Impaginato 21 Adv. Hort. Sci., 2021 35(1): 21­32 DOI: 10.36253/ahsc­9945 Postharvest quality of pepino melon (Solanum muricatum Aiton) as influ­ enced by NPK fertilizer rates, growing environment and storage temperature C. Mutua (*), R. Gesimba, J. Ogweno Department of Crops, Horticulture and Soils, Egerton University, P.O. Box 536, 20115 Egerton, Kenya. Key words: firmness, shelf life, total soluble solids, weight loss. Abstract: The present study evaluated the effect of NPK fertilizer (17:17:17) rates (0, 100, 200, 300 and 400 kg ha­1) on the postharvest quality of field and greenhouse grown pepino melons (Solanum muricatum Ait.) stored at room temperature (15­22°C) and at low temperature (7°C). The study was carried out in randomized complete block design with fruits from the field and greenhouse, five NPK fertilizer rates as treatments and the two storage temperatures repli­ cated three times. Data were collected on percentage fruit weight loss (PWL), total soluble solids (TSS), firmness and shelf life. Results indicated that green­ house and field grown fruits from the control and plants supplied with 100 kg NPK ha­1 had low PWL at both storage temperatures. Field grown fruits from the control stored at room temperature had the highest TSS and were firmer after 28 days of storage. Field grown fruits not supplied with fertilizer and stored at low temperature had a shelf life of 27 and 26 days in trial one and two respectively. Application of 100 kg NPK ha­1 and storage of pepino melon fruits at low temperature can be used to enhance quality and shelf life. 1. Introduction Pepino melon (Solanum muricatum Aiton) is a little­known vegetable crop which belongs to the family solanaceae. It originated from the tropi­ cal and subtropical region of Andes and is grown for its edible fruits (Heiser, 1964). Pepino melon fruits are aromatic, juicy, scented, mild sweet, and vary in size, shape and colour depending on the cultivar (Martinez­Romero et al., 2003). The fruits mature 30 to 80 days after pol­ lination and the skin is usually golden yellow with purple stripes (Nuez and Ruiz, 1996). Several studies have reported significant losses in horti­ cultural produce after harvest (Toktam et al., 2019). Such losses are caused by dehydration, decay, and physiological disorders during posthar­ vest handling. Fresh fruits and vegetables also undergo rapid transforma­ tion in nutritional and sensory quality after harvest, some of which con­ tribute to loss of market value (Ahmad and Siddiqui, 2015). The losses can (*) Corresponding author: carolmutua713@yahoo.com Citation: MUTUA C., GESIMBA R., OGWENO J., 2021 ­ Postharvest quality of Pepino melon (Solanum muricatum Aiton) as Influenced by NPK fertilizer rates, growing environment and storage tempe‐ rature. ­ Adv. Hort. Sci., 35(1): 21­32 Copyright: © 2021 Mutua C., Gesimba R., Ogweno J. This is an open access, peer reviewed article published by Firenze University Press (http://www.fupress.net/index.php/ahs/) and distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Competing Interests: The authors declare no competing interests. Received for publication 20 October 2020 Accepted for publication 27 January 2021 AHS Advances in Horticultural Science https://doi.org/10.36253/ahsc-9945 http://www.fupress.net/index.php/ahs http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ Adv. Hort. Sci., 2021 35(1): 21­32 22 be reduced through good management of pre­ and postharvest factors (Toktam et al., 2019). Postharvest quality is also affected by climatic fac­ tors such as temperature and light intensity, and other pre­harvest factors like soil type, fertilization, irrigation, mulching, and other cultural practices (Toktam et al., 2019). Temperature affects growth and development of fruits and vegetables as well as cellular compounds, their structure and this in turn affects produce firmness (Toktam et al., 2019). F e r ti l i z e r s h a v e a l s o b e e n s h o w n t o i n fl u e n c e postharvest quality of most fruits and vegetables. The type of fertilizer used and the amount applied will dictate the quality of the resulting vegetables (Arah et al., 2015). Application of potassium fertiliz­ ers on tomato has been shown to improve fruit colour, reduce the occurrence of yellow shoulder and enhance titratable acidity (Passam et al., 2007). On the other hand, application of high doses of nitroge­ nous fertilizers to greenhouse grown tomatoes reduces fruit quality by reducing total soluble solids (Passam et al., 2007). Temperature management between the time of harvesting and consumption has been shown to be effective in maintaining the quality of harvested veg­ etables. High temperatures increase metabolic activi­ ties and ethylene production but this is dependent on other factors like oxygen or carbon dioxide levels, time of exposure and the ripening stage (De Wild et al., 2003). Storage of vegetables at low temperature slows down metabolic processes and hence extends the shelf life of horticultural produce (Arah et al., 2015). The present study sought to investigate the effect of NPK fertilizer rates, growing environment and storage temperature on the postharvest quality of pepino melon. 