id	sid	tid	token	lemma	pos
ajlm-15	1	1	article	article	PROPN
ajlm-15	1	2	information	information	NOUN
ajlm-15	1	3	author	author	NOUN
ajlm-15	1	4	:	:	PUNCT
ajlm-15	1	5	corena	corena	PROPN
ajlm-15	1	6	de	de	PROPN
ajlm-15	1	7	beer1	beer1	PROPN
ajlm-15	1	8	monika	monika	PROPN
ajlm-15	1	9	esser2	esser2	PROPN
ajlm-15	1	10	wolfgang	wolfgang	PROPN
ajlm-15	1	11	preiser1	preiser1	PROPN
ajlm-15	1	12	affiliation	affiliation	NOUN
ajlm-15	1	13	:	:	PUNCT
ajlm-15	1	14	1department	1department	NUM
ajlm-15	1	15	of	of	ADP
ajlm-15	1	16	pathology	pathology	NOUN
ajlm-15	1	17	(	(	PUNCT
ajlm-15	1	18	division	division	NOUN
ajlm-15	1	19	of	of	ADP
ajlm-15	1	20	medical	medical	ADJ
ajlm-15	1	21	virology	virology	NOUN
ajlm-15	1	22	)	)	PUNCT
ajlm-15	1	23	,	,	PUNCT
ajlm-15	1	24	university	university	NOUN
ajlm-15	1	25	of	of	ADP
ajlm-15	1	26	stellenbosch	stellenbosch	PROPN
ajlm-15	1	27	,	,	PUNCT
ajlm-15	1	28	south	south	ADJ
ajlm-15	1	29	africa2department	africa2department	NOUN
ajlm-15	1	30	of	of	ADP
ajlm-15	1	31	pathology	pathology	NOUN
ajlm-15	1	32	(	(	PUNCT
ajlm-15	1	33	immunology	immunology	NOUN
ajlm-15	1	34	unit	unit	NOUN
ajlm-15	1	35	)	)	PUNCT
ajlm-15	1	36	,	,	PUNCT
ajlm-15	1	37	university	university	NOUN
ajlm-15	1	38	of	of	ADP
ajlm-15	1	39	stellenbosch	stellenbosch	PROPN
ajlm-15	1	40	,	,	PUNCT
ajlm-15	1	41	south	south	PROPN
ajlm-15	1	42	africa	africa	PROPN
ajlm-15	1	43	correspondence	correspondence	NOUN
ajlm-15	1	44	to	to	ADP
ajlm-15	1	45	:	:	PUNCT
ajlm-15	1	46	corena	corena	PROPN
ajlm-15	1	47	de	de	X
ajlm-15	1	48	beer	beer	PROPN
ajlm-15	1	49	postal	postal	ADJ
ajlm-15	1	50	address	address	NOUN
ajlm-15	1	51	:	:	PUNCT
ajlm-15	1	52	department	department	NOUN
ajlm-15	1	53	of	of	ADP
ajlm-15	1	54	pathology	pathology	PROPN
ajlm-15	1	55	,	,	PUNCT
ajlm-15	1	56	university	university	PROPN
ajlm-15	1	57	of	of	ADP
ajlm-15	1	58	stellenbosch	stellenbosch	PROPN
ajlm-15	1	59	,	,	PUNCT
ajlm-15	1	60	po	po	PROPN
ajlm-15	1	61	box	box	PROPN
ajlm-15	1	62	19063	19063	NUM
ajlm-15	1	63	,	,	PUNCT
ajlm-15	1	64	tygerberg	tygerberg	PROPN
ajlm-15	1	65	7505	7505	NUM
ajlm-15	1	66	,	,	PUNCT
ajlm-15	1	67	south	south	PROPN
ajlm-15	1	68	africa	africa	PROPN
ajlm-15	1	69	dates	date	NOUN
ajlm-15	1	70	:	:	PUNCT
ajlm-15	1	71	received	receive	VERB
ajlm-15	1	72	:	:	PUNCT
ajlm-15	1	73	09	09	NUM
ajlm-15	1	74	dec	dec	PROPN
ajlm-15	1	75	.	.	PROPN
ajlm-15	1	76	2012	2012	NUM
ajlm-15	1	77	accepted	accept	VERB
ajlm-15	1	78	:	:	PUNCT
ajlm-15	1	79	15	15	NUM
ajlm-15	1	80	aug	aug	PROPN
ajlm-15	1	81	.	.	PROPN
ajlm-15	1	82	2012	2012	NUM
ajlm-15	1	83	published	publish	VERB
ajlm-15	1	84	:	:	PUNCT
ajlm-15	1	85	15	15	NUM
ajlm-15	1	86	oct	oct	NOUN
ajlm-15	1	87	.	.	PROPN
ajlm-15	1	88	2012	2012	NUM
ajlm-15	1	89	how	how	SCONJ
ajlm-15	1	90	to	to	PART
ajlm-15	1	91	cite	cite	VERB
ajlm-15	1	92	this	this	DET
ajlm-15	1	93	article	article	NOUN
ajlm-15	1	94	:	:	PUNCT
ajlm-15	1	95	de	de	X
ajlm-15	1	96	beer	beer	PROPN
ajlm-15	1	97	c	c	NOUN
ajlm-15	1	98	,	,	PUNCT
ajlm-15	1	99	esser	esser	PROPN
ajlm-15	1	100	m	m	PROPN
ajlm-15	1	101	,	,	PUNCT
ajlm-15	1	102	preiser	preiser	PROPN
ajlm-15	1	103	w.	w.	PROPN
ajlm-15	1	104	optimising	optimise	VERB
ajlm-15	1	105	automation	automation	NOUN
ajlm-15	1	106	of	of	ADP
ajlm-15	1	107	a	a	DET
ajlm-15	1	108	manual	manual	ADJ
ajlm-15	1	109	enzyme	enzyme	NOUN
ajlm-15	1	110	-	-	PUNCT
ajlm-15	1	111	linked	link	VERB
ajlm-15	1	112	immunosorbent	immunosorbent	NOUN
ajlm-15	1	113	assay	assay	PROPN
ajlm-15	1	114	.	.	PUNCT
ajlm-15	2	1	afr	afr	PROPN
ajlm-15	2	2	j	j	PROPN
ajlm-15	2	3	lab	lab	PROPN
ajlm-15	2	4	med	med	PROPN
ajlm-15	2	5	.	.	PUNCT
ajlm-15	3	1	2011;1(1	2011;1(1	NUM
ajlm-15	3	2	)	)	PUNCT
ajlm-15	3	3	,	,	PUNCT
ajlm-15	3	4	art	art	NOUN
ajlm-15	3	5	.	.	PUNCT
ajlm-15	4	1	#	#	SYM
ajlm-15	4	2	15	15	NUM
ajlm-15	4	3	,	,	PUNCT
ajlm-15	4	4	3	3	NUM
ajlm-15	4	5	pages	page	NOUN
ajlm-15	4	6	.	.	PUNCT
ajlm-15	5	1	http://dx.doi.org/10.4102/	http://dx.doi.org/10.4102/	NUM
ajlm-15	5	2	ajlm.v1i1.15	ajlm.v1i1.15	PROPN
ajlm-15	5	3	copyright	copyright	NOUN
ajlm-15	5	4	notice	notice	NOUN
ajlm-15	5	5	:	:	PUNCT
ajlm-15	5	6	©	©	PROPN
ajlm-15	5	7	2012	2012	NUM
ajlm-15	5	8	.	.	PUNCT
ajlm-15	6	1	the	the	DET
ajlm-15	6	2	authors	author	NOUN
ajlm-15	6	3	.	.	PUNCT
ajlm-15	7	1	licensee	licensee	NOUN
ajlm-15	7	2	:	:	PUNCT
ajlm-15	7	3	aosis	aosis	NOUN
ajlm-15	7	4	openjournals	openjournal	NOUN
ajlm-15	7	5	.	.	PUNCT
ajlm-15	8	1	this	this	PRON
ajlm-15	8	2	is	be	AUX
ajlm-15	8	3	an	an	DET
ajlm-15	8	4	open	open	ADJ
ajlm-15	8	5	access	access	NOUN
ajlm-15	8	6	article	article	NOUN
ajlm-15	8	7	distributed	distribute	VERB
ajlm-15	8	8	under	under	ADP
ajlm-15	8	9	the	the	DET
ajlm-15	8	10	terms	term	NOUN
ajlm-15	8	11	of	of	ADP
ajlm-15	8	12	the	the	DET
ajlm-15	8	13	creative	creative	ADJ
ajlm-15	8	14	commons	common	NOUN
ajlm-15	8	15	attribution	attribution	NOUN
ajlm-15	8	16	license	license	NOUN
ajlm-15	8	17	,	,	PUNCT
ajlm-15	8	18	which	which	PRON
ajlm-15	8	19	permits	permit	VERB
ajlm-15	8	20	unrestricted	unrestricted	ADJ
ajlm-15	8	21	use	use	NOUN
ajlm-15	8	22	,	,	PUNCT
ajlm-15	8	23	distribution	distribution	NOUN
ajlm-15	8	24	,	,	PUNCT
ajlm-15	8	25	and	and	CCONJ
ajlm-15	8	26	reproduction	reproduction	NOUN
ajlm-15	8	27	in	in	ADP
ajlm-15	8	28	any	any	DET
ajlm-15	8	29	medium	medium	NOUN
ajlm-15	8	30	,	,	PUNCT
ajlm-15	8	31	provided	provide	VERB
ajlm-15	8	32	the	the	DET
ajlm-15	8	33	original	original	ADJ
ajlm-15	8	34	work	work	NOUN
ajlm-15	8	35	is	be	AUX
ajlm-15	8	36	properly	properly	ADV
ajlm-15	8	37	cited	cite	VERB
ajlm-15	8	38	.	.	PUNCT
ajlm-15	9	1	optimising	optimise	VERB
ajlm-15	9	2	automation	automation	NOUN
ajlm-15	9	3	of	of	ADP
ajlm-15	9	4	a	a	DET
ajlm-15	9	5	manual	manual	ADJ
ajlm-15	9	6	enzyme	enzyme	NOUN
ajlm-15	9	7	-	-	PUNCT
ajlm-15	9	8	linked	link	VERB
ajlm-15	9	9	immunosorbent	immunosorbent	NOUN
ajlm-15	9	10	assay	assay	NOUN
ajlm-15	9	11	in	in	ADP
ajlm-15	9	12	this	this	DET
ajlm-15	9	13	original	original	ADJ
ajlm-15	9	14	research	research	NOUN
ajlm-15	9	15	...	...	PUNCT
ajlm-15	9	16	open	open	ADJ
ajlm-15	9	17	access	access	NOUN
ajlm-15	9	18	•	•	ADP
ajlm-15	9	19	abstract	abstract	ADJ
ajlm-15	9	20	•	•	NUM
ajlm-15	9	21	introduction	introduction	NOUN
ajlm-15	9	22	•	•	NOUN
ajlm-15	9	23	materials	material	NOUN
ajlm-15	9	24	and	and	CCONJ
ajlm-15	9	25	methods	method	NOUN
ajlm-15	9	26	   	   	SPACE
ajlm-15	9	27	•	•	NUM
ajlm-15	9	28	automation	automation	NOUN
ajlm-15	9	29	of	of	ADP
ajlm-15	9	30	the	the	DET
ajlm-15	9	31	mk016	mk016	VERB
ajlm-15	9	32	enzyme	enzyme	NOUN
ajlm-15	9	33	-	-	PUNCT
ajlm-15	9	34	linked	link	VERB
ajlm-15	9	35	immunosorbent	immunosorbent	NOUN
ajlm-15	9	36	assay	assay	ADJ
ajlm-15	9	37	•	•	NUM
ajlm-15	9	38	results	result	VERB
ajlm-15	9	39	•	•	DET
ajlm-15	9	40	discussion	discussion	NOUN
ajlm-15	9	41	•	•	NOUN
ajlm-15	9	42	acknowledgements	acknowledgement	NOUN
ajlm-15	9	43	   	   	SPACE
ajlm-15	9	44	•	•	NOUN
ajlm-15	9	45	competing	compete	VERB
ajlm-15	9	46	interests	interest	NOUN
ajlm-15	9	47	   	   	SPACE
ajlm-15	9	48	•	•	NUM
ajlm-15	9	49	authors	author	NOUN
ajlm-15	9	50	’	'	PUNCT
ajlm-15	9	51	contributions	contribution	NOUN
ajlm-15	9	52	•	•	NUM
ajlm-15	9	53	references	reference	NOUN
ajlm-15	9	54	abstract	abstract	ADJ
ajlm-15	9	55	top	top	PROPN
ajlm-15	9	56	↑	↑	PROPN
ajlm-15	9	57	objective	objective	NOUN
ajlm-15	9	58	:	:	PUNCT
ajlm-15	9	59	enzyme	enzyme	NOUN
ajlm-15	9	60	-	-	PUNCT
ajlm-15	9	61	linked	link	VERB
ajlm-15	9	62	immunosorbent	immunosorbent	ADJ
ajlm-15	9	63	assays	assay	NOUN
ajlm-15	9	64	(	(	PUNCT
ajlm-15	9	65	elisas	elisas	PROPN
ajlm-15	9	66	)	)	PUNCT
ajlm-15	9	67	are	be	AUX
ajlm-15	9	68	widely	widely	ADV
ajlm-15	9	69	used	use	VERB
ajlm-15	9	70	to	to	PART
ajlm-15	9	71	quantify	quantify	VERB
ajlm-15	9	72	immunoglobulin	immunoglobulin	NOUN
ajlm-15	9	73	levels	level	NOUN
ajlm-15	9	74	induced	induce	VERB
ajlm-15	9	75	by	by	ADP
ajlm-15	9	76	infection	infection	NOUN
ajlm-15	9	77	or	or	CCONJ
ajlm-15	9	78	vaccination	vaccination	NOUN
ajlm-15	9	79	.	.	PUNCT
ajlm-15	10	1	compared	compare	VERB
ajlm-15	10	2	to	to	ADP
ajlm-15	10	3	conventional	conventional	ADJ
ajlm-15	10	4	manual	manual	ADJ
ajlm-15	10	5	assays	assay	NOUN
ajlm-15	10	6	,	,	PUNCT
ajlm-15	10	7	automated	automate	VERB
ajlm-15	10	8	elisa	elisa	NOUN
ajlm-15	10	9	systems	system	NOUN
ajlm-15	10	10	offer	offer	VERB
ajlm-15	10	11	more	more	ADV
ajlm-15	10	12	accurate	accurate	ADJ
ajlm-15	10	13	and	and	CCONJ
ajlm-15	10	14	reproducible	reproducible	ADJ
ajlm-15	10	15	results	result	NOUN
ajlm-15	10	16	,	,	PUNCT
ajlm-15	10	17	faster	fast	ADJ
ajlm-15	10	18	turnaround	turnaround	NOUN
