id	sid	tid	token	lemma	pos
ajpf-645	1	1	in	in	ADP
ajpf-645	1	2	ternationa	ternationa	NOUN
ajpf-645	1	3	l	l	NOUN
ajpf-645	1	4	scholars	scholar	NOUN
ajpf-645	1	5	journa	journa	VERB
ajpf-645	1	6	ls	ls	PROPN
ajpf-645	1	7	african	african	ADJ
ajpf-645	1	8	journal	journal	PROPN
ajpf-645	1	9	of	of	ADP
ajpf-645	1	10	pig	pig	PROPN
ajpf-645	1	11	farming	farming	PROPN
ajpf-645	1	12	issn	issn	PROPN
ajpf-645	1	13	2375	2375	NUM
ajpf-645	1	14	-	-	SYM
ajpf-645	1	15	0731	0731	NUM
ajpf-645	1	16	vol	vol	NOUN
ajpf-645	1	17	.	.	NOUN
ajpf-645	1	18	3	3	NUM
ajpf-645	1	19	(	(	PUNCT
ajpf-645	1	20	1	1	NUM
ajpf-645	1	21	)	)	PUNCT
ajpf-645	1	22	,	,	PUNCT
ajpf-645	1	23	pp	pp	PROPN
ajpf-645	1	24	.	.	PUNCT
ajpf-645	2	1	001	001	NUM
ajpf-645	2	2	-	-	PUNCT
ajpf-645	2	3	009	009	NUM
ajpf-645	2	4	,	,	PUNCT
ajpf-645	2	5	january	january	PROPN
ajpf-645	2	6	,	,	PUNCT
ajpf-645	2	7	2015	2015	NUM
ajpf-645	2	8	.	.	PUNCT
ajpf-645	3	1	available	available	ADJ
ajpf-645	3	2	online	online	ADV
ajpf-645	3	3	at	at	ADP
ajpf-645	3	4	www.internationalscholarsjournals.org	www.internationalscholarsjournals.org	PROPN
ajpf-645	3	5	©	©	PROPN
ajpf-645	3	6	international	international	ADJ
ajpf-645	3	7	scholars	scholar	NOUN
ajpf-645	3	8	journals	journal	NOUN
ajpf-645	3	9	author(s	author(s	PROPN
ajpf-645	3	10	)	)	PUNCT
ajpf-645	3	11	retain	retain	VERB
ajpf-645	3	12	the	the	DET
ajpf-645	3	13	copyright	copyright	NOUN
ajpf-645	3	14	of	of	ADP
ajpf-645	3	15	this	this	DET
ajpf-645	3	16	article	article	NOUN
ajpf-645	3	17	.	.	PUNCT
ajpf-645	4	1	full	full	ADJ
ajpf-645	4	2	length	length	NOUN
ajpf-645	4	3	research	research	NOUN
ajpf-645	4	4	paper	paper	NOUN
ajpf-645	4	5	an	an	DET
ajpf-645	4	6	efficient	efficient	ADJ
ajpf-645	4	7	secretion	secretion	NOUN
ajpf-645	4	8	of	of	ADP
ajpf-645	4	9	the	the	DET
ajpf-645	4	10	protein	protein	NOUN
ajpf-645	4	11	fused	fuse	VERB
ajpf-645	4	12	to	to	ADP
ajpf-645	4	13	the	the	DET
ajpf-645	4	14	agfa	agfa	PROPN
ajpf-645	4	15	signal	signal	PROPN
ajpf-645	4	16	sequence	sequence	NOUN
ajpf-645	4	17	in	in	ADP
ajpf-645	4	18	salmonella	salmonella	NOUN
ajpf-645	4	19	ho	ho	PROPN
ajpf-645	4	20	young	young	ADJ
ajpf-645	4	21	kang1	kang1	PROPN
ajpf-645	4	22	,	,	PUNCT
ajpf-645	4	23	ki	ki	PROPN
ajpf-645	4	24	hwan	hwan	PROPN
ajpf-645	4	25	moon1	moon1	PROPN
ajpf-645	4	26	,	,	PUNCT
ajpf-645	4	27	se	se	PROPN
ajpf-645	4	28	won	win	VERB
ajpf-645	4	29	kim2	kim2	PROPN
ajpf-645	4	30	,	,	PUNCT
ajpf-645	4	31	jeong	jeong	PROPN
ajpf-645	4	32	dong	dong	PROPN
ajpf-645	4	33	bahk	bahk	PROPN
ajpf-645	4	34	2	2	NUM
ajpf-645	4	35	,	,	PUNCT
ajpf-645	4	36	sang	sing	VERB
ajpf-645	4	37	wan	wan	PROPN
ajpf-645	4	38	gal3	gal3	PROPN
ajpf-645	4	39	,	,	PUNCT
ajpf-645	4	40	kwangkeun	kwangkeun	PROPN
ajpf-645	4	41	cho4	cho4	PROPN
ajpf-645	4	42	,	,	PUNCT
ajpf-645	4	43	chul	chul	ADJ
ajpf-645	4	44	-	-	PUNCT
ajpf-645	4	45	wook	wook	NOUN
ajpf-645	4	46	kim4	kim4	PROPN
ajpf-645	4	47	,	,	PUNCT
ajpf-645	4	48	john	john	PROPN
ajpf-645	4	49	hwa	hwa	PROPN
ajpf-645	4	50	lee5	lee5	PROPN
ajpf-645	4	51	and	and	CCONJ
ajpf-645	4	52	sam	sam	PROPN
ajpf-645	4	53	woong	woong	PROPN
ajpf-645	4	54	kim6	kim6	PROPN
ajpf-645	4	55	*	*	PROPN
ajpf-645	4	56	1	1	NUM
ajpf-645	4	57	department	department	NOUN
ajpf-645	4	58	of	of	ADP
ajpf-645	4	59	microbiology	microbiology	NOUN
ajpf-645	4	60	,	,	PUNCT
ajpf-645	4	61	college	college	NOUN
ajpf-645	4	62	of	of	ADP
ajpf-645	4	63	natural	natural	ADJ
ajpf-645	4	64	sciences	science	NOUN
ajpf-645	4	65	,	,	PUNCT
ajpf-645	4	66	pusan	pusan	PROPN
ajpf-645	4	67	national	national	PROPN
ajpf-645	4	68	university	university	PROPN
ajpf-645	4	69	,	,	PUNCT
ajpf-645	4	70	busan	busan	PROPN
ajpf-645	4	71	609	609	NUM
ajpf-645	4	72	-	-	SYM
ajpf-645	4	73	735	735	NUM
ajpf-645	4	74	,	,	PUNCT
ajpf-645	4	75	south	south	PROPN
ajpf-645	4	76	korea	korea	PROPN
ajpf-645	4	77	.	.	PUNCT
ajpf-645	5	1	2	2	NUM
ajpf-645	5	2	division	division	NOUN
ajpf-645	5	3	of	of	ADP
ajpf-645	5	4	applied	apply	VERB
ajpf-645	5	5	life	life	NOUN
ajpf-645	5	6	sciences	science	NOUN
ajpf-645	5	7	(	(	PUNCT
ajpf-645	5	8	bk21	bk21	PROPN
ajpf-645	5	9	-	-	PUNCT
ajpf-645	5	10	ebncrc	ebncrc	PROPN
ajpf-645	5	11	)	)	PUNCT
ajpf-645	5	12	,	,	PUNCT
ajpf-645	5	13	graduate	graduate	NOUN
ajpf-645	5	14	school	school	NOUN
ajpf-645	5	15	of	of	ADP
ajpf-645	5	16	gyeongsang	gyeongsang	PROPN
ajpf-645	5	17	national	national	PROPN
ajpf-645	5	18	university	university	PROPN
ajpf-645	5	19	,	,	PUNCT
ajpf-645	5	20	jinju	jinju	NOUN
ajpf-645	5	21	660	660	NUM
ajpf-645	5	22	-	-	SYM
ajpf-645	5	23	701	701	NUM
ajpf-645	5	24	,	,	PUNCT
ajpf-645	5	25	korea	korea	PROPN
ajpf-645	5	26	.	.	PUNCT
ajpf-645	6	1	3	3	NUM
ajpf-645	6	2	department	department	NOUN
ajpf-645	6	3	of	of	ADP
ajpf-645	6	4	pharmaceutical	pharmaceutical	ADJ
ajpf-645	6	5	engineering	engineering	NOUN
ajpf-645	6	6	,	,	PUNCT
ajpf-645	6	7	gyeongnam	gyeongnam	PROPN
ajpf-645	6	8	national	national	PROPN
ajpf-645	6	9	university	university	PROPN
ajpf-645	6	10	of	of	ADP
ajpf-645	6	11	science	science	NOUN
ajpf-645	6	12	and	and	CCONJ
ajpf-645	6	13	technology	technology	NOUN
ajpf-645	6	14	,	,	PUNCT
ajpf-645	6	15	150	150	NUM
ajpf-645	6	16	chilam	chilam	PROPN
ajpf-645	6	17	dong	dong	NOUN
ajpf-645	6	18	,	,	PUNCT
ajpf-645	6	19	jinju	jinju	NOUN
ajpf-645	6	20	660	660	NUM
ajpf-645	6	21	-	-	SYM
ajpf-645	6	22	758	758	NUM
ajpf-645	6	23	,	,	PUNCT
ajpf-645	6	24	korea	korea	PROPN
ajpf-645	6	25	.	.	PUNCT
ajpf-645	7	1	4	4	NUM
ajpf-645	7	2	department	department	NOUN
ajpf-645	7	3	of	of	ADP
ajpf-645	7	4	animal	animal	NOUN
ajpf-645	7	5	resources	resource	NOUN
ajpf-645	7	6	technology	technology	NOUN
ajpf-645	7	7	,	,	PUNCT
ajpf-645	7	8	gyeongnam	gyeongnam	PROPN
ajpf-645	7	9	national	national	PROPN
ajpf-645	7	10	university	university	PROPN
ajpf-645	7	11	of	of	ADP
ajpf-645	7	12	science	science	NOUN
ajpf-645	7	13	and	and	CCONJ
ajpf-645	7	14	technology	technology	NOUN
ajpf-645	7	15	,	,	PUNCT
ajpf-645	7	16	150	150	NUM
ajpf-645	7	17	chilam	chilam	PROPN
ajpf-645	7	18	dong	dong	NOUN
ajpf-645	7	19	,	,	PUNCT
ajpf-645	7	20	jinju	jinju	NOUN
ajpf-645	7	21	,	,	PUNCT
ajpf-645	7	22	gyeongnam	gyeongnam	ADJ
ajpf-645	7	23	,	,	PUNCT
ajpf-645	7	24	660	660	NUM
ajpf-645	7	25	-	-	SYM
ajpf-645	7	26	758	758	NUM
ajpf-645	7	27	,	,	PUNCT
ajpf-645	7	28	south	south	PROPN
ajpf-645	7	29	korea	korea	PROPN
ajpf-645	7	30	.	.	PUNCT
ajpf-645	8	1	5	5	NUM
ajpf-645	8	2	college	college	NOUN
ajpf-645	8	3	of	of	ADP
ajpf-645	8	4	veterinary	veterinary	ADJ
ajpf-645	8	5	medicine	medicine	NOUN
ajpf-645	8	6	,	,	PUNCT
ajpf-645	8	7	chonbuk	chonbuk	ADJ
ajpf-645	8	8	national	national	PROPN
ajpf-645	8	9	university	university	PROPN
ajpf-645	8	10	,	,	PUNCT
ajpf-645	8	11	chon	chon	PROPN
ajpf-645	8	12	-	-	PUNCT
ajpf-645	8	13	ju	ju	PROPN
ajpf-645	8	14	561	561	NUM
ajpf-645	8	15	-	-	SYM
ajpf-645	8	16	756	756	NUM
ajpf-645	8	17	,	,	PUNCT
ajpf-645	8	18	south	south	PROPN
ajpf-645	8	19	korea	korea	PROPN
ajpf-645	8	20	.	.	PUNCT
ajpf-645	9	1	6	6	NUM
ajpf-645	9	2	swine	swine	ADJ
ajpf-645	9	3	science	science	NOUN
ajpf-645	9	4	and	and	CCONJ
ajpf-645	9	5	technology	technology	NOUN
ajpf-645	9	6	center	center	NOUN
ajpf-645	9	7	,	,	PUNCT
ajpf-645	9	8	gyeongnam	gyeongnam	PROPN
ajpf-645	9	9	national	national	PROPN
ajpf-645	9	10	university	university	PROPN
ajpf-645	9	11	of	of	ADP
ajpf-645	9	12	science	science	NOUN
ajpf-645	9	13	and	and	CCONJ
ajpf-645	9	14	technology	technology	NOUN
ajpf-645	9	15	,	,	PUNCT
ajpf-645	9	16	150	150	NUM
ajpf-645	9	17	chilam	chilam	NOUN
ajpf-645	9	18	-	-	PUNCT
ajpf-645	9	19	dong	dong	NOUN
ajpf-645	9	20	,	,	PUNCT
ajpf-645	9	21	jinju	jinju	NOUN
ajpf-645	9	22	,	,	PUNCT
ajpf-645	9	23	gyeongnam	gyeongnam	ADJ
ajpf-645	9	24	,	,	PUNCT
ajpf-645	9	25	660	660	NUM
ajpf-645	9	26	-	-	SYM
ajpf-645	9	27	758	758	NUM
ajpf-645	9	28	,	,	PUNCT
ajpf-645	9	29	south	south	PROPN
ajpf-645	9	30	korea	korea	PROPN
ajpf-645	9	31	.	.	PUNCT
ajpf-645	10	1	accepted	accept	VERB
ajpf-645	10	2	02	02	NUM
ajpf-645	10	3	december	december	PROPN
ajpf-645	10	4	,	,	PUNCT
ajpf-645	10	5	2014	2014	NUM
ajpf-645	10	6	signal	signal	NOUN
ajpf-645	10	7	sequence	sequence	NOUN
ajpf-645	10	8	(	(	PUNCT
ajpf-645	10	9	ss	ss	NOUN
ajpf-645	10	10	)	)	PUNCT
ajpf-645	10	11	of	of	ADP
ajpf-645	10	12	surface	surface	NOUN
ajpf-645	10	13	or	or	CCONJ
ajpf-645	10	14	secreting	secrete	VERB
ajpf-645	10	15	proteins	protein	NOUN
ajpf-645	10	16	plays	play	VERB
ajpf-645	10	17	an	an	DET
ajpf-645	10	18	important	important	ADJ
ajpf-645	10	19	function	function	NOUN
ajpf-645	10	20	for	for	ADP
ajpf-645	10	21	protein	protein	NOUN
ajpf-645	10	22	secretion	secretion	NOUN
ajpf-645	10	23	in	in	ADP
ajpf-645	10	24	bacterial	bacterial	ADJ
ajpf-645	10	25	system	system	NOUN
ajpf-645	10	26	.	.	PUNCT
ajpf-645	11	1	the	the	DET
ajpf-645	11	2	ss	ss	NOUN
ajpf-645	11	3	of	of	ADP
ajpf-645	11	4	various	various	ADJ
ajpf-645	11	5	proteins	protein	NOUN
ajpf-645	11	6	may	may	AUX
ajpf-645	11	7	mediate	mediate	VERB
ajpf-645	11	8	different	different	ADJ
ajpf-645	11	9	level	level	NOUN
ajpf-645	11	10	of	of	ADP
ajpf-645	11	11	protein	protein	NOUN
ajpf-645	11	12	excretion	excretion	NOUN
ajpf-645	11	13	yield	yield	NOUN
ajpf-645	11	14	of	of	ADP
ajpf-645	11	15	the	the	DET
ajpf-645	11	16	proteins	protein	NOUN
ajpf-645	11	17	.	.	PUNCT
ajpf-645	12	1	in	in	ADP
ajpf-645	12	2	order	order	NOUN
ajpf-645	12	3	to	to	PART
ajpf-645	12	4	examine	examine	VERB
ajpf-645	12	5	the	the	DET
ajpf-645	12	6	effect	effect	NOUN
ajpf-645	12	7	of	of	ADP
ajpf-645	12	8	ss	ss	NOUN
ajpf-645	12	9	types	type	NOUN
ajpf-645	12	10	in	in	ADP
ajpf-645	12	11	protein	protein	NOUN
ajpf-645	12	12	secretion	secretion	NOUN
ajpf-645	12	13	,	,	PUNCT
ajpf-645	12	14	signal	signal	VERB
ajpf-645	12	15	sequences	sequence	NOUN
ajpf-645	12	16	of	of	ADP
ajpf-645	12	17	bla	bla	NOUN
ajpf-645	12	18	(	(	PUNCT
ajpf-645	12	19			NOUN
ajpf-645	12	20	–	–	PUNCT
ajpf-645	12	21	lactamase	lactamase	NOUN
ajpf-645	12	22	)	)	PUNCT
ajpf-645	12	23	,	,	PUNCT
ajpf-645	12	24	agfa	agfa	PROPN
ajpf-645	12	25	(	(	PUNCT
ajpf-645	12	26	thin	thin	PROPN
ajpf-645	12	27	aggregative	aggregative	ADJ
ajpf-645	12	28	fimbriae	fimbriae	NOUN
ajpf-645	12	29	a	a	X
ajpf-645	12	30	)	)	PUNCT
ajpf-645	12	31	,	,	PUNCT
ajpf-645	12	32	stfa	stfa	NOUN
ajpf-645	12	33	(	(	PUNCT
ajpf-645	12	34	salmonella	salmonella	NOUN
ajpf-645	12	35	typhimurium	typhimurium	PRON
ajpf-645	12	36	fimbriae	fimbriae	NOUN
ajpf-645	12	37	a	a	NOUN
ajpf-645	12	38	)	)	PUNCT
ajpf-645	12	39	and	and	CCONJ
ajpf-645	12	40	ompw	ompw	NOUN
ajpf-645	12	41	(	(	PUNCT
ajpf-645	12	42	outer	outer	ADJ
ajpf-645	12	43	membrane	membrane	NOUN
ajpf-645	12	44	protein	protein	NOUN
ajpf-645	12	45	w	w	NOUN
ajpf-645	12	46	)	)	PUNCT
ajpf-645	12	47	were	be	AUX
ajpf-645	12	48	selected	select	VERB
ajpf-645	12	49	for	for	ADP
ajpf-645	12	50	the	the	DET
ajpf-645	12	51	secretion	secretion	NOUN
ajpf-645	12	52	of	of	ADP
ajpf-645	12	53	pspa	pspa	ADJ
ajpf-645	12	54	protein	protein	NOUN
ajpf-645	12	55	which	which	PRON
ajpf-645	12	56	was	be	AUX
ajpf-645	12	57	used	use	VERB
ajpf-645	12	58	as	as	ADP
ajpf-645	12	59	a	a	DET
ajpf-645	12	60	test	test	NOUN
ajpf-645	12	61	protein	protein	NOUN
ajpf-645	12	62	.	.	PUNCT
ajpf-645	13	1	the	the	DET
ajpf-645	13	2	pcr	pcr	NOUN
ajpf-645	13	3	-	-	PUNCT
ajpf-645	13	4	amplified	amplify	VERB
ajpf-645	13	5	dnas	dna	NOUN
ajpf-645	13	6	corresponding	correspond	VERB
ajpf-645	13	7	to	to	ADP
ajpf-645	13	8	each	each	DET
ajpf-645	13	9	ss	ss	PROPN
ajpf-645	13	10	were	be	AUX
ajpf-645	13	11	cloned	clone	VERB
ajpf-645	13	12	into	into	ADP
ajpf-645	13	13	the	the	DET
ajpf-645	13	14	plasmid	plasmid	ADJ
ajpf-645	13	15	pya3342	pya3342	NOUN
ajpf-645	13	16	.	.	PUNCT
ajpf-645	14	1	a	a	DET
ajpf-645	14	2	primer	primer	NOUN
ajpf-645	14	3	used	use	VERB
ajpf-645	14	4	in	in	ADP
ajpf-645	14	5	pcr	pcr	PROPN
ajpf-645	14	6	was	be	AUX
ajpf-645	14	7	designed	design	VERB
ajpf-645	14	8	to	to	PART
ajpf-645	14	9	insert	insert	VERB
ajpf-645	14	10	a	a	DET
ajpf-645	14	11	his6	his6	NOUN
ajpf-645	14	12	-	-	PUNCT
ajpf-645	14	13	tag	tag	NOUN
ajpf-645	14	14	at	at	ADP
ajpf-645	14	15	the	the	DET
ajpf-645	14	16	cterminal	cterminal	NOUN
ajpf-645	14	17	of	of	ADP
ajpf-645	14	18	ss	ss	NOUN
ajpf-645	14	19	for	for	ADP
ajpf-645	14	20	the	the	DET
ajpf-645	14	21	convenient	convenient	ADJ
ajpf-645	14	22	detection	detection	NOUN
ajpf-645	14	23	of	of	ADP
ajpf-645	14	24	expressed	express	VERB
ajpf-645	14	25	ss	ss	PROPN
ajpf-645	14	26	.	.	PUNCT
ajpf-645	15	1	the	the	DET
ajpf-645	15	2	0.8	0.8	NUM
ajpf-645	15	3	kb	kb	PROPN
ajpf-645	15	4	ecori	ecori	PROPN
ajpf-645	15	5	-	-	PUNCT
ajpf-645	15	6	hin	hin	PROPN
ajpf-645	15	7	diii	diii	PROPN
ajpf-645	15	8	pspa	pspa	PROPN
ajpf-645	15	9	gene	gene	NOUN
ajpf-645	15	10	was	be	AUX
ajpf-645	15	11	cloned	clone	VERB
ajpf-645	15	12	into	into	ADP
ajpf-645	15	13	the	the	DET
ajpf-645	15	14	recombinant	recombinant	ADJ
ajpf-645	15	15	plasmids	plasmid	NOUN
ajpf-645	15	16	,	,	PUNCT
ajpf-645	15	17	resulting	result	VERB
ajpf-645	15	18	pmmp66	pmmp66	NOUN
ajpf-645	15	19	,	,	PUNCT
ajpf-645	15	20	pmmp67	pmmp67	PROPN
ajpf-645	15	21	,	,	PUNCT
ajpf-645	15	22	pmmp68	pmmp68	ADJ
ajpf-645	15	23	and	and	CCONJ
ajpf-645	15	24	pmmp70	pmmp70	NOUN
ajpf-645	15	25	.	.	PUNCT
ajpf-645	16	1	the	the	DET
ajpf-645	16	2	s.	s.	PROPN
ajpf-645	16	3	typhimurium	typhimurium	PROPN
ajpf-645	16	4	strains	strain	VERB
ajpf-645	16	5	harboring	harbor	VERB
ajpf-645	16	6	the	the	DET
ajpf-645	16	7	recombinant	recombinant	ADJ
ajpf-645	16	8	plasmids	plasmid	NOUN
ajpf-645	16	9	expressed	express	VERB
ajpf-645	16	10	the	the	DET
ajpf-645	16	11	his6	his6	NOUN
ajpf-645	16	12	-	-	PUNCT
ajpf-645	16	13	tagged	tag	VERB
ajpf-645	16	14	pspa	pspa	NOUN
ajpf-645	16	15	,	,	PUNCT
ajpf-645	16	16	demonstrating	demonstrate	VERB
ajpf-645	16	17	in	in	ADP
ajpf-645	16	18	-	-	PUNCT
ajpf-645	16	19	frame	frame	NOUN
ajpf-645	16	20	fusion	fusion	NOUN
ajpf-645	16	21	of	of	ADP
ajpf-645	16	22	pspa	pspa	NOUN
ajpf-645	16	23	to	to	ADP
ajpf-645	16	24	each	each	PRON
ajpf-645	16	25	depending	depend	VERB
ajpf-645	16	26	upon	upon	SCONJ
ajpf-645	16	27	the	the	DET
ajpf-645	16	28	type	type	NOUN
ajpf-645	16	29	of	of	ADP
ajpf-645	16	30	ss	ss	NOUN
ajpf-645	16	31	,	,	PUNCT
ajpf-645	16	32	cell	cell	NOUN
ajpf-645	16	33	lysate	lysate	NOUN
ajpf-645	16	34	as	as	ADV
ajpf-645	16	35	well	well	ADV
ajpf-645	16	36	as	as	ADP
ajpf-645	16	37	secreted	secrete	VERB
ajpf-645	16	38	pspa	pspa	NOUN
ajpf-645	16	39	of	of	ADP
ajpf-645	16	40	each	each	DET
ajpf-645	16	41	salmonella	salmonella	NOUN
ajpf-645	16	42	samples	sample	NOUN
ajpf-645	16	43	was	be	AUX
ajpf-645	16	44	found	find	VERB
ajpf-645	16	45	different	different	ADJ
ajpf-645	16	46	.	.	PUNCT
ajpf-645	17	1	relatively	relatively	ADV
ajpf-645	17	2	,	,	PUNCT
ajpf-645	17	3	s.	s.	PROPN
ajpf-645	17	4	typhimurium	typhimurium	NOUN
ajpf-645	17	5	containing	contain	VERB
ajpf-645	17	6	pmmp67	pmmp67	NOUN
ajpf-645	17	7	(	(	PUNCT
ajpf-645	17	8	carrying	carry	VERB
ajpf-645	17	9	agfa	agfa	PROPN
ajpf-645	17	10	ss	ss	PROPN
ajpf-645	17	11	)	)	PUNCT
ajpf-645	17	12	secreted	secrete	VERB
ajpf-645	17	13	the	the	DET
ajpf-645	17	14	highest	high	ADJ
ajpf-645	17	15	level	level	NOUN
ajpf-645	17	16	of	of	ADP
ajpf-645	17	17	pspa	pspa	NOUN
ajpf-645	17	18	than	than	ADP
ajpf-645	17	19	others	other	NOUN
ajpf-645	17	20	,	,	PUNCT
ajpf-645	17	21	and	and	CCONJ
ajpf-645	17	22	suggested	suggest	VERB
ajpf-645	17	23	that	that	SCONJ
ajpf-645	17	24	the	the	DET
ajpf-645	17	25	agfa	agfa	PROPN
ajpf-645	17	26	ss	ss	PROPN
ajpf-645	17	27	mediates	mediates	PROPN
ajpf-645	17	28	efficient	efficient	ADJ
ajpf-645	17	29	translocation	translocation	NOUN
ajpf-645	17	30	of	of	ADP
ajpf-645	17	31	the	the	DET
ajpf-645	17	32	pspa	pspa	NOUN
ajpf-645	17	33	.	.	PUNCT
ajpf-645	18	1	conclusively	conclusively	ADV
ajpf-645	18	2	,	,	PUNCT
ajpf-645	18	3	the	the	DET
ajpf-645	18	4	agfa	agfa	PROPN
ajpf-645	18	5	ss	ss	PROPN
ajpf-645	18	6	mediated	mediate	VERB
ajpf-645	18	7	secretion	secretion	NOUN
ajpf-645	18	8	system	system	NOUN
ajpf-645	18	9	in	in	ADP
ajpf-645	18	10	pmmp67	pmmp67	NOUN
ajpf-645	18	11	can	can	AUX
ajpf-645	18	12	be	be	AUX
ajpf-645	18	13	used	use	VERB
ajpf-645	18	14	in	in	ADP
ajpf-645	18	15	variety	variety	NOUN
ajpf-645	18	16	fields	field	NOUN
ajpf-645	18	17	required	require	VERB
ajpf-645	18	18	for	for	ADP
ajpf-645	18	19	the	the	DET
ajpf-645	18	20	high	high	ADJ
ajpf-645	18	21	level	level	NOUN
ajpf-645	18	22	of	of	ADP
ajpf-645	18	23	protein	protein	NOUN
ajpf-645	18	24	secretion	secretion	NOUN
ajpf-645	18	25	,	,	PUNCT
ajpf-645	18	26	especially	especially	ADV
ajpf-645	18	27	antigen	antigen	ADJ
ajpf-645	18	28	delivery	delivery	NOUN
ajpf-645	18	29	in	in	ADP
ajpf-645	18	30	recombinant	recombinant	ADJ
ajpf-645	18	31	attenuated	attenuate	VERB
ajpf-645	18	32	salmonella	salmonella	NOUN
ajpf-645	18	33	vaccines	vaccine	NOUN
ajpf-645	18	34	.	.	PUNCT
ajpf-645	19	1	key	key	ADJ
ajpf-645	19	2	words	word	NOUN
ajpf-645	19	3	:	:	PUNCT
ajpf-645	19	4	–lactamase	–lactamase	ADJ
ajpf-645	19	5	,	,	PUNCT
ajpf-645	19	6	thin	thin	ADJ
ajpf-645	19	7	aggregative	aggregative	ADJ
ajpf-645	19	8	fimbriae	fimbriae	NOUN
ajpf-645	19	9	a	a	PRON
ajpf-645	19	10	,	,	PUNCT
ajpf-645	19	11	outer	outer	ADJ
ajpf-645	19	12	membrane	membrane	NOUN
ajpf-645	19	13	protein	protein	NOUN
ajpf-645	19	14	w	w	NOUN
ajpf-645	19	15	,	,	PUNCT
ajpf-645	19	16	salmonella	salmonella	NOUN
ajpf-645	19	17	typhimurium	typhimurium	DET
ajpf-645	19	18	fimbriae	fimbriae	NOUN
ajpf-645	19	19	a	a	DET
ajpf-645	19	20	,	,	PUNCT
ajpf-645	19	21	signal	signal	ADJ
ajpf-645	19	22	sequence	sequence	NOUN
ajpf-645	19	23	,	,	PUNCT
ajpf-645	19	24	secretion	secretion	NOUN
ajpf-645	19	25	.	.	PUNCT
ajpf-645	20	1	introduction	introduction	NOUN
ajpf-645	20	2	the	the	DET
ajpf-645	20	3	conventional	conventional	ADJ
ajpf-645	20	4	vaccines	vaccine	NOUN
ajpf-645	20	5	against	against	ADP
ajpf-645	20	6	pathogenic	pathogenic	ADJ
ajpf-645	20	7	bacteria	bacteria	NOUN
ajpf-645	20	8	have	have	AUX
ajpf-645	20	9	been	be	AUX
ajpf-645	20	10	produced	produce	VERB
ajpf-645	20	11	to	to	PART
ajpf-645	20	12	majorly	majorly	ADV
ajpf-645	20	13	kill	kill	VERB
ajpf-645	20	14	these	these	DET
ajpf-645	20	15	bacteria	bacteria	NOUN
ajpf-645	20	16	or	or	CCONJ
ajpf-645	20	17	act	act	VERB
ajpf-645	20	18	*	*	PUNCT
ajpf-645	20	19	corresponding	correspond	VERB
ajpf-645	20	20	author	author	NOUN
ajpf-645	20	21	.	.	PUNCT
ajpf-645	21	1	e	e	X
ajpf-645	21	2	-	-	NOUN
ajpf-645	21	3	mail	mail	NOUN
ajpf-645	21	4	:	:	PUNCT
ajpf-645	21	5	swkim@gntech.ac.kr	swkim@gntech.ac.kr	NOUN
ajpf-645	21	6	.	.	NOUN
ajpf-645	21	7	tel	tel	ADP
ajpf-645	21	8	:	:	PUNCT
ajpf-645	21	9	82	82	NUM
ajpf-645	21	10	55	55	NUM
ajpf-645	21	11	-	-	PUNCT
ajpf-645	21	12	751	751	NUM
ajpf-645	21	13	-	-	NUM
ajpf-645	21	14	3268	3268	NUM
ajpf-645	21	15	.	.	PUNCT
ajpf-645	22	1	fax	fax	NOUN
ajpf-645	22	2	:	:	PUNCT
ajpf-645	22	3	82	82	NUM
ajpf-645	22	4	-	-	SYM
ajpf-645	22	5	55	55	NUM
ajpf-645	22	6	-	-	PUNCT
ajpf-645	22	7	751	751	NUM
ajpf-645	22	8	-	-	PUNCT
ajpf-645	22	9	3280	3280	NUM
ajpf-645	22	10	.	.	PUNCT
ajpf-645	23	1	as	as	ADP
ajpf-645	23	2	subunit	subunit	NOUN
ajpf-645	23	3	vaccines	vaccine	NOUN
ajpf-645	23	4	(	(	PUNCT
ajpf-645	23	5	meeusen	meeusen	NOUN
ajpf-645	23	6	et	et	PROPN
ajpf-645	23	7	al	al	PROPN
ajpf-645	23	8	.	.	PROPN
ajpf-645	23	9	,	,	PUNCT
ajpf-645	23	10	2007	2007	NUM
ajpf-645	23	11	)	)	PUNCT
ajpf-645	23	12	.	.	PUNCT
ajpf-645	24	1	however	however	ADV
ajpf-645	24	2	,	,	PUNCT
ajpf-645	24	3	the	the	DET
ajpf-645	24	4	manufacturing	manufacturing	NOUN
ajpf-645	24	5	of	of	ADP
ajpf-645	24	6	these	these	DET
ajpf-645	24	7	vaccines	vaccine	NOUN
ajpf-645	24	8	are	be	AUX
ajpf-645	24	9	very	very	ADV
ajpf-645	24	10	complicated	complicated	ADJ
ajpf-645	24	11	from	from	ADP
ajpf-645	24	12	isolation	isolation	NOUN
ajpf-645	24	13	up	up	ADP
ajpf-645	24	14	to	to	ADP
ajpf-645	24	15	the	the	DET
ajpf-645	24	16	final	final	ADJ
ajpf-645	24	17	step	step	NOUN
ajpf-645	24	18	.	.	PUNCT
ajpf-645	25	1	additionally	additionally	ADV
ajpf-645	25	2	,	,	PUNCT
ajpf-645	25	3	the	the	DET
ajpf-645	25	4	prepared	prepare	VERB
ajpf-645	25	5	vaccines	vaccine	NOUN
ajpf-645	25	6	require	require	VERB
ajpf-645	25	7	stable	stable	ADJ
ajpf-645	25	8	storage	storage	NOUN
ajpf-645	25	9	to	to	PART