2. Materials and Methods Experimental site description T h e e x p e r i m e n t w a s c o n d u c t e d a t t h e Horticulture Research and Teaching Field, Egerton University, Njoro. The field lies at a latitude of 0° 23’ South, longitudes 35° 35’ East in the Lower Highland III Agro Ecological Zone (LH3) at an altitude of approximately 2,238 m above sea level. Average maximum and minimum temperatures range from 19°C to 22°C and 5°C to 8°C, respectively, with a total annual rainfall ranging from 1200 to 1400 mm. The soils are predominantly mollic andosols (Jaetzold and Schimdt, 2006). The greenhouse used was 8 m by 60 m and the covering material was polythene with a thickness of 12×150 microns purchased from Amiran Kenya Ltd. The mean monthly temperatures in the greenhouse and field during the experiment are pre­ sented in Table 1. Plant material and experimental design Pepino seedlings (Ecuadorian Gold variety) were obtained from Garlic and Pepino Farm, Nakuru and planted in the field and greenhouse. The experimen­ tal design was randomized complete block design (RCBD) with fruits from the five NPK fertilizer treat­ ments, the two growing environments and two stor­ age temperatures replicated three times. The five NPK fertilizer treatments were [0, 100, 200, 300 and 400 NPK (17:17:17) kg ha­1], two growing environ­ ments (field and greenhouse) and two storage tem­ peratures (room and low temperature). Mature green pepino fruits were harvested from the field and greenhouse experiments and stored at low tem­ perature (7°C) in a refrigerator and at room temper­ ature (15­22°C) in the biotechnology laboratory of Table 1 ­ Average monthly field and greenhouse temperature (°C) in trial one and two Experimental conditions Temperature 2018 2019 Trial one Nov Dec Jan Feb Mar Apr May Field 20.9 19.7 20.9 21.7 22.8 22.6 21.2 Greenhouse 30.3 21.0 33.4 30.2 29.4 34.0 35.8 2019 2020 Trial two July Aug Sept Oct Nov Dec Jan Field 19.1 19.2 20.5 19.3 19.3 18.9 19.1 Greenhouse 18.5 29.4 30.0 28.0 32.0 28.0 35.3 Mutua et al. ‐ Pepino melon postharvest studies 23 Egerton University. The experimental design was RCBD consisting of fruits from the five treatments replicated three times. The experiment therefore comprised of five treatments similar to those of the field and greenhouse experiments each replicated three times giving a total of thirty experimental units each represented by a plastic tray. Each experimen­ tal unit comprised of twenty pepino fruits randomly selected from the harvest of the individual respec­ tive treatments in the field and greenhouse experi­ ments. Data collection and analysis Data were collected and recorded on percentage fruit weight loss (PWL), firmness, total soluble solids (TSS) and shelf life. To determine PWL, five fruits in each replication for each treatment were marked before storage and weighed using a digital balance (HANGPING JA 12002, Japan). The same fruits were weighed at the beginning of the experiment and weighing continued at an interval of 7 days for 28 days. The results were expressed as the percentage loss of initial weight using the formula: Percentage weight loss = Initial weight­final weight × 100 Initial weight Fruit firmness was determined using hand held penetrometer (model 62/DR, UK) from the begin­ ning of the experiment and continued at an interval of 7 days for 28 days. The results were reported in kg Force. TSS was determined on the same fruits used for determination of firmness using a hand held refractometer (0­30°Brix) (RHW refractometer, Optoelectronic Technology Company Ltd, UK) was used as per the procedure described by Tigchelaar (1986). Results were expressed as °Brix. This was done at the beginning of the experiment and con­ tinued at an interval of 7 days for 28 days. The shelf life of pepino fruits was determined by counting the number of days at which at least 50% of the fruits had reached senescence and were not marketable (too soft, wrinkled or with fungal rots). Quality evaluation was done using a rating scale of 1­5 (Miguel and Marita, 1996). Data collected were subjected to Analysis of Variance (ANOVA) and significant means separated u s i n g T u k e y ’ s h o n e s t l y s i g n i f i c a n t d i f f e r e n c e (Tukey’s HSD) test at p≤0.05. The SAS statistical package (SAS Institute, 2005) was used for data analysis. 