Impaginato 53 Adv. Hort. Sci., 2022 36(1): 53­62 DOI: 10.36253/ahsc­12444 Flower differentiation and fruiting dynamics in olive trees (Olea europaea): Eco­physiological analysis in the Mediterranean basin F. Maniriho (*) European University of Lefke, Lefke, Nicosia District, Cyprus. Key words: Anthesis, fruit setting, hermaphrodite and staminate flower, olive, pistil abortion. Abstract: The formation of flowers in sufficient number and quality is a prere­ quisite for a successful subsequent fruit set. Despite the abundant flowering, olive trees (Olea europea) are characterized by a very low fruit set, and a very severe yield alteration leading to market fluctuation over time. The goal of this paper is to explore and analyze eco­physiological driving factors behind the poor fruit set in Mediterranean olive groves. The key mechanisms causing floral differentiation and extreme yield alternate are functional of plant genetic varia­ bility, nutrient competition, and some ecological aspects as a response to cli­ mate change. Additionally, olive inflorescence architecture appears to be com­ plex and can vary between cultivars; the olive flower differentiation results in a variable proportion of hermaphrodite, pistillate and staminate flowers among olive cultivars as well as across canopy positions and branches, enhancing nutri­ ent competition between flowers. Self­pollination could be one of the limiting factors for increasing early fruit abscission and extreme alternate fruit­bearing. Hormonal treatments to reduce alternate production in olive trees should be explored. The current review analysis shall help to improve olive grove mana­ gement, but also for breeding new cultivars more suitable for Mediterranean agro­ecological constraints. Ovule viability and fertilisation, and embryo sac development abnormalities should all be further investigated. 1. Introduction Olive (Olea europea) farming began thousands of years ago in the Middle East and has since spread to the eastern Mediterranean; Spain and Italy produce more than 60% of the world’s olive oil (Terral et al., 2004; Herrera­Caceres et al., 2017). Some olive groves have been conver­ ted to alternative land uses, such as grazing, due to significant yearly pro­ duction fluctuation and significant competition for nutrient supplies, such as water (Loumou and Giourga, 2003). For the Mediterranean region’s delicate environment, olive grove protection is becoming a priority. Olea species are divided into four genetic groupings. According to traditional classification, three of them had close phylogenetic ties and genotypes (*) Corresponding author: festojojo@yahoo.fr Citation: MANİRİHO F., 2022 ­ Flower differentiation and fruiting dynamics in olive trees (Olea europaea): Eco‐physiological analysis in the Mediterranean basin. ­ Adv. Hort. Sci., 36(1): 53­62. Copyright: © 2022 Maniriho F. This is an open access, peer reviewed article published by Firenze University Press (http://www.fupress.net/index.php/ahs/) and distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Competing Interests: The authors declare no competing interests. Received for publication 17 December 2021 Accepted for publication 26 January 2022 AHS Advances in Horticultural Science Review paper https://doi.org/10.36253/ahsc-12444 http://www.fupress.net/index.php/ahs/ http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ Adv. Hort. Sci., 2022 36(1): 53­62 54 that were similar to wild olive genotypes (Angiolillo et al., 1999; Contento et al., 2002). Olea is a genus in the Oleaceae family with approximately twenty spe­ cies that thrive in tropical and subtropical climates throughout five continents. Based on the taxonomic position of the genus Olea and of its components, four subgenera (subg.) can be distinguished: subg. Olea section Olea, subg. Olea section Ligustroides, subg. Paniculate and subg. Tetrapilus (Besnard et al., 2002; Green et al., 2004). Based on morphology and geographical distribution, O. europea should be divi­ ded into six subspecies based on morphology and geographic distribution, including: (1) Subspecies europea, which includes the two botanical varieties, which are europea (cultivated olive) and Sylvestris (wild olive), and is widely distributed throughout the Mediterranean region; (2) Cuspidate subspecies, which is found in Southeast Asia, southern China; and the Arabian Peninsula in the east and south (Contento et al., 2002; Rugini, 2016). The O. europea L. is a frost­sensitive subtropical evergreen indigeno­ us to the Mediterranean region. It has adapted to a semi­arid temperate climate with well­drained soils, a moderate to low pH (below 8.5), and little salinity in the soil (Terral et al., 2004; Doveri and Baldoni, 2007).The olive tree is notable for its abundant bloom, which is followed by a low fruit set and a low yield. Apart from inflorescence structure, cultivars differ substantially in the proportion of a hermaphro­ dite (bisexual) to staminate flowers on inflorescences (Guevas and Polito, 2004). About approximately 10% to 15% of a mature tree’s flowers set fruit, with only 2% to 5% of them developing mature fruits, depen­ ding on the location and type (Reale et al., 2006). Low fruit set and the shift from non­functional her­ maphrodite flowers to fully functional staminate flo­ wers seems to be the most important and limiting factors for olive tree productivity. The formation of functional staminate rather than entirely functional hermaphrodite flowers