ajpf-645	25	10	avoid	avoid	VERB
ajpf-645	25	11	any	any	DET
ajpf-645	25	12	side	side	NOUN
ajpf-645	25	13	effects	effect	NOUN
ajpf-645	25	14	or	or	CCONJ
ajpf-645	25	15	denaturation	denaturation	NOUN
ajpf-645	25	16	.	.	PUNCT
ajpf-645	26	1	these	these	DET
ajpf-645	26	2	vaccines	vaccine	NOUN
ajpf-645	26	3	are	be	AUX
ajpf-645	26	4	most	most	ADV
ajpf-645	26	5	likely	likely	ADV
ajpf-645	26	6	administrated	administrate	VERB
ajpf-645	26	7	to	to	PART
ajpf-645	26	8	host	host	VERB
ajpf-645	26	9	through	through	ADP
ajpf-645	26	10	syringe	syringe	NOUN
ajpf-645	26	11	,	,	PUNCT
ajpf-645	26	12	thereby	thereby	ADV
ajpf-645	26	13	making	make	VERB
ajpf-645	26	14	the	the	DET
ajpf-645	26	15	child	child	NOUN
ajpf-645	26	16	to	to	PART
ajpf-645	26	17	be	be	AUX
ajpf-645	26	18	afraid	afraid	ADJ
ajpf-645	26	19	.	.	PUNCT
ajpf-645	27	1	one	one	NUM
ajpf-645	27	2	way	way	NOUN
ajpf-645	27	3	to	to	PART
ajpf-645	27	4	resolve	resolve	VERB
ajpf-645	27	5	these	these	DET
ajpf-645	27	6	table	table	NOUN
ajpf-645	27	7	1	1	NUM
ajpf-645	27	8	.	.	PUNCT
ajpf-645	27	9	bacterial	bacterial	ADJ
ajpf-645	27	10	strains	strain	NOUN
ajpf-645	27	11	and	and	CCONJ
ajpf-645	27	12	plasmids	plasmid	NOUN
ajpf-645	27	13	used	use	VERB
ajpf-645	27	14	for	for	ADP
ajpf-645	27	15	this	this	DET
ajpf-645	27	16	study	study	NOUN
ajpf-645	27	17	.	.	PUNCT
ajpf-645	28	1	strain	strain	VERB
ajpf-645	28	2	or	or	CCONJ
ajpf-645	28	3	plasmid	plasmid	ADJ
ajpf-645	28	4	genotype	genotype	NOUN
ajpf-645	28	5	or	or	CCONJ
ajpf-645	28	6	phenotype	phenotype	NOUN
ajpf-645	28	7	reference	reference	NOUN
ajpf-645	28	8	or	or	CCONJ
ajpf-645	28	9	source	source	NOUN
ajpf-645	28	10	bacterial	bacterial	ADJ
ajpf-645	28	11	strains	strain	NOUN
ajpf-645	28	12	e.	e.	NOUN
ajpf-645	28	13	coli	coli	NOUN
ajpf-645	28	14	dh5	dh5	ADJ
ajpf-645	28	15	enda1	enda1	PROPN
ajpf-645	28	16	hsdr17	hsdr17	PROPN
ajpf-645	28	17	supe44	supe44	PROPN
ajpf-645	28	18	thi-1	thi-1	PUNCT
ajpf-645	28	19	reca1	reca1	NOUN
ajpf-645	28	20	gyra	gyra	NOUN
ajpf-645	28	21	rela1	rela1	NOUN
ajpf-645	28	22	(	(	PUNCT
ajpf-645	28	23	laczya	laczya	NOUN
ajpf-645	28	24	-	-	PUNCT
ajpf-645	28	25	argf)u169	argf)u169	NOUN
ajpf-645	28	26	(	(	PUNCT
ajpf-645	28	27	80lacz	80lacz	NUM
ajpf-645	28	28	m15	m15	NUM
ajpf-645	28	29	)	)	PUNCT
ajpf-645	28	30	new	new	PROPN
ajpf-645	28	31	england	england	PROPN
ajpf-645	28	32	biolabs	biolab	VERB
ajpf-645	28	33	6212	6212	NUM
ajpf-645	28	34	f	f	SYM
ajpf-645	28	35	80	80	NUM
ajpf-645	28	36	(	(	PUNCT
ajpf-645	28	37	laczya	laczya	NOUN
ajpf-645	28	38	-	-	PUNCT
ajpf-645	28	39	argf)enda1reca1hadr17deorthi-1glnv44gyra96	argf)enda1reca1hadr17deorthi-1glnv44gyra96	ADJ
ajpf-645	28	40	rela1	rela1	NOUN
ajpf-645	28	41	asda4	asda4	NOUN
ajpf-645	28	42	nakayama	nakayama	PROPN
ajpf-645	28	43	et	et	PROPN
ajpf-645	28	44	al	al	PROPN
ajpf-645	28	45	.	.	PROPN
ajpf-645	29	1	(	(	PUNCT
ajpf-645	29	2	1988	1988	NUM
ajpf-645	29	3	)	)	PUNCT
ajpf-645	29	4	salmonella	salmonella	NOUN
ajpf-645	29	5	8554	8554	NUM
ajpf-645	29	6	hisg	hisg	PROPN
ajpf-645	29	7	asda16	asda16	PROPN
ajpf-645	29	8	kang	kang	PROPN
ajpf-645	29	9	et	et	PROPN
ajpf-645	29	10	al	al	PROPN
ajpf-645	29	11	.	.	PROPN
ajpf-645	30	1	(	(	PUNCT
ajpf-645	30	2	2002	2002	NUM
ajpf-645	30	3	)	)	PUNCT
ajpf-645	30	4	plasmids	plasmid	NOUN
ajpf-645	30	5	pgem	pgem	NOUN
ajpf-645	30	6	-	-	PUNCT
ajpf-645	30	7	t	t	NOUN
ajpf-645	30	8	vector	vector	NOUN
ajpf-645	30	9	cloning	cloning	NOUN
ajpf-645	30	10	vector	vector	NOUN
ajpf-645	30	11	promega	promega	PROPN
ajpf-645	30	12	pya3342	pya3342	PROPN
ajpf-645	30	13	asd	asd	PROPN
ajpf-645	30	14	pbrori	pbrori	PROPN
ajpf-645	30	15	kang	kang	PROPN
ajpf-645	30	16	et	et	PROPN
ajpf-645	30	17	al	al	PROPN
ajpf-645	30	18	.	.	PROPN
ajpf-645	31	1	(	(	PUNCT
ajpf-645	31	2	2002	2002	NUM
ajpf-645	31	3	)	)	PUNCT
ajpf-645	31	4	pya3493	pya3493	ADJ
ajpf-645	31	5	derivative	derivative	ADJ
ajpf-645	31	6	-lactamase	-lactamase	NOUN
ajpf-645	31	7	signal	signal	NOUN
ajpf-645	31	8	sequence	sequence	NOUN
ajpf-645	31	9	-	-	PUNCT
ajpf-645	31	10	based	base	VERB
ajpf-645	31	11	periplasmmic	periplasmmic	ADJ
ajpf-645	31	12	secretion	secretion	NOUN
ajpf-645	31	13	plasmid	plasmid	NOUN
ajpf-645	31	14	kang	kang	PROPN
ajpf-645	31	15	et	et	PROPN
ajpf-645	31	16	al	al	PROPN
ajpf-645	31	17	.	.	PROPN
ajpf-645	32	1	(	(	PUNCT
ajpf-645	32	2	2002	2002	NUM
ajpf-645	32	3	)	)	PUNCT
ajpf-645	32	4	pya3494	pya3494	VERB
ajpf-645	32	5	a	a	DET
ajpf-645	32	6	pya3493	pya3493	ADJ
ajpf-645	32	7	derivative	derivative	NOUN
ajpf-645	32	8	containing	contain	VERB
ajpf-645	32	9	pspa	pspa	PROPN
ajpf-645	32	10	gene	gene	NOUN
ajpf-645	32	11	kang	kang	PROPN
ajpf-645	32	12	et	et	PROPN
ajpf-645	32	13	al	al	PROPN
ajpf-645	32	14	.	.	PROPN
ajpf-645	33	1	(	(	PUNCT
ajpf-645	33	2	2002	2002	NUM
ajpf-645	33	3	)	)	PUNCT
ajpf-645	33	4	pmmp58	pmmp58	NOUN
ajpf-645	33	5	a	a	DET
ajpf-645	33	6	derivative	derivative	NOUN
ajpf-645	33	7	of	of	ADP
ajpf-645	33	8	t	t	PROPN
ajpf-645	33	9	vector	vector	NOUN
ajpf-645	33	10	containing	contain	VERB
ajpf-645	33	11	bla	bla	NOUN
ajpf-645	33	12	signal	signal	NOUN
ajpf-645	33	13	sequence	sequence	NOUN
ajpf-645	33	14	(	(	PUNCT
ajpf-645	33	15	ss	ss	NOUN
ajpf-645	33	16	)	)	PUNCT
ajpf-645	33	17	and	and	CCONJ
ajpf-645	33	18	his	his	PRON
ajpf-645	33	19	-	-	PUNCT
ajpf-645	33	20	tag	tag	NOUN
ajpf-645	33	21	this	this	DET
ajpf-645	33	22	study	study	NOUN
ajpf-645	33	23	pmmp59	pmmp59	NOUN
ajpf-645	33	24	a	a	DET
ajpf-645	33	25	derivative	derivative	NOUN
ajpf-645	33	26	of	of	ADP
ajpf-645	33	27	t	t	PROPN
ajpf-645	33	28	vector	vector	NOUN
ajpf-645	33	29	containing	contain	VERB
ajpf-645	33	30	agfa	agfa	PROPN
ajpf-645	33	31	ss	ss	PROPN
ajpf-645	33	32	and	and	CCONJ
ajpf-645	33	33	his	his	PRON
ajpf-645	33	34	-	-	PUNCT
ajpf-645	33	35	tag	tag	NOUN
ajpf-645	33	36	this	this	DET
ajpf-645	33	37	study	study	NOUN
ajpf-645	33	38	pmmp60	pmmp60	NOUN
ajpf-645	33	39	a	a	DET
ajpf-645	33	40	derivative	derivative	NOUN
ajpf-645	33	41	of	of	ADP
ajpf-645	33	42	t	t	PROPN
ajpf-645	33	43	vector	vector	NOUN
ajpf-645	33	44	containing	contain	VERB
ajpf-645	33	45	ompw	ompw	NOUN
ajpf-645	33	46	ss	ss	PROPN
ajpf-645	33	47	and	and	CCONJ
ajpf-645	33	48	his	his	PRON
ajpf-645	33	49	-	-	PUNCT
ajpf-645	33	50	tag	tag	NOUN
ajpf-645	33	51	this	this	DET
ajpf-645	33	52	study	study	NOUN
ajpf-645	33	53	pmmp61	pmmp61	VERB
ajpf-645	33	54	a	a	DET
ajpf-645	33	55	derivative	derivative	NOUN
ajpf-645	33	56	of	of	ADP
ajpf-645	33	57	t	t	PROPN
ajpf-645	33	58	vector	vector	NOUN
ajpf-645	33	59	containing	contain	VERB
ajpf-645	33	60	stfa	stfa	NOUN
ajpf-645	33	61	ss	ss	PROPN
ajpf-645	33	62	and	and	CCONJ
ajpf-645	33	63	his	his	PRON
ajpf-645	33	64	-	-	PUNCT
ajpf-645	33	65	tag	tag	NOUN
ajpf-645	33	66	this	this	DET
ajpf-645	33	67	study	study	NOUN
ajpf-645	33	68	pmmp62	pmmp62	NOUN
ajpf-645	33	69	a	a	DET
ajpf-645	33	70	derivative	derivative	NOUN
ajpf-645	33	71	of	of	ADP
ajpf-645	33	72	pya3342	pya3342	NOUN
ajpf-645	33	73	containing	contain	VERB
ajpf-645	33	74	ompw	ompw	NOUN
ajpf-645	33	75	ss	ss	PROPN
ajpf-645	33	76	and	and	CCONJ
ajpf-645	33	77	his	his	PRON
ajpf-645	33	78	-	-	PUNCT
ajpf-645	33	79	tag	tag	NOUN
ajpf-645	33	80	this	this	DET
ajpf-645	33	81	study	study	NOUN
ajpf-645	33	82	pmmp63	pmmp63	ADV
ajpf-645	33	83	a	a	DET
ajpf-645	33	84	derivative	derivative	NOUN
ajpf-645	33	85	of	of	ADP
ajpf-645	33	86	pya3342	pya3342	NOUN
ajpf-645	33	87	containing	contain	VERB
ajpf-645	33	88	stfa	stfa	PROPN
ajpf-645	33	89	ss	ss	PROPN
ajpf-645	33	90	and	and	CCONJ
ajpf-645	33	91	his	his	PRON
ajpf-645	33	92	-	-	PUNCT
ajpf-645	33	93	tag	tag	NOUN
ajpf-645	33	94	this	this	DET
ajpf-645	33	95	study	study	NOUN
ajpf-645	33	96	pmmp64	pmmp64	NOUN
ajpf-645	33	97	a	a	DET
ajpf-645	33	98	derivative	derivative	NOUN
ajpf-645	33	99	of	of	ADP
ajpf-645	33	100	pya3342	pya3342	NOUN
ajpf-645	33	101	containing	contain	VERB
ajpf-645	33	102	bla	bla	NOUN
ajpf-645	33	103	ss	ss	PROPN
ajpf-645	33	104	and	and	CCONJ
ajpf-645	33	105	his	his	PRON
ajpf-645	33	106	-	-	PUNCT
ajpf-645	33	107	tag	tag	NOUN
ajpf-645	33	108	this	this	DET
ajpf-645	33	109	study	study	NOUN
ajpf-645	33	110	pmmp65	pmmp65	VERB
ajpf-645	33	111	a	a	DET
ajpf-645	33	112	derivative	derivative	NOUN
ajpf-645	33	113	of	of	ADP
ajpf-645	33	114	pya3342	pya3342	NOUN
ajpf-645	33	115	containing	contain	VERB
ajpf-645	33	116	agfa	agfa	PROPN
ajpf-645	33	117	ss	ss	PROPN
ajpf-645	33	118	and	and	CCONJ
ajpf-645	33	119	his	his	PRON
ajpf-645	33	120	-	-	PUNCT
ajpf-645	33	121	tag	tag	NOUN
ajpf-645	33	122	this	this	DET
ajpf-645	33	123	study	study	NOUN
ajpf-645	33	124	pmmp66	pmmp66	NOUN
ajpf-645	33	125	a	a	DET
ajpf-645	33	126	derivative	derivative	NOUN
ajpf-645	33	127	of	of	ADP
ajpf-645	33	128	pmmp63	pmmp63	NOUN
ajpf-645	33	129	containing	contain	VERB
ajpf-645	33	130	pspa	pspa	ADJ
ajpf-645	33	131	gene	gene	NOUN
ajpf-645	33	132	this	this	DET
ajpf-645	33	133	study	study	NOUN
ajpf-645	33	134	pmmp67	pmmp67	VERB
ajpf-645	33	135	a	a	DET
ajpf-645	33	136	derivative	derivative	NOUN
ajpf-645	33	137	of	of	ADP
ajpf-645	33	138	pmmp65	pmmp65	NOUN
ajpf-645	33	139	containing	contain	VERB
ajpf-645	33	140	pspa	pspa	ADJ
ajpf-645	33	141	gene	gene	NOUN
ajpf-645	33	142	this	this	DET
ajpf-645	33	143	study	study	NOUN
ajpf-645	33	144	pmmp68	pmmp68	VERB
ajpf-645	33	145	a	a	DET
ajpf-645	33	146	derivative	derivative	NOUN
ajpf-645	33	147	of	of	ADP
ajpf-645	33	148	pmmp64	pmmp64	NOUN
ajpf-645	33	149	containing	contain	VERB
ajpf-645	33	150	pspa	pspa	ADJ
ajpf-645	33	151	gene	gene	NOUN
ajpf-645	33	152	this	this	DET
ajpf-645	33	153	study	study	NOUN
ajpf-645	33	154	pmmp70	pmmp70	VERB
ajpf-645	33	155	a	a	DET
ajpf-645	33	156	derivative	derivative	NOUN
ajpf-645	33	157	of	of	ADP
ajpf-645	33	158	pmmp62	pmmp62	NOUN
ajpf-645	33	159	containing	contain	VERB
ajpf-645	33	160	pspa	pspa	ADJ
ajpf-645	33	161	gene	gene	NOUN
ajpf-645	33	162	this	this	DET
ajpf-645	33	163	study	study	NOUN
ajpf-645	33	164	inconveniences	inconvenience	NOUN
ajpf-645	33	165	is	be	AUX
ajpf-645	33	166	the	the	DET
ajpf-645	33	167	development	development	NOUN
ajpf-645	33	168	of	of	ADP
ajpf-645	33	169	attenuated	attenuate	VERB
ajpf-645	33	170	bacterial	bacterial	ADJ
ajpf-645	33	171	vaccines	vaccine	NOUN
ajpf-645	33	172	.	.	PUNCT
ajpf-645	34	1	recombinant	recombinant	ADJ
ajpf-645	34	2	attenuated	attenuate	VERB
ajpf-645	34	3	salmonella	salmonella	NOUN
ajpf-645	34	4	vaccines	vaccine	NOUN
ajpf-645	34	5	have	have	AUX
ajpf-645	34	6	been	be	AUX
ajpf-645	34	7	adapted	adapt	VERB
ajpf-645	34	8	to	to	PART
ajpf-645	34	9	elicit	elicit	VERB
ajpf-645	34	10	mucosal	mucosal	NOUN
ajpf-645	34	11	and	and	CCONJ
ajpf-645	34	12	humoral	humoral	ADJ
ajpf-645	34	13	immunities	immunity	NOUN
ajpf-645	34	14	after	after	ADP
ajpf-645	34	15	oral	oral	ADJ
ajpf-645	34	16	administration	administration	NOUN
ajpf-645	34	17	.	.	PUNCT
ajpf-645	35	1	the	the	DET
ajpf-645	35	2	elicited	elicit	VERB
ajpf-645	35	3	immune	immune	ADJ
ajpf-645	35	4	responses	response	NOUN
ajpf-645	35	5	were	be	AUX
ajpf-645	35	6	augmented	augment	VERB
ajpf-645	35	7	when	when	SCONJ
ajpf-645	35	8	the	the	DET
ajpf-645	35	9	delivered	deliver	VERB
ajpf-645	35	10	antigen	antigen	NOUN
ajpf-645	35	11	was	be	AUX
ajpf-645	35	12	translocated	translocate	VERB
ajpf-645	35	13	into	into	ADP
ajpf-645	35	14	cell	cell	NOUN
ajpf-645	35	15	envelop	envelop	NOUN
ajpf-645	35	16	or	or	CCONJ
ajpf-645	35	17	out	out	ADP
ajpf-645	35	18	of	of	ADP
ajpf-645	35	19	the	the	DET
ajpf-645	35	20	cells	cell	NOUN
ajpf-645	35	21	(	(	PUNCT
ajpf-645	35	22	kang	kang	PROPN
ajpf-645	35	23	and	and	CCONJ
ajpf-645	35	24	curtiss	curtiss	PROPN
ajpf-645	35	25	,	,	PUNCT
ajpf-645	35	26	2003	2003	NUM
ajpf-645	35	27	;	;	PUNCT
ajpf-645	35	28	kang	kang	PROPN
ajpf-645	35	29	et	et	PROPN
ajpf-645	35	30	al	al	PROPN
ajpf-645	35	31	.	.	PROPN
ajpf-645	35	32	,	,	PUNCT
ajpf-645	35	33	2002	2002	NUM
ajpf-645	35	34	;	;	PUNCT
ajpf-645	35	35	kim	kim	PROPN
ajpf-645	35	36	et	et	PROPN
ajpf-645	35	37	al	al	PROPN
ajpf-645	35	38	.	.	PROPN
ajpf-645	35	39	,	,	PUNCT
ajpf-645	35	40	2007	2007	NUM
ajpf-645	35	41	)	)	PUNCT
ajpf-645	35	42	.	.	PUNCT
ajpf-645	36	1	multiple	multiple	ADJ
ajpf-645	36	2	methods	method	NOUN
ajpf-645	36	3	were	be	AUX
ajpf-645	36	4	applied	apply	VERB
ajpf-645	36	5	to	to	PART
ajpf-645	36	6	translocate	translocate	VERB
ajpf-645	36	7	the	the	DET
ajpf-645	36	8	foreign	foreign	ADJ
ajpf-645	36	9	antigen	antigen	NOUN
ajpf-645	36	10	from	from	ADP
ajpf-645	36	11	cytosol	cytosol	NOUN
ajpf-645	36	12	into	into	ADP
ajpf-645	36	13	at	at	ADP
ajpf-645	36	14	least	least	ADJ
ajpf-645	36	15	cell	cell	NOUN
ajpf-645	36	16	envelop	envelop	VERB
ajpf-645	36	17	or	or	CCONJ
ajpf-645	36	18	complete	complete	ADJ
ajpf-645	36	19	secretion	secretion	NOUN
ajpf-645	36	20	(	(	PUNCT
ajpf-645	36	21	kim	kim	PROPN
ajpf-645	36	22	et	et	PROPN
ajpf-645	36	23	al	al	PROPN
ajpf-645	36	24	.	.	PROPN
ajpf-645	36	25	,	,	PUNCT
ajpf-645	36	26	2007	2007	NUM
ajpf-645	36	27	;	;	PUNCT
ajpf-645	36	28	wong	wong	PROPN
ajpf-645	36	29	et	et	PROPN
ajpf-645	36	30	al	al	PROPN
ajpf-645	36	31	.	.	PROPN
ajpf-645	36	32	,	,	PUNCT
ajpf-645	36	33	1995	1995	NUM
ajpf-645	36	34	)	)	PUNCT
ajpf-645	36	35	.	.	PUNCT
ajpf-645	37	1	researchers	researcher	NOUN
ajpf-645	37	2	inserted	insert	VERB
ajpf-645	37	3	the	the	DET
ajpf-645	37	4	target	target	NOUN
ajpf-645	37	5	antigen	antigen	ADV
ajpf-645	37	6	or	or	CCONJ
ajpf-645	37	7	epitope	epitope	NOUN
ajpf-645	37	8	into	into	ADP
ajpf-645	37	9	surface	surface	NOUN
ajpf-645	37	10	structure	structure	NOUN
ajpf-645	37	11	molecules	molecule	NOUN
ajpf-645	37	12	such	such	ADJ
ajpf-645	37	13	as	as	ADP
ajpf-645	37	14	flagella	flagella	NOUN
ajpf-645	37	15	or	or	CCONJ
ajpf-645	37	16	fimbriae	fimbriae	NOUN
ajpf-645	37	17	to	to	PART
ajpf-645	37	18	present	present	VERB
ajpf-645	37	19	the	the	DET
ajpf-645	37	20	antigen	antigen	NOUN
ajpf-645	37	21	on	on	ADP
ajpf-645	37	22	cell	cell	NOUN
ajpf-645	37	23	surface	surface	NOUN
ajpf-645	37	24	(	(	PUNCT
ajpf-645	37	25	majander	majander	NOUN
ajpf-645	37	26	et	et	PROPN
ajpf-645	37	27	al	al	PROPN
ajpf-645	37	28	.	.	PROPN
ajpf-645	37	29	,	,	PUNCT
ajpf-645	37	30	2005a	2005a	NUM
ajpf-645	37	31	,	,	PUNCT
ajpf-645	37	32	b	b	X
ajpf-645	37	33	;	;	PUNCT
ajpf-645	37	34	spreng	spreng	PROPN
ajpf-645	37	35	et	et	PROPN
ajpf-645	37	36	al	al	PROPN
ajpf-645	37	37	.	.	PROPN
ajpf-645	37	38	,	,	PUNCT
ajpf-645	37	39	1999	1999	NUM
ajpf-645	37	40	)	)	PUNCT
ajpf-645	37	41	.	.	PUNCT
ajpf-645	38	1	however	however	ADV
ajpf-645	38	2	,	,	PUNCT
ajpf-645	38	3	a	a	DET
ajpf-645	38	4	limited	limited	ADJ
ajpf-645	38	5	size	size	NOUN
ajpf-645	38	6	of	of	ADP
ajpf-645	38	7	the	the	DET
ajpf-645	38	8	antigen	antigen	NOUN
ajpf-645	38	9	can	can	AUX
ajpf-645	38	10	be	be	AUX
ajpf-645	38	11	inserted	insert	VERB
ajpf-645	38	12	into	into	ADP
ajpf-645	38	13	surface	surface	NOUN
ajpf-645	38	14	molecule	molecule	NOUN
ajpf-645	38	15	for	for	ADP
ajpf-645	38	16	stable	stable	ADJ
ajpf-645	38	17	conformation	conformation	NOUN
ajpf-645	38	18	.	.	PUNCT
ajpf-645	39	1	another	another	DET
ajpf-645	39	2	more	more	ADV
ajpf-645	39	3	convenient	convenient	ADJ
ajpf-645	39	4	way	way	NOUN
ajpf-645	39	5	is	be	AUX
ajpf-645	39	6	the	the	DET
ajpf-645	39	7	secretion	secretion	NOUN
ajpf-645	39	8	of	of	ADP
ajpf-645	39	9	the	the	DET
ajpf-645	39	10	foreign	foreign	ADJ
ajpf-645	39	11	protein	protein	NOUN
ajpf-645	39	12	into	into	ADP
ajpf-645	39	13	extracellular	extracellular	ADJ
ajpf-645	39	14	matrix	matrix	NOUN
ajpf-645	39	15	through	through	ADP
ajpf-645	39	16	the	the	DET
ajpf-645	39	17	use	use	NOUN
ajpf-645	39	18	of	of	ADP
ajpf-645	39	19	a	a	DET
ajpf-645	39	20	signal	signal	ADJ
ajpf-645	39	21	sequence	sequence	NOUN
ajpf-645	39	22	(	(	PUNCT
ajpf-645	39	23	ss	ss	NOUN
ajpf-645	39	24	)	)	PUNCT
ajpf-645	39	25	(	(	PUNCT
ajpf-645	39	26	koshland	koshland	NOUN
ajpf-645	39	27	and	and	CCONJ
ajpf-645	39	28	botstein	botstein	ADJ
ajpf-645	39	29	,	,	PUNCT
ajpf-645	39	30	1980	1980	NUM
ajpf-645	39	31	;	;	PUNCT
ajpf-645	39	32	li	li	PROPN
ajpf-645	39	33	et	et	PROPN
ajpf-645	39	34	al	al	PROPN
ajpf-645	39	35	.	.	PROPN
ajpf-645	39	36	,	,	PUNCT
ajpf-645	39	37	2008	2008	NUM
ajpf-645	39	38	)	)	PUNCT
ajpf-645	39	39	.	.	PUNCT
ajpf-645	40	1	a	a	DET
ajpf-645	40	2	previous	previous	ADJ
ajpf-645	40	3	study	study	NOUN
ajpf-645	40	4	demonstrated	demonstrate	VERB
ajpf-645	40	5	that	that	SCONJ
ajpf-645	40	6	the	the	DET
ajpf-645	40	7	pspa	pspa	NOUN
ajpf-645	40	8	antigen	antigen	NOUN
ajpf-645	40	9	was	be	AUX
ajpf-645	40	10	translocated	translocate	VERB
ajpf-645	40	11	into	into	ADP
ajpf-645	40	12	periplasmic	periplasmic	ADJ
ajpf-645	40	13	space	space	NOUN
ajpf-645	40	14	or	or	CCONJ
ajpf-645	40	15	outside	outside	ADP
ajpf-645	40	16	of	of	ADP
ajpf-645	40	17	the	the	DET
ajpf-645	40	18	cell	cell	NOUN
ajpf-645	40	19	through	through	ADP
ajpf-645	40	20	the	the	DET
ajpf-645	40	21	fusion	fusion	NOUN
ajpf-645	40	22	of	of	ADP
ajpf-645	40	23	-lactamase	-lactamase	NOUN
ajpf-645	40	24	signal	signal	NOUN
ajpf-645	40	25	sequence	sequence	NOUN
ajpf-645	40	26	(	(	PUNCT
ajpf-645	40	27	kang	kang	PROPN
ajpf-645	40	28	et	et	PROPN
ajpf-645	40	29	al	al	PROPN
ajpf-645	40	30	.	.	PROPN
ajpf-645	40	31	,	,	PUNCT
ajpf-645	40	32	2002	2002	NUM
ajpf-645	40	33	)	)	PUNCT
ajpf-645	40	34	.	.	PUNCT
ajpf-645	41	1	although	although	SCONJ
ajpf-645	41	2	this	this	DET
ajpf-645	41	3	system	system	NOUN
ajpf-645	41	4	has	have	VERB
ajpf-645	41	5	a	a	DET
ajpf-645	41	6	merit	merit	NOUN
ajpf-645	41	7	to	to	PART
ajpf-645	41	8	secret	secret	VERB
ajpf-645	41	9	the	the	DET
ajpf-645	41	10	protein	protein	NOUN
ajpf-645	41	11	to	to	ADP
ajpf-645	41	12	the	the	DET
ajpf-645	41	13	extracellular	extracellular	ADJ
ajpf-645	41	14	matrix	matrix	NOUN
ajpf-645	41	15	,	,	PUNCT
ajpf-645	41	16	a	a	DET
ajpf-645	41	17	large	large	ADJ
ajpf-645	41	18	portion	portion	NOUN
ajpf-645	41	19	(	(	PUNCT
ajpf-645	41	20	50	50	NUM
ajpf-645	41	21	%	%	NOUN
ajpf-645	41	22	)	)	PUNCT
ajpf-645	41	23	of	of	ADP
ajpf-645	41	24	the	the	DET
ajpf-645	41	25	expressed	express	VERB
ajpf-645	41	26	proteins	protein	NOUN
ajpf-645	41	27	exists	exist	VERB
ajpf-645	41	28	still	still	ADV
ajpf-645	41	29	in	in	ADP
ajpf-645	41	30	the	the	DET
ajpf-645	41	31	cytosol	cytosol	NOUN
ajpf-645	41	32	.	.	PUNCT
ajpf-645	42	1	due	due	ADP
ajpf-645	42	2	to	to	ADP
ajpf-645	42	3	the	the	DET
ajpf-645	42	4	fact	fact	NOUN
ajpf-645	42	5	that	that	SCONJ
ajpf-645	42	6	more	more	ADV
ajpf-645	42	7	secreted	secreted	ADJ
ajpf-645	42	8	antigen	antigen	NOUN
ajpf-645	42	9	elicits	elicit	VERB
ajpf-645	42	10	the	the	DET
ajpf-645	42	11	better	well	ADJ
ajpf-645	42	12	immune	immune	ADJ
ajpf-645	42	13	responses	response	NOUN
ajpf-645	42	14	,	,	PUNCT
ajpf-645	42	15	it	it	PRON
ajpf-645	42	16	is	be	AUX
ajpf-645	42	17	needed	need	VERB
ajpf-645	42	18	to	to	PART
ajpf-645	42	19	develop	develop	VERB
ajpf-645	42	20	a	a	DET
ajpf-645	42	21	new	new	ADJ
ajpf-645	42	22	efficient	efficient	ADJ
ajpf-645	42	23	protein	protein	NOUN
ajpf-645	42	24	secretion	secretion	NOUN
ajpf-645	42	25	system	system	NOUN
ajpf-645	42	26	in	in	ADP
ajpf-645	42	27	live	live	ADJ
ajpf-645	42	28	vaccine	vaccine	NOUN
ajpf-645	42	29	mediated	mediate	VERB
ajpf-645	42	30	antigen	antigen	ADJ
ajpf-645	42	31	delivery	delivery	NOUN
ajpf-645	42	32	.	.	PUNCT
ajpf-645	43	1	this	this	DET
ajpf-645	43	2	study	study	NOUN
ajpf-645	43	3	aimed	aim	VERB
ajpf-645	43	4	to	to	PART
ajpf-645	43	5	develop	develop	VERB
ajpf-645	43	6	an	an	DET
ajpf-645	43	7	efficient	efficient	ADJ
ajpf-645	43	8	protein	protein	NOUN
ajpf-645	43	9	secretion	secretion	NOUN
ajpf-645	43	10	system	system	NOUN
ajpf-645	43	11	in	in	ADP
ajpf-645	43	12	salmonella	salmonella	NOUN
ajpf-645	43	13	.	.	PUNCT
ajpf-645	44	1	materials	material	NOUN
ajpf-645	44	2	and	and	CCONJ
ajpf-645	44	3	methods	method	NOUN
ajpf-645	44	4	bacterial	bacterial	ADJ
ajpf-645	44	5	strains	strain	NOUN
ajpf-645	44	6	and	and	CCONJ
ajpf-645	44	7	agents	agent	NOUN
ajpf-645	44	8	the	the	DET
ajpf-645	44	9	bacterial	bacterial	ADJ
ajpf-645	44	10	strains	strain	NOUN
ajpf-645	44	11	,	,	PUNCT
ajpf-645	44	12	plasmids	plasmid	NOUN
ajpf-645	44	13	and	and	CCONJ
ajpf-645	44	14	oligonucleotides	oligonucleotide	NOUN
ajpf-645	44	15	used	use	VERB
ajpf-645	44	16	for	for	ADP
ajpf-645	44	17	this	this	DET