3. Results Percentage weight loss (PWL) NPK fertilizer rates, growing environment and storage temperature had a significant effect at p≤ 0.05 on PWL of Pepino fruits after 28 days of storage in trial one. During this trial, highest PWL of 10.863% and 15.77% were recorded in greenhouse grown fruits from plants supplied with 300 and 400 kg NPK ha­1 during production as well as in field grown fruits from plants supplied with 400 kg NPK ha­1 regardless of the storage temperature (Table 2). The lowest PWL was, on the other hand, recorded in greenhouse and field grown fruits without NPK fertilizer applica­ tion (control) stored at low temperature although the difference in weight loss for this treatment was not significantly different from that of other treatment combinations. In trial two, NPK fertilizer rates, grow­ ing environment and storage temperature had a sig­ nificant effect on PWL from day 7 to day 28 of the study. On day 28, greenhouse and field grown fruits from plants supplied with 400 kg NPK ha­1, stored at room temperature had the highest PWL of 19.38% and 15.54% respectively (Table 2). It was noted that as the fertilizer rates increased the PWL also increased in both growing environ­ ments and storage temperatures in both trials. Generally, fruits stored at low temperature had lower PWL compared to those stored at room temperature in both trials. Greenhouse grown fruits also had a higher PWL compared to field grown fruits in both trials. Total soluble solids (TSS) NPK fertilizer rates, growing environment and stor­ age temperature had a significant effect at p≥0.05 on TSS of pepino fruits from day 7 to day 28 in both tri­ als. In trial one, field grown fruits from plants which were not supplied with fertilizer (control) and stored at room temperature had the highest TSS from day 7 to day 28. The highest TSS was recorded after 28 days of storage where field grown fruits from plants not supplied with NPK fertilizer and stored at room tem­ perature had a TSS of 8.67 °Brix which was significant­ ly higher than that recorded from fruits from all other treatment combinations. Greenhouse grown fruits from plants supplied with the highest fertilizer rate of 400 kg NPK ha­1 and stored at low temperature had the lowest TSS of 4.40°Brix after 28 days (Table 3). In trial two, field grown fruits from plants not supplied with NPK fertilizer (control) and stored at room tem­ Adv. Hort. Sci., 2021 35(1): 21­32 24 * Means followed by the same letters in a given day and trial are not significantly different according to Tukey’s Honestly Significant Difference Test at p≤0.05. Room storage temperature varied between 15 and 22°C. Low temperature was 7°C. Storage temperature Environment Fertilizer (kg ha­1) Weight loss Day 7 Day 14 Day 21 Day 28 Trial one Room temperature Field 0 1.067 2.050 2.837 3.987def 100 1.423 2.800 3.620 5.303 def 200 1.980 3.580 4.713 6.383 cdef 300 2.500 5.147 5.717 9.120 bcd 400 4.417 7.280 11.353 12.457 ab Greenhouse 0 1.213 1.840 2.820 3.413 ef 100 2.037 4.317 5.590 7.047 cde 200 2.673 5.540 7.160 8.913 bcd 300 3.167 6.400 8.720 10.863 abc 400 5.810 9.327 11.410 15.770 a Low temperature Field 0 0.523 1.043 1.283 1.557 f 100 0.917 1.720 1.997 4.300 def 200 1.057 2.170 2.763 2.310 ef 300 1.740 3.883 4.040 7.120 cde 400 2.737 5.497 8.020 15.123 a Greenhouse 0 0.543 1.087 1.630 2.173 ef 100 1.063 1.353 2.070 2.633 ef 200 1.543 2.417 2.867 4.012 def 300 2.240 2.977 5.080 3.183 ef 400 3.950 6.107 9.067 8.630 bcd Trial two Room temperature Field 0 0.940 ij 1.173 kl 1.443 kl 4.187 fghi 100 1.893 fghi 2.633 fghijk 3.123 fghij 7.100 defg 200 2.507 efgh 3.130 defghi 4.590 cdefg 8.723 cde 300 3.667 bcd 4.773 cde 5.653 cd 11.690 bc 400 4.550 b 7.363 b 10.883 b 15.543 ab Greenhouse 0 1.156 hij 1.670 ijkl 1.943 ijkl 3.360 fghi 100 3.067 cdef 3.077 efghi 4.230 defgh 7.293 def 200 3.703 bcd 4.333 cdef 5.327 cd 9.290 cd 300 4.113 bc 4.813 cd 6.190 c 11.600 bc 400 6.617 a 10.477 a 14.703 a 19.397 a Low temperature Field 0 0.663 j 0.917 l 1.283 l 1.653 i 100 1.047 ij 1.420 ijkl 1.997 ijkl 2.243 hi 200 1.970 efghi 2.173 hijkl 2.783 hijkl 2.487 hi 300 2.720 defg 2.887 fghij 3.533 efghi 3.017 ghi 400 4.127 bc 3.997 cdefg 4.740 cdef 5.967 defgh Greenhouse 0 1.607 ghij 1.337 jkl 1.720 jkl 2.190 hi 100 2.313 efgh 2.473 ghijkl 2.993 ghijk 4.593 efghi 200 3.117 cde 3.520 defgh 4.223 defgh 5.330 defghi 300 3.693 bcd 4.163 cdefg 4.823 cde 4.880 efghi 400 4.473 b 5.487 c 6.20 c 5.930 defgh Table 2 ­ Effect of NPK fertilizer rates, growing environment and storage temperature on percentage weight loss of pepino fruits in trial one and two Mutua et al. ‐ Pepino melon postharvest studies 25 Storage temperature Environment Fertilizer (kg ha­1) TSS (°Brix) Day 0 Day 7 Day 14 Day 21 Day 28 Trial one Room temperature Field 0 4.00 6.00 a 6.93 a 7.83 a 8.67 a 100 4.00 4.37 cde 5.73 b 6.13 c 6.40 bcde 200 4.00 4.53 cde 4.97 c 5.73 cd 6.13 cdef 300 4.00 4.53 cde 4.90 c 5.47 de 5.80 defg 400 4.00 4.17 de 4.67 cde 4.80 ghi 5.20 ghi Greenhouse 0 4.00 5.00 b 6.00 b 6.77 b 7.07 b 100 4.00 4.40 cde 4.90 c 5.47 de 5.80 defg 200 4.00 4.47 cde 4.90 c 5.23 ef 5.67 fgh 300 4.00 4.17 de 4.63 cde 4.86 fghi 5.10 ghij 400 4.00 4.10 e 4.30 ef 4.53 hijk 4.80 ij Low temperature Field 0 4.00 5.00 b 5.63 b 6.00 c 6.83 bc 100 4.00 4.60 bcd 4.83 c 5.13 efg 5.77 efg 200 4.00 4.43 cde 4.63 cde 4.97 fg 5.27 ghi 300 4.00 4.30 de 4.40 def 4.73 ghij 4.97 hij 400 4.00 4.10 e 4.13 f 4.33 jk 4.56 ij Greenhouse 0 