ajlm-15	10	19	times	time	NOUN
ajlm-15	10	20	and	and	CCONJ
ajlm-15	10	21	cost	cost	NOUN
ajlm-15	10	22	effectiveness	effectiveness	NOUN
ajlm-15	10	23	due	due	ADP
ajlm-15	10	24	to	to	ADP
ajlm-15	10	25	the	the	DET
ajlm-15	10	26	use	use	NOUN
ajlm-15	10	27	of	of	ADP
ajlm-15	10	28	multianalyte	multianalyte	NOUN
ajlm-15	10	29	reagents.design	reagents.design	VERB
ajlm-15	10	30	:	:	PUNCT
ajlm-15	10	31	the	the	DET
ajlm-15	10	32	vacczyme	vacczyme	NOUN
ajlm-15	10	33	™	™	VERB
ajlm-15	10	34	human	human	ADJ
ajlm-15	10	35	anti	anti	ADJ
ajlm-15	10	36	-	-	ADJ
ajlm-15	10	37	haemophilus	haemophilus	ADJ
ajlm-15	10	38	influenzae	influenzae	ADJ
ajlm-15	10	39	type	type	NOUN
ajlm-15	10	40	b	b	PROPN
ajlm-15	10	41	(	(	PUNCT
ajlm-15	10	42	hib	hib	PROPN
ajlm-15	10	43	)	)	PUNCT
ajlm-15	10	44	kit	kit	NOUN
ajlm-15	10	45	(	(	PUNCT
ajlm-15	10	46	mk016	mk016	NOUN
ajlm-15	10	47	)	)	PUNCT
ajlm-15	10	48	from	from	ADP
ajlm-15	10	49	the	the	DET
ajlm-15	10	50	binding	bind	VERB
ajlm-15	10	51	site	site	NOUN
ajlm-15	10	52	company	company	NOUN
ajlm-15	10	53	was	be	AUX
ajlm-15	10	54	optimised	optimise	VERB
ajlm-15	10	55	to	to	PART
ajlm-15	10	56	be	be	AUX
ajlm-15	10	57	used	use	VERB
ajlm-15	10	58	on	on	ADP
ajlm-15	10	59	an	an	DET
ajlm-15	10	60	automated	automate	VERB
ajlm-15	10	61	biorad	biorad	ADJ
ajlm-15	10	62	phd	phd	NOUN
ajlm-15	10	63	™	™	ADJ
ajlm-15	10	64	system	system	NOUN
ajlm-15	10	65	in	in	ADP
ajlm-15	10	66	the	the	DET
ajlm-15	10	67	immunology	immunology	NOUN
ajlm-15	10	68	laboratory	laboratory	NOUN
ajlm-15	10	69	(	(	PUNCT
ajlm-15	10	70	national	national	PROPN
ajlm-15	10	71	health	health	PROPN
ajlm-15	10	72	laboratory	laboratory	PROPN
ajlm-15	10	73	service	service	PROPN
ajlm-15	10	74	)	)	PUNCT
ajlm-15	10	75	in	in	ADP
ajlm-15	10	76	tygerberg	tygerberg	PROPN
ajlm-15	10	77	,	,	PUNCT
ajlm-15	10	78	south	south	PROPN
ajlm-15	10	79	africa	africa	PROPN
ajlm-15	10	80	.	.	PUNCT
ajlm-15	11	1	methods	method	NOUN
ajlm-15	11	2	:	:	PUNCT
ajlm-15	11	3	an	an	DET
ajlm-15	11	4	automated	automate	VERB
ajlm-15	11	5	elisa	elisa	NOUN
ajlm-15	11	6	system	system	NOUN
ajlm-15	11	7	that	that	PRON
ajlm-15	11	8	uses	use	VERB
ajlm-15	11	9	individual	individual	ADJ
ajlm-15	12	1	well	well	ADJ
ajlm-15	12	2	incubation	incubation	NOUN
ajlm-15	12	3	was	be	AUX
ajlm-15	12	4	compared	compare	VERB
ajlm-15	12	5	to	to	ADP
ajlm-15	12	6	a	a	DET
ajlm-15	12	7	manual	manual	ADJ
ajlm-15	12	8	method	method	NOUN
ajlm-15	12	9	that	that	PRON
ajlm-15	12	10	uses	use	VERB
ajlm-15	12	11	whole	whole	ADJ
ajlm-15	12	12	-	-	PUNCT
ajlm-15	12	13	plate	plate	NOUN
ajlm-15	12	14	incubation	incubation	NOUN
ajlm-15	12	15	.	.	PUNCT
ajlm-15	13	1	results	result	NOUN
ajlm-15	13	2	:	:	PUNCT
ajlm-15	13	3	results	result	NOUN
ajlm-15	13	4	were	be	AUX
ajlm-15	13	5	calculated	calculate	VERB
ajlm-15	13	6	from	from	ADP
ajlm-15	13	7	calibration	calibration	NOUN
ajlm-15	13	8	curves	curve	NOUN
ajlm-15	13	9	constructed	construct	VERB
ajlm-15	13	10	with	with	ADP
ajlm-15	13	11	each	each	DET
ajlm-15	13	12	assay	assay	NOUN
ajlm-15	13	13	.	.	PUNCT
ajlm-15	14	1	marked	mark	VERB
ajlm-15	14	2	differences	difference	NOUN
ajlm-15	14	3	in	in	ADP
ajlm-15	14	4	calibration	calibration	NOUN
ajlm-15	14	5	curves	curve	NOUN
ajlm-15	14	6	were	be	AUX
ajlm-15	14	7	observed	observe	VERB
ajlm-15	14	8	for	for	ADP
ajlm-15	14	9	the	the	DET
ajlm-15	14	10	two	two	NUM
ajlm-15	14	11	methods	method	NOUN
ajlm-15	14	12	.	.	PUNCT
ajlm-15	15	1	the	the	DET
ajlm-15	15	2	automated	automate	VERB
ajlm-15	15	3	method	method	NOUN
ajlm-15	15	4	produced	produce	VERB
ajlm-15	15	5	lower	low	ADJ
ajlm-15	15	6	-	-	PUNCT
ajlm-15	15	7	than	than	ADP
ajlm-15	15	8	-	-	PUNCT
ajlm-15	15	9	recommended	recommend	VERB
ajlm-15	15	10	optical	optical	ADJ
ajlm-15	15	11	density	density	NOUN
ajlm-15	15	12	values	value	NOUN
ajlm-15	15	13	and	and	CCONJ
ajlm-15	15	14	resulted	result	VERB
ajlm-15	15	15	in	in	ADP
ajlm-15	15	16	invalid	invalid	ADJ
ajlm-15	15	17	calibration	calibration	NOUN
ajlm-15	15	18	curves	curve	NOUN
ajlm-15	15	19	and	and	CCONJ
ajlm-15	15	20	diagnostic	diagnostic	ADJ
ajlm-15	15	21	results	result	NOUN
ajlm-15	15	22	.	.	PUNCT
ajlm-15	16	1	a	a	DET
ajlm-15	16	2	comparison	comparison	NOUN
ajlm-15	16	3	of	of	ADP
ajlm-15	16	4	the	the	DET
ajlm-15	16	5	individual	individual	ADJ
ajlm-15	16	6	steps	step	NOUN
ajlm-15	16	7	of	of	ADP
ajlm-15	16	8	the	the	DET
ajlm-15	16	9	two	two	NUM
ajlm-15	16	10	methods	method	NOUN
ajlm-15	16	11	showed	show	VERB
ajlm-15	16	12	a	a	DET
ajlm-15	16	13	difference	difference	NOUN
ajlm-15	16	14	of	of	ADP
ajlm-15	16	15	10	10	NUM
ajlm-15	16	16	minutes	minute	NOUN
ajlm-15	16	17	per	per	ADP
ajlm-15	16	18	incubation	incubation	NOUN
ajlm-15	16	19	cycle	cycle	NOUN
ajlm-15	16	20	.	.	PUNCT
ajlm-15	17	1	all	all	DET
ajlm-15	17	2	incubation	incubation	NOUN
ajlm-15	17	3	steps	step	NOUN
ajlm-15	17	4	of	of	ADP
ajlm-15	17	5	the	the	DET
ajlm-15	17	6	automated	automate	VERB
ajlm-15	17	7	method	method	NOUN
ajlm-15	17	8	were	be	AUX
ajlm-15	17	9	subsequently	subsequently	ADV
ajlm-15	17	10	increased	increase	VERB
ajlm-15	17	11	from	from	ADP
ajlm-15	17	12	30	30	NUM
ajlm-15	17	13	minutes	minute	NOUN
ajlm-15	17	14	to	to	ADP
ajlm-15	17	15	40	40	NUM
ajlm-15	17	16	minutes	minute	NOUN
ajlm-15	17	17	.	.	PUNCT
ajlm-15	18	1	several	several	ADJ
ajlm-15	18	2	comparative	comparative	ADJ
ajlm-15	18	3	assays	assay	NOUN
ajlm-15	18	4	were	be	AUX
ajlm-15	18	5	performed	perform	VERB
ajlm-15	18	6	according	accord	VERB
ajlm-15	18	7	to	to	ADP
ajlm-15	18	8	the	the	DET
ajlm-15	18	9	amended	amend	VERB
ajlm-15	18	10	protocol	protocol	NOUN
ajlm-15	18	11	and	and	CCONJ
ajlm-15	18	12	all	all	DET
ajlm-15	18	13	calibration	calibration	NOUN
ajlm-15	18	14	curves	curve	NOUN
ajlm-15	18	15	obtained	obtain	VERB
ajlm-15	18	16	were	be	AUX
ajlm-15	18	17	valid	valid	ADJ
ajlm-15	18	18	.	.	PUNCT
ajlm-15	19	1	calibrators	calibrator	NOUN
ajlm-15	19	2	and	and	CCONJ
ajlm-15	19	3	controls	control	NOUN
ajlm-15	19	4	were	be	AUX
ajlm-15	19	5	also	also	ADV
ajlm-15	19	6	included	include	VERB
ajlm-15	19	7	as	as	ADP
ajlm-15	19	8	samples	sample	NOUN
ajlm-15	19	9	in	in	ADP
ajlm-15	19	10	different	different	ADJ
ajlm-15	19	11	positions	position	NOUN
ajlm-15	19	12	and	and	CCONJ
ajlm-15	19	13	orders	order	NOUN
ajlm-15	19	14	on	on	ADP
ajlm-15	19	15	the	the	DET
ajlm-15	19	16	plate	plate	NOUN
ajlm-15	19	17	and	and	CCONJ
ajlm-15	19	18	all	all	DET
ajlm-15	19	19	results	result	NOUN
ajlm-15	19	20	were	be	AUX
ajlm-15	19	21	valid	valid	ADJ
ajlm-15	19	22	.	.	PUNCT
ajlm-15	20	1	conclusion	conclusion	NOUN
ajlm-15	20	2	:	:	PUNCT
ajlm-15	20	3	proper	proper	ADJ
ajlm-15	20	4	validation	validation	NOUN
ajlm-15	20	5	is	be	AUX
ajlm-15	20	6	vital	vital	ADJ
ajlm-15	20	7	before	before	ADP
ajlm-15	20	8	converting	convert	VERB
ajlm-15	20	9	manual	manual	ADJ
ajlm-15	20	10	elisa	elisa	VERB
ajlm-15	20	11	assays	assay	NOUN
ajlm-15	20	12	to	to	ADP
ajlm-15	20	13	automated	automate	VERB
ajlm-15	20	14	or	or	CCONJ
ajlm-15	20	15	semi	semi	ADJ
ajlm-15	20	16	-	-	ADJ
ajlm-15	20	17	automated	automated	ADJ
ajlm-15	20	18	methods	method	NOUN
ajlm-15	20	19	.	.	PUNCT
ajlm-15	21	1	introduction	introduction	NOUN
ajlm-15	21	2	top	top	PROPN
ajlm-15	21	3	↑	↑	PROPN
ajlm-15	21	4	quantitative	quantitative	ADJ
ajlm-15	21	5	analytical	analytical	ADJ
ajlm-15	21	6	methods	method	NOUN
ajlm-15	21	7	have	have	AUX
ajlm-15	21	8	advanced	advance	VERB
ajlm-15	21	9	significantly	significantly	ADV
ajlm-15	21	10	since	since	SCONJ
ajlm-15	21	11	the	the	DET
ajlm-15	21	12	development	development	NOUN
ajlm-15	21	13	of	of	ADP
ajlm-15	21	14	the	the	DET
ajlm-15	21	15	enzyme	enzyme	NOUN
ajlm-15	21	16	immunoassay	immunoassay	NOUN
ajlm-15	21	17	(	(	PUNCT
ajlm-15	21	18	eia	eia	PROPN
ajlm-15	21	19	)	)	PUNCT
ajlm-15	21	20	and	and	CCONJ
ajlm-15	21	21	enzyme	enzyme	NOUN
ajlm-15	21	22	-	-	PUNCT
ajlm-15	21	23	linked	link	VERB
ajlm-15	21	24	immunosorbent	immunosorbent	NOUN
ajlm-15	21	25	assay	assay	PROPN
ajlm-15	21	26	(	(	PUNCT
ajlm-15	21	27	elisa	elisa	NOUN
ajlm-15	21	28	)	)	PUNCT
ajlm-15	21	29	in	in	ADP
ajlm-15	21	30	1971	1971	NUM
ajlm-15	21	31	by	by	ADP
ajlm-15	21	32	the	the	DET
ajlm-15	21	33	groups	group	NOUN
ajlm-15	21	34	of	of	ADP
ajlm-15	21	35	perlmann	perlmann	PROPN
ajlm-15	21	36	and	and	CCONJ
ajlm-15	21	37	engvall	engvall	PROPN
ajlm-15	21	38	,	,	PUNCT
ajlm-15	21	39	and	and	CCONJ
ajlm-15	21	40	schuurs	schuur	VERB
ajlm-15	21	41	and	and	CCONJ
ajlm-15	21	42	van	van	PROPN
ajlm-15	21	43	weemen	weeman	NOUN
ajlm-15	21	44	,	,	PUNCT
ajlm-15	21	45	respectively.1,2,3	respectively.1,2,3	VERB
ajlm-15	21	46	before	before	ADP