ajpf-645	44	18	study	study	NOUN
ajpf-645	44	19	are	be	AUX
ajpf-645	44	20	listed	list	VERB
ajpf-645	44	21	in	in	ADP
ajpf-645	44	22	tables	table	NOUN
ajpf-645	44	23	1	1	NUM
ajpf-645	44	24	and	and	CCONJ
ajpf-645	44	25	2	2	NUM
ajpf-645	44	26	.	.	X
ajpf-645	44	27	salmonella	salmonella	PROPN
ajpf-645	44	28	enterica	enterica	PROPN
ajpf-645	44	29	serotype	serotype	PROPN
ajpf-645	44	30	typhimurium	typhimurium	PROPN
ajpf-645	44	31	and	and	CCONJ
ajpf-645	44	32	escherichia	escherichia	NOUN
ajpf-645	44	33	coli	coli	NOUN
ajpf-645	44	34	were	be	AUX
ajpf-645	44	35	grown	grow	VERB
ajpf-645	44	36	at	at	ADP
ajpf-645	44	37	37	37	NUM
ajpf-645	44	38	°	°	ADP
ajpf-645	44	39	c	c	NOUN
ajpf-645	44	40	using	use	VERB
ajpf-645	44	41	luriabertani	luriabertani	PROPN
ajpf-645	44	42	(	(	PUNCT
ajpf-645	44	43	lb	lb	NOUN
ajpf-645	44	44	)	)	PUNCT
ajpf-645	44	45	or	or	CCONJ
ajpf-645	44	46	m9	m9	PROPN
ajpf-645	44	47	minimal	minimal	ADJ
ajpf-645	44	48	medium	medium	NOUN
ajpf-645	44	49	supplemented	supplement	VERB
ajpf-645	44	50	with	with	ADP
ajpf-645	44	51	1.5	1.5	NUM
ajpf-645	44	52	%	%	NOUN
ajpf-645	44	53	agar	agar	NOUN
ajpf-645	44	54	(	(	PUNCT
ajpf-645	44	55	bertani	bertani	PROPN
ajpf-645	44	56	,	,	PUNCT
ajpf-645	44	57	1952	1952	NUM
ajpf-645	44	58	)	)	PUNCT
ajpf-645	44	59	.	.	PUNCT
ajpf-645	45	1	the	the	DET
ajpf-645	45	2	antibiotics	antibiotic	NOUN
ajpf-645	45	3	were	be	AUX
ajpf-645	45	4	added	add	VERB
ajpf-645	45	5	in	in	ADP
ajpf-645	45	6	the	the	DET
ajpf-645	45	7	following	follow	VERB
ajpf-645	45	8	table	table	NOUN
ajpf-645	45	9	2	2	NUM
ajpf-645	45	10	.	.	PUNCT
ajpf-645	46	1	synthetic	synthetic	ADJ
ajpf-645	46	2	oligonucleotides	oligonucleotide	NOUN
ajpf-645	46	3	used	use	VERB
ajpf-645	46	4	for	for	ADP
ajpf-645	46	5	this	this	DET
ajpf-645	46	6	study	study	NOUN
ajpf-645	46	7	.	.	PUNCT
ajpf-645	47	1	oligonucleotide	oligonucleotide	NOUN
ajpf-645	47	2	name	name	NOUN
ajpf-645	47	3	oligonucleotide	oligonucleotide	NOUN
ajpf-645	47	4	sequence	sequence	NOUN
ajpf-645	47	5	(	(	PUNCT
ajpf-645	47	6	5	5	NUM
ajpf-645	47	7	'	'	NUM
ajpf-645	47	8	3	3	NUM
ajpf-645	47	9	'	'	NUM
ajpf-645	47	10	)	)	PUNCT
ajpf-645	47	11	sagfa	sagfa	PROPN
ajpf-645	47	12	-	-	PUNCT
ajpf-645	47	13	f	f	NOUN
ajpf-645	47	14	-	-	PUNCT
ajpf-645	47	15	bsphi	bsphi	NOUN
ajpf-645	47	16	tcatgaaacttttaaaagtggcag	tcatgaaacttttaaaagtggcag	NOUN
ajpf-645	47	17	sagfa	sagfa	PROPN
ajpf-645	47	18	-	-	PUNCT
ajpf-645	47	19	r	r	VERB
ajpf-645	47	20	-	-	PUNCT
ajpf-645	47	21	his	his	PRON
ajpf-645	47	22	/	/	SYM
ajpf-645	47	23	ecori	ecori	NOUN
ajpf-645	47	24	gaattcatggtgatggtgatgatggccgccgttatgattaccgc	gaattcatggtgatggtgatgatggccgccgttatgattaccgc	NOUN
ajpf-645	47	25	sbla	sbla	NOUN
ajpf-645	47	26	-	-	PUNCT
ajpf-645	47	27	f	f	NOUN
ajpf-645	47	28	-	-	PUNCT
ajpf-645	47	29	bsphi	bsphi	NOUN
ajpf-645	47	30	tcatgagtattcaacatttccgtg	tcatgagtattcaacatttccgtg	NOUN
ajpf-645	47	31	sbla	sbla	NOUN
ajpf-645	47	32	-	-	PUNCT
ajpf-645	47	33	r	r	NOUN
ajpf-645	47	34	-	-	PUNCT
ajpf-645	47	35	his	his	PRON
ajpf-645	47	36	/	/	SYM
ajpf-645	47	37	ecori	ecori	NOUN
ajpf-645	47	38	gaattcatggtgatggtgatgatgttcagcatcttttactttca	gaattcatggtgatggtgatgatgttcagcatcttttactttca	ADJ
ajpf-645	47	39	sompw	sompw	NOUN
ajpf-645	47	40	-	-	PUNCT
ajpf-645	47	41	f	f	NOUN
ajpf-645	47	42	-	-	PUNCT
ajpf-645	47	43	bsphi	bsphi	VERB
ajpf-645	47	44	tcatgaaaaaatttacagtggcgg	tcatgaaaaaatttacagtggcgg	NOUN
ajpf-645	47	45	sompw	sompw	NOUN
ajpf-645	47	46	-	-	PUNCT
ajpf-645	47	47	r	r	NOUN
ajpf-645	47	48	-	-	PUNCT
ajpf-645	47	49	his	his	PRON
ajpf-645	47	50	/	/	SYM
ajpf-645	47	51	ecori	ecori	NOUN
ajpf-645	47	52	gaattcatggtgatggtgatgatgtccggcttcgtgcgcgaacg	gaattcatggtgatggtgatgatgtccggcttcgtgcgcgaacg	NOUN
ajpf-645	47	53	sstfa	sstfa	PROPN
ajpf-645	47	54	-	-	PUNCT
ajpf-645	47	55	f	f	NOUN
ajpf-645	47	56	-	-	PUNCT
ajpf-645	47	57	bsphi	bsphi	NOUN
ajpf-645	47	58	tcatgaatacagcagtaaaagctg	tcatgaatacagcagtaaaagctg	NOUN
ajpf-645	47	59	sstfa	sstfa	ADJ
ajpf-645	47	60	-	-	PUNCT
ajpf-645	47	61	r	r	NOUN
ajpf-645	47	62	-	-	PUNCT
ajpf-645	47	63	his	his	PRON
ajpf-645	47	64	/	/	SYM
ajpf-645	47	65	ecori	ecori	NOUN
ajpf-645	47	66	gaattcatggtgatggtgatgatgaccggtaaaagtcaccgtac	gaattcatggtgatggtgatgatgaccggtaaaagtcaccgtac	NOUN
ajpf-645	47	67	concentrations	concentration	NOUN
ajpf-645	47	68	for	for	ADP
ajpf-645	47	69	each	each	DET
ajpf-645	47	70	culture	culture	NOUN
ajpf-645	47	71	condition	condition	NOUN
ajpf-645	47	72	:	:	PUNCT
ajpf-645	48	1	ampicillin	ampicillin	PROPN
ajpf-645	48	2	,	,	PUNCT
ajpf-645	48	3	100	100	NUM
ajpf-645	48	4	g	g	NOUN
ajpf-645	48	5	/	/	SYM
ajpf-645	48	6	ml	ml	PROPN
ajpf-645	48	7	;	;	PUNCT
ajpf-645	48	8	streptomycin	streptomycin	PROPN
ajpf-645	48	9	,	,	PUNCT
ajpf-645	48	10	50	50	NUM
ajpf-645	48	11	g	g	NOUN
ajpf-645	48	12	/	/	SYM
ajpf-645	48	13	ml	ml	NOUN
ajpf-645	48	14	.	.	PUNCT
ajpf-645	49	1	dna	dna	PROPN
ajpf-645	49	2	manipulations	manipulation	NOUN
ajpf-645	49	3	general	general	ADJ
ajpf-645	49	4	dna	dna	PROPN
ajpf-645	49	5	manipulations	manipulation	NOUN
ajpf-645	49	6	were	be	AUX
ajpf-645	49	7	conducted	conduct	VERB
ajpf-645	49	8	as	as	SCONJ
ajpf-645	49	9	described	describe	VERB
ajpf-645	49	10	by	by	ADP
ajpf-645	49	11	sambrook	sambrook	PROPN
ajpf-645	49	12	et	et	PROPN
ajpf-645	49	13	al	al	PROPN
ajpf-645	49	14	.	.	PROPN
ajpf-645	50	1	(	(	PUNCT
ajpf-645	50	2	1989	1989	NUM
ajpf-645	50	3	)	)	PUNCT
ajpf-645	50	4	.	.	PUNCT
ajpf-645	51	1	plasmids	plasmid	NOUN
ajpf-645	51	2	were	be	AUX
ajpf-645	51	3	introduced	introduce	VERB
ajpf-645	51	4	into	into	ADP
ajpf-645	51	5	e.	e.	PROPN
ajpf-645	51	6	coli	coli	PROPN
ajpf-645	51	7	competent	competent	ADJ
ajpf-645	51	8	cells	cell	NOUN
ajpf-645	51	9	by	by	ADP
ajpf-645	51	10	heat	heat	NOUN
ajpf-645	51	11	-	-	PUNCT
ajpf-645	51	12	shock	shock	NOUN
ajpf-645	51	13	with	with	ADP
ajpf-645	51	14	rbcl2	rbcl2	PROPN
ajpf-645	51	15	treatment	treatment	NOUN
ajpf-645	51	16	(	(	PUNCT
ajpf-645	51	17	hanahan	hanahan	PROPN
ajpf-645	51	18	,	,	PUNCT
ajpf-645	51	19	1983	1983	NUM
ajpf-645	51	20	)	)	PUNCT
ajpf-645	51	21	and	and	CCONJ
ajpf-645	51	22	were	be	AUX
ajpf-645	51	23	introduced	introduce	VERB
ajpf-645	51	24	into	into	ADP
ajpf-645	51	25	salmonella	salmonella	NOUN
ajpf-645	51	26	competent	competent	ADJ
ajpf-645	51	27	cells	cell	NOUN
ajpf-645	51	28	by	by	ADP
ajpf-645	51	29	electroporation	electroporation	NOUN
ajpf-645	51	30	with	with	ADP
ajpf-645	51	31	10	10	NUM
ajpf-645	51	32	%	%	NOUN
ajpf-645	51	33	glycerol	glycerol	NOUN
ajpf-645	51	34	treatment	treatment	NOUN
ajpf-645	51	35	(	(	PUNCT
ajpf-645	51	36	sambrook	sambrook	NOUN
ajpf-645	51	37	et	et	PROPN
ajpf-645	51	38	al	al	PROPN
ajpf-645	51	39	.	.	PROPN
ajpf-645	51	40	,	,	PUNCT
ajpf-645	51	41	1989	1989	NUM
ajpf-645	51	42	)	)	PUNCT
ajpf-645	51	43	.	.	PUNCT
ajpf-645	52	1	nucleotide	nucleotide	NOUN
ajpf-645	52	2	sequencing	sequencing	NOUN
ajpf-645	52	3	was	be	AUX
ajpf-645	52	4	conducted	conduct	VERB
ajpf-645	52	5	by	by	ADP
ajpf-645	52	6	using	use	VERB
ajpf-645	52	7	an	an	DET
ajpf-645	52	8	abi	abi	NOUN
ajpf-645	52	9	373	373	NUM
ajpf-645	52	10	automatic	automatic	ADJ
ajpf-645	52	11	sequencer	sequencer	NOUN
ajpf-645	52	12	(	(	PUNCT
ajpf-645	52	13	pe	pe	ADP
ajpf-645	52	14	applied	apply	VERB
ajpf-645	52	15	biosystems	biosystem	NOUN
ajpf-645	52	16	)	)	PUNCT
ajpf-645	52	17	.	.	PUNCT
ajpf-645	53	1	cloning	cloning	NOUN
ajpf-645	53	2	of	of	ADP
ajpf-645	53	3	genes	gene	NOUN
ajpf-645	53	4	for	for	ADP
ajpf-645	53	5	signal	signal	ADJ
ajpf-645	53	6	sequence	sequence	NOUN
ajpf-645	53	7	the	the	DET
ajpf-645	53	8	dna	dna	PROPN
ajpf-645	53	9	regions	region	NOUN
ajpf-645	53	10	corresponding	correspond	VERB
ajpf-645	53	11	to	to	PART
ajpf-645	53	12	signal	signal	VERB
ajpf-645	53	13	peptide	peptide	NOUN
ajpf-645	53	14	of	of	ADP
ajpf-645	53	15	bla	bla	PROPN
ajpf-645	53	16	,	,	PUNCT
ajpf-645	53	17	agfa	agfa	PROPN
ajpf-645	53	18	,	,	PUNCT
ajpf-645	53	19	ompw	ompw	NOUN
ajpf-645	53	20	and	and	CCONJ
ajpf-645	53	21	stfa	stfa	NOUN
ajpf-645	53	22	was	be	AUX
ajpf-645	53	23	pcr	pcr	NOUN
ajpf-645	53	24	-	-	PUNCT
ajpf-645	53	25	amplified	amplify	VERB
ajpf-645	53	26	from	from	ADP
ajpf-645	53	27	pya3493	pya3493	NOUN
ajpf-645	53	28	or	or	CCONJ
ajpf-645	53	29	s.	s.	PROPN
ajpf-645	53	30	typhimurium	typhimurium	PROPN
ajpf-645	53	31	3339	3339	PROPN
ajpf-645	53	32	chromosome	chromosome	NOUN
ajpf-645	53	33	as	as	ADP
ajpf-645	53	34	a	a	DET
ajpf-645	53	35	template	template	NOUN
ajpf-645	53	36	.	.	PUNCT
ajpf-645	54	1	the	the	DET
ajpf-645	54	2	oligonucleotide	oligonucleotide	ADJ
ajpf-645	54	3	primers	primer	NOUN
ajpf-645	54	4	used	use	VERB
ajpf-645	54	5	in	in	ADP
ajpf-645	54	6	pcr	pcr	ADJ
ajpf-645	54	7	-	-	PUNCT
ajpf-645	54	8	amplification	amplification	NOUN
ajpf-645	54	9	were	be	AUX
ajpf-645	54	10	designed	design	VERB
ajpf-645	54	11	to	to	PART
ajpf-645	54	12	insert	insert	VERB
ajpf-645	54	13	restriction	restriction	NOUN
ajpf-645	54	14	sites	site	NOUN
ajpf-645	54	15	for	for	ADP
ajpf-645	54	16	bsphi	bsphi	NOUN
ajpf-645	54	17	and	and	CCONJ
ajpf-645	54	18	ecori	ecori	NOUN
ajpf-645	54	19	(	(	PUNCT
ajpf-645	54	20	table	table	NOUN
ajpf-645	54	21	2	2	NUM
ajpf-645	54	22	)	)	PUNCT
ajpf-645	54	23	.	.	PUNCT
ajpf-645	55	1	the	the	DET
ajpf-645	55	2	primers	primer	NOUN
ajpf-645	55	3	were	be	AUX
ajpf-645	55	4	also	also	ADV
ajpf-645	55	5	designed	design	VERB
ajpf-645	55	6	to	to	PART
ajpf-645	55	7	include	include	VERB
ajpf-645	55	8	his	his	PRON
ajpf-645	55	9	6	6	NUM
ajpf-645	55	10	-	-	PUNCT
ajpf-645	55	11	epitope	epitope	NOUN
ajpf-645	55	12	at	at	ADP
ajpf-645	55	13	the	the	DET
ajpf-645	55	14	c	c	NOUN
ajpf-645	55	15	-	-	PUNCT
ajpf-645	55	16	terminal	terminal	NOUN
ajpf-645	55	17	of	of	ADP
ajpf-645	55	18	each	each	DET
ajpf-645	55	19	signal	signal	NOUN
ajpf-645	55	20	peptide	peptide	NOUN
ajpf-645	55	21	,	,	PUNCT
ajpf-645	55	22	which	which	PRON
ajpf-645	55	23	allows	allow	VERB
ajpf-645	55	24	the	the	DET
ajpf-645	55	25	detection	detection	NOUN
ajpf-645	55	26	of	of	ADP
ajpf-645	55	27	expressed	express	VERB
ajpf-645	55	28	signal	signal	NOUN
ajpf-645	55	29	peptides	peptide	NOUN
ajpf-645	55	30	by	by	ADP
ajpf-645	55	31	immunoblot	immunoblot	NOUN
ajpf-645	55	32	with	with	ADP
ajpf-645	55	33	anti	anti	ADJ
ajpf-645	55	34	-	-	ADJ
ajpf-645	55	35	his6	his6	ADJ
ajpf-645	55	36	-	-	PUNCT
ajpf-645	55	37	epitope	epitope	NOUN
ajpf-645	55	38	antibody	antibody	NOUN
ajpf-645	55	39	.	.	PUNCT
ajpf-645	56	1	pcr	pcr	ADJ
ajpf-645	56	2	reaction	reaction	NOUN
ajpf-645	56	3	conditions	condition	NOUN
ajpf-645	56	4	were	be	AUX
ajpf-645	56	5	as	as	SCONJ
ajpf-645	56	6	follows	follow	VERB
ajpf-645	56	7	:	:	PUNCT
ajpf-645	56	8	denaturation	denaturation	NOUN
ajpf-645	56	9	at	at	ADP
ajpf-645	56	10	95	95	NUM
ajpf-645	56	11	°	°	ADP
ajpf-645	56	12	c	c	NOUN
ajpf-645	56	13	for	for	ADP
ajpf-645	56	14	30	30	NUM
ajpf-645	56	15	s	s	NOUN
ajpf-645	56	16	,	,	PUNCT
ajpf-645	56	17	primer	primer	NOUN
ajpf-645	56	18	annealing	annealing	NOUN
ajpf-645	56	19	at	at	ADP
ajpf-645	56	20	the	the	DET
ajpf-645	56	21	temperature	temperature	NOUN
ajpf-645	56	22	of	of	ADP
ajpf-645	56	23	primer	primer	NOUN
ajpf-645	56	24	’s	’s	PART
ajpf-645	56	25	melting	melt	VERB
ajpf-645	56	26	temperature	temperature	NOUN
ajpf-645	56	27	(	(	PUNCT
ajpf-645	56	28	tm	tm	NOUN
ajpf-645	56	29	)	)	PUNCT
ajpf-645	56	30	for	for	ADP
ajpf-645	56	31	30	30	NUM
ajpf-645	56	32	s	s	NOUN
ajpf-645	56	33	,	,	PUNCT
ajpf-645	56	34	polymerization	polymerization	NOUN
ajpf-645	56	35	at	at	ADP
ajpf-645	56	36	72	72	NUM
ajpf-645	56	37	°	°	ADP
ajpf-645	56	38	c	c	NOUN
ajpf-645	56	39	for	for	ADP
ajpf-645	56	40	30	30	NUM
ajpf-645	56	41	s	s	NOUN
ajpf-645	56	42	~	~	SYM
ajpf-645	56	43	3	3	NUM
ajpf-645	56	44	min	min	NOUN
ajpf-645	56	45	depending	depend	VERB
ajpf-645	56	46	on	on	ADP
ajpf-645	56	47	the	the	DET
ajpf-645	56	48	length	length	NOUN
ajpf-645	56	49	of	of	ADP
ajpf-645	56	50	dna	dna	PROPN
ajpf-645	56	51	fragment	fragment	NOUN
ajpf-645	56	52	and	and	CCONJ
ajpf-645	56	53	a	a	DET
ajpf-645	56	54	final	final	ADJ
ajpf-645	56	55	extension	extension	NOUN
ajpf-645	56	56	at	at	ADP
ajpf-645	56	57	72	72	NUM
ajpf-645	56	58	°	°	ADP
ajpf-645	56	59	c	c	NOUN
ajpf-645	56	60	for	for	ADP
ajpf-645	56	61	10	10	NUM
ajpf-645	56	62	min	min	NOUN
ajpf-645	56	63	.	.	PUNCT
ajpf-645	57	1	the	the	DET
ajpf-645	57	2	amplified	amplify	VERB
ajpf-645	57	3	dna	dna	NOUN
ajpf-645	57	4	fragments	fragment	NOUN
ajpf-645	57	5	were	be	AUX
ajpf-645	57	6	cloned	clone	VERB
ajpf-645	57	7	into	into	ADP
ajpf-645	57	8	t	t	PROPN
ajpf-645	57	9	-	-	PUNCT
ajpf-645	57	10	vector	vector	NOUN
ajpf-645	57	11	(	(	PUNCT
ajpf-645	57	12	promega	promega	PROPN
ajpf-645	57	13	)	)	PUNCT
ajpf-645	57	14	as	as	ADP
ajpf-645	57	15	a	a	DET
ajpf-645	57	16	temporary	temporary	ADJ
ajpf-645	57	17	step	step	NOUN
ajpf-645	57	18	.	.	PUNCT
ajpf-645	58	1	the	the	DET
ajpf-645	58	2	bsphi	bsphi	PROPN
ajpf-645	58	3	-ecori	-ecori	PROPN
ajpf-645	58	4	dna	dna	PROPN
ajpf-645	58	5	segment	segment	NOUN
ajpf-645	58	6	isolated	isolate	VERB
ajpf-645	58	7	from	from	ADP
ajpf-645	58	8	the	the	DET
ajpf-645	58	9	recombinant	recombinant	ADJ
ajpf-645	58	10	t	t	PROPN
ajpf-645	58	11	-	-	PUNCT
ajpf-645	58	12	vector	vector	NOUN
ajpf-645	58	13	was	be	AUX
ajpf-645	58	14	cloned	clone	VERB
ajpf-645	58	15	into	into	ADP
ajpf-645	58	16	a	a	DET
ajpf-645	58	17	plasmid	plasmid	NOUN
ajpf-645	58	18	pya3342	pya3342	NOUN
ajpf-645	58	19	digested	digest	VERB
ajpf-645	58	20	with	with	ADP
ajpf-645	58	21	ncoi	ncoi	NOUN
ajpf-645	58	22	and	and	CCONJ
ajpf-645	58	23	ecori	ecori	PROPN
ajpf-645	58	24	.	.	PUNCT
ajpf-645	59	1	sds	sds	NOUN
ajpf-645	59	2	-	-	PUNCT
ajpf-645	59	3	page	page	NOUN
ajpf-645	59	4	(	(	PUNCT
ajpf-645	59	5	polyacrylamide	polyacrylamide	NOUN
ajpf-645	59	6	gel	gel	NOUN
ajpf-645	59	7	electrophoresis	electrophoresis	NOUN
ajpf-645	59	8	)	)	PUNCT
ajpf-645	59	9	and	and	CCONJ
ajpf-645	59	10	immunoblot	immunoblot	VERB
ajpf-645	59	11	bacterial	bacterial	ADJ
ajpf-645	59	12	culture	culture	NOUN
ajpf-645	59	13	broth	broth	NOUN
ajpf-645	59	14	was	be	AUX
ajpf-645	59	15	centrifuged	centrifuge	VERB
ajpf-645	59	16	for	for	ADP
ajpf-645	59	17	10	10	NUM
ajpf-645	59	18	min	min	NOUN
ajpf-645	59	19	at	at	ADP
ajpf-645	59	20	5,000	5,000	NUM
ajpf-645	59	21	x	x	SYM
ajpf-645	59	22	g	g	NOUN
ajpf-645	59	23	to	to	PART
ajpf-645	59	24	separate	separate	VERB
ajpf-645	59	25	the	the	DET
ajpf-645	59	26	cells	cell	NOUN
ajpf-645	59	27	from	from	ADP
ajpf-645	59	28	the	the	DET
ajpf-645	59	29	culture	culture	NOUN
ajpf-645	59	30	supernatant	supernatant	NOUN
ajpf-645	59	31	.	.	PUNCT
ajpf-645	60	1	the	the	DET
ajpf-645	60	2	supernatants	supernatant	NOUN
ajpf-645	60	3	was	be	AUX
ajpf-645	60	4	collected	collect	VERB
ajpf-645	60	5	and	and	CCONJ
ajpf-645	60	6	further	far	ADV
ajpf-645	60	7	used	use	VERB
ajpf-645	60	8	for	for	ADP
ajpf-645	60	9	the	the	DET
ajpf-645	60	10	analysis	analysis	NOUN
ajpf-645	60	11	of	of	ADP
ajpf-645	60	12	secreted	secrete	VERB
ajpf-645	60	13	proteins	protein	NOUN
ajpf-645	60	14	.	.	PUNCT
ajpf-645	61	1	the	the	DET
ajpf-645	61	2	precipitated	precipitate	VERB
ajpf-645	61	3	cell	cell	NOUN
ajpf-645	61	4	pellet	pellet	NOUN
ajpf-645	61	5	was	be	AUX
ajpf-645	61	6	washed	wash	VERB
ajpf-645	61	7	twice	twice	ADV
ajpf-645	61	8	with	with	ADP
ajpf-645	61	9	0.85	0.85	NUM
ajpf-645	61	10	%	%	NOUN
ajpf-645	61	11	nacl	nacl	NOUN
ajpf-645	61	12	and	and	CCONJ
ajpf-645	61	13	then	then	ADV
ajpf-645	61	14	resuspended	resuspend	VERB
ajpf-645	61	15	in	in	ADP
ajpf-645	61	16	10	10	NUM
ajpf-645	61	17	mm	mm	PROPN
ajpf-645	61	18	tris	tris	NOUN
ajpf-645	61	19	hcl	hcl	PROPN
ajpf-645	61	20	,	,	PUNCT
ajpf-645	61	21	ph	ph	VERB
ajpf-645	61	22	7.5	7.5	NUM
ajpf-645	61	23	.	.	PUNCT
ajpf-645	62	1	the	the	DET
ajpf-645	62	2	suspended	suspend	VERB
ajpf-645	62	3	cells	cell	NOUN
ajpf-645	62	4	were	be	AUX
ajpf-645	62	5	mixed	mix	VERB
ajpf-645	62	6	with	with	ADP
ajpf-645	62	7	sds	sds	PROPN
ajpf-645	62	8	gel	gel	NOUN
ajpf-645	62	9	loading	loading	NOUN
ajpf-645	62	10	buffer	buffer	NOUN
ajpf-645	62	11	and	and	CCONJ
ajpf-645	62	12	lysed	lyse	VERB
ajpf-645	62	13	thermally	thermally	ADV
ajpf-645	62	14	.	.	PUNCT
ajpf-645	63	1	the	the	DET
ajpf-645	63	2	65	65	NUM
ajpf-645	63	3	g	g	PROPN
ajpf-645	63	4	proteins	protein	NOUN
ajpf-645	63	5	of	of	ADP
ajpf-645	63	6	total	total	ADJ
ajpf-645	63	7	cell	cell	NOUN
ajpf-645	63	8	lysates	lysate	NOUN
ajpf-645	63	9	were	be	AUX
ajpf-645	63	10	then	then	ADV
ajpf-645	63	11	separated	separate	VERB
ajpf-645	63	12	through	through	ADP
ajpf-645	63	13	sdspage	sdspage	NOUN
ajpf-645	63	14	and	and	CCONJ
ajpf-645	63	15	the	the	DET
ajpf-645	63	16	separated	separate	VERB
ajpf-645	63	17	bands	band	NOUN
ajpf-645	63	18	were	be	AUX
ajpf-645	63	19	visualized	visualize	VERB
ajpf-645	63	20	by	by	ADP
ajpf-645	63	21	coomassie	coomassie	ADJ
ajpf-645	63	22	staining	stain	VERB
ajpf-645	63	23	(	(	PUNCT
ajpf-645	63	24	sambrook	sambrook	PROPN
ajpf-645	63	25	et	et	PROPN
ajpf-645	63	26	al	al	PROPN
ajpf-645	63	27	.	.	PROPN
ajpf-645	63	28	,	,	PUNCT
ajpf-645	63	29	1989	1989	NUM
ajpf-645	63	30	)	)	PUNCT
ajpf-645	63	31	.	.	PUNCT
ajpf-645	64	1	for	for	ADP
ajpf-645	64	2	the	the	DET
ajpf-645	64	3	analysis	analysis	NOUN
ajpf-645	64	4	of	of	ADP
ajpf-645	64	5	secreted	secreted	ADJ
ajpf-645	64	6	proteins	protein	NOUN
ajpf-645	64	7	,	,	PUNCT
ajpf-645	64	8	the	the	DET
ajpf-645	64	9	cultural	cultural	ADJ
ajpf-645	64	10	supernatant	supernatant	NOUN
ajpf-645	64	11	was	be	AUX
ajpf-645	64	12	concentrated	concentrate	VERB
ajpf-645	64	13	for	for	ADP
ajpf-645	64	14	1	1	NUM
ajpf-645	64	15	h	h	NOUN
ajpf-645	64	16	in	in	ADP
ajpf-645	64	17	icecold	icecold	PROPN
ajpf-645	64	18	tca	tca	PROPN
ajpf-645	64	19	(	(	PUNCT
ajpf-645	64	20	trichloroacetic	trichloroacetic	ADJ
ajpf-645	64	21	acid	acid	NOUN
ajpf-645	64	22	)	)	PUNCT
ajpf-645	64	23	solution	solution	NOUN
ajpf-645	64	24	(	(	PUNCT
ajpf-645	64	25	hoong	hoong	PROPN
ajpf-645	64	26	et	et	PROPN
ajpf-645	64	27	al	al	PROPN
ajpf-645	64	28	.	.	PROPN
ajpf-645	64	29	,	,	PUNCT
ajpf-645	64	30	1995	1995	NUM
ajpf-645	64	31	;	;	PUNCT
ajpf-645	64	32	ma	ma	PROPN
ajpf-645	64	33	et	et	PROPN
ajpf-645	64	34	al	al	PROPN
ajpf-645	64	35	.	.	PROPN
ajpf-645	64	36	,	,	PUNCT
ajpf-645	64	37	1996	1996	NUM
ajpf-645	64	38	)	)	PUNCT
ajpf-645	64	39	and	and	CCONJ
ajpf-645	64	40	then	then	ADV
ajpf-645	64	41	8	8	NUM
ajpf-645	64	42	µg	µg	ADV
ajpf-645	64	43	secreted	secrete	VERB
ajpf-645	64	44	proteins	protein	NOUN
ajpf-645	64	45	were	be	AUX
ajpf-645	64	46	separated	separate	VERB
ajpf-645	64	47	by	by	ADP
ajpf-645	64	48	sds	sds	PROPN
ajpf-645	64	49	page	page	NOUN
ajpf-645	64	50	.	.	PUNCT
ajpf-645	65	1	for	for	ADP
ajpf-645	65	2	immunoblotting	immunoblotte	VERB
ajpf-645	65	3	,	,	PUNCT
ajpf-645	65	4	proteins	protein	NOUN
ajpf-645	65	5	separated	separate	VERB
ajpf-645	65	6	by	by	ADP
ajpf-645	65	7	sds	sds	PROPN
ajpf-645	65	8	-	-	PUNCT
ajpf-645	65	9	page	page	NOUN
ajpf-645	65	10	were	be	AUX
ajpf-645	65	11	electrophoretically	electrophoretically	ADV
ajpf-645	65	12	transferred	transfer	VERB
ajpf-645	65	13	onto	onto	ADP
ajpf-645	65	14	the	the	DET
ajpf-645	65	15	nitrocellulose	nitrocellulose	NOUN
ajpf-645	65	16	membranes	membrane	NOUN
ajpf-645	65	17	.	.	PUNCT
ajpf-645	66	1	the	the	DET
ajpf-645	66	2	immunoblotting	immunoblotting	NOUN
ajpf-645	66	3	was	be	AUX
ajpf-645	66	4	conducted	conduct	VERB
ajpf-645	66	5	in	in	ADP
ajpf-645	66	6	accordance	accordance	NOUN
ajpf-645	66	7	with	with	ADP
ajpf-645	66	8	the	the	DET
ajpf-645	66	9	protocols	protocol	NOUN
ajpf-645	66	10	established	establish	VERB
ajpf-645	66	11	by	by	ADP
ajpf-645	66	12	sambrook	sambrook	PROPN
ajpf-645	66	13	et	et	PROPN
ajpf-645	66	14	al	al	PROPN
ajpf-645	66	15	.	.	PROPN
ajpf-645	67	1	(	(	PUNCT
ajpf-645	67	2	1989	1989	NUM
ajpf-645	67	3	)	)	PUNCT
ajpf-645	67	4	.	.	PUNCT
ajpf-645	68	1	the	the	DET
ajpf-645	68	2	pspa	pspa	ADJ
ajpf-645	68	3	protein	protein	NOUN
ajpf-645	68	4	and	and	CCONJ
ajpf-645	68	5	his6	his6	NOUN
ajpf-645	68	6	-	-	PUNCT
ajpf-645	68	7	tagged	tag	VERB
ajpf-645	68	8	signal	signal	NOUN
ajpf-645	68	9	peptides	peptide	NOUN
ajpf-645	68	10	were	be	AUX
ajpf-645	68	11	specifically	specifically	ADV
ajpf-645	68	12	recognized	recognize	VERB
ajpf-645	68	13	by	by	ADP
ajpf-645	68	14	the	the	DET
ajpf-645	68	15	use	use	NOUN