4.00 4.77 bc 5.00 c 5.90 c 6.50 bcd 100 4.00 4.60 bcd 4.77 cd 4.93 fghi 5.23 ghi 200 4.00 4.47 cde 4.63 cde 4.80 ghi 4.97 hij 300 4.00 4.30 de 4.33 ef 4.50 ijk 4.73 ij 400 4.00 4.17 de 4.10 f 4.20 k 4.40 j Trial two Room temperature Field 0 4.00 5.60 a 6.70 a 7.60 a 8.133 a 100 4.00 4.50 cde 5.36 c 5.67 d 6.10 cd 200 4.00 4.33 defg 4.97 cde 5.26 ef 5.77 def 300 4.00 4.17 efg 4.53 fghi 4.80 ghi 5.00 hij 400 4.00 4.60 g 4.13 ijk 4.27 j 4.50 jkl Greenhouse 0 4.00 4.70 bc 5.23 c 6.07 c 6.50 bc 100 4.00 4.50 cde 4.80 def 5.10 efg 5.50 efgh 200 4.00 4.33 defg 4.97 cde 4.87 ghi 5.13 ghi 300 4.00 4.13 fg 4.53 fghi 4.60 ij 4.90 ijk 400 4.00 4.03 g 4.07 k 4.27 j 4.47 kl Low temperature Field 0 4.00 5.27 a 5.83 b 6.47 b 6.93 b 100 4.00 4.60 bcd 5.00 cde 5.37 de 5.83 def 200 4.00 4.30 defg 4.70 efg 4.93 fghi 5.43 fgh 300 4.00 4.13 fg 4.50 fghij 4.70 hi 5.00 hij 400 4.00 4.07 fg 4.10 jk 4.27 j 4.60 jkl Greenhouse 0 4.00 4.87 b 5.17 cd 5.67 d 6.00 cde 100 4.00 4.50 cde 4.77 defg 5.00 efgh 5.53 efg 200 4.00 4.30 defg 4.53 fghi 4.73 ghi 5.00 hij 300 4.00 4.13 fg 4.37 ghijk 4.60 ij 4.83 ijk 400 4.00 4.03 g 4.13 ijk 4.23 j 4.30 l Table 3 ­ Effect of NPK fertilizer rates, growing environment and storage temperature on TSS (°Brix) of pepino melon fruits in trial one and two * Means followed by the same letters in a given day and trial are not significantly different according to Tukey’s Honestly Significant Difference Test at p ≤ 0.05. Room storage temperature varied between 15 and 22°C. Low temperature was 7°C. perature had the highest TSS from day 14 to day 28. On day 7, field grown fruits from plants not supplied with NPK fertilizer and stored at room or under low temperature had the highest TSS of 5.6 and 5.27 °Brix, respectively (Table 3). The highest TSS was recorded after 28 days of storage where field grown fruits from plants not supplied with NPK fertilizer and stored at room temperature had a TSS of 8.13 °Brix which was significantly higher than for greenhouse grown fruits from plants supplied with 400 kg NPK ha­1 and stored 26 Adv. Hort. Sci., 2021 35(1): 21­32 at low temperature with a TSS of 4.3°Brix. It was observed that TSS increased as the storage time pro­ gressed and decreased as the fertilizer rates increased regardless of the environment under which the fruits were produced and the temperature during storage. Generally, field grown fruits had higher TSS compared to greenhouse grown fruits regardless of the storage temperatures in both trials. On the other hand, fruits stored at low temperature had lower TSS values com­ pared to those stored at room temperature in both trials. Firmness NPK fertilizer rates, growing environment and storage temperature had a significant effect on firm­ ness of pepino melon fruits on day 7 and day 28 in trial one, and day 21 and 28 in trial two. In day 7 of trial one, field grown fruits from plants which were not supplied with NPK fertilizer and stored at either room or low temperature had the highest firmness of 4.67 kg F and 4.83 kg F, respectively. However, this was not significantly different from the firmness of 4.57 kg F recorded for greenhouse grown fruits har­ vested from plants not supplied with NPK fertilizer and maintained under low temperature during stor­ age. On day 28, the highest firmness was recorded in field grown fruits from plants not supplied with NPK fertilizer and stored at low temperature with a firm­ ness of 3.83 kg F. The lowest firmness of 0.52 kg F was recorded in greenhouse grown fruits from plants supplied with 400 kg NPK ha­1 and stored at room temperature after 28 days of storage (Table 4). In trial two, NPK fertilizer rates, growing environ­ ment and storage temperature had a significant effect on firmness of pepino melon fruits at day 21 and day 28 of storage. In day 21 of storage, field grown fruits from plants not supplied with NPK fertilizer and stored at low temperature had the highest firmness of 4.13 kg F but this was not significantly different from the firmness of 3.93 kg F recorded from green­ house fruits from plants not supplied with NPK fertiliz­ er and stored at low temperature, field grown fruits from plants not supplied with NPK fertilizer stored at room temperature with a firmness of 3.70 kg F and field grown fruits from plants supplied with 100 kg NPK ha­1 maintained under low temperature with a firmness of 3.67 kg F (Table 4). The lowest firmness of 1.70 kg F was recorded in greenhouse grown fruits from plants supplied with 400 kg NPK ha­1 stored at low temperature but this was not significantly differ­ ent from the other treatment combinations. Generally, it was observed that field grown fruits were firmer compared to greenhouse grown fruits and fruits stored at low temperature were firmer compared to those stored at room temperature. Firmness also decreased as the fertilizer rates and storage days increased. Shelf life NPK fertilizer rates, growing environment and storage temperature had a significant effect (p≤0.05) on the shelf life of