admet-389 35 10 development development NOUN admet-389 35 11 of of ADP admet-389 35 12 low low ADJ admet-389 35 13 - - PUNCT admet-389 35 14 risk risk NOUN admet-389 35 15 screening screening NOUN admet-389 35 16 technologies technology NOUN admet-389 35 17 for for ADP admet-389 35 18 drug drug NOUN admet-389 35 19 transporters transporter NOUN admet-389 35 20 characterization characterization NOUN admet-389 35 21 [ [ X admet-389 35 22 16 16 NUM admet-389 35 23 - - SYM admet-389 35 24 17 17 NUM admet-389 35 25 ] ] PUNCT admet-389 35 26 . . PUNCT admet-389 36 1 unlike unlike ADP admet-389 36 2 radioactivity radioactivity NOUN admet-389 36 3 , , PUNCT admet-389 36 4 fluorescent fluorescent ADJ admet-389 36 5 assays assay NOUN admet-389 36 6 significantly significantly ADV admet-389 36 7 improve improve VERB admet-389 36 8 laboratory laboratory NOUN admet-389 36 9 safety safety NOUN admet-389 36 10 with with ADP admet-389 36 11 the the DET admet-389 36 12 opportunity opportunity NOUN admet-389 36 13 for for ADP admet-389 36 14 real real ADJ admet-389 36 15 - - PUNCT admet-389 36 16 time time NOUN admet-389 36 17 monitoring monitoring NOUN admet-389 36 18 using use VERB admet-389 36 19 fluorimeters fluorimeter NOUN admet-389 36 20 . . PUNCT admet-389 37 1 currently currently ADV admet-389 37 2 , , PUNCT admet-389 37 3 4 4 NUM admet-389 37 4 - - PUNCT admet-389 37 5 di-1 di-1 PRON admet-389 37 6 - - PUNCT admet-389 37 7 asp asp NOUN admet-389 37 8 is be AUX admet-389 37 9 the the DET admet-389 37 10 main main ADJ admet-389 37 11 fluorescent fluorescent NOUN admet-389 37 12 probe probe NOUN admet-389 37 13 for for ADP admet-389 37 14 screening screen VERB admet-389 37 15 organic organic ADJ admet-389 37 16 cation cation NOUN admet-389 37 17 transporters transporter NOUN admet-389 37 18 . . PUNCT admet-389 38 1 it it PRON admet-389 38 2 has have VERB admet-389 38 3 a a DET admet-389 38 4 permanent permanent ADJ admet-389 38 5 positive positive ADJ admet-389 38 6 charge charge NOUN admet-389 38 7 , , PUNCT admet-389 38 8 high high ADJ admet-389 38 9 fluorescent fluorescent ADJ admet-389 38 10 intensity intensity NOUN admet-389 38 11 and and CCONJ admet-389 38 12 stable stable ADJ admet-389 38 13 over over ADP admet-389 38 14 a a DET admet-389 38 15 range range NOUN admet-389 38 16 of of ADP admet-389 38 17 ph ph NOUN admet-389 38 18 and and CCONJ admet-389 38 19 temperature temperature NOUN admet-389 38 20 . . PUNCT admet-389 39 1 however however ADV admet-389 39 2 , , PUNCT admet-389 39 3 the the DET admet-389 39 4 compound compound NOUN admet-389 39 5 is be AUX admet-389 39 6 transported transport VERB admet-389 39 7 by by ADP admet-389 39 8 all all DET admet-389 39 9 oct oct NOUN admet-389 39 10 isoforms isoform NOUN admet-389 39 11 ( ( PUNCT admet-389 39 12 oct1 oct1 NOUN admet-389 39 13 - - PUNCT admet-389 39 14 2 2 NUM admet-389 39 15 , , PUNCT admet-389 39 16 octn1 octn1 ADJ admet-389 39 17 and and CCONJ admet-389 39 18 octn2 octn2 ADJ admet-389 39 19 ) ) PUNCT admet-389 39 20 . . PUNCT admet-389 40 1 it it PRON admet-389 40 2 is be AUX admet-389 40 3 not not PART admet-389 40 4 among among ADP admet-389 40 5 the the DET admet-389 40 6 compounds compound NOUN admet-389 40 7 recommended recommend VERB admet-389 40 8 by by ADP admet-389 40 9 the the DET admet-389 40 10 international international ADJ admet-389 40 11 transporter transporter NOUN admet-389 40 12 consortium consortium NOUN admet-389 40 13 for for ADP admet-389 40 14 investigating investigate VERB admet-389 40 15 oct2 oct2 NOUN admet-389 40 16 [ [ X admet-389 40 17 18 18 NUM admet-389 40 18 ] ] PUNCT admet-389 40 19 . . PUNCT admet-389 41 1 it it PRON admet-389 41 2 is be AUX admet-389 41 3 therefore therefore ADV admet-389 41 4 relevant relevant ADJ admet-389 41 5 to to PART admet-389 41 6 explore explore VERB admet-389 41 7 the the DET admet-389 41 8 use use NOUN admet-389 41 9 of of ADP admet-389 41 10 other other ADJ admet-389 41 11 fluorescent fluorescent ADJ admet-389 41 12 organic organic ADJ admet-389 41 13 cation cation NOUN admet-389 41 14 probes probe NOUN admet-389 41 15 that that PRON admet-389 41 16 may may AUX admet-389 41 17 specifically specifically ADV admet-389 41 18 interact interact VERB admet-389 41 19 with with ADP admet-389 41 20 oct2 oct2 NOUN admet-389 41 21 . . PUNCT admet-389 42 1 the the DET admet-389 42 2 objective objective NOUN admet-389 42 3 of of ADP admet-389 42 4 this this DET admet-389 42 5 study study NOUN admet-389 42 6 was be AUX admet-389 42 7 to to PART admet-389 42 8 investigate investigate VERB admet-389 42 9 the the DET admet-389 42 10 potential potential ADJ admet-389 42 11 use use NOUN admet-389 42 12 of of ADP admet-389 42 13 amiloride amiloride NOUN admet-389 42 14 , , PUNCT admet-389 42 15 rhodamine rhodamine NOUN admet-389 42 16 123 123 NUM admet-389 42 17 and and CCONJ admet-389 42 18 rhodamine rhodamine ADJ admet-389 42 19 6 6 NUM admet-389 42 20 g g NOUN admet-389 42 21 as as ADP admet-389 42 22 specific specific ADJ admet-389 42 23 fluorescent fluorescent NOUN admet-389 42 24 substrates substrate NOUN admet-389 42 25 for for ADP admet-389 42 26 hoct2 hoct2 NOUN admet-389 42 27 using use VERB admet-389 42 28 cho cho NOUN admet-389 42 29 cells cell NOUN admet-389 42 30 stably stably ADV admet-389 42 31 expressing express VERB admet-389 42 32 the the DET admet-389 42 33 transporter transporter NOUN admet-389 42 34 . . PUNCT admet-389 43 1 to to ADP admet-389 43 2 the the DET admet-389 43 3 best good ADJ admet-389 43 4 of of ADP admet-389 43 5 our our PRON admet-389 43 6 knowledge knowledge NOUN admet-389 43 7 , , PUNCT admet-389 43 8 there there PRON admet-389 43 9 is be VERB admet-389 43 10 no no DET admet-389 43 11 published publish VERB admet-389 43 12 study study NOUN admet-389 43 13 on on ADP admet-389 43 14 potential potential ADJ admet-389 43 15 interaction interaction NOUN admet-389 43 16 between between ADP admet-389 43 17 hoct2 hoct2 NOUN admet-389 43 18 stably stably ADV admet-389 43 19 expressed express VERB admet-389 43 20 in in ADP admet-389 43 21 cho cho NOUN admet-389 43 22 cells cell NOUN admet-389 43 23 and and CCONJ admet-389 43 24 the the DET admet-389 43 25 fluorescent fluorescent NOUN admet-389 43 26 substrates substrate NOUN admet-389 43 27 described describe VERB admet-389 43 28 in in ADP admet-389 43 29 this this DET admet-389 43 30 study study NOUN admet-389 43 31 . . PUNCT admet-389 44 1 experimental experimental ADJ admet-389 44 2 chemicals chemical NOUN admet-389 44 3 recovery recovery NOUN admet-389 44 4 ™ ™ ADJ admet-389 44 5 cell cell NOUN admet-389 44 6 culture culture NOUN admet-389 44 7 freezing freeze VERB admet-389 44 8 medium medium ADJ admet-389 44 9 , , PUNCT admet-389 44 10 kaighns kaighns NOUN admet-389 44 11 f12 f12 NOUN admet-389 44 12 , , PUNCT admet-389 44 13 0.25 0.25 NUM admet-389 44 14 % % NOUN admet-389 44 15 trypsin trypsin NOUN admet-389 44 16 - - PUNCT admet-389 44 17 edta edta NOUN admet-389 44 18 , , PUNCT admet-389 44 19 phenol phenol NOUN admet-389 44 20 red red NOUN admet-389 44 21 , , PUNCT admet-389 44 22 zeocin zeocin PRON admet-389 44 23 ™ ™ VERB admet-389 44 24 selection selection NOUN admet-389 44 25 reagent reagent NOUN admet-389 44 26 , , PUNCT admet-389 44 27 hygromycin hygromycin PROPN admet-389 44 28 b b PROPN admet-389 44 29 , , PUNCT admet-389 44 30 dmem dmem ADJ admet-389 44 31 - - PUNCT admet-389 44 32 f12 f12 NOUN admet-389 44 33 , , PUNCT admet-389 44 34 salmon salmon NOUN admet-389 44 35 sperm sperm PROPN admet-389 44 36 dna dna PROPN admet-389 44 37 , , PUNCT admet-389 44 38 fetal fetal ADJ admet-389 44 39 bovine bovine NOUN admet-389 44 40 serum serum NOUN admet-389 44 41 and and CCONJ admet-389 44 42 phosphatebuffered phosphatebuffere VERB admet-389 44 43 - - PUNCT admet-389 44 44 saline saline NOUN admet-389 44 45 ( ( PUNCT admet-389 44 46 pbs pbs PROPN admet-389 44 47 ) ) PUNCT admet-389 44 48 were be AUX admet-389 44 49 supplied supply VERB admet-389 44 50 by by ADP admet-389 44 51 life life NOUN admet-389 44 52 technologies technology NOUN admet-389 44 53 ( ( PUNCT admet-389 44 54 burlington burlington PROPN admet-389 44 55 , , PUNCT admet-389 44 56 on on ADP admet-389 44 57 canada canada PROPN admet-389 44 58 ) ) PUNCT admet-389 44 59 . . PUNCT admet-389 45 1 triton triton PROPN admet-389 45 2 x-100(r x-100(r PROPN admet-389 45 3 ) ) PUNCT admet-389 45 4 , , PUNCT admet-389 45 5 verapamil verapamil NOUN admet-389 45 6 , , PUNCT admet-389 45 7 quinine quinine NOUN admet-389 45 8 , , PUNCT admet-389 45 9 quinidine quinidine ADJ admet-389 45 10 , , PUNCT admet-389 45 11 corticosterone corticosterone NOUN admet-389 45 12 , , PUNCT admet-389 45 13 bovine bovine NOUN admet-389 45 14 serum serum NOUN admet-389 45 15 albumin albumin PROPN admet-389 45 16 ( ( PUNCT admet-389 45 17 bsa bsa PROPN admet-389 45 18 ) ) PUNCT admet-389 45 19 , , PUNCT admet-389 45 20 isopropanol isopropanol NOUN admet-389 45 21 , , PUNCT admet-389 45 22 hanks hanks PROPN admet-389 45 23 ’ ' PUNCT admet-389 45 24 balanced balanced ADJ admet-389 45 25 salt salt NOUN admet-389 45 26 ( ( PUNCT admet-389 45 27 hbss hbss NOUN admet-389 45 28 ) ) PUNCT admet-389 45 29 , , PUNCT admet-389 45 30 and and CCONJ admet-389 45 31 penicillin penicillin PROPN admet-389 45 32 / / SYM admet-389 45 33 streptomycin streptomycin PROPN admet-389 45 34 were be AUX admet-389 45 35 purchased purchase VERB admet-389 45 36 from from ADP admet-389 45 37 sigma sigma PROPN admet-389 45 38 ( ( PUNCT admet-389 45 39 st st PROPN admet-389 45 40 . . PROPN admet-389 45 41 louis louis PROPN admet-389 45 42 , , PUNCT admet-389 45 43 mo mo PROPN admet-389 45 44 , , PUNCT admet-389 45 45 usa usa PROPN admet-389 45 46 ) ) PUNCT admet-389 45 47 . . PUNCT admet-389 46 1 rhodamine rhodamine NOUN admet-389 46 2 123 123 NUM admet-389 46 3 , , PUNCT admet-389 46 4 rhodamine rhodamine NOUN admet-389 46 5 6 6 NUM admet-389 46 6 g g NOUN admet-389 46 7 and and CCONJ admet-389 46 8 amiloride amiloride NOUN admet-389 46 9 were be AUX admet-389 46 10 procured procure VERB admet-389 46 11 from from ADP admet-389 46 12 santa santa PROPN admet-389 46 13 cruz cruz PROPN admet-389 46 14 biotech biotech PROPN admet-389 46 15 inc inc PROPN admet-389 46 16 . . PROPN admet-389 46 17 ( ( PUNCT admet-389 46 18 dallas dallas PROPN admet-389 46 19 , , PUNCT admet-389 46 20 texas texas PROPN admet-389 46 21 , , PUNCT admet-389 46 22 usa usa PROPN admet-389 46 23 ) ) PUNCT admet-389 46 24 . . PUNCT admet-389 47 1 bicinchoninic bicinchoninic ADJ admet-389 47 2 acid acid PROPN admet-389 47 3 ( ( PUNCT admet-389 47 4 bca bca PROPN admet-389 47 5 ) ) PUNCT admet-389 47 6 protein protein NOUN admet-389 47 7 assay assay NOUN admet-389 47 8 kit kit NOUN admet-389 47 9 was be AUX admet-389 47 10 from from ADP admet-389 47 11 millipore millipore PROPN admet-389 47 12 ( ( PUNCT admet-389 47 13 billerica billerica PROPN admet-389 47 14 , , PUNCT admet-389 47 15 ma ma PROPN admet-389 47 16 , , PUNCT admet-389 47 17 usa usa PROPN admet-389 47 18 ) ) PUNCT admet-389 47 19 for for ADP admet-389 47 20 protein protein NOUN admet-389 47 21 assay assay NOUN admet-389 47 22 according accord VERB admet-389 47 23 to to ADP admet-389 47 24 the the DET admet-389 47 25 method method NOUN admet-389 47 26 described describe VERB admet-389 47 27 by by ADP admet-389 47 28 smith smith PROPN admet-389 47 29 et et PROPN admet-389 47 30 al al PROPN admet-389 48 1 [ [ X admet-389 48 2 19 19 NUM admet-389 48 3 ] ] PUNCT admet-389 48 4 . . PUNCT admet-389 49 1 admet admet PROPN admet-389 49 2 & & CCONJ admet-389 49 3 dmpk dmpk PROPN admet-389 49 4 5(2 5(2 NUM admet-389 49 5 ) ) PUNCT admet-389 49 6 ( ( PUNCT admet-389 49 7 2017 2017 NUM admet-389 49 8 ) ) PUNCT admet-389 49 9 135 135 NUM admet-389 49 10 - - SYM admet-389 49 11 145 145 NUM admet-389 49 12 fluorescent fluorescent ADJ admet-389 49 13 organic organic ADJ admet-389 49 14 cations cation NOUN admet-389 49 15 for for ADP admet-389 49 16 human human ADJ admet-389 49 17 oct2 oct2 NOUN admet-389 49 18 transporters transporter NOUN admet-389 49 19 screening screen VERB admet-389 49 20 doi doi NOUN admet-389 49 21 : : PUNCT admet-389 49 22 10.5599 10.5599 NUM admet-389 49 23 / / SYM admet-389 49 24 admet.5.2.389 admet.5.2.389 NOUN admet-389 49 25 137 137 NUM admet-389 49 26 chinese chinese ADJ admet-389 49 27 hamster hamster NOUN admet-389 49 28 ovary ovary NOUN admet-389 49 29 ( ( PUNCT admet-389 49 30 cho cho NOUN admet-389 49 31 ) ) PUNCT admet-389 49 32 cell cell NOUN admet-389 49 33 culture culture NOUN admet-389 49 34 and and CCONJ admet-389 49 35 stable stable ADJ admet-389 49 36 expression expression NOUN admet-389 49 37 of of ADP admet-389 49 38 hoct2 hoct2 PROPN admet-389 49 39 cho cho PROPN admet-389 49 40 cell cell NOUN admet-389 49 41 was be AUX admet-389 49 42 chosen choose VERB admet-389 49 43 for for ADP admet-389 49 44 stable stable ADJ admet-389 49 45 expression expression NOUN admet-389 49 46 of of ADP admet-389 49 47 hoct2 hoct2 NOUN admet-389 49 48 as as SCONJ admet-389 49 49 it it PRON admet-389 49 50 is be AUX admet-389 49 51 one one NUM admet-389 49 52 of of ADP admet-389 49 53 the the DET admet-389 49 54 models model NOUN admet-389 49 55 / / SYM admet-389 49 56 cultured cultured ADJ admet-389 49 57 cell cell NOUN admet-389 49 58 lines line NOUN admet-389 49 59 recommended recommend VERB admet-389 49 60 by by ADP admet-389 49 61 the the DET admet-389 49 62 international international ADJ admet-389 49 63 transporter transporter NOUN admet-389 49 64 consortium consortium NOUN admet-389 49 65 for for ADP admet-389 49 66 studying study VERB admet-389 49 67 drug drug NOUN admet-389 49 68 transporter transporter NOUN admet-389 49 69 interactions interaction NOUN admet-389 49 70 so so SCONJ admet-389 49 71 as as SCONJ admet-389 49 72 to to PART admet-389 49 73 assess assess VERB admet-389 49 74 uptake uptake NOUN admet-389 49 75 and and CCONJ admet-389 49 76 or or CCONJ admet-389 49 77 efflux efflux VERB admet-389 49 78 by by ADP admet-389 49 79 single single ADJ admet-389 49 80 recombinant recombinant ADJ admet-389 49 81 transporters transporter NOUN admet-389 50 1 [ [ X admet-389 50 2 18 18 NUM admet-389 50 3 ] ] X admet-389 50 4 ; ; PUNCT admet-389 50 5 and and CCONJ admet-389 50 6 there there PRON admet-389 50 7 is be VERB admet-389 50 8 no no DET admet-389 50 9 published publish VERB admet-389 50 10 study study NOUN admet-389 50 11 on on ADP admet-389 50 12 potential potential ADJ admet-389 50 13 interaction interaction NOUN admet-389 50 14 between between ADP admet-389 50 15 hoct2 hoct2 NOUN admet-389 50 16 stably stably ADV admet-389 50 17 expressed express VERB admet-389 50 18 in in ADP admet-389 50 19 cho cho NOUN admet-389 50 20 cells cell NOUN admet-389 50 21 and and CCONJ admet-389 50 22 the the DET admet-389 50 23 fluorescent fluorescent NOUN admet-389 50 24 substrates substrate NOUN admet-389 50 25 .the .the VERB admet-389 51 1 cho cho PROPN admet-389 51 2 cells cell NOUN admet-389 51 3 used use VERB admet-389 51 4 were be AUX admet-389 51 5 from from ADP admet-389 51 6 atcc atcc PROPN admet-389 51 7 ( ( PUNCT admet-389 51 8 manassas manassas PROPN admet-389 51 9 , , PUNCT admet-389 51 10 va va PROPN admet-389 51 11 , , PUNCT admet-389 51 12 usa usa PROPN admet-389 51 13 ) ) PUNCT admet-389 51 14 . . PUNCT admet-389 52 1 for for ADP admet-389 52 2 stable stable ADJ admet-389 52 3 expression expression NOUN admet-389 52 4 of of ADP admet-389 52 5 hoct2 hoct2 PROPN admet-389 52 6 , , PUNCT admet-389 52 7 cells cell NOUN admet-389 52 8 were be AUX admet-389 52 9 electroporated electroporate VERB admet-389 52 10 with with ADP admet-389 52 11 10 10 NUM admet-389 52 12 µg µg NOUN admet-389 52 13 of of ADP admet-389 52 14 hoct2 hoct2 NOUN admet-389 52 15 - - PUNCT admet-389 52 16 pcdna3.1 pcdna3.1 VERB admet-389 52 17 plasmid plasmid ADJ admet-389 52 18 dna dna NOUN admet-389 52 19 and and CCONJ admet-389 52 20 10 10 NUM admet-389 52 21 µg µg NOUN admet-389 52 22 of of ADP admet-389 52 23 salmon salmon NOUN admet-389 52 24 sperm sperm NOUN admet-389 52 25 dna dna NOUN admet-389 52 26 in in ADP admet-389 52 27 a a DET admet-389 52 28 cuvette cuvette NOUN admet-389 52 29 ( ( PUNCT admet-389 52 30 4 4 NUM admet-389 52 31 - - PUNCT admet-389 52 32 mm mm NUM admet-389 52 33 gap gap NOUN admet-389 52 34 ) ) PUNCT admet-389 52 35 using use VERB admet-389 52 36 a a DET admet-389 52 37 btx btx PROPN admet-389 52 38 ecm ecm NOUN admet-389 52 39 630 630 NUM admet-389 52 40 electroporator electroporator NOUN admet-389 52 41 ( ( PUNCT admet-389 52 42 harvard harvard PROPN admet-389 52 43 apparatus apparatus PROPN admet-389 52 44 , , PUNCT admet-389 52 45 st st PROPN admet-389 52 46 . . PROPN admet-389 52 47 laurent laurent PROPN admet-389 52 48 , , PUNCT admet-389 52 49 quebec quebec PROPN admet-389 52 50 , , PUNCT admet-389 52 51 canada canada PROPN admet-389 52 52 ) ) PUNCT admet-389 52 53 with with ADP admet-389 52 54 settings setting NOUN admet-389 52 55 of of ADP admet-389 52 56 1050 1050 NUM admet-389 52 57 microfarad microfarad NOUN admet-389 52 58 , , PUNCT admet-389 52 59 260 260 NUM admet-389 52 60 v v NOUN admet-389 52 61 , , PUNCT admet-389 52 62 and and CCONJ admet-389 52 63 at at ADP admet-389 52 64 no no DET admet-389 52 65 resistance resistance NOUN admet-389 52 66 . . PUNCT admet-389 53 1 forty forty NUM admet-389 53 2 -eight -eight NOUN admet-389 53 3 hours hour NOUN admet-389 53 4 after after ADP admet-389 53 5 the the DET admet-389 53 6 electroporation electroporation NOUN admet-389 53 7 , , PUNCT admet-389 53 8 positively positively ADV admet-389 53 9 transfected transfecte VERB admet-389 53 10 cells cell NOUN admet-389 53 11 were be AUX admet-389 53 12 selected select VERB admet-389 53 13 with with ADP admet-389 53 14 200 200 NUM admet-389 53 15 µg µg NOUN admet-389 53 16 / / SYM admet-389 53 17 ml ml PROPN admet-389 53 18 hygromycin hygromycin PROPN admet-389 53 19 b. b. PROPN admet-389 54 1 the the DET admet-389 54 2 cho cho PROPN admet-389 54 3 wild wild ADJ admet-389 54 4 type type NOUN admet-389 54 5 were be AUX admet-389 54 6 not not PART admet-389 54 7 transfected transfecte VERB admet-389 54 8 with with ADP admet-389 54 9 hoct2 hoct2 NOUN admet-389 54 10 as as SCONJ admet-389 54 11 the the DET admet-389 54 12 cells cell NOUN admet-389 54 13 will will AUX admet-389 54 14 be be AUX admet-389 54 15 used use VERB admet-389 54 16 as as ADP admet-389 54 17 the the DET admet-389 54 18 control control NOUN admet-389 54 19 . . PUNCT admet-389 55 1 the the DET admet-389 55 2 cho cho PROPN admet-389 55 3 cells cell NOUN admet-389 55 4 were be AUX admet-389 55 5 grown grow VERB admet-389 55 6 in in ADP admet-389 55 7 ham ham PROPN admet-389 55 8 's 's PART admet-389 55 9 f-12 f-12 NOUN admet-389 55 10 ( ( PUNCT admet-389 55 11 kaighn kaighn PROPN admet-389 55 12 's 's PART admet-389 55 13 modification modification NOUN admet-389 55 14 ) ) PUNCT admet-389 55 15 containing contain VERB admet-389 55 16 10 10 NUM admet-389 55 17 % % NOUN admet-389 55 18 fbs fb VERB admet-389 55 19 , , PUNCT admet-389 55 20 1 1 NUM admet-389 55 21 % % NOUN admet-389 55 22 penicillinstreptomycin penicillinstreptomycin VERB admet-389 55 23 , , PUNCT admet-389 55 24 200 200 NUM admet-389 55 25 µg/ µg/ NOUN admet-389 55 26 ml ml ADP admet-389 55 27 hygromicin hygromicin PROPN admet-389 55 28 b b PROPN admet-389 55 29 ( ( PUNCT admet-389 55 30 transfected transfecte VERB admet-389 55 31 ) ) PUNCT admet-389 55 32 and and CCONJ admet-389 55 33 100 100 NUM admet-389 55 34 µg µg ADV admet-389 55 35 /ml /ml PUNCT admet-389 55 36 zeocin zeocin PROPN admet-389 55 37 ( ( PUNCT admet-389 55 38 wild wild NOUN admet-389 55 39 - - PUNCT admet-389 55 40 type type NOUN admet-389 55 41 ) ) PUNCT admet-389 55 42 , , PUNCT admet-389 55 43 respectively respectively ADV admet-389 55 44 . . PUNCT admet-389 56 1 both both DET admet-389 56 