A G R I C U LT U R A L A N D F O O D S C I E N C E Agricultural and Food Science (2021) 30: 24–35 24 https://doi.org/10.23986/afsci.100693 Comparing spring triticale varieties to barley and wheat varieties when harvested as whole crop 1Katariina Manni, 2Timo Lötjönen and 3Arto Huuskonen 1Natural Resources Institute Finland (Luke), Production Systems, Tietotie 2 C, FI-31600 Jokioinen, Finland 2Natural Resources Institute Finland (Luke), Production Systems, Paavo Havaksentie 3, FI-90570 Oulu, Finland 3Natural Resources Institute Finland (Luke), Production Systems, Halolantie 31 A, FI-71750 Maaninka, Finland e-mail: katariina.manni@luke.fi Two plot experiments in two consecutive years were conducted to compare spring triticale varieties to spring barley and spring wheat varieties to produce whole crop cereals for ruminants. In the first year, triticale varieties Nagano and Nilex, barley varieties Kaarle and Trekker and the wheat variety Helmi were studied. In the second year, the triticale varieties Bikini and Somtri and the barley variety Armas were also used. The crops were harvested at the early dough stage of ripening. A treatment with reduced fertilizer and seed rates were included to study their ef- fects on the development of the undersown ley, but such effects could not be demonstrated. The triticale varieties except for Bikini produced high dry matter yields. The highest ear:stem ratio and pepsin-cellulase solubility in the ear were found in the barley varieties Armas and Kaarle, but the digestibility of the whole crop was not improved because of low pepsin-cellulase solubility of the stems or leaves. All the studied varieties were suitable for whole crop production. The production costs of the different types of whole crop silage were fairly similar. Key words: feed, forage, X Triticosecale, Hordeum vulgare, Triticum aestivum, digestibility Introduction Small grain cereal-based whole crop silage provides an opportunity to improve the efficiency of forage production for ruminants under Nordic conditions (Rustas 2009). They can be used for both dairy and beef cattle by mixing them with grass silage or as a sole forage, depending on the quality of feed and nutrient requirements of the cat- tle (Keady 2005, Huuskonen and Joki-Tokola 2010). Whole crop silage has the potential to lower costs, which has increased the interest in using them for cattle feeding. A high dry matter (DM) yield at a single harvest is one ad- vantage which reduces the costs compared to grass silage. Additionally, spring cereals can be grown in different climatic and soil conditions, and as annual crops there is no risk of a winter kill. They also can be used as nurse crops for undersown new leys. The advantage of this is that early harvesting of the whole crop promotes grass growth in the autumn. Cereals fit well in crop rotations at grass dominated farms, provide opportunities for manure spreading in spring, and the same harvesting and ensiling technology as for grasses can be used. The digestibility of whole crop silage is typically lower than that of grass silage, which may limit the use in feeding, but a higher DM intake may compensate for this (Sinclair et al. 2003, Keady 2005, Huhtanen et al. 2007, Huuskonen et al. 2020). Barley (Hordeum vulgare), oats (Avena sativa), and wheat (Triticum aestivum) are commonly used for the produc- tion of whole crop silage in Nordic conditions. Triticale (X Triticosecale) is commonly grown for grain production in Europe up to Denmark (Peltonen-Sainio et al. 2009), but it is a fairly new crop in Finland. Its high DM yield po- tential has recently increased the interest in cultivating it. However, because triticale requires rather long grow- ing season for ripening, it is commonly harvested at an earlier growth stage as whole crop silage instead of har- vesting the grains in northern regions. In an experiment by Nadeau (2007), winter triticale and spring wheat had a greater DM yield than oats and barley when harvested as a whole crop at the early dough stage of maturity. When comparing small grain cereal species, they can vary in the proportion of morphological fractions (Khorasani et al. 1997) and feed quality (Cherney and Marten 1982, Nadeau 2007, Huuskonen et al. 2017, Huuskonen et al. 2020). Khorasani et al. (1997) found that spring triticale had the highest stem proportion, but the ear proportion did not differ when compared to barley, oats and crop mixtures of barley and winter triticale. The leaf proportion of triticale was lower than for barley and crop mixtures. In previous experiments it has been found a higher digest- ibility for barley than triticale and wheat (Cherney and Marten 1982, Huuskonen et al. 2017, Huuskonen et al. 2020) but Nadeau (2007) observed that both barley and triticale had higher in vitro digestibility than oats and wheat. Also, differences in chemical composition of different whole crop silages, like barley, triticale, oats and wheat, has been found (Nadeau 2007, Huuskonen et al. 2017, Huuskonen et al. 2020). Received 20 December 2020/Accepted 18 February 2021 The Scientific Agricultural Society of Finland ©This is an open access article under the CC BY 4.0 K. Manni et al. 25 Many experiments have been conducted to evaluate the potential of small grain cereals as a raw material for whole crop silage, but there is lack of