id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
ajlm-1122	Rossouw, Sophia; Bendou, Hocine; Bell, Liam; Rigby, Jonathan; Christoffels, Alan	Effect of polyethylene glycol 20 000 on protein extraction efficiency of formalin-fixed paraffin-embedded tissues in South Africa	2021		.htm	text/html	6301	370	43	This places limitations on the choice of proteomics workflows (including protein extraction, protein sample enrichment, fractionation and digestion) that can be used to generate samples of suitable quality for high sensitivity liquid chromatography (LC) tandem MS analysis.2,7 Additional challenges faced in FFPE proteomics studies, which cannot be remedied after the fact, are pre-analytical factors that affect protein extraction efficiency and often produce variable protein yields. In addition, protein samples extracted without PEG showed higher reproducibility, and the addition of PEG to the protein extraction buffer generated lower percentages of unique peptides remaining in the sample pellets.	cache/ajlm-1122.htm	txt/ajlm-1122.txt