2 cell cell NOUN admet-389 56 3 types type NOUN admet-389 56 4 were be AUX admet-389 56 5 maintained maintain VERB admet-389 56 6 in in ADP admet-389 56 7 a a DET admet-389 56 8 humidified humidify VERB admet-389 56 9 atmosphere atmosphere NOUN admet-389 56 10 ( ( PUNCT admet-389 56 11 5 5 NUM admet-389 56 12 % % NOUN admet-389 56 13 co2/95 co2/95 NOUN admet-389 56 14 % % NOUN admet-389 56 15 o2 o2 PROPN admet-389 56 16 ) ) PUNCT admet-389 56 17 . . PUNCT admet-389 57 1 the the DET admet-389 57 2 cells cell NOUN admet-389 57 3 were be AUX admet-389 57 4 passaged passage VERB admet-389 57 5 every every DET admet-389 57 6 2 2 NUM admet-389 57 7 - - SYM admet-389 57 8 3 3 NUM admet-389 57 9 days day NOUN admet-389 57 10 . . PUNCT admet-389 58 1 cells cell NOUN admet-389 58 2 within within ADP admet-389 58 3 passage passage NOUN admet-389 58 4 4–35 4–35 PROPN admet-389 58 5 were be AUX admet-389 58 6 used use VERB admet-389 58 7 for for ADP admet-389 58 8 the the DET admet-389 58 9 studies study NOUN admet-389 58 10 . . PUNCT admet-389 59 1 uptake uptake ADJ admet-389 59 2 studies study NOUN admet-389 59 3 confluent confluent ADJ admet-389 59 4 cho cho ADJ admet-389 59 5 - - PUNCT admet-389 59 6 hoct2 hoct2 NOUN admet-389 59 7 and and CCONJ admet-389 59 8 wild wild ADJ admet-389 59 9 – – PUNCT admet-389 59 10 type type NOUN admet-389 59 11 cells cell NOUN admet-389 59 12 cultured cultured ADJ admet-389 59 13 in in ADP admet-389 59 14 24 24 NUM admet-389 59 15 , , PUNCT admet-389 59 16 48or 48or ADJ admet-389 59 17 96 96 NUM admet-389 59 18 well well ADJ admet-389 59 19 plates plate NOUN admet-389 59 20 were be AUX admet-389 59 21 used use VERB admet-389 59 22 for for ADP admet-389 59 23 uptake uptake ADJ admet-389 59 24 experiments experiment NOUN admet-389 59 25 . . PUNCT admet-389 60 1 the the DET admet-389 60 2 uptake uptake ADJ admet-389 60 3 buffer buffer NOUN admet-389 60 4 was be AUX admet-389 60 5 prepared prepare VERB admet-389 60 6 with with ADP admet-389 60 7 hbss hbss NOUN admet-389 60 8 supplemented supplement VERB admet-389 60 9 with with ADP admet-389 60 10 25 25 NUM admet-389 60 11 mm mm NOUN admet-389 60 12 glucose glucose NOUN admet-389 60 13 and and CCONJ admet-389 60 14 20 20 NUM admet-389 60 15 mm mm NOUN admet-389 60 16 hepes hepe NOUN admet-389 60 17 ( ( PUNCT admet-389 60 18 ph ph NOUN admet-389 60 19 7.4 7.4 NUM admet-389 60 20 ) ) PUNCT admet-389 60 21 . . PUNCT admet-389 61 1 the the DET admet-389 61 2 cells cell NOUN admet-389 61 3 in in ADP admet-389 61 4 each each DET admet-389 61 5 well well NOUN admet-389 61 6 were be AUX admet-389 61 7 pre pre VERB admet-389 61 8 - - VERB admet-389 61 9 incubated incubate VERB admet-389 61 10 in in ADP admet-389 61 11 substrate substrate NOUN admet-389 61 12 - - PUNCT admet-389 61 13 free free ADJ admet-389 61 14 uptake uptake ADJ admet-389 61 15 buffer buffer NOUN admet-389 61 16 ( ( PUNCT admet-389 61 17 200 200 NUM admet-389 61 18 µl µl NOUN admet-389 61 19 ) ) PUNCT admet-389 61 20 at at ADP admet-389 61 21 37 37 NUM admet-389 61 22 ° ° ADP admet-389 61 23 c c NOUN admet-389 61 24 for for ADP admet-389 61 25 15 15 NUM admet-389 61 26 min min NOUN admet-389 61 27 . . PROPN admet-389 61 28 time time NOUN admet-389 61 29 - - PUNCT admet-389 61 30 dependent dependent ADJ admet-389 61 31 studies study NOUN admet-389 61 32 were be AUX admet-389 61 33 conducted conduct VERB admet-389 61 34 by by ADP admet-389 61 35 incubating incubate VERB admet-389 61 36 cell cell NOUN admet-389 61 37 monolayers monolayer NOUN admet-389 61 38 with with ADP admet-389 61 39 10 10 NUM admet-389 61 40 µm µm ADP admet-389 61 41 cationic cationic ADJ admet-389 61 42 compounds compound NOUN admet-389 61 43 for for ADP admet-389 61 44 up up ADP admet-389 61 45 to to PART admet-389 61 46 60 60 NUM admet-389 61 47 min min NOUN admet-389 61 48 at at ADP admet-389 61 49 37 37 NUM admet-389 61 50 c c NUM admet-389 61 51 . . PUNCT admet-389 62 1 uptake uptake ADJ admet-389 62 2 studies study NOUN admet-389 62 3 were be AUX admet-389 62 4 initiated initiate VERB admet-389 62 5 by by ADP admet-389 62 6 replacing replace VERB admet-389 62 7 the the DET admet-389 62 8 pre pre ADJ admet-389 62 9 - - ADJ admet-389 62 10 incubation incubation ADJ admet-389 62 11 substrate substrate NOUN admet-389 62 12 - - PUNCT admet-389 62 13 free free ADJ admet-389 62 14 buffer buffer NOUN admet-389 62 15 with with ADP admet-389 62 16 buffer buffer NOUN admet-389 62 17 solutions solution NOUN admet-389 62 18 containing contain VERB admet-389 62 19 200 200 NUM admet-389 62 20 µl µl NOUN admet-389 62 21 of of ADP admet-389 62 22 rhodamine rhodamine NOUN admet-389 62 23 123 123 NUM admet-389 62 24 , , PUNCT admet-389 62 25 rhodamine rhodamine NOUN admet-389 62 26 6 6 NUM admet-389 62 27 g g NOUN admet-389 62 28 , , PUNCT admet-389 62 29 and and CCONJ admet-389 62 30 amiloride amiloride NOUN admet-389 62 31 , , PUNCT admet-389 62 32 respectively respectively ADV admet-389 62 33 . . PUNCT admet-389 63 1 based base VERB admet-389 63 2 on on ADP admet-389 63 3 preliminary preliminary ADJ admet-389 63 4 studies study NOUN admet-389 63 5 , , PUNCT admet-389 63 6 the the DET admet-389 63 7 experiments experiment NOUN admet-389 63 8 were be AUX admet-389 63 9 terminated terminate VERB admet-389 63 10 at at ADP admet-389 63 11 1 1 NUM admet-389 63 12 , , PUNCT admet-389 63 13 2 2 NUM admet-389 63 14 , , PUNCT admet-389 63 15 5 5 NUM admet-389 63 16 , , PUNCT admet-389 63 17 15 15 NUM admet-389 63 18 , , PUNCT admet-389 63 19 30 30 NUM admet-389 63 20 and and CCONJ admet-389 63 21 60 60 NUM admet-389 63 22 min min NOUN admet-389 63 23 by by ADP admet-389 63 24 immediately immediately ADV admet-389 63 25 removing remove VERB admet-389 63 26 the the DET admet-389 63 27 compounds compound NOUN admet-389 63 28 and and CCONJ admet-389 63 29 washing wash VERB admet-389 63 30 the the DET admet-389 63 31 cells cell NOUN admet-389 63 32 three three NUM admet-389 63 33 times time NOUN admet-389 63 34 with with ADP admet-389 63 35 ice ice NOUN admet-389 63 36 - - PUNCT admet-389 63 37 cold cold ADJ admet-389 63 38 hbss hbss NOUN admet-389 63 39 . . PUNCT admet-389 64 1 subsequently subsequently ADV admet-389 64 2 , , PUNCT admet-389 64 3 the the DET admet-389 64 4 cells cell NOUN admet-389 64 5 were be AUX admet-389 64 6 dissolved dissolve VERB admet-389 64 7 in in ADP admet-389 64 8 200 200 NUM admet-389 64 9 μl μl ADP admet-389 64 10 of of ADP admet-389 64 11 1 1 NUM admet-389 64 12 % % NOUN admet-389 64 13 triton triton PROPN admet-389 64 14 x-100r x-100r PROPN admet-389 64 15 containing contain VERB admet-389 64 16 0.1 0.1 NUM admet-389 64 17 m m PROPN admet-389 64 18 naoh naoh NOUN admet-389 64 19 on on ADP admet-389 64 20 a a DET admet-389 64 21 shaker shaker NOUN admet-389 64 22 for for ADP admet-389 64 23 1–2 1–2 NUM admet-389 64 24 hours hour NOUN admet-389 64 25 at at ADP admet-389 64 26 37 37 NUM admet-389 64 27 ° ° NOUN admet-389 64 28 c c NOUN admet-389 64 29 . . PUNCT admet-389 65 1 furthermore furthermore ADV admet-389 65 2 , , PUNCT admet-389 65 3 uptake uptake NOUN admet-389 65 4 of of ADP admet-389 65 5 the the DET admet-389 65 6 fluorescent fluorescent NOUN admet-389 65 7 substrates substrate NOUN admet-389 65 8 was be AUX admet-389 65 9 investigated investigate VERB admet-389 65 10 at at ADP admet-389 65 11 different different ADJ admet-389 65 12 concentrations concentration NOUN admet-389 65 13 ( ( PUNCT admet-389 65 14 1 1 NUM admet-389 65 15 - - SYM admet-389 65 16 3000 3000 NUM admet-389 65 17 µm µm NOUN admet-389 65 18 ) ) PUNCT admet-389 65 19 and and CCONJ admet-389 65 20 ph ph ADJ admet-389 65 21 values value NOUN admet-389 65 22 ( ( PUNCT admet-389 65 23 5.0 5.0 NUM admet-389 65 24 - - SYM admet-389 65 25 8.5 8.5 NUM admet-389 65 26 ) ) PUNCT admet-389 65 27 . . PUNCT admet-389 66 1 in in ADP admet-389 66 2 studies study NOUN admet-389 66 3 involving involve VERB admet-389 66 4 inhibition inhibition NOUN admet-389 66 5 , , PUNCT admet-389 66 6 the the DET admet-389 66 7 cells cell NOUN admet-389 66 8 were be AUX admet-389 66 9 preincubated preincubate VERB admet-389 66 10 at at ADP admet-389 66 11 37 37 NUM admet-389 66 12 ° ° NOUN admet-389 66 13 c c NOUN admet-389 66 14 with with ADP admet-389 66 15 or or CCONJ admet-389 66 16 without without ADP admet-389 66 17 inhibitors inhibitor NOUN admet-389 66 18 for for ADP admet-389 66 19 15 15 NUM admet-389 66 20 min min NOUN admet-389 66 21 prior prior ADV admet-389 66 22 to to ADP admet-389 66 23 the the DET admet-389 66 24 uptake uptake ADJ admet-389 66 25 studies study NOUN admet-389 66 26 . . PUNCT admet-389 67 1 each each DET admet-389 67 2 set set NOUN admet-389 67 3 of of ADP admet-389 67 4 experiments experiment NOUN admet-389 67 5 was be AUX admet-389 67 6 performed perform VERB admet-389 67 7 with with ADP admet-389 67 8 cells cell NOUN admet-389 67 9 of of ADP admet-389 67 10 the the DET admet-389 67 11 same same ADJ admet-389 67 12 age age NOUN admet-389 67 13 and and CCONJ admet-389 67 14 passage passage NOUN admet-389 67 15 . . PUNCT admet-389 68 1 sample sample NOUN admet-389 68 2 analyses analyse VERB admet-389 68 3 rhodamine-123 rhodamine-123 NOUN admet-389 68 4 , , PUNCT admet-389 68 5 rhodamine-6 rhodamine-6 PROPN admet-389 68 6 g g NOUN admet-389 68 7 , , PUNCT admet-389 68 8 and and CCONJ admet-389 68 9 amiloride amiloride ADV admet-389 68 10 in in ADP admet-389 68 11 samples sample NOUN admet-389 68 12 were be AUX admet-389 68 13 quantified quantify VERB admet-389 68 14 with with ADP admet-389 68 15 modulus modulus ADJ admet-389 68 16 single single ADJ admet-389 68 17 tube tube NOUN admet-389 68 18 multimode multimode NOUN admet-389 68 19 florescence florescence NOUN admet-389 68 20 reader reader NOUN admet-389 68 21 ( ( PUNCT admet-389 68 22 fluorimeter fluorimeter NOUN admet-389 68 23 model model NOUN admet-389 68 24 9200 9200 NUM admet-389 68 25 , , PUNCT admet-389 68 26 turner turner PROPN admet-389 68 27 bio bio PROPN admet-389 68 28 systems systems PROPN admet-389 68 29 , , PUNCT admet-389 68 30 sunnyvale sunnyvale PROPN admet-389 68 31 , , PUNCT admet-389 68 32 ca ca PROPN admet-389 68 33 , , PUNCT admet-389 68 34 usa usa PROPN admet-389 68 35 ) ) PUNCT admet-389 68 36 using use VERB admet-389 68 37 the the DET admet-389 68 38 green green ADJ admet-389 68 39 , , PUNCT admet-389 68 40 blue blue ADJ admet-389 68 41 and and CCONJ admet-389 68 42 uv uv NOUN admet-389 68 43 filter filter NOUN admet-389 68 44 configurations configuration NOUN admet-389 68 45 for for ADP admet-389 68 46 rhodamines rhodamine NOUN admet-389 68 47 , , PUNCT admet-389 68 48 and and CCONJ admet-389 68 49 amiloride amiloride NOUN admet-389 68 50 , , PUNCT admet-389 68 51 respectively respectively ADV admet-389 68 52 . . PUNCT admet-389 69 1 protein protein NOUN admet-389 69 2 content content NOUN admet-389 69 3 of of ADP admet-389 69 4 cell cell NOUN admet-389 69 5 lysates lysate NOUN admet-389 69 6 was be AUX admet-389 69 7 measured measure VERB admet-389 69 8 using use VERB admet-389 69 9 bovine bovine NOUN admet-389 69 10 serum serum NOUN admet-389 69 11 albumin albumin NOUN admet-389 69 12 as as ADP admet-389 69 13 standards standard NOUN admet-389 69 14 according accord VERB admet-389 69 15 to to ADP admet-389 69 16 bca bca PROPN admet-389 69 17 protein protein NOUN admet-389 69 18 assay assay NOUN admet-389 69 19 protocol protocol NOUN admet-389 69 20 so so SCONJ admet-389 69 21 as as SCONJ admet-389 69 22 to to PART admet-389 69 23 normalize normalize VERB admet-389 69 24 the the DET admet-389 69 25 cell cell NOUN admet-389 69 26 population population NOUN admet-389 69 27 / / SYM admet-389 69 28 density density NOUN admet-389 69 29 in in ADP admet-389 69 30 each each DET admet-389 69 31 plate plate NOUN admet-389 69 32 [ [ X admet-389 69 33 19 19 NUM admet-389 69 34 ] ] PUNCT admet-389 69 35 . . PUNCT admet-389 70 1 data datum NOUN admet-389 70 2 analyses analysis NOUN admet-389 70 3 . . PUNCT admet-389 71 1 the the DET admet-389 71 2 saturable saturable ADJ admet-389 71 3 uptake uptake NOUN admet-389 71 4 of of ADP admet-389 71 5 the the DET admet-389 71 6 fluorescent fluorescent NOUN admet-389 71 7 substrates substrate NOUN admet-389 71 8 was be AUX admet-389 71 9 analyzed analyze VERB admet-389 71 10 by by ADP admet-389 71 11 assuming assume VERB admet-389 71 12 michaelis michaeli NOUN admet-389 71 13 - - PUNCT admet-389 71 14 menten menten ADJ admet-389 71 15 type type NOUN admet-389 71 16 carrier carrier NOUN admet-389 71 17 - - PUNCT admet-389 71 18 mediated mediate VERB admet-389 71 19 transport transport NOUN admet-389 71 20 . . PUNCT admet-389 72 1 the the DET admet-389 72 2 maximum maximum ADJ admet-389 72 3 transport transport NOUN admet-389 72 4 rate rate NOUN admet-389 72 5 ( ( PUNCT admet-389 72 6 vmax vmax PROPN admet-389 72 7 ) ) PUNCT admet-389 72 8 , , PUNCT admet-389 72 9 the the DET admet-389 72 10 michaelis michaelis PROPN admet-389 72 11 constant constant ADJ admet-389 72 12 ( ( PUNCT admet-389 72 13 km km NOUN admet-389 72 14 ) ) PUNCT admet-389 72 15 , , PUNCT admet-389 72 16 and and CCONJ admet-389 72 17 ic50 ic50 PROPN admet-389 72 18 for for ADP admet-389 72 19 the the DET admet-389 72 20 inhibition inhibition NOUN admet-389 72 21 studies study NOUN admet-389 72 22 were be AUX admet-389 72 23 determined determine VERB admet-389 72 24 by by ADP admet-389 72 25 non non ADJ admet-389 72 26 - - ADJ admet-389 72 27 linear linear ADJ admet-389 72 28 regression regression NOUN admet-389 72 29 using use VERB admet-389 72 30 prism(r prism(r NOUN admet-389 72 31 ) ) PUNCT admet-389 72 32 5.0 5.0 NUM admet-389 72 33 ( ( PUNCT admet-389 72 34 graphpad graphpad NOUN admet-389 72 35 , , PUNCT admet-389 72 36 san san PROPN admet-389 72 37 diego diego PROPN admet-389 72 38 , , PUNCT admet-389 72 39 ca ca PROPN admet-389 72 40 , , PUNCT admet-389 72 41 usa usa PROPN admet-389 72 42 ) ) PUNCT admet-389 72 43 . . PUNCT admet-389 73 1 passive passive ADJ admet-389 73 2 diffusion diffusion NOUN admet-389 73 3 was be AUX admet-389 73 4 accounted account VERB admet-389 73 5 for for ADP admet-389 73 6 by by ADP admet-389 73 7 subtracting subtract VERB admet-389 73 8 the the DET admet-389 73 9 rate rate NOUN admet-389 73 10 constant constant ADJ admet-389 73 11 for for ADP admet-389 73 12 the the DET admet-389 73 13 compounds compound NOUN admet-389 73 14 passive passive ADJ admet-389 73 15 diffusion diffusion NOUN admet-389 73 16 in in ADP admet-389 73 17 cho cho ADJ admet-389 73 18 - - PUNCT admet-389 73 19 wild wild ADJ admet-389 73 20 cells cell NOUN admet-389 73 21 from from ADP admet-389 73 22 cho cho ADJ admet-389 73 23 - - PUNCT admet-389 73 24 hoct2 hoct2 NOUN admet-389 73 25 - - PUNCT admet-389 73 26 expressing express VERB admet-389 73 27 cells cell NOUN admet-389 73 28 . . PUNCT admet-389 74 1 unless unless SCONJ admet-389 74 2 stated state VERB admet-389 74 3 otherwise otherwise ADV admet-389 74 4 , , PUNCT admet-389 74 5 all all PRON admet-389 74 6 experiments experiment NOUN admet-389 74 7 remigius remigius PROPN admet-389 74 8 agu agu PROPN admet-389 74 9 et et PROPN admet-389 74 10 al al PROPN admet-389 74 11 . . PROPN admet-389 74 12 admet admet PROPN admet-389 74 13 & & CCONJ admet-389 74 14 dmpk dmpk PROPN admet-389 74 15 5(2 5(2 NUM admet-389 74 16 ) ) PUNCT admet-389 74 17 ( ( PUNCT admet-389 74 18 2017 2017 NUM admet-389 74 19 ) ) PUNCT admet-389 74 20 135 135 NUM admet-389 74 21 - - SYM admet-389 74 22 145 145 NUM admet-389 74 23 138 138 NUM admet-389 74 24 were be AUX admet-389 74 25 performed perform VERB admet-389 74 26 in in ADP admet-389 74 27 triplicates triplicate NOUN admet-389 74 28 and and CCONJ admet-389 74 29 data datum NOUN admet-389 74 30 presented present VERB admet-389 74 31 as as ADP admet-389 74 32 mean mean NOUN admet-389 74 33 ± ± NUM admet-389 74 34 sd sd NOUN admet-389 74 35 . . PUNCT admet-389 75 1 where where SCONJ admet-389 75 2 appropriate appropriate ADJ admet-389 75 3 , , PUNCT admet-389 75 4 statistical statistical ADJ admet-389 75 5 significance significance NOUN admet-389 75 6 of of ADP admet-389 75 7 the the DET admet-389 75 8 results result NOUN admet-389 75 9 was be AUX admet-389 75 10 determined determine VERB admet-389 75 11 with with ADP admet-389 75 12 anova anova PROPN admet-389 75 13 using use VERB admet-389 75 14 instat instat PROPN admet-389 75 15 ® ® NOUN admet-389 75 16 3.0 3.0 NUM admet-389 75 17 ( ( PUNCT admet-389 75 18 graphpad graphpad NOUN admet-389 75 19 , , PUNCT admet-389 75 20 san san PROPN admet-389 75 21 diego diego PROPN admet-389 75 22 , , PUNCT admet-389 75 23 ca ca PROPN admet-389 75 24 , , PUNCT admet-389 75 25 usa usa PROPN admet-389 75 26 ) ) PUNCT admet-389 75 27 . . PUNCT admet-389 76 1 p p X admet-389 76 2 < < X admet-389 76 3 0.05 0.05 NUM admet-389 76 4 was be AUX admet-389 76 5 considered consider VERB admet-389 76 6 significant significant ADJ admet-389 76 7 . . PUNCT admet-389 77 1 the the DET admet-389 77 2 specific specific ADJ admet-389 77 3 uptake uptake NOUN admet-389 77 4 of of ADP admet-389 77 5 the the DET admet-389 77 6 dyes dye NOUN admet-389 77 7 was be AUX admet-389 77 8 calculated calculate VERB admet-389 77 9 by by ADP admet-389 77 10 subtracting subtract VERB admet-389 77 11 the the DET admet-389 77 12 unspecific unspecific ADJ admet-389 77 13 uptake uptake NOUN admet-389 77 14 in in ADP admet-389 77 15 wild wild ADJ admet-389 77 16 cho cho NOUN admet-389 77 17 cells cell NOUN admet-389 77 18 from from ADP admet-389 77 19 the the DET admet-389 77 20 total total ADJ admet-389 77 21 uptake uptake NOUN admet-389 77 22 in in ADP admet-389 77 23 cho cho ADJ admet-389 77 24 - - PUNCT admet-389 77 25 hoct2 hoct2 NOUN admet-389 77 26 cells cell NOUN admet-389 77 27 . . PUNCT admet-389 78 1 results result NOUN admet-389 78 2 and and CCONJ admet-389 78 3 discussion discussion NOUN admet-389 78 4 considering consider VERB admet-389 78 5 the the DET admet-389 78 6 fluorescence fluorescence ADJ admet-389 78 7 characteristics characteristic NOUN admet-389 78 8 of of ADP admet-389 78 9 rhodamine-123 rhodamine-123 NOUN admet-389 78 10 and and CCONJ admet-389 78 11 rhodamine-6 rhodamine-6 PROPN admet-389 78 12 g g NOUN admet-389 78 13 ( ( PUNCT admet-389 78 14 high high ADJ admet-389 78 15 quantum quantum NOUN admet-389 78 16 yield yield NOUN admet-389 78 17 , , PUNCT admet-389 78 18 long long ADJ admet-389 78 19 emission emission NOUN admet-389 78 20 and and CCONJ admet-389 78 21 excitation excitation NOUN admet-389 78 22 wavelengths wavelength NOUN admet-389 78 23 ) ) PUNCT admet-389 78 24 their their PRON admet-389 78 25 extensive extensive ADJ admet-389 78 26 current current ADJ admet-389 78 27 biomedical biomedical ADJ admet-389 78 28 applications application NOUN admet-389 78 29 , , PUNCT admet-389 78 30 and and CCONJ admet-389 78 31 the the DET admet-389 78 32 fact fact NOUN admet-389 78 33 that that SCONJ admet-389 78 34 these these DET admet-389 78 35 compounds compound NOUN admet-389 78 36 are be AUX admet-389 78 37 organic organic ADJ admet-389 78 38 cations cation NOUN admet-389 78 39 [ [ X admet-389 78 40 17 17 NUM admet-389 78 41 ] ] PUNCT admet-389 78 42 , , PUNCT admet-389 78 43 we we PRON admet-389 78 44 selected select VERB admet-389 78 45 them they PRON admet-389 78 46 for for ADP admet-389 78 47 this this DET admet-389 78 48 study study NOUN admet-389 78 49 . . PUNCT admet-389 79 1 furthermore furthermore ADV admet-389 79 2 , , PUNCT admet-389 79 3 rhodamine rhodamine PROPN admet-389 79 4 123 123 NUM admet-389 79 5 was be AUX admet-389 79 6 used use VERB admet-389 79 7 based base VERB admet-389 79 8 on on ADP admet-389 79 9 the the DET admet-389 79 10 fact fact NOUN admet-389 79 11 that that SCONJ admet-389 79 12 it it PRON admet-389 79 13 is be AUX admet-389 79 14 an an DET admet-389 79 15 organic organic ADJ admet-389 79 16 cation cation NOUN admet-389 79 