ajpf-645	68	16	of	of	ADP
ajpf-645	68	17	xi126	xi126	PROPN
ajpf-645	68	18	monoclonal	monoclonal	NOUN
ajpf-645	68	19	antibody	antibody	NOUN
ajpf-645	68	20	(	(	PUNCT
ajpf-645	68	21	mcdaniel	mcdaniel	PROPN
ajpf-645	68	22	et	et	PROPN
ajpf-645	68	23	al	al	PROPN
ajpf-645	68	24	.	.	PROPN
ajpf-645	68	25	,	,	PUNCT
ajpf-645	68	26	1984	1984	NUM
ajpf-645	68	27	)	)	PUNCT
ajpf-645	68	28	and	and	CCONJ
ajpf-645	68	29	his6tag	his6tag	NOUN
ajpf-645	68	30	monoclonal	monoclonal	NOUN
ajpf-645	68	31	antibody	antibody	NOUN
ajpf-645	68	32	(	(	PUNCT
ajpf-645	68	33	southern	southern	ADJ
ajpf-645	68	34	biotech	biotech	NOUN
ajpf-645	68	35	)	)	PUNCT
ajpf-645	68	36	,	,	PUNCT
ajpf-645	68	37	respectively	respectively	ADV
ajpf-645	68	38	.	.	PUNCT
ajpf-645	69	1	goat	goat	PROPN
ajpf-645	69	2	anti	anti	PROPN
ajpf-645	69	3	-	-	ADJ
ajpf-645	69	4	mouse	mouse	ADJ
ajpf-645	69	5	igg	igg	PROPN
ajpf-645	69	6	,	,	PUNCT
ajpf-645	69	7	horseradish	horseradish	ADJ
ajpf-645	69	8	peroxidase	peroxidase	NOUN
ajpf-645	69	9	(	(	PUNCT
ajpf-645	69	10	hrp	hrp	PROPN
ajpf-645	69	11	)	)	PUNCT
ajpf-645	69	12	conjugated	conjugate	VERB
ajpf-645	69	13	antibody	antibody	NOUN
ajpf-645	69	14	was	be	AUX
ajpf-645	69	15	used	use	VERB
ajpf-645	69	16	as	as	ADP
ajpf-645	69	17	secondary	secondary	ADJ
ajpf-645	69	18	antibody	antibody	NOUN
ajpf-645	69	19	and	and	CCONJ
ajpf-645	69	20	the	the	DET
ajpf-645	69	21	4	4	NUM
ajpf-645	69	22	-	-	SYM
ajpf-645	69	23	choloro-1naphthol	choloro-1naphthol	NOUN
ajpf-645	69	24	was	be	AUX
ajpf-645	69	25	used	use	VERB
ajpf-645	69	26	for	for	ADP
ajpf-645	69	27	the	the	DET
ajpf-645	69	28	substrate	substrate	NOUN
ajpf-645	69	29	of	of	ADP
ajpf-645	69	30	hrp	hrp	PROPN
ajpf-645	69	31	.	.	PUNCT
ajpf-645	70	1	results	result	NOUN
ajpf-645	70	2	and	and	CCONJ
ajpf-645	70	3	discussion	discussion	NOUN
ajpf-645	70	4	background	background	NOUN
ajpf-645	70	5	of	of	ADP
ajpf-645	70	6	construction	construction	NOUN
ajpf-645	70	7	for	for	ADP
ajpf-645	70	8	new	new	ADJ
ajpf-645	70	9	foreign	foreign	ADJ
ajpf-645	70	10	antigencarrying	antigencarrye	VERB
ajpf-645	70	11	systems	system	NOUN
ajpf-645	70	12	and	and	CCONJ
ajpf-645	70	13	the	the	DET
ajpf-645	70	14	results	result	NOUN
ajpf-645	70	15	most	most	ADJ
ajpf-645	70	16	of	of	ADP
ajpf-645	70	17	the	the	DET
ajpf-645	70	18	bacteria	bacteria	NOUN
ajpf-645	70	19	operate	operate	VERB
ajpf-645	70	20	general	general	ADJ
ajpf-645	70	21	secretory	secretory	ADJ
ajpf-645	70	22	pathway	pathway	NOUN
ajpf-645	70	23	(	(	PUNCT
ajpf-645	70	24	gsp	gsp	PROPN
ajpf-645	70	25	)	)	PUNCT
ajpf-645	70	26	to	to	PART
ajpf-645	70	27	translocate	translocate	VERB
ajpf-645	70	28	proteins	protein	NOUN
ajpf-645	70	29	from	from	ADP
ajpf-645	70	30	cytoplasm	cytoplasm	NOUN
ajpf-645	70	31	to	to	ADP
ajpf-645	70	32	periplasmic	periplasmic	ADJ
ajpf-645	70	33	space	space	NOUN
ajpf-645	70	34	,	,	PUNCT
ajpf-645	70	35	outer	outer	ADJ
ajpf-645	70	36	membrane	membrane	NOUN
ajpf-645	70	37	or	or	CCONJ
ajpf-645	70	38	extracellular	extracellular	ADJ
ajpf-645	70	39	matrix	matrix	NOUN
ajpf-645	70	40	.	.	PUNCT
ajpf-645	71	1	the	the	DET
ajpf-645	71	2	ss	ss	NOUN
ajpf-645	71	3	located	locate	VERB
ajpf-645	71	4	at	at	ADP
ajpf-645	71	5	nterminus	nterminus	NOUN
ajpf-645	71	6	of	of	ADP
ajpf-645	71	7	the	the	DET
ajpf-645	71	8	secreting	secrete	VERB
ajpf-645	71	9	protein	protein	NOUN
ajpf-645	71	10	leads	lead	VERB
ajpf-645	71	11	the	the	DET
ajpf-645	71	12	translocation	translocation	NOUN
ajpf-645	71	13	of	of	ADP
ajpf-645	71	14	the	the	DET
ajpf-645	71	15	protein	protein	NOUN
ajpf-645	71	16	to	to	PART
ajpf-645	71	17	membrane	membrane	VERB
ajpf-645	71	18	.	.	PUNCT
ajpf-645	72	1	in	in	ADP
ajpf-645	72	2	a	a	DET
ajpf-645	72	3	previous	previous	ADJ
ajpf-645	72	4	study	study	NOUN
ajpf-645	72	5	(	(	PUNCT
ajpf-645	72	6	kang	kang	PROPN
ajpf-645	72	7	et	et	PROPN
ajpf-645	72	8	al	al	PROPN
ajpf-645	72	9	.	.	PROPN
ajpf-645	72	10	,	,	PUNCT
ajpf-645	72	11	2002	2002	NUM
ajpf-645	72	12	)	)	PUNCT
ajpf-645	72	13	a	a	DET
ajpf-645	72	14	plasmid	plasmid	ADJ
ajpf-645	72	15	system	system	NOUN
ajpf-645	72	16	(	(	PUNCT
ajpf-645	72	17	pya3493	pya3493	ADJ
ajpf-645	72	18	)	)	PUNCT
ajpf-645	72	19	was	be	AUX
ajpf-645	72	20	generated	generate	VERB
ajpf-645	72	21	for	for	ADP
ajpf-645	72	22	the	the	DET
ajpf-645	72	23	secretion	secretion	NOUN
ajpf-645	72	24	of	of	ADP
ajpf-645	72	25	proteins	protein	NOUN
ajpf-645	72	26	through	through	ADP
ajpf-645	72	27	gsp	gsp	PROPN
ajpf-645	72	28	.	.	PUNCT
ajpf-645	73	1	main	main	ADJ
ajpf-645	73	2	idea	idea	NOUN
ajpf-645	73	3	of	of	ADP
ajpf-645	73	4	the	the	DET
ajpf-645	73	5	pya3493	pya3493	ADJ
ajpf-645	73	6	based	base	VERB
ajpf-645	73	7	expression	expression	NOUN
ajpf-645	73	8	system	system	NOUN
ajpf-645	73	9	is	be	AUX
ajpf-645	73	10	the	the	DET
ajpf-645	73	11	fusion	fusion	NOUN
ajpf-645	73	12	of	of	ADP
ajpf-645	73	13	lactamase	lactamase	ADJ
ajpf-645	73	14	ss	ss	PROPN
ajpf-645	73	15	at	at	ADP
ajpf-645	73	16	the	the	DET
ajpf-645	73	17	n	n	CCONJ
ajpf-645	73	18	-	-	PUNCT
ajpf-645	73	19	terminus	terminus	NOUN
ajpf-645	73	20	of	of	ADP
ajpf-645	73	21	expressed	express	VERB
ajpf-645	73	22	proteins	protein	NOUN
ajpf-645	73	23	.	.	PUNCT
ajpf-645	74	1	in	in	ADP
ajpf-645	74	2	this	this	DET
ajpf-645	74	3	study	study	NOUN
ajpf-645	74	4	,	,	PUNCT
ajpf-645	74	5	we	we	PRON
ajpf-645	74	6	examined	examine	VERB
ajpf-645	74	7	protein	protein	NOUN
ajpf-645	74	8	secretion	secretion	NOUN
ajpf-645	74	9	capacity	capacity	NOUN
ajpf-645	74	10	by	by	ADP
ajpf-645	74	11	the	the	DET
ajpf-645	74	12	replacement	replacement	NOUN
ajpf-645	74	13	of	of	ADP
ajpf-645	74	14	-lactamase	-lactamase	PROPN
ajpf-645	74	15	ss	ss	PROPN
ajpf-645	74	16	with	with	ADP
ajpf-645	74	17	the	the	DET
ajpf-645	74	18	ss	ss	NOUN
ajpf-645	74	19	of	of	ADP
ajpf-645	74	20	other	other	ADJ
ajpf-645	74	21	proteins	protein	NOUN
ajpf-645	74	22	in	in	ADP
ajpf-645	74	23	pya3493	pya3493	ADJ
ajpf-645	74	24	.	.	PUNCT
ajpf-645	75	1	the	the	DET
ajpf-645	75	2	plasmid	plasmid	ADJ
ajpf-645	75	3	pya3493	pya3493	NOUN
ajpf-645	75	4	carries	carry	VERB
ajpf-645	75	5	an	an	DET
ajpf-645	75	6	aspartate	aspartate	NOUN
ajpf-645	75	7	semialdehyde	semialdehyde	ADJ
ajpf-645	75	8	dehydrogenase	dehydrogenase	NOUN
ajpf-645	75	9	(	(	PUNCT
ajpf-645	75	10	asd	asd	NOUN
ajpf-645	75	11	)	)	PUNCT
ajpf-645	75	12	gene	gene	NOUN
ajpf-645	75	13	,	,	PUNCT
ajpf-645	75	14	which	which	PRON
ajpf-645	75	15	is	be	AUX
ajpf-645	75	16	required	require	VERB
ajpf-645	75	17	for	for	ADP
ajpf-645	75	18	the	the	DET
ajpf-645	75	19	biosynthesis	biosynthesis	NOUN
ajpf-645	75	20	of	of	ADP
ajpf-645	75	21	mdiaminopimellic	mdiaminopimellic	ADJ
ajpf-645	75	22	acid	acid	NOUN
ajpf-645	75	23	(	(	PUNCT
ajpf-645	75	24	m	m	PROPN
ajpf-645	75	25	-	-	PUNCT
ajpf-645	75	26	dap	dap	NOUN
ajpf-645	75	27	)	)	PUNCT
ajpf-645	75	28	,	,	PUNCT
ajpf-645	75	29	a	a	DET
ajpf-645	75	30	component	component	NOUN
ajpf-645	75	31	of	of	ADP
ajpf-645	75	32	bacterial	bacterial	ADJ
ajpf-645	75	33	cell	cell	NOUN
ajpf-645	75	34	wall	wall	NOUN
ajpf-645	75	35	.	.	PUNCT
ajpf-645	76	1	the	the	DET
ajpf-645	76	2	“	"	PUNCT
ajpf-645	76	3	asd	asd	NOUN
ajpf-645	76	4	”	"	PUNCT
ajpf-645	76	5	gene	gene	NOUN
ajpf-645	76	6	in	in	ADP
ajpf-645	76	7	pya3493	pya3493	ADJ
ajpf-645	76	8	complements	complement	NOUN
ajpf-645	76	9	“	"	PUNCT
ajpf-645	76	10	asd	asd	ADJ
ajpf-645	76	11	”	"	PUNCT
ajpf-645	76	12	deficiency	deficiency	NOUN
ajpf-645	76	13	of	of	ADP
ajpf-645	76	14	bacterial	bacterial	ADJ
ajpf-645	76	15	hosts	host	NOUN
ajpf-645	76	16	,	,	PUNCT
ajpf-645	76	17	e.	e.	PROPN
ajpf-645	76	18	coli	coli	PROPN
ajpf-645	76	19	6212	6212	NOUN
ajpf-645	76	20	and	and	CCONJ
ajpf-645	76	21	s.	s.	PROPN
ajpf-645	76	22	typhimurium	typhimurium	PROPN
ajpf-645	76	23	8554	8554	PROPN
ajpf-645	76	24	(	(	PUNCT
ajpf-645	76	25	table	table	NOUN
ajpf-645	76	26	1	1	NUM
ajpf-645	76	27	)	)	PUNCT
ajpf-645	76	28	.	.	PUNCT
ajpf-645	77	1	multicloning	multiclone	VERB
ajpf-645	77	2	sites	site	NOUN
ajpf-645	77	3	(	(	PUNCT
ajpf-645	77	4	mcs	mcs	NOUN
ajpf-645	77	5	)	)	PUNCT
ajpf-645	77	6	is	be	AUX
ajpf-645	77	7	positioned	position	VERB
ajpf-645	77	8	at	at	ADP
ajpf-645	77	9	the	the	DET
ajpf-645	77	10	downstream	downstream	ADJ
ajpf-645	77	11	sequence	sequence	NOUN
ajpf-645	77	12	of	of	ADP
ajpf-645	77	13	ss	ss	PRON
ajpf-645	77	14	sequence	sequence	NOUN
ajpf-645	77	15	to	to	PART
ajpf-645	77	16	fuse	fuse	NOUN
ajpf-645	77	17	signal	signal	NOUN
ajpf-645	77	18	peptide	peptide	NOUN
ajpf-645	77	19	with	with	ADP
ajpf-645	77	20	the	the	DET
ajpf-645	77	21	protein	protein	NOUN
ajpf-645	77	22	of	of	ADP
ajpf-645	77	23	cloned	cloned	ADJ
ajpf-645	77	24	gene	gene	NOUN
ajpf-645	77	25	.	.	PUNCT
ajpf-645	78	1	to	to	PART
ajpf-645	78	2	examine	examine	VERB
ajpf-645	78	3	the	the	DET
ajpf-645	78	4	effect	effect	NOUN
ajpf-645	78	5	of	of	ADP
ajpf-645	78	6	the	the	DET
ajpf-645	78	7	type	type	NOUN
ajpf-645	78	8	of	of	ADP
ajpf-645	78	9	signal	signal	NOUN
ajpf-645	78	10	peptide	peptide	NOUN
ajpf-645	78	11	for	for	ADP
ajpf-645	78	12	protein	protein	NOUN
ajpf-645	78	13	secretion	secretion	NOUN
ajpf-645	78	14	,	,	PUNCT
ajpf-645	78	15	various	various	ADJ
ajpf-645	78	16	dna	dna	PROPN
ajpf-645	78	17	fragments	fragment	NOUN
ajpf-645	78	18	corresponding	correspond	VERB
ajpf-645	78	19	to	to	ADP
ajpf-645	78	20	the	the	DET
ajpf-645	78	21	signal	signal	ADJ
ajpf-645	78	22	peptide	peptide	NOUN
ajpf-645	78	23	of	of	ADP
ajpf-645	78	24	-lactamase	-lactamase	PROPN
ajpf-645	78	25	(	(	PUNCT
ajpf-645	78	26	bla	bla	NOUN
ajpf-645	78	27	)	)	PUNCT
ajpf-645	78	28	,	,	PUNCT
ajpf-645	78	29	agfa	agfa	PROPN
ajpf-645	78	30	,	,	PUNCT
ajpf-645	78	31	ompw	ompw	NOUN
ajpf-645	78	32	and	and	CCONJ
ajpf-645	78	33	stfa	stfa	NOUN
ajpf-645	78	34	were	be	AUX
ajpf-645	78	35	localized	localize	VERB
ajpf-645	78	36	at	at	ADP
ajpf-645	78	37	the	the	DET
ajpf-645	78	38	upstream	upstream	NOUN
ajpf-645	78	39	of	of	ADP
ajpf-645	78	40	mcs	mcs	NOUN
ajpf-645	78	41	:	:	PUNCT
ajpf-645	78	42	“	"	PUNCT
ajpf-645	78	43	bla	bla	PROPN
ajpf-645	78	44	ss	ss	NOUN
ajpf-645	78	45	”	"	PUNCT
ajpf-645	78	46	in	in	ADP
ajpf-645	78	47	pmmp62	pmmp62	NOUN
ajpf-645	78	48	,	,	PUNCT
ajpf-645	78	49	“	"	PUNCT
ajpf-645	78	50	agfa	agfa	PROPN
ajpf-645	78	51	ss	ss	PROPN
ajpf-645	78	52	”	"	PUNCT
ajpf-645	78	53	in	in	ADP
ajpf-645	78	54	pmmp63	pmmp63	ADJ
ajpf-645	78	55	,	,	PUNCT
ajpf-645	78	56	“	"	PUNCT
ajpf-645	78	57	ompw	ompw	NOUN
ajpf-645	78	58	ss	ss	NOUN
ajpf-645	78	59	”	"	PUNCT
ajpf-645	78	60	in	in	ADP
ajpf-645	78	61	pmmp64	pmmp64	NOUN
ajpf-645	78	62	and	and	CCONJ
ajpf-645	78	63	“	"	PUNCT
ajpf-645	78	64	stfa	stfa	VERB
ajpf-645	78	65	ss	ss	NOUN
ajpf-645	78	66	”	"	PUNCT
ajpf-645	78	67	in	in	ADP
ajpf-645	78	68	pmmp65	pmmp65	NOUN
ajpf-645	78	69	.	.	PUNCT
ajpf-645	79	1	the	the	DET
ajpf-645	79	2	c	c	NOUN
ajpf-645	79	3	-	-	PUNCT
ajpf-645	79	4	terminus	terminus	NOUN
ajpf-645	79	5	of	of	ADP
ajpf-645	79	6	each	each	DET
ajpf-645	79	7	signal	signal	ADJ
ajpf-645	79	8	peptides	peptide	NOUN
ajpf-645	79	9	figure	figure	NOUN
ajpf-645	79	10	1	1	NUM
ajpf-645	79	11	.	.	PUNCT
ajpf-645	80	1	schematic	schematic	ADJ
ajpf-645	80	2	diagram	diagram	NOUN
ajpf-645	80	3	of	of	ADP
ajpf-645	80	4	strategy	strategy	NOUN
ajpf-645	80	5	for	for	ADP
ajpf-645	80	6	cloning	cloning	NOUN
ajpf-645	80	7	of	of	ADP
ajpf-645	80	8	signal	signal	ADJ
ajpf-645	80	9	sequence	sequence	NOUN
ajpf-645	80	10	(	(	PUNCT
ajpf-645	80	11	ss	ss	NOUN
ajpf-645	80	12	)	)	PUNCT
ajpf-645	80	13	dna	dna	PROPN
ajpf-645	80	14	.	.	PUNCT
ajpf-645	81	1	the	the	DET
ajpf-645	81	2	signal	signal	ADJ
ajpf-645	81	3	sequences	sequence	NOUN
ajpf-645	81	4	of	of	ADP
ajpf-645	81	5	bla	bla	NOUN
ajpf-645	81	6	(	(	PUNCT
ajpf-645	81	7	–lactamase	–lactamase	NOUN
ajpf-645	81	8	)	)	PUNCT
ajpf-645	81	9	,	,	PUNCT
ajpf-645	81	10	agfa	agfa	PROPN
ajpf-645	81	11	(	(	PUNCT
ajpf-645	81	12	thin	thin	PROPN
ajpf-645	81	13	aggregative	aggregative	ADJ
ajpf-645	81	14	fimbriae	fimbriae	NOUN
ajpf-645	81	15	a	a	X
ajpf-645	81	16	)	)	PUNCT
ajpf-645	81	17	,	,	PUNCT
ajpf-645	81	18	stfa	stfa	PROPN
ajpf-645	81	19	s.	s.	PROPN
ajpf-645	81	20	typhimurium	typhimurium	PROPN
ajpf-645	81	21	fimbriae	fimbriae	VERB
ajpf-645	81	22	a	a	NOUN
ajpf-645	81	23	)	)	PUNCT
ajpf-645	81	24	and	and	CCONJ
ajpf-645	81	25	ompw	ompw	NOUN
ajpf-645	81	26	(	(	PUNCT
ajpf-645	81	27	outer	outer	ADJ
ajpf-645	81	28	membrane	membrane	NOUN
ajpf-645	81	29	protein	protein	NOUN
ajpf-645	81	30	w	w	NOUN
ajpf-645	81	31	)	)	PUNCT
ajpf-645	81	32	were	be	AUX
ajpf-645	81	33	used	use	VERB
ajpf-645	81	34	in	in	ADP
ajpf-645	81	35	this	this	DET
ajpf-645	81	36	study	study	NOUN
ajpf-645	81	37	.	.	PUNCT
ajpf-645	82	1	each	each	DET
ajpf-645	82	2	pcramplified	pcramplifie	VERB
ajpf-645	82	3	bsphi	bsphi	NOUN
ajpf-645	82	4	-	-	PUNCT
ajpf-645	82	5	eco	eco	NOUN
ajpf-645	82	6	ri	ri	NOUN
ajpf-645	82	7	ss	ss	PROPN
ajpf-645	82	8	dna	dna	PROPN
ajpf-645	82	9	was	be	AUX
ajpf-645	82	10	cloned	clone	VERB
ajpf-645	82	11	into	into	ADP
ajpf-645	82	12	pya3342	pya3342	NOUN
ajpf-645	82	13	digested	digest	VERB
ajpf-645	82	14	with	with	ADP
ajpf-645	82	15	ncoi	ncoi	NOUN
ajpf-645	82	16	and	and	CCONJ
ajpf-645	82	17	ecori	ecori	PROPN
ajpf-645	82	18	.	.	PUNCT
ajpf-645	83	1	gray	gray	PROPN
ajpf-645	83	2	box	box	PROPN
ajpf-645	83	3	indicates	indicate	VERB
ajpf-645	83	4	a	a	DET
ajpf-645	83	5	his6	his6	NOUN
ajpf-645	83	6	-	-	PUNCT
ajpf-645	83	7	tag	tag	NOUN
ajpf-645	83	8	contained	contain	VERB
ajpf-645	83	9	at	at	ADP
ajpf-645	83	10	the	the	DET
ajpf-645	83	11	c	c	NOUN
ajpf-645	83	12	-	-	PUNCT
ajpf-645	83	13	terminus	terminus	NOUN
ajpf-645	83	14	of	of	ADP
ajpf-645	83	15	each	each	DET
ajpf-645	83	16	ss	ss	PROPN
ajpf-645	83	17	.	.	PROPN
ajpf-645	83	18	exhibited	exhibit	VERB
ajpf-645	83	19	his6	his6	NOUN
ajpf-645	83	20	residues	residue	NOUN
ajpf-645	83	21	.	.	PUNCT
ajpf-645	84	1	it	it	PRON
ajpf-645	84	2	is	be	AUX
ajpf-645	84	3	possible	possible	ADJ
ajpf-645	84	4	to	to	PART
ajpf-645	84	5	speculate	speculate	VERB
ajpf-645	84	6	that	that	SCONJ
ajpf-645	84	7	expression	expression	NOUN
ajpf-645	84	8	of	of	ADP
ajpf-645	84	9	each	each	DET
ajpf-645	84	10	ss	ss	NOUN
ajpf-645	84	11	and	and	CCONJ
ajpf-645	84	12	his6	his6	NOUN
ajpf-645	84	13	coding	code	VERB
ajpf-645	84	14	residue	residue	NOUN
ajpf-645	84	15	were	be	AUX
ajpf-645	84	16	regulated	regulate	VERB
ajpf-645	84	17	by	by	ADP
ajpf-645	84	18	ptrc	ptrc	NOUN
ajpf-645	84	19	promoter	promoter	NOUN
ajpf-645	84	20	.	.	PUNCT
ajpf-645	85	1	a	a	DET
ajpf-645	85	2	strategy	strategy	NOUN
ajpf-645	85	3	for	for	ADP
ajpf-645	85	4	preparation	preparation	NOUN
ajpf-645	85	5	of	of	ADP
ajpf-645	85	6	these	these	DET
ajpf-645	85	7	vectors	vector	NOUN
ajpf-645	85	8	is	be	AUX
ajpf-645	85	9	shown	show	VERB
ajpf-645	85	10	in	in	ADP
ajpf-645	85	11	figure	figure	NOUN
ajpf-645	85	12	1	1	NUM
ajpf-645	85	13	.	.	PUNCT
ajpf-645	86	1	in	in	ADP
ajpf-645	86	2	e.	e.	PROPN
ajpf-645	86	3	coli	coli	PROPN
ajpf-645	86	4	,	,	PUNCT
ajpf-645	86	5	the	the	DET
ajpf-645	86	6	lactamase	lactamase	NOUN
ajpf-645	86	7	is	be	AUX
ajpf-645	86	8	secreted	secrete	VERB
ajpf-645	86	9	into	into	ADP
ajpf-645	86	10	periplasmic	periplasmic	ADJ
ajpf-645	86	11	space	space	NOUN
ajpf-645	86	12	through	through	ADP
ajpf-645	86	13	type	type	PROPN
ajpf-645	86	14	ii	ii	PROPN
ajpf-645	86	15	sec	sec	PROPN
ajpf-645	86	16	-	-	PUNCT
ajpf-645	86	17	dependent	dependent	ADJ
ajpf-645	86	18	secretion	secretion	NOUN
ajpf-645	86	19	system	system	NOUN
ajpf-645	86	20	,	,	PUNCT
ajpf-645	86	21	but	but	CCONJ
ajpf-645	86	22	in	in	ADP
ajpf-645	86	23	independent	independent	ADJ
ajpf-645	86	24	manner	manner	NOUN
ajpf-645	86	25	of	of	ADP
ajpf-645	86	26	secb	secb	NOUN
ajpf-645	86	27	(	(	PUNCT
ajpf-645	86	28	beha	beha	NOUN
ajpf-645	86	29	et	et	NOUN
ajpf-645	86	30	al	al	PROPN
ajpf-645	86	31	.	.	PROPN
ajpf-645	86	32	,	,	PUNCT
ajpf-645	86	33	2003	2003	NUM
ajpf-645	86	34	)	)	PUNCT
ajpf-645	86	35	.	.	PUNCT
ajpf-645	87	1	agfa	agfa	PROPN
ajpf-645	87	2	and	and	CCONJ
ajpf-645	87	3	stfa	stfa	PROPN
ajpf-645	87	4	,	,	PUNCT
ajpf-645	87	5	major	major	ADJ
ajpf-645	87	6	fimbrial	fimbrial	ADJ
ajpf-645	87	7	proteins	protein	NOUN
ajpf-645	87	8	of	of	ADP
ajpf-645	87	9	s.	s.	PROPN
ajpf-645	87	10	typhimurium	typhimurium	PROPN
ajpf-645	87	11	,	,	PUNCT
ajpf-645	87	12	agf	agf	PROPN
ajpf-645	87	13	and	and	CCONJ
ajpf-645	87	14	stf	stf	PROPN
ajpf-645	87	15	fimbriae	fimbriae	NOUN
ajpf-645	87	16	are	be	AUX
ajpf-645	87	17	secreted	secrete	VERB
ajpf-645	87	18	into	into	ADP
ajpf-645	87	19	extracellular	extracellular	ADJ
ajpf-645	87	20	matrix	matrix	NOUN
ajpf-645	87	21	by	by	ADP
ajpf-645	87	22	type	type	NOUN
ajpf-645	87	23	iv	iv	NUM
ajpf-645	87	24	sec	sec	PROPN
ajpf-645	87	25	-	-	PUNCT
ajpf-645	87	26	dependent	dependent	ADJ
ajpf-645	87	27	secretion	secretion	NOUN
ajpf-645	87	28	system	system	NOUN
ajpf-645	87	29	.	.	PUNCT
ajpf-645	88	1	the	the	DET
ajpf-645	88	2	mechanism	mechanism	NOUN
ajpf-645	88	3	of	of	ADP
ajpf-645	88	4	agf	agf	PROPN
ajpf-645	88	5	fimbrial	fimbrial	ADJ
ajpf-645	88	6	protein	protein	NOUN
ajpf-645	88	7	secretion	secretion	NOUN
ajpf-645	88	8	is	be	AUX
ajpf-645	88	9	unknown	unknown	ADJ
ajpf-645	88	10	,	,	PUNCT
ajpf-645	88	11	whereas	whereas	SCONJ
ajpf-645	88	12	stf	stf	PROPN
ajpf-645	88	13	fimbrial	fimbrial	ADJ
ajpf-645	88	14	proteins	protein	NOUN
ajpf-645	88	15	are	be	AUX
ajpf-645	88	16	secreted	secrete	VERB
ajpf-645	88	17	by	by	ADP
ajpf-645	88	18	similar	similar	ADJ
ajpf-645	88	19	manner	manner	NOUN
ajpf-645	88	20	to	to	PART
ajpf-645	88	21	type	type	VERB
ajpf-645	88	22	ii	ii	PROPN
ajpf-645	88	23	sec	sec	PROPN
ajpf-645	88	24	-	-	PUNCT
ajpf-645	88	25	dependent	dependent	ADJ
ajpf-645	88	26	pathway	pathway	NOUN
ajpf-645	88	27	(	(	PUNCT
ajpf-645	88	28	humphries	humphrie	NOUN
ajpf-645	88	29	et	et	PROPN
ajpf-645	88	30	al	al	PROPN
ajpf-645	88	31	.	.	PROPN
ajpf-645	88	32	,	,	PUNCT
ajpf-645	88	33	2003	2003	NUM
ajpf-645	88	34	)	)	PUNCT
ajpf-645	88	35	.	.	PUNCT
ajpf-645	89	1	the	the	DET
ajpf-645	89	2	ompw	ompw	NOUN
ajpf-645	89	3	(	(	PUNCT
ajpf-645	89	4	outer	outer	ADJ
ajpf-645	89	5	membrane	membrane	NOUN
ajpf-645	89	6	protein	protein	NOUN
ajpf-645	89	7	w	w	NOUN
ajpf-645	89	8	)	)	PUNCT
ajpf-645	89	9	is	be	AUX
ajpf-645	89	10	translocated	translocate	VERB
ajpf-645	89	11	through	through	ADP
ajpf-645	89	12	type	type	PROPN
ajpf-645	89	13	ii	ii	PROPN
ajpf-645	89	14	secand	secand	PROPN
ajpf-645	89	15	secbdependent	secbdependent	NOUN
ajpf-645	89	16	pathway	pathway	NOUN
ajpf-645	89	17	(	(	PUNCT
ajpf-645	89	18	driessen	driessen	PROPN
ajpf-645	89	19	et	et	PROPN
ajpf-645	89	20	al	al	PROPN
ajpf-645	89	21	.	.	PROPN
ajpf-645	89	22	,	,	PUNCT
ajpf-645	89	23	2001	2001	NUM
ajpf-645	89	24	;	;	PUNCT
ajpf-645	89	25	koch	koch	PROPN
ajpf-645	89	26	et	et	PROPN
ajpf-645	89	27	al	al	PROPN
ajpf-645	89	28	.	.	PROPN
ajpf-645	89	29	,	,	PUNCT
ajpf-645	89	30	2003	2003	NUM
ajpf-645	89	31	)	)	PUNCT
ajpf-645	89	32	.	.	PUNCT
ajpf-645	90	1	identification	identification	NOUN
ajpf-645	90	2	of	of	ADP
ajpf-645	90	3	foreign	foreign	ADJ
ajpf-645	90	4	antigen	antigen	ADJ
ajpf-645	90	5	expression	expression	NOUN
ajpf-645	90	6	by	by	ADP
ajpf-645	90	7	pspa	pspa	NOUN
ajpf-645	90	8	protein	protein	NOUN
ajpf-645	90	9	the	the	DET
ajpf-645	90	10	pspa	pspa	ADJ
ajpf-645	90	11	protein	protein	NOUN
ajpf-645	90	12	(	(	PUNCT
ajpf-645	90	13	streptococcus	streptococcus	VERB
ajpf-645	90	14	pneumoniae	pneumoniae	ADJ
ajpf-645	90	15	surface	surface	NOUN
ajpf-645	90	16	protein	protein	NOUN
ajpf-645	90	17	a	a	NOUN
ajpf-645	90	18	)	)	PUNCT
ajpf-645	90	19	was	be	AUX
ajpf-645	90	20	selected	select	VERB
ajpf-645	90	21	as	as	ADP
ajpf-645	90	22	a	a	DET
ajpf-645	90	23	testing	testing	NOUN
ajpf-645	90	24	protein	protein	NOUN
ajpf-645	90	25	,	,	PUNCT
ajpf-645	90	26	for	for	SCONJ
ajpf-645	90	27	protein	protein	NOUN
ajpf-645	90	28	secretion	secretion	NOUN
ajpf-645	90	29	through	through	ADP
ajpf-645	90	30	the	the	DET
ajpf-645	90	31	system	system	NOUN
ajpf-645	90	32	developed	develop	VERB
ajpf-645	90	33	in	in	ADP
ajpf-645	90	34	this	this	DET
ajpf-645	90	35	study	study	NOUN
ajpf-645	90	36	(	(	PUNCT
ajpf-645	90	37	briles	brile	NOUN
ajpf-645	90	38	et	et	PROPN
ajpf-645	90	39	al	al	PROPN
ajpf-645	90	40	.	.	PROPN
ajpf-645	90	41	,	,	PUNCT
ajpf-645	90	42	1998	1998	NUM
ajpf-645	90	43	)	)	PUNCT
