A G R I C U LT U R A L A N D F O O D S C I E N C E A. M. García-López et al. (2015) 24: 249–260 249 Manuscript received March 2015 Plant uptake of phosphorus from sparingly available P- sources as affected by Trichoderma asperellum T34 Ana M. García-López, Manuel Avilés and Antonio Delgado Agroforestry Sciencie Department University of Seville. Ctra Utrera Km 1. 41013 Seville, Spain e-mail: adelgado@us.es The contribution of Trichoderma asperellum T34 to the plant uptake of phosphorus (P) from sparingly phytoavailable forms such as insoluble calcium (Ca) phosphates and phytates was studied. Two experiments with cucumber (Cucumis sativus L.) on siliceous sand were performed involving two factors, namely: (i) P source, viz. KH 2 PO 4 , phytate (Ins6P), and phos- phate rock (PR), and (ii) inoculation with T34. Liquid pure cultures of T34 were also used. T34 increased the total content in P of cucumber roots irrespective of the particular P form and enhanced total P uptake by plants with P supplied as Ins6P or PR. The increased phytase activity observed with T34 contributes to explain its favourable influence on the uptake of P supplied as Ins6P. Solubilization of Ca phosphates from PR was favoured by the slightly acidifying effect and the increased organic anion concentration promoted by the fungus in the plant growth media. It can be concluded that T34 can improve P nutrition in plants grown on media containing phytates or insoluble Ca phosphates as dominant P forms. Key words: phosphorus, Trichoderma, rhizosphere, Ca phosphates, phytase. Introduction The consequences of the depletion of P resources have aroused increasing interest in recent years, especially af- ter the rapid increase of phosphate rock prices in 2007 and 2008 (Cordell et al. 2009, Gilbert 2009). Efficient use of P can help to make agriculture sustainable in a scenario of increasing prices of fertilizers owing to the rapid de- pletion of available reserves (Cordell et al. 2009, Ryan et al. 2012). Also, unlocking residual P forms bound to soil is an essential stage towards a sustainable future for agriculture (Stutter et al. 2012). Soil microorganisms can facilitate the mobilization of soil P through various mechanisms (Wang et al. 2012). Many rhizobacteria and fungi possess mechanisms to mobilize Fe, and hence Fe-bound P, including chelator release, acidification (Marschner et al. 2004, Lemanceau et al. 2009) and reduction processes (Sánchez-Alcalá et al. 2011). Acidification by plant growth-promoting bacteria facilitates uptake of P from poorly soluble —and hence largely unavailable— Ca phosphates by plants (Browne et al. 2009, Singh and Reddy 2011). Also, rhizosphere microflora is known to promote the hydrolysis of organic P forms by releasing hydrolytic enzymes, which facilitates their use as P sources by plants (Hariprasad and Niranjana 2009, Patel et al. 2010). The mobilization of P from poorly solu- ble Ca phosphates and stable organic forms such as phytates can be important in many environments, where both sparingly available P forms frequently account for most P present in soil (Celi et al. 2001, Saavedra et al. 2007). In- creasing the acquisition of P by plants from these residual soil P forms by microbial inoculation can contribute to a more sustainable management of non-renewable P resources and to improve the productivity of P-poor soils, which expand on one third of the agricultural land in the World (MacDonald et al. 2011). Biological control agents can be effective in mobilising P in the rhizosphere, which warrants their use for integrated management of plant diseases and P supply to plants (Vassilev et al. 2006), thus contributing to a more sustainable agricultural production. The soil fungi Trichoderma spp. are efficient, widely used biological control agent (Howell 2003, Schuster and Schmoll 2010) that enhances nutrient uptake by plants (Yadav et al. 2009, de Santiago et al. 2013). In spite of the effects described by Altomare et al. (1999) or Rudresh et al. (2005), little information about contribution of Trichoderma to P uptake by plants is available. Although the positive contribution of P-mobilizing microorganisms to plant nutrition is well-known, evidences of mobilization from specific P forms and mechanisms involved in this process are most often limited to pure cultures of microorganisms and rarely observed in plant growth studies (Singh and Reddy 