17 transporter transporter NOUN admet-389 79 18 used use VERB admet-389 79 19 for for ADP admet-389 79 20 characterizing characterize VERB admet-389 79 21 oct2 oct2 NOUN admet-389 80 1 [ [ X admet-389 80 2 20 20 NUM admet-389 80 3 ] ] PUNCT admet-389 80 4 amiloride amiloride NOUN admet-389 80 5 was be AUX admet-389 80 6 chosen choose VERB admet-389 80 7 because because SCONJ admet-389 80 8 it it PRON admet-389 80 9 is be AUX admet-389 80 10 the the DET admet-389 80 11 only only ADJ admet-389 80 12 oct2 oct2 NOUN admet-389 80 13 substrate substrate NOUN admet-389 80 14 with with ADP admet-389 80 15 desirable desirable ADJ admet-389 80 16 fluorescent fluorescent ADJ admet-389 80 17 characteristics characteristic NOUN admet-389 80 18 listed list VERB admet-389 80 19 by by ADP admet-389 80 20 the the DET admet-389 80 21 international international ADJ admet-389 80 22 transporters transporter NOUN admet-389 80 23 consortium consortium NOUN admet-389 80 24 for for ADP admet-389 80 25 oct2 oct2 NOUN admet-389 80 26 transporter transporter NOUN admet-389 80 27 characterization characterization NOUN admet-389 80 28 [ [ X admet-389 80 29 18 18 NUM admet-389 80 30 ] ] PUNCT admet-389 80 31 . . PUNCT admet-389 81 1 the the DET admet-389 81 2 hoct2 hoct2 NOUN admet-389 81 3 is be AUX admet-389 81 4 a a DET admet-389 81 5 very very ADV admet-389 81 6 important important ADJ admet-389 81 7 oct oct NOUN admet-389 81 8 isoform isoform NOUN admet-389 81 9 that that PRON admet-389 81 10 exhibits exhibit VERB admet-389 81 11 clinically clinically ADV admet-389 81 12 relevant relevant ADJ admet-389 81 13 genetic genetic ADJ admet-389 81 14 polymorphisms polymorphism NOUN admet-389 81 15 , , PUNCT admet-389 81 16 participates participate VERB admet-389 81 17 in in ADP admet-389 81 18 clinical clinical ADJ admet-389 81 19 drug drug NOUN admet-389 81 20 – – PUNCT admet-389 81 21 drug drug NOUN admet-389 81 22 interactions interaction NOUN admet-389 81 23 and and CCONJ admet-389 81 24 alters alter VERB admet-389 81 25 the the DET admet-389 81 26 disposition disposition NOUN admet-389 81 27 and and CCONJ admet-389 81 28 excretion excretion NOUN admet-389 81 29 of of ADP admet-389 81 30 some some DET admet-389 81 31 drugs drug NOUN admet-389 81 32 [ [ X admet-389 81 33 18 18 NUM admet-389 81 34 ] ] PUNCT admet-389 81 35 . . PUNCT admet-389 82 1 figure figure NOUN admet-389 82 2 1 1 NUM admet-389 82 3 . . PUNCT admet-389 82 4 time time NOUN admet-389 82 5 - - PUNCT admet-389 82 6 dependent dependent ADJ admet-389 82 7 uptake uptake NOUN admet-389 82 8 of of ADP admet-389 82 9 the the DET admet-389 82 10 fluorescent fluorescent NOUN admet-389 82 11 compounds compound VERB admet-389 82 12 into into ADP admet-389 82 13 cho cho PROPN admet-389 82 14 - - PUNCT admet-389 82 15 hoct2 hoct2 PROPN admet-389 82 16 . . PUNCT admet-389 83 1 cell cell NOUN admet-389 83 2 layers layer NOUN admet-389 83 3 were be AUX admet-389 83 4 incubated incubate VERB admet-389 83 5 with with ADP admet-389 83 6 ( ( PUNCT admet-389 83 7 10 10 NUM admet-389 83 8 m m NOUN admet-389 83 9 ) ) PUNCT admet-389 83 10 of of ADP admet-389 83 11 each each DET admet-389 83 12 compound compound NOUN admet-389 83 13 as as SCONJ admet-389 83 14 indicated indicate VERB admet-389 83 15 . . PUNCT admet-389 84 1 subsequently subsequently ADV admet-389 84 2 , , PUNCT admet-389 84 3 the the DET admet-389 84 4 cells cell NOUN admet-389 84 5 were be AUX admet-389 84 6 washed wash VERB admet-389 84 7 with with ADP admet-389 84 8 ice ice NOUN admet-389 84 9 - - PUNCT admet-389 84 10 cold cold ADJ admet-389 84 11 uptake uptake ADJ admet-389 84 12 buffer buffer NOUN admet-389 84 13 followed follow VERB admet-389 84 14 by by ADP admet-389 84 15 extraction extraction NOUN admet-389 84 16 with with ADP admet-389 84 17 triton triton PROPN admet-389 84 18 x-100/0.1 x-100/0.1 PROPN admet-389 84 19 m m PROPN admet-389 84 20 naoh naoh PROPN admet-389 84 21 . . PUNCT admet-389 85 1 data datum NOUN admet-389 85 2 represent represent VERB admet-389 85 3 mean mean PROPN admet-389 85 4 ± ± NUM admet-389 85 5 sd sd NOUN admet-389 85 6 ; ; PUNCT admet-389 85 7 n n NOUN admet-389 85 8 = = SYM admet-389 85 9 3 3 X admet-389 85 10 . . X admet-389 85 11 figure figure NOUN admet-389 85 12 1 1 NUM admet-389 85 13 shows show VERB admet-389 85 14 the the DET admet-389 85 15 time time NOUN admet-389 85 16 – – PUNCT admet-389 85 17 dependent dependent ADJ admet-389 85 18 uptake uptake NOUN admet-389 85 19 of of ADP admet-389 85 20 the the DET admet-389 85 21 substrates substrate NOUN admet-389 85 22 by by ADP admet-389 85 23 cho cho NOUN admet-389 85 24 cells cell NOUN admet-389 85 25 stably stably ADV admet-389 85 26 expressing express VERB admet-389 85 27 hoct2 hoct2 NOUN admet-389 85 28 . . PUNCT admet-389 86 1 uptake uptake NOUN admet-389 86 2 of of ADP admet-389 86 3 the the DET admet-389 86 4 three three NUM admet-389 86 5 compounds compound NOUN admet-389 86 6 was be AUX admet-389 86 7 linear linear ADJ admet-389 86 8 up up ADP admet-389 86 9 to to ADP admet-389 86 10 approximately approximately ADV admet-389 86 11 7 7 NUM admet-389 86 12 min min NOUN admet-389 86 13 ; ; PUNCT admet-389 86 14 after after ADP admet-389 86 15 which which PRON admet-389 86 16 a a DET admet-389 86 17 gradual gradual ADJ admet-389 86 18 plateau plateau NOUN admet-389 86 19 was be AUX admet-389 86 20 observed observe VERB admet-389 86 21 . . PUNCT admet-389 87 1 based base VERB admet-389 87 2 on on ADP admet-389 87 3 these these DET admet-389 87 4 observations observation NOUN admet-389 87 5 , , PUNCT admet-389 87 6 an an DET admet-389 87 7 incubation incubation NOUN admet-389 87 8 period period NOUN admet-389 87 9 of of ADP admet-389 87 10 5 5 NUM admet-389 87 11 min min NOUN admet-389 87 12 was be AUX admet-389 87 13 chosen choose VERB admet-389 87 14 for for ADP admet-389 87 15 subsequent subsequent ADJ admet-389 87 16 experiments experiment NOUN admet-389 87 17 . . PUNCT admet-389 88 1 the the DET admet-389 88 2 effect effect NOUN admet-389 88 3 of of ADP admet-389 88 4 extracellular extracellular ADJ admet-389 88 5 ph ph NOUN admet-389 88 6 on on ADP admet-389 88 7 the the DET admet-389 88 8 uptake uptake NOUN admet-389 88 9 of of ADP admet-389 88 10 amiloride amiloride NOUN admet-389 88 11 , , PUNCT admet-389 88 12 rhodamine rhodamine NOUN admet-389 88 13 6 6 NUM admet-389 88 14 g g NOUN admet-389 88 15 and and CCONJ admet-389 88 16 rhodamine rhodamine NOUN admet-389 88 17 123 123 NUM admet-389 88 18 by by ADP admet-389 88 19 cho cho PROPN admet-389 88 20 - - PUNCT admet-389 88 21 hoct2 hoct2 PROPN admet-389 88 22 and and CCONJ admet-389 88 23 cho cho PROPN admet-389 88 24 - - PUNCT admet-389 88 25 wt wt NOUN admet-389 88 26 cells cell NOUN admet-389 88 27 is be AUX admet-389 88 28 summarized summarize VERB admet-389 88 29 in in ADP admet-389 88 30 figure figure NOUN admet-389 88 31 2 2 NUM admet-389 88 32 . . PUNCT admet-389 89 1 the the DET admet-389 89 2 three three NUM admet-389 89 3 compounds compound NOUN admet-389 89 4 accumulated accumulate VERB admet-389 89 5 in in ADP admet-389 89 6 the the DET admet-389 89 7 cells cell NOUN admet-389 89 8 in in ADP admet-389 89 9 a a DET admet-389 89 10 ph ph ADJ admet-389 89 11 - - ADJ admet-389 89 12 dependent dependent ADJ admet-389 89 13 manner manner NOUN admet-389 89 14 . . PUNCT admet-389 90 1 highest high ADJ admet-389 90 2 uptake uptake ADJ admet-389 90 3 was be AUX admet-389 90 4 observed observe VERB admet-389 90 5 at at ADP admet-389 90 6 alkaline alkaline ADJ admet-389 90 7 ph ph ADJ admet-389 90 8 range range NOUN admet-389 90 9 of of ADP admet-389 90 10 8.0 8.0 NUM admet-389 90 11 8.5 8.5 NUM admet-389 90 12 for for ADP admet-389 90 13 cho cho NOUN admet-389 90 14 cells cell NOUN admet-389 90 15 stably stably ADV admet-389 90 16 - - PUNCT admet-389 90 17 expressing express VERB admet-389 90 18 hoct2 hoct2 NOUN admet-389 90 19 . . PUNCT admet-389 91 1 conversely conversely ADV admet-389 91 2 , , PUNCT admet-389 91 3 decreased decrease VERB admet-389 91 4 uptake uptake ADJ admet-389 91 5 was be AUX admet-389 91 6 seen see VERB admet-389 91 7 in in ADP admet-389 91 8 acidic acidic ADJ admet-389 91 9 ph ph ADJ admet-389 91 10 range range NOUN admet-389 91 11 . . PUNCT admet-389 92 1 for for ADP admet-389 92 2 cho cho NOUN admet-389 92 3 - - PUNCT admet-389 92 4 wt wt NOUN admet-389 92 5 cells cell NOUN admet-389 92 6 , , PUNCT admet-389 92 7 no no DET admet-389 92 8 clear clear ADJ admet-389 92 9 pattern pattern NOUN admet-389 92 10 of of ADP admet-389 92 11 ph ph ADJ admet-389 92 12 - - PUNCT admet-389 92 13 dependency dependency NOUN admet-389 92 14 was be AUX admet-389 92 15 deciphered decipher VERB admet-389 92 16 . . PUNCT admet-389 93 1 the the DET admet-389 93 2 observed observe VERB admet-389 93 3 ph ph ADJ admet-389 93 4 effect effect NOUN admet-389 93 5 suggests suggest VERB admet-389 93 6 that that SCONJ admet-389 93 7 ph ph ADJ admet-389 93 8 - - ADJ admet-389 93 9 sensitive sensitive ADJ admet-389 93 10 organic organic ADJ admet-389 93 11 cation cation NOUN admet-389 93 12 transporters transporter NOUN admet-389 93 13 may may AUX admet-389 93 14 have have AUX admet-389 93 15 contributed contribute VERB admet-389 93 16 to to ADP admet-389 93 17 the the DET admet-389 93 18 observed observe VERB admet-389 93 19 solute solute NOUN admet-389 93 20 transport transport NOUN admet-389 93 21 as as ADP admet-389 93 22 no no DET admet-389 93 23 clear clear ADJ admet-389 93 24 ph ph ADJ admet-389 93 25 - - ADJ admet-389 93 26 dependent dependent ADJ admet-389 93 27 admet admet NOUN admet-389 93 28 & & CCONJ admet-389 93 29 dmpk dmpk PROPN admet-389 93 30 5(2 5(2 NUM admet-389 93 31 ) ) PUNCT admet-389 93 32 ( ( PUNCT admet-389 93 33 2017 2017 NUM admet-389 93 34 ) ) PUNCT admet-389 93 35 135 135 NUM admet-389 93 36 - - SYM admet-389 93 37 145 145 NUM admet-389 93 38 fluorescent fluorescent ADJ admet-389 93 39 organic organic ADJ admet-389 93 40 cations cation NOUN admet-389 93 41 for for ADP admet-389 93 42 human human ADJ admet-389 93 43 oct2 oct2 NOUN admet-389 93 44 transporters transporter NOUN admet-389 93 45 screening screen VERB admet-389 93 46 doi doi NOUN admet-389 93 47 : : PUNCT admet-389 93 48 10.5599 10.5599 NUM admet-389 93 49 / / SYM admet-389 93 50 admet.5.2.389 admet.5.2.389 NOUN admet-389 93 51 139 139 NUM admet-389 93 52 pattern pattern NOUN admet-389 93 53 was be AUX admet-389 93 54 seen see VERB admet-389 93 55 in in ADP admet-389 93 56 cho cho NOUN admet-389 93 57 - - PUNCT admet-389 93 58 wt wt NOUN admet-389 93 59 cells cell NOUN admet-389 93 60 . . PUNCT admet-389 94 1 the the DET admet-389 94 2 ph ph ADJ admet-389 94 3 effect effect NOUN admet-389 94 4 for for ADP admet-389 94 5 amiloride amiloride NOUN admet-389 94 6 was be AUX admet-389 94 7 comparable comparable ADJ admet-389 94 8 and and CCONJ admet-389 94 9 in in ADP admet-389 94 10 agreement agreement NOUN admet-389 94 11 with with ADP admet-389 94 12 published publish VERB admet-389 94 13 data datum NOUN admet-389 94 14 [ [ X admet-389 94 15 9,17,21 9,17,21 NUM admet-389 94 16 ] ] PUNCT admet-389 94 17 . . PUNCT admet-389 95 1 these these DET admet-389 95 2 studies study NOUN admet-389 95 3 involving involve VERB admet-389 95 4 the the DET admet-389 95 5 uptake uptake NOUN admet-389 95 6 of of ADP admet-389 95 7 4 4 NUM admet-389 95 8 - - PUNCT admet-389 95 9 di-1 di-1 NOUN admet-389 95 10 - - PUNCT admet-389 95 11 asp asp NOUN admet-389 95 12 by by ADP admet-389 95 13 octs oct NOUN admet-389 95 14 in in ADP admet-389 95 15 calu-3 calu-3 PROPN admet-389 95 16 , , PUNCT admet-389 95 17 a549 a549 PROPN admet-389 95 18 , , PUNCT admet-389 95 19 16hbe14oand 16hbe14oand NUM admet-389 95 20 caco-2 caco-2 NUM admet-389 95 21 cells cell NOUN admet-389 95 22 demonstrated demonstrate VERB admet-389 95 23 higher high ADJ admet-389 95 24 substrate substrate NOUN admet-389 95 25 accumulation accumulation NOUN admet-389 95 26 under under ADP admet-389 95 27 alkaline alkaline NOUN admet-389 95 28 extracellular extracellular ADJ admet-389 95 29 ph ph ADJ admet-389 95 30 range range NOUN admet-389 95 31 . . PUNCT admet-389 96 1 figure figure NOUN admet-389 96 2 2 2 NUM admet-389 96 3 . . PUNCT admet-389 96 4 effect effect NOUN admet-389 96 5 of of ADP admet-389 96 6 extracellular extracellular ADJ admet-389 96 7 ph ph NOUN admet-389 96 8 on on ADP admet-389 96 9 uptake uptake NOUN admet-389 96 10 in in ADP admet-389 96 11 cho cho NOUN admet-389 96 12 cells cell NOUN admet-389 96 13 stably stably ADV admet-389 96 14 expressing express VERB admet-389 96 15 hoct2 hoct2 NOUN admet-389 96 16 and and CCONJ admet-389 96 17 cho cho ADJ admet-389 96 18 wild wild ADJ admet-389 96 19 type type NOUN admet-389 96 20 . . PUNCT admet-389 97 1 uptake uptake NOUN admet-389 97 2 of of ADP admet-389 97 3 the the DET admet-389 97 4 compounds compound NOUN admet-389 97 5 ( ( PUNCT admet-389 97 6 10m 10m NUM admet-389 97 7 ) ) PUNCT admet-389 97 8 was be AUX admet-389 97 9 evaluated evaluate VERB admet-389 97 10 at at ADP admet-389 97 11 37 37 NUM admet-389 97 12 ° ° NOUN admet-389 97 13 c c NOUN admet-389 97 14 for for ADP admet-389 97 15 7 7 NUM admet-389 97 16 min min NOUN admet-389 97 17 using use VERB admet-389 97 18 cho cho PROPN admet-389 97 19 - - PUNCT admet-389 97 20 hoct2 hoct2 NOUN admet-389 97 21 , , PUNCT admet-389 97 22 and and CCONJ admet-389 97 23 cho cho ADJ admet-389 97 24 - - PUNCT admet-389 97 25 wt wt NOUN admet-389 97 26 cells cell NOUN admet-389 97 27 . . PUNCT admet-389 98 1 subsequently subsequently ADV admet-389 98 2 , , PUNCT admet-389 98 3 the the DET admet-389 98 4 cells cell NOUN admet-389 98 5 were be AUX admet-389 98 6 washed wash VERB admet-389 98 7 with with ADP admet-389 98 8 ice ice NOUN admet-389 98 9 - - PUNCT admet-389 98 10 cold cold ADJ admet-389 98 11 uptake uptake ADJ admet-389 98 12 buffer buffer NOUN admet-389 98 13 followed follow VERB admet-389 98 14 by by ADP admet-389 98 15 extraction extraction NOUN admet-389 98 16 with with ADP admet-389 98 17 triton triton PROPN admet-389 98 18 x-100/0.1 x-100/0.1 PROPN admet-389 98 19 m m PROPN admet-389 98 20 naoh naoh PROPN admet-389 98 21 . . PUNCT admet-389 99 1 data datum NOUN admet-389 99 2 represent represent VERB admet-389 99 3 mean mean PROPN admet-389 99 4 ± ± NUM admet-389 99 5 sd sd NOUN admet-389 99 6 ; ; PUNCT admet-389 99 7 n n NOUN admet-389 99 8 = = SYM admet-389 99 9 3 3 X admet-389 99 10 . . X admet-389 100 1 if if SCONJ admet-389 100 2 the the DET admet-389 100 3 uptake uptake NOUN admet-389 100 4 of of ADP admet-389 100 5 the the DET admet-389 100 6 fluorescent fluorescent ADJ admet-389 100 7 cations cation NOUN admet-389 100 8 was be AUX admet-389 100 9 mediated mediate VERB admet-389 100 10 by by ADP admet-389 100 11 hoct2 hoct2 NOUN admet-389 100 12 , , PUNCT admet-389 100 13 concentration concentration NOUN admet-389 100 14 - - PUNCT admet-389 100 15 dependent dependent ADJ admet-389 100 16 and and CCONJ admet-389 100 17 saturable saturable ADJ admet-389 100 18 uptake uptake NOUN admet-389 100 19 would would AUX admet-389 100 20 be be AUX admet-389 100 21 expected expect VERB admet-389 100 22 . . PUNCT admet-389 101 1 figure figure NOUN admet-389 101 2 3 3 NUM admet-389 101 3 shows show VERB admet-389 101 4 the the DET admet-389 101 5 effect effect NOUN admet-389 101 6 of of ADP admet-389 101 7 concentration concentration NOUN admet-389 101 8 on on ADP admet-389 101 9 amiloride amiloride NOUN admet-389 101 10 , , PUNCT admet-389 101 11 rhodamine rhodamine NOUN admet-389 101 12 123 123 NUM admet-389 101 13 , , PUNCT admet-389 101 14 and and CCONJ admet-389 101 15 rhodamine rhodamine ADJ admet-389 101 16 6 6 NUM admet-389 101 17 g g NOUN admet-389 101 18 uptake uptake ADJ admet-389 101 19 by by ADP admet-389 101 20 cho cho ADJ admet-389 101 21 - - PUNCT admet-389 101 22 hoct2 hoct2 PROPN admet-389 101 23 and and CCONJ admet-389 101 24 cho cho NOUN admet-389 101 25 - - PUNCT admet-389 101 26 wt wt NOUN admet-389 101 27 cells cell NOUN admet-389 101 28 . . PUNCT admet-389 102 1 a a DET admet-389 102 2 clear clear ADJ admet-389 102 3 concentration concentration NOUN admet-389 102 4 – – PUNCT admet-389 102 5 dependent dependent ADJ admet-389 102 6 effect effect NOUN admet-389 102 7 was be AUX admet-389 102 8 observed observe VERB admet-389 102 9 for for ADP admet-389 102 10 cho cho ADJ admet-389 102 11 - - PUNCT admet-389 102 12 hoct2 hoct2 NOUN admet-389 102 13 cells cell NOUN admet-389 102 14 . . PUNCT admet-389 103 1 at at ADP admet-389 103 2 various various ADJ admet-389 103 3 concentrations concentration NOUN admet-389 103 4 , , PUNCT admet-389 103 5 amiloride amiloride ADV admet-389 103 6 , , PUNCT admet-389 103 7 rhodamine rhodamine NOUN admet-389 103 8 6 6 NUM admet-389 103 9 g g NOUN admet-389 103 10 and and CCONJ admet-389 103 11 rhodamine rhodamine NOUN admet-389 103 12 123 123 NUM admet-389 103 13 were be AUX admet-389 103 14 accumulated accumulate VERB admet-389 103 15 to to ADP admet-389 103 16 a a DET admet-389 103 17 greater great ADJ admet-389 103 18 amount amount NOUN admet-389 103 19 by by ADP admet-389 103 20 cho cho ADJ admet-389 103 21 - - PUNCT admet-389 103 22 hoct2 hoct2 NOUN admet-389 103 23 cells cell NOUN admet-389 103 24 compared compare VERB admet-389 103 25 to to ADP admet-389 103 26 cho cho NOUN admet-389 103 27 - - PUNCT admet-389 103 28 wt wt NOUN admet-389 103 29 cells cell NOUN admet-389 103 30 . . PUNCT admet-389 104 1 uptake uptake ADJ admet-389 104 2 of of ADP admet-389 104 3 the the DET admet-389 104 4 compounds compound NOUN admet-389 104 5 in in ADP admet-389 104 6 cho cho ADJ admet-389 104 7 - - PUNCT admet-389 104 8 hoct2 hoct2 NOUN admet-389 104 9 cells cell NOUN admet-389 104 10 ( ( PUNCT admet-389 104 11 except except SCONJ admet-389 104 12 rhodamine rhodamine NOUN admet-389 104 13 123 123 NUM admet-389 104 14 ) ) PUNCT admet-389 104 15 was be AUX admet-389 104 16 saturable saturable ADJ admet-389 104 17 . . PUNCT admet-389 105 1 the the DET admet-389 105 2 calculated calculate VERB admet-389 105 3 kinetic kinetic ADJ admet-389 105 4 parameters parameter NOUN admet-389 105 5 are be AUX admet-389 105 6 : : PUNCT admet-389 105 7 km km NOUN admet-389 105 8 = = SYM admet-389 105 9 72.6312.02 72.6312.02 NUM admet-389 105 10 µm µm NOUN admet-389 105 11 , , PUNCT admet-389 105 12 vmax vmax PROPN admet-389 105 13 = = PUNCT admet-389 105 14 0.0270.001 0.0270.001 NUM admet-389 105 15 µm µm ADP admet-389 105 16 / / SYM admet-389 105 17 mg mg PROPN admet-389 105 18 protein protein NOUN admet-389 105 19 / / SYM admet-389 105 20 min min NOUN admet-389 105 21 ( ( PUNCT admet-389 105 22 amiloride amiloride ADV admet-389 105 23 ) ) PUNCT admet-389 105 24 and and CCONJ admet-389 105 25 km km NOUN admet-389 105 26 = = SYM admet-389 105 27 82.4729.15 82.4729.15 NUM admet-389 105 28 µm µm NOUN admet-389 105 29 , , PUNCT admet-389 105 30 vmax= vmax= NUM admet-389 105 31 0.0190.002 0.0190.002 NOUN admet-389 105 32 µm µm ADP admet-389 105 33 / / SYM admet-389 105 34 mg mg PROPN admet-389 105 35 protein protein NOUN admet-389 105 36 / / SYM admet-389 105 37 min min PROPN admet-389 105 38 ( ( PUNCT admet-389 105 39 rhodamine rhodamine NOUN admet-389 105 40 6 6 NUM admet-389 105 41 g g NOUN admet-389 105 42 ) ) PUNCT admet-389 105 43 , , PUNCT admet-389 105 44 respectively respectively ADV admet-389 105 45 . . PUNCT admet-389 106 1 we we PRON admet-389 106 2 were be AUX admet-389 106 3 unable unable ADJ admet-389 106 4 to to PART admet-389 106 5 determine determine VERB admet-389 106 6 the the DET admet-389 106 7 kinetic kinetic ADJ admet-389 106 8 parameters parameter NOUN admet-389 106 9 for for ADP admet-389 106 10 rhodamine rhodamine NOUN admet-389 106 11 123 123 NUM admet-389 106 12 due due ADJ admet-389 106 13 to to ADP admet-389 106 14 excessive excessive ADJ admet-389 106 15 non non ADJ admet-389 106 16 - - ADJ admet-389 