id	sid	tid	token	lemma	pos
ajlm-609	1	1	abstract	abstract	ADJ
ajlm-609	1	2	introduction	introduction	NOUN
ajlm-609	1	3	methods	method	NOUN
ajlm-609	1	4	results	result	VERB
ajlm-609	1	5	discussion	discussion	NOUN
ajlm-609	1	6	trustworthiness	trustworthiness	NOUN
ajlm-609	1	7	acknowledgements	acknowledgement	NOUN
ajlm-609	1	8	references	reference	NOUN
ajlm-609	1	9	about	about	ADP
ajlm-609	1	10	the	the	DET
ajlm-609	1	11	author(s	author(s	NOUN
ajlm-609	1	12	)	)	PUNCT
ajlm-609	1	13	john	john	PROPN
ajlm-609	1	14	b.	b.	PROPN
ajlm-609	1	15	kalule	kalule	PROPN
ajlm-609	1	16	faculty	faculty	PROPN
ajlm-609	1	17	of	of	ADP
ajlm-609	1	18	health	health	NOUN
ajlm-609	1	19	sciences	sciences	PROPN
ajlm-609	1	20	,	,	PUNCT
ajlm-609	1	21	university	university	NOUN
ajlm-609	1	22	of	of	ADP
ajlm-609	1	23	cape	cape	PROPN
ajlm-609	1	24	town	town	NOUN
ajlm-609	1	25	,	,	PUNCT
ajlm-609	1	26	cape	cape	NOUN
ajlm-609	1	27	town	town	NOUN
ajlm-609	1	28	,	,	PUNCT
ajlm-609	1	29	south	south	PROPN
ajlm-609	1	30	africa	africa	PROPN
ajlm-609	1	31	karen	karen	PROPN
ajlm-609	1	32	h.	h.	PROPN
ajlm-609	1	33	keddy	keddy	PROPN
ajlm-609	1	34	center	center	PROPN
ajlm-609	1	35	for	for	ADP
ajlm-609	1	36	enteric	enteric	ADJ
ajlm-609	1	37	disease	disease	NOUN
ajlm-609	1	38	research	research	NOUN
ajlm-609	1	39	unit	unit	NOUN
ajlm-609	1	40	,	,	PUNCT
ajlm-609	1	41	national	national	PROPN
ajlm-609	1	42	institute	institute	PROPN
ajlm-609	1	43	for	for	ADP
ajlm-609	1	44	communicable	communicable	NOUN
ajlm-609	1	45	diseases	disease	NOUN
ajlm-609	1	46	,	,	PUNCT
ajlm-609	1	47	johannesburg	johannesburg	PROPN
ajlm-609	1	48	,	,	PUNCT
ajlm-609	1	49	south	south	PROPN
ajlm-609	1	50	africa	africa	PROPN
ajlm-609	1	51	anthony	anthony	PROPN
ajlm-609	1	52	smith	smith	PROPN
ajlm-609	1	53	center	center	PROPN
ajlm-609	1	54	for	for	ADP
ajlm-609	1	55	enteric	enteric	ADJ
ajlm-609	1	56	disease	disease	NOUN
ajlm-609	1	57	research	research	NOUN
ajlm-609	1	58	unit	unit	NOUN
ajlm-609	1	59	,	,	PUNCT
ajlm-609	1	60	national	national	PROPN
ajlm-609	1	61	institute	institute	PROPN
ajlm-609	1	62	for	for	ADP
ajlm-609	1	63	communicable	communicable	NOUN
ajlm-609	1	64	diseases	disease	NOUN
ajlm-609	1	65	,	,	PUNCT
ajlm-609	1	66	johannesburg	johannesburg	PROPN
ajlm-609	1	67	,	,	PUNCT
ajlm-609	1	68	south	south	PROPN
ajlm-609	1	69	africa	africa	PROPN
ajlm-609	1	70	mark	mark	PROPN
ajlm-609	1	71	p.	p.	PROPN
ajlm-609	1	72	nicol	nicol	PROPN
ajlm-609	1	73	faculty	faculty	PROPN
ajlm-609	1	74	of	of	ADP
ajlm-609	1	75	health	health	NOUN
ajlm-609	1	76	sciences	sciences	PROPN
ajlm-609	1	77	,	,	PUNCT
ajlm-609	1	78	university	university	NOUN
ajlm-609	1	79	of	of	ADP
ajlm-609	1	80	cape	cape	PROPN
ajlm-609	1	81	town	town	NOUN
ajlm-609	1	82	,	,	PUNCT
ajlm-609	1	83	cape	cape	NOUN
ajlm-609	1	84	town	town	NOUN
ajlm-609	1	85	,	,	PUNCT
ajlm-609	1	86	south	south	PROPN
ajlm-609	1	87	africa	africa	PROPN
ajlm-609	1	88	lourens	lourens	AUX
ajlm-609	1	89	robberts	robbert	VERB
ajlm-609	1	90	faculty	faculty	NOUN
ajlm-609	1	91	of	of	ADP
ajlm-609	1	92	health	health	NOUN
ajlm-609	1	93	sciences	sciences	PROPN
ajlm-609	1	94	,	,	PUNCT
ajlm-609	1	95	university	university	NOUN
ajlm-609	1	96	of	of	ADP
ajlm-609	1	97	cape	cape	PROPN
ajlm-609	1	98	town	town	NOUN
ajlm-609	1	99	,	,	PUNCT
ajlm-609	1	100	cape	cape	NOUN
ajlm-609	1	101	town	town	NOUN
ajlm-609	1	102	,	,	PUNCT
ajlm-609	1	103	south	south	PROPN
ajlm-609	1	104	africa	africa	PROPN
ajlm-609	1	105	citation	citation	PROPN
ajlm-609	1	106	kalule	kalule	PROPN
ajlm-609	1	107	jb	jb	PROPN
ajlm-609	1	108	,	,	PUNCT
ajlm-609	1	109	keddy	keddy	PROPN
ajlm-609	1	110	kh	kh	PROPN
ajlm-609	1	111	,	,	PUNCT
ajlm-609	1	112	smith	smith	PROPN
ajlm-609	1	113	a	a	PROPN
ajlm-609	1	114	,	,	PUNCT
ajlm-609	1	115	nicol	nicol	PROPN
ajlm-609	1	116	mp	mp	PROPN
ajlm-609	1	117	,	,	PUNCT
ajlm-609	1	118	robberts	robbert	VERB
ajlm-609	1	119	l.	l.	PROPN
ajlm-609	1	120	development	development	PROPN
ajlm-609	1	121	of	of	ADP
ajlm-609	1	122	a	a	DET
ajlm-609	1	123	real	real	ADJ
ajlm-609	1	124	-	-	PUNCT
ajlm-609	1	125	time	time	NOUN
ajlm-609	1	126	pcr	pcr	NOUN
ajlm-609	1	127	assay	assay	NOUN
ajlm-609	1	128	and	and	CCONJ
ajlm-609	1	129	comparison	comparison	NOUN
ajlm-609	1	130	to	to	PART
ajlm-609	1	131	chromagartm	chromagartm	VERB
ajlm-609	1	132	stec	stec	ADJ
ajlm-609	1	133	to	to	AUX
ajlm-609	1	134	screen	screen	VERB
ajlm-609	1	135	for	for	ADP
ajlm-609	1	136	shiga	shiga	ADJ
ajlm-609	1	137	toxin	toxin	NOUN
ajlm-609	1	138	-	-	PUNCT
ajlm-609	1	139	producing	produce	VERB
ajlm-609	1	140	escherichia	escherichia	NOUN
ajlm-609	1	141	coli	coli	NOUN
ajlm-609	1	142	in	in	ADP
ajlm-609	1	143	stool	stool	NOUN
ajlm-609	1	144	,	,	PUNCT
ajlm-609	1	145	cape	cape	NOUN
ajlm-609	1	146	town	town	NOUN
ajlm-609	1	147	,	,	PUNCT
ajlm-609	1	148	south	south	PROPN
ajlm-609	1	149	africa	africa	PROPN
ajlm-609	1	150	.	.	PUNCT
ajlm-609	2	1	afr	afr	PROPN
ajlm-609	2	2	j	j	PROPN
ajlm-609	2	3	lab	lab	PROPN
ajlm-609	2	4	med	me	VERB
ajlm-609	2	5	.	.	PUNCT
ajlm-609	2	6	2017;6(1	2017;6(1	NUM
ajlm-609	2	7	)	)	PUNCT
ajlm-609	2	8	,	,	PUNCT
ajlm-609	2	9	a609	a609	PROPN
ajlm-609	2	10	.	.	PROPN
ajlm-609	3	1	https://doi.org/10.4102/ajlm.v6i1.609	https://doi.org/10.4102/ajlm.v6i1.609	VERB
ajlm-609	3	2	original	original	ADJ
ajlm-609	3	3	research	research	NOUN
ajlm-609	3	4	development	development	NOUN
ajlm-609	3	5	of	of	ADP
ajlm-609	3	6	a	a	DET
ajlm-609	3	7	real	real	ADJ
ajlm-609	3	8	-	-	PUNCT
ajlm-609	3	9	time	time	NOUN
ajlm-609	3	10	pcr	pcr	NOUN
ajlm-609	3	11	assay	assay	NOUN
ajlm-609	3	12	and	and	CCONJ
ajlm-609	3	13	comparison	comparison	NOUN
ajlm-609	3	14	to	to	PART
ajlm-609	3	15	chromagartm	chromagartm	VERB
ajlm-609	3	16	stec	stec	ADJ
ajlm-609	3	17	to	to	AUX
ajlm-609	3	18	screen	screen	VERB
ajlm-609	3	19	for	for	ADP
ajlm-609	3	20	shiga	shiga	ADJ
ajlm-609	3	21	toxin	toxin	NOUN
ajlm-609	3	22	-	-	PUNCT
ajlm-609	3	23	producing	produce	VERB
ajlm-609	3	24	escherichia	escherichia	NOUN
ajlm-609	3	25	coli	coli	NOUN
ajlm-609	3	26	in	in	ADP
ajlm-609	3	27	stool	stool	NOUN
ajlm-609	3	28	,	,	PUNCT
ajlm-609	3	29	cape	cape	NOUN
ajlm-609	3	30	town	town	NOUN
ajlm-609	3	31	,	,	PUNCT
ajlm-609	3	32	south	south	PROPN
ajlm-609	3	33	africa	africa	PROPN
ajlm-609	3	34	john	john	PROPN
ajlm-609	3	35	b.	b.	PROPN
ajlm-609	3	36	kalule	kalule	PROPN
ajlm-609	3	37	,	,	PUNCT
ajlm-609	3	38	karen	karen	PROPN
ajlm-609	3	39	h.	h.	PROPN
ajlm-609	3	40	keddy	keddy	PROPN
ajlm-609	3	41	,	,	PUNCT
ajlm-609	3	42	anthony	anthony	PROPN
ajlm-609	3	43	smith	smith	PROPN
ajlm-609	3	44	,	,	PUNCT
ajlm-609	3	45	mark	mark	PROPN
ajlm-609	3	46	p.	p.	PROPN
ajlm-609	3	47	nicol	nicol	PROPN
ajlm-609	3	48	,	,	PUNCT
ajlm-609	3	49	lourens	lourens	ADP
ajlm-609	3	50	robberts	robbert	NOUN
ajlm-609	3	51	received	receive	VERB
ajlm-609	3	52	:	:	PUNCT
ajlm-609	3	53	28	28	NUM
ajlm-609	3	54	jan	jan	PROPN
ajlm-609	3	55	.	.	PROPN
ajlm-609	3	56	2017	2017	NUM
ajlm-609	3	57	;	;	PUNCT
ajlm-609	3	58	accepted	accept	VERB
ajlm-609	3	59	:	:	PUNCT
ajlm-609	3	60	19	19	NUM
ajlm-609	3	61	sept	sept	PROPN
ajlm-609	3	62	.	.	PROPN
ajlm-609	3	63	2017	2017	NUM
ajlm-609	3	64	;	;	PUNCT
ajlm-609	3	65	published	publish	VERB
ajlm-609	3	66	:	:	PUNCT
ajlm-609	3	67	14	14	NUM
ajlm-609	3	68	dec	dec	PROPN
ajlm-609	3	69	.	.	PROPN
ajlm-609	4	1	2017	2017	NUM
ajlm-609	4	2	copyright	copyright	NOUN
ajlm-609	4	3	:	:	PUNCT
ajlm-609	4	4	©	©	PROPN
ajlm-609	4	5	2017	2017	NUM
ajlm-609	4	6	.	.	PUNCT
ajlm-609	5	1	the	the	DET
ajlm-609	5	2	author(s	author(s	PROPN
ajlm-609	5	3	)	)	PUNCT
ajlm-609	5	4	.	.	PUNCT
ajlm-609	6	1	licensee	licensee	NOUN
ajlm-609	6	2	:	:	PUNCT
ajlm-609	6	3	aosis	aosis	NOUN
ajlm-609	6	4	.	.	PUNCT
ajlm-609	7	1	this	this	PRON
ajlm-609	7	2	is	be	AUX
ajlm-609	7	3	an	an	DET
ajlm-609	7	4	open	open	ADJ
ajlm-609	7	5	access	access	NOUN
ajlm-609	7	6	article	article	NOUN
ajlm-609	7	7	distributed	distribute	VERB
ajlm-609	7	8	under	under	ADP
ajlm-609	7	9	the	the	DET
ajlm-609	7	10	terms	term	NOUN
ajlm-609	7	11	of	of	ADP
ajlm-609	7	12	the	the	DET
ajlm-609	7	13	creative	creative	ADJ
ajlm-609	7	14	commons	common	NOUN
ajlm-609	7	15	attribution	attribution	NOUN
ajlm-609	7	16	license	license	NOUN
ajlm-609	7	17	,	,	PUNCT
ajlm-609	7	18	which	which	PRON
ajlm-609	7	19	permits	permit	VERB
ajlm-609	7	20	unrestricted	unrestricted	ADJ
ajlm-609	7	21	use	use	NOUN
ajlm-609	7	22	,	,	PUNCT
ajlm-609	7	23	distribution	distribution	NOUN
ajlm-609	7	24	,	,	PUNCT
ajlm-609	7	25	and	and	CCONJ
ajlm-609	7	26	reproduction	reproduction	NOUN
ajlm-609	7	27	in	in	ADP
ajlm-609	7	28	any	any	DET
ajlm-609	7	29	medium	medium	NOUN
ajlm-609	7	30	,	,	PUNCT
ajlm-609	7	31	provided	provide	VERB
ajlm-609	7	32	the	the	DET
ajlm-609	7	33	original	original	ADJ
ajlm-609	7	34	work	work	NOUN
ajlm-609	7	35	is	be	AUX
ajlm-609	7	36	properly	properly	ADV
ajlm-609	7	37	cited	cite	VERB
ajlm-609	7	38	.	.	PUNCT
ajlm-609	8	1	abstract	abstract	ADJ
ajlm-609	8	2	introduction	introduction	NOUN
ajlm-609	8	3	:	:	PUNCT
ajlm-609	8	4	shiga	shiga	ADJ
ajlm-609	8	5	toxin	toxin	NOUN
ajlm-609	8	6	-	-	PUNCT
ajlm-609	8	7	producing	produce	VERB
ajlm-609	8	8	escherichia	escherichia	NOUN
ajlm-609	8	9	coli	coli	NOUN
ajlm-609	8	10	(	(	PUNCT
ajlm-609	8	11	stec	stec	ADJ
ajlm-609	8	12	)	)	PUNCT
ajlm-609	8	13	is	be	AUX
ajlm-609	8	14	an	an	DET
ajlm-609	8	15	emerging	emerge	VERB
ajlm-609	8	16	infectious	infectious	ADJ
ajlm-609	8	17	pathogen	pathogen	NOUN
ajlm-609	8	18	which	which	PRON
ajlm-609	8	19	could	could	AUX
ajlm-609	8	20	lead	lead	VERB
ajlm-609	8	21	to	to	ADP
ajlm-609	8	22	haemolytic	haemolytic	ADJ
ajlm-609	8	23	uremic	uremic	ADJ
ajlm-609	8	24	syndrome	syndrome	NOUN
ajlm-609	8	25	.	.	PUNCT
ajlm-609	9	1	even	even	ADV
ajlm-609	9	2	though	though	SCONJ
ajlm-609	9	3	previous	previous	ADJ
ajlm-609	9	4	studies	study	NOUN
ajlm-609	9	5	have	have	AUX
ajlm-609	9	6	compared	compare	VERB
ajlm-609	9	7	the	the	DET
ajlm-609	9	8	performance	performance	NOUN
ajlm-609	9	9	of	of	ADP
ajlm-609	9	10	chromagartmstec	chromagartmstec	NOUN
ajlm-609	9	11	to	to	ADP
ajlm-609	9	12	real	real	ADJ
ajlm-609	9	13	-	-	PUNCT
ajlm-609	9	14	time	time	NOUN
ajlm-609	9	15	polymerase	polymerase	NOUN
ajlm-609	9	16	chain	chain	NOUN
ajlm-609	9	17	reaction	reaction	NOUN
ajlm-609	9	18	(	(	PUNCT
ajlm-609	9	19	pcr	pcr	PROPN
ajlm-609	9	20	)	)	PUNCT
ajlm-609	9	21	in	in	ADP
ajlm-609	9	22	europe	europe	PROPN
ajlm-609	9	23	,	,	PUNCT
ajlm-609	9	24	no	no	DET
ajlm-609	9	25	study	study	NOUN
ajlm-609	9	26	has	have	AUX
ajlm-609	9	27	been	be	AUX
ajlm-609	9	28	done	do	VERB
ajlm-609	9	29	to	to	PART
ajlm-609	9	30	assess	assess	VERB
ajlm-609	9	31	its	its	PRON
ajlm-609	9	32	performance	performance	NOUN
ajlm-609	9	33	on	on	ADP
ajlm-609	9	34	african	african	ADJ
ajlm-609	9	35	isolates	isolate	NOUN
ajlm-609	9	36	.	.	PUNCT
ajlm-609	10	1	objectives	objective	NOUN
ajlm-609	10	2	:	:	PUNCT
ajlm-609	10	3	this	this	DET
ajlm-609	10	4	project	project	NOUN
ajlm-609	10	5	aimed	aim	VERB
ajlm-609	10	6	to	to	PART
ajlm-609	10	7	validate	validate	VERB
ajlm-609	10	8	and	and	CCONJ
ajlm-609	10	9	test	test	VERB
ajlm-609	10	10	an	an	DET
ajlm-609	10	11	in	in	ADP
ajlm-609	10	12	-	-	PUNCT
ajlm-609	10	13	house	house	NOUN
ajlm-609	10	14	-	-	PUNCT
ajlm-609	10	15	developed	develop	VERB
ajlm-609	10	16	duplex	duplex	NOUN
ajlm-609	10	17	real	real	ADJ
ajlm-609	10	18	-	-	PUNCT
ajlm-609	10	19	time	time	NOUN
ajlm-609	10	20	pcr	pcr	NOUN
ajlm-609	10	21	and	and	CCONJ
ajlm-609	10	22	use	use	VERB
ajlm-609	10	23	it	it	PRON
ajlm-609	10	24	as	as	ADP
ajlm-609	10	25	a	a	DET
ajlm-609	10	26	reference	reference	NOUN
ajlm-609	10	27	standard	standard	NOUN
ajlm-609	10	28	to	to	PART
ajlm-609	10	29	determine	determine	VERB
ajlm-609	10	30	the	the	DET
ajlm-609	10	31	performance	performance	NOUN
ajlm-609	10	32	of	of	ADP
ajlm-609	10	33	chromagartmstec	chromagartmstec	ADJ
ajlm-609	10	34	on	on	ADP
ajlm-609	10	35	african	african	ADJ
ajlm-609	10	36	isolates	isolate	NOUN
ajlm-609	10	37	from	from	ADP
ajlm-609	10	38	diarrhoeic	diarrhoeic	ADJ
ajlm-609	10	39	stool	stool	NOUN
ajlm-609	10	40	samples	sample	NOUN
ajlm-609	10	41	.	.	PUNCT
ajlm-609	11	1	methods	method	NOUN
ajlm-609	11	2	:	:	PUNCT
ajlm-609	11	3	this	this	DET
ajlm-609	11	4	study	study	NOUN
ajlm-609	11	5	evaluated	evaluate	VERB
ajlm-609	11	6	stec	stec	ADJ
ajlm-609	11	7	diagnostic	diagnostic	ADJ
ajlm-609	11	8	technology	technology	NOUN
ajlm-609	11	9	on	on	ADP
ajlm-609	11	10	african	african	ADJ
ajlm-609	11	11	isolates	isolate	NOUN
ajlm-609	11	12	.	.	PUNCT
ajlm-609	12	1	an	an	DET
ajlm-609	12	2	in	in	ADP
ajlm-609	12	3	-	-	PUNCT
ajlm-609	12	4	house	house	NOUN
ajlm-609	12	5	-	-	PUNCT
ajlm-609	12	6	developed	develop	VERB
ajlm-609	12	7	duplex	duplex	NOUN
ajlm-609	12	8	real	real	ADJ
ajlm-609	12	9	-	-	PUNCT
ajlm-609	12	10	time	time	NOUN
ajlm-609	12	11	pcr	pcr	NOUN
ajlm-609	12	12	assay	assay	NOUN
ajlm-609	12	13	for	for	ADP
ajlm-609	12	14	detection	detection	NOUN
ajlm-609	12	15	of	of	ADP
ajlm-609	12	16	stx1	stx1	NOUN
ajlm-609	12	17	and	and	CCONJ
ajlm-609	12	18	stx2	stx2	PROPN
ajlm-609	12	19	was	be	AUX
ajlm-609	12	20	validated	validate	VERB
ajlm-609	12	21	and	and	CCONJ
ajlm-609	12	22	tested	test	VERB
ajlm-609	12	23	on	on	ADP
ajlm-609	12	24	diarrhoeic	diarrhoeic	VERB
ajlm-609	12	25	stool	stool	NOUN
ajlm-609	12	26	samples	sample	NOUN
ajlm-609	12	27	and	and	CCONJ
ajlm-609	12	28	then	then	ADV
ajlm-609	12	29	used	use	VERB
ajlm-609	12	30	as	as	ADP
ajlm-609	12	31	a	a	DET
ajlm-609	12	32	reference	reference	NOUN
ajlm-609	12	33	standard	standard	NOUN
ajlm-609	12	34	to	to	PART
ajlm-609	12	35	assess	assess	VERB
ajlm-609	12	36	the	the	DET
ajlm-609	12	37	performance	performance	NOUN
ajlm-609	12	38	of	of	ADP
ajlm-609	12	39	chromagartmstec	chromagartmstec	ADJ
ajlm-609	12	40	.	.	PUNCT
ajlm-609	13	1	real	real	ADJ
ajlm-609	13	2	-	-	PUNCT
ajlm-609	13	3	time	time	NOUN
ajlm-609	13	4	pcr	pcr	NOUN
ajlm-609	13	5	was	be	AUX
ajlm-609	13	6	used	use	VERB
ajlm-609	13	7	to	to	PART
ajlm-609	13	8	screen	screen	VERB
ajlm-609	13	9	for	for	ADP
ajlm-609	13	10	stx	stx	NOUN
ajlm-609	13	11	in	in	ADP
ajlm-609	13	12	tryptic	tryptic	ADJ
ajlm-609	13	13	soy	soy	NOUN
ajlm-609	13	14	broth	broth	NOUN
ajlm-609	13	15	and	and	CCONJ
ajlm-609	13	16	the	the	DET
ajlm-609	13	17	suspected	suspect	VERB
ajlm-609	13	18	stec	stec	ADJ
ajlm-609	13	19	isolates	isolate	NOUN
ajlm-609	13	20	,	,	PUNCT
ajlm-609	13	21	while	while	SCONJ
ajlm-609	13	22	conventional	conventional	ADJ
ajlm-609	13	23	pcr	pcr	NOUN
ajlm-609	13	24	was	be	AUX
ajlm-609	13	25	used	use	VERB
ajlm-609	13	26	to	to	PART
ajlm-609	13	27	detect	detect	VERB
ajlm-609	13	28	the	the	DET
ajlm-609	13	29	other	other	ADJ
ajlm-609	13	30	virulence	virulence	NOUN
ajlm-609	13	31	genes	gene	NOUN
ajlm-609	13	32	possessed	possess	VERB
ajlm-609	13	33	by	by	ADP
ajlm-609	13	34	the	the	DET
ajlm-609	13	35	isolates	isolate	NOUN
ajlm-609	13	36	.	.	PUNCT
ajlm-609	14	1	results	result	NOUN
ajlm-609	14	2	:	:	PUNCT
ajlm-609	14	3	the	the	DET
ajlm-609	14	4	real	real	ADJ
ajlm-609	14	5	-	-	PUNCT
ajlm-609	14	6	time	time	NOUN
ajlm-609	14	7	pcr	pcr	NOUN
ajlm-609	14	8	limit	limit	NOUN
ajlm-609	14	9	of	of	ADP
ajlm-609	14	10	detection	detection	NOUN
ajlm-609	14	11	was	be	AUX
ajlm-609	14	12	5.3	5.3	NUM
ajlm-609	14	13	target	target	NOUN
ajlm-609	14	14	copies	copy	NOUN
ajlm-609	14	15	/	/	SYM
ajlm-609	14	16	μl	μl	NUM
ajlm-609	14	17	of	of	ADP
ajlm-609	14	18	broth	broth	NOUN
ajlm-609	14	19	.	.	PUNCT
ajlm-609	15	1	the	the	DET
ajlm-609	15	2	mean	mean	ADJ
ajlm-609	15	3	melting	melt	VERB
ajlm-609	15	4	temperature	temperature	NOUN
ajlm-609	15	5	on	on	ADP
ajlm-609	15	6	melt	melt	NOUN
ajlm-609	15	7	-	-	PUNCT
ajlm-609	15	8	curve	curve	NOUN
ajlm-609	15	9	analysis	analysis	NOUN
ajlm-609	15	10	for	for	ADP
ajlm-609	15	11	detection	detection	NOUN
ajlm-609	15	12	of	of	ADP
ajlm-609	15	13	stx1	stx1	NOUN
ajlm-609	15	14	was	be	AUX
ajlm-609	15	15	58.2	58.2	NUM
ajlm-609	15	16	°	°	ADP
ajlm-609	15	17	c	c	NOUN
ajlm-609	15	18	and	and	CCONJ
ajlm-609	15	19	for	for	ADP
ajlm-609	15	20	stx2	stx2	NOUN
ajlm-609	15	21	was	be	AUX
ajlm-609	15	22	65.3	65.3	NUM
ajlm-609	15	23	°	°	NOUN
ajlm-609	15	24	c	c	NOUN
ajlm-609	15	25	.	.	PUNCT
ajlm-609	16	1	of	of	ADP
ajlm-609	16	2	226	226	NUM
ajlm-609	16	3	specimens	specimen	NOUN
ajlm-609	16	4	screened	screen	VERB
ajlm-609	16	5	,	,	PUNCT
ajlm-609	16	6	real	real	ADJ
ajlm-609	16	7	-	-	PUNCT
ajlm-609	16	8	time	time	NOUN
ajlm-609	16	9	pcr	pcr	PROPN
ajlm-609	16	10	detected	detect	VERB
ajlm-609	16	11	stx	stx	NOUN
ajlm-609	16	12	in	in	ADP
ajlm-609	16	13	14	14	NUM
ajlm-609	16	14	specimens	specimen	NOUN
ajlm-609	16	15	(	(	PUNCT
ajlm-609	16	16	6.2	6.2	NUM
ajlm-609	16	17	%	%	NOUN
ajlm-609	16	18	,	,	PUNCT
ajlm-609	16	19	95	95	NUM
ajlm-609	16	20	%	%	NOUN
ajlm-609	16	21	confidence	confidence	NOUN
ajlm-609	16	22	interval	interval	NOUN
ajlm-609	16	23	=	=	NOUN
ajlm-609	16	24	3.43	3.43	NUM
ajlm-609	16	25	%	%	NOUN
ajlm-609	16	26	–	–	PUNCT
ajlm-609	16	27	10.18	10.18	NUM
ajlm-609	16	28	%	%	NOUN
ajlm-609	16	29	)	)	PUNCT
ajlm-609	16	30	.	.	PUNCT
ajlm-609	17	1	the	the	DET
ajlm-609	17	2	sensitivity	sensitivity	NOUN
ajlm-609	17	3	,	,	PUNCT
ajlm-609	17	4	specificity	specificity	NOUN
ajlm-609	17	5	,	,	PUNCT
ajlm-609	17	6	negative	negative	ADJ
ajlm-609	17	7	predictive	predictive	ADJ
ajlm-609	17	8	value	value	NOUN
ajlm-609	17	9	and	and	CCONJ
ajlm-609	17	10	positive	positive	ADJ
ajlm-609	17	11	predictive	predictive	ADJ
ajlm-609	17	12	value	value	NOUN
ajlm-609	17	13	of	of	ADP
ajlm-609	17	14	the	the	DET
ajlm-609	17	15	chromagartmstec	chromagartmstec	ADJ
ajlm-609	17	16	were	be	AUX
ajlm-609	17	17	33.3	33.3	NUM
ajlm-609	17	18	%	%	NOUN
ajlm-609	17	19	,	,	PUNCT
ajlm-609	17	20	77.4	77.4	NUM
ajlm-609	17	21	%	%	NOUN
ajlm-609	17	22	,	,	PUNCT
ajlm-609	17	23	95.3	95.3	NUM
ajlm-609	17	24	%	%	NOUN
ajlm-609	17	25	and	and	CCONJ
ajlm-609	17	26	11.3	11.3	NUM
ajlm-609	17	27	%	%	NOUN
ajlm-609	17	28	.	.	PUNCT
ajlm-609	18	1	conclusions	conclusion	NOUN
ajlm-609	18	2	:	:	PUNCT
ajlm-609	18	3	the	the	DET
ajlm-609	18	4	in	in	ADP
ajlm-609	18	5	-	-	PUNCT
ajlm-609	18	6	house	house	NOUN
ajlm-609	18	7	developed	develop	VERB
ajlm-609	18	8	real	real	ADJ
ajlm-609	18	9	-	-	PUNCT
ajlm-609	18	10	time	time	NOUN
ajlm-609	18	11	pcr	pcr	PROPN
ajlm-609	18	12	assay	assay	NOUN
ajlm-609	18	13	is	be	AUX
ajlm-609	18	14	a	a	DET
ajlm-609	18	15	sensitive	sensitive	ADJ
ajlm-609	18	16	and	and	CCONJ
ajlm-609	18	17	specific	specific	ADJ
ajlm-609	18	18	option	option	NOUN
ajlm-609	18	19	for	for	ADP
ajlm-609	18	20	laboratory	laboratory	NOUN
ajlm-609	18	21	detection	detection	NOUN
ajlm-609	18	22	of	of	ADP
ajlm-609	18	23	stec	stec	ADJ
ajlm-609	18	24	as	as	SCONJ
ajlm-609	18	25	compared	compare	VERB
ajlm-609	18	26	to	to	PART
ajlm-609	18	27	chromagartmstec	chromagartmstec	VERB
ajlm-609	18	28	in	in	ADP
ajlm-609	18	29	this	this	DET
ajlm-609	18	30	setting	setting	NOUN
ajlm-609	18	31	.	.	PUNCT
ajlm-609	19	1	introduction	introduction	NOUN
ajlm-609	19	2	globally	globally	ADV
ajlm-609	19	3	,	,	PUNCT
ajlm-609	19	4	foodand	foodand	ADJ
ajlm-609	19	5	water	water	NOUN
ajlm-609	19	6	-	-	PUNCT
ajlm-609	19	7	borne	bear	VERB
ajlm-609	19	8	outbreaks	outbreak	NOUN
ajlm-609	19	9	of	of	ADP
ajlm-609	19	10	both	both	DET
ajlm-609	19	11	o157	o157	PROPN
ajlm-609	19	12	and	and	CCONJ
ajlm-609	19	13	non	non	ADJ
ajlm-609	19	14	-	-	ADJ
ajlm-609	19	15	o157	o157	ADJ
ajlm-609	19	16	shiga	shiga	ADJ
ajlm-609	19	17	toxin	toxin	NOUN
ajlm-609	19	18	-	-	PUNCT
ajlm-609	19	19	producing	produce	VERB
ajlm-609	19	20	escherichia	escherichia	NOUN
ajlm-609	19	21	coli	coli	NOUN
ajlm-609	19	22	(	(	PUNCT
ajlm-609	19	23	stec	stec	ADJ
ajlm-609	19	24	)	)	PUNCT
ajlm-609	19	25	have	have	AUX
ajlm-609	19	26	been	be	AUX
ajlm-609	19	27	successfully	successfully	ADV
ajlm-609	19	28	detected	detect	VERB
ajlm-609	19	29	due	due	ADP
ajlm-609	19	30	to	to	ADP
ajlm-609	19	31	the	the	DET
ajlm-609	19	32	availability	availability	NOUN
ajlm-609	19	33	of	of	ADP
ajlm-609	19	34	good	good	ADJ
ajlm-609	19	35	baseline	baseline	PROPN
ajlm-609	19	36	data	datum	NOUN
ajlm-609	19	37	and	and	CCONJ
ajlm-609	19	38	effective	effective	ADJ
ajlm-609	19	39	active	active	ADJ
ajlm-609	19	40	laboratory	laboratory	NOUN
ajlm-609	19	41	-	-	PUNCT
ajlm-609	19	42	based	base	VERB
ajlm-609	19	43	surveillance	surveillance	NOUN
ajlm-609	19	44	systems.1,2,3,4	systems.1,2,3,4	VERB
ajlm-609	19	45	early	early	ADJ
ajlm-609	19	46	detection	detection	NOUN
ajlm-609	19	47	of	of	ADP
ajlm-609	19	48	outbreaks	outbreak	NOUN
ajlm-609	19	49	is	be	AUX
ajlm-609	19	50	important	important	ADJ
ajlm-609	19	51	to	to	PART
ajlm-609	19	52	minimise	minimise	VERB
ajlm-609	19	53	morbidity	morbidity	NOUN
ajlm-609	19	54	,	,	PUNCT
ajlm-609	19	55	mortality	mortality	NOUN
ajlm-609	19	56	and	and	CCONJ
ajlm-609	19	57	associated	associate	VERB
ajlm-609	19	58	economic	economic	ADJ
ajlm-609	19	59	losses.5	losses.5	PROPN
ajlm-609	19	60	there	there	PRON
ajlm-609	19	61	is	be	VERB
ajlm-609	19	62	a	a	DET
ajlm-609	19	63	lack	lack	NOUN
ajlm-609	19	64	of	of	ADP
ajlm-609	19	65	good	good	ADJ
ajlm-609	19	66	baseline	baseline	PROPN
ajlm-609	19	67	data	datum	NOUN
ajlm-609	19	68	on	on	ADP
ajlm-609	19	69	stec	stec	ADJ
ajlm-609	19	70	in	in	ADP
ajlm-609	19	71	africa	africa	PROPN
ajlm-609	19	72	,	,	PUNCT
ajlm-609	19	73	which	which	PRON
ajlm-609	19	74	can	can	AUX
ajlm-609	19	75	be	be	AUX
ajlm-609	19	76	attributed	attribute	VERB
ajlm-609	19	77	to	to	ADP
ajlm-609	19	78	a	a	DET
ajlm-609	19	79	lack	lack	NOUN
ajlm-609	19	80	of	of	ADP
ajlm-609	19	81	laboratory	laboratory	NOUN
ajlm-609	19	82	resources	resource	NOUN
ajlm-609	19	83	and	and	CCONJ
ajlm-609	19	84	the	the	DET
ajlm-609	19	85	surveillance	surveillance	NOUN
ajlm-609	19	86	strategy	strategy	NOUN
ajlm-609	19	87	employed	employ	VERB
ajlm-609	19	88	.	.	PUNCT
ajlm-609	20	1	stec	stec	PROPN
ajlm-609	20	2	has	have	AUX
ajlm-609	20	3	been	be	AUX
ajlm-609	20	4	implicated	implicate	VERB
ajlm-609	20	5	in	in	ADP
ajlm-609	20	6	outbreaks	outbreak	NOUN
ajlm-609	20	7	of	of	ADP
ajlm-609	20	8	bloody	bloody	ADJ
ajlm-609	20	9	diarrhoea	diarrhoea	NOUN
ajlm-609	20	10	in	in	ADP
ajlm-609	20	11	sub	sub	ADJ
ajlm-609	20	12	-	-	ADJ
ajlm-609	20	13	saharan	saharan	ADJ
ajlm-609	20	14	countries;6,7,8	countries;6,7,8	NOUN
ajlm-609	20	15	however	however	ADV
ajlm-609	20	16	,	,	PUNCT
ajlm-609	20	17	these	these	PRON
ajlm-609	20	18	have	have	AUX
ajlm-609	20	19	been	be	AUX
ajlm-609	20	20	difficult	difficult	ADJ
ajlm-609	20	21	to	to	PART
ajlm-609	20	22	track	track	VERB
ajlm-609	20	23	and	and	CCONJ
ajlm-609	20	24	manage	manage	VERB
ajlm-609	20	25	due	due	ADJ
ajlm-609	20	26	to	to	ADP
ajlm-609	20	27	laboratory	laboratory	NOUN
ajlm-609	20	28	weakness.9,10	weakness.9,10	ADV
ajlm-609	20	29	furthermore	furthermore	ADV
ajlm-609	20	30	,	,	PUNCT
ajlm-609	20	31	typical	typical	ADJ
ajlm-609	20	32	haemolytic	haemolytic	ADJ
ajlm-609	20	33	uremic	uremic	ADJ
ajlm-609	20	34	syndrome	syndrome	NOUN
ajlm-609	20	35	,	,	PUNCT
ajlm-609	20	36	which	which	PRON
ajlm-609	20	37	is	be	AUX
ajlm-609	20	38	overwhelmingly	overwhelmingly	ADV
ajlm-609	20	39	caused	cause	VERB
ajlm-609	20	40	by	by	ADP
ajlm-609	20	41	stec	stec	ADJ
ajlm-609	20	42	,	,	PUNCT
ajlm-609	20	43	was	be	AUX
ajlm-609	20	44	reported	report	VERB
ajlm-609	20	45	as	as	SCONJ
