id	sid	tid	token	lemma	pos
ajlm-658	1	1	abstract	abstract	ADJ
ajlm-658	1	2	introduction	introduction	NOUN
ajlm-658	1	3	methods	method	NOUN
ajlm-658	1	4	results	result	VERB
ajlm-658	1	5	discussion	discussion	NOUN
ajlm-658	1	6	acknowledgements	acknowledgement	NOUN
ajlm-658	1	7	references	reference	NOUN
ajlm-658	1	8	about	about	ADP
ajlm-658	1	9	the	the	DET
ajlm-658	1	10	author(s	author(s	NOUN
ajlm-658	1	11	)	)	PUNCT
ajlm-658	1	12	tshiphiri	tshiphiri	NOUN
ajlm-658	1	13	senamela	senamela	PROPN
ajlm-658	1	14	national	national	PROPN
ajlm-658	1	15	health	health	PROPN
ajlm-658	1	16	laboratory	laboratory	PROPN
ajlm-658	1	17	services	services	PROPN
ajlm-658	1	18	,	,	PUNCT
ajlm-658	1	19	pretoria	pretoria	PROPN
ajlm-658	1	20	,	,	PUNCT
ajlm-658	1	21	south	south	PROPN
ajlm-658	1	22	africa	africa	PROPN
ajlm-658	1	23	department	department	PROPN
ajlm-658	1	24	of	of	ADP
ajlm-658	1	25	haematology	haematology	PROPN
ajlm-658	1	26	,	,	PUNCT
ajlm-658	1	27	faculty	faculty	NOUN
ajlm-658	1	28	of	of	ADP
ajlm-658	1	29	health	health	NOUN
ajlm-658	1	30	sciences	sciences	PROPN
ajlm-658	1	31	,	,	PUNCT
ajlm-658	1	32	university	university	NOUN
ajlm-658	1	33	of	of	ADP
ajlm-658	1	34	pretoria	pretoria	PROPN
ajlm-658	1	35	,	,	PUNCT
ajlm-658	1	36	pretoria	pretoria	PROPN
ajlm-658	1	37	,	,	PUNCT
ajlm-658	1	38	south	south	PROPN
ajlm-658	1	39	africa	africa	PROPN
ajlm-658	1	40	marleen	marleen	PROPN
ajlm-658	1	41	kock	kock	PROPN
ajlm-658	1	42	national	national	PROPN
ajlm-658	1	43	health	health	PROPN
ajlm-658	1	44	laboratory	laboratory	PROPN
ajlm-658	1	45	services	services	PROPN
ajlm-658	1	46	,	,	PUNCT
ajlm-658	1	47	pretoria	pretoria	PROPN
ajlm-658	1	48	,	,	PUNCT
ajlm-658	1	49	south	south	PROPN
ajlm-658	1	50	africa	africa	PROPN
ajlm-658	1	51	department	department	PROPN
ajlm-658	1	52	of	of	ADP
ajlm-658	1	53	haematology	haematology	PROPN
ajlm-658	1	54	,	,	PUNCT
ajlm-658	1	55	faculty	faculty	NOUN
ajlm-658	1	56	of	of	ADP
ajlm-658	1	57	health	health	NOUN
ajlm-658	1	58	sciences	sciences	PROPN
ajlm-658	1	59	,	,	PUNCT
ajlm-658	1	60	university	university	NOUN
ajlm-658	1	61	of	of	ADP
ajlm-658	1	62	pretoria	pretoria	PROPN
ajlm-658	1	63	,	,	PUNCT
ajlm-658	1	64	pretoria	pretoria	PROPN
ajlm-658	1	65	,	,	PUNCT
ajlm-658	1	66	south	south	PROPN
ajlm-658	1	67	africa	africa	PROPN
ajlm-658	1	68	piet	piet	PROPN
ajlm-658	1	69	becker	becker	PROPN
ajlm-658	1	70	department	department	PROPN
ajlm-658	1	71	of	of	ADP
ajlm-658	1	72	haematology	haematology	PROPN
ajlm-658	1	73	,	,	PUNCT
ajlm-658	1	74	faculty	faculty	NOUN
ajlm-658	1	75	of	of	ADP
ajlm-658	1	76	health	health	NOUN
ajlm-658	1	77	sciences	sciences	PROPN
ajlm-658	1	78	,	,	PUNCT
ajlm-658	1	79	university	university	NOUN
ajlm-658	1	80	of	of	ADP
ajlm-658	1	81	pretoria	pretoria	PROPN
ajlm-658	1	82	,	,	PUNCT
ajlm-658	1	83	pretoria	pretoria	PROPN
ajlm-658	1	84	,	,	PUNCT
ajlm-658	1	85	south	south	PROPN
ajlm-658	1	86	africa	africa	PROPN
ajlm-658	1	87	joachim	joachim	PROPN
ajlm-658	1	88	j.c	j.c	PROPN
ajlm-658	1	89	.	.	PROPN
ajlm-658	1	90	potgieter	potgieter	PROPN
ajlm-658	1	91	national	national	PROPN
ajlm-658	1	92	health	health	PROPN
ajlm-658	1	93	laboratory	laboratory	PROPN
ajlm-658	1	94	services	services	PROPN
ajlm-658	1	95	,	,	PUNCT
ajlm-658	1	96	pretoria	pretoria	PROPN
ajlm-658	1	97	,	,	PUNCT
ajlm-658	1	98	south	south	PROPN
ajlm-658	1	99	africa	africa	PROPN
ajlm-658	1	100	department	department	PROPN
ajlm-658	1	101	of	of	ADP
ajlm-658	1	102	haematology	haematology	PROPN
ajlm-658	1	103	,	,	PUNCT
ajlm-658	1	104	faculty	faculty	NOUN
ajlm-658	1	105	of	of	ADP
ajlm-658	1	106	health	health	NOUN
ajlm-658	1	107	sciences	sciences	PROPN
ajlm-658	1	108	,	,	PUNCT
ajlm-658	1	109	university	university	NOUN
ajlm-658	1	110	of	of	ADP
ajlm-658	1	111	pretoria	pretoria	PROPN
ajlm-658	1	112	,	,	PUNCT
ajlm-658	1	113	pretoria	pretoria	PROPN
ajlm-658	1	114	,	,	PUNCT
ajlm-658	1	115	south	south	PROPN
ajlm-658	1	116	africa	africa	PROPN
ajlm-658	1	117	citation	citation	PROPN
ajlm-658	1	118	senamela	senamela	PROPN
ajlm-658	1	119	t	t	PROPN
ajlm-658	1	120	,	,	PUNCT
ajlm-658	1	121	kock	kock	PROPN
ajlm-658	1	122	m	m	PROPN
ajlm-658	1	123	,	,	PUNCT
ajlm-658	1	124	becker	becker	PROPN
ajlm-658	2	1	p	p	X
ajlm-658	2	2	,	,	PUNCT
ajlm-658	2	3	et	et	PROPN
ajlm-658	2	4	al	al	PROPN
ajlm-658	2	5	.	.	PUNCT
ajlm-658	2	6	detection	detection	NOUN
ajlm-658	2	7	of	of	ADP
ajlm-658	2	8	the	the	DET
ajlm-658	2	9	janus	janus	PROPN
ajlm-658	2	10	kinase	kinase	PROPN
ajlm-658	2	11	2	2	NUM
ajlm-658	2	12	v617f	v617f	PROPN
ajlm-658	2	13	mutation	mutation	NOUN
ajlm-658	2	14	using	use	VERB
ajlm-658	2	15	a	a	DET
ajlm-658	2	16	locked	locked	ADJ
ajlm-658	2	17	nucleic	nucleic	ADJ
ajlm-658	2	18	-	-	PUNCT
ajlm-658	2	19	acid	acid	ADJ
ajlm-658	2	20	,	,	PUNCT
ajlm-658	2	21	real	real	ADJ
ajlm-658	2	22	-	-	PUNCT
ajlm-658	2	23	time	time	NOUN
ajlm-658	2	24	polymerase	polymerase	NOUN
ajlm-658	2	25	chain	chain	NOUN
ajlm-658	2	26	reaction	reaction	NOUN
ajlm-658	2	27	assay	assay	VERB
ajlm-658	2	28	.	.	PUNCT
ajlm-658	3	1	afr	afr	PROPN
ajlm-658	3	2	j	j	PROPN
ajlm-658	3	3	lab	lab	PROPN
ajlm-658	3	4	med	med	PROPN
ajlm-658	3	5	.	.	PUNCT
ajlm-658	3	6	2018;7(1	2018;7(1	NUM
ajlm-658	3	7	)	)	PUNCT
ajlm-658	3	8	,	,	PUNCT
ajlm-658	3	9	a658	a658	PROPN
ajlm-658	3	10	.	.	PROPN
ajlm-658	3	11	https://doi.org/10.4102/ajlm.v7i1.658	https://doi.org/10.4102/ajlm.v7i1.658	PROPN
ajlm-658	3	12	brief	brief	PROPN
ajlm-658	3	13	report	report	NOUN
ajlm-658	3	14	detection	detection	NOUN
ajlm-658	3	15	of	of	ADP
ajlm-658	3	16	the	the	DET
ajlm-658	3	17	janus	janus	PROPN
ajlm-658	3	18	kinase	kinase	PROPN
ajlm-658	3	19	2	2	NUM
ajlm-658	3	20	v617f	v617f	PROPN
ajlm-658	3	21	mutation	mutation	NOUN
ajlm-658	3	22	using	use	VERB
ajlm-658	3	23	a	a	DET
ajlm-658	3	24	locked	locked	ADJ
ajlm-658	3	25	nucleic	nucleic	ADJ
ajlm-658	3	26	-	-	PUNCT
ajlm-658	3	27	acid	acid	ADJ
ajlm-658	3	28	,	,	PUNCT
ajlm-658	3	29	real	real	ADJ
ajlm-658	3	30	-	-	PUNCT
ajlm-658	3	31	time	time	NOUN
ajlm-658	3	32	polymerase	polymerase	NOUN
ajlm-658	3	33	chain	chain	NOUN
ajlm-658	3	34	reaction	reaction	NOUN
ajlm-658	3	35	assay	assay	PROPN
ajlm-658	3	36	tshiphiri	tshiphiri	NOUN
ajlm-658	3	37	senamela	senamela	PROPN
ajlm-658	3	38	,	,	PUNCT
ajlm-658	3	39	marleen	marleen	PROPN
ajlm-658	3	40	kock	kock	PROPN
ajlm-658	3	41	,	,	PUNCT
ajlm-658	3	42	piet	piet	PROPN
ajlm-658	3	43	becker	becker	PROPN
ajlm-658	3	44	,	,	PUNCT
ajlm-658	3	45	joachim	joachim	PROPN
ajlm-658	3	46	j.c	j.c	PROPN
ajlm-658	3	47	.	.	PROPN
ajlm-658	3	48	potgieter	potgieter	PROPN
ajlm-658	3	49	received	receive	VERB
ajlm-658	3	50	:	:	PUNCT
ajlm-658	3	51	30	30	NUM
ajlm-658	3	52	jun	jun	PROPN
ajlm-658	3	53	.	.	PROPN
ajlm-658	3	54	2017	2017	NUM
ajlm-658	3	55	;	;	PUNCT
ajlm-658	3	56	accepted	accept	VERB
ajlm-658	3	57	:	:	PUNCT
ajlm-658	3	58	17	17	NUM
ajlm-658	3	59	oct	oct	PROPN
ajlm-658	3	60	.	.	PROPN
ajlm-658	3	61	2017	2017	NUM
ajlm-658	3	62	;	;	PUNCT
ajlm-658	3	63	published	publish	VERB
ajlm-658	3	64	:	:	PUNCT
ajlm-658	3	65	31	31	NUM
ajlm-658	3	66	jan	jan	PROPN
ajlm-658	3	67	.	.	PROPN
ajlm-658	4	1	2018	2018	NUM
ajlm-658	4	2	copyright	copyright	NOUN
ajlm-658	4	3	:	:	PUNCT
ajlm-658	4	4	©	©	PROPN
ajlm-658	4	5	2018	2018	NUM
ajlm-658	4	6	.	.	PUNCT
ajlm-658	5	1	the	the	DET
ajlm-658	5	2	author(s	author(s	PROPN
ajlm-658	5	3	)	)	PUNCT
ajlm-658	5	4	.	.	PUNCT
ajlm-658	6	1	licensee	licensee	NOUN
ajlm-658	6	2	:	:	PUNCT
ajlm-658	6	3	aosis	aosis	NOUN
ajlm-658	6	4	.	.	PUNCT
ajlm-658	7	1	this	this	PRON
ajlm-658	7	2	is	be	AUX
ajlm-658	7	3	an	an	DET
ajlm-658	7	4	open	open	ADJ
ajlm-658	7	5	access	access	NOUN
ajlm-658	7	6	article	article	NOUN
ajlm-658	7	7	distributed	distribute	VERB
ajlm-658	7	8	under	under	ADP
ajlm-658	7	9	the	the	DET
ajlm-658	7	10	terms	term	NOUN
ajlm-658	7	11	of	of	ADP
ajlm-658	7	12	the	the	DET
ajlm-658	7	13	creative	creative	ADJ
ajlm-658	7	14	commons	common	NOUN
ajlm-658	7	15	attribution	attribution	NOUN
ajlm-658	7	16	license	license	NOUN
ajlm-658	7	17	,	,	PUNCT
ajlm-658	7	18	which	which	PRON
ajlm-658	7	19	permits	permit	VERB
ajlm-658	7	20	unrestricted	unrestricted	ADJ
ajlm-658	7	21	use	use	NOUN
ajlm-658	7	22	,	,	PUNCT
ajlm-658	7	23	distribution	distribution	NOUN
ajlm-658	7	24	,	,	PUNCT
ajlm-658	7	25	and	and	CCONJ
ajlm-658	7	26	reproduction	reproduction	NOUN
ajlm-658	7	27	in	in	ADP
ajlm-658	7	28	any	any	DET
ajlm-658	7	29	medium	medium	NOUN
ajlm-658	7	30	,	,	PUNCT
ajlm-658	7	31	provided	provide	VERB
ajlm-658	7	32	the	the	DET
ajlm-658	7	33	original	original	ADJ
ajlm-658	7	34	work	work	NOUN
ajlm-658	7	35	is	be	AUX
ajlm-658	7	36	properly	properly	ADV
ajlm-658	7	37	cited	cite	VERB
ajlm-658	7	38	.	.	PUNCT
ajlm-658	8	1	abstract	abstract	ADJ
ajlm-658	8	2	the	the	DET
ajlm-658	8	3	purpose	purpose	NOUN
ajlm-658	8	4	of	of	ADP
ajlm-658	8	5	this	this	DET
ajlm-658	8	6	study	study	NOUN
ajlm-658	8	7	was	be	AUX
ajlm-658	8	8	to	to	PART
ajlm-658	8	9	develop	develop	VERB
ajlm-658	8	10	a	a	DET
ajlm-658	8	11	real	real	ADJ
ajlm-658	8	12	time	time	NOUN
ajlm-658	8	13	polymerase	polymerase	NOUN
ajlm-658	8	14	chain	chain	NOUN
ajlm-658	8	15	reaction	reaction	NOUN
ajlm-658	8	16	(	(	PUNCT
ajlm-658	8	17	pcr	pcr	NOUN
ajlm-658	8	18	)	)	PUNCT
ajlm-658	8	19	assay	assay	VERB
ajlm-658	8	20	for	for	ADP
ajlm-658	8	21	the	the	DET
ajlm-658	8	22	detection	detection	NOUN
ajlm-658	8	23	of	of	ADP
ajlm-658	8	24	the	the	DET
ajlm-658	8	25	jak2	jak2	PROPN
ajlm-658	8	26	v617f	v617f	PROPN
ajlm-658	8	27	mutation	mutation	NOUN
ajlm-658	8	28	that	that	PRON
ajlm-658	8	29	could	could	AUX
ajlm-658	8	30	be	be	AUX
ajlm-658	8	31	used	use	VERB
ajlm-658	8	32	in	in	ADP
ajlm-658	8	33	diagnostic	diagnostic	ADJ
ajlm-658	8	34	laboratories	laboratory	NOUN
ajlm-658	8	35	.	.	PUNCT
ajlm-658	9	1	sanger	sanger	PROPN
ajlm-658	9	2	sequencing	sequencing	NOUN
ajlm-658	9	3	and	and	CCONJ
ajlm-658	9	4	a	a	DET
ajlm-658	9	5	newly	newly	ADV
ajlm-658	9	6	developed	develop	VERB
ajlm-658	9	7	locked	locked	ADJ
ajlm-658	9	8	nucleic	nucleic	ADJ
ajlm-658	9	9	-	-	PUNCT
ajlm-658	9	10	acid	acid	ADJ
ajlm-658	9	11	,	,	PUNCT
ajlm-658	9	12	real	real	ADJ
ajlm-658	9	13	-	-	PUNCT
ajlm-658	9	14	time	time	NOUN
ajlm-658	9	15	pcr	pcr	PROPN
ajlm-658	9	16	assay	assay	NOUN
ajlm-658	9	17	were	be	AUX
ajlm-658	9	18	used	use	VERB
ajlm-658	9	19	to	to	PART
ajlm-658	9	20	detect	detect	VERB
ajlm-658	9	21	the	the	DET
ajlm-658	9	22	jak2	jak2	PROPN
ajlm-658	9	23	v617f	v617f	PROPN
ajlm-658	9	24	mutation	mutation	NOUN
ajlm-658	9	25	.	.	PUNCT
ajlm-658	10	1	there	there	PRON
ajlm-658	10	2	was	be	VERB
ajlm-658	10	3	100	100	NUM
ajlm-658	10	4	%	%	NOUN
ajlm-658	10	5	agreement	agreement	NOUN
ajlm-658	10	6	between	between	ADP
ajlm-658	10	7	the	the	DET
ajlm-658	10	8	sequencing	sequence	VERB
ajlm-658	10	9	and	and	CCONJ
ajlm-658	10	10	pcr	pcr	ADJ
ajlm-658	10	11	analysis	analysis	NOUN
ajlm-658	10	12	.	.	PUNCT
ajlm-658	11	1	both	both	DET
ajlm-658	11	2	assays	assay	NOUN
ajlm-658	11	3	were	be	AUX
ajlm-658	11	4	able	able	ADJ
ajlm-658	11	5	to	to	PART
ajlm-658	11	6	detect	detect	VERB
ajlm-658	11	7	the	the	DET
ajlm-658	11	8	mutation	mutation	NOUN
ajlm-658	11	9	in	in	ADP
ajlm-658	11	10	all	all	DET
ajlm-658	11	11	24	24	NUM
ajlm-658	11	12	of	of	ADP
ajlm-658	11	13	the	the	DET
ajlm-658	11	14	60	60	NUM
ajlm-658	11	15	test	test	NOUN
ajlm-658	11	16	specimens	specimen	NOUN
ajlm-658	11	17	harbouring	harbour	VERB
ajlm-658	11	18	the	the	DET
ajlm-658	11	19	mutation	mutation	NOUN
ajlm-658	11	20	.	.	PUNCT
ajlm-658	12	1	introduction	introduction	NOUN
ajlm-658	12	2	myeloproliferative	myeloproliferative	ADJ
ajlm-658	12	3	neoplasms	neoplasm	NOUN
ajlm-658	12	4	(	(	PUNCT
ajlm-658	12	5	mpns	mpns	NOUN
ajlm-658	12	6	)	)	PUNCT
ajlm-658	12	7	are	be	AUX
ajlm-658	12	8	a	a	DET
ajlm-658	12	9	heterogeneous	heterogeneous	ADJ
ajlm-658	12	10	group	group	NOUN
ajlm-658	12	11	of	of	ADP
ajlm-658	12	12	clonal	clonal	ADJ
ajlm-658	12	13	disorders	disorder	NOUN
ajlm-658	12	14	that	that	PRON
ajlm-658	12	15	result	result	VERB
ajlm-658	12	16	from	from	ADP
ajlm-658	12	17	mutational	mutational	ADJ
ajlm-658	12	18	transformation	transformation	NOUN
ajlm-658	12	19	of	of	ADP
ajlm-658	12	20	a	a	DET
ajlm-658	12	21	haematopoietic	haematopoietic	ADJ
ajlm-658	12	22	stem	stem	NOUN
ajlm-658	12	23	cell.1	cell.1	PROPN
ajlm-658	12	24	this	this	DET
ajlm-658	12	25	transformation	transformation	NOUN
ajlm-658	12	26	of	of	ADP
ajlm-658	12	27	haematopoietic	haematopoietic	ADJ
ajlm-658	12	28	stem	stem	NOUN
ajlm-658	12	29	cells	cell	NOUN
ajlm-658	12	30	causes	cause	VERB
ajlm-658	12	31	uncontrolled	uncontrolled	ADJ
ajlm-658	12	32	proliferation	proliferation	NOUN
ajlm-658	12	33	of	of	ADP
ajlm-658	12	34	myeloid	myeloid	ADJ
ajlm-658	12	35	cell	cell	NOUN
ajlm-658	12	36	lines	line	NOUN
ajlm-658	12	37	leading	lead	VERB
ajlm-658	12	38	to	to	ADP
ajlm-658	12	39	overproduction	overproduction	NOUN
ajlm-658	12	40	of	of	ADP
ajlm-658	12	41	both	both	CCONJ
ajlm-658	12	42	mature	mature	ADJ
ajlm-658	12	43	and	and	CCONJ