ajlm-15	21	47	this	this	PRON
ajlm-15	21	48	,	,	PUNCT
ajlm-15	21	49	the	the	DET
ajlm-15	21	50	only	only	ADJ
ajlm-15	21	51	method	method	NOUN
ajlm-15	21	52	for	for	ADP
ajlm-15	21	53	performing	perform	VERB
ajlm-15	21	54	immunoassays	immunoassay	NOUN
ajlm-15	21	55	was	be	AUX
ajlm-15	21	56	the	the	DET
ajlm-15	21	57	radioimmunoassay	radioimmunoassay	ADJ
ajlm-15	21	58	(	(	PUNCT
ajlm-15	21	59	ria	ria	PROPN
ajlm-15	21	60	)	)	PUNCT
ajlm-15	21	61	,	,	PUNCT
ajlm-15	21	62	which	which	PRON
ajlm-15	21	63	was	be	AUX
ajlm-15	21	64	first	first	ADV
ajlm-15	21	65	described	describe	VERB
ajlm-15	21	66	by	by	ADP
ajlm-15	21	67	yalow	yalow	NOUN
ajlm-15	21	68	and	and	CCONJ
ajlm-15	21	69	berson	berson	NOUN
ajlm-15	21	70	in	in	ADP
ajlm-15	21	71	1960.4however	1960.4however	NUM
ajlm-15	21	72	,	,	PUNCT
ajlm-15	21	73	the	the	DET
ajlm-15	21	74	ria	ria	PROPN
ajlm-15	21	75	had	have	VERB
ajlm-15	21	76	several	several	ADJ
ajlm-15	21	77	shortcomings	shortcoming	NOUN
ajlm-15	21	78	,	,	PUNCT
ajlm-15	21	79	for	for	ADP
ajlm-15	21	80	example	example	NOUN
ajlm-15	21	81	the	the	DET
ajlm-15	21	82	potential	potential	ADJ
ajlm-15	21	83	health	health	NOUN
ajlm-15	21	84	threat	threat	NOUN
ajlm-15	21	85	of	of	ADP
ajlm-15	21	86	radioactivity	radioactivity	NOUN
ajlm-15	21	87	,	,	PUNCT
ajlm-15	21	88	short	short	ADJ
ajlm-15	21	89	half	half	ADJ
ajlm-15	21	90	-	-	PUNCT
ajlm-15	21	91	lives	life	NOUN
ajlm-15	21	92	of	of	ADP
ajlm-15	21	93	radioisotopes	radioisotope	NOUN
ajlm-15	21	94	,	,	PUNCT
ajlm-15	21	95	cumbersome	cumbersome	ADJ
ajlm-15	21	96	radioactive	radioactive	ADJ
ajlm-15	21	97	waste	waste	NOUN
ajlm-15	21	98	disposal	disposal	NOUN
ajlm-15	21	99	,	,	PUNCT
ajlm-15	21	100	expensive	expensive	ADJ
ajlm-15	21	101	counting	counting	NOUN
ajlm-15	21	102	equipment	equipment	NOUN
ajlm-15	21	103	,	,	PUNCT
ajlm-15	21	104	etc.3,5,6	etc.3,5,6	VERB
ajlm-15	21	105	an	an	DET
ajlm-15	21	106	important	important	ADJ
ajlm-15	21	107	shift	shift	NOUN
ajlm-15	21	108	from	from	ADP
ajlm-15	21	109	radioisotope	radioisotope	NOUN
ajlm-15	21	110	-	-	PUNCT
ajlm-15	21	111	labelled	label	VERB
ajlm-15	21	112	liquid	liquid	NOUN
ajlm-15	21	113	-	-	PUNCT
ajlm-15	21	114	phase	phase	NOUN
ajlm-15	21	115	assays	assay	NOUN
ajlm-15	21	116	to	to	ADP
ajlm-15	21	117	solid	solid	ADJ
ajlm-15	21	118	-	-	PUNCT
ajlm-15	21	119	phase	phase	NOUN
ajlm-15	21	120	assays	assay	NOUN
ajlm-15	21	121	occurred	occur	VERB
ajlm-15	21	122	in	in	ADP
ajlm-15	21	123	1968	1968	NUM
ajlm-15	21	124	.	.	PUNCT
ajlm-15	22	1	miles	mile	NOUN
ajlm-15	22	2	and	and	CCONJ
ajlm-15	22	3	hales7	hales7	ADV
ajlm-15	22	4	developed	develop	VERB
ajlm-15	22	5	an	an	DET
ajlm-15	22	6	immuno	immuno	ADJ
ajlm-15	22	7	-	-	PUNCT
ajlm-15	22	8	radiometric	radiometric	ADJ
ajlm-15	22	9	technique	technique	NOUN
ajlm-15	22	10	,	,	PUNCT
ajlm-15	22	11	which	which	PRON
ajlm-15	22	12	used	use	VERB
ajlm-15	22	13	radioactively	radioactively	NOUN
ajlm-15	22	14	labelled	label	VERB
ajlm-15	22	15	antibodies	antibody	NOUN
ajlm-15	22	16	instead	instead	ADV
ajlm-15	22	17	of	of	ADP
ajlm-15	22	18	labelled	label	VERB
ajlm-15	22	19	antigens	antigen	NOUN
ajlm-15	22	20	for	for	ADP
ajlm-15	22	21	measuring	measure	VERB
ajlm-15	22	22	insulin	insulin	NOUN
ajlm-15	22	23	in	in	ADP
ajlm-15	22	24	human	human	ADJ
ajlm-15	22	25	plasma	plasma	NOUN
ajlm-15	22	26	.	.	PUNCT
ajlm-15	23	1	plastic	plastic	NOUN
ajlm-15	23	2	tubes	tube	NOUN
ajlm-15	23	3	were	be	AUX
ajlm-15	23	4	subsequently	subsequently	ADV
ajlm-15	23	5	coated	coat	VERB
ajlm-15	23	6	with	with	ADP
ajlm-15	23	7	the	the	DET
ajlm-15	23	8	antigen	antigen	NOUN
ajlm-15	23	9	or	or	CCONJ
ajlm-15	23	10	antibody	antibody	NOUN
ajlm-15	23	11	to	to	PART
ajlm-15	23	12	create	create	VERB
ajlm-15	23	13	a	a	DET
ajlm-15	23	14	solid	solid	ADJ
ajlm-15	23	15	-	-	PUNCT
ajlm-15	23	16	phase	phase	NOUN
ajlm-15	23	17	or	or	CCONJ
ajlm-15	23	18	immunosorbent	immunosorbent	VERB
ajlm-15	23	19	platform	platform	NOUN
ajlm-15	23	20	.	.	PUNCT
ajlm-15	24	1	8	8	NUM
ajlm-15	24	2	modern	modern	ADJ
ajlm-15	24	3	commercial	commercial	ADJ
ajlm-15	24	4	elisa	elisa	PROPN
ajlm-15	24	5	/	/	SYM
ajlm-15	24	6	eia	eia	PROPN
ajlm-15	24	7	kits	kit	NOUN
ajlm-15	24	8	use	use	VERB
ajlm-15	24	9	96	96	NUM
ajlm-15	24	10	-	-	PUNCT
ajlm-15	24	11	well	well	NOUN
ajlm-15	24	12	microtitre	microtitre	NOUN
ajlm-15	24	13	plates	plate	NOUN
ajlm-15	24	14	,	,	PUNCT
ajlm-15	24	15	where	where	SCONJ
ajlm-15	24	16	either	either	CCONJ
ajlm-15	24	17	an	an	DET
ajlm-15	24	18	antigen	antigen	NOUN
ajlm-15	24	19	or	or	CCONJ
ajlm-15	24	20	an	an	DET
ajlm-15	24	21	antibody	antibody	NOUN
ajlm-15	24	22	is	be	AUX
ajlm-15	24	23	noncovalently	noncovalently	ADV
ajlm-15	24	24	bound	bind	VERB
ajlm-15	24	25	to	to	ADP
ajlm-15	24	26	a	a	DET
ajlm-15	24	27	solid	solid	ADJ
ajlm-15	24	28	-	-	PUNCT
ajlm-15	24	29	phase	phase	NOUN
ajlm-15	24	30	support	support	NOUN
ajlm-15	24	31	.	.	PUNCT
ajlm-15	25	1	these	these	DET
ajlm-15	25	2	methods	method	NOUN
ajlm-15	25	3	are	be	AUX
ajlm-15	25	4	widely	widely	ADV
ajlm-15	25	5	employed	employ	VERB
ajlm-15	25	6	by	by	ADP
ajlm-15	25	7	laboratories	laboratory	NOUN
ajlm-15	25	8	and	and	CCONJ
ajlm-15	25	9	manufacturing	manufacturing	NOUN
ajlm-15	25	10	companies	company	NOUN
ajlm-15	25	11	for	for	ADP
ajlm-15	25	12	microbiological	microbiological	ADJ
ajlm-15	25	13	,	,	PUNCT
ajlm-15	25	14	virological	virological	ADJ
ajlm-15	25	15	and	and	CCONJ
ajlm-15	25	16	other	other	ADJ
ajlm-15	25	17	serological	serological	ADJ
ajlm-15	25	18	diagnostic	diagnostic	ADJ
ajlm-15	25	19	tests	test	NOUN
ajlm-15	25	20	,	,	PUNCT
ajlm-15	25	21	validation	validation	NOUN
ajlm-15	25	22	of	of	ADP
ajlm-15	25	23	assays	assay	NOUN
ajlm-15	25	24	and	and	CCONJ
ajlm-15	25	25	general	general	ADJ
ajlm-15	25	26	quality	quality	NOUN
ajlm-15	25	27	control	control	NOUN
ajlm-15	25	28	.	.	PUNCT
ajlm-15	26	1	although	although	SCONJ
ajlm-15	26	2	automated	automate	VERB
ajlm-15	26	3	pipetting	pipetting	NOUN
ajlm-15	26	4	devices	device	NOUN
ajlm-15	26	5	have	have	AUX
ajlm-15	26	6	been	be	AUX
ajlm-15	26	7	used	use	VERB
ajlm-15	26	8	for	for	ADP
ajlm-15	26	9	more	more	ADJ
ajlm-15	26	10	than	than	ADP
ajlm-15	26	11	two	two	NUM
ajlm-15	26	12	decades	decade	NOUN
ajlm-15	26	13	,	,	PUNCT
ajlm-15	26	14	the	the	DET
ajlm-15	26	15	high	high	ADJ
ajlm-15	26	16	cost	cost	NOUN
ajlm-15	26	17	associated	associate	VERB
ajlm-15	26	18	with	with	ADP
ajlm-15	26	19	the	the	DET
ajlm-15	26	20	technique	technique	NOUN
ajlm-15	26	21	remains	remain	VERB
ajlm-15	26	22	a	a	DET
ajlm-15	26	23	major	major	ADJ
ajlm-15	26	24	limiting	limit	VERB
ajlm-15	26	25	factor	factor	NOUN
ajlm-15	26	26	in	in	ADP
ajlm-15	26	27	developing	develop	VERB
ajlm-15	26	28	countries	country	NOUN
ajlm-15	26	29	and	and	CCONJ
ajlm-15	26	30	smaller	small	ADJ
ajlm-15	26	31	laboratories.3	laboratories.3	NOUN
ajlm-15	26	32	materials	material	NOUN
ajlm-15	26	33	and	and	CCONJ
ajlm-15	26	34	methods	method	NOUN
ajlm-15	26	35	top	top	VERB
ajlm-15	26	36	↑	↑	PROPN
ajlm-15	26	37	the	the	DET
ajlm-15	26	38	immunology	immunology	NOUN
ajlm-15	26	39	unit	unit	NOUN
ajlm-15	26	40	of	of	ADP
ajlm-15	26	41	the	the	DET
ajlm-15	26	42	national	national	PROPN
ajlm-15	26	43	health	health	PROPN
ajlm-15	26	44	laboratory	laboratory	PROPN
ajlm-15	26	45	service	service	NOUN
ajlm-15	26	46	(	(	PUNCT
ajlm-15	26	47	nhls	nhls	PROPN
ajlm-15	26	48	)	)	PUNCT
ajlm-15	26	49	in	in	ADP
ajlm-15	26	50	tygerberg	tygerberg	PROPN
ajlm-15	26	51	,	,	PUNCT
ajlm-15	26	52	south	south	PROPN
ajlm-15	26	53	africa	africa	PROPN
ajlm-15	26	54	,	,	PUNCT
ajlm-15	26	55	uses	use	VERB
ajlm-15	26	56	elisas	elisa	NOUN
ajlm-15	26	57	for	for	ADP
ajlm-15	26	58	serological	serological	ADJ
ajlm-15	26	59	quantification	quantification	NOUN
ajlm-15	26	60	of	of	ADP
ajlm-15	26	61	antibody	antibody	NOUN
ajlm-15	26	62	levels	level	NOUN
ajlm-15	26	63	.	.	PUNCT
ajlm-15	27	1	before	before	ADP
ajlm-15	27	2	installation	installation	NOUN
ajlm-15	27	3	of	of	ADP
ajlm-15	27	4	the	the	DET
ajlm-15	27	5	biorad	biorad	ADJ
ajlm-15	27	6	phd	phd	NOUN
ajlm-15	27	7	™	™	ADJ
ajlm-15	27	8	system	system	NOUN
ajlm-15	27	9	,	,	PUNCT
ajlm-15	27	10	all	all	DET
ajlm-15	27	11	assays	assay	NOUN
ajlm-15	27	12	had	have	AUX
ajlm-15	27	13	been	be	AUX
ajlm-15	27	14	performed	perform	VERB
ajlm-15	27	15	manually	manually	ADV
ajlm-15	27	16	.	.	PUNCT
ajlm-15	28	1	the	the	DET
ajlm-15	28	2	system	system	NOUN
ajlm-15	28	3	performs	perform	VERB
ajlm-15	28	4	sample	sample	NOUN
ajlm-15	28	5	dilution	dilution	NOUN
ajlm-15	28	6	,	,	PUNCT
ajlm-15	28	7	dispenses	dispense	VERB
ajlm-15	28	8	patient	patient	ADJ
ajlm-15	28	9	samples	sample	NOUN