ajlm-609	20	46	the	the	DET
ajlm-609	20	47	leading	lead	VERB
ajlm-609	20	48	contributor	contributor	NOUN
ajlm-609	20	49	to	to	PART
ajlm-609	20	50	acute	acute	VERB
ajlm-609	20	51	renal	renal	ADJ
ajlm-609	20	52	failure	failure	NOUN
ajlm-609	20	53	in	in	ADP
ajlm-609	20	54	paediatric	paediatric	ADJ
ajlm-609	20	55	patients	patient	NOUN
ajlm-609	20	56	at	at	ADP
ajlm-609	20	57	a	a	DET
ajlm-609	20	58	south	south	ADJ
ajlm-609	20	59	african	african	ADJ
ajlm-609	20	60	academic	academic	ADJ
ajlm-609	20	61	hospital.11	hospital.11	NOUN
ajlm-609	20	62	even	even	ADV
ajlm-609	20	63	though	though	SCONJ
ajlm-609	20	64	several	several	ADJ
ajlm-609	20	65	studies	study	NOUN
ajlm-609	20	66	have	have	AUX
ajlm-609	20	67	evaluated	evaluate	VERB
ajlm-609	20	68	the	the	DET
ajlm-609	20	69	performance	performance	NOUN
ajlm-609	20	70	of	of	ADP
ajlm-609	20	71	chromagartmstec	chromagartmstec	NOUN
ajlm-609	20	72	by	by	ADP
ajlm-609	20	73	comparison	comparison	NOUN
ajlm-609	20	74	to	to	ADP
ajlm-609	20	75	molecular	molecular	ADJ
ajlm-609	20	76	and	and	CCONJ
ajlm-609	20	77	antigen	antigen	ADJ
ajlm-609	20	78	detection	detection	NOUN
ajlm-609	20	79	methods	method	NOUN
ajlm-609	20	80	in	in	ADP
ajlm-609	20	81	developed	developed	ADJ
ajlm-609	20	82	countries,12,13	countries,12,13	NOUN
ajlm-609	20	83	no	no	DET
ajlm-609	20	84	study	study	NOUN
ajlm-609	20	85	has	have	AUX
ajlm-609	20	86	so	so	ADV
ajlm-609	20	87	far	far	ADV
ajlm-609	20	88	evaluated	evaluate	VERB
ajlm-609	20	89	its	its	PRON
ajlm-609	20	90	performance	performance	NOUN
ajlm-609	20	91	in	in	ADP
ajlm-609	20	92	africa	africa	PROPN
ajlm-609	20	93	.	.	PUNCT
ajlm-609	21	1	this	this	PRON
ajlm-609	21	2	is	be	AUX
ajlm-609	21	3	necessary	necessary	ADJ
ajlm-609	21	4	,	,	PUNCT
ajlm-609	21	5	especially	especially	ADV
ajlm-609	21	6	given	give	VERB
ajlm-609	21	7	that	that	SCONJ
ajlm-609	21	8	there	there	PRON
ajlm-609	21	9	are	be	VERB
ajlm-609	21	10	geographical	geographical	ADJ
ajlm-609	21	11	differences	difference	NOUN
ajlm-609	21	12	in	in	ADP
ajlm-609	21	13	characteristics	characteristic	NOUN
ajlm-609	21	14	of	of	ADP
ajlm-609	21	15	stec	stec	ADJ
ajlm-609	21	16	that	that	PRON
ajlm-609	21	17	are	be	AUX
ajlm-609	21	18	dependent	dependent	ADJ
ajlm-609	21	19	on	on	ADP
ajlm-609	21	20	the	the	DET
ajlm-609	21	21	index	index	NOUN
ajlm-609	21	22	of	of	ADP
ajlm-609	21	23	suspicion	suspicion	NOUN
ajlm-609	21	24	for	for	ADP
ajlm-609	21	25	the	the	DET
ajlm-609	21	26	different	different	ADJ
ajlm-609	21	27	stec	stec	ADJ
ajlm-609	21	28	serotypes	serotype	NOUN
ajlm-609	21	29	and	and	CCONJ
ajlm-609	21	30	on	on	ADP
ajlm-609	21	31	the	the	DET
ajlm-609	21	32	availability	availability	NOUN
ajlm-609	21	33	of	of	ADP
ajlm-609	21	34	suitable	suitable	ADJ
ajlm-609	21	35	laboratory	laboratory	NOUN
ajlm-609	21	36	methods	method	NOUN
ajlm-609	21	37	to	to	PART
ajlm-609	21	38	detect	detect	VERB
ajlm-609	21	39	them.14	them.14	NOUN
ajlm-609	21	40	in	in	ADP
ajlm-609	21	41	many	many	ADJ
ajlm-609	21	42	south	south	ADJ
ajlm-609	21	43	african	african	ADJ
ajlm-609	21	44	(	(	PUNCT
ajlm-609	21	45	and	and	CCONJ
ajlm-609	21	46	african	african	ADJ
ajlm-609	21	47	)	)	PUNCT
ajlm-609	21	48	laboratories	laboratory	NOUN
ajlm-609	21	49	,	,	PUNCT
ajlm-609	21	50	stool	stool	NOUN
ajlm-609	21	51	specimens	specimen	NOUN
ajlm-609	21	52	are	be	AUX
ajlm-609	21	53	not	not	PART
ajlm-609	21	54	routinely	routinely	ADV
ajlm-609	21	55	tested	test	VERB
ajlm-609	21	56	for	for	ADP
ajlm-609	21	57	stec	stec	ADJ
ajlm-609	21	58	,	,	PUNCT
ajlm-609	21	59	although	although	SCONJ
ajlm-609	21	60	physicians	physician	NOUN
ajlm-609	21	61	may	may	AUX
ajlm-609	21	62	request	request	VERB
ajlm-609	21	63	tests	test	NOUN
ajlm-609	21	64	specific	specific	ADJ
ajlm-609	21	65	for	for	ADP
ajlm-609	21	66	e.	e.	PROPN
ajlm-609	21	67	coli	coli	PROPN
ajlm-609	21	68	serotype	serotype	NOUN
ajlm-609	21	69	o157	o157	PROPN
ajlm-609	21	70	:	:	PUNCT
ajlm-609	21	71	h7	h7	PROPN
ajlm-609	21	72	,	,	PUNCT
ajlm-609	21	73	if	if	SCONJ
ajlm-609	21	74	it	it	PRON
ajlm-609	21	75	is	be	AUX
ajlm-609	21	76	clinically	clinically	ADV
ajlm-609	21	77	suspected	suspect	VERB
ajlm-609	21	78	.	.	PUNCT
ajlm-609	22	1	testing	testing	NOUN
ajlm-609	22	2	is	be	AUX
ajlm-609	22	3	based	base	VERB
ajlm-609	22	4	on	on	ADP
ajlm-609	22	5	the	the	DET
ajlm-609	22	6	non	non	ADJ
ajlm-609	22	7	-	-	ADJ
ajlm-609	22	8	sorbitol	sorbitol	ADJ
ajlm-609	22	9	fermenting	fermenting	NOUN
ajlm-609	22	10	property	property	NOUN
ajlm-609	22	11	using	use	VERB
ajlm-609	22	12	sorbitol	sorbitol	NOUN
ajlm-609	22	13	macconkey	macconkey	NOUN
ajlm-609	22	14	and	and	CCONJ
ajlm-609	22	15	only	only	ADV
ajlm-609	22	16	on	on	ADP
ajlm-609	22	17	request	request	NOUN
ajlm-609	22	18	by	by	ADP
ajlm-609	22	19	a	a	DET
ajlm-609	22	20	physician	physician	NOUN
ajlm-609	22	21	.	.	PUNCT
ajlm-609	23	1	this	this	DET
ajlm-609	23	2	practice	practice	NOUN
ajlm-609	23	3	is	be	AUX
ajlm-609	23	4	of	of	ADP
ajlm-609	23	5	concern	concern	NOUN
ajlm-609	23	6	,	,	PUNCT
ajlm-609	23	7	is	be	AUX
ajlm-609	23	8	misleading	mislead	VERB
ajlm-609	23	9	and	and	CCONJ
ajlm-609	23	10	underestimates	underestimate	VERB
ajlm-609	23	11	the	the	DET
ajlm-609	23	12	real	real	ADJ
ajlm-609	23	13	magnitude	magnitude	NOUN
ajlm-609	23	14	of	of	ADP
ajlm-609	23	15	stec	stec	ADJ
ajlm-609	23	16	,	,	PUNCT
ajlm-609	23	17	since	since	SCONJ
ajlm-609	23	18	not	not	PART
ajlm-609	23	19	all	all	DET
ajlm-609	23	20	serotype	serotype	ADJ
ajlm-609	23	21	o157	o157	PROPN
ajlm-609	23	22	strains	strain	NOUN
ajlm-609	23	23	are	be	AUX
ajlm-609	23	24	non	non	ADJ
ajlm-609	23	25	-	-	ADJ
ajlm-609	23	26	sorbitol	sorbitol	ADJ
ajlm-609	23	27	fermenting	fermenting	NOUN
ajlm-609	23	28	(	(	PUNCT
ajlm-609	23	29	o157	o157	PROPN
ajlm-609	23	30	:	:	PUNCT
ajlm-609	23	31	nm	nm	NOUN
ajlm-609	23	32	)	)	PUNCT
ajlm-609	23	33	,	,	PUNCT
ajlm-609	23	34	and	and	CCONJ
ajlm-609	23	35	over	over	ADP
ajlm-609	23	36	470	470	NUM
ajlm-609	23	37	non	non	ADJ
ajlm-609	23	38	-	-	ADJ
ajlm-609	23	39	o157	o157	ADJ
ajlm-609	23	40	serotypes	serotype	NOUN
ajlm-609	23	41	have	have	AUX
ajlm-609	23	42	been	be	AUX
ajlm-609	23	43	attributed	attribute	VERB
ajlm-609	23	44	to	to	ADP
ajlm-609	23	45	clinical	clinical	ADJ
ajlm-609	23	46	disease.15	disease.15	NOUN
ajlm-609	23	47	laboratory	laboratory	NOUN
ajlm-609	23	48	capacity	capacity	NOUN
ajlm-609	23	49	for	for	ADP
ajlm-609	23	50	molecular	molecular	ADJ
ajlm-609	23	51	detection	detection	NOUN
ajlm-609	23	52	is	be	AUX
ajlm-609	23	53	increasingly	increasingly	ADV
ajlm-609	23	54	available	available	ADJ
ajlm-609	23	55	in	in	ADP
ajlm-609	23	56	african	african	ADJ
ajlm-609	23	57	countries	country	NOUN
ajlm-609	23	58	and	and	CCONJ
ajlm-609	23	59	may	may	AUX
ajlm-609	23	60	,	,	PUNCT
ajlm-609	23	61	in	in	ADP
ajlm-609	23	62	some	some	DET
ajlm-609	23	63	cases	case	NOUN
ajlm-609	23	64	,	,	PUNCT
ajlm-609	23	65	be	be	AUX
ajlm-609	23	66	simpler	simple	ADJ
ajlm-609	23	67	than	than	ADP
ajlm-609	23	68	culture	culture	NOUN
ajlm-609	23	69	-	-	PUNCT
ajlm-609	23	70	based	base	VERB
ajlm-609	23	71	detection	detection	NOUN
ajlm-609	23	72	.	.	PUNCT
ajlm-609	24	1	this	this	DET
ajlm-609	24	2	project	project	NOUN
ajlm-609	24	3	,	,	PUNCT
ajlm-609	24	4	therefore	therefore	ADV
ajlm-609	24	5	,	,	PUNCT
ajlm-609	24	6	aimed	aim	VERB
ajlm-609	24	7	to	to	PART
ajlm-609	24	8	validate	validate	VERB
ajlm-609	24	9	and	and	CCONJ
ajlm-609	24	10	test	test	VERB
ajlm-609	24	11	diarrhoeic	diarrhoeic	VERB
ajlm-609	24	12	stool	stool	NOUN
ajlm-609	24	13	samples	sample	NOUN
ajlm-609	24	14	by	by	ADP
ajlm-609	24	15	using	use	VERB
ajlm-609	24	16	an	an	DET
ajlm-609	24	17	in	in	ADP
ajlm-609	24	18	-	-	PUNCT
ajlm-609	24	19	house	house	NOUN
ajlm-609	24	20	developed	develop	VERB
ajlm-609	24	21	duplex	duplex	NOUN
ajlm-609	24	22	real	real	ADJ
ajlm-609	24	23	-	-	PUNCT
ajlm-609	24	24	time	time	NOUN
ajlm-609	24	25	polymerase	polymerase	NOUN
ajlm-609	24	26	chain	chain	NOUN
ajlm-609	24	27	reaction	reaction	NOUN
ajlm-609	24	28	(	(	PUNCT
ajlm-609	24	29	pcr	pcr	PROPN
ajlm-609	24	30	)	)	PUNCT
ajlm-609	24	31	and	and	CCONJ
ajlm-609	24	32	use	use	VERB
ajlm-609	24	33	it	it	PRON
ajlm-609	24	34	as	as	ADP
ajlm-609	24	35	a	a	DET
ajlm-609	24	36	reference	reference	NOUN
ajlm-609	24	37	standard	standard	NOUN
ajlm-609	24	38	to	to	PART
ajlm-609	24	39	determine	determine	VERB
ajlm-609	24	40	the	the	DET
ajlm-609	24	41	performance	performance	NOUN
ajlm-609	24	42	of	of	ADP
ajlm-609	24	43	chromagartmstec	chromagartmstec	ADJ
ajlm-609	24	44	on	on	ADP
ajlm-609	24	45	african	african	ADJ
ajlm-609	24	46	isolates	isolate	NOUN
ajlm-609	24	47	.	.	PUNCT
ajlm-609	25	1	the	the	DET
ajlm-609	25	2	duplex	duplex	NOUN
ajlm-609	25	3	assay	assay	NOUN
ajlm-609	25	4	was	be	AUX
ajlm-609	25	5	used	use	VERB
ajlm-609	25	6	to	to	PART
ajlm-609	25	7	screen	screen	VERB
ajlm-609	25	8	tryptic	tryptic	ADJ
ajlm-609	25	9	soy	soy	NOUN
ajlm-609	25	10	broth	broth	NOUN
ajlm-609	25	11	(	(	PUNCT
ajlm-609	25	12	tsb	tsb	PROPN
ajlm-609	25	13	)	)	PUNCT
ajlm-609	25	14	for	for	ADP
ajlm-609	25	15	stx	stx	NOUN
ajlm-609	25	16	following	follow	VERB
ajlm-609	25	17	overnight	overnight	ADJ
ajlm-609	25	18	stool	stool	NOUN
ajlm-609	25	19	enrichment	enrichment	NOUN
ajlm-609	25	20	,	,	PUNCT
ajlm-609	25	21	and	and	CCONJ
ajlm-609	25	22	conventional	conventional	ADJ
ajlm-609	25	23	pcr	pcr	NOUN
ajlm-609	25	24	was	be	AUX
ajlm-609	25	25	used	use	VERB
ajlm-609	25	26	to	to	PART
ajlm-609	25	27	screen	screen	VERB
ajlm-609	25	28	for	for	ADP
ajlm-609	25	29	the	the	DET
ajlm-609	25	30	other	other	ADJ
ajlm-609	25	31	diarrhoeagenic	diarrhoeagenic	ADJ
ajlm-609	25	32	e.	e.	PROPN
ajlm-609	25	33	coli	coli	NOUN
ajlm-609	25	34	virulence	virulence	NOUN
ajlm-609	25	35	genes	gene	NOUN
ajlm-609	25	36	.	.	PUNCT
ajlm-609	26	1	diarrhoeagenic	diarrhoeagenic	PROPN
ajlm-609	26	2	e.	e.	PROPN
ajlm-609	26	3	coli	coli	PROPN
ajlm-609	26	4	were	be	AUX
ajlm-609	26	5	serotyped	serotype	VERB
ajlm-609	26	6	,	,	PUNCT
ajlm-609	26	7	and	and	CCONJ
ajlm-609	26	8	stx	stx	ADJ
ajlm-609	26	9	-	-	ADJ
ajlm-609	26	10	positive	positive	ADJ
ajlm-609	26	11	isolates	isolate	NOUN
ajlm-609	26	12	were	be	AUX
ajlm-609	26	13	tested	test	VERB
ajlm-609	26	14	for	for	ADP
ajlm-609	26	15	shiga	shiga	ADJ
ajlm-609	26	16	toxin	toxin	NOUN
ajlm-609	26	17	production	production	NOUN
ajlm-609	26	18	using	use	VERB
ajlm-609	26	19	immunochromatography	immunochromatography	NOUN
ajlm-609	26	20	.	.	PUNCT
ajlm-609	27	1	methods	method	NOUN
ajlm-609	27	2	ethical	ethical	ADJ
ajlm-609	27	3	approval	approval	NOUN
ajlm-609	27	4	this	this	DET
ajlm-609	27	5	study	study	NOUN
ajlm-609	27	6	was	be	AUX
ajlm-609	27	7	approved	approve	VERB
ajlm-609	27	8	by	by	ADP
ajlm-609	27	9	the	the	DET
ajlm-609	27	10	ethics	ethic	NOUN
ajlm-609	27	11	and	and	CCONJ
ajlm-609	27	12	research	research	NOUN
ajlm-609	27	13	committee	committee	NOUN
ajlm-609	27	14	of	of	ADP
ajlm-609	27	15	the	the	DET
ajlm-609	27	16	faculty	faculty	NOUN
ajlm-609	27	17	of	of	ADP
ajlm-609	27	18	health	health	NOUN
ajlm-609	27	19	sciences	science	NOUN
ajlm-609	27	20	at	at	ADP
ajlm-609	27	21	the	the	DET
ajlm-609	27	22	university	university	PROPN
ajlm-609	27	23	of	of	ADP
ajlm-609	27	24	cape	cape	NOUN
ajlm-609	27	25	town	town	NOUN
ajlm-609	27	26	(	(	PUNCT
ajlm-609	27	27	hrec	hrec	PROPN
ajlm-609	27	28	ref	ref	VERB
ajlm-609	27	29	:	:	PUNCT
ajlm-609	27	30	014/2015	014/2015	NUM
ajlm-609	27	31	)	)	PUNCT
ajlm-609	27	32	.	.	PUNCT
ajlm-609	28	1	study	study	NOUN
ajlm-609	28	2	design	design	NOUN
ajlm-609	28	3	this	this	DET
ajlm-609	28	4	study	study	NOUN
ajlm-609	28	5	validated	validate	VERB
ajlm-609	28	6	an	an	DET
ajlm-609	28	7	in	in	ADP
ajlm-609	28	8	-	-	PUNCT
ajlm-609	28	9	house	house	NOUN
ajlm-609	28	10	-	-	PUNCT
ajlm-609	28	11	developed	develop	VERB
ajlm-609	28	12	duplex	duplex	NOUN
ajlm-609	28	13	real	real	ADJ
ajlm-609	28	14	-	-	PUNCT
ajlm-609	28	15	time	time	NOUN
ajlm-609	28	16	pcr	pcr	NOUN
ajlm-609	28	17	assay	assay	NOUN
ajlm-609	28	18	for	for	ADP
ajlm-609	28	19	detection	detection	NOUN
ajlm-609	28	20	of	of	ADP
ajlm-609	28	21	stx1	stx1	NOUN
ajlm-609	28	22	and	and	CCONJ
ajlm-609	28	23	stx2	stx2	PROPN
ajlm-609	28	24	.	.	PUNCT
ajlm-609	29	1	the	the	DET
ajlm-609	29	2	assay	assay	NOUN
ajlm-609	29	3	was	be	AUX
ajlm-609	29	4	then	then	ADV
ajlm-609	29	5	tested	test	VERB
ajlm-609	29	6	on	on	ADP
ajlm-609	29	7	diarrhoeic	diarrhoeic	VERB
ajlm-609	29	8	stool	stool	NOUN
ajlm-609	29	9	samples	sample	NOUN
ajlm-609	29	10	at	at	ADP
ajlm-609	29	11	a	a	DET
ajlm-609	29	12	tertiary	tertiary	ADJ
ajlm-609	29	13	referral	referral	NOUN
ajlm-609	29	14	hospital	hospital	NOUN
ajlm-609	29	15	and	and	CCONJ
ajlm-609	29	16	was	be	AUX
ajlm-609	29	17	used	use	VERB
ajlm-609	29	18	as	as	ADP
ajlm-609	29	19	a	a	DET
ajlm-609	29	20	reference	reference	NOUN
ajlm-609	29	21	standard	standard	NOUN
ajlm-609	29	22	to	to	PART
ajlm-609	29	23	assess	assess	VERB
ajlm-609	29	24	the	the	DET
ajlm-609	29	25	performance	performance	NOUN
ajlm-609	29	26	of	of	ADP
ajlm-609	29	27	a	a	DET
ajlm-609	29	28	commercial	commercial	ADJ
ajlm-609	29	29	chromogenic	chromogenic	ADJ
ajlm-609	29	30	medium	medium	ADJ
ajlm-609	29	31	(	(	PUNCT
ajlm-609	29	32	chromagartmstec	chromagartmstec	ADJ
ajlm-609	29	33	)	)	PUNCT
ajlm-609	29	34	for	for	ADP
ajlm-609	29	35	stec	stec	ADJ
ajlm-609	29	36	screening	screening	NOUN
ajlm-609	29	37	(	(	PUNCT
ajlm-609	29	38	figure	figure	NOUN
ajlm-609	29	39	1	1	NUM
ajlm-609	29	40	)	)	PUNCT
ajlm-609	29	41	.	.	PUNCT
ajlm-609	30	1	figure	figure	VERB
ajlm-609	30	2	1	1	NUM
ajlm-609	30	3	:	:	PUNCT
ajlm-609	30	4	summary	summary	NOUN
ajlm-609	30	5	of	of	ADP
ajlm-609	30	6	methods	method	NOUN
ajlm-609	30	7	used	use	VERB
ajlm-609	30	8	in	in	ADP
ajlm-609	30	9	this	this	DET
ajlm-609	30	10	study	study	NOUN
ajlm-609	30	11	.	.	PUNCT
ajlm-609	31	1	target	target	NOUN
ajlm-609	31	2	plasmid	plasmid	ADJ
ajlm-609	31	3	preparation	preparation	NOUN
ajlm-609	31	4	the	the	DET
ajlm-609	31	5	real	real	ADJ
ajlm-609	31	6	-	-	PUNCT
ajlm-609	31	7	time	time	NOUN
ajlm-609	31	8	pcr	pcr	NOUN
ajlm-609	31	9	previously	previously	ADV
ajlm-609	31	10	described	describe	VERB
ajlm-609	31	11	by	by	ADP
ajlm-609	31	12	grys	grys	PROPN
ajlm-609	31	13	et	et	PROPN
ajlm-609	31	14	al.16	al.16	PROPN
ajlm-609	31	15	was	be	AUX
ajlm-609	31	16	used	use	VERB
ajlm-609	31	17	to	to	PART
ajlm-609	31	18	amplify	amplify	VERB
ajlm-609	31	19	stx1	stx1	NOUN
ajlm-609	31	20	and	and	CCONJ
ajlm-609	31	21	stx2	stx2	VERB
ajlm-609	31	22	gene	gene	NOUN
ajlm-609	31	23	targets	target	NOUN
ajlm-609	31	24	from	from	ADP
ajlm-609	31	25	a	a	DET
ajlm-609	31	26	stec	stec	ADJ
ajlm-609	31	27	o157	o157	PROPN
ajlm-609	31	28	:	:	PUNCT
ajlm-609	31	29	h7	h7	PROPN
ajlm-609	31	30	nctc	nctc	PROPN
ajlm-609	31	31	control	control	PROPN
ajlm-609	31	32	strain	strain	NOUN
ajlm-609	31	33	(	(	PUNCT
ajlm-609	31	34	c4193	c4193	NOUN
ajlm-609	31	35	-	-	ADJ
ajlm-609	31	36	1	1	NUM
ajlm-609	31	37	)	)	PUNCT
ajlm-609	31	38	with	with	ADP
ajlm-609	31	39	both	both	DET
ajlm-609	31	40	stx1	stx1	NOUN
ajlm-609	31	41	(	(	PUNCT
ajlm-609	31	42	subtype	subtype	NOUN
ajlm-609	31	43	1a	1a	NOUN
ajlm-609	31	44	)	)	PUNCT
ajlm-609	31	45	and	and	CCONJ
ajlm-609	31	46	stx2	stx2	NOUN
ajlm-609	31	47	(	(	PUNCT
ajlm-609	31	48	subtype	subtype	NOUN
ajlm-609	31	49	2a	2a	NUM
ajlm-609	31	50	)	)	PUNCT
ajlm-609	31	51	.	.	PUNCT
ajlm-609	32	1	pcr	pcr	ADJ
ajlm-609	32	2	amplicon	amplicon	NOUN
ajlm-609	32	3	size	size	NOUN
ajlm-609	32	4	was	be	AUX
ajlm-609	32	5	confirmed	confirm	VERB
ajlm-609	32	6	visually	visually	ADV
ajlm-609	32	7	by	by	ADP
ajlm-609	32	8	agarose	agarose	NOUN
ajlm-609	32	9	gel	gel	NOUN
ajlm-609	32	10	detection	detection	NOUN
ajlm-609	32	11	(	(	PUNCT
ajlm-609	32	12	~208	~208	NOUN
ajlm-609	32	13	bp	bp	NOUN
ajlm-609	32	14	for	for	ADP
ajlm-609	32	15	stx1	stx1	NOUN
ajlm-609	32	16	and	and	CCONJ
ajlm-609	32	17	~204	~204	NUM
ajlm-609	32	18	bp	bp	NOUN
ajlm-609	32	19	for	for	ADP
ajlm-609	32	20	stx2	stx2	PROPN
ajlm-609	32	21	)	)	PUNCT
ajlm-609	32	22	before	before	ADP
ajlm-609	32	23	confirmation	confirmation	NOUN
ajlm-609	32	24	by	by	ADP
ajlm-609	32	25	sequencing	sequence	VERB
ajlm-609	32	26	using	use	VERB
ajlm-609	32	27	the	the	DET
ajlm-609	32	28	big	big	ADJ
ajlm-609	32	29	dye	dye	NOUN
ajlm-609	32	30	®	®	NOUN
ajlm-609	32	31	terminator	terminator	NOUN
ajlm-609	32	32	v3.1	v3.1	ADP
ajlm-609	32	33	cycle	cycle	NOUN
ajlm-609	32	34	sequencing	sequence	VERB
ajlm-609	32	35	kit	kit	NOUN
ajlm-609	32	36	(	(	PUNCT
ajlm-609	32	37	life	life	NOUN
ajlm-609	32	38	technologies	technology	NOUN
ajlm-609	32	39	corporation	corporation	PROPN
ajlm-609	32	40	,	,	PUNCT
ajlm-609	32	41	carlsbad	carlsbad	PROPN
ajlm-609	32	42	,	,	PUNCT
ajlm-609	32	43	california	california	PROPN
ajlm-609	32	44	,	,	PUNCT
ajlm-609	32	45	united	united	PROPN
ajlm-609	32	46	states	states	PROPN
ajlm-609	32	47	)	)	PUNCT
ajlm-609	32	48	.	.	PUNCT
ajlm-609	33	1	we	we	PRON
ajlm-609	33	2	used	use	VERB
ajlm-609	33	3	primers	primer	NOUN
ajlm-609	33	4	1a	1a	NOUN
ajlm-609	33	5	and	and	CCONJ
ajlm-609	33	6	2a	2a	NUM
ajlm-609	33	7	(	(	PUNCT
ajlm-609	33	8	table	table	NOUN
ajlm-609	33	9	1	1	NUM
ajlm-609	33	10	)	)	PUNCT
ajlm-609	33	11	for	for	ADP
ajlm-609	33	12	unidirectional	unidirectional	ADJ
ajlm-609	33	13	sanger	sanger	PROPN
ajlm-609	33	14	sequencing	sequencing	NOUN
ajlm-609	33	15	of	of	ADP
ajlm-609	33	16	the	the	DET
ajlm-609	33	17	amplicons	amplicon	NOUN
ajlm-609	33	18	.	.	PUNCT
ajlm-609	34	1	resultant	resultant	VERB
ajlm-609	34	2	sequences	sequence	NOUN
ajlm-609	34	3	were	be	AUX
ajlm-609	34	4	then	then	ADV
ajlm-609	34	5	trimmed	trim	VERB
ajlm-609	34	6	and	and	CCONJ
ajlm-609	34	7	submitted	submit	VERB
ajlm-609	34	8	for	for	ADP
ajlm-609	34	9	blast	blast	NOUN
ajlm-609	34	10	analysis	analysis	NOUN
ajlm-609	34	11	against	against	ADP
ajlm-609	34	12	the	the	DET
ajlm-609	34	13	ncbi	ncbi	PROPN
ajlm-609	34	14	database	database	NOUN
ajlm-609	34	15	and	and	CCONJ
ajlm-609	34	16	confirming	confirm	VERB
ajlm-609	34	17	stx1	stx1	NOUN
ajlm-609	34	18	or	or	CCONJ
ajlm-609	34	19	stx2	stx2	PROPN
ajlm-609	34	20	target	target	NOUN
ajlm-609	34	21	sequences	sequence	NOUN
ajlm-609	34	22	in	in	ADP
ajlm-609	34	23	comparison	comparison	NOUN
ajlm-609	34	24	to	to	ADP
ajlm-609	34	25	o157	o157	PROPN
ajlm-609	34	26	:	:	PUNCT
ajlm-609	34	27	h7	h7	PROPN
ajlm-609	34	28	edl933	edl933	NOUN
ajlm-609	34	29	(	(	PUNCT
ajlm-609	34	30	ncbi	ncbi	PROPN
ajlm-609	34	31	reference	reference	PROPN
ajlm-609	34	32	:	:	PUNCT
ajlm-609	34	33	nc_002655.2).17	nc_002655.2).17	PROPN
ajlm-609	35	1	purified	purified	ADJ
ajlm-609	35	2	amplicons	amplicon	NOUN
ajlm-609	35	3	(	(	PUNCT
ajlm-609	35	4	mini	mini	NOUN
ajlm-609	35	5	elute	elute	NOUN
ajlm-609	35	6	gel	gel	NOUN
ajlm-609	35	7	extraction	extraction	NOUN
ajlm-609	35	8	kit	kit	PROPN
ajlm-609	35	9	,	,	PUNCT
ajlm-609	35	10	qiagen	qiagen	PROPN
ajlm-609	35	11	,	,	PUNCT
ajlm-609	35	12	madrid	madrid	PROPN
ajlm-609	35	13	,	,	PUNCT
ajlm-609	35	14	spain	spain	PROPN
ajlm-609	35	15	)	)	PUNCT
ajlm-609	35	16	were	be	AUX
ajlm-609	35	17	cloned	clone	VERB
ajlm-609	35	18	using	use	VERB
ajlm-609	35	19	clonejet	clonejet	NOUN
ajlm-609	35	20	pcr	pcr	NOUN
ajlm-609	35	21	cloning	cloning	NOUN
ajlm-609	35	22	kit	kit	NOUN
ajlm-609	35	23	(	(	PUNCT
ajlm-609	35	24	thermofisher	thermofisher	PROPN
ajlm-609	35	25	scientific	scientific	PROPN
ajlm-609	35	26	,	,	PUNCT
ajlm-609	35	27	austin	austin	PROPN
ajlm-609	35	28	,	,	PUNCT
ajlm-609	35	29	texas	texas	PROPN
ajlm-609	35	30	,	,	PUNCT
ajlm-609	35	31	united	united	PROPN
ajlm-609	35	32	states	states	PROPN
ajlm-609	35	33	)	)	PUNCT
ajlm-609	35	34	into	into	ADP
ajlm-609	35	35	a	a	DET
ajlm-609	35	36	pjet	pjet	NOUN
ajlm-609	35	37	1.2	1.2	NUM
ajlm-609	35	38	/	/	SYM
ajlm-609	35	39	blunt	blunt	ADJ
ajlm-609	35	40	vector	vector	NOUN
ajlm-609	35	41	using	use	VERB
ajlm-609	35	42	the	the	DET
ajlm-609	35	43	sticky	sticky	ADJ
ajlm-609	35	44	end	end	NOUN
ajlm-609	35	45	cloning	cloning	NOUN
ajlm-609	35	46	protocol	protocol	NOUN
ajlm-609	35	47	and	and	CCONJ
ajlm-609	35	48	transfected	transfecte	VERB
ajlm-609	35	49	into	into	ADP
ajlm-609	35	50	the	the	DET
ajlm-609	35	51	jm109	jm109	PROPN
ajlm-609	35	52	competent	competent	ADJ
ajlm-609	35	53	cells	cell	NOUN
ajlm-609	35	54	by	by	ADP
ajlm-609	35	55	calcium	calcium	NOUN
ajlm-609	35	56	chloride	chloride	NOUN
ajlm-609	35	57	transformation	transformation	NOUN
ajlm-609	35	58	.	.	PUNCT
ajlm-609	36	1	plasmids	plasmid	NOUN
ajlm-609	36	2	containing	contain	VERB
ajlm-609	36	3	stx1	stx1	NOUN
ajlm-609	36	4	and	and	CCONJ
ajlm-609	36	5	stx2	stx2	PROPN
ajlm-609	36	6	were	be	AUX
ajlm-609	36	7	separately	separately	ADV
ajlm-609	36	8	extracted	extract	VERB
ajlm-609	36	9	using	use	VERB
ajlm-609	36	10	a	a	DET
ajlm-609	36	11	genopure	genopure	NOUN
ajlm-609	36	12	plasmid	plasmid	ADJ
ajlm-609	36	13	maxi	maxi	ADJ
ajlm-609	36	14	kit	kit	NOUN
ajlm-609	36	15	(	(	PUNCT
ajlm-609	36	16	roche	roche	NOUN
ajlm-609	36	17	life	life	NOUN
ajlm-609	36	18	sciences	sciences	PROPN
ajlm-609	36	19	,	,	PUNCT
ajlm-609	36	20	rotkreuz	rotkreuz	PROPN
ajlm-609	36	21	,	,	PUNCT
ajlm-609	36	22	switzerland	switzerland	PROPN
ajlm-609	36	23	)	)	PUNCT
ajlm-609	36	24	and	and	CCONJ
ajlm-609	36	25	quantified	quantify	VERB
ajlm-609	36	26	by	by	ADP
ajlm-609	36	27	spectrophotometry	spectrophotometry	NOUN
ajlm-609	36	28	.	.	PUNCT
ajlm-609	37	1	to	to	PART
ajlm-609	37	2	verify	verify	VERB
ajlm-609	37	3	successful	successful	ADJ
ajlm-609	37	4	preparation	preparation	NOUN
ajlm-609	37	5	purified	purify	VERB
ajlm-609	37	6	plasmids	plasmid	NOUN
ajlm-609	37	7	were	be	AUX
ajlm-609	37	8	subjected	subject	VERB
ajlm-609	37	9	to	to	ADP
ajlm-609	37	10	pcr	pcr	ADJ
ajlm-609	37	11	amplification	amplification	NOUN
ajlm-609	37	12	using	use	VERB
ajlm-609	37	13	primers	primer	NOUN
ajlm-609	37	14	1a	1a	NOUN
ajlm-609	37	15	and	and	CCONJ
ajlm-609	37	16	1b	1b	NUM
ajlm-609	37	17	for	for	ADP
ajlm-609	37	18	stx1	stx1	NOUN
ajlm-609	37	19	and	and	CCONJ
ajlm-609	37	20	2a	2a	NUM
ajlm-609	37	21	and	and	CCONJ
ajlm-609	37	22	2b	2b	NOUN
ajlm-609	37	23	for	for	ADP
ajlm-609	37	24	stx2	stx2	NOUN
ajlm-609	37	25	with	with	ADP
ajlm-609	37	26	amplicon	amplicon	NOUN
ajlm-609	37	27	size	size	NOUN
ajlm-609	37	28	visually	visually	ADV
ajlm-609	37	29	confirmed	confirm	VERB
ajlm-609	37	30	by	by	ADP
ajlm-609	37	31	agarose	agarose	NOUN
ajlm-609	37	32	gel	gel	NOUN
ajlm-609	37	33	detection	detection	NOUN
ajlm-609	37	34	and	and	CCONJ
ajlm-609	37	35	subsequent	subsequent	ADJ
ajlm-609	37	36	sequence	sequence	NOUN
ajlm-609	37	37	analysis	analysis	NOUN
ajlm-609	37	38	.	.	PUNCT
ajlm-609	38	1	plasmid	plasmid	ADJ
ajlm-609	38	2	quantification	quantification	NOUN
ajlm-609	38	3	was	be	AUX
ajlm-609	38	4	determined	determine	VERB
ajlm-609	38	5	spectrophotometrically	spectrophotometrically	ADV
ajlm-609	38	6	employing	employ	VERB
ajlm-609	38	7	the	the	DET
ajlm-609	38	8	biodrop	biodrop	NOUN
ajlm-609	38	9	-	-	PUNCT
ajlm-609	38	10	μlite	μlite	NOUN
ajlm-609	38	11	(	(	PUNCT
ajlm-609	38	12	isogen	isogen	NOUN
ajlm-609	38	13	life	life	NOUN
ajlm-609	38	14	science	science	NOUN
ajlm-609	38	15	,	,	PUNCT
ajlm-609	38	16	b.v	b.v	PROPN
ajlm-609	38	17	,	,	PUNCT
ajlm-609	38	18	veldzigt	veldzigt	ADJ
ajlm-609	38	19	,	,	PUNCT
ajlm-609	38	20	netherlands	netherlands	PROPN