ajlm-658	12	44	immature	immature	ADJ
ajlm-658	12	45	blood	blood	NOUN
ajlm-658	12	46	cells.1	cells.1	NOUN
ajlm-658	12	47	prior	prior	ADV
ajlm-658	12	48	to	to	ADP
ajlm-658	12	49	2005	2005	NUM
ajlm-658	12	50	,	,	PUNCT
ajlm-658	12	51	no	no	DET
ajlm-658	12	52	underlying	underlie	VERB
ajlm-658	12	53	genetic	genetic	ADJ
ajlm-658	12	54	abnormality	abnormality	NOUN
ajlm-658	12	55	had	have	AUX
ajlm-658	12	56	been	be	AUX
ajlm-658	12	57	associated	associate	VERB
ajlm-658	12	58	with	with	ADP
ajlm-658	12	59	classic	classic	ADJ
ajlm-658	12	60	breakpoint	breakpoint	NOUN
ajlm-658	12	61	cluster	cluster	NOUN
ajlm-658	12	62	region	region	NOUN
ajlm-658	12	63	-	-	PUNCT
ajlm-658	12	64	abelson	abelson	NOUN
ajlm-658	12	65	leukaemia	leukaemia	PROPN
ajlm-658	12	66	virus	virus	PROPN
ajlm-658	12	67	negative	negative	ADJ
ajlm-658	12	68	mpns	mpns	NOUN
ajlm-658	12	69	.	.	PUNCT
ajlm-658	13	1	in	in	ADP
ajlm-658	13	2	2005	2005	NUM
ajlm-658	13	3	,	,	PUNCT
ajlm-658	13	4	a	a	DET
ajlm-658	13	5	mutation	mutation	NOUN
ajlm-658	13	6	at	at	ADP
ajlm-658	13	7	base	base	NOUN
ajlm-658	13	8	1849	1849	NUM
ajlm-658	13	9	in	in	ADP
ajlm-658	13	10	exon	exon	PROPN
ajlm-658	13	11	14	14	NUM
ajlm-658	13	12	of	of	ADP
ajlm-658	13	13	the	the	DET
ajlm-658	13	14	jak2	jak2	PROPN
ajlm-658	13	15	gene	gene	NOUN
ajlm-658	13	16	on	on	ADP
ajlm-658	13	17	chromosome	chromosome	NOUN
ajlm-658	13	18	9	9	NUM
ajlm-658	13	19	was	be	AUX
ajlm-658	13	20	discovered	discover	VERB
ajlm-658	13	21	in	in	ADP
ajlm-658	13	22	patients	patient	NOUN
ajlm-658	13	23	with	with	ADP
ajlm-658	13	24	polycythaema	polycythaema	NOUN
ajlm-658	13	25	vera	vera	NOUN
ajlm-658	13	26	,	,	PUNCT
ajlm-658	13	27	essential	essential	ADJ
ajlm-658	13	28	thrombocythaemia	thrombocythaemia	NOUN
ajlm-658	13	29	and	and	CCONJ
ajlm-658	13	30	primary	primary	ADJ
ajlm-658	13	31	myelofibrosis.2,3,4	myelofibrosis.2,3,4	NOUN
ajlm-658	13	32	this	this	DET
ajlm-658	13	33	somatic	somatic	ADJ
ajlm-658	13	34	point	point	NOUN
ajlm-658	13	35	mutation	mutation	NOUN
ajlm-658	13	36	causes	cause	VERB
ajlm-658	13	37	a	a	DET
ajlm-658	13	38	substitution	substitution	NOUN
ajlm-658	13	39	of	of	ADP
ajlm-658	13	40	guanine	guanine	NOUN
ajlm-658	13	41	by	by	ADP
ajlm-658	13	42	thymine	thymine	PROPN
ajlm-658	13	43	and	and	CCONJ
ajlm-658	13	44	the	the	DET
ajlm-658	13	45	amino	amino	NOUN
ajlm-658	13	46	acid	acid	NOUN
ajlm-658	13	47	is	be	AUX
ajlm-658	13	48	changed	change	VERB
ajlm-658	13	49	from	from	ADP
ajlm-658	13	50	valine	valine	NOUN
ajlm-658	13	51	to	to	ADP
ajlm-658	13	52	phenylalanine	phenylalanine	NOUN
ajlm-658	13	53	in	in	ADP
ajlm-658	13	54	codon	codon	PROPN
ajlm-658	13	55	617	617	NUM
ajlm-658	13	56	of	of	ADP
ajlm-658	13	57	the	the	DET
ajlm-658	13	58	jak2	jak2	NOUN
ajlm-658	13	59	protein.2	protein.2	PROPN
ajlm-658	13	60	this	this	DET
ajlm-658	13	61	discovery	discovery	NOUN
ajlm-658	13	62	has	have	AUX
ajlm-658	13	63	improved	improve	VERB
ajlm-658	13	64	understanding	understanding	NOUN
ajlm-658	13	65	of	of	ADP
ajlm-658	13	66	the	the	DET
ajlm-658	13	67	pathophysiology	pathophysiology	NOUN
ajlm-658	13	68	of	of	ADP
ajlm-658	13	69	mpns	mpns	NOUN
ajlm-658	13	70	and	and	CCONJ
ajlm-658	13	71	has	have	AUX
ajlm-658	13	72	renewed	renew	VERB
ajlm-658	13	73	interest	interest	NOUN
ajlm-658	13	74	and	and	CCONJ
ajlm-658	13	75	research	research	NOUN
ajlm-658	13	76	in	in	ADP
ajlm-658	13	77	mpn	mpn	NOUN
ajlm-658	13	78	biology	biology	NOUN
ajlm-658	13	79	and	and	CCONJ
ajlm-658	13	80	genetics.5,6	genetics.5,6	VERB
ajlm-658	13	81	the	the	DET
ajlm-658	13	82	ability	ability	NOUN
ajlm-658	13	83	to	to	PART
ajlm-658	13	84	demonstrate	demonstrate	VERB
ajlm-658	13	85	the	the	DET
ajlm-658	13	86	presence	presence	NOUN
ajlm-658	13	87	of	of	ADP
ajlm-658	13	88	this	this	DET
ajlm-658	13	89	mutation	mutation	NOUN
ajlm-658	13	90	has	have	AUX
ajlm-658	13	91	not	not	PART
ajlm-658	13	92	only	only	ADV
ajlm-658	13	93	simplified	simplify	VERB
ajlm-658	13	94	the	the	DET
ajlm-658	13	95	diagnosis	diagnosis	NOUN
ajlm-658	13	96	of	of	ADP
ajlm-658	13	97	mpn	mpn	NOUN
ajlm-658	13	98	but	but	CCONJ
ajlm-658	13	99	also	also	ADV
ajlm-658	13	100	ensured	ensure	VERB
ajlm-658	13	101	greater	great	ADJ
ajlm-658	13	102	diagnostic	diagnostic	ADJ
ajlm-658	13	103	accuracy.5	accuracy.5	PROPN
ajlm-658	13	104	the	the	DET
ajlm-658	13	105	identification	identification	NOUN
ajlm-658	13	106	of	of	ADP
ajlm-658	13	107	this	this	DET
ajlm-658	13	108	mutation	mutation	NOUN
ajlm-658	13	109	has	have	AUX
ajlm-658	13	110	also	also	ADV
ajlm-658	13	111	led	lead	VERB
ajlm-658	13	112	to	to	ADP
ajlm-658	13	113	the	the	DET
ajlm-658	13	114	development	development	NOUN
ajlm-658	13	115	of	of	ADP
ajlm-658	13	116	therapies	therapy	NOUN
ajlm-658	13	117	targeted	target	VERB
ajlm-658	13	118	at	at	ADP
ajlm-658	13	119	inhibition	inhibition	NOUN
ajlm-658	13	120	of	of	ADP
ajlm-658	13	121	the	the	DET
ajlm-658	13	122	jak2	jak2	NOUN
ajlm-658	13	123	kinase.7,8	kinase.7,8	ADP
ajlm-658	13	124	sensitive	sensitive	ADJ
ajlm-658	13	125	and	and	CCONJ
ajlm-658	13	126	specific	specific	ADJ
ajlm-658	13	127	assays	assay	NOUN
ajlm-658	13	128	are	be	AUX
ajlm-658	13	129	required	require	VERB
ajlm-658	13	130	for	for	ADP
ajlm-658	13	131	the	the	DET
ajlm-658	13	132	detection	detection	NOUN
ajlm-658	13	133	of	of	ADP
ajlm-658	13	134	the	the	DET
ajlm-658	13	135	jak2	jak2	PROPN
ajlm-658	13	136	v617f	v617f	PROPN
ajlm-658	13	137	mutation.9	mutation.9	PROPN
ajlm-658	13	138	molecular	molecular	ADJ
ajlm-658	13	139	assays	assay	NOUN
ajlm-658	13	140	with	with	ADP
ajlm-658	13	141	high	high	ADJ
ajlm-658	13	142	sensitivity	sensitivity	NOUN
ajlm-658	13	143	and	and	CCONJ
ajlm-658	13	144	specificity	specificity	NOUN
ajlm-658	13	145	should	should	AUX
ajlm-658	13	146	be	be	AUX
ajlm-658	13	147	offered	offer	VERB
ajlm-658	13	148	by	by	ADP
ajlm-658	13	149	diagnostic	diagnostic	ADJ
ajlm-658	13	150	laboratories	laboratory	NOUN
ajlm-658	13	151	for	for	ADP
ajlm-658	13	152	this	this	DET
ajlm-658	13	153	purpose.9	purpose.9	NOUN
ajlm-658	13	154	several	several	ADJ
ajlm-658	13	155	different	different	ADJ
ajlm-658	13	156	commercial	commercial	NOUN
ajlm-658	13	157	and	and	CCONJ
ajlm-658	13	158	in	in	ADP
ajlm-658	13	159	-	-	PUNCT
ajlm-658	13	160	house	house	NOUN
ajlm-658	13	161	assays	assay	NOUN
ajlm-658	13	162	that	that	PRON
ajlm-658	13	163	offer	offer	VERB
ajlm-658	13	164	different	different	ADJ
ajlm-658	13	165	sensitivity	sensitivity	NOUN
ajlm-658	13	166	and	and	CCONJ
ajlm-658	13	167	specificity	specificity	NOUN
ajlm-658	13	168	levels	level	NOUN
ajlm-658	13	169	have	have	AUX
ajlm-658	13	170	been	be	AUX
ajlm-658	13	171	developed.9	developed.9	ADP
ajlm-658	13	172	direct	direct	ADJ
ajlm-658	13	173	sequencing	sequencing	NOUN
ajlm-658	13	174	is	be	AUX
ajlm-658	13	175	a	a	DET
ajlm-658	13	176	gold	gold	ADJ
ajlm-658	13	177	standard	standard	NOUN
ajlm-658	13	178	for	for	ADP
ajlm-658	13	179	mutation	mutation	NOUN
ajlm-658	13	180	analysis	analysis	NOUN
ajlm-658	13	181	but	but	CCONJ
ajlm-658	13	182	is	be	AUX
ajlm-658	13	183	limited	limit	VERB
ajlm-658	13	184	by	by	ADP
ajlm-658	13	185	low	low	ADJ
ajlm-658	13	186	sensitivity	sensitivity	NOUN
ajlm-658	13	187	and	and	CCONJ
ajlm-658	13	188	high	high	ADJ
ajlm-658	13	189	cost.10	cost.10	NOUN
ajlm-658	13	190	in	in	ADP
ajlm-658	13	191	-	-	PUNCT
ajlm-658	13	192	house	house	NOUN
ajlm-658	13	193	assays	assay	NOUN
ajlm-658	13	194	that	that	PRON
ajlm-658	13	195	are	be	AUX
ajlm-658	13	196	currently	currently	ADV
ajlm-658	13	197	used	use	VERB
ajlm-658	13	198	include	include	VERB
ajlm-658	13	199	allele	allele	NOUN
ajlm-658	13	200	-	-	PUNCT
ajlm-658	13	201	specific	specific	ADJ
ajlm-658	13	202	polymerase	polymerase	NOUN
ajlm-658	13	203	chain	chain	NOUN
ajlm-658	13	204	reaction	reaction	NOUN
ajlm-658	13	205	or	or	CCONJ
ajlm-658	13	206	amplification	amplification	NOUN
ajlm-658	13	207	refractory	refractory	NOUN
ajlm-658	13	208	mutation	mutation	NOUN
ajlm-658	13	209	system	system	NOUN
ajlm-658	13	210	,	,	PUNCT
ajlm-658	13	211	direct	direct	ADJ
ajlm-658	13	212	sequencing	sequencing	NOUN
ajlm-658	13	213	,	,	PUNCT
ajlm-658	13	214	polymerase	polymerase	NOUN
ajlm-658	13	215	chain	chain	NOUN
ajlm-658	13	216	reaction	reaction	NOUN
ajlm-658	13	217	(	(	PUNCT
ajlm-658	13	218	pcr	pcr	PROPN
ajlm-658	13	219	)	)	PUNCT
ajlm-658	13	220	with	with	ADP
ajlm-658	13	221	restriction	restriction	NOUN
ajlm-658	13	222	fragment	fragment	NOUN
ajlm-658	13	223	length	length	NOUN
ajlm-658	13	224	polymorphism	polymorphism	NOUN
ajlm-658	13	225	(	(	PUNCT
ajlm-658	13	226	pcr	pcr	NOUN
ajlm-658	13	227	-	-	PUNCT
ajlm-658	13	228	rflp	rflp	NOUN
ajlm-658	13	229	)	)	PUNCT
ajlm-658	13	230	and	and	CCONJ
ajlm-658	13	231	real	real	ADJ
ajlm-658	13	232	-	-	PUNCT
ajlm-658	13	233	time	time	NOUN
ajlm-658	13	234	pcr.9,11,12	pcr.9,11,12	ADJ
ajlm-658	13	235	diagnostic	diagnostic	ADJ
ajlm-658	13	236	laboratories	laboratory	NOUN
ajlm-658	13	237	may	may	AUX
ajlm-658	13	238	find	find	VERB
ajlm-658	13	239	it	it	PRON
ajlm-658	13	240	challenging	challenge	VERB
ajlm-658	13	241	to	to	PART
ajlm-658	13	242	select	select	VERB
ajlm-658	13	243	the	the	DET
ajlm-658	13	244	most	most	ADV
ajlm-658	13	245	appropriate	appropriate	ADJ
ajlm-658	13	246	methodology	methodology	NOUN
ajlm-658	13	247	to	to	PART
ajlm-658	13	248	detect	detect	VERB
ajlm-658	13	249	the	the	DET
ajlm-658	13	250	jak2	jak2	PROPN
ajlm-658	13	251	mutation.13	mutation.13	PROPN
ajlm-658	13	252	however	however	ADV
ajlm-658	13	253	,	,	PUNCT
ajlm-658	13	254	the	the	DET
ajlm-658	13	255	use	use	NOUN
ajlm-658	13	256	of	of	ADP
ajlm-658	13	257	a	a	DET
ajlm-658	13	258	reliable	reliable	ADJ
ajlm-658	13	259	,	,	PUNCT
ajlm-658	13	260	quick	quick	ADJ
ajlm-658	13	261	and	and	CCONJ
ajlm-658	13	262	sensitive	sensitive	ADJ
ajlm-658	13	263	assay	assay	NOUN
ajlm-658	13	264	that	that	PRON
ajlm-658	13	265	is	be	AUX
ajlm-658	13	266	able	able	ADJ
ajlm-658	13	267	to	to	PART
ajlm-658	13	268	detect	detect	VERB
ajlm-658	13	269	1	1	NUM
ajlm-658	13	270	%	%	NOUN
ajlm-658	13	271	of	of	ADP
ajlm-658	13	272	the	the	DET
ajlm-658	13	273	mutant	mutant	ADJ
ajlm-658	13	274	allele	allele	NOUN
ajlm-658	13	275	in	in	ADP
ajlm-658	13	276	a	a	DET
ajlm-658	13	277	wild	wild	ADJ
ajlm-658	13	278	-	-	PUNCT
ajlm-658	13	279	type	type	NOUN
ajlm-658	13	280	background	background	NOUN
ajlm-658	13	281	is	be	AUX
ajlm-658	13	282	recommended.13	recommended.13	PRON
ajlm-658	13	283	the	the	DET
ajlm-658	13	284	aim	aim	NOUN
ajlm-658	13	285	of	of	ADP
ajlm-658	13	286	this	this	DET
ajlm-658	13	287	study	study	NOUN
ajlm-658	13	288	was	be	AUX
ajlm-658	13	289	to	to	PART
ajlm-658	13	290	develop	develop	VERB
ajlm-658	13	291	a	a	DET
ajlm-658	13	292	real	real	ADJ
ajlm-658	13	293	-	-	PUNCT
ajlm-658	13	294	time	time	NOUN
ajlm-658	13	295	pcr	pcr	NOUN
ajlm-658	13	296	assay	assay	NOUN
ajlm-658	13	297	for	for	ADP
ajlm-658	13	298	the	the	DET
ajlm-658	13	299	detection	detection	NOUN
ajlm-658	13	300	of	of	ADP
ajlm-658	13	301	the	the	DET
ajlm-658	13	302	jak2	jak2	PROPN
ajlm-658	13	303	v617f	v617f	PROPN
ajlm-658	13	304	mutation	mutation	NOUN
ajlm-658	13	305	for	for	ADP
ajlm-658	13	306	implementation	implementation	NOUN
ajlm-658	13	307	in	in	ADP
ajlm-658	13	308	a	a	DET
ajlm-658	13	309	routine	routine	ADJ
ajlm-658	13	310	clinical	clinical	ADJ
ajlm-658	13	311	laboratory	laboratory	NOUN
ajlm-658	13	312	.	.	PUNCT
ajlm-658	14	1	methods	method	NOUN
ajlm-658	14	2	ethical	ethical	ADJ
ajlm-658	14	3	considerations	consideration	NOUN
ajlm-658	14	4	the	the	DET
ajlm-658	14	5	study	study	NOUN
ajlm-658	14	6	received	receive	VERB
ajlm-658	14	7	approval	approval	NOUN
ajlm-658	14	8	from	from	ADP
ajlm-658	14	9	the	the	DET
ajlm-658	14	10	university	university	PROPN
ajlm-658	14	11	of	of	ADP
ajlm-658	14	12	pretoria	pretoria	PROPN
ajlm-658	14	13	faculty	faculty	PROPN
ajlm-658	14	14	of	of	ADP
ajlm-658	14	15	health	health	PROPN
ajlm-658	14	16	sciences	sciences	PROPN
ajlm-658	14	17	research	research	PROPN
ajlm-658	14	18	ethics	ethic	NOUN
ajlm-658	14	19	committee	committee	PROPN
ajlm-658	14	20	(	(	PUNCT
ajlm-658	14	21	s32/2012	s32/2012	NOUN
ajlm-658	14	22	)	)	PUNCT
ajlm-658	14	23	for	for	ADP
ajlm-658	14	24	using	use	VERB
ajlm-658	14	25	sample	sample	NOUN
ajlm-658	14	26	remnants	remnant	NOUN
ajlm-658	14	27	for	for	ADP
ajlm-658	14	28	further	further	ADJ
ajlm-658	14	29	analysis	analysis	NOUN
ajlm-658	14	30	.	.	PUNCT
ajlm-658	15	1	leftover	leftover	NOUN
ajlm-658	15	2	blood	blood	NOUN
ajlm-658	15	3	samples	sample	NOUN
ajlm-658	15	4	were	be	AUX
ajlm-658	15	5	collected	collect	VERB
ajlm-658	15	6	anonymously	anonymously	ADV
ajlm-658	15	7	from	from	ADP
ajlm-658	15	8	a	a	DET
ajlm-658	15	9	tshwane	tshwane	NOUN
ajlm-658	15	10	tertiary	tertiary	ADJ
ajlm-658	15	11	hospital	hospital	NOUN
ajlm-658	15	12	for	for	ADP
ajlm-658	15	13	dna	dna	NOUN
ajlm-658	15	14	extraction	extraction	NOUN
ajlm-658	15	15	and	and	CCONJ
ajlm-658	15	16	pcr	pcr	NOUN
ajlm-658	15	17	assay	assay	NOUN