throughout development is one of the primary factors determining the fruit set­ ting level of olive flowers (Reale et al., 2009). Although various studies have been done on the dif­ ferentiation process of olive flowers, there have been very few specialised investigations to document and compare biological flower development and fruit set research among cultivars in this area. Olive farming has been an important part of Mediterranean nutriti­ on, therapeutic body care, economics, and religious rites for millennia (Angiolillo et al., 1999; Rugini, 2016; FAO, 2020). The olive fruit is eaten and pressed for its seed oil, which has been shown to have health­ promoting properties when consumed regularly and can be stored and consumed for up to three years if stored properly (Besnard and Bervillé, 2000). The oil is used to make soaps, hair conditioners, massage oils, and other therapeutic products. In terms of nut­ ritional value, as well as economic importance to national economies, they are a necessary food. Olive fruits have been demonstrated to aid in the preventi­ on of coronary heart disease and various cancers due to their high level of monosaturated fatty acids and phenolic compounds (Terral et al., 2004). Despite the fact that olives have been cultivated for many years and various cultivars have been domesticated for their fruit quality, morphological and physiological properties, the Mediterranean region’s cultivar selec­ tion has not fully responded adequately to alternate fruiting and poor terminal fruit sets (Connor et al., 2014). 2. Physiological factors driving olive fruit setting Pistil abortion during olive flowering The production of functional staminate flowers rather than completely functional hermaphrodites, which are unable to yield fruit, is one of the primary reasons limiting fruit set in olive (Olea europaea L.) (Reale et al., 2009; Newton et al., 2014). Despite their extensive history, many key questions surroun­ ding olives remain unanswered. While there are up to 2,600 different olive cultivars (Rugini and Lavee, 1992), many studies on diversity within O.europea have focused on morphology and agronomic behavi­ or, with little research on variety within the Olea germplasm to yet. Low fruit set is frequent and varies between olive cultivars (Newton et al., 2014). Pollen flow (Guitian, 2006), resource availability (Terral et al., 2004), predation, environmental stress, or gene­ tic stress are all possible causes (Newton et al., 2014). The olive tree has a low ultimate fruit set due to a high rate of undeveloped pistils later leading to its abscission (Chiappetta et al., 2015). The physiolo­ gical differentiation processes are driven by competi­ tion for resources between growing vegetative and reproductive organs (Dixon, 2012; Erel, 2016). According to cytohistological observations of stami­ nate and hermaphrodite flowers, after the megaspo­ re mother cell develops, the pistil development in staminate flowers is halted (Guitian, 2006; Chiappetta et al., 2015). Biochemical studies demons­ Maniriho ‐ Dynamics of olive trees flowering and fruit set in Mediterranean basin 55 trated that starch granules were only discovered in the ovary, pistil, and stigma of hermaphrodite flo­ wers at this time. The pistils of staminate flowers did not contain any substantial amounts of starch (Wiens et al., 1987; Seifi, 2015). The findings reveal a subs­ tantial connection between starch content and pistil formation (Chiappetta et al., 2015). The low chlo­ rophyll content of the gynoecium, the absence of Rubisco activity in the pistils of these two flower types, and the ultrastructure of the plastids discove­ red by transmission electron microscopy research all point to a secondary source of starch within the flo­ wer(Reale et al., 2009; Erel, 2016). In olive varieties, the percentage of hermaphrodite to staminate flo­ wers is also significant and varies (Cuevas and Polito, 2004). Fruit success appears to affect the gender of flowers in distal positions along the inflorescence, as seen by the gender pattern of flower buds in inflores­ cences with varying fruit placements. Other aspects of andromonoecy in Caesalpinia species, such as flo­ ral sex ability and fruit set impacts, are examined (Terral et al., 2004; Seifi, 2015). Previous research used potency spectral analysis to look for all possible periodic patterns in yield data; and concluded that even with a fairly regular biennial succession, two or more “on” or “off” years can be observed. Staminate flowers are produced by andromonoecious species to boost reproductive success by increasing male functi­ on or redirecting resources away from useless pistils and onto fruits (Huang, 2003). Biennial fruit‐bearing in olive trees Alternate fruiting and determining factors are cri­ tical in olive trees. Olive crop productivity has chan­ ged greatly over the years, due to the problem of alternating fruit­bearing that has been affecting the trade and consumption in the olive producing coun­ tries. Many fruit trees, such as olive trees, may not yield the same crop year after year, with mild to sig­ nificant annual variations (Rallo et al., 1993; Seifi, 2015). In recent decades, the issue of alternating bearing in fruit trees has received a lot of attention, because of the huge swings in production, the alter­ nating bearing is a common phenomenon in many fruit tree species, causing tremendous labor, marke­ ting, and economic instability (Fig. 1). The concept of “alternate” or “biannual” bearing is defined as the production of heavy fruit “in” one year, followed by light or no fruit the next year (Monselise and Goldschmid, 1982). The development of trust worthy metrics to quantify biannual alternation, its severity (maximum and average deviation from multi­year norms), synchrony at different sites, and other cha­ racteristics are clearly of importance. Monselise and Goldschmid (1982) presented two parameters for evaluating fruit production yearly alternation). The first parameter “B” denotes the proportion of bienni­ al, while the second one “I” denotes the amplitude of volatility. Three aspects of olive reproductive biology were investigated by Rallon et al. (1993): the biannu­ al cycle, the effect of cultivation on successive repro­ ductive and vegetative processes, and the effect of temperature on bud dormancy are all discussed. Even though appropriate horticultural techniques such as sufficient pruning, thinning, irrigation, etc. are implemented, olive trees will gradually alternate their annual yield. Under good climate circumstances in the whole year, the alternate bearing will be very reduced at a given extent depending on cultivars (Lavee, 2007). A quantitative study relating cultivati­ on to various indices of successive reproductive and vegetative processes revealed that while inhibition of floral induction by developing plentiful fruits is the main factor for biennial bearing, the successive rep­ roductive processes are also important, especially fertilisation stage (Lavee and Avidan, 1993). Rosati et al. (2011) also investigated the effect of nutritional conditions on olive biennial fruiting. The researcher concluded that Flowers induction particularly her­ maphrodite flowers formation are negatively affec­ ted by flowering load (Erel et al., 2016). Furthermore, biochemical studies have been conducted in an attempt to explain the alternate bearing in olive pro­ duction. The protein content and their composition of “on” and “off” olive trees, especially the protein content in the leaves and bark of one­year­old sho­ ots, have a critical effect on the olive annual produc­ Fig. 1 ­ Variability of olive world production over 10 years. Adapted from FAOSTAT (FAO, 2020). Adv. Hort. Sci., 2022 36(1): 53­62 56 and that genetic differences in pistil abscission bet­ ween olive cultivars can be explained by changes in pistil mass and sink strength (Zilberman et al., 2007; Song et al., 2014). Addionnaly, flower bud induction is a long­term process in the olive tree that is control­ led by a variety of internal and external stimuli. Marone and Fiorino (2010) conducted experiments to identify the meristems that give birth to various types of shoots, as well as the fundamental mecha­ nisms influencing the evolution of the apical meris­ tem and its lateral buds. Observations led to the dis­ covery of a vertical succession of two types of buds in the same bud complex: the “main” bud and, in the upper position, the “accessory” bud, the former having a reproductive function and the latter speciali­ zed in environmental exploitation (vegetative role). They concluded that the generation of new bearing vegetation is confined to the central leader in “matu­ re” shoots, and all branches arising from accessory buds are committed to burst new vegetation. The fin­ dings by Fabbri and Benelli (2000) also backed the theory of the two­step induction leading to flower bud differentiation, which appears to begin around the end of fall. On the otherhand, the impact of sea­ sonal variations in the phenolic content of olive culti­ var’s leaves (Olea europaea L) on the cultivar’s alter­ nating bearing have been suggested to be associated to the alternatate bearing too. In 2008 (off year), Mert et al. (2013) reported a substantial variations in the amount and distribution of these phenolics in the leaves (on year). Chlorogenic and p­coumaric acids were abundant in the “on” year, but other phenolic compounds were scarce. The chlorogenic and p­cou­ maric acid levels were low during the “off” year, while the levels of the other phenolic acids were high. In the “on” and “off” years, the Same Authors discovered a negative connection between chloroge­ nic acid and caffeic acid concentrations: caffeic acid levels were high, while chlorogenic acid levels were low. Comparing the “on” and “off” years, they con­ cluded that the contents of chlorogenic acid, caffeic acid, 3­h ydroxycinnamic acid, and p­coumaric acid were considerably different. Ovary abortion and incomplete embryo development The findings back up the theory that pistil aborti­ on is linked to ovaries competing for resources, and they imply that genetic variations in pistil abortion between olive cultivars might be explained by chan­ ges in pistil mass and sink strength (Ji et al., 2010; Rosati et al., 2011). In andromonoecious species, pis­ tion (Lavee and Avidan, 1993; Eris et al., 2007), in Koronaiki, Uovo de Piccione, Manzanillo, and Barnea olive cultivars. The overall amount of useable protein in the “off” trees’ leaves was substantially lower than in the “on” plants’ leaves, while the bark revealed an antithetical relationship. The Koronaiki cultivar was the least alternative, presenting the smallest variance in its leaves. In contrast, other cultivars had a compa­ rable percentage of proteins content in their bark. Additionally, Cuevas and Polito (2011) observed that the “off” trees of all cultivars had more crude protein in their bark