id	sid	tid	token	lemma	pos
ajlm-269	1	1	abstract	abstract	ADJ
ajlm-269	1	2	introduction	introduction	NOUN
ajlm-269	1	3	research	research	NOUN
ajlm-269	1	4	method	method	NOUN
ajlm-269	1	5	and	and	CCONJ
ajlm-269	1	6	design	design	NOUN
ajlm-269	1	7	results	result	NOUN
ajlm-269	1	8	discussion	discussion	NOUN
ajlm-269	1	9	conclusion	conclusion	NOUN
ajlm-269	1	10	acknowledgements	acknowledgement	NOUN
ajlm-269	1	11	references	reference	NOUN
ajlm-269	1	12	about	about	ADP
ajlm-269	1	13	the	the	DET
ajlm-269	1	14	author(s	author(s	NOUN
ajlm-269	1	15	)	)	PUNCT
ajlm-269	1	16	anneta	anneta	PROPN
ajlm-269	1	17	naidoo	naidoo	PROPN
ajlm-269	1	18	department	department	PROPN
ajlm-269	1	19	of	of	ADP
ajlm-269	1	20	virology	virology	NOUN
ajlm-269	1	21	,	,	PUNCT
ajlm-269	1	22	nelson	nelson	PROPN
ajlm-269	1	23	r	r	PROPN
ajlm-269	1	24	mandela	mandela	PROPN
ajlm-269	1	25	school	school	NOUN
ajlm-269	1	26	of	of	ADP
ajlm-269	1	27	medicine	medicine	NOUN
ajlm-269	1	28	,	,	PUNCT
ajlm-269	1	29	school	school	NOUN
ajlm-269	1	30	of	of	ADP
ajlm-269	1	31	laboratory	laboratory	NOUN
ajlm-269	1	32	medicine	medicine	NOUN
ajlm-269	1	33	and	and	CCONJ
ajlm-269	1	34	medical	medical	ADJ
ajlm-269	1	35	sciences	science	NOUN
ajlm-269	1	36	,	,	PUNCT
ajlm-269	1	37	university	university	NOUN
ajlm-269	1	38	of	of	ADP
ajlm-269	1	39	kwazulu	kwazulu	PROPN
ajlm-269	1	40	-	-	PUNCT
ajlm-269	1	41	natal	natal	ADJ
ajlm-269	1	42	,	,	PUNCT
ajlm-269	1	43	durban	durban	PROPN
ajlm-269	1	44	,	,	PUNCT
ajlm-269	1	45	kwazulu	kwazulu	NOUN
ajlm-269	1	46	-	-	PUNCT
ajlm-269	1	47	natal	natal	ADJ
ajlm-269	1	48	,	,	PUNCT
ajlm-269	1	49	south	south	PROPN
ajlm-269	1	50	africa	africa	PROPN
ajlm-269	1	51	national	national	PROPN
ajlm-269	1	52	health	health	PROPN
ajlm-269	1	53	laboratory	laboratory	PROPN
ajlm-269	1	54	services	service	NOUN
ajlm-269	1	55	,	,	PUNCT
ajlm-269	1	56	inkosi	inkosi	PROPN
ajlm-269	1	57	albert	albert	PROPN
ajlm-269	1	58	luthuli	luthuli	PROPN
ajlm-269	1	59	central	central	PROPN
ajlm-269	1	60	hospital	hospital	NOUN
ajlm-269	1	61	,	,	PUNCT
ajlm-269	1	62	durban	durban	PROPN
ajlm-269	1	63	,	,	PUNCT
ajlm-269	1	64	kwazulu	kwazulu	NOUN
ajlm-269	1	65	-	-	PUNCT
ajlm-269	1	66	natal	natal	ADJ
ajlm-269	1	67	,	,	PUNCT
ajlm-269	1	68	south	south	PROPN
ajlm-269	1	69	africa	africa	PROPN
ajlm-269	1	70	raveen	raveen	ADJ
ajlm-269	1	71	parboosing	parboose	VERB
ajlm-269	1	72	department	department	NOUN
ajlm-269	1	73	of	of	ADP
ajlm-269	1	74	virology	virology	NOUN
ajlm-269	1	75	,	,	PUNCT
ajlm-269	1	76	nelson	nelson	PROPN
ajlm-269	1	77	r	r	PROPN
ajlm-269	1	78	mandela	mandela	PROPN
ajlm-269	1	79	school	school	NOUN
ajlm-269	1	80	of	of	ADP
ajlm-269	1	81	medicine	medicine	NOUN
ajlm-269	1	82	,	,	PUNCT
ajlm-269	1	83	school	school	NOUN
ajlm-269	1	84	of	of	ADP
ajlm-269	1	85	laboratory	laboratory	NOUN
ajlm-269	1	86	medicine	medicine	NOUN
ajlm-269	1	87	and	and	CCONJ
ajlm-269	1	88	medical	medical	ADJ
ajlm-269	1	89	sciences	science	NOUN
ajlm-269	1	90	,	,	PUNCT
ajlm-269	1	91	university	university	NOUN
ajlm-269	1	92	of	of	ADP
ajlm-269	1	93	kwazulu	kwazulu	PROPN
ajlm-269	1	94	-	-	PUNCT
ajlm-269	1	95	natal	natal	ADJ
ajlm-269	1	96	,	,	PUNCT
ajlm-269	1	97	durban	durban	PROPN
ajlm-269	1	98	,	,	PUNCT
ajlm-269	1	99	kwazulu	kwazulu	NOUN
ajlm-269	1	100	-	-	PUNCT
ajlm-269	1	101	natal	natal	ADJ
ajlm-269	1	102	,	,	PUNCT
ajlm-269	1	103	south	south	PROPN
ajlm-269	1	104	africa	africa	PROPN
ajlm-269	1	105	national	national	PROPN
ajlm-269	1	106	health	health	PROPN
ajlm-269	1	107	laboratory	laboratory	PROPN
ajlm-269	1	108	services	service	NOUN
ajlm-269	1	109	,	,	PUNCT
ajlm-269	1	110	inkosi	inkosi	PROPN
ajlm-269	1	111	albert	albert	PROPN
ajlm-269	1	112	luthuli	luthuli	PROPN
ajlm-269	1	113	central	central	PROPN
ajlm-269	1	114	hospital	hospital	NOUN
ajlm-269	1	115	,	,	PUNCT
ajlm-269	1	116	durban	durban	PROPN
ajlm-269	1	117	,	,	PUNCT
ajlm-269	1	118	kwazulu	kwazulu	NOUN
ajlm-269	1	119	-	-	PUNCT
ajlm-269	1	120	natal	natal	ADJ
ajlm-269	1	121	,	,	PUNCT
ajlm-269	1	122	south	south	PROPN
ajlm-269	1	123	africa	africa	PROPN
ajlm-269	1	124	pravi	pravi	PROPN
ajlm-269	1	125	moodley	moodley	PROPN
ajlm-269	1	126	department	department	PROPN
ajlm-269	1	127	of	of	ADP
ajlm-269	1	128	virology	virology	NOUN
ajlm-269	1	129	,	,	PUNCT
ajlm-269	1	130	nelson	nelson	PROPN
ajlm-269	1	131	r	r	PROPN
ajlm-269	1	132	mandela	mandela	PROPN
ajlm-269	1	133	school	school	NOUN
ajlm-269	1	134	of	of	ADP
ajlm-269	1	135	medicine	medicine	NOUN
ajlm-269	1	136	,	,	PUNCT
ajlm-269	1	137	school	school	NOUN
ajlm-269	1	138	of	of	ADP
ajlm-269	1	139	laboratory	laboratory	NOUN
ajlm-269	1	140	medicine	medicine	NOUN
ajlm-269	1	141	and	and	CCONJ
ajlm-269	1	142	medical	medical	ADJ
ajlm-269	1	143	sciences	science	NOUN
ajlm-269	1	144	,	,	PUNCT
ajlm-269	1	145	university	university	NOUN
ajlm-269	1	146	of	of	ADP
ajlm-269	1	147	kwazulu	kwazulu	PROPN
ajlm-269	1	148	-	-	PUNCT
ajlm-269	1	149	natal	natal	ADJ
ajlm-269	1	150	,	,	PUNCT
ajlm-269	1	151	durban	durban	PROPN
ajlm-269	1	152	,	,	PUNCT
ajlm-269	1	153	kwazulu	kwazulu	NOUN
ajlm-269	1	154	-	-	PUNCT
ajlm-269	1	155	natal	natal	ADJ
ajlm-269	1	156	,	,	PUNCT
ajlm-269	1	157	south	south	PROPN
ajlm-269	1	158	africa	africa	PROPN
ajlm-269	1	159	national	national	PROPN
ajlm-269	1	160	health	health	PROPN
ajlm-269	1	161	laboratory	laboratory	PROPN
ajlm-269	1	162	services	service	NOUN
ajlm-269	1	163	,	,	PUNCT
ajlm-269	1	164	inkosi	inkosi	PROPN
ajlm-269	1	165	albert	albert	PROPN
ajlm-269	1	166	luthuli	luthuli	PROPN
ajlm-269	1	167	central	central	PROPN
ajlm-269	1	168	hospital	hospital	NOUN
ajlm-269	1	169	,	,	PUNCT
ajlm-269	1	170	durban	durban	PROPN
ajlm-269	1	171	,	,	PUNCT
ajlm-269	1	172	kwazulu	kwazulu	NOUN
ajlm-269	1	173	-	-	PUNCT
ajlm-269	1	174	natal	natal	ADJ
ajlm-269	1	175	,	,	PUNCT
ajlm-269	1	176	south	south	PROPN
ajlm-269	1	177	africa	africa	PROPN
ajlm-269	1	178	citation	citation	PROPN
ajlm-269	1	179	naidoo	naidoo	PROPN
ajlm-269	1	180	a	a	PROPN
ajlm-269	1	181	,	,	PUNCT
ajlm-269	1	182	parboosing	parboose	VERB
ajlm-269	1	183	r	r	NOUN
ajlm-269	1	184	,	,	PUNCT
ajlm-269	1	185	moodley	moodley	NOUN
ajlm-269	1	186	p.	p.	NOUN
ajlm-269	1	187	real	real	ADJ
ajlm-269	1	188	-	-	PUNCT
ajlm-269	1	189	time	time	NOUN
ajlm-269	1	190	polymerase	polymerase	NOUN
ajlm-269	1	191	chain	chain	NOUN
ajlm-269	1	192	reaction	reaction	NOUN
ajlm-269	1	193	optimised	optimise	VERB
ajlm-269	1	194	for	for	ADP
ajlm-269	1	195	hepatitis	hepatitis	PROPN
ajlm-269	1	196	c	c	PROPN
ajlm-269	1	197	virus	virus	NOUN
ajlm-269	1	198	detection	detection	NOUN
ajlm-269	1	199	in	in	ADP
ajlm-269	1	200	dried	dry	VERB
ajlm-269	1	201	blood	blood	NOUN
ajlm-269	1	202	spots	spot	NOUN
ajlm-269	1	203	from	from	ADP
ajlm-269	1	204	hiv	hiv	NOUN
ajlm-269	1	205	-	-	PUNCT
ajlm-269	1	206	exposed	expose	VERB
ajlm-269	1	207	infants	infant	NOUN
ajlm-269	1	208	,	,	PUNCT
ajlm-269	1	209	kwazulu	kwazulu	NOUN
ajlm-269	1	210	-	-	PUNCT
ajlm-269	1	211	natal	natal	ADJ
ajlm-269	1	212	,	,	PUNCT
ajlm-269	1	213	south	south	PROPN
ajlm-269	1	214	africa	africa	PROPN
ajlm-269	1	215	.	.	PUNCT
ajlm-269	2	1	afr	afr	PROPN
ajlm-269	2	2	j	j	PROPN
ajlm-269	2	3	lab	lab	PROPN
ajlm-269	2	4	med	med	PROPN
ajlm-269	2	5	.	.	PROPN
ajlm-269	2	6	2016;5(1	2016;5(1	NUM
ajlm-269	2	7	)	)	PUNCT
ajlm-269	2	8	,	,	PUNCT
ajlm-269	2	9	art	art	NOUN
ajlm-269	2	10	.	.	PUNCT
ajlm-269	3	1	#	#	SYM
ajlm-269	3	2	269	269	NUM
ajlm-269	3	3	,	,	PUNCT
ajlm-269	3	4	6	6	NUM
ajlm-269	3	5	pages	page	NOUN
ajlm-269	3	6	.	.	PUNCT
ajlm-269	4	1	http://dx.doi.org/10.4102/ajlm.v5i1.269	http://dx.doi.org/10.4102/ajlm.v5i1.269	X
ajlm-269	4	2	original	original	ADJ
ajlm-269	4	3	research	research	NOUN
ajlm-269	4	4	real	real	ADJ
ajlm-269	4	5	-	-	PUNCT
ajlm-269	4	6	time	time	NOUN
ajlm-269	4	7	polymerase	polymerase	NOUN
ajlm-269	4	8	chain	chain	NOUN
ajlm-269	4	9	reaction	reaction	NOUN
ajlm-269	4	10	optimised	optimise	VERB
ajlm-269	4	11	for	for	ADP
ajlm-269	4	12	hepatitis	hepatitis	PROPN
ajlm-269	4	13	c	c	PROPN
ajlm-269	4	14	virus	virus	NOUN
ajlm-269	4	15	detection	detection	NOUN
ajlm-269	4	16	in	in	ADP
ajlm-269	4	17	dried	dry	VERB
ajlm-269	4	18	blood	blood	NOUN
ajlm-269	4	19	spots	spot	NOUN
ajlm-269	4	20	from	from	ADP
ajlm-269	4	21	hiv	hiv	NOUN
ajlm-269	4	22	-	-	PUNCT
ajlm-269	4	23	exposed	expose	VERB
ajlm-269	4	24	infants	infant	NOUN
ajlm-269	4	25	,	,	PUNCT
ajlm-269	4	26	kwazulu	kwazulu	NOUN
ajlm-269	4	27	-	-	PUNCT
ajlm-269	4	28	natal	natal	ADJ
ajlm-269	4	29	,	,	PUNCT
ajlm-269	4	30	south	south	PROPN
ajlm-269	4	31	africa	africa	PROPN
ajlm-269	4	32	anneta	anneta	PROPN
ajlm-269	4	33	naidoo	naidoo	PROPN
ajlm-269	4	34	,	,	PUNCT
ajlm-269	4	35	raveen	raveen	ADJ
ajlm-269	4	36	parboosing	parboose	VERB
ajlm-269	4	37	,	,	PUNCT
ajlm-269	4	38	pravi	pravi	PROPN
ajlm-269	4	39	moodley	moodley	PROPN
ajlm-269	4	40	received	receive	VERB
ajlm-269	4	41	:	:	PUNCT
ajlm-269	4	42	30	30	NUM
ajlm-269	4	43	sept	sept	PROPN
ajlm-269	4	44	.	.	PROPN
ajlm-269	4	45	2014	2014	NUM
ajlm-269	4	46	;	;	PUNCT
ajlm-269	4	47	accepted	accept	VERB
ajlm-269	4	48	:	:	PUNCT
ajlm-269	4	49	08	08	NUM
ajlm-269	4	50	jan	jan	PROPN
ajlm-269	4	51	.	.	PROPN
ajlm-269	4	52	2016	2016	NUM
ajlm-269	4	53	;	;	PUNCT
ajlm-269	4	54	published	publish	VERB
ajlm-269	4	55	:	:	PUNCT
ajlm-269	4	56	18	18	NUM
ajlm-269	4	57	mar	mar	PROPN
ajlm-269	4	58	.	.	PROPN
ajlm-269	5	1	2016	2016	NUM
ajlm-269	5	2	copyright	copyright	NOUN
ajlm-269	5	3	:	:	PUNCT
ajlm-269	5	4	©	©	PROPN
ajlm-269	5	5	2016	2016	NUM
ajlm-269	5	6	.	.	PUNCT
ajlm-269	6	1	the	the	DET
ajlm-269	6	2	author(s	author(s	PROPN
ajlm-269	6	3	)	)	PUNCT
ajlm-269	6	4	.	.	PUNCT
ajlm-269	7	1	licensee	licensee	NOUN
ajlm-269	7	2	:	:	PUNCT
ajlm-269	7	3	aosis	aosis	NOUN
ajlm-269	7	4	.	.	PUNCT
ajlm-269	8	1	this	this	PRON
ajlm-269	8	2	is	be	AUX
ajlm-269	8	3	an	an	DET
ajlm-269	8	4	open	open	ADJ
ajlm-269	8	5	access	access	NOUN
ajlm-269	8	6	article	article	NOUN
ajlm-269	8	7	distributed	distribute	VERB
ajlm-269	8	8	under	under	ADP
ajlm-269	8	9	the	the	DET
ajlm-269	8	10	terms	term	NOUN
ajlm-269	8	11	of	of	ADP
ajlm-269	8	12	the	the	DET
ajlm-269	8	13	creative	creative	ADJ
ajlm-269	8	14	commons	common	NOUN
ajlm-269	8	15	attribution	attribution	NOUN
ajlm-269	8	16	license	license	NOUN
ajlm-269	8	17	,	,	PUNCT
ajlm-269	8	18	which	which	PRON
ajlm-269	8	19	permits	permit	VERB
ajlm-269	8	20	unrestricted	unrestricted	ADJ
ajlm-269	8	21	use	use	NOUN
ajlm-269	8	22	,	,	PUNCT
ajlm-269	8	23	distribution	distribution	NOUN
ajlm-269	8	24	,	,	PUNCT
ajlm-269	8	25	and	and	CCONJ
ajlm-269	8	26	reproduction	reproduction	NOUN
ajlm-269	8	27	in	in	ADP
ajlm-269	8	28	any	any	DET
ajlm-269	8	29	medium	medium	NOUN
ajlm-269	8	30	,	,	PUNCT
ajlm-269	8	31	provided	provide	VERB
ajlm-269	8	32	the	the	DET
ajlm-269	8	33	original	original	ADJ
ajlm-269	8	34	work	work	NOUN
ajlm-269	8	35	is	be	AUX
ajlm-269	8	36	properly	properly	ADV
ajlm-269	8	37	cited	cite	VERB
ajlm-269	8	38	.	.	PUNCT
ajlm-269	9	1	abstract	abstract	ADJ
ajlm-269	9	2	background	background	NOUN
ajlm-269	9	3	:	:	PUNCT
ajlm-269	9	4	there	there	PRON
ajlm-269	9	5	is	be	VERB
ajlm-269	9	6	a	a	DET
ajlm-269	9	7	paucity	paucity	NOUN
ajlm-269	9	8	of	of	ADP
ajlm-269	9	9	data	datum	NOUN
ajlm-269	9	10	on	on	ADP
ajlm-269	9	11	the	the	DET
ajlm-269	9	12	prevalence	prevalence	NOUN
ajlm-269	9	13	of	of	ADP
ajlm-269	9	14	hepatitis	hepatitis	PROPN
ajlm-269	9	15	c	c	PROPN
ajlm-269	9	16	virus	virus	NOUN
ajlm-269	9	17	(	(	PUNCT
ajlm-269	9	18	hcv	hcv	PROPN
ajlm-269	9	19	)	)	PUNCT
ajlm-269	9	20	in	in	ADP
ajlm-269	9	21	children	child	NOUN
ajlm-269	9	22	,	,	PUNCT
ajlm-269	9	23	particularly	particularly	ADV
ajlm-269	9	24	in	in	ADP
ajlm-269	9	25	sub	sub	ADJ
ajlm-269	9	26	-	-	ADJ
ajlm-269	9	27	saharan	saharan	ADJ
ajlm-269	9	28	africa	africa	PROPN
ajlm-269	9	29	.	.	PUNCT
ajlm-269	10	1	a	a	DET
ajlm-269	10	2	major	major	ADJ
ajlm-269	10	3	obstacle	obstacle	NOUN
ajlm-269	10	4	in	in	ADP
ajlm-269	10	5	resource	resource	NOUN
ajlm-269	10	6	-	-	PUNCT
ajlm-269	10	7	limited	limit	VERB
ajlm-269	10	8	settings	setting	NOUN
ajlm-269	10	9	for	for	ADP
ajlm-269	10	10	polymerase	polymerase	NOUN
ajlm-269	10	11	chain	chain	NOUN
ajlm-269	10	12	reaction	reaction	NOUN
ajlm-269	10	13	(	(	PUNCT
ajlm-269	10	14	pcr	pcr	NOUN
ajlm-269	10	15	)	)	PUNCT
ajlm-269	10	16	testing	testing	NOUN
ajlm-269	10	17	is	be	AUX
ajlm-269	10	18	the	the	DET
ajlm-269	10	19	necessity	necessity	NOUN
ajlm-269	10	20	for	for	ADP
ajlm-269	10	21	specimen	speciman	NOUN
ajlm-269	10	22	transportation	transportation	NOUN
ajlm-269	10	23	and	and	CCONJ
ajlm-269	10	24	storage	storage	NOUN
ajlm-269	10	25	at	at	ADP
ajlm-269	10	26	low	low	ADJ
ajlm-269	10	27	temperatures	temperature	NOUN
ajlm-269	10	28	.	.	PUNCT
ajlm-269	11	1	there	there	PRON
ajlm-269	11	2	are	be	VERB
ajlm-269	11	3	numerous	numerous	ADJ
ajlm-269	11	4	recent	recent	ADJ
ajlm-269	11	5	studies	study	NOUN
ajlm-269	11	6	of	of	ADP
ajlm-269	11	7	using	use	VERB
ajlm-269	11	8	real	real	ADJ
ajlm-269	11	9	-	-	PUNCT
ajlm-269	11	10	time	time	NOUN
ajlm-269	11	11	hcv	hcv	X
ajlm-269	11	12	pcr	pcr	NOUN
ajlm-269	11	13	for	for	ADP
ajlm-269	11	14	diagnosis	diagnosis	NOUN
ajlm-269	11	15	and	and	CCONJ
ajlm-269	11	16	screening	screening	NOUN
ajlm-269	11	17	of	of	ADP
ajlm-269	11	18	plasma	plasma	NOUN
ajlm-269	11	19	and	and	CCONJ
ajlm-269	11	20	serum	serum	NOUN
ajlm-269	11	21	,	,	PUNCT
ajlm-269	11	22	but	but	CCONJ
ajlm-269	11	23	few	few	ADJ
ajlm-269	11	24	have	have	AUX
ajlm-269	11	25	looked	look	VERB
ajlm-269	11	26	at	at	ADP
ajlm-269	11	27	using	use	VERB
ajlm-269	11	28	dried	dry	VERB
ajlm-269	11	29	blood	blood	NOUN
ajlm-269	11	30	spot	spot	NOUN
ajlm-269	11	31	(	(	PUNCT
ajlm-269	11	32	dbs	dbs	PROPN
ajlm-269	11	33	)	)	PUNCT
ajlm-269	11	34	specimens	specimen	NOUN
ajlm-269	11	35	.	.	PUNCT
ajlm-269	12	1	objectives	objective	NOUN
ajlm-269	12	2	:	:	PUNCT
ajlm-269	12	3	the	the	DET
ajlm-269	12	4	aim	aim	NOUN
ajlm-269	12	5	of	of	ADP
ajlm-269	12	6	this	this	DET
ajlm-269	12	7	study	study	NOUN
ajlm-269	12	8	was	be	AUX
ajlm-269	12	9	to	to	PART
ajlm-269	12	10	optimise	optimise	VERB
ajlm-269	12	11	a	a	DET
ajlm-269	12	12	real	real	ADJ
ajlm-269	12	13	-	-	PUNCT
ajlm-269	12	14	time	time	NOUN
ajlm-269	12	15	hcv	hcv	X
ajlm-269	12	16	pcr	pcr	ADJ
ajlm-269	12	17	method	method	NOUN
ajlm-269	12	18	to	to	PART
ajlm-269	12	19	detect	detect	VERB
ajlm-269	12	20	hcv	hcv	PROPN
ajlm-269	12	21	rna	rna	PROPN
ajlm-269	12	22	from	from	ADP
ajlm-269	12	23	infant	infant	NOUN
ajlm-269	12	24	dbs	dbs	NOUN
ajlm-269	12	25	specimens	specimen	VERB
ajlm-269	12	26	for	for	ADP
ajlm-269	12	27	use	use	NOUN
ajlm-269	12	28	as	as	ADP
ajlm-269	12	29	a	a	DET
ajlm-269	12	30	tool	tool	NOUN
ajlm-269	12	31	for	for	ADP
ajlm-269	12	32	hcv	hcv	NOUN
ajlm-269	12	33	surveillance	surveillance	NOUN
ajlm-269	12	34	in	in	ADP
ajlm-269	12	35	kwazulu	kwazulu	NOUN
ajlm-269	12	36	-	-	PUNCT
ajlm-269	12	37	natal	natal	ADJ
ajlm-269	12	38	,	,	PUNCT
ajlm-269	12	39	south	south	PROPN
ajlm-269	12	40	africa	africa	PROPN
ajlm-269	12	41	.	.	PUNCT
ajlm-269	13	1	method	method	PROPN
ajlm-269	13	2	:	:	PUNCT
ajlm-269	13	3	the	the	DET
ajlm-269	13	4	lightcycler	lightcycler	ADJ
ajlm-269	13	5	®	®	NOUN
ajlm-269	13	6	2.0	2.0	NUM
ajlm-269	13	7	instrument	instrument	NOUN
ajlm-269	13	8	was	be	AUX
ajlm-269	13	9	used	use	VERB
ajlm-269	13	10	for	for	ADP
ajlm-269	13	11	the	the	DET
ajlm-269	13	12	hcv	hcv	PROPN
ajlm-269	13	13	pcr	pcr	NOUN
ajlm-269	13	14	using	use	VERB
ajlm-269	13	15	the	the	DET
ajlm-269	13	16	lightcycler	lightcycler	ADJ
ajlm-269	13	17	®	®	PROPN
ajlm-269	13	18	rna	rna	NOUN
ajlm-269	13	19	master	master	NOUN
ajlm-269	13	20	sybr	sybr	PROPN
ajlm-269	13	21	green	green	PROPN
ajlm-269	14	1	i	i	PROPN
ajlm-269	14	2	kit	kit	PROPN
ajlm-269	14	3	.	.	PUNCT
ajlm-269	15	1	template	template	NOUN
ajlm-269	15	2	volume	volume	NOUN
ajlm-269	15	3	,	,	PUNCT
ajlm-269	15	4	primer	primer	NOUN
ajlm-269	15	5	concentration	concentration	NOUN
ajlm-269	15	6	and	and	CCONJ
ajlm-269	15	7	primer	primer	NOUN
ajlm-269	15	8	annealing	anneal	VERB
ajlm-269	15	9	temperatures	temperature	NOUN
ajlm-269	15	10	were	be	AUX
ajlm-269	15	11	optimised	optimise	VERB
ajlm-269	15	12	and	and	CCONJ
ajlm-269	15	13	the	the	DET
ajlm-269	15	14	method	method	NOUN
ajlm-269	15	15	was	be	AUX
ajlm-269	15	16	used	use	VERB
ajlm-269	15	17	on	on	ADP
ajlm-269	15	18	179	179	NUM
ajlm-269	15	19	dbs	dbs	NOUN
ajlm-269	15	20	specimens	specimen	NOUN
ajlm-269	15	21	from	from	ADP
ajlm-269	15	22	hiv	hiv	PROPN
ajlm-269	15	23	-	-	PUNCT
ajlm-269	15	24	exposed	expose	VERB
ajlm-269	15	25	infants	infant	NOUN
ajlm-269	15	26	in	in	ADP
ajlm-269	15	27	kwazulu	kwazulu	NOUN
ajlm-269	15	28	-	-	PUNCT
ajlm-269	15	29	natal	natal	NOUN
ajlm-269	15	30	.	.	PUNCT
ajlm-269	16	1	results	result	NOUN
ajlm-269	16	2	:	:	PUNCT
ajlm-269	16	3	primer	primer	NOUN
ajlm-269	16	4	concentrations	concentration	NOUN
ajlm-269	16	5	adjusted	adjust	VERB
ajlm-269	16	6	to	to	ADP
ajlm-269	16	7	0.25	0.25	NUM
ajlm-269	16	8	µm	µm	NOUN
ajlm-269	16	9	and	and	CCONJ
ajlm-269	16	10	a	a	DET
ajlm-269	16	11	template	template	NOUN
ajlm-269	16	12	volume	volume	NOUN
ajlm-269	16	13	of	of	ADP
ajlm-269	16	14	10	10	NUM
ajlm-269	16	15	µl	µl	ADP
ajlm-269	16	16	improved	improve	VERB
ajlm-269	16	17	the	the	DET
ajlm-269	16	18	pcr	pcr	ADJ
ajlm-269	16	19	amplification	amplification	NOUN
ajlm-269	16	20	.	.	PUNCT
ajlm-269	17	1	primer	primer	NOUN
ajlm-269	17	2	annealing	anneal	VERB
ajlm-269	17	3	temperatures	temperature	NOUN
ajlm-269	17	4	lowered	lower	VERB
ajlm-269	17	5	from	from	ADP
ajlm-269	17	6	65	65	NUM
ajlm-269	17	7	°	°	NOUN
ajlm-269	17	8	c	c	NOUN
ajlm-269	17	9	to	to	ADP
ajlm-269	17	10	58	58	NUM
ajlm-269	17	11	°	°	NOUN
ajlm-269	17	12	c	c	NOUN
ajlm-269	17	13	resulted	result	VERB
ajlm-269	17	14	in	in	ADP
ajlm-269	17	15	higher	high	ADJ
ajlm-269	17	16	quantities	quantity	NOUN
ajlm-269	17	17	of	of	ADP
ajlm-269	17	18	amplified	amplify	VERB
ajlm-269	17	19	pcr	pcr	ADJ
ajlm-269	17	20	product	product	NOUN
ajlm-269	17	21	.	.	PUNCT
ajlm-269	18	1	the	the	DET
ajlm-269	18	2	limit	limit	NOUN
ajlm-269	18	3	of	of	ADP
ajlm-269	18	4	detection	detection	NOUN
ajlm-269	18	5	of	of	ADP
ajlm-269	18	6	the	the	DET
ajlm-269	18	7	optimised	optimise	VERB
ajlm-269	18	8	hcv	hcv	PROPN
ajlm-269	18	9	pcr	pcr	PROPN
ajlm-269	18	10	assay	assay	NOUN
ajlm-269	18	11	was	be	AUX
ajlm-269	18	12	between	between	ADP
ajlm-269	18	13	1200	1200	NUM
ajlm-269	18	14	iu	iu	ADP
ajlm-269	18	15	/	/	SYM
ajlm-269	18	16	ml	ml	NOUN
ajlm-269	18	17	and	and	CCONJ
ajlm-269	18	18	3580	3580	NUM
ajlm-269	18	19	iu	iu	NOUN
ajlm-269	18	20	/	/	SYM
ajlm-269	18	21	ml	ml	X
ajlm-269	18	22	of	of	ADP
ajlm-269	18	23	hcv	hcv	PROPN
ajlm-269	18	24	rna	rna	PROPN
ajlm-269	18	25	.	.	PUNCT
ajlm-269	19	1	hcv	hcv	PROPN
ajlm-269	19	2	was	be	AUX
ajlm-269	19	3	not	not	PART
ajlm-269	19	4	detected	detect	VERB
ajlm-269	19	5	in	in	ADP
ajlm-269	19	6	any	any	PRON
ajlm-269	19	7	of	of	ADP
ajlm-269	19	8	the	the	DET
ajlm-269	19	9	179	179	NUM
ajlm-269	19	10	dbs	dbs	PROPN
ajlm-269	19	11	specimens	specimen	NOUN
ajlm-269	19	12	.	.	PUNCT
ajlm-269	20	1	conclusion	conclusion	NOUN
ajlm-269	20	2	:	:	PUNCT
ajlm-269	20	3	the	the	DET
ajlm-269	20	4	optimised	optimise	VERB
ajlm-269	20	5	real	real	ADJ
ajlm-269	20	6	-	-	PUNCT
ajlm-269	20	7	time	time	NOUN
ajlm-269	20	8	hcv	hcv	X
ajlm-269	20	9	pcr	pcr	NOUN
ajlm-269	20	10	on	on	ADP
ajlm-269	20	11	infant	infant	NOUN
ajlm-269	20	12	dbs	dbs	PROPN
ajlm-269	20	13	specimens	specimen	NOUN
ajlm-269	20	14	performed	perform	VERB
ajlm-269	20	15	well	well	ADV
ajlm-269	20	16	,	,	PUNCT
ajlm-269	20	17	but	but	CCONJ
ajlm-269	20	18	hcv	hcv	PROPN
ajlm-269	20	19	was	be	AUX
ajlm-269	20	20	not	not	PART
ajlm-269	20	21	found	find	VERB
ajlm-269	20	22	in	in	ADP
ajlm-269	20	23	this	this	DET
ajlm-269	20	24	surveillance	surveillance	NOUN
ajlm-269	20	25	study	study	NOUN
ajlm-269	20	26	.	.	PUNCT
ajlm-269	21	1	hiv	hiv	PROPN
ajlm-269	21	2	infection	infection	NOUN
ajlm-269	21	3	may	may	AUX
ajlm-269	21	4	have	have	VERB
ajlm-269	21	5	little	little	ADJ
ajlm-269	21	6	impact	impact	NOUN
ajlm-269	21	7	on	on	ADP
ajlm-269	21	8	the	the	DET
ajlm-269	21	9	vertical	vertical	ADJ
ajlm-269	21	10	transmission	transmission	NOUN
ajlm-269	21	11	of	of	ADP
ajlm-269	21	12	hcv	hcv	NOUN
ajlm-269	21	13	in	in	ADP
ajlm-269	21	14	this	this	DET
ajlm-269	21	15	region	region	NOUN
ajlm-269	21	16	.	.	PUNCT
ajlm-269	22	1	introduction	introduction	NOUN
ajlm-269	22	2	hepatitis	hepatitis	PROPN
ajlm-269	22	3	c	c	PROPN
ajlm-269	22	4	virus	virus	PROPN
ajlm-269	22	5	(	(	PUNCT
ajlm-269	22	6	hcv	hcv	PROPN
ajlm-269	22	7	)	)	PUNCT
ajlm-269	22	8	infects	infect	VERB
ajlm-269	22	9	3	3	NUM
ajlm-269	22	10	%	%	NOUN
ajlm-269	22	11	of	of	ADP
ajlm-269	22	12	the	the	DET
ajlm-269	22	13	global	global	ADJ
ajlm-269	22	14	population,1	population,1	NOUN
ajlm-269	22	15	but	but	CCONJ
ajlm-269	22	16	there	there	PRON
ajlm-269	22	17	is	be	VERB
ajlm-269	22	18	a	a	DET
ajlm-269	22	19	paucity	paucity	NOUN
ajlm-269	22	20	of	of	ADP
ajlm-269	22	21	data	datum	NOUN
ajlm-269	22	22	on	on	ADP
ajlm-269	22	23	hcv	hcv	NOUN
ajlm-269	22	24	prevalence	prevalence	NOUN
ajlm-269	22	25	in	in	ADP
ajlm-269	22	26	children	child	NOUN
ajlm-269	22	27	,	,	PUNCT
ajlm-269	22	28	particularly	particularly	ADV
ajlm-269	22	29	children	child	NOUN
ajlm-269	22	30	in	in	ADP
ajlm-269	22	31	sub	sub	ADJ
ajlm-269	22	32	-	-	ADJ
ajlm-269	22	33	saharan	saharan	ADJ
ajlm-269	22	34	africa.2	africa.2	PROPN
ajlm-269	22	35	hcv	hcv	PROPN
ajlm-269	22	36	is	be	AUX
ajlm-269	22	37	usually	usually	ADV
ajlm-269	22	38	transmitted	transmit	VERB
ajlm-269	22	39	to	to	ADP
ajlm-269	22	40	infants	infant	NOUN
ajlm-269	22	41	vertically	vertically	ADV
ajlm-269	22	42	,	,	PUNCT
ajlm-269	22	43	with	with	ADP
ajlm-269	22	44	a	a	DET
ajlm-269	22	45	rate	rate	NOUN
ajlm-269	22	46	that	that	PRON
ajlm-269	22	47	varies	vary	VERB
ajlm-269	22	48	from	from	ADP
ajlm-269	22	49	3	3	NUM
ajlm-269	22	50	%	%	NOUN
ajlm-269	22	51	to	to	PART
ajlm-269	22	52	7	7	NUM
ajlm-269	22	53	%	%	NOUN
ajlm-269	22	54	,	,	PUNCT
ajlm-269	22	55	although	although	SCONJ
ajlm-269	22	56	it	it	PRON
ajlm-269	22	57	may	may	AUX
ajlm-269	22	58	increase	increase	VERB
ajlm-269	22	59	twoto	twoto	PRON
ajlm-269	22	60	five	five	NUM
ajlm-269	22	61	-	-	NOUN
ajlm-269	22	62	fold	fold	ADJ
ajlm-269	22	63	with	with	ADP
ajlm-269	22	64	hiv	hiv	PROPN
ajlm-269	22	65	co	co	PROPN
ajlm-269	22	66	-	-	PROPN
ajlm-269	22	67	infection.3	infection.3	PROPN
ajlm-269	22	68	the	the	DET
ajlm-269	22	69	prevalence	prevalence	NOUN
ajlm-269	22	70	of	of	ADP
ajlm-269	22	71	hcv	hcv	NOUN
ajlm-269	22	72	is	be	AUX
ajlm-269	22	73	higher	high	ADJ
ajlm-269	22	74	amongst	amongst	ADP
ajlm-269	22	75	hiv	hiv	PROPN
ajlm-269	22	76	-	-	PUNCT
ajlm-269	22	77	positive	positive	ADJ
ajlm-269	22	78	patients	patient	NOUN
ajlm-269	22	79	because	because	SCONJ
ajlm-269	22	80	of	of	ADP
ajlm-269	22	81	shared	share	VERB
ajlm-269	22	82	routes	route	NOUN
ajlm-269	22	83	of	of	ADP
ajlm-269	22	84	transmission	transmission	NOUN
ajlm-269	22	85	and	and	CCONJ
ajlm-269	22	86	common	common	ADJ
ajlm-269	22	87	risk	risk	NOUN
ajlm-269	22	88	factors.4	factors.4	PROPN
ajlm-269	22	89	the	the	DET
ajlm-269	22	90	estimated	estimate	VERB
ajlm-269	22	91	prevalence	prevalence	NOUN
ajlm-269	22	92	of	of	ADP
ajlm-269	22	93	hcv	hcv	NOUN
ajlm-269	22	94	in	in	ADP
ajlm-269	22	95	sub	sub	ADJ
ajlm-269	22	96	-	-	ADJ
ajlm-269	22	97	saharan	saharan	ADJ
ajlm-269	22	98	africa	africa	PROPN
ajlm-269	22	99	was	be	AUX