pepino melon fruits in both trials. In trial one, field grown pepino fruits from plants which were not supplied with NPK fertilizer (control) and stored at low temperature (7°C) had the longest shelf life of 27 days. Field grown fruits from plants supplied with 100 kg NPK ha­1 stored at low tempera­ ture had shelf life of 22 days but this was not signifi­ cantly different from the shelf life of greenhouse grown fruits from plants not supplied with NPK fertil­ izer and those supplied with 100 kg NPK ha­1 and maintained at low temperature with a shelf life of 21 and 19 days respectively (Table 5). The lowest shelf life of 11 days was recorded in greenhouse grown fruits from plants supplied with 400 kg NPK ha­1 and stored at room temperature although this was not significantly different from that of field grown fruits from plants supplied with 300 and 400 kg NPK ha­1 and stored at room temperature, greenhouse grown fruits from plants supplied with 200 and 300 kg NPK ha­1 and stored at room temperature and field grown fruits from plants supplied with 400 kg NPK ha­1 and stored at low temperature. In trial two, field grown pepino fruits from plants not supplied with NPK fertilizer and stored at low temperature had the longest shelf life of 26 days, fol­ lowed by field grown fruits from plants supplied with 100 kg NPK ha­1 stored at low temperature with a shelf life of 21 days. The shelf life recorded for fruits from this treatments was, however, not significantly different from that of greenhouse grown fruits from plants not supplied with fertilizer, those supplied with 100 kg NPK ha­1 and stored at low temperature and field grown fruits from the control and stored at room temperature (Table 5). The lowest shelf life was recorded in greenhouse grown fruits from plants sup­ plied with 400 kg NPK ha­1 stored at room tempera­ ture with a shelf life of 10 days but this was not signif­ icantly different from the shelf life recorded for green­ house grown fruits from plants supplied with 200 and 300 kg NPK ha­1 stored at room temperature and that of field grown fruits from plants supplied with 400 kg NPK ha­1 and stored at room temperature. Generally, it was observed that fruits stored at Mutua et al. ‐ Pepino melon postharvest studies 27 low temperature had a longer shelf life than those stored at room temperature. Field grown fruits had a longer shelf life compared to greenhouse grown fruits. The shelf life decreased as the NPK fertilizer rates increased. 4. Discussion and Conclusions There was a progressive increase in percentage fruit weight loss as the storage days advanced. Field and greenhouse grown fruits from plants which Table 4 ­ Effect of NPK fertilizer rates, growing environment and storage temperature on firmness (kg) of pepino melon fruits in trial one and two Storage temperature Environment Fertilize (kg ha­1) Firmness (Kg) Day 0 Day 7 Day 14 Day 21 Day 28 Trial one Room temperature Field 0 5.00 4.67 a 4.03 3.60 3.03 b 100 5.00 3.90 c 3.27 3.03 2.77 bc 200 5.00 3.57 cde 3.20 2.87 2.37 cdef 300 5.00 3.03 ef 2.57 2.00 1.57 hijk 400 5.00 2.83 f 2.33 1.50 1.02 kj Greenhouse 0 5.00 3.97 bc 3.57 2.90 2.47 cdef 100 5.00 3.73 cd 3.13 2.23 2.07 efgh 200 5.00 3.43 cdef 2.83 2.13 1.80 ghij 300 5.00 2.93 ef 2.20 1.83 1.50 ijk 400 5.00 2.17 g 1.87 1.27 0.52 l Low temperature Field 0 5.00 4.83 a 4.77 4.33 3.83 a 100 5.00 3.97 bc 3.67 3.37 2.70 bcd 200 5.00 3.83 cd 3.40 3.07 2.50 bcde 300 5.00 3.57 cde 3.20 2.83 2.17 defg 400 5.00 3.33 cdef 2.97 2.17 1.50 ijk Greenhouse 0 5.00 4.57 ab 4.40 3.30 3.03 b 100 5.00 3.53 cde 3.30 2.73 2.27 cdefg 200 5.00 3.20 def 2.93 2.40 1.93 fghi 300 5.00 3.00 ef 2.57 2.13 1.57 hijk 400 5.00 2.87 f 2.20 1.80 1.27 jk Trial two Room temperature Field 0 5.00 4.63 4.07 3.70 abc 2.73 bc 100 5.00 3.90 3.67 3.10 def 2.60 bc 200 5.00 3.80 3.17 2.87 efgh 2.13 def 300 5.00 3.43 2.87 2.17 ijk 1.87 fg 400 5.00 3.00 2.43 1.90 jk 1.20 ij Greenhouse 0 5.00 4.27 3.80 3.07 def 2.47 cde 100 5.00 3.93 3.27 2.80 fgh 2.03 efg 200 5.00 3.60 3.00 2.40 hij 1.70 fgh 300 5.00 3.13 2.63 2.00 jk 1.30 hi 400 5.00 2.90 2.20 1.70 k 0.85 j Low temperature Field 0 5.00 4.70 4.37 4.13 a 3.83 a 100 5.00 4.40 3.93 3.67 abc 2.83 bc 200 5.00 4.13 3.60 3.33 cde 2.93 b 300 5.00 3.93 3.37 3.07 def 2.63 bc 400 5.00 3.63 3.07 2.77 fgh 2.07 defg Greenhouse 0 5.00 4.27 4.07 3.93 ab 3.40 a 100 5.00 4.03 3.87 3.50 bcd 2.83 bc 200 5.00 3.83 3.20 2.97 efg 2.50 bcd 300 5.00 3.43 2.87 2.53 ghi 2.10 def 400 5.00 3.20 2.53 2.17 ijk 1.63 ghi * Means followed by the same letters in a given day and trial are not significantly different according to Tukey’s Honestly Significant Difference Test at p≤0.05. Room storage temperature varied between 15 and 22°C. Low temperature was 7°C. Adv. Hort. Sci., 2021 35(1): 21­32 28 received 400 kg NPK ha­1 and stored at room temper­ ature had the highest PWL. Similar results were reported in sweet potato in which excessive applica­ tion of nitrogen led to an increase in percentage weight loss during storage (Mark et