than the “on” trees of the same culti­ vars. A 66 crude protein was more expressed in the bark of the “off” trees, rather than “on” years. However, Rosati et al. (2011), found that differences in some proteins were smaller in the leaves of “on” and “off” trees (Rosati et al., 2011). Endo‐genetic factors The olive crop (Olea europaea) is a genetically diverse fruit in horticulture (Kour et al., 2018). Cytosine methylation is an essential epigenetic regu­ lator of transposon silencing, heterochromatin orga­ nization, genomic imprinting, and gene expression, according to an investigation of endogenous factors (Zhang et al., 2006). Thus, all flowers in andromomo­ nocioeus plants are claimed to be bisexually started, despite the presence of spatial patterns within inflo­ rescences and plants (Hamanishi and Campbell, 2011). In addition to the biochemical difference, Cuevas and Polito (2004) confirmed that the dry weight of hermaphrodite flowers was 19% larger than the dry weight of staminate blooms begun at similar places on the panicle. Because there were no significant differences in stamen weight, the author hypothesized that this discrepancy was mostly attri­ butable to pistil and petal weight. Delph (1997), on the other hand, found no significant differences bet­ ween staminate and hermaphrodite flowers in pollen amount per anther or pollen quality, as evaluated by viability, germination, and ability to fertilize other flo­ wers. Furthermore, no link between gender and ant­ hesis timing was discovered. On the other hand, Sedgley and Griffin (2013), found that the flower’s position inside the panicle was linked to anthesis timing and gender. The Blooms on the tip and major pedicels were hermaphrodite and opened first, whilst flowers on subsidiary pedicels were mostly staminate and achieved anthesis last. In summary, the majority of the findings support the notion that pistil abscissi­ on is linked to resource competition among ovaries, Maniriho ‐ Dynamics of olive trees flowering and fruit set in Mediterranean basin 57 til abortion is thought to be an evolutionary adaptati­ on to save resources by balancing the quantity of pis­ tils with the resources available. As a result, pistil abortion is likely to be higher in large­fruited varieti­ es. Rosati et al. (2011) working with olive cultivars with varying ovary/fruit mass discovered that pistil abortion, represented as a percentage of staminate flowers, was positively associated with the average ovary mass at bloom. Furthermore, both ovary mass and pistil abortion were inversely linked with the number of perfect flowers per inflorescence, where­ as both factors increased the number of staminate flowers per inflorescence (Famiani et al., 2019) (Fig. 2). Thus, the above researchers, among others, sug­ gested that the leaf­bud ratio, as well as the amount of leaves present for each inflorescence bud, are two factors that influence pistil abortion in olives (Fig. 2). The olive flowers will develop poorly if the number of leaves decreases; and the number of aborted pistils will rise as the number of leaves decreases. 3. Ecological factors Nutrients resources distribution and plant nutrition factors A variety of alterations in the activation and inhi­ bition of endogenous metabolic pathways are invol­ ved in the manifestation of alternative bearing. Pistillate abortion, known as andromonoecy, which refers to the generation of both perfect (hermaphro­ ditic) and staminate blooms, is thought to be influen­ ced by resource competition (Haberman, 2019). According to Rallo et al. (1993) and Cuevas and Polito (2011), the high variation in the proportion of stami­ nate flowers observed in olive trees, branches, sho­ ots, and even inflorescences within the same shoot, could be part of a general reproductive strategy that adjusts maternal investment in gender expression in response to available resources and environmental conditions. If nutrient shortages cause increased pis­ tillate abundance and staminate flower develop­ ment, Solomon (1985) and Emms (1993) suggest that the nutrition deficiency could also alter pollen output or pollen quality. If this is the case, staminate flowers would be regarded as a result of nutritional defici­ ency, and andromonoecy would be regarded as a process of partial flower abortion (Lavee and Avidan, 1993; Cuevas and Polito, 2011). In contrast, if stami­ nate flowers benefit from the resources conserved by pistillate miscarriage, we should expect more pollen grains or higher pollen performance from staminate flowers than from hermaphrodite flowers (Song et al., 2012). Vining et al. (2012) and Guevas and Polito (2013) investigated the synthesis and use of car­ bohydrates linked with regular and alternation frui­ ting in the olive production cycle. Male flowers are more likely to appear on the less fed secondary pedi­ cels, where fruit set is unlikely to happen; while her­ maphrodite flowers are more likely to form on the apex and major pedicel of the inflorescences. However, some studies have found that flower positi­ on has no effect; for example, in the ‘Mission culti­ var’, the fate of a certain floral meristem is not mixed, and gender cannot be firmly attributed to a certain inflorescence location (Delph, 1984; Fig. 2 ­ Illustration of some olive flowers development biological stage by a Light microscope: A longitudinal view of hermaphrodite flower before anthesis (i), A hermaphodite flower with a degenerating (ii), an aborted pistil (iii), a transverse view of an ovary expansion with one functional ovule and 3 others degenerating (iv) Electron micrograph of the phytoprotective film of Chi­Pyro­ Film, after spraying on a smooth surface at a temperature of 18 to 25°C (a and b). Chitosan strawberry leaf covered with a film formed by Chi­Pyro­Film (c). 