ajlm-269	22	100	3	3	NUM
ajlm-269	22	101	%	%	NOUN
ajlm-269	22	102	amongst	amongst	ADP
ajlm-269	22	103	hiv	hiv	PROPN
ajlm-269	22	104	-	-	PUNCT
ajlm-269	22	105	negative	negative	ADJ
ajlm-269	22	106	individuals	individual	NOUN
ajlm-269	22	107	in	in	ADP
ajlm-269	22	108	2002	2002	NUM
ajlm-269	22	109	,	,	PUNCT
ajlm-269	22	110	but	but	CCONJ
ajlm-269	22	111	prevalence	prevalence	NOUN
ajlm-269	22	112	increased	increase	VERB
ajlm-269	22	113	to	to	ADP
ajlm-269	22	114	7	7	NUM
ajlm-269	22	115	%	%	NOUN
ajlm-269	22	116	amongst	amongst	ADP
ajlm-269	22	117	individuals	individual	NOUN
ajlm-269	22	118	with	with	ADP
ajlm-269	22	119	hiv	hiv	PROPN
ajlm-269	22	120	co	co	NOUN
ajlm-269	22	121	-	-	NOUN
ajlm-269	22	122	infection	infection	NOUN
ajlm-269	22	123	by	by	ADP
ajlm-269	22	124	2014.5	2014.5	NUM
ajlm-269	22	125	the	the	DET
ajlm-269	22	126	prevalence	prevalence	NOUN
ajlm-269	22	127	of	of	ADP
ajlm-269	22	128	hcv	hcv	PROPN
ajlm-269	22	129	/	/	SYM
ajlm-269	22	130	hiv	hiv	PROPN
ajlm-269	22	131	co	co	NOUN
ajlm-269	22	132	-	-	NOUN
ajlm-269	22	133	infection	infection	NOUN
ajlm-269	22	134	in	in	ADP
ajlm-269	22	135	south	south	PROPN
ajlm-269	22	136	africa	africa	PROPN
ajlm-269	22	137	was	be	AUX
ajlm-269	22	138	0.1	0.1	NUM
ajlm-269	22	139	%	%	NOUN
ajlm-269	22	140	in	in	ADP
ajlm-269	22	141	20026	20026	NUM
ajlm-269	22	142	compared	compare	VERB
ajlm-269	22	143	to	to	ADP
ajlm-269	22	144	3.5	3.5	NUM
ajlm-269	22	145	%	%	NOUN
ajlm-269	22	146	amongst	amongst	ADP
ajlm-269	22	147	individuals	individual	NOUN
ajlm-269	22	148	infected	infect	VERB
ajlm-269	22	149	only	only	ADV
ajlm-269	22	150	with	with	ADP
ajlm-269	22	151	hcv.7	hcv.7	PROPN
ajlm-269	22	152	there	there	PRON
ajlm-269	22	153	are	be	VERB
ajlm-269	22	154	reasons	reason	NOUN
ajlm-269	22	155	to	to	PART
ajlm-269	22	156	suspect	suspect	VERB
ajlm-269	22	157	that	that	SCONJ
ajlm-269	22	158	the	the	DET
ajlm-269	22	159	prevalence	prevalence	NOUN
ajlm-269	22	160	of	of	ADP
ajlm-269	22	161	hcv	hcv	NOUN
ajlm-269	22	162	may	may	AUX
ajlm-269	22	163	be	be	AUX
ajlm-269	22	164	high	high	ADJ
ajlm-269	22	165	in	in	ADP
ajlm-269	22	166	kwazulu	kwazulu	NOUN
ajlm-269	22	167	-	-	PUNCT
ajlm-269	22	168	natal	natal	ADJ
ajlm-269	22	169	,	,	PUNCT
ajlm-269	22	170	south	south	PROPN
ajlm-269	22	171	africa	africa	PROPN
ajlm-269	22	172	.	.	PUNCT
ajlm-269	23	1	firstly	firstly	ADV
ajlm-269	23	2	,	,	PUNCT
ajlm-269	23	3	kwazulu	kwazulu	PROPN
ajlm-269	23	4	-	-	PUNCT
ajlm-269	23	5	natal	natal	NOUN
ajlm-269	23	6	has	have	VERB
ajlm-269	23	7	a	a	DET
ajlm-269	23	8	high	high	ADJ
ajlm-269	23	9	antenatal	antenatal	NOUN
ajlm-269	23	10	hiv	hiv	PROPN
ajlm-269	23	11	prevalence	prevalence	NOUN
ajlm-269	23	12	of	of	ADP
ajlm-269	23	13	40.1%.8	40.1%.8	PROPN
ajlm-269	23	14	secondly	secondly	ADV
ajlm-269	23	15	,	,	PUNCT
ajlm-269	23	16	kwazulu	kwazulu	PROPN
ajlm-269	23	17	-	-	PUNCT
ajlm-269	23	18	natal	natal	NOUN
ajlm-269	23	19	has	have	VERB
ajlm-269	23	20	a	a	DET
ajlm-269	23	21	high	high	ADJ
ajlm-269	23	22	overall	overall	ADJ
ajlm-269	23	23	adult	adult	NOUN
ajlm-269	23	24	hcv	hcv	NOUN
ajlm-269	23	25	prevalence	prevalence	NOUN
ajlm-269	23	26	of	of	ADP
ajlm-269	23	27	6.4	6.4	NUM
ajlm-269	23	28	%	%	NOUN
ajlm-269	23	29	and	and	CCONJ
ajlm-269	23	30	a	a	DET
ajlm-269	23	31	much	much	ADV
ajlm-269	23	32	higher	high	ADJ
ajlm-269	23	33	prevalence	prevalence	NOUN
ajlm-269	23	34	of	of	ADP
ajlm-269	23	35	hcv	hcv	PROPN
ajlm-269	23	36	/	/	SYM
ajlm-269	23	37	hiv	hiv	PROPN
ajlm-269	23	38	co	co	NOUN
ajlm-269	23	39	-	-	NOUN
ajlm-269	23	40	infection	infection	NOUN
ajlm-269	23	41	(	(	PUNCT
ajlm-269	23	42	13.4%),9	13.4%),9	NUM
ajlm-269	23	43	which	which	PRON
ajlm-269	23	44	may	may	AUX
ajlm-269	23	45	imply	imply	VERB
ajlm-269	23	46	a	a	DET
ajlm-269	23	47	high	high	ADJ
ajlm-269	23	48	prevalence	prevalence	NOUN
ajlm-269	23	49	of	of	ADP
ajlm-269	23	50	hcv	hcv	PROPN
ajlm-269	23	51	amongst	amongst	ADP
ajlm-269	23	52	pregnant	pregnant	ADJ
ajlm-269	23	53	woman	woman	NOUN
ajlm-269	23	54	.	.	PUNCT
ajlm-269	24	1	the	the	DET
ajlm-269	24	2	world	world	PROPN
ajlm-269	24	3	health	health	PROPN
ajlm-269	24	4	organization	organization	NOUN
ajlm-269	24	5	suggests	suggest	VERB
ajlm-269	24	6	that	that	SCONJ
ajlm-269	24	7	screening	screen	VERB
ajlm-269	24	8	for	for	ADP
ajlm-269	24	9	hcv	hcv	PROPN
ajlm-269	24	10	in	in	ADP
ajlm-269	24	11	regions	region	NOUN
ajlm-269	24	12	with	with	ADP
ajlm-269	24	13	a	a	DET
ajlm-269	24	14	high	high	ADJ
ajlm-269	24	15	prevalence	prevalence	NOUN
ajlm-269	24	16	of	of	ADP
ajlm-269	24	17	hiv	hiv	PROPN
ajlm-269	24	18	should	should	AUX
ajlm-269	24	19	be	be	AUX
ajlm-269	24	20	conducted	conduct	VERB
ajlm-269	24	21	,	,	PUNCT
ajlm-269	24	22	but	but	CCONJ
ajlm-269	24	23	depends	depend	VERB
ajlm-269	24	24	on	on	ADP
ajlm-269	24	25	hcv	hcv	NOUN
ajlm-269	24	26	prevalence	prevalence	NOUN
ajlm-269	24	27	and	and	CCONJ
ajlm-269	24	28	resources.10	resources.10	NOUN
ajlm-269	24	29	in	in	ADP
ajlm-269	24	30	a	a	DET
ajlm-269	24	31	region	region	NOUN
ajlm-269	24	32	with	with	ADP
ajlm-269	24	33	a	a	DET
ajlm-269	24	34	high	high	ADJ
ajlm-269	24	35	prevalence	prevalence	NOUN
ajlm-269	24	36	of	of	ADP
ajlm-269	24	37	both	both	DET
ajlm-269	24	38	hiv	hiv	PROPN
ajlm-269	24	39	and	and	CCONJ
ajlm-269	24	40	hcv	hcv	PROPN
ajlm-269	24	41	,	,	PUNCT
ajlm-269	24	42	especially	especially	ADV
ajlm-269	24	43	amongst	amongst	ADP
ajlm-269	24	44	pregnant	pregnant	ADJ
ajlm-269	24	45	women,9	women,9	NOUN
ajlm-269	24	46	it	it	PRON
ajlm-269	24	47	is	be	AUX
ajlm-269	24	48	important	important	ADJ
ajlm-269	24	49	to	to	PART
ajlm-269	24	50	determine	determine	VERB
ajlm-269	24	51	the	the	DET
ajlm-269	24	52	prevalence	prevalence	NOUN
ajlm-269	24	53	of	of	ADP
ajlm-269	24	54	hcv	hcv	NOUN
ajlm-269	24	55	amongst	amongst	ADP
ajlm-269	24	56	infants	infant	NOUN
ajlm-269	24	57	.	.	PUNCT
ajlm-269	25	1	although	although	SCONJ
ajlm-269	25	2	most	most	ADJ
ajlm-269	25	3	children	child	NOUN
ajlm-269	25	4	with	with	ADP
ajlm-269	25	5	chronic	chronic	ADJ
ajlm-269	25	6	hcv	hcv	NOUN
ajlm-269	25	7	infection	infection	NOUN
ajlm-269	25	8	remain	remain	VERB
ajlm-269	25	9	asymptomatic	asymptomatic	ADJ
ajlm-269	25	10	,	,	PUNCT
ajlm-269	25	11	some	some	PRON
ajlm-269	25	12	may	may	AUX
ajlm-269	25	13	have	have	VERB
ajlm-269	25	14	mild	mild	ADJ
ajlm-269	25	15	abnormalities	abnormality	NOUN
ajlm-269	25	16	in	in	ADP
ajlm-269	25	17	liver	liver	NOUN
ajlm-269	25	18	function	function	NOUN
ajlm-269	25	19	and	and	CCONJ
ajlm-269	25	20	chronic	chronic	ADJ
ajlm-269	25	21	hcv	hcv	NOUN
ajlm-269	25	22	infection	infection	NOUN
ajlm-269	25	23	may	may	AUX
ajlm-269	25	24	progress	progress	VERB
ajlm-269	25	25	to	to	ADP
ajlm-269	25	26	severe	severe	ADJ
ajlm-269	25	27	liver	liver	NOUN
ajlm-269	25	28	disease	disease	NOUN
ajlm-269	25	29	,	,	PUNCT
ajlm-269	25	30	end	end	NOUN
ajlm-269	25	31	-	-	PUNCT
ajlm-269	25	32	stage	stage	NOUN
ajlm-269	25	33	liver	liver	NOUN
ajlm-269	25	34	disease	disease	NOUN
ajlm-269	25	35	and	and	CCONJ
ajlm-269	25	36	death.2	death.2	PROPN
ajlm-269	25	37	liver	liver	NOUN
ajlm-269	25	38	disease	disease	NOUN
ajlm-269	25	39	resulting	result	VERB
ajlm-269	25	40	from	from	ADP
ajlm-269	25	41	hcv	hcv	ADJ
ajlm-269	25	42	infection	infection	NOUN
ajlm-269	25	43	is	be	AUX
ajlm-269	25	44	a	a	DET
ajlm-269	25	45	major	major	ADJ
ajlm-269	25	46	cause	cause	NOUN
ajlm-269	25	47	of	of	ADP
ajlm-269	25	48	mortality	mortality	NOUN
ajlm-269	25	49	and	and	CCONJ
ajlm-269	25	50	morbidity	morbidity	NOUN
ajlm-269	25	51	amongst	amongst	ADP
ajlm-269	25	52	adults	adult	NOUN
ajlm-269	25	53	co	co	VERB
ajlm-269	25	54	-	-	VERB
ajlm-269	25	55	infected	infected	ADJ
ajlm-269	25	56	with	with	ADP
ajlm-269	25	57	hiv	hiv	PROPN
ajlm-269	25	58	and	and	CCONJ
ajlm-269	25	59	hcv	hcv	PROPN
ajlm-269	25	60	,	,	PUNCT
ajlm-269	25	61	which	which	PRON
ajlm-269	25	62	further	far	ADV
ajlm-269	25	63	compounds	compound	VERB
ajlm-269	25	64	the	the	DET
ajlm-269	25	65	hepatotoxic	hepatotoxic	ADJ
ajlm-269	25	66	effects	effect	NOUN
ajlm-269	25	67	of	of	ADP
ajlm-269	25	68	antiretroviral	antiretroviral	ADJ
ajlm-269	25	69	therapy.4	therapy.4	NOUN
ajlm-269	25	70	infant	infant	NOUN
ajlm-269	25	71	diagnosis	diagnosis	NOUN
ajlm-269	25	72	of	of	ADP
ajlm-269	25	73	hcv	hcv	NOUN
ajlm-269	25	74	by	by	ADP
ajlm-269	25	75	molecular	molecular	NOUN
ajlm-269	25	76	-	-	PUNCT
ajlm-269	25	77	based	base	VERB
ajlm-269	25	78	technologies	technology	NOUN
ajlm-269	25	79	is	be	AUX
ajlm-269	25	80	superior	superior	ADJ
ajlm-269	25	81	to	to	ADP
ajlm-269	25	82	serological	serological	ADJ
ajlm-269	25	83	techniques	technique	NOUN
ajlm-269	25	84	for	for	ADP
ajlm-269	25	85	detecting	detect	VERB
ajlm-269	25	86	hcv	hcv	NOUN
ajlm-269	25	87	infection	infection	NOUN
ajlm-269	25	88	by	by	ADP
ajlm-269	25	89	means	mean	NOUN
ajlm-269	25	90	of	of	ADP
ajlm-269	25	91	maternal	maternal	ADJ
ajlm-269	25	92	antibodies	antibody	NOUN
ajlm-269	25	93	circulating	circulate	VERB
ajlm-269	25	94	in	in	ADP
ajlm-269	25	95	infants.2	infants.2	ADP
ajlm-269	25	96	a	a	DET
ajlm-269	25	97	major	major	ADJ
ajlm-269	25	98	obstacle	obstacle	NOUN
ajlm-269	25	99	in	in	ADP
ajlm-269	25	100	resource	resource	NOUN
ajlm-269	25	101	-	-	PUNCT
ajlm-269	25	102	limited	limit	VERB
ajlm-269	25	103	settings	setting	NOUN
ajlm-269	25	104	for	for	ADP
ajlm-269	25	105	polymerase	polymerase	NOUN
ajlm-269	25	106	chain	chain	NOUN
ajlm-269	25	107	reaction	reaction	NOUN
ajlm-269	25	108	(	(	PUNCT
ajlm-269	25	109	pcr	pcr	NOUN
ajlm-269	25	110	)	)	PUNCT
ajlm-269	25	111	testing	testing	NOUN
ajlm-269	25	112	is	be	AUX
ajlm-269	25	113	the	the	DET
ajlm-269	25	114	necessity	necessity	NOUN
ajlm-269	25	115	of	of	ADP
ajlm-269	25	116	transporting	transport	VERB
ajlm-269	25	117	and	and	CCONJ
ajlm-269	25	118	storing	store	VERB
ajlm-269	25	119	specimens	specimen	NOUN
ajlm-269	25	120	at	at	ADP
ajlm-269	25	121	low	low	ADJ
ajlm-269	25	122	temperatures	temperature	NOUN
ajlm-269	25	123	.	.	PUNCT
ajlm-269	26	1	however	however	ADV
ajlm-269	26	2	,	,	PUNCT
ajlm-269	26	3	using	use	VERB
ajlm-269	26	4	dried	dry	VERB
ajlm-269	26	5	blood	blood	NOUN
ajlm-269	26	6	spot	spot	NOUN
ajlm-269	26	7	(	(	PUNCT
ajlm-269	26	8	dbs	dbs	PROPN
ajlm-269	26	9	)	)	PUNCT
ajlm-269	26	10	specimens	specimen	NOUN
ajlm-269	26	11	,	,	PUNCT
ajlm-269	26	12	which	which	PRON
ajlm-269	26	13	do	do	AUX
ajlm-269	26	14	not	not	PART
ajlm-269	26	15	require	require	VERB
ajlm-269	26	16	refrigeration	refrigeration	NOUN
ajlm-269	26	17	,	,	PUNCT
ajlm-269	26	18	significantly	significantly	ADV
ajlm-269	26	19	reduces	reduce	VERB
ajlm-269	26	20	pre	pre	ADJ
ajlm-269	26	21	-	-	ADJ
ajlm-269	26	22	analytical	analytical	ADJ
ajlm-269	26	23	problems	problem	NOUN
ajlm-269	26	24	and	and	CCONJ
ajlm-269	26	25	is	be	AUX
ajlm-269	26	26	widely	widely	ADV
ajlm-269	26	27	used	use	VERB
ajlm-269	26	28	in	in	ADP
ajlm-269	26	29	molecular	molecular	ADJ
ajlm-269	26	30	virology	virology	NOUN
ajlm-269	26	31	for	for	ADP
ajlm-269	26	32	the	the	DET
ajlm-269	26	33	detection	detection	NOUN
ajlm-269	26	34	of	of	ADP
ajlm-269	26	35	viral	viral	ADJ
ajlm-269	26	36	nucleic	nucleic	ADJ
ajlm-269	26	37	acids.11	acids.11	ADJ
ajlm-269	26	38	numerous	numerous	ADJ
ajlm-269	26	39	studies	study	NOUN
ajlm-269	26	40	have	have	AUX
ajlm-269	26	41	reported	report	VERB
ajlm-269	26	42	on	on	ADP
ajlm-269	26	43	the	the	DET
ajlm-269	26	44	use	use	NOUN
ajlm-269	26	45	of	of	ADP
ajlm-269	26	46	real	real	ADJ
ajlm-269	26	47	-	-	PUNCT
ajlm-269	26	48	time	time	NOUN
ajlm-269	26	49	pcr	pcr	NOUN
ajlm-269	26	50	for	for	ADP
ajlm-269	26	51	hcv	hcv	NOUN
ajlm-269	26	52	diagnosis	diagnosis	NOUN
ajlm-269	26	53	using	use	VERB
ajlm-269	26	54	plasma	plasma	NOUN
ajlm-269	26	55	and	and	CCONJ
ajlm-269	26	56	serum,12,13,14,15,16,17,18,19,20	serum,12,13,14,15,16,17,18,19,20	PRON
ajlm-269	26	57	although	although	SCONJ
ajlm-269	26	58	few	few	ADJ
ajlm-269	26	59	have	have	AUX
ajlm-269	26	60	looked	look	VERB
ajlm-269	26	61	at	at	ADP
ajlm-269	26	62	dbs	dbs	PROPN
ajlm-269	26	63	specimens.21,22,23,24,25	specimens.21,22,23,24,25	PROPN
ajlm-269	26	64	however	however	ADV
ajlm-269	26	65	,	,	PUNCT
ajlm-269	26	66	a	a	DET
ajlm-269	26	67	few	few	ADJ
ajlm-269	26	68	recent	recent	ADJ
ajlm-269	26	69	studies	study	NOUN
ajlm-269	26	70	have	have	AUX
ajlm-269	26	71	found	find	VERB
ajlm-269	26	72	good	good	ADJ
ajlm-269	26	73	correlations	correlation	NOUN
ajlm-269	26	74	for	for	ADP
ajlm-269	26	75	adult	adult	NOUN
ajlm-269	26	76	serum	serum	NOUN
ajlm-269	26	77	and	and	CCONJ
ajlm-269	26	78	plasma	plasma	NOUN
ajlm-269	26	79	compared	compare	VERB
ajlm-269	26	80	with	with	ADP
ajlm-269	26	81	dbs	dbs	PROPN
ajlm-269	26	82	specimens	specimen	NOUN
ajlm-269	26	83	analysed	analyse	VERB
ajlm-269	26	84	using	use	VERB
ajlm-269	26	85	commercial	commercial	ADJ
ajlm-269	26	86	hcv	hcv	NOUN
ajlm-269	26	87	pcr	pcr	NOUN
ajlm-269	26	88	assays.26,27,28,29	assays.26,27,28,29	PROPN
ajlm-269	26	89	the	the	DET
ajlm-269	26	90	prevalence	prevalence	NOUN
ajlm-269	26	91	of	of	ADP
ajlm-269	26	92	hcv	hcv	PROPN
ajlm-269	26	93	amongst	amongst	ADP
ajlm-269	26	94	hiv	hiv	PROPN
ajlm-269	26	95	-	-	PUNCT
ajlm-269	26	96	exposed	expose	VERB
ajlm-269	26	97	infants	infant	NOUN
ajlm-269	26	98	in	in	ADP
ajlm-269	26	99	kwazulu	kwazulu	NOUN
ajlm-269	26	100	-	-	PUNCT
ajlm-269	26	101	natal	natal	ADJ
ajlm-269	26	102	,	,	PUNCT
ajlm-269	26	103	south	south	PROPN
ajlm-269	26	104	africa	africa	PROPN
ajlm-269	26	105	is	be	AUX
ajlm-269	26	106	unknown	unknown	ADJ
ajlm-269	26	107	.	.	PUNCT
ajlm-269	27	1	therefore	therefore	ADV
ajlm-269	27	2	,	,	PUNCT
ajlm-269	27	3	the	the	DET
ajlm-269	27	4	aim	aim	NOUN
ajlm-269	27	5	of	of	ADP
ajlm-269	27	6	this	this	DET
ajlm-269	27	7	study	study	NOUN
ajlm-269	27	8	was	be	AUX
ajlm-269	27	9	to	to	PART
ajlm-269	27	10	optimise	optimise	VERB
ajlm-269	27	11	a	a	DET
ajlm-269	27	12	real	real	ADJ
ajlm-269	27	13	-	-	PUNCT
ajlm-269	27	14	time	time	NOUN
ajlm-269	27	15	pcr	pcr	PROPN
ajlm-269	27	16	assay	assay	NOUN
ajlm-269	27	17	to	to	PART
ajlm-269	27	18	detect	detect	VERB
ajlm-269	27	19	hcv	hcv	PROPN
ajlm-269	27	20	rna	rna	PROPN
ajlm-269	27	21	from	from	ADP
ajlm-269	27	22	hiv	hiv	PROPN
ajlm-269	27	23	-	-	PUNCT
ajlm-269	27	24	exposed	expose	VERB
ajlm-269	27	25	infant	infant	NOUN
ajlm-269	27	26	dbs	dbs	NOUN
ajlm-269	27	27	specimens	specimen	NOUN
ajlm-269	27	28	for	for	ADP
ajlm-269	27	29	use	use	NOUN
ajlm-269	27	30	as	as	ADP
ajlm-269	27	31	a	a	DET
ajlm-269	27	32	tool	tool	NOUN
ajlm-269	27	33	for	for	ADP
ajlm-269	27	34	hcv	hcv	NOUN
ajlm-269	27	35	surveillance	surveillance	NOUN
ajlm-269	27	36	in	in	ADP
ajlm-269	27	37	kwazulu	kwazulu	NOUN
ajlm-269	27	38	-	-	PUNCT
ajlm-269	27	39	natal	natal	ADJ
ajlm-269	27	40	,	,	PUNCT
ajlm-269	27	41	south	south	PROPN
ajlm-269	27	42	africa	africa	PROPN
ajlm-269	27	43	.	.	PUNCT
ajlm-269	28	1	research	research	NOUN
ajlm-269	28	2	method	method	NOUN
ajlm-269	28	3	and	and	CCONJ
ajlm-269	28	4	design	design	VERB
ajlm-269	28	5	ethical	ethical	ADJ
ajlm-269	28	6	considerations	consideration	NOUN
ajlm-269	28	7	ethical	ethical	ADJ
ajlm-269	28	8	clearance	clearance	NOUN
ajlm-269	28	9	was	be	AUX
ajlm-269	28	10	obtained	obtain	VERB
ajlm-269	28	11	from	from	ADP
ajlm-269	28	12	the	the	DET
ajlm-269	28	13	university	university	NOUN
ajlm-269	28	14	of	of	ADP
ajlm-269	28	15	kwazulu	kwazulu	PROPN
ajlm-269	28	16	-	-	PUNCT
ajlm-269	28	17	natal	natal	ADJ
ajlm-269	28	18	biomedical	biomedical	ADJ
ajlm-269	28	19	research	research	NOUN
ajlm-269	28	20	ethics	ethic	NOUN
ajlm-269	28	21	committee	committee	PROPN
ajlm-269	28	22	(	(	PUNCT
ajlm-269	28	23	be273/090	be273/090	PROPN
ajlm-269	28	24	)	)	PUNCT
ajlm-269	28	25	.	.	PUNCT
ajlm-269	29	1	informed	informed	ADJ
ajlm-269	29	2	consent	consent	NOUN
ajlm-269	29	3	was	be	AUX
ajlm-269	29	4	not	not	PART
ajlm-269	29	5	required	require	VERB
ajlm-269	29	6	since	since	SCONJ
ajlm-269	29	7	this	this	PRON
ajlm-269	29	8	was	be	AUX
ajlm-269	29	9	a	a	DET
ajlm-269	29	10	retrospective	retrospective	ADJ
ajlm-269	29	11	study	study	NOUN
ajlm-269	29	12	in	in	ADP
ajlm-269	29	13	which	which	PRON
ajlm-269	29	14	we	we	PRON
ajlm-269	29	15	used	use	VERB
ajlm-269	29	16	discarded	discard	VERB
ajlm-269	29	17	specimens	specimen	NOUN
ajlm-269	29	18	from	from	ADP
ajlm-269	29	19	routine	routine	ADJ
ajlm-269	29	20	testing	testing	NOUN
ajlm-269	29	21	.	.	PUNCT
ajlm-269	30	1	all	all	DET
ajlm-269	30	2	data	datum	NOUN
ajlm-269	30	3	were	be	AUX
ajlm-269	30	4	recorded	record	VERB
ajlm-269	30	5	and	and	CCONJ
ajlm-269	30	6	then	then	ADV
ajlm-269	30	7	anonymised	anonymise	VERB
ajlm-269	30	8	for	for	ADP
ajlm-269	30	9	the	the	DET
ajlm-269	30	10	purposes	purpose	NOUN
ajlm-269	30	11	of	of	ADP
ajlm-269	30	12	the	the	DET
ajlm-269	30	13	study	study	NOUN
ajlm-269	30	14	.	.	PUNCT
ajlm-269	31	1	specifically	specifically	ADV
ajlm-269	31	2	,	,	PUNCT
ajlm-269	31	3	hiv	hiv	PROPN
ajlm-269	31	4	pcr	pcr	PROPN
ajlm-269	31	5	results	result	NOUN
ajlm-269	31	6	,	,	PUNCT
ajlm-269	31	7	hcv	hcv	VERB
ajlm-269	31	8	pcr	pcr	ADJ
ajlm-269	31	9	results	result	NOUN
ajlm-269	31	10	,	,	PUNCT
ajlm-269	31	11	age	age	NOUN
ajlm-269	31	12	and	and	CCONJ
ajlm-269	31	13	clinical	clinical	ADJ
ajlm-269	31	14	diagnosis	diagnosis	NOUN
ajlm-269	31	15	were	be	AUX
ajlm-269	31	16	recorded	record	VERB
ajlm-269	31	17	anonymously	anonymously	ADV
ajlm-269	31	18	in	in	ADP
ajlm-269	31	19	a	a	DET
ajlm-269	31	20	spreadsheet	spreadsheet	NOUN
ajlm-269	31	21	.	.	PUNCT
ajlm-269	32	1	no	no	DET
ajlm-269	32	2	patient	patient	ADJ
ajlm-269	32	3	identifiers	identifier	NOUN
ajlm-269	32	4	were	be	AUX
ajlm-269	32	5	recorded	record	VERB
ajlm-269	32	6	.	.	PUNCT
ajlm-269	33	1	study	study	NOUN
ajlm-269	33	2	design	design	NOUN
ajlm-269	33	3	this	this	DET
ajlm-269	33	4	study	study	NOUN
ajlm-269	33	5	was	be	AUX
ajlm-269	33	6	conducted	conduct	VERB
ajlm-269	33	7	retrospectively	retrospectively	ADV
ajlm-269	33	8	in	in	ADP
ajlm-269	33	9	the	the	DET
ajlm-269	33	10	laboratory	laboratory	NOUN
ajlm-269	33	11	at	at	ADP
ajlm-269	33	12	the	the	DET
ajlm-269	33	13	department	department	NOUN
ajlm-269	33	14	of	of	ADP
ajlm-269	33	15	virology	virology	NOUN
ajlm-269	33	16	,	,	PUNCT
ajlm-269	33	17	national	national	PROPN
ajlm-269	33	18	health	health	PROPN
ajlm-269	33	19	laboratory	laboratory	PROPN
ajlm-269	33	20	service	service	PROPN
ajlm-269	33	21	/	/	SYM
ajlm-269	33	22	university	university	PROPN
ajlm-269	33	23	of	of	ADP
ajlm-269	33	24	kwazulu	kwazulu	PROPN
ajlm-269	33	25	-	-	PUNCT
ajlm-269	33	26	natal	natal	NOUN
ajlm-269	33	27	,	,	PUNCT
ajlm-269	33	28	at	at	ADP
ajlm-269	33	29	the	the	DET
ajlm-269	33	30	inkosi	inkosi	PROPN
ajlm-269	33	31	albert	albert	PROPN
ajlm-269	33	32	luthuli	luthuli	PROPN
ajlm-269	33	33	central	central	PROPN
ajlm-269	33	34	hospital	hospital	NOUN
ajlm-269	33	35	in	in	ADP
ajlm-269	33	36	durban	durban	PROPN
ajlm-269	33	37	,	,	PUNCT
ajlm-269	33	38	south	south	PROPN
ajlm-269	33	39	africa	africa	PROPN
ajlm-269	33	40	.	.	PUNCT
ajlm-269	34	1	this	this	PRON
ajlm-269	34	2	is	be	AUX
ajlm-269	34	3	a	a	DET
ajlm-269	34	4	reference	reference	NOUN
ajlm-269	34	5	laboratory	laboratory	NOUN
ajlm-269	34	6	which	which	PRON
ajlm-269	34	7	receives	receive	VERB
ajlm-269	34	8	all	all	DET
ajlm-269	34	9	dbs	dbs	NOUN
ajlm-269	34	10	specimens	specimen	NOUN
ajlm-269	34	11	from	from	ADP
ajlm-269	34	12	public	public	ADJ
ajlm-269	34	13	sector	sector	NOUN
ajlm-269	34	14	facilities	facility	NOUN
ajlm-269	34	15	in	in	ADP
ajlm-269	34	16	kwazulu	kwazulu	NOUN
ajlm-269	34	17	-	-	PUNCT
ajlm-269	34	18	natal	natal	NOUN
ajlm-269	34	19	for	for	ADP
ajlm-269	34	20	pcr	pcr	NOUN
ajlm-269	34	21	testing	testing	NOUN
ajlm-269	34	22	of	of	ADP
ajlm-269	34	23	dna	dna	PROPN
ajlm-269	34	24	from	from	ADP
ajlm-269	34	25	hiv	hiv	PROPN
ajlm-269	34	26	-	-	PUNCT
ajlm-269	34	27	exposed	expose	VERB
ajlm-269	34	28	infants	infant	NOUN
ajlm-269	34	29	for	for	ADP
ajlm-269	34	30	early	early	ADJ
ajlm-269	34	31	diagnosis	diagnosis	NOUN
ajlm-269	34	32	of	of	ADP
ajlm-269	34	33	hiv	hiv	PROPN
ajlm-269	34	34	infection	infection	NOUN
ajlm-269	34	35	.	.	PUNCT
ajlm-269	35	1	the	the	DET
ajlm-269	35	2	number	number	NOUN
ajlm-269	35	3	of	of	ADP
ajlm-269	35	4	dbs	dbs	PROPN
ajlm-269	35	5	specimens	specimen	NOUN
ajlm-269	35	6	tested	test	VERB
ajlm-269	35	7	by	by	ADP
ajlm-269	35	8	the	the	DET
ajlm-269	35	9	laboratory	laboratory	NOUN
ajlm-269	35	10	has	have	AUX
ajlm-269	35	11	steadily	steadily	ADV
ajlm-269	35	12	increased	increase	VERB
ajlm-269	35	13	each	each	DET
ajlm-269	35	14	year	year	NOUN
ajlm-269	35	15	from	from	ADP
ajlm-269	35	16	13	13	NUM
ajlm-269	35	17	699	699	NUM
ajlm-269	35	18	in	in	ADP
ajlm-269	35	19	2005	2005	NUM
ajlm-269	35	20	to	to	ADP
ajlm-269	35	21	73	73	NUM
ajlm-269	35	22	033	033	NUM
ajlm-269	35	23	in	in	ADP
ajlm-269	35	24	2012	2012	NUM
ajlm-269	35	25	.	.	PUNCT
ajlm-269	36	1	the	the	DET
ajlm-269	36	2	uptake	uptake	NOUN
ajlm-269	36	3	of	of	ADP
ajlm-269	36	4	hiv	hiv	PROPN
ajlm-269	36	5	pcr	pcr	PROPN
ajlm-269	36	6	testing	testing	NOUN
ajlm-269	36	7	amongst	amongst	ADP
ajlm-269	36	8	hiv	hiv	PROPN
ajlm-269	36	9	-	-	PUNCT
ajlm-269	36	10	exposed	expose	VERB
ajlm-269	36	11	infants	infant	NOUN
ajlm-269	36	12	in	in	ADP
ajlm-269	36	13	kwazulu	kwazulu	PROPN
ajlm-269	36	14	-	-	PUNCT
ajlm-269	36	15	natal	natal	NOUN
ajlm-269	36	16	has	have	AUX
ajlm-269	36	17	also	also	ADV
ajlm-269	36	18	increased	increase	VERB
ajlm-269	36	19	from	from	ADP
ajlm-269	36	20	18	18	NUM
ajlm-269	36	21	%	%	NOUN
ajlm-269	36	22	in	in	ADP
ajlm-269	36	23	2005	2005	NUM
ajlm-269	36	24	to	to	ADP
ajlm-269	36	25	97.4	97.4	NUM
ajlm-269	36	26	%	%	NOUN
ajlm-269	36	27	in	in	ADP
ajlm-269	36	28	2011.30	2011.30	NUM
ajlm-269	36	29	a	a	DET
ajlm-269	36	30	sample	sample	NOUN
ajlm-269	36	31	size	size	NOUN
ajlm-269	36	32	of	of	ADP
ajlm-269	36	33	179	179	NUM
ajlm-269	36	34	was	be	AUX
ajlm-269	36	35	calculated	calculate	VERB
ajlm-269	36	36	using	use	VERB
ajlm-269	36	37	epi	epi	NOUN
ajlm-269	36	38	-	-	NOUN
ajlm-269	36	39	info	info	NOUN
ajlm-269	36	40	™	™	ADJ
ajlm-269	36	41	6	6	NUM
ajlm-269	36	42	(	(	PUNCT
ajlm-269	36	43	centre	centre	NOUN
ajlm-269	36	44	for	for	ADP
ajlm-269	36	45	disease	disease	NOUN
ajlm-269	36	46	control	control	NOUN
ajlm-269	36	47	,	,	PUNCT
ajlm-269	36	48	atlanta	atlanta	PROPN
ajlm-269	36	49	,	,	PUNCT
ajlm-269	36	50	georgia	georgia	PROPN
ajlm-269	36	51	,	,	PUNCT
ajlm-269	36	52	united	united	PROPN
ajlm-269	36	53	states	states	PROPN
ajlm-269	36	54	,	,	PUNCT
ajlm-269	36	55	1998	1998	NUM
ajlm-269	36	56	)	)	PUNCT
ajlm-269	36	57	using	use	VERB
ajlm-269	36	58	the	the	DET
ajlm-269	36	59	number	number	NOUN
ajlm-269	36	60	of	of	ADP
ajlm-269	36	61	live	live	ADJ
ajlm-269	36	62	births	birth	NOUN
ajlm-269	36	63	in	in	ADP
ajlm-269	36	64	kwazulu	kwazulu	PROPN
ajlm-269	36	65	-	-	PUNCT
ajlm-269	36	66	natal,30	natal,30	NOUN
ajlm-269	36	67	the	the	DET
ajlm-269	36	68	prevalence	prevalence	NOUN
ajlm-269	36	69	of	of	ADP
ajlm-269	36	70	hiv	hiv	PROPN
ajlm-269	36	71	amongst	amongst	ADP
ajlm-269	36	72	antenatal	antenatal	ADJ
ajlm-269	36	73	attendees	attendee	NOUN
ajlm-269	36	74	(	(	PUNCT
ajlm-269	36	75	which	which	PRON
ajlm-269	36	76	approximates	approximate	VERB
ajlm-269	36	77	the	the	DET
ajlm-269	36	78	percentage	percentage	NOUN
ajlm-269	36	79	of	of	ADP
ajlm-269	36	80	hiv	hiv	PROPN
ajlm-269	36	81	-	-	PUNCT
ajlm-269	36	82	exposed	expose	VERB
ajlm-269	36	83	infants)8	infants)8	NOUN
ajlm-269	36	84	and	and	CCONJ
ajlm-269	36	85	the	the	DET
ajlm-269	36	86	approximate	approximate	ADJ
