In ternationa l Scholars Journa ls African Journal of Food Science Research ISSN 2375-0723 Vol. 5 (6), pp. 095-200, June, 2017. Available online at www.internationalscholarsjournals.org © International Scholars Journals Author(s) retain the copyright of this article. Full Length Research Paper Effect of cassava varieties and roasting methods on the chemical composition and pasting properties of tapioca grits Adebowale, A.A.*, Sanni, L. O. and Onitilo, M.O. Department of Food Science and Technology, University of Agriculture, Abeokuta, Nigeria Accepted 10 February 2016 This study investigated the chemical composition and pasting properties of tapioca grits from different cassava varieties and roasting methods. Tapioca grits (partially gelatinized irregular flakes from roasted cassava starch), were produced from three different cassava varieties (Odongbo, TMS 30572 and Oko-iyawo) and two roasting methods (traditional and rotary dryer). The chemical composition and pasting properties of the tapioca grits were evaluated. The results showed that the effect of cassava variety and roasting methods on chemical composition of tapioca grits was significantly different (P<0.05). The principal component analysis (PCA) of variation in the chemical properties of the tapioca grits indicated that moisture, sugar and starch accounted for 83% of the variation in the chemical properties of tapioca grits. This study showed that peak and hot paste viscosities are the key pasting parameters in characterizing tapioca grits from the cassava varieties and roasting methods studied and that variation in peak viscosity of the tapioca grits might be due more too varietal influence than the roasting method. Key words: Cassava, variety, roasting, tapioca grits, chemical, pasting. INTRODUCTION Cassava is one of the most drought tolerant crops and can be successfully grown on marginal soils, giving reasonable yields where many other crops do not grow well. The Collaborative Study on Cassava in Africa (COSCA) revealed that between 1961 and 1999, total cassava production in Africa nearly tripled from 33 million tonnes per year from 1961 to 1965, and to 87 million tones per year from 1995 to 1999, in contrast to the more moderate increases in Asia and Latin America (Nweke et al., 2002). Most of the increases in cassava production in Africa were achieved in Ghana and Nigeria. One of the advantages cassava has over other starchy crops is the variety of uses to which the roots can be put. Apart from being a staple food for humans (especially in Africa), it additionally has an excellent potential as livestock feed, and in textile, plywood, paper, brewing, chemical and pharmaceutical industries. Originally, the research focused on improved yields, cultivation practices and *Correspondence author. E-mail: deboraz2002@yahoo.com. crop protection. Cassava research has, since 1985, also focused on processing, quality control and new product development (Dufour et al., 2002). A major constraint to cassava utilization is that cassava deteriorates rapidly. Cassava has a shelf life of 24 – 48 h after harvest (Wenham, 1995). Hence, fresh cassava roots must be processed into a more shelf -stable form within 2 to 3 days from harvest. One of such cassava product is tapioca grit. Tapioca grit is a partially gelatinized dried cassava starch, which appears as flakes or irregularly shaped granules. It is consumed in many parts of West Africa, and widely accepted as a convenient diet (Hollesman and Ates, 1956). A number of reports are available in the literature on tapioca (Oyewole and Obieze, 1995; Sanni et al., 1997; Oyewole et al., 2003; Adebowale et al., 2006, 2007). There is no published work on the effect of cassava variety and roasting methods on chemical and pasting of tapioca grits. The main objective of this study therefore was to evaluate the effect of cassava varieties and roast- ing methods on the chemical composition and pasting properties of tapioca grits. Adebowale et al. 096 Table 1. Chemical components (%) of tapioca grits from different cassava variety and roasting method. Tapioca samples Moisture Sugar Starch Carbohydrate Amylose Protein Fat Odongbo Traditionally roasted 9.30 