58 Adv. Hort. Sci., 2022 36(1): 53­62 Haberman, 2019). Despite the fact that staminate flowers originate in less desirable locations, the findings showed that the conditions that cause pistil abortion have no effect on their function. In fact, Nitrogen tended to improve blooming intensity but not floral quality; nevertheless, it consistently reduced fruit set. The amount of phosphorus in the soil was linked to the creation of beautiful blooms and fruit set. Potassium supplementation had a minor influence on olive yield (Erel et al., 2013). Pollen grains from staminate flo­ wers, on the other hand, do not profit from the transfer of resources saved by pistil abortion, as Emms (1993) discovered. Because sexual reproducti­ on resources are limited, it is sometimes argued, on theoretical grounds, that an increase in resources given to male function comes at the expense of reso­ urces dedicated to female function, and vice versa (Delph, 1984). Furthermore, Guevas and Polito (2003) observed that in hermaphroditic flowers, lar­ ger resource allocation to pistils occurs, while stamen dry weight does not rise in staminate flowers in res­ ponse to reduced pistil allocation. The positive asso­ ciation discovered in hermaphroditic flowers betwe­ en petal, stamen, and pistil dry weight shows that conditions that favor pistil development also favor resource investment in stamens and petals (Lavee and Avidan, 1993).Plants do not shift resources from the pistil to the stamens in staminate flowers, accor­ ding to patterns of resource allocation to floral organs in other andromonoecious species (Lavee and Avidan, 1993; Emms, 1993). Reale (2009) showed that nutritional deficits can impact pollen production or pollen quality, in addition to increased pistil abor­ tion and generation of staminate flowers. If this is the case, staminate flowers would be regarded as a result of nutritional deficiency, while andromonoecy would be regarded as a simple process of incomplete floral abscission. Even though, staminate flowers gain from the resources saved by pistillate miscarriage (Song et al., 2012), we should expect more pollen grains or greater pollen performance from staminate flowers compared to hermaphrodite flowers. In this situation, the ailment could be viewed as a precursor to monoeciousness (Vining et al., 2012). During the annual and bennial cycles, there are claimed to be dramatic changes in the carbohydrate components of leaves. Sugars and starches are substantially higher at the start of a bearing year than at the start of a non­bearing year, according to Duyvelshoff (2011), polysaccharides are extensively hydrolyzed througho­ ut winter. Low temperatures and good flower induc­ tion seem to be linked to a high carbohydrate con­ tent (Rosati et al., 2006). The influence of seeds on floral induction in growing fruits has also been under­ lined. Seed­produced auxin has been seen moving from the seed to the fruit spur. In a biennial cultivar (Laxton’s Superb), the mobility is larger than in a regular bearing cultivar (‘Cox’s Orange Pippin’) (Zhang, 1993). Long ago, it was suggested that seeds may deprive a key metabolite essential for flower ini­ tiation (Duyvelshoff, 2011). The function of nutrition in pitillate abortion in olives revealed that high leaf/flower ratios and nitrogen fertilization increase hermaphroditic flower formation, which is consistent with feminization trends in andromonoecious plants developing under favorable climatic conditions (Solomon, 1985; Rosati et al., 2006). As a result, the wide range of staminate flower proportions observed in olive across years, trees, branches, shoots, and even inflorescences within the same shoot could be part of a general reproductive strategy that adjusts maternal investment in sex expression in response to available resources and environmental conditions (Rosati et al., 2006). The latter stated that high amo­ unts of metabolites or photosynthates may be asso­ ciated with high hormone levels, which is consistent with prior findings by Durand (1990) and Frankel and Galun (2012), who determined that hormone con­ centration and sex expression in plants had a close relationship. Female flowers grow near younger lea­ ves, which have high auxin levels, according to the same authors. The favorable effect of phosphorous on female reproductive development was indepen­ dent of total carbohydrate availability, according to Erel (2016) in his study on the influence of phospho­ rous nutrient levels on reproductive development. As a result, the researcher hypothesized that Phosphorous nutrition had a favorable influence on productivity measures that was unrelated to car­ bohydrate reserves or carbohydrate transit to the developing inflorescence. In addition, Fernández (2009) and Reale (2009) discovered that phosphoro­ us nutrient levels were connected to the rate of rep­ roductive bud burst, inflorescence weight, rate of hermaphrodite flowers, pistil weight, fruitlet persis­ tence, fruit set, and the overall number of fruits pro­ duced. Pollen viability was consistently high in Phosphorus deficient trees, the authors reported, presumably due to higher carbohydrate