ajlm-269	36	87	hcv	hcv	PROPN
ajlm-269	36	88	seropositivity	seropositivity	NOUN
ajlm-269	36	89	in	in	ADP
ajlm-269	36	90	infants	infant	NOUN
ajlm-269	36	91	,	,	PUNCT
ajlm-269	36	92	based	base	VERB
ajlm-269	36	93	on	on	ADP
ajlm-269	36	94	data	datum	NOUN
ajlm-269	36	95	from	from	ADP
ajlm-269	36	96	the	the	DET
ajlm-269	36	97	laboratory	laboratory	NOUN
ajlm-269	36	98	information	information	NOUN
ajlm-269	36	99	system	system	NOUN
ajlm-269	36	100	.	.	PUNCT
ajlm-269	37	1	the	the	DET
ajlm-269	37	2	sample	sample	NOUN
ajlm-269	37	3	size	size	NOUN
ajlm-269	37	4	was	be	AUX
ajlm-269	37	5	representative	representative	NOUN
ajlm-269	37	6	of	of	ADP
ajlm-269	37	7	hiv	hiv	PROPN
ajlm-269	37	8	-	-	PUNCT
ajlm-269	37	9	exposed	expose	VERB
ajlm-269	37	10	infants	infant	NOUN
ajlm-269	37	11	who	who	PRON
ajlm-269	37	12	attended	attend	VERB
ajlm-269	37	13	public	public	ADJ
ajlm-269	37	14	health	health	NOUN
ajlm-269	37	15	facilities	facility	NOUN
ajlm-269	37	16	in	in	ADP
ajlm-269	37	17	kwazulu	kwazulu	PROPN
ajlm-269	37	18	-	-	PUNCT
ajlm-269	37	19	natal	natal	NOUN
ajlm-269	37	20	and	and	CCONJ
ajlm-269	37	21	who	who	PRON
ajlm-269	37	22	required	require	VERB
ajlm-269	37	23	hiv	hiv	PROPN
ajlm-269	37	24	pcr	pcr	PROPN
ajlm-269	37	25	testing	testing	NOUN
ajlm-269	37	26	because	because	SCONJ
ajlm-269	37	27	of	of	ADP
ajlm-269	37	28	suspected	suspect	VERB
ajlm-269	37	29	hiv	hiv	PROPN
ajlm-269	37	30	exposure	exposure	NOUN
ajlm-269	37	31	or	or	CCONJ
ajlm-269	37	32	clinical	clinical	ADJ
ajlm-269	37	33	suspicion	suspicion	NOUN
ajlm-269	37	34	of	of	ADP
ajlm-269	37	35	hiv	hiv	PROPN
ajlm-269	37	36	infection	infection	NOUN
ajlm-269	37	37	.	.	PUNCT
ajlm-269	38	1	specimen	speciman	NOUN
ajlm-269	38	2	collection	collection	NOUN
ajlm-269	38	3	as	as	ADP
ajlm-269	38	4	part	part	NOUN
ajlm-269	38	5	of	of	ADP
ajlm-269	38	6	routine	routine	ADJ
ajlm-269	38	7	early	early	ADJ
ajlm-269	38	8	infant	infant	NOUN
ajlm-269	38	9	diagnosis	diagnosis	NOUN
ajlm-269	38	10	of	of	ADP
ajlm-269	38	11	hiv	hiv	PROPN
ajlm-269	38	12	,	,	PUNCT
ajlm-269	38	13	infants	infant	NOUN
ajlm-269	38	14	’	’	PART
ajlm-269	38	15	blood	blood	NOUN
ajlm-269	38	16	is	be	AUX
ajlm-269	38	17	collected	collect	VERB
ajlm-269	38	18	by	by	ADP
ajlm-269	38	19	heel	heel	NOUN
ajlm-269	38	20	-	-	PUNCT
ajlm-269	38	21	prick	prick	NOUN
ajlm-269	38	22	and	and	CCONJ
ajlm-269	38	23	spotted	spot	VERB
ajlm-269	38	24	onto	onto	ADP
ajlm-269	38	25	whatman	whatman	NOUN
ajlm-269	38	26	filter	filter	NOUN
ajlm-269	38	27	paper	paper	NOUN
ajlm-269	38	28	in	in	ADP
ajlm-269	38	29	four	four	NUM
ajlm-269	38	30	circles	circle	NOUN
ajlm-269	38	31	.	.	PUNCT
ajlm-269	39	1	in	in	ADP
ajlm-269	39	2	the	the	DET
ajlm-269	39	3	laboratory	laboratory	NOUN
ajlm-269	39	4	,	,	PUNCT
ajlm-269	39	5	two	two	NUM
ajlm-269	39	6	spots	spot	NOUN
ajlm-269	39	7	are	be	AUX
ajlm-269	39	8	cut	cut	VERB
ajlm-269	39	9	out	out	ADP
ajlm-269	39	10	and	and	CCONJ
ajlm-269	39	11	used	use	VERB
ajlm-269	39	12	for	for	ADP
ajlm-269	39	13	routine	routine	ADJ
ajlm-269	39	14	hiv	hiv	PROPN
ajlm-269	39	15	pcr	pcr	PROPN
ajlm-269	39	16	testing	testing	NOUN
ajlm-269	39	17	within	within	ADP
ajlm-269	39	18	four	four	NUM
ajlm-269	39	19	to	to	PART
ajlm-269	39	20	five	five	NUM
ajlm-269	39	21	days	day	NOUN
ajlm-269	39	22	of	of	ADP
ajlm-269	39	23	dbs	dbs	PROPN
ajlm-269	39	24	specimen	speciman	NOUN
ajlm-269	39	25	collection	collection	NOUN
ajlm-269	39	26	.	.	PUNCT
ajlm-269	40	1	after	after	ADP
ajlm-269	40	2	releasing	release	VERB
ajlm-269	40	3	the	the	DET
ajlm-269	40	4	results	result	NOUN
ajlm-269	40	5	,	,	PUNCT
ajlm-269	40	6	the	the	DET
ajlm-269	40	7	card	card	NOUN
ajlm-269	40	8	(	(	PUNCT
ajlm-269	40	9	with	with	ADP
ajlm-269	40	10	the	the	DET
ajlm-269	40	11	two	two	NUM
ajlm-269	40	12	unused	unused	ADJ
ajlm-269	40	13	spots	spot	NOUN
ajlm-269	40	14	)	)	PUNCT
ajlm-269	40	15	is	be	AUX
ajlm-269	40	16	routinely	routinely	ADV
ajlm-269	40	17	stored	store	VERB
ajlm-269	40	18	at	at	ADP
ajlm-269	40	19	room	room	NOUN
ajlm-269	40	20	temperature	temperature	NOUN
ajlm-269	40	21	for	for	ADP
ajlm-269	40	22	at	at	ADV
ajlm-269	40	23	least	least	ADV
ajlm-269	40	24	three	three	NUM
ajlm-269	40	25	months	month	NOUN
ajlm-269	40	26	and	and	CCONJ
ajlm-269	40	27	then	then	ADV
ajlm-269	40	28	discarded	discard	VERB
ajlm-269	40	29	.	.	PUNCT
ajlm-269	41	1	these	these	DET
ajlm-269	41	2	discarded	discard	VERB
ajlm-269	41	3	dbs	dbs	NOUN
ajlm-269	41	4	specimens	specimen	NOUN
ajlm-269	41	5	were	be	AUX
ajlm-269	41	6	tested	test	VERB
ajlm-269	41	7	for	for	ADP
ajlm-269	41	8	hcv	hcv	PROPN
ajlm-269	41	9	after	after	SCONJ
ajlm-269	41	10	specimens	specimen	NOUN
ajlm-269	41	11	had	have	AUX
ajlm-269	41	12	been	be	AUX
ajlm-269	41	13	stored	store	VERB
ajlm-269	41	14	for	for	ADP
ajlm-269	41	15	at	at	ADV
ajlm-269	41	16	least	least	ADV
ajlm-269	41	17	three	three	NUM
ajlm-269	41	18	months	month	NOUN
ajlm-269	41	19	after	after	ADP
ajlm-269	41	20	initial	initial	ADJ
ajlm-269	41	21	collection	collection	NOUN
ajlm-269	41	22	.	.	PUNCT
ajlm-269	42	1	specimens	specimen	NOUN
ajlm-269	42	2	with	with	ADP
ajlm-269	42	3	inadequate	inadequate	ADJ
ajlm-269	42	4	volume	volume	NOUN
ajlm-269	42	5	(	(	PUNCT
ajlm-269	42	6	where	where	SCONJ
ajlm-269	42	7	the	the	DET
ajlm-269	42	8	entire	entire	ADJ
ajlm-269	42	9	circle	circle	NOUN
ajlm-269	42	10	was	be	AUX
ajlm-269	42	11	not	not	PART
ajlm-269	42	12	filled	fill	VERB
ajlm-269	42	13	with	with	ADP
ajlm-269	42	14	blood	blood	NOUN
ajlm-269	42	15	)	)	PUNCT
ajlm-269	42	16	were	be	AUX
ajlm-269	42	17	excluded	exclude	VERB
ajlm-269	42	18	from	from	ADP
ajlm-269	42	19	the	the	DET
ajlm-269	42	20	study	study	NOUN
ajlm-269	42	21	.	.	PUNCT
ajlm-269	43	1	acceptable	acceptable	ADJ
ajlm-269	43	2	dbs	dbs	PROPN
ajlm-269	43	3	specimens	specimen	NOUN
ajlm-269	43	4	were	be	AUX
ajlm-269	43	5	sequentially	sequentially	ADV
ajlm-269	43	6	selected	select	VERB
ajlm-269	43	7	from	from	ADP
ajlm-269	43	8	august	august	PROPN
ajlm-269	43	9	to	to	ADP
ajlm-269	43	10	december	december	PROPN
ajlm-269	43	11	2010	2010	NUM
ajlm-269	43	12	until	until	SCONJ
ajlm-269	43	13	179	179	NUM
ajlm-269	43	14	specimens	specimen	NOUN
ajlm-269	43	15	were	be	AUX
ajlm-269	43	16	obtained	obtain	VERB
ajlm-269	43	17	.	.	PUNCT
ajlm-269	44	1	external	external	ADJ
ajlm-269	44	2	quality	quality	NOUN
ajlm-269	44	3	control	control	NOUN
ajlm-269	44	4	specimens	specimen	NOUN
ajlm-269	44	5	were	be	AUX
ajlm-269	44	6	purchased	purchase	VERB
ajlm-269	44	7	from	from	ADP
ajlm-269	44	8	quality	quality	NOUN
ajlm-269	44	9	control	control	NOUN
ajlm-269	44	10	for	for	ADP
ajlm-269	44	11	molecular	molecular	ADJ
ajlm-269	44	12	diagnostics	diagnostic	NOUN
ajlm-269	44	13	(	(	PUNCT
ajlm-269	44	14	qcmd	qcmd	NUM
ajlm-269	44	15	;	;	PUNCT
ajlm-269	44	16	glasgow	glasgow	PROPN
ajlm-269	44	17	,	,	PUNCT
ajlm-269	44	18	scotland	scotland	PROPN
ajlm-269	44	19	)	)	PUNCT
ajlm-269	44	20	and	and	CCONJ
ajlm-269	44	21	internal	internal	ADJ
ajlm-269	44	22	quality	quality	NOUN
ajlm-269	44	23	control	control	NOUN
ajlm-269	44	24	specimens	specimen	NOUN
ajlm-269	44	25	were	be	AUX
ajlm-269	44	26	prepared	prepare	VERB
ajlm-269	44	27	in	in	ADP
ajlm-269	44	28	-	-	PUNCT
ajlm-269	44	29	house	house	NOUN
ajlm-269	44	30	.	.	PUNCT
ajlm-269	45	1	laboratory	laboratory	NOUN
ajlm-269	45	2	methods	method	NOUN
ajlm-269	45	3	before	before	ADP
ajlm-269	45	4	automated	automate	VERB
ajlm-269	45	5	extraction	extraction	NOUN
ajlm-269	45	6	,	,	PUNCT
ajlm-269	45	7	using	use	VERB
ajlm-269	45	8	the	the	DET
ajlm-269	45	9	nuclisens	nuclisen	NOUN
ajlm-269	45	10	®	®	NOUN
ajlm-269	45	11	easymag	easymag	NOUN
ajlm-269	45	12	®	®	NOUN
ajlm-269	45	13	extraction	extraction	NOUN
ajlm-269	45	14	(	(	PUNCT
ajlm-269	45	15	biomérieux	biomérieux	PROPN
ajlm-269	45	16	,	,	PUNCT
ajlm-269	45	17	marcy	marcy	PROPN
ajlm-269	45	18	i’etoile	i’etoile	PROPN
ajlm-269	45	19	,	,	PUNCT
ajlm-269	45	20	france	france	PROPN
ajlm-269	45	21	)	)	PUNCT
ajlm-269	45	22	,	,	PUNCT
ajlm-269	45	23	two	two	NUM
ajlm-269	45	24	dbs	dbs	NOUN
ajlm-269	45	25	(	(	PUNCT
ajlm-269	45	26	approximately	approximately	ADV
ajlm-269	45	27	50	50	NUM
ajlm-269	45	28	µl	µl	ADP
ajlm-269	45	29	blood	blood	NOUN
ajlm-269	45	30	per	per	ADP
ajlm-269	45	31	spot	spot	NOUN
ajlm-269	45	32	)	)	PUNCT
ajlm-269	45	33	were	be	AUX
ajlm-269	45	34	cut	cut	VERB
ajlm-269	45	35	with	with	ADP
ajlm-269	45	36	sterilised	sterilise	VERB
ajlm-269	45	37	scissors	scissor	NOUN
ajlm-269	45	38	into	into	ADP
ajlm-269	45	39	a	a	DET
ajlm-269	45	40	1.5	1.5	NUM
ajlm-269	45	41	ml	ml	NOUN
ajlm-269	45	42	eppendorf	eppendorf	PROPN
ajlm-269	45	43	tube	tube	NOUN
ajlm-269	45	44	and	and	CCONJ
ajlm-269	45	45	2	2	NUM
ajlm-269	45	46	ml	ml	NOUN
ajlm-269	45	47	nuclisens	nuclisen	NOUN
ajlm-269	45	48	®	®	NOUN
ajlm-269	45	49	easymag	easymag	NOUN
ajlm-269	45	50	®	®	NOUN
ajlm-269	45	51	lysis	lysis	NOUN
ajlm-269	45	52	buffer	buffer	NOUN
ajlm-269	45	53	containing	contain	VERB
ajlm-269	45	54	5	5	NUM
ajlm-269	45	55	 	 	SPACE
ajlm-269	45	56	m	m	NOUN
ajlm-269	45	57	guanidinium	guanidinium	NOUN
ajlm-269	45	58	thiocyanate	thiocyanate	NOUN
ajlm-269	45	59	was	be	AUX
ajlm-269	45	60	added	add	VERB
ajlm-269	45	61	to	to	ADP
ajlm-269	45	62	the	the	DET
ajlm-269	45	63	tube	tube	NOUN
ajlm-269	45	64	.	.	PUNCT
ajlm-269	46	1	the	the	DET
ajlm-269	46	2	lysis	lysis	NOUN
ajlm-269	46	3	buffer	buffer	NOUN
ajlm-269	46	4	was	be	AUX
ajlm-269	46	5	also	also	ADV
ajlm-269	46	6	added	add	VERB
ajlm-269	46	7	to	to	ADP
ajlm-269	46	8	the	the	DET
ajlm-269	46	9	quality	quality	NOUN
ajlm-269	46	10	control	control	NOUN
ajlm-269	46	11	specimens	specimen	NOUN
ajlm-269	46	12	.	.	PUNCT
ajlm-269	47	1	the	the	DET
ajlm-269	47	2	specimens	specimen	NOUN
ajlm-269	47	3	were	be	AUX
ajlm-269	47	4	agitated	agitate	VERB
ajlm-269	47	5	for	for	ADP
ajlm-269	47	6	30	30	NUM
ajlm-269	47	7	minutes	minute	NOUN
ajlm-269	47	8	on	on	ADP
ajlm-269	47	9	an	an	DET
ajlm-269	47	10	orbital	orbital	ADJ
ajlm-269	47	11	shaker	shaker	NOUN
ajlm-269	47	12	and	and	CCONJ
ajlm-269	47	13	centrifuged	centrifuge	VERB
ajlm-269	47	14	at	at	ADP
ajlm-269	47	15	1500	1500	NUM
ajlm-269	47	16	x	x	SYM
ajlm-269	47	17	g	g	NOUN
ajlm-269	47	18	for	for	ADP
ajlm-269	47	19	two	two	NUM
ajlm-269	47	20	minutes	minute	NOUN
ajlm-269	47	21	.	.	PUNCT
ajlm-269	48	1	supernatant	supernatant	NOUN
ajlm-269	48	2	was	be	AUX
ajlm-269	48	3	pipetted	pipette	VERB
ajlm-269	48	4	into	into	ADP
ajlm-269	48	5	transfer	transfer	NOUN
ajlm-269	48	6	wells	well	NOUN
ajlm-269	48	7	,	,	PUNCT
ajlm-269	48	8	which	which	PRON
ajlm-269	48	9	were	be	AUX
ajlm-269	48	10	then	then	ADV
ajlm-269	48	11	loaded	load	VERB
ajlm-269	48	12	onto	onto	ADP
ajlm-269	48	13	the	the	DET
ajlm-269	48	14	automated	automate	VERB
ajlm-269	48	15	extraction	extraction	NOUN
ajlm-269	48	16	system	system	NOUN
ajlm-269	48	17	.	.	PUNCT
ajlm-269	49	1	briefly	briefly	ADV
ajlm-269	49	2	,	,	PUNCT
ajlm-269	49	3	cell	cell	NOUN
ajlm-269	49	4	-	-	PUNCT
ajlm-269	49	5	bound	bind	VERB
ajlm-269	49	6	nucleic	nucleic	ADJ
ajlm-269	49	7	acids	acid	NOUN
ajlm-269	49	8	were	be	AUX
ajlm-269	49	9	released	release	VERB
ajlm-269	49	10	with	with	ADP
ajlm-269	49	11	the	the	DET
ajlm-269	49	12	chaotropic	chaotropic	NOUN
ajlm-269	49	13	agent	agent	NOUN
ajlm-269	49	14	−	−	PROPN
ajlm-269	49	15	guanidinium	guanidinium	NOUN
ajlm-269	49	16	thiocyanate	thiocyanate	NOUN
ajlm-269	49	17	−	−	PROPN
ajlm-269	49	18	and	and	CCONJ
ajlm-269	49	19	then	then	ADV
ajlm-269	49	20	bound	bind	VERB
ajlm-269	49	21	to	to	PART
ajlm-269	49	22	silica	silica	VERB
ajlm-269	49	23	particles	particle	NOUN
ajlm-269	49	24	,	,	PUNCT
ajlm-269	49	25	which	which	PRON
ajlm-269	49	26	were	be	AUX
ajlm-269	49	27	immobilised	immobilise	VERB
ajlm-269	49	28	on	on	ADP
ajlm-269	49	29	a	a	DET
ajlm-269	49	30	filter	filter	NOUN
ajlm-269	49	31	.	.	PUNCT
ajlm-269	50	1	impurities	impurity	NOUN
ajlm-269	50	2	were	be	AUX
ajlm-269	50	3	removed	remove	VERB
ajlm-269	50	4	by	by	ADP
ajlm-269	50	5	several	several	ADJ
ajlm-269	50	6	washes	wash	NOUN
ajlm-269	50	7	in	in	ADP
ajlm-269	50	8	buffers	buffer	NOUN
ajlm-269	50	9	containing	contain	VERB
ajlm-269	50	10	guanidinium	guanidinium	NOUN
ajlm-269	50	11	thiocyanate	thiocyanate	NOUN
ajlm-269	50	12	,	,	PUNCT
ajlm-269	50	13	70	70	NUM
ajlm-269	50	14	%	%	NOUN
ajlm-269	50	15	ethanol	ethanol	NOUN
ajlm-269	50	16	and	and	CCONJ
ajlm-269	50	17	acetone	acetone	NOUN
ajlm-269	50	18	,	,	PUNCT
ajlm-269	50	19	after	after	ADP
ajlm-269	50	20	which	which	PRON
ajlm-269	50	21	the	the	DET
ajlm-269	50	22	purified	purified	ADJ
ajlm-269	50	23	nucleic	nucleic	ADJ
ajlm-269	50	24	acids	acid	NOUN
ajlm-269	50	25	were	be	AUX
ajlm-269	50	26	eluted	elute	VERB
ajlm-269	50	27	in	in	ADP
ajlm-269	50	28	molecular	molecular	ADJ
ajlm-269	50	29	grade	grade	NOUN
ajlm-269	50	30	water	water	NOUN
ajlm-269	50	31	.	.	PUNCT
ajlm-269	51	1	the	the	DET
ajlm-269	51	2	lightcycler	lightcycler	PROPN
ajlm-269	51	3	®	®	NOUN
ajlm-269	51	4	2.0	2.0	NUM
ajlm-269	51	5	instrument	instrument	NOUN
ajlm-269	51	6	(	(	PUNCT
ajlm-269	51	7	roche	roche	NOUN
ajlm-269	51	8	diagnostics	diagnostic	NOUN
ajlm-269	51	9	,	,	PUNCT
ajlm-269	51	10	mannheim	mannheim	PROPN
ajlm-269	51	11	,	,	PUNCT
ajlm-269	51	12	germany	germany	PROPN
ajlm-269	51	13	)	)	PUNCT
ajlm-269	51	14	was	be	AUX
ajlm-269	51	15	used	use	VERB
ajlm-269	51	16	to	to	PART
ajlm-269	51	17	perform	perform	VERB
ajlm-269	51	18	reverse	reverse	ADJ
ajlm-269	51	19	transcription	transcription	NOUN
ajlm-269	51	20	and	and	CCONJ
ajlm-269	51	21	amplification	amplification	NOUN
ajlm-269	51	22	in	in	ADP
ajlm-269	51	23	a	a	DET
ajlm-269	51	24	one	one	NUM
ajlm-269	51	25	-	-	PUNCT
ajlm-269	51	26	step	step	NOUN
ajlm-269	51	27	assay	assay	NOUN
ajlm-269	51	28	using	use	VERB
ajlm-269	51	29	the	the	DET
ajlm-269	51	30	lightcycler	lightcycler	ADJ
ajlm-269	51	31	®	®	PROPN
ajlm-269	51	32	rna	rna	NOUN
ajlm-269	51	33	master	master	NOUN
ajlm-269	51	34	sybr	sybr	PROPN
ajlm-269	51	35	green	green	PROPN
ajlm-269	51	36	i	i	PRON
ajlm-269	51	37	kit	kit	NOUN
ajlm-269	51	38	(	(	PUNCT
ajlm-269	51	39	roche	roche	NOUN
ajlm-269	51	40	diagnostics	diagnostic	NOUN
ajlm-269	51	41	,	,	PUNCT
ajlm-269	51	42	mannheim	mannheim	PROPN
ajlm-269	51	43	,	,	PUNCT
ajlm-269	51	44	germany	germany	PROPN
ajlm-269	51	45	)	)	PUNCT
ajlm-269	51	46	,	,	PUNCT
ajlm-269	51	47	which	which	PRON
ajlm-269	51	48	contained	contain	VERB
ajlm-269	51	49	the	the	DET
ajlm-269	51	50	enzymes	enzyme	NOUN
ajlm-269	51	51	thermus	thermus	PROPN
ajlm-269	51	52	thermophilus	thermophilus	PROPN
ajlm-269	51	53	dna	dna	PROPN
ajlm-269	51	54	polymerase	polymerase	NOUN
ajlm-269	51	55	,	,	PUNCT
ajlm-269	51	56	dntps	dntps	ADJ
ajlm-269	51	57	,	,	PUNCT
ajlm-269	51	58	reaction	reaction	NOUN
ajlm-269	51	59	buffer	buffer	NOUN
ajlm-269	51	60	and	and	CCONJ
ajlm-269	51	61	sybr	sybr	VERB
ajlm-269	51	62	green	green	ADJ
ajlm-269	51	63	i	i	PRON
ajlm-269	51	64	fluorescent	fluorescent	VERB
ajlm-269	51	65	dye	dye	NOUN
ajlm-269	51	66	.	.	PUNCT
ajlm-269	52	1	the	the	DET
ajlm-269	52	2	primers	primer	NOUN
ajlm-269	52	3	used	use	VERB
ajlm-269	52	4	in	in	ADP
ajlm-269	52	5	the	the	DET
ajlm-269	52	6	reverse	reverse	ADJ
ajlm-269	52	7	transcription	transcription	NOUN
ajlm-269	52	8	pcr	pcr	NOUN
ajlm-269	52	9	were	be	AUX
ajlm-269	52	10	ky80	ky80	PROPN
ajlm-269	52	11	(	(	PUNCT
ajlm-269	52	12	sense	sense	NOUN
ajlm-269	52	13	)	)	PUNCT
ajlm-269	52	14	5’-gca	5’-gca	NUM
ajlm-269	52	15	gaa	gaa	NOUN
ajlm-269	52	16	agc	agc	PROPN
ajlm-269	52	17	gtc	gtc	PROPN
ajlm-269	52	18	tag	tag	PROPN
ajlm-269	52	19	cca	cca	PROPN
ajlm-269	52	20	tgg	tgg	PROPN
ajlm-269	52	21	cgt-3	cgt-3	NOUN
ajlm-269	52	22	’	'	PUNCT
ajlm-269	52	23	and	and	CCONJ
ajlm-269	52	24	ky78	ky78	PROPN
ajlm-269	52	25	(	(	PUNCT
ajlm-269	52	26	antisense	antisense	NOUN
ajlm-269	52	27	)	)	PUNCT
ajlm-269	52	28	5’-ctc	5’-ctc	NUM
ajlm-269	52	29	gca	gca	NOUN
ajlm-269	52	30	agc	agc	PROPN
ajlm-269	52	31	acc	acc	PROPN
ajlm-269	52	32	cta	cta	PROPN
ajlm-269	52	33	tca	tca	PROPN
ajlm-269	52	34	ggc	ggc	PROPN
ajlm-269	52	35	agt-3	agt-3	NOUN
ajlm-269	52	36	’	'	PUNCT
ajlm-269	52	37	.	.	PUNCT
ajlm-269	53	1	these	these	DET
ajlm-269	53	2	primers	primer	NOUN
ajlm-269	53	3	target	target	VERB
ajlm-269	53	4	the	the	DET
ajlm-269	53	5	highly	highly	ADV
ajlm-269	53	6	-	-	PUNCT
ajlm-269	53	7	conserved	conserve	VERB
ajlm-269	53	8	5’-non	5’-non	NUM
ajlm-269	53	9	-	-	PUNCT
ajlm-269	53	10	coding	code	VERB
ajlm-269	53	11	region	region	NOUN
ajlm-269	53	12	of	of	ADP
ajlm-269	53	13	the	the	DET
ajlm-269	53	14	hcv	hcv	NOUN
ajlm-269	53	15	genome	genome	NOUN
ajlm-269	53	16	to	to	PART
ajlm-269	53	17	produce	produce	VERB
ajlm-269	53	18	a	a	DET
ajlm-269	53	19	pcr	pcr	ADJ
ajlm-269	53	20	product	product	NOUN
ajlm-269	53	21	of	of	ADP
ajlm-269	53	22	244	244	NUM
ajlm-269	53	23	bp	bp	PROPN
ajlm-269	53	24	and	and	CCONJ
ajlm-269	53	25	are	be	AUX
ajlm-269	53	26	known	know	VERB
ajlm-269	53	27	to	to	PART
ajlm-269	53	28	detect	detect	VERB
ajlm-269	53	29	all	all	DET
ajlm-269	53	30	hcv	hcv	NOUN
ajlm-269	53	31	genotypes.31	genotypes.31	VERB
ajlm-269	53	32	the	the	DET
ajlm-269	53	33	reverse	reverse	ADJ
ajlm-269	53	34	transcription	transcription	NOUN
ajlm-269	53	35	pcr	pcr	NOUN
ajlm-269	53	36	master	master	NOUN
ajlm-269	53	37	mix	mix	NOUN
ajlm-269	53	38	consisted	consist	VERB
ajlm-269	53	39	of	of	ADP
ajlm-269	53	40	pcr	pcr	ADJ
ajlm-269	53	41	-	-	PUNCT
ajlm-269	53	42	grade	grade	NOUN
ajlm-269	53	43	water	water	NOUN
ajlm-269	53	44	,	,	PUNCT
ajlm-269	53	45	mn(oac)2	mn(oac)2	NOUN
ajlm-269	53	46	,	,	PUNCT
ajlm-269	53	47	both	both	DET
ajlm-269	53	48	primers	primer	NOUN
ajlm-269	53	49	and	and	CCONJ
ajlm-269	53	50	the	the	DET
ajlm-269	53	51	lightcycler	lightcycler	PROPN
ajlm-269	53	52	®	®	PROPN
ajlm-269	53	53	rna	rna	NOUN
ajlm-269	53	54	master	master	NOUN
ajlm-269	53	55	sybr	sybr	PROPN
ajlm-269	53	56	green	green	PROPN
ajlm-269	53	57	i	i	PROPN
ajlm-269	53	58	mix	mix	NOUN
ajlm-269	53	59	.	.	PUNCT
ajlm-269	54	1	the	the	DET
ajlm-269	54	2	reverse	reverse	ADJ
ajlm-269	54	3	transcription	transcription	NOUN
ajlm-269	54	4	and	and	CCONJ
ajlm-269	54	5	pcr	pcr	NOUN
ajlm-269	54	6	parameters	parameter	NOUN
ajlm-269	54	7	were	be	AUX
ajlm-269	54	8	set	set	VERB
ajlm-269	54	9	according	accord	VERB
ajlm-269	54	10	to	to	ADP
ajlm-269	54	11	the	the	DET
ajlm-269	54	12	lightcycler	lightcycler	NOUN
ajlm-269	54	13	®	®	PROPN
ajlm-269	54	14	rna	rna	NOUN
ajlm-269	54	15	master	master	NOUN
ajlm-269	54	16	sybr	sybr	PROPN
ajlm-269	54	17	green	green	PROPN
ajlm-269	54	18	i	i	PRON
ajlm-269	54	19	package	package	NOUN
ajlm-269	54	20	insert	insert	NOUN
ajlm-269	54	21	.	.	PUNCT
ajlm-269	55	1	reverse	reverse	ADJ
ajlm-269	55	2	transcription	transcription	NOUN
ajlm-269	55	3	of	of	ADP
ajlm-269	55	4	the	the	DET
ajlm-269	55	5	template	template	NOUN
ajlm-269	55	6	rna	rna	NOUN
ajlm-269	55	7	was	be	AUX
ajlm-269	55	8	performed	perform	VERB
ajlm-269	55	9	at	at	ADP
ajlm-269	55	10	61	61	NUM
ajlm-269	55	11	°	°	ADP
ajlm-269	55	12	c	c	NOUN
ajlm-269	55	13	for	for	ADP
ajlm-269	55	14	20	20	NUM
ajlm-269	55	15	minutes	minute	NOUN
ajlm-269	55	16	.	.	PUNCT
ajlm-269	56	1	complementary	complementary	ADJ
ajlm-269	56	2	dna	dna	PROPN
ajlm-269	56	3	(	(	PUNCT
ajlm-269	56	4	cdna	cdna	PROPN
ajlm-269	56	5	)	)	PUNCT
ajlm-269	56	6	was	be	AUX
ajlm-269	56	7	amplified	amplify	VERB
ajlm-269	56	8	over	over	ADP
ajlm-269	56	9	40	40	NUM
ajlm-269	56	10	cycles	cycle	NOUN
ajlm-269	56	11	of	of	ADP
ajlm-269	56	12	denaturation	denaturation	NOUN
ajlm-269	56	13	,	,	PUNCT
ajlm-269	56	14	annealing	annealing	NOUN
ajlm-269	56	15	and	and	CCONJ
ajlm-269	56	16	extension	extension	NOUN
ajlm-269	56	17	.	.	PUNCT
ajlm-269	57	1	denaturation	denaturation	NOUN
ajlm-269	57	2	and	and	CCONJ
ajlm-269	57	3	extension	extension	NOUN
ajlm-269	57	4	were	be	AUX
ajlm-269	57	5	performed	perform	VERB
ajlm-269	57	6	at	at	ADP
ajlm-269	57	7	the	the	DET
ajlm-269	57	8	temperatures	temperature	NOUN
ajlm-269	57	9	on	on	ADP
ajlm-269	57	10	the	the	DET
ajlm-269	57	11	package	package	NOUN
ajlm-269	57	12	insert	insert	NOUN
ajlm-269	57	13	.	.	PUNCT
ajlm-269	58	1	the	the	DET
ajlm-269	58	2	annealing	anneal	VERB
ajlm-269	58	3	temperature	temperature	NOUN
ajlm-269	58	4	was	be	AUX
ajlm-269	58	5	calculated	calculate	VERB
ajlm-269	58	6	using	use	VERB
ajlm-269	58	7	the	the	DET
ajlm-269	58	8	melting	melting	NOUN
ajlm-269	58	9	temperatures	temperature	NOUN
ajlm-269	58	10	of	of	ADP
ajlm-269	58	11	the	the	DET
ajlm-269	58	12	primers	primer	NOUN
ajlm-269	58	13	,	,	PUNCT
ajlm-269	58	14	namely	namely	ADV
ajlm-269	58	15	,	,	PUNCT
ajlm-269	58	16	65	65	NUM
ajlm-269	58	17	°	°	NOUN
ajlm-269	58	18	c	c	X
ajlm-269	58	19	(	(	PUNCT
ajlm-269	58	20	4	4	NUM
ajlm-269	58	21	°	°	PRON
ajlm-269	58	22	c	c	NOUN
ajlm-269	58	23	subtracted	subtract	VERB
ajlm-269	58	24	from	from	ADP
ajlm-269	58	25	69	69	NUM
ajlm-269	58	26	°	°	NOUN
ajlm-269	58	27	c	c	NOUN
ajlm-269	58	28	)	)	PUNCT
ajlm-269	58	29	,	,	PUNCT
ajlm-269	58	30	using	use	VERB
ajlm-269	58	31	standard	standard	ADJ
ajlm-269	58	32	pcr	pcr	ADJ
ajlm-269	58	33	optimisation	optimisation	NOUN
ajlm-269	58	34	guidelines	guideline	NOUN
ajlm-269	58	35	,	,	PUNCT
ajlm-269	58	36	whereby	whereby	SCONJ
ajlm-269	58	37	a	a	DET
ajlm-269	58	38	range	range	NOUN
ajlm-269	58	39	of	of	ADP
ajlm-269	58	40	annealing	anneal	VERB
ajlm-269	58	41	temperatures	temperature	NOUN
ajlm-269	58	42	(	(	PUNCT
ajlm-269	58	43	55	55	NUM
ajlm-269	58	44	°	°	NOUN
ajlm-269	58	45	c	c	NOUN
ajlm-269	58	46	to	to	ADP
ajlm-269	58	47	65	65	NUM
ajlm-269	58	48	°	°	NOUN
ajlm-269	58	49	c	c	NOUN
ajlm-269	58	50	)	)	PUNCT
ajlm-269	58	51	was	be	AUX
ajlm-269	58	52	used	use	VERB
ajlm-269	58	53	to	to	PART
ajlm-269	58	54	determine	determine	VERB
ajlm-269	58	55	the	the	DET
ajlm-269	58	56	optimal	optimal	ADJ
ajlm-269	58	57	annealing	annealing	NOUN
ajlm-269	58	58	temperature	temperature	NOUN
ajlm-269	58	59	,	,	PUNCT
ajlm-269	58	60	where	where	SCONJ
ajlm-269	58	61	efficiency	efficiency	NOUN
ajlm-269	58	62	of	of	ADP
ajlm-269	58	63	pcr	pcr	ADJ
ajlm-269	58	64	amplification	amplification	NOUN
ajlm-269	58	65	was	be	AUX
ajlm-269	58	66	maximal.32	maximal.32	VERB
ajlm-269	58	67	melting	melting	NOUN
ajlm-269	58	68	curve	curve	NOUN
ajlm-269	58	69	analysis	analysis	NOUN
ajlm-269	58	70	to	to	PART
ajlm-269	58	71	identify	identify	VERB
ajlm-269	58	72	pcr	pcr	ADJ
ajlm-269	58	73	products	product	NOUN
ajlm-269	58	74	was	be	AUX
ajlm-269	58	75	performed	perform	VERB
ajlm-269	58	76	as	as	SCONJ
ajlm-269	58	77	described	describe	VERB
ajlm-269	58	78	on	on	ADP
ajlm-269	58	79	the	the	DET
ajlm-269	58	80	package	package	NOUN
ajlm-269	58	81	insert	insert	NOUN
ajlm-269	58	82	.	.	PUNCT
ajlm-269	59	1	the	the	DET
ajlm-269	59	2	pcr	pcr	PROPN
ajlm-269	59	3	products	product	NOUN
ajlm-269	59	4	were	be	AUX
ajlm-269	59	5	detected	detect	VERB
ajlm-269	59	6	with	with	ADP
ajlm-269	59	7	a	a	DET
ajlm-269	59	8	sybr	sybr	NOUN
ajlm-269	59	9	green	green	NOUN
ajlm-269	59	10	i	i	PRON
ajlm-269	59	11	dye	dye	VERB
ajlm-269	59	12	,	,	PUNCT
ajlm-269	59	13	which	which	PRON
ajlm-269	59	14	intercalates	intercalate	VERB
ajlm-269	59	15	with	with	ADP
ajlm-269	59	16	double	double	ADV
ajlm-269	59	17	-	-	PUNCT
ajlm-269	59	18	stranded	strand	VERB
ajlm-269	59	19	dna	dna	NOUN
ajlm-269	59	20	and	and	CCONJ
ajlm-269	59	21	is	be	AUX
ajlm-269	59	22	detected	detect	VERB
ajlm-269	59	23	at	at	ADP
ajlm-269	59	24	a	a	DET
ajlm-269	59	25	wavelength	wavelength	NOUN
ajlm-269	59	26	of	of	ADP
ajlm-269	59	27	530	530	NUM