ajpf-645	90	44	.	.	PUNCT
ajpf-645	91	1	a	a	DET
ajpf-645	91	2	765	765	NUM
ajpf-645	91	3	bp	bp	PROPN
ajpf-645	91	4	ecorihindiii	ecorihindiii	NOUN
ajpf-645	91	5	pspa	pspa	ADJ
ajpf-645	91	6	gene	gene	NOUN
ajpf-645	91	7	isolated	isolate	VERB
ajpf-645	91	8	from	from	ADP
ajpf-645	91	9	pya3494	pya3494	NOUN
ajpf-645	91	10	was	be	AUX
ajpf-645	91	11	ligated	ligate	VERB
ajpf-645	91	12	with	with	ADP
ajpf-645	91	13	the	the	DET
ajpf-645	91	14	plasmids	plasmid	NOUN
ajpf-645	91	15	pmmp62	pmmp62	NOUN
ajpf-645	91	16	,	,	PUNCT
ajpf-645	91	17	pmmp63	pmmp63	ADJ
ajpf-645	91	18	,	,	PUNCT
ajpf-645	91	19	pmmp64	pmmp64	ADJ
ajpf-645	91	20	or	or	CCONJ
ajpf-645	91	21	pmmp65	pmmp65	ADV
ajpf-645	91	22	digested	digest	VERB
ajpf-645	91	23	with	with	ADP
ajpf-645	91	24	ecori	ecori	NOUN
ajpf-645	91	25	and	and	CCONJ
ajpf-645	91	26	hindiii	hindiii	ADJ
ajpf-645	91	27	(	(	PUNCT
ajpf-645	91	28	figure	figure	NOUN
ajpf-645	91	29	2	2	NUM
ajpf-645	91	30	)	)	PUNCT
ajpf-645	91	31	.	.	PUNCT
ajpf-645	92	1	the	the	DET
ajpf-645	92	2	recombinant	recombinant	ADJ
ajpf-645	92	3	dnas	dna	NOUN
ajpf-645	92	4	were	be	AUX
ajpf-645	92	5	transformed	transform	VERB
ajpf-645	92	6	into	into	ADP
ajpf-645	92	7	e.	e.	PROPN
ajpf-645	92	8	coli	coli	PROPN
ajpf-645	92	9	x6212	x6212	PROPN
ajpf-645	93	1	(	(	PUNCT
ajpf-645	93	2	kang	kang	PROPN
ajpf-645	93	3	et	et	PROPN
ajpf-645	93	4	al	al	PROPN
ajpf-645	93	5	.	.	PROPN
ajpf-645	93	6	,	,	PUNCT
ajpf-645	93	7	2002	2002	NUM
ajpf-645	93	8	)	)	PUNCT
ajpf-645	93	9	.	.	PUNCT
ajpf-645	94	1	furthermore	furthermore	ADV
ajpf-645	94	2	,	,	PUNCT
ajpf-645	94	3	the	the	DET
ajpf-645	94	4	presence	presence	NOUN
ajpf-645	94	5	of	of	ADP
ajpf-645	94	6	recombinant	recombinant	ADJ
ajpf-645	94	7	plasmids	plasmid	NOUN
ajpf-645	94	8	were	be	AUX
ajpf-645	94	9	confirmed	confirm	VERB
ajpf-645	94	10	by	by	ADP
ajpf-645	94	11	analyses	analysis	NOUN
ajpf-645	94	12	of	of	ADP
ajpf-645	94	13	restriction	restriction	NOUN
ajpf-645	94	14	enzyme	enzyme	NOUN
ajpf-645	94	15	digestion	digestion	NOUN
ajpf-645	94	16	figure	figure	NOUN
ajpf-645	94	17	2	2	NUM
ajpf-645	94	18	.	.	PUNCT
ajpf-645	94	19	genetic	genetic	ADJ
ajpf-645	94	20	maps	map	NOUN
ajpf-645	94	21	of	of	ADP
ajpf-645	94	22	recombinant	recombinant	ADJ
ajpf-645	94	23	plasmids	plasmid	NOUN
ajpf-645	94	24	.	.	PUNCT
ajpf-645	95	1	the	the	DET
ajpf-645	95	2	physical	physical	ADJ
ajpf-645	95	3	maps	map	NOUN
ajpf-645	95	4	of	of	ADP
ajpf-645	95	5	each	each	DET
ajpf-645	95	6	recombinant	recombinant	ADJ
ajpf-645	95	7	plasmid	plasmid	NOUN
ajpf-645	95	8	are	be	AUX
ajpf-645	95	9	indicated	indicate	VERB
ajpf-645	95	10	with	with	ADP
ajpf-645	95	11	their	their	PRON
ajpf-645	95	12	representative	representative	ADJ
ajpf-645	95	13	restriction	restriction	NOUN
ajpf-645	95	14	enzyme	enzyme	NOUN
ajpf-645	95	15	sites	site	NOUN
ajpf-645	95	16	:	:	PUNCT
ajpf-645	95	17	(	(	PUNCT
ajpf-645	95	18	a	a	X
ajpf-645	95	19	)	)	PUNCT
ajpf-645	95	20	basal	basal	PROPN
ajpf-645	95	21	vector	vector	NOUN
ajpf-645	95	22	,	,	PUNCT
ajpf-645	95	23	(	(	PUNCT
ajpf-645	95	24	b	b	NOUN
ajpf-645	95	25	)	)	PUNCT
ajpf-645	95	26	pmmp62	pmmp62	NOUN
ajpf-645	95	27	,	,	PUNCT
ajpf-645	95	28	(	(	PUNCT
ajpf-645	95	29	c	c	NOUN
ajpf-645	95	30	)	)	PUNCT
ajpf-645	95	31	pmmp63	pmmp63	ADV
ajpf-645	95	32	,	,	PUNCT
ajpf-645	95	33	(	(	PUNCT
ajpf-645	95	34	d	d	NOUN
ajpf-645	95	35	)	)	PUNCT
ajpf-645	95	36	pmmp64	pmmp64	NOUN
ajpf-645	95	37	and	and	CCONJ
ajpf-645	95	38	(	(	PUNCT
ajpf-645	95	39	e	e	NOUN
ajpf-645	95	40	)	)	PUNCT
ajpf-645	95	41	pmmp65	pmmp65	NOUN
ajpf-645	95	42	.	.	PUNCT
ajpf-645	96	1	the	the	DET
ajpf-645	96	2	ptrc	ptrc	PROPN
ajpf-645	96	3	promoter	promoter	NOUN
ajpf-645	96	4	region	region	NOUN
ajpf-645	96	5	,	,	PUNCT
ajpf-645	96	6	ribosome	ribosome	ADJ
ajpf-645	96	7	binding	bind	VERB
ajpf-645	96	8	site	site	NOUN
ajpf-645	96	9	(	(	PUNCT
ajpf-645	96	10	rbs	rbs	PROPN
ajpf-645	96	11	)	)	PUNCT
ajpf-645	96	12	,	,	PUNCT
ajpf-645	96	13	each	each	DET
ajpf-645	96	14	ss	ss	PROPN
ajpf-645	96	15	,	,	PUNCT
ajpf-645	96	16	his6	his6	NOUN
ajpf-645	96	17	-	-	PUNCT
ajpf-645	96	18	epitope	epitope	PROPN
ajpf-645	96	19	and	and	CCONJ
ajpf-645	96	20	multi	multi	ADJ
ajpf-645	96	21	-	-	ADJ
ajpf-645	96	22	cloning	cloning	ADJ
ajpf-645	96	23	sites	site	NOUN
ajpf-645	96	24	(	(	PUNCT
ajpf-645	96	25	mcs	mcs	NOUN
ajpf-645	96	26	)	)	PUNCT
ajpf-645	96	27	are	be	AUX
ajpf-645	96	28	revealed	reveal	VERB
ajpf-645	96	29	in	in	ADP
ajpf-645	96	30	enlarged	enlarged	ADJ
ajpf-645	96	31	box	box	PROPN
ajpf-645	96	32	.	.	PUNCT
ajpf-645	97	1	unique	unique	ADJ
ajpf-645	97	2	enzyme	enzyme	NOUN
ajpf-645	97	3	sites	site	NOUN
ajpf-645	97	4	in	in	ADP
ajpf-645	97	5	mcs	mcs	PROPN
ajpf-645	97	6	are	be	AUX
ajpf-645	97	7	designated	designate	VERB
ajpf-645	97	8	by	by	ADP
ajpf-645	97	9	underlined	underline	VERB
ajpf-645	97	10	bold	bold	ADJ
ajpf-645	97	11	letters	letter	NOUN
ajpf-645	97	12	.	.	PUNCT
ajpf-645	98	1	ptrc	ptrc	PROPN
ajpf-645	98	2	,	,	PUNCT
ajpf-645	98	3	trc	trc	PROPN
ajpf-645	98	4	promoter	promoter	NOUN
ajpf-645	98	5	;	;	PUNCT
ajpf-645	98	6	5st1t2	5st1t2	NUM
ajpf-645	98	7	,	,	PUNCT
ajpf-645	98	8	5s	5s	PROPN
ajpf-645	98	9	rrna	rrna	NOUN
ajpf-645	98	10	terminator	terminator	NOUN
ajpf-645	98	11	;	;	PUNCT
ajpf-645	98	12	“	"	PUNCT
ajpf-645	98	13	asd	asd	NOUN
ajpf-645	98	14	”	"	PUNCT
ajpf-645	98	15	,	,	PUNCT
ajpf-645	98	16	aspartate	aspartate	NOUN
ajpf-645	98	17	semialdehyde	semialdehyde	ADJ
ajpf-645	98	18	dehydrogenase	dehydrogenase	NOUN
ajpf-645	98	19	gene	gene	NOUN
ajpf-645	98	20	;	;	PUNCT
ajpf-645	98	21	ss	ss	NOUN
ajpf-645	98	22	,	,	PUNCT
ajpf-645	98	23	ompw	ompw	NOUN
ajpf-645	98	24	ss	ss	PROPN
ajpf-645	98	25	(	(	PUNCT
ajpf-645	98	26	b	b	NOUN
ajpf-645	98	27	)	)	PUNCT
ajpf-645	98	28	,	,	PUNCT
ajpf-645	98	29	stfa	stfa	VERB
ajpf-645	98	30	ss	ss	INTJ
ajpf-645	98	31	(	(	PUNCT
ajpf-645	98	32	c	c	NOUN
ajpf-645	98	33	)	)	PUNCT
ajpf-645	98	34	,	,	PUNCT
ajpf-645	98	35	bla	bla	NOUN
ajpf-645	98	36	ss	ss	PROPN
ajpf-645	98	37	(	(	PUNCT
ajpf-645	98	38	d	d	NOUN
ajpf-645	98	39	)	)	PUNCT
ajpf-645	98	40	and	and	CCONJ
ajpf-645	98	41	agfa	agfa	PROPN
ajpf-645	98	42	ss	ss	PROPN
ajpf-645	98	43	(	(	PUNCT
ajpf-645	98	44	e	e	NOUN
ajpf-645	98	45	)	)	PUNCT
ajpf-645	98	46	.	.	PUNCT
ajpf-645	99	1	and	and	CCONJ
ajpf-645	99	2	nucleotide	nucleotide	VERB
ajpf-645	99	3	sequencing	sequencing	NOUN
ajpf-645	99	4	,	,	PUNCT
ajpf-645	99	5	and	and	CCONJ
ajpf-645	99	6	were	be	AUX
ajpf-645	99	7	finally	finally	ADV
ajpf-645	99	8	designed	design	VERB
ajpf-645	99	9	as	as	ADP
ajpf-645	99	10	pmmp66	pmmp66	NOUN
ajpf-645	99	11	,	,	PUNCT
ajpf-645	99	12	67	67	NUM
ajpf-645	99	13	,	,	PUNCT
ajpf-645	99	14	68	68	NUM
ajpf-645	99	15	and	and	CCONJ
ajpf-645	99	16	70	70	NUM
ajpf-645	99	17	(	(	PUNCT
ajpf-645	99	18	table	table	NOUN
ajpf-645	99	19	1	1	NUM
ajpf-645	99	20	)	)	PUNCT
ajpf-645	99	21	.	.	PUNCT
ajpf-645	100	1	the	the	DET
ajpf-645	100	2	level	level	NOUN
ajpf-645	100	3	of	of	ADP
ajpf-645	100	4	expressed	express	VERB
ajpf-645	100	5	pspa	pspa	ADJ
ajpf-645	100	6	protein	protein	NOUN
ajpf-645	100	7	in	in	ADP
ajpf-645	100	8	the	the	DET
ajpf-645	100	9	s.	s.	PROPN
ajpf-645	100	10	typhimuium	typhimuium	NOUN
ajpf-645	100	11	8554	8554	PROPN
ajpf-645	100	12	host	host	NOUN
ajpf-645	100	13	was	be	AUX
ajpf-645	100	14	examined	examine	VERB
ajpf-645	100	15	by	by	ADP
ajpf-645	100	16	immunoblotting	immunoblotte	VERB
ajpf-645	100	17	.	.	PUNCT
ajpf-645	101	1	a	a	DET
ajpf-645	101	2	monoclonal	monoclonal	NOUN
ajpf-645	101	3	antibody	antibody	NOUN
ajpf-645	101	4	xi126	xi126	PROPN
ajpf-645	101	5	(	(	PUNCT
ajpf-645	101	6	mcdaniel	mcdaniel	PROPN
ajpf-645	101	7	et	et	PROPN
ajpf-645	101	8	al	al	PROPN
ajpf-645	101	9	.	.	PROPN
ajpf-645	101	10	,	,	PUNCT
ajpf-645	101	11	1984	1984	NUM
ajpf-645	101	12	)	)	PUNCT
ajpf-645	101	13	was	be	AUX
ajpf-645	101	14	used	use	VERB
ajpf-645	101	15	to	to	PART
ajpf-645	101	16	detect	detect	VERB
ajpf-645	101	17	pspa	pspa	ADJ
ajpf-645	101	18	protein	protein	NOUN
ajpf-645	101	19	specifically	specifically	ADV
ajpf-645	101	20	.	.	PUNCT
ajpf-645	102	1	an	an	DET
ajpf-645	102	2	anti	anti	ADJ
ajpf-645	102	3	-	-	ADJ
ajpf-645	102	4	his6	his6	ADJ
ajpf-645	102	5	-	-	PUNCT
ajpf-645	102	6	epitope	epitope	NOUN
ajpf-645	102	7	antibody	antibody	NOUN
ajpf-645	102	8	(	(	PUNCT
ajpf-645	102	9	santacruz	santacruz	PROPN
ajpf-645	102	10	biotechnology	biotechnology	PROPN
ajpf-645	102	11	,	,	PUNCT
ajpf-645	102	12	inc	inc	PROPN
ajpf-645	102	13	.	.	PROPN
ajpf-645	102	14	)	)	PUNCT
ajpf-645	102	15	raised	raise	VERB
ajpf-645	102	16	in	in	ADP
ajpf-645	102	17	mouse	mouse	NOUN
ajpf-645	102	18	was	be	AUX
ajpf-645	102	19	used	use	VERB
ajpf-645	102	20	to	to	PART
ajpf-645	102	21	detect	detect	VERB
ajpf-645	102	22	expressed	express	VERB
ajpf-645	102	23	protein	protein	NOUN
ajpf-645	102	24	against	against	ADP
ajpf-645	102	25	his6	his6	NOUN
ajpf-645	102	26	-	-	PUNCT
ajpf-645	102	27	tagged	tag	VERB
ajpf-645	102	28	signal	signal	NOUN
ajpf-645	102	29	peptide	peptide	NOUN
ajpf-645	102	30	.	.	PUNCT
ajpf-645	103	1	an	an	DET
ajpf-645	103	2	anti	anti	ADJ
ajpf-645	103	3	-	-	ADJ
ajpf-645	103	4	mouse	mouse	ADJ
ajpf-645	103	5	igg	igg	NOUN
ajpf-645	103	6	conjugated	conjugate	VERB
ajpf-645	103	7	with	with	ADP
ajpf-645	103	8	hrp	hrp	PROPN
ajpf-645	103	9	(	(	PUNCT
ajpf-645	103	10	horseradish	horseradish	ADJ
ajpf-645	103	11	peroxidase	peroxidase	NOUN
ajpf-645	103	12	)	)	PUNCT
ajpf-645	103	13	(	(	PUNCT
ajpf-645	103	14	stressgen	stressgen	PROPN
ajpf-645	103	15	co.	co.	PROPN
ajpf-645	103	16	)	)	PUNCT
ajpf-645	103	17	obtained	obtain	VERB
ajpf-645	103	18	from	from	ADP
ajpf-645	103	19	goat	goat	PROPN
ajpf-645	103	20	was	be	AUX
ajpf-645	103	21	used	use	VERB
ajpf-645	103	22	as	as	ADP
ajpf-645	103	23	a	a	DET
ajpf-645	103	24	secondary	secondary	ADJ
ajpf-645	103	25	antibodies	antibody	NOUN
ajpf-645	103	26	to	to	PART
ajpf-645	103	27	recognize	recognize	VERB
ajpf-645	103	28	primary	primary	ADJ
ajpf-645	103	29	antibodies	antibody	NOUN
ajpf-645	103	30	.	.	PUNCT
ajpf-645	104	1	the	the	DET
ajpf-645	104	2	s.	s.	PROPN
ajpf-645	104	3	typhimuium	typhimuium	NOUN
ajpf-645	104	4	8554	8554	PROPN
ajpf-645	104	5	carrying	carry	VERB
ajpf-645	104	6	pya3494	pya3494	NOUN
ajpf-645	104	7	,	,	PUNCT
ajpf-645	104	8	pmmp66	pmmp66	NOUN
ajpf-645	104	9	,	,	PUNCT
ajpf-645	104	10	pmmp67	pmmp67	PROPN
ajpf-645	104	11	,	,	PUNCT
ajpf-645	104	12	pmmp68	pmmp68	ADJ
ajpf-645	104	13	or	or	CCONJ
ajpf-645	104	14	pmmp70	pmmp70	NOUN
ajpf-645	104	15	were	be	AUX
ajpf-645	104	16	cultured	cultured	ADJ
ajpf-645	104	17	to	to	ADP
ajpf-645	104	18	od600	od600	ADP
ajpf-645	104	19	0.8	0.8	NUM
ajpf-645	104	20	in	in	ADP
ajpf-645	104	21	lb	lb	DET
ajpf-645	104	22	broth	broth	NOUN
ajpf-645	104	23	at	at	ADP
ajpf-645	104	24	37	37	NUM
ajpf-645	104	25	°	°	NOUN
ajpf-645	104	26	c	c	NOUN
ajpf-645	104	27	.	.	PUNCT
ajpf-645	105	1	total	total	ADJ
ajpf-645	105	2	cell	cell	NOUN
ajpf-645	105	3	lysate	lysate	NOUN
ajpf-645	105	4	of	of	ADP
ajpf-645	105	5	each	each	DET
ajpf-645	105	6	sample	sample	NOUN
ajpf-645	105	7	containing	contain	VERB
ajpf-645	105	8	65	65	NUM
ajpf-645	105	9	µg	µg	NOUN
ajpf-645	105	10	of	of	ADP
ajpf-645	105	11	protein	protein	NOUN
ajpf-645	105	12	was	be	AUX
ajpf-645	105	13	subjected	subject	VERB
ajpf-645	105	14	for	for	ADP
ajpf-645	105	15	sds	sds	NOUN
ajpf-645	105	16	-	-	PUNCT
ajpf-645	105	17	page	page	NOUN
ajpf-645	105	18	analysis	analysis	NOUN
ajpf-645	105	19	.	.	PUNCT
ajpf-645	106	1	as	as	SCONJ
ajpf-645	106	2	seen	see	VERB
ajpf-645	106	3	in	in	ADP
ajpf-645	106	4	figure	figure	NOUN
ajpf-645	106	5	3	3	NUM
ajpf-645	106	6	,	,	PUNCT
ajpf-645	106	7	all	all	DET
ajpf-645	106	8	samples	sample	NOUN
ajpf-645	106	9	exhibited	exhibit	VERB
ajpf-645	106	10	a	a	DET
ajpf-645	106	11	major	major	ADJ
ajpf-645	106	12	protein	protein	NOUN
ajpf-645	106	13	bands	band	NOUN
ajpf-645	106	14	with	with	ADP
ajpf-645	106	15	approximately	approximately	ADV
ajpf-645	106	16	37	37	NUM
ajpf-645	106	17	kda	kda	NOUN
ajpf-645	106	18	size	size	NOUN
ajpf-645	106	19	.	.	PUNCT
ajpf-645	107	1	a	a	DET
ajpf-645	107	2	little	little	ADJ
ajpf-645	107	3	size	size	NOUN
ajpf-645	107	4	differences	difference	NOUN
ajpf-645	107	5	of	of	ADP
ajpf-645	107	6	the	the	DET
ajpf-645	107	7	figure	figure	NOUN
ajpf-645	107	8	3	3	NUM
ajpf-645	107	9	.	.	PUNCT
ajpf-645	107	10	expression	expression	NOUN
ajpf-645	107	11	of	of	ADP
ajpf-645	107	12	pspa	pspa	NOUN
ajpf-645	107	13	in	in	ADP
ajpf-645	107	14	s.	s.	PROPN
ajpf-645	107	15	typhimurium	typhimurium	NOUN
ajpf-645	107	16	containing	contain	VERB
ajpf-645	107	17	recombinant	recombinant	ADJ
ajpf-645	107	18	plasmids	plasmid	NOUN
ajpf-645	107	19	.	.	PUNCT
ajpf-645	108	1	s.	s.	PROPN
ajpf-645	108	2	typhimurium	typhimurium	PROPN
ajpf-645	108	3	strains	strain	VERB
ajpf-645	108	4	harboring	harboring	NOUN
ajpf-645	108	5	pya3494	pya3494	NOUN
ajpf-645	108	6	,	,	PUNCT
ajpf-645	108	7	pmmp66	pmmp66	NOUN
ajpf-645	108	8	,	,	PUNCT
ajpf-645	108	9	67	67	NUM
ajpf-645	108	10	,	,	PUNCT
ajpf-645	108	11	68	68	NUM
ajpf-645	108	12	and	and	CCONJ
ajpf-645	108	13	70	70	NUM
ajpf-645	108	14	were	be	AUX
ajpf-645	108	15	grown	grow	VERB
ajpf-645	108	16	in	in	ADP
ajpf-645	108	17	lb	lb	DET
ajpf-645	108	18	broth	broth	NOUN
ajpf-645	108	19	until	until	ADP
ajpf-645	108	20	exponential	exponential	ADJ
ajpf-645	108	21	phase	phase	NOUN
ajpf-645	108	22	(	(	PUNCT
ajpf-645	108	23	0.8	0.8	NUM
ajpf-645	108	24	at	at	ADP
ajpf-645	108	25	od600	od600	NOUN
ajpf-645	108	26	)	)	PUNCT
ajpf-645	108	27	.	.	PUNCT
ajpf-645	109	1	equal	equal	ADJ
ajpf-645	109	2	amounts	amount	NOUN
ajpf-645	109	3	of	of	ADP
ajpf-645	109	4	cell	cell	NOUN
ajpf-645	109	5	lysates	lysate	NOUN
ajpf-645	109	6	were	be	AUX
ajpf-645	109	7	subjected	subject	VERB
ajpf-645	109	8	to	to	ADP
ajpf-645	109	9	sds	sds	NOUN
ajpf-645	109	10	-	-	PUNCT
ajpf-645	109	11	page	page	NOUN
ajpf-645	109	12	followed	follow	VERB
ajpf-645	109	13	by	by	ADP
ajpf-645	109	14	immunoblot	immunoblot	NOUN
ajpf-645	109	15	analyses	analysis	NOUN
ajpf-645	109	16	with	with	ADP
ajpf-645	109	17	specific	specific	ADJ
ajpf-645	109	18	antibodies	antibody	NOUN
ajpf-645	109	19	for	for	ADP
ajpf-645	109	20	each	each	DET
ajpf-645	109	21	component	component	NOUN
ajpf-645	109	22	.	.	PUNCT
ajpf-645	110	1	the	the	PRON
ajpf-645	110	2	goat	goat	PROPN
ajpf-645	110	3	anti	anti	PROPN
ajpf-645	110	4	-	-	NOUN
ajpf-645	110	5	mouse	mouse	NOUN
ajpf-645	110	6	conjugated	conjugate	VERB
ajpf-645	110	7	with	with	ADP
ajpf-645	110	8	hrp	hrp	PROPN
ajpf-645	110	9	was	be	AUX
ajpf-645	110	10	used	use	VERB
ajpf-645	110	11	as	as	ADP
ajpf-645	110	12	the	the	DET
ajpf-645	110	13	2nd	2nd	ADJ
ajpf-645	110	14	antibodies	antibodie	VERB
ajpf-645	110	15	.	.	PUNCT
ajpf-645	111	1	(	(	PUNCT
ajpf-645	111	2	a	a	X
ajpf-645	111	3	)	)	PUNCT
ajpf-645	111	4	sds	sds	NOUN
ajpf-645	111	5	page	page	NOUN
ajpf-645	111	6	of	of	ADP
ajpf-645	111	7	total	total	ADJ
ajpf-645	111	8	lysates	lysate	NOUN
ajpf-645	111	9	.	.	PUNCT
ajpf-645	112	1	separated	separate	VERB
ajpf-645	112	2	bands	band	NOUN
ajpf-645	112	3	were	be	AUX
ajpf-645	112	4	visualized	visualize	VERB
ajpf-645	112	5	by	by	ADP
ajpf-645	112	6	coomassie	coomassie	ADJ
ajpf-645	112	7	staining	staining	NOUN
ajpf-645	112	8	.	.	PUNCT
ajpf-645	113	1	(	(	PUNCT
ajpf-645	113	2	b	b	X
ajpf-645	113	3	to	to	PART
ajpf-645	113	4	c	c	NOUN
ajpf-645	113	5	)	)	PUNCT
ajpf-645	113	6	immunoblot	immunoblot	NOUN
ajpf-645	113	7	of	of	ADP
ajpf-645	113	8	total	total	ADJ
ajpf-645	113	9	lysates	lysate	NOUN
ajpf-645	113	10	.	.	PUNCT
ajpf-645	114	1	his6	his6	PROPN
ajpf-645	114	2	tagged	tag	VERB
ajpf-645	114	3	ss	ss	PROPN
ajpf-645	114	4	was	be	AUX
ajpf-645	114	5	detected	detect	VERB
ajpf-645	114	6	by	by	ADP
ajpf-645	114	7	monoclonal	monoclonal	NOUN
ajpf-645	114	8	antibody	antibody	NOUN
ajpf-645	114	9	specific	specific	ADJ
ajpf-645	114	10	for	for	ADP
ajpf-645	114	11	his6	his6	NOUN
ajpf-645	114	12	tag	tag	NOUN
ajpf-645	114	13	(	(	PUNCT
ajpf-645	114	14	b	b	NOUN
ajpf-645	114	15	)	)	PUNCT
ajpf-645	114	16	,	,	PUNCT
ajpf-645	114	17	and	and	CCONJ
ajpf-645	114	18	expressed	express	VERB
ajpf-645	114	19	pspa	pspa	ADJ
ajpf-645	114	20	protein	protein	NOUN
ajpf-645	114	21	was	be	AUX
ajpf-645	114	22	detected	detect	VERB
ajpf-645	114	23	by	by	ADP
ajpf-645	114	24	monoclonal	monoclonal	NOUN
ajpf-645	114	25	antibody	antibody	NOUN
ajpf-645	114	26	specific	specific	ADJ
ajpf-645	114	27	for	for	ADP
ajpf-645	114	28	pspa	pspa	NOUN
ajpf-645	114	29	(	(	PUNCT
ajpf-645	114	30	c	c	NOUN
ajpf-645	114	31	)	)	PUNCT
ajpf-645	114	32	.	.	PUNCT
ajpf-645	115	1	(	(	PUNCT
ajpf-645	115	2	d	d	X
ajpf-645	115	3	)	)	PUNCT
ajpf-645	115	4	quantitative	quantitative	ADJ
ajpf-645	115	5	analysis	analysis	NOUN
ajpf-645	115	6	of	of	ADP
ajpf-645	115	7	pspa	pspa	ADJ
ajpf-645	115	8	protein	protein	NOUN
ajpf-645	115	9	.	.	PUNCT
ajpf-645	116	1	relative	relative	ADJ
ajpf-645	116	2	immuno	immuno	ADJ
ajpf-645	116	3	-	-	PUNCT
ajpf-645	116	4	reactive	reactive	ADJ
ajpf-645	116	5	intensities	intensity	NOUN
ajpf-645	116	6	were	be	AUX
ajpf-645	116	7	analyzed	analyze	VERB
ajpf-645	116	8	by	by	ADP
ajpf-645	116	9	imagej	imagej	ADJ
ajpf-645	116	10	program	program	NOUN
ajpf-645	116	11	.	.	PUNCT
ajpf-645	117	1	arrow	arrow	NOUN
ajpf-645	117	2	indicates	indicate	VERB
ajpf-645	117	3	the	the	DET
ajpf-645	117	4	pspa	pspa	ADJ
ajpf-645	117	5	protein	protein	NOUN
ajpf-645	117	6	.	.	PUNCT
ajpf-645	118	1	major	major	ADJ
ajpf-645	118	2	bands	band	NOUN
ajpf-645	118	3	between	between	ADP
ajpf-645	118	4	samples	sample	NOUN
ajpf-645	118	5	can	can	AUX
ajpf-645	118	6	be	be	AUX
ajpf-645	118	7	explained	explain	VERB
ajpf-645	118	8	by	by	ADP
ajpf-645	118	9	the	the	DET
ajpf-645	118	10	size	size	NOUN
ajpf-645	118	11	difference	difference	NOUN
ajpf-645	118	12	of	of	ADP
ajpf-645	118	13	signal	signal	NOUN
ajpf-645	118	14	peptide	peptide	NOUN
ajpf-645	118	15	which	which	PRON
ajpf-645	118	16	was	be	AUX
ajpf-645	118	17	fused	fuse	VERB
ajpf-645	118	18	to	to	ADP
ajpf-645	118	19	the	the	DET
ajpf-645	118	20	pspa	pspa	NOUN
ajpf-645	118	21	.	.	PUNCT
ajpf-645	119	1	the	the	DET
ajpf-645	119	2	37	37	NUM
ajpf-645	119	3	kda	kda	NOUN
ajpf-645	119	4	proteins	protein	NOUN
ajpf-645	119	5	were	be	AUX
ajpf-645	119	6	reacted	react	VERB
ajpf-645	119	7	with	with	ADP
ajpf-645	119	8	anti	anti	ADJ
ajpf-645	119	9	-	-	ADJ
ajpf-645	119	10	his6epitope	his6epitope	ADJ
ajpf-645	119	11	antibody	antibody	NOUN
ajpf-645	119	12	,	,	PUNCT
ajpf-645	119	13	which	which	PRON
ajpf-645	119	14	confirms	confirm	VERB
ajpf-645	119	15	that	that	SCONJ
ajpf-645	119	16	each	each	DET
ajpf-645	119	17	37	37	NUM
ajpf-645	119	18	kda	kda	NOUN
ajpf-645	119	19	protein	protein	NOUN
ajpf-645	119	20	contains	contain	VERB
ajpf-645	119	21	signal	signal	NOUN
ajpf-645	119	22	peptide	peptide	NOUN
ajpf-645	119	23	designed	design	VERB
ajpf-645	119	24	in	in	ADP
ajpf-645	119	25	this	this	DET
ajpf-645	119	26	study	study	NOUN
ajpf-645	119	27	.	.	PUNCT
ajpf-645	120	1	the	the	DET
ajpf-645	120	2	protein	protein	NOUN
ajpf-645	120	3	of	of	ADP
ajpf-645	120	4	s.	s.	PROPN
ajpf-645	120	5	typhimuium	typhimuium	PROPN
ajpf-645	120	6	8554	8554	PROPN
ajpf-645	120	7	harboring	harboring	NOUN
ajpf-645	120	8	pmmp67	pmmp67	PROPN
ajpf-645	120	9	exhibited	exhibit	VERB
ajpf-645	120	10	a	a	DET
ajpf-645	120	11	faint	faint	ADJ
ajpf-645	120	12	immune	immune	NOUN
ajpf-645	120	13	-	-	PUNCT
ajpf-645	120	14	reactive	reactive	NOUN
ajpf-645	120	15	band	band	NOUN
ajpf-645	120	16	,	,	PUNCT
ajpf-645	120	17	hypothesizing	hypothesize	VERB
ajpf-645	120	18	the	the	DET
ajpf-645	120	19	access	access	NOUN
ajpf-645	120	20	difficulty	difficulty	NOUN
ajpf-645	120	21	of	of	ADP
ajpf-645	120	22	h6	h6	NOUN
ajpf-645	120	23	-	-	PUNCT
ajpf-645	120	24	antibody	antibody	NOUN
ajpf-645	120	25	due	due	ADP
ajpf-645	120	26	to	to	ADP
ajpf-645	120	27	protein	protein	NOUN
ajpf-645	120	28	conformation	conformation	NOUN
ajpf-645	120	29	.	.	PUNCT
ajpf-645	121	1	as	as	SCONJ
ajpf-645	121	2	we	we	PRON
ajpf-645	121	3	expected	expect	VERB
ajpf-645	121	4	,	,	PUNCT
ajpf-645	121	5	there	there	PRON
ajpf-645	121	6	was	be	VERB
ajpf-645	121	7	no	no	DET
ajpf-645	121	8	his6	his6	NOUN
ajpf-645	121	9	-	-	PUNCT
ajpf-645	121	10	antibody	antibody	NOUN
ajpf-645	121	11	reaction	reaction	NOUN