al., 2003). Nitrogen fertilizer rates affect the rate of water loss in fruits and vegetables (Warner et al., 2004). Transpiration is the main cause of deterioration because it results in direct loss of weight. Weight loss is the major cause of softening and shriveling of fruits and vegetables damaging the appearance of fruits and loss of market value (Wilson et al., 1999). The quality of most fruits and vegetables is affected by weight loss but this depends on the temperature and humidity during storage (Perez et al., 2003). Storage of pepino fruits at room temperatures (15­22°C) could also have resulted in production of high levels of ethylene, increased respiration and subsequent weight reduction. High temperatures during storage leads to increased water loss resulting to shriveling and loss of fresh appearance of the fruits (Wills et al., 1989). Fruits lose weight when metabolic activities increase and this is accelerated by an increase in temperature around the produce resulting in loss of water. Weight loss is mainly as a result of water loss and as temperature increases the rate of water loss also increases. In this study, pepino melon fruits were harvested when green mature and as ripening progressed there was an increase in ethylene produc­ tion which led to senescence and shriveling of the fruits during storage (Wills et al., 1989). Greenhouse fruits had a higher percentage weight loss probably because of the high preharvest temperature (Table 1). At room temperature the temperatures were higher than at low temperature (7°C) and this could have resulted to faster ripening, increased respira­ tion rates and hence high PWL. Fruits stored at low temperature had lower PWL compared to those stored at room temperature. Vanitha and Mehalai (2016) also reported that pepino fruits stored at room temperature had a higher weight loss com­ p a r e d t o t h o s e s t o r e d a t l o w t e m p e r a t u r e . Temperatures above 20°C can lead to abnormal physiological processes in fresh produce, respiration occurs and water is lost to the surrounding environ­ ment and hence reduction in weight. Although there w a s a n i n c r e a s e i n P W L a s t h e s t o r a g e d a y s increased, the rate was much lower in pepino fruits stored at low temperature. Loss in weight could also b e d u e t o a c ti v i t y o f p o l y g a l a c t u r o n a s e w h i c h increases cell wall permeability and hence increase in transpiration. Low temperature reduces respiration and metabolic processes thereby slowing down the rate of fruit weight loss during storage. Low tempera­ ture also reduces the sensitivity of fruits to ethylene and senescence is reduced (Wills et al.,1989). In both trials, field and greenhouse grown pepino fruits sup­ plied with the highest NPK fertilizer rate had the highest PWL. This is in agreement with the findings of Hailu et al. (2008) and Mark et al. (2003) where appli­ cation of highest nitrogen fertilizer rates had the highest physiological weight loss of carrots and sweet potatoes during storage. The increased PWL due to increased level of nitrogen supply may be attributed to the higher moisture content in the fruits which may lead to decreased shelf­life due to rapid meta­ bolic activity, moisture loss and shrinkage in storage (El­Tantawy and El­Beik, 2009). On the other hand, fruits from the control (no NPK fertilizer) had the lowest PWL which could be attributed to low mois­ ture content in the fruits, slowed metabolic activities a n d h e n c e r e d u c e d m o i s t u r e l o s s . T h e P W L decreased as the phosphorous and potassium rates Table 5 ­ Effect of NPK fertilizer rates, growing environment and storage temperature on shelf life (days) of pepino melon fruits in trial one and two * Means followed by the same letters are not significantly diffe­ rent according to Tukey’s Honestly Significant Difference Test at p≤0.05. Room storage temperature varied between 15 and 22°C. Low temperature was 7°C. Storage temperature Environment Fertilizer (kg ha­1) Shelf life (Days) Trial 1 Trial 2 Room temperature Field 0 18 cd * 20 bcd (15­22°C) 100 17 cde 18 cdef 200 15 defg 16 fghij 300 14 fghi 14 ijk 400 12 hi 11 lm Greenhouse 0 18 cd 17 efgh 100 16 defg 14 hijk 200 13 ghi 12 klm 300 12 hi 10 lm 400 11 i 10 m Low temperature (7°C) Field 0 27 a 26 a 100 22 b 21 b 200 18 cd 17 defg 300 16 def 15 ghij 400 14 fghi 13 jkl Greenhouse 0 21 b 21 bc 100 19 bc 19 bcde 200 17 cdef 16 efghi 300 16 defg 15 ghij 400 15 efgh 14 ijk Mutua et al. ‐ Pepino melon postharvest studies 29 in the NPK fertilizer increased. This could be due to the fact that potassium plays a role in maintaining fruit firmness but high rates do not result to further increase in firmness. The firmer the fruit the less the PWL and reduction in firmness results to more PWL. On the other hand, high nitrogen levels coupled with high phosphorous levels reduce fruit quality because most of the carbohydrates are translocated to the shoots rather than to the