ajlm-269	59	28	nm	nm	NOUN
ajlm-269	59	29	.	.	PUNCT
ajlm-269	60	1	primer	primer	NOUN
ajlm-269	60	2	concentrations	concentration	NOUN
ajlm-269	60	3	and	and	CCONJ
ajlm-269	60	4	template	template	NOUN
ajlm-269	60	5	volumes	volume	NOUN
ajlm-269	60	6	were	be	AUX
ajlm-269	60	7	optimised	optimise	VERB
ajlm-269	60	8	using	use	VERB
ajlm-269	60	9	the	the	DET
ajlm-269	60	10	methods	method	NOUN
ajlm-269	60	11	described	describe	VERB
ajlm-269	60	12	on	on	ADP
ajlm-269	60	13	the	the	DET
ajlm-269	60	14	lightcycler	lightcycler	PROPN
ajlm-269	60	15	®	®	PROPN
ajlm-269	60	16	rna	rna	NOUN
ajlm-269	60	17	master	master	NOUN
ajlm-269	60	18	sybr	sybr	PROPN
ajlm-269	60	19	green	green	PROPN
ajlm-269	60	20	i	i	PRON
ajlm-269	60	21	package	package	NOUN
ajlm-269	60	22	insert	insert	PROPN
ajlm-269	60	23	.	.	PUNCT
ajlm-269	61	1	primer	primer	NOUN
ajlm-269	61	2	concentrations	concentration	NOUN
ajlm-269	61	3	ranging	range	VERB
ajlm-269	61	4	from	from	ADP
ajlm-269	61	5	0.1	0.1	NUM
ajlm-269	61	6	µm	µm	NOUN
ajlm-269	61	7	to	to	ADP
ajlm-269	61	8	0.5	0.5	NUM
ajlm-269	61	9	µm	µm	NUM
ajlm-269	61	10	,	,	PUNCT
ajlm-269	61	11	using	use	VERB
ajlm-269	61	12	two	two	NUM
ajlm-269	61	13	-	-	PUNCT
ajlm-269	61	14	fold	fold	ADJ
ajlm-269	61	15	dilutions	dilution	NOUN
ajlm-269	61	16	,	,	PUNCT
ajlm-269	61	17	were	be	AUX
ajlm-269	61	18	used	use	VERB
ajlm-269	61	19	to	to	PART
ajlm-269	61	20	determine	determine	VERB
ajlm-269	61	21	the	the	DET
ajlm-269	61	22	optimal	optimal	ADJ
ajlm-269	61	23	primer	primer	NOUN
ajlm-269	61	24	concentration	concentration	NOUN
ajlm-269	61	25	capable	capable	ADJ
ajlm-269	61	26	of	of	ADP
ajlm-269	61	27	detecting	detect	VERB
ajlm-269	61	28	the	the	DET
ajlm-269	61	29	lowest	low	ADJ
ajlm-269	61	30	concentration	concentration	NOUN
ajlm-269	61	31	of	of	ADP
ajlm-269	61	32	hcv	hcv	PROPN
ajlm-269	61	33	rna	rna	PROPN
ajlm-269	61	34	.	.	PUNCT
ajlm-269	62	1	template	template	NOUN
ajlm-269	62	2	volumes	volume	NOUN
ajlm-269	62	3	of	of	ADP
ajlm-269	62	4	1	1	NUM
ajlm-269	62	5	µl	µl	NOUN
ajlm-269	62	6	,	,	PUNCT
ajlm-269	62	7	5	5	NUM
ajlm-269	62	8	µl	µl	ADP
ajlm-269	62	9	and	and	CCONJ
ajlm-269	62	10	10	10	NUM
ajlm-269	62	11	µl	µl	NOUN
ajlm-269	62	12	were	be	AUX
ajlm-269	62	13	used	use	VERB
ajlm-269	62	14	to	to	PART
ajlm-269	62	15	determine	determine	VERB
ajlm-269	62	16	the	the	DET
ajlm-269	62	17	optimal	optimal	ADJ
ajlm-269	62	18	starting	starting	NOUN
ajlm-269	62	19	concentration	concentration	NOUN
ajlm-269	62	20	of	of	ADP
ajlm-269	62	21	the	the	DET
ajlm-269	62	22	template	template	NOUN
ajlm-269	62	23	rna	rna	NOUN
ajlm-269	62	24	.	.	PUNCT
ajlm-269	63	1	the	the	DET
ajlm-269	63	2	hcv	hcv	PROPN
ajlm-269	63	3	quantitative	quantitative	ADJ
ajlm-269	63	4	pcr	pcr	ADJ
ajlm-269	63	5	proficiency	proficiency	NOUN
ajlm-269	63	6	panel	panel	NOUN
ajlm-269	63	7	(	(	PUNCT
ajlm-269	63	8	qcmd	qcmd	PROPN
ajlm-269	63	9	,	,	PUNCT
ajlm-269	63	10	glasgow	glasgow	PROPN
ajlm-269	63	11	,	,	PUNCT
ajlm-269	63	12	scotland	scotland	PROPN
ajlm-269	63	13	)	)	PUNCT
ajlm-269	63	14	was	be	AUX
ajlm-269	63	15	prepared	prepare	VERB
ajlm-269	63	16	in	in	ADP
ajlm-269	63	17	five	five	NUM
ajlm-269	63	18	-	-	ADJ
ajlm-269	63	19	fold	fold	ADJ
ajlm-269	63	20	dilutions	dilution	NOUN
ajlm-269	63	21	as	as	ADP
ajlm-269	63	22	an	an	DET
ajlm-269	63	23	external	external	ADJ
ajlm-269	63	24	quality	quality	NOUN
ajlm-269	63	25	control	control	NOUN
ajlm-269	63	26	standard	standard	NOUN
ajlm-269	63	27	to	to	PART
ajlm-269	63	28	calculate	calculate	VERB
ajlm-269	63	29	the	the	DET
ajlm-269	63	30	lower	low	ADJ
ajlm-269	63	31	limit	limit	NOUN
ajlm-269	63	32	of	of	ADP
ajlm-269	63	33	detection	detection	NOUN
ajlm-269	63	34	of	of	ADP
ajlm-269	63	35	the	the	DET
ajlm-269	63	36	optimised	optimise	VERB
ajlm-269	63	37	hcv	hcv	PROPN
ajlm-269	63	38	rna	rna	PROPN
ajlm-269	63	39	pcr	pcr	NOUN
ajlm-269	63	40	assay	assay	VERB
ajlm-269	63	41	.	.	PUNCT
ajlm-269	64	1	for	for	ADP
ajlm-269	64	2	the	the	DET
ajlm-269	64	3	preparation	preparation	NOUN
ajlm-269	64	4	of	of	ADP
ajlm-269	64	5	the	the	DET
ajlm-269	64	6	in	in	ADP
ajlm-269	64	7	-	-	PUNCT
ajlm-269	64	8	house	house	NOUN
ajlm-269	64	9	internal	internal	ADJ
ajlm-269	64	10	quality	quality	NOUN
ajlm-269	64	11	control	control	NOUN
ajlm-269	64	12	specimens	specimen	NOUN
ajlm-269	64	13	,	,	PUNCT
ajlm-269	64	14	hcv	hcv	ADJ
ajlm-269	64	15	-	-	PUNCT
ajlm-269	64	16	negative	negative	ADJ
ajlm-269	64	17	whole	whole	ADJ
ajlm-269	64	18	blood	blood	NOUN
ajlm-269	64	19	was	be	AUX
ajlm-269	64	20	provided	provide	VERB
ajlm-269	64	21	by	by	ADP
ajlm-269	64	22	the	the	DET
ajlm-269	64	23	south	south	ADJ
ajlm-269	64	24	african	african	ADJ
ajlm-269	64	25	national	national	ADJ
ajlm-269	64	26	blood	blood	NOUN
ajlm-269	64	27	services	service	NOUN
ajlm-269	64	28	(	(	PUNCT
ajlm-269	64	29	durban	durban	PROPN
ajlm-269	64	30	,	,	PUNCT
ajlm-269	64	31	south	south	PROPN
ajlm-269	64	32	africa	africa	PROPN
ajlm-269	64	33	)	)	PUNCT
ajlm-269	64	34	.	.	PUNCT
ajlm-269	65	1	these	these	DET
ajlm-269	65	2	dbs	dbs	PROPN
ajlm-269	65	3	specimens	specimen	NOUN
ajlm-269	65	4	were	be	AUX
ajlm-269	65	5	prepared	prepare	VERB
ajlm-269	65	6	by	by	ADP
ajlm-269	65	7	spiking	spike	VERB
ajlm-269	65	8	the	the	DET
ajlm-269	65	9	hcv	hcv	ADJ
ajlm-269	65	10	-	-	PUNCT
ajlm-269	65	11	negative	negative	ADJ
ajlm-269	65	12	whole	whole	ADJ
ajlm-269	65	13	blood	blood	NOUN
ajlm-269	65	14	with	with	ADP
ajlm-269	65	15	hcv	hcv	ADJ
ajlm-269	65	16	-	-	PUNCT
ajlm-269	65	17	positive	positive	ADJ
ajlm-269	65	18	plasma	plasma	NOUN
ajlm-269	65	19	(	(	PUNCT
ajlm-269	65	20	3	3	NUM
ajlm-269	65	21	580	580	NUM
ajlm-269	65	22	000	000	NUM
ajlm-269	65	23	iu	iu	NOUN
ajlm-269	65	24	/	/	SYM
ajlm-269	65	25	ml	ml	VERB
ajlm-269	65	26	)	)	PUNCT
ajlm-269	65	27	obtained	obtain	VERB
ajlm-269	65	28	from	from	ADP
ajlm-269	65	29	the	the	DET
ajlm-269	65	30	national	national	PROPN
ajlm-269	65	31	institute	institute	PROPN
ajlm-269	65	32	of	of	ADP
ajlm-269	65	33	communicable	communicable	NOUN
ajlm-269	65	34	diseases	disease	NOUN
ajlm-269	65	35	(	(	PUNCT
ajlm-269	65	36	sandringham	sandringham	PROPN
ajlm-269	65	37	,	,	PUNCT
ajlm-269	65	38	johannesburg	johannesburg	PROPN
ajlm-269	65	39	,	,	PUNCT
ajlm-269	65	40	south	south	PROPN
ajlm-269	65	41	africa	africa	PROPN
ajlm-269	65	42	)	)	PUNCT
ajlm-269	65	43	.	.	PUNCT
ajlm-269	66	1	this	this	DET
ajlm-269	66	2	plasma	plasma	NOUN
ajlm-269	66	3	was	be	AUX
ajlm-269	66	4	tested	test	VERB
ajlm-269	66	5	for	for	ADP
ajlm-269	66	6	hcv	hcv	NOUN
ajlm-269	66	7	using	use	VERB
ajlm-269	66	8	the	the	DET
ajlm-269	66	9	cobas	cobas	PROPN
ajlm-269	66	10	ampliprep	ampliprep	PROPN
ajlm-269	66	11	/	/	SYM
ajlm-269	66	12	cobas	cobas	ADJ
ajlm-269	66	13	taqman	taqman	NOUN
ajlm-269	66	14	hepatitis	hepatitis	PROPN
ajlm-269	66	15	c	c	PROPN
ajlm-269	66	16	virus	virus	NOUN
ajlm-269	66	17	assay	assay	VERB
ajlm-269	66	18	on	on	ADP
ajlm-269	66	19	the	the	DET
ajlm-269	66	20	cobas	cobas	PROPN
ajlm-269	66	21	ampliprep	ampliprep	PROPN
ajlm-269	66	22	and	and	CCONJ
ajlm-269	66	23	cobas	cobas	ADJ
ajlm-269	66	24	taqman	taqman	NOUN
ajlm-269	66	25	48	48	NUM
ajlm-269	66	26	analyser	analyser	NOUN
ajlm-269	66	27	(	(	PUNCT
ajlm-269	66	28	roche	roche	NOUN
ajlm-269	66	29	diagnostics	diagnostic	NOUN
ajlm-269	66	30	,	,	PUNCT
ajlm-269	66	31	mannheim	mannheim	PROPN
ajlm-269	66	32	,	,	PUNCT
ajlm-269	66	33	germany	germany	PROPN
ajlm-269	66	34	)	)	PUNCT
ajlm-269	66	35	.	.	PUNCT
ajlm-269	67	1	ten	ten	NUM
ajlm-269	67	2	-	-	ADJ
ajlm-269	67	3	fold	fold	ADJ
ajlm-269	67	4	serial	serial	ADJ
ajlm-269	67	5	dilutions	dilution	NOUN
ajlm-269	67	6	were	be	AUX
ajlm-269	67	7	made	make	VERB
ajlm-269	67	8	,	,	PUNCT
ajlm-269	67	9	with	with	ADP
ajlm-269	67	10	a	a	DET
ajlm-269	67	11	starting	starting	NOUN
ajlm-269	67	12	concentration	concentration	NOUN
ajlm-269	67	13	of	of	ADP
ajlm-269	67	14	3	3	NUM
ajlm-269	67	15	580	580	NUM
ajlm-269	67	16	000	000	NUM
ajlm-269	67	17	iu	iu	NOUN
ajlm-269	67	18	/	/	SYM
ajlm-269	67	19	ml	ml	X
ajlm-269	67	20	of	of	ADP
ajlm-269	67	21	hcv	hcv	PROPN
ajlm-269	67	22	rna	rna	PROPN
ajlm-269	67	23	,	,	PUNCT
ajlm-269	67	24	resulting	result	VERB
ajlm-269	67	25	in	in	ADP
ajlm-269	67	26	values	value	NOUN
ajlm-269	67	27	of	of	ADP
ajlm-269	67	28	3	3	NUM
ajlm-269	67	29	580	580	NUM
ajlm-269	67	30	000	000	NUM
ajlm-269	67	31	iu	iu	NOUN
ajlm-269	67	32	/	/	SYM
ajlm-269	67	33	ml	ml	NOUN
ajlm-269	67	34	,	,	PUNCT
ajlm-269	67	35	358	358	NUM
ajlm-269	67	36	000	000	NUM
ajlm-269	67	37	iu	iu	ADP
ajlm-269	67	38	/	/	SYM
ajlm-269	67	39	ml	ml	NOUN
ajlm-269	67	40	,	,	PUNCT
ajlm-269	67	41	35	35	NUM
ajlm-269	67	42	800	800	NUM
ajlm-269	67	43	iu	iu	ADP
ajlm-269	67	44	/	/	SYM
ajlm-269	67	45	ml	ml	NOUN
ajlm-269	67	46	and	and	CCONJ
ajlm-269	67	47	3580	3580	NUM
ajlm-269	67	48	iu	iu	NOUN
ajlm-269	67	49	/	/	SYM
ajlm-269	67	50	ml	ml	NOUN
ajlm-269	67	51	.	.	PUNCT
ajlm-269	67	52	unspiked	unspike	VERB
ajlm-269	67	53	whole	whole	ADJ
ajlm-269	67	54	blood	blood	NOUN
ajlm-269	67	55	was	be	AUX
ajlm-269	67	56	used	use	VERB
ajlm-269	67	57	as	as	ADP
ajlm-269	67	58	a	a	DET
ajlm-269	67	59	negative	negative	ADJ
ajlm-269	67	60	control	control	NOUN
ajlm-269	67	61	.	.	PUNCT
ajlm-269	68	1	fifty	fifty	NUM
ajlm-269	68	2	microliters	microliter	NOUN
ajlm-269	68	3	(	(	PUNCT
ajlm-269	68	4	50	50	NUM
ajlm-269	68	5	µl	µl	NOUN
ajlm-269	68	6	)	)	PUNCT
ajlm-269	68	7	of	of	ADP
ajlm-269	68	8	the	the	DET
ajlm-269	68	9	positive	positive	ADJ
ajlm-269	68	10	and	and	CCONJ
ajlm-269	68	11	negative	negative	ADJ
ajlm-269	68	12	internal	internal	ADJ
ajlm-269	68	13	control	control	NOUN
ajlm-269	68	14	specimens	specimen	NOUN
ajlm-269	68	15	were	be	AUX
ajlm-269	68	16	spotted	spot	VERB
ajlm-269	68	17	onto	onto	ADP
ajlm-269	68	18	whatman	whatman	NOUN
ajlm-269	68	19	filter	filter	NOUN
ajlm-269	68	20	paper	paper	NOUN
ajlm-269	68	21	to	to	PART
ajlm-269	68	22	mimic	mimic	VERB
ajlm-269	68	23	infant	infant	NOUN
ajlm-269	68	24	dbs	dbs	PROPN
ajlm-269	68	25	specimens	specimen	NOUN
ajlm-269	68	26	.	.	PROPN
ajlm-269	69	1	results	result	VERB
ajlm-269	69	2	primer	primer	NOUN
ajlm-269	69	3	concentrations	concentration	NOUN
ajlm-269	69	4	adjusted	adjust	VERB
ajlm-269	69	5	to	to	ADP
ajlm-269	69	6	0.25	0.25	NUM
ajlm-269	69	7	µm	µm	NOUN
ajlm-269	69	8	and	and	CCONJ
ajlm-269	69	9	a	a	DET
ajlm-269	69	10	template	template	NOUN
ajlm-269	69	11	volume	volume	NOUN
ajlm-269	69	12	of	of	ADP
ajlm-269	69	13	10	10	NUM
ajlm-269	69	14	µl	µl	ADP
ajlm-269	69	15	improved	improve	VERB
ajlm-269	69	16	pcr	pcr	ADJ
ajlm-269	69	17	amplification	amplification	NOUN
ajlm-269	69	18	.	.	PUNCT
ajlm-269	70	1	an	an	DET
ajlm-269	70	2	annealing	anneal	VERB
ajlm-269	70	3	temperature	temperature	NOUN
ajlm-269	70	4	of	of	ADP
ajlm-269	70	5	58	58	NUM
ajlm-269	70	6	°	°	NOUN
ajlm-269	70	7	c	c	NOUN
ajlm-269	70	8	resulted	result	VERB
ajlm-269	70	9	in	in	ADP
ajlm-269	70	10	higher	high	ADJ
ajlm-269	70	11	levels	level	NOUN
ajlm-269	70	12	of	of	ADP
ajlm-269	70	13	amplified	amplify	VERB
ajlm-269	70	14	pcr	pcr	ADJ
ajlm-269	70	15	product	product	NOUN
ajlm-269	70	16	compared	compare	VERB
ajlm-269	70	17	with	with	ADP
ajlm-269	70	18	an	an	DET
ajlm-269	70	19	annealing	anneal	VERB
ajlm-269	70	20	temperature	temperature	NOUN
ajlm-269	70	21	of	of	ADP
ajlm-269	70	22	65	65	NUM
ajlm-269	70	23	°	°	NOUN
ajlm-269	70	24	c	c	NOUN
ajlm-269	70	25	.	.	PUNCT
ajlm-269	71	1	quality	quality	NOUN
ajlm-269	71	2	control	control	NOUN
ajlm-269	71	3	specimens	specimen	NOUN
ajlm-269	71	4	with	with	ADP
ajlm-269	71	5	hcv	hcv	PROPN
ajlm-269	71	6	rna	rna	NOUN
ajlm-269	71	7	values	value	NOUN
ajlm-269	71	8	of	of	ADP
ajlm-269	71	9	<	<	X
ajlm-269	71	10	10	10	NUM
ajlm-269	71	11	000	000	NUM
ajlm-269	71	12	iu	iu	ADP
ajlm-269	71	13	/	/	SYM
ajlm-269	71	14	ml	ml	NOUN
ajlm-269	71	15	were	be	AUX
ajlm-269	71	16	detectable	detectable	ADJ
ajlm-269	71	17	at	at	ADP
ajlm-269	71	18	an	an	DET
ajlm-269	71	19	annealing	anneal	VERB
ajlm-269	71	20	temperature	temperature	NOUN
ajlm-269	71	21	of	of	ADP
ajlm-269	71	22	58	58	NUM
ajlm-269	71	23	°	°	NOUN
ajlm-269	71	24	c	c	NOUN
ajlm-269	71	25	.	.	PUNCT
ajlm-269	72	1	detection	detection	NOUN
ajlm-269	72	2	at	at	ADP
ajlm-269	72	3	a	a	DET
ajlm-269	72	4	melting	melt	VERB
ajlm-269	72	5	temperature	temperature	NOUN
ajlm-269	72	6	of	of	ADP
ajlm-269	72	7	86	86	NUM
ajlm-269	72	8	°	°	NOUN
ajlm-269	72	9	c	c	NOUN
ajlm-269	72	10	was	be	AUX
ajlm-269	72	11	indicative	indicative	ADJ
ajlm-269	72	12	of	of	ADP
ajlm-269	72	13	an	an	DET
ajlm-269	72	14	hcv	hcv	ADJ
ajlm-269	72	15	-	-	PUNCT
ajlm-269	72	16	positive	positive	ADJ
ajlm-269	72	17	result	result	NOUN
ajlm-269	72	18	(	(	PUNCT
ajlm-269	72	19	figure	figure	NOUN
ajlm-269	72	20	1	1	NUM
ajlm-269	72	21	)	)	PUNCT
ajlm-269	72	22	for	for	ADP
ajlm-269	72	23	quality	quality	NOUN
ajlm-269	72	24	control	control	NOUN
ajlm-269	72	25	specimens	specimen	NOUN
ajlm-269	72	26	with	with	ADP
ajlm-269	72	27	viral	viral	ADJ
ajlm-269	72	28	loads	load	NOUN
ajlm-269	72	29	of	of	ADP
ajlm-269	72	30	13	13	NUM
ajlm-269	72	31	646	646	NUM
ajlm-269	72	32	iu	iu	NOUN
ajlm-269	72	33	/	/	SYM
ajlm-269	72	34	ml	ml	PROPN
ajlm-269	72	35	(	(	PUNCT
ajlm-269	72	36	high	high	ADJ
ajlm-269	72	37	)	)	PUNCT
ajlm-269	72	38	,	,	PUNCT
ajlm-269	72	39	7063	7063	NUM
ajlm-269	72	40	iu	iu	ADP
ajlm-269	72	41	/	/	SYM
ajlm-269	72	42	ml	ml	PROPN
ajlm-269	72	43	(	(	PUNCT
ajlm-269	72	44	medium	medium	NOUN
ajlm-269	72	45	)	)	PUNCT
ajlm-269	72	46	and	and	CCONJ
ajlm-269	72	47	3972	3972	NUM
ajlm-269	72	48	iu	iu	NOUN
ajlm-269	72	49	/	/	SYM
ajlm-269	72	50	ml	ml	PROPN
ajlm-269	72	51	(	(	PUNCT
ajlm-269	72	52	low	low	ADJ
ajlm-269	72	53	)	)	PUNCT
ajlm-269	72	54	.	.	PUNCT
ajlm-269	73	1	the	the	DET
ajlm-269	73	2	external	external	ADJ
ajlm-269	73	3	quality	quality	NOUN
ajlm-269	73	4	control	control	NOUN
ajlm-269	73	5	specimens	specimen	NOUN
ajlm-269	73	6	with	with	ADP
ajlm-269	73	7	known	know	VERB
ajlm-269	73	8	hcv	hcv	VERB
ajlm-269	73	9	viral	viral	ADJ
ajlm-269	73	10	loads	load	NOUN
ajlm-269	73	11	of	of	ADP
ajlm-269	73	12	30	30	NUM
ajlm-269	73	13	000	000	NUM
ajlm-269	73	14	iu	iu	NOUN
ajlm-269	73	15	/	/	SYM
ajlm-269	73	16	ml	ml	NOUN
ajlm-269	73	17	,	,	PUNCT
ajlm-269	73	18	6000	6000	NUM
ajlm-269	73	19	iu	iu	ADP
ajlm-269	73	20	/	/	SYM
ajlm-269	73	21	ml	ml	NOUN
ajlm-269	73	22	and	and	CCONJ
ajlm-269	73	23	1200	1200	NUM
ajlm-269	73	24	iu	iu	ADP
ajlm-269	73	25	/	/	SYM
ajlm-269	73	26	ml	ml	NOUN
ajlm-269	73	27	were	be	AUX
ajlm-269	73	28	detected	detect	VERB
ajlm-269	73	29	by	by	ADP
ajlm-269	73	30	the	the	DET
ajlm-269	73	31	pcr	pcr	NOUN
ajlm-269	73	32	,	,	PUNCT
ajlm-269	73	33	whereas	whereas	SCONJ
ajlm-269	73	34	external	external	ADJ
ajlm-269	73	35	quality	quality	NOUN
ajlm-269	73	36	control	control	NOUN
ajlm-269	73	37	specimens	specimen	NOUN
ajlm-269	73	38	with	with	ADP
ajlm-269	73	39	an	an	DET
ajlm-269	73	40	hcv	hcv	ADJ
ajlm-269	73	41	viral	viral	ADJ
ajlm-269	73	42	load	load	NOUN
ajlm-269	73	43	of	of	ADP
ajlm-269	73	44	240	240	NUM
ajlm-269	73	45	iu	iu	NOUN
ajlm-269	73	46	/	/	SYM
ajlm-269	73	47	ml	ml	NOUN
ajlm-269	73	48	were	be	AUX
ajlm-269	73	49	undetectable	undetectable	ADJ
ajlm-269	73	50	(	(	PUNCT
ajlm-269	73	51	figure	figure	NOUN
ajlm-269	73	52	2	2	NUM
ajlm-269	73	53	)	)	PUNCT
ajlm-269	73	54	.	.	PUNCT
ajlm-269	74	1	the	the	DET
ajlm-269	74	2	in	in	ADP
ajlm-269	74	3	-	-	PUNCT
ajlm-269	74	4	house	house	NOUN
ajlm-269	74	5	hcv	hcv	PROPN
ajlm-269	74	6	quality	quality	NOUN
ajlm-269	74	7	control	control	NOUN
ajlm-269	74	8	specimens	specimen	NOUN
ajlm-269	74	9	with	with	ADP
ajlm-269	74	10	viral	viral	ADJ
ajlm-269	74	11	loads	load	NOUN
ajlm-269	74	12	of	of	ADP
ajlm-269	74	13	3	3	NUM
ajlm-269	74	14	580	580	NUM
ajlm-269	74	15	000	000	NUM
ajlm-269	74	16	iu	iu	NOUN
ajlm-269	74	17	/	/	SYM
ajlm-269	74	18	ml	ml	NOUN
ajlm-269	74	19	,	,	PUNCT
ajlm-269	74	20	358	358	NUM
ajlm-269	74	21	000	000	NUM
ajlm-269	74	22	iu	iu	ADP
ajlm-269	74	23	/	/	SYM
ajlm-269	74	24	ml	ml	NOUN
ajlm-269	74	25	,	,	PUNCT
ajlm-269	74	26	35	35	NUM
ajlm-269	74	27	800	800	NUM
ajlm-269	74	28	iu	iu	ADP
ajlm-269	74	29	/	/	SYM
ajlm-269	74	30	ml	ml	NOUN
ajlm-269	74	31	and	and	CCONJ
ajlm-269	74	32	3580	3580	NUM
ajlm-269	74	33	iu	iu	NOUN
ajlm-269	74	34	/	/	SYM
ajlm-269	74	35	ml	ml	NOUN
ajlm-269	74	36	were	be	AUX
ajlm-269	74	37	all	all	ADV
ajlm-269	74	38	detected	detect	VERB
ajlm-269	74	39	by	by	ADP
ajlm-269	74	40	the	the	DET
ajlm-269	74	41	hcv	hcv	PROPN
ajlm-269	74	42	pcr	pcr	NOUN
ajlm-269	74	43	(	(	PUNCT
ajlm-269	74	44	figure	figure	NOUN
ajlm-269	74	45	3	3	NUM
ajlm-269	74	46	)	)	PUNCT
ajlm-269	74	47	.	.	PUNCT
ajlm-269	75	1	figure	figure	VERB
ajlm-269	75	2	1	1	NUM
ajlm-269	75	3	:	:	PUNCT
ajlm-269	75	4	melting	melt	VERB
ajlm-269	75	5	curves	curve	NOUN
ajlm-269	75	6	at	at	ADP
ajlm-269	75	7	a	a	DET
ajlm-269	75	8	melting	melt	VERB
ajlm-269	75	9	temperature	temperature	NOUN
ajlm-269	75	10	of	of	ADP
ajlm-269	75	11	86	86	NUM
ajlm-269	75	12	°	°	NOUN
ajlm-269	75	13	c	c	NOUN
ajlm-269	75	14	represent	represent	VERB
ajlm-269	75	15	hcv	hcv	ADJ
ajlm-269	75	16	-	-	PUNCT
ajlm-269	75	17	positive	positive	ADJ
ajlm-269	75	18	controls	control	NOUN
ajlm-269	75	19	.	.	PUNCT
ajlm-269	76	1	sixteen	sixteen	NUM
ajlm-269	76	2	specimens	specimen	NOUN
ajlm-269	76	3	that	that	PRON
ajlm-269	76	4	tested	test	VERB
ajlm-269	76	5	negative	negative	ADJ
ajlm-269	76	6	for	for	SCONJ
ajlm-269	76	7	hcv	hcv	NOUN
ajlm-269	76	8	by	by	ADP
ajlm-269	76	9	serology	serology	NOUN
ajlm-269	76	10	were	be	AUX
ajlm-269	76	11	used	use	VERB
ajlm-269	76	12	as	as	ADP
ajlm-269	76	13	hcv	hcv	NOUN
ajlm-269	76	14	-	-	PUNCT
ajlm-269	76	15	negative	negative	ADJ
ajlm-269	76	16	controls	control	NOUN
ajlm-269	76	17	.	.	PUNCT
ajlm-269	77	1	figure	figure	NOUN
ajlm-269	77	2	2	2	NUM
ajlm-269	77	3	:	:	PUNCT
ajlm-269	77	4	external	external	ADJ
ajlm-269	77	5	quality	quality	NOUN
ajlm-269	77	6	controls	control	NOUN
ajlm-269	77	7	.	.	PUNCT
ajlm-269	78	1	the	the	DET
ajlm-269	78	2	melting	melting	NOUN
ajlm-269	78	3	curves	curve	NOUN
ajlm-269	78	4	show	show	VERB
ajlm-269	78	5	results	result	NOUN
ajlm-269	78	6	of	of	ADP
ajlm-269	78	7	five	five	NUM
ajlm-269	78	8	-	-	ADJ
ajlm-269	78	9	fold	fold	ADJ
ajlm-269	78	10	dilutions	dilution	NOUN
ajlm-269	78	11	of	of	ADP
ajlm-269	78	12	hcv	hcv	ADJ
ajlm-269	78	13	-	-	PUNCT
ajlm-269	78	14	positive	positive	ADJ
ajlm-269	78	15	external	external	ADJ
ajlm-269	78	16	quality	quality	NOUN
ajlm-269	78	17	control	control	NOUN
ajlm-269	78	18	specimens	specimen	NOUN
ajlm-269	78	19	.	.	PUNCT
ajlm-269	79	1	these	these	DET
ajlm-269	79	2	specimens	specimen	NOUN
ajlm-269	79	3	were	be	AUX
ajlm-269	79	4	used	use	VERB
ajlm-269	79	5	to	to	PART
ajlm-269	79	6	determine	determine	VERB
ajlm-269	79	7	the	the	DET
ajlm-269	79	8	lowest	low	ADJ
ajlm-269	79	9	hcv	hcv	X
ajlm-269	79	10	rna	rna	NOUN
ajlm-269	79	11	-	-	PUNCT
ajlm-269	79	12	positive	positive	ADJ
ajlm-269	79	13	concentrations	concentration	NOUN
ajlm-269	79	14	that	that	PRON
ajlm-269	79	15	the	the	DET
ajlm-269	79	16	optimised	optimise	VERB
ajlm-269	79	17	pcr	pcr	NOUN
ajlm-269	79	18	assay	assay	NOUN
ajlm-269	79	19	could	could	AUX
ajlm-269	79	20	detect	detect	VERB
ajlm-269	79	21	.	.	PUNCT
ajlm-269	80	1	hcv	hcv	PROPN
ajlm-269	80	2	rna	rna	PROPN
ajlm-269	80	3	at	at	ADP
ajlm-269	80	4	concentrations	concentration	NOUN
ajlm-269	80	5	of	of	ADP
ajlm-269	80	6	30	30	NUM
ajlm-269	80	7	000	000	NUM
ajlm-269	80	8	iu	iu	NOUN
ajlm-269	80	9	/	/	SYM
ajlm-269	80	10	ml	ml	NOUN
ajlm-269	80	11	,	,	PUNCT
ajlm-269	80	12	6000	6000	NUM
ajlm-269	80	13	iu	iu	ADP
ajlm-269	80	14	/	/	SYM
ajlm-269	80	15	ml	ml	NOUN
ajlm-269	80	16	and	and	CCONJ
ajlm-269	80	17	1200	1200	NUM
ajlm-269	80	18	iu	iu	ADP
ajlm-269	80	19	/	/	SYM
ajlm-269	80	20	ml	ml	NOUN
ajlm-269	80	21	were	be	AUX
ajlm-269	80	22	detected	detect	VERB
ajlm-269	80	23	by	by	ADP
ajlm-269	80	24	the	the	DET
ajlm-269	80	25	assay	assay	NOUN
ajlm-269	80	26	,	,	PUNCT
ajlm-269	80	27	whereas	whereas	SCONJ
ajlm-269	80	28	concentrations	concentration	NOUN
ajlm-269	80	29	of	of	ADP
ajlm-269	80	30	240	240	NUM
ajlm-269	80	31	iu	iu	NOUN
ajlm-269	80	32	/	/	SYM
ajlm-269	80	33	ml	ml	X
ajlm-269	80	34	of	of	ADP
ajlm-269	80	35	hcv	hcv	PROPN
ajlm-269	80	36	rna	rna	NOUN
ajlm-269	80	37	and	and	CCONJ
ajlm-269	80	38	lower	low	ADJ
ajlm-269	80	39	were	be	AUX
ajlm-269	80	40	undetectable	undetectable	ADJ
ajlm-269	80	41	.	.	PUNCT
ajlm-269	81	1	figure	figure	VERB
ajlm-269	81	2	3	3	NUM
ajlm-269	81	3	:	:	PUNCT
ajlm-269	81	4	internal	internal	ADJ
ajlm-269	81	5	quality	quality	NOUN
ajlm-269	81	6	controls	control	NOUN
ajlm-269	81	7	.	.	PUNCT
ajlm-269	82	1	internal	internal	ADJ
ajlm-269	82	2	quality	quality	NOUN
ajlm-269	82	3	control	control	NOUN
ajlm-269	82	4	dbs	dbs	PROPN
ajlm-269	82	5	specimens	specimen	NOUN
ajlm-269	82	6	were	be	AUX
ajlm-269	82	7	prepared	prepare	VERB
ajlm-269	82	8	by	by	ADP
ajlm-269	82	9	spiking	spike	VERB
ajlm-269	82	10	hcv	hcv	ADJ
ajlm-269	82	11	-	-	PUNCT
ajlm-269	82	12	negative	negative	ADJ
ajlm-269	82	13	whole	whole	ADJ
ajlm-269	82	14	blood	blood	NOUN
ajlm-269	82	15	with	with	ADP
ajlm-269	82	16	hcv	hcv	ADJ
ajlm-269	82	17	-	-	PUNCT
ajlm-269	82	18	positive	positive	ADJ
ajlm-269	82	19	plasma	plasma	NOUN
ajlm-269	82	20	.	.	PUNCT
ajlm-269	83	1	ten	ten	NUM
ajlm-269	83	2	-	-	ADJ
ajlm-269	83	3	fold	fold	ADJ
ajlm-269	83	4	dilutions	dilution	NOUN
ajlm-269	83	5	were	be	AUX
ajlm-269	83	6	prepared	prepare	VERB
ajlm-269	83	7	with	with	ADP
ajlm-269	83	8	a	a	DET
ajlm-269	83	9	starting	starting	NOUN
ajlm-269	83	10	concentration	concentration	NOUN
ajlm-269	83	11	of	of	ADP
ajlm-269	83	12	3580	3580	NUM
ajlm-269	83	13	000	000	NUM
ajlm-269	83	14	iu	iu	NOUN
ajlm-269	83	15	/	/	SYM
ajlm-269	83	16	ml	ml	X
ajlm-269	83	17	of	of	ADP
ajlm-269	83	18	hcv	hcv	PROPN
ajlm-269	83	19	rna	rna	PROPN
ajlm-269	83	20	and	and	CCONJ
ajlm-269	83	21	added	add	VERB
ajlm-269	83	22	onto	onto	ADP
ajlm-269	83	23	whatman	whatman	NOUN
ajlm-269	83	24	filter	filter	NOUN
ajlm-269	83	25	paper	paper	NOUN
ajlm-269	83	26	.	.	PUNCT
ajlm-269	84	1	hcv	hcv	ADJ
ajlm-269	84	2	-	-	PUNCT
ajlm-269	84	3	negative	negative	ADJ
ajlm-269	84	4	whole	whole	ADJ
ajlm-269	84	5	blood	blood	NOUN
ajlm-269	84	6	was	be	AUX
ajlm-269	84	7	used	use	VERB
ajlm-269	84	8	as	as	ADP
ajlm-269	84	9	a	a	DET
ajlm-269	84	10	negative	negative	ADJ
ajlm-269	84	11	control	control	NOUN
ajlm-269	84	12	.	.	PUNCT
ajlm-269	85	1	an	an	DET
ajlm-269	85	2	hcv	hcv	PROPN
ajlm-269	85	3	rna	rna	NOUN
ajlm-269	85	4	concentration	concentration	NOUN
ajlm-269	85	5	of	of	ADP
ajlm-269	85	6	3580	3580	NUM
ajlm-269	85	7	iu	iu	NOUN
ajlm-269	85	8	/	/	SYM
ajlm-269	85	9	ml	ml	PROPN
ajlm-269	85	10	was	be	AUX
ajlm-269	85	11	the	the	DET
ajlm-269	85	12	lowest	low	ADJ
ajlm-269	85	13	concentration	concentration	NOUN
ajlm-269	85	14	of	of	ADP
ajlm-269	85	15	rna	rna	NOUN
ajlm-269	85	16	detected	detect	VERB
ajlm-269	85	17	for	for	ADP
ajlm-269	85	18	dbs	dbs	NOUN
ajlm-269	85	19	using	use	VERB
ajlm-269	85	20	the	the	DET
ajlm-269	85	21	optimised	optimise	VERB
ajlm-269	85	22	pcr	pcr	NOUN
ajlm-269	85	23	assay	assay	VERB
ajlm-269	85	24	.	.	PUNCT
ajlm-269	86	1	the	the	DET
ajlm-269	86	2	lowest	low	ADJ
ajlm-269	86	3	concentration	concentration	NOUN
ajlm-269	86	4	detected	detect	VERB
ajlm-269	86	5	by	by	ADP
ajlm-269	86	6	the	the	DET
ajlm-269	86	7	optimised	optimise	VERB
ajlm-269	86	8	hcv	hcv	PROPN
ajlm-269	86	9	pcr	pcr	PROPN
ajlm-269	86	10	was	be	AUX
ajlm-269	86	11	1200	1200	NUM
ajlm-269	86	12	iu	iu	ADP
ajlm-269	86	13	/	/	SYM
