id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
ajpf-757	Suchitra Muenthaisong; Andras Dinnyes; Tshimangadzo Lucky Nedambale	Review of somatic cell nuclear transfer in pig	2018	7	.pdf	application/pdf	5175	380	62	In the case of chemical activation, the oocytes were exposed to the substrates such as cytochalasin B (Li et al., 2000; Park et al., 2002; Hoshino et al., 2005) to prevent the extrusion of the second polar body, cycloheximide (Lee et al., 2003a) or 6- dimethylaminopurine (DMAP; de la Fuente and King, 1998; Hölker et al., 2005) to inhibit the protein kinase after an induced calcium transient to promote activation or ionomycin (Betthauser et al., 2000; Boquest et al., 2002) that it forms a complex with calcium ions and transports through the plasma membrane and it can also stimulate calcium and induce a calcium influx similar electrical activation (Morgan and Jacob, 1994). Many strategies to introduce the donor cell into cytoplasm of the recipient cytoplasm such as transferred by electrofusion (Polejaeva et al., 2000), by piezo-microinjection of isolated donor nuclei (Onishi et al., 2000), and also by whole cell injection (Lee et al., 2003b).	cache/ajpf-757.pdf	txt/ajpf-757.txt