id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
ajlm-2252	Hurwitz, Jessica S.; Newton-Foot, Mae; Nel van Zyl, Kristien; Nel, Pieter	Fosfomycin susceptibility testing and resistance mechanisms in Enterobacterales in South Africa	2024		.htm	text/html	4228	218	37	For disk diffusion, EUCAST recommends disregarding isolated colonies that are within the ZOI, while CLSI advises taking them into account.17,18 For gradient diffusion, the manufacturer’s guidelines suggest different approaches for E. coli and K. pneumoniae: for E. coli, single colonies in the zone of the ellipse should be disregarded, while for K. pneumoniae, colonies within 3 mm of the strip should be considered for both CLSI and EUCAST interpretations.20 Fosfomycin resistance mechanisms Whole genome sequencing analysis Whole genome sequencing and genome assembly were previously performed on the E. coli and K. pneumoniae isolates using the Illumina MiSeq platform (Illumina, Inc., San Diego, California, United States).21 Resistance Gene Identifier version 5.2.1 (McMaster University, South Hamilton, Ontario, Canada) was used to identify fosfomycin resistance mechanisms in the assemblies, including fosA genes and the multidrug transporter mdtG, and mutations in the target gene murA, the uhpT and glpT transporter genes, and the cyaA and ptsI regulator genes, using the Comprehensive Antibiotic Resistance Database (McMaster University, South Hamilton, Ontario, Canada).22 Outputs were generated for 95% identity. Fosfomycin resistance mechanisms in 92 clinical isolates from South Africa (2017–2018). TABLE 3: Combinations of fosfomycin resistance mechanisms and minimum inhibitory concentration distributions for 92 clinical isolates from South Africa (2017–2018).	cache/ajlm-2252.htm	txt/ajlm-2252.txt