id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
ajlm-658	Senamela, Tshiphiri; Kock, Marleen; Becker, Piet; Potgieter, Joachim J.C.	Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay	2018		.htm	text/html	2714	147	51	The ratio of wild-type to mutant probe that provided optimal results was 1:8 (Figures 1 and 2 show how a heterozygous sample amplifies when the 1:1 and 1:8 ratios of mutant to wild-type probes were used). During the experiment, it was noted that the standard 1:1 wild-type to mutant probe ratio did not provide optimal results.	cache/ajlm-658.htm	txt/ajlm-658.txt