b 2.25 b 92.08 bc 94.34 b 23.12 a 0.24 a 0.25 c Odongbo rotary dryer roasted 10.53 b 2.59 c 94.75 d 97.34 c 22.95 a 0.23 a 0.21 bc TMS 30572 traditionally roasted 7.80 a 2.75 c 87.59 a 90.34 a 24.20 b 0.23 a 0.20 bc TMS 30572 rotary dryer roasted 7.20 a 2.09 ab 93.81 cd 95.90 bc 24.30 b 0.26 b 0.20 bc Oko-iyawo traditionally roasted 9.53 b 2.82 c 86.96 a 89.79 a 23.19 a 0.24 a 0.15 ab Oko-iyawo rotary dryer roasted 7.40 a 1.88 a 89.96 b 91.85 a 23.27 a 0.23 a 0.12 a Range 7.20-10.50 1.88-2.75 86.96-94.75 89.79-97.34 22.95-24.30 0.23-0.26 0.12-0.25 SD 1.35 0.38 3.32 3.08 0.59 0.01 0.05 CV 7.4 5.94 1.51 1.43 1.01 3.64 18.85 P of Variety *** ns *** *** *** ns *** P of Roasting Method ns *** *** *** ns ns ns P of Variety X Roasting Methods *** *** Ns ns ns *** ns *** Interactions significant at P<0.01 ns. Interactions not significantly different at P>0.05 Mean values having the same superscript with column are significantly different (P<0.05) MATERIALS AND METHODS Three cassava varieties (one improved variety; TMS30572, and two traditional varieties; Odongbo and Oko-iyawo) were obtained from the Teaching and Research Farm of the University of Agriculture, Abeokuta, Nigeria. The varieties were grown under rain-fed con- ditions. No fertilizer or herbicides were used, and hand weeding was done when necessary. All the cassava varieties were har- vested at 12 months after planting. The harvested roots were pro- cessed within 60 min after harvesting. Preparation of tapioca grits Fifty kg of freshly harvested cassava roots were peeled, washed with water and grated with a diesel engine powered mechanical grater (action zone made of 3 mm stainless steel, Nigerian made). The resultant pulp was immediately sieved through a screen and suspended in 70l of water. This separates the fibrous and other coarse root material from the starch pulp. The starch pulp was allowed to settle for 4 - 6 h before decanting. The thick starch cake at the bottom of the bowl was pressed to remove water. This was screened through a screen (20mesh/inch size) to produce coarse- grained moist starch flour. In the traditional method, the moist starch flour was roasted over an open firewood flame in a large, shallow stainless steel pan for 20 min at temperature range 120 - 150°C with constant stirring using a piece of stainless steel plate. Vegetable oil was used to rub the pan before roasting to prevent stickiness and burning (Adebowale et al., 2006). In the rotary dryer method, the moist starch flour was roast- ed in a locally fabricated electrically powered rotary dryer. Charcoal was used as heating source (Figure 1) . Roasting temperature was 100 o C and an elapsed time of 20 min. The dryer has a suction fan that removes steam generated during roasting. The fan was switched off during the first 10 min after loading the flour into the dryer to effect partial gelatinization. The fan was switched on thereafter to effect drying of the partially gelatinized starch granules (Sanni et al., 2006). The rotary dyer has a drying capacity of 250 kg/man day. Analytical determinations The moisture, protein and fat contents of the samples were deter- mined using the AOAC (1990) method. The starch and total sugars content were determined using a colorimetric method (Dubois et al., 1956). Absorbance was read at 490 nm using a spectrophotometer (model Spectronic 601, Milton Roy Company, USA). The amylase content was determined using the method of Williams et al (1958) involving the preparation of stock iodine solution and iodine rea- gent. Absorbance was read using a pectrophotometer at 620 nm. A blank was used to standardize the spectrophotometer. Pasting properties were determined using a Rapid Visco Analyser (RVA) (model RVA 3D+; Network Scientific, 5Australia). The sample was turned into slurry by mixing 3 g with 25 ml of water inside the RVA can and inserted into the tower, which was then lowered into the system. The slurry was heated from 50 to 95°C and cools back to 50°C within 12 min, rotating the can at a speed of 160 rpm with continuous stirring of the content with a plastic paddle. Parameters estimated were peak viscosity, setback viscosity, final viscosity, pasting temperature and time