information on the yields and feed quality of spring triticale. Furthermore, there is inadequate information concerning the differences between crop varieties in terms of the yield and feed quality, especially for spring triticale varieties when used as a raw material for whole crop silage production. The objective of this experiment was to compare triticale, barley, and wheat varieties as a raw material for whole crop silage when harvested at an early dough stage of ripening. Yield, nutritive values, and fractions of the stem, leaves and ears were determined. Furthermore, the effects on establishing new leys were evaluated when cereal crops were used as the nurse crops for undersown leys under normal and reduced seed and fertilization rates and harvested as a whole crop. In addition, there is lack of information whether the production costs of different type of whole crop silages differ from costs of grass silage in typical Finnish production systems. For this need the production costs were calculated for the various types of whole crop silage. It was hypothesized that triticale varieties would produce higher whole crop yields than varieties of barley and wheat. It was also hypothesized that reduced seed and fertilization rates could prevent crop lodging and promote grass growth. Material and methods Plant material and experimental design The plot experiments were conducted during two growing seasons, in 2017 and 2018, at the Research Station of the Natural Resources Institute Finland (Luke) in Ruukki (64°44’N, 25°15’E). The soil type was sandy loam. In 2017, two varieties of triticale (Nagano, Nilex), two varieties of barley (Kaarle, Trekker), and one variety of wheat (Helmi) were used. In 2018, four triticale varieties (Bikini, Nagano, Nilex, Somtri), three barley varieties (Armas, Kaarle, Trekker), and one wheat variety (Helmi) were used. All varieties were spring types. The aim was to study such varieties which are potential for whole crop production and which are suitable for growing in northern climate conditions. The recommendations of the plant breeders and seed companies were taken into account when the varieties were chosen. The experimental design with four replicates was a split-plot in 2017, and a ran- domized complete block in 2018. The plot representing the plant variety was 1.5 × 10 m. The experiment was sown on 24 May 2017 and on 5 June 2018. The seed rate was 500 seeds m-2 for all varieties, with the exception of the Helmi wheat variety which had a seed rate of 650 seeds m-2. In 2017, the soil pH was 6.1 and the concentrations of calcium (Ca), potassium (K), phosphorus (P), magnesium (Mg) and sulfur (S) were 1400, 75, 14, 96 and 10 mg l-1, respectively. In 2018, the soil pH was 6.2 and the concen- trations of Ca, K, P, Mg and S were 1600, 110, 15, 130 and 12 mg l-1, respectively. As the values showed insuffi- cient contents of K in the soil, a K rich fertilizer was used. Artificial NPK fertilizer was applied during sowing with 90 kg N ha-1, 9 kg P ha-1 and 54 kg K ha-1. The plots were rolled after sowing. In 2017, weeds were controlled with a mixture of sulphonyl ureas and MCPA. In 2018 weed control was not necessary. Insect or plant disease control was not needed, and plant growth regulators were not used. The crops were harvested at 5 cm stubble height at the early dough stage of ripening (growth stage Z83; Zadoks et al. 1974) using a Haldrup plot harvester equipped with a scale. In 2017 and 2018, all varieties were harvested in August (Table 1). Table 1. Harvest dates for whole crop cereals harvested at the early dough stage in 2017 and 2018. The crops were sown on 24 May 2017 and 5 June 2018. Temperature sums (degree days, DD +5 ⁰C) between sowing and harvest are presented (source: Finnish Meteorological Institute). Crop species Crop variety Harvest date Temperature sum (DD), ⁰C 2017 2018 2017 2018 Barley Armas - 10.8. 788 Barley Kaarle 16.8. 10.8. 685 788 Barley Trekker 21.8. 10.8. 738 788 Wheat Helmi 25.8. 21.8. 760 892 Triticale Bikini - 27.8. 940 Triticale Nagano 29.8. 27.8. 780 940 Triticale Nilex 29.8. 27.8. 780 940 Triticale Somtri - 27.8. 940 Agricultural and Food Science (2021) 30: 24–35 26 Establishing a new ley using cereals as nurse crops at normal and reduced fertilization and seed rate was also stud- ied. In 2017, both fertilizer and seed rate were reduced by 30% of the normal rates reported above and they were analyzed as one treatment. An NPK fertilizer was applied at 63 kg N ha-1 and the seed rate was 350 seeds m-2, with the exception of the Helmi variety which was sown at a seed rate of 455 seeds m-2. The ley was established at the same time with sowing crops. The seed mixture contained timothy (Phleum pratense), meadow fescue (Festuca pratensis), and red clover (Trifolium pratense), and their seed rates were 15, 5 and 5 kg ha-1, respectively. In 2018, the primary growth and regrowth of grass was harvested on 26 June and 21 August, respectively, using a Haldrup plot harvester and at both harvests, the biomass yield was determined. The DM yield of the primary growth and regrowth were analyzed separately, and also the total DM yield was analyzed. Production costs were calculated for whole crop barley silage, triticale silage, faba bean-wheat silage, and clo- ver-timothy silage. The production costs were calculated by utilizing