ajlm-269	86	14	ml	ml	PROPN
ajlm-269	86	15	(	(	PUNCT
ajlm-269	86	16	figure	figure	NOUN
ajlm-269	86	17	2	2	NUM
ajlm-269	86	18	)	)	PUNCT
ajlm-269	86	19	when	when	SCONJ
ajlm-269	86	20	using	use	VERB
ajlm-269	86	21	the	the	DET
ajlm-269	86	22	qcmd	qcmd	PROPN
ajlm-269	86	23	quality	quality	NOUN
ajlm-269	86	24	control	control	NOUN
ajlm-269	86	25	specimens	specimen	NOUN
ajlm-269	86	26	.	.	PUNCT
ajlm-269	87	1	the	the	DET
ajlm-269	87	2	in	in	ADP
ajlm-269	87	3	-	-	PUNCT
ajlm-269	87	4	house	house	NOUN
ajlm-269	87	5	internal	internal	ADJ
ajlm-269	87	6	quality	quality	NOUN
ajlm-269	87	7	control	control	PROPN
ajlm-269	87	8	dbs	dbs	PROPN
ajlm-269	87	9	specimens	specimen	NOUN
ajlm-269	87	10	were	be	AUX
ajlm-269	87	11	detected	detect	VERB
ajlm-269	87	12	with	with	ADP
ajlm-269	87	13	viral	viral	ADJ
ajlm-269	87	14	loads	load	NOUN
ajlm-269	87	15	as	as	ADV
ajlm-269	87	16	low	low	ADJ
ajlm-269	87	17	as	as	ADP
ajlm-269	87	18	3580	3580	NUM
ajlm-269	87	19	iu	iu	NOUN
ajlm-269	87	20	/	/	SYM
ajlm-269	87	21	ml	ml	X
ajlm-269	87	22	of	of	ADP
ajlm-269	87	23	hcv	hcv	PROPN
ajlm-269	87	24	rna	rna	PROPN
ajlm-269	87	25	(	(	PUNCT
ajlm-269	87	26	figure	figure	NOUN
ajlm-269	87	27	3	3	NUM
ajlm-269	87	28	)	)	PUNCT
ajlm-269	87	29	.	.	PUNCT
ajlm-269	88	1	of	of	ADP
ajlm-269	88	2	the	the	DET
ajlm-269	88	3	179	179	NUM
ajlm-269	88	4	infant	infant	NOUN
ajlm-269	88	5	dbs	dbs	PROPN
ajlm-269	88	6	specimens	specimen	NOUN
ajlm-269	88	7	,	,	PUNCT
ajlm-269	88	8	13	13	NUM
ajlm-269	88	9	tested	test	VERB
ajlm-269	88	10	hiv	hiv	PROPN
ajlm-269	88	11	-	-	PUNCT
ajlm-269	88	12	positive	positive	ADJ
ajlm-269	88	13	and	and	CCONJ
ajlm-269	88	14	166	166	NUM
ajlm-269	88	15	tested	test	VERB
ajlm-269	88	16	hiv	hiv	PROPN
ajlm-269	88	17	-	-	PUNCT
ajlm-269	88	18	negative	negative	ADJ
ajlm-269	88	19	via	via	ADP
ajlm-269	88	20	pcr	pcr	NOUN
ajlm-269	88	21	.	.	PUNCT
ajlm-269	89	1	all	all	DET
ajlm-269	89	2	179	179	NUM
ajlm-269	89	3	dbs	dbs	PROPN
ajlm-269	89	4	specimens	specimen	NOUN
ajlm-269	89	5	tested	test	VERB
ajlm-269	89	6	negative	negative	ADJ
ajlm-269	89	7	for	for	ADP
ajlm-269	89	8	hcv	hcv	NOUN
ajlm-269	89	9	using	use	VERB
ajlm-269	89	10	the	the	DET
ajlm-269	89	11	optimised	optimise	VERB
ajlm-269	89	12	real	real	ADJ
ajlm-269	89	13	-	-	PUNCT
ajlm-269	89	14	time	time	NOUN
ajlm-269	89	15	pcr	pcr	NOUN
ajlm-269	89	16	assay	assay	NOUN
ajlm-269	89	17	.	.	PUNCT
ajlm-269	90	1	discussion	discussion	NOUN
ajlm-269	90	2	we	we	PRON
ajlm-269	90	3	optimised	optimise	VERB
ajlm-269	90	4	a	a	DET
ajlm-269	90	5	real	real	ADJ
ajlm-269	90	6	-	-	PUNCT
ajlm-269	90	7	time	time	NOUN
ajlm-269	90	8	pcr	pcr	PROPN
ajlm-269	90	9	test	test	NOUN
ajlm-269	90	10	for	for	ADP
ajlm-269	90	11	hcv	hcv	NOUN
ajlm-269	90	12	using	use	VERB
ajlm-269	90	13	dbs	dbs	NOUN
ajlm-269	90	14	specimens	specimen	NOUN
ajlm-269	90	15	from	from	ADP
ajlm-269	90	16	infants	infant	NOUN
ajlm-269	90	17	as	as	ADP
ajlm-269	90	18	a	a	DET
ajlm-269	90	19	simple	simple	ADJ
ajlm-269	90	20	method	method	NOUN
ajlm-269	90	21	for	for	ADP
ajlm-269	90	22	qualitative	qualitative	ADJ
ajlm-269	90	23	hcv	hcv	NOUN
ajlm-269	90	24	detection	detection	NOUN
ajlm-269	90	25	.	.	PUNCT
ajlm-269	91	1	although	although	SCONJ
ajlm-269	91	2	our	our	PRON
ajlm-269	91	3	method	method	NOUN
ajlm-269	91	4	was	be	AUX
ajlm-269	91	5	qualitative	qualitative	ADJ
ajlm-269	91	6	,	,	PUNCT
ajlm-269	91	7	it	it	PRON
ajlm-269	91	8	was	be	AUX
ajlm-269	91	9	able	able	ADJ
ajlm-269	91	10	to	to	PART
ajlm-269	91	11	detect	detect	VERB
ajlm-269	91	12	at	at	ADV
ajlm-269	91	13	least	least	ADJ
ajlm-269	91	14	3580	3580	NUM
ajlm-269	91	15	iu	iu	NOUN
ajlm-269	91	16	/	/	SYM
ajlm-269	91	17	ml	ml	NOUN
ajlm-269	91	18	and	and	CCONJ
ajlm-269	91	19	1200	1200	NUM
ajlm-269	91	20	iu	iu	AUX
ajlm-269	91	21	/	/	SYM
ajlm-269	91	22	ml	ml	NOUN
ajlm-269	91	23	in	in	ADP
ajlm-269	91	24	various	various	ADJ
ajlm-269	91	25	types	type	NOUN
ajlm-269	91	26	of	of	ADP
ajlm-269	91	27	quality	quality	NOUN
ajlm-269	91	28	control	control	NOUN
ajlm-269	91	29	specimens	specimen	NOUN
ajlm-269	91	30	.	.	PUNCT
ajlm-269	92	1	this	this	PRON
ajlm-269	92	2	could	could	AUX
ajlm-269	92	3	be	be	AUX
ajlm-269	92	4	useful	useful	ADJ
ajlm-269	92	5	for	for	ADP
ajlm-269	92	6	detecting	detect	VERB
ajlm-269	92	7	acute	acute	ADJ
ajlm-269	92	8	hcv	hcv	NOUN
ajlm-269	92	9	infections	infection	NOUN
ajlm-269	92	10	,	,	PUNCT
ajlm-269	92	11	which	which	PRON
ajlm-269	92	12	generally	generally	ADV
ajlm-269	92	13	have	have	VERB
ajlm-269	92	14	viral	viral	ADJ
ajlm-269	92	15	loads	load	NOUN
ajlm-269	92	16	less	less	ADJ
ajlm-269	92	17	than	than	ADP
ajlm-269	92	18	105	105	NUM
ajlm-269	92	19	iu	iu	NOUN
ajlm-269	92	20	/	/	SYM
ajlm-269	92	21	ml.33	ml.33	VERB
ajlm-269	92	22	the	the	DET
ajlm-269	92	23	detection	detection	NOUN
ajlm-269	92	24	of	of	ADP
ajlm-269	92	25	amplified	amplified	ADJ
ajlm-269	92	26	nucleic	nucleic	ADJ
ajlm-269	92	27	acid	acid	NOUN
ajlm-269	92	28	using	use	VERB
ajlm-269	92	29	sybr	sybr	ADV
ajlm-269	93	1	green	green	PROPN
ajlm-269	94	1	i	i	PRON
ajlm-269	94	2	is	be	AUX
ajlm-269	94	3	inexpensive	inexpensive	ADJ
ajlm-269	94	4	and	and	CCONJ
ajlm-269	94	5	is	be	AUX
ajlm-269	94	6	an	an	DET
ajlm-269	94	7	easier	easy	ADJ
ajlm-269	94	8	technical	technical	ADJ
ajlm-269	94	9	approach	approach	NOUN
ajlm-269	94	10	in	in	ADP
ajlm-269	94	11	comparison	comparison	NOUN
ajlm-269	94	12	to	to	ADP
ajlm-269	94	13	other	other	ADJ
ajlm-269	94	14	real	real	ADJ
ajlm-269	94	15	-	-	PUNCT
ajlm-269	94	16	time	time	NOUN
ajlm-269	94	17	pcr	pcr	NOUN
ajlm-269	94	18	formats	format	NOUN
ajlm-269	94	19	such	such	ADJ
ajlm-269	94	20	as	as	ADP
ajlm-269	94	21	taqman	taqman	NOUN
ajlm-269	94	22	and	and	CCONJ
ajlm-269	94	23	molecular	molecular	ADJ
ajlm-269	94	24	beacon	beacon	NOUN
ajlm-269	94	25	detection	detection	NOUN
ajlm-269	94	26	methods.14	methods.14	NOUN
ajlm-269	94	27	it	it	PRON
ajlm-269	94	28	is	be	AUX
ajlm-269	94	29	possible	possible	ADJ
ajlm-269	94	30	that	that	SCONJ
ajlm-269	94	31	hcv	hcv	PROPN
ajlm-269	94	32	rna	rna	NOUN
ajlm-269	94	33	viral	viral	ADJ
ajlm-269	94	34	load	load	NOUN
ajlm-269	94	35	in	in	ADP
ajlm-269	94	36	our	our	PRON
ajlm-269	94	37	infant	infant	NOUN
ajlm-269	94	38	dbs	dbs	NOUN
ajlm-269	94	39	specimens	specimen	NOUN
ajlm-269	94	40	was	be	AUX
ajlm-269	94	41	below	below	ADP
ajlm-269	94	42	the	the	DET
ajlm-269	94	43	level	level	NOUN
ajlm-269	94	44	of	of	ADP
ajlm-269	94	45	detection	detection	NOUN
ajlm-269	94	46	of	of	ADP
ajlm-269	94	47	the	the	DET
ajlm-269	94	48	assay	assay	NOUN
ajlm-269	94	49	,	,	PUNCT
ajlm-269	94	50	since	since	SCONJ
ajlm-269	94	51	hcv	hcv	PROPN
ajlm-269	94	52	rna	rna	VERB
ajlm-269	94	53	viral	viral	ADJ
ajlm-269	94	54	loads	load	NOUN
ajlm-269	94	55	amongst	amongst	ADP
ajlm-269	94	56	infants	infant	NOUN
ajlm-269	94	57	in	in	ADP
ajlm-269	94	58	the	the	DET
ajlm-269	94	59	first	first	ADJ
ajlm-269	94	60	year	year	NOUN
ajlm-269	94	61	of	of	ADP
ajlm-269	94	62	infection	infection	NOUN
ajlm-269	94	63	may	may	AUX
ajlm-269	94	64	be	be	AUX
ajlm-269	94	65	too	too	ADV
ajlm-269	94	66	low	low	ADJ
ajlm-269	94	67	for	for	SCONJ
ajlm-269	94	68	detection.3	detection.3	PROPN
ajlm-269	94	69	dbs	dbs	PROPN
ajlm-269	94	70	specimens	specimen	NOUN
ajlm-269	94	71	have	have	AUX
ajlm-269	94	72	improved	improve	VERB
ajlm-269	94	73	the	the	DET
ajlm-269	94	74	cost	cost	NOUN
ajlm-269	94	75	-	-	PUNCT
ajlm-269	94	76	effectiveness	effectiveness	NOUN
ajlm-269	94	77	,	,	PUNCT
ajlm-269	94	78	coverage	coverage	NOUN
ajlm-269	94	79	and	and	CCONJ
ajlm-269	94	80	availability	availability	NOUN
ajlm-269	94	81	of	of	ADP
ajlm-269	94	82	molecular	molecular	NOUN
ajlm-269	94	83	-	-	PUNCT
ajlm-269	94	84	based	base	VERB
ajlm-269	94	85	testing	testing	NOUN
ajlm-269	94	86	in	in	ADP
ajlm-269	94	87	resource	resource	NOUN
ajlm-269	94	88	-	-	PUNCT
ajlm-269	94	89	limited	limit	VERB
ajlm-269	94	90	settings.11,24,34,35	settings.11,24,34,35	NOUN
ajlm-269	94	91	this	this	PRON
ajlm-269	94	92	has	have	AUX
ajlm-269	94	93	resulted	result	VERB
ajlm-269	94	94	in	in	ADP
ajlm-269	94	95	the	the	DET
ajlm-269	94	96	development	development	NOUN
ajlm-269	94	97	of	of	ADP
ajlm-269	94	98	several	several	ADJ
ajlm-269	94	99	optimised	optimise	VERB
ajlm-269	94	100	pcr	pcr	NOUN
ajlm-269	94	101	techniques	technique	NOUN
ajlm-269	94	102	to	to	PART
ajlm-269	94	103	detect	detect	VERB
ajlm-269	94	104	hcv	hcv	NOUN
ajlm-269	94	105	using	use	VERB
ajlm-269	94	106	dbs.21,22,24,25	dbs.21,22,24,25	PROPN
ajlm-269	94	107	as	as	ADP
ajlm-269	94	108	with	with	ADP
ajlm-269	94	109	our	our	PRON
ajlm-269	94	110	method	method	NOUN
ajlm-269	94	111	,	,	PUNCT
ajlm-269	94	112	several	several	ADJ
ajlm-269	94	113	of	of	ADP
ajlm-269	94	114	these	these	DET
ajlm-269	94	115	techniques	technique	NOUN
ajlm-269	94	116	are	be	AUX
ajlm-269	94	117	based	base	VERB
ajlm-269	94	118	on	on	ADP
ajlm-269	94	119	real	real	ADJ
ajlm-269	94	120	-	-	PUNCT
ajlm-269	94	121	time	time	NOUN
ajlm-269	94	122	pcr	pcr	NOUN
ajlm-269	94	123	and	and	CCONJ
ajlm-269	94	124	,	,	PUNCT
ajlm-269	94	125	like	like	INTJ
ajlm-269	94	126	de	de	X
ajlm-269	94	127	crignis,22	crignis,22	X
ajlm-269	94	128	we	we	PRON
ajlm-269	94	129	used	use	VERB
ajlm-269	94	130	lightcycler	lightcycler	NOUN
ajlm-269	94	131	®	®	NOUN
ajlm-269	94	132	technology	technology	NOUN
ajlm-269	94	133	with	with	ADP
ajlm-269	94	134	sybr	sybr	PROPN
ajlm-269	94	135	green	green	PROPN
ajlm-269	94	136	i	i	PROPN
ajlm-269	94	137	as	as	SCONJ
ajlm-269	94	138	the	the	DET
ajlm-269	94	139	fluorophore.21,24,25	fluorophore.21,24,25	PUNCT
ajlm-269	94	140	probe	probe	NOUN
ajlm-269	94	141	-	-	PUNCT
ajlm-269	94	142	based	base	VERB
ajlm-269	94	143	detection	detection	NOUN
ajlm-269	94	144	methods	method	NOUN
ajlm-269	94	145	are	be	AUX
ajlm-269	94	146	known	know	VERB
ajlm-269	94	147	to	to	PART
ajlm-269	94	148	improve	improve	VERB
ajlm-269	94	149	the	the	DET
ajlm-269	94	150	sensitivity	sensitivity	NOUN
ajlm-269	94	151	of	of	ADP
ajlm-269	94	152	target	target	NOUN
ajlm-269	94	153	detection	detection	NOUN
ajlm-269	94	154	,	,	PUNCT
ajlm-269	94	155	but	but	CCONJ
ajlm-269	94	156	increase	increase	VERB
ajlm-269	94	157	the	the	DET
ajlm-269	94	158	cost	cost	NOUN
ajlm-269	94	159	of	of	ADP
ajlm-269	94	160	the	the	DET
ajlm-269	94	161	testing.21	testing.21	NOUN
ajlm-269	94	162	the	the	DET
ajlm-269	94	163	limit	limit	NOUN
ajlm-269	94	164	of	of	ADP
ajlm-269	94	165	detection	detection	NOUN
ajlm-269	94	166	varies	vary	VERB
ajlm-269	94	167	amongst	amongst	ADP
ajlm-269	94	168	assays	assay	NOUN
ajlm-269	94	169	.	.	PUNCT
ajlm-269	95	1	our	our	PRON
ajlm-269	95	2	assay	assay	NOUN
ajlm-269	95	3	had	have	VERB
ajlm-269	95	4	a	a	DET
ajlm-269	95	5	limit	limit	NOUN
ajlm-269	95	6	of	of	ADP
ajlm-269	95	7	detection	detection	NOUN
ajlm-269	95	8	of	of	ADP
ajlm-269	95	9	3580	3580	NUM
ajlm-269	95	10	iu	iu	NOUN
ajlm-269	95	11	/	/	SYM
ajlm-269	95	12	ml	ml	NOUN
ajlm-269	95	13	,	,	PUNCT
ajlm-269	95	14	which	which	PRON
ajlm-269	95	15	is	be	AUX
ajlm-269	95	16	higher	high	ADJ
ajlm-269	95	17	than	than	SCONJ
ajlm-269	95	18	previously	previously	ADV
ajlm-269	95	19	reported	report	VERB
ajlm-269	95	20	limits	limit	NOUN
ajlm-269	95	21	of	of	ADP
ajlm-269	95	22	detection	detection	NOUN
ajlm-269	95	23	of	of	ADP
ajlm-269	95	24	2500	2500	NUM
ajlm-269	95	25	iu	iu	NOUN
ajlm-269	95	26	/	/	SYM
ajlm-269	95	27	ml22	ml22	PROPN
ajlm-269	95	28	and	and	CCONJ
ajlm-269	95	29	250	250	NUM
ajlm-269	95	30	iu	iu	NOUN
ajlm-269	95	31	/	/	SYM
ajlm-269	95	32	ml.21	ml.21	NOUN
ajlm-269	95	33	this	this	DET
ajlm-269	95	34	difference	difference	NOUN
ajlm-269	95	35	may	may	AUX
ajlm-269	95	36	be	be	AUX
ajlm-269	95	37	attributed	attribute	VERB
ajlm-269	95	38	to	to	ADP
ajlm-269	95	39	differences	difference	NOUN
ajlm-269	95	40	in	in	ADP
ajlm-269	95	41	storage	storage	NOUN
ajlm-269	95	42	conditions	condition	NOUN
ajlm-269	95	43	of	of	ADP
ajlm-269	95	44	the	the	DET
ajlm-269	95	45	dbs	dbs	NOUN
ajlm-269	95	46	specimens	specimen	NOUN
ajlm-269	95	47	before	before	ADP
ajlm-269	95	48	testing	testing	NOUN
ajlm-269	95	49	and	and	CCONJ
ajlm-269	95	50	differences	difference	NOUN
ajlm-269	95	51	in	in	ADP
ajlm-269	95	52	methods	method	NOUN
ajlm-269	95	53	of	of	ADP
ajlm-269	95	54	rna	rna	NOUN
ajlm-269	95	55	extraction	extraction	NOUN
ajlm-269	95	56	,	,	PUNCT
ajlm-269	95	57	which	which	PRON
ajlm-269	95	58	may	may	AUX
ajlm-269	95	59	result	result	VERB
ajlm-269	95	60	in	in	ADP
ajlm-269	95	61	variations	variation	NOUN
ajlm-269	95	62	in	in	ADP
ajlm-269	95	63	the	the	DET
ajlm-269	95	64	number	number	NOUN
ajlm-269	95	65	of	of	ADP
ajlm-269	95	66	copies	copy	NOUN
ajlm-269	95	67	of	of	ADP
ajlm-269	95	68	starting	start	VERB
ajlm-269	95	69	rna.11,25,34	rna.11,25,34	PROPN
ajlm-269	95	70	additionally	additionally	ADV
ajlm-269	95	71	,	,	PUNCT
ajlm-269	95	72	dbs	dbs	ADJ
ajlm-269	95	73	specimen	speciman	NOUN
ajlm-269	95	74	volumes	volume	NOUN
ajlm-269	95	75	are	be	AUX
ajlm-269	95	76	lower	low	ADJ
ajlm-269	95	77	when	when	SCONJ
ajlm-269	95	78	compared	compare	VERB
ajlm-269	95	79	with	with	ADP
ajlm-269	95	80	plasma	plasma	NOUN
ajlm-269	95	81	and	and	CCONJ
ajlm-269	95	82	serum	serum	NOUN
ajlm-269	95	83	specimens	specimen	NOUN
ajlm-269	95	84	,	,	PUNCT
ajlm-269	95	85	which	which	PRON
ajlm-269	95	86	reduces	reduce	VERB
ajlm-269	95	87	the	the	DET
ajlm-269	95	88	amount	amount	NOUN
ajlm-269	95	89	of	of	ADP
ajlm-269	95	90	rna	rna	NOUN
ajlm-269	95	91	extracted.34	extracted.34	NOUN
ajlm-269	95	92	thus	thus	ADV
ajlm-269	95	93	,	,	PUNCT
ajlm-269	95	94	the	the	DET
ajlm-269	95	95	correlation	correlation	NOUN
ajlm-269	95	96	between	between	ADP
ajlm-269	95	97	hcv	hcv	X
ajlm-269	95	98	viral	viral	ADJ
ajlm-269	95	99	load	load	NOUN
ajlm-269	95	100	from	from	ADP
ajlm-269	95	101	dbs	dbs	PROPN
ajlm-269	95	102	and	and	CCONJ
ajlm-269	95	103	hcv	hcv	VERB
ajlm-269	95	104	viral	viral	ADJ
ajlm-269	95	105	load	load	NOUN
ajlm-269	95	106	from	from	ADP
ajlm-269	95	107	plasma	plasma	NOUN
ajlm-269	95	108	or	or	CCONJ
ajlm-269	95	109	serum	serum	NOUN
ajlm-269	95	110	may	may	AUX
ajlm-269	95	111	be	be	AUX
ajlm-269	95	112	imprecise	imprecise	ADV
ajlm-269	95	113	,	,	PUNCT
ajlm-269	95	114	suggesting	suggest	VERB
ajlm-269	95	115	that	that	SCONJ
ajlm-269	95	116	dbs	dbs	PROPN
ajlm-269	95	117	specimens	specimen	NOUN
ajlm-269	95	118	are	be	AUX
ajlm-269	95	119	better	well	ADV
ajlm-269	95	120	suited	suited	ADJ
ajlm-269	95	121	to	to	ADP
ajlm-269	95	122	diagnosis	diagnosis	NOUN
ajlm-269	95	123	,	,	PUNCT
ajlm-269	95	124	whereas	whereas	SCONJ
ajlm-269	95	125	plasma	plasma	NOUN
ajlm-269	95	126	or	or	CCONJ
ajlm-269	95	127	serum	serum	NOUN
ajlm-269	95	128	are	be	AUX
ajlm-269	95	129	better	well	ADV
ajlm-269	95	130	suited	suited	ADJ
ajlm-269	95	131	to	to	ADP
ajlm-269	95	132	monitoring	monitoring	NOUN
ajlm-269	95	133	of	of	ADP
ajlm-269	95	134	hcv	hcv	ADJ
ajlm-269	95	135	antiviral	antiviral	ADJ
ajlm-269	95	136	treatment.34	treatment.34	NOUN
ajlm-269	95	137	these	these	DET
ajlm-269	95	138	methods	method	NOUN
ajlm-269	95	139	for	for	ADP
ajlm-269	95	140	hcv	hcv	NOUN
ajlm-269	95	141	testing	testing	NOUN
ajlm-269	95	142	using	use	VERB
ajlm-269	95	143	real	real	ADJ
ajlm-269	95	144	-	-	PUNCT
ajlm-269	95	145	time	time	NOUN
ajlm-269	95	146	pcr	pcr	NOUN
ajlm-269	95	147	have	have	AUX
ajlm-269	95	148	been	be	AUX
ajlm-269	95	149	applied	apply	VERB
ajlm-269	95	150	to	to	AUX
ajlm-269	95	151	hcv	hcv	VERB
ajlm-269	95	152	treatment	treatment	NOUN
ajlm-269	95	153	monitoring,24	monitoring,24	NOUN
ajlm-269	95	154	screening	screen	VERB
ajlm-269	95	155	for	for	ADP
ajlm-269	95	156	hiv	hiv	PROPN
ajlm-269	95	157	/	/	SYM
ajlm-269	95	158	hcv	hcv	X
ajlm-269	95	159	co	co	NOUN
ajlm-269	95	160	-	-	NOUN
ajlm-269	95	161	infection22	infection22	NOUN
ajlm-269	95	162	and	and	CCONJ
ajlm-269	95	163	surveillance	surveillance	NOUN
ajlm-269	95	164	for	for	ADP
ajlm-269	95	165	hcv	hcv	PROPN
ajlm-269	95	166	amongst	amongst	ADP
ajlm-269	95	167	intravenous	intravenous	ADJ
ajlm-269	95	168	drug	drug	NOUN
ajlm-269	95	169	users.36	users.36	NOUN
ajlm-269	95	170	our	our	PRON
ajlm-269	95	171	real	real	ADJ
ajlm-269	95	172	-	-	PUNCT
ajlm-269	95	173	time	time	NOUN
ajlm-269	95	174	hcv	hcv	NOUN
ajlm-269	95	175	pcr	pcr	NOUN
ajlm-269	95	176	was	be	AUX
ajlm-269	95	177	used	use	VERB
ajlm-269	95	178	on	on	ADP
ajlm-269	95	179	dbs	dbs	PROPN
ajlm-269	95	180	specimens	specimen	NOUN
ajlm-269	95	181	from	from	ADP
ajlm-269	95	182	an	an	DET
ajlm-269	95	183	infant	infant	NOUN
ajlm-269	95	184	population	population	NOUN
ajlm-269	95	185	,	,	PUNCT
ajlm-269	95	186	which	which	PRON
ajlm-269	95	187	may	may	AUX
ajlm-269	95	188	be	be	AUX
ajlm-269	95	189	useful	useful	ADJ
ajlm-269	95	190	for	for	ADP
ajlm-269	95	191	hcv	hcv	NOUN
ajlm-269	95	192	surveillance	surveillance	NOUN
ajlm-269	95	193	amongst	amongst	ADP
ajlm-269	95	194	infants	infant	NOUN
ajlm-269	95	195	.	.	PUNCT
ajlm-269	96	1	the	the	DET
ajlm-269	96	2	use	use	NOUN
ajlm-269	96	3	of	of	ADP
ajlm-269	96	4	dbs	dbs	PROPN
ajlm-269	96	5	specimens	specimen	NOUN
ajlm-269	96	6	for	for	ADP
ajlm-269	96	7	hcv	hcv	X
ajlm-269	96	8	pcr	pcr	PROPN
ajlm-269	96	9	has	have	VERB
ajlm-269	96	10	several	several	ADJ
ajlm-269	96	11	advantages.11,34,35	advantages.11,34,35	X
ajlm-269	96	12	firstly	firstly	ADV
ajlm-269	96	13	,	,	PUNCT
ajlm-269	96	14	they	they	PRON
ajlm-269	96	15	are	be	AUX
ajlm-269	96	16	easier	easy	ADJ
ajlm-269	96	17	to	to	PART
ajlm-269	96	18	collect	collect	VERB
ajlm-269	96	19	than	than	ADP
ajlm-269	96	20	whole	whole	ADJ
ajlm-269	96	21	blood	blood	NOUN
ajlm-269	96	22	and	and	CCONJ
ajlm-269	96	23	present	present	VERB
ajlm-269	96	24	a	a	DET
ajlm-269	96	25	lower	low	ADJ
ajlm-269	96	26	infectious	infectious	ADJ
ajlm-269	96	27	hazard	hazard	NOUN
ajlm-269	96	28	.	.	PUNCT
ajlm-269	97	1	secondly	secondly	ADV
ajlm-269	97	2	,	,	PUNCT
ajlm-269	97	3	specimen	speciman	NOUN
ajlm-269	97	4	transportation	transportation	NOUN
ajlm-269	97	5	and	and	CCONJ
ajlm-269	97	6	storage	storage	NOUN
ajlm-269	97	7	are	be	AUX
ajlm-269	97	8	cheaper	cheap	ADJ
ajlm-269	97	9	,	,	PUNCT
ajlm-269	97	10	since	since	SCONJ
ajlm-269	97	11	refrigeration	refrigeration	NOUN
ajlm-269	97	12	is	be	AUX
ajlm-269	97	13	not	not	PART
ajlm-269	97	14	required	require	VERB
ajlm-269	97	15	.	.	PUNCT
ajlm-269	98	1	lastly	lastly	ADV
ajlm-269	98	2	,	,	PUNCT
ajlm-269	98	3	the	the	DET
ajlm-269	98	4	long	long	ADJ
ajlm-269	98	5	-	-	PUNCT
ajlm-269	98	6	term	term	NOUN
ajlm-269	98	7	stability	stability	NOUN
ajlm-269	98	8	of	of	ADP
ajlm-269	98	9	nucleic	nucleic	ADJ
ajlm-269	98	10	acids	acid	NOUN
ajlm-269	98	11	in	in	ADP
ajlm-269	98	12	dbs	dbs	PROPN
ajlm-269	98	13	has	have	AUX
ajlm-269	98	14	been	be	AUX
ajlm-269	98	15	proven	prove	VERB
ajlm-269	98	16	for	for	ADP
ajlm-269	98	17	molecular	molecular	ADJ
ajlm-269	98	18	analysis	analysis	NOUN
ajlm-269	98	19	.	.	PUNCT
ajlm-269	99	1	therefore	therefore	ADV
ajlm-269	99	2	,	,	PUNCT
ajlm-269	99	3	real	real	ADJ
ajlm-269	99	4	-	-	PUNCT
ajlm-269	99	5	time	time	NOUN
ajlm-269	99	6	hcv	hcv	NOUN
ajlm-269	99	7	pcr	pcr	NOUN
ajlm-269	99	8	using	use	VERB
ajlm-269	99	9	dbs	dbs	NOUN
ajlm-269	99	10	specimens	specimen	NOUN
ajlm-269	99	11	for	for	ADP
ajlm-269	99	12	hcv	hcv	NOUN
ajlm-269	99	13	surveillance	surveillance	NOUN
ajlm-269	99	14	has	have	AUX
ajlm-269	99	15	been	be	AUX
ajlm-269	99	16	used	use	VERB
ajlm-269	99	17	to	to	PART
ajlm-269	99	18	understand	understand	VERB
ajlm-269	99	19	the	the	DET
ajlm-269	99	20	molecular	molecular	ADJ
ajlm-269	99	21	epidemiology	epidemiology	NOUN
ajlm-269	99	22	of	of	ADP
ajlm-269	99	23	hcv.21,22,23,24,25,36	hcv.21,22,23,24,25,36	PROPN
ajlm-269	99	24	limitations	limitation	NOUN
ajlm-269	99	25	our	our	PRON
ajlm-269	99	26	hcv	hcv	PROPN
ajlm-269	99	27	pcr	pcr	ADJ
ajlm-269	99	28	optimisation	optimisation	NOUN
ajlm-269	99	29	has	have	VERB
ajlm-269	99	30	several	several	ADJ
ajlm-269	99	31	limitations	limitation	NOUN
ajlm-269	99	32	.	.	PUNCT
ajlm-269	100	1	firstly	firstly	ADV
ajlm-269	100	2	,	,	PUNCT
ajlm-269	100	3	the	the	DET
ajlm-269	100	4	results	result	NOUN
ajlm-269	100	5	are	be	AUX
ajlm-269	100	6	not	not	PART
ajlm-269	100	7	representative	representative	ADJ
ajlm-269	100	8	of	of	ADP
ajlm-269	100	9	all	all	DET
ajlm-269	100	10	infants	infant	NOUN
ajlm-269	100	11	,	,	PUNCT
ajlm-269	100	12	since	since	SCONJ
ajlm-269	100	13	only	only	ADV
ajlm-269	100	14	specimens	specimen	NOUN
ajlm-269	100	15	from	from	ADP
ajlm-269	100	16	hiv	hiv	PROPN
ajlm-269	100	17	-	-	PUNCT
ajlm-269	100	18	exposed	expose	VERB
ajlm-269	100	19	infants	infant	NOUN
ajlm-269	100	20	who	who	PRON
ajlm-269	100	21	require	require	VERB
ajlm-269	100	22	an	an	DET
ajlm-269	100	23	hiv	hiv	PROPN
ajlm-269	100	24	pcr	pcr	PROPN
ajlm-269	100	25	were	be	AUX
ajlm-269	100	26	tested	test	VERB
ajlm-269	100	27	.	.	PUNCT
ajlm-269	101	1	secondly	secondly	ADV
ajlm-269	101	2	,	,	PUNCT
ajlm-269	101	3	our	our	PRON
ajlm-269	101	4	optimised	optimise	VERB
ajlm-269	101	5	hcv	hcv	NOUN
ajlm-269	101	6	pcr	pcr	NOUN
ajlm-269	101	7	may	may	AUX
ajlm-269	101	8	not	not	PART
ajlm-269	101	9	be	be	AUX
ajlm-269	101	10	optimal	optimal	ADJ
ajlm-269	101	11	for	for	ADP
ajlm-269	101	12	routine	routine	ADJ
ajlm-269	101	13	clinical	clinical	ADJ
ajlm-269	101	14	diagnostic	diagnostic	ADJ
ajlm-269	101	15	purposes	purpose	NOUN
ajlm-269	101	16	,	,	PUNCT
ajlm-269	101	17	although	although	SCONJ
ajlm-269	101	18	it	it	PRON
ajlm-269	101	19	may	may	AUX
ajlm-269	101	20	be	be	AUX
ajlm-269	101	21	convenient	convenient	ADJ
ajlm-269	101	22	for	for	ADP
ajlm-269	101	23	surveillance	surveillance	NOUN
ajlm-269	101	24	work	work	NOUN
ajlm-269	101	25	.	.	PUNCT
ajlm-269	102	1	thirdly	thirdly	ADV
ajlm-269	102	2	,	,	PUNCT
ajlm-269	102	3	we	we	PRON
ajlm-269	102	4	did	do	AUX
ajlm-269	102	5	not	not	PART
ajlm-269	102	6	compare	compare	VERB
ajlm-269	102	7	our	our	PRON
ajlm-269	102	8	dbs	dbs	PROPN
ajlm-269	102	9	specimen	speciman	NOUN
ajlm-269	102	10	method	method	VERB
ajlm-269	102	11	to	to	ADP
ajlm-269	102	12	those	those	PRON
ajlm-269	102	13	using	use	VERB
ajlm-269	102	14	whole	whole	ADJ
ajlm-269	102	15	blood	blood	NOUN
ajlm-269	102	16	.	.	PUNCT
ajlm-269	103	1	however	however	ADV
ajlm-269	103	2	,	,	PUNCT
ajlm-269	103	3	we	we	PRON
ajlm-269	103	4	did	do	AUX
ajlm-269	103	5	use	use	VERB
ajlm-269	103	6	alternative	alternative	ADJ
ajlm-269	103	7	methods	method	NOUN
ajlm-269	103	8	to	to	PART
ajlm-269	103	9	evaluate	evaluate	VERB
ajlm-269	103	10	our	our	PRON
ajlm-269	103	11	pcr	pcr	NOUN
ajlm-269	103	12	assay	assay	NOUN
ajlm-269	103	13	,	,	PUNCT
ajlm-269	103	14	which	which	PRON
ajlm-269	103	15	included	include	VERB
ajlm-269	103	16	testing	test	VERB
ajlm-269	103	17	external	external	ADJ
ajlm-269	103	18	quality	quality	NOUN
ajlm-269	103	19	control	control	NOUN
ajlm-269	103	20	specimens	specimen	NOUN
ajlm-269	103	21	with	with	ADP
ajlm-269	103	22	known	know	VERB
ajlm-269	103	23	hcv	hcv	NOUN
ajlm-269	103	24	viral	viral	ADJ
ajlm-269	103	25	loads	load	NOUN
ajlm-269	103	26	ranging	range	VERB
ajlm-269	103	27	from	from	ADP
ajlm-269	103	28	1081	1081	NUM
ajlm-269	103	29	iu	iu	NOUN
ajlm-269	103	30	/	/	SYM
ajlm-269	103	31	ml	ml	NOUN
ajlm-269	103	32	to	to	ADP
ajlm-269	103	33	34	34	NUM
ajlm-269	103	34	594	594	NUM
ajlm-269	103	35	iu	iu	NOUN
ajlm-269	103	36	/	/	SYM
ajlm-269	103	37	ml	ml	ADJ
ajlm-269	103	38	and	and	CCONJ
ajlm-269	103	39	clinical	clinical	ADJ
ajlm-269	103	40	hcv	hcv	NOUN
ajlm-269	103	41	-	-	PUNCT
ajlm-269	103	42	positive	positive	ADJ
ajlm-269	103	43	and	and	CCONJ
