AJLM 10(1)_2021_Contents.indd


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Table of Contents

Original Research
An oral history of medical laboratory development in francophone 
West African countries
Winny Koster, Albert G. Ndione, Mourfou Adama, Ibrehima Guindo, 
Iyane Sow, Souleymane Diallo, Jean Sakandé, Pascale Ondoa
African Journal of Laboratory Medicine | Vol 10, No 1 | a1157 | 16 March 2021

Original Research
Multicentre study of the burden of multidrug-resistant bacteria in 
the aetiology of infected diabetic foot ulcers
Adeyemi T. Adeyemo, Babatope Kolawole, Vincent O. Rotimi, Aaron O. 
Aboderin
African Journal of Laboratory Medicine | Vol 10, No 1 | a1261 | 23 March 2021

Original Research
The growth patterns of the medical technology profession in  
South Africa
Malcolm T. Ellapen, Terry J. Ellapen, Yvonne Paul
African Journal of Laboratory Medicine | Vol 10, No 1 | a1164 | 23 April 2021

Original Research
Effects of TNF-α and IL-10-819 T>C single nucleotide polymorphisms 
on urogenital schistosomiasis in preschool children in Zimbabwe
Amos Marume, Theresa Chimponda, Arthur Vengesai, Caroline Mushayi, 
Jaclyn Mann, Takafira Mduluza
African Journal of Laboratory Medicine | Vol 10, No 1 | a1138 | 29 April 2021

Original Research
In-depth investigation of turn-around time of full blood count tests 
requested from a clinical haematology outpatient department in 
Cape Town, South Africa
Leonard Mutema, Zivanai Chapanduka, Fungai Musaigwa, 
Nomusa Mashigo
African Journal of Laboratory Medicine | Vol 10, No 1 | a1318 | 29 April 2021

Original Research
Improving laboratory quality and capacity through leadership and 
management training: Lessons from Zambia 2016–2018
Felicity Gopolang, Fales Zulu-Mwamba, Davy Nsama, Annika Kruuner, 
Dailes Nsofwa, Ishmael Kasvosve, Royce Gomo, Tiny Motlhabane, 
Bhavna Chohan, Olusegun Soge, Daniel Osterhage, Nancy Campbell, 
Michael Noble, Ann Downer, Jean-Frederic Flandin, Anya Nartker, 
Catherine Koehn, Linda K. Nonde, Aaron Shibemba, Clement B. Ndongmo, 
Martin Steinau, Lucy A. Perrone
African Journal of Laboratory Medicine | Vol 10, No 1 | a1225 | 30 April 2021

Original Research
Molecular characterisation of NPM1 and FLT3-ITD mutations in a central 
South African adult de novo acute myeloid leukaemia cohort
Jean F. Kloppers, André de Kock, Johané Cronjé, Anne-Cecilia van Marle
African Journal of Laboratory Medicine | Vol 10, No 1 | a1363 | 30 June 2021

Original Research
Could ante-mortem computed tomography be useful in forensic 
pathology of traumatic intracranial haemorrhage?
Mmachuene I. Hlahla, Moshibudi J. Selatole
African Journal of Laboratory Medicine | Vol 10, No 1 | a1040 | 29 July 2021

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Editorial
Call for emergency action to limit global temperature increases, 
restore biodiversity, and protect health
Lukoye Atwoli, Abdullah H. Baqui, Thomas Benfield, Raffaella Bosurgi, 
Fiona Godlee, Stephen Hancocks, Richard Horton, Laurie Laybourn-
Langton, Carlos Augusto Monteiro, Ian Norman, Kirsten Patrick, Nigel 
Praities, Marcel G.M. Olde Rikkert, Eric J. Rubin, Peush Sahni, Richard 
Smith, Nicholas J. Talley, Sue Turale, Damián Vázquez
African Journal of Laboratory Medicine | Vol 10, No 1 | a1707 | 20 September 2021

Editorial
Towards a fiercely urgent expansion of laboratory medicine  
in Africa
Iruka N. Okeke
African Journal of Laboratory Medicine | Vol 10, No 1 | a1785 | 17 December 2021