ajpf-645	121	12	band	band	NOUN
ajpf-645	121	13	in	in	ADP
ajpf-645	121	14	sample	sample	NOUN
ajpf-645	121	15	of	of	ADP
ajpf-645	121	16	s.	s.	PROPN
ajpf-645	121	17	typhimuium	typhimuium	PROPN
ajpf-645	121	18	8554	8554	PROPN
ajpf-645	121	19	harboring	harboring	NOUN
ajpf-645	121	20	pya3494	pya3494	NOUN
ajpf-645	121	21	,	,	PUNCT
ajpf-645	121	22	which	which	PRON
ajpf-645	121	23	does	do	AUX
ajpf-645	121	24	not	not	PART
ajpf-645	121	25	carry	carry	VERB
ajpf-645	121	26	his6	his6	NOUN
ajpf-645	121	27	-	-	PUNCT
ajpf-645	121	28	tagged	tag	VERB
ajpf-645	121	29	signal	signal	NOUN
ajpf-645	121	30	peptide	peptide	NOUN
ajpf-645	121	31	.	.	PUNCT
ajpf-645	122	1	immunoblot	immunoblot	NOUN
ajpf-645	122	2	analyses	analysis	NOUN
ajpf-645	122	3	with	with	ADP
ajpf-645	122	4	anti	anti	ADJ
ajpf-645	122	5	-	-	ADJ
ajpf-645	122	6	pspa	pspa	ADJ
ajpf-645	122	7	antibody	antibody	NOUN
ajpf-645	122	8	demonstrated	demonstrate	VERB
ajpf-645	122	9	that	that	SCONJ
ajpf-645	122	10	the	the	DET
ajpf-645	122	11	37	37	NUM
ajpf-645	122	12	kda	kda	NOUN
ajpf-645	122	13	protein	protein	NOUN
ajpf-645	122	14	seen	see	VERB
ajpf-645	122	15	in	in	ADP
ajpf-645	122	16	all	all	DET
ajpf-645	122	17	samples	sample	NOUN
ajpf-645	122	18	figure	figure	VERB
ajpf-645	122	19	4	4	NUM
ajpf-645	122	20	.	.	PUNCT
ajpf-645	122	21	immunoblot	immunoblot	NOUN
ajpf-645	122	22	of	of	ADP
ajpf-645	122	23	secreted	secrete	VERB
ajpf-645	122	24	pspa	pspa	ADJ
ajpf-645	122	25	proteins	protein	NOUN
ajpf-645	122	26	.	.	PUNCT
ajpf-645	123	1	s.	s.	PROPN
ajpf-645	123	2	typhimurium	typhimurium	PRON
ajpf-645	123	3	8554	8554	PROPN
ajpf-645	123	4	harboring	harbor	VERB
ajpf-645	123	5	plasmids	plasmid	NOUN
ajpf-645	123	6	were	be	AUX
ajpf-645	123	7	cultured	cultured	ADJ
ajpf-645	123	8	in	in	ADP
ajpf-645	123	9	lb	lb	DET
ajpf-645	123	10	broth	broth	NOUN
ajpf-645	123	11	until	until	ADP
ajpf-645	123	12	exponential	exponential	ADJ
ajpf-645	123	13	phase	phase	NOUN
ajpf-645	123	14	(	(	PUNCT
ajpf-645	123	15	0.8	0.8	NUM
ajpf-645	123	16	at	at	ADP
ajpf-645	123	17	od600	od600	NOUN
ajpf-645	123	18	)	)	PUNCT
ajpf-645	123	19	.	.	PUNCT
ajpf-645	124	1	equal	equal	ADJ
ajpf-645	124	2	amount	amount	NOUN
ajpf-645	124	3	of	of	ADP
ajpf-645	124	4	cell	cell	NOUN
ajpf-645	124	5	-	-	PUNCT
ajpf-645	124	6	free	free	ADJ
ajpf-645	124	7	culture	culture	NOUN
ajpf-645	124	8	supernatant	supernatant	NOUN
ajpf-645	124	9	were	be	AUX
ajpf-645	124	10	subjected	subject	VERB
ajpf-645	124	11	to	to	ADP
ajpf-645	124	12	sds	sds	NOUN
ajpf-645	124	13	-	-	PUNCT
ajpf-645	124	14	page	page	NOUN
ajpf-645	124	15	and	and	CCONJ
ajpf-645	124	16	immunoblot	immunoblot	NOUN
ajpf-645	124	17	analyses	analysis	NOUN
ajpf-645	124	18	.	.	PUNCT
ajpf-645	125	1	(	(	PUNCT
ajpf-645	125	2	a	a	X
ajpf-645	125	3	)	)	PUNCT
ajpf-645	125	4	sds	sds	NOUN
ajpf-645	125	5	page	page	NOUN
ajpf-645	125	6	analysis	analysis	NOUN
ajpf-645	125	7	.	.	PUNCT
ajpf-645	126	1	separated	separate	VERB
ajpf-645	126	2	bands	band	NOUN
ajpf-645	126	3	were	be	AUX
ajpf-645	126	4	visualized	visualize	VERB
ajpf-645	126	5	by	by	ADP
ajpf-645	126	6	coomassie	coomassie	ADJ
ajpf-645	126	7	staining	staining	NOUN
ajpf-645	126	8	.	.	PUNCT
ajpf-645	127	1	(	(	PUNCT
ajpf-645	127	2	b	b	X
ajpf-645	127	3	)	)	PUNCT
ajpf-645	127	4	immunoblot	immunoblot	NOUN
ajpf-645	127	5	of	of	ADP
ajpf-645	127	6	secreted	secrete	VERB
ajpf-645	127	7	proteins	protein	NOUN
ajpf-645	127	8	.	.	PUNCT
ajpf-645	128	1	the	the	DET
ajpf-645	128	2	pspa	pspa	ADJ
ajpf-645	128	3	protein	protein	NOUN
ajpf-645	128	4	was	be	AUX
ajpf-645	128	5	detected	detect	VERB
ajpf-645	128	6	by	by	ADP
ajpf-645	128	7	monoclonal	monoclonal	NOUN
ajpf-645	128	8	antibody	antibody	NOUN
ajpf-645	128	9	specific	specific	ADJ
ajpf-645	128	10	for	for	ADP
ajpf-645	128	11	pspa	pspa	NOUN
ajpf-645	128	12	.	.	PUNCT
ajpf-645	129	1	(	(	PUNCT
ajpf-645	129	2	c	c	X
ajpf-645	129	3	)	)	PUNCT
ajpf-645	129	4	quantitative	quantitative	ADJ
ajpf-645	129	5	analysis	analysis	NOUN
ajpf-645	129	6	of	of	ADP
ajpf-645	129	7	pspa	pspa	ADJ
ajpf-645	129	8	protein	protein	NOUN
ajpf-645	129	9	.	.	PUNCT
ajpf-645	130	1	relative	relative	ADJ
ajpf-645	130	2	immuno	immuno	ADJ
ajpf-645	130	3	-	-	PUNCT
ajpf-645	130	4	reactive	reactive	ADJ
ajpf-645	130	5	intensities	intensity	NOUN
ajpf-645	130	6	were	be	AUX
ajpf-645	130	7	analyzed	analyze	VERB
ajpf-645	130	8	by	by	ADP
ajpf-645	130	9	imagej	imagej	ADJ
ajpf-645	130	10	program	program	NOUN
ajpf-645	130	11	.	.	PUNCT
ajpf-645	131	1	arrow	arrow	NOUN
ajpf-645	131	2	indicates	indicate	VERB
ajpf-645	131	3	the	the	DET
ajpf-645	131	4	pspa	pspa	ADJ
ajpf-645	131	5	protein	protein	NOUN
ajpf-645	131	6	.	.	PUNCT
ajpf-645	132	1	is	be	AUX
ajpf-645	132	2	the	the	DET
ajpf-645	132	3	pspa	pspa	ADJ
ajpf-645	132	4	protein	protein	NOUN
ajpf-645	132	5	.	.	PUNCT
ajpf-645	133	1	the	the	DET
ajpf-645	133	2	highest	high	ADJ
ajpf-645	133	3	amount	amount	NOUN
ajpf-645	133	4	of	of	ADP
ajpf-645	133	5	cell	cell	NOUN
ajpf-645	133	6	associated	associate	VERB
ajpf-645	133	7	pspa	pspa	NOUN
ajpf-645	133	8	protein	protein	NOUN
ajpf-645	133	9	was	be	AUX
ajpf-645	133	10	detected	detect	VERB
ajpf-645	133	11	in	in	ADP
ajpf-645	133	12	salmonella	salmonella	NOUN
ajpf-645	133	13	containing	contain	VERB
ajpf-645	133	14	pya3494	pya3494	NOUN
ajpf-645	133	15	,	,	PUNCT
ajpf-645	133	16	which	which	PRON
ajpf-645	133	17	was	be	AUX
ajpf-645	133	18	almost	almost	ADV
ajpf-645	133	19	2	2	NUM
ajpf-645	133	20	fold	fold	ADJ
ajpf-645	133	21	higher	high	ADJ
ajpf-645	133	22	levels	level	NOUN
ajpf-645	133	23	than	than	ADP
ajpf-645	133	24	that	that	PRON
ajpf-645	133	25	in	in	ADP
ajpf-645	133	26	salmonella	salmonella	NOUN
ajpf-645	133	27	containing	contain	VERB
ajpf-645	133	28	pmmp70	pmmp70	NOUN
ajpf-645	133	29	(	(	PUNCT
ajpf-645	133	30	carrying	carry	VERB
ajpf-645	133	31	ompw	ompw	NOUN
ajpf-645	133	32	ss	ss	PROPN
ajpf-645	133	33	)	)	PUNCT
ajpf-645	133	34	.	.	PUNCT
ajpf-645	134	1	the	the	DET
ajpf-645	134	2	salmonella	salmonella	NOUN
ajpf-645	134	3	containing	contain	VERB
ajpf-645	134	4	pmmp67	pmmp67	NOUN
ajpf-645	134	5	or	or	CCONJ
ajpf-645	134	6	pmmp66	pmmp66	PROPN
ajpf-645	134	7	expressed	express	VERB
ajpf-645	134	8	pspa	pspa	NOUN
ajpf-645	134	9	1.5	1.5	NUM
ajpf-645	134	10	or	or	CCONJ
ajpf-645	134	11	1.3	1.3	NUM
ajpf-645	134	12	folds	fold	NOUN
ajpf-645	134	13	higher	high	ADJ
ajpf-645	134	14	than	than	ADP
ajpf-645	134	15	salmonella	salmonella	NOUN
ajpf-645	134	16	containing	contain	VERB
ajpf-645	134	17	pmmp68	pmmp68	NOUN
ajpf-645	134	18	.	.	PUNCT
ajpf-645	135	1	an	an	DET
ajpf-645	135	2	advantage	advantage	NOUN
ajpf-645	135	3	of	of	ADP
ajpf-645	135	4	the	the	DET
ajpf-645	135	5	system	system	NOUN
ajpf-645	135	6	developed	develop	VERB
ajpf-645	135	7	in	in	ADP
ajpf-645	135	8	this	this	DET
ajpf-645	135	9	study	study	NOUN
ajpf-645	135	10	is	be	AUX
ajpf-645	135	11	the	the	DET
ajpf-645	135	12	detection	detection	NOUN
ajpf-645	135	13	of	of	ADP
ajpf-645	135	14	the	the	DET
ajpf-645	135	15	expressed	express	VERB
ajpf-645	135	16	protein	protein	NOUN
ajpf-645	135	17	with	with	ADP
ajpf-645	135	18	commercially	commercially	ADV
ajpf-645	135	19	available	available	ADJ
ajpf-645	135	20	his6	his6	NOUN
ajpf-645	135	21	-	-	PUNCT
ajpf-645	135	22	epitope	epitope	NOUN
ajpf-645	135	23	antibody	antibody	NOUN
ajpf-645	135	24	,	,	PUNCT
ajpf-645	135	25	without	without	ADP
ajpf-645	135	26	specific	specific	ADJ
ajpf-645	135	27	antibody	antibody	NOUN
ajpf-645	135	28	for	for	ADP
ajpf-645	135	29	the	the	DET
ajpf-645	135	30	expressed	express	VERB
ajpf-645	135	31	protein	protein	NOUN
ajpf-645	135	32	.	.	PUNCT
ajpf-645	136	1	identification	identification	NOUN
ajpf-645	136	2	of	of	ADP
ajpf-645	136	3	secretion	secretion	NOUN
ajpf-645	136	4	ability	ability	NOUN
ajpf-645	136	5	according	accord	VERB
ajpf-645	136	6	to	to	PART
ajpf-645	136	7	signal	signal	VERB
ajpf-645	136	8	sequences	sequence	NOUN
ajpf-645	136	9	the	the	DET
ajpf-645	136	10	protein	protein	NOUN
ajpf-645	136	11	expression	expression	NOUN
ajpf-645	136	12	system	system	NOUN
ajpf-645	136	13	developed	develop	VERB
ajpf-645	136	14	in	in	ADP
ajpf-645	136	15	this	this	DET
ajpf-645	136	16	study	study	NOUN
ajpf-645	136	17	is	be	AUX
ajpf-645	136	18	designed	design	VERB
ajpf-645	136	19	for	for	ADP
ajpf-645	136	20	the	the	DET
ajpf-645	136	21	secretion	secretion	NOUN
ajpf-645	136	22	of	of	ADP
ajpf-645	136	23	the	the	DET
ajpf-645	136	24	pspa	pspa	ADJ
ajpf-645	136	25	protein	protein	NOUN
ajpf-645	136	26	.	.	PUNCT
ajpf-645	137	1	each	each	DET
ajpf-645	137	2	signal	signal	NOUN
ajpf-645	137	3	peptide	peptide	NOUN
ajpf-645	137	4	fused	fuse	VERB
ajpf-645	137	5	to	to	ADP
ajpf-645	137	6	pspa	pspa	NOUN
ajpf-645	137	7	may	may	AUX
ajpf-645	137	8	lead	lead	VERB
ajpf-645	137	9	to	to	ADP
ajpf-645	137	10	the	the	DET
ajpf-645	137	11	translocation	translocation	NOUN
ajpf-645	137	12	of	of	ADP
ajpf-645	137	13	the	the	DET
ajpf-645	137	14	cytoplasmic	cytoplasmic	ADJ
ajpf-645	137	15	pspa	pspa	NOUN
ajpf-645	137	16	to	to	ADP
ajpf-645	137	17	the	the	DET
ajpf-645	137	18	cell	cell	NOUN
ajpf-645	137	19	envelop	envelop	VERB
ajpf-645	137	20	or	or	CCONJ
ajpf-645	137	21	to	to	ADP
ajpf-645	137	22	the	the	DET
ajpf-645	137	23	outside	outside	NOUN
ajpf-645	137	24	of	of	ADP
ajpf-645	137	25	the	the	DET
ajpf-645	137	26	cell	cell	NOUN
ajpf-645	137	27	.	.	PUNCT
ajpf-645	138	1	to	to	PART
ajpf-645	138	2	examine	examine	VERB
ajpf-645	138	3	the	the	DET
ajpf-645	138	4	level	level	NOUN
ajpf-645	138	5	of	of	ADP
ajpf-645	138	6	protein	protein	NOUN
ajpf-645	138	7	secretion	secretion	NOUN
ajpf-645	138	8	,	,	PUNCT
ajpf-645	138	9	the	the	DET
ajpf-645	138	10	s.	s.	PROPN
ajpf-645	138	11	typhimuium	typhimuium	NOUN
ajpf-645	138	12	8554	8554	PROPN
ajpf-645	138	13	carrying	carry	VERB
ajpf-645	138	14	pya3494	pya3494	NOUN
ajpf-645	138	15	,	,	PUNCT
ajpf-645	138	16	pmmp66	pmmp66	NOUN
ajpf-645	138	17	,	,	PUNCT
ajpf-645	138	18	pmmp67	pmmp67	PROPN
ajpf-645	138	19	,	,	PUNCT
ajpf-645	138	20	pmmp68	pmmp68	ADJ
ajpf-645	138	21	or	or	CCONJ
ajpf-645	138	22	pmmp70	pmmp70	NOUN
ajpf-645	138	23	were	be	AUX
ajpf-645	138	24	cultured	cultured	ADJ
ajpf-645	138	25	to	to	ADP
ajpf-645	138	26	od600	od600	ADP
ajpf-645	138	27	0.8	0.8	NUM
ajpf-645	138	28	in	in	ADP
ajpf-645	138	29	lb	lb	DET
ajpf-645	138	30	broth	broth	NOUN
ajpf-645	138	31	at	at	ADP
ajpf-645	138	32	37	37	NUM
ajpf-645	138	33	°	°	NOUN
ajpf-645	138	34	c	c	NOUN
ajpf-645	138	35	.	.	PUNCT
ajpf-645	139	1	cell	cell	NOUN
ajpf-645	139	2	-	-	PUNCT
ajpf-645	139	3	free	free	ADJ
ajpf-645	139	4	supernatant	supernatant	NOUN
ajpf-645	139	5	of	of	ADP
ajpf-645	139	6	each	each	DET
ajpf-645	139	7	culture	culture	NOUN
ajpf-645	139	8	was	be	AUX
ajpf-645	139	9	examined	examine	VERB
ajpf-645	139	10	for	for	ADP
ajpf-645	139	11	the	the	DET
ajpf-645	139	12	secreted	secrete	VERB
ajpf-645	139	13	protein	protein	NOUN
ajpf-645	139	14	fractions	fraction	NOUN
ajpf-645	139	15	.	.	PUNCT
ajpf-645	140	1	to	to	PART
ajpf-645	140	2	eliminate	eliminate	VERB
ajpf-645	140	3	the	the	DET
ajpf-645	140	4	presence	presence	NOUN
ajpf-645	140	5	of	of	ADP
ajpf-645	140	6	salmonella	salmonella	NOUN
ajpf-645	140	7	cells	cell	NOUN
ajpf-645	140	8	entirely	entirely	ADV
ajpf-645	140	9	,	,	PUNCT
ajpf-645	140	10	culture	culture	NOUN
ajpf-645	140	11	supernatant	supernatant	NOUN
ajpf-645	140	12	after	after	SCONJ
ajpf-645	140	13	centrifugation	centrifugation	NOUN
ajpf-645	140	14	was	be	AUX
ajpf-645	140	15	filtered	filter	VERB
ajpf-645	140	16	through	through	ADP
ajpf-645	140	17	passing	pass	VERB
ajpf-645	140	18	0.2	0.2	NUM
ajpf-645	140	19	µm	µm	ADP
ajpf-645	140	20	filter	filter	NOUN
ajpf-645	140	21	.	.	PUNCT
ajpf-645	141	1	the	the	DET
ajpf-645	141	2	proteins	protein	NOUN
ajpf-645	141	3	in	in	ADP
ajpf-645	141	4	1	1	NUM
ajpf-645	141	5	ml	ml	NOUN
ajpf-645	141	6	of	of	ADP
ajpf-645	141	7	supernatant	supernatant	NOUN
ajpf-645	141	8	were	be	AUX
ajpf-645	141	9	precipitated	precipitate	VERB
ajpf-645	141	10	using	use	VERB
ajpf-645	141	11	10	10	NUM
ajpf-645	141	12	%	%	NOUN
ajpf-645	141	13	tca	tca	PROPN
ajpf-645	141	14	as	as	SCONJ
ajpf-645	141	15	described	describe	VERB
ajpf-645	141	16	earlier	early	ADV
ajpf-645	141	17	.	.	PUNCT
ajpf-645	142	1	the	the	DET
ajpf-645	142	2	precipitated	precipitate	VERB
ajpf-645	142	3	pellets	pellet	NOUN
ajpf-645	142	4	after	after	ADP
ajpf-645	142	5	washing	wash	VERB
ajpf-645	142	6	twice	twice	ADV
ajpf-645	142	7	were	be	AUX
ajpf-645	142	8	resuspended	resuspend	VERB
ajpf-645	142	9	in	in	ADP
ajpf-645	142	10	60	60	NUM
ajpf-645	142	11	l	l	PROPN
ajpf-645	142	12	of	of	ADP
ajpf-645	142	13	sds	sds	PROPN
ajpf-645	142	14	loading	loading	NOUN
ajpf-645	142	15	buffer	buffer	NOUN
ajpf-645	142	16	.	.	PUNCT
ajpf-645	143	1	8	8	NUM
ajpf-645	143	2	g	g	PROPN
ajpf-645	143	3	protein	protein	NOUN
ajpf-645	143	4	of	of	ADP
ajpf-645	143	5	each	each	DET
ajpf-645	143	6	sample	sample	NOUN
ajpf-645	143	7	was	be	AUX
ajpf-645	143	8	subjected	subject	VERB
ajpf-645	143	9	to	to	ADP
ajpf-645	143	10	sds	sds	NOUN
ajpf-645	143	11	-	-	PUNCT
ajpf-645	143	12	page	page	NOUN
ajpf-645	143	13	.	.	PUNCT
ajpf-645	144	1	parts	part	NOUN
ajpf-645	144	2	of	of	ADP
ajpf-645	144	3	samples	sample	NOUN
ajpf-645	144	4	were	be	AUX
ajpf-645	144	5	employed	employ	VERB
ajpf-645	144	6	for	for	ADP
ajpf-645	144	7	immunoblot	immunoblot	NOUN
ajpf-645	144	8	analysis	analysis	NOUN
ajpf-645	144	9	after	after	ADP
ajpf-645	144	10	sds	sds	NOUN
ajpf-645	144	11	-	-	PUNCT
ajpf-645	144	12	page	page	NOUN
ajpf-645	144	13	.	.	PUNCT
ajpf-645	145	1	all	all	DET
ajpf-645	145	2	samples	sample	NOUN
ajpf-645	145	3	exhibited	exhibit	VERB
ajpf-645	145	4	a	a	DET
ajpf-645	145	5	major	major	ADJ
ajpf-645	145	6	protein	protein	NOUN
ajpf-645	145	7	bands	band	NOUN
ajpf-645	145	8	with	with	ADP
ajpf-645	145	9	approximately	approximately	ADV
ajpf-645	145	10	37	37	NUM
ajpf-645	145	11	kda	kda	NOUN
ajpf-645	145	12	size	size	NOUN
ajpf-645	145	13	likely	likely	ADV
ajpf-645	145	14	as	as	SCONJ
ajpf-645	145	15	seen	see	VERB
ajpf-645	145	16	in	in	ADP
ajpf-645	145	17	total	total	ADJ
ajpf-645	145	18	lysates	lysate	NOUN
ajpf-645	145	19	(	(	PUNCT
ajpf-645	145	20	figure	figure	NOUN
ajpf-645	145	21	4	4	NUM
ajpf-645	145	22	)	)	PUNCT
ajpf-645	145	23	.	.	PUNCT
ajpf-645	146	1	the	the	DET
ajpf-645	146	2	37	37	NUM
ajpf-645	146	3	kda	kda	NOUN
ajpf-645	146	4	proteins	protein	NOUN
ajpf-645	146	5	were	be	AUX
ajpf-645	146	6	reacted	react	VERB
ajpf-645	146	7	with	with	ADP
ajpf-645	146	8	both	both	CCONJ
ajpf-645	146	9	anti	anti	ADJ
ajpf-645	146	10	-	-	ADJ
ajpf-645	146	11	his6	his6	ADJ
ajpf-645	146	12	epitope	epitope	NOUN
ajpf-645	146	13	antibody	antibody	NOUN
ajpf-645	146	14	and	and	CCONJ
ajpf-645	146	15	anti	anti	ADJ
ajpf-645	146	16	-	-	ADJ
ajpf-645	146	17	pspa	pspa	ADJ
ajpf-645	146	18	antibody	antibody	NOUN
ajpf-645	146	19	,	,	PUNCT
ajpf-645	146	20	demonstrating	demonstrate	VERB
ajpf-645	146	21	pspa	pspa	ADJ
ajpf-645	146	22	protein	protein	NOUN
ajpf-645	146	23	of	of	ADP
ajpf-645	146	24	the	the	DET
ajpf-645	146	25	37	37	NUM
ajpf-645	146	26	kda	kda	NOUN
ajpf-645	146	27	protein	protein	NOUN
ajpf-645	146	28	.	.	PUNCT
ajpf-645	147	1	interestingly	interestingly	ADV
ajpf-645	147	2	,	,	PUNCT
ajpf-645	147	3	a	a	DET
ajpf-645	147	4	thick	thick	ADJ
ajpf-645	147	5	37	37	NUM
ajpf-645	147	6	kda	kda	NOUN
ajpf-645	147	7	protein	protein	NOUN
ajpf-645	147	8	band	band	NOUN
ajpf-645	147	9	was	be	AUX
ajpf-645	147	10	observed	observe	VERB
ajpf-645	147	11	in	in	ADP
ajpf-645	147	12	the	the	DET
ajpf-645	147	13	secreted	secrete	VERB
ajpf-645	147	14	fraction	fraction	NOUN
ajpf-645	147	15	of	of	ADP
ajpf-645	147	16	s.	s.	PROPN
ajpf-645	147	17	typhimuium	typhimuium	NOUN
ajpf-645	147	18	8554	8554	PROPN
ajpf-645	147	19	carrying	carry	VERB
ajpf-645	147	20	pmmp67	pmmp67	NOUN
ajpf-645	147	21	.	.	PUNCT
ajpf-645	148	1	densitometer	densitometer	VERB
ajpf-645	148	2	analysis	analysis	NOUN
ajpf-645	148	3	of	of	ADP
ajpf-645	148	4	the	the	DET
ajpf-645	148	5	secreted	secrete	VERB
ajpf-645	148	6	pspa	pspa	NOUN
ajpf-645	148	7	in	in	ADP
ajpf-645	148	8	each	each	DET
ajpf-645	148	9	sample	sample	NOUN
ajpf-645	148	10	shows	show	VERB
ajpf-645	148	11	that	that	SCONJ
ajpf-645	148	12	the	the	DET
ajpf-645	148	13	secreted	secrete	VERB
ajpf-645	148	14	pspa	pspa	NOUN
ajpf-645	148	15	in	in	ADP
ajpf-645	148	16	salmonella	salmonella	NOUN
ajpf-645	148	17	carrying	carry	VERB
ajpf-645	148	18	pmm67	pmm67	PROPN
ajpf-645	148	19	was	be	AUX
ajpf-645	148	20	1.8	1.8	NUM
ajpf-645	148	21	fold	fold	NOUN
ajpf-645	148	22	amount	amount	NOUN
ajpf-645	148	23	higher	high	ADJ
ajpf-645	148	24	than	than	ADP
ajpf-645	148	25	that	that	PRON
ajpf-645	148	26	in	in	ADP
ajpf-645	148	27	salmonella	salmonella	NOUN
ajpf-645	148	28	carrying	carry	VERB
ajpf-645	148	29	pmm70	pmm70	PROPN
ajpf-645	148	30	which	which	PRON
ajpf-645	148	31	secrets	secret	VERB
ajpf-645	148	32	the	the	DET
ajpf-645	148	33	protein	protein	NOUN
ajpf-645	148	34	second	second	ADJ
ajpf-645	148	35	level	level	NOUN
ajpf-645	148	36	.	.	PUNCT
ajpf-645	149	1	the	the	DET
ajpf-645	149	2	other	other	ADJ
ajpf-645	149	3	three	three	NUM
ajpf-645	149	4	samples	sample	NOUN
ajpf-645	149	5	,	,	PUNCT
ajpf-645	149	6	salmonella	salmonella	NOUN
ajpf-645	149	7	carrying	carry	VERB
ajpf-645	149	8	pya3494	pya3494	NOUN
ajpf-645	149	9	,	,	PUNCT
ajpf-645	149	10	pmm66	pmm66	VERB
ajpf-645	149	11	and	and	CCONJ
ajpf-645	149	12	pmm68	pmm68	NOUN
ajpf-645	149	13	secrets	secret	NOUN
ajpf-645	149	14	the	the	DET
ajpf-645	149	15	pspa	pspa	NOUN
ajpf-645	149	16	with	with	ADP
ajpf-645	149	17	similar	similar	ADJ
ajpf-645	149	18	levels	level	NOUN
ajpf-645	149	19	.	.	PUNCT
ajpf-645	150	1	although	although	SCONJ
ajpf-645	150	2	the	the	DET
ajpf-645	150	3	pspa	pspa	ADJ
ajpf-645	150	4	level	level	NOUN
ajpf-645	150	5	in	in	ADP
ajpf-645	150	6	total	total	ADJ
ajpf-645	150	7	cell	cell	NOUN
ajpf-645	150	8	lysate	lysate	NOUN
ajpf-645	150	9	was	be	AUX
ajpf-645	150	10	similar	similar	ADJ
ajpf-645	150	11	for	for	ADP
ajpf-645	150	12	pya3494	pya3494	NOUN
ajpf-645	150	13	,	,	PUNCT
ajpf-645	150	14	pmmp67	pmmp67	NOUN
ajpf-645	150	15	and	and	CCONJ
ajpf-645	150	16	pmmp70	pmmp70	NOUN
ajpf-645	150	17	(	(	PUNCT
ajpf-645	150	18	figure	figure	NOUN
ajpf-645	150	19	3	3	NUM
ajpf-645	150	20	)	)	PUNCT
ajpf-645	150	21	,	,	PUNCT
ajpf-645	150	22	the	the	DET
ajpf-645	150	23	highest	high	ADJ
ajpf-645	150	24	amount	amount	NOUN
ajpf-645	150	25	of	of	ADP
ajpf-645	150	26	secreted	secrete	VERB
ajpf-645	150	27	pspa	pspa	NOUN
ajpf-645	150	28	was	be	AUX
ajpf-645	150	29	detected	detect	VERB
ajpf-645	150	30	in	in	ADP
ajpf-645	150	31	the	the	DET
ajpf-645	150	32	supernatant	supernatant	NOUN
ajpf-645	150	33	of	of	ADP
ajpf-645	150	34	salmonella	salmonella	NOUN
ajpf-645	150	35	carrying	carry	VERB
ajpf-645	150	36	pmm67	pmm67	NOUN
ajpf-645	150	37	.	.	PUNCT
ajpf-645	151	1	this	this	DET
ajpf-645	151	2	discrepancy	discrepancy	NOUN
ajpf-645	151	3	is	be	AUX
ajpf-645	151	4	not	not	PART
ajpf-645	151	5	solely	solely	ADV
ajpf-645	151	6	because	because	SCONJ
ajpf-645	151	7	of	of	ADP
ajpf-645	151	8	overexpression	overexpression	NOUN
ajpf-645	151	9	of	of	ADP
ajpf-645	151	10	the	the	DET
ajpf-645	151	11	protein	protein	NOUN
ajpf-645	151	12	,	,	PUNCT
ajpf-645	151	13	but	but	CCONJ
ajpf-645	151	14	it	it	PRON
ajpf-645	151	15	might	might	AUX
ajpf-645	151	16	be	be	AUX
ajpf-645	151	17	due	due	ADJ
ajpf-645	151	18	to	to	ADP
ajpf-645	151	19	the	the	DET
ajpf-645	151	20	agf	agf	PROPN
ajpf-645	151	21	ss	ss	PROPN
ajpf-645	151	22	.	.	PUNCT
ajpf-645	152	1	thus	thus	ADV
ajpf-645	152	2	,	,	PUNCT
ajpf-645	152	3	we	we	PRON
ajpf-645	152	4	speculate	speculate	VERB
ajpf-645	152	5	that	that	SCONJ
ajpf-645	152	6	the	the	DET
ajpf-645	152	7	agfa	agfa	PROPN
ajpf-645	152	8	signal	signal	PROPN
ajpf-645	152	9	peptide	peptide	PROPN
ajpf-645	152	10	mediates	mediates	PROPN
ajpf-645	152	11	pspa	pspa	PROPN
ajpf-645	152	12	secretion	secretion	NOUN
ajpf-645	152	13	more	more	ADV
ajpf-645	152	14	efficiently	efficiently	ADV
ajpf-645	152	15	than	than	ADP
ajpf-645	152	16	any	any	DET
ajpf-645	152	17	other	other	ADJ
ajpf-645	152	18	signal	signal	NOUN
ajpf-645	152	19	peptides	peptide	NOUN
ajpf-645	152	20	used	use	VERB
ajpf-645	152	21	in	in	ADP
ajpf-645	152	22	this	this	DET