106 17 specific specific ADJ admet-389 106 18 binding binding NOUN admet-389 106 19 to to ADP admet-389 106 20 the the DET admet-389 106 21 96 96 NUM admet-389 106 22 - - PUNCT admet-389 106 23 well well NOUN admet-389 106 24 plates plate NOUN admet-389 106 25 at at ADP admet-389 106 26 higher high ADJ admet-389 106 27 concentrations concentration NOUN admet-389 106 28 . . PUNCT admet-389 107 1 these these DET admet-389 107 2 results result NOUN admet-389 107 3 indicate indicate VERB admet-389 107 4 that that DET admet-389 107 5 amiloride amiloride NOUN admet-389 107 6 and and CCONJ admet-389 107 7 rhodamine rhodamine ADJ admet-389 107 8 6 6 NUM admet-389 107 9 g g NOUN admet-389 107 10 appear appear VERB admet-389 107 11 to to PART admet-389 107 12 be be AUX admet-389 107 13 better well ADJ admet-389 107 14 substrates substrate NOUN admet-389 107 15 for for ADP admet-389 107 16 hoct2 hoct2 NOUN admet-389 107 17 compared compare VERB admet-389 107 18 to to ADP admet-389 107 19 rhodamine rhodamine NOUN admet-389 107 20 123 123 NUM admet-389 107 21 . . PUNCT admet-389 108 1 the the DET admet-389 108 2 rank rank NOUN admet-389 108 3 order order NOUN admet-389 108 4 of of ADP admet-389 108 5 affinity affinity NOUN admet-389 108 6 of of ADP admet-389 108 7 the the DET admet-389 108 8 dyes dye NOUN admet-389 108 9 based base VERB admet-389 108 10 on on ADP admet-389 108 11 michaelis michaeli NOUN admet-389 108 12 - - PUNCT admet-389 108 13 menten menten ADJ admet-389 108 14 modelling modelling NOUN admet-389 108 15 is be AUX admet-389 108 16 : : PUNCT admet-389 108 17 amiloride amiloride ADJ admet-389 108 18 ( ( PUNCT admet-389 108 19 km km NOUN admet-389 108 20 = = SYM admet-389 108 21 72.63 72.63 NUM admet-389 108 22   PROPN admet-389 108 23 12.02 12.02 NUM admet-389 108 24 µm µm NOUN admet-389 108 25 ) ) PUNCT admet-389 108 26 > > X admet-389 109 1 rhodamine rhodamine ADJ admet-389 109 2 6 6 NUM admet-389 109 3 g g NOUN admet-389 109 4 ( ( PUNCT admet-389 109 5 km km NOUN admet-389 109 6 = = SYM admet-389 109 7 82.47 82.47 NUM admet-389 109 8   PROPN admet-389 109 9 29.15 29.15 NUM admet-389 109 10 µm µm NOUN admet-389 109 11 ) ) PUNCT admet-389 109 12 > > X admet-389 109 13 rhodamine rhodamine NOUN admet-389 109 14 123 123 NUM admet-389 109 15 ( ( PUNCT admet-389 109 16 km km NOUN admet-389 109 17 = = PUNCT admet-389 109 18 nd nd NUM admet-389 109 19 ) ) PUNCT admet-389 109 20 . . PUNCT admet-389 110 1 as as SCONJ admet-389 110 2 km km PROPN admet-389 110 3 is be AUX admet-389 110 4 a a DET admet-389 110 5 measure measure NOUN admet-389 110 6 of of ADP admet-389 110 7 transporter transporter NOUN admet-389 110 8 - - PUNCT admet-389 110 9 substrate substrate NOUN admet-389 110 10 affinity affinity NOUN admet-389 110 11 [ [ X admet-389 110 12 22 22 NUM admet-389 110 13 - - SYM admet-389 110 14 23 23 NUM admet-389 110 15 ] ] PUNCT admet-389 110 16 , , PUNCT admet-389 110 17 higher high ADJ admet-389 110 18 km km NOUN admet-389 110 19 implies imply VERB admet-389 110 20 poorer poor ADJ admet-389 110 21 binding binding ADJ admet-389 110 22 / / SYM admet-389 110 23 affinity affinity NOUN admet-389 110 24 . . PUNCT admet-389 111 1 biermann biermann PROPN admet-389 111 2 et et PROPN admet-389 111 3 al al PROPN admet-389 111 4 . . PROPN admet-389 111 5 , , PUNCT admet-389 112 1 [ [ X admet-389 112 2 2 2 X admet-389 112 3 ] ] PUNCT admet-389 112 4 used use VERB admet-389 112 5 km km NOUN admet-389 112 6 to to PART admet-389 112 7 compare compare VERB admet-389 112 8 the the DET admet-389 112 9 affinities affinity NOUN admet-389 112 10 of of ADP admet-389 112 11 different different ADJ admet-389 112 12 fluorescent fluorescent NOUN admet-389 112 13 compounds compound NOUN admet-389 112 14 to to ADP admet-389 112 15 hoct2 hoct2 NOUN admet-389 112 16 in in ADP admet-389 112 17 transfected transfecte VERB admet-389 112 18 hek-293 hek-293 NOUN admet-389 112 19 . . PUNCT admet-389 113 1 according accord VERB admet-389 113 2 to to ADP admet-389 113 3 the the DET admet-389 113 4 authors author NOUN admet-389 113 5 , , PUNCT admet-389 113 6 amiloride amiloride NOUN admet-389 113 7 and and CCONJ admet-389 113 8 4 4 NUM admet-389 113 9 - - PUNCT admet-389 113 10 di-1 di-1 PRON admet-389 113 11 - - PUNCT admet-389 113 12 asp asp NOUN admet-389 113 13 had have VERB admet-389 113 14 significantly significantly ADV admet-389 113 15 higher high ADJ admet-389 113 16 uptake uptake ADJ admet-389 113 17 rates rate NOUN admet-389 113 18 in in ADP admet-389 113 19 hoct2 hoct2 NOUN admet-389 113 20 cells cell NOUN admet-389 113 21 compared compare VERB admet-389 113 22 to to ADP admet-389 113 23 wild wild ADJ admet-389 113 24 type type NOUN admet-389 113 25 cells cell NOUN admet-389 113 26 . . PUNCT admet-389 114 1 the the DET admet-389 114 2 km km NOUN admet-389 114 3 obtained obtain VERB admet-389 114 4 in in ADP admet-389 114 5 this this DET admet-389 114 6 study study NOUN admet-389 114 7 was be AUX admet-389 114 8 remigius remigius PROPN admet-389 114 9 agu agu NOUN admet-389 114 10 et et PROPN admet-389 114 11 al al PROPN admet-389 114 12 . . PROPN admet-389 114 13 admet admet PROPN admet-389 114 14 & & CCONJ admet-389 114 15 dmpk dmpk PROPN admet-389 114 16 5(2 5(2 NUM admet-389 114 17 ) ) PUNCT admet-389 114 18 ( ( PUNCT admet-389 114 19 2017 2017 NUM admet-389 114 20 ) ) PUNCT admet-389 114 21 135 135 NUM admet-389 114 22 - - SYM admet-389 114 23 145 145 NUM admet-389 114 24 140 140 NUM admet-389 114 25 comparable comparable ADJ admet-389 114 26 to to ADP admet-389 114 27 the the DET admet-389 114 28 value value NOUN admet-389 114 29 ( ( PUNCT admet-389 114 30 95 95 NUM admet-389 114 31 µm µm NOUN admet-389 114 32 ) ) PUNCT admet-389 114 33 obtained obtain VERB admet-389 114 34 by by ADP admet-389 114 35 biermann biermann PROPN admet-389 114 36 et et PROPN admet-389 114 37 al al PROPN admet-389 114 38 using use VERB admet-389 114 39 hek-293 hek-293 NOUN admet-389 114 40 stably stably ADV admet-389 114 41 expressing express VERB admet-389 114 42 hoct2 hoct2 NOUN admet-389 114 43 . . PUNCT admet-389 115 1 amiloride amiloride PROPN admet-389 115 2 is be AUX admet-389 115 3 not not PART admet-389 115 4 only only ADV admet-389 115 5 taken take VERB admet-389 115 6 up up ADP admet-389 115 7 by by ADP admet-389 115 8 cells cell NOUN admet-389 115 9 , , PUNCT admet-389 115 10 but but CCONJ admet-389 115 11 can can AUX admet-389 115 12 be be AUX admet-389 115 13 transported transport VERB admet-389 115 14 . . PUNCT admet-389 116 1 this this DET admet-389 116 2 behaviour behaviour NOUN admet-389 116 3 may may AUX admet-389 116 4 be be AUX admet-389 116 5 significantly significantly ADV admet-389 116 6 affected affect VERB admet-389 116 7 by by ADP admet-389 116 8 the the DET admet-389 116 9 degree degree NOUN admet-389 116 10 of of ADP admet-389 116 11 ionization ionization NOUN admet-389 116 12 of of ADP admet-389 116 13 the the DET admet-389 116 14 compound compound NOUN admet-389 116 15 . . PUNCT admet-389 117 1 the the DET admet-389 117 2 guanidine guanidine ADJ admet-389 117 3 moiety moiety NOUN admet-389 117 4 of of ADP admet-389 117 5 the the DET admet-389 117 6 compound compound NOUN admet-389 117 7 is be AUX admet-389 117 8 protonated protonate VERB admet-389 117 9 at at ADP admet-389 117 10 physiologic physiologic ADJ admet-389 117 11 ph ph VERB admet-389 117 12 , , PUNCT admet-389 117 13 and and CCONJ admet-389 117 14 it it PRON admet-389 117 15 is be AUX admet-389 117 16 this this DET admet-389 117 17 positively positively ADV admet-389 117 18 charged charge VERB admet-389 117 19 species specie NOUN admet-389 117 20 that that PRON admet-389 117 21 makes make VERB admet-389 117 22 it it PRON admet-389 117 23 an an DET admet-389 117 24 organic organic ADJ admet-389 117 25 cation cation NOUN admet-389 117 26 [ [ X admet-389 117 27 24 24 NUM admet-389 117 28 ] ] PUNCT admet-389 117 29 . . PUNCT admet-389 118 1 as as ADP admet-389 118 2 a a DET admet-389 118 3 weak weak ADJ admet-389 118 4 base base NOUN admet-389 118 5 , , PUNCT admet-389 118 6 the the DET admet-389 118 7 degree degree NOUN admet-389 118 8 of of ADP admet-389 118 9 ionization ionization NOUN admet-389 118 10 plays play VERB admet-389 118 11 a a DET admet-389 118 12 critical critical ADJ admet-389 118 13 role role NOUN admet-389 118 14 in in ADP admet-389 118 15 its its PRON admet-389 118 16 binding binding NOUN admet-389 118 17 to to ADP admet-389 118 18 organic organic ADJ admet-389 118 19 cation cation NOUN admet-389 118 20 transporters transporter NOUN admet-389 118 21 [ [ X admet-389 118 22 25 25 NUM admet-389 118 23 ] ] PUNCT admet-389 118 24 . . PUNCT admet-389 119 1 this this PRON admet-389 119 2 implies imply VERB admet-389 119 3 that that DET admet-389 119 4 permeation permeation NOUN admet-389 119 5 at at ADP admet-389 119 6 ph ph NOUN admet-389 119 7 much much ADV admet-389 119 8 lower low ADJ admet-389 119 9 than than ADP admet-389 119 10 the the DET admet-389 119 11 pka pka NOUN admet-389 119 12 of of ADP admet-389 119 13 the the DET admet-389 119 14 compound compound NOUN admet-389 119 15 may may AUX admet-389 119 16 be be AUX admet-389 119 17 by by ADP admet-389 119 18 passive passive ADJ admet-389 119 19 diffusion diffusion NOUN admet-389 119 20 . . PUNCT admet-389 120 1 for for SCONJ admet-389 120 2 the the DET admet-389 120 3 cationic cationic ADJ admet-389 120 4 rhodamines rhodamine NOUN admet-389 120 5 investigated investigate VERB admet-389 120 6 , , PUNCT admet-389 120 7 there there PRON admet-389 120 8 was be VERB admet-389 120 9 no no DET admet-389 120 10 clear clear ADV admet-389 120 11 - - PUNCT admet-389 120 12 cut cut VERB admet-389 120 13 ph ph ADJ admet-389 120 14 effect effect NOUN admet-389 120 15 within within ADP admet-389 120 16 the the DET admet-389 120 17 range range NOUN admet-389 120 18 investigated investigate VERB admet-389 120 19 . . PUNCT admet-389 121 1 this this PRON admet-389 121 2 was be AUX admet-389 121 3 not not PART admet-389 121 4 surprising surprising ADJ admet-389 121 5 because because SCONJ admet-389 121 6 we we PRON admet-389 121 7 previously previously ADV admet-389 121 8 showed show VERB admet-389 121 9 that that SCONJ admet-389 121 10 fluorescence fluorescence NOUN admet-389 121 11 intensity intensity NOUN admet-389 121 12 of of ADP admet-389 121 13 rhodamine-6 rhodamine-6 PROPN admet-389 121 14 g g PROPN admet-389 121 15 is be AUX admet-389 121 16 characteristically characteristically ADV admet-389 121 17 acid acid ADJ admet-389 121 18 - - PUNCT admet-389 121 19 dependent dependent ADJ admet-389 121 20 due due ADP admet-389 121 21 to to ADP admet-389 121 22 molecular molecular ADJ admet-389 121 23 changes change NOUN admet-389 121 24 and and CCONJ admet-389 121 25 transitions transition NOUN admet-389 121 26 [ [ X admet-389 121 27 17 17 NUM admet-389 121 28 ] ] PUNCT admet-389 121 29 . . PUNCT admet-389 122 1 under under ADP admet-389 122 2 our our PRON admet-389 122 3 experimental experimental ADJ admet-389 122 4 conditions condition NOUN admet-389 122 5 , , PUNCT admet-389 122 6 it it PRON admet-389 122 7 was be AUX admet-389 122 8 not not PART admet-389 122 9 possible possible ADJ admet-389 122 10 to to PART admet-389 122 11 differentiate differentiate VERB admet-389 122 12 the the DET admet-389 122 13 effect effect NOUN admet-389 122 14 of of ADP admet-389 122 15 this this DET admet-389 122 16 phenomenon phenomenon NOUN admet-389 122 17 on on ADP admet-389 122 18 uptake uptake NOUN admet-389 122 19 from from ADP admet-389 122 20 the the DET admet-389 122 21 impact impact NOUN admet-389 122 22 of of ADP admet-389 122 23 ph ph ADJ admet-389 122 24 changes change NOUN admet-389 122 25 on on ADP admet-389 122 26 uptake uptake ADJ admet-389 122 27 . . PUNCT admet-389 123 1 figure figure VERB admet-389 123 2 3 3 NUM admet-389 123 3 . . PUNCT admet-389 123 4 concentration concentration NOUN admet-389 123 5 - - PUNCT admet-389 123 6 dependent dependent ADJ admet-389 123 7 intracellular intracellular ADJ admet-389 123 8 uptake uptake NOUN admet-389 123 9 of of ADP admet-389 123 10 the the DET admet-389 123 11 fluorescent fluorescent NOUN admet-389 123 12 compounds compound NOUN admet-389 123 13 in in ADP admet-389 123 14 cho cho ADJ admet-389 123 15 - - PUNCT admet-389 123 16 hoct2 hoct2 PROPN admet-389 123 17 and and CCONJ admet-389 123 18 cho cho NOUN admet-389 123 19 - - PUNCT admet-389 123 20 wt wt NOUN admet-389 123 21 . . NOUN admet-389 123 22 uptake uptake NOUN admet-389 123 23 was be AUX admet-389 123 24 evaluated evaluate VERB admet-389 123 25 at at ADP admet-389 123 26 37 37 NUM admet-389 123 27 ° ° NOUN admet-389 123 28 c c NOUN admet-389 123 29 for for ADP admet-389 123 30 7 7 NUM admet-389 123 31 min min NOUN admet-389 123 32 . . PROPN admet-389 123 33 subsequently subsequently ADV admet-389 123 34 , , PUNCT admet-389 123 35 the the DET admet-389 123 36 cells cell NOUN admet-389 123 37 were be AUX admet-389 123 38 washed wash VERB admet-389 123 39 with with ADP admet-389 123 40 ice ice NOUN admet-389 123 41 - - PUNCT admet-389 123 42 cold cold ADJ admet-389 123 43 uptake uptake ADJ admet-389 123 44 buffer buffer NOUN admet-389 123 45 followed follow VERB admet-389 123 46 by by ADP admet-389 123 47 extraction extraction NOUN admet-389 123 48 with with ADP admet-389 123 49 triton triton PROPN admet-389 123 50 x-100/0.1 x-100/0.1 PROPN admet-389 123 51 m m PROPN admet-389 123 52 naoh naoh PROPN admet-389 123 53 . . PUNCT admet-389 124 1 data datum NOUN admet-389 124 2 represent represent VERB admet-389 124 3 mean mean PROPN admet-389 124 4 ± ± NUM admet-389 124 5 sd sd NOUN admet-389 124 6 ; ; PUNCT admet-389 124 7 n n NOUN admet-389 124 8 = = SYM admet-389 124 9 3 3 X admet-389 124 10 . . X admet-389 125 1 if if SCONJ admet-389 125 2 the the DET admet-389 125 3 uptake uptake NOUN admet-389 125 4 of of ADP admet-389 125 5 the the DET admet-389 125 6 cationic cationic ADJ admet-389 125 7 fluorochromes fluorochrome NOUN admet-389 125 8 were be AUX admet-389 125 9 mediated mediate VERB admet-389 125 10 by by ADP admet-389 125 11 hoct2 hoct2 NOUN admet-389 125 12 , , PUNCT admet-389 125 13 one one PRON admet-389 125 14 would would AUX admet-389 125 15 expect expect VERB admet-389 125 16 hoct2 hoct2 NOUN admet-389 125 17 inhibitors inhibitor NOUN admet-389 125 18 to to PART admet-389 125 19 differentially differentially ADV admet-389 125 20 reduce reduce VERB admet-389 125 21 the the DET admet-389 125 22 uptake uptake NOUN admet-389 125 23 of of ADP admet-389 125 24 these these DET admet-389 125 25 compounds compound NOUN admet-389 125 26 in in ADP admet-389 125 27 transfected transfecte VERB admet-389 125 28 cells cell NOUN admet-389 125 29 ( ( PUNCT admet-389 125 30 figures figure NOUN admet-389 125 31 4 4 NUM admet-389 125 32 - - SYM admet-389 125 33 7 7 NUM admet-389 125 34 ) ) PUNCT admet-389 125 35 . . PUNCT admet-389 126 1 we we PRON admet-389 126 2 used use VERB admet-389 126 3 six six NUM admet-389 126 4 inhibitors inhibitor NOUN admet-389 126 5 ( ( PUNCT admet-389 126 6 figure figure NOUN admet-389 126 7 4 4 NUM admet-389 126 8 ) ) PUNCT admet-389 126 9 at at ADP admet-389 126 10 concentration concentration NOUN admet-389 126 11 of of ADP admet-389 126 12 1 1 NUM admet-389 126 13 mm mm NOUN admet-389 126 14 for for ADP admet-389 126 15 initial initial ADJ admet-389 126 16 screening screening NOUN admet-389 126 17 after after ADP admet-389 126 18 which which PRON admet-389 126 19 the the DET admet-389 126 20 three three NUM admet-389 126 21 most most ADV admet-389 126 22 potent potent ADJ admet-389 126 23 ( ( PUNCT admet-389 126 24 quinine quinine NOUN admet-389 126 25 , , PUNCT admet-389 126 26 verapamil verapamil NOUN admet-389 126 27 and and CCONJ admet-389 126 28 quinidine quinidine PROPN admet-389 126 29 hcl hcl PROPN admet-389 126 30 ) ) PUNCT admet-389 126 31 were be AUX admet-389 126 32 selected select VERB admet-389 126 33 for for ADP admet-389 126 34 a a DET admet-389 126 35 concentration concentration NOUN admet-389 126 36 dependent dependent ADJ admet-389 126 37 inhibition inhibition NOUN admet-389 126 38 studies study NOUN admet-389 126 39 ( ( PUNCT admet-389 126 40 figure figure NOUN admet-389 126 41 5 5 NUM admet-389 126 42 ) ) PUNCT admet-389 126 43 . . PUNCT admet-389 127 1 these these DET admet-389 127 2 inhibitors inhibitor NOUN admet-389 127 3 have have AUX admet-389 127 4 been be AUX admet-389 127 5 reported report VERB admet-389 127 6 by by ADP admet-389 127 7 other other ADJ admet-389 127 8 published publish VERB admet-389 127 9 literature literature NOUN admet-389 127 10 as as ADP admet-389 127 11 potent potent ADJ admet-389 127 12 and and CCONJ admet-389 127 13 oct oct NOUN admet-389 127 14 specific specific ADJ admet-389 127 15 inhibitors inhibitor NOUN admet-389 127 16 [ [ X admet-389 127 17 9 9 NUM admet-389 127 18 , , PUNCT admet-389 127 19 10 10 NUM admet-389 127 20 , , PUNCT admet-389 127 21 17 17 NUM admet-389 127 22 ] ] PUNCT admet-389 127 23 . . PUNCT admet-389 128 1 figure figure NOUN admet-389 128 2 4 4 NUM admet-389 128 3 shows show VERB admet-389 128 4 the the DET admet-389 128 5 effect effect NOUN admet-389 128 6 of of ADP admet-389 128 7 hoct2 hoct2 NOUN admet-389 128 8 and and CCONJ admet-389 128 9 octn1 octn1 ADJ admet-389 128 10 - - SYM admet-389 128 11 2 2 NUM admet-389 128 12 inhibitors inhibitor NOUN admet-389 128 13 and and CCONJ admet-389 128 14 substrates substrate NOUN admet-389 128 15 on on ADP admet-389 128 16 amiloride amiloride NOUN admet-389 128 17 , , PUNCT admet-389 128 18 rhodamine rhodamine NOUN admet-389 128 19 6 6 NUM admet-389 128 20 g g NOUN admet-389 128 21 and and CCONJ admet-389 128 22 rhodamine rhodamine NOUN admet-389 128 23 123 123 NUM admet-389 128 24 uptake uptake NOUN admet-389 128 25 in in ADP admet-389 128 26 cho cho ADJ admet-389 128 27 - - PUNCT admet-389 128 28 hoct2 hoct2 NOUN admet-389 128 29 cells cell NOUN admet-389 128 30 . . PUNCT admet-389 129 1 at at ADP admet-389 129 2 1 1 NUM admet-389 129 3 mm mm NOUN admet-389 129 4 , , PUNCT admet-389 129 5 quinidine quinidine ADJ admet-389 129 6 , , PUNCT admet-389 129 7 quinine quinine NOUN admet-389 129 8 and and CCONJ admet-389 129 9 verapamil verapamil NOUN admet-389 129 10 consistently consistently ADV admet-389 129 11 inhibited inhibit VERB admet-389 129 12 amiloride amiloride NOUN admet-389 129 13 , , PUNCT admet-389 129 14 and and CCONJ admet-389 129 15 rhodamine rhodamine ADJ admet-389 129 16 6 6 NUM admet-389 129 17 g g NOUN admet-389 129 18 uptake uptake NOUN admet-389 129 19 . . PUNCT admet-389 130 1 expectedly expectedly ADV admet-389 130 2 , , PUNCT admet-389 130 3 octn octn ADJ admet-389 130 4 inhibitors inhibitor NOUN admet-389 130 5 ( ( PUNCT admet-389 130 6 nmn nmn X admet-389 130 7 , , PUNCT admet-389 130 8 choline choline NOUN admet-389 130 9 and and CCONJ admet-389 130 10 guanidine guanidine NOUN admet-389 130 11 ) ) PUNCT admet-389 130 12 had have VERB admet-389 130 13 no no DET admet-389 130 14 significant significant ADJ admet-389 130 15 effect effect NOUN admet-389 130 16 on on ADP admet-389 130 17 the the DET admet-389 130 18 uptake uptake NOUN admet-389 130 19 of of ADP admet-389 130 20 these these DET admet-389 130 21 compounds compound NOUN admet-389 130 22 ( ( PUNCT admet-389 130 23 p0.5 p0.5 PROPN admet-389 130 24 ) ) PUNCT admet-389 130 25 , , PUNCT admet-389 130 26 an an DET admet-389 130 27 indication indication NOUN admet-389 130 28 that that SCONJ admet-389 130 29 their their PRON admet-389 130 30 uptake uptake NOUN admet-389 130 31 was be AUX admet-389 130 32 likely likely ADJ admet-389 130 33 due due ADJ admet-389 130 34 to to ADP admet-389 130 35 hoct2 hoct2 NOUN admet-389 130 36 transporters transporter NOUN admet-389 130 37 . . PUNCT admet-389 131 1 to to PART admet-389 131 2 confirm confirm VERB admet-389 131 3 the the DET admet-389 131 4 affinity affinity NOUN