model calculations by ProAgria Advisory Ser- vices and contractor prices for the machine work collected by TTS (Palva 2019, ProAgria 2019). The yield ranges of the current study were used for the whole crop cereals, while those by Hakala et al. (2012) were used for grass silage, and those by Kuoppala et al. (2014, 2015) were used for faba bean-wheat silage. The used DM yields in the calculations represented fresh whole crop DM yields without harvest, storage and ensiling losses. Clover-timothy ley was assumed to be undersown with the nurse crop and it was expected to be grown for three years. The costs of establishing ley included seed costs but not machinery costs. Faba bean-wheat crop and grass were assumed to be harvested with a disc mower and pick up forage harvester, while barley and triti- cale crops was assumed to have been cut with a direct cut forage harvester. The direct cut harvester has the low- est harvest losses when harvesting grain or pulse crops, but faba bean whole crop is usually so wet that a better silage quality is achieved when the swaths are allowed to wilt on the field before ensiling (Kuoppala et al. 2014, 2015). All types of silage were assumed to be stored in clamp silos. The machine work, including transportation of the feeds, filling and sealing of silo, were assumed to have been carried out by contractors and they were not included the calculation. The interest on the working capital was calculated based on variable costs assuming that the capital was invested in production for half a year (50%) and by using a 5% interest level. Feed analyses and measurements During harvesting, the yield of all plots was weighed and samples were taken for chemical analyses. The samples were dried at 60 °C for three days to determine the DM concentration and for later analyses. Ash, crude protein (CP), neutral detergent fiber (NDF), indigestible NDF (iNDF), water-soluble carbohydrates (WSC), and digestible or- ganic matter (DOM) in the DM (DOMD, D-value) were analyzed using the near infrared (NIR) spectroscopy (FOSS NIRSystems 6500 spectrometer, Denmark) at the laboratory of Valio Ltd. Seinäjoki, Finland. The device was cali- brated for measuring chemical composition of whole crop silages. The metabolizable energy (ME) concentration of the samples was calculated as ME (MJ kg-1 DM) = 15.5 × DOMD (kg kg-1 DM) (MAFF 1984). The protein value of the samples is expressed as the amino acids absorbed from the small intestine (metabolizable protein, MP) and the protein balance in the rumen (PBV) according to Luke (2020). After harvesting the whole crop samples were frozen and during the winter 2018–2019 stem, leaf and ear frac- tions were separated manually. The fractions were dried at 60 °C for three days to determine the DM concentra- tion. The proportion of the fractions was measured on a DM basis. The ash concentration of the fractions was determined by ashing at 600 °C for 2 h. The starch concentration of the ears was analyzed according to Salo and Salmi (1968). The digestibility of the fractions was based on the in vitro pepsin-cellulase solubility (Nousiainen et al. 2003, Huhtanen et al. 2006). The sward density, which describes the coverage of the grass, was measured in spring when grass growth had be- gun. The density (%) was evaluated by using a scale 0 to 100 (0=totally destroyed, 100=fully covered). The temperature sum (degree days, DD +5 °C) and precipitation were measured during the growing season from May to September. A meteorological station of the Finnish Meteorological Institute, which automatically provides data of daily temperature and rainfall is located at the Research Station in Ruukki. K. Manni et al. 27 Statistical methods The normality of the analyzed variables was checked using graphical methods: these included box- and scat- ter-plots of the residuals and fitted values. The data were subjected to an analysis of variance using the SAS MIXED procedure (version 9.4, SAS Institute Inc., Cary, NC, USA). The data from each year were analyzed and present- ed separately. In 2017, the experimental design was a split-plot with four replicates with a randomized complete block arrangement of treatments. The main block consisted of the combined effect of reduced fertilizer rate and reduced seed rate and the effect of variety was treated as the sub-plot. Crop varieties were randomized in the main blocks. In 2017, only three replicates were used for the DM yield due to the outlier observations in one replicate. In 2017, the data were analyzed for a split-plot design using the statistical model y ijk = µ + ρ i + α j + w ij + β k + (α×β) jk + ε ijk where μ is the overall mean and α j, β k and (α×β) jk are the combined fixed effects of the fertilizer and seed rate, plant variety and their interaction, respectively, ρ i is the random effect of the block, w ij is the residual error term of the combined fertilizer and seed rate, and ε ijk is the residual error term of the plant variety. In 2018, the data were analyzed for a randomized complete block design using the statistical model y ij = µ + ρ i + τ j + + ε ij where μ is the overall mean and τ j is the fixed effect of the plant variety, ρ i is the random effect of the block, and ε ij is the residual error term of the plant variety and block. Differences between the treatments were tested using Tukey’s