ajlm-15	28	10	and	and	CCONJ
ajlm-15	28	11	reagents	reagent	NOUN
ajlm-15	28	12	into	into	ADP
ajlm-15	28	13	the	the	DET
ajlm-15	28	14	microplate	microplate	NOUN
ajlm-15	28	15	,	,	PUNCT
ajlm-15	28	16	and	and	CCONJ
ajlm-15	28	17	performs	perform	VERB
ajlm-15	28	18	temperature	temperature	NOUN
ajlm-15	28	19	-	-	PUNCT
ajlm-15	28	20	specific	specific	ADJ
ajlm-15	28	21	incubation	incubation	NOUN
ajlm-15	28	22	and	and	CCONJ
ajlm-15	28	23	washing	washing	NOUN
ajlm-15	28	24	according	accord	VERB
ajlm-15	28	25	to	to	ADP
ajlm-15	28	26	pre	pre	ADJ
ajlm-15	28	27	-	-	ADJ
ajlm-15	28	28	defined	define	VERB
ajlm-15	28	29	protocols	protocol	NOUN
ajlm-15	28	30	.	.	PUNCT
ajlm-15	29	1	automation	automation	NOUN
ajlm-15	29	2	of	of	ADP
ajlm-15	29	3	the	the	DET
ajlm-15	29	4	mk016	mk016	VERB
ajlm-15	29	5	enzyme	enzyme	NOUN
ajlm-15	29	6	-	-	PUNCT
ajlm-15	29	7	linked	link	VERB
ajlm-15	29	8	immunosorbent	immunosorbent	NOUN
ajlm-15	29	9	assay	assay	VERB
ajlm-15	29	10	the	the	DET
ajlm-15	29	11	96	96	NUM
ajlm-15	29	12	-	-	PUNCT
ajlm-15	29	13	well	well	NOUN
ajlm-15	29	14	microtitre	microtitre	NOUN
ajlm-15	29	15	plate	plate	NOUN
ajlm-15	29	16	included	include	VERB
ajlm-15	29	17	in	in	ADP
ajlm-15	29	18	the	the	DET
ajlm-15	29	19	vacczyme	vacczyme	NOUN
ajlm-15	29	20	™	™	VERB
ajlm-15	29	21	human	human	ADJ
ajlm-15	29	22	anti	anti	ADJ
ajlm-15	29	23	-	-	ADJ
ajlm-15	29	24	haemophilus	haemophilus	ADJ
ajlm-15	29	25	influenzae	influenzae	ADJ
ajlm-15	29	26	type	type	NOUN
ajlm-15	29	27	b	b	PROPN
ajlm-15	29	28	(	(	PUNCT
ajlm-15	29	29	hib	hib	PROPN
ajlm-15	29	30	)	)	PUNCT
ajlm-15	29	31	kit	kit	NOUN
ajlm-15	29	32	(	(	PUNCT
ajlm-15	29	33	mk016	mk016	NOUN
ajlm-15	29	34	)	)	PUNCT
ajlm-15	29	35	from	from	ADP
ajlm-15	29	36	the	the	DET
ajlm-15	29	37	binding	bind	VERB
ajlm-15	29	38	site	site	NOUN
ajlm-15	29	39	company	company	NOUN
ajlm-15	29	40	(	(	PUNCT
ajlm-15	29	41	birmingham	birmingham	PROPN
ajlm-15	29	42	,	,	PUNCT
ajlm-15	29	43	united	united	ADJ
ajlm-15	29	44	kingdom	kingdom	PROPN
ajlm-15	29	45	)	)	PUNCT
ajlm-15	29	46	is	be	AUX
ajlm-15	29	47	precoated	precoate	VERB
ajlm-15	29	48	with	with	ADP
ajlm-15	29	49	the	the	DET
ajlm-15	29	50	hib	hib	NOUN
ajlm-15	29	51	capsular	capsular	VERB
ajlm-15	29	52	polysaccharide	polysaccharide	NOUN
ajlm-15	29	53	antigen	antigen	NOUN
ajlm-15	29	54	conjugated	conjugate	VERB
ajlm-15	29	55	to	to	ADP
ajlm-15	29	56	human	human	ADJ
ajlm-15	29	57	serum	serum	NOUN
ajlm-15	29	58	albumin	albumin	NOUN
ajlm-15	29	59	.	.	PUNCT
ajlm-15	30	1	in	in	ADP
ajlm-15	30	2	addition	addition	NOUN
ajlm-15	30	3	to	to	ADP
ajlm-15	30	4	controls	control	NOUN
ajlm-15	30	5	and	and	CCONJ
ajlm-15	30	6	other	other	ADJ
ajlm-15	30	7	reagents	reagent	NOUN
ajlm-15	30	8	,	,	PUNCT
ajlm-15	30	9	the	the	DET
ajlm-15	30	10	kit	kit	NOUN
ajlm-15	30	11	also	also	ADV
ajlm-15	30	12	contains	contain	VERB
ajlm-15	30	13	five	five	NUM
ajlm-15	30	14	calibrators	calibrator	NOUN
ajlm-15	30	15	(	(	PUNCT
ajlm-15	30	16	0.1	0.1	NUM
ajlm-15	30	17	mg	mg	PROPN
ajlm-15	30	18	/	/	SYM
ajlm-15	30	19	l	l	NOUN
ajlm-15	30	20	–	–	PUNCT
ajlm-15	30	21	9.0	9.0	NUM
ajlm-15	30	22	 	 	SPACE
ajlm-15	30	23	mg	mg	PROPN
ajlm-15	30	24	/	/	SYM
ajlm-15	30	25	l	l	NOUN
ajlm-15	30	26	)	)	PUNCT
ajlm-15	30	27	to	to	PART
ajlm-15	30	28	construct	construct	VERB
ajlm-15	30	29	a	a	DET
ajlm-15	30	30	five	five	NUM
ajlm-15	30	31	-	-	PUNCT
ajlm-15	30	32	point	point	NOUN
ajlm-15	30	33	calibration	calibration	NOUN
ajlm-15	30	34	curve	curve	NOUN
ajlm-15	30	35	.	.	PUNCT
ajlm-15	31	1	concentration	concentration	NOUN
ajlm-15	31	2	(	(	PUNCT
ajlm-15	31	3	logarithmic	logarithmic	ADJ
ajlm-15	31	4	scale	scale	NOUN
ajlm-15	31	5	)	)	PUNCT
ajlm-15	31	6	is	be	AUX
ajlm-15	31	7	plotted	plot	VERB
ajlm-15	31	8	against	against	ADP
ajlm-15	31	9	optical	optical	ADJ
ajlm-15	31	10	density	density	NOUN
ajlm-15	31	11	(	(	PUNCT
ajlm-15	31	12	linear	linear	ADJ
ajlm-15	31	13	scale	scale	NOUN
ajlm-15	31	14	)	)	PUNCT
ajlm-15	31	15	to	to	PART
ajlm-15	31	16	produce	produce	VERB
ajlm-15	31	17	the	the	DET
ajlm-15	31	18	calibration	calibration	NOUN
ajlm-15	31	19	curve	curve	NOUN
ajlm-15	31	20	.	.	PUNCT
ajlm-15	32	1	the	the	DET
ajlm-15	32	2	quantification	quantification	NOUN
ajlm-15	32	3	range	range	NOUN
ajlm-15	32	4	for	for	ADP
ajlm-15	32	5	anti	anti	ADJ
ajlm-15	32	6	-	-	ADJ
ajlm-15	32	7	hib	hib	ADJ
ajlm-15	32	8	antibody	antibody	NOUN
ajlm-15	32	9	concentration	concentration	NOUN
ajlm-15	32	10	is	be	AUX
ajlm-15	32	11	0.11	0.11	NUM
ajlm-15	32	12	mg	mg	PROPN
ajlm-15	32	13	/	/	SYM
ajlm-15	32	14	l	l	NOUN
ajlm-15	32	15	–	–	PUNCT
ajlm-15	32	16	9.0	9.0	NUM
ajlm-15	32	17	 	 	SPACE
ajlm-15	32	18	mg	mg	PROPN
ajlm-15	32	19	/	/	SYM
ajlm-15	32	20	l.9prediluted	l.9predilute	VERB
ajlm-15	32	21	samples	sample	NOUN
ajlm-15	32	22	and	and	CCONJ
ajlm-15	32	23	controls	control	NOUN
ajlm-15	32	24	were	be	AUX
ajlm-15	32	25	pipetted	pipette	VERB
ajlm-15	32	26	into	into	ADP
ajlm-15	32	27	the	the	DET
ajlm-15	32	28	plate	plate	NOUN
ajlm-15	32	29	and	and	CCONJ
ajlm-15	32	30	incubated	incubate	VERB
ajlm-15	32	31	for	for	ADP
ajlm-15	32	32	30	30	NUM
ajlm-15	32	33	minutes	minute	NOUN
ajlm-15	32	34	.	.	PUNCT
ajlm-15	33	1	unbound	unbound	NOUN
ajlm-15	33	2	proteins	protein	NOUN
ajlm-15	33	3	were	be	AUX
ajlm-15	33	4	removed	remove	VERB
ajlm-15	33	5	by	by	ADP
ajlm-15	33	6	a	a	DET
ajlm-15	33	7	wash	wash	NOUN
ajlm-15	33	8	step	step	NOUN
ajlm-15	33	9	before	before	ADP
ajlm-15	33	10	addition	addition	NOUN
ajlm-15	33	11	of	of	ADP
ajlm-15	33	12	conjugate	conjugate	ADJ
ajlm-15	33	13	(	(	PUNCT
ajlm-15	33	14	purified	purified	ADJ
ajlm-15	33	15	peroxidase	peroxidase	NOUN
ajlm-15	33	16	-	-	PUNCT
ajlm-15	33	17	labelled	label	VERB
ajlm-15	33	18	rabbit	rabbit	NOUN
ajlm-15	33	19	anti	anti	ADJ
ajlm-15	33	20	-	-	ADJ
ajlm-15	33	21	human	human	ADJ
ajlm-15	33	22	g	g	NOUN
ajlm-15	33	23	-	-	PUNCT
ajlm-15	33	24	chain	chain	NOUN
ajlm-15	33	25	-	-	PUNCT
ajlm-15	33	26	specific	specific	ADJ
ajlm-15	33	27	immunoglobulin	immunoglobulin	NOUN
ajlm-15	33	28	g	g	NOUN
ajlm-15	33	29	)	)	PUNCT
ajlm-15	33	30	.	.	PUNCT
ajlm-15	34	1	after	after	ADP
ajlm-15	34	2	a	a	DET
ajlm-15	34	3	further	further	ADJ
ajlm-15	34	4	30	30	NUM
ajlm-15	34	5	minutes	minute	NOUN
ajlm-15	34	6	of	of	ADP
ajlm-15	34	7	incubation	incubation	NOUN
ajlm-15	34	8	,	,	PUNCT
ajlm-15	34	9	another	another	DET
ajlm-15	34	10	wash	wash	NOUN
ajlm-15	34	11	step	step	NOUN
ajlm-15	34	12	was	be	AUX
ajlm-15	34	13	applied	apply	VERB
ajlm-15	34	14	to	to	PART
ajlm-15	34	15	remove	remove	VERB
ajlm-15	34	16	all	all	DET
ajlm-15	34	17	excess	excess	ADJ
ajlm-15	34	18	conjugate	conjugate	NOUN
ajlm-15	34	19	.	.	PUNCT
ajlm-15	35	1	substrate	substrate	NOUN
ajlm-15	35	2	(	(	PUNCT
ajlm-15	35	3	3.3’,5.5	3.3’,5.5	NUM
ajlm-15	35	4	’	'	PUNCT
ajlm-15	35	5	tetramethylbenzidine	tetramethylbenzidine	NOUN
ajlm-15	35	6	)	)	PUNCT
ajlm-15	35	7	was	be	AUX
ajlm-15	35	8	then	then	ADV
ajlm-15	35	9	added	add	VERB
ajlm-15	35	10	,	,	PUNCT
ajlm-15	35	11	which	which	PRON
ajlm-15	35	12	induced	induce	VERB
ajlm-15	35	13	a	a	DET
ajlm-15	35	14	colour	colour	NOUN
ajlm-15	35	15	change	change	NOUN
ajlm-15	35	16	(	(	PUNCT
ajlm-15	35	17	from	from	ADP
ajlm-15	35	18	the	the	DET
ajlm-15	35	19	characteristic	characteristic	ADJ
ajlm-15	35	20	serum	serum	NOUN
ajlm-15	35	21	colour	colour	NOUN
ajlm-15	35	22	to	to	PART
ajlm-15	35	23	blue	blue	VERB
ajlm-15	35	24	)	)	PUNCT
ajlm-15	35	25	over	over	ADP
ajlm-15	35	26	30	30	NUM
ajlm-15	35	27	minutes	minute	NOUN
ajlm-15	35	28	.	.	PUNCT
ajlm-15	36	1	phosphoric	phosphoric	PROPN
ajlm-15	36	2	acid	acid	PROPN
ajlm-15	36	3	was	be	AUX
ajlm-15	36	4	then	then	ADV
ajlm-15	36	5	added	add	VERB
ajlm-15	36	6	to	to	PART
ajlm-15	36	7	stop	stop	VERB
ajlm-15	36	8	colour	colour	NOUN
ajlm-15	36	9	development	development	NOUN
ajlm-15	36	10	.	.	PUNCT
ajlm-15	37	1	the	the	DET
ajlm-15	37	2	optical	optical	ADJ
ajlm-15	37	3	density	density	NOUN
ajlm-15	37	4	(	(	PUNCT
ajlm-15	37	5	od	od	NOUN
ajlm-15	37	6	)	)	PUNCT
ajlm-15	37	7	was	be	AUX
ajlm-15	37	8	measured	measure	VERB
ajlm-15	37	9	spectrophotometrically	spectrophotometrically	ADV
ajlm-15	37	10	at	at	ADP
ajlm-15	37	11	450	450	NUM
ajlm-15	37	12	 	 	SPACE
ajlm-15	37	13	nm	nm	NOUN
ajlm-15	37	14	and	and	CCONJ
ajlm-15	37	15	the	the	DET
ajlm-15	37	16	intensity	intensity	NOUN
ajlm-15	37	17	of	of	ADP
ajlm-15	37	18	the	the	DET
ajlm-15	37	19	final	final	ADJ
ajlm-15	37	20	colour	colour	NOUN
ajlm-15	37	21	is	be	AUX
ajlm-15	37	22	proportional	proportional	ADJ
ajlm-15	37	23	to	to	ADP
ajlm-15	37	24	the	the	DET
ajlm-15	37	25	concentration	concentration	NOUN
ajlm-15	37	26	of	of	ADP