to reach peak viscosity. Statistical analysis Each analytical determination was carried out in three replicates. Data were subjected to analysis of variance (ANOVA) and principal component analysis (PCA) using SAS version 8e software (SAS Institute Inc., Cary, NC, USA) at P<0.05. Means were separated using Duncan’s Multiple Range Test. RESULTS AND DISCUSSIONS Chemical composition of tapioca grits from different cassava varieties and roasting methods The effect of cassava varieties and roasting methods on the chemical properties of tapioca grits from different cassava varieties and roasting methods are presented in Table 1. Rotary dryer roasted TMS 30572 tapioca grits had the least moisture while traditionally roasted tapioca grits from Odongbo cassava variety had the highest moisture content. The lower the initial moisture content of a product to be stored the better the storage stability of Table 2. Percentage variance explained by each chemical component of tapioca grits. Component Number Component Name Eigenvalues % of variance Cumulative % 1 Moisture 2.57 36.72 36.72 2 Sugar 2.11 30.11 66.83 3 Starch 1.16 16.53 83.36 4 Carbohydrate 0.68 9.68 93.05 5 Amylose 0.33 4.68 97.73 6 Protein 0.16 2.27 100.00 7 Fat 1.35E-07 1.93E-06 100.00 Extraction Method: Principal Component Analysis. the product. Also, the lower the initial moisture content of the product the more the efficiency of the drying method because this shows that much of the water contained in the fresh sample or product had been removed during drying (Pierre, 1989). The moisture content of all the tapioca grits were below the 10% stipulated standard of the revised regulation of the Standard Organization of Nigeria (SON, 1988; Sanni et al., 2005). All the tapioca grits can be stored for up to 7 months because their moisture contents were below the levels reported by Ukpabi and Ndimele (1990) who found that gari samples with a moisture content of < 16% but > 13% could be stored for 2- 7 months without mould infestation. The varietal effect on the moisture content of tapioca grits was significantly different (P<0.01), but the effect of roasting methods was not significantly different (P>0.05) . This indicates that irrespective of either traditional or rotary dryer roasting, tapioca grits with low moisture and hence longer shelf life can be produced from the cassava varieties processed. Tapioca grits from TMS 30572 traditionally roasted had the highest sugar content while rotary dryer roasted tapioca grits from Oko-iyawo cassava contained the minimum level of sugar. Traditionally roasted tapioca grits from Oko-iyawo cassava variety had the least content of starch while the rotary dryer roasted Odongbo variety tapioca grits had the highest starch content. Similar trend was displayed by the carbohydrate content. Tapioca grit is essentially a starchy product from moistened roasted cassava starch. The starch and sugar content for all tapioca grits were higher than values recorded by Oyewole and Obieze (1995). The amylose content of the tapioca grits ranged between 22.95% and 24.30%, with rotary dryer roasted tapioca grits recording the highest amylose content and rotary dryer roasted Odongbo tapioca grits containing the highest amount of amylose. The protein and fat content of the tapioca grits ranged from 0.23 to 0.26% and from 0.12 to 0.25% respectively. The amylose content of the tapioca grits were higher than that obtained by Oyewole and Obieze (1995). Raja and Ramakrishna (1990) reported that heat treatment causes a reduction in the amylose content of cassava, but this study showed little variation in the amylose content of tapioca grits from the two roasting methods. Also, the interactions between cassava variety and roasting me- thods on amylose content of the tapioca grits were not significantly different (P>0.05), but the varietal effect was significantly different (P<0.01). The parameters of Principal Component Analysis (PCA) are as shown in Table 2. The results showed that moisture, starch and sugar content accounted for 83% of the variations in the chemical properties of the tapioca samples. Hence, in characterizing the chemical com- position of tapioca grits from different