ajpf-645	152	23	study	study	NOUN
ajpf-645	152	24	.	.	PUNCT
ajpf-645	153	1	taken	take	VERB
ajpf-645	153	2	all	all	ADV
ajpf-645	153	3	together	together	ADV
ajpf-645	153	4	,	,	PUNCT
ajpf-645	153	5	since	since	SCONJ
ajpf-645	153	6	signal	signal	ADJ
ajpf-645	153	7	sequences	sequence	NOUN
ajpf-645	153	8	play	play	VERB
ajpf-645	153	9	roles	role	NOUN
ajpf-645	153	10	as	as	ADP
ajpf-645	153	11	a	a	DET
ajpf-645	153	12	leader	leader	NOUN
ajpf-645	153	13	sequence	sequence	NOUN
ajpf-645	153	14	and	and	CCONJ
ajpf-645	153	15	a	a	DET
ajpf-645	153	16	signal	signal	ADJ
ajpf-645	153	17	sequence	sequence	NOUN
ajpf-645	153	18	,	,	PUNCT
ajpf-645	153	19	their	their	PRON
ajpf-645	153	20	existences	existence	NOUN
ajpf-645	153	21	raise	raise	VERB
ajpf-645	153	22	individual	individual	ADJ
ajpf-645	153	23	expression	expression	NOUN
ajpf-645	153	24	and	and	CCONJ
ajpf-645	153	25	secretion	secretion	NOUN
ajpf-645	153	26	of	of	ADP
ajpf-645	153	27	protein	protein	NOUN
ajpf-645	153	28	.	.	PUNCT
ajpf-645	154	1	the	the	DET
ajpf-645	154	2	efficient	efficient	ADJ
ajpf-645	154	3	protein	protein	NOUN
ajpf-645	154	4	secretion	secretion	NOUN
ajpf-645	154	5	mediated	mediate	VERB
ajpf-645	154	6	by	by	ADP
ajpf-645	154	7	agfa	agfa	PROPN
ajpf-645	154	8	signal	signal	PROPN
ajpf-645	154	9	sequence	sequence	NOUN
ajpf-645	154	10	can	can	AUX
ajpf-645	154	11	extend	extend	VERB
ajpf-645	154	12	its	its	PRON
ajpf-645	154	13	utility	utility	NOUN
ajpf-645	154	14	in	in	ADP
ajpf-645	154	15	various	various	ADJ
ajpf-645	154	16	fields	field	NOUN
ajpf-645	154	17	.	.	PUNCT
ajpf-645	155	1	especially	especially	ADV
ajpf-645	155	2	,	,	PUNCT
ajpf-645	155	3	it	it	PRON
ajpf-645	155	4	can	can	AUX
ajpf-645	155	5	be	be	AUX
ajpf-645	155	6	applicable	applicable	ADJ
ajpf-645	155	7	in	in	ADP
ajpf-645	155	8	the	the	DET
ajpf-645	155	9	antigen	antigen	ADJ
ajpf-645	155	10	delivery	delivery	NOUN
ajpf-645	155	11	of	of	ADP
ajpf-645	155	12	recombinant	recombinant	ADJ
ajpf-645	155	13	attenuated	attenuate	VERB
ajpf-645	155	14	salmonella	salmonella	NOUN
ajpf-645	155	15	vaccines	vaccine	NOUN
ajpf-645	155	16	(	(	PUNCT
ajpf-645	155	17	kim	kim	PROPN
ajpf-645	155	18	et	et	PROPN
ajpf-645	155	19	al	al	PROPN
ajpf-645	155	20	.	.	PROPN
ajpf-645	155	21	,	,	PUNCT
ajpf-645	155	22	2009	2009	NUM
ajpf-645	155	23	)	)	PUNCT
ajpf-645	155	24	.	.	PUNCT
ajpf-645	156	1	a	a	DET
ajpf-645	156	2	previous	previous	ADJ
ajpf-645	156	3	study	study	NOUN
ajpf-645	156	4	suggested	suggest	VERB
ajpf-645	156	5	that	that	SCONJ
ajpf-645	156	6	salmonella	salmonella	NOUN
ajpf-645	156	7	vaccine	vaccine	NOUN
ajpf-645	156	8	secreting	secrete	VERB
ajpf-645	156	9	foreign	foreign	ADJ
ajpf-645	156	10	antigen	antigen	NOUN
ajpf-645	156	11	induces	induce	VERB
ajpf-645	156	12	better	well	ADJ
ajpf-645	156	13	immune	immune	ADJ
ajpf-645	156	14	responses	response	NOUN
ajpf-645	156	15	than	than	ADP
ajpf-645	156	16	the	the	DET
ajpf-645	156	17	vaccine	vaccine	NOUN
ajpf-645	156	18	localizing	localize	VERB
ajpf-645	156	19	the	the	DET
ajpf-645	156	20	antigen	antigen	NOUN
ajpf-645	156	21	in	in	ADP
ajpf-645	156	22	cytoplasm	cytoplasm	NOUN
ajpf-645	156	23	(	(	PUNCT
ajpf-645	156	24	kang	kang	PROPN
ajpf-645	156	25	et	et	PROPN
ajpf-645	156	26	al	al	PROPN
ajpf-645	156	27	.	.	PROPN
ajpf-645	156	28	,	,	PUNCT
ajpf-645	156	29	2002	2002	NUM
ajpf-645	156	30	)	)	PUNCT
ajpf-645	156	31	.	.	PUNCT
ajpf-645	157	1	therefore	therefore	ADV
ajpf-645	157	2	,	,	PUNCT
ajpf-645	157	3	more	more	ADJ
ajpf-645	157	4	secretion	secretion	NOUN
ajpf-645	157	5	of	of	ADP
ajpf-645	157	6	the	the	DET
ajpf-645	157	7	foreign	foreign	ADJ
ajpf-645	157	8	antigen	antigen	NOUN
ajpf-645	157	9	may	may	AUX
ajpf-645	157	10	induce	induce	VERB
ajpf-645	157	11	more	more	ADJ
ajpf-645	157	12	immune	immune	ADJ
ajpf-645	157	13	responses	response	NOUN
ajpf-645	157	14	in	in	ADP
ajpf-645	157	15	salmonella	salmonella	NOUN
ajpf-645	157	16	antigen	antigen	ADJ
ajpf-645	157	17	delivery	delivery	NOUN
ajpf-645	157	18	system	system	NOUN
ajpf-645	157	19	.	.	PUNCT
ajpf-645	158	1	acknowledgements	acknowledgement	NOUN
ajpf-645	158	2	this	this	DET
ajpf-645	158	3	work	work	NOUN
ajpf-645	158	4	was	be	AUX
ajpf-645	158	5	supported	support	VERB
ajpf-645	158	6	by	by	ADP
ajpf-645	158	7	priority	priority	NOUN
ajpf-645	158	8	research	research	NOUN
ajpf-645	158	9	centers	center	NOUN
ajpf-645	158	10	program	program	NOUN
ajpf-645	158	11	(	(	PUNCT
ajpf-645	158	12	2009	2009	NUM
ajpf-645	158	13	-	-	SYM
ajpf-645	158	14	0093813	0093813	NUM
ajpf-645	158	15	)	)	PUNCT
ajpf-645	158	16	through	through	ADP
ajpf-645	158	17	the	the	DET
ajpf-645	158	18	national	national	PROPN
ajpf-645	158	19	research	research	PROPN
ajpf-645	158	20	foundation	foundation	PROPN
ajpf-645	158	21	of	of	ADP
ajpf-645	158	22	korea	korea	PROPN
ajpf-645	158	23	(	(	PUNCT
ajpf-645	158	24	nrf	nrf	NOUN
ajpf-645	158	25	)	)	PUNCT
ajpf-645	158	26	funded	fund	VERB
ajpf-645	158	27	by	by	ADP
ajpf-645	158	28	the	the	DET
ajpf-645	158	29	ministry	ministry	PROPN
ajpf-645	158	30	of	of	ADP
ajpf-645	158	31	education	education	PROPN
ajpf-645	158	32	,	,	PUNCT
ajpf-645	158	33	science	science	NOUN
ajpf-645	158	34	and	and	CCONJ
ajpf-645	158	35	technology	technology	NOUN
ajpf-645	158	36	of	of	ADP
ajpf-645	158	37	korea	korea	PROPN
ajpf-645	158	38	and	and	CCONJ
ajpf-645	158	39	by	by	ADP
ajpf-645	158	40	technology	technology	NOUN
ajpf-645	158	41	development	development	NOUN
ajpf-645	158	42	program	program	NOUN
ajpf-645	158	43	for	for	ADP
ajpf-645	158	44	(	(	PUNCT
ajpf-645	158	45	agriculture	agriculture	NOUN
ajpf-645	158	46	and	and	CCONJ
ajpf-645	158	47	forestry	forestry	NOUN
ajpf-645	158	48	or	or	CCONJ
ajpf-645	158	49	food	food	NOUN
ajpf-645	158	50	or	or	CCONJ
ajpf-645	158	51	fisheries	fishery	NOUN
ajpf-645	158	52	)	)	PUNCT
ajpf-645	158	53	,	,	PUNCT
ajpf-645	158	54	ministry	ministry	NOUN
ajpf-645	158	55	for	for	ADP
ajpf-645	158	56	food	food	NOUN
ajpf-645	158	57	,	,	PUNCT
ajpf-645	158	58	agriculture	agriculture	NOUN
ajpf-645	158	59	,	,	PUNCT
ajpf-645	158	60	forestry	forestry	NOUN
ajpf-645	158	61	and	and	CCONJ
ajpf-645	158	62	fisheries	fishery	NOUN
ajpf-645	158	63	,	,	PUNCT
ajpf-645	158	64	republic	republic	NOUN
ajpf-645	158	65	of	of	ADP
ajpf-645	158	66	korea	korea	PROPN
ajpf-645	158	67	.	.	PUNCT
ajpf-645	159	1	abbreviations	abbreviation	NOUN
ajpf-645	159	2	:	:	PUNCT
ajpf-645	159	3	ss	ss	NOUN
ajpf-645	159	4	,	,	PUNCT
ajpf-645	159	5	signal	signal	PROPN
ajpf-645	159	6	sequencel	sequencel	ADV
ajpf-645	159	7	;	;	PUNCT
ajpf-645	159	8	bla	bla	NOUN
ajpf-645	159	9	,	,	PUNCT
ajpf-645	159	10	-lactamase	-lactamase	PROPN
ajpf-645	159	11	;	;	PUNCT
ajpf-645	159	12	agfa	agfa	PROPN
ajpf-645	159	13	,	,	PUNCT
ajpf-645	159	14	thin	thin	PROPN
ajpf-645	159	15	aggregative	aggregative	ADJ
ajpf-645	159	16	fimbriae	fimbriae	NOUN
ajpf-645	159	17	a	a	PRON
ajpf-645	159	18	;	;	PUNCT
ajpf-645	159	19	stfa	stfa	NOUN
ajpf-645	159	20	,	,	PUNCT
ajpf-645	159	21	salmonella	salmonella	NOUN
ajpf-645	159	22	typhimurium	typhimurium	DET
ajpf-645	159	23	fimbriae	fimbriae	NOUN
ajpf-645	159	24	a	a	PRON
ajpf-645	159	25	;	;	PUNCT
ajpf-645	159	26	ompw	ompw	NOUN
ajpf-645	159	27	,	,	PUNCT
ajpf-645	159	28	outer	outer	ADJ
ajpf-645	159	29	membrane	membrane	NOUN
ajpf-645	159	30	protein	protein	NOUN
ajpf-645	159	31	w	w	PROPN
ajpf-645	159	32	;	;	PUNCT
ajpf-645	159	33	gsp	gsp	VERB
ajpf-645	159	34	,	,	PUNCT
ajpf-645	159	35	general	general	ADJ
ajpf-645	159	36	secretion	secretion	NOUN
ajpf-645	159	37	pathway	pathway	NOUN
ajpf-645	159	38	.	.	PUNCT
ajpf-645	160	1	references	reference	NOUN
ajpf-645	160	2	beha	beha	VERB
ajpf-645	160	3	d	d	NOUN
ajpf-645	160	4	,	,	PUNCT
ajpf-645	160	5	deitermann	deitermann	PROPN
ajpf-645	160	6	s	s	PROPN
ajpf-645	160	7	,	,	PUNCT
ajpf-645	160	8	muller	muller	PROPN
ajpf-645	160	9	m	m	PROPN
ajpf-645	160	10	,	,	PUNCT
ajpf-645	160	11	koch	koch	PROPN
ajpf-645	160	12	hg	hg	PROPN
ajpf-645	160	13	(	(	PUNCT
ajpf-645	160	14	2003	2003	NUM
ajpf-645	160	15	)	)	PUNCT
ajpf-645	160	16	.	.	PUNCT
ajpf-645	161	1	export	export	NOUN
ajpf-645	161	2	of	of	ADP
ajpf-645	161	3	betalactamase	betalactamase	NOUN
ajpf-645	161	4	is	be	AUX
ajpf-645	161	5	independent	independent	ADJ
ajpf-645	161	6	of	of	ADP
ajpf-645	161	7	the	the	DET
ajpf-645	161	8	signal	signal	NOUN
ajpf-645	161	9	recognition	recognition	NOUN
ajpf-645	161	10	particle	particle	NOUN
ajpf-645	161	11	.	.	PUNCT
ajpf-645	162	1	j.	j.	PROPN
ajpf-645	162	2	biol	biol	PROPN
ajpf-645	162	3	.	.	PUNCT
ajpf-645	163	1	chem	chem	PROPN
ajpf-645	163	2	.	.	PUNCT
ajpf-645	163	3	,	,	PUNCT
ajpf-645	164	1	278(24	278(24	NUM
ajpf-645	164	2	):	):	PUNCT
ajpf-645	164	3	22161	22161	NUM
ajpf-645	164	4	-	-	SYM
ajpf-645	164	5	22167	22167	NUM
ajpf-645	164	6	.	.	PUNCT
ajpf-645	165	1	bertani	bertani	PROPN
ajpf-645	165	2	g	g	PROPN
ajpf-645	165	3	(	(	PUNCT
ajpf-645	165	4	1952	1952	NUM
ajpf-645	165	5	)	)	PUNCT
ajpf-645	165	6	.	.	PUNCT
ajpf-645	166	1	studies	study	NOUN
ajpf-645	166	2	on	on	ADP
ajpf-645	166	3	lysogenesis	lysogenesis	PROPN
ajpf-645	166	4	.	.	PUNCT
ajpf-645	167	1	i.	i.	PROPN
ajpf-645	167	2	the	the	DET
ajpf-645	167	3	mode	mode	NOUN
ajpf-645	167	4	of	of	ADP
ajpf-645	167	5	phage	phage	NOUN
ajpf-645	167	6	liberation	liberation	NOUN
ajpf-645	167	7	by	by	ADP
ajpf-645	167	8	lysogenic	lysogenic	ADJ
ajpf-645	167	9	escherichia	escherichia	NOUN
ajpf-645	167	10	coli	coli	NOUN
ajpf-645	167	11	.	.	PUNCT
ajpf-645	168	1	j.	j.	PROPN
ajpf-645	168	2	bacteriol	bacteriol	PROPN
ajpf-645	168	3	.	.	PUNCT
ajpf-645	168	4	,	,	PUNCT
ajpf-645	168	5	62	62	NUM
ajpf-645	168	6	:	:	PUNCT
ajpf-645	168	7	293	293	NUM
ajpf-645	168	8	-	-	SYM
ajpf-645	168	9	300	300	NUM
ajpf-645	168	10	.	.	PUNCT
ajpf-645	169	1	briles	brile	NOUN
ajpf-645	169	2	de	de	PROPN
ajpf-645	169	3	,	,	PUNCT
ajpf-645	169	4	tart	tart	PROPN
ajpf-645	169	5	rc	rc	PROPN
ajpf-645	169	6	,	,	PUNCT
ajpf-645	169	7	swiatlo	swiatlo	NOUN
ajpf-645	169	8	e	e	NOUN
ajpf-645	169	9	,	,	PUNCT
ajpf-645	169	10	dillard	dillard	NOUN
ajpf-645	169	11	jp	jp	PROPN
ajpf-645	169	12	,	,	PUNCT
ajpf-645	169	13	smith	smith	PROPN
ajpf-645	169	14	p	p	PROPN
ajpf-645	169	15	,	,	PUNCT
ajpf-645	169	16	benton	benton	PROPN
ajpf-645	169	17	ka	ka	PROPN
ajpf-645	169	18	,	,	PUNCT
ajpf-645	169	19	ralph	ralph	PROPN
ajpf-645	169	20	ba	ba	PROPN
ajpf-645	169	21	,	,	PUNCT
ajpf-645	169	22	brooks	brooks	PROPN
ajpf-645	169	23	-	-	PUNCT
ajpf-645	169	24	walter	walter	PROPN
ajpf-645	169	25	a	a	NOUN
ajpf-645	169	26	,	,	PUNCT
ajpf-645	169	27	crain	crain	PROPN
ajpf-645	169	28	mj	mj	PROPN
ajpf-645	169	29	,	,	PUNCT
ajpf-645	169	30	hollingshead	hollingshead	ADJ
ajpf-645	169	31	sk	sk	ADP
ajpf-645	169	32	,	,	PUNCT
ajpf-645	169	33	mcdaniel	mcdaniel	PROPN
ajpf-645	169	34	ls	ls	PROPN
ajpf-645	169	35	(	(	PUNCT
ajpf-645	169	36	1998	1998	NUM
ajpf-645	169	37	)	)	PUNCT
ajpf-645	169	38	.	.	PUNCT
ajpf-645	170	1	pneumococcal	pneumococcal	ADJ
ajpf-645	170	2	diversity	diversity	NOUN
ajpf-645	170	3	:	:	PUNCT
ajpf-645	170	4	considerations	consideration	NOUN
ajpf-645	170	5	for	for	ADP
ajpf-645	170	6	new	new	ADJ
ajpf-645	170	7	vaccine	vaccine	NOUN
ajpf-645	170	8	strategies	strategy	NOUN
ajpf-645	170	9	with	with	ADP
ajpf-645	170	10	emphasis	emphasis	NOUN
ajpf-645	170	11	on	on	ADP
ajpf-645	170	12	pneumococcal	pneumococcal	ADJ
ajpf-645	170	13	surface	surface	NOUN
ajpf-645	170	14	protein	protein	NOUN
ajpf-645	170	15	a	a	DET
ajpf-645	170	16	(	(	PUNCT
ajpf-645	170	17	pspa	pspa	NOUN
ajpf-645	170	18	)	)	PUNCT
ajpf-645	170	19	.	.	PUNCT
ajpf-645	171	1	clin	clin	PROPN
ajpf-645	171	2	.	.	PUNCT
ajpf-645	172	1	microbiol	microbiol	PROPN
ajpf-645	172	2	.	.	PUNCT
ajpf-645	173	1	rev	rev	PROPN
ajpf-645	173	2	.	.	PROPN
ajpf-645	173	3	,	,	PUNCT
ajpf-645	173	4	11	11	NUM
ajpf-645	173	5	:	:	PUNCT
ajpf-645	173	6	645	645	NUM
ajpf-645	173	7	-	-	SYM
ajpf-645	173	8	657	657	NUM
ajpf-645	173	9	.	.	PUNCT
ajpf-645	174	1	driessen	driessen	PROPN
ajpf-645	174	2	aj	aj	PROPN
ajpf-645	174	3	,	,	PUNCT
ajpf-645	174	4	manting	mante	VERB
ajpf-645	174	5	eh	eh	INTJ
ajpf-645	174	6	,	,	PUNCT
ajpf-645	174	7	van	van	PROPN
ajpf-645	174	8	der	der	NOUN
ajpf-645	174	9	does	do	VERB
ajpf-645	174	10	c	c	X
ajpf-645	174	11	(	(	PUNCT
ajpf-645	174	12	2001	2001	NUM
ajpf-645	174	13	)	)	PUNCT
ajpf-645	174	14	.	.	PUNCT
ajpf-645	175	1	the	the	DET
ajpf-645	175	2	structural	structural	ADJ
ajpf-645	175	3	basis	basis	NOUN
ajpf-645	175	4	of	of	ADP
ajpf-645	175	5	protein	protein	NOUN
ajpf-645	175	6	targeting	target	VERB
ajpf-645	175	7	and	and	CCONJ
ajpf-645	175	8	translocation	translocation	NOUN
ajpf-645	175	9	in	in	ADP
ajpf-645	175	10	bacteria	bacteria	NOUN
ajpf-645	175	11	.	.	PUNCT
ajpf-645	176	1	nat	nat	PROPN
ajpf-645	176	2	.	.	PUNCT
ajpf-645	176	3	struct	struct	NOUN
ajpf-645	176	4	.	.	PUNCT
ajpf-645	177	1	biol	biol	PROPN
ajpf-645	177	2	.	.	PUNCT
ajpf-645	177	3	,	,	PUNCT
ajpf-645	177	4	8(6	8(6	NUM
ajpf-645	177	5	):	):	PUNCT
ajpf-645	177	6	492	492	NUM
ajpf-645	177	7	-	-	SYM
ajpf-645	177	8	498	498	NUM
ajpf-645	177	9	.	.	PUNCT
ajpf-645	178	1	hanahan	hanahan	PROPN
ajpf-645	178	2	d	d	PROPN
ajpf-645	178	3	(	(	PUNCT
ajpf-645	178	4	1983	1983	NUM
ajpf-645	178	5	)	)	PUNCT
ajpf-645	178	6	.	.	PUNCT
ajpf-645	179	1	studies	study	NOUN
ajpf-645	179	2	on	on	ADP
ajpf-645	179	3	transformation	transformation	NOUN
ajpf-645	179	4	of	of	ADP
ajpf-645	179	5	escherichia	escherichia	NOUN
ajpf-645	179	6	coli	coli	NOUN
ajpf-645	179	7	with	with	ADP
ajpf-645	179	8	plasmids	plasmid	NOUN
ajpf-645	179	9	.	.	PUNCT
ajpf-645	180	1	j.	j.	PROPN
ajpf-645	180	2	mol	mol	PROPN
ajpf-645	180	3	.	.	PUNCT
ajpf-645	181	1	biol	biol	PROPN
ajpf-645	181	2	.	.	PUNCT
ajpf-645	181	3	,	,	PUNCT
ajpf-645	181	4	166	166	NUM
ajpf-645	181	5	:	:	PUNCT
ajpf-645	181	6	557	557	NUM
ajpf-645	181	7	-	-	SYM
ajpf-645	181	8	580	580	NUM
ajpf-645	181	9	.	.	PUNCT
ajpf-645	182	1	hoong	hoong	PROPN
ajpf-645	182	2	yy	yy	PROPN
ajpf-645	182	3	,	,	PUNCT
ajpf-645	182	4	yusof	yusof	PROPN
ajpf-645	182	5	f	f	PROPN
ajpf-645	182	6	,	,	PUNCT
ajpf-645	182	7	abdullah	abdullah	PROPN
ajpf-645	182	8	l	l	PROPN
ajpf-645	182	9	(	(	PUNCT
ajpf-645	182	10	1995	1995	NUM
ajpf-645	182	11	)	)	PUNCT
ajpf-645	182	12	.	.	PUNCT
ajpf-645	183	1	precipitation	precipitation	NOUN
ajpf-645	183	2	of	of	ADP
ajpf-645	183	3	hevea	hevea	NOUN
ajpf-645	183	4	brasiliensis	brasiliensis	NOUN
ajpf-645	183	5	latex	latex	NOUN
ajpf-645	183	6	proteins	protein	NOUN
ajpf-645	183	7	with	with	ADP
ajpf-645	183	8	trichliroacetic	trichliroacetic	ADJ
ajpf-645	183	9	acid	acid	NOUN
ajpf-645	183	10	and	and	CCONJ
ajpf-645	183	11	phosphotungstic	phosphotungstic	ADJ
ajpf-645	183	12	acid	acid	NOUN
ajpf-645	183	13	in	in	ADP
ajpf-645	183	14	preparation	preparation	NOUN
ajpf-645	183	15	for	for	SCONJ
ajpf-645	183	16	the	the	DET
ajpf-645	183	17	lowry	lowry	NOUN
ajpf-645	183	18	protein	protein	NOUN
ajpf-645	183	19	assay	assay	VERB
ajpf-645	183	20	.	.	PUNCT
ajpf-645	184	1	analyt	analyt	PROPN
ajpf-645	184	2	.	.	PUNCT
ajpf-645	185	1	biohem	biohem	PROPN
ajpf-645	185	2	.	.	PUNCT
ajpf-645	185	3	,	,	PUNCT
ajpf-645	185	4	226	226	NUM
ajpf-645	185	5	:	:	SYM
ajpf-645	185	6	35	35	NUM
ajpf-645	185	7	-	-	SYM
ajpf-645	185	8	43	43	NUM
ajpf-645	185	9	.	.	PUNCT
ajpf-645	186	1	humphries	humphries	PROPN
ajpf-645	186	2	ad	ad	PROPN
ajpf-645	186	3	,	,	PUNCT
ajpf-645	186	4	raffatellu	raffatellu	NOUN
ajpf-645	186	5	m	m	PROPN
ajpf-645	186	6	,	,	PUNCT
ajpf-645	186	7	winter	winter	NOUN
ajpf-645	186	8	s	s	PROPN
ajpf-645	186	9	,	,	PUNCT
ajpf-645	186	10	weening	weening	NOUN
ajpf-645	186	11	eh	eh	INTJ
ajpf-645	186	12	,	,	PUNCT
ajpf-645	186	13	kingsley	kingsley	PROPN
ajpf-645	186	14	ra	ra	PROPN
ajpf-645	186	15	,	,	PUNCT
ajpf-645	186	16	droleskey	droleskey	VERB
ajpf-645	186	17	r	r	PROPN
ajpf-645	186	18	,	,	PUNCT
ajpf-645	186	19	zhang	zhang	PROPN
ajpf-645	186	20	s	s	PROPN
ajpf-645	186	21	,	,	PUNCT
ajpf-645	186	22	figueiredo	figueiredo	PROPN
ajpf-645	186	23	j	j	PROPN
ajpf-645	186	24	,	,	PUNCT
ajpf-645	186	25	khare	khare	PROPN
ajpf-645	186	26	s	s	PROPN
ajpf-645	186	27	,	,	PUNCT
ajpf-645	186	28	nunes	nunes	PROPN
ajpf-645	186	29	j	j	PROPN
ajpf-645	186	30	,	,	PUNCT
ajpf-645	186	31	adams	adams	PROPN
ajpf-645	186	32	lg	lg	PROPN
ajpf-645	186	33	,	,	PUNCT
ajpf-645	186	34	tsolis	tsolis	PROPN
ajpf-645	186	35	rm	rm	PROPN
ajpf-645	186	36	,	,	PUNCT
ajpf-645	186	37	baumler	baumler	PROPN
ajpf-645	186	38	aj	aj	PROPN
ajpf-645	186	39	(	(	PUNCT
ajpf-645	186	40	2003	2003	NUM
ajpf-645	186	41	)	)	PUNCT
ajpf-645	186	42	.	.	PUNCT
ajpf-645	187	1	the	the	DET
ajpf-645	187	2	use	use	NOUN
ajpf-645	187	3	of	of	ADP
ajpf-645	187	4	flow	flow	NOUN
ajpf-645	187	5	cytometry	cytometry	NOUN
ajpf-645	187	6	to	to	PART
ajpf-645	187	7	detect	detect	VERB
ajpf-645	187	8	expression	expression	NOUN
ajpf-645	187	9	of	of	ADP
ajpf-645	187	10	subunits	subunit	NOUN
ajpf-645	187	11	encoded	encode	VERB
ajpf-645	187	12	by	by	ADP
ajpf-645	187	13	11	11	NUM
ajpf-645	187	14	salmonella	salmonella	NOUN
ajpf-645	187	15	enterica	enterica	PROPN
ajpf-645	187	16	serotype	serotype	PROPN
ajpf-645	187	17	typhimurium	typhimurium	PROPN
ajpf-645	187	18	fimbrial	fimbrial	ADJ
ajpf-645	187	19	operons	operon	NOUN
ajpf-645	187	20	.	.	PUNCT
ajpf-645	188	1	mol	mol	PROPN
ajpf-645	188	2	.	.	PROPN
ajpf-645	188	3	microbiol	microbiol	PROPN
ajpf-645	188	4	.	.	PUNCT
ajpf-645	188	5	,	,	PUNCT
ajpf-645	189	1	48(5	48(5	NUM
ajpf-645	189	2	):	):	PUNCT
ajpf-645	189	3	1357	1357	NUM
ajpf-645	189	4	-	-	SYM
ajpf-645	189	5	1376	1376	NUM
ajpf-645	189	6	.	.	PUNCT
ajpf-645	190	1	kang	kang	PROPN
ajpf-645	190	2	hy	hy	PROPN
ajpf-645	190	3	,	,	PUNCT
ajpf-645	190	4	curtiss	curtiss	PROPN
ajpf-645	190	5	r	r	PROPN
ajpf-645	190	6	3rd	3rd	NOUN
ajpf-645	190	7	(	(	PUNCT
ajpf-645	190	8	2003	2003	NUM
ajpf-645	190	9	)	)	PUNCT
ajpf-645	190	10	.	.	PUNCT
ajpf-645	191	1	immune	immune	ADJ
ajpf-645	191	2	responses	response	NOUN
ajpf-645	191	3	dependent	dependent	ADJ
ajpf-645	191	4	on	on	ADP
ajpf-645	191	5	antigen	antigen	ADJ
ajpf-645	191	6	location	location	NOUN
ajpf-645	191	7	in	in	ADP
ajpf-645	191	8	recombinant	recombinant	ADJ
ajpf-645	191	9	attenuated	attenuate	VERB
ajpf-645	191	10	salmonella	salmonella	NOUN
ajpf-645	191	11	typhimurium	typhimurium	NOUN
ajpf-645	191	12	vaccines	vaccine	NOUN
ajpf-645	191	13	following	follow	VERB
ajpf-645	191	14	oral	oral	ADJ
ajpf-645	191	15	immunization	immunization	NOUN
ajpf-645	191	16	.	.	PUNCT
ajpf-645	192	1	fems	fems	PROPN
ajpf-645	192	2	immunol	immunol	NOUN
ajpf-645	192	3	.	.	PUNCT
ajpf-645	193	1	med	med	PROPN
ajpf-645	193	2	.	.	PUNCT
ajpf-645	193	3	microbiol	microbiol	PROPN
ajpf-645	193	4	.	.	PUNCT
ajpf-645	193	5	,	,	PUNCT
ajpf-645	194	1	37	37	NUM
ajpf-645	194	2	:	:	SYM
ajpf-645	194	3	99	99	NUM
ajpf-645	194	4	-	-	SYM
ajpf-645	194	5	104	104	NUM
ajpf-645	194	6	.	.	PUNCT
ajpf-645	195	1	kang	kang	PROPN
ajpf-645	195	2	hy	hy	PROPN
ajpf-645	195	3	,	,	PUNCT
ajpf-645	195	4	srinivasan	srinivasan	PROPN
ajpf-645	195	5	j	j	PROPN
ajpf-645	195	6	,	,	PUNCT
ajpf-645	195	7	curtiss	curtiss	PROPN
ajpf-645	195	8	r	r	PROPN
ajpf-645	195	9	3rd	3rd	NOUN
ajpf-645	195	10	(	(	PUNCT
ajpf-645	195	11	2002	2002	NUM
ajpf-645	195	12	)	)	PUNCT
ajpf-645	195	13	.	.	PUNCT
ajpf-645	196	1	immune	immune	ADJ
ajpf-645	196	2	responses	response	NOUN
ajpf-645	196	3	to	to	ADP
ajpf-645	196	4	recombinant	recombinant	ADJ
ajpf-645	196	5	pneumococcal	pneumococcal	ADJ
ajpf-645	196	6	pspa	pspa	NOUN
ajpf-645	196	7	antigen	antigen	NOUN
ajpf-645	196	8	delivered	deliver	VERB
ajpf-645	196	9	by	by	ADP
ajpf-645	196	10	live	live	ADJ
ajpf-645	196	11	attenuated	attenuate	VERB
ajpf-645	196	12	salmonella	salmonella	PROPN
ajpf-645	196	13	enterica	enterica	PROPN
ajpf-645	196	14	serovar	serovar	VERB
ajpf-645	196	15	typhimurium	typhimurium	NOUN
ajpf-645	196	16	vaccine	vaccine	NOUN
ajpf-645	196	17	.	.	PUNCT
ajpf-645	197	1	infect	infect	VERB
ajpf-645	197	2	.	.	PUNCT
ajpf-645	198	1	immun	immun	PROPN
ajpf-645	198	2	.	.	PROPN
ajpf-645	198	3	,	,	PUNCT
ajpf-645	198	4	70	70	NUM
ajpf-645	198	5	:	:	SYM
ajpf-645	198	6	1739	1739	NUM
ajpf-645	198	7	-	-	SYM
ajpf-645	198	8	1749	1749	NUM
ajpf-645	198	9	.	.	PUNCT
ajpf-645	199	1	kim	kim	PROPN
ajpf-645	199	2	sw	sw	PROPN
ajpf-645	199	3	,	,	PUNCT
ajpf-645	199	4	kim	kim	PROPN
ajpf-645	199	5	yh	yh	PROPN
ajpf-645	199	6	,	,	PUNCT