ajlm-269	103	44	hcv	hcv	NOUN
ajlm-269	103	45	-	-	PUNCT
ajlm-269	103	46	negative	negative	ADJ
ajlm-269	103	47	specimens	specimen	NOUN
ajlm-269	103	48	from	from	ADP
ajlm-269	103	49	a	a	DET
ajlm-269	103	50	reference	reference	NOUN
ajlm-269	103	51	laboratory	laboratory	NOUN
ajlm-269	103	52	.	.	PUNCT
ajlm-269	104	1	further	far	ADV
ajlm-269	104	2	,	,	PUNCT
ajlm-269	104	3	positive	positive	ADJ
ajlm-269	104	4	and	and	CCONJ
ajlm-269	104	5	negative	negative	ADJ
ajlm-269	104	6	controls	control	NOUN
ajlm-269	104	7	were	be	AUX
ajlm-269	104	8	included	include	VERB
ajlm-269	104	9	in	in	ADP
ajlm-269	104	10	each	each	DET
ajlm-269	104	11	run	run	NOUN
ajlm-269	104	12	.	.	PUNCT
ajlm-269	105	1	lastly	lastly	ADV
ajlm-269	105	2	,	,	PUNCT
ajlm-269	105	3	we	we	PRON
ajlm-269	105	4	chose	choose	VERB
ajlm-269	105	5	not	not	PART
ajlm-269	105	6	to	to	PART
ajlm-269	105	7	follow	follow	VERB
ajlm-269	105	8	previously	previously	ADV
ajlm-269	105	9	described	describe	VERB
ajlm-269	105	10	methods	method	NOUN
ajlm-269	105	11	but	but	CCONJ
ajlm-269	105	12	to	to	PART
ajlm-269	105	13	optimise	optimise	VERB
ajlm-269	105	14	our	our	PRON
ajlm-269	105	15	own	own	ADJ
ajlm-269	105	16	method	method	NOUN
ajlm-269	105	17	,	,	PUNCT
ajlm-269	105	18	because	because	SCONJ
ajlm-269	105	19	it	it	PRON
ajlm-269	105	20	was	be	AUX
ajlm-269	105	21	more	more	ADV
ajlm-269	105	22	cost	cost	NOUN
ajlm-269	105	23	-	-	PUNCT
ajlm-269	105	24	effective	effective	ADJ
ajlm-269	105	25	than	than	ADP
ajlm-269	105	26	other	other	ADJ
ajlm-269	105	27	real	real	ADJ
ajlm-269	105	28	-	-	PUNCT
ajlm-269	105	29	time	time	NOUN
ajlm-269	105	30	techniques	technique	NOUN
ajlm-269	105	31	reported	report	VERB
ajlm-269	105	32	.	.	PUNCT
ajlm-269	106	1	in	in	ADP
ajlm-269	106	2	addition	addition	NOUN
ajlm-269	106	3	,	,	PUNCT
ajlm-269	106	4	we	we	PRON
ajlm-269	106	5	used	use	VERB
ajlm-269	106	6	existing	exist	VERB
ajlm-269	106	7	equipment	equipment	NOUN
ajlm-269	106	8	and	and	CCONJ
ajlm-269	106	9	in	in	ADP
ajlm-269	106	10	-	-	PUNCT
ajlm-269	106	11	house	house	NOUN
ajlm-269	106	12	pcr	pcr	NOUN
ajlm-269	106	13	consumables	consumable	NOUN
ajlm-269	106	14	that	that	PRON
ajlm-269	106	15	the	the	DET
ajlm-269	106	16	technical	technical	ADJ
ajlm-269	106	17	staff	staff	NOUN
ajlm-269	106	18	in	in	ADP
ajlm-269	106	19	our	our	PRON
ajlm-269	106	20	laboratory	laboratory	NOUN
ajlm-269	106	21	were	be	AUX
ajlm-269	106	22	already	already	ADV
ajlm-269	106	23	proficient	proficient	ADJ
ajlm-269	106	24	in	in	ADP
ajlm-269	106	25	using	use	VERB
ajlm-269	106	26	.	.	PUNCT
ajlm-269	107	1	given	give	VERB
ajlm-269	107	2	that	that	SCONJ
ajlm-269	107	3	our	our	PRON
ajlm-269	107	4	dbs	dbs	NOUN
ajlm-269	107	5	specimens	specimen	NOUN
ajlm-269	107	6	were	be	AUX
ajlm-269	107	7	from	from	ADP
ajlm-269	107	8	hiv	hiv	PROPN
ajlm-269	107	9	-	-	PUNCT
ajlm-269	107	10	exposed	expose	VERB
ajlm-269	107	11	infants	infant	NOUN
ajlm-269	107	12	in	in	ADP
ajlm-269	107	13	kwazulu	kwazulu	NOUN
ajlm-269	107	14	-	-	PUNCT
ajlm-269	107	15	natal	natal	NOUN
ajlm-269	107	16	,	,	PUNCT
ajlm-269	107	17	we	we	PRON
ajlm-269	107	18	expected	expect	VERB
ajlm-269	107	19	to	to	PART
ajlm-269	107	20	find	find	VERB
ajlm-269	107	21	a	a	DET
ajlm-269	107	22	moderate	moderate	ADJ
ajlm-269	107	23	prevalence	prevalence	NOUN
ajlm-269	107	24	of	of	ADP
ajlm-269	107	25	hcv	hcv	PROPN
ajlm-269	107	26	amongst	amongst	ADP
ajlm-269	107	27	these	these	DET
ajlm-269	107	28	infants	infant	NOUN
ajlm-269	107	29	as	as	SCONJ
ajlm-269	107	30	reported	report	VERB
ajlm-269	107	31	by	by	ADP
ajlm-269	107	32	other	other	ADJ
ajlm-269	107	33	african	african	ADJ
ajlm-269	107	34	countries	country	NOUN
ajlm-269	107	35	with	with	ADP
ajlm-269	107	36	populations	population	NOUN
ajlm-269	107	37	co	co	VERB
ajlm-269	107	38	-	-	VERB
ajlm-269	107	39	infected	infected	ADJ
ajlm-269	107	40	with	with	ADP
ajlm-269	107	41	hiv	hiv	PROPN
ajlm-269	107	42	and	and	CCONJ
ajlm-269	107	43	hcv.37,38,39	hcv.37,38,39	PROPN
ajlm-269	107	44	infection	infection	NOUN
ajlm-269	107	45	with	with	ADP
ajlm-269	107	46	hiv	hiv	PROPN
ajlm-269	107	47	is	be	AUX
ajlm-269	107	48	a	a	DET
ajlm-269	107	49	risk	risk	NOUN
ajlm-269	107	50	factor	factor	NOUN
ajlm-269	107	51	for	for	ADP
ajlm-269	107	52	hcv	hcv	NOUN
ajlm-269	107	53	transmission	transmission	NOUN
ajlm-269	107	54	in	in	ADP
ajlm-269	107	55	co	co	ADJ
ajlm-269	107	56	-	-	ADJ
ajlm-269	107	57	infected	infected	ADJ
ajlm-269	107	58	pregnant	pregnant	ADJ
ajlm-269	107	59	women	woman	NOUN
ajlm-269	107	60	and	and	CCONJ
ajlm-269	107	61	is	be	AUX
ajlm-269	107	62	known	know	VERB
ajlm-269	107	63	to	to	PART
ajlm-269	107	64	increase	increase	VERB
ajlm-269	107	65	vertical	vertical	ADJ
ajlm-269	107	66	transmission.3,4	transmission.3,4	ADP
ajlm-269	107	67	the	the	DET
ajlm-269	107	68	fact	fact	NOUN
ajlm-269	107	69	that	that	SCONJ
ajlm-269	107	70	we	we	PRON
ajlm-269	107	71	did	do	AUX
ajlm-269	107	72	not	not	PART
ajlm-269	107	73	detect	detect	VERB
ajlm-269	107	74	hcv	hcv	NOUN
ajlm-269	107	75	rna	rna	NOUN
ajlm-269	107	76	in	in	ADP
ajlm-269	107	77	our	our	PRON
ajlm-269	107	78	study	study	NOUN
ajlm-269	107	79	specimens	specimen	NOUN
ajlm-269	107	80	may	may	AUX
ajlm-269	107	81	be	be	AUX
ajlm-269	107	82	because	because	SCONJ
ajlm-269	107	83	of	of	ADP
ajlm-269	107	84	the	the	DET
ajlm-269	107	85	prolonged	prolonged	ADJ
ajlm-269	107	86	storage	storage	NOUN
ajlm-269	107	87	(	(	PUNCT
ajlm-269	107	88	≥	≥	NOUN
ajlm-269	107	89	3	3	NUM
ajlm-269	107	90	months	month	NOUN
ajlm-269	107	91	)	)	PUNCT
ajlm-269	107	92	of	of	ADP
ajlm-269	107	93	our	our	PRON
ajlm-269	107	94	dbs	dbs	NOUN
ajlm-269	107	95	specimens	specimen	NOUN
ajlm-269	107	96	at	at	ADP
ajlm-269	107	97	room	room	NOUN
ajlm-269	107	98	temperature	temperature	NOUN
ajlm-269	107	99	,	,	PUNCT
ajlm-269	107	100	which	which	PRON
ajlm-269	107	101	may	may	AUX
ajlm-269	107	102	have	have	AUX
ajlm-269	107	103	resulted	result	VERB
ajlm-269	107	104	in	in	ADP
ajlm-269	107	105	degradation	degradation	NOUN
ajlm-269	107	106	of	of	ADP
ajlm-269	107	107	hcv	hcv	PROPN
ajlm-269	107	108	rna.25	rna.25	NOUN
ajlm-269	107	109	furthermore	furthermore	ADV
ajlm-269	107	110	,	,	PUNCT
ajlm-269	107	111	perinatal	perinatal	ADJ
ajlm-269	107	112	transmission	transmission	NOUN
ajlm-269	107	113	of	of	ADP
ajlm-269	107	114	hcv	hcv	PROPN
ajlm-269	107	115	requires	require	VERB
ajlm-269	107	116	a	a	DET
ajlm-269	107	117	high	high	ADJ
ajlm-269	107	118	maternal	maternal	ADJ
ajlm-269	107	119	hcv	hcv	NOUN
ajlm-269	107	120	viral	viral	ADJ
ajlm-269	107	121	load.3	load.3	ADJ
ajlm-269	107	122	detection	detection	NOUN
ajlm-269	107	123	of	of	ADP
ajlm-269	107	124	hcv	hcv	PROPN
ajlm-269	107	125	rna	rna	PROPN
ajlm-269	107	126	at	at	ADP
ajlm-269	107	127	birth	birth	NOUN
ajlm-269	107	128	is	be	AUX
ajlm-269	107	129	usually	usually	ADV
ajlm-269	107	130	indicative	indicative	ADJ
ajlm-269	107	131	of	of	ADP
ajlm-269	107	132	intrauterine	intrauterine	ADJ
ajlm-269	107	133	infection	infection	NOUN
ajlm-269	107	134	,	,	PUNCT
ajlm-269	107	135	whereas	whereas	SCONJ
ajlm-269	107	136	infection	infection	NOUN
ajlm-269	107	137	during	during	ADP
ajlm-269	107	138	delivery	delivery	NOUN
ajlm-269	107	139	is	be	AUX
ajlm-269	107	140	associated	associate	VERB
ajlm-269	107	141	with	with	ADP
ajlm-269	107	142	hcv	hcv	PROPN
ajlm-269	107	143	rna	rna	PROPN
ajlm-269	107	144	being	be	AUX
ajlm-269	107	145	detected	detect	VERB
ajlm-269	107	146	only	only	ADV
ajlm-269	107	147	after	after	SCONJ
ajlm-269	107	148	infants	infant	NOUN
ajlm-269	107	149	are	be	AUX
ajlm-269	107	150	aged	age	VERB
ajlm-269	107	151	six	six	NUM
ajlm-269	107	152	months	month	NOUN
ajlm-269	107	153	;	;	PUNCT
ajlm-269	107	154	in	in	ADP
ajlm-269	107	155	addition	addition	NOUN
ajlm-269	107	156	,	,	PUNCT
ajlm-269	107	157	delivery	delivery	NOUN
ajlm-269	107	158	by	by	ADP
ajlm-269	107	159	caesarean	caesarean	ADJ
ajlm-269	107	160	section	section	NOUN
ajlm-269	107	161	is	be	AUX
ajlm-269	107	162	less	less	ADV
ajlm-269	107	163	frequently	frequently	ADV
ajlm-269	107	164	associated	associate	VERB
ajlm-269	107	165	with	with	ADP
ajlm-269	107	166	hcv	hcv	NOUN
ajlm-269	107	167	transmission	transmission	NOUN
ajlm-269	107	168	than	than	ADP
ajlm-269	107	169	vaginal	vaginal	ADJ
ajlm-269	107	170	delivery.3	delivery.3	PROPN
ajlm-269	107	171	however	however	ADV
ajlm-269	107	172	,	,	PUNCT
ajlm-269	107	173	clinical	clinical	ADJ
ajlm-269	107	174	information	information	NOUN
ajlm-269	107	175	,	,	PUNCT
ajlm-269	107	176	such	such	ADJ
ajlm-269	107	177	as	as	ADP
ajlm-269	107	178	maternal	maternal	ADJ
ajlm-269	107	179	hcv	hcv	ADJ
ajlm-269	107	180	viral	viral	ADJ
ajlm-269	107	181	load	load	NOUN
ajlm-269	107	182	,	,	PUNCT
ajlm-269	107	183	mode	mode	NOUN
ajlm-269	107	184	of	of	ADP
ajlm-269	107	185	delivery	delivery	NOUN
ajlm-269	107	186	of	of	ADP
ajlm-269	107	187	infants	infant	NOUN
ajlm-269	107	188	,	,	PUNCT
ajlm-269	107	189	infant	infant	NOUN
ajlm-269	107	190	liver	liver	NOUN
ajlm-269	107	191	function	function	NOUN
ajlm-269	107	192	markers	marker	NOUN
ajlm-269	107	193	,	,	PUNCT
ajlm-269	107	194	risk	risk	NOUN
ajlm-269	107	195	factors	factor	NOUN
ajlm-269	107	196	(	(	PUNCT
ajlm-269	107	197	such	such	ADJ
ajlm-269	107	198	as	as	ADP
ajlm-269	107	199	hbv	hbv	NOUN
ajlm-269	107	200	co	co	NOUN
ajlm-269	107	201	-	-	NOUN
ajlm-269	107	202	infection	infection	NOUN
ajlm-269	107	203	)	)	PUNCT
ajlm-269	107	204	and	and	CCONJ
ajlm-269	107	205	laboratory	laboratory	NOUN
ajlm-269	107	206	results	result	NOUN
ajlm-269	107	207	(	(	PUNCT
ajlm-269	107	208	such	such	ADJ
ajlm-269	107	209	as	as	ADP
ajlm-269	107	210	cd4	cd4	PROPN
ajlm-269	107	211	cell	cell	NOUN
ajlm-269	107	212	counts	count	NOUN
ajlm-269	107	213	and	and	CCONJ
ajlm-269	107	214	hiv	hiv	PROPN
ajlm-269	107	215	viral	viral	ADJ
ajlm-269	107	216	loads	load	NOUN
ajlm-269	107	217	)	)	PUNCT
ajlm-269	107	218	were	be	AUX
ajlm-269	107	219	not	not	PART
ajlm-269	107	220	available	available	ADJ
ajlm-269	107	221	for	for	ADP
ajlm-269	107	222	our	our	PRON
ajlm-269	107	223	study	study	NOUN
ajlm-269	107	224	.	.	PUNCT
ajlm-269	108	1	conclusion	conclusion	NOUN
ajlm-269	108	2	we	we	PRON
ajlm-269	108	3	have	have	AUX
ajlm-269	108	4	demonstrated	demonstrate	VERB
ajlm-269	108	5	that	that	SCONJ
ajlm-269	108	6	using	use	VERB
ajlm-269	108	7	an	an	DET
ajlm-269	108	8	optimised	optimise	VERB
ajlm-269	108	9	real	real	ADJ
ajlm-269	108	10	-	-	PUNCT
ajlm-269	108	11	time	time	NOUN
ajlm-269	108	12	hcv	hcv	X
ajlm-269	108	13	pcr	pcr	NOUN
ajlm-269	108	14	assay	assay	VERB
ajlm-269	108	15	on	on	ADP
ajlm-269	108	16	dbs	dbs	PROPN
ajlm-269	108	17	specimens	specimen	NOUN
ajlm-269	108	18	from	from	ADP
ajlm-269	108	19	hiv	hiv	PROPN
ajlm-269	108	20	-	-	PUNCT
ajlm-269	108	21	exposed	expose	VERB
ajlm-269	108	22	infants	infant	NOUN
ajlm-269	108	23	is	be	AUX
ajlm-269	108	24	a	a	DET
ajlm-269	108	25	good	good	ADJ
ajlm-269	108	26	technique	technique	NOUN
ajlm-269	108	27	for	for	ADP
ajlm-269	108	28	hcv	hcv	NOUN
ajlm-269	108	29	detection	detection	NOUN
ajlm-269	108	30	and	and	CCONJ
ajlm-269	108	31	may	may	AUX
ajlm-269	108	32	be	be	AUX
ajlm-269	108	33	well	well	ADV
ajlm-269	108	34	suited	suit	VERB
ajlm-269	108	35	for	for	ADP
ajlm-269	108	36	surveillance	surveillance	NOUN
ajlm-269	108	37	purposes	purpose	NOUN
ajlm-269	108	38	in	in	ADP
ajlm-269	108	39	resource	resource	NOUN
ajlm-269	108	40	-	-	PUNCT
ajlm-269	108	41	limited	limit	VERB
ajlm-269	108	42	settings	setting	NOUN
ajlm-269	108	43	.	.	PUNCT
ajlm-269	109	1	to	to	ADP
ajlm-269	109	2	our	our	PRON
ajlm-269	109	3	knowledge	knowledge	NOUN
ajlm-269	109	4	,	,	PUNCT
ajlm-269	109	5	this	this	PRON
ajlm-269	109	6	is	be	AUX
ajlm-269	109	7	the	the	DET
ajlm-269	109	8	first	first	ADJ
ajlm-269	109	9	surveillance	surveillance	NOUN
ajlm-269	109	10	in	in	ADP
ajlm-269	109	11	south	south	PROPN
ajlm-269	109	12	africa	africa	PROPN
ajlm-269	109	13	using	use	VERB
ajlm-269	109	14	an	an	DET
ajlm-269	109	15	optimised	optimise	VERB
ajlm-269	109	16	real	real	ADJ
ajlm-269	109	17	-	-	PUNCT
ajlm-269	109	18	time	time	NOUN
ajlm-269	109	19	hcv	hcv	X
ajlm-269	109	20	pcr	pcr	NOUN
ajlm-269	109	21	to	to	PART
ajlm-269	109	22	report	report	VERB
ajlm-269	109	23	the	the	DET
ajlm-269	109	24	prevalence	prevalence	NOUN
ajlm-269	109	25	of	of	ADP
ajlm-269	109	26	hcv	hcv	PROPN
ajlm-269	109	27	amongst	amongst	ADP
ajlm-269	109	28	hiv	hiv	PROPN
ajlm-269	109	29	-	-	PUNCT
ajlm-269	109	30	exposed	expose	VERB
ajlm-269	109	31	infants	infant	NOUN
ajlm-269	109	32	.	.	PUNCT
ajlm-269	110	1	considering	consider	VERB
ajlm-269	110	2	that	that	SCONJ
ajlm-269	110	3	hcv	hcv	NOUN
ajlm-269	110	4	was	be	AUX
ajlm-269	110	5	not	not	PART
ajlm-269	110	6	detected	detect	VERB
ajlm-269	110	7	in	in	ADP
ajlm-269	110	8	our	our	PRON
ajlm-269	110	9	dbs	dbs	NOUN
ajlm-269	110	10	specimens	specimen	NOUN
ajlm-269	110	11	,	,	PUNCT
ajlm-269	110	12	hcv	hcv	VERB
ajlm-269	110	13	prevalence	prevalence	NOUN
ajlm-269	110	14	in	in	ADP
ajlm-269	110	15	this	this	DET
ajlm-269	110	16	population	population	NOUN
ajlm-269	110	17	of	of	ADP
ajlm-269	110	18	infants	infant	NOUN
ajlm-269	110	19	in	in	ADP
ajlm-269	110	20	kwazulu	kwazulu	NOUN
ajlm-269	110	21	-	-	PUNCT
ajlm-269	110	22	natal	natal	ADJ
ajlm-269	110	23	,	,	PUNCT
ajlm-269	110	24	south	south	PROPN
ajlm-269	110	25	africa	africa	PROPN
ajlm-269	110	26	may	may	AUX
ajlm-269	110	27	be	be	AUX
ajlm-269	110	28	low	low	ADJ
ajlm-269	110	29	and	and	CCONJ
ajlm-269	110	30	may	may	AUX
ajlm-269	110	31	have	have	VERB
ajlm-269	110	32	little	little	ADJ
ajlm-269	110	33	impact	impact	NOUN
ajlm-269	110	34	on	on	ADP
ajlm-269	110	35	hiv	hiv	PROPN
ajlm-269	110	36	-	-	PUNCT
ajlm-269	110	37	exposed	expose	VERB
ajlm-269	110	38	infants	infant	NOUN
ajlm-269	110	39	.	.	PUNCT
ajlm-269	111	1	therefore	therefore	ADV
ajlm-269	111	2	,	,	PUNCT
ajlm-269	111	3	further	further	ADJ
ajlm-269	111	4	surveillance	surveillance	NOUN
ajlm-269	111	5	for	for	ADP
ajlm-269	111	6	hcv	hcv	PROPN
ajlm-269	111	7	amongst	amongst	ADP
ajlm-269	111	8	hiv	hiv	PROPN
ajlm-269	111	9	-	-	PUNCT
ajlm-269	111	10	exposed	expose	VERB
ajlm-269	111	11	infants	infant	NOUN
ajlm-269	111	12	in	in	ADP
ajlm-269	111	13	our	our	PRON
ajlm-269	111	14	setting	setting	NOUN
ajlm-269	111	15	may	may	AUX
ajlm-269	111	16	not	not	PART
ajlm-269	111	17	be	be	AUX
ajlm-269	111	18	necessary	necessary	ADJ
ajlm-269	111	19	.	.	PUNCT
ajlm-269	112	1	however	however	ADV
ajlm-269	112	2	,	,	PUNCT
ajlm-269	112	3	hcv	hcv	X
ajlm-269	112	4	surveillance	surveillance	NOUN
ajlm-269	112	5	may	may	AUX
ajlm-269	112	6	still	still	ADV
ajlm-269	112	7	be	be	AUX
ajlm-269	112	8	prudent	prudent	ADJ
ajlm-269	112	9	for	for	ADP
ajlm-269	112	10	hiv	hiv	PROPN
ajlm-269	112	11	-	-	PUNCT
ajlm-269	112	12	exposed	expose	VERB
ajlm-269	112	13	infants	infant	NOUN
ajlm-269	112	14	in	in	ADP
ajlm-269	112	15	countries	country	NOUN
ajlm-269	112	16	with	with	ADP
ajlm-269	112	17	moderate	moderate	ADJ
ajlm-269	112	18	to	to	PART
ajlm-269	112	19	high	high	VERB
ajlm-269	112	20	hiv	hiv	PROPN
ajlm-269	112	21	and	and	CCONJ
ajlm-269	112	22	hcv	hcv	VERB
ajlm-269	112	23	prevalence	prevalence	NOUN
ajlm-269	112	24	and	and	CCONJ
ajlm-269	112	25	should	should	AUX
ajlm-269	112	26	include	include	VERB
ajlm-269	112	27	collection	collection	NOUN
ajlm-269	112	28	of	of	ADP
ajlm-269	112	29	clinical	clinical	ADJ
ajlm-269	112	30	information	information	NOUN
ajlm-269	112	31	and	and	CCONJ
ajlm-269	112	32	data	datum	NOUN
ajlm-269	112	33	about	about	ADP
ajlm-269	112	34	confounding	confound	VERB
ajlm-269	112	35	factors	factor	NOUN
ajlm-269	112	36	.	.	PUNCT
ajlm-269	113	1	acknowledgements	acknowledgement	NOUN
ajlm-269	113	2	competing	compete	VERB
ajlm-269	113	3	interests	interest	NOUN
ajlm-269	113	4	the	the	DET
ajlm-269	113	5	authors	author	NOUN
ajlm-269	113	6	declare	declare	VERB
ajlm-269	113	7	that	that	SCONJ
ajlm-269	113	8	they	they	PRON
ajlm-269	113	9	have	have	VERB
ajlm-269	113	10	no	no	DET
ajlm-269	113	11	financial	financial	ADJ
ajlm-269	113	12	or	or	CCONJ
ajlm-269	113	13	personal	personal	ADJ
ajlm-269	113	14	relationship(s	relationship(s	NOUN
ajlm-269	113	15	)	)	PUNCT
ajlm-269	113	16	which	which	PRON
ajlm-269	113	17	may	may	AUX
ajlm-269	113	18	have	have	AUX
ajlm-269	113	19	inappropriately	inappropriately	ADV
ajlm-269	113	20	influenced	influence	VERB
ajlm-269	113	21	them	they	PRON
ajlm-269	113	22	in	in	ADP
ajlm-269	113	23	writing	write	VERB
ajlm-269	113	24	this	this	DET
ajlm-269	113	25	article	article	NOUN
ajlm-269	113	26	.	.	PUNCT
ajlm-269	114	1	sources	source	NOUN
ajlm-269	114	2	of	of	ADP
ajlm-269	114	3	support	support	NOUN
ajlm-269	114	4	we	we	PRON
ajlm-269	114	5	would	would	AUX
ajlm-269	114	6	like	like	VERB
ajlm-269	114	7	to	to	PART
ajlm-269	114	8	thank	thank	VERB
ajlm-269	114	9	the	the	DET
ajlm-269	114	10	national	national	PROPN
ajlm-269	114	11	institute	institute	PROPN
ajlm-269	114	12	of	of	ADP
ajlm-269	114	13	communicable	communicable	NOUN
ajlm-269	114	14	diseases	disease	NOUN
ajlm-269	114	15	,	,	PUNCT
ajlm-269	114	16	gauteng	gauteng	NOUN
ajlm-269	114	17	for	for	ADP
ajlm-269	114	18	providing	provide	VERB
ajlm-269	114	19	us	we	PRON
ajlm-269	114	20	with	with	ADP
ajlm-269	114	21	serum	serum	NOUN
ajlm-269	114	22	samples	sample	NOUN
ajlm-269	114	23	positive	positive	ADJ
ajlm-269	114	24	for	for	ADP
ajlm-269	114	25	hcv	hcv	NOUN
ajlm-269	114	26	.	.	PUNCT
ajlm-269	115	1	we	we	PRON
ajlm-269	115	2	also	also	ADV
ajlm-269	115	3	appreciate	appreciate	VERB
ajlm-269	115	4	the	the	DET
ajlm-269	115	5	south	south	ADJ
ajlm-269	115	6	african	african	ADJ
ajlm-269	115	7	national	national	ADJ
ajlm-269	115	8	blood	blood	NOUN
ajlm-269	115	9	services	service	NOUN
ajlm-269	115	10	,	,	PUNCT
ajlm-269	115	11	durban	durban	NOUN
ajlm-269	115	12	providing	provide	VERB
ajlm-269	115	13	us	we	PRON
ajlm-269	115	14	with	with	ADP
ajlm-269	115	15	hcv	hcv	ADJ
ajlm-269	115	16	-	-	PUNCT
ajlm-269	115	17	negative	negative	ADJ
ajlm-269	115	18	blood	blood	NOUN
ajlm-269	115	19	.	.	PUNCT
ajlm-269	116	1	authors	author	NOUN
ajlm-269	116	2	’	'	PUNCT
ajlm-269	116	3	contributions	contribution	NOUN
ajlm-269	116	4	r.p	r.p	PROPN
ajlm-269	116	5	.	.	PUNCT
ajlm-269	116	6	(	(	PUNCT
ajlm-269	116	7	university	university	NOUN
ajlm-269	116	8	of	of	ADP
ajlm-269	116	9	kwazulu	kwazulu	PROPN
ajlm-269	116	10	-	-	PUNCT
ajlm-269	116	11	natal	natal	ADJ
ajlm-269	116	12	/	/	SYM
ajlm-269	116	13	national	national	PROPN
ajlm-269	116	14	health	health	PROPN
ajlm-269	116	15	laboratory	laboratory	PROPN
ajlm-269	116	16	services	service	NOUN
ajlm-269	116	17	)	)	PUNCT
ajlm-269	116	18	designed	design	VERB
ajlm-269	116	19	the	the	DET
ajlm-269	116	20	project	project	NOUN
ajlm-269	116	21	and	and	CCONJ
ajlm-269	116	22	analysed	analyse	VERB
ajlm-269	116	23	the	the	DET
ajlm-269	116	24	data	datum	NOUN
ajlm-269	116	25	.	.	PUNCT
ajlm-269	117	1	a.n	a.n	PROPN
ajlm-269	117	2	.	.	PROPN
ajlm-269	118	1	(	(	PUNCT
ajlm-269	118	2	university	university	NOUN
ajlm-269	118	3	of	of	ADP
ajlm-269	118	4	kwazulu	kwazulu	PROPN
ajlm-269	118	5	-	-	PUNCT
ajlm-269	118	6	natal	natal	ADJ
ajlm-269	118	7	/	/	SYM
ajlm-269	118	8	national	national	PROPN
ajlm-269	118	9	health	health	PROPN
ajlm-269	118	10	laboratory	laboratory	PROPN
ajlm-269	118	11	services	service	NOUN
ajlm-269	118	12	)	)	PUNCT
ajlm-269	118	13	performed	perform	VERB
ajlm-269	118	14	the	the	DET
ajlm-269	118	15	laboratory	laboratory	NOUN
ajlm-269	118	16	work	work	NOUN
ajlm-269	118	17	and	and	CCONJ
ajlm-269	118	18	was	be	AUX
ajlm-269	118	19	responsible	responsible	ADJ
ajlm-269	118	20	for	for	ADP
ajlm-269	118	21	writing	write	VERB
ajlm-269	118	22	the	the	DET
ajlm-269	118	23	manuscript	manuscript	NOUN
ajlm-269	118	24	.	.	PUNCT
ajlm-269	119	1	p.m.	p.m.	NOUN
ajlm-269	120	1	(	(	PUNCT
ajlm-269	120	2	university	university	NOUN
ajlm-269	120	3	of	of	ADP
ajlm-269	120	4	kwazulu	kwazulu	PROPN
ajlm-269	120	5	-	-	PUNCT
ajlm-269	120	6	natal	natal	ADJ
ajlm-269	120	7	/	/	SYM
ajlm-269	120	8	national	national	PROPN
ajlm-269	120	9	health	health	PROPN
ajlm-269	120	10	laboratory	laboratory	PROPN
ajlm-269	120	11	services	service	NOUN
ajlm-269	120	12	)	)	PUNCT
ajlm-269	120	13	assisted	assist	VERB
ajlm-269	120	14	in	in	ADP
ajlm-269	120	15	writing	writing	NOUN
ajlm-269	120	16	and	and	CCONJ
ajlm-269	120	17	preparing	prepare	VERB
ajlm-269	120	18	the	the	DET
ajlm-269	120	19	article	article	NOUN
ajlm-269	120	20	references	reference	VERB
ajlm-269	120	21	lavanchy	lavanchy	PROPN
ajlm-269	120	22	d.	d.	PROPN
ajlm-269	120	23	evolving	evolve	VERB
ajlm-269	120	24	epidemiology	epidemiology	NOUN
ajlm-269	120	25	of	of	ADP
ajlm-269	120	26	hepatitis	hepatitis	PROPN
ajlm-269	120	27	c	c	PROPN
ajlm-269	120	28	virus	virus	PROPN
ajlm-269	120	29	.	.	PUNCT
ajlm-269	121	1	clin	clin	PROPN
ajlm-269	121	2	microbiol	microbiol	PROPN
ajlm-269	121	3	infect	infect	PROPN
ajlm-269	121	4	.	.	PUNCT
ajlm-269	122	1	2011;17(2):107–115	2011;17(2):107–115	X
ajlm-269	122	2	.	.	PUNCT
ajlm-269	123	1	http://dx.doi.org/10.1111/j.1469-0691.2010.03432.x	http://dx.doi.org/10.1111/j.1469-0691.2010.03432.x	PROPN
ajlm-269	123	2	nel	nel	PROPN
ajlm-269	123	3	e	e	PROPN
ajlm-269	123	4	,	,	PUNCT
ajlm-269	123	5	sokol	sokol	PROPN
ajlm-269	123	6	rj	rj	PROPN
ajlm-269	123	7	,	,	PUNCT
ajlm-269	123	8	comparcola	comparcola	PROPN
ajlm-269	124	1	d	d	PROPN
ajlm-269	124	2	,	,	PUNCT
ajlm-269	124	3	et	et	PROPN
ajlm-269	124	4	al	al	PROPN
ajlm-269	124	5	.	.	PROPN
ajlm-269	124	6	viral	viral	ADJ
ajlm-269	124	7	hepatitis	hepatitis	NOUN
ajlm-269	124	8	in	in	ADP
ajlm-269	124	9	children	child	NOUN
ajlm-269	124	10	.	.	PUNCT
ajlm-269	125	1	j	j	PROPN
ajlm-269	125	2	paediatr	paediatr	PROPN
ajlm-269	125	3	gastroenterol	gastroenterol	PROPN
ajlm-269	125	4	nutr	nutr	PROPN
ajlm-269	125	5	.	.	PROPN
ajlm-269	125	6	2012;55(5):500–505	2012;55(5):500–505	NUM
ajlm-269	125	7	.	.	PUNCT
ajlm-269	126	1	http://dx.doi.org/10.1097/mpg.0b013e318272aee7	http://dx.doi.org/10.1097/mpg.0b013e318272aee7	PROPN
ajlm-269	126	2	yeung	yeung	PROPN
ajlm-269	126	3	c	c	PROPN
ajlm-269	126	4	-	-	PUNCT
ajlm-269	126	5	y	y	PROPN
ajlm-269	126	6	,	,	PUNCT
ajlm-269	126	7	lee	lee	PROPN
ajlm-269	126	8	h	h	PROPN
ajlm-269	126	9	-	-	PUNCT
ajlm-269	126	10	c	c	PROPN
ajlm-269	126	11	,	,	PUNCT
ajlm-269	126	12	chan	chan	PROPN
ajlm-269	126	13	w	w	PROPN
ajlm-269	126	14	-	-	PUNCT
ajlm-269	126	15	t	t	PROPN
ajlm-269	126	16	,	,	PUNCT
ajlm-269	126	17	et	et	PROPN
ajlm-269	126	18	al	al	PROPN
ajlm-269	126	19	.	.	PROPN
ajlm-269	126	20	vertical	vertical	ADJ
ajlm-269	126	21	transmission	transmission	NOUN
ajlm-269	126	22	of	of	ADP
ajlm-269	126	23	hepatitis	hepatitis	PROPN
ajlm-269	126	24	c	c	PROPN
ajlm-269	126	25	virus	virus	NOUN
ajlm-269	126	26	:	:	PUNCT
ajlm-269	126	27	current	current	ADJ
ajlm-269	126	28	knowledge	knowledge	NOUN
ajlm-269	126	29	and	and	CCONJ
ajlm-269	126	30	perspectives	perspective	NOUN
ajlm-269	126	31	.	.	PUNCT
ajlm-269	127	1	world	world	NOUN
ajlm-269	127	2	j	j	PROPN
ajlm-269	127	3	hepatol	hepatol	ADJ
ajlm-269	127	4	.	.	PUNCT
ajlm-269	128	1	2014;6(9):643–651	2014;6(9):643–651	X
ajlm-269	128	2	.	.	NUM
ajlm-269	128	3	http://dx.doi.org/10.4254/wjh.v6.i9.643	http://dx.doi.org/10.4254/wjh.v6.i9.643	PROPN
ajlm-269	128	4	operskalski	operskalski	PROPN
ajlm-269	128	5	ea	ea	PROPN
ajlm-269	128	6	,	,	PUNCT
ajlm-269	128	7	kovacs	kovacs	PROPN
ajlm-269	128	8	a.	a.	PROPN
ajlm-269	128	9	hiv	hiv	PROPN
ajlm-269	128	10	/	/	SYM
ajlm-269	128	11	hcv	hcv	X