ajlm-658	15	18	.	.	PUNCT
ajlm-658	16	1	all	all	DET
ajlm-658	16	2	samples	sample	NOUN
ajlm-658	16	3	were	be	AUX
ajlm-658	16	4	used	use	VERB
ajlm-658	16	5	solely	solely	ADV
ajlm-658	16	6	for	for	ADP
ajlm-658	16	7	the	the	DET
ajlm-658	16	8	detection	detection	NOUN
ajlm-658	16	9	of	of	ADP
ajlm-658	16	10	the	the	DET
ajlm-658	16	11	jak2	jak2	PROPN
ajlm-658	16	12	v617f	v617f	PROPN
ajlm-658	16	13	mutation	mutation	NOUN
ajlm-658	16	14	.	.	PUNCT
ajlm-658	17	1	samples	sample	NOUN
ajlm-658	17	2	collected	collect	VERB
ajlm-658	17	3	and	and	CCONJ
ajlm-658	17	4	study	study	NOUN
ajlm-658	17	5	site	site	NOUN
ajlm-658	17	6	this	this	DET
ajlm-658	17	7	study	study	NOUN
ajlm-658	17	8	used	use	VERB
ajlm-658	17	9	specimens	specimen	NOUN
ajlm-658	17	10	of	of	ADP
ajlm-658	17	11	whole	whole	ADJ
ajlm-658	17	12	blood	blood	NOUN
ajlm-658	17	13	collected	collect	VERB
ajlm-658	17	14	in	in	ADP
ajlm-658	17	15	edta	edta	NOUN
ajlm-658	17	16	(	(	PUNCT
ajlm-658	17	17	n	n	NOUN
ajlm-658	17	18	=	=	SYM
ajlm-658	17	19	60	60	NUM
ajlm-658	17	20	)	)	PUNCT
ajlm-658	17	21	that	that	PRON
ajlm-658	17	22	were	be	AUX
ajlm-658	17	23	submitted	submit	VERB
ajlm-658	17	24	by	by	ADP
ajlm-658	17	25	clinicians	clinician	NOUN
ajlm-658	17	26	from	from	ADP
ajlm-658	17	27	steve	steve	PROPN
ajlm-658	17	28	biko	biko	PROPN
ajlm-658	17	29	academic	academic	ADJ
ajlm-658	17	30	hospital	hospital	NOUN
ajlm-658	17	31	to	to	PART
ajlm-658	17	32	be	be	AUX
ajlm-658	17	33	evaluated	evaluate	VERB
ajlm-658	17	34	for	for	ADP
ajlm-658	17	35	the	the	DET
ajlm-658	17	36	presence	presence	NOUN
ajlm-658	17	37	of	of	ADP
ajlm-658	17	38	the	the	DET
ajlm-658	17	39	jak2	jak2	PROPN
ajlm-658	17	40	v617f	v617f	PROPN
ajlm-658	17	41	mutation	mutation	NOUN
ajlm-658	17	42	in	in	ADP
ajlm-658	17	43	patients	patient	NOUN
ajlm-658	17	44	suspected	suspect	VERB
ajlm-658	17	45	to	to	PART
ajlm-658	17	46	have	have	VERB
ajlm-658	17	47	mpns	mpns	NOUN
ajlm-658	17	48	.	.	PUNCT
ajlm-658	18	1	the	the	DET
ajlm-658	18	2	study	study	NOUN
ajlm-658	18	3	was	be	AUX
ajlm-658	18	4	conducted	conduct	VERB
ajlm-658	18	5	between	between	ADP
ajlm-658	18	6	01	01	NUM
ajlm-658	18	7	october	october	PROPN
ajlm-658	18	8	2013	2013	NUM
ajlm-658	18	9	and	and	CCONJ
ajlm-658	18	10	30	30	NUM
ajlm-658	18	11	september	september	PROPN
ajlm-658	18	12	2015	2015	NUM
ajlm-658	18	13	.	.	PUNCT
ajlm-658	19	1	genomic	genomic	ADJ
ajlm-658	19	2	dna	dna	PROPN
ajlm-658	19	3	genomic	genomic	NOUN
ajlm-658	19	4	dna	dna	PROPN
ajlm-658	19	5	(	(	PUNCT
ajlm-658	19	6	gdna	gdna	ADV
ajlm-658	19	7	)	)	PUNCT
ajlm-658	19	8	was	be	AUX
ajlm-658	19	9	extracted	extract	VERB
ajlm-658	19	10	from	from	ADP
ajlm-658	19	11	0.2	0.2	NUM
ajlm-658	19	12	ml	ml	NOUN
ajlm-658	19	13	of	of	ADP
ajlm-658	19	14	peripheral	peripheral	ADJ
ajlm-658	19	15	blood	blood	NOUN
ajlm-658	19	16	using	use	VERB
ajlm-658	19	17	the	the	DET
ajlm-658	19	18	genelute	genelute	NOUN
ajlm-658	19	19	™	™	ADJ
ajlm-658	19	20	blood	blood	NOUN
ajlm-658	19	21	genomic	genomic	NOUN
ajlm-658	19	22	dna	dna	PROPN
ajlm-658	19	23	kit	kit	NOUN
ajlm-658	19	24	(	(	PUNCT
ajlm-658	19	25	sigma	sigma	PROPN
ajlm-658	19	26	aldrich	aldrich	PROPN
ajlm-658	19	27	,	,	PUNCT
ajlm-658	19	28	st	st	PROPN
ajlm-658	19	29	.	.	PROPN
ajlm-658	19	30	louis	louis	PROPN
ajlm-658	19	31	,	,	PUNCT
ajlm-658	19	32	missouri	missouri	PROPN
ajlm-658	19	33	,	,	PUNCT
ajlm-658	19	34	united	united	PROPN
ajlm-658	19	35	states	states	PROPN
ajlm-658	19	36	)	)	PUNCT
ajlm-658	19	37	according	accord	VERB
ajlm-658	19	38	to	to	ADP
ajlm-658	19	39	the	the	DET
ajlm-658	19	40	manufacturer	manufacturer	NOUN
ajlm-658	19	41	’s	’s	PART
ajlm-658	19	42	instructions	instruction	NOUN
ajlm-658	19	43	.	.	PUNCT
ajlm-658	20	1	the	the	DET
ajlm-658	20	2	concentration	concentration	NOUN
ajlm-658	20	3	of	of	ADP
ajlm-658	20	4	dna	dna	PROPN
ajlm-658	20	5	was	be	AUX
ajlm-658	20	6	confirmed	confirm	VERB
ajlm-658	20	7	by	by	ADP
ajlm-658	20	8	the	the	DET
ajlm-658	20	9	nanodrop	nanodrop	NOUN
ajlm-658	20	10	2000c	2000c	NUM
ajlm-658	20	11	uv	uv	NOUN
ajlm-658	20	12	spectrophotometer	spectrophotometer	NOUN
ajlm-658	20	13	(	(	PUNCT
ajlm-658	20	14	thermo	thermo	NOUN
ajlm-658	20	15	scientific	scientific	NOUN
ajlm-658	20	16	,	,	PUNCT
ajlm-658	20	17	waltham	waltham	PROPN
ajlm-658	20	18	,	,	PUNCT
ajlm-658	20	19	massachusetts	massachusetts	PROPN
ajlm-658	20	20	,	,	PUNCT
ajlm-658	20	21	united	united	PROPN
ajlm-658	20	22	states	states	PROPN
ajlm-658	20	23	)	)	PUNCT
ajlm-658	20	24	to	to	PART
ajlm-658	20	25	obtain	obtain	VERB
ajlm-658	20	26	gdna	gdna	ADV
ajlm-658	20	27	with	with	ADP
ajlm-658	20	28	a	a	DET
ajlm-658	20	29	concentration	concentration	NOUN
ajlm-658	20	30	of	of	ADP
ajlm-658	20	31	1.6	1.6	NUM
ajlm-658	20	32	to	to	PART
ajlm-658	20	33	1.9	1.9	NUM
ajlm-658	20	34	µg	µg	NOUN
ajlm-658	20	35	/	/	SYM
ajlm-658	20	36	ml	ml	NOUN
ajlm-658	20	37	.	.	VERB
ajlm-658	21	1	extracted	extract	VERB
ajlm-658	21	2	dna	dna	PROPN
ajlm-658	21	3	was	be	AUX
ajlm-658	21	4	stored	store	VERB
ajlm-658	21	5	at	at	ADP
ajlm-658	21	6	–	–	PUNCT
ajlm-658	21	7	20	20	NUM
ajlm-658	21	8	°	°	NUM
ajlm-658	21	9	c	c	NOUN
ajlm-658	21	10	until	until	ADP
ajlm-658	21	11	analysis	analysis	NOUN
ajlm-658	21	12	.	.	PUNCT
ajlm-658	22	1	sequencing	sequence	VERB
ajlm-658	22	2	of	of	ADP
ajlm-658	22	3	amplified	amplify	VERB
ajlm-658	22	4	products	product	NOUN
ajlm-658	22	5	primers	primer	NOUN
ajlm-658	22	6	for	for	ADP
ajlm-658	22	7	the	the	DET
ajlm-658	22	8	detection	detection	NOUN
ajlm-658	22	9	of	of	ADP
ajlm-658	22	10	the	the	DET
ajlm-658	22	11	single	single	ADJ
ajlm-658	22	12	nucleotide	nucleotide	ADJ
ajlm-658	22	13	polymorphism	polymorphism	NOUN
ajlm-658	22	14	in	in	ADP
ajlm-658	22	15	codon	codon	PROPN
ajlm-658	22	16	617	617	NUM
ajlm-658	22	17	were	be	AUX
ajlm-658	22	18	designed	design	VERB
ajlm-658	22	19	based	base	VERB
ajlm-658	22	20	on	on	ADP
ajlm-658	22	21	the	the	DET
ajlm-658	22	22	known	know	VERB
ajlm-658	22	23	dna	dna	NOUN
ajlm-658	22	24	sequence	sequence	NOUN
ajlm-658	22	25	of	of	ADP
ajlm-658	22	26	the	the	DET
ajlm-658	22	27	jak2	jak2	PROPN
ajlm-658	22	28	gene	gene	NOUN
ajlm-658	22	29	(	(	PUNCT
ajlm-658	22	30	genbank	genbank	NOUN
ajlm-658	22	31	®	®	NOUN
ajlm-658	22	32	accession	accession	NOUN
ajlm-658	22	33	number	number	NOUN
ajlm-658	22	34	ng_009904.1	ng_009904.1	ADV
ajlm-658	22	35	)	)	PUNCT
ajlm-658	22	36	using	use	VERB
ajlm-658	22	37	the	the	DET
ajlm-658	22	38	primerquest	primerquest	NOUN
ajlm-658	22	39	software	software	NOUN
ajlm-658	22	40	(	(	PUNCT
ajlm-658	22	41	integrated	integrate	VERB
ajlm-658	22	42	dna	dna	PROPN
ajlm-658	22	43	technologies	technology	NOUN
ajlm-658	22	44	,	,	PUNCT
ajlm-658	22	45	coralville	coralville	PROPN
ajlm-658	22	46	,	,	PUNCT
ajlm-658	22	47	iowa	iowa	PROPN
ajlm-658	22	48	,	,	PUNCT
ajlm-658	22	49	united	united	PROPN
ajlm-658	22	50	states	states	PROPN
ajlm-658	22	51	)	)	PUNCT
ajlm-658	22	52	(	(	PUNCT
ajlm-658	22	53	table	table	NOUN
ajlm-658	22	54	1	1	NUM
ajlm-658	22	55	)	)	PUNCT
ajlm-658	22	56	.	.	PUNCT
ajlm-658	23	1	the	the	DET
ajlm-658	23	2	product	product	NOUN
ajlm-658	23	3	of	of	ADP
ajlm-658	23	4	interest	interest	NOUN
ajlm-658	23	5	was	be	AUX
ajlm-658	23	6	amplified	amplify	VERB
ajlm-658	23	7	using	use	VERB
ajlm-658	23	8	the	the	DET
ajlm-658	23	9	dna	dna	PROPN
ajlm-658	23	10	engine	engine	NOUN
ajlm-658	23	11	peltier	peltier	NOUN
ajlm-658	23	12	thermocycler	thermocycler	NOUN
ajlm-658	23	13	(	(	PUNCT
ajlm-658	23	14	bio	bio	PROPN
ajlm-658	23	15	rad	rad	PROPN
ajlm-658	23	16	,	,	PUNCT
ajlm-658	23	17	münchen	münchen	PROPN
ajlm-658	23	18	,	,	PUNCT
ajlm-658	23	19	germany	germany	PROPN
ajlm-658	23	20	)	)	PUNCT
ajlm-658	23	21	under	under	ADP
ajlm-658	23	22	the	the	DET
ajlm-658	23	23	following	following	ADJ
ajlm-658	23	24	conditions	condition	NOUN
ajlm-658	23	25	:	:	PUNCT
ajlm-658	23	26	initial	initial	ADJ
ajlm-658	23	27	denaturation	denaturation	NOUN
ajlm-658	23	28	at	at	ADP
ajlm-658	23	29	94	94	NUM
ajlm-658	23	30	°	°	ADP
ajlm-658	23	31	c	c	NOUN
ajlm-658	23	32	for	for	ADP
ajlm-658	23	33	10	10	NUM
ajlm-658	23	34	minutes	minute	NOUN
ajlm-658	23	35	,	,	PUNCT
ajlm-658	23	36	followed	follow	VERB
ajlm-658	23	37	by	by	ADP
ajlm-658	23	38	35	35	NUM
ajlm-658	23	39	cycles	cycle	NOUN
ajlm-658	23	40	of	of	ADP
ajlm-658	23	41	amplification	amplification	NOUN
ajlm-658	23	42	with	with	ADP
ajlm-658	23	43	denaturation	denaturation	NOUN
ajlm-658	23	44	at	at	ADP
ajlm-658	23	45	94	94	NUM
ajlm-658	23	46	°	°	ADP
ajlm-658	23	47	c	c	NOUN
ajlm-658	23	48	for	for	ADP
ajlm-658	23	49	30	30	NUM
ajlm-658	23	50	seconds	second	NOUN
ajlm-658	23	51	,	,	PUNCT
ajlm-658	23	52	annealing	anneal	VERB
ajlm-658	23	53	at	at	ADP
ajlm-658	23	54	52	52	NUM
ajlm-658	23	55	°	°	NOUN
ajlm-658	23	56	c	c	NOUN
ajlm-658	23	57	for	for	ADP
ajlm-658	23	58	30	30	NUM
ajlm-658	23	59	seconds	second	NOUN
ajlm-658	23	60	and	and	CCONJ
ajlm-658	23	61	extension	extension	NOUN
ajlm-658	23	62	at	at	ADP
ajlm-658	23	63	72	72	NUM
ajlm-658	23	64	°	°	ADP
ajlm-658	23	65	c	c	NOUN
ajlm-658	23	66	for	for	ADP
ajlm-658	23	67	45	45	NUM
ajlm-658	23	68	seconds	second	NOUN
ajlm-658	23	69	.	.	PUNCT
ajlm-658	24	1	sanger	sanger	PROPN
ajlm-658	24	2	sequencing	sequencing	NOUN
ajlm-658	24	3	was	be	AUX
ajlm-658	24	4	performed	perform	VERB
ajlm-658	24	5	by	by	ADP
ajlm-658	24	6	inqaba	inqaba	NOUN
ajlm-658	24	7	biotechnical	biotechnical	ADJ
ajlm-658	24	8	industries	industry	NOUN
ajlm-658	24	9	(	(	PUNCT
ajlm-658	24	10	pretoria	pretoria	PROPN
ajlm-658	24	11	,	,	PUNCT
ajlm-658	24	12	south	south	PROPN
ajlm-658	24	13	africa	africa	PROPN
ajlm-658	24	14	)	)	PUNCT
ajlm-658	24	15	in	in	ADP
ajlm-658	24	16	both	both	DET
ajlm-658	24	17	directions	direction	NOUN
ajlm-658	24	18	on	on	ADP
ajlm-658	24	19	all	all	DET
ajlm-658	24	20	60	60	NUM
ajlm-658	24	21	amplified	amplified	ADJ
ajlm-658	24	22	products	product	NOUN
ajlm-658	24	23	.	.	PUNCT
ajlm-658	25	1	the	the	DET
ajlm-658	25	2	clc	clc	PROPN
ajlm-658	25	3	main	main	ADJ
ajlm-658	25	4	workbench	workbench	NOUN
ajlm-658	25	5	software	software	NOUN
ajlm-658	25	6	program	program	NOUN
ajlm-658	25	7	v6.0	v6.0	PROPN
ajlm-658	25	8	(	(	PUNCT
ajlm-658	25	9	clcbio	clcbio	NOUN
ajlm-658	25	10	,	,	PUNCT
ajlm-658	25	11	waltham	waltham	PROPN
ajlm-658	25	12	,	,	PUNCT
ajlm-658	25	13	massachusetts	massachusetts	PROPN
ajlm-658	25	14	,	,	PUNCT
ajlm-658	25	15	united	united	PROPN
ajlm-658	25	16	states	states	PROPN
ajlm-658	25	17	)	)	PUNCT
ajlm-658	25	18	was	be	AUX
ajlm-658	25	19	used	use	VERB
ajlm-658	25	20	to	to	PART
ajlm-658	25	21	analyse	analyse	VERB
ajlm-658	25	22	the	the	DET
ajlm-658	25	23	sequences	sequence	NOUN
ajlm-658	25	24	against	against	ADP
ajlm-658	25	25	the	the	DET
ajlm-658	25	26	ncbi	ncbi	PROPN
ajlm-658	25	27	reference	reference	NOUN
ajlm-658	25	28	sequence	sequence	NOUN
ajlm-658	25	29	ng_009904	ng_009904	VERB
ajlm-658	25	30	.	.	PUNCT
ajlm-658	25	31	table	table	NOUN
ajlm-658	25	32	1	1	NUM
ajlm-658	25	33	:	:	PUNCT
ajlm-658	25	34	 	 	SPACE
ajlm-658	25	35	polymerase	polymerase	NOUN
ajlm-658	25	36	chain	chain	NOUN
ajlm-658	25	37	reaction	reaction	NOUN
ajlm-658	25	38	primer	primer	NOUN
ajlm-658	25	39	and	and	CCONJ
ajlm-658	25	40	probe	probe	NOUN
ajlm-658	25	41	sequences	sequence	NOUN
ajlm-658	25	42	used	use	VERB
ajlm-658	25	43	to	to	PART
ajlm-658	25	44	detect	detect	VERB
ajlm-658	25	45	the	the	DET
ajlm-658	25	46	jak2	jak2	PROPN
ajlm-658	25	47	v617f	v617f	PROPN
ajlm-658	25	48	mutation	mutation	NOUN
ajlm-658	25	49	(	(	PUNCT
ajlm-658	25	50	5′	5′	NUM
ajlm-658	25	51	→	→	SYM
ajlm-658	25	52	3	3	NUM
ajlm-658	25	53	)	)	PUNCT
ajlm-658	25	54	.	.	PUNCT
ajlm-658	26	1	locked	lock	VERB
ajlm-658	26	2	nucleic	nucleic	ADJ
ajlm-658	26	3	-	-	PUNCT
ajlm-658	26	4	acid	acid	ADJ
ajlm-658	26	5	,	,	PUNCT
ajlm-658	26	6	real	real	ADJ
ajlm-658	26	7	-	-	PUNCT
ajlm-658	26	8	time	time	NOUN
ajlm-658	26	9	polymerase	polymerase	NOUN
ajlm-658	26	10	chain	chain	NOUN
ajlm-658	26	11	reaction	reaction	NOUN
ajlm-658	26	12	assay	assay	VERB
ajlm-658	26	13	wild	wild	ADJ
ajlm-658	26	14	-	-	PUNCT
ajlm-658	26	15	type	type	NOUN
ajlm-658	26	16	and	and	CCONJ
ajlm-658	26	17	mutant	mutant	ADJ
ajlm-658	26	18	probes	probe	NOUN
ajlm-658	26	19	were	be	AUX
ajlm-658	26	20	designed	design	VERB
ajlm-658	26	21	to	to	PART
ajlm-658	26	22	be	be	AUX
ajlm-658	26	23	complementary	complementary	ADJ
ajlm-658	26	24	to	to	ADP
ajlm-658	26	25	the	the	DET
ajlm-658	26	26	wild	wild	ADJ
ajlm-658	26	27	-	-	PUNCT
ajlm-658	26	28	type	type	NOUN
ajlm-658	26	29	and	and	CCONJ
ajlm-658	26	30	mutant	mutant	ADJ
ajlm-658	26	31	nucleotide	nucleotide	NOUN
ajlm-658	26	32	sequences	sequence	NOUN
ajlm-658	26	33	(	(	PUNCT
ajlm-658	26	34	table	table	NOUN
ajlm-658	26	35	1	1	NUM
ajlm-658	26	36	)	)	PUNCT
ajlm-658	26	37	.	.	PUNCT
ajlm-658	27	1	the	the	DET
ajlm-658	27	2	real	real	ADJ
ajlm-658	27	3	-	-	PUNCT
ajlm-658	27	4	time	time	NOUN
ajlm-658	27	5	pcr	pcr	PROPN
ajlm-658	27	6	assay	assay	NOUN
ajlm-658	27	7	was	be	AUX
ajlm-658	27	8	performed	perform	VERB