Opinion Paper
Maintaining routine HIV and tuberculosis testing services in 
sub-Saharan African countries in the context of COVID-19: 
Lessons learnt and opportunities for improvement
Collins O. Odhiambo, Anafi Mataka, Marguerite Massinga Loembe, 
Pascale Ondoa
African Journal of Laboratory Medicine | Vol 10, No 1 | a1413 | 17 June 2021

Opinion Paper
Impact of COVID-19 on blood donation and supply in Africa
Kenneth B. David, Knovicks Simfukwe, Mohamed B. Musa, Steven 
Munharo, Don E. Lucero-Prisno III
African Journal of Laboratory Medicine | Vol 10, No 1 | a1408 | 25 October 2021

Original Research
Performance of International AIDS Vaccine Initiative African 
clinical research laboratories in standardised ELISpot and peripheral 
blood mononuclear cell processing in support of HIV vaccine  
clinical trials
Robert K. Langat, Bashir Farah, Jackton Indangasi, Simon Ogola, 
Gloria Omosa-Manyonyi, Omu Anzala, Jean Bizimana, Emmanuel 
Tekirya, Caroline Ngetsa, Moses Silwamba, Enoch Muyanja, 
Paramesh Chetty, Maureen Jangano, Nancy Hills, Jill Gilmour, 
Len Dally, Josephine H. Cox, Peter Hayes
African Journal of Laboratory Medicine | Vol 10, No 1 | a1056 | 17 February 2021

Original Research
Knowledge, attitude and practices of COVID-19 among medical 
laboratory professionals in Zambia
Adon Chawe, Ruth L. Mfune, Paul M. Syapiila, Sharon D. Zimba,  
Pipina A. Vlahakis, Samson Mwale, Kapambwe Mwape, 
Memory Chirambo-Kalolekesha, Misheck Chileshe, Joseph Mutale, 
Tobela Mudenda, Grace Manda, Victor Daka
African Journal of Laboratory Medicine | Vol 10, No 1 | a1403 | 04 March 2021

Original Research
Involvement of CD95 and ligand in CD4+ T-cell and CD8+ 
T-cell depletion and hepatic cytolysis in patients with 
chronic viral hepatitis B
Franklin S. Azebaze Agueguia, Paul Talla, Marie C. Okomo Assoumou, 
Graeme B. Jacobs, Cedric H. Mbakam, Elise Guiedem, Martha Tongo 
Mesembe, Emilia Lyonga, George Mondinde Ikomey
African Journal of Laboratory Medicine | Vol 10, No 1 | a1224 | 15 March 2021

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Vol 10, No 1 (2021)

ISSN: 2225-2002 (print)  |  ISSN: 2225-2010 (online)African Journal of Laboratory Medicine



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Table of Contents

Original Research
Comparison of 24-hour versus random urine samples for determination 
and quantification of Bence Jones protein in a South African population
Ashandree Reddy, Nadine Rapiti, Verena Gounden
African Journal of Laboratory Medicine | Vol 10, No 1 | a1228 | 04 August 2021

Original Research
Operational analysis of the national sickle cell screening programme  
in the Republic of Uganda
Arielle G. Hernandez, Charles Kiyaga, Thad A. Howard, Isaac Ssewanyana, 
Grace Ndeezi, Jane R. Aceng, Russell E. Ware
African Journal of Laboratory Medicine | Vol 10, No 1 | a1303 | 12 August 2021

Original Research
Higher proportion of non-classical and intermediate monocytes in  
newly diagnosed multiple myeloma patients in Egypt: A possible 
prognostic marker
Asmaa M. Zahran, Hanaa Nafady-Hego, Sawsan M. Moeen,  
Hanan A. Eltyb, Mohammed M. Wahman, Asmaa Nafady
African Journal of Laboratory Medicine | Vol 10, No 1 | a1296 | 25 August 2021