ajlm-15	37	27	antibody	antibody	NOUN
ajlm-15	37	28	present	present	ADJ
ajlm-15	37	29	in	in	ADP
ajlm-15	37	30	the	the	DET
ajlm-15	37	31	sample	sample	NOUN
ajlm-15	37	32	.	.	PUNCT
ajlm-15	38	1	a	a	DET
ajlm-15	38	2	list	list	NOUN
ajlm-15	38	3	of	of	ADP
ajlm-15	38	4	assays	assay	NOUN
ajlm-15	38	5	that	that	PRON
ajlm-15	38	6	are	be	AUX
ajlm-15	38	7	validated	validate	VERB
ajlm-15	38	8	on	on	ADP
ajlm-15	38	9	the	the	DET
ajlm-15	38	10	phd	phd	NOUN
ajlm-15	38	11	™	™	ADJ
ajlm-15	38	12	system	system	NOUN
ajlm-15	38	13	is	be	AUX
ajlm-15	38	14	available	available	ADJ
ajlm-15	38	15	from	from	ADP
ajlm-15	38	16	biorad	biorad	NOUN
ajlm-15	38	17	(	(	PUNCT
ajlm-15	38	18	www.bio-rad.com	www.bio-rad.com	X
ajlm-15	38	19	;	;	PUNCT
ajlm-15	38	20	phd	phd	NOUN
ajlm-15	38	21	™	™	PROPN
ajlm-15	38	22	validated	validate	VERB
ajlm-15	38	23	assay	assay	NOUN
ajlm-15	38	24	list	list	NOUN
ajlm-15	38	25	)	)	PUNCT
ajlm-15	38	26	.	.	PUNCT
ajlm-15	39	1	results	result	VERB
ajlm-15	39	2	top	top	ADJ
ajlm-15	39	3	↑	↑	PROPN
ajlm-15	39	4	analyses	analysis	NOUN
ajlm-15	39	5	with	with	ADP
ajlm-15	39	6	elisa	elisa	PROPN
ajlm-15	39	7	kits	kit	NOUN
ajlm-15	39	8	from	from	ADP
ajlm-15	39	9	the	the	DET
ajlm-15	39	10	biorad	biorad	NOUN
ajlm-15	39	11	list	list	NOUN
ajlm-15	39	12	produced	produce	VERB
ajlm-15	39	13	valid	valid	ADJ
ajlm-15	39	14	results	result	NOUN
ajlm-15	39	15	in	in	ADP
ajlm-15	39	16	our	our	PRON
ajlm-15	39	17	laboratory	laboratory	NOUN
ajlm-15	39	18	and	and	CCONJ
ajlm-15	39	19	these	these	DET
ajlm-15	39	20	methods	method	NOUN
ajlm-15	39	21	were	be	AUX
ajlm-15	39	22	automated	automate	VERB
ajlm-15	39	23	without	without	ADP
ajlm-15	39	24	any	any	DET
ajlm-15	39	25	problems	problem	NOUN
ajlm-15	39	26	.	.	PUNCT
ajlm-15	40	1	however	however	ADV
ajlm-15	40	2	,	,	PUNCT
ajlm-15	40	3	when	when	SCONJ
ajlm-15	40	4	performing	perform	VERB
ajlm-15	40	5	the	the	DET
ajlm-15	40	6	non	non	ADJ
ajlm-15	40	7	-	-	ADJ
ajlm-15	40	8	validated	validated	ADJ
ajlm-15	40	9	mk016	mk016	NOUN
ajlm-15	40	10	assay	assay	NOUN
ajlm-15	40	11	on	on	ADP
ajlm-15	40	12	the	the	DET
ajlm-15	40	13	phd	phd	NOUN
ajlm-15	40	14	™	™	ADJ
ajlm-15	40	15	system	system	NOUN
ajlm-15	40	16	,	,	PUNCT
ajlm-15	40	17	the	the	DET
ajlm-15	40	18	calibrators	calibrator	NOUN
ajlm-15	40	19	did	do	AUX
ajlm-15	40	20	not	not	PART
ajlm-15	40	21	produce	produce	VERB
ajlm-15	40	22	the	the	DET
ajlm-15	40	23	required	require	VERB
ajlm-15	40	24	od	od	PROPN
ajlm-15	40	25	values	value	NOUN
ajlm-15	40	26	recommended	recommend	VERB
ajlm-15	40	27	by	by	ADP
ajlm-15	40	28	the	the	DET
ajlm-15	40	29	quality	quality	NOUN
ajlm-15	40	30	control	control	NOUN
ajlm-15	40	31	sheet	sheet	NOUN
ajlm-15	40	32	included	include	VERB
ajlm-15	40	33	in	in	ADP
ajlm-15	40	34	the	the	DET
ajlm-15	40	35	kit	kit	NOUN
ajlm-15	40	36	(	(	PUNCT
ajlm-15	40	37	figure	figure	NOUN
ajlm-15	40	38	1	1	NUM
ajlm-15	40	39	)	)	PUNCT
ajlm-15	40	40	.	.	PUNCT
ajlm-15	41	1	figure	figure	VERB
ajlm-15	41	2	1	1	NUM
ajlm-15	41	3	:	:	PUNCT
ajlm-15	41	4	vacczyme	vacczyme	VERB
ajlm-15	41	5	™	™	VERB
ajlm-15	41	6	anti	anti	ADJ
ajlm-15	41	7	-	-	ADJ
ajlm-15	41	8	hib	hib	PRON
ajlm-15	41	9	enzyme	enzyme	NOUN
ajlm-15	41	10	-	-	PUNCT
ajlm-15	41	11	linked	link	VERB
ajlm-15	41	12	immunosorbent	immunosorbent	NOUN
ajlm-15	41	13	assays	assay	NOUN
ajlm-15	41	14	calibration	calibration	NOUN
ajlm-15	41	15	curve	curve	NOUN
ajlm-15	41	16	as	as	SCONJ
ajlm-15	41	17	provided	provide	VERB
ajlm-15	41	18	on	on	ADP
ajlm-15	41	19	the	the	DET
ajlm-15	41	20	quality	quality	NOUN
ajlm-15	41	21	control	control	NOUN
ajlm-15	41	22	sheet	sheet	NOUN
ajlm-15	41	23	.	.	PUNCT
ajlm-15	42	1	the	the	DET
ajlm-15	42	2	highest	high	ADJ
ajlm-15	42	3	calibrator	calibrator	NOUN
ajlm-15	42	4	reached	reach	VERB
ajlm-15	42	5	an	an	DET
ajlm-15	42	6	od	od	NOUN
ajlm-15	42	7	of	of	ADP
ajlm-15	42	8	only	only	ADV
ajlm-15	42	9	1.417	1.417	NUM
ajlm-15	42	10	±	±	NOUN
ajlm-15	42	11	 	 	SPACE
ajlm-15	42	12	0.245	0.245	NUM
ajlm-15	42	13	(	(	PUNCT
ajlm-15	42	14	range	range	NOUN
ajlm-15	42	15	1.252–1.813	1.252–1.813	NUM
ajlm-15	42	16	)	)	PUNCT
ajlm-15	42	17	instead	instead	ADV
ajlm-15	42	18	of	of	ADP
ajlm-15	42	19	2.500	2.500	NUM
ajlm-15	42	20	as	as	ADV
ajlm-15	42	21	specified	specify	VERB
ajlm-15	42	22	on	on	ADP
ajlm-15	42	23	the	the	DET
ajlm-15	42	24	quality	quality	NOUN
ajlm-15	42	25	control	control	NOUN
ajlm-15	42	26	certificate	certificate	NOUN
ajlm-15	42	27	.	.	PUNCT
ajlm-15	43	1	although	although	SCONJ
ajlm-15	43	2	the	the	DET
ajlm-15	43	3	lower	low	ADJ
ajlm-15	43	4	calibrators	calibrator	NOUN
ajlm-15	43	5	were	be	AUX
ajlm-15	43	6	associated	associate	VERB
ajlm-15	43	7	with	with	ADP
ajlm-15	43	8	smaller	small	ADJ
ajlm-15	43	9	margins	margin	NOUN
ajlm-15	43	10	of	of	ADP
ajlm-15	43	11	error	error	NOUN
ajlm-15	43	12	,	,	PUNCT
ajlm-15	43	13	they	they	PRON
ajlm-15	43	14	showed	show	VERB
ajlm-15	43	15	a	a	DET
ajlm-15	43	16	similar	similar	ADJ
ajlm-15	43	17	trend	trend	NOUN
ajlm-15	43	18	.	.	PUNCT
ajlm-15	44	1	all	all	DET
ajlm-15	44	2	results	result	NOUN
ajlm-15	44	3	calculated	calculate	VERB
ajlm-15	44	4	from	from	ADP
ajlm-15	44	5	this	this	DET
ajlm-15	44	6	calibration	calibration	NOUN
ajlm-15	44	7	curve	curve	NOUN
ajlm-15	44	8	were	be	AUX
ajlm-15	44	9	therefore	therefore	ADV
ajlm-15	44	10	too	too	ADV
ajlm-15	44	11	low	low	ADJ
ajlm-15	44	12	and	and	CCONJ
ajlm-15	44	13	hence	hence	ADV
ajlm-15	44	14	invalid.comparison	invalid.comparison	NOUN
ajlm-15	44	15	of	of	ADP
ajlm-15	44	16	the	the	DET
ajlm-15	44	17	individual	individual	ADJ
ajlm-15	44	18	steps	step	NOUN
ajlm-15	44	19	of	of	ADP
ajlm-15	44	20	the	the	DET
ajlm-15	44	21	automated	automate	VERB
ajlm-15	44	22	and	and	CCONJ
ajlm-15	44	23	manual	manual	ADJ
ajlm-15	44	24	methods	method	NOUN
ajlm-15	44	25	identified	identify	VERB
ajlm-15	44	26	a	a	DET
ajlm-15	44	27	difference	difference	NOUN
ajlm-15	44	28	of	of	ADP
ajlm-15	44	29	10	10	NUM
ajlm-15	44	30	minutes	minute	NOUN
ajlm-15	44	31	in	in	ADP
ajlm-15	44	32	all	all	DET
ajlm-15	44	33	incubation	incubation	NOUN
ajlm-15	44	34	periods	period	NOUN
ajlm-15	44	35	.	.	PUNCT
ajlm-15	45	1	the	the	DET
ajlm-15	45	2	phd	phd	NOUN
ajlm-15	45	3	™	™	ADJ
ajlm-15	45	4	system	system	NOUN
ajlm-15	45	5	times	time	NOUN
ajlm-15	45	6	incubation	incubation	NOUN
ajlm-15	45	7	for	for	ADP
ajlm-15	45	8	each	each	DET
ajlm-15	45	9	well	well	ADV
ajlm-15	45	10	individually	individually	ADV
ajlm-15	45	11	and	and	CCONJ
ajlm-15	45	12	proceeds	proceed	VERB
ajlm-15	45	13	to	to	ADP
ajlm-15	45	14	the	the	DET
ajlm-15	45	15	next	next	ADJ
ajlm-15	45	16	step	step	NOUN
ajlm-15	45	17	only	only	ADV
ajlm-15	45	18	once	once	SCONJ
ajlm-15	45	19	the	the	DET
ajlm-15	45	20	exact	exact	ADJ
ajlm-15	45	21	incubation	incubation	NOUN
ajlm-15	45	22	time	time	NOUN
ajlm-15	45	23	has	have	AUX
ajlm-15	45	24	been	be	AUX
ajlm-15	45	25	reached	reach	VERB
ajlm-15	45	26	for	for	ADP
ajlm-15	45	27	that	that	DET
ajlm-15	45	28	specific	specific	ADJ
ajlm-15	45	29	well	well	NOUN
ajlm-15	45	30	.	.	PUNCT
ajlm-15	46	1	however	however	ADV
ajlm-15	46	2	,	,	PUNCT
ajlm-15	46	3	timing	timing	NOUN
ajlm-15	46	4	of	of	ADP
ajlm-15	46	5	manual	manual	ADJ
ajlm-15	46	6	assays	assay	NOUN
ajlm-15	46	7	usually	usually	ADV
ajlm-15	46	8	starts	start	VERB
ajlm-15	46	9	only	only	ADV
ajlm-15	46	10	after	after	SCONJ
ajlm-15	46	11	reagents	reagent	NOUN
ajlm-15	46	12	have	have	AUX
ajlm-15	46	13	been	be	AUX
ajlm-15	46	14	added	add	VERB
ajlm-15	46	15	to	to	ADP
ajlm-15	46	16	the	the	DET
ajlm-15	46	17	last	last	ADJ
ajlm-15	46	18	well	well	NOUN
ajlm-15	46	19	of	of	ADP
ajlm-15	46	20	the	the	DET
ajlm-15	46	21	plate	plate	NOUN
ajlm-15	46	22	;	;	PUNCT
ajlm-15	46	23	i.e.	i.e.	X
ajlm-15	46	24	well	well	ADV
ajlm-15	46	25	incubation	incubation	NOUN
ajlm-15	46	26	is	be	AUX
ajlm-15	46	27	timed	time	VERB
ajlm-15	46	28	rather	rather	ADV
ajlm-15	46	29	than	than	ADP
ajlm-15	46	30	plate	plate	NOUN
ajlm-15	46	31	incubation	incubation	NOUN
ajlm-15	46	32	.	.	PUNCT
ajlm-15	47	1	in	in	ADP
ajlm-15	47	2	an	an	DET
ajlm-15	47	3	effort	effort	NOUN
ajlm-15	47	4	to	to	PART
ajlm-15	47	5	address	address	VERB
ajlm-15	47	6	this	this	DET
ajlm-15	47	7	difference	difference	NOUN
ajlm-15	47	8	,	,	PUNCT
ajlm-15	47	9	all	all	DET
ajlm-15	47	10	incubation	incubation	NOUN
ajlm-15	47	11	steps	step	NOUN
ajlm-15	47	12	were	be	AUX
ajlm-15	47	13	increased	increase	VERB
ajlm-15	47	14	from	from	ADP
ajlm-15	47	15	30	30	NUM
ajlm-15	47	16	minutes	minute	NOUN
ajlm-15	47	17	to	to	ADP
ajlm-15	47	18	40	40	NUM
ajlm-15	47	19	minutes	minute	NOUN
ajlm-15	47	20	on	on	ADP
ajlm-15	47	21	the	the	DET
ajlm-15	47	22	phd	phd	NOUN