ajlm-609	38	21	)	)	PUNCT
ajlm-609	38	22	.	.	PUNCT
ajlm-609	39	1	the	the	DET
ajlm-609	39	2	a260	a260	PROPN
ajlm-609	39	3	was	be	AUX
ajlm-609	39	4	used	use	VERB
ajlm-609	39	5	to	to	PART
ajlm-609	39	6	calculate	calculate	VERB
ajlm-609	39	7	the	the	DET
ajlm-609	39	8	plasmid	plasmid	ADJ
ajlm-609	39	9	concentration	concentration	NOUN
ajlm-609	39	10	expressed	express	VERB
ajlm-609	39	11	as	as	ADP
ajlm-609	39	12	the	the	DET
ajlm-609	39	13	number	number	NOUN
ajlm-609	39	14	of	of	ADP
ajlm-609	39	15	molecules	molecule	NOUN
ajlm-609	39	16	/	/	SYM
ajlm-609	39	17	μl	μl	NOUN
ajlm-609	39	18	.	.	PUNCT
ajlm-609	39	19	table	table	NOUN
ajlm-609	39	20	1	1	NUM
ajlm-609	39	21	:	:	PUNCT
ajlm-609	39	22	 	 	SPACE
ajlm-609	39	23	primers	primer	NOUN
ajlm-609	39	24	and	and	CCONJ
ajlm-609	39	25	probes	probe	NOUN
ajlm-609	39	26	used	use	VERB
ajlm-609	39	27	for	for	ADP
ajlm-609	39	28	real	real	ADJ
ajlm-609	39	29	-	-	PUNCT
ajlm-609	39	30	time	time	NOUN
ajlm-609	39	31	polymerase	polymerase	NOUN
ajlm-609	39	32	chain	chain	NOUN
ajlm-609	39	33	reaction	reaction	NOUN
ajlm-609	39	34	.	.	PUNCT
ajlm-609	40	1	polymerase	polymerase	NOUN
ajlm-609	40	2	chain	chain	NOUN
ajlm-609	40	3	reaction	reaction	NOUN
ajlm-609	40	4	assay	assay	VERB
ajlm-609	40	5	validation	validation	NOUN
ajlm-609	40	6	to	to	PART
ajlm-609	40	7	assess	assess	VERB
ajlm-609	40	8	the	the	DET
ajlm-609	40	9	potential	potential	NOUN
ajlm-609	40	10	for	for	ADP
ajlm-609	40	11	pcr	pcr	ADJ
ajlm-609	40	12	cross	cross	ADJ
ajlm-609	40	13	-	-	NOUN
ajlm-609	40	14	reactivity	reactivity	NOUN
ajlm-609	40	15	and	and	CCONJ
ajlm-609	40	16	assess	assess	VERB
ajlm-609	40	17	the	the	DET
ajlm-609	40	18	analytical	analytical	ADJ
ajlm-609	40	19	specificity	specificity	NOUN
ajlm-609	40	20	of	of	ADP
ajlm-609	40	21	the	the	DET
ajlm-609	40	22	hybridisation	hybridisation	NOUN
ajlm-609	40	23	probe	probe	NOUN
ajlm-609	40	24	-	-	PUNCT
ajlm-609	40	25	based	base	VERB
ajlm-609	40	26	real	real	ADJ
ajlm-609	40	27	-	-	PUNCT
ajlm-609	40	28	time	time	NOUN
ajlm-609	40	29	pcr	pcr	NOUN
ajlm-609	40	30	described	describe	VERB
ajlm-609	40	31	by	by	ADP
ajlm-609	40	32	grys	grys	PROPN
ajlm-609	40	33	et	et	PROPN
ajlm-609	40	34	al	al	PROPN
ajlm-609	40	35	.	.	PROPN
ajlm-609	40	36	,16	,16	PUNCT
ajlm-609	40	37	the	the	DET
ajlm-609	40	38	primer	primer	NOUN
ajlm-609	40	39	and	and	CCONJ
ajlm-609	40	40	probe	probe	NOUN
ajlm-609	40	41	sequences	sequence	NOUN
ajlm-609	40	42	were	be	AUX
ajlm-609	40	43	subjected	subject	VERB
ajlm-609	40	44	to	to	PART
ajlm-609	40	45	blast	blast	VERB
ajlm-609	40	46	analysis	analysis	NOUN
ajlm-609	40	47	on	on	ADP
ajlm-609	40	48	the	the	DET
ajlm-609	40	49	ncbi	ncbi	PROPN
ajlm-609	40	50	database	database	NOUN
ajlm-609	40	51	.	.	PUNCT
ajlm-609	41	1	the	the	DET
ajlm-609	41	2	pcr	pcr	PROPN
ajlm-609	41	3	reaction	reaction	NOUN
ajlm-609	41	4	was	be	AUX
ajlm-609	41	5	optimised	optimise	VERB
ajlm-609	41	6	for	for	ADP
ajlm-609	41	7	use	use	NOUN
ajlm-609	41	8	on	on	ADP
ajlm-609	41	9	the	the	DET
ajlm-609	41	10	lightcycler	lightcycler	NOUN
ajlm-609	41	11	®	®	PROPN
ajlm-609	41	12	480	480	NUM
ajlm-609	41	13	instrument	instrument	PROPN
ajlm-609	41	14	ii	ii	PROPN
ajlm-609	41	15	(	(	PUNCT
ajlm-609	41	16	roche	roche	PROPN
ajlm-609	41	17	life	life	NOUN
ajlm-609	41	18	sciences	sciences	PROPN
ajlm-609	41	19	,	,	PUNCT
ajlm-609	41	20	rotkreuz	rotkreuz	PROPN
ajlm-609	41	21	,	,	PUNCT
ajlm-609	41	22	switzerland	switzerland	PROPN
ajlm-609	41	23	)	)	PUNCT
ajlm-609	41	24	employing	employ	VERB
ajlm-609	41	25	the	the	DET
ajlm-609	41	26	lightcycler	lightcycler	ADJ
ajlm-609	41	27	®	®	NOUN
ajlm-609	41	28	480	480	NUM
ajlm-609	41	29	probes	probe	NOUN
ajlm-609	41	30	master	master	NOUN
ajlm-609	41	31	mastermix	mastermix	NOUN
ajlm-609	41	32	(	(	PUNCT
ajlm-609	41	33	roche	roche	X
ajlm-609	41	34	life	life	NOUN
ajlm-609	41	35	sciences	sciences	PROPN
ajlm-609	41	36	,	,	PUNCT
ajlm-609	41	37	rotkreuz	rotkreuz	PROPN
ajlm-609	41	38	,	,	PUNCT
ajlm-609	41	39	switzerland	switzerland	PROPN
ajlm-609	41	40	)	)	PUNCT
ajlm-609	41	41	with	with	ADP
ajlm-609	41	42	modification	modification	NOUN
ajlm-609	41	43	to	to	ADP
ajlm-609	41	44	the	the	DET
ajlm-609	41	45	thermal	thermal	ADJ
ajlm-609	41	46	cycling	cycling	NOUN
ajlm-609	41	47	conditions	condition	NOUN
ajlm-609	41	48	for	for	ADP
ajlm-609	41	49	amplification	amplification	NOUN
ajlm-609	41	50	consisting	consist	VERB
ajlm-609	41	51	of	of	ADP
ajlm-609	41	52	denaturation	denaturation	NOUN
ajlm-609	41	53	at	at	ADP
ajlm-609	41	54	95	95	NUM
ajlm-609	41	55	°	°	ADP
ajlm-609	41	56	c	c	NOUN
ajlm-609	41	57	for	for	ADP
ajlm-609	41	58	10	10	NUM
ajlm-609	41	59	min	min	NOUN
ajlm-609	41	60	followed	follow	VERB
ajlm-609	41	61	by	by	ADP
ajlm-609	41	62	45	45	NUM
ajlm-609	41	63	cycles	cycle	NOUN
ajlm-609	41	64	of	of	ADP
ajlm-609	41	65	95	95	NUM
ajlm-609	41	66	°	°	ADP
ajlm-609	41	67	c	c	NOUN
ajlm-609	41	68	for	for	ADP
ajlm-609	41	69	5	5	NUM
ajlm-609	41	70	s	s	NOUN
ajlm-609	41	71	,	,	PUNCT
ajlm-609	41	72	56	56	NUM
ajlm-609	41	73	°	°	NUM
ajlm-609	41	74	c	c	NOUN
ajlm-609	41	75	for	for	ADP
ajlm-609	41	76	5	5	NUM
ajlm-609	41	77	s	s	NOUN
ajlm-609	41	78	and	and	CCONJ
ajlm-609	41	79	72	72	NUM
ajlm-609	41	80	°	°	NUM
ajlm-609	41	81	c	c	NOUN
ajlm-609	41	82	for	for	ADP
ajlm-609	41	83	15	15	NUM
ajlm-609	41	84	s.	s.	PROPN
ajlm-609	41	85	a	a	DET
ajlm-609	41	86	positive	positive	ADJ
ajlm-609	41	87	amplification	amplification	NOUN
ajlm-609	41	88	signal	signal	NOUN
ajlm-609	41	89	was	be	AUX
ajlm-609	41	90	defined	define	VERB
ajlm-609	41	91	as	as	ADP
ajlm-609	41	92	an	an	DET
ajlm-609	41	93	increase	increase	NOUN
ajlm-609	41	94	in	in	ADP
ajlm-609	41	95	fluorescence	fluorescence	NOUN
ajlm-609	41	96	signal	signal	NOUN
ajlm-609	41	97	that	that	PRON
ajlm-609	41	98	crossed	cross	VERB
ajlm-609	41	99	the	the	DET
ajlm-609	41	100	threshold	threshold	NOUN
ajlm-609	41	101	before	before	ADP
ajlm-609	41	102	30	30	NUM
ajlm-609	41	103	cycles	cycle	NOUN
ajlm-609	41	104	.	.	PUNCT
ajlm-609	42	1	amplicon	amplicon	NOUN
ajlm-609	42	2	identity	identity	NOUN
ajlm-609	42	3	was	be	AUX
ajlm-609	42	4	determined	determine	VERB
ajlm-609	42	5	using	use	VERB
ajlm-609	42	6	the	the	DET
ajlm-609	42	7	melt	melt	NOUN
ajlm-609	42	8	-	-	PUNCT
ajlm-609	42	9	curve	curve	NOUN
ajlm-609	42	10	analysis	analysis	NOUN
ajlm-609	42	11	program	program	NOUN
ajlm-609	42	12	of	of	ADP
ajlm-609	42	13	95	95	NUM
ajlm-609	42	14	°	°	ADP
ajlm-609	42	15	c	c	NOUN
ajlm-609	42	16	for	for	ADP
ajlm-609	42	17	30	30	NUM
ajlm-609	42	18	s	s	NOUN
ajlm-609	42	19	,	,	PUNCT
ajlm-609	42	20	40	40	NUM
ajlm-609	42	21	°	°	NOUN
ajlm-609	42	22	c	c	NOUN
ajlm-609	42	23	for	for	ADP
ajlm-609	42	24	60	60	NUM
ajlm-609	42	25	s	s	NOUN
ajlm-609	42	26	and	and	CCONJ
ajlm-609	43	1	85	85	NUM
ajlm-609	43	2	°	°	NOUN
ajlm-609	43	3	c	c	NOUN
ajlm-609	43	4	for	for	ADP
ajlm-609	43	5	5	5	NUM
ajlm-609	43	6	s	s	NOUN
ajlm-609	43	7	with	with	ADP
ajlm-609	43	8	continuous	continuous	ADJ
ajlm-609	43	9	fluorescence	fluorescence	NOUN
ajlm-609	43	10	acquisition	acquisition	NOUN
ajlm-609	43	11	.	.	PUNCT
ajlm-609	44	1	the	the	DET
ajlm-609	44	2	multi	multi	ADJ
ajlm-609	44	3	-	-	ADJ
ajlm-609	44	4	color	color	ADJ
ajlm-609	44	5	hybprobe	hybprobe	NOUN
ajlm-609	44	6	detection	detection	NOUN
ajlm-609	44	7	format	format	NOUN
ajlm-609	44	8	was	be	AUX
ajlm-609	44	9	used	use	VERB
ajlm-609	44	10	for	for	ADP
ajlm-609	44	11	analysis	analysis	NOUN
ajlm-609	44	12	,	,	PUNCT
ajlm-609	44	13	combining	combine	VERB
ajlm-609	44	14	the	the	DET
ajlm-609	44	15	red	red	ADJ
ajlm-609	44	16	610	610	NUM
ajlm-609	44	17	,	,	PUNCT
ajlm-609	44	18	red	red	NOUN
ajlm-609	44	19	640	640	NUM
ajlm-609	44	20	and	and	CCONJ
ajlm-609	44	21	fam	fam	NOUN
ajlm-609	44	22	filter	filter	NOUN
ajlm-609	44	23	pairs	pair	NOUN
ajlm-609	44	24	(	(	PUNCT
ajlm-609	44	25	lightcycler	lightcycler	NOUN
ajlm-609	44	26	®	®	PROPN
ajlm-609	44	27	480	480	NUM
ajlm-609	44	28	instrument	instrument	PROPN
ajlm-609	44	29	ii	ii	PROPN
ajlm-609	44	30	manual	manual	NOUN
ajlm-609	44	31	,	,	PUNCT
ajlm-609	44	32	roche	roche	PROPN
ajlm-609	44	33	life	life	NOUN
ajlm-609	44	34	sciences	sciences	PROPN
ajlm-609	44	35	,	,	PUNCT
ajlm-609	44	36	rotkreuz	rotkreuz	PROPN
ajlm-609	44	37	,	,	PUNCT
ajlm-609	44	38	switzerland	switzerland	PROPN
ajlm-609	44	39	)	)	PUNCT
ajlm-609	44	40	.	.	PUNCT
ajlm-609	45	1	the	the	DET
ajlm-609	45	2	resulting	result	VERB
ajlm-609	45	3	amplicon	amplicon	NOUN
ajlm-609	45	4	size	size	NOUN
ajlm-609	45	5	was	be	AUX
ajlm-609	45	6	visualised	visualise	VERB
ajlm-609	45	7	using	use	VERB
ajlm-609	45	8	agarose	agarose	NOUN
ajlm-609	45	9	gel	gel	NOUN
ajlm-609	45	10	electrophoresis	electrophoresis	NOUN
ajlm-609	45	11	and	and	CCONJ
ajlm-609	45	12	subjected	subject	VERB
ajlm-609	45	13	to	to	ADP
ajlm-609	45	14	dna	dna	NOUN
ajlm-609	45	15	sequencing	sequencing	NOUN
ajlm-609	45	16	and	and	CCONJ
ajlm-609	45	17	blast	blast	NOUN
ajlm-609	45	18	alignment	alignment	NOUN
ajlm-609	45	19	to	to	PART
ajlm-609	45	20	reference	reference	NOUN
ajlm-609	45	21	stx1a	stx1a	ADV
ajlm-609	45	22	and	and	CCONJ
ajlm-609	45	23	stx2a	stx2a	PROPN
ajlm-609	45	24	sequences	sequence	NOUN
ajlm-609	45	25	(	(	PUNCT
ajlm-609	45	26	nc_002655.2	nc_002655.2	NUM
ajlm-609	45	27	)	)	PUNCT
ajlm-609	45	28	.	.	PUNCT
ajlm-609	46	1	to	to	PART
ajlm-609	46	2	mimic	mimic	VERB
ajlm-609	46	3	the	the	DET
ajlm-609	46	4	sample	sample	NOUN
ajlm-609	46	5	matrix	matrix	NOUN
ajlm-609	46	6	for	for	ADP
ajlm-609	46	7	sensitivity	sensitivity	NOUN
ajlm-609	46	8	determination	determination	NOUN
ajlm-609	46	9	,	,	PUNCT
ajlm-609	46	10	tsb	tsb	PROPN
ajlm-609	46	11	was	be	AUX
ajlm-609	46	12	inoculated	inoculate	VERB
ajlm-609	46	13	with	with	ADP
ajlm-609	46	14	a	a	DET
ajlm-609	46	15	pea	pea	NOUN
ajlm-609	46	16	-	-	PUNCT
ajlm-609	46	17	size	size	NOUN
ajlm-609	46	18	amount	amount	NOUN
ajlm-609	46	19	of	of	ADP
ajlm-609	46	20	stool	stool	NOUN
ajlm-609	46	21	(	(	PUNCT
ajlm-609	46	22	from	from	ADP
ajlm-609	46	23	a	a	DET
ajlm-609	46	24	single	single	ADJ
ajlm-609	46	25	donor	donor	NOUN
ajlm-609	46	26	)	)	PUNCT
ajlm-609	46	27	shown	show	VERB
ajlm-609	46	28	to	to	PART
ajlm-609	46	29	be	be	AUX
ajlm-609	46	30	stx	stx	NOUN
ajlm-609	46	31	-	-	PUNCT
ajlm-609	46	32	negative	negative	ADJ
ajlm-609	46	33	by	by	ADP
ajlm-609	46	34	pcr	pcr	PROPN
ajlm-609	46	35	.	.	PUNCT
ajlm-609	47	1	to	to	ADP
ajlm-609	47	2	this	this	DET
ajlm-609	47	3	inoculated	inoculate	VERB
ajlm-609	47	4	broth	broth	NOUN
ajlm-609	47	5	1	1	NUM
ajlm-609	47	6	ml	ml	NOUN
ajlm-609	47	7	of	of	ADP
ajlm-609	47	8	plasmid	plasmid	NOUN
ajlm-609	47	9	stock	stock	NOUN
ajlm-609	47	10	(	(	PUNCT
ajlm-609	47	11	5.3	5.3	NUM
ajlm-609	47	12	*	*	SYM
ajlm-609	47	13	106	106	NUM
ajlm-609	47	14	copies	copy	NOUN
ajlm-609	47	15	/	/	SYM
ajlm-609	47	16	μl	μl	CCONJ
ajlm-609	47	17	)	)	PUNCT
ajlm-609	47	18	containing	contain	VERB
ajlm-609	47	19	both	both	CCONJ
ajlm-609	47	20	stx1	stx1	NOUN
ajlm-609	47	21	and	and	CCONJ
ajlm-609	47	22	stx2	stx2	PROPN
ajlm-609	47	23	was	be	AUX
ajlm-609	47	24	added	add	VERB
ajlm-609	47	25	and	and	CCONJ
ajlm-609	47	26	serially	serially	ADV
ajlm-609	47	27	diluted	dilute	VERB
ajlm-609	47	28	eight	eight	NUM
ajlm-609	47	29	times	time	NOUN
ajlm-609	47	30	in	in	ADP
ajlm-609	47	31	9	9	NUM
ajlm-609	47	32	ml	ml	NOUN
ajlm-609	47	33	of	of	ADP
ajlm-609	47	34	tsb	tsb	NOUN
ajlm-609	47	35	,	,	PUNCT
ajlm-609	47	36	to	to	ADP
ajlm-609	47	37	a	a	DET
ajlm-609	47	38	lowest	low	ADJ
ajlm-609	47	39	dilution	dilution	NOUN
ajlm-609	47	40	of	of	ADP
ajlm-609	47	41	1:108	1:108	NUM
ajlm-609	47	42	(	(	PUNCT
ajlm-609	47	43	53	53	NUM
ajlm-609	47	44	plasmid	plasmid	ADJ
ajlm-609	47	45	copies	copy	NOUN
ajlm-609	47	46	/	/	SYM
ajlm-609	47	47	ml	ml	NOUN
ajlm-609	47	48	)	)	PUNCT
ajlm-609	47	49	.	.	PUNCT
ajlm-609	48	1	nucleic	nucleic	ADJ
ajlm-609	48	2	acid	acid	NOUN
ajlm-609	48	3	extraction	extraction	NOUN
ajlm-609	48	4	was	be	AUX
ajlm-609	48	5	performed	perform	VERB
ajlm-609	48	6	on	on	ADP
ajlm-609	48	7	200	200	NUM
ajlm-609	48	8	μl	μl	DET
ajlm-609	48	9	broth	broth	NOUN
ajlm-609	48	10	employing	employ	VERB
ajlm-609	48	11	the	the	DET
ajlm-609	48	12	magnapure	magnapure	ADJ
ajlm-609	48	13	lc	lc	PROPN
ajlm-609	48	14	instrument	instrument	PROPN
ajlm-609	48	15	(	(	PUNCT
ajlm-609	48	16	roche	roche	NOUN
ajlm-609	48	17	diagnostics	diagnostic	NOUN
ajlm-609	48	18	,	,	PUNCT
ajlm-609	48	19	rotkreuz	rotkreuz	PROPN
ajlm-609	48	20	,	,	PUNCT
ajlm-609	48	21	switzerland	switzerland	PROPN
ajlm-609	48	22	)	)	PUNCT
ajlm-609	48	23	to	to	PART
ajlm-609	48	24	yield	yield	VERB
ajlm-609	48	25	100	100	NUM
ajlm-609	48	26	μl	μl	ADP
ajlm-609	48	27	of	of	ADP
ajlm-609	48	28	extract	extract	NOUN
ajlm-609	48	29	.	.	PUNCT
ajlm-609	49	1	initially	initially	ADV
ajlm-609	49	2	,	,	PUNCT
ajlm-609	49	3	real	real	ADJ
ajlm-609	49	4	-	-	PUNCT
ajlm-609	49	5	time	time	NOUN
ajlm-609	49	6	pcr	pcr	NOUN
ajlm-609	49	7	was	be	AUX
ajlm-609	49	8	performed	perform	VERB
ajlm-609	49	9	in	in	ADP
ajlm-609	49	10	triplicate	triplicate	NOUN
ajlm-609	49	11	using	use	VERB
ajlm-609	49	12	a	a	DET
ajlm-609	49	13	template	template	NOUN
ajlm-609	49	14	from	from	ADP
ajlm-609	49	15	each	each	PRON
ajlm-609	49	16	of	of	ADP
ajlm-609	49	17	the	the	DET
ajlm-609	49	18	eight	eight	NUM
ajlm-609	49	19	dilutions	dilution	NOUN
ajlm-609	49	20	to	to	PART
ajlm-609	49	21	estimate	estimate	VERB
ajlm-609	49	22	a	a	DET
ajlm-609	49	23	limit	limit	NOUN
ajlm-609	49	24	of	of	ADP
ajlm-609	49	25	detection	detection	NOUN
ajlm-609	49	26	(	(	PUNCT
ajlm-609	49	27	lod	lod	PROPN
ajlm-609	49	28	)	)	PUNCT
ajlm-609	49	29	.	.	PUNCT
ajlm-609	50	1	subsequently	subsequently	ADV
ajlm-609	50	2	,	,	PUNCT
ajlm-609	50	3	real	real	ADJ
ajlm-609	50	4	-	-	PUNCT
ajlm-609	50	5	time	time	NOUN
ajlm-609	50	6	pcr	pcr	NOUN
ajlm-609	50	7	was	be	AUX
ajlm-609	50	8	performed	perform	VERB
ajlm-609	50	9	in	in	ADP
ajlm-609	50	10	eight	eight	NUM
ajlm-609	50	11	replicates	replicate	NOUN
ajlm-609	50	12	on	on	ADP
ajlm-609	50	13	the	the	DET
ajlm-609	50	14	dilution	dilution	NOUN
ajlm-609	50	15	with	with	ADP
ajlm-609	50	16	the	the	DET
ajlm-609	50	17	estimated	estimate	VERB
ajlm-609	50	18	lod	lod	PROPN
ajlm-609	50	19	,	,	PUNCT
ajlm-609	50	20	as	as	ADV
ajlm-609	50	21	well	well	ADV
ajlm-609	50	22	as	as	ADP
ajlm-609	50	23	one	one	NUM
ajlm-609	50	24	dilution	dilution	NOUN
ajlm-609	50	25	above	above	ADV
ajlm-609	50	26	and	and	CCONJ
ajlm-609	50	27	one	one	NUM
ajlm-609	50	28	dilution	dilution	NOUN
ajlm-609	50	29	below	below	ADP
ajlm-609	50	30	the	the	DET
ajlm-609	50	31	estimate	estimate	NOUN
ajlm-609	50	32	.	.	PUNCT
ajlm-609	51	1	the	the	DET
ajlm-609	51	2	lod	lod	PROPN
ajlm-609	51	3	was	be	AUX
ajlm-609	51	4	defined	define	VERB
ajlm-609	51	5	as	as	ADP
ajlm-609	51	6	the	the	DET
ajlm-609	51	7	lowest	low	ADJ
ajlm-609	51	8	plasmid	plasmid	ADJ
ajlm-609	51	9	concentration	concentration	NOUN
ajlm-609	51	10	spiked	spike	VERB
ajlm-609	51	11	into	into	ADP
ajlm-609	51	12	tsb	tsb	NOUN
ajlm-609	51	13	,	,	PUNCT
ajlm-609	51	14	before	before	ADP
ajlm-609	51	15	nucleic	nucleic	ADJ
ajlm-609	51	16	acid	acid	NOUN
ajlm-609	51	17	extraction	extraction	NOUN
ajlm-609	51	18	,	,	PUNCT
ajlm-609	51	19	yielding	yield	VERB
ajlm-609	51	20	a	a	DET
ajlm-609	51	21	positive	positive	ADJ
ajlm-609	51	22	signal	signal	NOUN
ajlm-609	51	23	,	,	PUNCT
ajlm-609	51	24	as	as	SCONJ
ajlm-609	51	25	described	describe	VERB
ajlm-609	51	26	above	above	ADV
ajlm-609	51	27	in	in	ADP
ajlm-609	51	28	all	all	DET
ajlm-609	51	29	eight	eight	NUM
ajlm-609	51	30	replicates	replicate	NOUN
ajlm-609	51	31	.	.	PUNCT
ajlm-609	52	1	nucleic	nucleic	ADJ
ajlm-609	52	2	acid	acid	NOUN
ajlm-609	52	3	extractions	extraction	NOUN
ajlm-609	52	4	from	from	ADP
ajlm-609	52	5	stec	stec	ADJ
ajlm-609	52	6	subtypes	subtype	NOUN
ajlm-609	52	7	1d	1d	NUM
ajlm-609	52	8	(	(	PUNCT
ajlm-609	52	9	reference	reference	NOUN
ajlm-609	52	10	strain	strain	VERB
ajlm-609	52	11	mh1813	mh1813	ADJ
ajlm-609	52	12	,	,	PUNCT
ajlm-609	52	13	genbank	genbank	NOUN
ajlm-609	52	14	accession	accession	NOUN
ajlm-609	53	1	no	no	PROPN
ajlm-609	53	2	.	.	PUNCT
ajlm-609	54	1	ay170851	ay170851	PROPN
ajlm-609	54	2	)	)	PUNCT
ajlm-609	54	3	,	,	PUNCT
ajlm-609	54	4	2b	2b	NUM
ajlm-609	54	5	(	(	PUNCT
ajlm-609	54	6	reference	reference	NOUN
ajlm-609	54	7	strain	strain	NOUN
ajlm-609	54	8	eh250	eh250	PROPN
ajlm-609	54	9	,	,	PUNCT
ajlm-609	54	10	genbank	genbank	NOUN
ajlm-609	54	11	accession	accession	NOUN
ajlm-609	55	1	no	no	INTJ
ajlm-609	55	2	.	.	PUNCT
ajlm-609	56	1	af043627	af043627	PROPN
ajlm-609	56	2	)	)	PUNCT
ajlm-609	56	3	,	,	PUNCT
ajlm-609	56	4	2c	2c	NOUN
ajlm-609	56	5	(	(	PUNCT
ajlm-609	56	6	reference	reference	NOUN
ajlm-609	56	7	strain	strain	NOUN
ajlm-609	56	8	031	031	NUM
ajlm-609	56	9	,	,	PUNCT
ajlm-609	56	10	genbank	genbank	NOUN
ajlm-609	56	11	accession	accession	NOUN
ajlm-609	56	12	no	no	INTJ
ajlm-609	56	13	.	.	PUNCT
ajlm-609	57	1	l11079	l11079	NOUN
ajlm-609	57	2	)	)	PUNCT
ajlm-609	57	3	,	,	PUNCT
ajlm-609	57	4	2d	2d	NUM
ajlm-609	57	5	(	(	PUNCT
ajlm-609	57	6	reference	reference	NOUN
ajlm-609	57	7	strain	strain	VERB
ajlm-609	57	8	c165	c165	PROPN
ajlm-609	57	9	-	-	PUNCT
ajlm-609	57	10	02	02	NUM
ajlm-609	57	11	,	,	PUNCT
ajlm-609	57	12	genbank	genbank	NOUN
ajlm-609	57	13	accession	accession	NOUN
ajlm-609	57	14	no	no	PROPN
ajlm-609	57	15	.	.	PUNCT
ajlm-609	57	16	dq059012	dq059012	PROPN
ajlm-609	57	17	)	)	PUNCT
ajlm-609	57	18	,	,	PUNCT
ajlm-609	57	19	2e	2e	NUM
ajlm-609	57	20	(	(	PUNCT
ajlm-609	57	21	reference	reference	NOUN
ajlm-609	57	22	strain	strain	NOUN
ajlm-609	57	23	s1191	s1191	PROPN
ajlm-609	57	24	,	,	PUNCT
ajlm-609	57	25	genbank	genbank	NOUN
ajlm-609	57	26	accession	accession	NOUN
ajlm-609	58	1	no	no	INTJ
ajlm-609	58	2	.	.	PUNCT
ajlm-609	59	1	m21534	m21534	NOUN
ajlm-609	59	2	)	)	PUNCT
ajlm-609	59	3	,	,	PUNCT
ajlm-609	59	4	2f	2f	NUM
ajlm-609	59	5	(	(	PUNCT
ajlm-609	59	6	reference	reference	NOUN
ajlm-609	59	7	strain	strain	VERB
ajlm-609	59	8	t4/97	t4/97	NUM
ajlm-609	59	9	,	,	PUNCT
ajlm-609	59	10	genbank	genbank	NOUN
ajlm-609	59	11	accession	accession	NOUN
ajlm-609	60	1	no	no	INTJ
ajlm-609	60	2	.	.	PUNCT
ajlm-609	61	1	aj010730	aj010730	PROPN
ajlm-609	61	2	)	)	PUNCT
ajlm-609	61	3	and	and	CCONJ
ajlm-609	61	4	2	2	NUM
ajlm-609	61	5	g	g	NOUN
ajlm-609	61	6	(	(	PUNCT
ajlm-609	61	7	reference	reference	NOUN
ajlm-609	61	8	strain	strain	VERB
ajlm-609	61	9	7v	7v	NOUN
ajlm-609	61	10	,	,	PUNCT
ajlm-609	61	11	genbank	genbank	NOUN
ajlm-609	61	12	accession	accession	NOUN
ajlm-609	61	13	no	no	INTJ
ajlm-609	61	14	.	.	PUNCT
ajlm-609	62	1	ay286000	ay286000	PROPN
ajlm-609	62	2	)	)	PUNCT
ajlm-609	62	3	were	be	AUX
ajlm-609	62	4	also	also	ADV
ajlm-609	62	5	subjected	subject	VERB
ajlm-609	62	6	to	to	ADP
ajlm-609	62	7	pcr	pcr	ADJ
ajlm-609	62	8	amplification	amplification	NOUN
ajlm-609	62	9	to	to	PART
ajlm-609	62	10	assess	assess	VERB
ajlm-609	62	11	impact	impact	NOUN
ajlm-609	62	12	of	of	ADP
ajlm-609	62	13	strain	strain	ADJ
ajlm-609	62	14	variation	variation	NOUN
ajlm-609	62	15	on	on	ADP
ajlm-609	62	16	detection	detection	NOUN
ajlm-609	62	17	.	.	PUNCT
ajlm-609	63	1	the	the	DET
ajlm-609	63	2	reproducibility	reproducibility	NOUN
ajlm-609	63	3	of	of	ADP
ajlm-609	63	4	melting	melt	VERB
ajlm-609	63	5	temperature	temperature	NOUN
ajlm-609	63	6	assessment	assessment	NOUN
ajlm-609	63	7	for	for	ADP
ajlm-609	63	8	stx1	stx1	NOUN
ajlm-609	63	9	and	and	CCONJ
ajlm-609	63	10	stx2	stx2	PROPN
ajlm-609	63	11	differentiation	differentiation	NOUN
ajlm-609	63	12	was	be	AUX
ajlm-609	63	13	determined	determine	VERB
ajlm-609	63	14	by	by	ADP
ajlm-609	63	15	testing	test	VERB
ajlm-609	63	16	24	24	NUM
ajlm-609	63	17	replicates	replicate	NOUN
ajlm-609	63	18	of	of	ADP
ajlm-609	63	19	tsb	tsb	NOUN
ajlm-609	63	20	spiked	spike	VERB
ajlm-609	63	21	with	with	ADP
ajlm-609	63	22	cloned	cloned	ADJ
ajlm-609	63	23	stx1	stx1	NOUN
ajlm-609	63	24	and	and	CCONJ
ajlm-609	63	25	stx2	stx2	VERB
ajlm-609	63	26	plasmids	plasmid	NOUN
ajlm-609	63	27	.	.	PUNCT
ajlm-609	64	1	to	to	PART
ajlm-609	64	2	further	far	ADV
ajlm-609	64	3	assess	assess	VERB
ajlm-609	64	4	the	the	DET
ajlm-609	64	5	reproducibility	reproducibility	NOUN
ajlm-609	64	6	of	of	ADP
ajlm-609	64	7	melting	melt	VERB
ajlm-609	64	8	temperature	temperature	NOUN
ajlm-609	64	9	across	across	ADP
ajlm-609	64	10	the	the	DET
ajlm-609	64	11	subtypes	subtype	NOUN
ajlm-609	64	12	,	,	PUNCT
ajlm-609	64	13	three	three	NUM
ajlm-609	64	14	stx1	stx1	NOUN
ajlm-609	64	15	subtypes	subtype	NOUN
ajlm-609	64	16	and	and	CCONJ
ajlm-609	64	17	seven	seven	NUM
ajlm-609	64	18	stx2	stx2	PROPN
ajlm-609	64	19	subtypes	subtype	NOUN
ajlm-609	64	20	were	be	AUX
ajlm-609	64	21	tested	test	VERB
ajlm-609	64	22	similarly	similarly	ADV
ajlm-609	64	23	.	.	PUNCT
ajlm-609	65	1	clinical	clinical	ADJ
ajlm-609	65	2	specimen	speciman	NOUN
ajlm-609	65	3	testing	test	VERB
ajlm-609	65	4	between	between	ADP
ajlm-609	65	5	september	september	PROPN
ajlm-609	65	6	2014	2014	NUM
ajlm-609	65	7	and	and	CCONJ
ajlm-609	65	8	may	may	PROPN
ajlm-609	65	9	2015	2015	NUM
ajlm-609	65	10	,	,	PUNCT
ajlm-609	65	11	we	we	PRON
ajlm-609	65	12	collected	collect	VERB
ajlm-609	65	13	same	same	ADJ
ajlm-609	65	14	day	day	NOUN
ajlm-609	65	15	residual	residual	ADJ
ajlm-609	65	16	stool	stool	NOUN
ajlm-609	65	17	after	after	ADP
ajlm-609	65	18	routine	routine	ADJ
ajlm-609	65	19	testing	testing	NOUN
ajlm-609	65	20	from	from	ADP
ajlm-609	65	21	226	226	NUM
ajlm-609	65	22	consecutive	consecutive	ADJ
ajlm-609	65	23	stool	stool	NOUN
ajlm-609	65	24	specimens	specimen	NOUN
ajlm-609	65	25	(	(	PUNCT
ajlm-609	65	26	the	the	DET
ajlm-609	65	27	stool	stool	NOUN
ajlm-609	65	28	samples	sample	NOUN
ajlm-609	65	29	were	be	AUX
ajlm-609	65	30	transported	transport	VERB
ajlm-609	65	31	in	in	ADP
ajlm-609	65	32	a	a	DET
ajlm-609	65	33	temperature	temperature	NOUN
ajlm-609	65	34	regulated	regulate	VERB
ajlm-609	65	35	box	box	NOUN
ajlm-609	65	36	and	and	CCONJ
ajlm-609	65	37	processed	process	VERB
ajlm-609	65	38	within	within	ADP
ajlm-609	65	39	12	12	NUM
ajlm-609	65	40	h	h	NOUN
ajlm-609	65	41	of	of	ADP
ajlm-609	65	42	collection	collection	NOUN
ajlm-609	65	43	)	)	PUNCT
ajlm-609	65	44	from	from	ADP
ajlm-609	65	45	the	the	DET
ajlm-609	65	46	national	national	PROPN
ajlm-609	65	47	health	health	PROPN
ajlm-609	65	48	laboratory	laboratory	PROPN
ajlm-609	65	49	services	service	NOUN
ajlm-609	65	50	located	locate	VERB
ajlm-609	65	51	at	at	ADP
ajlm-609	65	52	the	the	DET
ajlm-609	65	53	groote	groote	PROPN
ajlm-609	65	54	schuur	schuur	PROPN
ajlm-609	65	55	hospital	hospital	PROPN
ajlm-609	65	56	in	in	ADP
ajlm-609	65	57	cape	cape	NOUN
ajlm-609	65	58	town	town	NOUN
ajlm-609	65	59	,	,	PUNCT
ajlm-609	65	60	south	south	PROPN
ajlm-609	65	61	africa	africa	PROPN
ajlm-609	65	62	,	,	PUNCT
ajlm-609	65	63	a	a	DET
ajlm-609	65	64	tertiary	tertiary	ADJ
ajlm-609	65	65	care	care	NOUN
ajlm-609	65	66	academic	academic	ADJ
ajlm-609	65	67	teaching	teaching	NOUN
ajlm-609	65	68	laboratory	laboratory	NOUN
ajlm-609	65	69	affiliated	affiliate	VERB
ajlm-609	65	70	with	with	ADP
ajlm-609	65	71	the	the	DET
ajlm-609	65	72	university	university	PROPN
ajlm-609	65	73	of	of	ADP
ajlm-609	65	74	cape	cape	NOUN
ajlm-609	65	75	town	town	NOUN
ajlm-609	65	76	.	.	PUNCT
ajlm-609	66	1	this	this	DET
ajlm-609	66	2	tertiary	tertiary	ADJ