Original Research
Pre-evaluation assessment of serological-based COVID-19 point-of-care 
lateral flow assays in Kenya
James H. Kimotho, Abdiaziz A. Gosar, Ronald Inyangala, Paulyne Wairimu, 
Fred Siyoi, Damaris Matoke-Muhia, Cecilia Wanjala, Jeremiah Zablon, 
Moses Orina, Lucy Muita, Jacqueline Thiga, Lameck Nyabuti, Eunice 
Wainaina, Joseph Mwangi, Alice Mumbi, Samuel Omari, Ann Wanjiru, 
Samson M. Nzou, Missiani Ochwoto
African Journal of Laboratory Medicine | Vol 10, No 1 | a1317 | 17 September 2021

Original Research
Molecular red cell genotyping of rare blood donors in South Africa to 
enhance rare donor-patient blood matching
Lavendri Govender, Rosaley D. Prakashchandra, Pavitra Pillay, Ute Jentsch
African Journal of Laboratory Medicine | Vol 10, No 1 | a1400 | 27 September 2021

Original Research
Establishing the cost of Xpert MTB/RIF mobile testing in high-burden  
peri-mining communities in South Africa
Naseem Cassim, Lindi M. Coetzee, Abel L. Makuraj, Wendy S. Stevens, 
Deborah K. Glencross
African Journal of Laboratory Medicine | Vol 10, No 1 | a1229 | 30 November 2021

Original Research
Relationship between amino acid ratios and decline in estimated 
glomerular filtration rate in diabetic and non-diabetic patients in 
South Africa
Thapelo Mbhele, Donald M. Tanyanyiwa, Refilwe J. Moepya,  
Sindeep Bhana, Maya M. Makatini
African Journal of Laboratory Medicine | Vol 10, No 1 | a1398 | 10 December 2021

Original Research
Effect of polyethylene glycol 20 000 on protein extraction efficiency  
of formalin-fixed paraffin-embedded tissues in South Africa
Sophia Rossouw, Hocine Bendou, Liam Bell, Jonathan Rigby,  
Alan Christoffels
African Journal of Laboratory Medicine | Vol 10, No 1 | a1122 | 17 December 2021

Scientific Letter
Post-mortem diagnosis of COVID-19
Rujittika Mungmungpuntipantip, Viroj Wiwanitkit
African Journal of Laboratory Medicine | Vol 10, No 1 | a1471 | 24 March 2021

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Scientific Letter
Biobanking of COVID-19 specimens during the pandemic: The need  
for enhanced biosafety
Olayinka S. Ilesanmi, Aanuoluwapo A. Afolabi
African Journal of Laboratory Medicine | Vol 10, No 1 | a1379 | 21 October 2021

Lessons from the Field
From benchmarking to best practices: Lessons from the laboratory  
quality improvement programme at the military teaching hospital  
in Cotonou, Benin
Alban Zohoun, Tatiana B. Agbodandé, Angélique Kpadé, Raliatou O. Goga, 
René Gainsi, Paul Balè, Bibata M. Sambo, Remi Charlebois, Rachel Crane, 
Michele Merkel, Ludovic Anani, Ekaterina Milgotina
African Journal of Laboratory Medicine | Vol 10, No 1 | a1057 | 11 February 2021

Lessons from the Field
The deployment of mobile diagnostic laboratories for Ebola virus  
disease diagnostics in Sierra Leone and Guinea
Lance D. Presser, Jeanette Coffin, Lamine Koivogui, Allan Campbell,  
Julian Campbell, Fatmata Barrie, Jone Ngobeh, Zein Souma, Samuel Sorie, 
Doris Harding, Alimou Camara, Pepe Tohonamou, Basala Traore,  
Frank A. Hamill, Joe Bogan, Sharon Altmann, Casey Ross, Jay Mansheim, 
Robert Hegerty, Scott Poynter, Scott Shearrer, Carmen Asbun, Brendan 
Karlstrand, Phil Davis, Jane Alam, David Roberts, Paul D. Stamper,  
Jean Ndjomou, Nadia Wauquier, Mohamed Koroma, Alhaji Munu,  
Jason McClintock, Mar Mar, True Burns, Stephen Krcha
African Journal of Laboratory Medicine | Vol 10, No 1 | a1414 | 22 October 2021