cassava variety and roasting methods, moisture, sugar and starch are the key components of variance assessment. Pasting properties of tapioca grits from different cassava varieties and roasting methods The pasting profile of tapioca grits from different cassava varieties and roasting methods are shown in Table 3. Tapioca grits are usually cooked into paste referred to as tapioca meal before consumption, although some con- sumer’s prefer eating tapioca grits as snack. The pasting properties become important in predicting the behaviour of tapioca paste during and after cooking. When starch or starch-based foods are heated in water beyond a critical temperature, the granules absorb a large amount of water and swell to many times their original size. Over a critical temperature, which is characteristics of a parti-cular starch, the starch undergoes an irreversible process known as gelatinization. When the temperature rises above the gelatinization temperature, the starch granules begin to swell and viscosity increases on shearing. The temperature at the onset of this rise in viscosity is refer- red to as the pasting temperature. The pasting tempera- ture of the tapioca samples was lower than values report- ed for other cassava products (Sanni and Jaji, 2003; Sanni et al., 2004; Adebowale et al., 2005). The lower pasting temperature exhibited by tapioca grits is as a results of the partial gelatinization of starch granules during roasting. The pasting temperature is a measure of the minimum temperature required to cook a given food sample, it can have implications for the stability of other 097 Afr. J. Food Sci. Res. Adebowale et al. 098 Table 3. Pasting properties of tapioca grits from different cassava varieties and roasting methods. Tapioca Peak Trough Breakdown Final Set back Peak Pasting samples (RVU) (RVU) (RVU) Viscosity (RVU) viscosity (RVU) time (min) temperature (°C) Odongbo 449.30 b 162.55 a 285.91 b 210.89 a 48.33 a 3.85 bc 63.53 b Traditionally roasted 352.86 a 179.03 c 173.84 a 244.16 b 65.14 b 4.27 d 63.60 b Odongbo rotary dryer roasted 477.83 bc 176.78 bc 282.44 b 233.89 b 57.11 ab 3.76 b TMS 30572 63.07a traditionally roasted 476.52 bc 165.42 b 282.25 b 228.05 ab 62.64 b 3.63 a 63.17 a TMS 30572 rotary dryer roasted 513.55 c 178.44 c 325.19 c 237.14 b 58.70 ab 3.82 bc 63.17 a Oko-iyawo traditionally roasted 467.30 bc 167.97 ab 294.17 bc 226.81 ab 58.83 ab 3.89 c 63.17 a Oko-iyawo rotary dryer roasted Range 352.86- 162.55- 173.84- 210.89-244.16 48.33-65.14 3.63-4.27 63.07-63.60 513.55 179.03 325.19 6.48 2.63 10.28 4.59 11.56 1.13 0.21 P of Variety *** ns *** ns ns *** ** P of Roasting ** ns ** ns ** *** ns Method P of Variety X Ns *** ** ** ns *** ns Roasting Methods *** Interactions significant at P<0.01 **Interactions significant at P<0.05 ns Interactions not significantly different at P>0.05 components in a formula and also indicate energy costs (Newport Scientific, 1998). The pasting temperature of the tapioca grits is generally lower than the boiling temperature; hence the tapioca grits can form a paste in hot water below boiling point. This, at commercial level, is a remarkable cost saving. As the temperature increases during the pasting test, the granules rupture and amylose leaches into the solution, followed at a slower rate in some cases by the amylopectin fraction. The series of processes that follow gelatinization is collectively referred to as the pasting profile. Peak viscosity, which is the maximum viscosity, deve- loped during or soon after the heating portion of the past- ing test (Newport Scientific, 1998), is also lower com- pared to values obtained for dried fufu by Adebowale et al. (2005). This is also because tapioca is partially gelati- nized. Varietal differences exist in the peak viscosity of the tapioca grits at P<0.01, while the effect of different roasting methods is significant at P<0.05.The statistical significant (P>0.05). Therefore, variation in peak viscosity of the tapioca grits might be due more to genetic variation interaction between variety and roasting method is not than processing variable. Peak viscosity is often corre- lated with the final product quality. It also provides an indication