availability, Maniriho ‐ Dynamics of olive trees flowering and fruit set in Mediterranean basin 59 in contrast to female reproductive organs. Environmental factors According to ecophysiology research, the degree of alternance bearing in fruit production is strongly reliant on environmental variables and can vary signi­ ficantly between growing regions depending on cli­ mate (Terral et al., 2004; Fernández, 2015). A rese­ arch done on the influence of climatic conditions on the ratio of hermaphrodite to male flowers and fruit set, in regular and alternating olive varieties, found out that different inductive circumstances are crea­ ted by harsh climate conditions. In seasons with a lot of blooms, staminate flowers are plentiful and the number of flowers per inflorescence is fairly low (Erel et al., 2013; Erel at al., 2016).Even among the same cultivar at different locales, the effects of tree age have been studied as debatable and presumably not uniform (Song et al., 2014). The alternation pheno­ mena can be triggered by environmental factors. Because of its self­sustaining features, cyclic activity can last for years after it is started (Emms, 1993). Different tree species, as well as the same tree speci­ es cultivated in various climates, may have different relevant conditions. The same tree species can be cultivated in a variety of environments, such as irriga­ ted vs. arid culture, somewhat warm and humid vs. hot and dry summers, overcast vs. bright days, and so on. Conditions that do not trigger in one zone may become triggers in another or for different trees wit­ hin the same zone (Hamanishi and Campbell, 2011). Monselise and Goldschmid (1982) investigated the impact of plant size and light intensity interactions on sex expression. The percentage of female and her­ maphrodite flowers was highest when large plants were exposed to full sunshine. That is, when high light intensity was combined with a big plant size, the percentage of female and hermaphrodite flowers was larger than when each component was used alone (Karapatzak et al., 2012). Furthermore, it was discovered that both high light intensities and plant size increase female flowering. “Plants that are culti­ vated in full sunlight, with appropriate hydration, and embedded in an appropriate substrate are sturdy and produce female flowers in the majority of cases. Male flowers are produced by less vigorous plants that are frequently planted in the shade or lack eno­ ugh nutrients (Fernández et al., 2009). When the olive is cultivated in a greenhouse at a minimum tem­ perature of 16°C and a maximum temperature of 27­ 30°C, flower production is absolutely suppressed, alt­ hough it does occur when cultivated in California during the winter. He also came to the conclusion that morphological alterations in the bud are linked to an increase in blooming, which is regulated by the treatment time (Terral et al., 2004).The classic exam­ ple of the change in woody plants throughout a large climatic area relates to climatic factors that can trig­ ger the biennial cycle. Spring frosts in deciduous trees, for example, or unusual drought stress during the set in warm locations, are examples of such trig­ gers. Outside of the cycle, however, it is normal for woody plants and trees to swap places with their neighbors. Individual branches could even be out of sync with the rest of the tree (Monselise and Goldschmidt, 1982). To some extent, the effects of temperature on flowering, fruit set, and fruit deve­ lopment have been examined. Due to influences on pollen germination and pollen tube expansion in the fertilization process, high day and night temperatures (300oC/200oC) during the growing season might impair yield potential, resulting in flower death follo­ wing anthesis (Karapatzak et al., 2012; Haberman, 2019). 4. Conclusions Despite the fact that olive farming has been prac­ ticed in the Mediterranean agricultural ecosystem since ancient time, the productivity of some olive groves is hampered by the floral divergence leading to the low fruit set. On one hand, the pistil abscissi­ on can be viewed as an evolutionary response in the Mediterranean ecology that balances pistil numbers with available resources. On the otherhand, benefit of developing staminate flowers in olive trees can also be seen as adaptative strategy to increase male flowers activity and dispersal efficiency, as well as to boost pollinator attraction. Thus, in olive trees, the transition from hermaphrodite to staminate flowers can be regarded as a strategy to maximize the ability of male flowers by allocating biochemical plant reso­ urces to male and female tasks in the most efficient way possible. Pollination fitness and additional reso­ urces are provided for the reduced number of pistil growth, if the optimal number of staminate flowers exceeds the number of pistils. However, further rese­ arch should be done on how to balance the ecologi­ cal flower adapatability and the olive productivity. Thus, there is a needto adjust Fthe variability of the ratio between hermaphrodite and staminate flowers, Adv. Hort. Sci., 2022 36(1): 53­62 60 as well as the histological structural study that goes along with it. Hormonal treatments to reduce alter­ nance production in olive trees should be also explo­ red. Ovule viability and fertilisation, as well as embryo sac development during flower differentiati­ on and fruit abscission, should all be further investi­ gated too. Lastly, cross­effect pollination’s