ajlm-658	27	9	using	use	VERB
ajlm-658	27	10	the	the	DET
ajlm-658	27	11	cepheid	cepheid	PROPN
ajlm-658	27	12	smartcycler	smartcycler	PROPN
ajlm-658	27	13	ii	ii	PROPN
ajlm-658	27	14	platform	platform	NOUN
ajlm-658	27	15	(	(	PUNCT
ajlm-658	27	16	cepheid	cepheid	NOUN
ajlm-658	27	17	,	,	PUNCT
ajlm-658	27	18	maurens	mauren	NOUN
ajlm-658	27	19	-	-	PUNCT
ajlm-658	27	20	scopont	scopont	ADJ
ajlm-658	27	21	,	,	PUNCT
ajlm-658	27	22	france	france	PROPN
ajlm-658	27	23	)	)	PUNCT
ajlm-658	27	24	.	.	PUNCT
ajlm-658	28	1	the	the	DET
ajlm-658	28	2	reaction	reaction	NOUN
ajlm-658	28	3	mixture	mixture	NOUN
ajlm-658	28	4	contained	contain	VERB
ajlm-658	28	5	0.4	0.4	NUM
ajlm-658	28	6	µm	µm	ADP
ajlm-658	28	7	each	each	PRON
ajlm-658	28	8	of	of	ADP
ajlm-658	28	9	the	the	DET
ajlm-658	28	10	forward	forward	ADJ
ajlm-658	28	11	and	and	CCONJ
ajlm-658	28	12	reverse	reverse	ADJ
ajlm-658	28	13	primers	primer	NOUN
ajlm-658	28	14	,	,	PUNCT
ajlm-658	28	15	0.4	0.4	NUM
ajlm-658	28	16	µm	µm	NOUN
ajlm-658	28	17	of	of	ADP
ajlm-658	28	18	mutant	mutant	NOUN
ajlm-658	28	19	and	and	CCONJ
ajlm-658	28	20	0.05	0.05	NUM
ajlm-658	28	21	µm	µm	NOUN
ajlm-658	28	22	of	of	ADP
ajlm-658	28	23	wild	wild	ADJ
ajlm-658	28	24	-	-	PUNCT
ajlm-658	28	25	type	type	NOUN
ajlm-658	28	26	probes	probe	NOUN
ajlm-658	28	27	,	,	PUNCT
ajlm-658	28	28	2x	2x	NUM
ajlm-658	28	29	qiagen	qiagen	PROPN
ajlm-658	28	30	quantinovatm	quantinovatm	PROPN
ajlm-658	28	31	probe	probe	PROPN
ajlm-658	28	32	pcr	pcr	PROPN
ajlm-658	28	33	master	master	NOUN
ajlm-658	28	34	mix	mix	NOUN
ajlm-658	28	35	(	(	PUNCT
ajlm-658	28	36	qiagen	qiagen	NOUN
ajlm-658	28	37	,	,	PUNCT
ajlm-658	28	38	hilden	hilden	PROPN
ajlm-658	28	39	,	,	PUNCT
ajlm-658	28	40	germany	germany	PROPN
ajlm-658	28	41	)	)	PUNCT
ajlm-658	28	42	and	and	CCONJ
ajlm-658	28	43	<	<	X
ajlm-658	28	44	100	100	NUM
ajlm-658	28	45	ng	ng	PROPN
ajlm-658	28	46	template	template	NOUN
ajlm-658	28	47	gdna	gdna	ADV
ajlm-658	28	48	.	.	PUNCT
ajlm-658	29	1	in	in	ADP
ajlm-658	29	2	an	an	DET
ajlm-658	29	3	attempt	attempt	NOUN
ajlm-658	29	4	to	to	PART
ajlm-658	29	5	optimise	optimise	VERB
ajlm-658	29	6	the	the	DET
ajlm-658	29	7	assay	assay	NOUN
ajlm-658	29	8	’s	’s	PART
ajlm-658	29	9	performance	performance	NOUN
ajlm-658	29	10	,	,	PUNCT
ajlm-658	29	11	the	the	DET
ajlm-658	29	12	wild	wild	ADJ
ajlm-658	29	13	-	-	PUNCT
ajlm-658	29	14	type	type	NOUN
ajlm-658	29	15	probe	probe	NOUN
ajlm-658	29	16	concentration	concentration	NOUN
ajlm-658	29	17	was	be	AUX
ajlm-658	29	18	adjusted	adjust	VERB
ajlm-658	29	19	in	in	ADP
ajlm-658	29	20	a	a	DET
ajlm-658	29	21	separate	separate	ADJ
ajlm-658	29	22	experiment	experiment	NOUN
ajlm-658	29	23	by	by	ADP
ajlm-658	29	24	making	make	VERB
ajlm-658	29	25	a	a	DET
ajlm-658	29	26	serial	serial	ADJ
ajlm-658	29	27	dilution	dilution	NOUN
ajlm-658	29	28	of	of	ADP
ajlm-658	29	29	the	the	DET
ajlm-658	29	30	wild	wild	ADJ
ajlm-658	29	31	-	-	PUNCT
ajlm-658	29	32	type	type	NOUN
ajlm-658	29	33	probe	probe	NOUN
ajlm-658	29	34	in	in	ADP
ajlm-658	29	35	nuclease	nuclease	NOUN
ajlm-658	29	36	-	-	PUNCT
ajlm-658	29	37	free	free	ADJ
ajlm-658	29	38	water	water	NOUN
ajlm-658	29	39	and	and	CCONJ
ajlm-658	29	40	assessing	assess	VERB
ajlm-658	29	41	its	its	PRON
ajlm-658	29	42	performance	performance	NOUN
ajlm-658	29	43	.	.	PUNCT
ajlm-658	30	1	the	the	DET
ajlm-658	30	2	concentration	concentration	NOUN
ajlm-658	30	3	of	of	ADP
ajlm-658	30	4	the	the	DET
ajlm-658	30	5	wild	wild	ADJ
ajlm-658	30	6	-	-	PUNCT
ajlm-658	30	7	type	type	NOUN
ajlm-658	30	8	probe	probe	NOUN
ajlm-658	30	9	that	that	PRON
ajlm-658	30	10	gave	give	VERB
ajlm-658	30	11	optimal	optimal	ADJ
ajlm-658	30	12	results	result	NOUN
ajlm-658	30	13	was	be	AUX
ajlm-658	30	14	found	find	VERB
ajlm-658	30	15	to	to	PART
ajlm-658	30	16	be	be	AUX
ajlm-658	30	17	0.05	0.05	NUM
ajlm-658	30	18	µm	µm	NOUN
ajlm-658	30	19	.	.	PUNCT
ajlm-658	31	1	this	this	DET
ajlm-658	31	2	concentration	concentration	NOUN
ajlm-658	31	3	of	of	ADP
ajlm-658	31	4	wild	wild	ADJ
ajlm-658	31	5	-	-	PUNCT
ajlm-658	31	6	type	type	NOUN
ajlm-658	31	7	probe	probe	NOUN
ajlm-658	31	8	was	be	AUX
ajlm-658	31	9	used	use	VERB
ajlm-658	31	10	together	together	ADV
ajlm-658	31	11	with	with	ADP
ajlm-658	31	12	0.4	0.4	NUM
ajlm-658	31	13	µm	µm	ADP
ajlm-658	31	14	mutant	mutant	ADJ
ajlm-658	31	15	probe	probe	NOUN
ajlm-658	31	16	in	in	ADP
ajlm-658	31	17	the	the	DET
ajlm-658	31	18	pcr	pcr	PROPN
ajlm-658	31	19	assay	assay	VERB
ajlm-658	31	20	with	with	ADP
ajlm-658	31	21	satisfactory	satisfactory	ADJ
ajlm-658	31	22	results	result	NOUN
ajlm-658	31	23	.	.	PUNCT
ajlm-658	32	1	polymerase	polymerase	NOUN
ajlm-658	32	2	chain	chain	NOUN
ajlm-658	32	3	reaction	reaction	NOUN
ajlm-658	32	4	was	be	AUX
ajlm-658	32	5	performed	perform	VERB
ajlm-658	32	6	under	under	ADP
ajlm-658	32	7	the	the	DET
ajlm-658	32	8	following	following	ADJ
ajlm-658	32	9	conditions	condition	NOUN
ajlm-658	32	10	:	:	PUNCT
ajlm-658	32	11	initial	initial	ADJ
ajlm-658	32	12	denaturation	denaturation	NOUN
ajlm-658	32	13	at	at	ADP
ajlm-658	32	14	95	95	NUM
ajlm-658	32	15	°	°	ADP
ajlm-658	32	16	c	c	NOUN
ajlm-658	32	17	for	for	ADP
ajlm-658	32	18	two	two	NUM
ajlm-658	32	19	minutes	minute	NOUN
ajlm-658	32	20	,	,	PUNCT
ajlm-658	32	21	followed	follow	VERB
ajlm-658	32	22	by	by	ADP
ajlm-658	32	23	40	40	NUM
ajlm-658	32	24	cycles	cycle	NOUN
ajlm-658	32	25	of	of	ADP
ajlm-658	32	26	amplification	amplification	NOUN
ajlm-658	32	27	with	with	ADP
ajlm-658	32	28	denaturation	denaturation	NOUN
ajlm-658	32	29	at	at	ADP
ajlm-658	32	30	95	95	NUM
ajlm-658	32	31	°	°	ADP
ajlm-658	32	32	c	c	NOUN
ajlm-658	32	33	for	for	ADP
ajlm-658	32	34	10	10	NUM
ajlm-658	32	35	seconds	second	NOUN
ajlm-658	32	36	,	,	PUNCT
ajlm-658	32	37	then	then	ADV
ajlm-658	32	38	combined	combine	VERB
ajlm-658	32	39	annealing	annealing	NOUN
ajlm-658	32	40	and	and	CCONJ
ajlm-658	32	41	extension	extension	NOUN
ajlm-658	32	42	at	at	ADP
ajlm-658	32	43	60	60	NUM
ajlm-658	32	44	°	°	ADP
ajlm-658	32	45	c	c	NOUN
ajlm-658	32	46	for	for	ADP
ajlm-658	32	47	30	30	NUM
ajlm-658	32	48	seconds	second	NOUN
ajlm-658	32	49	.	.	PUNCT
ajlm-658	33	1	genomic	genomic	NOUN
ajlm-658	33	2	gblocks	gblock	NOUN
ajlm-658	33	3	®	®	NOUN
ajlm-658	33	4	gene	gene	NOUN
ajlm-658	33	5	fragments	fragment	NOUN
ajlm-658	33	6	(	(	PUNCT
ajlm-658	33	7	integrated	integrate	VERB
ajlm-658	33	8	dna	dna	PROPN
ajlm-658	33	9	technologies	technology	NOUN
ajlm-658	33	10	,	,	PUNCT
ajlm-658	33	11	coralville	coralville	PROPN
ajlm-658	33	12	,	,	PUNCT
ajlm-658	33	13	iowa	iowa	PROPN
ajlm-658	33	14	,	,	PUNCT
ajlm-658	33	15	united	united	PROPN
ajlm-658	33	16	states	states	PROPN
ajlm-658	33	17	)	)	PUNCT
ajlm-658	33	18	consisting	consist	VERB
ajlm-658	33	19	of	of	ADP
ajlm-658	33	20	mutant	mutant	ADJ
ajlm-658	33	21	and	and	CCONJ
ajlm-658	33	22	wild	wild	ADJ
ajlm-658	33	23	-	-	PUNCT
ajlm-658	33	24	type	type	NOUN
ajlm-658	33	25	sequences	sequence	NOUN
ajlm-658	33	26	each	each	PRON
ajlm-658	33	27	together	together	ADV
ajlm-658	33	28	with	with	ADP
ajlm-658	33	29	no	no	DET
ajlm-658	33	30	template	template	NOUN
ajlm-658	33	31	control	control	NOUN
ajlm-658	33	32	were	be	AUX
ajlm-658	33	33	included	include	VERB
ajlm-658	33	34	with	with	ADP
ajlm-658	33	35	each	each	DET
ajlm-658	33	36	run	run	NOUN
ajlm-658	33	37	.	.	PUNCT
ajlm-658	34	1	the	the	DET
ajlm-658	34	2	analytical	analytical	ADJ
ajlm-658	34	3	sensitivity	sensitivity	NOUN
ajlm-658	34	4	of	of	ADP
ajlm-658	34	5	the	the	DET
ajlm-658	34	6	locked	locked	ADJ
ajlm-658	34	7	nucleic	nucleic	ADJ
ajlm-658	34	8	-	-	PUNCT
ajlm-658	34	9	acid	acid	NOUN
ajlm-658	34	10	(	(	PUNCT
ajlm-658	34	11	lna	lna	PROPN
ajlm-658	34	12	)	)	PUNCT
ajlm-658	34	13	real	real	ADJ
ajlm-658	34	14	-	-	PUNCT
ajlm-658	34	15	time	time	NOUN
ajlm-658	34	16	pcr	pcr	PROPN
ajlm-658	34	17	assay	assay	NOUN
ajlm-658	34	18	was	be	AUX
ajlm-658	34	19	determined	determine	VERB
ajlm-658	34	20	using	use	VERB
ajlm-658	34	21	wild	wild	ADJ
ajlm-658	34	22	-	-	PUNCT
ajlm-658	34	23	type	type	NOUN
ajlm-658	34	24	dna	dna	NOUN
ajlm-658	34	25	mixed	mix	VERB
ajlm-658	34	26	with	with	ADP
ajlm-658	34	27	that	that	PRON
ajlm-658	34	28	of	of	ADP
ajlm-658	34	29	homozygous	homozygous	ADJ
ajlm-658	34	30	mutant	mutant	ADJ
ajlm-658	34	31	dna	dna	NOUN
ajlm-658	34	32	in	in	ADP
ajlm-658	34	33	various	various	ADJ
ajlm-658	34	34	concentrations	concentration	NOUN
ajlm-658	34	35	(	(	PUNCT
ajlm-658	34	36	100	100	NUM
ajlm-658	34	37	%	%	NOUN
ajlm-658	34	38	mutant	mutant	NOUN
ajlm-658	34	39	,	,	PUNCT
ajlm-658	34	40	50	50	NUM
ajlm-658	34	41	%	%	NOUN
ajlm-658	34	42	mutant	mutant	NOUN
ajlm-658	34	43	,	,	PUNCT
ajlm-658	34	44	20	20	NUM
ajlm-658	34	45	%	%	NOUN
ajlm-658	34	46	mutant	mutant	NOUN
ajlm-658	34	47	,	,	PUNCT
ajlm-658	34	48	10	10	NUM
ajlm-658	34	49	%	%	NOUN
ajlm-658	34	50	mutant	mutant	NOUN
ajlm-658	34	51	,	,	PUNCT
ajlm-658	34	52	5	5	NUM
ajlm-658	34	53	%	%	NOUN
ajlm-658	34	54	mutant	mutant	NOUN
ajlm-658	34	55	,	,	PUNCT
ajlm-658	34	56	2	2	NUM
ajlm-658	34	57	%	%	NOUN
ajlm-658	34	58	mutant	mutant	NOUN
ajlm-658	34	59	,	,	PUNCT
ajlm-658	34	60	1	1	NUM
ajlm-658	34	61	%	%	NOUN
ajlm-658	34	62	mutant	mutant	NOUN
ajlm-658	34	63	and	and	CCONJ
ajlm-658	34	64	0.1	0.1	NUM
ajlm-658	34	65	%	%	NOUN
ajlm-658	34	66	mutant	mutant	NOUN
ajlm-658	34	67	)	)	PUNCT
ajlm-658	34	68	.	.	PUNCT
ajlm-658	35	1	the	the	DET
ajlm-658	35	2	dilutions	dilution	NOUN
ajlm-658	35	3	were	be	AUX
ajlm-658	35	4	prepared	prepare	VERB
ajlm-658	35	5	fresh	fresh	ADJ
ajlm-658	35	6	and	and	CCONJ
ajlm-658	35	7	subjected	subject	VERB
ajlm-658	35	8	to	to	ADP
ajlm-658	35	9	pcr	pcr	NOUN
ajlm-658	35	10	in	in	ADP
ajlm-658	35	11	three	three	NUM
ajlm-658	35	12	replicate	replicate	ADJ
ajlm-658	35	13	experiments	experiment	NOUN
ajlm-658	35	14	to	to	PART
ajlm-658	35	15	ensure	ensure	VERB
ajlm-658	35	16	reproducibility	reproducibility	NOUN
ajlm-658	35	17	of	of	ADP
ajlm-658	35	18	the	the	DET
ajlm-658	35	19	assay	assay	NOUN
ajlm-658	35	20	.	.	PUNCT
ajlm-658	36	1	the	the	DET
ajlm-658	36	2	limit	limit	NOUN
ajlm-658	36	3	of	of	ADP
ajlm-658	36	4	detection	detection	NOUN
ajlm-658	36	5	was	be	AUX
ajlm-658	36	6	defined	define	VERB
ajlm-658	36	7	as	as	ADP
ajlm-658	36	8	the	the	DET
ajlm-658	36	9	lowest	low	ADJ
ajlm-658	36	10	dilution	dilution	NOUN
ajlm-658	36	11	of	of	ADP
ajlm-658	36	12	mutant	mutant	ADJ
ajlm-658	36	13	dna	dna	NOUN
ajlm-658	36	14	in	in	ADP
ajlm-658	36	15	which	which	PRON
ajlm-658	36	16	all	all	DET
ajlm-658	36	17	three	three	NUM
ajlm-658	36	18	replicates	replicate	NOUN
ajlm-658	36	19	resulted	result	VERB
ajlm-658	36	20	in	in	ADP
ajlm-658	36	21	positive	positive	ADJ
ajlm-658	36	22	amplification.14	amplification.14	PROPN
ajlm-658	36	23	data	datum	NOUN
ajlm-658	36	24	and	and	CCONJ
ajlm-658	36	25	statistical	statistical	ADJ
ajlm-658	36	26	analysis	analysis	NOUN
ajlm-658	36	27	the	the	DET
ajlm-658	36	28	real	real	ADJ
ajlm-658	36	29	-	-	PUNCT
ajlm-658	36	30	time	time	NOUN
ajlm-658	36	31	pcr	pcr	PROPN
ajlm-658	36	32	assay	assay	NOUN
ajlm-658	36	33	results	result	NOUN
ajlm-658	36	34	were	be	AUX
ajlm-658	36	35	compared	compare	VERB
ajlm-658	36	36	to	to	ADP
ajlm-658	36	37	the	the	DET
ajlm-658	36	38	sequencing	sequence	VERB
ajlm-658	36	39	assay	assay	NOUN
ajlm-658	36	40	results	result	NOUN
ajlm-658	36	41	to	to	PART
ajlm-658	36	42	assess	assess	VERB
ajlm-658	36	43	the	the	DET
ajlm-658	36	44	assay	assay	NOUN
ajlm-658	36	45	’s	’s	PART
ajlm-658	36	46	usefulness	usefulness	NOUN
ajlm-658	36	47	for	for	ADP
ajlm-658	36	48	allelic	allelic	ADJ
ajlm-658	36	49	discrimination	discrimination	NOUN
ajlm-658	36	50	of	of	ADP
ajlm-658	36	51	the	the	DET
ajlm-658	36	52	jak2	jak2	PROPN
ajlm-658	36	53	v617f	v617f	PROPN
ajlm-658	36	54	mutation	mutation	NOUN
ajlm-658	36	55	.	.	PUNCT
ajlm-658	37	1	the	the	DET
ajlm-658	37	2	results	result	NOUN
ajlm-658	37	3	were	be	AUX
ajlm-658	37	4	used	use	VERB
ajlm-658	37	5	to	to	PART
ajlm-658	37	6	assess	assess	VERB
ajlm-658	37	7	the	the	DET
ajlm-658	37	8	agreement	agreement	NOUN
ajlm-658	37	9	between	between	ADP
ajlm-658	37	10	the	the	DET
ajlm-658	37	11	two	two	NUM
ajlm-658	37	12	assays	assay	NOUN
ajlm-658	37	13	using	use	VERB
ajlm-658	37	14	cohen	cohen	PROPN
ajlm-658	37	15	’s	’s	PART
ajlm-658	37	16	kappa	kappa	PROPN
ajlm-658	37	17	for	for	ADP
ajlm-658	37	18	inter	inter	ADJ
ajlm-658	37	19	-	-	ADJ
ajlm-658	37	20	rater	rater	ADJ
ajlm-658	37	21	agreement	agreement	NOUN
ajlm-658	37	22	to	to	PART
ajlm-658	37	23	assess	assess	VERB
ajlm-658	37	24	the	the	DET
ajlm-658	37	25	agreement	agreement	NOUN
ajlm-658	37	26	between	between	ADP
ajlm-658	37	27	methods	method	NOUN
ajlm-658	37	28	using	use	VERB
ajlm-658	37	29	interpretation	interpretation	NOUN
ajlm-658	37	30	cut	cut	NOUN
ajlm-658	37	31	-	-	PUNCT
ajlm-658	37	32	offs	off	NOUN
ajlm-658	37	33	:	:	PUNCT
ajlm-658	37	34	κ	κ	X
ajlm-658	37	35	>	>	X
ajlm-658	37	36	0.75	0.75	NUM
ajlm-658	37	37	excellent	excellent	ADJ
ajlm-658	37	38	agreement	agreement	NOUN
ajlm-658	37	39	,	,	PUNCT