ajlm-609	66	3	academic	academic	ADJ
ajlm-609	66	4	hospital	hospital	NOUN
ajlm-609	66	5	serves	serve	VERB
ajlm-609	66	6	the	the	DET
ajlm-609	66	7	greater	great	ADJ
ajlm-609	66	8	cape	cape	NOUN
ajlm-609	66	9	town	town	NOUN
ajlm-609	66	10	area	area	NOUN
ajlm-609	66	11	.	.	PUNCT
ajlm-609	67	1	a	a	DET
ajlm-609	67	2	pea	pea	NOUN
ajlm-609	67	3	-	-	PUNCT
ajlm-609	67	4	sized	sized	ADJ
ajlm-609	67	5	stool	stool	NOUN
ajlm-609	67	6	sample	sample	NOUN
ajlm-609	67	7	was	be	AUX
ajlm-609	67	8	inoculated	inoculate	VERB
ajlm-609	67	9	in	in	ADP
ajlm-609	67	10	90	90	NUM
ajlm-609	67	11	ml	ml	NOUN
ajlm-609	67	12	of	of	ADP
ajlm-609	67	13	tsb	tsb	NOUN
ajlm-609	67	14	and	and	CCONJ
ajlm-609	67	15	vortexed	vortexe	VERB
ajlm-609	67	16	before	before	ADP
ajlm-609	67	17	incubation	incubation	NOUN
ajlm-609	67	18	at	at	ADP
ajlm-609	67	19	37	37	NUM
ajlm-609	67	20	°	°	NOUN
ajlm-609	67	21	c	c	NOUN
ajlm-609	67	22	for	for	ADP
ajlm-609	67	23	18	18	NUM
ajlm-609	67	24	h.	h.	NOUN
ajlm-609	67	25	two	two	NUM
ajlm-609	67	26	hundred	hundred	NUM
ajlm-609	67	27	microlitres	microlitre	NOUN
ajlm-609	67	28	of	of	ADP
ajlm-609	67	29	broth	broth	NOUN
ajlm-609	67	30	were	be	AUX
ajlm-609	67	31	subsequently	subsequently	ADV
ajlm-609	67	32	extracted	extract	VERB
ajlm-609	67	33	employing	employ	VERB
ajlm-609	67	34	the	the	DET
ajlm-609	67	35	magna	magna	PROPN
ajlm-609	67	36	pure	pure	PROPN
ajlm-609	67	37	lc	lc	PROPN
ajlm-609	67	38	total	total	ADJ
ajlm-609	67	39	nucleic	nucleic	ADJ
ajlm-609	67	40	acid	acid	NOUN
ajlm-609	67	41	isolation	isolation	NOUN
ajlm-609	67	42	kit	kit	NOUN
ajlm-609	67	43	(	(	PUNCT
ajlm-609	67	44	roche	roche	NOUN
ajlm-609	67	45	diagnostics	diagnostic	NOUN
ajlm-609	67	46	,	,	PUNCT
ajlm-609	67	47	rotkreuz	rotkreuz	PROPN
ajlm-609	67	48	,	,	PUNCT
ajlm-609	67	49	switzerland	switzerland	PROPN
ajlm-609	67	50	)	)	PUNCT
ajlm-609	67	51	using	use	VERB
ajlm-609	67	52	the	the	DET
ajlm-609	67	53	total	total	ADJ
ajlm-609	67	54	variable	variable	ADJ
ajlm-609	67	55	elution	elution	NOUN
ajlm-609	67	56	volume	volume	NOUN
ajlm-609	67	57	protocol	protocol	NOUN
ajlm-609	67	58	and	and	CCONJ
ajlm-609	67	59	following	follow	VERB
ajlm-609	67	60	the	the	DET
ajlm-609	67	61	manufacturer	manufacturer	NOUN
ajlm-609	67	62	’s	’s	PART
ajlm-609	67	63	manual	manual	NOUN
ajlm-609	67	64	(	(	PUNCT
ajlm-609	67	65	version	version	NOUN
ajlm-609	67	66	14	14	NUM
ajlm-609	67	67	)	)	PUNCT
ajlm-609	67	68	to	to	PART
ajlm-609	67	69	yield	yield	VERB
ajlm-609	67	70	100	100	NUM
ajlm-609	67	71	μl	μl	ADP
ajlm-609	67	72	of	of	ADP
ajlm-609	67	73	nucleic	nucleic	ADJ
ajlm-609	67	74	acid	acid	NOUN
ajlm-609	67	75	extract	extract	NOUN
ajlm-609	67	76	.	.	PUNCT
ajlm-609	68	1	in	in	ADP
ajlm-609	68	2	addition	addition	NOUN
ajlm-609	68	3	,	,	PUNCT
ajlm-609	68	4	chromagartmstec	chromagartmstec	ADJ
ajlm-609	68	5	(	(	PUNCT
ajlm-609	68	6	chromagar	chromagar	ADJ
ajlm-609	68	7	microbiology	microbiology	NOUN
ajlm-609	68	8	,	,	PUNCT
ajlm-609	68	9	paris	paris	PROPN
ajlm-609	68	10	,	,	PUNCT
ajlm-609	68	11	france	france	PROPN
ajlm-609	68	12	)	)	PUNCT
ajlm-609	68	13	was	be	AUX
ajlm-609	68	14	inoculated	inoculate	VERB
ajlm-609	68	15	with	with	ADP
ajlm-609	68	16	a	a	DET
ajlm-609	68	17	loop	loop	NOUN
ajlm-609	68	18	full	full	ADJ
ajlm-609	68	19	of	of	ADP
ajlm-609	68	20	overnight	overnight	ADJ
ajlm-609	68	21	inoculated	inoculate	VERB
ajlm-609	68	22	broth	broth	NOUN
ajlm-609	68	23	and	and	CCONJ
ajlm-609	68	24	incubated	incubate	VERB
ajlm-609	68	25	at	at	ADP
ajlm-609	68	26	37	37	NUM
ajlm-609	68	27	°	°	ADP
ajlm-609	68	28	c	c	NOUN
ajlm-609	68	29	for	for	ADP
ajlm-609	68	30	18	18	NUM
ajlm-609	68	31	h.	h.	NOUN
ajlm-609	68	32	bright	bright	ADJ
ajlm-609	68	33	mauve	mauve	NOUN
ajlm-609	68	34	colonies	colony	NOUN
ajlm-609	68	35	(	(	PUNCT
ajlm-609	68	36	up	up	ADP
ajlm-609	68	37	to	to	PART
ajlm-609	68	38	five	five	NUM
ajlm-609	68	39	mauve	mauve	NOUN
ajlm-609	68	40	colonies	colony	NOUN
ajlm-609	68	41	were	be	AUX
ajlm-609	68	42	picked	pick	VERB
ajlm-609	68	43	per	per	ADP
ajlm-609	68	44	sample	sample	NOUN
ajlm-609	68	45	,	,	PUNCT
ajlm-609	68	46	depending	depend	VERB
ajlm-609	68	47	on	on	ADP
ajlm-609	68	48	the	the	DET
ajlm-609	68	49	number	number	NOUN
ajlm-609	68	50	of	of	ADP
ajlm-609	68	51	mauve	mauve	NOUN
ajlm-609	68	52	colonies	colony	NOUN
ajlm-609	68	53	formed	form	VERB
ajlm-609	68	54	)	)	PUNCT
ajlm-609	68	55	were	be	AUX
ajlm-609	68	56	sub	sub	ADJ
ajlm-609	68	57	-	-	ADJ
ajlm-609	68	58	cultured	cultured	ADJ
ajlm-609	68	59	onto	onto	ADP
ajlm-609	68	60	macconkey	macconkey	NOUN
ajlm-609	68	61	agar	agar	NOUN
ajlm-609	68	62	with	with	ADP
ajlm-609	68	63	crystal	crystal	PROPN
ajlm-609	68	64	violet	violet	NOUN
ajlm-609	68	65	,	,	PUNCT
ajlm-609	68	66	sorbitol	sorbitol	NOUN
ajlm-609	68	67	macconkey	macconkey	NOUN
ajlm-609	68	68	agar	agar	NOUN
ajlm-609	68	69	,	,	PUNCT
ajlm-609	68	70	and	and	CCONJ
ajlm-609	68	71	2	2	NUM
ajlm-609	68	72	%	%	NOUN
ajlm-609	68	73	blood	blood	NOUN
ajlm-609	68	74	agar	agar	NOUN
ajlm-609	68	75	(	(	PUNCT
ajlm-609	68	76	green	green	ADJ
ajlm-609	68	77	point	point	NOUN
ajlm-609	68	78	media	medium	NOUN
ajlm-609	68	79	,	,	PUNCT
ajlm-609	68	80	national	national	PROPN
ajlm-609	68	81	health	health	PROPN
ajlm-609	68	82	laboratory	laboratory	PROPN
ajlm-609	68	83	service	service	PROPN
ajlm-609	68	84	,	,	PUNCT
ajlm-609	68	85	albertynshof	albertynshof	NOUN
ajlm-609	68	86	,	,	PUNCT
ajlm-609	68	87	south	south	PROPN
ajlm-609	68	88	africa	africa	PROPN
ajlm-609	68	89	)	)	PUNCT
ajlm-609	68	90	.	.	PUNCT
ajlm-609	69	1	e.	e.	PROPN
ajlm-609	69	2	coli	coli	PROPN
ajlm-609	69	3	was	be	AUX
ajlm-609	69	4	presumptively	presumptively	ADV
ajlm-609	69	5	identified	identify	VERB
ajlm-609	69	6	as	as	ADP
ajlm-609	69	7	lactose	lactose	NOUN
ajlm-609	69	8	-	-	PUNCT
ajlm-609	69	9	positive	positive	ADJ
ajlm-609	69	10	,	,	PUNCT
ajlm-609	69	11	oxidase	oxidase	NOUN
ajlm-609	69	12	-	-	PUNCT
ajlm-609	69	13	negative	negative	ADJ
ajlm-609	69	14	,	,	PUNCT
ajlm-609	69	15	spot	spot	ADJ
ajlm-609	69	16	indole	indole	NOUN
ajlm-609	69	17	-	-	PUNCT
ajlm-609	69	18	positive	positive	ADJ
ajlm-609	69	19	and	and	CCONJ
ajlm-609	69	20	pyrrolidonyl	pyrrolidonyl	ADJ
ajlm-609	69	21	arylamidase	arylamidase	NOUN
ajlm-609	69	22	(	(	PUNCT
ajlm-609	69	23	pyr)-negative	pyr)-negative	ADJ
ajlm-609	69	24	with	with	ADP
ajlm-609	69	25	confirmatory	confirmatory	NOUN
ajlm-609	69	26	identification	identification	NOUN
ajlm-609	69	27	using	use	VERB
ajlm-609	69	28	vitek	vitek	PROPN
ajlm-609	69	29	2	2	NUM
ajlm-609	69	30	(	(	PUNCT
ajlm-609	69	31	biomerieux	biomerieux	PROPN
ajlm-609	69	32	,	,	PUNCT
ajlm-609	69	33	inc	inc	PROPN
ajlm-609	69	34	.	.	PROPN
ajlm-609	69	35	,	,	PUNCT
ajlm-609	69	36	durham	durham	PROPN
ajlm-609	69	37	,	,	PUNCT
ajlm-609	69	38	north	north	PROPN
ajlm-609	69	39	carolina	carolina	PROPN
ajlm-609	69	40	,	,	PUNCT
ajlm-609	69	41	united	united	PROPN
ajlm-609	69	42	states	states	PROPN
ajlm-609	69	43	)	)	PUNCT
ajlm-609	69	44	.	.	PUNCT
ajlm-609	70	1	isolate	isolate	VERB
ajlm-609	70	2	characterisation	characterisation	NOUN
ajlm-609	70	3	isolates	isolate	NOUN
ajlm-609	70	4	yielding	yield	VERB
ajlm-609	70	5	mauve	mauve	NOUN
ajlm-609	70	6	colonies	colony	NOUN
ajlm-609	70	7	on	on	ADP
ajlm-609	70	8	chromagartmstec	chromagartmstec	ADJ
ajlm-609	70	9	and	and	CCONJ
ajlm-609	70	10	presumptively	presumptively	ADV
ajlm-609	70	11	identified	identify	VERB
ajlm-609	70	12	as	as	ADP
ajlm-609	70	13	e.	e.	PROPN
ajlm-609	70	14	coli	coli	PROPN
ajlm-609	70	15	were	be	AUX
ajlm-609	70	16	subjected	subject	VERB
ajlm-609	70	17	to	to	ADP
ajlm-609	70	18	stx	stx	VERB
ajlm-609	70	19	characterisation	characterisation	NOUN
ajlm-609	70	20	employing	employ	VERB
ajlm-609	70	21	the	the	DET
ajlm-609	70	22	real	real	ADJ
ajlm-609	70	23	-	-	PUNCT
ajlm-609	70	24	time	time	NOUN
ajlm-609	70	25	pcr	pcr	PROPN
ajlm-609	70	26	assay	assay	NOUN
ajlm-609	70	27	characterised	characterise	VERB
ajlm-609	70	28	herein	herein	NOUN
ajlm-609	70	29	.	.	PUNCT
ajlm-609	71	1	other	other	ADJ
ajlm-609	71	2	diarrhoeic	diarrhoeic	VERB
ajlm-609	71	3	e.	e.	PROPN
ajlm-609	71	4	coli	coli	PROPN
ajlm-609	71	5	virulence	virulence	NOUN
ajlm-609	71	6	genes	gene	NOUN
ajlm-609	71	7	,	,	PUNCT
ajlm-609	71	8	including	include	VERB
ajlm-609	71	9	the	the	DET
ajlm-609	71	10	fimbrial	fimbrial	ADJ
ajlm-609	71	11	adhesion	adhesion	NOUN
ajlm-609	71	12	gene	gene	NOUN
ajlm-609	71	13	for	for	ADP
ajlm-609	71	14	diffusely	diffusely	ADV
ajlm-609	71	15	adherent	adherent	ADJ
ajlm-609	71	16	e.	e.	PROPN
ajlm-609	71	17	coli	coli	PROPN
ajlm-609	71	18	,	,	PUNCT
ajlm-609	71	19	the	the	DET
ajlm-609	71	20	anti	anti	ADJ
ajlm-609	71	21	-	-	ADJ
ajlm-609	71	22	aggregation	aggregation	ADJ
ajlm-609	71	23	protein	protein	NOUN
ajlm-609	71	24	transporter	transporter	NOUN
ajlm-609	71	25	gene	gene	NOUN
ajlm-609	71	26	for	for	ADP
ajlm-609	71	27	enteroaggregative	enteroaggregative	ADJ
ajlm-609	71	28	e.	e.	PROPN
ajlm-609	71	29	coli	coli	PROPN
ajlm-609	71	30	,	,	PUNCT
ajlm-609	71	31	heat	heat	NOUN
ajlm-609	71	32	-	-	PUNCT
ajlm-609	71	33	stable	stable	ADJ
ajlm-609	71	34	and	and	CCONJ
ajlm-609	71	35	heat	heat	NOUN
ajlm-609	71	36	-	-	PUNCT
ajlm-609	71	37	labile	labile	NOUN
ajlm-609	71	38	enterotoxin	enterotoxin	NOUN
ajlm-609	71	39	genes	gene	NOUN
ajlm-609	71	40	of	of	ADP
ajlm-609	71	41	enterotoxigenic	enterotoxigenic	ADJ
ajlm-609	71	42	e.	e.	PROPN
ajlm-609	71	43	coli	coli	PROPN
ajlm-609	71	44	,	,	PUNCT
ajlm-609	71	45	the	the	DET
ajlm-609	71	46	intimin	intimin	NOUN
ajlm-609	71	47	coding	code	VERB
ajlm-609	71	48	gene	gene	NOUN
ajlm-609	71	49	eae	eae	PROPN
ajlm-609	71	50	for	for	ADP
ajlm-609	71	51	enteropathogenic	enteropathogenic	ADJ
ajlm-609	71	52	e.	e.	PROPN
ajlm-609	71	53	coli	coli	PROPN
ajlm-609	71	54	(	(	PUNCT
ajlm-609	71	55	epec	epec	NOUN
ajlm-609	71	56	)	)	PUNCT
ajlm-609	71	57	and	and	CCONJ
ajlm-609	71	58	the	the	DET
ajlm-609	71	59	bundle	bundle	NOUN
ajlm-609	71	60	-	-	PUNCT
ajlm-609	71	61	forming	form	VERB
ajlm-609	71	62	pili	pili	NOUN
ajlm-609	71	63	gene	gene	NOUN
ajlm-609	71	64	for	for	ADP
ajlm-609	71	65	the	the	DET
ajlm-609	71	66	typical	typical	ADJ
ajlm-609	71	67	epec	epec	NOUN
ajlm-609	71	68	,	,	PUNCT
ajlm-609	71	69	were	be	AUX
ajlm-609	71	70	determined	determine	VERB
ajlm-609	71	71	using	use	VERB
ajlm-609	71	72	standard	standard	ADJ
ajlm-609	71	73	gel	gel	NOUN
ajlm-609	71	74	-	-	PUNCT
ajlm-609	71	75	based	base	VERB
ajlm-609	71	76	pcr	pcr	NOUN
ajlm-609	71	77	as	as	SCONJ
ajlm-609	71	78	previously	previously	ADV
ajlm-609	71	79	described	describe	VERB
ajlm-609	71	80	using	use	VERB
ajlm-609	71	81	primers	primer	NOUN
ajlm-609	71	82	as	as	SCONJ
ajlm-609	71	83	shown	show	VERB
ajlm-609	71	84	in	in	ADP
ajlm-609	71	85	table	table	NOUN
ajlm-609	71	86	2.18	2.18	NUM
ajlm-609	71	87	table	table	NOUN
ajlm-609	71	88	2	2	NUM
ajlm-609	71	89	:	:	PUNCT
ajlm-609	71	90	 	 	SPACE
ajlm-609	71	91	primers	primer	NOUN
ajlm-609	71	92	used	use	VERB
ajlm-609	71	93	for	for	ADP
ajlm-609	71	94	detection	detection	NOUN
ajlm-609	71	95	of	of	ADP
ajlm-609	71	96	virulence	virulence	NOUN
ajlm-609	71	97	genes	gene	NOUN
ajlm-609	71	98	by	by	ADP
ajlm-609	71	99	conventional	conventional	ADJ
ajlm-609	71	100	polymerase	polymerase	NOUN
ajlm-609	71	101	chain	chain	NOUN
ajlm-609	71	102	reaction	reaction	NOUN
ajlm-609	71	103	.	.	PUNCT
ajlm-609	72	1	to	to	PART
ajlm-609	72	2	confirm	confirm	VERB
ajlm-609	72	3	shiga	shiga	ADJ
ajlm-609	72	4	toxin	toxin	NOUN
ajlm-609	72	5	production	production	NOUN
ajlm-609	72	6	among	among	ADP
ajlm-609	72	7	stx	stx	ADJ
ajlm-609	72	8	-	-	ADJ
ajlm-609	72	9	positive	positive	ADJ
ajlm-609	72	10	isolates	isolate	NOUN
ajlm-609	72	11	,	,	PUNCT
ajlm-609	72	12	the	the	DET
ajlm-609	72	13	immunocard	immunocard	ADJ
ajlm-609	72	14	stat	stat	PROPN
ajlm-609	72	15	!	!	PUNCT
ajlm-609	73	1	®	®	NOUN
ajlm-609	73	2	ehec	ehec	PROPN
ajlm-609	73	3	(	(	PUNCT
ajlm-609	73	4	meridian	meridian	PROPN
ajlm-609	73	5	biosciences	biosciences	PROPN
ajlm-609	73	6	,	,	PUNCT
ajlm-609	73	7	inc	inc	PROPN
ajlm-609	73	8	.	.	PROPN
ajlm-609	73	9	,	,	PUNCT
ajlm-609	73	10	cincinnati	cincinnati	PROPN
ajlm-609	73	11	,	,	PUNCT
ajlm-609	73	12	ohio	ohio	PROPN
ajlm-609	73	13	,	,	PUNCT
ajlm-609	73	14	united	united	PROPN
ajlm-609	73	15	states	states	PROPN
ajlm-609	73	16	)	)	PUNCT
ajlm-609	73	17	was	be	AUX
ajlm-609	73	18	used	use	VERB
ajlm-609	73	19	to	to	PART
ajlm-609	73	20	detect	detect	VERB
ajlm-609	73	21	shiga	shiga	ADJ
ajlm-609	73	22	toxin	toxin	NOUN
ajlm-609	73	23	1	1	NUM
ajlm-609	73	24	and	and	CCONJ
ajlm-609	73	25	2	2	NUM
ajlm-609	73	26	(	(	PUNCT
ajlm-609	73	27	by	by	ADP
ajlm-609	73	28	employing	employ	VERB
ajlm-609	73	29	immunochromatography	immunochromatography	NOUN
ajlm-609	73	30	with	with	ADP
ajlm-609	73	31	toxin	toxin	NOUN
ajlm-609	73	32	-	-	PUNCT
ajlm-609	73	33	directed	direct	VERB
ajlm-609	73	34	monoclonal	monoclonal	NOUN
ajlm-609	73	35	antibodies	antibody	NOUN
ajlm-609	73	36	labelled	label	VERB
ajlm-609	73	37	with	with	ADP
ajlm-609	73	38	red	red	ADJ
ajlm-609	73	39	-	-	PUNCT
ajlm-609	73	40	coloured	coloured	ADJ
ajlm-609	73	41	gold	gold	NOUN
ajlm-609	73	42	particles	particle	NOUN
ajlm-609	73	43	)	)	PUNCT
ajlm-609	73	44	.	.	PUNCT
ajlm-609	74	1	all	all	DET
ajlm-609	74	2	mauve	mauve	NOUN
ajlm-609	74	3	isolates	isolate	NOUN
ajlm-609	74	4	found	find	VERB
ajlm-609	74	5	to	to	PART
ajlm-609	74	6	carry	carry	VERB
ajlm-609	74	7	virulence	virulence	NOUN
ajlm-609	74	8	genes	gene	NOUN
ajlm-609	74	9	were	be	AUX
ajlm-609	74	10	serotyped	serotype	VERB
ajlm-609	74	11	at	at	ADP
ajlm-609	74	12	the	the	DET
ajlm-609	74	13	centre	centre	NOUN
ajlm-609	74	14	for	for	ADP
ajlm-609	74	15	enteric	enteric	ADJ
ajlm-609	74	16	diseases	disease	NOUN
ajlm-609	74	17	,	,	PUNCT
ajlm-609	74	18	national	national	PROPN
ajlm-609	74	19	institute	institute	PROPN
ajlm-609	74	20	of	of	ADP
ajlm-609	74	21	communicable	communicable	NOUN
ajlm-609	74	22	disease	disease	NOUN
ajlm-609	74	23	,	,	PUNCT
ajlm-609	74	24	johannesburg	johannesburg	PROPN
ajlm-609	74	25	,	,	PUNCT
ajlm-609	74	26	by	by	ADP
ajlm-609	74	27	employing	employ	VERB
ajlm-609	74	28	antisera	antisera	NOUN
ajlm-609	74	29	(	(	PUNCT
ajlm-609	74	30	statens	staten	NOUN
ajlm-609	74	31	serum	serum	PROPN
ajlm-609	74	32	institut	institut	PROPN
ajlm-609	74	33	,	,	PUNCT
ajlm-609	74	34	copenhagen	copenhagen	PROPN
ajlm-609	74	35	,	,	PUNCT
ajlm-609	74	36	denmark	denmark	PROPN
ajlm-609	74	37	)	)	PUNCT
ajlm-609	74	38	and	and	CCONJ
ajlm-609	74	39	the	the	DET
ajlm-609	74	40	detection	detection	NOUN
ajlm-609	74	41	of	of	ADP
ajlm-609	74	42	somatic	somatic	ADJ
ajlm-609	74	43	o	o	NOUN
ajlm-609	74	44	-	-	NOUN
ajlm-609	74	45	antigens	antigen	NOUN
ajlm-609	74	46	as	as	ADP
ajlm-609	74	47	previously	previously	ADV
ajlm-609	74	48	described.19,20	described.19,20	ADJ
ajlm-609	74	49	h	h	ADJ
ajlm-609	74	50	-	-	PUNCT
ajlm-609	74	51	antigen	antigen	ADJ
ajlm-609	74	52	serotyping	serotyping	NOUN
ajlm-609	74	53	was	be	AUX
ajlm-609	74	54	not	not	PART
ajlm-609	74	55	undertaken	undertake	VERB
ajlm-609	74	56	.	.	PUNCT
ajlm-609	75	1	statistical	statistical	ADJ
ajlm-609	75	2	analysis	analysis	NOUN
ajlm-609	75	3	data	datum	NOUN
ajlm-609	75	4	on	on	ADP
ajlm-609	75	5	the	the	DET
ajlm-609	75	6	possession	possession	NOUN
ajlm-609	75	7	of	of	ADP
ajlm-609	75	8	virulence	virulence	NOUN
ajlm-609	75	9	genes	gene	NOUN
ajlm-609	75	10	,	,	PUNCT
ajlm-609	75	11	cultural	cultural	ADJ
ajlm-609	75	12	characteristics	characteristic	NOUN
ajlm-609	75	13	on	on	ADP
ajlm-609	75	14	different	different	ADJ
ajlm-609	75	15	media	medium	NOUN
ajlm-609	75	16	,	,	PUNCT
ajlm-609	75	17	serotypes	serotype	NOUN
ajlm-609	75	18	and	and	CCONJ
ajlm-609	75	19	shiga	shiga	ADJ
ajlm-609	75	20	toxin	toxin	NOUN
ajlm-609	75	21	production	production	NOUN
ajlm-609	75	22	was	be	AUX
ajlm-609	75	23	entered	enter	VERB
ajlm-609	75	24	in	in	ADP
ajlm-609	75	25	microsoft	microsoft	PROPN
ajlm-609	75	26	office	office	PROPN
ajlm-609	75	27	excel	excel	VERB
ajlm-609	75	28	2010	2010	NUM
ajlm-609	75	29	(	(	PUNCT
ajlm-609	75	30	microsoft	microsoft	PROPN
ajlm-609	75	31	corp	corp	PROPN
ajlm-609	75	32	.	.	PROPN
ajlm-609	75	33	,redmond	,redmond	PROPN
ajlm-609	75	34	,	,	PUNCT
ajlm-609	75	35	washington	washington	PROPN
ajlm-609	75	36	,	,	PUNCT
ajlm-609	75	37	united	united	PROPN
ajlm-609	75	38	states	states	PROPN
ajlm-609	75	39	)	)	PUNCT
ajlm-609	75	40	and	and	CCONJ
ajlm-609	75	41	then	then	ADV
ajlm-609	75	42	exported	export	VERB
ajlm-609	75	43	to	to	ADP
ajlm-609	75	44	epiinfotm	epiinfotm	PROPN
ajlm-609	75	45	7.1.5.2	7.1.5.2	PROPN
ajlm-609	75	46	(	(	PUNCT
ajlm-609	75	47	centers	center	NOUN
ajlm-609	75	48	for	for	ADP
ajlm-609	75	49	disease	disease	NOUN
ajlm-609	75	50	control	control	NOUN
ajlm-609	75	51	and	and	CCONJ
ajlm-609	75	52	prevention	prevention	NOUN
ajlm-609	75	53	,	,	PUNCT
ajlm-609	75	54	atlanta	atlanta	PROPN
ajlm-609	75	55	,	,	PUNCT
ajlm-609	75	56	georgia	georgia	PROPN
ajlm-609	75	57	,	,	PUNCT
ajlm-609	75	58	united	united	PROPN
ajlm-609	75	59	states	states	PROPN
ajlm-609	75	60	)	)	PUNCT
ajlm-609	75	61	for	for	ADP
ajlm-609	75	62	analysis	analysis	NOUN
ajlm-609	75	63	.	.	PUNCT
ajlm-609	76	1	using	use	VERB
ajlm-609	76	2	the	the	DET
ajlm-609	76	3	lightcycler	lightcycler	NOUN
ajlm-609	76	4	480	480	NUM
ajlm-609	76	5	ii	ii	PROPN
ajlm-609	76	6	software	software	NOUN
ajlm-609	76	7	,	,	PUNCT
ajlm-609	76	8	efficiency	efficiency	NOUN
ajlm-609	76	9	of	of	ADP
ajlm-609	76	10	the	the	DET
ajlm-609	76	11	in	in	ADP
ajlm-609	76	12	-	-	PUNCT
ajlm-609	76	13	house	house	NOUN
ajlm-609	76	14	real	real	ADJ
ajlm-609	76	15	-	-	PUNCT
ajlm-609	76	16	time	time	NOUN
ajlm-609	76	17	pcr	pcr	PROPN
ajlm-609	76	18	assay	assay	NOUN
ajlm-609	76	19	was	be	AUX
ajlm-609	76	20	determined	determine	VERB
ajlm-609	76	21	.	.	PUNCT
ajlm-609	77	1	the	the	DET
ajlm-609	77	2	amplification	amplification	NOUN
ajlm-609	77	3	curves	curve	NOUN
ajlm-609	77	4	and	and	CCONJ
ajlm-609	77	5	the	the	DET
ajlm-609	77	6	melting	melting	NOUN
ajlm-609	77	7	peaks	peak	NOUN
ajlm-609	77	8	were	be	AUX
ajlm-609	77	9	used	use	VERB
ajlm-609	77	10	to	to	PART
ajlm-609	77	11	differentiate	differentiate	VERB
ajlm-609	77	12	between	between	ADP
ajlm-609	77	13	stx1	stx1	NOUN
ajlm-609	77	14	and	and	CCONJ
ajlm-609	77	15	stx2	stx2	PROPN
ajlm-609	77	16	.	.	PUNCT
ajlm-609	78	1	results	result	VERB
ajlm-609	78	2	real	real	ADJ
ajlm-609	78	3	-	-	PUNCT
ajlm-609	78	4	time	time	NOUN
ajlm-609	78	5	polymerase	polymerase	NOUN
ajlm-609	78	6	chain	chain	NOUN
ajlm-609	78	7	reaction	reaction	NOUN
ajlm-609	78	8	validation	validation	NOUN
ajlm-609	78	9	the	the	DET
ajlm-609	78	10	blast	blast	NOUN
ajlm-609	78	11	analysis	analysis	NOUN
ajlm-609	78	12	of	of	ADP
ajlm-609	78	13	the	the	DET
ajlm-609	78	14	primers	primer	NOUN
ajlm-609	78	15	and	and	CCONJ
ajlm-609	78	16	probe	probe	NOUN
ajlm-609	78	17	sequence	sequence	NOUN
ajlm-609	78	18	specificity	specificity	NOUN
ajlm-609	78	19	yielded	yield	VERB
ajlm-609	78	20	no	no	DET
ajlm-609	78	21	significant	significant	ADJ
ajlm-609	78	22	homology	homology	NOUN
ajlm-609	78	23	to	to	ADP
ajlm-609	78	24	non	non	ADJ
ajlm-609	78	25	-	-	ADJ
ajlm-609	78	26	stx	stx	ADJ
ajlm-609	78	27	targets	target	NOUN
ajlm-609	78	28	(	(	PUNCT
ajlm-609	78	29	data	datum	NOUN
ajlm-609	78	30	not	not	PART
ajlm-609	78	31	shown	show	VERB
ajlm-609	78	32	)	)	PUNCT
ajlm-609	78	33	.	.	PUNCT
ajlm-609	79	1	real	real	ADJ
ajlm-609	79	2	-	-	PUNCT
ajlm-609	79	3	time	time	NOUN
ajlm-609	79	4	pcr	pcr	NOUN
ajlm-609	79	5	amplicons	amplicon	NOUN
ajlm-609	79	6	generated	generate	VERB
ajlm-609	79	7	were	be	AUX
ajlm-609	79	8	confirmed	confirm	VERB
ajlm-609	79	9	as	as	ADP
ajlm-609	79	10	208	208	NUM
ajlm-609	79	11	bp	bp	NOUN
ajlm-609	79	12	for	for	ADP
ajlm-609	79	13	stx1	stx1	NOUN
ajlm-609	79	14	and	and	CCONJ
ajlm-609	79	15	204	204	NUM
ajlm-609	79	16	bp	bp	NOUN
ajlm-609	79	17	for	for	ADP
ajlm-609	79	18	stx2	stx2	PROPN
ajlm-609	79	19	(	(	PUNCT
ajlm-609	79	20	figure	figure	NOUN
ajlm-609	79	21	2	2	NUM
ajlm-609	79	22	)	)	PUNCT
ajlm-609	79	23	.	.	PUNCT
ajlm-609	80	1	sequencing	sequence	VERB
ajlm-609	80	2	and	and	CCONJ
ajlm-609	80	3	blast	blast	NOUN
ajlm-609	80	4	analysis	analysis	NOUN
ajlm-609	80	5	confirmed	confirm	VERB
ajlm-609	80	6	the	the	DET
ajlm-609	80	7	identity	identity	NOUN
ajlm-609	80	8	of	of	ADP
ajlm-609	80	9	both	both	CCONJ
ajlm-609	80	10	stx1	stx1	NOUN
ajlm-609	80	11	and	and	CCONJ
ajlm-609	80	12	stx2	stx2	ADJ
ajlm-609	80	13	amplicons	amplicon	NOUN
ajlm-609	80	14	.	.	PUNCT
ajlm-609	81	1	the	the	DET
ajlm-609	81	2	serially	serially	ADV
ajlm-609	81	3	diluted	dilute	VERB
ajlm-609	81	4	plasmid	plasmid	NOUN
ajlm-609	81	5	-	-	PUNCT
ajlm-609	81	6	stool	stool	NOUN
ajlm-609	81	7	-	-	PUNCT
ajlm-609	81	8	tsb	tsb	PROPN
ajlm-609	81	9	was	be	AUX
ajlm-609	81	10	successfully	successfully	ADV
ajlm-609	81	11	amplified	amplify	VERB
ajlm-609	81	12	in	in	ADP
ajlm-609	81	13	8/8	8/8	NUM
ajlm-609	81	14	replicates	replicate	NOUN
ajlm-609	81	15	in	in	ADP
ajlm-609	81	16	the	the	DET
ajlm-609	81	17	sixth	sixth	ADJ
ajlm-609	81	18	dilution	dilution	NOUN
ajlm-609	81	19	,	,	PUNCT
ajlm-609	81	20	whereas	whereas	SCONJ
ajlm-609	81	21	the	the	DET
ajlm-609	81	22	seventh	seventh	ADJ
ajlm-609	81	23	dilution	dilution	NOUN
ajlm-609	81	24	yielded	yield	VERB
ajlm-609	81	25	an	an	DET
ajlm-609	81	26	amplification	amplification	NOUN
ajlm-609	81	27	signal	signal	NOUN
ajlm-609	81	28	in	in	ADP
ajlm-609	81	29	3/8	3/8	NUM
ajlm-609	81	30	replicates	replicate	NOUN
ajlm-609	81	31	,	,	PUNCT
ajlm-609	81	32	yielding	yield	VERB
ajlm-609	81	33	a	a	DET
ajlm-609	81	34	lod	lod	NOUN
ajlm-609	81	35	of	of	ADP
ajlm-609	81	36	5.3	5.3	NUM
ajlm-609	81	37	target	target	NOUN
ajlm-609	81	38	copies	copy	NOUN
ajlm-609	81	39	/	/	SYM
ajlm-609	81	40	μl	μl	NUM
ajlm-609	81	41	of	of	ADP
ajlm-609	81	42	broth	broth	NOUN
ajlm-609	81	43	.	.	PUNCT
ajlm-609	82	1	all	all	DET
ajlm-609	82	2	other	other	ADJ
ajlm-609	82	3	stx	stx	ADJ
ajlm-609	82	4	subtypes	subtype	NOUN
ajlm-609	82	5	investigated	investigate	VERB
ajlm-609	82	6	(	(	PUNCT
ajlm-609	82	7	stx1a	stx1a	ADV
ajlm-609	82	8	,	,	PUNCT
ajlm-609	82	9	stx1b	stx1b	PROPN
ajlm-609	82	10	,	,	PUNCT
ajlm-609	82	11	stx2a	stx2a	PROPN
ajlm-609	82	12	,	,	PUNCT
ajlm-609	82	13	stx2b	stx2b	PROPN
ajlm-609	82	14	,	,	PUNCT
ajlm-609	82	15	stx2c	stx2c	PROPN
ajlm-609	82	16	,	,	PUNCT
ajlm-609	82	17	stx2d	stx2d	PROPN
ajlm-609	82	18	,	,	PUNCT
ajlm-609	82	19	stx2e	stx2e	PROPN
ajlm-609	82	20	,	,	PUNCT
ajlm-609	82	21	stx2f	stx2f	ADJ
ajlm-609	82	22	,	,	PUNCT
ajlm-609	82	23	and	and	CCONJ
ajlm-609	82	24	stx2	stx2	PROPN
ajlm-609	82	25	g	g	NOUN
ajlm-609	82	26	)	)	PUNCT
ajlm-609	82	27	were	be	AUX
ajlm-609	82	28	successfully	successfully	ADV
ajlm-609	82	29	amplified	amplify	VERB
ajlm-609	82	30	by	by	ADP
ajlm-609	82	31	this	this	DET
ajlm-609	82	32	assay	assay	NOUN
ajlm-609	82	33	(	(	PUNCT
ajlm-609	82	34	data	datum	NOUN
ajlm-609	82	35	not	not	PART
ajlm-609	82	36	shown	show	VERB
ajlm-609	82	37	)	)	PUNCT
ajlm-609	82	38	.	.	PUNCT
ajlm-609	83	1	stx1	stx1	NOUN
ajlm-609	83	2	and	and	CCONJ
ajlm-609	83	3	stx2	stx2	PROPN
ajlm-609	83	4	were	be	AUX
ajlm-609	83	5	successfully	successfully	ADV
ajlm-609	83	6	distinguished	distinguish	VERB
ajlm-609	83	7	by	by	ADP
ajlm-609	83	8	a	a	DET
ajlm-609	83	9	melting	melt	VERB