test. The results were considered statistically sig- nificant when p<0.05, which was used to determine significant differences between the means in the main ef- fects for the plant varieties, combined fertilizer and seed rate, and in interactions between plant varieties and combined fertilizer and seed rates. Results Weather conditions The temperature sum during the growing season in 2017 was slightly lower than average, whereas in 2018 it was considerably higher than average (Table 2). The precipitation sum from May to July in 2017 was close to the long term average, but in August it was considerably higher (Table 2). The precipitation during the growing season in 2018 was below average and July especially was dry. Yields of whole crops For the DM yield, no significant fertilizer and seed rate × variety interaction was observed in 2017 (Table 3). The treatment with reduced fertilizer and seed rate decreased (p<0.001) average whole crop yield by 13% (9539 vs. 8299 kg ha-1) . When comparing varieties across fertilizer and seed rates, the triticale variety Nagano yielded the highest (p<0.05) result. The DM yield between varieties varied from 8650 to 9427 kg ha-1. Table 2. Temperature sum (degree days, DD +5 ⁰C) and precipitation at the Research Station in Ruukki (64°44’N, 25°15’E) in 2017–2018 compared to the long term average during 1981–2010 (source: Finnish Meteorological Institute) Temperature sum (DD), ⁰C Precipitation, mm 2017 2018 1981–2010 2017 2018 1981–2010 May 41 211 95 37 23 42 June 213 216 244 41 45 50 July 310 466 338 77 25 77 August 265 322 263 122 87 71 September 110 171 113 38 58 50 Total (May-September) 938 1386 1052 315 237 289 Agricultural and Food Science (2021) 30: 24–35 28 In 2018, the DM yield between varieties varied from 7043 to 10622 kg ha-1 (Table 4). Among the species, triticale variety Bikini had lower (p<0.05) DM yield than that of other triticale varieties. Differences between barley vari- eties were not found. Compared to the barley and wheat varieties, the triticale variety Somtri had higher (p<0.05) DM yield than the barley varieties Kaarle and Armas and the wheat variety Helmi. The triticale variety Bikini had lower (p<0.05) DM yield than the barley variety Trekker. Chemical composition and feed values The chemical composition and feed values of the whole crops in 2017 are presented in Table 3. Except for the WSC concentration, no significant fertilizer and seed rate × variety interactions were observed. There were significant differences between varieties in all chemical composition parameters and feed values. The wheat variety Helmi had a 7% higher (p<0.05) CP concentration than that of the barley variety Kaarle. The treatment with reduced fertilizer and seed rate decreased (p<0.05) the CP concentration by 8% (87 vs. 81 g kg-1 DM). The highest (p<0.05) NDF and iNDF concentrations were for the wheat variety Helmi and both triticale varieties Nilex and Nagano. The barley varieties Trekker and Kaarle had the lowest (p<0.05) NDF concentrations and the Trekker variety had the lowest (p<0.05) iNDF concentration among all the five varieties. For the WSC some significant fertilizer and seed rate × variety interactions (p=0.008) were observed (Table 3, Fig. 1). For the triticale variety Nagano the WSC concentration decreased when the fertilizer and seed rate was reduced while for the other varieties the effect was contrary. Both barley varieties Trekker and Kaarle had higher (p<0.05) D-values and ME concentrations than the others, Trekker having the highest values. Numerically the differences in ash, MP and PBV concentrations were minor although some significant differences were observed between the varieties. 1 The reduced fertilizer and seed rate was 30% of the normal level; 2 Standard error of mean; 3 Means in the same row with different superscript letters are significantly different (p < 0.05); 4 Interaction (whether amount of fertilization and seed rate has a different effect with varieties) Table 3. Yield, chemical composition, and feed values of whole crops at normal or reduced (30% of the normal level) fertilizer and seed rate and for different varieties of barley, triticale and wheat harvested at the early dough stage and in 2017 Fertilizer and seed rate1 Triticale Barley Wheat p-values3 Normal Reduced Nagano Nilex Kaarle Trekker Helmi SEM2 Fertilizer and seed rate Variety Interaction4 Yield, kg dry matter (DM) ha-1 9539a 8299b 9427a 8808b 8927b 8650b 8785b 102.7 <0.001 <0.001 0.119 Chemical composition, g kg-1 DM Crude protein 87a 81b 85ab 84ab 81b 83ab 87a 1.6 0.023 0.049 0.279 Neutral detergent fibre (NDF) 461 458 473a 477a 441b 419b 487a 7.3 0.551 <.0001 0.931 Indigestible NDF 150 149 161a 160a 141b 118c 166a 4.2 0.633 <.0001 0.728 Water soluble carbohydrates 154 164 198 212 113 115 157 2.7 <0.001 <.0001 0.008 Ash 46 45 47ab 46bc 44cd 42d 49a 1.0 0.085 <.0001 0.104 Digestible organic matter 612 613 595c 598c 626b 654a 591c 4.8 0.764 <0.001 0.604 Feed values Metabolizable energy, MJ kg-1 DM 9.8 9.81 9.5c 9.6c 10.0b 10.5a 9.5c 0.08 0.821 <.0001 0.776 Metabolizable protein, g kg-1 DM 69 68 67c 67c 70b 72a 67c 0.5 0.269 <.0001 0.481 Protein balance in the rumen, g kg-1 DM -17a -23b -17a -17a -24b -27b -14a 1.3 0.023 <.0001 0.393 K. Manni et al. 29 Chemical composition and feed values of the whole crops in 2018 are presented in Table 