developing fruits resulting to dense vegetative growth. Fruits produced by plants which have dense vegetative growth tend to be less firm resulting to high PWL while fruits from plants with less vegetative growth are firmer and hence low PWL. In summary, PWL progressively increased with increase in storage time in both room and low temperatures. As the fruit continues to ripen the rate of respiration also increases and this also leads to increase in weight loss. However, low tem­ p e r a t u r e l e a d s t o d e l a y e d r i p e n i n g a n d h e n c e reduced respiration resulting to low PWL compared to ambient room temperatures. TSS increased as the storage days increased. Our results are in agreement with Harman et al. (1986) and Hailu (2016) who reported that as pepino melon and mango fruits mature TSS increases significantly during maturation and ripening. The increase in TSS might be due to alteration of cell wall structure and the breakdown of complex carbohydrates into simple sugars. At room temperature, the temperatures were high and this led to an increase in metabolic process­ es, respiration and ripening resulting to high TSS. Increase in TSS could also be due to excessive mois­ ture loss of fruits which led to increased concentra­ tion of pepino fruits stored at room temperature (Nath et al., 2011). At high temperatures the rate of ripening is higher than at low temperatures and this increases TSS. Field grown pepino fruits had a higher TSS compared to greenhouse grown pepino fruits in both storage temperatures. The high TSS recorded in field grown pepino fruits could be due to high light intensity and thus high photosynthesis leading to more accumulation of sugars in the fruit compared to greenhouse grown fruits where the light intensity was low leading to reduced photosynthesis and hence low accumulation of sugars in the fruits (Beckmann et al., 2006). Any factor that interferes with photosynthesis will affect glucose and sucrose accumulation in the fruit and thus alter TSS (Rana et al., 2014). High relative humidity in the greenhouse may also have led to reduced transpiration and this enhances flow of water in the xylem vessels and this is good for the fruits because fruits act as drains for high concentrations of organic molecules leading to low water potential (Bertin et al., 2000). The low water potential in the fruits promotes absorption of water by the fruits leading to “dilution effect” making the fruits to have low TSS compared to those grown in the field (Rana et al., 2014). The low TSS recorded in greenhouse grown fruits could also be due to the fact that high temperatures during ripening of pepino melon reduce sugar content of the fruits (Pluda et al., 1993). Fruits stored at low temperature had a lower TSS compared to that of fruits stored at room tem­ perature. This could be due to delayed fruit ripening and slow conversion of carbohydrates into simple sugars. During ripening there is breakdown of com­ plex carbohydrates into simple sugars and this increase TSS. At high temperatures the conversion of carbohydrates into simple sugars is accelerated and this results to high TSS whereas at low temperature ripening is delayed and the hydrolysis of carbohy­ drates to sugars is slower, resulting to low TSS. In the present study, TSS ranged from 4.00­7.07 and 4.00­ 8.13 °Brix in trial one and two respectively. Other studies have reported lower TSS of pepino melon in the range of 4.91­5.40 °Brix (Kola, 2010) and 5.04­ 5.46 °Brix (Maruapey and Yuwono, 2016). The low TSS could be attributed to high water content in pepino fruits in the range of 90­92% (Gonzalez et al., 2000) and the fact that the quality of pepino melon fruits is greatly influenced by the environment in which these studies were conducted which is quite different from the environment in this study. TSS decreased as the fertilizer rates increased in both growing environments and storage temperatures in both trials. Field grown fruits from control plants had the highest TSS and this could be due restriction of vegetative growth because no NPK fertilizer was applied and thus the fruits became the only sink for sugars and hence increase in TSS (Pluda et al., 1993). Greenhouse grown fruits from plants supplied with the highest fertilizer rate of 400 kg NPK ha­1 had the lowest TSS and this might be due to excessive vegeta­ tive growth of both the main and side shoots there­ fore most of the photosynthates were directed to the young developing shoots rather than to the fruits leading to low sugar concentration in the fruits (Pluda et al., 1993). Excess nitrogen fertilizers make plants be more succulent, thus fruits from plants sup­ plied with 400 kg NPK ha­1 had a high water content and this might have led to dilution of sugars in the fruit resulting to low TSS. Adv. Hort. Sci., 2021 35(1): 21­32 30 Fruit firmness decreased as the storage days increased. Decrease in firmness is strongly related to increased weight loss because as the fruits