ajlm-609	83	10	temperature	temperature	NOUN
ajlm-609	83	11	of	of	ADP
ajlm-609	83	12	58.2	58.2	NUM
ajlm-609	83	13	°	°	NOUN
ajlm-609	83	14	c	c	NOUN
ajlm-609	83	15	(	(	PUNCT
ajlm-609	83	16	sd	sd	NOUN
ajlm-609	83	17	=	=	SYM
ajlm-609	83	18	0.033	0.033	NUM
ajlm-609	83	19	)	)	PUNCT
ajlm-609	83	20	and	and	CCONJ
ajlm-609	83	21	65.3	65.3	NUM
ajlm-609	83	22	°	°	NOUN
ajlm-609	83	23	c	c	NOUN
ajlm-609	83	24	(	(	PUNCT
ajlm-609	83	25	sd	sd	NOUN
ajlm-609	83	26	=	=	NOUN
ajlm-609	83	27	0.037	0.037	NUM
ajlm-609	83	28	)	)	PUNCT
ajlm-609	83	29	(	(	PUNCT
ajlm-609	83	30	figures	figure	NOUN
ajlm-609	83	31	3–6	3–6	NUM
ajlm-609	83	32	)	)	PUNCT
ajlm-609	83	33	.	.	PUNCT
ajlm-609	84	1	the	the	DET
ajlm-609	84	2	tm	tm	PROPN
ajlm-609	84	3	for	for	ADP
ajlm-609	84	4	stx2	stx2	ADJ
ajlm-609	84	5	subtypes	subtype	NOUN
ajlm-609	84	6	2a	2a	NUM
ajlm-609	84	7	,	,	PUNCT
ajlm-609	84	8	2b	2b	NOUN
ajlm-609	84	9	,	,	PUNCT
ajlm-609	84	10	2c	2c	NUM
ajlm-609	84	11	,	,	PUNCT
ajlm-609	84	12	2d	2d	NOUN
ajlm-609	84	13	,	,	PUNCT
ajlm-609	84	14	2e	2e	NUM
ajlm-609	84	15	,	,	PUNCT
ajlm-609	84	16	2f	2f	NUM
ajlm-609	84	17	and	and	CCONJ
ajlm-609	84	18	2	2	NUM
ajlm-609	84	19	g	g	NOUN
ajlm-609	84	20	were	be	AUX
ajlm-609	84	21	the	the	DET
ajlm-609	84	22	same	same	ADJ
ajlm-609	84	23	at	at	ADP
ajlm-609	84	24	65.3	65.3	NUM
ajlm-609	84	25	°	°	ADP
ajlm-609	84	26	c	c	NOUN
ajlm-609	84	27	(	(	PUNCT
ajlm-609	84	28	sd	sd	NOUN
ajlm-609	84	29	=	=	NOUN
ajlm-609	84	30	0.037	0.037	NUM
ajlm-609	84	31	,	,	PUNCT
ajlm-609	84	32	0.041	0.041	NUM
ajlm-609	84	33	,	,	PUNCT
ajlm-609	84	34	0.035	0.035	NUM
ajlm-609	84	35	,	,	PUNCT
ajlm-609	84	36	0.039	0.039	NUM
ajlm-609	84	37	,	,	PUNCT
ajlm-609	84	38	0.034	0.034	NUM
ajlm-609	84	39	,	,	PUNCT
ajlm-609	84	40	0.033	0.033	NUM
ajlm-609	84	41	and	and	CCONJ
ajlm-609	84	42	0.032	0.032	NUM
ajlm-609	84	43	,	,	PUNCT
ajlm-609	84	44	respectively	respectively	ADV
ajlm-609	84	45	)	)	PUNCT
ajlm-609	84	46	,	,	PUNCT
ajlm-609	84	47	whereas	whereas	SCONJ
ajlm-609	84	48	that	that	PRON
ajlm-609	84	49	of	of	ADP
ajlm-609	84	50	1d	1d	NUM
ajlm-609	84	51	was	be	AUX
ajlm-609	84	52	44.7	44.7	NUM
ajlm-609	84	53	°	°	NOUN
ajlm-609	84	54	c	c	NOUN
ajlm-609	84	55	(	(	PUNCT
ajlm-609	84	56	sd	sd	NOUN
ajlm-609	84	57	=	=	NUM
ajlm-609	84	58	0.042	0.042	NUM
ajlm-609	84	59	)	)	PUNCT
ajlm-609	84	60	.	.	PUNCT
ajlm-609	85	1	the	the	DET
ajlm-609	85	2	efficiency	efficiency	NOUN
ajlm-609	85	3	of	of	ADP
ajlm-609	85	4	the	the	DET
ajlm-609	85	5	assay	assay	NOUN
ajlm-609	85	6	was	be	AUX
ajlm-609	85	7	1.99	1.99	NUM
ajlm-609	85	8	as	as	SCONJ
ajlm-609	85	9	calculated	calculate	VERB
ajlm-609	85	10	from	from	ADP
ajlm-609	85	11	the	the	DET
ajlm-609	85	12	amplification	amplification	NOUN
ajlm-609	85	13	curves	curve	NOUN
ajlm-609	85	14	generated	generate	VERB
ajlm-609	85	15	using	use	VERB
ajlm-609	85	16	the	the	DET
ajlm-609	85	17	light	light	ADJ
ajlm-609	85	18	cycler	cycler	NOUN
ajlm-609	85	19	®	®	PROPN
ajlm-609	85	20	480	480	NUM
ajlm-609	85	21	software	software	NOUN
ajlm-609	85	22	.	.	PUNCT
ajlm-609	86	1	the	the	DET
ajlm-609	86	2	duplex	duplex	NOUN
ajlm-609	86	3	assay	assay	NOUN
ajlm-609	86	4	detected	detect	VERB
ajlm-609	86	5	both	both	DET
ajlm-609	86	6	targets	target	NOUN
ajlm-609	86	7	in	in	ADP
ajlm-609	86	8	the	the	DET
ajlm-609	86	9	same	same	ADJ
ajlm-609	86	10	run	run	NOUN
ajlm-609	86	11	,	,	PUNCT
ajlm-609	86	12	and	and	CCONJ
ajlm-609	86	13	these	these	PRON
ajlm-609	86	14	could	could	AUX
ajlm-609	86	15	be	be	AUX
ajlm-609	86	16	differentiated	differentiate	VERB
ajlm-609	86	17	by	by	ADP
ajlm-609	86	18	the	the	DET
ajlm-609	86	19	melt	melt	NOUN
ajlm-609	86	20	curve	curve	NOUN
ajlm-609	86	21	with	with	ADP
ajlm-609	86	22	two	two	NUM
ajlm-609	86	23	distinct	distinct	ADJ
ajlm-609	86	24	peaks	peak	NOUN
ajlm-609	86	25	at	at	ADP
ajlm-609	86	26	58.2	58.2	NUM
ajlm-609	86	27	°	°	ADP
ajlm-609	86	28	c	c	NOUN
ajlm-609	86	29	for	for	ADP
ajlm-609	86	30	stx1	stx1	NOUN
ajlm-609	86	31	and	and	CCONJ
ajlm-609	86	32	65.3	65.3	NUM
ajlm-609	86	33	°	°	NOUN
ajlm-609	86	34	c	c	NOUN
ajlm-609	86	35	for	for	ADP
ajlm-609	86	36	stx2	stx2	NOUN
ajlm-609	86	37	(	(	PUNCT
ajlm-609	86	38	figure	figure	VERB
ajlm-609	86	39	7	7	NUM
ajlm-609	86	40	)	)	PUNCT
ajlm-609	86	41	.	.	PUNCT
ajlm-609	87	1	figure	figure	VERB
ajlm-609	87	2	2	2	NUM
ajlm-609	87	3	:	:	PUNCT
ajlm-609	87	4	polymerase	polymerase	NOUN
ajlm-609	87	5	chain	chain	NOUN
ajlm-609	87	6	reaction	reaction	NOUN
ajlm-609	87	7	amplicons	amplicon	NOUN
ajlm-609	87	8	of	of	ADP
ajlm-609	87	9	stx1	stx1	NOUN
ajlm-609	87	10	and	and	CCONJ
ajlm-609	87	11	stx2	stx2	VERB
ajlm-609	87	12	after	after	ADP
ajlm-609	87	13	cloning	clone	VERB
ajlm-609	87	14	.	.	PUNCT
ajlm-609	88	1	figure	figure	VERB
ajlm-609	88	2	3	3	NUM
ajlm-609	88	3	:	:	PUNCT
ajlm-609	88	4	amplification	amplification	NOUN
ajlm-609	88	5	curves	curve	NOUN
ajlm-609	88	6	for	for	ADP
ajlm-609	88	7	the	the	DET
ajlm-609	88	8	validated	validate	VERB
ajlm-609	88	9	duplex	duplex	NOUN
ajlm-609	88	10	real	real	ADJ
ajlm-609	88	11	-	-	PUNCT
ajlm-609	88	12	time	time	NOUN
ajlm-609	88	13	polymerase	polymerase	NOUN
ajlm-609	88	14	chain	chain	NOUN
ajlm-609	88	15	reaction	reaction	NOUN
ajlm-609	88	16	for	for	ADP
ajlm-609	88	17	detection	detection	NOUN
ajlm-609	88	18	of	of	ADP
ajlm-609	88	19	the	the	DET
ajlm-609	88	20	stx	stx	NOUN
ajlm-609	88	21	gene	gene	NOUN
ajlm-609	88	22	figure	figure	NOUN
ajlm-609	88	23	4	4	NUM
ajlm-609	88	24	:	:	PUNCT
ajlm-609	88	25	melting	melt	VERB
ajlm-609	88	26	peaks	peak	NOUN
ajlm-609	88	27	for	for	ADP
ajlm-609	88	28	stx1	stx1	NOUN
ajlm-609	88	29	–	–	PUNCT
ajlm-609	88	30	peak	peak	VERB
ajlm-609	88	31	a	a	DET
ajlm-609	88	32	(	(	PUNCT
ajlm-609	88	33	subtypes	subtype	NOUN
ajlm-609	88	34	other	other	ADJ
ajlm-609	88	35	than	than	ADP
ajlm-609	88	36	1d	1d	NUM
ajlm-609	88	37	–	–	PUNCT
ajlm-609	88	38	55.7	55.7	NUM
ajlm-609	88	39	°	°	NOUN
ajlm-609	88	40	c	c	NOUN
ajlm-609	88	41	)	)	PUNCT
ajlm-609	88	42	and	and	CCONJ
ajlm-609	88	43	stx2	stx2	NOUN
ajlm-609	88	44	–	–	PUNCT
ajlm-609	88	45	peak	peak	NOUN
ajlm-609	88	46	b	b	SYM
ajlm-609	88	47	(	(	PUNCT
ajlm-609	88	48	65.3	65.3	NUM
ajlm-609	88	49	°	°	NOUN
ajlm-609	88	50	c	c	NOUN
ajlm-609	88	51	)	)	PUNCT
ajlm-609	88	52	detected	detect	VERB
ajlm-609	88	53	in	in	ADP
ajlm-609	88	54	the	the	DET
ajlm-609	88	55	same	same	ADJ
ajlm-609	88	56	run	run	NOUN
ajlm-609	88	57	.	.	PUNCT
ajlm-609	89	1	figure	figure	NOUN
ajlm-609	89	2	5	5	NUM
ajlm-609	89	3	:	:	PUNCT
ajlm-609	89	4	melting	melt	VERB
ajlm-609	89	5	peaks	peak	NOUN
ajlm-609	89	6	for	for	ADP
ajlm-609	89	7	optimised	optimise	VERB
ajlm-609	89	8	duplex	duplex	NOUN
ajlm-609	89	9	real	real	ADJ
ajlm-609	89	10	-	-	PUNCT
ajlm-609	89	11	time	time	NOUN
ajlm-609	89	12	polymerase	polymerase	NOUN
ajlm-609	89	13	chain	chain	NOUN
ajlm-609	89	14	reaction	reaction	NOUN
ajlm-609	89	15	showing	show	VERB
ajlm-609	89	16	stx2	stx2	PROPN
ajlm-609	89	17	peak	peak	PROPN
ajlm-609	89	18	b	b	PROPN
ajlm-609	89	19	(	(	PUNCT
ajlm-609	89	20	65.3	65.3	NUM
ajlm-609	89	21	°	°	NOUN
ajlm-609	89	22	c	c	NOUN
ajlm-609	89	23	)	)	PUNCT
ajlm-609	89	24	.	.	PUNCT
ajlm-609	90	1	figure	figure	VERB
ajlm-609	90	2	6	6	NUM
ajlm-609	90	3	:	:	PUNCT
ajlm-609	90	4	melting	melt	VERB
ajlm-609	90	5	peaks	peak	NOUN
ajlm-609	90	6	for	for	ADP
ajlm-609	90	7	the	the	DET
ajlm-609	90	8	detection	detection	NOUN
ajlm-609	90	9	of	of	ADP
ajlm-609	90	10	stx1d	stx1d	PROPN
ajlm-609	90	11	using	use	VERB
ajlm-609	90	12	the	the	DET
ajlm-609	90	13	duplex	duplex	NOUN
ajlm-609	90	14	real	real	ADJ
ajlm-609	90	15	-	-	PUNCT
ajlm-609	90	16	time	time	NOUN
ajlm-609	90	17	polymerase	polymerase	NOUN
ajlm-609	90	18	chain	chain	NOUN
ajlm-609	90	19	reaction	reaction	NOUN
ajlm-609	90	20	assay	assay	VERB
ajlm-609	90	21	.	.	PUNCT
ajlm-609	91	1	figure	figure	VERB
ajlm-609	91	2	7	7	NUM
ajlm-609	91	3	:	:	PUNCT
ajlm-609	91	4	amplification	amplification	NOUN
ajlm-609	91	5	curves	curve	NOUN
ajlm-609	91	6	and	and	CCONJ
ajlm-609	91	7	melting	melting	NOUN
ajlm-609	91	8	peaks	peak	NOUN
ajlm-609	91	9	obtained	obtain	VERB
ajlm-609	91	10	on	on	ADP
ajlm-609	91	11	screening	screen	VERB
ajlm-609	91	12	tryptic	tryptic	ADJ
ajlm-609	91	13	soy	soy	NOUN
ajlm-609	91	14	broth	broth	NOUN
ajlm-609	91	15	using	use	VERB
ajlm-609	91	16	the	the	DET
ajlm-609	91	17	in	in	ADP
ajlm-609	91	18	-	-	PUNCT
ajlm-609	91	19	house	house	NOUN
ajlm-609	91	20	optimised	optimise	VERB
ajlm-609	91	21	real	real	ADJ
ajlm-609	91	22	-	-	PUNCT
ajlm-609	91	23	time	time	NOUN
ajlm-609	91	24	polymerase	polymerase	NOUN
ajlm-609	91	25	chain	chain	NOUN
ajlm-609	91	26	reaction	reaction	NOUN
ajlm-609	91	27	assay	assay	VERB
ajlm-609	91	28	.	.	PUNCT
ajlm-609	92	1	(	(	PUNCT
ajlm-609	92	2	a	a	X
ajlm-609	92	3	)	)	PUNCT
ajlm-609	92	4	amplification	amplification	NOUN
ajlm-609	92	5	curves	curve	NOUN
ajlm-609	92	6	.	.	PUNCT
ajlm-609	93	1	(	(	PUNCT
ajlm-609	93	2	b	b	X
ajlm-609	93	3	)	)	PUNCT
ajlm-609	93	4	melting	melting	NOUN
ajlm-609	93	5	peaks	peak	NOUN
ajlm-609	93	6	for	for	ADP
ajlm-609	93	7	the	the	DET
ajlm-609	93	8	duplex	duplex	NOUN
ajlm-609	93	9	real	real	ADJ
ajlm-609	93	10	-	-	PUNCT
ajlm-609	93	11	time	time	NOUN
ajlm-609	93	12	polymerase	polymerase	NOUN
ajlm-609	93	13	chain	chain	NOUN
ajlm-609	93	14	reaction	reaction	NOUN
ajlm-609	93	15	assay	assay	VERB
ajlm-609	93	16	.	.	PUNCT
ajlm-609	94	1	clinical	clinical	ADJ
ajlm-609	94	2	specimens	specimen	NOUN
ajlm-609	94	3	of	of	ADP
ajlm-609	94	4	the	the	DET
ajlm-609	94	5	226	226	NUM
ajlm-609	94	6	specimens	specimen	NOUN
ajlm-609	94	7	screened	screen	VERB
ajlm-609	94	8	,	,	PUNCT
ajlm-609	94	9	real	real	ADJ
ajlm-609	94	10	-	-	PUNCT
ajlm-609	94	11	time	time	NOUN
ajlm-609	94	12	pcr	pcr	PROPN
ajlm-609	94	13	detected	detect	VERB
ajlm-609	94	14	shiga	shiga	ADJ
ajlm-609	94	15	toxin	toxin	NOUN
ajlm-609	94	16	genes	gene	NOUN
ajlm-609	94	17	in	in	ADP
ajlm-609	94	18	14	14	NUM
ajlm-609	94	19	samples	sample	NOUN
ajlm-609	94	20	(	(	PUNCT
ajlm-609	94	21	6.2	6.2	NUM
ajlm-609	94	22	%	%	NOUN
ajlm-609	94	23	)	)	PUNCT
ajlm-609	94	24	,	,	PUNCT
ajlm-609	94	25	comprising	comprise	VERB
ajlm-609	94	26	8	8	NUM
ajlm-609	94	27	stx1	stx1	NOUN
ajlm-609	94	28	,	,	PUNCT
ajlm-609	94	29	5	5	NUM
ajlm-609	94	30	stx2	stx2	NOUN
ajlm-609	94	31	and	and	CCONJ
ajlm-609	94	32	1	1	NUM
ajlm-609	94	33	specimen	speciman	NOUN
ajlm-609	94	34	containing	contain	VERB
ajlm-609	94	35	both	both	CCONJ
ajlm-609	94	36	stx1	stx1	NOUN
ajlm-609	94	37	and	and	CCONJ
ajlm-609	94	38	stx2	stx2	PROPN
ajlm-609	94	39	.	.	PUNCT
ajlm-609	95	1	chromagartmstec	chromagartmstec	ADJ
ajlm-609	95	2	yielded	yield	VERB
ajlm-609	95	3	mauve	mauve	NOUN
ajlm-609	95	4	colonies	colony	NOUN
ajlm-609	95	5	from	from	ADP
ajlm-609	95	6	23.45	23.45	NUM
ajlm-609	95	7	%	%	NOUN
ajlm-609	95	8	(	(	PUNCT
ajlm-609	95	9	53/226	53/226	NUM
ajlm-609	95	10	)	)	PUNCT
ajlm-609	95	11	of	of	ADP
ajlm-609	95	12	the	the	DET
ajlm-609	95	13	stool	stool	NOUN
ajlm-609	95	14	broth	broth	NOUN
ajlm-609	95	15	cultures	culture	NOUN
ajlm-609	95	16	(	(	PUNCT
ajlm-609	95	17	figure	figure	NOUN
ajlm-609	95	18	8)	8)	NUM
ajlm-609	95	19	.	.	PUNCT
ajlm-609	95	20	figure	figure	NOUN
ajlm-609	95	21	8	8	NUM
ajlm-609	95	22	:	:	PUNCT
ajlm-609	95	23	summary	summary	NOUN
ajlm-609	95	24	of	of	ADP
ajlm-609	95	25	isolate	isolate	ADJ
ajlm-609	95	26	characterisation	characterisation	NOUN
ajlm-609	95	27	results	result	NOUN
ajlm-609	95	28	.	.	PUNCT
ajlm-609	96	1	performance	performance	NOUN
ajlm-609	96	2	of	of	ADP
ajlm-609	96	3	chromagartmstec	chromagartmstec	NOUN
ajlm-609	96	4	of	of	ADP
ajlm-609	96	5	the	the	DET
ajlm-609	96	6	53	53	NUM
ajlm-609	96	7	mauve	mauve	NOUN
ajlm-609	96	8	isolates	isolate	NOUN
ajlm-609	96	9	,	,	PUNCT
ajlm-609	96	10	48	48	NUM
ajlm-609	96	11	were	be	AUX
ajlm-609	96	12	negative	negative	ADJ
ajlm-609	96	13	for	for	ADP
ajlm-609	96	14	stx	stx	NOUN
ajlm-609	96	15	genes	gene	NOUN
ajlm-609	96	16	using	use	VERB
ajlm-609	96	17	the	the	DET
ajlm-609	96	18	validated	validate	VERB
ajlm-609	96	19	real	real	ADJ
ajlm-609	96	20	-	-	PUNCT
ajlm-609	96	21	time	time	NOUN
ajlm-609	96	22	pcr	pcr	NOUN
ajlm-609	96	23	assay	assay	NOUN
ajlm-609	96	24	.	.	PUNCT
ajlm-609	97	1	of	of	ADP
ajlm-609	97	2	the	the	DET
ajlm-609	97	3	14	14	NUM
ajlm-609	97	4	broths	broth	NOUN
ajlm-609	97	5	that	that	PRON
ajlm-609	97	6	were	be	AUX
ajlm-609	97	7	positive	positive	ADJ
ajlm-609	97	8	on	on	ADP
ajlm-609	97	9	pcr	pcr	PROPN
ajlm-609	97	10	,	,	PUNCT
ajlm-609	97	11	nine	nine	NUM
ajlm-609	97	12	did	do	AUX
ajlm-609	97	13	not	not	PART
ajlm-609	97	14	yield	yield	VERB
ajlm-609	97	15	any	any	DET
ajlm-609	97	16	mauve	mauve	NOUN
ajlm-609	97	17	colonies	colony	NOUN
ajlm-609	97	18	on	on	ADP
ajlm-609	97	19	chromagartmstec	chromagartmstec	ADJ
ajlm-609	97	20	culture	culture	NOUN
ajlm-609	97	21	.	.	PUNCT
ajlm-609	98	1	isolate	isolate	VERB
ajlm-609	98	2	characteristics	characteristic	NOUN
ajlm-609	98	3	forty	forty	NUM
ajlm-609	98	4	-	-	PUNCT
ajlm-609	98	5	four	four	NUM
ajlm-609	98	6	(	(	PUNCT
ajlm-609	98	7	83	83	NUM
ajlm-609	98	8	%	%	NOUN
ajlm-609	98	9	)	)	PUNCT
ajlm-609	98	10	of	of	ADP
ajlm-609	98	11	the	the	DET
ajlm-609	98	12	53	53	NUM
ajlm-609	98	13	mauve	mauve	NOUN
ajlm-609	98	14	colonies	colony	NOUN
ajlm-609	98	15	fermented	ferment	VERB
ajlm-609	98	16	lactose	lactose	NOUN
ajlm-609	98	17	on	on	ADP
ajlm-609	98	18	macconkey	macconkey	NOUN
ajlm-609	98	19	agar	agar	NOUN
ajlm-609	98	20	with	with	ADP
ajlm-609	98	21	crystal	crystal	PROPN
ajlm-609	98	22	violet	violet	NOUN
ajlm-609	98	23	.	.	PUNCT
ajlm-609	99	1	eleven	eleven	NUM
ajlm-609	99	2	(	(	PUNCT
ajlm-609	99	3	25	25	NUM
ajlm-609	99	4	%	%	NOUN
ajlm-609	99	5	)	)	PUNCT
ajlm-609	99	6	of	of	ADP
ajlm-609	99	7	the	the	DET
ajlm-609	99	8	44	44	NUM
ajlm-609	99	9	lactose	lactose	NOUN
ajlm-609	99	10	fermenters	fermenter	NOUN
ajlm-609	99	11	were	be	AUX
ajlm-609	99	12	non	non	ADJ
ajlm-609	99	13	-	-	ADJ
ajlm-609	99	14	sorbitol	sorbitol	ADJ
ajlm-609	99	15	-	-	PUNCT
ajlm-609	99	16	fermenting	fermenting	NOUN
ajlm-609	99	17	.	.	PUNCT
ajlm-609	100	1	real	real	ADJ
ajlm-609	100	2	-	-	PUNCT
ajlm-609	100	3	time	time	NOUN
ajlm-609	100	4	pcr	pcr	NOUN
ajlm-609	100	5	on	on	ADP
ajlm-609	100	6	the	the	DET
ajlm-609	100	7	44	44	NUM
ajlm-609	100	8	e.	e.	PROPN
ajlm-609	100	9	coli	coli	PROPN
ajlm-609	100	10	confirmed	confirm	VERB
ajlm-609	100	11	the	the	DET
ajlm-609	100	12	presence	presence	NOUN
ajlm-609	100	13	of	of	ADP
ajlm-609	100	14	stx	stx	NOUN
ajlm-609	100	15	genes	gene	NOUN
ajlm-609	100	16	in	in	ADP
ajlm-609	100	17	five	five	NUM
ajlm-609	100	18	(	(	PUNCT
ajlm-609	100	19	11	11	NUM
ajlm-609	100	20	%	%	NOUN
ajlm-609	100	21	)	)	PUNCT
ajlm-609	100	22	,	,	PUNCT
ajlm-609	100	23	whereas	whereas	SCONJ
ajlm-609	100	24	39	39	NUM
ajlm-609	100	25	were	be	AUX
ajlm-609	100	26	negative	negative	ADJ
ajlm-609	100	27	for	for	ADP
ajlm-609	100	28	the	the	DET
ajlm-609	100	29	stx	stx	NOUN
ajlm-609	100	30	gene	gene	NOUN
ajlm-609	100	31	.	.	PUNCT
ajlm-609	101	1	real	real	ADJ
ajlm-609	101	2	-	-	PUNCT
ajlm-609	101	3	time	time	NOUN
ajlm-609	101	4	pcr	pcr	NOUN
ajlm-609	101	5	was	be	AUX
ajlm-609	101	6	not	not	PART
ajlm-609	101	7	done	do	VERB
ajlm-609	101	8	on	on	ADP
ajlm-609	101	9	the	the	DET
ajlm-609	101	10	nine	nine	NUM
ajlm-609	101	11	non	non	ADJ
ajlm-609	101	12	-	-	ADJ
ajlm-609	101	13	lactose	lactose	ADJ
ajlm-609	101	14	fermenting	fermenting	NOUN
ajlm-609	101	15	isolates	isolate	NOUN
ajlm-609	101	16	as	as	SCONJ
ajlm-609	101	17	these	these	PRON
ajlm-609	101	18	were	be	AUX
ajlm-609	101	19	found	find	VERB
ajlm-609	101	20	not	not	PART
ajlm-609	101	21	to	to	PART
ajlm-609	101	22	be	be	AUX
ajlm-609	101	23	e.	e.	PROPN
ajlm-609	101	24	coli	coli	NOUN
ajlm-609	101	25	on	on	ADP
ajlm-609	101	26	biochemical	biochemical	ADJ
ajlm-609	101	27	testing	testing	NOUN
ajlm-609	101	28	.	.	PUNCT
ajlm-609	102	1	four	four	NUM
ajlm-609	102	2	of	of	ADP
ajlm-609	102	3	the	the	DET
ajlm-609	102	4	five	five	NUM
ajlm-609	102	5	stx	stx	NOUN
ajlm-609	102	6	positive	positive	ADJ
ajlm-609	102	7	e.	e.	PROPN
ajlm-609	102	8	coli	coli	NOUN
ajlm-609	102	9	colonies	colony	NOUN
ajlm-609	102	10	were	be	AUX
ajlm-609	102	11	also	also	ADV
ajlm-609	102	12	positive	positive	ADJ
ajlm-609	102	13	in	in	ADP
ajlm-609	102	14	the	the	DET
ajlm-609	102	15	real	real	ADJ
ajlm-609	102	16	-	-	PUNCT
ajlm-609	102	17	time	time	NOUN
ajlm-609	102	18	pcr	pcr	ADJ
ajlm-609	102	19	broth	broth	NOUN
ajlm-609	102	20	assay	assay	VERB
ajlm-609	102	21	.	.	PUNCT
ajlm-609	103	1	of	of	ADP
ajlm-609	103	2	the	the	DET
ajlm-609	103	3	39	39	NUM
ajlm-609	103	4	stx	stx	NOUN
ajlm-609	103	5	-	-	PUNCT
ajlm-609	103	6	negative	negative	ADJ
ajlm-609	103	7	e.	e.	PROPN
ajlm-609	103	8	coli	coli	PROPN
ajlm-609	103	9	,	,	PUNCT
ajlm-609	103	10	only	only	ADV
ajlm-609	103	11	four	four	NUM
ajlm-609	103	12	(	(	PUNCT
ajlm-609	103	13	12.5	12.5	NUM
ajlm-609	103	14	%	%	NOUN
ajlm-609	103	15	)	)	PUNCT
ajlm-609	103	16	carried	carry	VERB
ajlm-609	103	17	eae	eae	PROPN
ajlm-609	103	18	genes	gene	NOUN
ajlm-609	103	19	,	,	PUNCT
ajlm-609	103	20	whereas	whereas	SCONJ
ajlm-609	103	21	four	four	NUM
ajlm-609	103	22	possessed	possess	VERB
ajlm-609	103	23	aat	aat	NOUN
ajlm-609	103	24	genes	gene	NOUN
ajlm-609	103	25	.	.	PUNCT
ajlm-609	104	1	of	of	ADP
ajlm-609	104	2	the	the	DET
ajlm-609	104	3	four	four	NUM
ajlm-609	104	4	eae	eae	PROPN
ajlm-609	104	5	positive	positive	ADJ
ajlm-609	104	6	isolates	isolate	NOUN
ajlm-609	104	7	,	,	PUNCT
ajlm-609	104	8	two	two	NUM
ajlm-609	104	9	also	also	ADV
ajlm-609	104	10	had	have	VERB
ajlm-609	104	11	the	the	DET
ajlm-609	104	12	bfp	bfp	NOUN
ajlm-609	104	13	genes	gene	NOUN
ajlm-609	104	14	and	and	CCONJ
ajlm-609	104	15	were	be	AUX
ajlm-609	104	16	typical	typical	ADJ
ajlm-609	104	17	epec	epec	NOUN
ajlm-609	104	18	.	.	PUNCT
ajlm-609	105	1	the	the	DET
ajlm-609	105	2	other	other	ADJ
ajlm-609	105	3	two	two	NUM
ajlm-609	105	4	eae	eae	PROPN
ajlm-609	105	5	positive	positive	ADJ
ajlm-609	105	6	isolates	isolate	NOUN
ajlm-609	105	7	did	do	AUX
ajlm-609	105	8	not	not	PART
ajlm-609	105	9	possess	possess	VERB
ajlm-609	105	10	the	the	DET
ajlm-609	105	11	bfp	bfp	NOUN
ajlm-609	105	12	genes	gene	NOUN
ajlm-609	105	13	and	and	CCONJ
ajlm-609	105	14	were	be	AUX
ajlm-609	105	15	classified	classify	VERB
ajlm-609	105	16	as	as	ADP
ajlm-609	105	17	atypical	atypical	ADJ
ajlm-609	105	18	epec	epec	NOUN
ajlm-609	105	19	.	.	PUNCT
ajlm-609	106	1	the	the	DET
ajlm-609	106	2	four	four	NUM
ajlm-609	106	3	enteroaggregative	enteroaggregative	ADJ
ajlm-609	106	4	stx	stx	ADJ
ajlm-609	106	5	-	-	PUNCT
ajlm-609	106	6	negative	negative	ADJ
ajlm-609	106	7	isolates	isolate	NOUN
ajlm-609	106	8	all	all	PRON
ajlm-609	106	9	belonged	belong	VERB
ajlm-609	106	10	to	to	ADP
ajlm-609	106	11	e.	e.	PROPN
ajlm-609	106	12	coli	coli	PROPN
ajlm-609	106	13	serotype	serotype	NOUN
ajlm-609	106	14	o104	o104	PROPN
ajlm-609	106	15	.	.	PUNCT
ajlm-609	107	1	all	all	DET
ajlm-609	107	2	the	the	DET
ajlm-609	107	3	typical	typical	ADJ
ajlm-609	107	4	epec	epec	NOUN
ajlm-609	107	5	belonged	belong	VERB
ajlm-609	107	6	to	to	ADP
ajlm-609	107	7	serotype	serotype	ADJ
ajlm-609	107	8	o55	o55	NOUN
ajlm-609	107	9	,	,	PUNCT
ajlm-609	107	10	whereas	whereas	SCONJ
ajlm-609	107	11	one	one	NUM
ajlm-609	107	12	of	of	ADP
ajlm-609	107	13	the	the	DET
ajlm-609	107	14	two	two	NUM
ajlm-609	107	15	atypical	atypical	ADJ
ajlm-609	107	16	epec	epec	NOUN
ajlm-609	107	17	belonged	belong	VERB
ajlm-609	107	18	to	to	PART
ajlm-609	107	19	serotype	serotype	VERB
ajlm-609	107	20	o101	o101	PROPN
ajlm-609	107	21	.	.	PUNCT
ajlm-609	108	1	the	the	DET
ajlm-609	108	2	atypical	atypical	ADJ
ajlm-609	108	3	epec	epec	NOUN
ajlm-609	108	4	serotype	serotype	NOUN
ajlm-609	108	5	o101	o101	PROPN
ajlm-609	108	6	was	be	AUX
ajlm-609	108	7	from	from	ADP
ajlm-609	108	8	stx2	stx2	ADJ
ajlm-609	108	9	positive	positive	ADJ
ajlm-609	108	10	broth	broth	NOUN
ajlm-609	108	11	.	.	PUNCT
ajlm-609	109	1	the	the	DET
ajlm-609	109	2	other	other	ADJ
ajlm-609	109	3	atypical	atypical	ADJ
ajlm-609	109	4	epec	epec	NOUN
ajlm-609	109	5	isolate	isolate	NOUN
ajlm-609	109	6	was	be	AUX
ajlm-609	109	7	untypeable	untypeable	ADJ
ajlm-609	109	8	.	.	PUNCT
ajlm-609	110	1	no	no	DET
ajlm-609	110	2	diffusely	diffusely	ADV
ajlm-609	110	3	adherent	adherent	ADJ
ajlm-609	110	4	e.	e.	PROPN
ajlm-609	110	5	coli	coli	PROPN
ajlm-609	110	6	,	,	PUNCT
ajlm-609	110	7	enteroinvasive	enteroinvasive	ADJ
ajlm-609	110	8	e.	e.	PROPN
ajlm-609	110	9	coli	coli	PROPN
ajlm-609	110	10	or	or	CCONJ
ajlm-609	110	11	enterotoxigenic	enterotoxigenic	ADJ
ajlm-609	110	12	e.	e.	PROPN
ajlm-609	110	13	coli	coli	PROPN
ajlm-609	110	14	were	be	AUX
ajlm-609	110	15	detected	detect	VERB
ajlm-609	110	16	.	.	PUNCT
ajlm-609	111	1	none	none	NOUN
ajlm-609	111	2	of	of	ADP
ajlm-609	111	3	the	the	DET
ajlm-609	111	4	53	53	NUM
ajlm-609	111	5	e.	e.	PROPN
ajlm-609	111	6	coli	coli	NOUN
ajlm-609	111	7	isolates	isolate	NOUN
ajlm-609	111	8	that	that	PRON
ajlm-609	111	9	were	be	AUX
ajlm-609	111	10	screened	screen	VERB
ajlm-609	111	11	by	by	ADP
ajlm-609	111	12	immunochromatography	immunochromatography	NOUN
ajlm-609	111	13	was	be	AUX
ajlm-609	111	14	positive	positive	ADJ
ajlm-609	111	15	for	for	ADP
ajlm-609	111	16	shiga	shiga	ADJ
ajlm-609	111	17	toxins	toxin	NOUN
ajlm-609	111	18	.	.	PUNCT
ajlm-609	112	1	for	for	ADP
ajlm-609	112	2	the	the	DET
ajlm-609	112	3	chromagartmstec	chromagartmstec	ADJ
ajlm-609	112	4	,	,	PUNCT
ajlm-609	112	5	sensitivity	sensitivity	NOUN
ajlm-609	112	6	was	be	AUX
ajlm-609	112	7	33.3	33.3	NUM
ajlm-609	112	8	%	%	NOUN
ajlm-609	112	9	,	,	PUNCT
ajlm-609	112	10	specificity	specificity	NOUN
ajlm-609	112	11	was	be	AUX
ajlm-609	112	12	77.4	77.4	NUM
ajlm-609	112	13	%	%	NOUN
ajlm-609	112	14	,	,	PUNCT
ajlm-609	112	15	negative	negative	ADJ
ajlm-609	112	16	predictive	predictive	ADJ
ajlm-609	112	17	value	value	NOUN
ajlm-609	112	18	was	be	AUX
ajlm-609	112	19	95	95	NUM
ajlm-609	112	20	%	%	NOUN
ajlm-609	112	21	and	and	CCONJ
ajlm-609	112	22	positive	positive	ADJ
ajlm-609	112	23	predictive	predictive	ADJ
ajlm-609	112	24	value	value	NOUN
ajlm-609	112	25	was	be	AUX
ajlm-609	112	26	9.4	9.4	NUM
ajlm-609	112	27	%	%	NOUN
ajlm-609	112	28	(	(	PUNCT
ajlm-609	112	29	table	table	NOUN
ajlm-609	112	30	3	3	NUM
ajlm-609	112	31	)	)	PUNCT
ajlm-609	112	32	.	.	PUNCT
ajlm-609	113	1	table	table	NOUN
ajlm-609	113	2	3	3	NUM
ajlm-609	113	3	:	:	PUNCT
ajlm-609	113	4	 	 	SPACE
ajlm-609	113	5	performance	performance	NOUN
ajlm-609	113	6	of	of	ADP
ajlm-609	113	7	chromagartmstec	chromagartmstec	NOUN
ajlm-609	113	8	compared	compare	VERB
ajlm-609	113	9	with	with	ADP
ajlm-609	113	10	real	real	ADJ
ajlm-609	113	11	-	-	PUNCT
ajlm-609	113	12	time	time	NOUN
ajlm-609	113	13	polymerase	polymerase	NOUN
ajlm-609	113	14	chain	chain	NOUN
ajlm-609	113	15	reaction	reaction	NOUN
ajlm-609	113	16	assay	assay	VERB
ajlm-609	113	17	.	.	PUNCT
ajlm-609	114	1	discussion	discussion	NOUN
ajlm-609	114	2	we	we	PRON
ajlm-609	114	3	validated	validate	VERB
ajlm-609	114	4	the	the	DET
ajlm-609	114	5	use	use	NOUN
ajlm-609	114	6	of	of	ADP
ajlm-609	114	7	a	a	DET
ajlm-609	114	8	previously	previously	ADV
ajlm-609	114	9	described	describe	VERB
ajlm-609	114	10	duplex	duplex	NOUN
ajlm-609	114	11	real	real	ADJ
ajlm-609	114	12	-	-	PUNCT
ajlm-609	114	13	time	time	NOUN
ajlm-609	114	14	pcr	pcr	PROPN
ajlm-609	114	15	assay	assay	VERB