ajlm-269	128	12	co	co	NOUN
ajlm-269	128	13	-	-	NOUN
ajlm-269	128	14	infection	infection	NOUN
ajlm-269	128	15	:	:	PUNCT
ajlm-269	129	1	pathogenesis	pathogenesis	NOUN
ajlm-269	129	2	,	,	PUNCT
ajlm-269	129	3	clinical	clinical	ADJ
ajlm-269	129	4	complications	complication	NOUN
ajlm-269	129	5	,	,	PUNCT
ajlm-269	129	6	treatment	treatment	NOUN
ajlm-269	129	7	,	,	PUNCT
ajlm-269	129	8	and	and	CCONJ
ajlm-269	129	9	new	new	ADJ
ajlm-269	129	10	therapeutic	therapeutic	ADJ
ajlm-269	129	11	technologies	technology	NOUN
ajlm-269	129	12	.	.	PUNCT
ajlm-269	130	1	curr	curr	PROPN
ajlm-269	130	2	hiv	hiv	PROPN
ajlm-269	130	3	/	/	SYM
ajlm-269	130	4	aids	aids	PROPN
ajlm-269	130	5	rep	rep	PROPN
ajlm-269	130	6	.	.	PROPN
ajlm-269	130	7	2011;8(1):12–22	2011;8(1):12–22	NUM
ajlm-269	130	8	.	.	PUNCT
ajlm-269	130	9	http://dx.doi.org/10.1007/s11904-010-0071-3	http://dx.doi.org/10.1007/s11904-010-0071-3	NUM
ajlm-269	130	10	barth	barth	PROPN
ajlm-269	130	11	re	re	PROPN
ajlm-269	130	12	,	,	PUNCT
ajlm-269	130	13	huijgen	huijgen	PROPN
ajlm-269	130	14	q	q	X
ajlm-269	130	15	,	,	PUNCT
ajlm-269	130	16	taljaard	taljaard	PROPN
ajlm-269	130	17	j	j	PROPN
ajlm-269	130	18	,	,	PUNCT
ajlm-269	130	19	et	et	PROPN
ajlm-269	130	20	al	al	PROPN
ajlm-269	130	21	.	.	PUNCT
ajlm-269	131	1	hepatitis	hepatitis	PROPN
ajlm-269	131	2	b	b	PROPN
ajlm-269	131	3	/	/	SYM
ajlm-269	131	4	c	c	PROPN
ajlm-269	131	5	and	and	CCONJ
ajlm-269	131	6	hiv	hiv	PROPN
ajlm-269	131	7	in	in	ADP
ajlm-269	131	8	sub	sub	ADJ
ajlm-269	131	9	-	-	ADJ
ajlm-269	131	10	saharan	saharan	ADJ
ajlm-269	131	11	africa	africa	PROPN
ajlm-269	131	12	:	:	PUNCT
ajlm-269	131	13	an	an	DET
ajlm-269	131	14	association	association	NOUN
ajlm-269	131	15	between	between	ADP
ajlm-269	131	16	highly	highly	ADV
ajlm-269	131	17	prevalent	prevalent	ADJ
ajlm-269	131	18	infectious	infectious	ADJ
ajlm-269	131	19	diseases	disease	NOUN
ajlm-269	131	20	.	.	PUNCT
ajlm-269	132	1	a	a	DET
ajlm-269	132	2	systematic	systematic	ADJ
ajlm-269	132	3	review	review	NOUN
ajlm-269	132	4	and	and	CCONJ
ajlm-269	132	5	meta	meta	ADJ
ajlm-269	132	6	-	-	PUNCT
ajlm-269	132	7	analysis	analysis	NOUN
ajlm-269	132	8	.	.	PUNCT
ajlm-269	133	1	int	int	PROPN
ajlm-269	133	2	j	j	PROPN
ajlm-269	133	3	infect	infect	VERB
ajlm-269	133	4	dis	dis	PROPN
ajlm-269	133	5	.	.	PUNCT
ajlm-269	134	1	2010;14(12):e1024	2010;14(12):e1024	NOUN
ajlm-269	134	2	–	–	PUNCT
ajlm-269	134	3	e1031	e1031	NOUN
ajlm-269	134	4	.	.	PUNCT
ajlm-269	135	1	http://dx.doi.org/10.1016/j.ijid.2010.06.013	http://dx.doi.org/10.1016/j.ijid.2010.06.013	PROPN
ajlm-269	135	2	hoffmann	hoffmann	PROPN
ajlm-269	135	3	cj	cj	PROPN
ajlm-269	135	4	,	,	PUNCT
ajlm-269	135	5	dayal	dayal	PROPN
ajlm-269	135	6	d	d	PROPN
ajlm-269	135	7	,	,	PUNCT
ajlm-269	135	8	cheyip	cheyip	PROPN
ajlm-269	135	9	m	m	PROPN
ajlm-269	135	10	,	,	PUNCT
ajlm-269	135	11	et	et	PROPN
ajlm-269	135	12	al	al	PROPN
ajlm-269	135	13	.	.	PUNCT
ajlm-269	135	14	prevalence	prevalence	NOUN
ajlm-269	135	15	and	and	CCONJ
ajlm-269	135	16	associations	association	NOUN
ajlm-269	135	17	with	with	ADP
ajlm-269	135	18	hepatitis	hepatitis	PROPN
ajlm-269	135	19	b	b	PROPN
ajlm-269	135	20	and	and	CCONJ
ajlm-269	135	21	hepatitis	hepatitis	PROPN
ajlm-269	135	22	c	c	NOUN
ajlm-269	135	23	infection	infection	NOUN
ajlm-269	135	24	among	among	ADP
ajlm-269	135	25	hiv	hiv	PROPN
ajlm-269	135	26	-	-	PUNCT
ajlm-269	135	27	infected	infect	VERB
ajlm-269	135	28	adults	adult	NOUN
ajlm-269	135	29	in	in	ADP
ajlm-269	135	30	south	south	PROPN
ajlm-269	135	31	africa	africa	PROPN
ajlm-269	135	32	.	.	PUNCT
ajlm-269	136	1	int	int	PROPN
ajlm-269	136	2	j	j	PROPN
ajlm-269	136	3	std	std	X
ajlm-269	136	4	aids	aids	PROPN
ajlm-269	136	5	.	.	PUNCT
ajlm-269	137	1	2012;23(10):e10	2012;23(10):e10	NUM
ajlm-269	137	2	–	–	PUNCT
ajlm-269	137	3	e13	e13	PROPN
ajlm-269	137	4	.	.	PUNCT
ajlm-269	138	1	http://dx.doi.org/10.1258/ijsa.2009.009340	http://dx.doi.org/10.1258/ijsa.2009.009340	VERB
ajlm-269	138	2	madhava	madhava	PROPN
ajlm-269	138	3	v	v	PROPN
ajlm-269	138	4	,	,	PUNCT
ajlm-269	138	5	burgess	burgess	PROPN
ajlm-269	138	6	c	c	PROPN
ajlm-269	138	7	,	,	PUNCT
ajlm-269	138	8	drucker	drucker	PROPN
ajlm-269	138	9	e.	e.	PROPN
ajlm-269	138	10	epidemiology	epidemiology	PROPN
ajlm-269	138	11	of	of	ADP
ajlm-269	138	12	chronic	chronic	ADJ
ajlm-269	138	13	hepatitis	hepatitis	PROPN
ajlm-269	138	14	c	c	PROPN
ajlm-269	138	15	virus	virus	NOUN
ajlm-269	138	16	infection	infection	NOUN
ajlm-269	138	17	in	in	ADP
ajlm-269	138	18	sub	sub	ADJ
ajlm-269	138	19	-	-	ADJ
ajlm-269	138	20	saharan	saharan	ADJ
ajlm-269	138	21	africa	africa	PROPN
ajlm-269	138	22	.	.	PUNCT
ajlm-269	139	1	lancet	lancet	PROPN
ajlm-269	139	2	infect	infect	PROPN
ajlm-269	139	3	dis	dis	PROPN
ajlm-269	139	4	.	.	PUNCT
ajlm-269	140	1	2002;2(5):293–302	2002;2(5):293–302	X
ajlm-269	140	2	.	.	X
ajlm-269	140	3	http://dx.doi.org/10.1016/s1473-3099(02)00264-5	http://dx.doi.org/10.1016/s1473-3099(02)00264-5	PROPN
ajlm-269	140	4	national	national	PROPN
ajlm-269	140	5	department	department	PROPN
ajlm-269	140	6	of	of	ADP
ajlm-269	140	7	health	health	PROPN
ajlm-269	140	8	.	.	PUNCT
ajlm-269	141	1	the	the	DET
ajlm-269	141	2	2013	2013	NUM
ajlm-269	141	3	national	national	ADJ
ajlm-269	141	4	antenatal	antenatal	ADJ
ajlm-269	141	5	sentinel	sentinel	NOUN
ajlm-269	141	6	hiv	hiv	PROPN
ajlm-269	141	7	prevalence	prevalence	NOUN
ajlm-269	141	8	survey	survey	NOUN
ajlm-269	141	9	,	,	PUNCT
ajlm-269	141	10	south	south	PROPN
ajlm-269	141	11	africa	africa	PROPN
ajlm-269	141	12	.	.	PUNCT
ajlm-269	142	1	pretoria	pretoria	PROPN
ajlm-269	142	2	:	:	PUNCT
ajlm-269	142	3	national	national	PROPN
ajlm-269	142	4	department	department	PROPN
ajlm-269	142	5	of	of	ADP
ajlm-269	142	6	health	health	NOUN
ajlm-269	142	7	;	;	PUNCT
ajlm-269	142	8	2013	2013	NUM
ajlm-269	142	9	.	.	PUNCT
ajlm-269	143	1	parboosing	parboose	VERB
ajlm-269	143	2	r	r	NOUN
ajlm-269	143	3	,	,	PUNCT
ajlm-269	143	4	paruk	paruk	NOUN
ajlm-269	143	5	i	i	PRON
ajlm-269	143	6	,	,	PUNCT
ajlm-269	143	7	lalloo	lalloo	NOUN
ajlm-269	143	8	ug	ug	ADP
ajlm-269	143	9	.	.	PUNCT
ajlm-269	144	1	hepatitis	hepatitis	PROPN
ajlm-269	144	2	c	c	PROPN
ajlm-269	144	3	virus	virus	NOUN
ajlm-269	144	4	seropositivity	seropositivity	NOUN
ajlm-269	144	5	in	in	ADP
ajlm-269	144	6	a	a	DET
ajlm-269	144	7	south	south	ADJ
ajlm-269	144	8	african	african	ADJ
ajlm-269	144	9	cohort	cohort	NOUN
ajlm-269	144	10	of	of	ADP
ajlm-269	144	11	hiv	hiv	PROPN
ajlm-269	144	12	co	co	PROPN
ajlm-269	144	13	-	-	VERB
ajlm-269	144	14	infected	infected	ADJ
ajlm-269	144	15	,	,	PUNCT
ajlm-269	144	16	arv	arv	NOUN
ajlm-269	144	17	naïve	naïve	ADJ
ajlm-269	144	18	patients	patient	NOUN
ajlm-269	144	19	is	be	AUX
ajlm-269	144	20	associated	associate	VERB
ajlm-269	144	21	with	with	ADP
ajlm-269	144	22	renal	renal	ADJ
ajlm-269	144	23	insufficiency	insufficiency	NOUN
ajlm-269	144	24	and	and	CCONJ
ajlm-269	144	25	increased	increase	VERB
ajlm-269	144	26	mortality	mortality	NOUN
ajlm-269	144	27	.	.	PUNCT
ajlm-269	145	1	j	j	PROPN
ajlm-269	145	2	med	med	PROPN
ajlm-269	145	3	virol	virol	NOUN
ajlm-269	145	4	.	.	PUNCT
ajlm-269	146	1	2008;80(9):1530–1536	2008;80(9):1530–1536	PROPN
ajlm-269	146	2	.	.	PUNCT
ajlm-269	147	1	http://dx.doi.org/10.1002/jmv.21262	http://dx.doi.org/10.1002/jmv.21262	PROPN
ajlm-269	147	2	world	world	PROPN
ajlm-269	147	3	health	health	PROPN
ajlm-269	147	4	organization	organization	NOUN
ajlm-269	147	5	.	.	PUNCT
ajlm-269	148	1	guidelines	guideline	NOUN
ajlm-269	148	2	for	for	ADP
ajlm-269	148	3	the	the	DET
ajlm-269	148	4	screening	screening	NOUN
ajlm-269	148	5	,	,	PUNCT
ajlm-269	148	6	care	care	NOUN
ajlm-269	148	7	and	and	CCONJ
ajlm-269	148	8	treatment	treatment	NOUN
ajlm-269	148	9	of	of	ADP
ajlm-269	148	10	persons	person	NOUN
ajlm-269	148	11	with	with	ADP
ajlm-269	148	12	hepatitis	hepatitis	PROPN
ajlm-269	148	13	c	c	NOUN
ajlm-269	148	14	infection	infection	NOUN
ajlm-269	148	15	.	.	PUNCT
ajlm-269	149	1	geneva	geneva	PROPN
ajlm-269	149	2	:	:	PUNCT
ajlm-269	149	3	world	world	PROPN
ajlm-269	149	4	health	health	NOUN
ajlm-269	149	5	organization	organization	NOUN
ajlm-269	149	6	;	;	PUNCT
ajlm-269	149	7	2014	2014	NUM
ajlm-269	149	8	.	.	PUNCT
ajlm-269	150	1	smit	smit	PROPN
ajlm-269	150	2	pw	pw	PROPN
ajlm-269	150	3	,	,	PUNCT
ajlm-269	150	4	elliott	elliott	PROPN
ajlm-269	150	5	i	i	PROPN
ajlm-269	150	6	,	,	PUNCT
ajlm-269	150	7	peeling	peel	VERB
ajlm-269	150	8	rw	rw	NOUN
ajlm-269	150	9	,	,	PUNCT
ajlm-269	150	10	et	et	PROPN
ajlm-269	150	11	al	al	PROPN
ajlm-269	150	12	.	.	PUNCT
ajlm-269	151	1	an	an	DET
ajlm-269	151	2	overview	overview	NOUN
ajlm-269	151	3	of	of	ADP
ajlm-269	151	4	the	the	DET
ajlm-269	151	5	clinical	clinical	ADJ
ajlm-269	151	6	use	use	NOUN
ajlm-269	151	7	of	of	ADP
ajlm-269	151	8	filter	filter	NOUN
ajlm-269	151	9	paper	paper	NOUN
ajlm-269	151	10	in	in	ADP
ajlm-269	151	11	the	the	DET
ajlm-269	151	12	diagnosis	diagnosis	NOUN
ajlm-269	151	13	of	of	ADP
ajlm-269	151	14	tropical	tropical	ADJ
ajlm-269	151	15	diseases	disease	NOUN
ajlm-269	151	16	.	.	PUNCT
ajlm-269	152	1	am	be	AUX
ajlm-269	152	2	j	j	PROPN
ajlm-269	152	3	trop	trop	X
ajlm-269	152	4	med	med	PROPN
ajlm-269	152	5	hyg	hyg	PROPN
ajlm-269	152	6	.	.	PUNCT
ajlm-269	153	1	2014;90(2):195–210	2014;90(2):195–210	PROPN
ajlm-269	153	2	.	.	PUNCT
ajlm-269	154	1	http://dx.doi.org/10.4269/ajtmh.13-0463	http://dx.doi.org/10.4269/ajtmh.13-0463	ADJ
ajlm-269	154	2	albertoni	albertoni	NOUN
ajlm-269	154	3	g	g	PROPN
ajlm-269	154	4	,	,	PUNCT
ajlm-269	154	5	castelo	castelo	NOUN
ajlm-269	154	6	girão	girão	PROPN
ajlm-269	154	7	m	m	PROPN
ajlm-269	154	8	,	,	PUNCT
ajlm-269	154	9	schor	schor	PROPN
ajlm-269	154	10	n.	n.	PROPN
ajlm-269	154	11	mini	mini	PROPN
ajlm-269	154	12	review	review	PROPN
ajlm-269	154	13	:	:	PUNCT
ajlm-269	154	14	current	current	ADJ
ajlm-269	154	15	molecular	molecular	ADJ
ajlm-269	154	16	methods	method	NOUN
ajlm-269	154	17	for	for	ADP
ajlm-269	154	18	the	the	DET
ajlm-269	154	19	detection	detection	NOUN
ajlm-269	154	20	and	and	CCONJ
ajlm-269	154	21	quantification	quantification	NOUN
ajlm-269	154	22	of	of	ADP
ajlm-269	154	23	hepatitis	hepatitis	PROPN
ajlm-269	154	24	b	b	PROPN
ajlm-269	154	25	virus	virus	NOUN
ajlm-269	154	26	,	,	PUNCT
ajlm-269	154	27	hepatitis	hepatitis	PROPN
ajlm-269	154	28	c	c	PROPN
ajlm-269	154	29	virus	virus	NOUN
ajlm-269	154	30	,	,	PUNCT
ajlm-269	154	31	and	and	CCONJ
ajlm-269	154	32	human	human	ADJ
ajlm-269	154	33	immunodeficiency	immunodeficiency	NOUN
ajlm-269	154	34	virus	virus	NOUN
ajlm-269	154	35	type	type	NOUN
ajlm-269	154	36	1	1	NUM
ajlm-269	154	37	.	.	PUNCT
ajlm-269	154	38	int	int	PROPN
ajlm-269	154	39	j	j	PROPN
ajlm-269	154	40	infect	infect	VERB
ajlm-269	154	41	dis	dis	PROPN
ajlm-269	154	42	.	.	PUNCT
ajlm-269	155	1	2014;25:145–149	2014;25:145–149	X
ajlm-269	155	2	.	.	PUNCT
ajlm-269	156	1	http://dx.doi.org/10.1016/j.ijid.2014.04.007	http://dx.doi.org/10.1016/j.ijid.2014.04.007	PROPN
ajlm-269	156	2	chevaliez	chevaliez	PROPN
ajlm-269	156	3	s	s	PROPN
ajlm-269	156	4	,	,	PUNCT
ajlm-269	156	5	bouvier	bouvier	NOUN
ajlm-269	156	6	-	-	PUNCT
ajlm-269	156	7	alias	alias	PROPN
ajlm-269	156	8	m	m	PROPN
ajlm-269	156	9	,	,	PUNCT
ajlm-269	156	10	rodriguez	rodriguez	PROPN
ajlm-269	156	11	c	c	PROPN
ajlm-269	156	12	,	,	PUNCT
ajlm-269	156	13	et	et	PROPN
ajlm-269	156	14	al	al	PROPN
ajlm-269	156	15	.	.	PUNCT
ajlm-269	157	1	the	the	DET
ajlm-269	157	2	cobas	cobas	PROPN
ajlm-269	157	3	ampliprep	ampliprep	PROPN
ajlm-269	157	4	/	/	SYM
ajlm-269	157	5	cobas	cobas	PROPN
ajlm-269	157	6	taqman	taqman	NOUN
ajlm-269	157	7	hcv	hcv	PROPN
ajlm-269	157	8	test	test	NOUN
ajlm-269	157	9	,	,	PUNCT
ajlm-269	157	10	version	version	NOUN
ajlm-269	157	11	2.0	2.0	NUM
ajlm-269	157	12	,	,	PUNCT
ajlm-269	157	13	real	real	ADJ
ajlm-269	157	14	-	-	PUNCT
ajlm-269	157	15	time	time	NOUN
ajlm-269	157	16	pcr	pcr	PROPN
ajlm-269	157	17	assay	assay	VERB
ajlm-269	157	18	accurately	accurately	ADV
ajlm-269	157	19	quantifies	quantify	VERB
ajlm-269	157	20	hepatitis	hepatitis	PROPN
ajlm-269	157	21	c	c	PROPN
ajlm-269	157	22	virus	virus	PROPN
ajlm-269	157	23	genotype	genotype	NOUN
ajlm-269	157	24	4	4	NUM
ajlm-269	157	25	rna	rna	NOUN
ajlm-269	157	26	.	.	PUNCT
ajlm-269	158	1	j	j	PROPN
ajlm-269	158	2	clin	clin	PROPN
ajlm-269	158	3	microbiol	microbiol	PROPN
ajlm-269	158	4	.	.	PUNCT
ajlm-269	159	1	2013;51(4):1078–1082	2013;51(4):1078–1082	NUM
ajlm-269	159	2	.	.	PUNCT
ajlm-269	159	3	http://dx.doi.org/10.1128/jcm.02004-12	http://dx.doi.org/10.1128/jcm.02004-12	VERB
ajlm-269	159	4	laughlin	laughlin	PROPN
ajlm-269	159	5	ts	ts	PROPN
ajlm-269	159	6	,	,	PUNCT
ajlm-269	159	7	nuccie	nuccie	NOUN
ajlm-269	159	8	b	b	PROPN
ajlm-269	159	9	,	,	PUNCT
ajlm-269	159	10	rothberg	rothberg	PROPN
ajlm-269	159	11	pg	pg	PROPN
ajlm-269	159	12	.	.	PUNCT
ajlm-269	160	1	genotyping	genotype	VERB
ajlm-269	160	2	of	of	ADP
ajlm-269	160	3	hepatitis	hepatitis	PROPN
ajlm-269	160	4	c	c	PROPN
ajlm-269	160	5	virus	virus	NOUN
ajlm-269	160	6	by	by	ADP
ajlm-269	160	7	sequence	sequence	NOUN
ajlm-269	160	8	analysis	analysis	NOUN
ajlm-269	160	9	of	of	ADP
ajlm-269	160	10	the	the	DET
ajlm-269	160	11	amplicon	amplicon	NOUN
ajlm-269	160	12	from	from	ADP
ajlm-269	160	13	the	the	DET
ajlm-269	160	14	roche	roche	PROPN
ajlm-269	160	15	cobas	cobas	PROPN
ajlm-269	160	16	ampliprep	ampliprep	PROPN
ajlm-269	160	17	/	/	SYM
ajlm-269	160	18	cobas	cobas	ADJ
ajlm-269	160	19	taqman	taqman	NOUN
ajlm-269	160	20	viral	viral	ADJ
ajlm-269	160	21	load	load	NOUN
ajlm-269	160	22	assay	assay	VERB
ajlm-269	160	23	.	.	PUNCT
ajlm-269	161	1	j	j	PROPN
ajlm-269	161	2	clin	clin	PROPN
ajlm-269	161	3	microbiol	microbiol	PROPN
ajlm-269	161	4	.	.	PUNCT
ajlm-269	162	1	2010;48(2):671–672	2010;48(2):671–672	X
ajlm-269	162	2	.	.	PROPN
ajlm-269	162	3	http://dx.doi.org/10.1128/jcm.01519-09	http://dx.doi.org/10.1128/jcm.01519-09	VERB
ajlm-269	162	4	mousavi	mousavi	PROPN
ajlm-269	162	5	-	-	PUNCT
ajlm-269	162	6	fard	fard	NOUN
ajlm-269	162	7	sh	sh	PROPN
ajlm-269	162	8	,	,	PUNCT
ajlm-269	162	9	merat	merat	PROPN
ajlm-269	162	10	s	s	PART
ajlm-269	162	11	,	,	PUNCT
ajlm-269	162	12	shahzamani	shahzamani	X
ajlm-269	162	13	k	k	NOUN
ajlm-269	162	14	,	,	PUNCT
ajlm-269	162	15	et	et	PROPN
ajlm-269	162	16	al	al	PROPN
ajlm-269	162	17	.	.	PUNCT
ajlm-269	163	1	development	development	NOUN
ajlm-269	163	2	of	of	ADP
ajlm-269	163	3	a	a	DET
ajlm-269	163	4	sybr	sybr	ADJ
ajlm-269	163	5	green	green	ADJ
ajlm-269	163	6	real	real	ADJ
ajlm-269	163	7	time	time	NOUN
ajlm-269	163	8	multiplex	multiplex	PROPN
ajlm-269	163	9	rt	rt	PROPN
ajlm-269	163	10	-	-	PUNCT
ajlm-269	163	11	pcr	pcr	NOUN
ajlm-269	163	12	technique	technique	NOUN
ajlm-269	163	13	for	for	ADP
ajlm-269	163	14	simultaneous	simultaneous	ADJ
ajlm-269	163	15	detection	detection	NOUN
ajlm-269	163	16	of	of	ADP
ajlm-269	163	17	hcv	hcv	NOUN
ajlm-269	163	18	and	and	CCONJ
ajlm-269	163	19	gbv	gbv	PROPN
ajlm-269	163	20	-	-	PUNCT
ajlm-269	163	21	c	c	NOUN
ajlm-269	163	22	co	co	NOUN
ajlm-269	163	23	-	-	NOUN
ajlm-269	163	24	infection	infection	NOUN
ajlm-269	163	25	in	in	ADP
ajlm-269	163	26	plasma	plasma	NOUN
ajlm-269	163	27	samples	sample	NOUN
ajlm-269	163	28	.	.	PUNCT
ajlm-269	164	1	mbd	mbd	NUM
ajlm-269	164	2	.	.	PUNCT
ajlm-269	165	1	2014;1(1):41	2014;1(1):41	NUM
ajlm-269	165	2	-	-	SYM
ajlm-269	165	3	49	49	NUM
ajlm-269	165	4	.	.	PUNCT
ajlm-269	166	1	paryan	paryan	PROPN
ajlm-269	166	2	m	m	PROPN
ajlm-269	166	3	,	,	PUNCT
ajlm-269	166	4	forouzandeh	forouzandeh	PROPN
ajlm-269	166	5	mm	mm	PROPN
ajlm-269	166	6	,	,	PUNCT
ajlm-269	166	7	kia	kia	PROPN
ajlm-269	166	8	v	v	PROPN
ajlm-269	166	9	,	,	PUNCT
ajlm-269	166	10	et	et	PROPN
ajlm-269	166	11	al	al	PROPN
ajlm-269	166	12	.	.	PROPN
ajlm-269	166	13	design	design	NOUN
ajlm-269	166	14	and	and	CCONJ
ajlm-269	166	15	development	development	NOUN
ajlm-269	166	16	of	of	ADP
ajlm-269	166	17	an	an	DET
ajlm-269	166	18	in	in	ADP
ajlm-269	166	19	-	-	PUNCT
ajlm-269	166	20	house	house	NOUN
ajlm-269	166	21	multiplex	multiplex	PROPN
ajlm-269	166	22	rt	rt	PROPN
ajlm-269	166	23	-	-	PUNCT
ajlm-269	166	24	pcr	pcr	PROPN
ajlm-269	166	25	assay	assay	NOUN
ajlm-269	166	26	for	for	ADP
ajlm-269	166	27	simultaneous	simultaneous	ADJ
ajlm-269	166	28	detection	detection	NOUN
ajlm-269	166	29	of	of	ADP
ajlm-269	166	30	hiv-1	hiv-1	PUNCT
ajlm-269	166	31	and	and	CCONJ
ajlm-269	166	32	hcv	hcv	NOUN
ajlm-269	166	33	in	in	ADP
ajlm-269	166	34	plasma	plasma	NOUN
ajlm-269	166	35	samples	sample	NOUN
ajlm-269	166	36	.	.	PUNCT
ajlm-269	167	1	iran	iran	PROPN
ajlm-269	167	2	j	j	PROPN
ajlm-269	167	3	microbiol	microbiol	PROPN
ajlm-269	167	4	.	.	PUNCT
ajlm-269	168	1	2012;4(1):8–14	2012;4(1):8–14	X
ajlm-269	168	2	.	.	PUNCT
ajlm-269	169	1	rozales	rozale	NOUN
ajlm-269	169	2	fd	fd	VERB
ajlm-269	169	3	,	,	PUNCT
ajlm-269	169	4	de	de	PROPN
ajlm-269	169	5	-	-	NOUN
ajlm-269	169	6	paris	paris	ADJ
ajlm-269	169	7	f	f	PROPN
ajlm-269	169	8	,	,	PUNCT
ajlm-269	169	9	machado	machado	PROPN
ajlm-269	169	10	abmp	abmp	PROPN
ajlm-269	169	11	,	,	PUNCT
ajlm-269	169	12	et	et	PROPN
ajlm-269	169	13	al	al	PROPN
ajlm-269	169	14	.	.	PUNCT
ajlm-269	169	15	optimization	optimization	NOUN
ajlm-269	169	16	of	of	ADP
ajlm-269	169	17	one	one	NUM
ajlm-269	169	18	-	-	PUNCT
ajlm-269	169	19	step	step	NOUN
ajlm-269	169	20	real	real	ADJ
ajlm-269	169	21	-	-	PUNCT
ajlm-269	169	22	time	time	NOUN
ajlm-269	169	23	pcr	pcr	NOUN
ajlm-269	169	24	for	for	ADP
ajlm-269	169	25	the	the	DET
ajlm-269	169	26	x	x	ADJ
ajlm-269	169	27	-	-	NOUN
ajlm-269	169	28	tail	tail	ADJ
ajlm-269	169	29	target	target	NOUN
ajlm-269	169	30	of	of	ADP
ajlm-269	169	31	hcv	hcv	PROPN
ajlm-269	169	32	as	as	ADP
ajlm-269	169	33	a	a	DET
ajlm-269	169	34	diagnostic	diagnostic	ADJ
ajlm-269	169	35	test	test	NOUN
ajlm-269	169	36	.	.	PUNCT
ajlm-269	170	1	clin	clin	PROPN
ajlm-269	170	2	biomed	biome	VERB
ajlm-269	170	3	res	re	NOUN
ajlm-269	170	4	.	.	PUNCT
ajlm-269	170	5	2014	2014	NUM
ajlm-269	170	6	;	;	PUNCT
ajlm-269	170	7	34(2):164–168	34(2):164–168	NUM
ajlm-269	170	8	.	.	PUNCT
ajlm-269	171	1	shahzamani	shahzamani	PROPN
ajlm-269	171	2	k	k	PROPN
ajlm-269	171	3	,	,	PUNCT
ajlm-269	171	4	sabahi	sabahi	PROPN
ajlm-269	172	1	f	f	X
ajlm-269	172	2	,	,	PUNCT
ajlm-269	172	3	merat	merat	PROPN
ajlm-269	172	4	s	s	PART
ajlm-269	172	5	,	,	PUNCT
ajlm-269	172	6	et	et	PROPN
ajlm-269	172	7	al	al	PROPN
ajlm-269	172	8	.	.	PROPN
ajlm-269	173	1	rapid	rapid	ADJ
ajlm-269	173	2	low	low	ADJ
ajlm-269	173	3	-	-	PUNCT
ajlm-269	173	4	cost	cost	NOUN
ajlm-269	173	5	detection	detection	NOUN
ajlm-269	173	6	of	of	ADP
ajlm-269	173	7	hepatitis	hepatitis	PROPN
ajlm-269	173	8	c	c	PROPN
ajlm-269	173	9	virus	virus	PROPN
ajlm-269	173	10	rna	rna	PROPN
ajlm-269	173	11	in	in	ADP
ajlm-269	173	12	hcv	hcv	NOUN
ajlm-269	173	13	-	-	PUNCT
ajlm-269	173	14	infected	infect	VERB
ajlm-269	173	15	patients	patient	NOUN
ajlm-269	173	16	by	by	ADP
ajlm-269	173	17	real	real	ADJ
ajlm-269	173	18	-	-	PUNCT
ajlm-269	173	19	time	time	NOUN
ajlm-269	173	20	rt	rt	NOUN
ajlm-269	173	21	-	-	PUNCT
ajlm-269	173	22	pcr	pcr	NOUN
ajlm-269	173	23	using	use	VERB
ajlm-269	173	24	sybr	sybr	PROPN
ajlm-269	173	25	green	green	PROPN
ajlm-269	173	26	i.	i.	PROPN
ajlm-269	173	27	arch	arch	PROPN
ajlm-269	173	28	iran	iran	PROPN
ajlm-269	173	29	med	me	VERB
ajlm-269	173	30	.	.	PUNCT
ajlm-269	174	1	2011;14(6):396–400	2011;14(6):396–400	X
ajlm-269	174	2	.	.	PUNCT
ajlm-269	175	1	zhang	zhang	PROPN
ajlm-269	175	2	ez	ez	PROPN
ajlm-269	175	3	,	,	PUNCT
ajlm-269	175	4	bartels	bartels	PROPN
ajlm-269	175	5	dj	dj	PROPN
ajlm-269	175	6	,	,	PUNCT
ajlm-269	175	7	frantz	frantz	PROPN
ajlm-269	175	8	jd	jd	PROPN
ajlm-269	175	9	,	,	PUNCT
ajlm-269	175	10	et	et	PROPN
ajlm-269	175	11	al	al	PROPN
ajlm-269	175	12	.	.	PUNCT
ajlm-269	175	13	development	development	NOUN
ajlm-269	175	14	of	of	ADP
ajlm-269	175	15	a	a	DET
ajlm-269	175	16	sensitive	sensitive	ADJ
ajlm-269	175	17	rt	rt	ADJ
ajlm-269	175	18	-	-	PUNCT
ajlm-269	175	19	pcr	pcr	ADJ
ajlm-269	175	20	method	method	NOUN
ajlm-269	175	21	for	for	ADP
ajlm-269	175	22	amplifying	amplify	VERB
ajlm-269	175	23	and	and	CCONJ
ajlm-269	175	24	sequencing	sequence	VERB
ajlm-269	175	25	near	near	ADP
ajlm-269	175	26	full	full	ADJ
ajlm-269	175	27	-	-	PUNCT
ajlm-269	175	28	length	length	NOUN
ajlm-269	175	29	hcv	hcv	NOUN
ajlm-269	175	30	genotype	genotype	NOUN
ajlm-269	175	31	1	1	NUM
ajlm-269	175	32	rna	rna	NOUN
ajlm-269	175	33	from	from	ADP
ajlm-269	175	34	patient	patient	ADJ
ajlm-269	175	35	samples	sample	NOUN
ajlm-269	175	36	.	.	PUNCT
ajlm-269	176	1	virol	virol	PROPN
ajlm-269	176	2	j.	j.	PROPN
ajlm-269	176	3	2013:10:53	2013:10:53	PROPN
ajlm-269	176	4	.	.	PUNCT
ajlm-269	177	1	http://dx.doi.org/10.1186/1743-422x-10-53	http://dx.doi.org/10.1186/1743-422x-10-53	PROPN
ajlm-269	177	2	zaghloul	zaghloul	PROPN
ajlm-269	177	3	mhe	mhe	PROPN
ajlm-269	177	4	,	,	PUNCT
ajlm-269	177	5	rizk	rizk	VERB
ajlm-269	177	6	em	em	PRON
ajlm-269	177	7	,	,	PUNCT
ajlm-269	177	8	elsayed	elsaye	VERB
ajlm-269	177	9	ar	ar	PROPN
ajlm-269	177	10	,	,	PUNCT
ajlm-269	178	1	et	et	PROPN
ajlm-269	178	2	al	al	PROPN
ajlm-269	178	3	.	.	PUNCT
ajlm-269	178	4	detection	detection	NOUN
ajlm-269	178	5	of	of	ADP
ajlm-269	178	6	hcv	hcv	NOUN
ajlm-269	178	7	-	-	PUNCT
ajlm-269	178	8	rna	rna	NOUN
ajlm-269	178	9	by	by	ADP
ajlm-269	178	10	real	real	ADJ
ajlm-269	178	11	time	time	NOUN
ajlm-269	178	12	pcr	pcr	NOUN
ajlm-269	178	13	using	use	VERB
ajlm-269	178	14	sybrgreen	sybrgreen	NOUN
ajlm-269	178	15	dye	dye	NOUN
ajlm-269	178	16	i.	i.	PROPN
ajlm-269	178	17	int	int	PROPN
ajlm-269	179	1	j	j	PROPN
ajlm-269	180	1	adv	adv	PROPN
ajlm-269	180	2	res	re	NOUN
ajlm-269	180	3	.	.	PUNCT
ajlm-269	181	1	2015;3(6):1450–1458	2015;3(6):1450–1458	ADJ
ajlm-269	181	2	.	.	PUNCT
ajlm-269	182	1	bennett	bennett	PROPN
ajlm-269	182	2	s	s	PROPN
ajlm-269	182	3	,	,	PUNCT
ajlm-269	182	4	gunson	gunson	PROPN
ajlm-269	182	5	rn	rn	PROPN
ajlm-269	182	6	,	,	PUNCT
ajlm-269	182	7	mcallister	mcallister	PROPN
ajlm-269	182	8	ge	ge	PROPN
ajlm-269	182	9	,	,	PUNCT
ajlm-269	182	10	et	et	PROPN
ajlm-269	182	11	al	al	PROPN
ajlm-269	182	12	.	.	PUNCT
ajlm-269	182	13	detection	detection	NOUN
ajlm-269	182	14	of	of	ADP
ajlm-269	182	15	hepatitis	hepatitis	PROPN
ajlm-269	182	16	c	c	PROPN
ajlm-269	182	17	virus	virus	PROPN
ajlm-269	182	18	rna	rna	PROPN
ajlm-269	182	19	in	in	ADP
ajlm-269	182	20	dried	dry	VERB
ajlm-269	182	21	blood	blood	NOUN
ajlm-269	182	22	spots	spot	NOUN
ajlm-269	182	23	.	.	PUNCT
ajlm-269	183	1	j	j	PROPN
ajlm-269	183	2	clin	clin	PROPN
ajlm-269	183	3	virol	virol	PROPN
ajlm-269	183	4	.	.	PUNCT
ajlm-269	184	1	2012;54(2):106–109	2012;54(2):106–109	X
ajlm-269	184	2	.	.	PUNCT
ajlm-269	184	3	http://dx.doi.org/10.1016/j.jcv.2012.02.004	http://dx.doi.org/10.1016/j.jcv.2012.02.004	PROPN
ajlm-269	184	4	de	de	X
ajlm-269	184	5	crignis	crignis	PROPN
ajlm-269	184	6	e	e	PROPN
ajlm-269	184	7	,	,	PUNCT
ajlm-269	184	8	re	re	PROPN
ajlm-269	184	9	mc	mc	PROPN
ajlm-269	184	10	,	,	PUNCT
ajlm-269	184	11	cimatti	cimatti	PROPN
ajlm-269	184	12	l	l	PROPN
ajlm-269	184	13	,	,	PUNCT
ajlm-269	184	14	et	et	PROPN
ajlm-269	184	15	al	al	PROPN
ajlm-269	184	16	.	.	PROPN
ajlm-269	184	17	hiv	hiv	PROPN
ajlm-269	184	18	and	and	CCONJ
ajlm-269	184	19	hcv	hcv	PROPN
ajlm-269	184	20	detection	detection	NOUN
ajlm-269	184	21	in	in	ADP
ajlm-269	184	22	dbs	dbs	NOUN
ajlm-269	184	23	by	by	ADP
ajlm-269	184	24	sybr	sybr	PROPN
ajlm-269	184	25	green	green	PROPN
