id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
ajlm-2107	Mwakyabala, Jenipher G.; Mtemisika, Conjester I.; Mshana, Stacy; Mwakyoma, Adam A.; Silago, Vitus	Characterisation of genes encoding for extended spectrum β-lactamase in Gram-negative bacteria causing healthcare-associated infections in Mwanza, Tanzania	2023		.htm	text/html	2788	140	44	FIGURE 1: Molecular characterisation of extended spectrum β-lactamase genes by multiplex polymerase chain reaction assay, Bugando Medical Centre, Mwanza, Tanzania, January 2022 – July 2022. TABLE 2: Description of ceftriaxone-resistant GNB recovered for multiplex polymerase chain reaction amplification and detection of extended spectrum β-lactamase genes, Bugando Medical Centre, Mwanza, Tanzania, January 2022 – July 2022. TABLE 3: Results of disk combination method and multiplex PCR assay, and distributions of ESBL genes, Bugando Medical Centre, Mwanza, Tanzania, January 2022 – July 2022. Briefly, 2 µL of each DNA sample was added into a PCR reaction tube containing HotStarTaq DNA polymerase master mix (New England Biolabs; Hitchin, Hertfordshire, United Kingdom) and a set of primers (Table 1), resulting in a final PCR reaction volume of 25 µL. The thermal cycler (T100™, BIO-RAD, Kaki-Bukit, Singapore) was run with the following conditions: initial denaturation at 95 °C for 5 min; 30 cycles of denaturation at 94 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 1 min; and a final extension at 72 °C for 10 min. Products were detected by using a 1% agarose gel with Tris-acetate-EDTA buffer stained with SafeViewTM DNA stain (ABM; Richmond, British Colombia, Canada) and visualised under ultraviolet light. TABLE 1: Sequences of primers used for multiplex polymerase chain reaction assays for extended spectrum β-lactamase genes, Bugando Medical Centre, Mwanza, Tanzania, January 2022 – July 2022.	cache/ajlm-2107.htm	txt/ajlm-2107.txt