4. The CP concentration of the wheat variety Helmi was higher (p<0.05) than that of all barley varieties, and higher than the triticale vari- eties Somtri and Nagano. Among the triticale varieties, the Somtri variety had a lower (p<0.05) CP concentration compared to the varieties Nilex and Bikini but it did not differ from the Nagano variety. The triticale variety Nilex had lower (p<0.05) NDF concentration compared to the triticale variety Somtri and the barley varieties Armas and Kaarle. The barley variety Trekker had lower (p<0.05) iNDF concentration compared to the barley variety Kaar- le and the triticale variety Somtri. Differences between varieties in terms of the D-value and ME concentrations were minor. The only significant difference (p<0.05) was between the barley varieties Trekker (higher) and Kaarle (lower) both in their D-values and in ME concentrations. Table 4. Dry matter yield, chemical composition, and feed values of different varieties of barley, triticale and wheat when harvested at the early dough stage in 2018 Triticale Barley Wheat SEM1 p-values2 Variety Bikini Nagano Nilex Somtri Armas Kaarle Trekker Helmi Yield, kg dry matter (DM) ha-1 7043c 9483ab 9240ab 10622a 7901bc 7838bc 9092ab 7650bc 405.9 <0.001 Chemical composition, g kg-1 DM Crude protein 100ab 90bc 99ab 85c 89bc 87c 94bc 106a 2.8 <0.001 Neutral detergent fibre (NDF) 471ab 468ab 442b 508a 501a 509a 465ab 469ab 12.4 0.003 Indigestible NDF (iNDF) 148ab 158ab 148ab 170a 153ab 167a 136b 155ab 6.8 0.010 Water soluble carbohydrates 156a 105ab 119ab 152a 72b 84b 106ab 93b 11.8 <0.001 Ash 50b 50b 51b 50b 60a 61a 55ab 55ab 1.9 0.001 Digestible organic matter 613ab 602ab 613ab 592ab 598ab 580b 625a 603ab 8.4 0.011 Feed values Metabolizable energy, MJ kg-1 DM 9.8 ab 9.6ab 9.8ab 9.5ab 9.6ab 9.3b 10.0a 9.7ab 0.136 0.011 Metabolizable protein, g kg-1 DM 71 ab 68abc 70ab 67bc 68abc 66c 71a 70ab 1.0 0.002 Protein balance in the rumen, g kg-1 DM -6 ab -13bc -7ab -16c -13bc -12bc -14bc 1a 2.0 <0.001 1 Standard error of mean; 2 Means in the same row with different superscript letters are significantly different (p < 0.05) Fig. 1. Differences between varieties in water soluble carbohydrates (g kg-1 dry matter), and the response to the amount of fertilizer and seed rate (normal vs. lower 30% of the normal level) when harvested at the early dough stage (interaction p= 0.008; amount of fertilizer and seeding rate × varieties). Different letters in the bars indicate significant differences (p < 0.05) between means. Agricultural and Food Science (2021) 30: 24–35 30 Fractions The proportion, composition, and digestibility of fractions in the whole crops are presented in Table 5. The pro- portion of ear in the DM varied from 456 to 672 g kg-1 DM between the varieties. Among the triticale varieties, Nagano and Nilex had higher (p<0.05) proportion of ear than that of Bikini and Somtri. Bikini variety had the low- est (p<0.05) proportion of ear compared to all other varieties. Among the barley varieties, Armas and Kaarle had higher (p<0.05) proportion of ear than Trekker. The proportion of stems in the DM varied from 267 to 461 g kg-1 DM between the varieties. The triticale variety Bikini had a higher (p<0.05) proportion than all other varieties. The proportion of leaves was clearly lower than for the ears or stems and it varied from 52 to 97 g kg-1 DM between the varieties. The ear:stem ratio varied from 1.00 to 2.53 between the varieties. The highest (p<0.05) ear:stem ratio was for the barley varieties Armas and Kaarle and the lowest (p<0.05) was for the triticale varieties Bikini and Somtri. The starch concentration in the ears varied from 365 to 538 g kg-1 DM between the varieties. The lowest (p<0.05) starch concentration in the ears was for the triticale variety Bikini. The highest (p<0.05) ash concentrations in the stems and leaves were found in the barley varieties, except in stems of Trekker. The pepsin-cellulase solubility of the ears varied from 915 to 974 g kg-1 organic matter (OM). The highest (p<0.05) solubility of the ears was found in the barley varieties Kaarle and Armas and the lowest (p<0.05) was found for the triticale variety Bikini. For the stems, the pepsin-cellulase solubility was contrary to the solubility found for the ears. The highest (p<0.05) solubility of the stems was found in the triticale variety Bikini and the bar- ley variety Trekker, and the lowest (p<0.05) was determined for the barley varieties Kaarle and Armas. Between the varieties, the solubility of the stems varied from 455 to 645 g kg-1 OM. For the leaves, the pepsin-cellulase solubility varied from 786 to 848 g kg-1 OM. Triticale variety Bikini had higher (p<0.05) solubility of the leaves than that of triticale variety Nagano, barley varieties Armas and Kaarle and wheat variety Helmi. Barley varieties Armas and Kaarle and wheat variety Helmi as for had lower (p<0.05) solubility of the leaves than that of all other varie- ties, except for triticale variety Nagano that did not differ from them. Table 5. Proportion, composition, and digestibility of fractions for varieties of barley, triticale and wheat when harvested at the early dough stage in 2018. Triticale Barley Wheat SEM1 p-values2 Variety Bikini Nagano Nilex Somtri Armas Kaarle Trekker Helmi Fractions3 Proportion of ear, g kg-1 DM 456e 641abc 597c 519d 672a 662ab 