Lessons from the Field
Setting up a molecular diagnostic laboratory for SARS-CoV-2 testing: 
Experience of a single centre in a resource-constrained setting
Iriagbonse I. Osaigbovo, Isaac O. Igbarumah, Ekene B. Muoebonam, 
Darlington E. Obaseki
African Journal of Laboratory Medicine | Vol 10, No 1 | a1326 | 30 March 2021

Lessons from the Field
Adaptation of an electronic dashboard to monitor HIV viral load  
testing in Côte d’Ivoire
Mary Kirk, Paul H. Assoa, Casey Iiams-Hauser, Yves-Rolland Kouabenan, 
Jennifer Antilla, Caleb Steele-Lane, Greg Rossum, Pascal Komena,  
Patricia Sadate Ngatchou, Nadine Abiola, Alain Kouakou,  
Adama Pongathie, Jean B. Koffi, Christiane Adje, Lucy A. Perrone
African Journal of Laboratory Medicine | Vol 10, No 1 | a1284 | 17 May 2021

Lessons from the Field
Strategic site selection for placement of HIV early infant diagnosis point-
of-care technology within a national diagnostic network in Lesotho
Anafi Mataka, Esther A.J. Tumbare, Tsietso Motsoane, David Holtzman, 
Monkoe Leqheka, Kolisang Phatsoane, Emma Sacks, Anthony Isavwa, 
Appolinaire Tiam
African Journal of Laboratory Medicine | Vol 10, No 1 | a1156 | 24 August 2021

Lessons from the Field
African countries established COVID-19 testing in one month: Here’s  
how they did it
Timothy Amukele, Ryland N. Spence
African Journal of Laboratory Medicine | Vol 10, No 1 | a1457 | 15 December 2021

Case Study
Emphysematous pyelonephritis in an infant from Sokoto,  
north-western Nigeria
Fatima B. Jiya, Paul K. Ibitoye, Nma M. Jiya, Maryam Amodu-Sanni, 
Yahaya Mohammed, Dada M. Aquib, Lukman K. Coker
African Journal of Laboratory Medicine | Vol 10, No 1 | a1181 | 26 April 2021

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Brief Report
Epstein-Barr virus, human papillomavirus and herpes simplex  
virus 2 co-presence severely dysregulates miRNA expression
Jude O. Okoye, Anthony A. Ngokere, Charles C. Onyenekwe,  
Olaposi Omotuyi, Deborah I. Dada
African Journal of Laboratory Medicine | Vol 10, No 1 | a975 | 16 March 2021

Brief Report
Performance of TaqMan probes for the detection of sexually  
transmitted infections in South African women
Nireshni Mitchev, Ravesh Singh, Nigel Garrett, Veron Ramsuran,  
Abraham J. Niehaus, Koleka P. Mlisana
African Journal of Laboratory Medicine | Vol 10, No 1 | a1124 | 31 March 2021

Brief Report
Cross-validation of a high-performance liquid chromatography nevirapine 
plasma assay in a resource-limited setting in Zimbabwe
Faithful Makita-Chingombe, Anthony T. Podany, Timothy Mykris,  
Farai Muzambi, Richard W. Browne, Andrew J. Ocque, Robin DiFrancesco, 
Lee C. Winchester, Courtney V. Fletcher, Tinashe Mudzviti,  
Charles C. Maponga, Gene D. Morse
African Journal of Laboratory Medicine | Vol 10, No 1 | a1264 | 08 July 2021

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Brief Report
Re-decontamination of liquid mycobacterial cultures: Additional 
Mycobacterium tuberculosis yield in the era of Xpert MTB/RIF  
Ultra in Cape Town, South Africa
Dawood da Costa, Pieter Nel
African Journal of Laboratory Medicine | Vol 10, No 1 | a1529 | 10 December 2021

Correction
Corrigendum: Brucellosis – Laboratory workers’ nightmare come true:  
A case study
Lebogang Skosana, Farzana Ismail, Nontombi Mbelle, Mohamed Said
African Journal of Laboratory Medicine | Vol 10, No 1 | a1690 | 22 November 2021

Reviewer Acknowledgement
African Journal of Laboratory Medicine | Vol 10, No 1 | a1812 | 22 December 2021

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