of the viscous load likely to be encountered during mixing (Maziya- Dixon et al., 2004, 2005). It is customary to measure the peak time that occurs with peak viscosity. The peak time is a measure of the cooking time (Adebowale et al., 2005). The time to attain peak viscosity obtained for tapioca grits is considerably lower than that reported for other cassava product by Sanni et al. (2004) and Adebowale et al. (2005). This might be due to the fact that tapioca is partially gelati- nized. During the hold period of a typical pasting test, the sample is subjected to a period of constant temperature (usually 95°C) and mechanical shear stress. This further disrupts the starch granule and amylose molecules generally leach out into solution and align in the direction of the shear (Maziya-Dixon et al., 2005). The period is sometimes called shear thinning, holding strength, hot paste viscosity or trough due to the accompanied break- down in viscosity. It is the minimum viscosity value in the constant temperature phase of the RVA profile and mea- sures the ability of paste to withstand breakdown during 099 Afr. J. Food Sci. Res. Table 4. Percentage variance explained by each of the pasting parameters. Component number Component name Eigenvalues % of Variance Cumulative % 1 Peak 4.09 58 58 2 Trough 1.81 26 84 3 Breakdown 0.54 8 91 4 Final viscosity 0.43 6 98 5 Set back 0.12 2 99 6 Peak time 8.93E-03 0.13 100 7 Pasting temperature 1.91E-08 2.72E-07 100 Extraction Method: Principal Component Analysis. cooling (Newport Scientific, 1998). The effect of cassava variety as well as the roasting method on the hot paste viscosity of tapioca grits is not significant (P>0.05). But the interaction between variety and roasting method is significant at P<0.01. Large values indicate little break- down of sample starches. The rate of starch breakdown depends on the nature of the material, the temperature, and the degree of mixing and shear applied to the mixture (Newport Scientific, 1998) . The ability of a mixture to withstand heating and shear stress that is usually encountered during process- ing is an important factor for many processes especially those requiring stable paste and low retrogradetion/se- neresis. The breakdown viscosity recorded by traditio- nally roasted tapioca grits is higher than those of the rotary dryer method for all the three varieties. There are significant differences in the breakdown viscosity for tapioca grit from different cassava varieties and roasting methods (P<0.05). The final viscosity indicates the ability of the starch- based food to form a viscous paste or gel after cooking and cooling. The viscosity after cooling to 50°C repre- sents the setback or viscosity of cooked paste. It is a stage where retrogradation or re-ordering of starch mole- cules occurs. Sanni et al., (2004) stated that lower set- back during the cooling of the paste indicates greater resistance to retrogradation. Principal component analysis (PCA) for the pasting parameters of the tapioca grits showed that peak viscosity and hot paste viscosity accounted for 84% of the variance in the pasting profile of tapioca grits from different cassava variety and roasting methods (Table 4). Conclusion This study has revealed that in characterizing the che- mical composition of tapioca grits from different cassava variety and roasting methods, moisture, sugar and starch are the key component of variation in chemical composi- tion of tapioca. The pasting temperature of the tapioca grits is generally lower than the boiling temperature; hence the tapioca grits can form a paste in hot water below boiling point. This, at commercial level, is a remar- kable cost saving. This study found out peak and hot paste viscosities as the key pasting parameters in cha- racterizing tapioca grits from the cassava varieties and roasting methods studied and that variation in peak viscosity of the tapioca grits might be due more to genetic variation than roasting method. ACKNOWLEDGEMENT The authors wish to acknowledge the financial support by EU/INCDEV/UNAAB Cassava SME project and the Inte- grated Cassava Project, International Institute of Tropical agriculture, Ibadan, Nigeria. REFERENCES Adebowale AA, Sanni LO, Awonorin SO (2005). Effect of texture modifiers on the physicochemical and sensory properties of dried fufu. Food Sci. Technol. Inter. 