2011, Wang et al. 2012). For accurate assessment of their use to improve plant nutrition, it is required: (i) more direct evidences of the effect of microorganisms on plant P uptake from specific P forms present in soil, (ii) a deeper knowledge about mechanisms involved in P mobilization in plant growth me- dia, and (iii) how these mechanisms interact with P-mobilizing mechanisms of plants. A G R I C U LT U R A L A N D F O O D S C I E N C E A. M. García-López et al. (2015) 24: 249–260 250 This work was undertaken in order to study the contribution of Trichoderma asperellum T34, an effective bio- control agent (Segarra et al. 2007), to plant uptake of P from sparingly phytoavailable forms such as insoluble Ca phosphates and phytates by plants, and also to elucidate the mechanisms behind this potential effect in plant growth media with a view to facilitating the integrated use of the fungus in biocontrol and fertilization strategies. Materials and methods Experimental setup Two sets of experiments were performed, namely: (i) cultivation of T34 in liquid media, in order to assess its P- mobilizing mechanisms and (ii) inoculation experiments with plants grown in artificial growth media to assess how T34 can contribute to P nutrition of plants from different P sources. Cultivation of T34 in liquid media Trichoderma asperellum T34 was cultivated in Czapek liquid media containing P from various sources in order to examine the potential enzyme production and acidification abilities of the fungus. The media contained sucrose (30 g L–1), NaNO 3 (2 g L–1), MgSO 4 (0.1 g L–1), KCl (0.5 g L–1) and FeSO 4 ·7H 2 O (0.01 g L–1), and had a pH of 7.8. A com- pletely randomized experiment with 3 replications was performed by using an identical T34 inoculation rate (100 conidia mL–1) and a single factor (P source) with 4 different treatments, namely: (i) phosphate applied as KH 2 PO 4 at full strength of 0.23 g L –1 P, which is the usual concentration for this type of medium, (ii) KH 2 PO 4 at 23 mg L –1 P (1/10 strength KH 2 PO 4 ), (iii) ground (< 0.1 mm) phosphate rock (PR, essentially apatite) at 0.23 g L –1 P, and (iv) phytate (myo-inositol-6-hexakisphosphate Na salt, Ins6P) at 0.23 g L –1 P. The low KH 2 PO 4 treatment (23 mg L –1 P) was included to study responses of T34 to suboptimal phosphate concentrations in solution that were greater than that expectable with an insoluble P source (RP). Cultivation runs were done in 250 mL glass flasks. After 7 days of cultivation, the media were passed through a cellulose nitrate filter of 0.22 µm pore size and used to determine fungal biomass, enzyme activities in solution, pH, molybdate reactive P (MRP) according to Murphy and Riley (1962), and the concentration of major organic anions produced (particularly those capable of mobiliz- ing Fe and P such as oxalic and citric). Inoculation experiments with plants Experiments with cucumber plants (Cucumis sativus L. cv Tropico) were performed by using an artificial growth medium consisting of siliceous sand previously sieved through 0.5 and 1 mm mesh in order to ensure adequate aeration and hydraulic conductivity. The sand was washed several times with 0.2 M Na 2 CO 3 to improve dispersion and remove impurities. The source of Fe in the growth media was ferrihydrite, which was supplied by coating part of the siliceous sand with this oxide according to Rahmatullah and Torrent (2000). The concentration of Fe bound to ferrihydrite in the growth media, estimated as citrate-ascorbate extractable Fe, was 200 mg kg–1 Fe medium. The pH of the media was 8 and the electrical conductivity (EC) in all (1:5 medium:water extract) lower than 100 μS m–1. Two different experiments were conducted under exactly the same plant growth conditions by using a completely randomized design with five replications (one pot per replication and one plant per pot) and two factors, namely: P source, with three different treatments (KH 2 PO 4 , Ins6P and PR [<0.1] mm), and T34 (tests with and without in- oculation [control]). Phosphorus from KH 2 PO 4 and Ins6P was supplied in the form of compounds adsorbed on Fe oxides covering part of the siliceous sand in order to imitate the major fractions of these P forms in soils which are usually bound to Fe oxides as dominant sorbent surfaces, whereas PR was carefully mixed with the growth media before trans- planting. This growth medium was intended to mimic the