admet-389 131 5 data datum NOUN admet-389 131 6 obtained obtain VERB admet-389 131 7 with with ADP admet-389 131 8 michaelis michaeli NOUN admet-389 131 9 - - PUNCT admet-389 131 10 menten menten ADJ admet-389 131 11 kinetics kinetic NOUN admet-389 131 12 , , PUNCT admet-389 131 13 concentration concentration NOUN admet-389 131 14 - - PUNCT admet-389 131 15 dependent dependent ADJ admet-389 131 16 inhibition inhibition NOUN admet-389 131 17 of of ADP admet-389 131 18 amiloride amiloride NOUN admet-389 131 19 , , PUNCT admet-389 131 20 rhodamine rhodamine NOUN admet-389 131 21 6 6 NUM admet-389 131 22 g g NOUN admet-389 131 23 , , PUNCT admet-389 131 24 and and CCONJ admet-389 131 25 rhodamine rhodamine NOUN admet-389 131 26 123 123 NUM admet-389 131 27 using use VERB admet-389 131 28 hoct2 hoct2 NOUN admet-389 131 29 inhibitors inhibitor NOUN admet-389 131 30 ( ( PUNCT admet-389 131 31 quinine quinine ADJ admet-389 131 32 , , PUNCT admet-389 131 33 quinidine quinidine ADJ admet-389 131 34 , , PUNCT admet-389 131 35 and and CCONJ admet-389 131 36 verapamil verapamil NOUN admet-389 131 37 ) ) PUNCT admet-389 131 38 were be AUX admet-389 131 39 investigated investigate VERB admet-389 131 40 ( ( PUNCT admet-389 131 41 figures figure NOUN admet-389 131 42 5 5 NUM admet-389 131 43 - - SYM admet-389 131 44 7 7 NUM admet-389 131 45 ) ) PUNCT admet-389 131 46 . . PUNCT admet-389 132 1 figure figure NOUN admet-389 132 2 5 5 NUM admet-389 132 3 shows show VERB admet-389 132 4 the the DET admet-389 132 5 effect effect NOUN admet-389 132 6 of of ADP admet-389 132 7 increasing increase VERB admet-389 132 8 concentrations concentration NOUN admet-389 132 9 of of ADP admet-389 132 10 quinidine quinidine NOUN admet-389 132 11 on on ADP admet-389 132 12 the the DET admet-389 132 13 fluorescent fluorescent NOUN admet-389 132 14 compounds compound VERB admet-389 132 15 uptake uptake ADJ admet-389 132 16 . . PUNCT admet-389 133 1 admet admet PROPN admet-389 133 2 & & CCONJ admet-389 133 3 dmpk dmpk PROPN admet-389 133 4 5(2 5(2 NUM admet-389 133 5 ) ) PUNCT admet-389 133 6 ( ( PUNCT admet-389 133 7 2017 2017 NUM admet-389 133 8 ) ) PUNCT admet-389 133 9 135 135 NUM admet-389 133 10 - - SYM admet-389 133 11 145 145 NUM admet-389 133 12 fluorescent fluorescent ADJ admet-389 133 13 organic organic ADJ admet-389 133 14 cations cation NOUN admet-389 133 15 for for ADP admet-389 133 16 human human ADJ admet-389 133 17 oct2 oct2 NOUN admet-389 133 18 transporters transporter NOUN admet-389 133 19 screening screen VERB admet-389 133 20 doi doi NOUN admet-389 133 21 : : PUNCT admet-389 133 22 10.5599 10.5599 NUM admet-389 133 23 / / SYM admet-389 133 24 admet.5.2.389 admet.5.2.389 NOUN admet-389 133 25 141 141 NUM admet-389 133 26 the the DET admet-389 133 27 ic50s ic50 NOUN admet-389 133 28 for for ADP admet-389 133 29 the the DET admet-389 133 30 inhibition inhibition NOUN admet-389 133 31 were be AUX admet-389 133 32 891 891 NUM admet-389 133 33 µm µm ADP admet-389 133 34 ( ( PUNCT admet-389 133 35 amiloride amiloride NOUN admet-389 133 36 ) ) PUNCT admet-389 133 37 , , PUNCT admet-389 133 38 315 315 NUM admet-389 133 39 µm µm NOUN admet-389 133 40 ( ( PUNCT admet-389 133 41 rhodamine rhodamine NOUN admet-389 133 42 123 123 NUM admet-389 133 43 ) ) PUNCT admet-389 133 44 and and CCONJ admet-389 133 45 1137 1137 NUM admet-389 133 46 µm µm ADP admet-389 133 47 ( ( PUNCT admet-389 133 48 rhodamine rhodamine PROPN admet-389 133 49 6 6 NUM admet-389 133 50 g g NOUN admet-389 133 51 ) ) PUNCT admet-389 133 52 . . PUNCT admet-389 134 1 similarly similarly ADV admet-389 134 2 , , PUNCT admet-389 134 3 figure figure VERB admet-389 134 4 6 6 NUM admet-389 134 5 summarizes summarize NOUN admet-389 134 6 concentration concentration NOUN admet-389 134 7 - - PUNCT admet-389 134 8 dependent dependent ADJ admet-389 134 9 inhibition inhibition NOUN admet-389 134 10 of of ADP admet-389 134 11 the the DET admet-389 134 12 cationic cationic ADJ admet-389 134 13 dyes dye NOUN admet-389 134 14 by by ADP admet-389 134 15 quinine quinine NOUN admet-389 134 16 . . PUNCT admet-389 135 1 the the DET admet-389 135 2 ic50s ic50 NOUN admet-389 135 3 for for ADP admet-389 135 4 the the DET admet-389 135 5 inhibition inhibition NOUN admet-389 135 6 were be AUX admet-389 135 7 309.6 309.6 NUM admet-389 135 8 , , PUNCT admet-389 135 9 279 279 NUM admet-389 135 10 , , PUNCT admet-389 135 11 and and CCONJ admet-389 135 12 206 206 NUM admet-389 135 13 µm µm NOUN admet-389 135 14 for for ADP admet-389 135 15 amiloride amiloride NOUN admet-389 135 16 , , PUNCT admet-389 135 17 rhodamine rhodamine NOUN admet-389 135 18 123 123 NUM admet-389 135 19 and and CCONJ admet-389 135 20 rhodamine rhodamine ADJ admet-389 135 21 6 6 NUM admet-389 135 22 g g NOUN admet-389 135 23 , , PUNCT admet-389 135 24 respectively respectively ADV admet-389 135 25 . . PUNCT admet-389 136 1 data datum NOUN admet-389 136 2 for for ADP admet-389 136 3 inhibition inhibition NOUN admet-389 136 4 studies study NOUN admet-389 136 5 with with ADP admet-389 136 6 verapamil verapamil NOUN admet-389 136 7 is be AUX admet-389 136 8 shown show VERB admet-389 136 9 in in ADP admet-389 136 10 figure figure NOUN admet-389 136 11 7 7 NUM admet-389 136 12 . . PUNCT admet-389 137 1 verapamil verapamil NOUN admet-389 137 2 concentrationdependently concentrationdependently ADV admet-389 137 3 inhibited inhibit VERB admet-389 137 4 amiloride amiloride ADV admet-389 137 5 ( ( PUNCT admet-389 137 6 ic50 ic50 PROPN admet-389 137 7 = = PROPN admet-389 137 8 486 486 NUM admet-389 137 9 µm µm NOUN admet-389 137 10 ) ) PUNCT admet-389 137 11 , , PUNCT admet-389 137 12 rhodamine rhodamine NOUN admet-389 137 13 123 123 NUM admet-389 137 14 ( ( PUNCT admet-389 137 15 ic50 ic50 PROPN admet-389 137 16 = = PROPN admet-389 137 17 302 302 NUM admet-389 137 18 µm µm NOUN admet-389 137 19 ) ) PUNCT admet-389 137 20 and and CCONJ admet-389 137 21 rhodamine rhodamine ADJ admet-389 137 22 6 6 NUM admet-389 137 23 g g NOUN admet-389 137 24 ( ( PUNCT admet-389 137 25 ic50 ic50 PROPN admet-389 137 26 = = PROPN admet-389 137 27 583 583 NUM admet-389 137 28 µm µm NOUN admet-389 137 29 ) ) PUNCT admet-389 137 30 . . PUNCT admet-389 138 1 inhibition inhibition NOUN admet-389 138 2 of of ADP admet-389 138 3 the the DET admet-389 138 4 cationic cationic ADJ admet-389 138 5 compounds compound NOUN admet-389 138 6 by by ADP admet-389 138 7 quinine quinine NOUN admet-389 138 8 , , PUNCT admet-389 138 9 quinidine quinidine ADJ admet-389 138 10 and and CCONJ admet-389 138 11 verapamil verapamil NOUN admet-389 138 12 , , PUNCT admet-389 138 13 suggests suggest VERB admet-389 138 14 that that SCONJ admet-389 138 15 these these DET admet-389 138 16 compounds compound NOUN admet-389 138 17 may may AUX admet-389 138 18 share share VERB admet-389 138 19 the the DET admet-389 138 20 same same ADJ admet-389 138 21 substrate substrate NOUN admet-389 138 22 recognition recognition NOUN admet-389 138 23 sites site NOUN admet-389 138 24 . . PUNCT admet-389 139 1 previous previous ADJ admet-389 139 2 reports report NOUN admet-389 139 3 from from ADP admet-389 139 4 our our PRON admet-389 139 5 laboratory laboratory NOUN admet-389 139 6 and and CCONJ admet-389 139 7 others other NOUN admet-389 139 8 have have AUX admet-389 139 9 shown show VERB admet-389 139 10 an an DET admet-389 139 11 interaction interaction NOUN admet-389 139 12 between between ADP admet-389 139 13 quinine quinine NOUN admet-389 139 14 , , PUNCT admet-389 139 15 verapamil verapamil NOUN admet-389 139 16 , , PUNCT admet-389 139 17 quinidine quinidine ADJ admet-389 139 18 and and CCONJ admet-389 139 19 organic organic ADJ admet-389 139 20 cation cation NOUN admet-389 139 21 transporters transporter NOUN admet-389 139 22 [ [ X admet-389 139 23 2 2 NUM admet-389 139 24 , , PUNCT admet-389 139 25 9 9 NUM admet-389 139 26 , , PUNCT admet-389 139 27 10 10 NUM admet-389 139 28 , , PUNCT admet-389 139 29 17 17 NUM admet-389 139 30 ] ] PUNCT admet-389 139 31 . . PUNCT admet-389 140 1 quinine quinine PROPN admet-389 140 2 blocked block VERB admet-389 140 3 et+ et+ NOUN admet-389 140 4 uptake uptake ADJ admet-389 140 5 by by ADP admet-389 140 6 cells cell NOUN admet-389 140 7 overexpressing overexpresse VERB admet-389 140 8 hoct1 hoct1 NOUN admet-389 140 9 or or CCONJ admet-389 140 10 hoct2 hoct2 NOUN admet-389 140 11 with with ADP admet-389 140 12 high high ADJ admet-389 140 13 affinity affinity NOUN admet-389 140 14 [ [ X admet-389 140 15 26 26 NUM admet-389 140 16 ] ] PUNCT admet-389 140 17 . . PUNCT admet-389 141 1 similarly similarly ADV admet-389 141 2 , , PUNCT admet-389 141 3 quinine quinine PROPN admet-389 141 4 and and CCONJ admet-389 141 5 verapamil verapamil NOUN admet-389 141 6 inhibited inhibit VERB admet-389 141 7 hoct1 hoct1 NOUN admet-389 141 8 and and CCONJ admet-389 141 9 hoct2 hoct2 NOUN admet-389 141 10 - - PUNCT admet-389 141 11 mediated mediate VERB admet-389 141 12 uptake uptake NOUN admet-389 141 13 of of ADP admet-389 141 14 rhodamine rhodamine NOUN admet-389 141 15 123 123 NUM admet-389 141 16 with with ADP admet-389 141 17 higher high ADJ admet-389 141 18 inhibitory inhibitory ADJ admet-389 141 19 effects effect NOUN admet-389 141 20 observed observe VERB admet-389 141 21 for for ADP admet-389 141 22 hoct2 hoct2 NOUN admet-389 142 1 [ [ X admet-389 142 2 20 20 NUM admet-389 142 3 ] ] PUNCT admet-389 142 4 . . PUNCT admet-389 143 1 the the DET admet-389 143 2 apparent apparent ADJ admet-389 143 3 discrepancy discrepancy NOUN admet-389 143 4 in in ADP admet-389 143 5 the the DET admet-389 143 6 ic50 ic50 PROPN admet-389 143 7 values value NOUN admet-389 143 8 could could AUX admet-389 143 9 be be AUX admet-389 143 10 attributed attribute VERB admet-389 143 11 to to ADP admet-389 143 12 the the DET admet-389 143 13 fact fact NOUN admet-389 143 14 that that SCONJ admet-389 143 15 amiloride amiloride NOUN admet-389 143 16 , , PUNCT admet-389 143 17 and and CCONJ admet-389 143 18 rhodamines rhodamine VERB admet-389 143 19 bind bind VERB admet-389 143 20 to to ADP admet-389 143 21 different different ADJ admet-389 143 22 , , PUNCT admet-389 143 23 but but CCONJ admet-389 143 24 partially partially ADV admet-389 143 25 overlapping overlap VERB admet-389 143 26 sites site NOUN admet-389 143 27 in in ADP admet-389 143 28 the the DET admet-389 143 29 binding bind VERB admet-389 143 30 pockets pocket NOUN admet-389 143 31 of of ADP admet-389 143 32 the the DET admet-389 143 33 hoct2 hoct2 NOUN admet-389 143 34 . . PUNCT admet-389 144 1 the the DET admet-389 144 2 hoct2 hoct2 NOUN admet-389 144 3 was be AUX admet-389 144 4 previously previously ADV admet-389 144 5 reported report VERB admet-389 144 6 to to PART admet-389 144 7 be be AUX admet-389 144 8 polyspecific polyspecific ADJ admet-389 144 9 , , PUNCT admet-389 144 10 as as SCONJ admet-389 144 11 such such ADJ admet-389 144 12 can can AUX admet-389 144 13 accept accept VERB admet-389 144 14 compounds compound NOUN admet-389 144 15 of of ADP admet-389 144 16 different different ADJ admet-389 144 17 sizes size NOUN admet-389 144 18 and and CCONJ admet-389 144 19 molecular molecular ADJ admet-389 144 20 shapes shape NOUN admet-389 144 21 [ [ X admet-389 144 22 2 2 NUM admet-389 144 23 , , PUNCT admet-389 144 24 4 4 NUM admet-389 144 25 , , PUNCT admet-389 144 26 27 27 NUM admet-389 144 27 ] ] PUNCT admet-389 144 28 . . PUNCT admet-389 145 1 thus thus ADV admet-389 145 2 it it PRON admet-389 145 3 exhibits exhibit VERB admet-389 145 4 large large ADJ admet-389 145 5 variations variation NOUN admet-389 145 6 in in ADP admet-389 145 7 affinity affinity NOUN admet-389 145 8 and and CCONJ admet-389 145 9 turnover turnover NOUN admet-389 145 10 for for ADP admet-389 145 11 different different ADJ admet-389 145 12 compounds compound NOUN admet-389 145 13 . . PUNCT admet-389 146 1 the the DET admet-389 146 2 observed observed ADJ admet-389 146 3 ic50 ic50 PROPN admet-389 146 4 values value NOUN admet-389 146 5 for for ADP admet-389 146 6 quinine quinine NOUN admet-389 146 7 and and CCONJ admet-389 146 8 verapamil verapamil NOUN admet-389 146 9 varied vary VERB admet-389 146 10 with with ADP admet-389 146 11 the the DET admet-389 146 12 reported report VERB admet-389 146 13 ic50 ic50 PROPN admet-389 146 14 of of ADP admet-389 146 15 23 23 NUM admet-389 146 16 µm µm NOUN admet-389 146 17 of of ADP admet-389 146 18 quinine quinine NOUN admet-389 147 1 [ [ X admet-389 147 2 28 28 NUM admet-389 147 3 ] ] PUNCT admet-389 147 4 and and CCONJ admet-389 147 5 85 85 NUM admet-389 147 6 µm µm NOUN admet-389 147 7 of of ADP admet-389 147 8 verapamil verapamil NOUN admet-389 147 9 [ [ X admet-389 147 10 29 29 NUM admet-389 147 11 ] ] PUNCT admet-389 147 12 in in ADP admet-389 147 13 hek hek PROPN admet-389 147 14 293 293 NUM admet-389 147 15 cells cell NOUN admet-389 147 16 expressing express VERB admet-389 147 17 oct oct PROPN admet-389 147 18 2 2 NUM admet-389 147 19 using use VERB admet-389 147 20 n n CCONJ admet-389 147 21 - - PUNCT admet-389 147 22 methylpyridinium methylpyridinium NOUN admet-389 147 23 as as ADP admet-389 147 24 substrate substrate NOUN admet-389 147 25 . . PUNCT admet-389 148 1 similarly similarly ADV admet-389 148 2 the the DET admet-389 148 3 observed observed ADJ admet-389 148 4 ic50 ic50 PROPN admet-389 148 5 of of ADP admet-389 148 6 quinidine quinidine NOUN admet-389 148 7 is be AUX admet-389 148 8 different different ADJ admet-389 148 9 from from ADP admet-389 148 10 13.3 13.3 NUM admet-389 148 11 µm µm NOUN admet-389 148 12 in in ADP admet-389 148 13 a a DET admet-389 148 14 cho cho NOUN admet-389 148 15 cell cell NOUN admet-389 148 16 expressing express VERB admet-389 148 17 oct2 oct2 NOUN admet-389 148 18 using use VERB admet-389 148 19 n n CCONJ admet-389 148 20 - - PUNCT admet-389 148 21 methylpyridinium methylpyridinium NOUN admet-389 148 22 as as ADP admet-389 148 23 substrate substrate NOUN admet-389 148 24 [ [ X admet-389 148 25 30 30 NUM admet-389 148 26 ] ] PUNCT admet-389 148 27 . . PUNCT admet-389 149 1 the the DET admet-389 149 2 presence presence NOUN admet-389 149 3 of of ADP admet-389 149 4 various various ADJ admet-389 149 5 substrate substrate NOUN admet-389 149 6 and and CCONJ admet-389 149 7 inhibitor inhibitor NOUN admet-389 149 8 binding bind VERB admet-389 149 9 sites site NOUN admet-389 149 10 and and CCONJ admet-389 149 11 the the DET admet-389 149 12 complex complex ADJ admet-389 149 13 interactions interaction NOUN admet-389 149 14 between between ADP admet-389 149 15 different different ADJ admet-389 149 16 sites site NOUN admet-389 149 17 explains explain VERB admet-389 149 18 why why SCONJ admet-389 149 19 largely largely ADV admet-389 149 20 different different ADJ admet-389 149 21 ic50 ic50 PROPN admet-389 149 22 values value NOUN admet-389 149 23 are be AUX admet-389 149 24 obtained obtain VERB admet-389 149 25 for for ADP admet-389 149 26 individual individual ADJ admet-389 149 27 transporters transporter NOUN admet-389 149 28 when when SCONJ admet-389 149 29 different different ADJ admet-389 149 30 substrates substrate NOUN admet-389 149 31 are be AUX admet-389 149 32 used use VERB admet-389 149 33 for for ADP admet-389 149 34 transport transport NOUN admet-389 149 35 measurements measurement NOUN admet-389 150 1 [ [ X admet-389 150 2 2,4 2,4 NUM admet-389 150 3 ] ] PUNCT admet-389 150 4 . . PUNCT admet-389 151 1 volk volk PROPN admet-389 151 2 et et PROPN admet-389 151 3 al al PROPN admet-389 151 4 . . PUNCT admet-389 152 1 [ [ X admet-389 152 2 31 31 NUM admet-389 152 3 ] ] PUNCT admet-389 152 4 showed show VERB admet-389 152 5 that that SCONJ admet-389 152 6 the the DET admet-389 152 7 substrate substrate NOUN admet-389 152 8 binding bind VERB admet-389 152 9 site site NOUN admet-389 152 10 of of ADP admet-389 152 11 roct2 roct2 NOUN admet-389 152 12 was be AUX admet-389 152 13 complex complex ADJ admet-389 152 14 , , PUNCT admet-389 152 15 and and CCONJ admet-389 152 16 it it PRON admet-389 152 17 exposes expose VERB admet-389 152 18 different different ADJ admet-389 152 19 interactive interactive ADJ admet-389 152 20 domains domain NOUN admet-389 152 21 for for ADP admet-389 152 22 different different ADJ admet-389 152 23 substrates substrate NOUN admet-389 152 24 and and CCONJ admet-389 152 25 inhibitors inhibitor NOUN admet-389 152 26 . . PUNCT admet-389 153 1 a a DET admet-389 153 2 similar similar ADJ admet-389 153 3 analogy analogy NOUN admet-389 153 4 could could AUX admet-389 153 5 be be AUX admet-389 153 6 true true ADJ admet-389 153 7 for for ADP admet-389 153 8 hoct2 hoct2 NOUN admet-389 153 9 because because SCONJ admet-389 153 10 human human ADJ admet-389 153 11 oct2 oct2 NOUN admet-389 153 12 show show VERB admet-389 153 13 about about ADV admet-389 153 14 90 90 NUM admet-389 153 15 % % NOUN admet-389 153 16 amino amino NOUN admet-389 153 17 acid acid NOUN admet-389 153 18 identities identity NOUN admet-389 153 19 to to ADP admet-389 153 20 the the DET admet-389 153 21 rat rat NOUN admet-389 153 22 oct2 oct2 NOUN admet-389 154 1 [ [ X admet-389 154 2 32 32 NUM admet-389 154 3 ] ] PUNCT admet-389 154 4 . . PUNCT admet-389 155 1 several several ADJ admet-389 155 2 of of ADP admet-389 155 3 these these DET admet-389 155 4 amino amino ADJ admet-389 155 5 acids acid NOUN admet-389 155 6 in in ADP admet-389 155 7 the the DET admet-389 155 8 transporter transporter NOUN admet-389 155 9 transmembrane transmembrane NOUN admet-389 155 10 helix helix NOUN admet-389 155 11 contain contain VERB admet-389 155 12 highand highand ADJ admet-389 155 13 lowaffinity lowaffinity NOUN admet-389 155 14 substrate substrate NOUN admet-389 155 15 and/or and/or CCONJ admet-389 155 16 inhibitor inhibitor NOUN admet-389 155 17 binding bind VERB admet-389 155 18 sites site NOUN admet-389 155 19 that that PRON admet-389 155 20 are be AUX admet-389 155 21 involved involve VERB admet-389 155 22 in in ADP admet-389 155 23 substrate substrate NOUN admet-389 155 24 and/or and/or CCONJ admet-389 155 25 inhibitor inhibitor NOUN admet-389 155 26 binding binding NOUN admet-389 155 27 of of ADP admet-389 155 28 the the DET admet-389 155 29 oct oct PROPN admet-389 155 30 transporters transporter NOUN admet-389 155 31 [ [ X admet-389 155 32 4,22 4,22 X admet-389 155 33 ] ] X admet-389 155 34 . . PUNCT admet-389 156 1 in in ADP admet-389 156 2 line line NOUN admet-389 156 3 with with ADP admet-389 156 4 the the DET admet-389 156 5 conformational conformational ADJ admet-389 156 6 analogy analogy NOUN admet-389 156 7 , , PUNCT admet-389 156 8 thévenod thévenod PROPN admet-389 156 9 and and CCONJ admet-389 156 10 co co NOUN admet-389 156 11 - - NOUN admet-389 156 12 workers worker NOUN admet-389 156 13 maintained maintain VERB admet-389 156 14 that that PRON admet-389 156 15 octs octs AUX admet-389 156 16 acquire acquire VERB admet-389 156 17 minimum minimum NOUN admet-389 156 18 of of ADP admet-389 156 19 three three NUM admet-389 156 20 conformational conformational ADJ admet-389 156 21 states state NOUN admet-389 156 22 : : PUNCT admet-389 156 23 one one NUM admet-389 156 24 state state NOUN admet-389 156 25 with with ADP admet-389 156 26 an an DET admet-389 156 27 outward outward ADV admet-389 156 28 - - PUNCT admet-389 156 29 open open ADJ admet-389 156 30 binding bind VERB admet-389 156 31 cleft cleft NOUN admet-389 156 32 containing contain VERB admet-389 156 33 an an DET admet-389 156 34 innermost innermost ADJ admet-389 156 35 binding bind VERB admet-389 156 36 pocket pocket NOUN admet-389 156 37 where where SCONJ admet-389 156 38 transported transport VERB admet-389 156 39 cations cation NOUN admet-389 156 40 bind bind VERB admet-389 156 41 and and CCONJ admet-389 156 42 additional additional ADJ admet-389 156 43 more more ADV admet-389 156 44 peripherally peripherally ADV admet-389 156 45 localized localized ADJ admet-389 156 46 binding