ajlm-658	37	40	κ	κ	PROPN
ajlm-658	37	41	=	=	SYM
ajlm-658	37	42	0.4–0.75	0.4–0.75	PROPN
ajlm-658	37	43	good	good	ADJ
ajlm-658	37	44	agreement	agreement	NOUN
ajlm-658	37	45	and	and	CCONJ
ajlm-658	37	46	κ	κ	X
ajlm-658	37	47	<	<	X
ajlm-658	37	48	0.4	0.4	NUM
ajlm-658	37	49	poor	poor	ADJ
ajlm-658	37	50	agreement.15	agreement.15	NOUN
ajlm-658	37	51	results	result	NOUN
ajlm-658	37	52	both	both	CCONJ
ajlm-658	37	53	sequencing	sequence	VERB
ajlm-658	37	54	and	and	CCONJ
ajlm-658	37	55	the	the	DET
ajlm-658	37	56	lna	lna	PROPN
ajlm-658	37	57	real	real	ADJ
ajlm-658	37	58	-	-	PUNCT
ajlm-658	37	59	time	time	NOUN
ajlm-658	37	60	pcr	pcr	PROPN
ajlm-658	37	61	assay	assay	NOUN
ajlm-658	37	62	detected	detect	VERB
ajlm-658	37	63	the	the	DET
ajlm-658	37	64	mutation	mutation	NOUN
ajlm-658	37	65	in	in	ADP
ajlm-658	37	66	the	the	DET
ajlm-658	37	67	same	same	ADJ
ajlm-658	37	68	24	24	NUM
ajlm-658	37	69	samples	sample	NOUN
ajlm-658	37	70	(	(	PUNCT
ajlm-658	37	71	40	40	NUM
ajlm-658	37	72	%	%	NOUN
ajlm-658	37	73	)	)	PUNCT
ajlm-658	37	74	and	and	CCONJ
ajlm-658	37	75	with	with	ADP
ajlm-658	37	76	κ	κ	NOUN
ajlm-658	37	77	=	=	SYM
ajlm-658	37	78	1	1	NUM
ajlm-658	37	79	(	(	PUNCT
ajlm-658	37	80	i.e.	i.e.	X
ajlm-658	37	81	perfect	perfect	ADJ
ajlm-658	37	82	agreement	agreement	NOUN
ajlm-658	37	83	)	)	PUNCT
ajlm-658	37	84	.	.	PUNCT
ajlm-658	38	1	during	during	ADP
ajlm-658	38	2	the	the	DET
ajlm-658	38	3	experiment	experiment	NOUN
ajlm-658	38	4	,	,	PUNCT
ajlm-658	38	5	it	it	PRON
ajlm-658	38	6	was	be	AUX
ajlm-658	38	7	noted	note	VERB
ajlm-658	38	8	that	that	SCONJ
ajlm-658	38	9	the	the	DET
ajlm-658	38	10	standard	standard	ADJ
ajlm-658	38	11	1:1	1:1	NUM
ajlm-658	38	12	wild	wild	ADJ
ajlm-658	38	13	-	-	PUNCT
ajlm-658	38	14	type	type	NOUN
ajlm-658	38	15	to	to	PART
ajlm-658	38	16	mutant	mutant	ADJ
ajlm-658	38	17	probe	probe	NOUN
ajlm-658	38	18	ratio	ratio	NOUN
ajlm-658	38	19	did	do	AUX
ajlm-658	38	20	not	not	PART
ajlm-658	38	21	provide	provide	VERB
ajlm-658	38	22	optimal	optimal	ADJ
ajlm-658	38	23	results	result	NOUN
ajlm-658	38	24	.	.	PUNCT
ajlm-658	39	1	the	the	DET
ajlm-658	39	2	ratio	ratio	NOUN
ajlm-658	39	3	of	of	ADP
ajlm-658	39	4	wild	wild	ADJ
ajlm-658	39	5	-	-	PUNCT
ajlm-658	39	6	type	type	NOUN
ajlm-658	39	7	to	to	AUX
ajlm-658	39	8	mutant	mutant	ADJ
ajlm-658	39	9	probe	probe	NOUN
ajlm-658	39	10	that	that	PRON
ajlm-658	39	11	provided	provide	VERB
ajlm-658	39	12	optimal	optimal	ADJ
ajlm-658	39	13	results	result	NOUN
ajlm-658	39	14	was	be	AUX
ajlm-658	39	15	1:8	1:8	NUM
ajlm-658	39	16	(	(	PUNCT
ajlm-658	39	17	figures	figure	NOUN
ajlm-658	39	18	1	1	NUM
ajlm-658	39	19	and	and	CCONJ
ajlm-658	39	20	2	2	NUM
ajlm-658	39	21	show	show	VERB
ajlm-658	39	22	how	how	SCONJ
ajlm-658	39	23	a	a	DET
ajlm-658	39	24	heterozygous	heterozygous	ADJ
ajlm-658	39	25	sample	sample	NOUN
ajlm-658	39	26	amplifies	amplify	VERB
ajlm-658	39	27	when	when	SCONJ
ajlm-658	39	28	the	the	DET
ajlm-658	39	29	1:1	1:1	NUM
ajlm-658	39	30	and	and	CCONJ
ajlm-658	39	31	1:8	1:8	NUM
ajlm-658	39	32	ratios	ratio	NOUN
ajlm-658	39	33	of	of	ADP
ajlm-658	39	34	mutant	mutant	NOUN
ajlm-658	39	35	to	to	ADP
ajlm-658	39	36	wild	wild	ADJ
ajlm-658	39	37	-	-	PUNCT
ajlm-658	39	38	type	type	NOUN
ajlm-658	39	39	probes	probe	NOUN
ajlm-658	39	40	were	be	AUX
ajlm-658	39	41	used	use	VERB
ajlm-658	39	42	)	)	PUNCT
ajlm-658	39	43	.	.	PUNCT
ajlm-658	40	1	the	the	DET
ajlm-658	40	2	limit	limit	NOUN
ajlm-658	40	3	of	of	ADP
ajlm-658	40	4	detection	detection	NOUN
ajlm-658	40	5	of	of	ADP
ajlm-658	40	6	this	this	DET
ajlm-658	40	7	assay	assay	NOUN
ajlm-658	40	8	is	be	AUX
ajlm-658	40	9	0.1	0.1	NUM
ajlm-658	40	10	%	%	NOUN
ajlm-658	40	11	in	in	ADP
ajlm-658	40	12	a	a	DET
ajlm-658	40	13	wild	wild	ADJ
ajlm-658	40	14	-	-	PUNCT
ajlm-658	40	15	type	type	NOUN
ajlm-658	40	16	dna	dna	NOUN
ajlm-658	40	17	background	background	NOUN
ajlm-658	40	18	(	(	PUNCT
ajlm-658	40	19	figure	figure	NOUN
ajlm-658	40	20	3	3	NUM
ajlm-658	40	21	)	)	PUNCT
ajlm-658	40	22	.	.	PUNCT
ajlm-658	41	1	figure	figure	VERB
ajlm-658	41	2	1	1	NUM
ajlm-658	41	3	:	:	PUNCT
ajlm-658	41	4	an	an	DET
ajlm-658	41	5	amplification	amplification	NOUN
ajlm-658	41	6	curve	curve	NOUN
ajlm-658	41	7	showing	show	VERB
ajlm-658	41	8	results	result	NOUN
ajlm-658	41	9	with	with	ADP
ajlm-658	41	10	0.4	0.4	NUM
ajlm-658	41	11	µm	µm	ADP
ajlm-658	41	12	wild	wild	ADJ
ajlm-658	41	13	-	-	PUNCT
ajlm-658	41	14	type	type	NOUN
ajlm-658	41	15	and	and	CCONJ
ajlm-658	41	16	0.4	0.4	NUM
ajlm-658	41	17	µm	µm	ADP
ajlm-658	41	18	mutant	mutant	ADJ
ajlm-658	41	19	probes	probe	NOUN
ajlm-658	41	20	.	.	PUNCT
ajlm-658	42	1	mutated	mutate	VERB
ajlm-658	42	2	allele	allele	PROPN
ajlm-658	42	3	curve	curve	NOUN
ajlm-658	42	4	with	with	ADP
ajlm-658	42	5	no	no	DET
ajlm-658	42	6	markers	marker	NOUN
ajlm-658	42	7	,	,	PUNCT
ajlm-658	42	8	wild	wild	ADJ
ajlm-658	42	9	-	-	PUNCT
ajlm-658	42	10	type	type	NOUN
ajlm-658	42	11	allele	allele	NOUN
ajlm-658	42	12	curve	curve	NOUN
ajlm-658	42	13	with	with	ADP
ajlm-658	42	14	crosses	crosse	NOUN
ajlm-658	42	15	.	.	PUNCT
ajlm-658	43	1	figure	figure	NOUN
ajlm-658	43	2	2	2	NUM
ajlm-658	43	3	:	:	PUNCT
ajlm-658	43	4	an	an	DET
ajlm-658	43	5	amplification	amplification	NOUN
ajlm-658	43	6	curve	curve	NOUN
ajlm-658	43	7	showing	show	VERB
ajlm-658	43	8	results	result	NOUN
ajlm-658	43	9	with	with	ADP
ajlm-658	43	10	0.05	0.05	NUM
ajlm-658	43	11	µm	µm	ADP
ajlm-658	43	12	wild	wild	ADJ
ajlm-658	43	13	-	-	PUNCT
ajlm-658	43	14	type	type	NOUN
ajlm-658	43	15	and	and	CCONJ
ajlm-658	43	16	0.4	0.4	NUM
ajlm-658	43	17	µm	µm	ADP
ajlm-658	43	18	mutant	mutant	ADJ
ajlm-658	43	19	probes	probe	NOUN
ajlm-658	43	20	.	.	PUNCT
ajlm-658	44	1	mutated	mutate	VERB
ajlm-658	44	2	allele	allele	PROPN
ajlm-658	44	3	curve	curve	NOUN
ajlm-658	44	4	with	with	ADP
ajlm-658	44	5	no	no	DET
ajlm-658	44	6	markers	marker	NOUN
ajlm-658	44	7	,	,	PUNCT
ajlm-658	44	8	wild	wild	ADJ
ajlm-658	44	9	-	-	PUNCT
ajlm-658	44	10	type	type	NOUN
ajlm-658	44	11	allele	allele	NOUN
ajlm-658	44	12	curve	curve	NOUN
ajlm-658	44	13	with	with	ADP
ajlm-658	44	14	crosses	crosse	NOUN
ajlm-658	44	15	.	.	PUNCT
ajlm-658	45	1	figure	figure	VERB
ajlm-658	45	2	3	3	NUM
ajlm-658	45	3	:	:	PUNCT
ajlm-658	45	4	amplification	amplification	NOUN
ajlm-658	45	5	curves	curve	NOUN
ajlm-658	45	6	showing	show	VERB
ajlm-658	45	7	the	the	DET
ajlm-658	45	8	analytical	analytical	ADJ
ajlm-658	45	9	sensitivity	sensitivity	NOUN
ajlm-658	45	10	of	of	ADP
ajlm-658	45	11	one	one	NUM
ajlm-658	45	12	sample	sample	NOUN
ajlm-658	45	13	.	.	PUNCT
ajlm-658	46	1	green	green	ADJ
ajlm-658	46	2	:	:	PUNCT
ajlm-658	46	3	100	100	NUM
ajlm-658	46	4	%	%	NOUN
ajlm-658	46	5	homozygous	homozygous	ADJ
ajlm-658	46	6	mutant	mutant	NOUN
ajlm-658	46	7	dna	dna	NOUN
ajlm-658	46	8	;	;	PUNCT
ajlm-658	46	9	red	red	ADJ
ajlm-658	46	10	:	:	PUNCT
ajlm-658	46	11	20	20	NUM
ajlm-658	46	12	%	%	NOUN
ajlm-658	46	13	mutant	mutant	NOUN
ajlm-658	46	14	with	with	ADP
ajlm-658	46	15	80	80	NUM
ajlm-658	46	16	%	%	NOUN
ajlm-658	46	17	wild	wild	ADJ
ajlm-658	46	18	-	-	PUNCT
ajlm-658	46	19	type	type	NOUN
ajlm-658	46	20	dna	dna	NOUN
ajlm-658	46	21	;	;	PUNCT
ajlm-658	46	22	blue	blue	ADJ
ajlm-658	46	23	:	:	PUNCT
ajlm-658	46	24	0.1	0.1	NUM
ajlm-658	46	25	%	%	NOUN
ajlm-658	46	26	mutant	mutant	ADJ
ajlm-658	46	27	dna	dna	NOUN
ajlm-658	46	28	in	in	ADP
ajlm-658	46	29	99.9	99.9	NUM
ajlm-658	46	30	%	%	NOUN
ajlm-658	46	31	of	of	ADP
ajlm-658	46	32	wild	wild	ADJ
ajlm-658	46	33	-	-	PUNCT
ajlm-658	46	34	type	type	NOUN
ajlm-658	46	35	dna	dna	NOUN
ajlm-658	46	36	.	.	PUNCT
ajlm-658	47	1	discussion	discussion	NOUN
ajlm-658	47	2	in	in	ADP
ajlm-658	47	3	this	this	DET
ajlm-658	47	4	study	study	NOUN
ajlm-658	47	5	,	,	PUNCT
ajlm-658	47	6	an	an	DET
ajlm-658	47	7	lna	lna	PROPN
ajlm-658	47	8	probe	probe	NOUN
ajlm-658	47	9	-	-	PUNCT
ajlm-658	47	10	based	base	VERB
ajlm-658	47	11	,	,	PUNCT
ajlm-658	47	12	real	real	ADJ
ajlm-658	47	13	-	-	PUNCT
ajlm-658	47	14	time	time	NOUN
ajlm-658	47	15	pcr	pcr	PROPN
ajlm-658	47	16	assay	assay	NOUN
ajlm-658	47	17	was	be	AUX
ajlm-658	47	18	developed	develop	VERB
ajlm-658	47	19	and	and	CCONJ
ajlm-658	47	20	evaluated	evaluate	VERB
ajlm-658	47	21	for	for	ADP
ajlm-658	47	22	its	its	PRON
ajlm-658	47	23	ability	ability	NOUN
ajlm-658	47	24	to	to	PART
ajlm-658	47	25	detect	detect	VERB
ajlm-658	47	26	the	the	DET
ajlm-658	47	27	jak2	jak2	PROPN
ajlm-658	47	28	v617f	v617f	PROPN
ajlm-658	47	29	mutation	mutation	NOUN
ajlm-658	47	30	.	.	PUNCT
ajlm-658	48	1	an	an	DET
ajlm-658	48	2	lna	lna	PROPN
ajlm-658	48	3	is	be	AUX
ajlm-658	48	4	an	an	DET
ajlm-658	48	5	analogue	analogue	NOUN
ajlm-658	48	6	of	of	ADP
ajlm-658	48	7	nucleotides	nucleotide	NOUN
ajlm-658	48	8	that	that	PRON
ajlm-658	48	9	contains	contain	VERB
ajlm-658	48	10	an	an	DET
ajlm-658	48	11	internal	internal	ADJ
ajlm-658	48	12	2′-o	2′-o	NUM
ajlm-658	48	13	,	,	PUNCT
ajlm-658	48	14	4′-c	4′-c	NUM
ajlm-658	48	15	methylene	methylene	NOUN
ajlm-658	48	16	bridge	bridge	NOUN
ajlm-658	48	17	,	,	PUNCT
ajlm-658	48	18	which	which	PRON
ajlm-658	48	19	locks	lock	VERB
ajlm-658	48	20	the	the	DET
ajlm-658	48	21	ribose	ribose	NOUN
ajlm-658	48	22	ring	ring	NOUN
ajlm-658	48	23	into	into	ADP
ajlm-658	48	24	a	a	DET
ajlm-658	48	25	c3′-endo	c3′-endo	PROPN
ajlm-658	48	26	conformation.16	conformation.16	ADJ
ajlm-658	48	27	introduction	introduction	NOUN
ajlm-658	48	28	of	of	ADP
ajlm-658	48	29	lnas	lna	NOUN
ajlm-658	48	30	into	into	ADP
ajlm-658	48	31	probes	probe	NOUN
ajlm-658	48	32	increases	increase	VERB
ajlm-658	48	33	the	the	DET
ajlm-658	48	34	thermal	thermal	ADJ
ajlm-658	48	35	stability	stability	NOUN
ajlm-658	48	36	of	of	ADP
ajlm-658	48	37	the	the	DET
ajlm-658	48	38	probe	probe	NOUN
ajlm-658	48	39	by	by	ADP
ajlm-658	48	40	+	+	NOUN
ajlm-658	48	41	3	3	NUM
ajlm-658	48	42	°	°	NOUN
ajlm-658	48	43	c	c	NOUN
ajlm-658	48	44	to	to	ADP
ajlm-658	48	45	+	+	NOUN
ajlm-658	48	46	8	8	NUM
ajlm-658	48	47	°	°	NOUN
ajlm-658	48	48	c	c	NOUN
ajlm-658	48	49	per	per	ADP
ajlm-658	48	50	modification	modification	NOUN
ajlm-658	48	51	and	and	CCONJ
ajlm-658	48	52	allows	allow	VERB
ajlm-658	48	53	binding	bind	VERB
ajlm-658	48	54	to	to	ADP
ajlm-658	48	55	complementary	complementary	ADJ
ajlm-658	48	56	target	target	NOUN
ajlm-658	48	57	sequences	sequence	NOUN
ajlm-658	48	58	with	with	ADP
ajlm-658	48	59	high	high	ADJ
ajlm-658	48	60	affinity.16,17,18	affinity.16,17,18	X
ajlm-658	48	61	in	in	ADP
ajlm-658	48	62	this	this	DET
ajlm-658	48	63	study	study	NOUN
ajlm-658	48	64	,	,	PUNCT
ajlm-658	48	65	the	the	DET
ajlm-658	48	66	wild	wild	ADJ
ajlm-658	48	67	-	-	PUNCT
ajlm-658	48	68	type	type	NOUN
ajlm-658	48	69	probe	probe	NOUN
ajlm-658	48	70	contained	contain	VERB
ajlm-658	48	71	six	six	NUM
ajlm-658	48	72	lnas	lna	NOUN
ajlm-658	48	73	and	and	CCONJ
ajlm-658	48	74	the	the	DET
ajlm-658	48	75	mutant	mutant	NOUN
ajlm-658	48	76	probe	probe	VERB
ajlm-658	48	77	seven	seven	NUM
ajlm-658	48	78	lnas	lna	NOUN
ajlm-658	48	79	.	.	PUNCT
ajlm-658	49	1	increasing	increase	VERB
ajlm-658	49	2	the	the	DET
ajlm-658	49	3	number	number	NOUN
ajlm-658	49	4	of	of	ADP
ajlm-658	49	5	lnas	lna	NOUN
ajlm-658	49	6	in	in	ADP
ajlm-658	49	7	the	the	DET
ajlm-658	49	8	mutant	mutant	ADJ
ajlm-658	49	9	probe	probe	NOUN
ajlm-658	49	10	was	be	AUX
ajlm-658	49	11	necessary	necessary	ADJ
ajlm-658	49	12	to	to	PART
ajlm-658	49	13	increase	increase	VERB
ajlm-658	49	14	the	the	DET
ajlm-658	49	15	melting	melting	NOUN
ajlm-658	49	16	temperature	temperature	NOUN
ajlm-658	49	17	(	(	PUNCT
ajlm-658	49	18	tm	tm	NOUN
ajlm-658	49	19	)	)	PUNCT
ajlm-658	49	20	of	of	ADP
ajlm-658	49	21	the	the	DET
ajlm-658	49	22	probe	probe	NOUN
ajlm-658	49	23	to	to	ADP
ajlm-658	49	24	greater	great	ADJ
ajlm-658	49	25	than	than	ADP
ajlm-658	49	26	that	that	PRON
ajlm-658	49	27	of	of	ADP
ajlm-658	49	28	the	the	DET
ajlm-658	49	29	primers	primer	NOUN
ajlm-658	49	30	and	and	CCONJ
ajlm-658	49	31	thus	thus	ADV
ajlm-658	49	32	increase	increase	VERB
ajlm-658	49	33	the	the	DET
ajlm-658	49	34	specificity	specificity	NOUN
ajlm-658	49	35	of	of	ADP
ajlm-658	49	36	the	the	DET
ajlm-658	49	37	probe	probe	NOUN
ajlm-658	49	38	.	.	PUNCT
ajlm-658	50	1	the	the	DET
ajlm-658	50	2	wild	wild	ADJ
ajlm-658	50	3	-	-	PUNCT
ajlm-658	50	4	type	type	NOUN
ajlm-658	50	5	probe	probe	NOUN
ajlm-658	50	6	binds	bind	VERB
ajlm-658	50	7	with	with	ADP
ajlm-658	50	8	high	high	ADJ
ajlm-658	50	9	affinity	affinity	NOUN
ajlm-658	50	10	,	,	PUNCT
ajlm-658	50	11	thus	thus	ADV
ajlm-658	50	12	reducing	reduce	VERB
ajlm-658	50	13	the	the	DET
ajlm-658	50	14	intensity	intensity	NOUN
ajlm-658	50	15	of	of	ADP
ajlm-658	50	16	the	the	DET
ajlm-658	50	17	mutant	mutant	ADJ
ajlm-658	50	18	probe	probe	NOUN
ajlm-658	50	19	as	as	SCONJ
ajlm-658	50	20	it	it	PRON
ajlm-658	50	21	is	be	AUX
ajlm-658	50	22	competing	compete	VERB
ajlm-658	50	23	with	with	ADP
ajlm-658	50	24	the	the	DET
ajlm-658	50	25	mutant	mutant	ADJ
ajlm-658	50	26	probe	probe	NOUN
ajlm-658	50	27	in	in	ADP
ajlm-658	50	28	this	this	DET