dynamics of two major soil P forms not readily avail- able, namely: sparingly soluble Ca phosphates and phytates (Saavedra and Delgado 2005). The P rate used in all the cases was 6.4 mg of P per kg of growth medium, equivalent to 2.56 mg pot–1 P (viz., 2–3 times the amount of P expected to be absorbed by each plant). Phosphate and Ins6P were adsorbed on siliceous sand coated with Fe oxides by immersing 1 kg of sand in 1.5 L of the same nutrient solution used in the experiment, which contained 5.25 and 6 mg L–1 P as KH 2 PO 4 and Ins6P sodium salt, respectively. Phosphorus concentration in surrounding A G R I C U LT U R A L A N D F O O D S C I E N C E A. M. García-López et al. (2015) 24: 249–260 251 solution was determined daily and nutrient solution with P replaced by a new one with the same P forms and concentration until the desired adsorbed P amount was obtained. After adsorption, P concentration in equilib- rium with siliceous sand coated with Fe oxide was 1.5 mg L–1 P and 3 µg L–1 P for KH 2 PO 4 and Ins6P, respectively. Then, to check that amounts of sorbed P were those estimated, and to know the dominant forms in which P was retained in the medium, a sequential extraction with 0.1M NaOH + 1 M NaCl and 1 M HCl was performed. The first extraction step, which was intended to desorb P from Fe oxides, recovered 53% of inorganic phosphate and 45% of Ins6P, the second step released the remaining sorbed P —mostly precipitated P, which indicates that a substantial fraction of the inorganic phosphate or InsP6 supplied precipitated as Ca salts (Ca provided by nutrient solution) as it can be expected in soils with similar pH (e.g. Lindsay (1979) for inorganic phosphate, or Celi et al. (2001) for InsP6). Phosphorus in nutrient solution used to adsorb P on Fe oxides and in the extracts of sequential extraction were determined colorimetrically (Murphy and Riley 1962), following persulphate digestion (Díaz-Es- pejo et al. 1999) in the case of those samples treated with Ins6P. Inoculation with T34 was carried out by immersing roots in a water suspension containing 103 conidia mL–1 be- fore transplanting (de Santiago et al. 2009), followed by addition of 104 conidia g–1 medium after transplanting. The microorganisms were supplied in 20 mL of a water suspension containing 2·105 conidia mL–1 and prepared according to Segarra et al. (2007), a micropipette was used to apply the conidia at four points around each plant. Seeds were sown in 35 mL polyvinyl multipots that were filled with perlite and irrigated with de-ionized water. After 28 days, plants were transplanted in the growth medium and allowed to grow for a further 26 days. Each pot (350 mL, 5.5 cm diameter, 15 cm high polystyrene cylinder) was filled with 400 g of siliceous sand (80 g with adsorbed Fe oxides) and used to grow a single plant. The experiment was conducted in a growth chamber with a photoperiod of 14 h, a 25/23 oC day/night temperature, 65% RH and 22 w m–2 light intensity. Pots were irrigated daily with of a Hoagland type nutrient solution without P and Fe. The total volume of solution applied per pot was 300 mL, and composition of the nutrient solution was (all concentrations in mmol L–1): MgSO 4 (2), Ca(NO 3 ) 2 (5), KNO 3 (5), KCl (0.05), H 3 BO 3 (0.024), MnCl 2 (0.0023), CuSO 4 (0.0005), ZnSO 4 (0.002) and H 2 MoO 4 (0.0005). The pH of the nutrient solution was 6. After the experiments, plant dry matter (DM) in shoots and roots was measured, and P and Fe concentrations in plant shoots and roots were determined. Rhizospheric growth medium, sampled as the medium retained by roots after hand-shaken them on a table during 30 s, was used to determine alkaline phosphomonoesterase, acid and alkaline phytase, T34 density and the concentration of low-molecular weight organic anions. Enzyme activity determination Phosphomonoesterase activity was determined as the amount of p-nitrophenol released from 5 mM p-nitrophe- nyl phosphate according to Tabatabai and Bremner (1969). Acid and alkaline phosphomonoesterase were deter- mined at pH 5.5 and 9, respectively, in the Czapek media used for liquid cultivation of T34, and at pH 9 in the plant growth media. Phytase activity in the plant growth media and in the filtered Czapek media was determined by incubating the media in MES buffer at a volume ratio of 1:1 at 37 °C for 60 min. The final concentrations in the as- say were 15 mM MES and 2 mM Ins6P —the latter was added as a substrate for the enzyme. The pH was 5.5 and 7.5 for acid and alkaline phytase, respectively. All solutions used were previously passed through a cellulose nitrate filter of 0.22 µm pore size for sterilization. After incubation, the reaction was stopped by adding an identical vol- ume of 10% trichloroacetic acid, and the suspensions centrifuged at 10 000 g for 10 min. The supernatants were used to determine molybdate reactive P according to Murphy and Riley (1962), phytase activity was expressed in enzyme units (1 µmol of molybdate reactive P released per min) per kg of growth medium. P sorption on Fe oxides present in the plant growing media during hydrolysis of Ins6P was assessed by incubation in a KH 2 PO 4 so- lution containing 4.2 mg P L–1 in order to measure the fraction of phosphate recovered after the phytase activity assay (George et al. 2005). P concentrations after incubation of the growth media with Ins6P were corrected for the ratio of P recovery in the media. A G R I C U LT U R A L A N D F O O D S C I E N C E A. M. García-López et al. (2015) 24: 249–260 252 Organic anion determination Organic anions were extracted from the plant growth media (Radersma and Grierson 2004) by using water at a 1:1 ratio with the sand and shaking in a end-over-end shaker at 4 Hz for 1.5 h. Then, the suspensions were cen- trifuged at 10 000 g for 10 min and the supernatants passed through filters of 0.45 μm pore size. With the Cza- pek media, the filtrates obtained from the 0.22 μm filters were directly used for analysis. Organic anions were separated by high performance liquid chromatography on an HPLC Varian ProStar 410 instrument equipped with a C18 column from Varian (250 mm × 34.6 mm, 8 µm particle size) at 45 °C, using isocratic elution with 98% 25 mM KH 2 PO 4 at pH 2 and 2% methanol as carrier at a flow rate of 0.8 mL min−1 (injected volume = 20 μL). Organic anions were detected at 215 nm, using a Varian 486 335 photo-diode array detector. Individual stock standard solutions (acetic, oxalic, citric, malic, fumaric and succinic) were prepared from Sigma chemicals and combined to obtain a dilute working-strength standard. Plant analysis Plant shoots and roots were separated and their dry mass (DM) was determined after drying to constant weight in a forced-air oven at 65 oC. Dried plant material was ground to pass through a 1 mm sieve prior to mineraliza- tion. An aliquot of 0.25 g was mineralized in porcelain crucibles in a furnace at 550 oC for 8 h. Then, 10 mL of 1 M HCl was used to dissolve ash and heated at 100 oC for 15 min, the digest being used to determine P and Fe. Phos- phorus was determined according to Murphy and Riley (1962) and Fe by atomic absorption spectrometry. Certi- fied plant material was also analysed in parallel to check for complete recovery of nutrients with this procedure. Seeds were also analysed with the same method in order to determine nutrient concentrations. Total uptake by plants was calculated as the total amount present in aerial parts and roots minus the total amount in seeds (Del- gado and Torrent 1997). Determination of T34 density in growing media The density of T34 in the rhizospheric media after the experiments was determined by dilution plating, using the Trichoderma semi-selective medium of Chung and Hoitink (1990) according to Borrero et al. (2012). To this end, 5 g of growth medium was suspended in 90 mL of a 1 g L–1 pyrophosphate solution in water. The suspension was shaken and a 10-fold dilution series from 10–1 to 10–4 was prepared with water agar (1 g L–1). Then, the suspen- sions were pipetted onto three plates per dilution. Colony forming units (CFU) were counted 7 days after plating and expressed as CFU mL–1 growth medium. No CFU was detected in the treatments without T34 inoculation. Statistical analysis An analysis of variance was performed by using the General Linear Model procedure in Statgraphics Plus 5.1 (Stat- Point, 2000) to identify the effects of the studied factors on the different variables measured in the experiments. Previously, data were checked for normal distribution and homoscedasticity by using the Kolmogorov–Smirnov test and Levenne test, respectively, as implemented with the previous software. Any data not passing either test required additional transformation (transformed data = data–b, being b the slope of the relation between the log- arithms