ajlm-15	47	23	™	™	ADJ
ajlm-15	47	24	system	system	NOUN
ajlm-15	47	25	.	.	PUNCT
ajlm-15	48	1	the	the	DET
ajlm-15	48	2	duration	duration	NOUN
ajlm-15	48	3	of	of	ADP
ajlm-15	48	4	the	the	DET
ajlm-15	48	5	washing	washing	NOUN
ajlm-15	48	6	steps	step	NOUN
ajlm-15	48	7	of	of	ADP
ajlm-15	48	8	the	the	DET
ajlm-15	48	9	two	two	NUM
ajlm-15	48	10	methods	method	NOUN
ajlm-15	48	11	was	be	AUX
ajlm-15	48	12	very	very	ADV
ajlm-15	48	13	similar	similar	ADJ
ajlm-15	48	14	and	and	CCONJ
ajlm-15	48	15	therefore	therefore	ADV
ajlm-15	48	16	not	not	PART
ajlm-15	48	17	regarded	regard	VERB
ajlm-15	48	18	as	as	ADP
ajlm-15	48	19	contributing	contribute	VERB
ajlm-15	48	20	to	to	ADP
ajlm-15	48	21	the	the	DET
ajlm-15	48	22	discrepant	discrepant	NOUN
ajlm-15	48	23	results	result	NOUN
ajlm-15	48	24	.	.	PUNCT
ajlm-15	49	1	the	the	DET
ajlm-15	49	2	duration	duration	NOUN
ajlm-15	49	3	of	of	ADP
ajlm-15	49	4	the	the	DET
ajlm-15	49	5	washing	washing	NOUN
ajlm-15	49	6	steps	step	NOUN
ajlm-15	49	7	was	be	AUX
ajlm-15	49	8	therefore	therefore	ADV
ajlm-15	49	9	left	leave	VERB
ajlm-15	49	10	unchanged	unchanged	ADJ
ajlm-15	49	11	.	.	PUNCT
ajlm-15	50	1	a	a	DET
ajlm-15	50	2	total	total	NOUN
ajlm-15	50	3	of	of	ADP
ajlm-15	50	4	16	16	NUM
ajlm-15	50	5	calibration	calibration	NOUN
ajlm-15	50	6	curves	curve	NOUN
ajlm-15	50	7	were	be	AUX
ajlm-15	50	8	subsequently	subsequently	ADV
ajlm-15	50	9	generated	generate	VERB
ajlm-15	50	10	from	from	ADP
ajlm-15	50	11	the	the	DET
ajlm-15	50	12	phd	phd	NOUN
ajlm-15	50	13	™	™	ADJ
ajlm-15	50	14	system	system	NOUN
ajlm-15	50	15	to	to	PART
ajlm-15	50	16	validate	validate	VERB
ajlm-15	50	17	the	the	DET
ajlm-15	50	18	adjusted	adjust	VERB
ajlm-15	50	19	protocol	protocol	NOUN
ajlm-15	50	20	.	.	PUNCT
ajlm-15	51	1	all	all	DET
ajlm-15	51	2	the	the	DET
ajlm-15	51	3	od	od	PROPN
ajlm-15	51	4	values	value	NOUN
ajlm-15	51	5	obtained	obtain	VERB
ajlm-15	51	6	produced	produce	VERB
ajlm-15	51	7	acceptable	acceptable	ADJ
ajlm-15	51	8	calibration	calibration	NOUN
ajlm-15	51	9	curves	curve	NOUN
ajlm-15	51	10	(	(	PUNCT
ajlm-15	51	11	figure	figure	NOUN
ajlm-15	51	12	2	2	NUM
ajlm-15	51	13	)	)	PUNCT
ajlm-15	51	14	and	and	CCONJ
ajlm-15	51	15	the	the	DET
ajlm-15	51	16	mean	mean	NOUN
ajlm-15	51	17	od	od	NUM
ajlm-15	51	18	value	value	NOUN
ajlm-15	51	19	for	for	ADP
ajlm-15	51	20	the	the	DET
ajlm-15	51	21	highest	high	ADJ
ajlm-15	51	22	calibrator	calibrator	NOUN
ajlm-15	51	23	reached	reach	VERB
ajlm-15	51	24	2.295	2.295	NUM
ajlm-15	51	25	 	 	SPACE
ajlm-15	51	26	±	±	NOUN
ajlm-15	51	27	 	 	SPACE
ajlm-15	51	28	0.171	0.171	NUM
ajlm-15	51	29	(	(	PUNCT
ajlm-15	51	30	median	median	NOUN
ajlm-15	51	31	=	=	PROPN
ajlm-15	51	32	2.405	2.405	NUM
ajlm-15	51	33	;	;	PUNCT
ajlm-15	51	34	range	range	NOUN
ajlm-15	51	35	=	=	SYM
ajlm-15	51	36	1.934–2.553	1.934–2.553	NUM
ajlm-15	51	37	)	)	PUNCT
ajlm-15	51	38	.	.	PUNCT
ajlm-15	52	1	the	the	DET
ajlm-15	52	2	recommended	recommend	VERB
ajlm-15	52	3	values	value	NOUN
ajlm-15	52	4	for	for	ADP
ajlm-15	52	5	the	the	DET
ajlm-15	52	6	high	high	ADJ
ajlm-15	52	7	and	and	CCONJ
ajlm-15	52	8	low	low	ADJ
ajlm-15	52	9	controls	control	NOUN
ajlm-15	52	10	are	be	AUX
ajlm-15	52	11	2.4	2.4	NUM
ajlm-15	52	12	 	 	SPACE
ajlm-15	52	13	mg	mg	PROPN
ajlm-15	52	14	/	/	SYM
ajlm-15	52	15	l	l	NOUN
ajlm-15	52	16	–	–	PUNCT
ajlm-15	52	17	 	 	SPACE
ajlm-15	52	18	3.6	3.6	NUM
ajlm-15	52	19	 	 	SPACE
ajlm-15	52	20	mg	mg	PROPN
ajlm-15	52	21	/	/	SYM
ajlm-15	52	22	l	l	NOUN
ajlm-15	52	23	and	and	CCONJ
ajlm-15	52	24	<	<	X
ajlm-15	52	25	0.35	0.35	NUM
ajlm-15	52	26	 	 	SPACE
ajlm-15	52	27	mg	mg	PROPN
ajlm-15	52	28	/	/	SYM
ajlm-15	52	29	l	l	NOUN
ajlm-15	52	30	,	,	PUNCT
ajlm-15	52	31	respectively	respectively	ADV
ajlm-15	52	32	.	.	PUNCT
ajlm-15	53	1	the	the	DET
ajlm-15	53	2	high	high	ADJ
ajlm-15	53	3	and	and	CCONJ
ajlm-15	53	4	low	low	ADJ
ajlm-15	53	5	controls	control	NOUN
ajlm-15	53	6	as	as	SCONJ
ajlm-15	53	7	used	use	VERB
ajlm-15	53	8	on	on	ADP
ajlm-15	53	9	the	the	DET
ajlm-15	53	10	phd	phd	NOUN
ajlm-15	53	11	™	™	ADJ
ajlm-15	53	12	system	system	NOUN
ajlm-15	53	13	produced	produce	VERB
ajlm-15	53	14	results	result	NOUN
ajlm-15	53	15	of	of	ADP
ajlm-15	53	16	2.538	2.538	NUM
ajlm-15	53	17	 	 	SPACE
ajlm-15	53	18	±	±	NOUN
ajlm-15	53	19	 	 	SPACE
ajlm-15	53	20	0.094	0.094	NUM
ajlm-15	53	21	 	 	SPACE
ajlm-15	53	22	mg	mg	PROPN
ajlm-15	53	23	/	/	SYM
ajlm-15	53	24	l	l	NOUN
ajlm-15	53	25	and	and	CCONJ
ajlm-15	53	26	<	<	X
ajlm-15	53	27	0.35	0.35	NUM
ajlm-15	53	28	 	 	SPACE
ajlm-15	53	29	mg	mg	PROPN
ajlm-15	53	30	/	/	SYM
ajlm-15	53	31	l	l	NOUN
ajlm-15	53	32	,	,	PUNCT
ajlm-15	53	33	respectively	respectively	ADV
ajlm-15	53	34	.	.	PUNCT
ajlm-15	54	1	figure	figure	NOUN
ajlm-15	54	2	2	2	NUM
ajlm-15	54	3	:	:	PUNCT
ajlm-15	54	4	vacczyme	vacczyme	VERB
ajlm-15	54	5	™	™	VERB
ajlm-15	54	6	anti	anti	ADJ
ajlm-15	54	7	-	-	ADJ
ajlm-15	54	8	hib	hib	PRON
ajlm-15	54	9	enzyme	enzyme	NOUN
ajlm-15	54	10	-	-	PUNCT
ajlm-15	54	11	linked	link	VERB
ajlm-15	54	12	immunosorbent	immunosorbent	NOUN
ajlm-15	54	13	assay	assay	ADJ
ajlm-15	54	14	calibration	calibration	NOUN
ajlm-15	54	15	curves	curve	NOUN
ajlm-15	54	16	before	before	ADV
ajlm-15	54	17	and	and	CCONJ
ajlm-15	54	18	after	after	ADP
ajlm-15	54	19	optimisation	optimisation	NOUN
ajlm-15	54	20	.	.	PUNCT
ajlm-15	55	1	to	to	PART
ajlm-15	55	2	confirm	confirm	VERB
ajlm-15	55	3	our	our	PRON
ajlm-15	55	4	findings	finding	NOUN
ajlm-15	55	5	,	,	PUNCT
ajlm-15	55	6	calibrators	calibrator	NOUN
ajlm-15	55	7	and	and	CCONJ
ajlm-15	55	8	controls	control	NOUN
ajlm-15	55	9	were	be	AUX
ajlm-15	55	10	included	include	VERB
ajlm-15	55	11	in	in	ADP
ajlm-15	55	12	different	different	ADJ
ajlm-15	55	13	positions	position	NOUN
ajlm-15	55	14	and	and	CCONJ
ajlm-15	55	15	orders	order	NOUN
ajlm-15	55	16	on	on	ADP
ajlm-15	55	17	the	the	DET
ajlm-15	55	18	plate	plate	NOUN
ajlm-15	55	19	.	.	PUNCT
ajlm-15	56	1	values	value	NOUN
ajlm-15	56	2	obtained	obtain	VERB
ajlm-15	56	3	for	for	ADP
ajlm-15	56	4	these	these	DET
ajlm-15	56	5	tests	test	NOUN
ajlm-15	56	6	were	be	AUX
ajlm-15	56	7	within	within	ADP
ajlm-15	56	8	5	5	NUM
ajlm-15	56	9	%	%	NOUN
ajlm-15	56	10	of	of	ADP
ajlm-15	56	11	the	the	DET
ajlm-15	56	12	required	require	VERB
ajlm-15	56	13	ranges	range	NOUN
ajlm-15	56	14	,	,	PUNCT
ajlm-15	56	15	which	which	PRON
ajlm-15	56	16	suggested	suggest	VERB
ajlm-15	56	17	that	that	SCONJ
ajlm-15	56	18	the	the	DET
ajlm-15	56	19	amended	amend	VERB
ajlm-15	56	20	protocol	protocol	NOUN
ajlm-15	56	21	had	have	VERB
ajlm-15	56	22	a	a	DET
ajlm-15	56	23	uniform	uniform	ADJ
ajlm-15	56	24	effect	effect	NOUN
ajlm-15	56	25	on	on	ADP
ajlm-15	56	26	all	all	DET
ajlm-15	56	27	individual	individual	ADJ
ajlm-15	56	28	wells	well	NOUN
ajlm-15	56	29	of	of	ADP
ajlm-15	56	30	the	the	DET
ajlm-15	56	31	plate	plate	NOUN
ajlm-15	56	32	(	(	PUNCT
ajlm-15	56	33	data	datum	NOUN
ajlm-15	56	34	not	not	PART
ajlm-15	56	35	shown	show	VERB
ajlm-15	56	36	)	)	PUNCT
ajlm-15	56	37	.	.	PUNCT
ajlm-15	57	1	discussion	discussion	NOUN
ajlm-15	57	2	top	top	PROPN
ajlm-15	57	3	↑	↑	PROPN
ajlm-15	57	4	automated	automate	VERB
ajlm-15	57	5	systems	system	NOUN
ajlm-15	57	6	,	,	PUNCT
ajlm-15	57	7	such	such	ADJ
ajlm-15	57	8	as	as	ADP
ajlm-15	57	9	the	the	DET
ajlm-15	57	10	biorad	biorad	ADJ
ajlm-15	57	11	phd	phd	NOUN
ajlm-15	57	12	™	™	ADJ
ajlm-15	57	13	instrument	instrument	NOUN
ajlm-15	57	14	,	,	PUNCT
ajlm-15	57	15	are	be	AUX
ajlm-15	57	16	extremely	extremely	ADV
ajlm-15	57	17	useful	useful	ADJ
ajlm-15	57	18	and	and	CCONJ
ajlm-15	57	19	accurate	accurate	ADJ
ajlm-15	57	20	in	in	ADP
ajlm-15	57	21	assessing	assess	VERB
ajlm-15	57	22	immune	immune	ADJ
ajlm-15	57	23	responses	response	NOUN
ajlm-15	57	24	to	to	ADP
ajlm-15	57	25	specific	specific	ADJ
ajlm-15	57	26	antigens	antigen	NOUN
ajlm-15	57	27	following	follow	VERB
ajlm-15	57	28	disease	disease	NOUN
ajlm-15	57	29	or	or	CCONJ
ajlm-15	57	30	vaccination	vaccination	NOUN
ajlm-15	57	31	.	.	PUNCT
ajlm-15	58	1	the	the	DET
ajlm-15	58	2	major	major	ADJ
ajlm-15	58	3	advantages	advantage	NOUN
ajlm-15	58	4	of	of	ADP
ajlm-15	58	5	automation	automation	NOUN
ajlm-15	58	6	or	or	CCONJ
ajlm-15	58	7	semi	semi	ADJ
ajlm-15	58	8	-	-	NOUN
ajlm-15	58	9	automation	automation	NOUN
ajlm-15	58	10	include	include	VERB
ajlm-15	58	11	the	the	DET
ajlm-15	58	12	use	use	NOUN
ajlm-15	58	13	of	of	ADP
ajlm-15	58	14	volumes	volume	NOUN
ajlm-15	58	15	as	as	ADV
ajlm-15	58	16	low	low	ADJ
ajlm-15	58	17	as	as	ADP
ajlm-15	58	18	1	1	NUM
ajlm-15	58	19	ml	ml	NOUN
ajlm-15	58	20	,	,	PUNCT
ajlm-15	58	21	increased	increase	VERB
ajlm-15	58	22	accuracy	accuracy	NOUN