ajlm-658	50	29	multiplexed	multiplexed	ADJ
ajlm-658	50	30	assay	assay	NOUN
ajlm-658	50	31	.	.	PUNCT
ajlm-658	51	1	both	both	PRON
ajlm-658	51	2	sequencing	sequence	VERB
ajlm-658	51	3	and	and	CCONJ
ajlm-658	51	4	lna	lna	PROPN
ajlm-658	51	5	real	real	ADJ
ajlm-658	51	6	-	-	PUNCT
ajlm-658	51	7	time	time	NOUN
ajlm-658	51	8	pcr	pcr	ADJ
ajlm-658	51	9	assays	assay	NOUN
ajlm-658	51	10	detected	detect	VERB
ajlm-658	51	11	the	the	DET
ajlm-658	51	12	mutation	mutation	NOUN
ajlm-658	51	13	in	in	ADP
ajlm-658	51	14	40	40	NUM
ajlm-658	51	15	%	%	NOUN
ajlm-658	51	16	of	of	ADP
ajlm-658	51	17	the	the	DET
ajlm-658	51	18	samples	sample	NOUN
ajlm-658	51	19	.	.	PUNCT
ajlm-658	52	1	the	the	DET
ajlm-658	52	2	agreement	agreement	NOUN
ajlm-658	52	3	between	between	ADP
ajlm-658	52	4	the	the	DET
ajlm-658	52	5	real	real	ADJ
ajlm-658	52	6	-	-	PUNCT
ajlm-658	52	7	time	time	NOUN
ajlm-658	52	8	pcr	pcr	NOUN
ajlm-658	52	9	assay	assay	NOUN
ajlm-658	52	10	and	and	CCONJ
ajlm-658	52	11	sequencing	sequencing	NOUN
ajlm-658	52	12	was	be	AUX
ajlm-658	52	13	100	100	NUM
ajlm-658	52	14	%	%	NOUN
ajlm-658	52	15	.	.	PUNCT
ajlm-658	53	1	consistent	consistent	ADJ
ajlm-658	53	2	positive	positive	ADJ
ajlm-658	53	3	amplification	amplification	NOUN
ajlm-658	53	4	was	be	AUX
ajlm-658	53	5	obtained	obtain	VERB
ajlm-658	53	6	down	down	ADP
ajlm-658	53	7	to	to	ADP
ajlm-658	53	8	the	the	DET
ajlm-658	53	9	0.1	0.1	NUM
ajlm-658	53	10	%	%	NOUN
ajlm-658	53	11	dilution	dilution	NOUN
ajlm-658	53	12	(	(	PUNCT
ajlm-658	53	13	figure	figure	NOUN
ajlm-658	53	14	3	3	NUM
ajlm-658	53	15	)	)	PUNCT
ajlm-658	53	16	.	.	PUNCT
ajlm-658	54	1	the	the	DET
ajlm-658	54	2	limit	limit	NOUN
ajlm-658	54	3	of	of	ADP
ajlm-658	54	4	detection	detection	NOUN
ajlm-658	54	5	is	be	AUX
ajlm-658	54	6	determined	determine	VERB
ajlm-658	54	7	experimentally	experimentally	ADV
ajlm-658	54	8	by	by	ADP
ajlm-658	54	9	preparing	prepare	VERB
ajlm-658	54	10	serial	serial	ADJ
ajlm-658	54	11	dilutions	dilution	NOUN
ajlm-658	54	12	of	of	ADP
ajlm-658	54	13	mutant	mutant	ADJ
ajlm-658	54	14	dna	dna	NOUN
ajlm-658	54	15	in	in	ADP
ajlm-658	54	16	a	a	DET
ajlm-658	54	17	wild	wild	ADJ
ajlm-658	54	18	-	-	PUNCT
ajlm-658	54	19	type	type	NOUN
ajlm-658	54	20	dna	dna	NOUN
ajlm-658	54	21	background	background	NOUN
ajlm-658	54	22	and	and	CCONJ
ajlm-658	54	23	analysing	analyse	VERB
ajlm-658	54	24	each	each	DET
ajlm-658	54	25	dilution	dilution	NOUN
ajlm-658	54	26	point	point	NOUN
ajlm-658	54	27	in	in	ADP
ajlm-658	54	28	a	a	DET
ajlm-658	54	29	six	six	NUM
ajlm-658	54	30	-	-	ADJ
ajlm-658	54	31	fold	fold	ADJ
ajlm-658	54	32	run.14	run.14	NOUN
ajlm-658	54	33	the	the	DET
ajlm-658	54	34	last	last	ADJ
ajlm-658	54	35	dilution	dilution	NOUN
ajlm-658	54	36	where	where	SCONJ
ajlm-658	54	37	all	all	DET
ajlm-658	54	38	six	six	NUM
ajlm-658	54	39	replicates	replicate	NOUN
ajlm-658	54	40	give	give	VERB
ajlm-658	54	41	a	a	DET
ajlm-658	54	42	positive	positive	ADJ
ajlm-658	54	43	and	and	CCONJ
ajlm-658	54	44	specific	specific	ADJ
ajlm-658	54	45	amplification	amplification	NOUN
ajlm-658	54	46	is	be	AUX
ajlm-658	54	47	considered	consider	VERB
ajlm-658	54	48	to	to	PART
ajlm-658	54	49	be	be	AUX
ajlm-658	54	50	the	the	DET
ajlm-658	54	51	limit	limit	NOUN
ajlm-658	54	52	of	of	ADP
ajlm-658	54	53	detection.14	detection.14	PROPN
ajlm-658	54	54	the	the	DET
ajlm-658	54	55	acceptable	acceptable	ADJ
ajlm-658	54	56	sensitivity	sensitivity	NOUN
ajlm-658	54	57	of	of	ADP
ajlm-658	54	58	a	a	DET
ajlm-658	54	59	qualitative	qualitative	ADJ
ajlm-658	54	60	test	test	NOUN
ajlm-658	54	61	should	should	AUX
ajlm-658	54	62	be	be	AUX
ajlm-658	54	63	equal	equal	ADJ
ajlm-658	54	64	to	to	ADP
ajlm-658	54	65	or	or	CCONJ
ajlm-658	54	66	below	below	ADP
ajlm-658	54	67	20%.14	20%.14	NUM
ajlm-658	54	68	however	however	ADV
ajlm-658	54	69	,	,	PUNCT
ajlm-658	54	70	it	it	PRON
ajlm-658	54	71	is	be	AUX
ajlm-658	54	72	recommended	recommend	VERB
ajlm-658	54	73	that	that	SCONJ
ajlm-658	54	74	assays	assay	NOUN
ajlm-658	54	75	for	for	ADP
ajlm-658	54	76	the	the	DET
ajlm-658	54	77	detection	detection	NOUN
ajlm-658	54	78	of	of	ADP
ajlm-658	54	79	the	the	DET
ajlm-658	54	80	jak2	jak2	PROPN
ajlm-658	54	81	v617f	v617f	PROPN
ajlm-658	54	82	mutation	mutation	NOUN
ajlm-658	54	83	in	in	ADP
ajlm-658	54	84	a	a	DET
ajlm-658	54	85	clinical	clinical	ADJ
ajlm-658	54	86	setting	setting	NOUN
ajlm-658	54	87	should	should	AUX
ajlm-658	54	88	have	have	VERB
ajlm-658	54	89	an	an	DET
ajlm-658	54	90	analytical	analytical	ADJ
ajlm-658	54	91	sensitivity	sensitivity	NOUN
ajlm-658	54	92	of	of	ADP
ajlm-658	54	93	at	at	ADV
ajlm-658	54	94	least	least	ADV
ajlm-658	54	95	1	1	NUM
ajlm-658	54	96	%	%	NOUN
ajlm-658	54	97	to	to	PART
ajlm-658	54	98	ensure	ensure	VERB
ajlm-658	54	99	that	that	SCONJ
ajlm-658	54	100	more	more	ADJ
ajlm-658	54	101	than	than	ADP
ajlm-658	54	102	90	90	NUM
ajlm-658	54	103	%	%	NOUN
ajlm-658	54	104	of	of	ADP
ajlm-658	54	105	cases	case	NOUN
ajlm-658	54	106	are	be	AUX
ajlm-658	54	107	detected.13	detected.13	PRON
ajlm-658	54	108	our	our	PRON
ajlm-658	54	109	newly	newly	ADV
ajlm-658	54	110	designed	design	VERB
ajlm-658	54	111	lna	lna	PROPN
ajlm-658	54	112	probe	probe	NOUN
ajlm-658	54	113	,	,	PUNCT
ajlm-658	54	114	real	real	ADJ
ajlm-658	54	115	-	-	PUNCT
ajlm-658	54	116	time	time	NOUN
ajlm-658	54	117	pcr	pcr	NOUN
ajlm-658	54	118	assay	assay	NOUN
ajlm-658	54	119	detects	detect	NOUN
ajlm-658	54	120	up	up	ADV
ajlm-658	54	121	to	to	PART
ajlm-658	54	122	0.1	0.1	NUM
ajlm-658	54	123	%	%	NOUN
ajlm-658	54	124	of	of	ADP
ajlm-658	54	125	mutant	mutant	ADJ
ajlm-658	54	126	dna	dna	NOUN
ajlm-658	54	127	in	in	ADP
ajlm-658	54	128	a	a	DET
ajlm-658	54	129	wild	wild	ADJ
ajlm-658	54	130	-	-	PUNCT
ajlm-658	54	131	type	type	NOUN
ajlm-658	54	132	dna	dna	NOUN
ajlm-658	54	133	background	background	NOUN
ajlm-658	54	134	using	use	VERB
ajlm-658	54	135	gdna	gdna	PRON
ajlm-658	54	136	in	in	ADP
ajlm-658	54	137	contrast	contrast	NOUN
ajlm-658	54	138	to	to	ADP
ajlm-658	54	139	an	an	DET
ajlm-658	54	140	earlier	early	ADJ
ajlm-658	54	141	assay	assay	NOUN
ajlm-658	54	142	with	with	ADP
ajlm-658	54	143	an	an	DET
ajlm-658	54	144	analytical	analytical	ADJ
ajlm-658	54	145	sensitivity	sensitivity	NOUN
ajlm-658	54	146	of	of	ADP
ajlm-658	54	147	2	2	NUM
ajlm-658	54	148	%	%	NOUN
ajlm-658	54	149	using	use	VERB
ajlm-658	54	150	complementary	complementary	ADJ
ajlm-658	54	151	dna.19	dna.19	NOUN
ajlm-658	54	152	this	this	PRON
ajlm-658	54	153	may	may	AUX
ajlm-658	54	154	be	be	AUX
ajlm-658	54	155	an	an	DET
ajlm-658	54	156	improvement	improvement	NOUN
ajlm-658	54	157	on	on	ADP
ajlm-658	54	158	previously	previously	ADV
ajlm-658	54	159	published	publish	VERB
ajlm-658	54	160	probes	probe	NOUN
ajlm-658	54	161	.	.	PUNCT
ajlm-658	55	1	with	with	ADP
ajlm-658	55	2	the	the	DET
ajlm-658	55	3	good	good	ADJ
ajlm-658	55	4	analytical	analytical	ADJ
ajlm-658	55	5	sensitivity	sensitivity	NOUN
ajlm-658	55	6	of	of	ADP
ajlm-658	55	7	this	this	DET
ajlm-658	55	8	assay	assay	NOUN
ajlm-658	55	9	,	,	PUNCT
ajlm-658	55	10	it	it	PRON
ajlm-658	55	11	is	be	AUX
ajlm-658	55	12	considered	consider	VERB
ajlm-658	55	13	suitable	suitable	ADJ
ajlm-658	55	14	for	for	ADP
ajlm-658	55	15	use	use	NOUN
ajlm-658	55	16	in	in	ADP
ajlm-658	55	17	a	a	DET
ajlm-658	55	18	diagnostic	diagnostic	ADJ
ajlm-658	55	19	laboratory	laboratory	NOUN
ajlm-658	55	20	and	and	CCONJ
ajlm-658	55	21	constitutes	constitute	VERB
ajlm-658	55	22	a	a	DET
ajlm-658	55	23	good	good	ADJ
ajlm-658	55	24	screening	screening	NOUN
ajlm-658	55	25	tool	tool	NOUN
ajlm-658	55	26	.	.	PUNCT
ajlm-658	56	1	however	however	ADV
ajlm-658	56	2	,	,	PUNCT
ajlm-658	56	3	it	it	PRON
ajlm-658	56	4	should	should	AUX
ajlm-658	56	5	be	be	AUX
ajlm-658	56	6	noted	note	VERB
ajlm-658	56	7	that	that	SCONJ
ajlm-658	56	8	an	an	DET
ajlm-658	56	9	assay	assay	NOUN
ajlm-658	56	10	with	with	ADP
ajlm-658	56	11	this	this	DET
ajlm-658	56	12	sensitivity	sensitivity	NOUN
ajlm-658	56	13	may	may	AUX
ajlm-658	56	14	be	be	AUX
ajlm-658	56	15	prone	prone	ADJ
ajlm-658	56	16	to	to	PART
ajlm-658	56	17	give	give	VERB
ajlm-658	56	18	more	more	ADJ
ajlm-658	56	19	false	false	ADJ
ajlm-658	56	20	positives	positive	NOUN
ajlm-658	56	21	in	in	ADP
ajlm-658	56	22	a	a	DET
ajlm-658	56	23	diagnostic	diagnostic	ADJ
ajlm-658	56	24	setting.13	setting.13	NOUN
ajlm-658	56	25	limitations	limitation	NOUN
ajlm-658	56	26	to	to	PART
ajlm-658	56	27	assess	assess	VERB
ajlm-658	56	28	the	the	DET
ajlm-658	56	29	robustness	robustness	NOUN
ajlm-658	56	30	of	of	ADP
ajlm-658	56	31	a	a	DET
ajlm-658	56	32	real	real	ADJ
ajlm-658	56	33	-	-	PUNCT
ajlm-658	56	34	time	time	NOUN
ajlm-658	56	35	pcr	pcr	PROPN
ajlm-658	56	36	assay	assay	VERB
ajlm-658	56	37	different	different	ADJ
ajlm-658	56	38	experimental	experimental	ADJ
ajlm-658	56	39	conditions	condition	NOUN
ajlm-658	56	40	,	,	PUNCT
ajlm-658	56	41	such	such	ADJ
ajlm-658	56	42	as	as	ADP
ajlm-658	56	43	annealing	anneal	VERB
ajlm-658	56	44	temperatures	temperature	NOUN
ajlm-658	56	45	,	,	PUNCT
ajlm-658	56	46	the	the	DET
ajlm-658	56	47	use	use	NOUN
ajlm-658	56	48	of	of	ADP
ajlm-658	56	49	different	different	ADJ
ajlm-658	56	50	instruments	instrument	NOUN
ajlm-658	56	51	and	and	CCONJ
ajlm-658	56	52	operators	operator	NOUN
ajlm-658	56	53	should	should	AUX
ajlm-658	56	54	be	be	AUX
ajlm-658	56	55	introduced.14	introduced.14	PROPN
ajlm-658	56	56	this	this	DET
ajlm-658	56	57	aspect	aspect	NOUN
ajlm-658	56	58	of	of	ADP
ajlm-658	56	59	assay	assay	NOUN
ajlm-658	56	60	evaluation	evaluation	NOUN
ajlm-658	56	61	was	be	AUX
ajlm-658	56	62	not	not	PART
ajlm-658	56	63	explored	explore	VERB
ajlm-658	56	64	in	in	ADP
ajlm-658	56	65	this	this	DET
ajlm-658	56	66	study	study	NOUN
ajlm-658	56	67	.	.	PUNCT
ajlm-658	57	1	however	however	ADV
ajlm-658	57	2	,	,	PUNCT
ajlm-658	57	3	to	to	PART
ajlm-658	57	4	show	show	VERB
ajlm-658	57	5	that	that	SCONJ
ajlm-658	57	6	the	the	DET
ajlm-658	57	7	assay	assay	NOUN
ajlm-658	57	8	was	be	AUX
ajlm-658	57	9	able	able	ADJ
ajlm-658	57	10	to	to	PART
ajlm-658	57	11	perform	perform	VERB
ajlm-658	57	12	equally	equally	ADV
ajlm-658	57	13	well	well	ADV
ajlm-658	57	14	on	on	ADP
ajlm-658	57	15	another	another	DET
ajlm-658	57	16	platform	platform	NOUN
ajlm-658	57	17	,	,	PUNCT
ajlm-658	57	18	the	the	DET
ajlm-658	57	19	dna	dna	PROPN
ajlm-658	57	20	samples	sample	NOUN
ajlm-658	57	21	were	be	AUX
ajlm-658	57	22	analysed	analyse	VERB
ajlm-658	57	23	on	on	ADP
ajlm-658	57	24	the	the	DET
ajlm-658	57	25	qiagen	qiagen	NOUN
ajlm-658	57	26	rotor	rotor	NOUN
ajlm-658	57	27	-	-	PUNCT
ajlm-658	57	28	gene	gene	NOUN
ajlm-658	57	29	q	q	PROPN
ajlm-658	57	30	2plex	2plex	NUM
ajlm-658	57	31	system	system	NOUN
ajlm-658	57	32	(	(	PUNCT
ajlm-658	57	33	germany	germany	PROPN
ajlm-658	57	34	)	)	PUNCT
ajlm-658	57	35	.	.	PUNCT
ajlm-658	58	1	similar	similar	ADJ
ajlm-658	58	2	results	result	NOUN
ajlm-658	58	3	were	be	AUX
ajlm-658	58	4	obtained	obtain	VERB
ajlm-658	58	5	to	to	ADP
ajlm-658	58	6	that	that	PRON
ajlm-658	58	7	seen	see	VERB
ajlm-658	58	8	using	use	VERB
ajlm-658	58	9	the	the	DET
ajlm-658	58	10	cepheid	cepheid	PROPN
ajlm-658	58	11	smartcycler	smartcycler	PROPN
ajlm-658	58	12	ii	ii	PROPN
ajlm-658	58	13	system	system	NOUN
ajlm-658	58	14	.	.	PUNCT
ajlm-658	59	1	conclusion	conclusion	NOUN
ajlm-658	59	2	the	the	DET
ajlm-658	59	3	developed	develop	VERB
ajlm-658	59	4	lna	lna	PROPN
ajlm-658	59	5	probe	probe	NOUN
ajlm-658	59	6	,	,	PUNCT
ajlm-658	59	7	real	real	ADJ
ajlm-658	59	8	-	-	PUNCT
ajlm-658	59	9	time	time	NOUN
ajlm-658	59	10	pcr	pcr	PROPN
ajlm-658	59	11	assay	assay	NOUN
ajlm-658	59	12	is	be	AUX
ajlm-658	59	13	a	a	DET
ajlm-658	59	14	suitable	suitable	ADJ
ajlm-658	59	15	diagnostic	diagnostic	ADJ
ajlm-658	59	16	method	method	NOUN
ajlm-658	59	17	for	for	ADP
ajlm-658	59	18	detecting	detect	VERB
ajlm-658	59	19	the	the	DET
ajlm-658	59	20	jak2	jak2	PROPN
ajlm-658	59	21	v617f	v617f	PROPN
ajlm-658	59	22	mutation	mutation	NOUN
ajlm-658	59	23	in	in	ADP
ajlm-658	59	24	a	a	DET
ajlm-658	59	25	clinical	clinical	ADJ
ajlm-658	59	26	setting	setting	NOUN
ajlm-658	59	27	.	.	PUNCT
ajlm-658	60	1	it	it	PRON
ajlm-658	60	2	has	have	VERB
ajlm-658	60	3	good	good	ADJ
ajlm-658	60	4	sensitivity	sensitivity	NOUN
ajlm-658	60	5	,	,	PUNCT
ajlm-658	60	6	is	be	AUX
ajlm-658	60	7	easy	easy	ADJ
ajlm-658	60	8	to	to	PART
ajlm-658	60	9	set	set	VERB
ajlm-658	60	10	up	up	ADP
ajlm-658	60	11	and	and	CCONJ
ajlm-658	60	12	has	have	VERB
ajlm-658	60	13	rapid	rapid	ADJ
ajlm-658	60	14	turn	turn	NOUN
ajlm-658	60	15	-	-	PUNCT
ajlm-658	60	16	around	around	ADP
ajlm-658	60	17	times	time	NOUN
ajlm-658	60	18	.	.	PUNCT
ajlm-658	61	1	acknowledgements	acknowledgement	NOUN
ajlm-658	61	2	we	we	PRON
ajlm-658	61	3	would	would	AUX
ajlm-658	61	4	like	like	VERB
ajlm-658	61	5	to	to	PART
ajlm-658	61	6	thank	thank	VERB
ajlm-658	61	7	the	the	DET
ajlm-658	61	8	national	national	PROPN
ajlm-658	61	9	health	health	PROPN
ajlm-658	61	10	laboratory	laboratory	PROPN
ajlm-658	61	11	service	service	PROPN