lose weight they become soft hence decreased firmness. In this study, firmness decreased as the NPK fertilizer rates increased. Fruits from plants which were sup­ plied with 400 kg NPK ha­1 had the lowest firmness and this could be due to the fact that plants with dense vegetative growth are less firm than those with low or moderate vegetative growth (Toktam et al., 2019). Fruits which were not supplied with NPK fertilizer (control) were firmer due to decreased veg­ etative growth. Loss of moisture and enzymatic changes results to change in firmness (Ball, 1997). Hemicelluloses and pectins become more soluble and this causes changes and loosening of the cell wall (Paul et al., 1999). In the present study, both trials field grown pepino fruits were firmer than green­ house grown fruits. This could be due to the fact that l o w e r t e m p e r a t u r e d u r i n g t h e g r o w i n g s e a s o n increases firmness (Anagnostou and Vasilakakis, 1995). In the greenhouse the temperatures (Table 1) were high and it has been reported that high pre­ harvest temperatures tend to decrease firmness (Paul et al., 1999). Previous studies reported that loss of firmness in pepino melon is due to softening which is caused by breakdown of structural cell wall carbo­ hydrates and an increase in soluble pectic substances during storage (Heyes et al., 1994). Increase in pectic substances leads to weakening of cell walls and reduction of cohesive forces binding cells together resulting to loss of firmness (Heyes et al., 1994). In summary, fruit softening is caused by structural as well as compositional changes in various components of the cell wall carbohydrates partly as a result of fruit softening enzymes (Abbasi et al., 2011). Other studies have reported that fruit softening is as a result of cell wall digestion by pectinesterase, poly­ g a l a c t u r o n a s e a n d o t h e r e n z y m e s a n d t h i s i s increased by an increase in storage temperature (Ahmed et al., 2009). Low temperature storage main­ tained firmness of pepino melon fruits. Pepino fruits stored at low temperature had a longer shelf life compared to those stored at room temperature. This could be attributed to reduced ethylene production, respiration, ripening, weight loss, senescence, retention of firmness and reduction of other metabolic activities and this enhances shelf life and quality of produce (Lei Yi et al., 2019). On the other hand, pepino fruits stored at room tempera­ ture had a shorter shelf life because high tempera­ ture results to increased ethylene production, respi­ ration, ripening, weight loss, senescence, loss of firm­ ness and other metabolic processes and this reduced shelf life (Mutari and Debbie, 2011). Field grown fruits had a longer shelf life compared to greenhouse grown fruits. This could be attributed to lower tem­ perature in the field during the growing season (Table 1) as low temperatures have been reported to increase firmness (Anagnostou and Vasilakakis, 1995). In the greenhouse the temperatures were high and it has been reported that high temperatures t e n d t o d e c r e a s e fi r m n e s s ( P a u l e t a l . , 1 9 9 9 ) . Therefore, field grown fruits remained firmer than greenhouse grown fruits and hence the former had a longer shelf life. Fruits from the control had the longest shelf life and this could be attributed to low nitrogen levels and low water content in this fruits hence they remained firmer. On the other hand, fruits from plants supplied with high NPK fertilizer rates had a short shelf life and this might be due to high water content in the fruits due to excess nitro­ gen which also leads to postharvest decay especially fruits which were stored at room temperature. Based on the foregoing results and discussion, we conclude that application of high amounts of NPK fer­ tilizer leads to increased weight loss, less firm fruits and low TSS of pepino fruits stored at room tempera­ ture. Storage of pepino melon fruits at 7°C maintains quality through reduced weight loss and maintaining firmer fruits. We therefore recommend application of 100 kg NPK ha­1 for both field and greenhouse grown pepino melon and storage at low temperature (7°C) for enhanced quality and shelf life of the fruits. Acknowledgements The authors would like to express their sincere appreciation and thanks to World Bank through the Centre of Excellence in Sustainable Agriculture and A g r i b u s i n e s s M a n a g e m e n t ( C E S A A M ) , E g e r t o n University for the financial support of this research. Thanks to the Biotechnology Laboratory and Department of Crops, Horticulture and Soils of Egerton University, Kenya for hosting this research. References ABBASI K.S., ANJUM N., SAMMI S., MASUD T., ALI S., 2011 ­ Effect of coatings and packaging material on the Mutua et al. ‐ Pepino melon postharvest studies 31 keeping quality of mangoes (Mangifera indica L.) stored at low temperature. ­ Pak. 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