ajlm-15	58	23	and	and	CCONJ
ajlm-15	58	24	reproducibility	reproducibility	NOUN
ajlm-15	58	25	of	of	ADP
ajlm-15	58	26	results	result	NOUN
ajlm-15	58	27	,	,	PUNCT
ajlm-15	58	28	better	well	ADJ
ajlm-15	58	29	use	use	NOUN
ajlm-15	58	30	of	of	ADP
ajlm-15	58	31	expensive	expensive	ADJ
ajlm-15	58	32	skilled	skilled	ADJ
ajlm-15	58	33	labour	labour	NOUN
ajlm-15	58	34	,	,	PUNCT
ajlm-15	58	35	faster	fast	ADV
ajlm-15	58	36	overall	overall	ADJ
ajlm-15	58	37	laboratory	laboratory	NOUN
ajlm-15	58	38	turnaround	turnaround	NOUN
ajlm-15	58	39	time	time	NOUN
ajlm-15	58	40	,	,	PUNCT
ajlm-15	58	41	ability	ability	NOUN
ajlm-15	58	42	to	to	PART
ajlm-15	58	43	perform	perform	VERB
ajlm-15	58	44	multiple	multiple	ADJ
ajlm-15	58	45	assays	assay	NOUN
ajlm-15	58	46	simultaneously	simultaneously	ADV
ajlm-15	58	47	and	and	CCONJ
ajlm-15	58	48	cost	cost	NOUN
ajlm-15	58	49	effectiveness	effectiveness	NOUN
ajlm-15	58	50	due	due	ADP
ajlm-15	58	51	to	to	ADP
ajlm-15	58	52	use	use	NOUN
ajlm-15	58	53	of	of	ADP
ajlm-15	58	54	multianalyte	multianalyte	NOUN
ajlm-15	58	55	reagents	reagent	NOUN
ajlm-15	58	56	.	.	PUNCT
ajlm-15	59	1	use	use	NOUN
ajlm-15	59	2	of	of	ADP
ajlm-15	59	3	an	an	DET
ajlm-15	59	4	automated	automate	VERB
ajlm-15	59	5	system	system	NOUN
ajlm-15	59	6	also	also	ADV
ajlm-15	59	7	eliminates	eliminate	VERB
ajlm-15	59	8	pipette	pipette	NOUN
ajlm-15	59	9	volume	volume	NOUN
ajlm-15	59	10	variation	variation	NOUN
ajlm-15	59	11	and	and	CCONJ
ajlm-15	59	12	handling	handle	VERB
ajlm-15	59	13	errors.accurate	errors.accurate	ADJ
ajlm-15	59	14	,	,	PUNCT
ajlm-15	59	15	reproducible	reproducible	ADJ
ajlm-15	59	16	and	and	CCONJ
ajlm-15	59	17	reliable	reliable	ADJ
ajlm-15	59	18	laboratory	laboratory	NOUN
ajlm-15	59	19	results	result	NOUN
ajlm-15	59	20	are	be	AUX
ajlm-15	59	21	crucial	crucial	ADJ
ajlm-15	59	22	for	for	ADP
ajlm-15	59	23	patient	patient	ADJ
ajlm-15	59	24	management	management	NOUN
ajlm-15	59	25	and	and	CCONJ
ajlm-15	59	26	care	care	NOUN
ajlm-15	59	27	,	,	PUNCT
ajlm-15	59	28	such	such	ADJ
ajlm-15	59	29	as	as	ADP
ajlm-15	59	30	initiating	initiate	VERB
ajlm-15	59	31	treatment	treatment	NOUN
ajlm-15	59	32	in	in	ADP
ajlm-15	59	33	patients	patient	NOUN
ajlm-15	59	34	with	with	ADP
ajlm-15	59	35	possible	possible	ADJ
ajlm-15	59	36	immunodeficiency	immunodeficiency	NOUN
ajlm-15	59	37	and	and	CCONJ
ajlm-15	59	38	revaccination	revaccination	NOUN
ajlm-15	59	39	of	of	ADP
ajlm-15	59	40	children	child	NOUN
ajlm-15	59	41	with	with	ADP
ajlm-15	59	42	insufficient	insufficient	ADJ
ajlm-15	59	43	protection	protection	NOUN
ajlm-15	59	44	following	follow	VERB
ajlm-15	59	45	routine	routine	ADJ
ajlm-15	59	46	childhood	childhood	NOUN
ajlm-15	59	47	vaccination	vaccination	NOUN
ajlm-15	59	48	.	.	PUNCT
ajlm-15	60	1	it	it	PRON
ajlm-15	60	2	is	be	AUX
ajlm-15	60	3	furthermore	furthermore	ADV
ajlm-15	60	4	important	important	ADJ
ajlm-15	60	5	for	for	ADP
ajlm-15	60	6	evaluation	evaluation	NOUN
ajlm-15	60	7	of	of	ADP
ajlm-15	60	8	study	study	NOUN
ajlm-15	60	9	cohort	cohort	NOUN
ajlm-15	60	10	results	result	NOUN
ajlm-15	60	11	or	or	CCONJ
ajlm-15	60	12	establishing	establish	VERB
ajlm-15	60	13	reference	reference	NOUN
ajlm-15	60	14	ranges	range	VERB
ajlm-15	60	15	for	for	ADP
ajlm-15	60	16	specific	specific	ADJ
ajlm-15	60	17	populations	population	NOUN
ajlm-15	60	18	or	or	CCONJ
ajlm-15	60	19	age	age	NOUN
ajlm-15	60	20	groups	group	NOUN
ajlm-15	60	21	.	.	PUNCT
ajlm-15	61	1	this	this	DET
ajlm-15	61	2	study	study	NOUN
ajlm-15	61	3	emphasises	emphasise	VERB
ajlm-15	61	4	the	the	DET
ajlm-15	61	5	importance	importance	NOUN
ajlm-15	61	6	of	of	ADP
ajlm-15	61	7	optimisation	optimisation	NOUN
ajlm-15	61	8	and	and	CCONJ
ajlm-15	61	9	validation	validation	NOUN
ajlm-15	61	10	whenever	whenever	SCONJ
ajlm-15	61	11	changing	change	VERB
ajlm-15	61	12	protocols	protocol	NOUN
ajlm-15	61	13	or	or	CCONJ
ajlm-15	61	14	reagents	reagent	NOUN
ajlm-15	61	15	in	in	ADP
ajlm-15	61	16	order	order	NOUN
ajlm-15	61	17	to	to	PART
ajlm-15	61	18	produce	produce	VERB
ajlm-15	61	19	valid	valid	ADJ
ajlm-15	61	20	and	and	CCONJ
ajlm-15	61	21	accurate	accurate	ADJ
ajlm-15	61	22	results	result	NOUN
ajlm-15	61	23	.	.	PUNCT
ajlm-15	62	1	in	in	ADP
ajlm-15	62	2	the	the	DET
ajlm-15	62	3	case	case	NOUN
ajlm-15	62	4	of	of	ADP
ajlm-15	62	5	the	the	DET
ajlm-15	62	6	mk016	mk016	PROPN
ajlm-15	62	7	assay	assay	NOUN
ajlm-15	62	8	it	it	PRON
ajlm-15	62	9	was	be	AUX
ajlm-15	62	10	not	not	PART
ajlm-15	62	11	possible	possible	ADJ
ajlm-15	62	12	to	to	PART
ajlm-15	62	13	transfer	transfer	VERB
ajlm-15	62	14	the	the	DET
ajlm-15	62	15	protocol	protocol	NOUN
ajlm-15	62	16	established	establish	VERB
ajlm-15	62	17	for	for	ADP
ajlm-15	62	18	the	the	DET
ajlm-15	62	19	manual	manual	ADJ
ajlm-15	62	20	method	method	NOUN
ajlm-15	62	21	to	to	ADP
ajlm-15	62	22	the	the	DET
ajlm-15	62	23	automated	automate	VERB
ajlm-15	62	24	system	system	NOUN
ajlm-15	62	25	without	without	ADP
ajlm-15	62	26	modification	modification	NOUN
ajlm-15	62	27	.	.	PUNCT
ajlm-15	63	1	after	after	ADP
ajlm-15	63	2	troubleshooting	troubleshoot	VERB
ajlm-15	63	3	all	all	DET
ajlm-15	63	4	the	the	DET
ajlm-15	63	5	individual	individual	ADJ
ajlm-15	63	6	steps	step	NOUN
ajlm-15	63	7	of	of	ADP
ajlm-15	63	8	both	both	DET
ajlm-15	63	9	methods	method	NOUN
ajlm-15	63	10	,	,	PUNCT
ajlm-15	63	11	a	a	DET
ajlm-15	63	12	methodological	methodological	ADJ
ajlm-15	63	13	difference	difference	NOUN
ajlm-15	63	14	was	be	AUX
ajlm-15	63	15	identified	identify	VERB
ajlm-15	63	16	and	and	CCONJ
ajlm-15	63	17	addressed	address	VERB
ajlm-15	63	18	.	.	PUNCT
ajlm-15	64	1	the	the	DET
ajlm-15	64	2	modified	modify	VERB
ajlm-15	64	3	protocol	protocol	NOUN
ajlm-15	64	4	was	be	AUX
ajlm-15	64	5	scrutinised	scrutinise	VERB
ajlm-15	64	6	by	by	ADP
ajlm-15	64	7	repeated	repeat	VERB
ajlm-15	64	8	measurements	measurement	NOUN
ajlm-15	64	9	of	of	ADP
ajlm-15	64	10	samples	sample	NOUN
ajlm-15	64	11	of	of	ADP
ajlm-15	64	12	known	know	VERB
ajlm-15	64	13	concentration	concentration	NOUN
ajlm-15	64	14	in	in	ADP
ajlm-15	64	15	different	different	ADJ
ajlm-15	64	16	assays	assay	NOUN
ajlm-15	64	17	and	and	CCONJ
ajlm-15	64	18	plate	plate	NOUN
ajlm-15	64	19	positions	position	NOUN
ajlm-15	64	20	before	before	ADP
ajlm-15	64	21	amending	amend	VERB
ajlm-15	64	22	the	the	DET
ajlm-15	64	23	existing	exist	VERB
ajlm-15	64	24	protocol	protocol	NOUN
ajlm-15	64	25	.	.	PUNCT
ajlm-15	65	1	acknowledgements	acknowledgement	NOUN
ajlm-15	65	2	top	top	ADJ
ajlm-15	65	3	↑	↑	NOUN
ajlm-15	65	4	we	we	PRON
ajlm-15	65	5	would	would	AUX
ajlm-15	65	6	like	like	VERB
ajlm-15	65	7	to	to	PART
ajlm-15	65	8	thank	thank	VERB
ajlm-15	65	9	allere	allere	ADV
ajlm-15	65	10	healthcare	healthcare	PROPN
ajlm-15	65	11	(	(	PUNCT
ajlm-15	65	12	pty	pty	PROPN
ajlm-15	65	13	)	)	PUNCT
ajlm-15	65	14	ltd	ltd	PROPN
ajlm-15	65	15	(	(	PUNCT
ajlm-15	65	16	bedfordview	bedfordview	NOUN
ajlm-15	65	17	,	,	PUNCT
ajlm-15	65	18	south	south	PROPN
ajlm-15	65	19	africa	africa	PROPN
ajlm-15	65	20	)	)	PUNCT
ajlm-15	65	21	for	for	ADP
ajlm-15	65	22	donating	donate	VERB
ajlm-15	65	23	test	test	NOUN
ajlm-15	65	24	kits	kit	NOUN
ajlm-15	65	25	for	for	ADP
ajlm-15	65	26	optimisation	optimisation	NOUN
ajlm-15	65	27	.	.	PUNCT
ajlm-15	66	1	competing	compete	VERB
ajlm-15	66	2	interests	interest	NOUN
ajlm-15	66	3	the	the	DET
ajlm-15	66	4	authors	author	NOUN
ajlm-15	66	5	declare	declare	VERB
ajlm-15	66	6	that	that	SCONJ
ajlm-15	66	7	they	they	PRON
ajlm-15	66	8	have	have	VERB
ajlm-15	66	9	no	no	DET
ajlm-15	66	10	financial	financial	ADJ
ajlm-15	66	11	or	or	CCONJ
ajlm-15	66	12	personal	personal	ADJ
ajlm-15	66	13	relationship(s	relationship(s	NOUN
ajlm-15	66	14	)	)	PUNCT
ajlm-15	66	15	which	which	PRON
ajlm-15	66	16	may	may	AUX
ajlm-15	66	17	have	have	AUX
ajlm-15	66	18	inappropriately	inappropriately	ADV
ajlm-15	66	19	influenced	influence	VERB
ajlm-15	66	20	them	they	PRON
ajlm-15	66	21	in	in	ADP
ajlm-15	66	22	writing	write	VERB
ajlm-15	66	23	this	this	DET
ajlm-15	66	24	paper	paper	NOUN
ajlm-15	66	25	.	.	PUNCT
ajlm-15	67	1	authors	author	NOUN
ajlm-15	67	2	’	’	PART
ajlm-15	67	3	contributions	contribution	NOUN
ajlm-15	67	4	c.d.b	c.d.b	NOUN
ajlm-15	67	5	.	.	PROPN
ajlm-15	67	6	was	be	AUX
ajlm-15	67	7	the	the	DET
ajlm-15	67	8	project	project	NOUN
ajlm-15	67	9	leader	leader	NOUN
ajlm-15	67	10	and	and	CCONJ
ajlm-15	67	11	responsible	responsible	ADJ
ajlm-15	67	12	for	for	ADP
ajlm-15	67	13	experimental	experimental	ADJ
ajlm-15	67	14	and	and	CCONJ
ajlm-15	67	15	project	project	NOUN
ajlm-15	67	16	design	design	NOUN
ajlm-15	67	17	,	,	PUNCT