ajlm-658	61	12	research	research	NOUN
ajlm-658	61	13	trust	trust	NOUN
ajlm-658	61	14	for	for	ADP
ajlm-658	61	15	funding	fund	VERB
ajlm-658	61	16	this	this	DET
ajlm-658	61	17	study	study	NOUN
ajlm-658	61	18	with	with	ADP
ajlm-658	61	19	a	a	DET
ajlm-658	61	20	pathology	pathology	NOUN
ajlm-658	61	21	research	research	NOUN
ajlm-658	61	22	development	development	NOUN
ajlm-658	61	23	grant	grant	NOUN
ajlm-658	61	24	.	.	PUNCT
ajlm-658	62	1	competing	compete	VERB
ajlm-658	62	2	interests	interest	NOUN
ajlm-658	62	3	the	the	DET
ajlm-658	62	4	authors	author	NOUN
ajlm-658	62	5	declare	declare	VERB
ajlm-658	62	6	that	that	SCONJ
ajlm-658	62	7	they	they	PRON
ajlm-658	62	8	have	have	VERB
ajlm-658	62	9	no	no	DET
ajlm-658	62	10	financial	financial	ADJ
ajlm-658	62	11	or	or	CCONJ
ajlm-658	62	12	personal	personal	ADJ
ajlm-658	62	13	relationships	relationship	NOUN
ajlm-658	62	14	which	which	PRON
ajlm-658	62	15	may	may	AUX
ajlm-658	62	16	have	have	AUX
ajlm-658	62	17	inappropriately	inappropriately	ADV
ajlm-658	62	18	influenced	influence	VERB
ajlm-658	62	19	them	they	PRON
ajlm-658	62	20	in	in	ADP
ajlm-658	62	21	writing	write	VERB
ajlm-658	62	22	this	this	DET
ajlm-658	62	23	article	article	NOUN
ajlm-658	62	24	.	.	PUNCT
ajlm-658	63	1	sources	source	NOUN
ajlm-658	63	2	of	of	ADP
ajlm-658	63	3	support	support	NOUN
ajlm-658	63	4	national	national	PROPN
ajlm-658	63	5	health	health	PROPN
ajlm-658	63	6	laboratory	laboratory	PROPN
ajlm-658	63	7	service	service	PROPN
ajlm-658	63	8	research	research	PROPN
ajlm-658	63	9	trust	trust	PROPN
ajlm-658	63	10	pathology	pathology	PROPN
ajlm-658	63	11	research	research	NOUN
ajlm-658	63	12	development	development	NOUN
ajlm-658	63	13	grant	grant	NOUN
ajlm-658	63	14	.	.	PUNCT
ajlm-658	64	1	authors	author	NOUN
ajlm-658	64	2	’	'	PUNCT
ajlm-658	64	3	contributions	contribution	NOUN
ajlm-658	64	4	j.j.c.p	j.j.c.p	PROPN
ajlm-658	64	5	.	.	PROPN
ajlm-658	64	6	,	,	PUNCT
ajlm-658	64	7	m.k	m.k	PROPN
ajlm-658	64	8	.	.	PROPN
ajlm-658	64	9	and	and	CCONJ
ajlm-658	64	10	t.s	t.s	PROPN
ajlm-658	64	11	.	.	PROPN
ajlm-658	64	12	were	be	AUX
ajlm-658	64	13	responsible	responsible	ADJ
ajlm-658	64	14	for	for	ADP
ajlm-658	64	15	the	the	DET
ajlm-658	64	16	experimental	experimental	ADJ
ajlm-658	64	17	and	and	CCONJ
ajlm-658	64	18	project	project	NOUN
ajlm-658	64	19	design	design	NOUN
ajlm-658	64	20	.	.	PUNCT
ajlm-658	65	1	t.s	t.s	PROPN
ajlm-658	65	2	.	.	PROPN
ajlm-658	65	3	performed	perform	VERB
ajlm-658	65	4	the	the	DET
ajlm-658	65	5	experiments	experiment	NOUN
ajlm-658	65	6	.	.	PUNCT
ajlm-658	66	1	p.b	p.b	ADJ
ajlm-658	66	2	.	.	PROPN
ajlm-658	67	1	and	and	CCONJ
ajlm-658	67	2	m.k	m.k	PROPN
ajlm-658	67	3	.	.	PROPN
ajlm-658	67	4	made	make	VERB
ajlm-658	67	5	conceptual	conceptual	ADJ
ajlm-658	67	6	contributions	contribution	NOUN
ajlm-658	67	7	.	.	PUNCT
ajlm-658	68	1	p.b	p.b	PROPN
ajlm-658	68	2	.	.	PROPN
ajlm-658	68	3	performed	perform	VERB
ajlm-658	68	4	the	the	DET
ajlm-658	68	5	calculations	calculation	NOUN
ajlm-658	68	6	.	.	PUNCT
ajlm-658	69	1	j.j.c.p	j.j.c.p	PROPN
ajlm-658	69	2	.	.	PROPN
ajlm-658	70	1	and	and	CCONJ
ajlm-658	70	2	t.s	t.s	PROPN
ajlm-658	70	3	.	.	PROPN
ajlm-658	70	4	wrote	write	VERB
ajlm-658	70	5	the	the	DET
ajlm-658	70	6	paper	paper	NOUN
ajlm-658	70	7	.	.	PUNCT
ajlm-658	71	1	j.j.c.p	j.j.c.p	PROPN
ajlm-658	71	2	.	.	PROPN
ajlm-658	71	3	was	be	AUX
ajlm-658	71	4	responsible	responsible	ADJ
ajlm-658	71	5	for	for	ADP
ajlm-658	71	6	funding	funding	NOUN
ajlm-658	71	7	acquisition	acquisition	NOUN
ajlm-658	71	8	.	.	PUNCT
ajlm-658	72	1	all	all	DET
ajlm-658	72	2	authors	author	NOUN
ajlm-658	72	3	read	read	VERB
ajlm-658	72	4	and	and	CCONJ
ajlm-658	72	5	approved	approve	VERB
ajlm-658	72	6	the	the	DET
ajlm-658	72	7	final	final	ADJ
ajlm-658	72	8	manuscript	manuscript	NOUN
ajlm-658	72	9	.	.	PUNCT
ajlm-658	73	1	references	reference	NOUN
ajlm-658	73	2	titmarsh	titmarsh	PROPN
ajlm-658	73	3	gj	gj	PROPN
ajlm-658	73	4	,	,	PUNCT
ajlm-658	73	5	duncombe	duncombe	NOUN
ajlm-658	73	6	as	as	SCONJ
ajlm-658	73	7	,	,	PUNCT
ajlm-658	73	8	mcmullin	mcmullin	PROPN
ajlm-658	73	9	mf	mf	VERB
ajlm-658	73	10	,	,	PUNCT
ajlm-658	73	11	et	et	PROPN
ajlm-658	73	12	al	al	PROPN
ajlm-658	73	13	.	.	PUNCT
ajlm-658	74	1	how	how	SCONJ
ajlm-658	74	2	common	common	ADJ
ajlm-658	74	3	are	be	AUX
ajlm-658	74	4	myeloproliferative	myeloproliferative	ADJ
ajlm-658	74	5	neoplasms	neoplasm	NOUN
ajlm-658	74	6	?	?	PUNCT
ajlm-658	75	1	a	a	DET
ajlm-658	75	2	systematic	systematic	ADJ
ajlm-658	75	3	review	review	NOUN
ajlm-658	75	4	and	and	CCONJ
ajlm-658	75	5	meta	meta	ADJ
ajlm-658	75	6	-	-	PUNCT
ajlm-658	75	7	analysis	analysis	NOUN
ajlm-658	75	8	.	.	PUNCT
ajlm-658	76	1	amj	amj	NOUN
ajlm-658	76	2	.	.	PUNCT
ajlm-658	77	1	2014;89(6):581–587	2014;89(6):581–587	NUM
ajlm-658	77	2	.	.	PUNCT
ajlm-658	78	1	https://doi.org/10.1002/ajh.23690	https://doi.org/10.1002/ajh.23690	PROPN
ajlm-658	78	2	baxter	baxter	PROPN
ajlm-658	78	3	ej	ej	PROPN
ajlm-658	78	4	,	,	PUNCT
ajlm-658	78	5	scott	scott	PROPN
ajlm-658	78	6	lm	lm	PROPN
ajlm-658	78	7	,	,	PUNCT
ajlm-658	78	8	campbell	campbell	PROPN
ajlm-658	78	9	pj	pj	PROPN
ajlm-658	78	10	,	,	PUNCT
ajlm-658	78	11	et	et	PROPN
ajlm-658	78	12	al	al	PROPN
ajlm-658	78	13	.	.	PROPN
ajlm-658	78	14	acquired	acquire	VERB
ajlm-658	78	15	mutation	mutation	NOUN
ajlm-658	78	16	of	of	ADP
ajlm-658	78	17	the	the	DET
ajlm-658	78	18	tyrosine	tyrosine	NOUN
ajlm-658	78	19	kinase	kinase	PROPN
ajlm-658	78	20	jak2	jak2	PROPN
ajlm-658	78	21	in	in	ADP
ajlm-658	78	22	human	human	ADJ
ajlm-658	78	23	myeloproliferative	myeloproliferative	ADJ
ajlm-658	78	24	disorders	disorder	NOUN
ajlm-658	78	25	.	.	PUNCT
ajlm-658	79	1	lancet	lancet	PROPN
ajlm-658	79	2	.	.	PUNCT
ajlm-658	80	1	2005;365(9464):1054–1061	2005;365(9464):1054–1061	PROPN
ajlm-658	80	2	.	.	PUNCT
ajlm-658	81	1	https://doi.org/10.1016/s0140-6736(05)74230-6	https://doi.org/10.1016/s0140-6736(05)74230-6	ADJ
ajlm-658	81	2	kralovics	kralovics	PROPN
ajlm-658	81	3	r	r	NOUN
ajlm-658	81	4	,	,	PUNCT
ajlm-658	81	5	passamonti	passamonti	NOUN
ajlm-658	81	6	f	f	NOUN
ajlm-658	81	7	,	,	PUNCT
ajlm-658	81	8	buser	buser	NOUN
ajlm-658	81	9	as	as	ADP
ajlm-658	81	10	,	,	PUNCT
ajlm-658	81	11	et	et	PROPN
ajlm-658	81	12	al	al	PROPN
ajlm-658	81	13	.	.	PUNCT
ajlm-658	82	1	a	a	DET
ajlm-658	82	2	gain	gain	VERB
ajlm-658	82	3	-	-	PUNCT
ajlm-658	82	4	of	of	ADP
ajlm-658	82	5	-	-	PUNCT
ajlm-658	82	6	function	function	NOUN
ajlm-658	82	7	mutation	mutation	NOUN
ajlm-658	82	8	of	of	ADP
ajlm-658	82	9	jak2	jak2	PROPN
ajlm-658	82	10	in	in	ADP
ajlm-658	82	11	myeloproliferative	myeloproliferative	ADJ
ajlm-658	82	12	disorders	disorder	NOUN
ajlm-658	82	13	.	.	PUNCT
ajlm-658	83	1	n	n	CCONJ
ajlm-658	83	2	engl	engl	PROPN
ajlm-658	83	3	j	j	PROPN
ajlm-658	83	4	med	med	PROPN
ajlm-658	83	5	.	.	PUNCT
ajlm-658	83	6	2005;352(17):1779–1790	2005;352(17):1779–1790	NUM
ajlm-658	83	7	.	.	PUNCT
ajlm-658	84	1	https://doi.org/10.1056/nejmoa051113	https://doi.org/10.1056/nejmoa051113	PROPN
ajlm-658	84	2	levine	levine	PROPN
ajlm-658	84	3	rl	rl	PROPN
ajlm-658	84	4	,	,	PUNCT
ajlm-658	84	5	wadleigh	wadleigh	NOUN
ajlm-658	84	6	m	m	PROPN
ajlm-658	84	7	,	,	PUNCT
ajlm-658	84	8	cools	cool	VERB
ajlm-658	84	9	j	j	PROPN
ajlm-658	84	10	,	,	PUNCT
ajlm-658	84	11	et	et	PROPN
ajlm-658	84	12	al	al	PROPN
ajlm-658	84	13	.	.	PUNCT
ajlm-658	85	1	activating	activate	VERB
ajlm-658	85	2	mutation	mutation	NOUN
ajlm-658	85	3	in	in	ADP
ajlm-658	85	4	the	the	DET
ajlm-658	85	5	tyrosine	tyrosine	NOUN
ajlm-658	85	6	kinase	kinase	PROPN
ajlm-658	85	7	jak2	jak2	PROPN
ajlm-658	85	8	in	in	ADP
ajlm-658	85	9	polycythemia	polycythemia	NOUN
ajlm-658	85	10	vera	vera	NOUN
ajlm-658	85	11	,	,	PUNCT
ajlm-658	85	12	essential	essential	ADJ
ajlm-658	85	13	thrombocythemia	thrombocythemia	NOUN
ajlm-658	85	14	,	,	PUNCT
ajlm-658	85	15	and	and	CCONJ
ajlm-658	85	16	myeloid	myeloid	ADJ
ajlm-658	85	17	metaplasia	metaplasia	NOUN
ajlm-658	85	18	with	with	ADP
ajlm-658	85	19	myelofibrosis	myelofibrosis	NOUN
ajlm-658	85	20	.	.	PUNCT
ajlm-658	86	1	cancer	cancer	NOUN
ajlm-658	86	2	cell	cell	NOUN
ajlm-658	86	3	.	.	PUNCT
ajlm-658	87	1	2005;7(4):387–397	2005;7(4):387–397	X
ajlm-658	87	2	.	.	X
ajlm-658	87	3	https://doi.org/10.1016/j.ccr.2005.03.023	https://doi.org/10.1016/j.ccr.2005.03.023	PROPN
ajlm-658	87	4	vardiman	vardiman	PROPN
ajlm-658	87	5	jw	jw	PROPN
ajlm-658	87	6	,	,	PUNCT
ajlm-658	87	7	thiele	thiele	PROPN
ajlm-658	87	8	j	j	PROPN
ajlm-658	87	9	,	,	PUNCT
ajlm-658	87	10	arber	arber	PROPN
ajlm-658	87	11	da	da	PROPN
ajlm-658	87	12	,	,	PUNCT
ajlm-658	87	13	et	et	PROPN
ajlm-658	87	14	al	al	PROPN
ajlm-658	87	15	.	.	PUNCT
ajlm-658	88	1	the	the	DET
ajlm-658	88	2	2008	2008	NUM
ajlm-658	88	3	revision	revision	NOUN
ajlm-658	88	4	of	of	ADP
ajlm-658	88	5	the	the	DET
ajlm-658	88	6	world	world	PROPN
ajlm-658	88	7	health	health	NOUN
ajlm-658	88	8	organization	organization	NOUN
ajlm-658	88	9	(	(	PUNCT
ajlm-658	88	10	who	who	PRON
ajlm-658	88	11	)	)	PUNCT
ajlm-658	88	12	classification	classification	NOUN
ajlm-658	88	13	of	of	ADP
ajlm-658	88	14	myeloid	myeloid	ADJ
ajlm-658	88	15	neoplasms	neoplasm	NOUN
ajlm-658	88	16	and	and	CCONJ
ajlm-658	88	17	acute	acute	ADJ
ajlm-658	88	18	leukemia	leukemia	NOUN
ajlm-658	88	19	:	:	PUNCT
ajlm-658	88	20	rationale	rationale	NOUN
ajlm-658	88	21	and	and	CCONJ
ajlm-658	88	22	important	important	ADJ
ajlm-658	88	23	changes	change	NOUN
ajlm-658	88	24	.	.	PUNCT
ajlm-658	89	1	blood	blood	NOUN
ajlm-658	89	2	.	.	PUNCT
ajlm-658	90	1	2009;114(5):937–951	2009;114(5):937–951	NUM
ajlm-658	90	2	.	.	PUNCT
ajlm-658	91	1	https://doi.org/10.1182/blood-2009-03-209262	https://doi.org/10.1182/blood-2009-03-209262	NUM
ajlm-658	91	2	langabeer	langabeer	PROPN
ajlm-658	91	3	se	se	PROPN
ajlm-658	91	4	,	,	PUNCT
ajlm-658	91	5	andrikovics	andrikovics	PROPN
ajlm-658	91	6	h	h	PROPN
ajlm-658	91	7	,	,	PUNCT
ajlm-658	91	8	asp	asp	PROPN
ajlm-658	91	9	j	j	PROPN
ajlm-658	91	10	,	,	PUNCT
ajlm-658	91	11	et	et	PROPN
ajlm-658	91	12	al	al	PROPN
ajlm-658	91	13	.	.	PUNCT
ajlm-658	92	1	molecular	molecular	ADJ
ajlm-658	92	2	diagnostics	diagnostic	NOUN
ajlm-658	92	3	of	of	ADP
ajlm-658	92	4	myeloproliferative	myeloproliferative	ADJ
ajlm-658	92	5	neoplasms	neoplasm	NOUN
ajlm-658	92	6	.	.	PUNCT
ajlm-658	93	1	eur	eur	PROPN
ajlm-658	93	2	j	j	PROPN
ajlm-658	93	3	haematol	haematol	NOUN
ajlm-658	93	4	.	.	PUNCT
ajlm-658	94	1	2015;95(4):270–279	2015;95(4):270–279	X
ajlm-658	94	2	.	.	PUNCT
ajlm-658	95	1	https://doi.org/10.1111/ejh.12578	https://doi.org/10.1111/ejh.12578	PROPN
ajlm-658	95	2	levine	levine	PROPN
ajlm-658	95	3	rl	rl	PROPN
ajlm-658	95	4	,	,	PUNCT
ajlm-658	95	5	gilliland	gilliland	PROPN
ajlm-658	95	6	dg	dg	PROPN
ajlm-658	95	7	.	.	PUNCT
ajlm-658	96	1	myeloproliferative	myeloproliferative	ADJ
ajlm-658	96	2	disorders	disorder	NOUN
ajlm-658	96	3	.	.	PUNCT
ajlm-658	97	1	blood	blood	NOUN
ajlm-658	97	2	.	.	PUNCT
ajlm-658	98	1	2008;112(6):2190–2198	2008;112(6):2190–2198	NUM
ajlm-658	98	2	.	.	PUNCT
ajlm-658	98	3	https://doi.org/10.1182/blood-2008-03-077966	https://doi.org/10.1182/blood-2008-03-077966	NUM
ajlm-658	99	1	quintás	quintás	NOUN
ajlm-658	99	2	-	-	PUNCT
ajlm-658	99	3	cardama	cardama	NOUN
ajlm-658	99	4	a	a	DET
ajlm-658	99	5	,	,	PUNCT
ajlm-658	99	6	vaddi	vaddi	ADJ
ajlm-658	99	7	k	k	PROPN
ajlm-658	99	8	,	,	PUNCT
ajlm-658	99	9	liu	liu	PROPN
ajlm-658	99	10	p	p	PROPN
ajlm-658	99	11	,	,	PUNCT
ajlm-658	99	12	et	et	PROPN
ajlm-658	99	13	al	al	PROPN
ajlm-658	99	14	.	.	PUNCT
ajlm-658	100	1	preclinical	preclinical	ADJ
ajlm-658	100	2	characterization	characterization	NOUN
ajlm-658	100	3	of	of	ADP
ajlm-658	100	4	the	the	DET
ajlm-658	100	5	selective	selective	ADJ
ajlm-658	100	6	jak1/2	jak1/2	PROPN
ajlm-658	100	7	inhibitor	inhibitor	NOUN
ajlm-658	100	8	incb018424	incb018424	PROPN
ajlm-658	100	9	:	:	PUNCT
ajlm-658	100	10	therapeutic	therapeutic	ADJ
ajlm-658	100	11	implications	implication	NOUN
ajlm-658	100	12	for	for	ADP
ajlm-658	100	13	the	the	DET
ajlm-658	100	14	treatment	treatment	NOUN
ajlm-658	100	15	of	of	ADP
ajlm-658	100	16	myeloproliferative	myeloproliferative	ADJ
ajlm-658	100	17	neoplasms	neoplasm	NOUN
ajlm-658	100	18	.	.	PUNCT
ajlm-658	101	1	blood	blood	NOUN
ajlm-658	101	2	.	.	PUNCT
ajlm-658	102	1	2010;115(15):3109–3117	2010;115(15):3109–3117	NUM
ajlm-658	102	2	.	.	PUNCT
ajlm-658	102	3	https://doi.org/10.1182/blood-2009-04-214957	https://doi.org/10.1182/blood-2009-04-214957	PROPN
ajlm-658	102	4	greiner	greiner	PROPN
ajlm-658	102	5	tc	tc	PROPN
ajlm-658	102	6	.	.	PUNCT
ajlm-658	103	1	diagnostic	diagnostic	ADJ
ajlm-658	103	2	assays	assay	NOUN
ajlm-658	103	3	for	for	ADP
ajlm-658	103	4	the	the	DET
ajlm-658	103	5	jak2	jak2	PROPN
ajlm-658	103	6	v617f	v617f	PROPN
ajlm-658	103	7	mutation	mutation	NOUN
ajlm-658	103	8	in	in	ADP
ajlm-658	103	9	chronic	chronic	ADJ
ajlm-658	103	10	myeloproliferative	myeloproliferative	ADJ
ajlm-658	103	11	disorders	disorder	NOUN