ajlm-609	114	16	with	with	ADP
ajlm-609	114	17	modification	modification	NOUN
ajlm-609	114	18	able	able	ADJ
ajlm-609	114	19	to	to	PART
ajlm-609	114	20	detect	detect	VERB
ajlm-609	114	21	and	and	CCONJ
ajlm-609	114	22	differentiate	differentiate	VERB
ajlm-609	114	23	stx1	stx1	NOUN
ajlm-609	114	24	(	(	PUNCT
ajlm-609	114	25	melting	melt	VERB
ajlm-609	114	26	temperature	temperature	NOUN
ajlm-609	114	27	=	=	NOUN
ajlm-609	114	28	58.2	58.2	NUM
ajlm-609	114	29	°	°	NUM
ajlm-609	114	30	c	c	NOUN
ajlm-609	114	31	)	)	PUNCT
ajlm-609	114	32	and	and	CCONJ
ajlm-609	114	33	stx2	stx2	PROPN
ajlm-609	114	34	(	(	PUNCT
ajlm-609	114	35	melting	melt	VERB
ajlm-609	114	36	temperature	temperature	NOUN
ajlm-609	114	37	=	=	PUNCT
ajlm-609	114	38	65.3	65.3	NUM
ajlm-609	114	39	°	°	NUM
ajlm-609	114	40	c	c	NOUN
ajlm-609	114	41	)	)	PUNCT
ajlm-609	114	42	from	from	ADP
ajlm-609	114	43	overnight	overnight	ADJ
ajlm-609	114	44	broth	broth	ADJ
ajlm-609	114	45	enrichment	enrichment	NOUN
ajlm-609	114	46	with	with	ADP
ajlm-609	114	47	a	a	DET
ajlm-609	114	48	lod	lod	NOUN
ajlm-609	114	49	of	of	ADP
ajlm-609	114	50	5.3	5.3	NUM
ajlm-609	114	51	target	target	NOUN
ajlm-609	114	52	copies	copy	NOUN
ajlm-609	114	53	/	/	SYM
ajlm-609	114	54	μl	μl	NUM
ajlm-609	114	55	broth	broth	NOUN
ajlm-609	114	56	.	.	PUNCT
ajlm-609	115	1	this	this	DET
ajlm-609	115	2	assay	assay	NOUN
ajlm-609	115	3	was	be	AUX
ajlm-609	115	4	able	able	ADJ
ajlm-609	115	5	to	to	PART
ajlm-609	115	6	detect	detect	VERB
ajlm-609	115	7	both	both	DET
ajlm-609	115	8	stx1	stx1	NOUN
ajlm-609	115	9	and	and	CCONJ
ajlm-609	115	10	stx2	stx2	VERB
ajlm-609	115	11	in	in	ADP
ajlm-609	115	12	the	the	DET
ajlm-609	115	13	same	same	ADJ
ajlm-609	115	14	run	run	NOUN
ajlm-609	115	15	,	,	PUNCT
ajlm-609	115	16	thus	thus	ADV
ajlm-609	115	17	potentially	potentially	ADV
ajlm-609	115	18	reducing	reduce	VERB
ajlm-609	115	19	process	process	NOUN
ajlm-609	115	20	turn	turn	NOUN
ajlm-609	115	21	-	-	PUNCT
ajlm-609	115	22	around	around	ADP
ajlm-609	115	23	time	time	NOUN
ajlm-609	115	24	in	in	ADP
ajlm-609	115	25	a	a	DET
ajlm-609	115	26	busy	busy	ADJ
ajlm-609	115	27	laboratory	laboratory	NOUN
ajlm-609	115	28	setting	setting	NOUN
ajlm-609	115	29	.	.	PUNCT
ajlm-609	116	1	timely	timely	ADJ
ajlm-609	116	2	reporting	reporting	NOUN
ajlm-609	116	3	of	of	ADP
ajlm-609	116	4	stec	stec	ADJ
ajlm-609	116	5	infections	infection	NOUN
ajlm-609	116	6	is	be	AUX
ajlm-609	116	7	important	important	ADJ
ajlm-609	116	8	,	,	PUNCT
ajlm-609	116	9	because	because	SCONJ
ajlm-609	116	10	use	use	NOUN
ajlm-609	116	11	of	of	ADP
ajlm-609	116	12	certain	certain	ADJ
ajlm-609	116	13	antibiotics	antibiotic	NOUN
ajlm-609	116	14	is	be	AUX
ajlm-609	116	15	contraindicated	contraindicate	VERB
ajlm-609	116	16	in	in	ADP
ajlm-609	116	17	stec	stec	ADJ
ajlm-609	116	18	infections	infection	NOUN
ajlm-609	116	19	.	.	PUNCT
ajlm-609	117	1	compared	compare	VERB
ajlm-609	117	2	to	to	ADP
ajlm-609	117	3	the	the	DET
ajlm-609	117	4	validated	validate	VERB
ajlm-609	117	5	duplex	duplex	NOUN
ajlm-609	117	6	real	real	ADJ
ajlm-609	117	7	-	-	PUNCT
ajlm-609	117	8	time	time	NOUN
ajlm-609	117	9	pcr	pcr	NOUN
ajlm-609	117	10	,	,	PUNCT
ajlm-609	117	11	chromagartmstec	chromagartmstec	PROPN
ajlm-609	117	12	showed	show	VERB
ajlm-609	117	13	a	a	DET
ajlm-609	117	14	sensitivity	sensitivity	NOUN
ajlm-609	117	15	of	of	ADP
ajlm-609	117	16	33	33	NUM
ajlm-609	117	17	%	%	NOUN
ajlm-609	117	18	,	,	PUNCT
ajlm-609	117	19	specificity	specificity	NOUN
ajlm-609	117	20	of	of	ADP
ajlm-609	117	21	77.4	77.4	NUM
ajlm-609	117	22	%	%	NOUN
ajlm-609	117	23	,	,	PUNCT
ajlm-609	117	24	negative	negative	ADJ
ajlm-609	117	25	predictive	predictive	ADJ
ajlm-609	117	26	value	value	NOUN
ajlm-609	117	27	of	of	ADP
ajlm-609	117	28	9.4	9.4	NUM
ajlm-609	117	29	%	%	NOUN
ajlm-609	117	30	and	and	CCONJ
ajlm-609	117	31	positive	positive	ADJ
ajlm-609	117	32	predictive	predictive	ADJ
ajlm-609	117	33	value	value	NOUN
ajlm-609	117	34	of	of	ADP
ajlm-609	117	35	95	95	NUM
ajlm-609	117	36	%	%	NOUN
ajlm-609	117	37	for	for	ADP
ajlm-609	117	38	detection	detection	NOUN
ajlm-609	117	39	of	of	ADP
ajlm-609	117	40	stec	stec	ADJ
ajlm-609	117	41	in	in	ADP
ajlm-609	117	42	stool	stool	NOUN
ajlm-609	117	43	following	follow	VERB
ajlm-609	117	44	tsb	tsb	NOUN
ajlm-609	117	45	enrichment	enrichment	NOUN
ajlm-609	117	46	.	.	PUNCT
ajlm-609	118	1	of	of	ADP
ajlm-609	118	2	the	the	DET
ajlm-609	118	3	53	53	NUM
ajlm-609	118	4	mauve	mauve	NOUN
ajlm-609	118	5	isolates	isolate	NOUN
ajlm-609	118	6	,	,	PUNCT
ajlm-609	118	7	48	48	NUM
ajlm-609	118	8	were	be	AUX
ajlm-609	118	9	negative	negative	ADJ
ajlm-609	118	10	for	for	ADP
ajlm-609	118	11	stx	stx	NOUN
ajlm-609	118	12	genes	gene	NOUN
ajlm-609	118	13	on	on	ADP
ajlm-609	118	14	use	use	NOUN
ajlm-609	118	15	of	of	ADP
ajlm-609	118	16	the	the	DET
ajlm-609	118	17	validated	validate	VERB
ajlm-609	118	18	real	real	ADJ
ajlm-609	118	19	-	-	PUNCT
ajlm-609	118	20	time	time	NOUN
ajlm-609	118	21	pcr	pcr	NOUN
ajlm-609	118	22	,	,	PUNCT
ajlm-609	118	23	whereas	whereas	SCONJ
ajlm-609	118	24	nine	nine	NUM
ajlm-609	118	25	of	of	ADP
ajlm-609	118	26	the	the	DET
ajlm-609	118	27	14	14	NUM
ajlm-609	118	28	pcr	pcr	ADJ
ajlm-609	118	29	-	-	PUNCT
ajlm-609	118	30	positive	positive	ADJ
ajlm-609	118	31	broths	broth	NOUN
ajlm-609	118	32	did	do	AUX
ajlm-609	118	33	not	not	PART
ajlm-609	118	34	yield	yield	VERB
ajlm-609	118	35	any	any	DET
ajlm-609	118	36	mauve	mauve	NOUN
ajlm-609	118	37	colonies	colony	NOUN
ajlm-609	118	38	when	when	SCONJ
ajlm-609	118	39	cultured	cultured	ADJ
ajlm-609	118	40	on	on	ADP
ajlm-609	118	41	chromagartmstec	chromagartmstec	ADJ
ajlm-609	118	42	.	.	PUNCT
ajlm-609	119	1	reasons	reason	NOUN
ajlm-609	119	2	for	for	ADP
ajlm-609	119	3	the	the	DET
ajlm-609	119	4	poor	poor	ADJ
ajlm-609	119	5	performance	performance	NOUN
ajlm-609	119	6	of	of	ADP
ajlm-609	119	7	this	this	DET
ajlm-609	119	8	medium	medium	NOUN
ajlm-609	119	9	in	in	ADP
ajlm-609	119	10	relation	relation	NOUN
ajlm-609	119	11	to	to	ADP
ajlm-609	119	12	the	the	DET
ajlm-609	119	13	in	in	ADP
ajlm-609	119	14	-	-	PUNCT
ajlm-609	119	15	house	house	NOUN
ajlm-609	119	16	developed	develop	VERB
ajlm-609	119	17	duplex	duplex	NOUN
ajlm-609	119	18	real	real	ADJ
ajlm-609	119	19	-	-	PUNCT
ajlm-609	119	20	time	time	NOUN
ajlm-609	119	21	pcr	pcr	NOUN
ajlm-609	119	22	include	include	VERB
ajlm-609	119	23	the	the	DET
ajlm-609	119	24	following	follow	VERB
ajlm-609	119	25	:	:	PUNCT
ajlm-609	119	26	(	(	PUNCT
ajlm-609	119	27	1	1	X
ajlm-609	119	28	)	)	PUNCT
ajlm-609	119	29	delays	delay	NOUN
ajlm-609	119	30	in	in	ADP
ajlm-609	119	31	reporting	reporting	NOUN
ajlm-609	119	32	of	of	ADP
ajlm-609	119	33	diarrhoea	diarrhoea	NOUN
ajlm-609	119	34	cases	case	NOUN
ajlm-609	119	35	to	to	ADP
ajlm-609	119	36	a	a	DET
ajlm-609	119	37	tertiary	tertiary	ADJ
ajlm-609	119	38	hospital	hospital	NOUN
ajlm-609	119	39	(	(	PUNCT
ajlm-609	119	40	where	where	SCONJ
ajlm-609	119	41	samples	sample	NOUN
ajlm-609	119	42	were	be	AUX
ajlm-609	119	43	collected	collect	VERB
ajlm-609	119	44	)	)	PUNCT
ajlm-609	119	45	may	may	AUX
ajlm-609	119	46	have	have	AUX
ajlm-609	119	47	led	lead	VERB
ajlm-609	119	48	to	to	ADP
ajlm-609	119	49	loss	loss	NOUN
ajlm-609	119	50	of	of	ADP
ajlm-609	119	51	stx	stx	NOUN
ajlm-609	119	52	genes	gene	NOUN
ajlm-609	119	53	;	;	PUNCT
ajlm-609	119	54	stec	stec	ADJ
ajlm-609	119	55	numbers	number	NOUN
ajlm-609	119	56	are	be	AUX
ajlm-609	119	57	sharply	sharply	ADV
ajlm-609	119	58	reduced	reduce	VERB
ajlm-609	119	59	in	in	ADP
ajlm-609	119	60	stool	stool	NOUN
ajlm-609	119	61	after	after	ADP
ajlm-609	119	62	one	one	NUM
ajlm-609	119	63	week	week	NOUN
ajlm-609	119	64	of	of	ADP
ajlm-609	119	65	illness	illness	NOUN
ajlm-609	119	66	,	,	PUNCT
ajlm-609	119	67	and	and	CCONJ
ajlm-609	119	68	the	the	DET
ajlm-609	119	69	shiga	shiga	ADJ
ajlm-609	119	70	toxin	toxin	NOUN
ajlm-609	119	71	genes	gene	NOUN
ajlm-609	119	72	might	might	AUX
ajlm-609	119	73	be	be	AUX
ajlm-609	119	74	lost	lose	VERB
ajlm-609	119	75	by	by	ADP
ajlm-609	119	76	the	the	DET
ajlm-609	119	77	bacteria.21	bacteria.21	NOUN
ajlm-609	119	78	(	(	PUNCT
ajlm-609	119	79	2	2	NUM
ajlm-609	119	80	)	)	PUNCT
ajlm-609	119	81	chromagartmstec	chromagartmstec	ADJ
ajlm-609	119	82	selects	select	NOUN
ajlm-609	119	83	for	for	ADP
ajlm-609	119	84	tellurite	tellurite	NOUN
ajlm-609	119	85	resistant	resistant	ADJ
ajlm-609	119	86	strains	strain	NOUN
ajlm-609	119	87	but	but	CCONJ
ajlm-609	119	88	misses	miss	VERB
ajlm-609	119	89	the	the	DET
ajlm-609	119	90	tellurite	tellurite	NOUN
ajlm-609	119	91	susceptible	susceptible	ADJ
ajlm-609	119	92	stec	stec	ADJ
ajlm-609	119	93	whose	whose	DET
ajlm-609	119	94	prevalence	prevalence	NOUN
ajlm-609	119	95	in	in	ADP
ajlm-609	119	96	this	this	DET
ajlm-609	119	97	setting	setting	NOUN
ajlm-609	119	98	is	be	AUX
ajlm-609	119	99	not	not	PART
ajlm-609	119	100	known	know	VERB
ajlm-609	119	101	.	.	PUNCT
ajlm-609	120	1	for	for	SCONJ
ajlm-609	120	2	a	a	DET
ajlm-609	120	3	chromogenic	chromogenic	ADJ
ajlm-609	120	4	medium	medium	NOUN
ajlm-609	120	5	to	to	PART
ajlm-609	120	6	be	be	AUX
ajlm-609	120	7	considered	consider	VERB
ajlm-609	120	8	for	for	ADP
ajlm-609	120	9	routine	routine	ADJ
ajlm-609	120	10	screening	screening	NOUN
ajlm-609	120	11	purposes	purpose	NOUN
ajlm-609	120	12	,	,	PUNCT
ajlm-609	120	13	it	it	PRON
ajlm-609	120	14	must	must	AUX
ajlm-609	120	15	have	have	VERB
ajlm-609	120	16	high	high	ADJ
ajlm-609	120	17	specificity	specificity	NOUN
ajlm-609	120	18	so	so	SCONJ
ajlm-609	120	19	as	as	SCONJ
ajlm-609	120	20	not	not	PART
ajlm-609	120	21	to	to	PART
ajlm-609	120	22	waste	waste	VERB
ajlm-609	120	23	scarce	scarce	ADJ
ajlm-609	120	24	laboratory	laboratory	NOUN
ajlm-609	120	25	resources	resource	NOUN
ajlm-609	120	26	on	on	ADP
ajlm-609	120	27	false	false	ADJ
ajlm-609	120	28	positives	positive	NOUN
ajlm-609	120	29	.	.	PUNCT
ajlm-609	121	1	the	the	DET
ajlm-609	121	2	false	false	ADJ
ajlm-609	121	3	positivity	positivity	NOUN
ajlm-609	121	4	rate	rate	NOUN
ajlm-609	121	5	in	in	ADP
ajlm-609	121	6	this	this	DET
ajlm-609	121	7	setting	setting	NOUN
ajlm-609	121	8	(	(	PUNCT
ajlm-609	121	9	48/53	48/53	NUM
ajlm-609	121	10	[	[	X
ajlm-609	121	11	90.6	90.6	NUM
ajlm-609	121	12	%	%	NOUN
ajlm-609	121	13	]	]	PUNCT
ajlm-609	121	14	)	)	PUNCT
ajlm-609	121	15	is	be	AUX
ajlm-609	121	16	higher	high	ADJ
ajlm-609	121	17	than	than	SCONJ
ajlm-609	121	18	has	have	AUX
ajlm-609	121	19	previously	previously	ADV
ajlm-609	121	20	been	be	AUX
ajlm-609	121	21	reported	report	VERB
ajlm-609	121	22	in	in	ADP
ajlm-609	121	23	europe	europe	PROPN
ajlm-609	121	24	(	(	PUNCT
ajlm-609	121	25	16.3	16.3	NUM
ajlm-609	121	26	%	%	NOUN
ajlm-609	121	27	reported	report	VERB
ajlm-609	121	28	by	by	ADP
ajlm-609	121	29	gouali	gouali	PROPN
ajlm-609	121	30	et	et	PROPN
ajlm-609	121	31	al	al	PROPN
ajlm-609	121	32	.	.	PROPN
ajlm-609	121	33	,	,	PUNCT
ajlm-609	121	34	2013	2013	NUM
ajlm-609	121	35	and	and	CCONJ
ajlm-609	121	36	18.3	18.3	NUM
ajlm-609	121	37	%	%	NOUN
ajlm-609	121	38	by	by	ADP
ajlm-609	121	39	wylie	wylie	PROPN
ajlm-609	121	40	et	et	PROPN
ajlm-609	121	41	al	al	PROPN
ajlm-609	121	42	.	.	PROPN
ajlm-609	121	43	,	,	PUNCT
ajlm-609	121	44	2013).12,22	2013).12,22	NUM
ajlm-609	121	45	similar	similar	ADJ
ajlm-609	121	46	studies	study	NOUN
ajlm-609	121	47	to	to	PART
ajlm-609	121	48	evaluate	evaluate	VERB
ajlm-609	121	49	this	this	DET
ajlm-609	121	50	medium	medium	NOUN
ajlm-609	121	51	were	be	AUX
ajlm-609	121	52	done	do	VERB
ajlm-609	121	53	in	in	ADP
ajlm-609	121	54	canada	canada	PROPN
ajlm-609	121	55	,	,	PUNCT
ajlm-609	121	56	finland	finland	PROPN
ajlm-609	121	57	,	,	PUNCT
ajlm-609	121	58	and	and	CCONJ
ajlm-609	121	59	germany	germany	PROPN
ajlm-609	121	60	,	,	PUNCT
ajlm-609	121	61	all	all	PRON
ajlm-609	121	62	of	of	ADP
ajlm-609	121	63	which	which	PRON
ajlm-609	121	64	reported	report	VERB
ajlm-609	121	65	high	high	ADJ
ajlm-609	121	66	sensitivities	sensitivity	NOUN
ajlm-609	121	67	for	for	ADP
ajlm-609	121	68	stec	stec	ADJ
ajlm-609	121	69	serotypes	serotype	NOUN
ajlm-609	121	70	o26	o26	ADJ
ajlm-609	121	71	,	,	PUNCT
ajlm-609	121	72	o111	o111	PROPN
ajlm-609	121	73	,	,	PUNCT
ajlm-609	121	74	o121	o121	NUM
ajlm-609	121	75	,	,	PUNCT
ajlm-609	121	76	o145	o145	PROPN
ajlm-609	121	77	,	,	PUNCT
ajlm-609	121	78	o118	o118	NUM
ajlm-609	121	79	,	,	PUNCT
ajlm-609	121	80	and	and	CCONJ
ajlm-609	121	81	o157.13,22,23	o157.13,22,23	PROPN
ajlm-609	121	82	the	the	DET
ajlm-609	121	83	specificity	specificity	NOUN
ajlm-609	121	84	of	of	ADP
ajlm-609	121	85	77.4	77.4	NUM
ajlm-609	121	86	%	%	NOUN
ajlm-609	121	87	noted	note	VERB
ajlm-609	121	88	in	in	ADP
ajlm-609	121	89	this	this	DET
ajlm-609	121	90	study	study	NOUN
ajlm-609	121	91	was	be	AUX
ajlm-609	121	92	low	low	ADJ
ajlm-609	121	93	compared	compare	VERB
ajlm-609	121	94	to	to	ADP
ajlm-609	121	95	values	value	NOUN
ajlm-609	121	96	(	(	PUNCT
ajlm-609	121	97	between	between	ADP
ajlm-609	121	98	95.8	95.8	NUM
ajlm-609	121	99	%	%	NOUN
ajlm-609	121	100	and	and	CCONJ
ajlm-609	121	101	98.9	98.9	NUM
ajlm-609	121	102	%	%	NOUN
ajlm-609	121	103	)	)	PUNCT
ajlm-609	121	104	reported	report	VERB
ajlm-609	121	105	in	in	ADP
ajlm-609	121	106	similar	similar	ADJ
ajlm-609	121	107	studies	study	NOUN
ajlm-609	121	108	done	do	VERB
ajlm-609	121	109	in	in	ADP
ajlm-609	121	110	europe	europe	PROPN
ajlm-609	121	111	.	.	PUNCT
ajlm-609	122	1	the	the	DET
ajlm-609	122	2	difference	difference	NOUN
ajlm-609	122	3	in	in	ADP
ajlm-609	122	4	sensitivity	sensitivity	NOUN
ajlm-609	122	5	and	and	CCONJ
ajlm-609	122	6	specificity	specificity	NOUN
ajlm-609	122	7	could	could	AUX
ajlm-609	122	8	be	be	AUX
ajlm-609	122	9	explained	explain	VERB
ajlm-609	122	10	by	by	ADP
ajlm-609	122	11	the	the	DET
ajlm-609	122	12	differences	difference	NOUN
ajlm-609	122	13	in	in	ADP
ajlm-609	122	14	the	the	DET
ajlm-609	122	15	patient	patient	ADJ
ajlm-609	122	16	characteristics	characteristic	NOUN
ajlm-609	122	17	(	(	PUNCT
ajlm-609	122	18	whether	whether	SCONJ
ajlm-609	122	19	they	they	PRON
ajlm-609	122	20	present	present	VERB
ajlm-609	122	21	with	with	ADP
ajlm-609	122	22	haemolytic	haemolytic	ADJ
ajlm-609	122	23	uremic	uremic	ADJ
ajlm-609	122	24	syndrome	syndrome	NOUN
ajlm-609	122	25	and	and	CCONJ
ajlm-609	122	26	or	or	CCONJ
ajlm-609	122	27	bloody	bloody	ADJ
ajlm-609	122	28	diarrhoea	diarrhoea	NOUN
ajlm-609	122	29	or	or	CCONJ
ajlm-609	122	30	not	not	PART
ajlm-609	122	31	)	)	PUNCT
ajlm-609	122	32	.	.	PUNCT
ajlm-609	123	1	unlike	unlike	ADP
ajlm-609	123	2	the	the	DET
ajlm-609	123	3	studies	study	NOUN
ajlm-609	123	4	in	in	ADP
ajlm-609	123	5	europe	europe	PROPN
ajlm-609	123	6	,	,	PUNCT
ajlm-609	123	7	this	this	DET
ajlm-609	123	8	study	study	NOUN
ajlm-609	123	9	did	do	AUX
ajlm-609	123	10	not	not	PART
ajlm-609	123	11	focus	focus	VERB
ajlm-609	123	12	on	on	ADP
ajlm-609	123	13	only	only	ADJ
ajlm-609	123	14	patients	patient	NOUN
ajlm-609	123	15	with	with	ADP
ajlm-609	123	16	haemolytic	haemolytic	ADJ
ajlm-609	123	17	uremic	uremic	ADJ
ajlm-609	123	18	syndrome	syndrome	NOUN
ajlm-609	123	19	or	or	CCONJ
ajlm-609	123	20	bloody	bloody	ADJ
ajlm-609	123	21	diarrhoea	diarrhoea	NOUN
ajlm-609	123	22	.	.	PUNCT
ajlm-609	124	1	additionally	additionally	ADV
ajlm-609	124	2	,	,	PUNCT
ajlm-609	124	3	the	the	DET
ajlm-609	124	4	distribution	distribution	NOUN
ajlm-609	124	5	of	of	ADP
ajlm-609	124	6	tellurite	tellurite	NOUN
ajlm-609	124	7	resistant	resistant	ADJ
ajlm-609	124	8	stec	stec	ADJ
ajlm-609	124	9	(	(	PUNCT
ajlm-609	124	10	which	which	PRON
ajlm-609	124	11	is	be	AUX
ajlm-609	124	12	targeted	target	VERB
ajlm-609	124	13	by	by	ADP
ajlm-609	124	14	chromagartmstec	chromagartmstec	ADJ
ajlm-609	124	15	)	)	PUNCT
ajlm-609	124	16	in	in	ADP
ajlm-609	124	17	this	this	DET
ajlm-609	124	18	setting	setting	NOUN
ajlm-609	124	19	is	be	AUX
ajlm-609	124	20	not	not	PART
ajlm-609	124	21	known	know	VERB
ajlm-609	124	22	.	.	PUNCT
ajlm-609	125	1	the	the	DET
ajlm-609	125	2	prevalence	prevalence	NOUN
ajlm-609	125	3	of	of	ADP
ajlm-609	125	4	stx	stx	NOUN
ajlm-609	125	5	genes	gene	NOUN
ajlm-609	125	6	in	in	ADP
ajlm-609	125	7	stool	stool	NOUN
ajlm-609	125	8	samples	sample	NOUN
ajlm-609	125	9	(	(	PUNCT
ajlm-609	125	10	6.2	6.2	NUM
ajlm-609	125	11	%	%	NOUN
ajlm-609	125	12	)	)	PUNCT
ajlm-609	125	13	was	be	AUX
ajlm-609	125	14	lower	low	ADJ
ajlm-609	125	15	than	than	ADP
ajlm-609	125	16	the	the	DET
ajlm-609	125	17	9	9	NUM
ajlm-609	125	18	%	%	NOUN
ajlm-609	125	19	previously	previously	ADV
ajlm-609	125	20	reported	report	VERB
ajlm-609	125	21	by	by	ADP
ajlm-609	125	22	kullin	kullin	PROPN
ajlm-609	125	23	et	et	PROPN
ajlm-609	125	24	al	al	PROPN
ajlm-609	125	25	.	.	PROPN
ajlm-609	125	26	,	,	PUNCT
ajlm-609	125	27	2015.24	2015.24	NUM
ajlm-609	125	28	among	among	ADP
ajlm-609	125	29	the	the	DET
ajlm-609	125	30	53	53	NUM
ajlm-609	125	31	isolates	isolate	NOUN
ajlm-609	125	32	that	that	PRON
ajlm-609	125	33	formed	form	VERB
ajlm-609	125	34	mauve	mauve	NOUN
ajlm-609	125	35	colonies	colony	NOUN
ajlm-609	125	36	on	on	ADP
ajlm-609	125	37	chromagartmstec	chromagartmstec	ADJ
ajlm-609	125	38	,	,	PUNCT
ajlm-609	125	39	five	five	NUM
ajlm-609	125	40	were	be	AUX
ajlm-609	125	41	stec	stec	ADJ
ajlm-609	125	42	(	(	PUNCT
ajlm-609	125	43	two	two	NUM
ajlm-609	125	44	serotype	serotype	ADJ
ajlm-609	125	45	o26	o26	NOUN
ajlm-609	125	46	and	and	CCONJ
ajlm-609	125	47	others	other	NOUN
ajlm-609	125	48	non	non	ADJ
ajlm-609	125	49	-	-	ADJ
ajlm-609	125	50	typeable	typeable	ADJ
ajlm-609	125	51	)	)	PUNCT
ajlm-609	125	52	,	,	PUNCT
ajlm-609	125	53	four	four	NUM
ajlm-609	125	54	were	be	AUX
ajlm-609	125	55	enteroaggregative	enteroaggregative	ADJ
ajlm-609	125	56	e.	e.	PROPN
ajlm-609	125	57	coli	coli	PROPN
ajlm-609	125	58	(	(	PUNCT
ajlm-609	125	59	serotype	serotype	NOUN
ajlm-609	125	60	o104	o104	PROPN
ajlm-609	125	61	)	)	PUNCT
ajlm-609	125	62	,	,	PUNCT
ajlm-609	125	63	two	two	NUM
ajlm-609	125	64	were	be	AUX
ajlm-609	125	65	atypical	atypical	ADJ
ajlm-609	125	66	epec	epec	NOUN
ajlm-609	125	67	(	(	PUNCT
ajlm-609	125	68	serotype	serotype	NOUN
ajlm-609	125	69	o101	o101	PROPN
ajlm-609	125	70	and	and	CCONJ
ajlm-609	125	71	non	non	ADJ
ajlm-609	125	72	-	-	ADJ
ajlm-609	125	73	typeable	typeable	ADJ
ajlm-609	125	74	)	)	PUNCT
ajlm-609	125	75	and	and	CCONJ
ajlm-609	125	76	two	two	NUM
ajlm-609	125	77	were	be	AUX
ajlm-609	125	78	typical	typical	ADJ
ajlm-609	125	79	epec	epec	NOUN
ajlm-609	125	80	(	(	PUNCT
ajlm-609	125	81	serotype	serotype	ADJ
ajlm-609	125	82	o55	o55	NOUN
ajlm-609	125	83	)	)	PUNCT
ajlm-609	125	84	.	.	PUNCT
ajlm-609	126	1	serotypes	serotype	NOUN
ajlm-609	126	2	o26	o26	VERB
ajlm-609	126	3	and	and	CCONJ
ajlm-609	126	4	o104	o104	PROPN
ajlm-609	126	5	are	be	AUX
ajlm-609	126	6	among	among	ADP
ajlm-609	126	7	the	the	DET
ajlm-609	126	8	top	top	ADJ
ajlm-609	126	9	six	six	NUM
ajlm-609	126	10	stec	stec	ADJ
ajlm-609	126	11	serotypes	serotype	NOUN
ajlm-609	126	12	globally.25,26	globally.25,26	NOUN
ajlm-609	126	13	chromagartmstec	chromagartmstec	ADJ
ajlm-609	126	14	is	be	AUX
ajlm-609	126	15	intended	intend	VERB
ajlm-609	126	16	for	for	ADP
ajlm-609	126	17	stec	stec	ADJ
ajlm-609	126	18	culture	culture	NOUN
ajlm-609	126	19	;	;	PUNCT
ajlm-609	126	20	however	however	ADV
ajlm-609	126	21	,	,	PUNCT
ajlm-609	126	22	we	we	PRON
ajlm-609	126	23	detected	detect	VERB
ajlm-609	126	24	other	other	ADJ
ajlm-609	126	25	diarrhoeic	diarrhoeic	VERB
ajlm-609	126	26	e.	e.	PROPN
ajlm-609	126	27	coli	coli	PROPN
ajlm-609	126	28	pathotypes	pathotype	NOUN
ajlm-609	126	29	using	use	VERB
ajlm-609	126	30	this	this	DET
ajlm-609	126	31	medium	medium	NOUN
ajlm-609	126	32	.	.	PUNCT
ajlm-609	127	1	the	the	DET
ajlm-609	127	2	other	other	ADJ
ajlm-609	127	3	pathotypes	pathotype	NOUN
ajlm-609	127	4	detected	detect	VERB
ajlm-609	127	5	might	might	AUX
ajlm-609	127	6	have	have	AUX
ajlm-609	127	7	been	be	AUX
ajlm-609	127	8	hybrid	hybrid	VERB
ajlm-609	127	9	strains	strain	NOUN
ajlm-609	127	10	that	that	PRON
ajlm-609	127	11	lost	lose	VERB
ajlm-609	127	12	the	the	DET
ajlm-609	127	13	stx	stx	NOUN
ajlm-609	127	14	genes	gene	NOUN
ajlm-609	127	15	or	or	CCONJ
ajlm-609	127	16	hybrid	hybrid	NOUN
ajlm-609	127	17	strains	strain	NOUN
ajlm-609	127	18	whose	whose	DET
ajlm-609	127	19	stx	stx	NOUN
ajlm-609	127	20	genes	gene	NOUN
ajlm-609	127	21	could	could	AUX
ajlm-609	127	22	not	not	PART
ajlm-609	127	23	be	be	AUX
ajlm-609	127	24	detected	detect	VERB
ajlm-609	127	25	using	use	VERB
ajlm-609	127	26	the	the	DET
ajlm-609	127	27	primers	primer	NOUN
ajlm-609	127	28	employed	employ	VERB
ajlm-609	127	29	in	in	ADP
ajlm-609	127	30	this	this	DET
ajlm-609	127	31	study	study	NOUN
ajlm-609	127	32	.	.	PUNCT
ajlm-609	128	1	notably	notably	ADV
ajlm-609	128	2	,	,	PUNCT
ajlm-609	128	3	bacteriophages	bacteriophage	NOUN
ajlm-609	128	4	carrying	carry	VERB
ajlm-609	128	5	the	the	DET
ajlm-609	128	6	stx	stx	NOUN
ajlm-609	128	7	genes	gene	NOUN
ajlm-609	128	8	are	be	AUX
ajlm-609	128	9	very	very	ADV
ajlm-609	128	10	quickly	quickly	ADV
ajlm-609	128	11	lost	lose	VERB
ajlm-609	128	12	both	both	PRON
ajlm-609	128	13	in	in	ADP
ajlm-609	128	14	vivo	vivo	NOUN
ajlm-609	128	15	and	and	CCONJ
ajlm-609	128	16	in	in	ADP
ajlm-609	128	17	vitro,27	vitro,27	NUM
ajlm-609	128	18	and	and	CCONJ
ajlm-609	128	19	not	not	PART
ajlm-609	128	20	all	all	DET
ajlm-609	128	21	stx	stx	NOUN
ajlm-609	128	22	primers	primer	NOUN
ajlm-609	128	23	can	can	AUX
ajlm-609	128	24	detect	detect	VERB
ajlm-609	128	25	all	all	DET
ajlm-609	128	26	the	the	DET
ajlm-609	128	27	stx	stx	NOUN
ajlm-609	128	28	gene	gene	NOUN
ajlm-609	128	29	variants.28	variants.28	NOUN
ajlm-609	128	30	limitations	limitation	NOUN
ajlm-609	128	31	not	not	PART
ajlm-609	128	32	all	all	DET
ajlm-609	128	33	stec	stec	ADJ
ajlm-609	128	34	are	be	AUX
ajlm-609	128	35	tellurite	tellurite	NOUN
ajlm-609	128	36	resistant	resistant	ADJ
ajlm-609	128	37	and	and	CCONJ
ajlm-609	128	38	may	may	AUX
ajlm-609	128	39	have	have	AUX
ajlm-609	128	40	been	be	AUX
ajlm-609	128	41	missed	miss	VERB
ajlm-609	128	42	on	on	ADP
ajlm-609	128	43	chromagartmstec	chromagartmstec	ADJ
ajlm-609	128	44	culture	culture	NOUN
ajlm-609	128	45	.	.	PUNCT
ajlm-609	129	1	this	this	DET
ajlm-609	129	2	study	study	NOUN
ajlm-609	129	3	only	only	ADV
ajlm-609	129	4	focused	focus	VERB
ajlm-609	129	5	on	on	ADP
ajlm-609	129	6	the	the	DET
ajlm-609	129	7	strains	strain	NOUN
ajlm-609	129	8	that	that	PRON
ajlm-609	129	9	formed	form	VERB
ajlm-609	129	10	mauve	mauve	NOUN
ajlm-609	129	11	colonies	colony	NOUN
ajlm-609	129	12	.	.	PUNCT
ajlm-609	130	1	the	the	DET
ajlm-609	130	2	stool	stool	NOUN
ajlm-609	130	3	samples	sample	NOUN
ajlm-609	130	4	in	in	ADP
ajlm-609	130	5	this	this	DET
ajlm-609	130	6	study	study	NOUN
ajlm-609	130	7	were	be	AUX
ajlm-609	130	8	collected	collect	VERB
ajlm-609	130	9	between	between	ADP
ajlm-609	130	10	september	september	PROPN
ajlm-609	130	11	2014	2014	NUM
ajlm-609	130	12	and	and	CCONJ
ajlm-609	130	13	may	may	PROPN
ajlm-609	130	14	2015	2015	NUM
ajlm-609	130	15	,	,	PUNCT
ajlm-609	130	16	and	and	CCONJ
ajlm-609	130	17	therefore	therefore	ADV
ajlm-609	130	18	the	the	DET
ajlm-609	130	19	results	result	NOUN
ajlm-609	130	20	of	of	ADP
ajlm-609	130	21	this	this	DET
ajlm-609	130	22	study	study	NOUN
ajlm-609	130	23	as	as	SCONJ