bind VERB admet-389 156 47 sites site NOUN admet-389 156 48 for for ADP admet-389 156 49 organic organic ADJ admet-389 156 50 cations cation NOUN admet-389 156 51 which which PRON admet-389 156 52 may may AUX admet-389 156 53 overlap overlap VERB admet-389 156 54 with with ADP admet-389 156 55 domains domain NOUN admet-389 156 56 in in ADP admet-389 156 57 the the DET admet-389 156 58 innermost innermost ADJ admet-389 156 59 binding bind VERB admet-389 156 60 pocket pocket NOUN admet-389 156 61 [ [ X admet-389 156 62 31 31 NUM admet-389 156 63 ] ] PUNCT admet-389 156 64 . . PUNCT admet-389 157 1 they they PRON admet-389 157 2 reported report VERB admet-389 157 3 that that SCONJ admet-389 157 4 structurally structurally ADV admet-389 157 5 different different ADJ admet-389 157 6 transported transport VERB admet-389 157 7 cations cation NOUN admet-389 157 8 induce induce VERB admet-389 157 9 substrates substrate NOUN admet-389 157 10 due due ADP admet-389 157 11 to to ADP admet-389 157 12 the the DET admet-389 157 13 interaction interaction NOUN admet-389 157 14 of of ADP admet-389 157 15 different different ADJ admet-389 157 16 substrates substrate NOUN admet-389 157 17 with with ADP admet-389 157 18 partially partially ADV admet-389 157 19 different different ADJ admet-389 157 20 amino amino NOUN admet-389 157 21 acids acid NOUN admet-389 158 1 [ [ X admet-389 158 2 31 31 NUM admet-389 158 3 ] ] PUNCT admet-389 158 4 . . PUNCT admet-389 159 1 in in ADP admet-389 159 2 addition addition NOUN admet-389 159 3 to to ADP admet-389 159 4 overlapping overlap VERB admet-389 159 5 sites site NOUN admet-389 159 6 , , PUNCT admet-389 159 7 they they PRON admet-389 159 8 also also ADV admet-389 159 9 reported report VERB admet-389 159 10 that that SCONJ admet-389 159 11 the the DET admet-389 159 12 inhibitor inhibitor NOUN admet-389 159 13 potencies potency NOUN admet-389 159 14 of of ADP admet-389 159 15 oct oct PROPN admet-389 159 16 ligands ligand NOUN admet-389 159 17 are be AUX admet-389 159 18 highly highly ADV admet-389 159 19 dependent dependent ADJ admet-389 159 20 on on ADP admet-389 159 21 the the DET admet-389 159 22 substrate substrate NOUN admet-389 159 23 employed employ VERB admet-389 159 24 for for ADP admet-389 159 25 uptake uptake ADJ admet-389 159 26 studies study NOUN admet-389 159 27 . . PUNCT admet-389 160 1 the the DET admet-389 160 2 uptake uptake NOUN admet-389 160 3 of of ADP admet-389 160 4 the the DET admet-389 160 5 fluorescent fluorescent ADJ admet-389 160 6 compounds compound NOUN admet-389 160 7 ( ( PUNCT admet-389 160 8 amiloride amiloride ADJ admet-389 160 9 , , PUNCT admet-389 160 10 rhodamine rhodamine NOUN admet-389 160 11 6 6 NUM admet-389 160 12 g g NOUN admet-389 160 13 and and CCONJ admet-389 160 14 rhodamine rhodamine NOUN admet-389 160 15 123 123 NUM admet-389 160 16 ) ) PUNCT admet-389 160 17 was be AUX admet-389 160 18 oct2mediated oct2mediate VERB admet-389 160 19 . . PUNCT admet-389 161 1 this this PRON admet-389 161 2 was be AUX admet-389 161 3 evident evident ADJ admet-389 161 4 in in ADP admet-389 161 5 the the DET admet-389 161 6 concentrationdependent concentrationdependent NOUN admet-389 161 7 uptake uptake NOUN admet-389 161 8 of of ADP admet-389 161 9 the the DET admet-389 161 10 compounds compound NOUN admet-389 161 11 , , PUNCT admet-389 161 12 as as ADV admet-389 161 13 well well ADV admet-389 161 14 as as ADP admet-389 161 15 their their PRON admet-389 161 16 uptake uptake ADJ admet-389 161 17 inhibition inhibition NOUN admet-389 161 18 by by ADP admet-389 161 19 classical classical ADJ admet-389 161 20 organic organic ADJ admet-389 161 21 cationic cationic ADJ admet-389 161 22 inhibitors inhibitor NOUN admet-389 161 23 . . PUNCT admet-389 162 1 remigius remigius PROPN admet-389 162 2 agu agu PROPN admet-389 162 3 et et PROPN admet-389 162 4 al al PROPN admet-389 162 5 . . PROPN admet-389 162 6 admet admet PROPN admet-389 162 7 & & CCONJ admet-389 162 8 dmpk dmpk PROPN admet-389 162 9 5(2 5(2 NUM admet-389 162 10 ) ) PUNCT admet-389 162 11 ( ( PUNCT admet-389 162 12 2017 2017 NUM admet-389 162 13 ) ) PUNCT admet-389 162 14 135 135 NUM admet-389 162 15 - - SYM admet-389 162 16 145 145 NUM admet-389 162 17 142 142 NUM admet-389 162 18 figure figure NOUN admet-389 162 19 4 4 NUM admet-389 162 20 . . PUNCT admet-389 162 21 inhibition inhibition NOUN admet-389 162 22 of of ADP admet-389 162 23 the the DET admet-389 162 24 fluorescent fluorescent NOUN admet-389 162 25 compounds compound VERB admet-389 162 26 uptake uptake ADJ admet-389 162 27 in in ADP admet-389 162 28 cho cho NOUN admet-389 162 29 cells cell NOUN admet-389 162 30 stably stably ADV admet-389 162 31 expressing express VERB admet-389 162 32 hoct2 hoct2 NOUN admet-389 162 33 by by ADP admet-389 162 34 oct oct PROPN admet-389 162 35 / / SYM admet-389 162 36 octn octn NOUN admet-389 162 37 inhibitors inhibitor NOUN admet-389 162 38 . . PUNCT admet-389 163 1 cells cell NOUN admet-389 163 2 were be AUX admet-389 163 3 pre pre ADJ admet-389 163 4 - - VERB admet-389 163 5 incubated incubate VERB admet-389 163 6 with with ADP admet-389 163 7 inhibitors inhibitor NOUN admet-389 163 8 at at ADP admet-389 163 9 1 1 NUM admet-389 163 10 mm mm NOUN admet-389 163 11 for for ADP admet-389 163 12 25 25 NUM admet-389 163 13 min min NOUN admet-389 163 14 followed follow VERB admet-389 163 15 by by ADP admet-389 163 16 an an DET admet-389 163 17 additional additional ADJ admet-389 163 18 7 7 NUM admet-389 163 19 min min NOUN admet-389 163 20 incubation incubation NOUN admet-389 163 21 with with ADP admet-389 163 22 the the DET admet-389 163 23 respective respective ADJ admet-389 163 24 fluorescent fluorescent NOUN admet-389 163 25 compounds compound NOUN admet-389 163 26 . . PUNCT admet-389 164 1 subsequently subsequently ADV admet-389 164 2 , , PUNCT admet-389 164 3 the the DET admet-389 164 4 cells cell NOUN admet-389 164 5 were be AUX admet-389 164 6 washed wash VERB admet-389 164 7 with with ADP admet-389 164 8 ice ice NOUN admet-389 164 9 - - PUNCT admet-389 164 10 cold cold ADJ admet-389 164 11 uptake uptake ADJ admet-389 164 12 buffer buffer NOUN admet-389 164 13 followed follow VERB admet-389 164 14 by by ADP admet-389 164 15 extraction extraction NOUN admet-389 164 16 with with ADP admet-389 164 17 triton triton PROPN admet-389 164 18 x-100/0.1 x-100/0.1 PROPN admet-389 164 19 m m PROPN admet-389 164 20 naoh naoh PROPN admet-389 164 21 . . PUNCT admet-389 165 1 data datum NOUN admet-389 165 2 represent represent VERB admet-389 165 3 mean mean PROPN admet-389 165 4 ± ± NUM admet-389 165 5 sd sd NOUN admet-389 165 6 ; ; PUNCT admet-389 165 7 n n NOUN admet-389 165 8 = = SYM admet-389 165 9 3 3 X admet-389 165 10 . . X admet-389 165 11 figure figure NOUN admet-389 165 12 5 5 NUM admet-389 165 13 . . PUNCT admet-389 165 14 inhibition inhibition NOUN admet-389 165 15 of of ADP admet-389 165 16 the the DET admet-389 165 17 fluorescent fluorescent NOUN admet-389 165 18 compounds compound VERB admet-389 165 19 uptake uptake ADJ admet-389 165 20 in in ADP admet-389 165 21 cho cho NOUN admet-389 165 22 cells cell NOUN admet-389 165 23 stably stably ADV admet-389 165 24 expressing express VERB admet-389 165 25 hoct2 hoct2 NOUN admet-389 165 26 by by ADP admet-389 165 27 quinidine quinidine NOUN admet-389 165 28 . . PUNCT admet-389 166 1 cells cell NOUN admet-389 166 2 were be AUX admet-389 166 3 pre pre ADJ admet-389 166 4 - - VERB admet-389 166 5 incubated incubate VERB admet-389 166 6 with with ADP admet-389 166 7 inhibitor inhibitor NOUN admet-389 166 8 ( ( PUNCT admet-389 166 9 quinidine quinidine NOUN admet-389 166 10 ) ) PUNCT admet-389 166 11 for for ADP admet-389 166 12 25 25 NUM admet-389 166 13 min min NOUN admet-389 166 14 followed follow VERB admet-389 166 15 by by ADP admet-389 166 16 additional additional ADJ admet-389 166 17 7 7 NUM admet-389 166 18 min min NOUN admet-389 166 19 incubation incubation NOUN admet-389 166 20 with with ADP admet-389 166 21 the the DET admet-389 166 22 respective respective ADJ admet-389 166 23 dyes dye NOUN admet-389 166 24 . . PUNCT admet-389 167 1 subsequently subsequently ADV admet-389 167 2 , , PUNCT admet-389 167 3 the the DET admet-389 167 4 cells cell NOUN admet-389 167 5 were be AUX admet-389 167 6 washed wash VERB admet-389 167 7 with with ADP admet-389 167 8 ice ice NOUN admet-389 167 9 - - PUNCT admet-389 167 10 cold cold ADJ admet-389 167 11 uptake uptake ADJ admet-389 167 12 buffer buffer NOUN admet-389 167 13 followed follow VERB admet-389 167 14 by by ADP admet-389 167 15 extraction extraction NOUN admet-389 167 16 with with ADP admet-389 167 17 triton triton PROPN admet-389 167 18 x-100/0.1 x-100/0.1 PROPN admet-389 167 19 m m PROPN admet-389 167 20 naoh naoh PROPN admet-389 167 21 . . PUNCT admet-389 168 1 data datum NOUN admet-389 168 2 represent represent VERB admet-389 168 3 mean mean PROPN admet-389 168 4 ± ± NUM admet-389 168 5 sd sd NOUN admet-389 168 6 ; ; PUNCT admet-389 168 7 n n NOUN admet-389 168 8 = = SYM admet-389 168 9 3 3 X admet-389 168 10 . . X admet-389 168 11 admet admet PROPN admet-389 168 12 & & CCONJ admet-389 168 13 dmpk dmpk PROPN admet-389 168 14 5(2 5(2 NUM admet-389 168 15 ) ) PUNCT admet-389 168 16 ( ( PUNCT admet-389 168 17 2017 2017 NUM admet-389 168 18 ) ) PUNCT admet-389 168 19 135 135 NUM admet-389 168 20 - - SYM admet-389 168 21 145 145 NUM admet-389 168 22 fluorescent fluorescent ADJ admet-389 168 23 organic organic ADJ admet-389 168 24 cations cation NOUN admet-389 168 25 for for ADP admet-389 168 26 human human ADJ admet-389 168 27 oct2 oct2 NOUN admet-389 168 28 transporters transporter NOUN admet-389 168 29 screening screen VERB admet-389 168 30 doi doi NOUN admet-389 168 31 : : PUNCT admet-389 168 32 10.5599 10.5599 NUM admet-389 168 33 / / SYM admet-389 168 34 admet.5.2.389 admet.5.2.389 NOUN admet-389 168 35 143 143 NUM admet-389 168 36 figure figure NOUN admet-389 168 37 6 6 NUM admet-389 168 38 . . PUNCT admet-389 168 39 inhibition inhibition NOUN admet-389 168 40 of of ADP admet-389 168 41 the the DET admet-389 168 42 fluorescent fluorescent NOUN admet-389 168 43 compounds compound VERB admet-389 168 44 uptake uptake ADJ admet-389 168 45 in in ADP admet-389 168 46 cho cho NOUN admet-389 168 47 cells cell NOUN admet-389 168 48 stably stably ADV admet-389 168 49 expressing express VERB admet-389 168 50 hoct2 hoct2 NOUN admet-389 168 51 by by ADP admet-389 168 52 quinine quinine NOUN admet-389 168 53 . . PUNCT admet-389 169 1 cells cell NOUN admet-389 169 2 were be AUX admet-389 169 3 pre pre ADJ admet-389 169 4 - - VERB admet-389 169 5 incubated incubate VERB admet-389 169 6 with with ADP admet-389 169 7 inhibitor inhibitor NOUN admet-389 169 8 ( ( PUNCT admet-389 169 9 quinine quinine PROPN admet-389 169 10 ) ) PUNCT admet-389 169 11 for for ADP admet-389 169 12 25 25 NUM admet-389 169 13 min min NOUN admet-389 169 14 followed follow VERB admet-389 169 15 by by ADP admet-389 169 16 an an DET admet-389 169 17 additional additional ADJ admet-389 169 18 7 7 NUM admet-389 169 19 min min NOUN admet-389 169 20 incubation incubation NOUN admet-389 169 21 with with ADP admet-389 169 22 the the DET admet-389 169 23 respective respective ADJ admet-389 169 24 dyes dye NOUN admet-389 169 25 . . PUNCT admet-389 170 1 subsequently subsequently ADV admet-389 170 2 , , PUNCT admet-389 170 3 the the DET admet-389 170 4 cells cell NOUN admet-389 170 5 were be AUX admet-389 170 6 washed wash VERB admet-389 170 7 with with ADP admet-389 170 8 ice ice NOUN admet-389 170 9 - - PUNCT admet-389 170 10 cold cold ADJ admet-389 170 11 uptake uptake ADJ admet-389 170 12 buffer buffer NOUN admet-389 170 13 followed follow VERB admet-389 170 14 by by ADP admet-389 170 15 extraction extraction NOUN admet-389 170 16 with with ADP admet-389 170 17 triton triton PROPN admet-389 170 18 x-100/0.1 x-100/0.1 PROPN admet-389 170 19 m m PROPN admet-389 170 20 naoh naoh PROPN admet-389 170 21 . . PUNCT admet-389 171 1 data datum NOUN admet-389 171 2 represent represent VERB admet-389 171 3 mean mean PROPN admet-389 171 4 ± ± NUM admet-389 171 5 sd sd NOUN admet-389 171 6 ; ; PUNCT admet-389 171 7 n n NOUN admet-389 171 8 = = SYM admet-389 171 9 3 3 X admet-389 171 10 . . X admet-389 171 11 figure figure NOUN admet-389 171 12 7 7 NUM admet-389 171 13 . . PUNCT admet-389 171 14 inhibition inhibition NOUN admet-389 171 15 of of ADP admet-389 171 16 the the DET admet-389 171 17 fluorescent fluorescent NOUN admet-389 171 18 compounds compound VERB admet-389 171 19 uptake uptake ADJ admet-389 171 20 in in ADP admet-389 171 21 cho cho NOUN admet-389 171 22 cells cell NOUN admet-389 171 23 stably stably ADV admet-389 171 24 expressing express VERB admet-389 171 25 hoct2 hoct2 NOUN admet-389 171 26 by by ADP admet-389 171 27 verapamil verapamil NOUN admet-389 171 28 . . PUNCT admet-389 172 1 cells cell NOUN admet-389 172 2 were be AUX admet-389 172 3 pre pre ADJ admet-389 172 4 - - VERB admet-389 172 5 incubated incubate VERB admet-389 172 6 with with ADP admet-389 172 7 inhibitor inhibitor NOUN admet-389 172 8 ( ( PUNCT admet-389 172 9 verapamil verapamil NOUN admet-389 172 10 ) ) PUNCT admet-389 172 11 for for ADP admet-389 172 12 25 25 NUM admet-389 172 13 min min NOUN admet-389 172 14 followed follow VERB admet-389 172 15 by by ADP admet-389 172 16 additional additional ADJ admet-389 172 17 7 7 NUM admet-389 172 18 min min NOUN admet-389 172 19 incubation incubation NOUN admet-389 172 20 with with ADP admet-389 172 21 the the DET admet-389 172 22 respective respective ADJ admet-389 172 23 dyes dye NOUN admet-389 172 24 . . PUNCT admet-389 173 1 subsequently subsequently ADV admet-389 173 2 , , PUNCT admet-389 173 3 the the DET admet-389 173 4 cells cell NOUN admet-389 173 5 were be AUX admet-389 173 6 washed wash VERB admet-389 173 7 with with ADP admet-389 173 8 ice ice NOUN admet-389 173 9 - - PUNCT admet-389 173 10 cold cold ADJ admet-389 173 11 uptake uptake ADJ admet-389 173 12 buffer buffer NOUN admet-389 173 13 followed follow VERB admet-389 173 14 by by ADP admet-389 173 15 extraction extraction NOUN admet-389 173 16 with with ADP admet-389 173 17 triton triton PROPN admet-389 173 18 x-100/0.1 x-100/0.1 PROPN admet-389 173 19 m m PROPN admet-389 173 20 naoh naoh PROPN admet-389 173 21 . . PUNCT admet-389 174 1 data datum NOUN admet-389 174 2 represent represent VERB admet-389 174 3 mean mean PROPN admet-389 174 4 ± ± NUM admet-389 174 5 sd sd NOUN admet-389 174 6 ; ; PUNCT admet-389 174 7 n n NOUN admet-389 174 8 = = SYM admet-389 174 9 3 3 X admet-389 174 10 . . X admet-389 174 11 remigius remigius PROPN admet-389 174 12 agu agu PROPN admet-389 174 13 et et PROPN admet-389 174 14 al al PROPN admet-389 174 15 . . PROPN admet-389 174 16 admet admet PROPN admet-389 174 17 & & CCONJ admet-389 174 18 dmpk dmpk PROPN admet-389 174 19 5(2 5(2 NUM admet-389 174 20 ) ) PUNCT admet-389 174 21 ( ( PUNCT admet-389 174 22 2017 2017 NUM admet-389 174 23 ) ) PUNCT admet-389 174 24 135 135 NUM admet-389 174 25 - - SYM admet-389 174 26 145 145 NUM admet-389 174 27 144 144 NUM admet-389 174 28 conclusions conclusion NOUN admet-389 174 29 in in ADP admet-389 174 30 this this DET admet-389 174 31 study study NOUN admet-389 174 32 we we PRON admet-389 174 33 investigated investigate VERB admet-389 174 34 the the DET admet-389 174 35 potential potential ADJ admet-389 174 36 use use NOUN admet-389 174 37 of of ADP admet-389 174 38 amiloride amiloride NOUN admet-389 174 39 , , PUNCT admet-389 174 40 rhodamine rhodamine NOUN admet-389 174 41 6 6 NUM admet-389 174 42 g g NOUN admet-389 174 43 and and CCONJ admet-389 174 44 rhodamine rhodamine NOUN admet-389 174 45 123 123 NUM admet-389 174 46 as as ADP admet-389 174 47 nonradioactive nonradioactive ADJ admet-389 174 48 probes probe NOUN admet-389 174 49 for for ADP admet-389 174 50 characterizing characterize VERB admet-389 174 51 human human ADJ admet-389 174 52 organic organic ADJ admet-389 174 53 cation cation NOUN admet-389 174 54 transporter transporter NOUN admet-389 174 55 isoform isoform NOUN admet-389 174 56 , , PUNCT admet-389 174 57 hoct2 hoct2 NOUN admet-389 174 58 using use VERB admet-389 174 59 cho cho NOUN admet-389 174 60 cells cell NOUN admet-389 174 61 stably stably ADV admet-389 174 62 - - PUNCT admet-389 174 63 expressing express VERB admet-389 174 64 the the DET admet-389 174 65 transporter transporter NOUN admet-389 174 66 . . PUNCT admet-389 175 1 our our PRON admet-389 175 2 data datum NOUN admet-389 175 3 suggest suggest VERB admet-389 175 4 that that SCONJ admet-389 175 5 the the DET admet-389 175 6 investigated investigate VERB admet-389 175 7 fluorescent fluorescent NOUN admet-389 175 8 organic organic ADJ admet-389 175 9 cations cation NOUN admet-389 175 10 were be AUX admet-389 175 11 efficiently efficiently ADV admet-389 175 12 taken take VERB admet-389 175 13 up up ADP admet-389 175 14 by by ADP admet-389 175 15 the the DET admet-389 175 16 cho cho NOUN admet-389 175 17 cells cell NOUN admet-389 175 18 . . PUNCT admet-389 176 1 however however ADV admet-389 176 2 , , PUNCT admet-389 176 3 based base VERB admet-389 176 4 on on ADP admet-389 176 5 their their PRON admet-389 176 6 fluorescence fluorescence NOUN admet-389 176 7 , , PUNCT admet-389 176 8 affinities affinity NOUN admet-389 176 9 ( ( PUNCT admet-389 176 10 km km NOUN admet-389 176 11 / / SYM admet-389 176 12 ic50s ic50 NOUN admet-389 176 13 ) ) PUNCT admet-389 176 14 , , PUNCT admet-389 176 15 aqueous aqueous ADJ admet-389 176 16 solubility solubility NOUN admet-389 176 17 , , PUNCT admet-389 176 18 ease ease NOUN admet-389 176 19 of of ADP admet-389 176 20 use use NOUN admet-389 176 21 at at ADP admet-389 176 22 high high ADJ admet-389 176 23 concentrations concentration NOUN admet-389 176 24 and and CCONJ admet-389 176 25 recognition recognition NOUN admet-389 176 26 as as ADP admet-389 176 27 hoct2 hoct2 NOUN admet-389 176 28 substrates substrate NOUN admet-389 176 29 by by ADP admet-389 176 30 fda fda PROPN admet-389 176 31 and and CCONJ admet-389 176 32 european european PROPN admet-389 176 33 union union PROPN admet-389 176 34 and and CCONJ admet-389 176 35 the the DET admet-389 176 36 possibility possibility NOUN admet-389 176 37 to to PART admet-389 176 38 be be AUX admet-389 176 39 used use VERB admet-389 176 40 for for ADP admet-389 176 41 in in ADP admet-389 176 42 vivo vivo ADJ admet-389 176 43 human human ADJ admet-389 176 44 studies study NOUN admet-389 176 45 , , PUNCT admet-389 176 46 we we PRON admet-389 176 47 recommend recommend VERB admet-389 176 48 the the DET admet-389 176 49 use use NOUN admet-389 176 50 of of ADP admet-389 176 51 amiloride amiloride NOUN admet-389 176 52 as as ADP admet-389 176 53 an an DET admet-389 176 54 acceptable acceptable ADJ admet-389 176 55 non non ADJ admet-389 176 56 - - ADJ admet-389 176 57 radioactive radioactive ADJ admet-389 176 58 substrate substrate NOUN admet-389 176 59 for for ADP admet-389 176 60 screening screen VERB admet-389 176 61 hoct2 hoct2 NOUN admet-389 176 62 expression expression NOUN admet-389 176 63 in in ADP admet-389 176 64 cells cell NOUN admet-389 176 65 using use VERB admet-389 176 66 fluorimeters fluorimeter NOUN admet-389 176 67 . . PUNCT admet-389 177 1 although although SCONJ admet-389 177 2 amiloride amiloride NOUN admet-389 177 3 is be AUX admet-389 177 4 a a DET admet-389 177 5 substrate substrate NOUN admet-389 177 6 for for ADP admet-389 177 7 organic organic ADJ admet-389 177 8 cation cation NOUN admet-389 177 9 transporters transporter NOUN admet-389 177 10 , , PUNCT admet-389 177 11 it it PRON admet-389 177 12 also also ADV admet-389 177 13 inhibits inhibit VERB admet-389 177 14 sodium sodium NOUN admet-389 177 15 reabsorption reabsorption NOUN admet-389 177 16 in in ADP admet-389 177 17 the the DET admet-389 177 18 kidney kidney NOUN admet-389 177 19 via via ADP admet-389 177 20 