ajlm-658	103	12	.	.	PUNCT
ajlm-658	104	1	am	be	AUX
ajlm-658	104	2	j	j	PROPN
ajlm-658	104	3	clin	clin	PROPN
ajlm-658	104	4	path	path	NOUN
ajlm-658	104	5	.	.	PUNCT
ajlm-658	105	1	2006;125(5):651–653	2006;125(5):651–653	PROPN
ajlm-658	105	2	.	.	PROPN
ajlm-658	105	3	https://doi.org/10.1309/nxxtgrcxd0tma3c2	https://doi.org/10.1309/nxxtgrcxd0tma3c2	PROPN
ajlm-658	106	1	liu	liu	PROPN
ajlm-658	106	2	w	w	PROPN
ajlm-658	106	3	,	,	PUNCT
ajlm-658	106	4	hu	hu	PROPN
ajlm-658	106	5	t	t	PROPN
ajlm-658	106	6	,	,	PUNCT
ajlm-658	106	7	chen	chen	PROPN
ajlm-658	106	8	y	y	PROPN
ajlm-658	106	9	,	,	PUNCT
ajlm-658	106	10	zhang	zhang	PROPN
ajlm-658	106	11	x	x	PROPN
ajlm-658	106	12	,	,	PUNCT
ajlm-658	106	13	gu	gu	PROPN
ajlm-658	106	14	x	x	PROPN
ajlm-658	106	15	,	,	PUNCT
ajlm-658	106	16	guan	guan	PROPN
ajlm-658	106	17	m.	m.	PROPN
ajlm-658	106	18	development	development	PROPN
ajlm-658	106	19	and	and	CCONJ
ajlm-658	106	20	validation	validation	NOUN
ajlm-658	106	21	of	of	ADP
ajlm-658	106	22	a	a	DET
ajlm-658	106	23	tetra	tetra	NOUN
ajlm-658	106	24	-	-	PUNCT
ajlm-658	106	25	primer	primer	NOUN
ajlm-658	106	26	amplification	amplification	NOUN
ajlm-658	106	27	refractory	refractory	NOUN
ajlm-658	106	28	mutation	mutation	NOUN
ajlm-658	106	29	system	system	NOUN
ajlm-658	106	30	-	-	PUNCT
ajlm-658	106	31	polymerase	polymerase	NOUN
ajlm-658	106	32	chain	chain	NOUN
ajlm-658	106	33	reaction	reaction	NOUN
ajlm-658	106	34	combined	combine	VERB
ajlm-658	106	35	with	with	ADP
ajlm-658	106	36	melting	melt	VERB
ajlm-658	106	37	analysis	analysis	NOUN
ajlm-658	106	38	-	-	PUNCT
ajlm-658	106	39	assay	assay	NOUN
ajlm-658	106	40	for	for	ADP
ajlm-658	106	41	clinical	clinical	ADJ
ajlm-658	106	42	jak2	jak2	PROPN
ajlm-658	106	43	v617f	v617f	PROPN
ajlm-658	106	44	mutation	mutation	NOUN
ajlm-658	106	45	detection	detection	NOUN
ajlm-658	106	46	.	.	PUNCT
ajlm-658	107	1	mol	mol	ADP
ajlm-658	107	2	diagn	diagn	PROPN
ajlm-658	107	3	ther	ther	ADV
ajlm-658	107	4	.	.	PUNCT
ajlm-658	108	1	2014;18(5):579–585	2014;18(5):579–585	NUM
ajlm-658	108	2	.	.	PUNCT
ajlm-658	108	3	https://doi.org/10.1007/s40291-014-0111-6	https://doi.org/10.1007/s40291-014-0111-6	PROPN
ajlm-658	108	4	poodt	poodt	PROPN
ajlm-658	108	5	j	j	PROPN
ajlm-658	108	6	,	,	PUNCT
ajlm-658	108	7	fijnheer	fijnheer	NOUN
ajlm-658	108	8	r	r	PROPN
ajlm-658	108	9	,	,	PUNCT
ajlm-658	108	10	walsh	walsh	PROPN
ajlm-658	108	11	i	i	PROPN
ajlm-658	108	12	,	,	PUNCT
ajlm-658	108	13	hermans	hermans	PROPN
ajlm-658	108	14	m.	m.	VERB
ajlm-658	108	15	a	a	DET
ajlm-658	108	16	sensitive	sensitive	ADJ
ajlm-658	108	17	and	and	CCONJ
ajlm-658	108	18	reliable	reliable	ADJ
ajlm-658	108	19	semi	semi	ADJ
ajlm-658	108	20	-	-	ADJ
ajlm-658	108	21	quantitative	quantitative	ADJ
ajlm-658	108	22	real	real	ADJ
ajlm-658	108	23	-	-	PUNCT
ajlm-658	108	24	time	time	NOUN
ajlm-658	108	25	pcr	pcr	PROPN
ajlm-658	108	26	assay	assay	NOUN
ajlm-658	108	27	to	to	PART
ajlm-658	108	28	detect	detect	VERB
ajlm-658	108	29	jak2	jak2	PROPN
ajlm-658	108	30	v617f	v617f	PROPN
ajlm-658	108	31	in	in	ADP
ajlm-658	108	32	blood	blood	NOUN
ajlm-658	108	33	.	.	PUNCT
ajlm-658	109	1	hematol	hematol	NOUN
ajlm-658	109	2	oncol	oncol	ADJ
ajlm-658	109	3	.	.	PUNCT
ajlm-658	110	1	2006;24(4):227–233	2006;24(4):227–233	X
ajlm-658	110	2	.	.	PUNCT
ajlm-658	111	1	https://doi.org/10.1002/hon.800	https://doi.org/10.1002/hon.800	PROPN
ajlm-658	111	2	tan	tan	PROPN
ajlm-658	111	3	ay	ay	NOUN
ajlm-658	111	4	,	,	PUNCT
ajlm-658	111	5	westerman	westerman	PROPN
ajlm-658	111	6	da	da	PROPN
ajlm-658	111	7	,	,	PUNCT
ajlm-658	111	8	dobrovic	dobrovic	ADJ
ajlm-658	111	9	a.	a.	NOUN
ajlm-658	111	10	a	a	DET
ajlm-658	111	11	simple	simple	ADJ
ajlm-658	111	12	,	,	PUNCT
ajlm-658	111	13	rapid	rapid	ADJ
ajlm-658	111	14	,	,	PUNCT
ajlm-658	111	15	and	and	CCONJ
ajlm-658	111	16	sensitive	sensitive	ADJ
ajlm-658	111	17	method	method	NOUN
ajlm-658	111	18	for	for	ADP
ajlm-658	111	19	the	the	DET
ajlm-658	111	20	detection	detection	NOUN
ajlm-658	111	21	of	of	ADP
ajlm-658	111	22	the	the	DET
ajlm-658	111	23	jak2	jak2	PROPN
ajlm-658	111	24	v617f	v617f	PROPN
ajlm-658	111	25	mutation	mutation	NOUN
ajlm-658	111	26	.	.	PUNCT
ajlm-658	112	1	am	be	AUX
ajlm-658	112	2	j	j	PROPN
ajlm-658	112	3	clin	clin	PROPN
ajlm-658	112	4	path	path	NOUN
ajlm-658	112	5	.	.	PUNCT
ajlm-658	113	1	2007;127(6):977–981	2007;127(6):977–981	X
ajlm-658	113	2	.	.	X
ajlm-658	113	3	https://doi.org/10.1309/1u61jvxtlppq7yp1	https://doi.org/10.1309/1u61jvxtlppq7yp1	PROPN
ajlm-658	113	4	gong	gong	PROPN
ajlm-658	113	5	jz	jz	PROPN
ajlm-658	113	6	,	,	PUNCT
ajlm-658	113	7	cook	cook	VERB
ajlm-658	113	8	jr	jr	PROPN
ajlm-658	113	9	,	,	PUNCT
ajlm-658	113	10	greiner	greiner	PROPN
ajlm-658	113	11	tc	tc	PROPN
ajlm-658	113	12	,	,	PUNCT
ajlm-658	113	13	et	et	PROPN
ajlm-658	113	14	al	al	PROPN
ajlm-658	113	15	.	.	PROPN
ajlm-658	113	16	laboratory	laboratory	NOUN
ajlm-658	113	17	practice	practice	NOUN
ajlm-658	113	18	guidelines	guideline	NOUN
ajlm-658	113	19	for	for	ADP
ajlm-658	113	20	detecting	detect	VERB
ajlm-658	113	21	and	and	CCONJ
ajlm-658	113	22	reporting	report	VERB
ajlm-658	113	23	jak2	jak2	PROPN
ajlm-658	113	24	and	and	CCONJ
ajlm-658	113	25	mpl	mpl	PROPN
ajlm-658	113	26	mutations	mutation	NOUN
ajlm-658	113	27	in	in	ADP
ajlm-658	113	28	myeloproliferative	myeloproliferative	ADJ
ajlm-658	113	29	neoplasms	neoplasm	NOUN
ajlm-658	113	30	:	:	PUNCT
ajlm-658	113	31	a	a	DET
ajlm-658	113	32	report	report	NOUN
ajlm-658	113	33	of	of	ADP
ajlm-658	113	34	the	the	DET
ajlm-658	113	35	association	association	NOUN
ajlm-658	113	36	for	for	ADP
ajlm-658	113	37	molecular	molecular	ADJ
ajlm-658	113	38	pathology	pathology	NOUN
ajlm-658	113	39	.	.	PUNCT
ajlm-658	114	1	j	j	PROPN
ajlm-658	114	2	mol	mol	PROPN
ajlm-658	114	3	diagn	diagn	NOUN
ajlm-658	114	4	.	.	PUNCT
ajlm-658	115	1	2013;15(6):733–744	2013;15(6):733–744	X
ajlm-658	115	2	.	.	X
ajlm-658	115	3	https://doi.org/10.1016/j.jmoldx.2013.07.002	https://doi.org/10.1016/j.jmoldx.2013.07.002	NOUN
ajlm-658	115	4	broeders	broeder	NOUN
ajlm-658	115	5	s	s	PART
ajlm-658	115	6	,	,	PUNCT
ajlm-658	115	7	huber	huber	PROPN
ajlm-658	115	8	i	i	PROPN
ajlm-658	115	9	,	,	PUNCT
ajlm-658	115	10	grohmann	grohmann	PROPN
ajlm-658	115	11	l	l	PROPN
ajlm-658	115	12	,	,	PUNCT
ajlm-658	115	13	et	et	PROPN
ajlm-658	115	14	al	al	PROPN
ajlm-658	115	15	.	.	PUNCT
ajlm-658	116	1	guidelines	guideline	NOUN
ajlm-658	116	2	for	for	ADP
ajlm-658	116	3	validation	validation	NOUN
ajlm-658	116	4	of	of	ADP
ajlm-658	116	5	qualitative	qualitative	ADJ
ajlm-658	116	6	real	real	ADJ
ajlm-658	116	7	-	-	PUNCT
ajlm-658	116	8	time	time	NOUN
ajlm-658	116	9	pcr	pcr	ADJ
ajlm-658	116	10	methods	method	NOUN
ajlm-658	116	11	.	.	PUNCT
ajlm-658	117	1	trends	trend	NOUN
ajlm-658	117	2	food	food	NOUN
ajlm-658	117	3	sci	sci	PROPN
ajlm-658	117	4	technol	technol	NOUN
ajlm-658	117	5	.	.	PUNCT
ajlm-658	118	1	2014;37(2):115–126	2014;37(2):115–126	X
ajlm-658	118	2	.	.	PUNCT
ajlm-658	118	3	https://doi.org/10.1016/j.tifs.2014.03.008	https://doi.org/10.1016/j.tifs.2014.03.008	NOUN
ajlm-658	118	4	de	de	PROPN
ajlm-658	118	5	mast	mast	NOUN
ajlm-658	118	6	j.	j.	PROPN
ajlm-658	118	7	agreement	agreement	PROPN
ajlm-658	118	8	and	and	CCONJ
ajlm-658	118	9	kappa	kappa	ADJ
ajlm-658	118	10	-	-	ADJ
ajlm-658	118	11	type	type	NOUN
ajlm-658	118	12	indices	index	NOUN
ajlm-658	118	13	.	.	PUNCT
ajlm-658	119	1	am	be	AUX
ajlm-658	119	2	stat	stat	NOUN
ajlm-658	119	3	.	.	PUNCT
ajlm-658	120	1	2007;61(2):148–153	2007;61(2):148–153	NUM
ajlm-658	120	2	.	.	PUNCT
ajlm-658	120	3	https://doi.org/10.1198/000313007x192392	https://doi.org/10.1198/000313007x192392	X
ajlm-658	120	4	denys	denys	PROPN
ajlm-658	120	5	b	b	PROPN
ajlm-658	120	6	,	,	PUNCT
ajlm-658	120	7	el	el	PROPN
ajlm-658	120	8	housni	housni	PROPN
ajlm-658	120	9	h	h	PROPN
ajlm-658	120	10	,	,	PUNCT
ajlm-658	120	11	nollet	nollet	NOUN
ajlm-658	120	12	f	f	NOUN
ajlm-658	120	13	,	,	PUNCT
ajlm-658	120	14	verhasselt	verhasselt	PROPN
ajlm-658	120	15	b	b	NUM
ajlm-658	120	16	,	,	PUNCT
ajlm-658	120	17	philippé	philippé	ADJ
ajlm-658	120	18	j.	j.	PROPN
ajlm-658	120	19	a	a	DET
ajlm-658	120	20	real	real	ADJ
ajlm-658	120	21	-	-	PUNCT
ajlm-658	120	22	time	time	NOUN
ajlm-658	120	23	polymerase	polymerase	NOUN
ajlm-658	120	24	chain	chain	NOUN
ajlm-658	120	25	reaction	reaction	NOUN
ajlm-658	120	26	assay	assay	VERB
ajlm-658	120	27	for	for	ADP
ajlm-658	120	28	rapid	rapid	ADJ
ajlm-658	120	29	,	,	PUNCT
ajlm-658	120	30	sensitive	sensitive	ADJ
ajlm-658	120	31	,	,	PUNCT
ajlm-658	120	32	and	and	CCONJ
ajlm-658	120	33	specific	specific	ADJ
ajlm-658	120	34	quantification	quantification	NOUN
ajlm-658	120	35	of	of	ADP
ajlm-658	120	36	the	the	DET
ajlm-658	120	37	jak2v617f	jak2v617f	ADJ
ajlm-658	120	38	mutation	mutation	NOUN
ajlm-658	120	39	using	use	VERB
ajlm-658	120	40	a	a	DET
ajlm-658	120	41	locked	locked	ADJ
ajlm-658	120	42	nucleic	nucleic	ADJ
ajlm-658	120	43	acid	acid	NOUN
ajlm-658	120	44	-	-	PUNCT
ajlm-658	120	45	modified	modify	VERB
ajlm-658	120	46	oligonucleotide	oligonucleotide	NOUN
ajlm-658	120	47	.	.	PUNCT
ajlm-658	121	1	j	j	PROPN
ajlm-658	121	2	mol	mol	PROPN
ajlm-658	121	3	diagn	diagn	NOUN
ajlm-658	121	4	.	.	PUNCT
ajlm-658	122	1	2010;12(4):512–519	2010;12(4):512–519	X
ajlm-658	122	2	.	.	PUNCT
ajlm-658	123	1	https://doi.org/10.2353/jmoldx.2010.090137	https://doi.org/10.2353/jmoldx.2010.090137	PROPN
ajlm-658	123	2	simeonov	simeonov	PROPN
ajlm-658	123	3	a	a	PRON
ajlm-658	123	4	,	,	PUNCT
ajlm-658	123	5	nikiforov	nikiforov	PROPN
ajlm-658	123	6	tt	tt	PROPN
ajlm-658	123	7	.	.	PROPN
ajlm-658	123	8	single	single	ADJ
ajlm-658	123	9	nucleotide	nucleotide	ADJ
ajlm-658	123	10	polymorphism	polymorphism	NOUN
ajlm-658	123	11	genotyping	genotyping	NOUN
ajlm-658	123	12	using	use	VERB
ajlm-658	123	13	short	short	ADJ
ajlm-658	123	14	,	,	PUNCT
ajlm-658	123	15	fluorescently	fluorescently	ADV
ajlm-658	123	16	labeled	label	VERB
ajlm-658	123	17	locked	locked	ADJ
ajlm-658	123	18	nucleic	nucleic	ADJ
ajlm-658	123	19	acid	acid	NOUN
ajlm-658	123	20	(	(	PUNCT
ajlm-658	123	21	lna	lna	PROPN
ajlm-658	123	22	)	)	PUNCT
ajlm-658	123	23	probes	probe	NOUN
ajlm-658	123	24	and	and	CCONJ
ajlm-658	123	25	fluorescence	fluorescence	NOUN
ajlm-658	123	26	polarization	polarization	NOUN
ajlm-658	123	27	detection	detection	NOUN
ajlm-658	123	28	.	.	PUNCT
ajlm-658	124	1	nucleic	nucleic	ADJ
ajlm-658	124	2	acids	acid	NOUN
ajlm-658	124	3	res	re	NOUN
ajlm-658	124	4	.	.	PUNCT
ajlm-658	125	1	2002;30(17):e91	2002;30(17):e91	X
ajlm-658	125	2	.	.	PUNCT
ajlm-658	126	1	https://doi.org/10.1093/nar/gnf090	https://doi.org/10.1093/nar/gnf090	PROPN
ajlm-658	126	2	you	you	PRON
ajlm-658	126	3	y	y	PROPN
ajlm-658	126	4	,	,	PUNCT
ajlm-658	126	5	moreira	moreira	PROPN
ajlm-658	126	6	bg	bg	PROPN
ajlm-658	126	7	,	,	PUNCT
ajlm-658	126	8	behlke	behlke	PROPN
ajlm-658	126	9	ma	ma	PROPN
ajlm-658	126	10	,	,	PUNCT
ajlm-658	126	11	owczarzy	owczarzy	PROPN
ajlm-658	126	12	r.	r.	PROPN
ajlm-658	126	13	design	design	PROPN
ajlm-658	126	14	of	of	ADP
ajlm-658	126	15	lna	lna	PROPN
ajlm-658	126	16	probes	probe	NOUN
ajlm-658	126	17	that	that	PRON
ajlm-658	126	18	improve	improve	VERB
ajlm-658	126	19	mismatch	mismatch	ADJ
ajlm-658	126	20	discrimination	discrimination	NOUN
ajlm-658	126	21	.	.	PUNCT
ajlm-658	127	1	nucleic	nucleic	ADJ
ajlm-658	127	2	acids	acid	NOUN
ajlm-658	127	3	res	re	NOUN
ajlm-658	127	4	.	.	PUNCT
ajlm-658	128	1	2006;34(8):e60	2006;34(8):e60	X
ajlm-658	128	2	.	.	PUNCT
ajlm-658	129	1	https://doi.org/10.1093/nar/gkl175	https://doi.org/10.1093/nar/gkl175	PROPN
ajlm-658	129	2	marková	marková	VERB
ajlm-658	129	3	j	j	PROPN
ajlm-658	129	4	,	,	PUNCT
ajlm-658	129	5	průková	průková	NOUN
ajlm-658	129	6	d	d	NOUN
ajlm-658	129	7	,	,	PUNCT
ajlm-658	129	8	volková	volková	PROPN
ajlm-658	129	9	z	z	PROPN
ajlm-658	129	10	,	,	PUNCT
ajlm-658	129	11	schwarz	schwarz	PROPN
ajlm-658	129	12	j.	j.	PROPN
ajlm-658	129	13	a	a	DET
ajlm-658	129	14	new	new	ADJ
ajlm-658	129	15	allelic	allelic	ADJ
ajlm-658	129	16	discrimination	discrimination	NOUN
ajlm-658	129	17	assay	assay	NOUN
ajlm-658	129	18	using	use	VERB
ajlm-658	129	19	locked	lock	VERB
ajlm-658	129	20	nucleic	nucleic	ADJ
ajlm-658	129	21	acid	acid	NOUN
ajlm-658	129	22	-	-	PUNCT
ajlm-658	129	23	modified	modify	VERB
ajlm-658	129	24	nucleotides	nucleotide	NOUN
ajlm-658	129	25	(	(	PUNCT
ajlm-658	129	26	lna	lna	PROPN
ajlm-658	129	27	)	)	PUNCT
ajlm-658	129	28	probes	probe	NOUN
ajlm-658	129	29	for	for	ADP
ajlm-658	129	30	detection	detection	NOUN
ajlm-658	129	31	of	of	ADP
ajlm-658	129	32	jak2	jak2	PROPN
ajlm-658	129	33	v617f	v617f	PROPN
ajlm-658	129	34	mutation	mutation	NOUN
ajlm-658	129	35	.	.	PUNCT
ajlm-658	130	1	leuk	leuk	PROPN
ajlm-658	130	2	lymphoma	lymphoma	NOUN
ajlm-658	130	3	.	.	PUNCT
ajlm-658	131	1	2007;48(3):636–639	2007;48(3):636–639	X
ajlm-658	131	2	.	.	PUNCT
ajlm-658	131	3	https://doi.org/10.1080/10428190601137328	https://doi.org/10.1080/10428190601137328	VERB