ajlm-15	67	18	as	as	ADV
ajlm-15	67	19	well	well	ADV
ajlm-15	67	20	as	as	ADP
ajlm-15	67	21	performing	perform	VERB
ajlm-15	67	22	the	the	DET
ajlm-15	67	23	experiments	experiment	NOUN
ajlm-15	67	24	and	and	CCONJ
ajlm-15	67	25	writing	write	VERB
ajlm-15	67	26	the	the	DET
ajlm-15	67	27	manuscript	manuscript	NOUN
ajlm-15	67	28	.	.	PUNCT
ajlm-15	68	1	m.e	m.e	PROPN
ajlm-15	68	2	.	.	PROPN
ajlm-15	68	3	was	be	AUX
ajlm-15	68	4	the	the	DET
ajlm-15	68	5	head	head	NOUN
ajlm-15	68	6	of	of	ADP
ajlm-15	68	7	the	the	DET
ajlm-15	68	8	laboratory	laboratory	NOUN
ajlm-15	68	9	where	where	SCONJ
ajlm-15	68	10	the	the	DET
ajlm-15	68	11	experiments	experiment	NOUN
ajlm-15	68	12	were	be	AUX
ajlm-15	68	13	conducted	conduct	VERB
ajlm-15	68	14	and	and	CCONJ
ajlm-15	68	15	w.p	w.p	PROPN
ajlm-15	68	16	.	.	PROPN
ajlm-15	68	17	provided	provide	VERB
ajlm-15	68	18	valuable	valuable	ADJ
ajlm-15	68	19	input	input	NOUN
ajlm-15	68	20	into	into	ADP
ajlm-15	68	21	the	the	DET
ajlm-15	68	22	final	final	ADJ
ajlm-15	68	23	manuscript	manuscript	NOUN
ajlm-15	68	24	.	.	PUNCT
ajlm-15	69	1	references	reference	NOUN
ajlm-15	69	2	top	top	VERB
ajlm-15	69	3	↑	↑	PROPN
ajlm-15	69	4	1	1	NUM
ajlm-15	69	5	.	.	PUNCT
ajlm-15	70	1	engvall	engvall	PROPN
ajlm-15	70	2	e	e	PROPN
ajlm-15	70	3	,	,	PUNCT
ajlm-15	70	4	perlman	perlman	NOUN
ajlm-15	70	5	p.	p.	NOUN
ajlm-15	70	6	enzyme	enzyme	NOUN
ajlm-15	70	7	-	-	PUNCT
ajlm-15	70	8	linked	link	VERB
ajlm-15	70	9	immunosorbent	immunosorbent	NOUN
ajlm-15	70	10	assay	assay	PROPN
ajlm-15	70	11	(	(	PUNCT
ajlm-15	70	12	elisa	elisa	NOUN
ajlm-15	70	13	)	)	PUNCT
ajlm-15	70	14	.	.	PUNCT
ajlm-15	71	1	quantitative	quantitative	ADJ
ajlm-15	71	2	assay	assay	PROPN
ajlm-15	71	3	of	of	ADP
ajlm-15	71	4	immunoglobulin	immunoglobulin	PROPN
ajlm-15	71	5	g.	g.	PROPN
ajlm-15	71	6	immunochemistry	immunochemistry	PROPN
ajlm-15	71	7	.	.	PUNCT
ajlm-15	72	1	1971;8(9):871–874	1971;8(9):871–874	NUM
ajlm-15	72	2	.	.	PUNCT
ajlm-15	73	1	http://dx.doi.org/10.1016/0019-2791(71)90454-x2	http://dx.doi.org/10.1016/0019-2791(71)90454-x2	PROPN
ajlm-15	73	2	.	.	PUNCT
ajlm-15	74	1	van	van	PROPN
ajlm-15	74	2	weemen	weeman	NOUN
ajlm-15	74	3	bk	bk	PROPN
ajlm-15	74	4	,	,	PUNCT
ajlm-15	74	5	schuurs	schuur	VERB
ajlm-15	74	6	ah	ah	INTJ
ajlm-15	74	7	.	.	PUNCT
ajlm-15	75	1	immunoassay	immunoassay	PROPN
ajlm-15	75	2	using	use	VERB
ajlm-15	75	3	antigen	antigen	NOUN
ajlm-15	75	4	-	-	PUNCT
ajlm-15	75	5	enzyme	enzyme	NOUN
ajlm-15	75	6	conjugates	conjugate	NOUN
ajlm-15	75	7	.	.	PUNCT
ajlm-15	76	1	febs	febs	PROPN
ajlm-15	76	2	lett	lett	PROPN
ajlm-15	76	3	.	.	PUNCT
ajlm-15	77	1	1971;15(3):232–236	1971;15(3):232–236	X
ajlm-15	77	2	.	.	PUNCT
ajlm-15	77	3	http://dx.doi.org/10.1016/0014-5793(71)80319-8	http://dx.doi.org/10.1016/0014-5793(71)80319-8	NOUN
ajlm-15	77	4	3	3	X
ajlm-15	77	5	.	.	X
ajlm-15	77	6	lequin	lequin	PROPN
ajlm-15	77	7	r.	r.	PROPN
ajlm-15	77	8	enzyme	enzyme	PROPN
ajlm-15	77	9	immunoassay	immunoassay	NOUN
ajlm-15	77	10	(	(	PUNCT
ajlm-15	77	11	eia)/enzyme	eia)/enzyme	NOUN
ajlm-15	77	12	-	-	PUNCT
ajlm-15	77	13	linked	link	VERB
ajlm-15	77	14	immunosorbent	immunosorbent	NOUN
ajlm-15	77	15	assay	assay	PROPN
ajlm-15	77	16	(	(	PUNCT
ajlm-15	77	17	elisa	elisa	NOUN
ajlm-15	77	18	)	)	PUNCT
ajlm-15	77	19	.	.	PUNCT
ajlm-15	78	1	clin	clin	PROPN
ajlm-15	78	2	chem	chem	PROPN
ajlm-15	78	3	.	.	PUNCT
ajlm-15	79	1	2005;51(12):2415–2418	2005;51(12):2415–2418	NUM
ajlm-15	79	2	.	.	PUNCT
ajlm-15	80	1	http://dx.doi.org/10.1373/clinchem.2005.051532	http://dx.doi.org/10.1373/clinchem.2005.051532	NOUN
ajlm-15	80	2	4	4	NUM
ajlm-15	80	3	.	.	PUNCT
ajlm-15	81	1	yalow	yalow	NOUN
ajlm-15	81	2	r	r	NOUN
ajlm-15	81	3	,	,	PUNCT
ajlm-15	81	4	berson	berson	PROPN
ajlm-15	81	5	s.	s.	PROPN
ajlm-15	81	6	immunoassay	immunoassay	PROPN
ajlm-15	81	7	of	of	ADP
ajlm-15	81	8	endogenous	endogenous	ADJ
ajlm-15	81	9	plasma	plasma	NOUN
ajlm-15	81	10	insulin	insulin	NOUN
ajlm-15	81	11	in	in	ADP
ajlm-15	81	12	man	man	NOUN
ajlm-15	81	13	.	.	PUNCT
ajlm-15	82	1	j	j	PROPN
ajlm-15	82	2	clin	clin	PROPN
ajlm-15	82	3	invest	invest	VERB
ajlm-15	82	4	1960;39(7):1157–1175	1960;39(7):1157–1175	NUM
ajlm-15	82	5	.	.	PUNCT
ajlm-15	83	1	http://dx.doi.org/10.1172/jci104130	http://dx.doi.org/10.1172/jci104130	ADV
ajlm-15	83	2	5	5	X
ajlm-15	83	3	.	.	PUNCT
ajlm-15	83	4	gosling	gosling	NOUN
ajlm-15	83	5	jp	jp	PROPN
ajlm-15	83	6	.	.	PUNCT
ajlm-15	84	1	a	a	DET
ajlm-15	84	2	decade	decade	NOUN
ajlm-15	84	3	of	of	ADP
ajlm-15	84	4	development	development	NOUN
ajlm-15	84	5	in	in	ADP
ajlm-15	84	6	immunoassay	immunoassay	PROPN
ajlm-15	84	7	methodology	methodology	PROPN
ajlm-15	84	8	.	.	PUNCT
ajlm-15	85	1	clin	clin	PROPN
ajlm-15	85	2	chem	chem	PROPN
ajlm-15	85	3	.	.	PUNCT
ajlm-15	86	1	1990;36(8):1408–1427	1990;36(8):1408–1427	NUM
ajlm-15	86	2	.	.	PUNCT
ajlm-15	87	1	6	6	NUM
ajlm-15	87	2	.	.	PUNCT
ajlm-15	87	3	engvall	engvall	PROPN
ajlm-15	87	4	e.	e.	PROPN
ajlm-15	87	5	the	the	DET
ajlm-15	87	6	elisa	elisa	PROPN
ajlm-15	87	7	,	,	PUNCT
ajlm-15	87	8	enzyme	enzyme	NOUN
ajlm-15	87	9	-	-	PUNCT
ajlm-15	87	10	linked	link	VERB
ajlm-15	87	11	immunosorbent	immunosorbent	NOUN
ajlm-15	87	12	assay	assay	PROPN
ajlm-15	87	13	.	.	PUNCT
ajlm-15	88	1	clin	clin	PROPN
ajlm-15	88	2	chem	chem	PROPN
ajlm-15	88	3	.	.	PUNCT
ajlm-15	89	1	2010;56(2):319–320	2010;56(2):319–320	X
ajlm-15	89	2	.	.	PUNCT
ajlm-15	89	3	http://dx.doi.org/10.1373/clinchem.2009.127803	http://dx.doi.org/10.1373/clinchem.2009.127803	PROPN
ajlm-15	89	4	7	7	NUM
ajlm-15	89	5	.	.	PUNCT
ajlm-15	89	6	miles	mile	NOUN
ajlm-15	89	7	lem	lem	PROPN
ajlm-15	89	8	,	,	PUNCT
ajlm-15	89	9	hales	hales	PROPN
ajlm-15	89	10	cn	cn	PROPN
ajlm-15	89	11	.	.	PROPN
ajlm-15	89	12	labelled	label	VERB
ajlm-15	89	13	antibodies	antibody	NOUN
ajlm-15	89	14	and	and	CCONJ
ajlm-15	89	15	immunological	immunological	ADJ
ajlm-15	89	16	assay	assay	NOUN
ajlm-15	89	17	systems	system	NOUN
ajlm-15	89	18	.	.	PUNCT
ajlm-15	90	1	nature	nature	NOUN
ajlm-15	90	2	1968;219:186–189	1968;219:186–189	NUM
ajlm-15	90	3	.	.	PUNCT
ajlm-15	91	1	http://dx.doi.org/10.1038/219186a0	http://dx.doi.org/10.1038/219186a0	X
ajlm-15	92	1	8	8	X
ajlm-15	92	2	.	.	PUNCT
ajlm-15	92	3	engvall	engvall	PROPN
ajlm-15	92	4	e	e	PROPN
ajlm-15	92	5	,	,	PUNCT
ajlm-15	92	6	jonsson	jonsson	PROPN
ajlm-15	92	7	k	k	PROPN
ajlm-15	92	8	,	,	PUNCT
ajlm-15	92	9	perlmann	perlmann	NOUN
ajlm-15	92	10	p.	p.	NOUN
ajlm-15	92	11	enzyme	enzyme	NOUN
ajlm-15	92	12	-	-	PUNCT
ajlm-15	92	13	linked	link	VERB
ajlm-15	92	14	immunosorbent	immunosorbent	NOUN
ajlm-15	92	15	assay	assay	PROPN
ajlm-15	92	16	,	,	PUNCT
ajlm-15	92	17	elisa	elisa	PROPN
ajlm-15	92	18	.	.	PUNCT
ajlm-15	92	19	ii	ii	PROPN
ajlm-15	92	20	.	.	PUNCT
ajlm-15	93	1	quantitative	quantitative	ADJ
ajlm-15	93	2	assay	assay	NOUN
ajlm-15	93	3	of	of	ADP
ajlm-15	93	4	protein	protein	NOUN
ajlm-15	93	5	antigen	antigen	ADV
ajlm-15	93	6	,	,	PUNCT
ajlm-15	93	7	immunoglobulin	immunoglobulin	NOUN
ajlm-15	93	8	g	g	NOUN
ajlm-15	93	9	,	,	PUNCT
ajlm-15	93	10	by	by	ADP
ajlm-15	93	11	means	mean	NOUN
ajlm-15	93	12	of	of	ADP
ajlm-15	93	13	enzyme	enzyme	NOUN
ajlm-15	93	14	-	-	PUNCT
ajlm-15	93	15	labeled	label	VERB
ajlm-15	93	16	antigen	antigen	NOUN
ajlm-15	93	17	and	and	CCONJ
ajlm-15	93	18	antibody	antibody	NOUN
ajlm-15	93	19	-	-	PUNCT
ajlm-15	93	20	coated	coat	VERB
ajlm-15	93	21	tubes	tube	NOUN
ajlm-15	93	22	.	.	PUNCT
ajlm-15	94	1	biochem	biochem	PROPN
ajlm-15	94	2	biophys	biophys	PROPN
ajlm-15	94	3	acta	acta	PROPN
ajlm-15	94	4	1971;251:427–434	1971;251:427–434	NUM
ajlm-15	94	5	.	.	PUNCT
ajlm-15	95	1	http://dx.doi.org/10.1016/0005-2795(71)90132-2	http://dx.doi.org/10.1016/0005-2795(71)90132-2	X
ajlm-15	95	2	9	9	X
ajlm-15	95	3	.	.	X
ajlm-15	95	4	package	package	NOUN
ajlm-15	95	5	insert	insert	PROPN
ajlm-15	95	6	,	,	PUNCT
ajlm-15	95	7	24	24	NUM
ajlm-15	95	8	may	may	PROPN
ajlm-15	95	9	2006	2006	NUM
ajlm-15	95	10	.	.	PUNCT
ajlm-15	96	1	vacczyme	vacczyme	NOUN
ajlm-15	96	2	™	™	VERB
ajlm-15	96	3	human	human	ADJ
ajlm-15	96	4	anti	anti	ADJ
ajlm-15	96	5	haemophilus	haemophilus	NOUN
ajlm-15	96	6	influenzae	influenzae	X
ajlm-15	96	7	type	type	PROPN
ajlm-15	96	8	b	b	PROPN
ajlm-15	96	9	(	(	PUNCT
ajlm-15	96	10	hib	hib	NOUN
ajlm-15	96	11	)	)	PUNCT
ajlm-15	96	12	enzyme	enzyme	NOUN
ajlm-15	96	13	immunoassay	immunoassay	ADJ
ajlm-15	96	14	kit	kit	NOUN
ajlm-15	96	15	mk016	mk016	PROPN
ajlm-15	96	16	.	.	PUNCT
ajlm-15	97	1	the	the	DET
ajlm-15	97	2	binding	bind	VERB
ajlm-15	97	3	site	site	NOUN
ajlm-15	97	4	,	,	PUNCT
ajlm-15	97	5	birmingham	birmingham	PROPN
ajlm-15	97	6	,	,	PUNCT
ajlm-15	97	7	uk	uk	PROPN
ajlm-15	97	8	.	.	PUNCT