11(5): 373-382. Adebowale AA, Sanni LO, Kuye A (2006). Effect of roasting methods on sorption isotherm of tapioca grits. Electronic J. Environ. Agric. Food Chem. 5 (6): 1649-1653 Adebowale AA, Sanni LO, Awonorin SO, Daniel IO, Kuye A(2007). Effect of cassava varieties on the sorption isotherm of tapioca grits. Inter. J. Food Sci. Technol. 42 (4): 448-452. AOAC. (1990). Official Methods of Analysis of the Association of Analytical Chemists. 15 th ed. Arlington, U.S.A. Dubios M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956). Colorimetric method for determination of sugars and related substances. Analy. 28: 350-356. Dufour D, O’Brein GM, Best R (2002). Cassava flour and starch: progress in research and development. International Center for Tropical Agriculture (CIAT), Apartado Aereo 6713, Cali, Colombia. Hollesman LWJ, Ates A (1956). Processing of cassava and cassava products in rural industry. FAO Agricultural Development paper. Maziya-Dixon B, Dixon AGO, Adebowale AA (2004). Targeting different end uses of cassava: genotypic variations for cyanogenic potentials and pasting properties. A paper presented at ISTRC-AB Symposium, 31 October – 5 November 2004, Whitesands Hotel, Mombassa, Kenya. Maziya-Dixon B, Sanni LO, Adebowale AA, Onabanjo OO, Dixon AGO (2005). Effect of variety and drying methods on proximate composition and pasting properties of high quality cassava flour from yellow cassava roots. In proceedings of the African Crop Science Society Conference, 5 th – 9 th December, 2005, Enttebe, Uganda. Newport Scientific (1998). Applications manual for the Rapid visco TM analyzer using thermocline for windows. Newport Scientific Pty Ltd., 1/2 Apollo Street, Warriewood NSW 2102, Australia. pp. 2-26. Adebowale et al. 200 Nweke FI, Dunstan S, John L (2002). The Cassava Transformation: Africa's Best Kept Secret. Michigan State University Press, East Lansing. p. 10. Oyewole OB, Obieze N (1995). Processing and Characteristics of "Tapioca" meal from cassava. Trop.l Sci. 35: 19-22. Oyewole OB, Sanni LO, Dipeolu OA, Adebayo K, Ayinde IA (2003). Development of the small and medium scale enterprise sector producing cassava based products to meet emerging urban demand in West Africa. Annual Report submitted toEU/NRI, December 2003 Pierre S (1989). Cassava. Macmillan Education Ltd. London. In cooporation with the Technical Centre for Agriculture and Rural Cooperation Wageningen, The Netherlands. pp. 1-2, pp. 30- 76. Raja KCM, Ramakrishma SV (1990). Compositional and pasting characteristics of plain-dried and parboiled cassava (Manihot esculenta, Crantz). Food Chem, 38: 79-88. Sanni LO, Adebowale AA, Filani TA, Oyewole OB, Westby A (2006). Quality of flash and rotary dried fufu flour. J. Food Agric. Environ. 4(3and4): 74-78. Sanni LO, Maziya-Dixo B, Akanya CI, Alaya Y, Egwuonwu CV, Okechukwu RU, Ezedinma C, Akoroda M, Lemchi J, Ogbe F, Okoro E, Tarawali G, Mkumbira J, Patino M, Ssemakula G, Dixon A(2005). Standards for cassava products and guidelines for export. International Institute of Tropical Agriculture, Ibadan, Nigeria. pp. 11- 39. Sanni LO, Atere C, Kuye A (1997). Moisture sorption isotherms of fufu and tapioca at different temperatures. J. Food Eng. 34(2), 203 - 212 Sanni LO, Jaji FF (2003). Effect of drying and roasting on the quality attributes of fufu powder. Inter. J. Food Properties. 6(2): 229-238. Sanni LO, Kosoko SB, Adebowale AA, Adeoye RJ (2004). The influence of palm oil and chemical modification on the pasting and sensory properties of fufu flour. Inter. J. Food properties 7(2): 229– 237. Standard Organization of Nigeria (SON) (1988). Nigeria industrial standard for gari. 188: 189 Ukpabi UJ, Ndimele C (1990). Evaluation of the quality of gari produced in Imo State, Nigeria, Niger. Food J. 8: 105-110. Wenham JE (1995). Postharvest deterioration of cassava, a biotechnology perspective. FAO Plant production and Protection FAO, Rome. Paper 130. Williams VR, Wu W, Tsai HY, Bates HG (1958). Varietal differences in amylose content of rice starch. J. Agric.Food Chem. 6: 47-48.