611c 623bc 9.9 <0.001 Proportion of stem, g kg-1 DM 461a 307cde 338c 401b 267e 269e 292de 309cd 8.6 <0.001 Proportion of leaves, g kg-1 DM 82ab 52e 65cde 79bc 61de 70bcd 97a 68bcd 3.3 <0.001 Ear:stem ratio 1.00d 2.09b 1.77c 1.30d 2.53a 2.47a 2.09bc 2.02bc 0.067 <0.001 Ear Pepsin-cellulase solubility, g kg-1 organic matter 915 d 947b 949b 936c 968a 974a 953b 950b 3.0 <0.001 Starch, g kg-1 DM 365e 522ab 538a 468d 483cd 505bc 487cd 499bc 6.2 <0.001 Ash, g kg-1 DM 36a 29de 28e 33b 31bcd 30cde 32bc 28de 0.63 <0.001 Stem Pepsin-cellulase solubility, g kg-1 organic matter 645 a 539c 580b 580b 456d 455d 621a 512c 6.4 <0.001 Ash, g kg-1 DM 43cd 51bc 51bc 40d 70a 68a 56b 53b 2.2 <0.001 Leaves Pepsin-cellulase solubility, g kg-1 organic matter 848 a 817bc 843ab 839ab 786c 792c 842ab 801c 6.6 <0.001 Ash, g kg-1 DM 83c 100b 97b 82c 119a 127a 128a 90bc 2.5 <0.001 1 Standard error of mean; 2 Means in the same row with different superscript letters are significantly different (p < 0.05); 3 Reported SEM is calculated for 4 replicates. In Trekker the number of replicates was 3 and SEM must be multiplied by 1.2. K. Manni et al. 31 Effects of fertilizer and seed rate on the yield of the ley In 2017 when the ley was established, the cereal crops did not lodge. The treatment with reduced fertilizer and seed rate increased (p<0.05) the DM yield of the first cut and decreased (p<0.05) the yield of the regrowth but it did not affect the total grass DM yield in the first grass production year (Table 6). The DM yield with normal fer- tilizer and seed rates was on average 7766 kg ha-1 and at a reduced fertilizer and seed rate it was 7997 kg ha-1. Differences in the density of the ley sward in the spring were not found between the varieties or different ferti- lizer and seed rates (Table 6). The density of the ley sward in spring varied from 91 to 97% at the normal fertilizer and seed rates, and from 94 to 97% at the reduced fertilizer and seed rates. Calculation of whole crop silage production costs The calculated production costs varied between 0.16–0.22 EUR kg-1 DM, when the crop yields varied between 6500–10000 kg DM ha-1 (Appendix). If mean yield levels were used, the costs of different type of silages did not differ considerably, being 0.18–0.19 EUR kg-1 DM. Harvest and fertilizer costs were nearly double for clover-timothy silage compared to whole crop silage. The seed, tillage, and sowing costs were higher for the whole crop silage, because the costs occur annually for them. Discussion Yields of the whole crops The high DM yield potential of triticale is one reason that there is interest in cultivating it in northern regions. As was found in the experiment by Nadeau (2007), triticale and spring wheat had greater DM yields than oats and barley when harvested as a whole crop at the early dough stage of maturity. However, contrary to the hypothesis and results by Nadeau et al. (2007), in the present experiment there was no clear evidence that triticale varieties produced higher whole crop yields compared to barley and wheat varieties. Based on results from one year, the Bikini variety produced lower DM yields than other triticale varieties. However, due to the rather extensive dis- persion in the data in 2018, it makes difficult to interpret the results. There are also experiments where the DM yield of spring triticale has been lower than other crops (Berkenkamp and Meeres 1987, Khorasani et al. 1997). The differences between the studies may be related to several factors, e.g. weather conditions, soil type and the varieties studied. Chemical composition, feed values and fractions It is well established that increased N fertilizer increases the CP concentration of whole crops (Cazzato et al. 2012, Li et al. 2016), which is in line with the present experiment in 2017. However, in both years the CP concentration was rather low in all crop varieties and the differences between them were minor. Contrary to Nadeau (2007), Table 6. Dry matter (DM) yield of two cuts and density in spring of a ley stand in the first production year 2018. The ley was established in 2017 with different varieties of triticale, barley, and wheat at normal or reduced (30% of the normal level) fertilizer and seeding rates and the crops were harvested as whole crops. Fertilizer and seed rate Triticale Barley Wheat SEM1 p-values2 Normal Reduced Nagano Nilex Kaarle Trekker Helmi Fertilizer and seed rate Variety Interaction3 DM yield, kg ha-1 Primary growth 4403 4833 4806a 4839a 4156b 4682ab 4604ab 136.7 0.001 0.010 0.620 Regrowth 3363 3164 3260 3112 3428 3190 3329 150.5 0.043 0.275 0.648 Total 7766 7997 7808 7882 7592 7810 7737 293.1 0.156 0.406 0.824 Density of ley stand in spring, %4 94 96 97 95 91 93 93 2.4 0.457 0.238 0.826 1 Standard error of mean; 2 Means in the same row with different superscript letters are significantly different (p < 0.05); 3 Interaction (whether amount of fertilization and seed rate has a different effect with varieties); 4 Scale 0 to 100 (0=totally destroyed, 100=fully covered) Agricultural and Food Science (2021) 30: 24–35 32 there was no evidence in the present experiment that triticale has higher CP concentration than barley or wheat. Khorasani et al. (1997) found that at the boot stage, triticale and barley had higher CP concentrations than oats, but as maturity advanced, the CP concentration