the the DET admet-389 177 21 blockade blockade NOUN admet-389 177 22 of of ADP admet-389 177 23 na na INTJ admet-389 177 24 + + CCONJ admet-389 177 25 /h /h SYM admet-389 177 26 + + CCONJ admet-389 177 27 antiporter antiporter NOUN admet-389 177 28 located locate VERB admet-389 177 29 on on ADP admet-389 177 30 the the DET admet-389 177 31 apical apical ADJ admet-389 177 32 side side NOUN admet-389 177 33 of of ADP admet-389 177 34 the the DET admet-389 177 35 proximal proximal ADJ admet-389 177 36 tubules tubule NOUN admet-389 177 37 . . PUNCT admet-389 178 1 from from ADP admet-389 178 2 drug drug NOUN admet-389 178 3 interaction interaction NOUN admet-389 178 4 perspective perspective NOUN admet-389 178 5 , , PUNCT admet-389 178 6 care care NOUN admet-389 178 7 should should AUX admet-389 178 8 be be AUX admet-389 178 9 taken take VERB admet-389 178 10 in in ADP admet-389 178 11 geriatrics geriatric NOUN admet-389 178 12 or or CCONJ admet-389 178 13 patients patient NOUN admet-389 178 14 with with ADP admet-389 178 15 hyperkalaemia hyperkalaemia NOUN admet-389 178 16 or or CCONJ admet-389 178 17 those those PRON admet-389 178 18 receiving receive VERB admet-389 178 19 other other ADJ admet-389 178 20 potassium potassium NOUN admet-389 178 21 - - PUNCT admet-389 178 22 sparing spare VERB admet-389 178 23 agents agent NOUN admet-389 178 24 when when SCONJ admet-389 178 25 using use VERB admet-389 178 26 this this DET admet-389 178 27 drug drug NOUN admet-389 178 28 . . PUNCT admet-389 179 1 furthermore furthermore ADV admet-389 179 2 , , PUNCT admet-389 179 3 considering consider VERB admet-389 179 4 the the DET admet-389 179 5 mechanism mechanism NOUN admet-389 179 6 of of ADP admet-389 179 7 action action NOUN admet-389 179 8 of of ADP admet-389 179 9 amiloride amiloride NOUN admet-389 179 10 and and CCONJ admet-389 179 11 the the DET admet-389 179 12 role role NOUN admet-389 179 13 and and CCONJ admet-389 179 14 wide wide ADJ admet-389 179 15 expression expression NOUN admet-389 179 16 of of ADP admet-389 179 17 oct2 oct2 NOUN admet-389 179 18 in in ADP admet-389 179 19 kidney kidney NOUN admet-389 179 20 , , PUNCT admet-389 179 21 the the DET admet-389 179 22 use use NOUN admet-389 179 23 of of ADP admet-389 179 24 this this DET admet-389 179 25 drug drug NOUN admet-389 179 26 along along ADP admet-389 179 27 with with ADP admet-389 179 28 clinically clinically ADV admet-389 179 29 used use VERB admet-389 179 30 potent potent ADJ admet-389 179 31 oct2 oct2 NOUN admet-389 179 32 inhibitors inhibitor NOUN admet-389 179 33 ( ( PUNCT admet-389 179 34 e.g. e.g. ADV admet-389 179 35 metformin metformin NOUN admet-389 179 36 ) ) PUNCT admet-389 179 37 , , PUNCT admet-389 179 38 should should AUX admet-389 179 39 be be AUX admet-389 179 40 used use VERB admet-389 179 41 with with ADP admet-389 179 42 caution caution NOUN admet-389 179 43 to to PART admet-389 179 44 avoid avoid VERB admet-389 179 45 clinically clinically ADV admet-389 179 46 significant significant ADJ admet-389 179 47 interactions interaction NOUN admet-389 179 48 – – PUNCT admet-389 179 49 in in ADP admet-389 179 50 this this DET admet-389 179 51 case case NOUN admet-389 179 52 hypoglycaemic hypoglycaemic ADJ admet-389 179 53 ( ( PUNCT admet-389 179 54 reduced reduce VERB admet-389 179 55 metformin metformin NOUN admet-389 179 56 clearance clearance NOUN admet-389 179 57 ) ) PUNCT admet-389 179 58 or or CCONJ admet-389 179 59 elevated elevate VERB admet-389 179 60 amiloride amiloride ADJ admet-389 179 61 concentration concentration NOUN admet-389 179 62 and and CCONJ admet-389 179 63 duration duration NOUN admet-389 179 64 ( ( PUNCT admet-389 179 65 due due ADP admet-389 179 66 to to ADP admet-389 179 67 metformin metformin NOUN admet-389 179 68 inhibition inhibition NOUN admet-389 179 69 ) ) PUNCT admet-389 179 70 , , PUNCT admet-389 179 71 which which PRON admet-389 179 72 may may AUX admet-389 179 73 be be AUX admet-389 179 74 an an DET admet-389 179 75 advantage advantage NOUN admet-389 179 76 considering consider VERB admet-389 179 77 amiloride amiloride NOUN admet-389 179 78 ’s ’s PART admet-389 179 79 short short ADJ admet-389 179 80 duration duration NOUN admet-389 179 81 of of ADP admet-389 179 82 action action NOUN admet-389 179 83 . . PUNCT admet-389 180 1 it it PRON admet-389 180 2 is be AUX admet-389 180 3 also also ADV admet-389 180 4 important important ADJ admet-389 180 5 to to PART admet-389 180 6 note note VERB admet-389 180 7 that that SCONJ admet-389 180 8 oct2 oct2 NOUN admet-389 180 9 transporters transporter NOUN admet-389 180 10 are be AUX admet-389 180 11 mainly mainly ADV admet-389 180 12 expressed express VERB admet-389 180 13 basolaterally basolaterally ADV admet-389 180 14 in in ADP admet-389 180 15 the the DET admet-389 180 16 kidney kidney NOUN admet-389 180 17 tissues tissue NOUN admet-389 180 18 , , PUNCT admet-389 180 19 which which PRON admet-389 180 20 makes make VERB admet-389 180 21 individuals individual NOUN admet-389 180 22 with with ADP admet-389 180 23 polymorphisms polymorphism NOUN admet-389 180 24 of of ADP admet-389 180 25 this this DET admet-389 180 26 transporter transporter NOUN admet-389 180 27 prone prone ADJ admet-389 180 28 to to ADP admet-389 180 29 clinically clinically ADV admet-389 180 30 significant significant ADJ admet-389 180 31 drug drug NOUN admet-389 180 32 toxicity toxicity NOUN admet-389 180 33 due due ADP admet-389 180 34 to to ADP admet-389 180 35 reduced reduce VERB admet-389 180 36 renal renal ADJ admet-389 180 37 clearance clearance NOUN admet-389 180 38 . . PUNCT admet-389 181 1 overall overall ADV admet-389 181 2 , , PUNCT admet-389 181 3 observed observe VERB admet-389 181 4 clinical clinical ADJ admet-389 181 5 manifestation manifestation NOUN admet-389 181 6 of of ADP admet-389 181 7 genetic genetic ADJ admet-389 181 8 polymorphisms polymorphism NOUN admet-389 181 9 involving involve VERB admet-389 181 10 amiloride amiloride NOUN admet-389 181 11 may may AUX admet-389 181 12 depend depend VERB admet-389 181 13 on on ADP admet-389 181 14 whether whether SCONJ admet-389 181 15 the the DET admet-389 181 16 genes gene NOUN admet-389 181 17 involved involve VERB admet-389 181 18 affects affect VERB admet-389 181 19 na+/h+ na+/h+ ADV admet-389 181 20 antiporter antiporter NOUN admet-389 181 21 located locate VERB admet-389 181 22 apically apically ADV admet-389 181 23 or or CCONJ admet-389 181 24 oct2 oct2 NOUN admet-389 181 25 located locate VERB admet-389 181 26 basolaterally basolaterally ADV admet-389 181 27 . . PUNCT admet-389 182 1 acknowledgements acknowledgement NOUN admet-389 182 2 : : PUNCT admet-389 182 3 this this DET admet-389 182 4 project project NOUN admet-389 182 5 was be AUX admet-389 182 6 funded fund VERB admet-389 182 7 with with ADP admet-389 182 8 a a DET admet-389 182 9 grant grant NOUN admet-389 182 10 from from ADP admet-389 182 11 dalhousie dalhousie PROPN admet-389 182 12 university university PROPN admet-389 182 13 pharmacy pharmacy NOUN admet-389 182 14 endowment endowment NOUN admet-389 182 15 fund fund PROPN admet-389 182 16 . . PUNCT admet-389 183 1 references reference NOUN admet-389 183 2 [ [ X admet-389 183 3 1 1 NUM admet-389 183 4 ] ] X admet-389 183 5 g. g. PROPN admet-389 183 6 ciarimboli ciarimboli PROPN admet-389 183 7 . . PUNCT admet-389 184 1 xenobiotica xenobiotica PROPN admet-389 184 2 , , PUNCT admet-389 184 3 38 38 NUM admet-389 184 4 ( ( PUNCT admet-389 184 5 2008 2008 NUM admet-389 184 6 ) ) PUNCT admet-389 184 7 936–971 936–971 NUM admet-389 184 8 . . PUNCT admet-389 185 1 [ [ X admet-389 185 2 2 2 X admet-389 185 3 ] ] PUNCT admet-389 185 4 j. j. PROPN admet-389 185 5 biermann biermann PROPN admet-389 185 6 , , PUNCT admet-389 185 7 d. d. PROPN admet-389 185 8 lang lang PROPN admet-389 185 9 , , PUNCT admet-389 185 10 v.gorboulev v.gorboulev NOUN admet-389 185 11 , , PUNCT admet-389 185 12 h. h. PROPN admet-389 185 13 koepsell koepsell PROPN admet-389 185 14 , , PUNCT admet-389 185 15 a. a. PROPN admet-389 185 16 sindic sindic PROPN admet-389 185 17 , , PUNCT admet-389 185 18 r. r. PROPN admet-389 185 19 schroter schroter PROPN admet-389 185 20 , , PUNCT admet-389 185 21 a. a. NOUN admet-389 185 22 zvirbliene zvirbliene PROPN admet-389 185 23 , , PUNCT admet-389 185 24 h.pavenstadt h.pavenstadt NOUN admet-389 185 25 , , PUNCT admet-389 185 26 e.schlatter e.schlatter NOUN admet-389 185 27 , , PUNCT admet-389 185 28 g.ciarimboli g.ciarimboli NOUN admet-389 185 29 . . PUNCT admet-389 185 30 am be AUX admet-389 185 31 . . PUNCT admet-389 186 1 j. j. PROPN admet-389 186 2 physiol physiol PROPN admet-389 186 3 .cell .cell PROPN admet-389 186 4 physiol physiol PROPN admet-389 186 5 . . PUNCT admet-389 187 1 290 290 NUM admet-389 187 2 ( ( PUNCT admet-389 187 3 2006 2006 NUM admet-389 187 4 ) ) PUNCT admet-389 187 5 1521–1531 1521–1531 NUM admet-389 187 6 . . PUNCT admet-389 188 1 [ [ X admet-389 188 2 3 3 NUM admet-389 188 3 ] ] X admet-389 188 4 u. u. PROPN admet-389 188 5 anand anand PROPN admet-389 188 6 , , PUNCT admet-389 188 7 a. a. PROPN admet-389 188 8 parikh parikh PROPN admet-389 188 9 , , PUNCT admet-389 188 10 m.c m.c PROPN admet-389 188 11 . . PROPN admet-389 188 12 ugwu ugwu PROPN admet-389 188 13 , , PUNCT admet-389 188 14 r. r. PROPN admet-389 188 15 u. u. PROPN admet-389 188 16 agu agu PROPN admet-389 188 17 . . PUNCT admet-389 189 1 future future PROPN admet-389 189 2 med med PROPN admet-389 189 3 . . PROPN admet-389 189 4 chem chem PROPN admet-389 189 5 . . PUNCT admet-389 190 1 6(12 6(12 NUM admet-389 190 2 ) ) PUNCT admet-389 190 3 ( ( PUNCT admet-389 190 4 2014 2014 NUM admet-389 190 5 ) ) PUNCT admet-389 190 6 1381–1397 1381–1397 NUM admet-389 190 7 . . PUNCT admet-389 191 1 [ [ X admet-389 191 2 4 4 NUM admet-389 191 3 ] ] X admet-389 191 4 a.t a.t PROPN admet-389 191 5 . . PROPN admet-389 191 6 nies nies PROPN admet-389 191 7 , , PUNCT admet-389 191 8 h. h. PROPN admet-389 191 9 koepsell koepsell PROPN admet-389 191 10 , , PUNCT admet-389 191 11 k.damme k.damme PROPN admet-389 191 12 , , PUNCT admet-389 191 13 m. m. NOUN admet-389 191 14 schwab schwab PROPN admet-389 191 15 . . PUNCT admet-389 192 1 handbook handbook PROPN admet-389 192 2 exp exp PROPN admet-389 192 3 . . PUNCT admet-389 192 4 pharmacol pharmacol PROPN admet-389 192 5 . . PUNCT admet-389 193 1 201 201 NUM admet-389 193 2 ( ( PUNCT admet-389 193 3 2011 2011 NUM admet-389 193 4 ) ) PUNCT admet-389 193 5 106 106 NUM admet-389 193 6 - - SYM admet-389 193 7 134 134 NUM admet-389 193 8 . . PUNCT admet-389 194 1 [ [ X admet-389 194 2 5 5 X admet-389 194 3 ] ] PUNCT admet-389 194 4 w. w. PROPN admet-389 194 5 m.suhre m.suhre PROPN admet-389 194 6 , , PUNCT admet-389 194 7 s.ekins s.ekin NOUN admet-389 194 8 , , PUNCT admet-389 194 9 c. c. PROPN admet-389 194 10 chang chang PROPN admet-389 194 11 , , PUNCT admet-389 194 12 p. p. PROPN admet-389 194 13 w. w. PROPN admet-389 194 14 swaan swaan PROPN admet-389 194 15 , , PUNCT admet-389 194 16 s. s. PROPN admet-389 194 17 h.wright h.wright PROPN admet-389 194 18 . . PUNCT admet-389 195 1 mol mol PROPN admet-389 195 2 . . PUNCT admet-389 196 1 pharmacol pharmacol PROPN admet-389 196 2 . . PUNCT admet-389 197 1 67 67 NUM admet-389 197 2 ( ( PUNCT admet-389 197 3 2005 2005 NUM admet-389 197 4 ) ) PUNCT admet-389 197 5 1067–1077 1067–1077 NUM admet-389 197 6 . . PUNCT admet-389 198 1 [ [ X admet-389 198 2 6 6 NUM admet-389 198 3 ] ] PUNCT admet-389 198 4 a.yonezawa a.yonezawa PROPN admet-389 198 5 , , PUNCT admet-389 198 6 k. k. PROPN admet-389 198 7 inui inui PROPN admet-389 198 8 . . PUNCT admet-389 199 1 biochem biochem PROPN admet-389 199 2 . . PUNCT admet-389 200 1 pharmacol pharmacol PROPN admet-389 200 2 . . PUNCT admet-389 201 1 81 81 NUM admet-389 201 2 ( ( PUNCT admet-389 201 3 2011 2011 NUM admet-389 201 4 ) ) PUNCT admet-389 201 5 563–568 563–568 NUM admet-389 201 6 . . PUNCT admet-389 202 1 [ [ X admet-389 202 2 7 7 X admet-389 202 3 ] ] X admet-389 202 4 m.k m.k PROPN admet-389 202 5 . . PUNCT admet-389 202 6 kim kim PROPN admet-389 202 7 , , PUNCT admet-389 202 8 c. c. PROPN admet-389 202 9 shim shim NOUN admet-389 202 10 . . PUNCT admet-389 203 1 arch arch VERB admet-389 203 2 . . PUNCT admet-389 204 1 pharm pharm NOUN admet-389 204 2 . . PUNCT admet-389 205 1 res re NOUN admet-389 205 2 . . PUNCT admet-389 206 1 29(7 29(7 NUM admet-389 206 2 ) ) PUNCT admet-389 206 3 ( ( PUNCT admet-389 206 4 2006 2006 NUM admet-389 206 5 ) ) PUNCT admet-389 206 6 605 605 NUM admet-389 206 7 - - SYM admet-389 206 8 616 616 NUM admet-389 206 9 . . PUNCT admet-389 207 1 [ [ X admet-389 207 2 8 8 NUM admet-389 207 3 ] ] X admet-389 207 4 g. g. PROPN admet-389 207 5 ciarimboli ciarimboli PROPN admet-389 207 6 , , PUNCT admet-389 207 7 k. k. PROPN admet-389 207 8 struwe struwe PROPN admet-389 207 9 , , PUNCT admet-389 207 10 p.arndt p.arndt PROPN admet-389 207 11 , , PUNCT admet-389 207 12 v. v. ADP admet-389 207 13 gorboulev gorboulev NOUN admet-389 207 14 , , PUNCT admet-389 207 15 h. h. PROPN admet-389 207 16 koepsell koepsell PROPN admet-389 207 17 , , PUNCT admet-389 207 18 e. e. PROPN admet-389 207 19 schlatter schlatter PROPN admet-389 207 20 , , PUNCT admet-389 207 21 j.r j.r PROPN admet-389 207 22 . . PROPN admet-389 207 23 hirsch hirsch PROPN admet-389 207 24 . . PUNCT admet-389 208 1 j. j. PROPN admet-389 208 2 cell cell PROPN admet-389 208 3 . . PUNCT admet-389 209 1 physio physio PROPN admet-389 209 2 . . PUNCT admet-389 210 1 201 201 NUM admet-389 210 2 ( ( PUNCT admet-389 210 3 2004 2004 NUM admet-389 210 4 ) ) PUNCT admet-389 210 5 420–428 420–428 NUM admet-389 210 6 . . PUNCT admet-389 211 1 [ [ X admet-389 211 2 9 9 NUM admet-389 211 3 ] ] PUNCT admet-389 211 4 j. j. PROPN admet-389 211 5 j.salomon j.salomon PROPN admet-389 211 6 , , PUNCT admet-389 211 7 s.endter s.endter NOUN admet-389 211 8 , , PUNCT admet-389 211 9 g.tachon g.tachon ADJ admet-389 211 10 , , PUNCT admet-389 211 11 f.falson f.falson PROPN admet-389 211 12 , , PUNCT admet-389 211 13 s. s. PROPN admet-389 211 14 t. t. PROPN admet-389 211 15 buckley buckley PROPN admet-389 211 16 , , PUNCT admet-389 211 17 c. c. PROPN admet-389 211 18 ehrhardt ehrhardt PROPN admet-389 211 19 . . PUNCT admet-389 212 1 eur eur PROPN admet-389 212 2 . . PUNCT admet-389 213 1 j. j. PROPN admet-389 213 2 pharm pharm PROPN admet-389 213 3 . . PUNCT admet-389 214 1 biopharm biopharm PROPN admet-389 214 2 . . PUNCT admet-389 215 1 81 81 NUM admet-389 215 2 ( ( PUNCT admet-389 215 3 2012 2012 NUM admet-389 215 4 ) ) PUNCT admet-389 216 1 351–359 351–359 NUM admet-389 216 2 . . PUNCT admet-389 217 1 [ [ X admet-389 217 2 10 10 NUM admet-389 217 3 ] ] X admet-389 217 4 d. d. PROPN admet-389 217 5 shao shao PROPN admet-389 217 6 , , PUNCT admet-389 217 7 e. e. PROPN admet-389 217 8 massoud massoud PROPN admet-389 217 9 , , PUNCT admet-389 217 10 u. u. PROPN admet-389 217 11 anad anad PROPN admet-389 217 12 , , PUNCT admet-389 217 13 a.parikh a.parikh PROPN admet-389 217 14 , , PUNCT admet-389 217 15 e. e. PROPN admet-389 217 16 cowley cowley PROPN admet-389 217 17 , , PUNCT admet-389 217 18 d. d. PROPN admet-389 217 19 clark clark PROPN admet-389 217 20 r.u r.u PROPN admet-389 217 21 . . PROPN admet-389 217 22 agu agu PROPN admet-389 217 23 . . PUNCT admet-389 218 1 ther ther PROPN admet-389 218 2 . . PUNCT admet-389 219 1 drug drug PROPN admet-389 219 2 del del PROPN admet-389 219 3 . . PROPN admet-389 219 4 4 4 NUM admet-389 219 5 ( ( PUNCT admet-389 219 6 2013 2013 NUM admet-389 219 7 ) ) PUNCT admet-389 219 8 1 1 NUM admet-389 219 9 - - SYM admet-389 219 10 13 13 NUM admet-389 219 11 . . PUNCT admet-389 220 1 [ [ X admet-389 220 2 11 11 NUM admet-389 220 3 ] ] X admet-389 220 4 c. c. PROPN admet-389 220 5 bosquillon bosquillon PROPN admet-389 220 6 . . PUNCT admet-389 221 1 j. j. PROPN admet-389 221 2 pharma pharma PROPN admet-389 221 3 . . PUNCT admet-389 222 1 scs scs PROPN admet-389 222 2 . . PROPN admet-389 222 3 99 99 NUM admet-389 222 4 ( ( PUNCT admet-389 222 5 2010 2010 NUM admet-389 222 6 ) ) PUNCT admet-389 222 7 2240–2255 2240–2255 NUM admet-389 222 8 . . PUNCT admet-389 223 1 [ [ X admet-389 223 2 12 12 NUM admet-389 223 3 ] ] PUNCT admet-389 223 4 ema ema PROPN admet-389 223 5 guideline guideline NOUN admet-389 223 6 on on ADP admet-389 223 7 the the DET admet-389 223 8 investigation investigation NOUN admet-389 223 9 of of ADP admet-389 223 10 drug drug NOUN admet-389 223 11 interactions interaction NOUN admet-389 223 12 , , PUNCT admet-389 223 13 cpmp cpmp NOUN admet-389 223 14 / / SYM admet-389 223 15 ewp/560/95 ewp/560/95 PROPN admet-389 223 16 / / SYM admet-389 223 17 rev rev ADJ admet-389 223 18 . . PROPN admet-389 223 19 1 1 NUM admet-389 223 20 corr corr NOUN admet-389 223 21 . . PUNCT admet-389 224 1 2 2 X admet-389 224 2 . . X admet-389 224 3 www.ema.europa.eu/docs/en_gb/document...guideline/2010/.../wc500090112.pdf www.ema.europa.eu/docs/en_gb/document...guideline/2010/.../wc500090112.pdf PROPN admet-389 224 4 ( ( PUNCT admet-389 224 5 accessed access VERB admet-389 224 6 on on ADP admet-389 224 7 april april PROPN admet-389 224 8 27 27 NUM admet-389 224 9 , , PUNCT admet-389 224 10 2017 2017 NUM admet-389 224 11 ) ) PUNCT admet-389 224 12 . . PUNCT admet-389 225 1 [ [ X admet-389 225 2 13 13 NUM admet-389 225 3 ] ] X admet-389 225 4 fda fda PROPN admet-389 225 5 guidance guidance NOUN admet-389 225 6 for for ADP admet-389 225 7 industry industry NOUN admet-389 225 8 drug drug NOUN admet-389 225 9 interaction interaction NOUN admet-389 225 10 studies study NOUN admet-389 225 11 study study VERB admet-389 225 12 design design NOUN admet-389 225 13 , , PUNCT admet-389 225 14 data datum NOUN admet-389 225 15 analysis analysis NOUN admet-389 225 16 , , PUNCT admet-389 225 17 implications implication NOUN admet-389 225 18 for for ADP admet-389 225 19 dosing dose VERB admet-389 225 20 , , PUNCT admet-389 225 21 and and CCONJ admet-389 225 22 labelling labelling NOUN admet-389 225 23 recommendations recommendation NOUN admet-389 225 24 . . PUNCT admet-389 226 1 https://www.fda.gov/downloads/drugs/guidances/ucm292362.pdf https://www.fda.gov/downloads/drugs/guidances/ucm292362.pdf PROPN admet-389 226 2 ( ( PUNCT admet-389 226 3 accessed access VERB admet-389 226 4 on on ADP admet-389 226 5 april april PROPN admet-389 226 6 27 27 NUM admet-389 226 7 , , PUNCT admet-389 226 8 2017 2017 NUM admet-389 226 9 ) ) PUNCT admet-389 226 10 . . PUNCT admet-389 227 1 https://www.fda.gov/downloads/drugs/guidances/ucm292362.pdf https://www.fda.gov/downloads/drugs/guidances/ucm292362.pdf PROPN admet-389 227 2 admet admet PROPN admet-389 227 3 & & CCONJ admet-389 227 4 dmpk dmpk PROPN admet-389 227 5 5(2 5(2 NUM admet-389 227 6 ) ) PUNCT admet-389 227 7 ( ( PUNCT admet-389 227 8 2017 2017 NUM admet-389 227 9 ) ) PUNCT admet-389 227 10 135 135 NUM admet-389 227 11 - - SYM admet-389 227 12 145 145 NUM admet-389 227 13 fluorescent fluorescent ADJ admet-389 227 14 organic organic ADJ admet-389 227 15 cations cation NOUN admet-389 227 16 for for ADP admet-389 227 17 human human ADJ admet-389 227 18 oct2 oct2 NOUN admet-389 227 19 transporters transporter NOUN admet-389 227 20 screening screen VERB admet-389 227 21 doi doi NOUN admet-389 227 22 : : PUNCT admet-389 227 23 10.5599 10.5599 NUM admet-389 227 24 / / SYM admet-389 227 25 admet.5.2.389 admet.5.2.389 NOUN admet-389 227 26 145 145 NUM admet-389 228 1 [ [ X admet-389 228 2 14 14 NUM admet-389 228 3 ] ] X admet-389 228 4 m.r m.r PROPN admet-389 228 5 koehler koehler PROPN admet-389 228 6 , , PUNCT admet-389 228 7 b. b. PROPN admet-389 228 8 wissinger wissinger PROPN admet-389 228 9 , , PUNCT admet-389 228 10 v. v. ADP admet-389 228 11 gorboulev gorboulev NOUN admet-389 228 12 , , PUNCT admet-389 228 13 h. h. PROPN admet-389 228 14 koepsell koepsell PROPN admet-389 228 15 and and CCONJ admet-389 228 16 m. m. NOUN admet-389 228 