ajlm-269	184	26	multiplex	multiplex	PROPN
ajlm-269	184	27	real	real	ADJ
ajlm-269	184	28	time	time	NOUN
ajlm-269	184	29	rt	rt	PROPN
ajlm-269	184	30	-	-	PUNCT
ajlm-269	184	31	pcr	pcr	NOUN
ajlm-269	184	32	.	.	PUNCT
ajlm-269	185	1	j	j	PROPN
ajlm-269	185	2	virol	virol	NOUN
ajlm-269	185	3	methods	method	NOUN
ajlm-269	185	4	.	.	PUNCT
ajlm-269	186	1	2010;165(1):51–56	2010;165(1):51–56	X
ajlm-269	186	2	.	.	PUNCT
ajlm-269	187	1	http://dx.doi.org/10.1016/j.jviromet.2009.12.017	http://dx.doi.org/10.1016/j.jviromet.2009.12.017	PROPN
ajlm-269	187	2	hope	hope	NOUN
ajlm-269	187	3	vd	vd	NOUN
ajlm-269	187	4	,	,	PUNCT
ajlm-269	187	5	hickman	hickman	PROPN
ajlm-269	187	6	m	m	PROPN
ajlm-269	187	7	,	,	PUNCT
ajlm-269	187	8	ngui	ngui	PROPN
ajlm-269	187	9	sl	sl	PROPN
ajlm-269	187	10	,	,	PUNCT
ajlm-269	187	11	et	et	PROPN
ajlm-269	187	12	al	al	PROPN
ajlm-269	187	13	.	.	PROPN
ajlm-269	187	14	measuring	measure	VERB
ajlm-269	187	15	the	the	DET
ajlm-269	187	16	incidence	incidence	NOUN
ajlm-269	187	17	,	,	PUNCT
ajlm-269	187	18	prevalence	prevalence	NOUN
ajlm-269	187	19	and	and	CCONJ
ajlm-269	187	20	genetic	genetic	ADJ
ajlm-269	187	21	relatedness	relatedness	NOUN
ajlm-269	187	22	of	of	ADP
ajlm-269	187	23	hepatitis	hepatitis	PROPN
ajlm-269	187	24	c	c	PROPN
ajlm-269	187	25	infections	infection	NOUN
ajlm-269	187	26	among	among	ADP
ajlm-269	187	27	a	a	DET
ajlm-269	187	28	community	community	NOUN
ajlm-269	187	29	recruited	recruit	VERB
ajlm-269	187	30	sample	sample	NOUN
ajlm-269	187	31	of	of	ADP
ajlm-269	187	32	injecting	inject	VERB
ajlm-269	187	33	drug	drug	NOUN
ajlm-269	187	34	users	user	NOUN
ajlm-269	187	35	,	,	PUNCT
ajlm-269	187	36	using	use	VERB
ajlm-269	187	37	dried	dry	VERB
ajlm-269	187	38	blood	blood	NOUN
ajlm-269	187	39	spots	spot	NOUN
ajlm-269	187	40	.	.	PUNCT
ajlm-269	188	1	j	j	PROPN
ajlm-269	188	2	viral	viral	ADJ
ajlm-269	188	3	hepat	hepat	X
ajlm-269	188	4	.	.	PUNCT
ajlm-269	189	1	2011;18(4):262–270	2011;18(4):262–270	X
ajlm-269	189	2	.	.	PUNCT
ajlm-269	190	1	http://dx.doi.org/10.1111/j.1365-2893.2010.01297.x	http://dx.doi.org/10.1111/j.1365-2893.2010.01297.x	NOUN
ajlm-269	190	2	santos	santos	PROPN
ajlm-269	190	3	c	c	PROPN
ajlm-269	190	4	,	,	PUNCT
ajlm-269	190	5	reis	reis	NOUN
ajlm-269	190	6	a	a	PRON
ajlm-269	190	7	,	,	PUNCT
ajlm-269	190	8	dos	dos	PROPN
ajlm-269	190	9	santos	santos	PROPN
ajlm-269	190	10	cv	cv	PROPN
ajlm-269	190	11	,	,	PUNCT
ajlm-269	190	12	et	et	PROPN
ajlm-269	190	13	al	al	PROPN
ajlm-269	190	14	.	.	PUNCT
ajlm-269	191	1	the	the	DET
ajlm-269	191	2	use	use	NOUN
ajlm-269	191	3	of	of	ADP
ajlm-269	191	4	real	real	ADJ
ajlm-269	191	5	-	-	PUNCT
ajlm-269	191	6	time	time	NOUN
ajlm-269	191	7	pcr	pcr	NOUN
ajlm-269	191	8	to	to	PART
ajlm-269	191	9	detect	detect	VERB
ajlm-269	191	10	hepatitis	hepatitis	PROPN
ajlm-269	191	11	c	c	PROPN
ajlm-269	191	12	virus	virus	NOUN
ajlm-269	191	13	rna	rna	PROPN
ajlm-269	191	14	in	in	ADP
ajlm-269	191	15	dried	dry	VERB
ajlm-269	191	16	blood	blood	NOUN
ajlm-269	191	17	spots	spot	NOUN
ajlm-269	191	18	from	from	ADP
ajlm-269	191	19	brazilian	brazilian	ADJ
ajlm-269	191	20	patients	patient	NOUN
ajlm-269	191	21	infected	infect	VERB
ajlm-269	191	22	chronically	chronically	ADV
ajlm-269	191	23	.	.	PUNCT
ajlm-269	192	1	j	j	PROPN
ajlm-269	192	2	virol	virol	PROPN
ajlm-269	192	3	methods	method	NOUN
ajlm-269	192	4	.	.	PUNCT
ajlm-269	193	1	2011;179(1):17–20	2011;179(1):17–20	X
ajlm-269	193	2	.	.	PUNCT
ajlm-269	194	1	http://dx.doi.org/10.1016/j.jviromet.2011.06.012	http://dx.doi.org/10.1016/j.jviromet.2011.06.012	PROPN
ajlm-269	194	2	tuaillon	tuaillon	NOUN
ajlm-269	194	3	e	e	NOUN
ajlm-269	194	4	,	,	PUNCT
ajlm-269	194	5	mondain	mondain	PROPN
ajlm-269	194	6	a	a	PRON
ajlm-269	194	7	,	,	PUNCT
ajlm-269	194	8	meroueh	meroueh	PROPN
ajlm-269	194	9	f	f	AUX
ajlm-269	194	10	,	,	PUNCT
ajlm-269	194	11	et	et	PROPN
ajlm-269	194	12	al	al	PROPN
ajlm-269	194	13	.	.	PROPN
ajlm-269	195	1	dried	dry	VERB
ajlm-269	195	2	blood	blood	NOUN
ajlm-269	195	3	spot	spot	NOUN
ajlm-269	195	4	for	for	ADP
ajlm-269	195	5	hepatitis	hepatitis	PROPN
ajlm-269	195	6	c	c	PROPN
ajlm-269	195	7	virus	virus	NOUN
ajlm-269	195	8	serology	serology	NOUN
ajlm-269	195	9	and	and	CCONJ
ajlm-269	195	10	molecular	molecular	ADJ
ajlm-269	195	11	testing	testing	NOUN
ajlm-269	195	12	.	.	PUNCT
ajlm-269	196	1	hepatology	hepatology	NOUN
ajlm-269	196	2	.	.	PUNCT
ajlm-269	197	1	2010;51(3):752–758	2010;51(3):752–758	NUM
ajlm-269	197	2	.	.	PUNCT
ajlm-269	197	3	soulier	soulier	PROPN
ajlm-269	197	4	a	a	PROPN
ajlm-269	197	5	,	,	PUNCT
ajlm-269	197	6	poiteau	poiteau	PROPN
ajlm-269	197	7	l	l	PROPN
ajlm-269	197	8	,	,	PUNCT
ajlm-269	197	9	rosa	rosa	PROPN
ajlm-269	197	10	i	i	PROPN
ajlm-269	197	11	,	,	PUNCT
ajlm-269	197	12	et	et	PROPN
ajlm-269	197	13	al	al	PROPN
ajlm-269	197	14	.	.	PROPN
ajlm-269	197	15	dried	dry	VERB
ajlm-269	197	16	blood	blood	NOUN
ajlm-269	197	17	spots	spot	NOUN
ajlm-269	197	18	:	:	PUNCT
ajlm-269	197	19	a	a	DET
ajlm-269	197	20	tool	tool	NOUN
ajlm-269	197	21	to	to	PART
ajlm-269	197	22	ensure	ensure	VERB
ajlm-269	197	23	broad	broad	ADJ
ajlm-269	197	24	access	access	NOUN
ajlm-269	197	25	to	to	ADP
ajlm-269	197	26	hepatitis	hepatitis	PROPN
ajlm-269	197	27	c	c	NOUN
ajlm-269	197	28	screening	screening	NOUN
ajlm-269	197	29	,	,	PUNCT
ajlm-269	197	30	diagnosis	diagnosis	NOUN
ajlm-269	197	31	,	,	PUNCT
ajlm-269	197	32	and	and	CCONJ
ajlm-269	197	33	treatment	treatment	NOUN
ajlm-269	197	34	monitoring	monitoring	NOUN
ajlm-269	197	35	.	.	PUNCT
ajlm-269	198	1	j	j	PROPN
ajlm-269	198	2	infect	infect	VERB
ajlm-269	198	3	dis	dis	PROPN
ajlm-269	198	4	.	.	PUNCT
ajlm-269	199	1	2015	2015	NUM
ajlm-269	199	2	sep	sep	NOUN
ajlm-269	199	3	2	2	NUM
ajlm-269	199	4	.	.	PUNCT
ajlm-269	199	5	pii	pii	PROPN
ajlm-269	199	6	:	:	PUNCT
ajlm-269	199	7	jiv423	jiv423	X
ajlm-269	199	8	.	.	PUNCT
ajlm-269	200	1	epub	epub	NOUN
ajlm-269	200	2	ahead	ahead	ADV
ajlm-269	200	3	of	of	ADP
ajlm-269	200	4	print	print	NOUN
ajlm-269	200	5	.	.	PUNCT
ajlm-269	201	1	http://dx.doi.org/10.1093/infdis/jiv423	http://dx.doi.org/10.1093/infdis/jiv423	PROPN
ajlm-269	201	2	mutagi	mutagi	PROPN
ajlm-269	201	3	a	a	PROPN
ajlm-269	201	4	,	,	PUNCT
ajlm-269	201	5	atherton	atherton	PROPN
ajlm-269	201	6	c	c	PROPN
ajlm-269	201	7	,	,	PUNCT
ajlm-269	201	8	wilson	wilson	PROPN
ajlm-269	201	9	s	s	PROPN
ajlm-269	201	10	,	,	PUNCT
ajlm-269	201	11	et	et	PROPN
ajlm-269	201	12	al	al	PROPN
ajlm-269	201	13	.	.	PUNCT
ajlm-269	201	14	development	development	NOUN
ajlm-269	201	15	of	of	ADP
ajlm-269	201	16	qualitative	qualitative	ADJ
ajlm-269	201	17	hcv	hcv	PROPN
ajlm-269	201	18	rna	rna	NOUN
ajlm-269	201	19	testing	testing	NOUN
ajlm-269	201	20	on	on	ADP
ajlm-269	201	21	dried	dry	VERB
ajlm-269	201	22	blood	blood	NOUN
ajlm-269	201	23	spots	spot	NOUN
ajlm-269	201	24	as	as	ADP
ajlm-269	201	25	an	an	DET
ajlm-269	201	26	adjunct	adjunct	ADJ
ajlm-269	201	27	screening	screening	NOUN
ajlm-269	201	28	tool	tool	NOUN
ajlm-269	201	29	to	to	PART
ajlm-269	201	30	identify	identify	VERB
ajlm-269	201	31	active	active	ADJ
ajlm-269	201	32	hepatitis	hepatitis	NOUN
ajlm-269	201	33	c	c	NOUN
ajlm-269	201	34	infection	infection	NOUN
ajlm-269	201	35	.	.	PUNCT
ajlm-269	202	1	open	open	ADJ
ajlm-269	202	2	forum	forum	PROPN
ajlm-269	202	3	infect	infect	VERB
ajlm-269	202	4	dis	dis	PROPN
ajlm-269	202	5	.	.	PUNCT
ajlm-269	203	1	2014;1(suppl	2014;1(suppl	NUM
ajlm-269	203	2	1):s376	1):s376	NUM
ajlm-269	203	3	–	–	PUNCT
ajlm-269	203	4	s377	s377	NUM
ajlm-269	203	5	.	.	PUNCT
ajlm-269	204	1	fouad	fouad	PROPN
ajlm-269	204	2	na	na	PROPN
ajlm-269	204	3	,	,	PUNCT
ajlm-269	204	4	mahedy	mahedy	PROPN
ajlm-269	204	5	aw	aw	INTJ
ajlm-269	204	6	,	,	PUNCT
ajlm-269	204	7	el	el	PROPN
ajlm-269	204	8	-	-	PROPN
ajlm-269	204	9	taher	taher	PROPN
ajlm-269	204	10	sm	sm	PROPN
ajlm-269	204	11	.	.	PUNCT
ajlm-269	204	12	application	application	NOUN
ajlm-269	204	13	of	of	ADP
ajlm-269	204	14	dried	dry	VERB
ajlm-269	204	15	blood	blood	NOUN
ajlm-269	204	16	spot	spot	NOUN
ajlm-269	204	17	testing	testing	NOUN
ajlm-269	204	18	for	for	ADP
ajlm-269	204	19	hepatitis	hepatitis	PROPN
ajlm-269	204	20	c	c	PROPN
ajlm-269	204	21	virus	virus	NOUN
ajlm-269	204	22	rna	rna	NOUN
ajlm-269	204	23	amplification	amplification	NOUN
ajlm-269	204	24	.	.	PUNCT
ajlm-269	205	1	egypt	egypt	PROPN
ajlm-269	205	2	j	j	PROPN
ajlm-269	205	3	med	med	PROPN
ajlm-269	205	4	microbiol	microbiol	PROPN
ajlm-269	205	5	.	.	PUNCT
ajlm-269	206	1	2013;22(11):1–8	2013;22(11):1–8	X
ajlm-269	206	2	.	.	PUNCT
ajlm-269	207	1	http://dx.doi.org/10.12816/0004921	http://dx.doi.org/10.12816/0004921	PROPN
ajlm-269	207	2	ross	ross	PROPN
ajlm-269	207	3	rs	rs	PROPN
ajlm-269	207	4	,	,	PUNCT
ajlm-269	207	5	stambouli	stambouli	ADJ
ajlm-269	207	6	o	o	NOUN
ajlm-269	207	7	,	,	PUNCT
ajlm-269	207	8	grüner	grüner	PROPN
ajlm-269	207	9	n	n	CCONJ
ajlm-269	207	10	,	,	PUNCT
ajlm-269	207	11	et	et	PROPN
ajlm-269	207	12	al	al	PROPN
ajlm-269	207	13	.	.	PUNCT
ajlm-269	207	14	detection	detection	NOUN
ajlm-269	207	15	of	of	ADP
ajlm-269	207	16	infections	infection	NOUN
ajlm-269	207	17	with	with	ADP
ajlm-269	207	18	hepatitis	hepatitis	PROPN
ajlm-269	207	19	b	b	PROPN
ajlm-269	207	20	virus	virus	NOUN
ajlm-269	207	21	,	,	PUNCT
ajlm-269	207	22	hepatitis	hepatitis	PROPN
ajlm-269	207	23	c	c	PROPN
ajlm-269	207	24	virus	virus	NOUN
ajlm-269	207	25	,	,	PUNCT
ajlm-269	207	26	and	and	CCONJ
ajlm-269	207	27	human	human	ADJ
ajlm-269	207	28	immunodeficiency	immunodeficiency	NOUN
ajlm-269	207	29	virus	virus	NOUN
ajlm-269	207	30	by	by	ADP
ajlm-269	207	31	analyses	analysis	NOUN
ajlm-269	207	32	of	of	ADP
ajlm-269	207	33	dried	dry	VERB
ajlm-269	207	34	blood	blood	NOUN
ajlm-269	207	35	spots	spot	NOUN
ajlm-269	207	36	–	–	PUNCT
ajlm-269	207	37	performance	performance	NOUN
ajlm-269	207	38	characteristics	characteristic	NOUN
ajlm-269	207	39	of	of	ADP
ajlm-269	207	40	the	the	DET
ajlm-269	207	41	architect	architect	NOUN
ajlm-269	207	42	system	system	NOUN
ajlm-269	207	43	and	and	CCONJ
ajlm-269	207	44	two	two	NUM
ajlm-269	207	45	commercial	commercial	ADJ
ajlm-269	207	46	assays	assay	NOUN
ajlm-269	207	47	for	for	ADP
ajlm-269	207	48	nucleic	nucleic	ADJ
ajlm-269	207	49	acid	acid	NOUN
ajlm-269	207	50	amplification	amplification	NOUN
ajlm-269	207	51	.	.	PUNCT
ajlm-269	208	1	virol	virol	NOUN
ajlm-269	208	2	j.	j.	PROPN
ajlm-269	208	3	2013;10:72	2013;10:72	PROPN
ajlm-269	208	4	.	.	PUNCT
ajlm-269	209	1	http://dx.doi.org/10.1186/1743-422x-10-72	http://dx.doi.org/10.1186/1743-422x-10-72	ADJ
ajlm-269	209	2	moodley	moodley	NOUN
ajlm-269	209	3	p	p	NOUN
ajlm-269	209	4	,	,	PUNCT
ajlm-269	209	5	parboosing	parboose	VERB
ajlm-269	209	6	r	r	NOUN
ajlm-269	209	7	,	,	PUNCT
ajlm-269	209	8	moodley	moodley	PROPN
ajlm-269	209	9	d.	d.	PROPN
ajlm-269	209	10	reduction	reduction	NOUN
ajlm-269	209	11	in	in	ADP
ajlm-269	209	12	perinatal	perinatal	ADJ
ajlm-269	209	13	hiv	hiv	PROPN
ajlm-269	209	14	infections	infection	NOUN
ajlm-269	209	15	in	in	ADP
ajlm-269	209	16	kwazulu	kwazulu	PROPN
ajlm-269	209	17	-	-	PUNCT
ajlm-269	209	18	natal	natal	ADJ
ajlm-269	209	19	,	,	PUNCT
ajlm-269	209	20	south	south	PROPN
ajlm-269	209	21	africa	africa	PROPN
ajlm-269	209	22	,	,	PUNCT
ajlm-269	209	23	in	in	ADP
ajlm-269	209	24	the	the	DET
ajlm-269	209	25	era	era	NOUN
ajlm-269	209	26	of	of	ADP
ajlm-269	209	27	more	more	ADV
ajlm-269	209	28	effective	effective	ADJ
ajlm-269	209	29	prevention	prevention	NOUN
ajlm-269	209	30	of	of	ADP
ajlm-269	209	31	mother	mother	NOUN
ajlm-269	209	32	to	to	ADP
ajlm-269	209	33	child	child	NOUN
ajlm-269	209	34	transmission	transmission	NOUN
ajlm-269	209	35	interventions	intervention	NOUN
ajlm-269	209	36	(	(	PUNCT
ajlm-269	209	37	2004–2012	2004–2012	NUM
ajlm-269	209	38	)	)	PUNCT
ajlm-269	209	39	.	.	PUNCT
ajlm-269	210	1	j	j	PROPN
ajlm-269	210	2	acquir	acquir	PROPN
ajlm-269	210	3	immune	immune	VERB
ajlm-269	210	4	defic	defic	ADJ
ajlm-269	210	5	syndr	syndr	NOUN
ajlm-269	210	6	.	.	PUNCT
ajlm-269	211	1	2013;63(3):410–415	2013;63(3):410–415	NUM
ajlm-269	211	2	.	.	PUNCT
ajlm-269	212	1	http://dx.doi.org/10.1097/qai.0b013e3182926931	http://dx.doi.org/10.1097/qai.0b013e3182926931	PROPN
ajlm-269	212	2	young	young	PROPN
ajlm-269	212	3	ky	ky	PROPN
ajlm-269	212	4	,	,	PUNCT
ajlm-269	212	5	resnick	resnick	PROPN
ajlm-269	212	6	rm	rm	PROPN
ajlm-269	212	7	,	,	PUNCT
ajlm-269	212	8	myers	myers	PROPN
ajlm-269	212	9	tw	tw	PROPN
ajlm-269	212	10	.	.	PUNCT
ajlm-269	212	11	detection	detection	NOUN
ajlm-269	212	12	of	of	ADP
ajlm-269	212	13	hepatitis	hepatitis	PROPN
ajlm-269	212	14	c	c	PROPN
ajlm-269	212	15	virus	virus	PROPN
ajlm-269	212	16	rna	rna	PROPN
ajlm-269	212	17	by	by	ADP
ajlm-269	212	18	a	a	DET
ajlm-269	212	19	combined	combine	VERB
ajlm-269	212	20	reverse	reverse	ADJ
ajlm-269	212	21	transcription	transcription	NOUN
ajlm-269	212	22	-	-	PUNCT
ajlm-269	212	23	polymerase	polymerase	NOUN
ajlm-269	212	24	chain	chain	NOUN
ajlm-269	212	25	reaction	reaction	NOUN
ajlm-269	212	26	assay	assay	VERB
ajlm-269	212	27	.	.	PUNCT
ajlm-269	213	1	j	j	PROPN
ajlm-269	213	2	clin	clin	PROPN
ajlm-269	213	3	micro	micro	PROPN
ajlm-269	213	4	.	.	PROPN
ajlm-269	214	1	1993;31(4):882–886	1993;31(4):882–886	X
ajlm-269	214	2	.	.	PUNCT
ajlm-269	215	1	rapley	rapley	PROPN
ajlm-269	215	2	r	r	NOUN
ajlm-269	215	3	,	,	PUNCT
ajlm-269	215	4	whitehouse	whitehouse	NOUN
ajlm-269	215	5	d	d	NOUN
ajlm-269	215	6	,	,	PUNCT
ajlm-269	215	7	editors	editor	NOUN
ajlm-269	215	8	.	.	PUNCT
ajlm-269	216	1	molecular	molecular	ADJ
ajlm-269	216	2	biology	biology	NOUN
ajlm-269	216	3	and	and	CCONJ
ajlm-269	216	4	biotechnology	biotechnology	NOUN
ajlm-269	216	5	.	.	PUNCT
ajlm-269	217	1	6th	6th	ADJ
ajlm-269	217	2	ed	ed	NOUN
ajlm-269	217	3	.	.	PUNCT
ajlm-269	218	1	cambridge	cambridge	PROPN
ajlm-269	218	2	,	,	PUNCT
ajlm-269	218	3	united	united	ADJ
ajlm-269	218	4	kingdom	kingdom	PROPN
ajlm-269	218	5	:	:	PUNCT
ajlm-269	218	6	royal	royal	ADJ
ajlm-269	218	7	society	society	NOUN
ajlm-269	218	8	of	of	ADP
ajlm-269	218	9	chemistry	chemistry	NOUN
ajlm-269	218	10	;	;	PUNCT
ajlm-269	218	11	2014	2014	NUM
ajlm-269	218	12	.	.	PUNCT
ajlm-269	219	1	mcgovern	mcgovern	PROPN
ajlm-269	219	2	bh	bh	PROPN
ajlm-269	219	3	,	,	PUNCT
ajlm-269	219	4	birch	birch	PROPN
ajlm-269	219	5	ce	ce	PROPN
ajlm-269	219	6	,	,	PUNCT
ajlm-269	219	7	bowen	bowen	PROPN
ajlm-269	219	8	mj	mj	PROPN
ajlm-269	219	9	,	,	PUNCT
ajlm-269	219	10	et	et	PROPN
ajlm-269	219	11	al	al	PROPN
ajlm-269	219	12	.	.	PUNCT
ajlm-269	220	1	improving	improve	VERB
ajlm-269	220	2	the	the	DET
ajlm-269	220	3	diagnosis	diagnosis	NOUN
ajlm-269	220	4	of	of	ADP
ajlm-269	220	5	acute	acute	ADJ
ajlm-269	220	6	hepatitis	hepatitis	PROPN
ajlm-269	220	7	c	c	PROPN
ajlm-269	220	8	virus	virus	NOUN
ajlm-269	220	9	infection	infection	NOUN
ajlm-269	220	10	with	with	ADP
ajlm-269	220	11	expanded	expand	VERB
ajlm-269	220	12	viral	viral	ADJ
ajlm-269	220	13	load	load	NOUN
ajlm-269	220	14	criteria	criterion	NOUN
ajlm-269	220	15	.	.	PUNCT
ajlm-269	221	1	clin	clin	PROPN
ajlm-269	221	2	infect	infect	PROPN
ajlm-269	221	3	dis	dis	PROPN
ajlm-269	221	4	.	.	PUNCT
ajlm-269	222	1	2009;49(7):1051–1060	2009;49(7):1051–1060	NOUN
ajlm-269	222	2	.	.	PUNCT
ajlm-269	223	1	http://dx.doi.org/10.1086/605561	http://dx.doi.org/10.1086/605561	PROPN
ajlm-269	223	2	greenman	greenman	PROPN
ajlm-269	223	3	j	j	PROPN
ajlm-269	223	4	,	,	PUNCT
ajlm-269	223	5	roberts	roberts	PROPN
ajlm-269	223	6	t	t	PROPN
ajlm-269	223	7	,	,	PUNCT
ajlm-269	223	8	cohn	cohn	PROPN
ajlm-269	223	9	j	j	PROPN
ajlm-269	223	10	,	,	PUNCT
ajlm-269	223	11	et	et	PROPN
ajlm-269	223	12	al	al	PROPN
ajlm-269	223	13	.	.	PROPN
ajlm-269	223	14	dried	dry	VERB
ajlm-269	223	15	blood	blood	NOUN
ajlm-269	223	16	spot	spot	NOUN
ajlm-269	223	17	in	in	ADP
ajlm-269	223	18	the	the	DET
ajlm-269	223	19	genotyping	genotyping	NOUN
ajlm-269	223	20	,	,	PUNCT
ajlm-269	223	21	quantification	quantification	NOUN
ajlm-269	223	22	and	and	CCONJ
ajlm-269	223	23	storage	storage	NOUN
ajlm-269	223	24	of	of	ADP
ajlm-269	223	25	hcv	hcv	PROPN
ajlm-269	223	26	rna	rna	PROPN
ajlm-269	223	27	:	:	PUNCT
ajlm-269	223	28	a	a	DET
ajlm-269	223	29	systematic	systematic	ADJ
ajlm-269	223	30	literature	literature	NOUN
ajlm-269	223	31	review	review	NOUN
ajlm-269	223	32	.	.	PUNCT
ajlm-269	224	1	j	j	PROPN
ajlm-269	224	2	viral	viral	ADJ
ajlm-269	224	3	hepat	hepat	NOUN
ajlm-269	224	4	.	.	PUNCT
ajlm-269	225	1	2015;22(4):353–361	2015;22(4):353–361	X
ajlm-269	225	2	.	.	PUNCT
ajlm-269	226	1	http://dx.doi.org/10.1111/jvh.12345	http://dx.doi.org/10.1111/jvh.12345	PROPN
ajlm-269	226	2	snijdewind	snijdewind	PROPN
ajlm-269	226	3	ijm	ijm	PROPN
ajlm-269	226	4	,	,	PUNCT
ajlm-269	226	5	van	van	PROPN
ajlm-269	226	6	kampen	kampen	PROPN
ajlm-269	226	7	jja	jja	PROPN
ajlm-269	226	8	,	,	PUNCT
ajlm-269	226	9	fraaij	fraaij	NOUN
ajlm-269	226	10	pla	pla	NOUN
ajlm-269	226	11	,	,	PUNCT
ajlm-269	226	12	et	et	PROPN
ajlm-269	226	13	al	al	PROPN
ajlm-269	226	14	.	.	PROPN
ajlm-269	227	1	current	current	ADJ
ajlm-269	227	2	and	and	CCONJ
ajlm-269	227	3	future	future	ADJ
ajlm-269	227	4	applications	application	NOUN
ajlm-269	227	5	of	of	ADP
ajlm-269	227	6	dried	dry	VERB
ajlm-269	227	7	blood	blood	NOUN
ajlm-269	227	8	spots	spot	NOUN
ajlm-269	227	9	in	in	ADP
ajlm-269	227	10	viral	viral	ADJ
ajlm-269	227	11	disease	disease	NOUN
ajlm-269	227	12	management	management	NOUN
ajlm-269	227	13	.	.	PUNCT
ajlm-269	228	1	antiviral	antiviral	ADJ
ajlm-269	228	2	res	re	NOUN
ajlm-269	228	3	.	.	PUNCT
ajlm-269	229	1	2012;93(3):309–321	2012;93(3):309–321	X
ajlm-269	229	2	.	.	PUNCT
ajlm-269	230	1	http://dx.doi.org/10.1016/j.antiviral.2011.12.011	http://dx.doi.org/10.1016/j.antiviral.2011.12.011	PROPN
ajlm-269	230	2	hickman	hickman	PROPN
ajlm-269	230	3	m	m	PROPN
ajlm-269	230	4	,	,	PUNCT
ajlm-269	230	5	mcdonald	mcdonald	PROPN
ajlm-269	230	6	t	t	PROPN
ajlm-269	230	7	,	,	PUNCT
ajlm-269	230	8	judd	judd	PROPN
ajlm-269	230	9	a	a	PROPN
ajlm-269	230	10	,	,	PUNCT
ajlm-269	230	11	et	et	PROPN
ajlm-269	230	12	al	al	PROPN
ajlm-269	230	13	.	.	PUNCT
ajlm-269	231	1	increasing	increase	VERB
ajlm-269	231	2	the	the	DET
ajlm-269	231	3	uptake	uptake	NOUN
ajlm-269	231	4	of	of	ADP
ajlm-269	231	5	hepatitis	hepatitis	PROPN
ajlm-269	231	6	c	c	PROPN
ajlm-269	231	7	virus	virus	NOUN
ajlm-269	231	8	testing	testing	NOUN
ajlm-269	231	9	among	among	ADP
ajlm-269	231	10	injecting	inject	VERB
ajlm-269	231	11	drug	drug	NOUN
ajlm-269	231	12	users	user	NOUN
ajlm-269	231	13	in	in	ADP
ajlm-269	231	14	specialist	specialist	ADJ
ajlm-269	231	15	drug	drug	NOUN
ajlm-269	231	16	treatment	treatment	NOUN
ajlm-269	231	17	and	and	CCONJ
ajlm-269	231	18	prison	prison	NOUN
ajlm-269	231	19	settings	setting	NOUN
ajlm-269	231	20	by	by	ADP
ajlm-269	231	21	using	use	VERB
ajlm-269	231	22	dried	dry	VERB
ajlm-269	231	23	blood	blood	NOUN
ajlm-269	231	24	spots	spot	NOUN
ajlm-269	231	25	for	for	ADP
ajlm-269	231	26	diagnostic	diagnostic	ADJ
ajlm-269	231	27	testing	testing	NOUN
ajlm-269	231	28	:	:	PUNCT
ajlm-269	231	29	a	a	DET
ajlm-269	231	30	cluster	cluster	NOUN
ajlm-269	231	31	randomized	randomize	VERB
ajlm-269	231	32	control	control	NOUN
ajlm-269	231	33	trial	trial	NOUN
ajlm-269	231	34	.	.	PUNCT
ajlm-269	232	1	j	j	PROPN
ajlm-269	232	2	viral	viral	ADJ
ajlm-269	232	3	hepat	hepat	NOUN
ajlm-269	232	4	.	.	PUNCT
ajlm-269	233	1	2008;15(4):250–254	2008;15(4):250–254	X
ajlm-269	233	2	.	.	PUNCT
ajlm-269	234	1	http://dx.doi.org/10.1111/j.1365-2893.2007.00937.x	http://dx.doi.org/10.1111/j.1365-2893.2007.00937.x	NOUN
ajlm-269	234	2	njouom	njouom	NOUN
ajlm-269	234	3	r	r	NOUN
ajlm-269	234	4	,	,	PUNCT
ajlm-269	234	5	pasquier	pasquier	NOUN
ajlm-269	234	6	c	c	PROPN
ajlm-269	234	7	,	,	PUNCT
ajlm-269	234	8	ayouba	ayouba	VERB
ajlm-269	234	9	a	a	X
ajlm-269	234	10	,	,	PUNCT
ajlm-269	234	11	et	et	PROPN
ajlm-269	234	12	al	al	PROPN
ajlm-269	234	13	.	.	PUNCT
ajlm-269	234	14	low	low	ADJ
ajlm-269	234	15	risk	risk	NOUN
ajlm-269	234	16	of	of	ADP
ajlm-269	234	17	mother	mother	NOUN
ajlm-269	234	18	-	-	PUNCT
ajlm-269	234	19	to	to	ADP
ajlm-269	234	20	-	-	PUNCT
ajlm-269	234	21	child	child	NOUN
ajlm-269	234	22	-	-	PUNCT
ajlm-269	234	23	transmission	transmission	NOUN
ajlm-269	234	24	of	of	ADP
ajlm-269	234	25	hepatitis	hepatitis	PROPN
ajlm-269	234	26	c	c	PROPN
ajlm-269	234	27	virus	virus	PROPN
ajlm-269	234	28	in	in	ADP
ajlm-269	234	29	yaounde	yaounde	PROPN
ajlm-269	234	30	,	,	PUNCT
ajlm-269	234	31	cameroon	cameroon	PROPN
ajlm-269	234	32	:	:	PUNCT
ajlm-269	234	33	the	the	DET
ajlm-269	234	34	anrs	anrs	NOUN
ajlm-269	234	35	1262	1262	NUM
ajlm-269	234	36	study	study	NOUN
ajlm-269	234	37	.	.	PUNCT
ajlm-269	235	1	am	be	AUX
ajlm-269	235	2	j	j	PROPN
ajlm-269	235	3	trop	trop	X
ajlm-269	235	4	med	med	PROPN
ajlm-269	235	5	hyg	hyg	PROPN
ajlm-269	235	6	.	.	PUNCT
ajlm-269	236	1	2005;73(2):460–466	2005;73(2):460–466	NUM
ajlm-269	236	2	.	.	PROPN
ajlm-269	236	3	ogboghodo	ogboghodo	PROPN
ajlm-269	236	4	bc	bc	PROPN
ajlm-269	236	5	,	,	PUNCT
ajlm-269	236	6	aigbirior	aigbirior	PROPN
ajlm-269	236	7	ma	ma	PROPN
ajlm-269	236	8	,	,	PUNCT
ajlm-269	236	9	bazuaye	bazuaye	VERB
ajlm-269	236	10	gn	gn	PROPN
ajlm-269	236	11	,	,	PUNCT
ajlm-269	236	12	et	et	PROPN
ajlm-269	236	13	al	al	PROPN
ajlm-269	236	14	.	.	PUNCT
ajlm-269	237	1	hepatitis	hepatitis	PROPN
ajlm-269	237	2	c	c	PROPN
ajlm-269	237	3	virus	virus	NOUN
ajlm-269	237	4	and	and	CCONJ
ajlm-269	237	5	human	human	ADJ
ajlm-269	237	6	immunodeficiency	immunodeficiency	NOUN
ajlm-269	237	7	virus	virus	NOUN
ajlm-269	237	8	-	-	PUNCT
ajlm-269	237	9	i	i	PRON
ajlm-269	237	10	(	(	PUNCT
ajlm-269	237	11	hiv	hiv	PROPN
ajlm-269	237	12	)	)	PUNCT
ajlm-269	237	13	co	co	NOUN
ajlm-269	237	14	-	-	NOUN
ajlm-269	237	15	infection	infection	NOUN
ajlm-269	237	16	in	in	ADP
ajlm-269	237	17	children	child	NOUN
ajlm-269	237	18	in	in	ADP
ajlm-269	237	19	benin	benin	PROPN
ajlm-269	237	20	city	city	PROPN
ajlm-269	237	21	,	,	PUNCT
ajlm-269	237	22	nigeria	nigeria	PROPN
ajlm-269	237	23	.	.	PUNCT
ajlm-269	238	1	afr	afr	PROPN
ajlm-269	238	2	.	.	PUNCT
ajlm-269	239	1	j.	j.	PROPN
ajlm-269	239	2	biomed	biome	VERB
ajlm-269	239	3	.	.	PUNCT
ajlm-269	240	1	res	re	NOUN
ajlm-269	240	2	.	.	PUNCT
ajlm-269	241	1	2009;12(1):1–6	2009;12(1):1–6	NUM
ajlm-269	241	2	.	.	PUNCT
ajlm-269	241	3	sadoh	sadoh	PROPN
ajlm-269	241	4	ae	ae	PROPN
ajlm-269	241	5	,	,	PUNCT
ajlm-269	241	6	sadoh	sadoh	PROPN
ajlm-269	241	7	we	we	PRON
ajlm-269	241	8	,	,	PUNCT
ajlm-269	241	9	iduoriyekemwen	iduoriyekemwen	PROPN
ajlm-269	241	10	nj	nj	PROPN
ajlm-269	241	11	.	.	PUNCT
ajlm-269	242	1	hiv	hiv	PROPN
ajlm-269	242	2	co	co	NOUN
ajlm-269	242	3	-	-	NOUN
ajlm-269	243	1	infection	infection	NOUN
ajlm-269	243	2	with	with	ADP
ajlm-269	243	3	hepatitis	hepatitis	PROPN
ajlm-269	243	4	b	b	PROPN
ajlm-269	243	5	and	and	CCONJ
ajlm-269	243	6	c	c	PROPN
ajlm-269	243	7	viruses	virus	NOUN
ajlm-269	243	8	among	among	ADP
ajlm-269	243	9	nigerian	nigerian	ADJ
ajlm-269	243	10	children	child	NOUN
ajlm-269	243	11	in	in	ADP
ajlm-269	243	12	an	an	DET
ajlm-269	243	13	antiretroviral	antiretroviral	ADJ
ajlm-269	243	14	treatment	treatment	NOUN
ajlm-269	243	15	programme	programme	NOUN
ajlm-269	243	16	.	.	PUNCT
ajlm-269	244	1	s.	s.	PROPN
ajlm-269	244	2	afr	afr	PROPN
ajlm-269	244	3	.	.	PUNCT
ajlm-269	245	1	j.	j.	PROPN
ajlm-269	245	2	child	child	PROPN
ajlm-269	245	3	health	health	PROPN
ajlm-269	245	4	.	.	PUNCT
ajlm-269	246	1	2011;5(1):7–10	2011;5(1):7–10	X
ajlm-269	246	2	.	.	PUNCT