ajpf-645	199	7	yoo	yoo	PROPN
ajpf-645	199	8	ay	ay	PROPN
ajpf-645	199	9	,	,	PUNCT
ajpf-645	199	10	yu	yu	PROPN
ajpf-645	199	11	je	je	PROPN
ajpf-645	199	12	,	,	PUNCT
ajpf-645	199	13	hur	hur	PROPN
ajpf-645	199	14	j	j	PROPN
ajpf-645	199	15	,	,	PUNCT
ajpf-645	199	16	lee	lee	PROPN
ajpf-645	199	17	jh	jh	PROPN
ajpf-645	199	18	,	,	PUNCT
ajpf-645	199	19	cha	cha	PROPN
ajpf-645	199	20	jh	jh	PROPN
ajpf-645	199	21	,	,	PUNCT
ajpf-645	199	22	kang	kang	PROPN
ajpf-645	199	23	hy	hy	PROPN
ajpf-645	199	24	(	(	PUNCT
ajpf-645	199	25	2007	2007	NUM
ajpf-645	199	26	)	)	PUNCT
ajpf-645	199	27	.	.	PUNCT
ajpf-645	200	1	development	development	NOUN
ajpf-645	200	2	of	of	ADP
ajpf-645	200	3	a	a	DET
ajpf-645	200	4	protein	protein	NOUN
ajpf-645	200	5	secretion	secretion	NOUN
ajpf-645	200	6	system	system	NOUN
ajpf-645	200	7	with	with	ADP
ajpf-645	200	8	the	the	DET
ajpf-645	200	9	application	application	NOUN
ajpf-645	200	10	of	of	ADP
ajpf-645	200	11	sec	sec	PROPN
ajpf-645	200	12	dependent	dependent	ADJ
ajpf-645	200	13	protein	protein	NOUN
ajpf-645	200	14	secretion	secretion	NOUN
ajpf-645	200	15	components	component	NOUN
ajpf-645	200	16	.	.	PUNCT
ajpf-645	201	1	j.	j.	PROPN
ajpf-645	201	2	microbiol	microbiol	PROPN
ajpf-645	201	3	.	.	PUNCT
ajpf-645	202	1	biotechnol	biotechnol	PROPN
ajpf-645	202	2	.	.	PUNCT
ajpf-645	202	3	,	,	PUNCT
ajpf-645	202	4	17	17	NUM
ajpf-645	202	5	(	(	PUNCT
ajpf-645	202	6	8)	8)	NUM
ajpf-645	202	7	:	:	PUNCT
ajpf-645	202	8	1316	1316	NUM
ajpf-645	202	9	-	-	SYM
ajpf-645	202	10	1323	1323	NUM
ajpf-645	202	11	.	.	PUNCT
ajpf-645	203	1	kim	kim	PROPN
ajpf-645	203	2	sw	sw	PROPN
ajpf-645	203	3	,	,	PUNCT
ajpf-645	203	4	moon	moon	PROPN
ajpf-645	203	5	kh	kh	PROPN
ajpf-645	203	6	,	,	PUNCT
ajpf-645	203	7	baik	baik	PROPN
ajpf-645	203	8	hs	hs	PROPN
ajpf-645	203	9	,	,	PUNCT
ajpf-645	203	10	kang	kang	PROPN
ajpf-645	203	11	hy	hy	PROPN
ajpf-645	203	12	,	,	PUNCT
ajpf-645	203	13	kim	kim	PROPN
ajpf-645	203	14	sk	sk	PROPN
ajpf-645	203	15	,	,	PUNCT
ajpf-645	203	16	bahk	bahk	PROPN
ajpf-645	203	17	jd	jd	PROPN
ajpf-645	203	18	,	,	PUNCT
ajpf-645	203	19	hur	hur	PROPN
ajpf-645	203	20	j	j	PROPN
ajpf-645	203	21	,	,	PUNCT
ajpf-645	203	22	lee	lee	PROPN
ajpf-645	203	23	jh	jh	PROPN
ajpf-645	203	24	(	(	PUNCT
ajpf-645	203	25	2009	2009	NUM
ajpf-645	203	26	)	)	PUNCT
ajpf-645	203	27	.	.	PUNCT
ajpf-645	204	1	changes	change	NOUN
ajpf-645	204	2	of	of	ADP
ajpf-645	204	3	physiological	physiological	ADJ
ajpf-645	204	4	and	and	CCONJ
ajpf-645	204	5	biochemical	biochemical	ADJ
ajpf-645	204	6	properties	property	NOUN
ajpf-645	204	7	of	of	ADP
ajpf-645	204	8	salmonella	salmonella	PROPN
ajpf-645	204	9	enterica	enterica	PROPN
ajpf-645	204	10	serovar	serovar	PROPN
ajpf-645	204	11	typhimurium	typhimurium	NOUN
ajpf-645	204	12	by	by	ADP
ajpf-645	204	13	deletion	deletion	NOUN
ajpf-645	204	14	of	of	ADP
ajpf-645	204	15	cpxr	cpxr	PROPN
ajpf-645	204	16	and	and	CCONJ
ajpf-645	204	17	lon	lon	NOUN
ajpf-645	204	18	genes	gene	NOUN
ajpf-645	204	19	using	use	VERB
ajpf-645	204	20	allelic	allelic	ADJ
ajpf-645	204	21	exchange	exchange	NOUN
ajpf-645	204	22	method	method	NOUN
ajpf-645	204	23	.	.	PUNCT
ajpf-645	205	1	j.	j.	PROPN
ajpf-645	205	2	microbiol	microbiol	PROPN
ajpf-645	205	3	.	.	PUNCT
ajpf-645	206	1	methods	method	NOUN
ajpf-645	206	2	,	,	PUNCT
ajpf-645	206	3	79	79	NUM
ajpf-645	206	4	(	(	PUNCT
ajpf-645	206	5	3	3	NUM
ajpf-645	206	6	):	):	PUNCT
ajpf-645	206	7	314	314	NUM
ajpf-645	206	8	-	-	SYM
ajpf-645	206	9	320	320	NUM
ajpf-645	206	10	.	.	PUNCT
ajpf-645	207	1	koch	koch	PROPN
ajpf-645	207	2	hg	hg	PROPN
ajpf-645	207	3	,	,	PUNCT
ajpf-645	207	4	moser	moser	PROPN
ajpf-645	207	5	m	m	PROPN
ajpf-645	207	6	,	,	PUNCT
ajpf-645	207	7	muller	muller	PROPN
ajpf-645	207	8	m	m	PROPN
ajpf-645	207	9	(	(	PUNCT
ajpf-645	207	10	2003	2003	NUM
ajpf-645	207	11	)	)	PUNCT
ajpf-645	207	12	.	.	PUNCT
ajpf-645	208	1	signal	signal	PROPN
ajpf-645	208	2	recognition	recognition	NOUN
ajpf-645	208	3	particledependent	particledependent	NOUN
ajpf-645	208	4	protein	protein	NOUN
ajpf-645	208	5	targeting	target	VERB
ajpf-645	208	6	,	,	PUNCT
ajpf-645	208	7	universal	universal	ADJ
ajpf-645	208	8	to	to	ADP
ajpf-645	208	9	all	all	DET
ajpf-645	208	10	kingdoms	kingdom	NOUN
ajpf-645	208	11	of	of	ADP
ajpf-645	208	12	life	life	NOUN
ajpf-645	208	13	.	.	PUNCT
ajpf-645	209	1	rev	rev	PROPN
ajpf-645	209	2	.	.	PROPN
ajpf-645	209	3	physiol	physiol	PROPN
ajpf-645	209	4	.	.	PUNCT
ajpf-645	210	1	biochem	biochem	PROPN
ajpf-645	210	2	.	.	PUNCT
ajpf-645	211	1	pharmacol	pharmacol	PROPN
ajpf-645	211	2	.	.	PROPN
ajpf-645	212	1	,	,	PUNCT
ajpf-645	212	2	146	146	NUM
ajpf-645	212	3	:	:	SYM
ajpf-645	212	4	55	55	NUM
ajpf-645	212	5	-	-	SYM
ajpf-645	212	6	94	94	NUM
ajpf-645	212	7	.	.	PUNCT
ajpf-645	213	1	koshland	koshland	PROPN
ajpf-645	214	1	d	d	PROPN
ajpf-645	214	2	,	,	PUNCT
ajpf-645	214	3	botstein	botstein	ADJ
ajpf-645	214	4	d	d	X
ajpf-645	214	5	(	(	PUNCT
ajpf-645	214	6	1980	1980	NUM
ajpf-645	214	7	)	)	PUNCT
ajpf-645	214	8	.	.	PUNCT
ajpf-645	215	1	secretion	secretion	NOUN
ajpf-645	215	2	of	of	ADP
ajpf-645	215	3	-lactamase	-lactamase	PROPN
ajpf-645	215	4	requires	require	VERB
ajpf-645	215	5	the	the	DET
ajpf-645	215	6	carboxy	carboxy	ADJ
ajpf-645	215	7	end	end	NOUN
ajpf-645	215	8	of	of	ADP
ajpf-645	215	9	the	the	DET
ajpf-645	215	10	protein	protein	NOUN
ajpf-645	215	11	.	.	PUNCT
ajpf-645	216	1	cell	cell	NOUN
ajpf-645	216	2	,	,	PUNCT
ajpf-645	216	3	20	20	NUM
ajpf-645	216	4	:	:	SYM
ajpf-645	216	5	749	749	NUM
ajpf-645	216	6	-	-	SYM
ajpf-645	216	7	760	760	NUM
ajpf-645	216	8	.	.	PUNCT
ajpf-645	217	1	li	li	PROPN
ajpf-645	217	2	y	y	PROPN
ajpf-645	217	3	,	,	PUNCT
ajpf-645	217	4	wang	wang	PROPN
ajpf-645	217	5	s	s	PROPN
ajpf-645	217	6	,	,	PUNCT
ajpf-645	217	7	xin	xin	PROPN
ajpf-645	217	8	w	w	PROPN
ajpf-645	217	9	,	,	PUNCT
ajpf-645	217	10	scarpellini	scarpellini	ADP
ajpf-645	217	11	g	g	PROPN
ajpf-645	217	12	,	,	PUNCT
ajpf-645	217	13	shi	shi	PROPN
ajpf-645	217	14	z	z	PROPN
ajpf-645	217	15	,	,	PUNCT
ajpf-645	217	16	gunn	gunn	PROPN
ajpf-645	217	17	b	b	PROPN
ajpf-645	217	18	,	,	PUNCT
ajpf-645	217	19	roland	roland	PROPN
ajpf-645	217	20	kl	kl	PROPN
ajpf-645	217	21	,	,	PUNCT
ajpf-645	217	22	curtiss	curtiss	PROPN
ajpf-645	217	23	r	r	PROPN
ajpf-645	217	24	3rd	3rd	NOUN
ajpf-645	217	25	(	(	PUNCT
ajpf-645	217	26	2008	2008	NUM
ajpf-645	217	27	)	)	PUNCT
ajpf-645	217	28	.	.	PUNCT
ajpf-645	218	1	a	a	DET
ajpf-645	218	2	sopb	sopb	ADJ
ajpf-645	218	3	deletion	deletion	NOUN
ajpf-645	218	4	mutation	mutation	NOUN
ajpf-645	218	5	enhances	enhance	VERB
ajpf-645	218	6	the	the	DET
ajpf-645	218	7	immunogenicity	immunogenicity	NOUN
ajpf-645	218	8	and	and	CCONJ
ajpf-645	218	9	protective	protective	ADJ
ajpf-645	218	10	efficacy	efficacy	NOUN
ajpf-645	218	11	of	of	ADP
ajpf-645	218	12	a	a	DET
ajpf-645	218	13	heterologous	heterologous	ADJ
ajpf-645	218	14	antigen	antigen	NOUN
ajpf-645	218	15	delivered	deliver	VERB
ajpf-645	218	16	by	by	ADP
ajpf-645	218	17	live	live	ADJ
ajpf-645	218	18	attenuated	attenuate	VERB
ajpf-645	218	19	salmonella	salmonella	NOUN
ajpf-645	218	20	enterica	enterica	PROPN
ajpf-645	218	21	vaccines	vaccine	NOUN
ajpf-645	218	22	.	.	PUNCT
ajpf-645	219	1	infect	infect	VERB
ajpf-645	219	2	.	.	PUNCT
ajpf-645	220	1	immun	immun	PROPN
ajpf-645	220	2	.	.	PROPN
ajpf-645	220	3	,	,	PUNCT
ajpf-645	220	4	76	76	NUM
ajpf-645	220	5	:	:	PUNCT
ajpf-645	220	6	5238	5238	NUM
ajpf-645	220	7	-	-	SYM
ajpf-645	220	8	5246	5246	NUM
ajpf-645	220	9	.	.	PUNCT
ajpf-645	221	1	ma	ma	PROPN
ajpf-645	221	2	j	j	PROPN
ajpf-645	221	3	,	,	PUNCT
ajpf-645	221	4	stoter	stoter	NOUN
ajpf-645	221	5	g	g	PROPN
ajpf-645	221	6	,	,	PUNCT
ajpf-645	221	7	verweij	verweij	PROPN
ajpf-645	221	8	j	j	PROPN
ajpf-645	221	9	,	,	PUNCT
ajpf-645	221	10	schellens	schellen	VERB
ajpf-645	221	11	jh	jh	PROPN
ajpf-645	221	12	(	(	PUNCT
ajpf-645	221	13	1996	1996	NUM
ajpf-645	221	14	)	)	PUNCT
ajpf-645	221	15	.	.	PUNCT
ajpf-645	222	1	comparison	comparison	NOUN
ajpf-645	222	2	of	of	ADP
ajpf-645	222	3	ethanol	ethanol	NOUN
ajpf-645	222	4	plasma	plasma	NOUN
ajpf-645	222	5	-	-	PUNCT
ajpf-645	222	6	protein	protein	NOUN
ajpf-645	222	7	precipitation	precipitation	NOUN
ajpf-645	222	8	with	with	ADP
ajpf-645	222	9	plasma	plasma	NOUN
ajpf-645	222	10	ultrafiltration	ultrafiltration	NOUN
ajpf-645	222	11	and	and	CCONJ
ajpf-645	222	12	trichloroacetic	trichloroacetic	ADJ
ajpf-645	222	13	acid	acid	NOUN
ajpf-645	222	14	protein	protein	NOUN
ajpf-645	222	15	precipitations	precipitation	NOUN
ajpf-645	222	16	for	for	ADP
ajpf-645	222	17	the	the	DET
ajpf-645	222	18	measurement	measurement	NOUN
ajpf-645	222	19	of	of	ADP
ajpf-645	222	20	unbound	unbound	NOUN
ajpf-645	222	21	platinum	platinum	NOUN
ajpf-645	222	22	concentrations	concentration	NOUN
ajpf-645	222	23	.	.	PUNCT
ajpf-645	223	1	cancer	cancer	NOUN
ajpf-645	223	2	chemother	chemother	PROPN
ajpf-645	223	3	.	.	PUNCT
ajpf-645	224	1	pharmacol	pharmacol	PROPN
ajpf-645	224	2	.	.	PROPN
ajpf-645	224	3	,	,	PUNCT
ajpf-645	224	4	38	38	NUM
ajpf-645	224	5	:	:	SYM
ajpf-645	224	6	391	391	NUM
ajpf-645	224	7	-	-	SYM
ajpf-645	224	8	394	394	NUM
ajpf-645	224	9	.	.	PUNCT
ajpf-645	225	1	majander	majander	PROPN
ajpf-645	225	2	k	k	PROPN
ajpf-645	225	3	,	,	PUNCT
ajpf-645	225	4	anton	anton	PROPN
ajpf-645	225	5	l	l	PROPN
ajpf-645	225	6	,	,	PUNCT
ajpf-645	225	7	antikainen	antikainen	PROPN
ajpf-645	225	8	j	j	PROPN
ajpf-645	225	9	,	,	PUNCT
ajpf-645	225	10	lang	lang	PROPN
ajpf-645	225	11	h	h	PROPN
ajpf-645	225	12	,	,	PUNCT
ajpf-645	225	13	brummer	brummer	NOUN
ajpf-645	225	14	m	m	PROPN
ajpf-645	225	15	,	,	PUNCT
ajpf-645	225	16	korhonen	korhonen	PROPN
ajpf-645	225	17	tk	tk	PROPN
ajpf-645	225	18	,	,	PUNCT
ajpf-645	225	19	westerlund	westerlund	PROPN
ajpf-645	225	20	-	-	PUNCT
ajpf-645	225	21	wikstrom	wikstrom	NOUN
ajpf-645	225	22	b	b	PROPN
ajpf-645	225	23	(	(	PUNCT
ajpf-645	225	24	2005a	2005a	NUM
ajpf-645	225	25	)	)	PUNCT
ajpf-645	225	26	.	.	PUNCT
ajpf-645	226	1	extracellular	extracellular	ADJ
ajpf-645	226	2	secretion	secretion	NOUN
ajpf-645	226	3	of	of	ADP
ajpf-645	226	4	polypeptides	polypeptide	NOUN
ajpf-645	226	5	using	use	VERB
ajpf-645	226	6	a	a	DET
ajpf-645	226	7	modified	modify	VERB
ajpf-645	226	8	escherichia	escherichia	NOUN
ajpf-645	226	9	coli	coli	NOUN
ajpf-645	226	10	flagellar	flagellar	ADJ
ajpf-645	226	11	secretion	secretion	NOUN
ajpf-645	226	12	apparatus	apparatus	NOUN
ajpf-645	226	13	.	.	PUNCT
ajpf-645	227	1	nat	nat	PROPN
ajpf-645	227	2	.	.	PUNCT
ajpf-645	228	1	biotechnol	biotechnol	PROPN
ajpf-645	228	2	.	.	PUNCT
ajpf-645	228	3	,	,	PUNCT
ajpf-645	228	4	23	23	NUM
ajpf-645	228	5	:	:	SYM
ajpf-645	228	6	475	475	NUM
ajpf-645	228	7	-	-	SYM
ajpf-645	228	8	481	481	NUM
ajpf-645	228	9	.	.	PUNCT
ajpf-645	229	1	majander	majander	PROPN
ajpf-645	229	2	k	k	PROPN
ajpf-645	229	3	,	,	PUNCT
ajpf-645	229	4	korhonen	korhonen	PROPN
ajpf-645	229	5	tk	tk	PROPN
ajpf-645	229	6	,	,	PUNCT
ajpf-645	229	7	westerlund	westerlund	PROPN
ajpf-645	229	8	-	-	PUNCT
ajpf-645	229	9	wikstrom	wikstrom	NOUN
ajpf-645	229	10	b	b	PROPN
ajpf-645	229	11	(	(	PUNCT
ajpf-645	229	12	2005b	2005b	NUM
ajpf-645	229	13	)	)	PUNCT
ajpf-645	229	14	.	.	PUNCT
ajpf-645	230	1	simultaneous	simultaneous	ADJ
ajpf-645	230	2	display	display	NOUN
ajpf-645	230	3	of	of	ADP
ajpf-645	230	4	multiple	multiple	ADJ
ajpf-645	230	5	foreign	foreign	ADJ
ajpf-645	230	6	peptides	peptide	NOUN
ajpf-645	230	7	in	in	ADP
ajpf-645	230	8	the	the	DET
ajpf-645	230	9	flid	flid	NOUN
ajpf-645	230	10	capping	capping	NOUN
ajpf-645	230	11	and	and	CCONJ
ajpf-645	230	12	flic	flic	ADJ
ajpf-645	230	13	filament	filament	NOUN
ajpf-645	230	14	proteins	protein	NOUN
ajpf-645	230	15	of	of	ADP
ajpf-645	230	16	the	the	DET
ajpf-645	230	17	escherichia	escherichia	NOUN
ajpf-645	230	18	coli	coli	NOUN
ajpf-645	230	19	flagellum	flagellum	NOUN
ajpf-645	230	20	.	.	PUNCT
ajpf-645	231	1	appl	appl	PROPN
ajpf-645	231	2	.	.	PUNCT
ajpf-645	232	1	environ	environ	PROPN
ajpf-645	232	2	.	.	PUNCT
ajpf-645	232	3	microbiol	microbiol	PROPN
ajpf-645	232	4	.	.	PUNCT
ajpf-645	232	5	,	,	PUNCT
ajpf-645	233	1	71	71	NUM
ajpf-645	233	2	:	:	SYM
ajpf-645	233	3	4263	4263	NUM
ajpf-645	233	4	-	-	SYM
ajpf-645	233	5	4268	4268	NUM
ajpf-645	233	6	.	.	PUNCT
ajpf-645	234	1	mcdaniel	mcdaniel	PROPN
ajpf-645	234	2	ls	ls	PROPN
ajpf-645	234	3	,	,	PUNCT
ajpf-645	234	4	scott	scott	PROPN
ajpf-645	234	5	g	g	PROPN
ajpf-645	234	6	,	,	PUNCT
ajpf-645	234	7	kearney	kearney	PROPN
ajpf-645	234	8	jf	jf	PROPN
ajpf-645	234	9	,	,	PUNCT
ajpf-645	234	10	briles	brile	NOUN
ajpf-645	234	11	de	de	X
ajpf-645	234	12	(	(	PUNCT
ajpf-645	234	13	1984	1984	NUM
ajpf-645	234	14	)	)	PUNCT
ajpf-645	234	15	.	.	PUNCT
ajpf-645	235	1	monoclonal	monoclonal	NOUN
ajpf-645	235	2	antibodies	antibody	NOUN
ajpf-645	235	3	against	against	ADP
ajpf-645	235	4	protease	protease	NOUN
ajpf-645	235	5	sensitive	sensitive	ADJ
ajpf-645	235	6	pneumococcal	pneumococcal	ADJ
ajpf-645	235	7	antigens	antigen	NOUN
ajpf-645	235	8	can	can	AUX
ajpf-645	235	9	protect	protect	VERB
ajpf-645	235	10	mice	mouse	NOUN
ajpf-645	235	11	from	from	ADP
ajpf-645	235	12	fatal	fatal	ADJ
ajpf-645	235	13	infection	infection	NOUN
ajpf-645	235	14	with	with	ADP
ajpf-645	235	15	streptococcus	streptococcus	NOUN
ajpf-645	235	16	pneumoniae	pneumoniae	NOUN
ajpf-645	235	17	.	.	PUNCT
ajpf-645	236	1	j.	j.	PROPN
ajpf-645	236	2	exp	exp	PROPN
ajpf-645	236	3	.	.	PROPN
ajpf-645	236	4	med	med	PROPN
ajpf-645	236	5	.	.	PROPN
ajpf-645	236	6	,	,	PUNCT
ajpf-645	236	7	160	160	NUM
ajpf-645	236	8	:	:	PUNCT
ajpf-645	236	9	386	386	NUM
ajpf-645	236	10	-	-	SYM
ajpf-645	236	11	397	397	NUM
ajpf-645	236	12	.	.	PUNCT
ajpf-645	237	1	meeusen	meeusen	PROPN
ajpf-645	237	2	en	en	PROPN
ajpf-645	237	3	,	,	PUNCT
ajpf-645	237	4	walker	walker	PROPN
ajpf-645	237	5	j	j	PROPN
ajpf-645	237	6	,	,	PUNCT
ajpf-645	237	7	peters	peters	PROPN
ajpf-645	237	8	a	a	PRON
ajpf-645	237	9	,	,	PUNCT
ajpf-645	237	10	pastoret	pastoret	ADP
ajpf-645	237	11	pp	pp	ADV
ajpf-645	237	12	,	,	PUNCT
ajpf-645	237	13	jungersen	jungersen	NOUN
ajpf-645	237	14	g	g	PROPN
ajpf-645	237	15	(	(	PUNCT
ajpf-645	237	16	2007	2007	NUM
ajpf-645	237	17	)	)	PUNCT
ajpf-645	237	18	.	.	PUNCT
ajpf-645	238	1	current	current	ADJ
ajpf-645	238	2	status	status	NOUN
ajpf-645	238	3	of	of	ADP
ajpf-645	238	4	veterinary	veterinary	ADJ
ajpf-645	238	5	vaccines	vaccine	NOUN
ajpf-645	238	6	.	.	PUNCT
ajpf-645	239	1	clin	clin	PROPN
ajpf-645	239	2	.	.	PUNCT
ajpf-645	240	1	microbiol	microbiol	PROPN
ajpf-645	240	2	.	.	PUNCT
ajpf-645	241	1	rev	rev	PROPN
ajpf-645	241	2	.	.	PROPN
ajpf-645	241	3	,	,	PUNCT
ajpf-645	241	4	20(3	20(3	NUM
ajpf-645	241	5	):	):	PUNCT
ajpf-645	241	6	489	489	NUM
ajpf-645	241	7	-	-	SYM
ajpf-645	241	8	510	510	NUM
ajpf-645	241	9	.	.	PUNCT
ajpf-645	242	1	nakayama	nakayama	PROPN
ajpf-645	242	2	k	k	PROPN
ajpf-645	242	3	,	,	PUNCT
ajpf-645	242	4	kelly	kelly	PROPN
ajpf-645	242	5	sm	sm	PROPN
ajpf-645	242	6	,	,	PUNCT
ajpf-645	242	7	curtiss	curtiss	PROPN
ajpf-645	242	8	r	r	PROPN
ajpf-645	242	9	3rd	3rd	NOUN
ajpf-645	242	10	(	(	PUNCT
ajpf-645	242	11	1988	1988	NUM
ajpf-645	242	12	)	)	PUNCT
ajpf-645	242	13	.	.	PUNCT
ajpf-645	243	1	construction	construction	NOUN
ajpf-645	243	2	of	of	ADP
ajpf-645	243	3	an	an	DET
ajpf-645	243	4	asd	asd	NOUN
ajpf-645	243	5	+	+	CCONJ
ajpf-645	243	6	expression	expression	NOUN
ajpf-645	243	7	-	-	PUNCT
ajpf-645	243	8	cloning	cloning	NOUN
ajpf-645	243	9	vector	vector	NOUN
ajpf-645	243	10	:	:	PUNCT
ajpf-645	243	11	stable	stable	ADJ
ajpf-645	243	12	maintenance	maintenance	NOUN
ajpf-645	243	13	and	and	CCONJ
ajpf-645	243	14	high	high	ADJ
ajpf-645	243	15	level	level	NOUN
ajpf-645	243	16	expression	expression	NOUN
ajpf-645	243	17	of	of	ADP
ajpf-645	243	18	cloned	cloned	ADJ
ajpf-645	243	19	genes	gene	NOUN
ajpf-645	243	20	in	in	ADP
ajpf-645	243	21	a	a	DET
ajpf-645	243	22	salmonella	salmonella	NOUN
ajpf-645	243	23	vaccine	vaccine	NOUN
ajpf-645	243	24	strain	strain	VERB
ajpf-645	243	25	.	.	PUNCT
ajpf-645	244	1	bio	bio	NOUN
ajpf-645	244	2	/	/	SYM
ajpf-645	244	3	technology	technology	NOUN
ajpf-645	244	4	,	,	PUNCT
ajpf-645	244	5	6	6	NUM
ajpf-645	244	6	:	:	PUNCT
ajpf-645	244	7	693	693	NUM
ajpf-645	244	8	-	-	SYM
ajpf-645	244	9	697	697	NUM
ajpf-645	244	10	.	.	PUNCT
ajpf-645	245	1	sambrook	sambrook	PROPN
ajpf-645	245	2	j	j	PROPN
ajpf-645	245	3	,	,	PUNCT
ajpf-645	245	4	fritsch	fritsch	PROPN
ajpf-645	245	5	ef	ef	PROPN
ajpf-645	245	6	,	,	PUNCT
ajpf-645	245	7	maniatis	maniatis	PROPN
ajpf-645	245	8	t	t	PROPN
ajpf-645	245	9	(	(	PUNCT
ajpf-645	245	10	1989	1989	NUM
ajpf-645	245	11	)	)	PUNCT
ajpf-645	245	12	.	.	PUNCT
ajpf-645	246	1	molecular	molecular	ADJ
ajpf-645	246	2	cloning	cloning	NOUN
ajpf-645	246	3	:	:	PUNCT
ajpf-645	246	4	a	a	DET
ajpf-645	246	5	laboratory	laboratory	NOUN
ajpf-645	246	6	manual	manual	NOUN
ajpf-645	246	7	,	,	PUNCT
ajpf-645	246	8	2nd	2nd	ADJ
ajpf-645	246	9	ed	ed	NOUN
ajpf-645	246	10	.	.	PUNCT
ajpf-645	247	1	cold	cold	ADJ
ajpf-645	247	2	spring	spring	PROPN
ajpf-645	247	3	harbor	harbor	PROPN
ajpf-645	247	4	laboratory	laboratory	PROPN
ajpf-645	247	5	press	press	PROPN
ajpf-645	247	6	,	,	PUNCT
ajpf-645	247	7	cold	cold	ADJ
ajpf-645	247	8	spring	spring	NOUN
ajpf-645	247	9	harbor	harbor	NOUN
ajpf-645	247	10	,	,	PUNCT
ajpf-645	247	11	n.y	n.y	PROPN
ajpf-645	247	12	.	.	PROPN
ajpf-645	247	13	spreng	spreng	PROPN
ajpf-645	247	14	s	s	PROPN
ajpf-645	247	15	,	,	PUNCT
ajpf-645	247	16	dietrich	dietrich	PROPN
ajpf-645	247	17	g	g	PROPN
ajpf-645	247	18	,	,	PUNCT
ajpf-645	247	19	goebel	goebel	PROPN
ajpf-645	247	20	w	w	PROPN
ajpf-645	247	21	,	,	PUNCT
ajpf-645	247	22	gentschev	gentschev	VERB
ajpf-645	247	23	i	i	PRON
ajpf-645	247	24	(	(	PUNCT
ajpf-645	247	25	1999	1999	NUM
ajpf-645	247	26	)	)	PUNCT
ajpf-645	247	27	.	.	PUNCT
ajpf-645	248	1	the	the	DET
ajpf-645	248	2	escherichia	escherichia	NOUN
ajpf-645	248	3	coli	coli	NOUN
ajpf-645	248	4	haemolysin	haemolysin	NOUN
ajpf-645	248	5	secretion	secretion	NOUN
ajpf-645	248	6	apparatus	apparatus	NOUN
ajpf-645	248	7	:	:	PUNCT
ajpf-645	248	8	a	a	DET
ajpf-645	248	9	potential	potential	ADJ
ajpf-645	248	10	universal	universal	ADJ
ajpf-645	248	11	antigen	antigen	ADJ
ajpf-645	248	12	delivery	delivery	NOUN
ajpf-645	248	13	system	system	NOUN
ajpf-645	248	14	in	in	ADP
ajpf-645	248	15	gram	gram	PROPN
ajpf-645	248	16	negative	negative	ADJ
ajpf-645	248	17	bacterial	bacterial	ADJ
ajpf-645	248	18	vaccine	vaccine	NOUN
ajpf-645	248	19	carriers	carrier	NOUN
ajpf-645	248	20	.	.	PUNCT
ajpf-645	249	1	mol	mol	PROPN
ajpf-645	249	2	.	.	PROPN
ajpf-645	249	3	microbiol	microbiol	PROPN
ajpf-645	249	4	.	.	PUNCT
ajpf-645	250	1	,	,	PUNCT
ajpf-645	250	2	31	31	NUM
ajpf-645	250	3	:	:	SYM
ajpf-645	250	4	1596	1596	NUM
ajpf-645	250	5	-	-	SYM
ajpf-645	250	6	1598	1598	NUM
ajpf-645	250	7	.	.	PUNCT
ajpf-645	251	1	wong	wong	PROPN
ajpf-645	251	2	rs	rs	PROPN
ajpf-645	251	3	,	,	PUNCT
ajpf-645	251	4	wirtz	wirtz	PROPN
ajpf-645	251	5	ra	ra	PROPN
ajpf-645	251	6	,	,	PUNCT
ajpf-645	251	7	hancock	hancock	PROPN
ajpf-645	251	8	re	re	X
ajpf-645	251	9	(	(	PUNCT
ajpf-645	251	10	1995	1995	NUM
ajpf-645	251	11	)	)	PUNCT
ajpf-645	251	12	.	.	PUNCT
ajpf-645	252	1	pseudomonas	pseudomona	VERB
ajpf-645	252	2	aeruginosa	aeruginosa	PROPN
ajpf-645	252	3	outer	outer	ADJ
ajpf-645	252	4	membrane	membrane	NOUN
ajpf-645	252	5	protein	protein	NOUN
ajpf-645	252	6	oprf	oprf	NOUN
ajpf-645	252	7	as	as	ADP
ajpf-645	252	8	an	an	DET
ajpf-645	252	9	expression	expression	NOUN
ajpf-645	252	10	vector	vector	NOUN
ajpf-645	252	11	for	for	ADP
ajpf-645	252	12	foreign	foreign	ADJ
ajpf-645	252	13	epitopes	epitope	NOUN
ajpf-645	252	14	:	:	PUNCT
ajpf-645	252	15	the	the	DET
ajpf-645	252	16	effects	effect	NOUN
ajpf-645	252	17	of	of	ADP
ajpf-645	252	18	positioning	positioning	NOUN
ajpf-645	252	19	and	and	CCONJ
ajpf-645	252	20	length	length	NOUN
ajpf-645	252	21	on	on	ADP
ajpf-645	252	22	the	the	DET
ajpf-645	252	23	antigenicity	antigenicity	NOUN
ajpf-645	252	24	of	of	ADP
ajpf-645	252	25	the	the	DET
ajpf-645	252	26	epitope	epitope	NOUN
ajpf-645	252	27	.	.	PUNCT
ajpf-645	253	1	gene	gene	NOUN
ajpf-645	253	2	,	,	PUNCT
ajpf-645	253	3	158	158	NUM
ajpf-645	253	4	:	:	SYM
ajpf-645	253	5	55	55	NUM
ajpf-645	253	6	-	-	SYM
ajpf-645	253	7	60	60	NUM
ajpf-645	253	8	.	.	PUNCT