on fruit value between olive cultivars, as well as the synchro­ nization of flowering and fruit set, in the Mediterranean ecology could be also of great inte­ rested to improve olive productivity. Acknowledgements Special appreciation to all European University academic personnel and to the field technicians at the EUL research farm for their valuable assistance. Speciall Thanks also to University of Rwanda, for a study leave and financial support during the stay at EUL. References ANGİOLİLLO A., MENCUCCİNİ M., BALDONİ L., 1999 ­ Olive genetic diversity assessed using amplified fragment length polymorphisms. ­ Theor. Appl. Gen., 98(3­4): 411­421. BESNARD G., BERVİLLÉ A., 2000 ­ Multiple origins for Mediterranean olive (Olea europaea L. ssp. europaea) based upon mitochondrial DNA polymorphisms. ­ Comptes Rendus de l’Académie des Sciences­Series III­ Sciences de la Vie, 323(2): 173­181. CHİAPPETTA A., BRUNO L., MUZZALUPO I., 2015 ­ Omics approaches for the characterization and valorisation of olive varieties. ­ Agricultural and food biotechnology of Olea europaea and stone fruits. Bentham, Sharjah, pp. 75­108. CONNOR D.J., GÓMEZ­DEL­CAMPO M., ROUSSEAUX M.C., SEARLES P.S., 2014 ­ Structure, management and pro‐ ductivity of hedgerow olive orchards: A review. ­ Sci. Hortic., 169: 71­93. CONTENTO A., CECCARELLİ M., GELATİ M., MAGGİNİ F., BALDONİ L., CİONİNİ P., 2002 ­ Diversity of Olea genotypes and the origin of cultivated olives. ­ Theor. Appl. Gen., 104(8): 1229­1238. CUEVAS J., POLİTO V.S., 2004 ­ The role of staminate flo‐ wers in the breeding system of Olea europaea (Oleaceae): an andromonoecious, wind‐pollinated taxon. ­ Annals of Botany, 93(5): 547­553. DELPH L.F., JOHANNSSON M.H., STEPHENSON A.G., 1997 ­ How environmental factors affect pollen performance: ecological and evolutionary perspectives. ­ Ecology, 78(6): 1632­1639. DİXON G.R., 2012 ­ Climate change‐impact on crop growth and food production, and plant pathogens. ­ Canadian J. Plant Path., 34(3): 362­379. DOVERİ S., BALDONİ L., 2007 ­ Olive, pp. 253­264. ­ In: KOLE C. (ed.) Fruits and Nuts. Volume IV. Genome map‐ ping and molecular breeding in plants. Springer­Verlag, Berlin, Heidelberg, Germany, pp. 370. DURAND R., DURAND B., JACOBS M., 1990 ­ Sexual deter‐ mination and sexual differentiation. ­Critical Reviews in Plant Sci., 9(4): 295­316. DUYVELSHOFF C., 2011 ­ Plant bioregulator strategies to alleviate biennial bearing, enhance precocity, and con‐ trol vegetative growth of ‘Northern Spy’ apple trees ­ PhD Thesis, http://hdl.handle.net/10214/2679. EMMS S.K., 1993 ­ Andromonoecy in Zigadenus paniculatus (Liliaceae): spatial and temporal patterns of sex alloca‐ tion. ­ Amer. J. Bot., 80(8): 914­923. EREL R., YERMİYAHU U., VAN OPSTAL J., BEN­GAL A., SCHWARTZ A., DAG A., 2013 ­ The importance of olive (Olea europaea L.) tree nutritional status on its produc‐ tivity. ­ Sci. Hortic., 159: 8­18. EREL R., YERMİYAHU U., YASUOR H., CHAMUS D.C., SCHWARTZ A., BEN­GAL A., DAG A., 2016 ­ Phosphorous nutritional level, carbohydrate reserves and flower quality in olives. ­ PloS one, 11(12). ERİS A., GULEN H., BARUT E., CANSEV A.S.U.M.A.N., 2007 ­ Annual patterns of total soluble sugars and proteins related to coldhardiness in olive (Olea europaea L. ‘Gemlik’). ­ J. Hortic. Sci. Biotech., 82(4): 597­604. FABBRİ A., BENELLİ C., 2000 ‐ Review article flower bud induction and differentiation in olive. ­ J. Hortic. Sci. Biotech., 75(2): 131­141. FAMİANİ F., FARİNELLİ D., GARDİ T., ROSATİ A., 2019 ­ The cost of flowering in olive (Olea europaea L.). ­ Sci. Hortic. , 252: 268­273. FAO, 2020 ­ FAOSTAT. Food and agriculture data, 2020. ‐ Food and Agricultural Organization of the United Nations, Rome, Italy. FERNÁNDEZ F.J., LADUX J.L., SEARLES P.S., 2015 ­ Dynamics of shoot and fruit growth following fruit thinning in olive trees: same season and subsequent season res‐ ponses. ­ Sci. Hortic., 192: 320­330. FERNÁNDEZ­ESCOBAR R., MARİN L., SÁNCHEZ­ZAMORA M.A., GARCÍA­NOVELO J.M., MOLİNA­SORİA C., PARRA M.A., 2009 ­ Long‐term effects of N fertilization on cropping and growth of olive trees and on N accumula‐ tion in soil profile. ­ European J. Agron., 31(4): 223­232. FRANKEL R., GALUN E., 2012 ­ Pollination mechanisms, reproduction and plant breeding (Vol. 2). ­ Springer Science & Business Media, Berlin, Germany, pp. 234. GALUN E., IZHAR S., ATSMON D., 1965 ­ Determination of relative auxin content in hermaphroditic and andrò monoecious Cucumis sativus L. ‐ Plant Physiol., 40(2): 321­326. Maniriho ‐ Dynamics of olive trees flowering and fruit set in Mediterranean basin 61 GREEN P.S., 2004 ­ Oleaceae, pp. 296­306. ­ In: KUBITZKI K., J.G. ROHWER, and V. BITTRICH (eds.) Flowering Plants.