ajlm-609	130	24	regards	regard	VERB
ajlm-609	130	25	prevalence	prevalence	NOUN
ajlm-609	130	26	of	of	ADP
ajlm-609	130	27	stec	stec	ADJ
ajlm-609	130	28	may	may	AUX
ajlm-609	130	29	not	not	PART
ajlm-609	130	30	reflect	reflect	VERB
ajlm-609	130	31	the	the	DET
ajlm-609	130	32	entire	entire	ADJ
ajlm-609	130	33	year	year	NOUN
ajlm-609	130	34	or	or	CCONJ
ajlm-609	130	35	areas	area	NOUN
ajlm-609	130	36	of	of	ADP
ajlm-609	130	37	south	south	PROPN
ajlm-609	130	38	africa	africa	PROPN
ajlm-609	130	39	other	other	ADJ
ajlm-609	130	40	than	than	ADP
ajlm-609	130	41	cape	cape	NOUN
ajlm-609	130	42	town	town	NOUN
ajlm-609	130	43	.	.	PUNCT
ajlm-609	131	1	conclusions	conclusion	NOUN
ajlm-609	131	2	the	the	DET
ajlm-609	131	3	in	in	ADP
ajlm-609	131	4	-	-	PUNCT
ajlm-609	131	5	house	house	NOUN
ajlm-609	131	6	developed	develop	VERB
ajlm-609	131	7	real	real	ADJ
ajlm-609	131	8	-	-	PUNCT
ajlm-609	131	9	time	time	NOUN
ajlm-609	131	10	pcr	pcr	PROPN
ajlm-609	131	11	assay	assay	NOUN
ajlm-609	131	12	is	be	AUX
ajlm-609	131	13	a	a	DET
ajlm-609	131	14	sensitive	sensitive	ADJ
ajlm-609	131	15	and	and	CCONJ
ajlm-609	131	16	specific	specific	ADJ
ajlm-609	131	17	alternative	alternative	NOUN
ajlm-609	131	18	to	to	ADP
ajlm-609	131	19	the	the	DET
ajlm-609	131	20	currently	currently	ADV
ajlm-609	131	21	used	use	VERB
ajlm-609	131	22	diagnostic	diagnostic	ADJ
ajlm-609	131	23	strategy	strategy	NOUN
ajlm-609	131	24	.	.	PUNCT
ajlm-609	132	1	due	due	ADP
ajlm-609	132	2	to	to	ADP
ajlm-609	132	3	the	the	DET
ajlm-609	132	4	high	high	ADJ
ajlm-609	132	5	false	false	ADJ
ajlm-609	132	6	positivity	positivity	NOUN
ajlm-609	132	7	rate	rate	NOUN
ajlm-609	132	8	,	,	PUNCT
ajlm-609	132	9	chromagartmstec	chromagartmstec	ADJ
ajlm-609	132	10	can	can	AUX
ajlm-609	132	11	only	only	ADV
ajlm-609	132	12	be	be	AUX
ajlm-609	132	13	used	use	VERB
ajlm-609	132	14	as	as	ADP
ajlm-609	132	15	an	an	DET
ajlm-609	132	16	adjunt	adjunt	NOUN
ajlm-609	132	17	to	to	ADP
ajlm-609	132	18	a	a	DET
ajlm-609	132	19	more	more	ADV
ajlm-609	132	20	sensitive	sensitive	ADJ
ajlm-609	132	21	and	and	CCONJ
ajlm-609	132	22	specific	specific	ADJ
ajlm-609	132	23	assay	assay	NOUN
ajlm-609	132	24	such	such	ADJ
ajlm-609	132	25	as	as	ADP
ajlm-609	132	26	real	real	ADJ
ajlm-609	132	27	-	-	PUNCT
ajlm-609	132	28	time	time	NOUN
ajlm-609	132	29	pcr	pcr	PROPN
ajlm-609	132	30	.	.	PUNCT
ajlm-609	133	1	trustworthiness	trustworthiness	NOUN
ajlm-609	133	2	to	to	ADP
ajlm-609	133	3	the	the	DET
ajlm-609	133	4	best	good	ADJ
ajlm-609	133	5	of	of	ADP
ajlm-609	133	6	our	our	PRON
ajlm-609	133	7	knowledge	knowledge	NOUN
ajlm-609	133	8	,	,	PUNCT
ajlm-609	133	9	the	the	DET
ajlm-609	133	10	findings	finding	NOUN
ajlm-609	133	11	of	of	ADP
ajlm-609	133	12	this	this	DET
ajlm-609	133	13	study	study	NOUN
ajlm-609	133	14	can	can	AUX
ajlm-609	133	15	be	be	AUX
ajlm-609	133	16	used	use	VERB
ajlm-609	133	17	as	as	ADP
ajlm-609	133	18	per	per	ADP
ajlm-609	133	19	the	the	DET
ajlm-609	133	20	scope	scope	NOUN
ajlm-609	133	21	of	of	ADP
ajlm-609	133	22	the	the	DET
ajlm-609	133	23	study	study	NOUN
ajlm-609	133	24	and	and	CCONJ
ajlm-609	133	25	in	in	ADP
ajlm-609	133	26	light	light	NOUN
ajlm-609	133	27	of	of	ADP
ajlm-609	133	28	the	the	DET
ajlm-609	133	29	study	study	NOUN
ajlm-609	133	30	limitations	limitation	NOUN
ajlm-609	133	31	as	as	SCONJ
ajlm-609	133	32	clearly	clearly	ADV
ajlm-609	133	33	pointed	point	VERB
ajlm-609	133	34	out	out	ADP
ajlm-609	133	35	.	.	PUNCT
ajlm-609	134	1	reliability	reliability	NOUN
ajlm-609	134	2	we	we	PRON
ajlm-609	134	3	confirm	confirm	VERB
ajlm-609	134	4	that	that	SCONJ
ajlm-609	134	5	the	the	DET
ajlm-609	134	6	experiments	experiment	NOUN
ajlm-609	134	7	conducted	conduct	VERB
ajlm-609	134	8	in	in	ADP
ajlm-609	134	9	this	this	DET
ajlm-609	134	10	study	study	NOUN
ajlm-609	134	11	will	will	AUX
ajlm-609	134	12	yield	yield	VERB
ajlm-609	134	13	the	the	DET
ajlm-609	134	14	same	same	ADJ
ajlm-609	134	15	results	result	NOUN
ajlm-609	134	16	during	during	ADP
ajlm-609	134	17	repeated	repeat	VERB
ajlm-609	134	18	trials	trial	NOUN
ajlm-609	134	19	using	use	VERB
ajlm-609	134	20	the	the	DET
ajlm-609	134	21	same	same	ADJ
ajlm-609	134	22	reagents	reagent	NOUN
ajlm-609	134	23	and	and	CCONJ
ajlm-609	134	24	detection	detection	NOUN
ajlm-609	134	25	platforms	platform	NOUN
ajlm-609	134	26	.	.	PUNCT
ajlm-609	135	1	validity	validity	NOUN
ajlm-609	135	2	to	to	ADP
ajlm-609	135	3	the	the	DET
ajlm-609	135	4	best	good	ADJ
ajlm-609	135	5	of	of	ADP
ajlm-609	135	6	our	our	PRON
ajlm-609	135	7	knowledge	knowledge	NOUN
ajlm-609	135	8	,	,	PUNCT
ajlm-609	135	9	the	the	DET
ajlm-609	135	10	findings	finding	NOUN
ajlm-609	135	11	of	of	ADP
ajlm-609	135	12	this	this	DET
ajlm-609	135	13	study	study	NOUN
ajlm-609	135	14	,	,	PUNCT
ajlm-609	135	15	as	as	SCONJ
ajlm-609	135	16	obtained	obtain	VERB
ajlm-609	135	17	using	use	VERB
ajlm-609	135	18	the	the	DET
ajlm-609	135	19	methods	method	NOUN
ajlm-609	135	20	we	we	PRON
ajlm-609	135	21	employed	employ	VERB
ajlm-609	135	22	,	,	PUNCT
ajlm-609	135	23	are	be	AUX
ajlm-609	135	24	valid	valid	ADJ
ajlm-609	135	25	for	for	ADP
ajlm-609	135	26	the	the	DET
ajlm-609	135	27	study	study	NOUN
ajlm-609	135	28	area	area	NOUN
ajlm-609	135	29	and	and	CCONJ
ajlm-609	135	30	season	season	NOUN
ajlm-609	135	31	.	.	PUNCT
ajlm-609	136	1	the	the	DET
ajlm-609	136	2	in	in	ADP
ajlm-609	136	3	-	-	PUNCT
ajlm-609	136	4	house	house	NOUN
ajlm-609	136	5	developed	develop	VERB
ajlm-609	136	6	real	real	ADJ
ajlm-609	136	7	-	-	PUNCT
ajlm-609	136	8	time	time	NOUN
ajlm-609	136	9	pcr	pcr	NOUN
ajlm-609	136	10	may	may	AUX
ajlm-609	136	11	,	,	PUNCT
ajlm-609	136	12	however	however	ADV
ajlm-609	136	13	,	,	PUNCT
ajlm-609	136	14	be	be	AUX
ajlm-609	136	15	adopted	adopt	VERB
ajlm-609	136	16	in	in	ADP
ajlm-609	136	17	other	other	ADJ
ajlm-609	136	18	laboratories	laboratory	NOUN
ajlm-609	136	19	in	in	ADP
ajlm-609	136	20	developing	develop	VERB
ajlm-609	136	21	countries	country	NOUN
ajlm-609	136	22	.	.	PUNCT
ajlm-609	137	1	acknowledgements	acknowledgement	NOUN
ajlm-609	137	2	we	we	PRON
ajlm-609	137	3	acknowledge	acknowledge	VERB
ajlm-609	137	4	the	the	DET
ajlm-609	137	5	center	center	NOUN
ajlm-609	137	6	for	for	ADP
ajlm-609	137	7	enteric	enteric	ADJ
ajlm-609	137	8	disease	disease	NOUN
ajlm-609	137	9	research	research	NOUN
ajlm-609	137	10	unit	unit	NOUN
ajlm-609	137	11	,	,	PUNCT
ajlm-609	137	12	national	national	PROPN
ajlm-609	137	13	institute	institute	PROPN
ajlm-609	137	14	of	of	ADP
ajlm-609	137	15	communicable	communicable	NOUN
ajlm-609	137	16	disease	disease	NOUN
ajlm-609	137	17	(	(	PUNCT
ajlm-609	137	18	sandringham	sandringham	PROPN
ajlm-609	137	19	,	,	PUNCT
ajlm-609	137	20	johannesburg	johannesburg	PROPN
ajlm-609	137	21	,	,	PUNCT
ajlm-609	137	22	south	south	PROPN
ajlm-609	137	23	africa	africa	PROPN
ajlm-609	137	24	)	)	PUNCT
ajlm-609	137	25	for	for	ADP
ajlm-609	137	26	serotyping	serotyping	NOUN
ajlm-609	137	27	of	of	ADP
ajlm-609	137	28	the	the	DET
ajlm-609	137	29	e.	e.	PROPN
ajlm-609	137	30	coli	coli	PROPN
ajlm-609	137	31	isolates	isolate	NOUN
ajlm-609	137	32	.	.	PUNCT
ajlm-609	138	1	we	we	PRON
ajlm-609	138	2	also	also	ADV
ajlm-609	138	3	acknowledge	acknowledge	VERB
ajlm-609	138	4	the	the	DET
ajlm-609	138	5	national	national	PROPN
ajlm-609	138	6	research	research	PROPN
ajlm-609	138	7	foundation	foundation	PROPN
ajlm-609	138	8	,	,	PUNCT
ajlm-609	138	9	south	south	PROPN
ajlm-609	138	10	africa	africa	PROPN
ajlm-609	138	11	,	,	PUNCT
ajlm-609	138	12	the	the	DET
ajlm-609	138	13	acp	acp	PROPN
ajlm-609	138	14	-	-	PUNCT
ajlm-609	138	15	rise	rise	NOUN
ajlm-609	138	16	scholarship	scholarship	NOUN
ajlm-609	138	17	fund	fund	NOUN
ajlm-609	138	18	and	and	CCONJ
ajlm-609	138	19	the	the	DET
ajlm-609	138	20	adb	adb	PROPN
ajlm-609	138	21	-	-	PUNCT
ajlm-609	138	22	hest	h	ADJ
ajlm-609	138	23	fund	fund	NOUN
ajlm-609	138	24	.	.	PUNCT
ajlm-609	139	1	competing	compete	VERB
ajlm-609	139	2	interests	interest	NOUN
ajlm-609	139	3	the	the	DET
ajlm-609	139	4	authors	author	NOUN
ajlm-609	139	5	declare	declare	VERB
ajlm-609	139	6	that	that	SCONJ
ajlm-609	139	7	there	there	PRON
ajlm-609	139	8	are	be	VERB
ajlm-609	139	9	no	no	DET
ajlm-609	139	10	financial	financial	ADJ
ajlm-609	139	11	or	or	CCONJ
ajlm-609	139	12	personal	personal	ADJ
ajlm-609	139	13	relationships	relationship	NOUN
ajlm-609	139	14	that	that	PRON
ajlm-609	139	15	may	may	AUX
ajlm-609	139	16	have	have	AUX
ajlm-609	139	17	had	have	VERB
ajlm-609	139	18	influence	influence	NOUN
ajlm-609	139	19	in	in	ADP
ajlm-609	139	20	the	the	DET
ajlm-609	139	21	writing	writing	NOUN
ajlm-609	139	22	of	of	ADP
ajlm-609	139	23	this	this	DET
ajlm-609	139	24	article	article	NOUN
ajlm-609	139	25	.	.	PUNCT
ajlm-609	140	1	sources	source	NOUN
ajlm-609	140	2	of	of	ADP
ajlm-609	140	3	support	support	NOUN
ajlm-609	140	4	funds	fund	NOUN
ajlm-609	140	5	for	for	ADP
ajlm-609	140	6	this	this	DET
ajlm-609	140	7	study	study	NOUN
ajlm-609	140	8	were	be	AUX
ajlm-609	140	9	provided	provide	VERB
ajlm-609	140	10	by	by	ADP
ajlm-609	140	11	the	the	DET
ajlm-609	140	12	national	national	PROPN
ajlm-609	140	13	research	research	PROPN
ajlm-609	140	14	foundation	foundation	PROPN
ajlm-609	140	15	,	,	PUNCT
ajlm-609	140	16	south	south	PROPN
ajlm-609	140	17	africa	africa	PROPN
ajlm-609	140	18	,	,	PUNCT
ajlm-609	140	19	the	the	DET
ajlm-609	140	20	adb	adb	PROPN
ajlm-609	140	21	-	-	PUNCT
ajlm-609	140	22	hest	h	ADJ
ajlm-609	140	23	scholarship	scholarship	NOUN
ajlm-609	140	24	fund	fund	NOUN
ajlm-609	140	25	and	and	CCONJ
ajlm-609	140	26	the	the	DET
ajlm-609	140	27	acp	acp	PROPN
ajlm-609	140	28	-	-	PUNCT
ajlm-609	140	29	rise	rise	NOUN
ajlm-609	140	30	scholarship	scholarship	NOUN
ajlm-609	140	31	fund	fund	NOUN
ajlm-609	140	32	.	.	PUNCT
ajlm-609	141	1	authors	author	NOUN
ajlm-609	141	2	’	’	PART
ajlm-609	141	3	contributions	contribution	NOUN
ajlm-609	141	4	j.b.k	j.b.k	VERB
ajlm-609	141	5	.	.	PUNCT
ajlm-609	141	6	participated	participate	VERB
ajlm-609	141	7	in	in	ADP
ajlm-609	141	8	processing	process	VERB
ajlm-609	141	9	the	the	DET
ajlm-609	141	10	samples	sample	NOUN
ajlm-609	141	11	,	,	PUNCT
ajlm-609	141	12	data	datum	NOUN
ajlm-609	141	13	analysis	analysis	NOUN
ajlm-609	141	14	and	and	CCONJ
ajlm-609	141	15	writing	writing	NOUN
ajlm-609	141	16	of	of	ADP
ajlm-609	141	17	the	the	DET
ajlm-609	141	18	manuscript	manuscript	NOUN
ajlm-609	141	19	.	.	PUNCT
ajlm-609	142	1	k.h.k	k.h.k	PROPN
ajlm-609	142	2	.	.	PROPN
ajlm-609	142	3	participated	participate	VERB
ajlm-609	142	4	in	in	ADP
ajlm-609	142	5	the	the	DET
ajlm-609	142	6	supervision	supervision	NOUN
ajlm-609	142	7	of	of	ADP
ajlm-609	142	8	the	the	DET
ajlm-609	142	9	work	work	NOUN
ajlm-609	142	10	,	,	PUNCT
ajlm-609	142	11	development	development	NOUN
ajlm-609	142	12	of	of	ADP
ajlm-609	142	13	the	the	DET
ajlm-609	142	14	idea	idea	NOUN
ajlm-609	142	15	and	and	CCONJ
ajlm-609	142	16	writing	writing	NOUN
ajlm-609	142	17	of	of	ADP
ajlm-609	142	18	the	the	DET
ajlm-609	142	19	manuscript	manuscript	NOUN
ajlm-609	142	20	.	.	PUNCT
ajlm-609	143	1	a.s	a.s	PROPN
ajlm-609	143	2	.	.	PROPN
ajlm-609	143	3	participated	participate	VERB
ajlm-609	143	4	in	in	ADP
ajlm-609	143	5	the	the	DET
ajlm-609	143	6	writing	writing	NOUN
ajlm-609	143	7	of	of	ADP
ajlm-609	143	8	the	the	DET
ajlm-609	143	9	manuscript	manuscript	NOUN
ajlm-609	143	10	and	and	CCONJ
ajlm-609	143	11	supervision	supervision	NOUN
ajlm-609	143	12	of	of	ADP
ajlm-609	143	13	the	the	DET
ajlm-609	143	14	work	work	NOUN
ajlm-609	143	15	.	.	PUNCT
ajlm-609	144	1	m.p.n	m.p.n	VERB
ajlm-609	144	2	.	.	PROPN
ajlm-609	144	3	participated	participate	VERB
ajlm-609	144	4	in	in	ADP
ajlm-609	144	5	the	the	DET
ajlm-609	144	6	development	development	NOUN
ajlm-609	144	7	of	of	ADP
ajlm-609	144	8	the	the	DET
ajlm-609	144	9	idea	idea	NOUN
ajlm-609	144	10	,	,	PUNCT
ajlm-609	144	11	supervision	supervision	NOUN
ajlm-609	144	12	of	of	ADP
ajlm-609	144	13	the	the	DET
ajlm-609	144	14	work	work	NOUN
ajlm-609	144	15	and	and	CCONJ
ajlm-609	144	16	writing	writing	NOUN
ajlm-609	144	17	of	of	ADP
ajlm-609	144	18	the	the	DET
ajlm-609	144	19	manuscript	manuscript	NOUN
ajlm-609	144	20	.	.	PUNCT
ajlm-609	145	1	l.r	l.r	PROPN
ajlm-609	145	2	.	.	PROPN
ajlm-609	145	3	developed	develop	VERB
ajlm-609	145	4	the	the	DET
ajlm-609	145	5	idea	idea	NOUN
ajlm-609	145	6	,	,	PUNCT
ajlm-609	145	7	participated	participate	VERB
ajlm-609	145	8	in	in	ADP
ajlm-609	145	9	supervision	supervision	NOUN
ajlm-609	145	10	and	and	CCONJ
ajlm-609	145	11	in	in	ADP
ajlm-609	145	12	writing	writing	NOUN
ajlm-609	145	13	of	of	ADP
ajlm-609	145	14	the	the	DET
ajlm-609	145	15	manuscript	manuscript	NOUN
ajlm-609	145	16	.	.	PUNCT
ajlm-609	146	1	references	reference	NOUN
ajlm-609	146	2	slayton	slayton	PROPN
ajlm-609	146	3	rb	rb	PROPN
ajlm-609	146	4	,	,	PUNCT
ajlm-609	146	5	turabelidze	turabelidze	NOUN
ajlm-609	146	6	g	g	PROPN
ajlm-609	146	7	,	,	PUNCT
ajlm-609	146	8	bennett	bennett	PROPN
ajlm-609	146	9	sd	sd	PROPN
ajlm-609	146	10	,	,	PUNCT
ajlm-609	146	11	et	et	PROPN
ajlm-609	146	12	al	al	PROPN
ajlm-609	146	13	.	.	PROPN
ajlm-609	146	14	outbreak	outbreak	PROPN
ajlm-609	146	15	of	of	ADP
ajlm-609	146	16	shiga	shiga	ADJ
ajlm-609	146	17	toxin	toxin	NOUN
ajlm-609	146	18	-	-	PUNCT
ajlm-609	146	19	producing	produce	VERB
ajlm-609	146	20	escherichia	escherichia	NOUN
ajlm-609	146	21	coli	coli	NOUN
ajlm-609	146	22	(	(	PUNCT
ajlm-609	146	23	stec	stec	ADJ
ajlm-609	146	24	)	)	PUNCT
ajlm-609	146	25	o157	o157	PROPN
ajlm-609	146	26	:	:	PUNCT
ajlm-609	147	1	h7	h7	PROPN
ajlm-609	147	2	associated	associate	VERB
ajlm-609	147	3	with	with	ADP
ajlm-609	147	4	romaine	romaine	NOUN
ajlm-609	147	5	lettuce	lettuce	NOUN
ajlm-609	147	6	consumption	consumption	NOUN
ajlm-609	147	7	,	,	PUNCT
ajlm-609	147	8	2011	2011	NUM
ajlm-609	147	9	.	.	PUNCT
ajlm-609	148	1	plos	plos	PROPN
ajlm-609	148	2	one	one	NUM
ajlm-609	148	3	.	.	PUNCT
ajlm-609	149	1	2013;8(2):e55300	2013;8(2):e55300	NUM
ajlm-609	149	2	.	.	PUNCT
ajlm-609	150	1	https://doi.org/10.1371/journal.pone.0055300	https://doi.org/10.1371/journal.pone.0055300	VERB
ajlm-609	150	2	friesema	friesema	ADP
ajlm-609	151	1	i	i	PRON
ajlm-609	151	2	,	,	PUNCT
ajlm-609	151	3	schimmer	schimmer	PROPN
ajlm-609	151	4	b	b	PROPN
ajlm-609	151	5	,	,	PUNCT
ajlm-609	151	6	stenvers	stenver	NOUN
ajlm-609	151	7	o	o	NOUN
ajlm-609	151	8	,	,	PUNCT
ajlm-609	151	9	et	et	PROPN
ajlm-609	151	10	al	al	PROPN
ajlm-609	151	11	.	.	PROPN
ajlm-609	151	12	stec	stec	PROPN
ajlm-609	151	13	o157	o157	PROPN
ajlm-609	151	14	outbreak	outbreak	NOUN
ajlm-609	151	15	in	in	ADP
ajlm-609	151	16	the	the	DET
ajlm-609	151	17	netherlands	netherlands	PROPN
ajlm-609	151	18	,	,	PUNCT
ajlm-609	151	19	september	september	PROPN
ajlm-609	151	20	–	–	PUNCT
ajlm-609	151	21	october	october	PROPN
ajlm-609	151	22	2007	2007	NUM
ajlm-609	151	23	.	.	PUNCT
ajlm-609	152	1	euro	euro	NOUN
ajlm-609	152	2	surveill	surveill	NOUN
ajlm-609	152	3	.	.	PUNCT
ajlm-609	153	1	2007;12(11):e071101	2007;12(11):e071101	NUM
ajlm-609	153	2	1	1	NUM
ajlm-609	153	3	.	.	PUNCT
ajlm-609	153	4	heiman	heiman	NOUN
ajlm-609	153	5	ke	ke	PROPN
ajlm-609	153	6	,	,	PUNCT
ajlm-609	153	7	mody	mody	PROPN
ajlm-609	153	8	rk	rk	NOUN
ajlm-609	153	9	,	,	PUNCT
ajlm-609	153	10	johnson	johnson	PROPN
ajlm-609	153	11	sd	sd	PROPN
ajlm-609	153	12	,	,	PUNCT
ajlm-609	153	13	et	et	PROPN
ajlm-609	153	14	al	al	PROPN
ajlm-609	153	15	.	.	PROPN
ajlm-609	153	16	escherichia	escherichia	NOUN
ajlm-609	153	17	coli	coli	NOUN
ajlm-609	153	18	o157	o157	PROPN
ajlm-609	153	19	outbreaks	outbreak	NOUN
ajlm-609	153	20	in	in	ADP
ajlm-609	153	21	the	the	DET
ajlm-609	153	22	united	united	PROPN
ajlm-609	153	23	states	states	PROPN
ajlm-609	153	24	,	,	PUNCT
ajlm-609	153	25	2003–2012	2003–2012	NUM
ajlm-609	153	26	.	.	PUNCT
ajlm-609	154	1	emerg	emerg	PROPN
ajlm-609	154	2	infect	infect	PROPN
ajlm-609	154	3	dis	dis	PROPN
ajlm-609	154	4	.	.	PUNCT
ajlm-609	155	1	2015;21(8):1293–1301	2015;21(8):1293–1301	NUM
ajlm-609	155	2	.	.	PUNCT
ajlm-609	156	1	https://doi.org/10.3201/eid2108.141364	https://doi.org/10.3201/eid2108.141364	PUNCT
ajlm-609	156	2	luna	luna	PROPN
ajlm-609	156	3	-	-	PUNCT
ajlm-609	156	4	gierke	gierke	PROPN
ajlm-609	156	5	re	re	PROPN
ajlm-609	156	6	,	,	PUNCT
ajlm-609	156	7	griffin	griffin	PROPN
ajlm-609	156	8	pm	pm	PROPN
ajlm-609	156	9	,	,	PUNCT
ajlm-609	156	10	gould	gould	PROPN
ajlm-609	156	11	lh	lh	PROPN
ajlm-609	156	12	,	,	PUNCT
ajlm-609	156	13	et	et	PROPN
ajlm-609	156	14	al	al	PROPN
ajlm-609	156	15	.	.	PUNCT
ajlm-609	157	1	outbreaks	outbreak	NOUN
ajlm-609	157	2	of	of	ADP
ajlm-609	157	3	non	non	ADJ
ajlm-609	157	4	-	-	ADJ
ajlm-609	157	5	o157	o157	ADJ
ajlm-609	157	6	shiga	shiga	ADJ
ajlm-609	157	7	toxin	toxin	NOUN
ajlm-609	157	8	-	-	PUNCT
ajlm-609	157	9	producing	produce	VERB
ajlm-609	157	10	escherichia	escherichia	NOUN
ajlm-609	157	11	coli	coli	NOUN
ajlm-609	157	12	infection	infection	NOUN
ajlm-609	157	13	:	:	PUNCT
ajlm-609	157	14	usa	usa	PROPN
ajlm-609	157	15	.	.	PROPN
ajlm-609	157	16	epidemiol	epidemiol	PROPN
ajlm-609	157	17	infect	infect	PROPN
ajlm-609	157	18	.	.	PUNCT
ajlm-609	158	1	2014;142(11):2270–2280	2014;142(11):2270–2280	NUM
ajlm-609	158	2	.	.	PUNCT
ajlm-609	159	1	https://doi.org/10.1017/s0950268813003233	https://doi.org/10.1017/s0950268813003233	NUM
ajlm-609	159	2	gould	gould	PROPN
ajlm-609	159	3	lh	lh	PROPN
ajlm-609	159	4	.	.	PUNCT
ajlm-609	160	1	recommendations	recommendation	NOUN
ajlm-609	160	2	for	for	ADP
ajlm-609	160	3	diagnosis	diagnosis	NOUN
ajlm-609	160	4	of	of	ADP
ajlm-609	160	5	shiga	shiga	ADJ
ajlm-609	160	6	toxin	toxin	NOUN
ajlm-609	160	7	producing	produce	VERB
ajlm-609	160	8	escherichia	escherichia	NOUN
ajlm-609	160	9	coli	coli	NOUN
ajlm-609	160	10	infections	infection	NOUN
ajlm-609	160	11	by	by	ADP
ajlm-609	160	12	clinical	clinical	ADJ
ajlm-609	160	13	laboratories	laboratory	NOUN
ajlm-609	160	14	.	.	PUNCT
ajlm-609	161	1	wkly	wkly	PROPN
ajlm-609	161	2	rep	rep	PROPN
ajlm-609	161	3	.	.	PROPN
ajlm-609	161	4	2009;58:1e14	2009;58:1e14	PROPN
ajlm-609	161	5	.	.	PROPN
ajlm-609	161	6	https://doi.org/10.1016/j.clinmicnews.2012.04.004	https://doi.org/10.1016/j.clinmicnews.2012.04.004	PROPN
ajlm-609	161	7	germani	germani	PROPN
ajlm-609	161	8	y	y	PROPN
ajlm-609	161	9	,	,	PUNCT
ajlm-609	161	10	soro	soro	PROPN
ajlm-609	161	11	b	b	PROPN
ajlm-609	161	12	,	,	PUNCT
ajlm-609	161	13	vohito	vohito	NOUN
ajlm-609	161	14	m	m	PROPN
ajlm-609	161	15	,	,	PUNCT
ajlm-609	161	16	et	et	PROPN
ajlm-609	161	17	al	al	PROPN
ajlm-609	161	18	.	.	PROPN
ajlm-609	161	19	enterohaemorrhagic	enterohaemorrhagic	PROPN
ajlm-609	161	20	escherichia	escherichia	NOUN
ajlm-609	161	21	coli	coli	NOUN
ajlm-609	161	22	in	in	ADP
ajlm-609	161	23	central	central	ADJ
ajlm-609	161	24	african	african	ADJ
ajlm-609	161	25	republic	republic	NOUN
ajlm-609	161	26	.	.	PUNCT
ajlm-609	162	1	lancet	lancet	PROPN
ajlm-609	162	2	.	.	PUNCT
ajlm-609	163	1	1997;349(9066):1670	1997;349(9066):1670	X
ajlm-609	163	2	.	.	PUNCT
ajlm-609	164	1	https://doi.org/10.1016/s0140-6736(05)62636-0	https://doi.org/10.1016/s0140-6736(05)62636-0	PROPN
ajlm-609	164	2	germani	germani	PROPN
ajlm-609	164	3	y	y	PROPN
ajlm-609	164	4	,	,	PUNCT
ajlm-609	164	5	cunin	cunin	NOUN
ajlm-609	164	6	p	p	NOUN
ajlm-609	164	7	,	,	PUNCT
ajlm-609	164	8	tedjouka	tedjouka	PROPN
ajlm-609	164	9	e	e	NOUN
ajlm-609	164	10	,	,	PUNCT
ajlm-609	164	11	et	et	PROPN
ajlm-609	164	12	al	al	PROPN
ajlm-609	164	13	.	.	PROPN
ajlm-609	164	14	enterohaemorrhagic	enterohaemorrhagic	PROPN
ajlm-609	164	15	escherichia	escherichia	NOUN
ajlm-609	164	16	coli	coli	NOUN
ajlm-609	164	17	in	in	ADP
ajlm-609	164	18	ngoila	ngoila	PROPN
ajlm-609	164	19	(	(	PUNCT
ajlm-609	164	20	cameroon	cameroon	NOUN
ajlm-609	164	21	)	)	PUNCT
ajlm-609	164	22	during	during	ADP
ajlm-609	164	23	an	an	DET
ajlm-609	164	24	outbreak	outbreak	NOUN
ajlm-609	164	25	of	of	ADP
ajlm-609	164	26	bloody	bloody	ADJ
ajlm-609	164	27	diarrhoea	diarrhoea	NOUN
ajlm-609	164	28	.	.	PUNCT
ajlm-609	165	1	lancet	lancet	PROPN
ajlm-609	165	2	.	.	PUNCT
ajlm-609	166	1	1998;352(9128):625–626	1998;352(9128):625–626	X
ajlm-609	166	2	.	.	PUNCT
ajlm-609	167	1	https://doi.org/10.1016/s0140-6736(05)62636-0	https://doi.org/10.1016/s0140-6736(05)62636-0	PROPN
ajlm-609	167	2	koyange	koyange	PROPN
ajlm-609	167	3	l	l	NOUN
ajlm-609	167	4	,	,	PUNCT
ajlm-609	167	5	ollivier	ollivi	ADJ
ajlm-609	167	6	g	g	NOUN
ajlm-609	167	7	,	,	PUNCT
ajlm-609	167	8	muyembe	muyembe	PROPN
ajlm-609	167	9	jj	jj	PROPN
ajlm-609	167	10	,	,	PUNCT
ajlm-609	167	11	et	et	PROPN
ajlm-609	167	12	al	al	PROPN
ajlm-609	167	13	.	.	PROPN
ajlm-609	167	14	enterohemorrhagic	enterohemorrhagic	PROPN
ajlm-609	167	15	escherichia	escherichia	NOUN
ajlm-609	167	16	coli	coli	NOUN
ajlm-609	167	17	o157	o157	PROPN
ajlm-609	167	18	,	,	PUNCT
ajlm-609	167	19	kinshasa	kinshasa	PROPN
ajlm-609	167	20	.	.	PUNCT
ajlm-609	168	1	emerg	emerg	PROPN
ajlm-609	168	2	infect	infect	PROPN
ajlm-609	168	3	dis	dis	PROPN
ajlm-609	168	4	.	.	PUNCT
ajlm-609	169	1	2004;10(5):968–969	2004;10(5):968–969	X
ajlm-609	169	2	.	.	PUNCT
ajlm-609	169	3	https://doi.org/10.3201/eid1005.031034	https://doi.org/10.3201/eid1005.031034	PROPN
ajlm-609	170	1	soares	soares	PROPN
ajlm-609	170	2	be	be	VERB
ajlm-609	170	3	.	.	PUNCT
ajlm-609	171	1	quality	quality	NOUN
ajlm-609	171	2	of	of	ADP
ajlm-609	171	3	medical	medical	ADJ
ajlm-609	171	4	laboratory	laboratory	NOUN
ajlm-609	171	5	services	service	NOUN
ajlm-609	171	6	in	in	ADP
ajlm-609	171	7	resource	resource	NOUN
ajlm-609	171	8	-	-	PUNCT
ajlm-609	171	9	limited	limit	VERB
ajlm-609	171	10	settings	setting	NOUN
ajlm-609	171	11	.	.	PUNCT
ajlm-609	172	1	afr	afr	PROPN
ajlm-609	172	2	j	j	PROPN
ajlm-609	172	3	infect	infect	PROPN
ajlm-609	172	4	dis	dis	PROPN
ajlm-609	172	5	.	.	PUNCT
ajlm-609	173	1	2012;6(1):10–11	2012;6(1):10–11	NUM
ajlm-609	173	2	.	.	PUNCT
ajlm-609	174	1	https://doi.org/10.4314/ajid.v6i1.77736	https://doi.org/10.4314/ajid.v6i1.77736	NOUN
ajlm-609	174	2	loefler	loefler	NOUN
ajlm-609	174	3	ij	ij	NOUN
ajlm-609	174	4	.	.	PUNCT
ajlm-609	175	1	surgical	surgical	ADJ
ajlm-609	175	2	wound	wound	NOUN
ajlm-609	175	3	infection	infection	NOUN
ajlm-609	175	4	in	in	ADP
ajlm-609	175	5	the	the	DET
ajlm-609	175	6	third	third	ADJ
ajlm-609	175	7	world	world	NOUN
ajlm-609	175	8	:	:	PUNCT
ajlm-609	175	9	the	the	DET
ajlm-609	175	10	african	african	ADJ
ajlm-609	175	11	experience	experience	NOUN
ajlm-609	175	12	.	.	PUNCT
ajlm-609	176	1	j	j	PROPN
ajlm-609	176	2	med	med	PROPN
ajlm-609	176	3	microbiol	microbiol	PROPN
ajlm-609	176	4	.	.	PUNCT
ajlm-609	177	1	1998;47(6):471–473	1998;47(6):471–473	NUM
ajlm-609	177	2	.	.	PUNCT
ajlm-609	177	3	https://doi.org/10.1099/00222615-47-6-471	https://doi.org/10.1099/00222615-47-6-471	NOUN
ajlm-609	178	1	van	van	PROPN
ajlm-609	178	2	biljon	biljon	NOUN
ajlm-609	178	3	g.	g.	PROPN
ajlm-609	178	4	causes	cause	NOUN
ajlm-609	178	5	,	,	PUNCT
ajlm-609	178	6	prognostic	prognostic	ADJ
ajlm-609	178	7	factors	factor	NOUN
ajlm-609	178	8	and	and	CCONJ
ajlm-609	178	9	treatment	treatment	NOUN
ajlm-609	178	10	results	result	NOUN
ajlm-609	178	11	of	of	ADP
ajlm-609	178	12	acute	acute	ADJ
ajlm-609	178	13	renal	renal	ADJ
ajlm-609	178	14	failure	failure	NOUN
ajlm-609	178	15	in	in	ADP