decreased in all species, and when harvested at the soft dough stage there were no differences between the cereals. For whole crop silage it is typical that the CP concentration is not very high and mostly it is lower than moderately digestible grass silage (Huhtanen et al. 2006, Huuskonen and Joki-Tokola 2010, O´Kiely 2011, Huuskonen et al. 2020). In the whole data of the present experiment the CP concentration varied from 81 g kg-1 DM to 106 g kg-1 DM. In our data from 2017, the highest NDF concentration was found in the wheat and triticale varieties. This result is in line with the experiments by Huuskonen et al. (2020) and Khorasani (1997), in which the NDF concentration of triticale was found to be higher compared to barley when whole crops were harvested at the soft dough stage. Nadeau (2007) reported that triticale had the lowest NDF concentration whereas oats and wheat had the highest when harvested at the early dough stage. In our data from 2018 no clear differences in the NDF concentration be- tween the species were found, which indicates the variation between the years. Typically, the NDF concentration is affected by stage of maturity and the ear:stem ratio as the cell walls are primarily in the plant stem (Khorasani 1997, Nadeau 2007, Wallsten et al. 2010). However, when observing data from 2018, there was a clear differences in ear:stem ratio but not in NDF concentration between the varieties. In the present experiment, the triticale variety Bikini had a high WSC concentration, low starch concentration in the ears and a low ear:stem ratio. This may indicate that the Bikini variety was harvested too early in the growth stage, although all varieties were assessed to be at the soft dough stage when harvested by following the ker- nel development according to the Zadoks growth scale (Zadoks et al. 1974). If the Bikini variety was harvested at too early of a stage, it also may have contributed to the low DM yield. According to Givens et al. (1993), the as- sessment of the growth stage alone may not be a good guide to the composition and digestibility of whole crop cereal silage.They concluded that it is a subjective technique and in addition, other factors, such as variety and agronomic practises, particularly cutting height, may also have an influence on the composition and digestibility. There were significant differences in the fractions of stems, leaves and ears between the crop varieties. For all va- rieties, the proportion of leaves was substantially lower than the proportion of ears and stems. Then although the leaves had high digestibility, the effects on the chemical composition and nutritive values of the whole biomass was rather small. Except for the triticale variety Nagano, triticale had a higher proportion of stems compared to barley, which is similarly to the findings by Khorasani et al. (1997). In 2017 both barley varieties had higher D-values than the triticale and wheat varieties, and this result is in line with previous experiments by Huuskonen et al. (2020). Also, Cherney and Marten (1982) reported higher in vitro digestibility in barley compared to oats, wheat and triticale. Similarly, Nadeau (2007) observed that barley but also triticale had higher in vitro digestibility than oats and wheat. However, in 2018 a similar difference was not found. Previously, it has been found that the growth stage and in consequence, the ear:stem ratio are factors that mostly affect the chemical composition and digestibility of whole crop silage (Khorasani et al. 1997, Nadeau 2007, Rustas 2009). However, in the present experiment, the ear:stem ratio did not clearly affect the digestibility of the crops as differences in the digestibility of the fractions compensated for the differences in the ear:stem ratio. This result is in line with a previous study by Sinclair et al. (2003) in which increasing the cutting height of whole crop wheat at harvest did not affect the apparent digestibility determined by using sheep. Although in the present experiment there were differences in the ear:stem ratio, only the barley varieties Kaarle and Trekker differed in terms of their digestibility: the Trekker variety had a higher D-value even though the Kaarle variety had a higher ear:stem ratio. The result is probably due to clearly higher solubility of the stems in the Trekker variety. However, the barley varieties Armas and Kaarle, which had the highest ear:stem ratio, also had the highest pepsin-cellu- lase solubility for the ears but the lowest solubility for the stems and leaves. The lowest ear:stem ratio was found for the triticale varieties Bikini and Somtri, and they also had the lowest pepsin-cellulase solubility for the ears. However, a higher proportion of stems and particularly higher digestibility of the stem and leaves compensated for the lower digestibility of the ears and they did not differ from the others when assessed by the D-value. The results of the present experiment support the conclusion by Sinclair et al. (2003) that showed that the propor- tion of stems is not a major factor limiting the ME value of the forage which actually results from the digestibility. Effects of fertilizer and seed rate on the yield of the ley It was hypothesized that reduced seed and fertilization rates could prevent the lodging of the cereals used as nurse crops for the