17 schmidt schmidt PROPN admet-389 228 18 . . PUNCT admet-389 228 19 cytogenet cytogenet NOUN admet-389 228 20 . . PUNCT admet-389 229 1 cell cell NOUN admet-389 229 2 genet genet NOUN admet-389 229 3 . . PUNCT admet-389 230 1 79 79 NUM admet-389 230 2 ( ( PUNCT admet-389 230 3 1997 1997 NUM admet-389 230 4 ) ) PUNCT admet-389 231 1 198–200 198–200 NUM admet-389 231 2 . . PUNCT admet-389 232 1 [ [ X admet-389 232 2 15 15 NUM admet-389 232 3 ] ] X admet-389 232 4 h. h. NOUN admet-389 232 5 motohashi motohashi NOUN admet-389 232 6 , , PUNCT admet-389 232 7 y. y. PROPN admet-389 232 8 sakurai sakurai PROPN admet-389 232 9 , , PUNCT admet-389 232 10 h. h. PROPN admet-389 232 11 saito saito PROPN admet-389 232 12 , , PUNCT admet-389 232 13 s. s. PROPN admet-389 232 14 masuda masuda PROPN admet-389 232 15 , , PUNCT admet-389 232 16 y. y. PROPN admet-389 232 17 urakami urakami PROPN admet-389 232 18 , , PUNCT admet-389 232 19 m. m. NOUN admet-389 232 20 goto goto NOUN admet-389 232 21 , , PUNCT admet-389 232 22 a. a. NOUN admet-389 232 23 , , PUNCT admet-389 232 24 fukatsu fukatsu NOUN admet-389 232 25 , , PUNCT admet-389 232 26 o. o. PROPN admet-389 232 27 ogawa ogawa PROPN admet-389 232 28 , , PUNCT admet-389 232 29 k. k. PROPN admet-389 232 30 inui inui PROPN admet-389 232 31 . . PUNCT admet-389 233 1 j j PROPN admet-389 233 2 am be AUX admet-389 233 3 . . PUNCT admet-389 234 1 soc soc PROPN admet-389 234 2 . . PUNCT admet-389 235 1 nephrol nephrol PROPN admet-389 235 2 . . PUNCT admet-389 236 1 13 13 NUM admet-389 236 2 ( ( PUNCT admet-389 236 3 2002 2002 NUM admet-389 236 4 ) ) PUNCT admet-389 237 1 866–874 866–874 NUM admet-389 237 2 . . PUNCT admet-389 238 1 [ [ X admet-389 238 2 16 16 NUM admet-389 238 3 ] ] X admet-389 238 4 e. e. PROPN admet-389 238 5 rytting rytting PROPN admet-389 238 6 , , PUNCT admet-389 238 7 j.bryan j.bryan ADJ admet-389 238 8 , , PUNCT admet-389 238 9 m. m. NOUN admet-389 238 10 southard southard PROPN admet-389 238 11 , , PUNCT admet-389 238 12 k.l k.l PROPN admet-389 238 13 . . PROPN admet-389 238 14 audus audus NOUN admet-389 238 15 . . PUNCT admet-389 239 1 biochem biochem PROPN admet-389 239 2 . . PUNCT admet-389 240 1 pharmacol pharmacol PROPN admet-389 240 2 . . PUNCT admet-389 241 1 73 73 NUM admet-389 241 2 ( ( PUNCT admet-389 241 3 2007 2007 NUM admet-389 241 4 ) ) PUNCT admet-389 241 5 891 891 NUM admet-389 241 6 – – PUNCT admet-389 241 7 900 900 NUM admet-389 241 8 . . PUNCT admet-389 242 1 [ [ X admet-389 242 2 17 17 NUM admet-389 242 3 ] ] X admet-389 242 4 m.c m.c PROPN admet-389 242 5 . . PROPN admet-389 242 6 ugwu ugwu PROPN admet-389 242 7 , , PUNCT admet-389 242 8 a. a. PROPN admet-389 242 9 oli oli PROPN admet-389 242 10 , , PUNCT admet-389 242 11 c.o c.o PROPN admet-389 242 12 . . PROPN admet-389 242 13 esimone esimone PROPN admet-389 242 14 , , PUNCT admet-389 242 15 r.u r.u PROPN admet-389 242 16 . . PROPN admet-389 242 17 agu agu PROPN admet-389 242 18 . . PUNCT admet-389 243 1 j. j. PROPN admet-389 243 2 pharmacol pharmacol PROPN admet-389 243 3 . . PUNCT admet-389 244 1 toxicol toxicol PROPN admet-389 244 2 . . PUNCT admet-389 245 1 methods method NOUN admet-389 245 2 82 82 NUM admet-389 245 3 ( ( PUNCT admet-389 245 4 2016 2016 NUM admet-389 245 5 ) ) PUNCT admet-389 245 6 9–19 9–19 NOUN admet-389 245 7 . . PUNCT admet-389 246 1 [ [ X admet-389 246 2 18 18 NUM admet-389 246 3 ] ] X admet-389 246 4 k.m k.m PROPN admet-389 246 5 . . PROPN admet-389 246 6 giacomini giacomini PROPN admet-389 246 7 , , PUNCT admet-389 246 8 s.m s.m PROPN admet-389 246 9 . . PROPN admet-389 246 10 huang huang PROPN admet-389 246 11 , , PUNCT admet-389 246 12 d.j d.j PROPN admet-389 246 13 . . PROPN admet-389 246 14 tweedie tweedie PROPN admet-389 246 15 , , PUNCT admet-389 246 16 l.z l.z PROPN admet-389 246 17 . . PROPN admet-389 246 18 benet benet PROPN admet-389 246 19 , , PUNCT admet-389 246 20 k.l k.l PROPN admet-389 246 21 . . PROPN admet-389 246 22 brouwer brouwer PROPN admet-389 246 23 , , PUNCT admet-389 246 24 x. x. PROPN admet-389 246 25 chu chu PROPN admet-389 246 26 , , PUNCT admet-389 246 27 a. a. PROPN admet-389 246 28 dahlin dahlin PROPN admet-389 246 29 , , PUNCT admet-389 246 30 r. r. PROPN admet-389 246 31 evers evers PROPN admet-389 246 32 , , PUNCT admet-389 246 33 v. v. CCONJ admet-389 246 34 fischer fischer PROPN admet-389 246 35 , , PUNCT admet-389 246 36 k.m k.m PROPN admet-389 246 37 . . PROPN admet-389 246 38 hillgren hillgren PROPN admet-389 246 39 , , PUNCT admet-389 246 40 k.a k.a PROPN admet-389 246 41 . . PROPN admet-389 246 42 hoffmaster hoffmaster PROPN admet-389 246 43 , , PUNCT admet-389 246 44 t. t. PROPN admet-389 246 45 ishikawa ishikawa PROPN admet-389 246 46 , , PUNCT admet-389 246 47 d. d. PROPN admet-389 246 48 keppler keppler PROPN admet-389 246 49 , , PUNCT admet-389 246 50 r.b r.b PROPN admet-389 246 51 . . PROPN admet-389 246 52 kim kim PROPN admet-389 246 53 , , PUNCT admet-389 246 54 c.a c.a PROPN admet-389 246 55 . . PROPN admet-389 246 56 lee lee PROPN admet-389 246 57 , , PUNCT admet-389 246 58 m. m. PROPN admet-389 246 59 niemi niemi PROPN admet-389 246 60 , , PUNCT admet-389 246 61 j.w j.w PROPN admet-389 246 62 . . PROPN admet-389 246 63 polli polli PROPN admet-389 246 64 , , PUNCT admet-389 246 65 y. y. PROPN admet-389 246 66 sugiyama sugiyama PROPN admet-389 246 67 , , PUNCT admet-389 246 68 p.w p.w PROPN admet-389 246 69 . . PROPN admet-389 246 70 swaan swaan PROPN admet-389 246 71 , , PUNCT admet-389 246 72 j.a j.a PROPN admet-389 246 73 . . PROPN admet-389 246 74 ware ware PROPN admet-389 246 75 , , PUNCT admet-389 246 76 s.h s.h PROPN admet-389 246 77 . . PROPN admet-389 246 78 wright wright PROPN admet-389 246 79 , , PUNCT admet-389 246 80 s.w s.w PROPN admet-389 246 81 . . PROPN admet-389 246 82 yee yee PROPN admet-389 246 83 , , PUNCT admet-389 246 84 m.j m.j PROPN admet-389 246 85 . . PROPN admet-389 246 86 zamek zamek PROPN admet-389 246 87 - - PUNCT admet-389 246 88 gliszczynski gliszczynski PROPN admet-389 246 89 , , PUNCT admet-389 246 90 l. l. PROPN admet-389 246 91 zhang zhang PROPN admet-389 246 92 . . PUNCT admet-389 247 1 nat nat PROPN admet-389 247 2 . . PUNCT admet-389 248 1 rev rev PROPN admet-389 248 2 . . PROPN admet-389 248 3 drug drug NOUN admet-389 248 4 discov discov NOUN admet-389 248 5 . . PUNCT admet-389 249 1 9 9 NUM admet-389 249 2 ( ( PUNCT admet-389 249 3 2010 2010 NUM admet-389 249 4 ) ) PUNCT admet-389 249 5 215 215 NUM admet-389 249 6 - - SYM admet-389 249 7 236 236 NUM admet-389 249 8 . . PUNCT admet-389 250 1 [ [ X admet-389 250 2 19 19 NUM admet-389 250 3 ] ] PUNCT admet-389 251 1 p. p. PROPN admet-389 251 2 k. k. PROPN admet-389 251 3 smith smith PROPN admet-389 251 4 , , PUNCT admet-389 251 5 r. r. PROPN admet-389 251 6 i. i. PROPN admet-389 251 7 krohn krohn PROPN admet-389 251 8 , , PUNCT admet-389 251 9 g. g. PROPN admet-389 251 10 t. t. PROPN admet-389 251 11 hermanson hermanson PROPN admet-389 251 12 , , PUNCT admet-389 251 13 a. a. PROPN admet-389 251 14 k. k. PROPN admet-389 251 15 mallia mallia PROPN admet-389 251 16 , , PUNCT admet-389 251 17 f. f. PROPN admet-389 251 18 h. h. PROPN admet-389 251 19 gartner gartner PROPN admet-389 251 20 , , PUNCT admet-389 251 21 m. m. NOUN admet-389 251 22 d. d. PROPN admet-389 251 23 provenzano provenzano PROPN admet-389 251 24 , , PUNCT admet-389 251 25 e. e. PROPN admet-389 251 26 k. k. PROPN admet-389 251 27 fujimoto fujimoto PROPN admet-389 251 28 , , PUNCT admet-389 251 29 n. n. PROPN admet-389 251 30 m. m. NOUN admet-389 251 31 goeke goeke PROPN admet-389 251 32 , , PUNCT admet-389 251 33 b. b. PROPN admet-389 251 34 j j PROPN admet-389 251 35 olson olson PROPN admet-389 251 36 , , PUNCT admet-389 251 37 d. d. PROPN admet-389 251 38 c. c. PROPN admet-389 251 39 klenk klenk PROPN admet-389 251 40 . . PUNCT admet-389 252 1 anal anal PROPN admet-389 252 2 . . PUNCT admet-389 252 3 biochem biochem PROPN admet-389 252 4 . . PUNCT admet-389 253 1 150 150 NUM admet-389 253 2 ( ( PUNCT admet-389 253 3 1985 1985 NUM admet-389 253 4 ) ) PUNCT admet-389 253 5 76 76 NUM admet-389 253 6 - - SYM admet-389 253 7 85 85 NUM admet-389 253 8 . . PUNCT admet-389 254 1 [ [ X admet-389 254 2 20 20 NUM admet-389 254 3 ] ] X admet-389 254 4 e. e. PROPN admet-389 254 5 jouan jouan PROPN admet-389 254 6 , , PUNCT admet-389 254 7 m. m. NOUN admet-389 254 8 levee levee PROPN admet-389 254 9 , , PUNCT admet-389 254 10 c. c. PROPN admet-389 254 11 denizot denizot PROPN admet-389 254 12 , , PUNCT admet-389 254 13 g. g. PROPN admet-389 254 14 d. d. PROPN admet-389 254 15 violante violante PROPN admet-389 254 16 , , PUNCT admet-389 254 17 o. o. PROPN admet-389 254 18 fardel fardel PROPN admet-389 254 19 . . PUNCT admet-389 255 1 fundam fundam PROPN admet-389 255 2 . . PUNCT admet-389 256 1 clin clin PROPN admet-389 256 2 . . PUNCT admet-389 257 1 pharmacol pharmacol PROPN admet-389 257 2 . . PUNCT admet-389 258 1 28 28 NUM admet-389 258 2 ( ( PUNCT admet-389 258 3 2014 2014 NUM admet-389 258 4 ) ) PUNCT admet-389 258 5 65–77 65–77 NUM admet-389 258 6 . . PUNCT admet-389 259 1 [ [ X admet-389 259 2 21 21 NUM admet-389 259 3 ] ] X admet-389 259 4 c. c. PROPN admet-389 259 5 macdonald macdonald PROPN admet-389 259 6 , , PUNCT admet-389 259 7 d. d. PROPN admet-389 259 8 shao shao PROPN admet-389 259 9 , , PUNCT admet-389 259 10 a. a. PROPN admet-389 259 11 oli oli PROPN admet-389 259 12 , , PUNCT admet-389 259 13 r. r. PROPN admet-389 259 14 u. u. PROPN admet-389 259 15 agu agu PROPN admet-389 259 16 . . PUNCT admet-389 260 1 j. j. PROPN admet-389 260 2 drug drug PROPN admet-389 260 3 targeting target VERB admet-389 260 4 21 21 NUM admet-389 260 5 ( ( PUNCT admet-389 260 6 2013 2013 NUM admet-389 260 7 ) ) PUNCT admet-389 260 8 97–106 97–106 NUM admet-389 260 9 . . PUNCT admet-389 261 1 [ [ X admet-389 261 2 22 22 NUM admet-389 261 3 ] ] PUNCT admet-389 261 4 c.volk c.volk NOUN admet-389 261 5 , , PUNCT admet-389 261 6 v.gorboulev v.gorboulev NOUN admet-389 261 7 , , PUNCT admet-389 261 8 a. a. NOUN admet-389 261 9 kotzsch kotzsch PROPN admet-389 261 10 , , PUNCT admet-389 261 11 t.d.muller t.d.muller NOUN admet-389 261 12 , , PUNCT admet-389 261 13 h. h. PROPN admet-389 261 14 koepsell koepsell PROPN admet-389 261 15 . . PUNCT admet-389 262 1 mol mol PROPN admet-389 262 2 . . PUNCT admet-389 262 3 pharmacol pharmacol PROPN admet-389 262 4 . . PUNCT admet-389 263 1 76 76 NUM admet-389 264 1 ( ( PUNCT admet-389 264 2 2009)275–289 2009)275–289 NUM admet-389 264 3 . . PUNCT admet-389 265 1 [ [ X admet-389 265 2 23 23 NUM admet-389 265 3 ] ] PUNCT admet-389 265 4 t.yasujima t.yasujima PROPN admet-389 265 5 , , PUNCT admet-389 265 6 k. k. PROPN admet-389 265 7 ohta ohta PROPN admet-389 265 8 , , PUNCT admet-389 265 9 k.inoue k.inoue PROPN admet-389 265 10 , , PUNCT admet-389 265 11 m. m. NOUN admet-389 265 12 ishimaru ishimaru PROPN admet-389 265 13 , , PUNCT admet-389 265 14 h. h. PROPN admet-389 265 15 yuasa yuasa PROPN admet-389 265 16 . . PUNCT admet-389 266 1 drug drug NOUN admet-389 266 2 metabol metabol PROPN admet-389 266 3 . . PUNCT admet-389 267 1 disp disp NOUN admet-389 267 2 . . PUNCT admet-389 268 1 38 38 NUM admet-389 268 2 ( ( PUNCT admet-389 268 3 2010 2010 NUM admet-389 268 4 ) ) PUNCT admet-389 268 5 715 715 NUM admet-389 268 6 - - SYM admet-389 268 7 721 721 NUM admet-389 268 8 . . PUNCT admet-389 269 1 [ [ X admet-389 269 2 24 24 NUM admet-389 269 3 ] ] SYM admet-389 269 4 t.r t.r PROPN admet-389 269 5 . . PROPN admet-389 269 6 kleyman kleyman PROPN admet-389 269 7 , , PUNCT admet-389 269 8 e.j e.j PROPN admet-389 269 9 . . PROPN admet-389 269 10 cragoe cragoe PROPN admet-389 269 11 . . PUNCT admet-389 270 1 j. j. PROPN admet-389 270 2 membr membr PROPN admet-389 270 3 biol biol PROPN admet-389 270 4 . . PUNCT admet-389 271 1 105 105 NUM admet-389 271 2 ( ( PUNCT admet-389 271 3 1988 1988 NUM admet-389 271 4 ) ) PUNCT admet-389 271 5 1 1 NUM admet-389 271 6 - - SYM admet-389 271 7 21 21 NUM admet-389 271 8 . . PUNCT admet-389 272 1 [ [ X admet-389 272 2 25 25 NUM admet-389 272 3 ] ] X admet-389 272 4 w.m w.m PROPN admet-389 272 5 . . PROPN admet-389 272 6 barendt barendt PROPN admet-389 272 7 , , PUNCT admet-389 272 8 s.h s.h PROPN admet-389 272 9 . . PROPN admet-389 272 10 wright wright PROPN admet-389 272 11 . . PUNCT admet-389 273 1 j. j. PROPN admet-389 273 2 biol biol PROPN admet-389 273 3 . . PUNCT admet-389 274 1 chem chem NOUN admet-389 274 2 . . PUNCT admet-389 275 1 277 277 NUM admet-389 275 2 ( ( PUNCT admet-389 275 3 2002 2002 NUM admet-389 275 4 ) ) PUNCT admet-389 275 5 22491 22491 NUM admet-389 275 6 - - SYM admet-389 275 7 22496 22496 NUM admet-389 275 8 . . PUNCT admet-389 276 1 [ [ X admet-389 276 2 26 26 NUM admet-389 276 3 ] ] SYM admet-389 276 4 w.lee w.lee PROPN admet-389 276 5 , , PUNCT admet-389 276 6 m. m. NOUN admet-389 276 7 reichold reichold NOUN admet-389 276 8 , , PUNCT admet-389 276 9 b.edemir b.edemir NOUN admet-389 276 10 , , PUNCT admet-389 276 11 g.ciarimboli g.ciarimboli NOUN admet-389 276 12 , , PUNCT admet-389 276 13 r.warth r.warth NOUN admet-389 276 14 , , PUNCT admet-389 276 15 h. h. PROPN admet-389 276 16 koepsell koepsell PROPN admet-389 276 17 , , PUNCT admet-389 276 18 f.the´venod f.the´venod PROPN admet-389 276 19 . . PUNCT admet-389 277 1 am be AUX admet-389 277 2 j. j. PROPN admet-389 277 3 renal renal PROPN admet-389 277 4 physiol physiol PROPN admet-389 277 5 . . PUNCT admet-389 278 1 296 296 NUM admet-389 278 2 ( ( PUNCT admet-389 278 3 2009 2009 NUM admet-389 278 4 ) ) PUNCT admet-389 278 5 1504–1513 1504–1513 NUM admet-389 278 6 [ [ X admet-389 278 7 27 27 NUM admet-389 278 8 ] ] X admet-389 278 9 h. h. PROPN admet-389 278 10 koepsell koepsell PROPN admet-389 278 11 , , PUNCT admet-389 278 12 k. k. PROPN admet-389 278 13 lips lips PROPN admet-389 278 14 , , PUNCT admet-389 278 15 c. c. PROPN admet-389 278 16 volk volk PROPN admet-389 278 17 . . PUNCT admet-389 279 1 pharm pharm PROPN admet-389 279 2 . . PUNCT admet-389 280 1 res re NOUN admet-389 280 2 . . PROPN admet-389 281 1 24 24 NUM admet-389 281 2 ( ( PUNCT admet-389 281 3 2007)1227–1251 2007)1227–1251 NUM admet-389 281 4 . . PUNCT admet-389 282 1 [ [ X admet-389 282 2 28 28 NUM admet-389 282 3 ] ] PUNCT admet-389 282 4 j. j. PROPN admet-389 282 5 müller müller PROPN admet-389 282 6 , , PUNCT admet-389 282 7 k.s k.s PROPN admet-389 282 8 . . PROPN admet-389 282 9 lips lips PROPN admet-389 282 10 , , PUNCT admet-389 282 11 l. l. PROPN admet-389 282 12 metzner metzner PROPN admet-389 282 13 , , PUNCT admet-389 282 14 r.h r.h PROPN admet-389 282 15 . . PROPN admet-389 282 16 neubert neubert PROPN admet-389 282 17 , , PUNCT admet-389 282 18 h. h. PROPN admet-389 282 19 koepsell koepsell PROPN admet-389 282 20 , , PUNCT admet-389 282 21 m. m. NOUN admet-389 282 22 brandsch brandsch PROPN admet-389 282 23 . . PUNCT admet-389 282 24 biochem biochem PROPN admet-389 282 25 . . PUNCT admet-389 283 1 pharmacol pharmacol PROPN admet-389 283 2 . . PUNCT admet-389 284 1 70 70 NUM admet-389 284 2 ( ( PUNCT admet-389 284 3 2005 2005 NUM admet-389 284 4 ) ) PUNCT admet-389 284 5 1851 1851 NUM admet-389 284 6 - - SYM admet-389 284 7 1860 1860 NUM admet-389 284 8 . . PUNCT admet-389 285 1 [ [ X admet-389 285 2 29 29 NUM admet-389 285 3 ] ] X admet-389 285 4 o. o. PROPN admet-389 285 5 zolk zolk PROPN admet-389 285 6 , , PUNCT admet-389 285 7 t.f t.f PROPN admet-389 285 8 . . PROPN admet-389 285 9 solbach solbach PROPN admet-389 285 10 j. j. PROPN admet-389 285 11 könig könig PROPN admet-389 285 12 m.f m.f PROPN admet-389 285 13 . . PROPN admet-389 285 14 fromm fromm PROPN admet-389 285 15 . . PUNCT admet-389 286 1 naunyn naunyn PROPN admet-389 286 2 schmiedebergs schmiedebergs PROPN admet-389 286 3 arch arch VERB admet-389 286 4 . . PUNCT admet-389 287 1 pharmacol pharmacol PROPN admet-389 287 2 . . PUNCT admet-389 288 1 379 379 NUM admet-389 288 2 ( ( PUNCT admet-389 288 3 2009 2009 NUM admet-389 288 4 ) ) PUNCT admet-389 288 5 337348 337348 NUM admet-389 288 6 . . PUNCT admet-389 289 1 [ [ X admet-389 289 2 30 30 NUM admet-389 289 3 ] ] X admet-389 289 4 x. x. NOUN admet-389 289 5 ming ming PROPN admet-389 289 6 , , PUNCT admet-389 289 7 w. w. PROPN admet-389 289 8 ju ju PROPN admet-389 289 9 , , PUNCT admet-389 289 10 h.wu h.wu PROPN admet-389 289 11 , , PUNCT admet-389 289 12 r.r r.r PROPN admet-389 289 13 . . PROPN admet-389 289 14 tidwell tidwell PROPN admet-389 289 15 , , PUNCT admet-389 289 16 j.e j.e PROPN admet-389 289 17 hall hall NOUN admet-389 289 18 , , PUNCT admet-389 289 19 d.r d.r PROPN admet-389 289 20 thakker thakker PROPN admet-389 289 21 . . PUNCT admet-389 290 1 drug drug NOUN admet-389 290 2 metab metab PROPN admet-389 290 3 . . PUNCT admet-389 291 1 dispos dispos PROPN admet-389 291 2 . . PUNCT admet-389 292 1 37 37 NUM admet-389 292 2 ( ( PUNCT admet-389 292 3 2009 2009 NUM admet-389 292 4 ) ) PUNCT admet-389 292 5 424 424 NUM admet-389 292 6 - - SYM admet-389 292 7 430 430 NUM admet-389 292 8 . . PUNCT admet-389 293 1 [ [ X admet-389 293 2 31 31 NUM admet-389 293 3 ] ] PUNCT admet-389 293 4 c.volk c.volk NOUN admet-389 293 5 , , PUNCT admet-389 293 6 v. v. ADP admet-389 293 7 gorboulev gorboulev NOUN admet-389 293 8 , , PUNCT admet-389 293 9 t. t. NOUN admet-389 293 10 budiman budiman NOUN admet-389 293 11 , , PUNCT admet-389 293 12 g. g. PROPN admet-389 293 13 nagel nagel PROPN admet-389 293 14 , , PUNCT admet-389 293 15 h. h. PROPN admet-389 293 16 koepsell koepsell PROPN admet-389 293 17 . . PUNCT admet-389 294 1 mol mol INTJ admet-389 294 2 . . PUNCT admet-389 295 1 pharmacol pharmacol PROPN admet-389 295 2 . . PUNCT admet-389 296 1 64 64 NUM admet-389 296 2 ( ( PUNCT admet-389 296 3 2003 2003 NUM admet-389 296 4 ) ) PUNCT admet-389 296 5 1037–1047 1037–1047 NUM admet-389 296 6 . . PUNCT admet-389 297 1 [ [ X admet-389 297 2 32 32 NUM admet-389 297 3 ] ] PUNCT admet-389 297 4 m. m. NOUN admet-389 297 5 hayer hayer PROPN admet-389 297 6 - - PUNCT admet-389 297 7 zillgen zillgen PROPN admet-389 297 8 , , PUNCT admet-389 297 9 m. m. NOUN admet-389 297 10 bruess bruess NOUN admet-389 297 11 , , PUNCT admet-389 297 12 h.b h.b PROPN admet-389 297 13 . . PROPN admet-389 297 14 , , PUNCT admet-389 297 15 nisch nisch PROPN admet-389 297 16 . . PUNCT admet-389 298 1 br br PROPN admet-389 298 2 .. .. PUNCT admet-389 298 3 j. j. PROPN admet-389 298 4 pharmacol pharmacol PROPN admet-389 298 5 . . PUNCT admet-389 299 1 136(2002 136(2002 NUM admet-389 299 2 ) ) PUNCT admet-389 299 3 829 829 NUM admet-389 299 4 -836 -836 PROPN admet-389 299 5 . . PUNCT admet-389 300 1 © © PROPN admet-389 300 2 2017 2017 NUM admet-389 300 3 by by ADP admet-389 300 4 the the DET admet-389 300 5 authors author NOUN admet-389 300 6 ; ; PUNCT admet-389 300 7 licensee licensee PROPN admet-389 300 8 iapc iapc PROPN admet-389 300 9 , , PUNCT admet-389 300 10 zagreb zagreb PROPN admet-389 300 11 , , PUNCT admet-389 300 12 croatia croatia PROPN admet-389 300 13 . . PUNCT admet-389 301 1 this this DET admet-389 301 2 article article NOUN admet-389 301 3 is be AUX admet-389 301 4 an an DET admet-389 301 5 open open ADJ admet-389 301 6 - - PUNCT admet-389 301 7 access access NOUN admet-389 301 8 article article NOUN admet-389 301 9 distributed distribute VERB admet-389 301 10 under under ADP admet-389 301 11 the the DET admet-389 301 12 terms term NOUN admet-389 301 13 and and CCONJ admet-389 301 14 conditions condition NOUN admet-389 301 15 of of ADP admet-389 301 16 the the DET admet-389 301 17 creative creative ADJ admet-389 301 18 commons common NOUN admet-389 301 19 attribution attribution NOUN admet-389 301 20 license license NOUN admet-389 301 21 ( ( PUNCT admet-389 301 22 http://creativecommons.org/licenses/by/3.0/ http://creativecommons.org/licenses/by/3.0/ PROPN admet-389 301 23 ) ) PUNCT admet-389 301 24 http://creativecommons.org/licenses/by/3.0/ http://creativecommons.org/licenses/by/3.0/ PROPN