ajlm-609	178	16	children	child	NOUN
ajlm-609	178	17	treated	treat	VERB
ajlm-609	178	18	in	in	ADP
ajlm-609	178	19	a	a	DET
ajlm-609	178	20	tertiary	tertiary	ADJ
ajlm-609	178	21	hospital	hospital	NOUN
ajlm-609	178	22	in	in	ADP
ajlm-609	178	23	south	south	PROPN
ajlm-609	178	24	africa	africa	PROPN
ajlm-609	178	25	.	.	PUNCT
ajlm-609	179	1	j	j	PROPN
ajlm-609	179	2	trop	trop	X
ajlm-609	179	3	pediatr	pediatr	PROPN
ajlm-609	179	4	.	.	PUNCT
ajlm-609	180	1	2008;54(4):233–237	2008;54(4):233–237	NUM
ajlm-609	180	2	.	.	PUNCT
ajlm-609	181	1	https://doi.org/10.1093/tropej/fmm079	https://doi.org/10.1093/tropej/fmm079	PROPN
ajlm-609	181	2	gouali	gouali	PROPN
ajlm-609	181	3	m	m	NOUN
ajlm-609	181	4	,	,	PUNCT
ajlm-609	181	5	ruckly	ruckly	NOUN
ajlm-609	181	6	c	c	NOUN
ajlm-609	181	7	,	,	PUNCT
ajlm-609	181	8	carle	carle	PROPN
ajlm-609	181	9	i	i	PRON
ajlm-609	181	10	,	,	PUNCT
ajlm-609	181	11	et	et	PROPN
ajlm-609	181	12	al	al	PROPN
ajlm-609	181	13	.	.	PUNCT
ajlm-609	181	14	evaluation	evaluation	NOUN
ajlm-609	181	15	of	of	ADP
ajlm-609	181	16	chromagarstec	chromagarstec	NOUN
ajlm-609	181	17	and	and	CCONJ
ajlm-609	181	18	stec	stec	ADJ
ajlm-609	181	19	o104	o104	X
ajlm-609	181	20	chromogenic	chromogenic	ADJ
ajlm-609	181	21	agar	agar	NOUN
ajlm-609	181	22	media	medium	NOUN
ajlm-609	181	23	for	for	ADP
ajlm-609	181	24	detection	detection	NOUN
ajlm-609	181	25	of	of	ADP
ajlm-609	181	26	shiga	shiga	ADJ
ajlm-609	181	27	toxin	toxin	NOUN
ajlm-609	181	28	-	-	PUNCT
ajlm-609	181	29	producing	produce	VERB
ajlm-609	181	30	escherichia	escherichia	NOUN
ajlm-609	181	31	coli	coli	NOUN
ajlm-609	181	32	in	in	ADP
ajlm-609	181	33	stool	stool	NOUN
ajlm-609	181	34	specimens	specimen	NOUN
ajlm-609	181	35	.	.	PUNCT
ajlm-609	182	1	j	j	PROPN
ajlm-609	182	2	clin	clin	PROPN
ajlm-609	182	3	microbiol	microbiol	PROPN
ajlm-609	182	4	.	.	PUNCT
ajlm-609	183	1	2013;51(3):894–900	2013;51(3):894–900	X
ajlm-609	183	2	.	.	PUNCT
ajlm-609	184	1	https://doi.org/10.1128/jcm.03121-12	https://doi.org/10.1128/jcm.03121-12	NOUN
ajlm-609	184	2	hirvonen	hirvonen	PROPN
ajlm-609	184	3	jj	jj	PROPN
ajlm-609	184	4	,	,	PUNCT
ajlm-609	184	5	siitonen	siitonen	NOUN
ajlm-609	184	6	a	a	PRON
ajlm-609	184	7	,	,	PUNCT
ajlm-609	185	1	kaukoranta	kaukoranta	PROPN
ajlm-609	185	2	ss	ss	PROPN
ajlm-609	185	3	.	.	PUNCT
ajlm-609	185	4	usability	usability	NOUN
ajlm-609	185	5	and	and	CCONJ
ajlm-609	185	6	performance	performance	NOUN
ajlm-609	185	7	of	of	ADP
ajlm-609	185	8	chromagarstec	chromagarstec	ADJ
ajlm-609	185	9	medium	medium	NOUN
ajlm-609	185	10	in	in	ADP
ajlm-609	185	11	detection	detection	NOUN
ajlm-609	185	12	of	of	ADP
ajlm-609	185	13	shiga	shiga	ADJ
ajlm-609	185	14	toxin	toxin	NOUN
ajlm-609	185	15	-	-	PUNCT
ajlm-609	185	16	producing	produce	VERB
ajlm-609	185	17	escherichia	escherichia	NOUN
ajlm-609	185	18	coli	coli	NOUN
ajlm-609	185	19	strains	strain	NOUN
ajlm-609	185	20	.	.	PUNCT
ajlm-609	186	1	j	j	PROPN
ajlm-609	186	2	clin	clin	PROPN
ajlm-609	186	3	microbiol	microbiol	PROPN
ajlm-609	186	4	.	.	PUNCT
ajlm-609	187	1	2012;50(11):3586–3590	2012;50(11):3586–3590	NUM
ajlm-609	187	2	.	.	PUNCT
ajlm-609	188	1	https://doi.org/10.1128/jcm.01754-12	https://doi.org/10.1128/jcm.01754-12	PROPN
ajlm-609	188	2	johnson	johnson	PROPN
ajlm-609	188	3	ke	ke	PROPN
ajlm-609	188	4	,	,	PUNCT
ajlm-609	188	5	thorpe	thorpe	PROPN
ajlm-609	188	6	cm	cm	PROPN
ajlm-609	188	7	,	,	PUNCT
ajlm-609	188	8	sears	sears	PROPN
ajlm-609	188	9	cl	cl	PROPN
ajlm-609	188	10	.	.	PUNCT
ajlm-609	189	1	the	the	DET
ajlm-609	189	2	emerging	emerge	VERB
ajlm-609	189	3	clinical	clinical	ADJ
ajlm-609	189	4	importance	importance	NOUN
ajlm-609	189	5	of	of	ADP
ajlm-609	189	6	non	non	ADJ
ajlm-609	189	7	-	-	ADJ
ajlm-609	189	8	o157	o157	ADJ
ajlm-609	189	9	shiga	shiga	ADJ
ajlm-609	189	10	toxin	toxin	NOUN
ajlm-609	189	11	-	-	PUNCT
ajlm-609	189	12	producing	produce	VERB
ajlm-609	189	13	escherichia	escherichia	NOUN
ajlm-609	189	14	coli	coli	NOUN
ajlm-609	189	15	.	.	PUNCT
ajlm-609	190	1	clin	clin	PROPN
ajlm-609	190	2	infect	infect	PROPN
ajlm-609	190	3	dis	dis	PROPN
ajlm-609	190	4	:	:	PUNCT
ajlm-609	190	5	an	an	DET
ajlm-609	190	6	official	official	ADJ
ajlm-609	190	7	publication	publication	NOUN
ajlm-609	190	8	of	of	ADP
ajlm-609	190	9	the	the	DET
ajlm-609	190	10	infectious	infectious	ADJ
ajlm-609	190	11	diseases	disease	NOUN
ajlm-609	190	12	society	society	NOUN
ajlm-609	190	13	of	of	ADP
ajlm-609	190	14	america	america	PROPN
ajlm-609	190	15	.	.	PUNCT
ajlm-609	191	1	2006;43(12):1587–1595	2006;43(12):1587–1595	NOUN
ajlm-609	191	2	.	.	PUNCT
ajlm-609	192	1	https://doi.org/10.1086/509573	https://doi.org/10.1086/509573	VERB
ajlm-609	192	2	karch	karch	PROPN
ajlm-609	192	3	h	h	PROPN
ajlm-609	192	4	,	,	PUNCT
ajlm-609	192	5	bielaszewska	bielaszewska	PROPN
ajlm-609	192	6	m	m	PROPN
ajlm-609	192	7	,	,	PUNCT
ajlm-609	192	8	bitzan	bitzan	NOUN
ajlm-609	192	9	m	m	PROPN
ajlm-609	192	10	,	,	PUNCT
ajlm-609	192	11	et	et	PROPN
ajlm-609	192	12	al	al	PROPN
ajlm-609	192	13	.	.	PUNCT
ajlm-609	193	1	epidemiology	epidemiology	NOUN
ajlm-609	193	2	and	and	CCONJ
ajlm-609	193	3	diagnosis	diagnosis	NOUN
ajlm-609	193	4	of	of	ADP
ajlm-609	193	5	shiga	shiga	ADJ
ajlm-609	193	6	toxin	toxin	NOUN
ajlm-609	193	7	-	-	PUNCT
ajlm-609	193	8	producing	produce	VERB
ajlm-609	193	9	escherichia	escherichia	NOUN
ajlm-609	193	10	coli	coli	NOUN
ajlm-609	193	11	infections	infection	NOUN
ajlm-609	193	12	.	.	PUNCT
ajlm-609	194	1	diagn	diagn	PROPN
ajlm-609	194	2	microbiol	microbiol	PROPN
ajlm-609	194	3	infect	infect	VERB
ajlm-609	194	4	dis	dis	PROPN
ajlm-609	194	5	.	.	PUNCT
ajlm-609	195	1	1999;34(3):229–243	1999;34(3):229–243	NUM
ajlm-609	195	2	.	.	PUNCT
ajlm-609	196	1	https://doi.org/10.1016/s0732-8893(99)00031	https://doi.org/10.1016/s0732-8893(99)00031	PROPN
ajlm-609	196	2	grys	grys	PROPN
ajlm-609	196	3	te	te	PROPN
ajlm-609	196	4	,	,	PUNCT
ajlm-609	196	5	sloan	sloan	PROPN
ajlm-609	196	6	lm	lm	PROPN
ajlm-609	196	7	,	,	PUNCT
ajlm-609	196	8	rosenblatt	rosenblatt	PROPN
ajlm-609	196	9	je	je	PROPN
ajlm-609	196	10	,	,	PUNCT
ajlm-609	196	11	et	et	PROPN
ajlm-609	196	12	al	al	PROPN
ajlm-609	196	13	.	.	PROPN
ajlm-609	197	1	rapid	rapid	ADJ
ajlm-609	197	2	and	and	CCONJ
ajlm-609	197	3	sensitive	sensitive	ADJ
ajlm-609	197	4	detection	detection	NOUN
ajlm-609	197	5	of	of	ADP
ajlm-609	197	6	shiga	shiga	ADJ
ajlm-609	197	7	toxin	toxin	NOUN
ajlm-609	197	8	-	-	PUNCT
ajlm-609	197	9	producing	produce	VERB
ajlm-609	197	10	escherichia	escherichia	NOUN
ajlm-609	197	11	coli	coli	NOUN
ajlm-609	197	12	from	from	ADP
ajlm-609	197	13	nonenriched	nonenriche	VERB
ajlm-609	197	14	stool	stool	NOUN
ajlm-609	197	15	specimens	specimen	NOUN
ajlm-609	197	16	by	by	ADP
ajlm-609	197	17	real	real	ADJ
ajlm-609	197	18	-	-	PUNCT
ajlm-609	197	19	time	time	NOUN
ajlm-609	197	20	pcr	pcr	NOUN
ajlm-609	197	21	in	in	ADP
ajlm-609	197	22	comparison	comparison	NOUN
ajlm-609	197	23	to	to	ADP
ajlm-609	197	24	enzyme	enzyme	VERB
ajlm-609	197	25	immunoassay	immunoassay	NOUN
ajlm-609	197	26	and	and	CCONJ
ajlm-609	197	27	culture	culture	NOUN
ajlm-609	197	28	.	.	PUNCT
ajlm-609	198	1	j	j	PROPN
ajlm-609	198	2	clin	clin	PROPN
ajlm-609	198	3	microbiol	microbiol	PROPN
ajlm-609	198	4	.	.	PUNCT
ajlm-609	199	1	2009;47(7):2008–2012	2009;47(7):2008–2012	NUM
ajlm-609	199	2	.	.	PUNCT
ajlm-609	200	1	https://doi.org/10.1128/jcm.02013-08	https://doi.org/10.1128/jcm.02013-08	NOUN
ajlm-609	200	2	perna	perna	PROPN
ajlm-609	200	3	nt	nt	PROPN
ajlm-609	200	4	,	,	PUNCT
ajlm-609	200	5	plunkett	plunkett	PROPN
ajlm-609	200	6	g	g	PROPN
ajlm-609	200	7	,	,	PUNCT
ajlm-609	200	8	3rd	3rd	NOUN
ajlm-609	200	9	,	,	PUNCT
ajlm-609	200	10	burland	burland	NOUN
ajlm-609	200	11	v	v	NOUN
ajlm-609	200	12	,	,	PUNCT
ajlm-609	200	13	et	et	PROPN
ajlm-609	200	14	al	al	PROPN
ajlm-609	200	15	.	.	PROPN
ajlm-609	200	16	genome	genome	NOUN
ajlm-609	200	17	sequence	sequence	NOUN
ajlm-609	200	18	of	of	ADP
ajlm-609	200	19	enterohaemorrhagic	enterohaemorrhagic	NOUN
ajlm-609	200	20	escherichia	escherichia	NOUN
ajlm-609	200	21	coli	coli	NOUN
ajlm-609	200	22	o157	o157	PROPN
ajlm-609	200	23	:	:	PUNCT
ajlm-609	200	24	h7	h7	PROPN
ajlm-609	200	25	.	.	PUNCT
ajlm-609	200	26	nature	nature	PROPN
ajlm-609	200	27	.	.	PUNCT
ajlm-609	201	1	2001;409(6819):529–533	2001;409(6819):529–533	X
ajlm-609	201	2	.	.	PUNCT
ajlm-609	202	1	https://doi.org/10.1038/35054089	https://doi.org/10.1038/35054089	NOUN
ajlm-609	202	2	paton	paton	PROPN
ajlm-609	202	3	aw	aw	INTJ
ajlm-609	202	4	,	,	PUNCT
ajlm-609	202	5	paton	paton	PROPN
ajlm-609	202	6	jc	jc	PROPN
ajlm-609	202	7	.	.	PROPN
ajlm-609	203	1	detection	detection	NOUN
ajlm-609	203	2	and	and	CCONJ
ajlm-609	203	3	characterization	characterization	NOUN
ajlm-609	203	4	of	of	ADP
ajlm-609	203	5	shigatoxigenic	shigatoxigenic	ADJ
ajlm-609	203	6	escherichia	escherichia	NOUN
ajlm-609	203	7	coli	coli	NOUN
ajlm-609	203	8	by	by	ADP
ajlm-609	203	9	using	use	VERB
ajlm-609	203	10	multiplex	multiplex	PROPN
ajlm-609	203	11	pcr	pcr	ADJ
ajlm-609	203	12	assays	assay	NOUN
ajlm-609	203	13	for	for	ADP
ajlm-609	203	14	stx1	stx1	NOUN
ajlm-609	203	15	,	,	PUNCT
ajlm-609	203	16	stx2	stx2	PROPN
ajlm-609	203	17	,	,	PUNCT
ajlm-609	203	18	eaea	eaea	VERB
ajlm-609	203	19	,	,	PUNCT
ajlm-609	203	20	enterohemorrhagic	enterohemorrhagic	PROPN
ajlm-609	203	21	e.	e.	PROPN
ajlm-609	203	22	coli	coli	PROPN
ajlm-609	203	23	hlya	hlya	PROPN
ajlm-609	203	24	,	,	PUNCT
ajlm-609	203	25	rfbo111	rfbo111	PROPN
ajlm-609	203	26	,	,	PUNCT
ajlm-609	203	27	and	and	CCONJ
ajlm-609	203	28	rfbo157	rfbo157	PROPN
ajlm-609	203	29	.	.	PUNCT
ajlm-609	204	1	j	j	PROPN
ajlm-609	204	2	clin	clin	PROPN
ajlm-609	204	3	microbiol	microbiol	PROPN
ajlm-609	204	4	.	.	PUNCT
ajlm-609	205	1	1998;36(2):598–602	1998;36(2):598–602	X
ajlm-609	205	2	.	.	PUNCT
ajlm-609	206	1	bettelheim	bettelheim	PROPN
ajlm-609	206	2	ka	ka	PROPN
ajlm-609	206	3	,	,	PUNCT
ajlm-609	206	4	thompson	thompson	PROPN
ajlm-609	206	5	cj	cj	PROPN
ajlm-609	206	6	.	.	PUNCT
ajlm-609	207	1	new	new	ADJ
ajlm-609	207	2	method	method	NOUN
ajlm-609	207	3	of	of	ADP
ajlm-609	207	4	serotyping	serotyping	NOUN
ajlm-609	207	5	escherichia	escherichia	NOUN
ajlm-609	207	6	coli	coli	NOUN
ajlm-609	207	7	:	:	PUNCT
ajlm-609	207	8	implementation	implementation	NOUN
ajlm-609	207	9	and	and	CCONJ
ajlm-609	207	10	verification	verification	NOUN
ajlm-609	207	11	.	.	PUNCT
ajlm-609	208	1	j	j	PROPN
ajlm-609	208	2	clin	clin	PROPN
ajlm-609	208	3	microbiol	microbiol	PROPN
ajlm-609	208	4	.	.	PUNCT
ajlm-609	209	1	1987;25(5):781–786	1987;25(5):781–786	NUM
ajlm-609	209	2	.	.	PUNCT
ajlm-609	210	1	tamura	tamura	PROPN
ajlm-609	210	2	k	k	PROPN
ajlm-609	210	3	,	,	PUNCT
ajlm-609	210	4	sakazaki	sakazaki	ADJ
ajlm-609	210	5	r	r	NOUN
ajlm-609	210	6	,	,	PUNCT
ajlm-609	210	7	murase	murase	NOUN
ajlm-609	210	8	m	m	PROPN
ajlm-609	210	9	,	,	PUNCT
ajlm-609	210	10	kosako	kosako	PROPN
ajlm-609	210	11	y.	y.	PROPN
ajlm-609	210	12	serotyping	serotyping	NOUN
ajlm-609	210	13	and	and	CCONJ
ajlm-609	210	14	categorisation	categorisation	NOUN
ajlm-609	210	15	of	of	ADP
ajlm-609	210	16	escherichia	escherichia	NOUN
ajlm-609	210	17	coli	coli	NOUN
ajlm-609	210	18	strains	strain	NOUN
ajlm-609	210	19	isolated	isolate	VERB
ajlm-609	210	20	between	between	ADP
ajlm-609	210	21	1958	1958	NUM
ajlm-609	210	22	and	and	CCONJ
ajlm-609	210	23	1992	1992	NUM
ajlm-609	210	24	from	from	ADP
ajlm-609	210	25	diarrhoeal	diarrhoeal	ADJ
ajlm-609	210	26	diseases	disease	NOUN
ajlm-609	210	27	in	in	ADP
ajlm-609	210	28	asia	asia	PROPN
ajlm-609	210	29	.	.	PUNCT
ajlm-609	211	1	j	j	PROPN
ajlm-609	211	2	med	med	PROPN
ajlm-609	211	3	microbiol	microbiol	PROPN
ajlm-609	211	4	.	.	PUNCT
ajlm-609	212	1	1996;45(5):353–358	1996;45(5):353–358	X
ajlm-609	212	2	.	.	PUNCT
ajlm-609	212	3	https://doi.org/10.1099/00222615-45-5-353	https://doi.org/10.1099/00222615-45-5-353	NUM
ajlm-609	212	4	olesen	olesen	ADP
ajlm-609	212	5	b	b	PROPN
ajlm-609	212	6	,	,	PUNCT
ajlm-609	212	7	jensen	jensen	PROPN
ajlm-609	212	8	c	c	PROPN
ajlm-609	212	9	,	,	PUNCT
ajlm-609	212	10	olsen	olsen	PROPN
ajlm-609	212	11	k	k	PROPN
ajlm-609	212	12	,	,	PUNCT
ajlm-609	212	13	et	et	PROPN
ajlm-609	212	14	al	al	PROPN
ajlm-609	212	15	.	.	PUNCT
ajlm-609	213	1	vtec	vtec	PROPN
ajlm-609	213	2	o117	o117	NUM
ajlm-609	213	3	:	:	PUNCT
ajlm-609	213	4	k1	k1	NOUN
ajlm-609	213	5	:	:	PUNCT
ajlm-609	213	6	h7	h7	PROPN
ajlm-609	213	7	.	.	PUNCT
ajlm-609	214	1	a	a	DET
ajlm-609	214	2	new	new	ADJ
ajlm-609	214	3	clonal	clonal	ADJ
ajlm-609	214	4	group	group	NOUN
ajlm-609	214	5	of	of	ADP
ajlm-609	214	6	e.	e.	PROPN
ajlm-609	214	7	coli	coli	PROPN
ajlm-609	214	8	associated	associate	VERB
ajlm-609	214	9	with	with	ADP
ajlm-609	214	10	persistent	persistent	ADJ
ajlm-609	214	11	diarrhoea	diarrhoea	NOUN
ajlm-609	214	12	in	in	ADP
ajlm-609	214	13	danish	danish	ADJ
ajlm-609	214	14	travellers	traveller	NOUN
ajlm-609	214	15	.	.	PUNCT
ajlm-609	215	1	scand	scand	PROPN
ajlm-609	215	2	j	j	PROPN
ajlm-609	215	3	infect	infect	VERB
ajlm-609	215	4	dis	dis	PROPN
ajlm-609	215	5	.	.	PUNCT
ajlm-609	216	1	2005;37(4):288–294	2005;37(4):288–294	NUM
ajlm-609	216	2	.	.	PUNCT
ajlm-609	217	1	https://doi.org/10.1080/00365540410021090	https://doi.org/10.1080/00365540410021090	DET
ajlm-609	217	2	wylie	wylie	PROPN
ajlm-609	217	3	jl	jl	PROPN
ajlm-609	217	4	,	,	PUNCT
ajlm-609	217	5	van	van	PROPN
ajlm-609	217	6	caeseele	caeseele	PROPN
ajlm-609	217	7	p	p	X
ajlm-609	217	8	,	,	PUNCT
ajlm-609	217	9	gilmour	gilmour	PROPN
ajlm-609	217	10	mw	mw	PROPN
ajlm-609	217	11	,	,	PUNCT
ajlm-609	217	12	et	et	PROPN
ajlm-609	217	13	al	al	PROPN
ajlm-609	217	14	.	.	PUNCT
ajlm-609	217	15	evaluation	evaluation	NOUN
ajlm-609	217	16	of	of	ADP
ajlm-609	217	17	a	a	DET
ajlm-609	217	18	new	new	ADJ
ajlm-609	217	19	chromogenic	chromogenic	ADJ
ajlm-609	217	20	agar	agar	NOUN
ajlm-609	217	21	medium	medium	NOUN
ajlm-609	217	22	for	for	ADP
ajlm-609	217	23	detection	detection	NOUN
ajlm-609	217	24	of	of	ADP
ajlm-609	217	25	shiga	shiga	ADJ
ajlm-609	217	26	toxin	toxin	NOUN
ajlm-609	217	27	-	-	PUNCT
ajlm-609	217	28	producing	produce	VERB
ajlm-609	217	29	escherichia	escherichia	NOUN
ajlm-609	217	30	coli	coli	NOUN
ajlm-609	217	31	(	(	PUNCT
ajlm-609	217	32	stec	stec	ADJ
ajlm-609	217	33	)	)	PUNCT
ajlm-609	217	34	and	and	CCONJ
ajlm-609	217	35	relative	relative	ADJ
ajlm-609	217	36	prevalences	prevalence	NOUN
ajlm-609	217	37	of	of	ADP
ajlm-609	217	38	o157	o157	PROPN
ajlm-609	217	39	and	and	CCONJ
ajlm-609	217	40	non	non	ADJ
ajlm-609	217	41	-	-	ADJ
ajlm-609	217	42	o157	o157	ADJ
ajlm-609	217	43	stec	stec	ADJ
ajlm-609	217	44	in	in	ADP
ajlm-609	217	45	manitoba	manitoba	PROPN
ajlm-609	217	46	,	,	PUNCT
ajlm-609	217	47	canada	canada	PROPN
ajlm-609	217	48	.	.	PUNCT
ajlm-609	218	1	j	j	PROPN
ajlm-609	218	2	clin	clin	PROPN
ajlm-609	218	3	microbiol	microbiol	PROPN
ajlm-609	218	4	.	.	PUNCT
ajlm-609	219	1	2013;51(2):466–471	2013;51(2):466–471	NUM
ajlm-609	219	2	.	.	PUNCT
ajlm-609	220	1	https://doi.org/10.1128/jcm.02329-12	https://doi.org/10.1128/jcm.02329-12	NOUN
ajlm-609	220	2	tzschoppe	tzschoppe	PROPN
ajlm-609	220	3	m	m	PROPN
ajlm-609	220	4	,	,	PUNCT
ajlm-609	220	5	martin	martin	PROPN
ajlm-609	220	6	a	a	PRON
ajlm-609	220	7	,	,	PUNCT
ajlm-609	220	8	beutin	beutin	PROPN
ajlm-609	220	9	l.	l.	PROPN
ajlm-609	220	10	a	a	DET
ajlm-609	220	11	rapid	rapid	ADJ
ajlm-609	220	12	procedure	procedure	NOUN
ajlm-609	220	13	for	for	ADP
ajlm-609	220	14	the	the	DET
ajlm-609	220	15	detection	detection	NOUN
ajlm-609	220	16	and	and	CCONJ
ajlm-609	220	17	isolation	isolation	NOUN
ajlm-609	220	18	of	of	ADP
ajlm-609	220	19	enterohaemorrhagic	enterohaemorrhagic	NOUN
ajlm-609	220	20	escherichia	escherichia	NOUN
ajlm-609	220	21	coli	coli	NOUN
ajlm-609	220	22	(	(	PUNCT
ajlm-609	220	23	ehec	ehec	PROPN
ajlm-609	220	24	)	)	PUNCT
ajlm-609	220	25	serogroup	serogroup	NOUN
ajlm-609	220	26	o26	o26	VERB
ajlm-609	220	27	,	,	PUNCT
ajlm-609	220	28	o103	o103	NUM
ajlm-609	220	29	,	,	PUNCT
ajlm-609	220	30	o111	o111	PROPN
ajlm-609	220	31	,	,	PUNCT
ajlm-609	220	32	o118	o118	NUM
ajlm-609	220	33	,	,	PUNCT
ajlm-609	220	34	o121	o121	NUM
ajlm-609	220	35	,	,	PUNCT
ajlm-609	220	36	o145	o145	PROPN
ajlm-609	220	37	and	and	CCONJ
ajlm-609	220	38	o157	o157	PROPN
ajlm-609	220	39	strains	strain	NOUN
ajlm-609	220	40	and	and	CCONJ
ajlm-609	220	41	the	the	DET
ajlm-609	220	42	aggregative	aggregative	ADJ
ajlm-609	220	43	ehec	ehec	PROPN
ajlm-609	220	44	o104	o104	PROPN
ajlm-609	220	45	:	:	PUNCT
ajlm-609	220	46	h4	h4	PROPN
ajlm-609	220	47	strain	strain	VERB
ajlm-609	220	48	from	from	ADP
ajlm-609	220	49	ready	ready	ADJ
ajlm-609	220	50	-	-	PUNCT
ajlm-609	220	51	to	to	PART
ajlm-609	220	52	-	-	PUNCT
ajlm-609	220	53	eat	eat	VERB
ajlm-609	220	54	vegetables	vegetable	NOUN
ajlm-609	220	55	.	.	PUNCT
ajlm-609	221	1	int	int	NOUN
ajlm-609	221	2	j	j	PROPN
ajlm-609	221	3	food	food	PROPN
ajlm-609	221	4	microbiol	microbiol	PROPN
ajlm-609	221	5	.	.	PUNCT
ajlm-609	222	1	2012;152(1–2):19–30	2012;152(1–2):19–30	PROPN
ajlm-609	222	2	.	.	PUNCT
ajlm-609	222	3	https://doi.org/10.1016/j.ijfoodmicro.2011.10.009	https://doi.org/10.1016/j.ijfoodmicro.2011.10.009	PROPN
ajlm-609	222	4	kullin	kullin	PROPN
ajlm-609	222	5	b	b	PROPN
ajlm-609	222	6	,	,	PUNCT
ajlm-609	222	7	meggersee	meggersee	ADJ
ajlm-609	222	8	r	r	PROPN
ajlm-609	222	9	,	,	PUNCT
ajlm-609	222	10	d’alton	d’alton	PROPN
ajlm-609	222	11	j	j	PROPN
ajlm-609	222	12	,	,	PUNCT
ajlm-609	222	13	et	et	PROPN
ajlm-609	222	14	al	al	PROPN
ajlm-609	222	15	.	.	PUNCT
ajlm-609	223	1	prevalence	prevalence	NOUN
ajlm-609	223	2	of	of	ADP
ajlm-609	223	3	gastrointestinal	gastrointestinal	ADJ
ajlm-609	223	4	pathogenic	pathogenic	ADJ
ajlm-609	223	5	bacteria	bacteria	NOUN
ajlm-609	223	6	in	in	ADP
ajlm-609	223	7	patients	patient	NOUN
ajlm-609	223	8	with	with	ADP
ajlm-609	223	9	diarrhoea	diarrhoea	NOUN
ajlm-609	223	10	attending	attend	VERB
ajlm-609	223	11	groote	groote	ADJ
ajlm-609	223	12	schuur	schuur	PROPN
ajlm-609	223	13	hospital	hospital	PROPN
ajlm-609	223	14	,	,	PUNCT
ajlm-609	223	15	cape	cape	NOUN
ajlm-609	223	16	town	town	NOUN
ajlm-609	223	17	,	,	PUNCT
ajlm-609	223	18	south	south	PROPN
ajlm-609	223	19	africa	africa	PROPN
ajlm-609	223	20	.	.	PUNCT
ajlm-609	224	1	samj	samj	PROPN
ajlm-609	224	2	.	.	PUNCT
ajlm-609	225	1	2015;105:121–125	2015;105:121–125	NUM
ajlm-609	225	2	.	.	PUNCT
ajlm-609	225	3	https://doi.org/10.7196/samj.8654	https://doi.org/10.7196/samj.8654	PROPN
ajlm-609	225	4	hegde	hegde	PROPN
ajlm-609	225	5	nv	nv	PROPN
ajlm-609	225	6	,	,	PUNCT
ajlm-609	225	7	cote	cote	PROPN
ajlm-609	225	8	r	r	PROPN
ajlm-609	225	9	,	,	PUNCT
ajlm-609	225	10	jayarao	jayarao	PROPN
ajlm-609	225	11	bm	bm	PROPN
ajlm-609	225	12	,	,	PUNCT
ajlm-609	225	13	et	et	PROPN
ajlm-609	225	14	al	al	PROPN
ajlm-609	225	15	.	.	PUNCT
ajlm-609	225	16	detection	detection	NOUN
ajlm-609	225	17	of	of	ADP
ajlm-609	225	18	the	the	DET
ajlm-609	225	19	top	top	ADJ
ajlm-609	225	20	six	six	NUM
ajlm-609	225	21	non	non	ADJ
ajlm-609	225	22	-	-	ADJ
ajlm-609	225	23	o157	o157	ADJ
ajlm-609	225	24	shiga	shiga	ADJ
ajlm-609	225	25	toxin	toxin	NOUN
ajlm-609	225	26	-	-	PUNCT
ajlm-609	225	27	producing	produce	VERB
ajlm-609	225	28	escherichia	escherichia	NOUN
ajlm-609	225	29	coli	coli	NOUN
ajlm-609	225	30	o	o	NOUN
ajlm-609	225	31	groups	group	NOUN
ajlm-609	225	32	by	by	ADP
ajlm-609	225	33	elisa	elisa	PROPN
ajlm-609	225	34	.	.	PUNCT
ajlm-609	226	1	foodborne	foodborne	PROPN
ajlm-609	226	2	pathog	pathog	PROPN
ajlm-609	226	3	dis	dis	PROPN
ajlm-609	226	4	.	.	PUNCT
ajlm-609	227	1	2012;9(11):1044–1048	2012;9(11):1044–1048	NOUN
ajlm-609	227	2	.	.	PUNCT
ajlm-609	228	1	https://doi.org/10.1089/fpd.2012.1231	https://doi.org/10.1089/fpd.2012.1231	PROPN
ajlm-609	228	2	buchholz	buchholz	PROPN
ajlm-609	228	3	u	u	PROPN
ajlm-609	228	4	,	,	PUNCT
ajlm-609	228	5	bernard	bernard	PROPN
ajlm-609	228	6	h	h	PROPN
ajlm-609	228	7	,	,	PUNCT
ajlm-609	228	8	werber	werber	PROPN
ajlm-609	228	9	d	d	PROPN
ajlm-609	228	10	,	,	PUNCT
ajlm-609	228	11	et	et	PROPN
ajlm-609	228	12	al	al	PROPN
ajlm-609	228	13	.	.	PUNCT
ajlm-609	229	1	german	german	ADJ
ajlm-609	229	2	outbreak	outbreak	NOUN
ajlm-609	229	3	of	of	ADP
ajlm-609	229	4	escherichia	escherichia	NOUN
ajlm-609	229	5	coli	coli	NOUN
ajlm-609	229	6	o104	o104	PROPN
ajlm-609	229	7	:	:	PUNCT
ajlm-609	229	8	h4	h4	NOUN
ajlm-609	229	9	associated	associate	VERB
ajlm-609	229	10	with	with	ADP
ajlm-609	229	11	sprouts	sprout	NOUN
ajlm-609	229	12	.	.	PUNCT
ajlm-609	230	1	n	n	PROPN
ajlm-609	230	2	engl	engl	PROPN
ajlm-609	230	3	j	j	PROPN
ajlm-609	230	4	med	med	PROPN
ajlm-609	230	5	.	.	PUNCT
ajlm-609	231	1	2011;365(19):1763–1770	2011;365(19):1763–1770	NUM
ajlm-609	231	2	.	.	PUNCT
ajlm-609	232	1	https://doi.org/10.1016/j.yped.2011.12.017	https://doi.org/10.1016/j.yped.2011.12.017	ADP
ajlm-609	232	2	bielaszewska	bielaszewska	NOUN
ajlm-609	232	3	m	m	NOUN
ajlm-609	232	4	,	,	PUNCT
ajlm-609	232	5	kock	kock	PROPN
ajlm-609	232	6	r	r	PROPN
ajlm-609	232	7	,	,	PUNCT
ajlm-609	232	8	friedrich	friedrich	NOUN
ajlm-609	232	9	aw	aw	INTJ
ajlm-609	232	10	,	,	PUNCT
ajlm-609	232	11	et	et	PROPN
ajlm-609	232	12	al	al	PROPN
ajlm-609	232	13	.	.	PUNCT
ajlm-609	232	14	shiga	shiga	ADJ
ajlm-609	232	15	toxin	toxin	NOUN
ajlm-609	232	16	-	-	PUNCT
ajlm-609	232	17	mediated	mediate	VERB
ajlm-609	232	18	hemolytic	hemolytic	ADJ
ajlm-609	232	19	uremic	uremic	ADJ
ajlm-609	232	20	syndrome	syndrome	NOUN
ajlm-609	232	21	:	:	PUNCT
ajlm-609	232	22	time	time	NOUN
ajlm-609	232	23	to	to	PART
ajlm-609	232	24	change	change	VERB
ajlm-609	232	25	the	the	DET
ajlm-609	232	26	diagnostic	diagnostic	ADJ
ajlm-609	232	27	paradigm	paradigm	NOUN
ajlm-609	232	28	?	?	PUNCT
ajlm-609	233	1	plos	plos	PROPN
ajlm-609	233	2	one	one	NUM
ajlm-609	233	3	.	.	PUNCT
ajlm-609	234	1	2007;2(10):e1024	2007;2(10):e1024	NUM
ajlm-609	234	2	.	.	PUNCT
ajlm-609	235	1	https://doi.org/10.1371/journal.pone.0001024	https://doi.org/10.1371/journal.pone.0001024	NOUN
ajlm-609	235	2	jenkins	jenkin	NOUN
ajlm-609	235	3	c	c	PROPN
ajlm-609	235	4	,	,	PUNCT
ajlm-609	235	5	lawson	lawson	PROPN
ajlm-609	235	6	aj	aj	PROPN
ajlm-609	235	7	,	,	PUNCT
ajlm-609	235	8	cheasty	cheasty	PROPN
ajlm-609	235	9	t	t	PROPN
ajlm-609	235	10	,	,	PUNCT
ajlm-609	235	11	willshaw	willshaw	PROPN
ajlm-609	235	12	ga	ga	PROPN
ajlm-609	235	13	.	.	PROPN
ajlm-609	235	14	assessment	assessment	NOUN
ajlm-609	235	15	of	of	ADP
ajlm-609	235	16	a	a	DET
ajlm-609	235	17	real	real	ADJ
ajlm-609	235	18	-	-	PUNCT
ajlm-609	235	19	time	time	NOUN
ajlm-609	235	20	pcr	pcr	NOUN
ajlm-609	235	21	for	for	ADP
ajlm-609	235	22	the	the	DET
ajlm-609	235	23	detection	detection	NOUN
ajlm-609	235	24	and	and	CCONJ
ajlm-609	235	25	characterization	characterization	NOUN
ajlm-609	235	26	of	of	ADP
ajlm-609	235	27	verocytotoxigenic	verocytotoxigenic	ADJ
ajlm-609	235	28	escherichia	escherichia	NOUN
ajlm-609	235	29	coli	coli	NOUN
ajlm-609	235	30	.	.	PUNCT
ajlm-609	236	1	j	j	PROPN
ajlm-609	236	2	med	med	PROPN
ajlm-609	236	3	microbiol	microbiol	PROPN
ajlm-609	236	4	.	.	PUNCT
ajlm-609	237	1	2012;61(pt	2012;61(pt	ADJ
ajlm-609	237	2	8):1082–1085	8):1082–1085	NOUN
ajlm-609	237	3	.	.	PUNCT
ajlm-609	238	1	https://doi.org/10.1099/jmm.0.041517-0	https://doi.org/10.1099/jmm.0.041517-0	PROPN