undersown new ley and subsequently promote grass growth. Because the cereal crops did K. Manni et al. 33 not lodge, the hypothesis could not be evaluated. However, theoretically this method could be used as an insur- ance against lodging and the cost is about 1175 kg DM ha-1 in terms of the reduced cereal crop yield. However, when assessing the costs, it also should be taken into account that production cost was reduced, due to less fer- tilizer and seed. In the first production year, the total DM yield and density of the ley sward in the spring were good and the treatment with reduced fertilizer and seed rate did not affect them. The crop varieties did also not affect the growth of undersown ley. Calculation of whole crop silage production costs The differences in the production costs of different silage types were lower than expected. Whole crop silage has to be established annually, while grass ley can be assumed to be established every third year in northern condi- tions. Seed, fertilizer, and harvest costs differ greatly between silage types. The costs contradicted each other so that finally the production costs did not differ to a great extent, although the calculation is sensitive to yield as- sumptions. For example, if the yield of grass ley rose to 10000 kg DM ha-1 and the other silage types remained at the mid-level, the production costs of grass silage would be clearly the cheapest (0.15 EUR kg-1 DM). In this cal- culation DM yields did not represent the final silage DM yields because they did not include harvest, storage and ensiling losses. However, these losses may be substantial and different depending on harvest method and ensiling process. They also may vary widely that makes it difficult to assess the amount of losses in the calculation. The calculation mainly indicates that when using average assumptions, the annual whole crop silages are not signifi- cantly more expensive to produce than perennial grass silage. When comparing production costs of different silage types, it is essential to notice that these silage types have very different values in terms of animal feeding. For example, barley whole crop silage has a different value for dairy feeding than high quality grass silage. Whole crop cereal silage can play an important role as a component in mixed ration feeding. The production costs of whole crop silage types can be compared also to commercial feed prices. In this case, sub- sidies based on field areas should be taken into account. These subsidies would reduce the prices of silage con- siderably in our production costs calculation. Conclusions Varieties of triticale can produce high DM yields and it does not differ substantially in nutritive value from barley and wheat. All the studied species and varieties in this research were suitable raw materials for whole crop silage. However, due to the variation between the years, more experiments are needed to find generally applicable dif- ferences between the varieties. High DM yield of the ley in the first production year was possible to achieve us- ing small grain cereals as a nurse crops and harvest them as a whole crop. Under the current circumstances, the reduced cereal seed and fertilization rates did not affect the yield of the undersown ley. The production costs of the different whole crop silage types were surprisingly similar. Acknowledgements This study was partially funded by the Centre for Economic Development, Transport and the Environment for North- ern Ostrobothnia, Oulu, Finland, Eastman Chemical Company, Berner Ltd and Hankkija Ltd. 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Fertilizer N kg ha-1 Costs EUR kg-1 Amount kg ha-1 Faba bean + wheat silage EUR ha-1 Barley- silage EUR ha-1 Triticale- silage EUR ha-1 Clover + timothy silage EUR ha-1 Ploughing, seedbed tillage, sowing 202 202 202 0 Seed costs Faba bean 0.88 250 220 Wheat 0.49 100 49 Barley 0.45 200 90 Triticale 1.05 250 263 Clover + timothy 4.70 8 38 Fertilizer, NPK (22-5-5) Faba bean + wheat 50 0.40 227 91 Barley 90 0.40 409 164 Triticale 90 0.40 409 164 Clover + timothy 180 0.40 818 327 Silage additive + silage film 58 58 58 58 Harvest to the clamp silo Faba bean: mowing + pick up harvester 200 Barley and triticale: direct cut harvester 220 220 Grass ley: mowing + pick up harvester x 2 400 Interest of working capital (50%, 5%) 21 18 23 11 Costs of clamp silo 219 219 219 219 Costs of field 416 416 416 416 Overhead costs 66 66 66 66 Total costs 1542 1453 1630 1535 Estimated yield range, kg DM ha-1 7000–9000 6500–8500 8000–10000 7000–9000 Costs, EUR kg-1 DM 0.22–0.17 0.22–0.17 0.20–0.16 0.22–0.17 Mean yield, kg DM ha-1 8000 7500 9000 8000 Costs, EUR kg-1 DM 0.19 0.19 0.18 0.19 Untitled Comparing spring triticale varieties to barley and wheat varieties Introduction Material and methods Plant material and experimental design Feed analyses and measurements Statistical methods Results Weather conditions Yields of whole crops Chemical composition and feed values Fractions Effects of fertilizer and seed rate on the yield of the ley Calculation of whole crop silage production costs Discussion Yields of the whole crops Chemical composition, feed values and fractions Effects of fertilizer and seed rate on the yield of the ley Calculation of whole crop silage production costs Conclusions Acknowledgements References Appendix