∙Dicotyledons. Springer, Berlin, Heidelberg, Germany, pp. 478. GUCCİ R., LODOLİNİ E.M., RAPOPORT H.F., 2007 ‐ Productivity of olive trees with different water status and crop load. ­ J. Hortic. Sci. Biotech., 82(4): 648­656. GUİTİAN P., 2006 ‐ Phenotypic gender variation within inflorescences of the protogynous species Helleborus foetidus L. (Ranunculaceae). ­ Plant System. Evol., 258(3­4): 137­146. HABERMAN A., DAG A., SHTERN N., ZİPORİ I., EREL R., BEN­ GAL A., YERMİYAHU U., 2019 ­ Significance of proper nitrogen fertilization for olive productivity in intensive cultivation. ­ Sci. Hortic., 246: 710­717. HACKETT W.P., HARTMANN H.T., 1967 ­ The influence of temperature on floral initiation in the olive. ­ Physiologia Plantarum, 20(2): 430­436. HAMANISHI E.T., CAMPBELL M.M., 2011 ­ Genome‐wide responses to drought in forest trees. ­ Forestry, 84: 273­ 283. HERRERA­CÁCERES C., PÉREZ­GALARCE F., ÁLVAREZ­ MİRANDA E., CANDİA­VÉJAR A., 2017 ­ Optimization of the harvest planning in the olive oil production: A case study in Chile. ­ Computers Electronics Agric., 141: 147­ 159. HUANG S.Q., 2003 ­ Flower dimorphism and the mainte‐ nance of andromonoecy in Sagittaria guyanensis ssp. Lappula (Alismataceae). ­ New Phytologist, 157(2): 357­ 364. Jİ X., SHİRAN B., WAN J., LEWİS D.C., JENKİNS C.L.D., CON­ DON A.G., RICHARDS R.A., DOLFERUS R., 2010 ‐ Importance of pre‐anthesis anther sink strength for maintenance of grain number during reproductive stage water stress in wheat. ­ Plant, Cell & Environ., 33(6): 926­942. KARAPATZAK E.K., WAGSTAFFE A., HADLEY P., BATTEY N.H., 2012 ­ High‐temperature‐induced reductions in cropping in everbearing strawberries (Fragaria × ana­ nassa) are associated with reduced pollen performance. ­ Annals Appl. Biol., 161(3): 255­265. KOUR D., BAKSHİ P., WALİ V.K., SHARMA N., SHARMA A., IQBAL M., 2018 ­ Alternate bearing in olive. A Review. ­ Int. J. Curr. Microbiol. App. Sci., 7(9): 2281­2297. LAVEE S., 2007 ­ Biennial bearing in olive (Olea europaea L.). ­ Annales Ser. His. Nat., 17: 101­112. LAVEE S., AVİDAN N., 1993 ­ Protein content and composi‐ tion of leaves and shoot bark in relation to the alterna‐ te bearing of olive trees (Olea europaea L.). ­ Acta Horticulturae, 356: 143­147. Lİ D., SHENG Y., NİU H., Lİ Z., 2019 ­ Gene interactions regulating sex determination in cucurbits. ­ Frontiers Plant Sci., 10: 10. LİSTER R., O’MALLEY R.C., TONTİ­FİLİPPİNİ J., GREGORY B.D., BERRY C.C., MİLLAR A.H., ECKER J.R., 2008 ­ Highly integrated single‐base resolution maps of the epigenome in Arabidopsis. ­ Cell, 133(3): 523­536. LOUMOU A., GİOURGA C., 2003 ­ Olive groves: “The life and identity of the Mediterranean’’. ­ Agric. Human Values, 20(1): 87­95. MARONE E., FİORİNO P., 2010 ­ The fate of lateral buds in the Olive (Olea europaea L. ssp. europaea var. europa‐ ea): A First Report. ­ Adv. Hort. Sci., 24(1): 29­34. MERT C., BARUT E., İPEK A., 2013 ­ Quantitative seasonal changes in the leaf phenolic content related to the alternate‐bearing patterns of olive (Olea europaea L. cv. Gemlik). ­ J. Agr. Sci. Tech., 15: 995­1006. MONSELİSE S.P., GOLDSCHMİDT E.E., 1982 ­ Alternate bea‐ ring in fruit trees. ­ Horticultural Reviews, 4(1): 128­ 173. NEWTON C., LORRE C., SAUVAGE C., IVORRA S., TERRAL J.F., 2014 ­ On the origins and spread of Olea europaea L. (olive) domestication: evidence for shape variation of olive stones at Ugarit, Late Bronze Age, Syria: a window on the Mediterranean Basin and on the westward diffu‐ sion of olive varieties. ­ Vegetation History Archaeobotany, 23(5): 567­575. RALLO L., TORRENO P., VARGAS A., ALVARADO J., 1993 ­ Dormancy and alternate bearing in olive. ­ Acta Horticulturae, 356: 127­136. REALE L., SGROMO C., EDERLİ L., PASQUALİNİ S., ORLANDİ F., FORNACİARİ M., FERRANTI F., ROMANO B., 2009 ­ Morphological and cytological development and starch accumulation in hermaphrodite and staminate flowers of olive (Olea europaea L.). ­ Sexual Plant Reproduction, 22(3): 109­119. ROSATİ A., CAPORALİ S., PAOLETTİ A., FAMİANİ F., 2011 ­ Pistil abortion is related to ovary mass in olive (Olea europaea L.). ­ Sci. Hortic., 127(4): 515­519. RUGİNİ E., BALDONİ L., MULEO R., SEBASTİANİ L., 2016 ­ The olive tree genome. ‐ Springer Verlag, Berlin, Germany, pp. 3­319. SEDGLEY M., GRİFFİN A.R., 2013 ­ Sexual reproduction of tree crops. ­ Academic Press. eBook, London, UK, pp. 378. SEIFI E., GUERIN J., KAISER B., SEDGLEY M., 2015 ­ Flowering and fruit set in olive: a review. ­ J. Plant Physiol., 5(2): 1263­1272. SOLOMON B.P., 1985 ­ Environmentally influenced changes in sex expression in an andromonoecious plant. ­ Ecology, 66(4): 1321­1332. SONG Y., TİAN M., Cİ D., ZHANG D., 2015 ­ Methylation of microRNA genes regulates gene expression in bisexual flower development in andromonoecious poplar. ­ J. Exper. Botany, 66(7): 1891­1905. TERRAL J.F., ALONSO N., CAPDEVİLA R.B.I., CHATTİ N., FABRE L., FİORENTİNO G., MARINVAL P., JORDÁ G.P., PRADAT B., ROVIRA N., ALİBERT P., 2004 ­ Historical biogeography of olive domestication (Olea europaea L.) as revealed by geometrical morphometry applied to biological and archaeological material. ­ J. Biogeography, 31(1): 63­77. Adv. Hort. Sci., 2022 36(1): 53­62 62 VINING K.J., POMRANING K.R., WILHELM L.J., PRIEST H.D., PELLEGRINI M., MOCKLER T.C., FREITAG M., STRAUSS S., 2012 ­ Dynamic DNA cytosine methylation in the Populus trichocarpa genome: tissue‐level variation and relationship to gene expression. ­ BMC Genomics, 13: 27. WİENS D., CALVİN C.L., WİLSON C.A., DAVERN C.I., FRANK D., SEAVEY S.R., 1987 ­ Reproductive success, sponta‐ neous embryo abortion, and genetic load in flowering plants. ­ Oecologia, 71(4): 501­509. ZHANG J., 1993 ­ Apple tree system research. ­ PhD Thesis, Department of Agricultural Sciences, Lincoln University, Lincoln, Canterbury, New Zealand. ZHANG X., YAZAKİ J., SUNDARESAN A., COKUS S., CHAN S.W.L., CHEN H., HENDERSON I.R., SHİNN P., PELLEGRI­ NI M., JACOBSEN S.E., ECKER J.R., 2006 ­ Genome‐wide high‐resolution mapping and functional analysis of DNA methylation in Arabidopsis. ­ Cell, 126(6): 1189­ 1201. ZILBERMAN D., GEHRING M., TRAN R.K., BALLINGER T., HENIKOFF S., 2007 ­ Genome‐wide analysis of Arabidopsis thaliana DNA methylation uncovers inter‐ dependence between methylation and transcription. ­ Nature Genetics, 39: 61­69.