of standard deviation and the logarithm of the mean for each treatment), which was effective to achieve ANOVA requirements. Each replication of the experiment with plants was considered a separate block in order to exclude the variation associated with repetition of the experiment (de Santiago et al. 2009). Only one factor (P source) was used in the analysis of variance of T34 CFU in the growing media because no CFU was detected in non-inoculated media. Means were compared via Tukey’s test; by exception, when interactions between factors were significant, the main effects could not be assessed in a combined analysis, so it was impossible to compare factor means between treatments (de Santiago et al. 2013). A mean comparison for the combined effect of fac- tors was performed when interaction was significant. Regressions and comparison between linear regressions were done by using the same model and software. A G R I C U LT U R A L A N D F O O D S C I E N C E A. M. García-López et al. (2015) 24: 249–260 253 Results Cultivation of T34 in liquid media Development of Trichoderma asperellum T34 in the liquid media, measured as the amount of biomass produced during incubation, differed depending on the P source; thus, it was higher with InsP6 and the more dilute KH 2 PO 4 than with the other P sources (Table 1). Significant phosphomonoesterase and phytase activities were observed in all the liquid media, the 1/10 KH 2 PO 4 , PR and Ins6P treatments exhibiting the highest phosphomonoesterase activity per gram of fungal biomass (Table 1). By contrast, phytase activity peaked with the full strenght KH 2 PO 4 concentration but was not significantly different from that observed with InsP6 (Table 1). No significant differences in low-molecular weight organic anions, mostly oxalate (>99% of anions detected; beside oxalate, only trace amounts of citric acid were observed; results not shown) —between P sources were observed (Table 1). In all cas- es, pH was decreased relative to its initial value in the liquid culture media, and significant differences between PR and Ins6P were observed (Table 1). MRP concentrations decreased in the following sequence: KH 2 PO 4 (0.23 g L–1) > Ins6P > 1/10 KH 2 PO 4 (23 mg L–1) > PR (Table 1). Experiments with plants Plant DM was significantly lower with PR than with the other two sources (p < 0.001; Tables 2 and 3). The P con- centration of shoots was lower with InsP6 (p < 0.01); however, the total P content of roots with this P source was not significantly different from that obtained with KH 2 PO 4 and PR (Table 3). Phosphorus source also affected the Fe concentration of roots and total Fe content in shoots and roots (Tables 2 and 3), Ins6P decreasing the Fe con- centration and total content of roots relative to KH 2 PO 4 (Table 3). Table 1. Effect of various P sources on Trichoderma asperellum T34 biomass, molybdate reactive P (MRP) concentration and pH, organic anions and enzyme production in liquid cultures of the fungus (Czapek media) Source of variation Phosphomonoesterase Phytase Biomass MRP pH Sum of organic anions Acid Alkaline Acid Alkaline P sourcea g L-1 mg L-1 mmol g–1 biomass mg pNP h-1 g–1 biomass EU g–1 biomass Phosphate rock 0.62 ± 0.02 c 3.1 ± 1.4 d 7.07 ± 0.16 a 37 ± 2 3.7 ±0.1 a 4.3 ± 0.8 a 0.7 ± 0.1 c 5.8 ± 1.1 c Ins6P 1.54 ± 0.1 a 159 ± 4 b 6.43 ± 0.1 b 36 ± 7 2.7 ± 0.5 a 3.3 ± 0.6 a 46.0 ± 7.8 a 54.2 ± 7.5 a 1/10 KH 2 PO 4 1.82 ± 0.34 a 17 ± 0.5 c 6.82 ± 0.22 ab 46 ± 8 2.7 ± 0.4 a 2.5 ± 0.6 a 3.3 ± 0.4 b 10.6 ± 1.2 b KH 2 PO 4 0.98 ± 0.1c b 227 ± 1 a 6.72 ± 0.06 ab 36 ± 7 1.3 ± 0.4 b 0.2 ± 0.1 b 66.7 ± 13.7 a 72.9 ± 15.3 a Means ± standard error, n = 3; a = all sources were supplied at a concentration of 0.23 g P L–1, by exception, 1/10 Phosphate was applied at 23 mg P L–1; Ins6P = myo-inositol-6-hexakisphosphate; pNP = p-nitrophenol released; EU = enzyme units (in μmol P min–1) released; MRP = molybdate reactive P as determined according to Murphy and Riley (1962); Means followed by different letters within a column are significantly different as per Tukey’s test at p < 0.05 for each factor A G R I C U LT U R A L A N D F O O D S C I E N C E A. M. García-López et al. (2015) 24: 249–260 254 Ta bl e 2. S ig ni fic an ce (p v al ue s) in th e an al ys is o f v ar ia nc e of th e di ff er en t v ar ia bl es s tu di ed in c uc um be r pl an ts (C uc um is s at iv us L .) an d th e pl an t g ro w th m ed ia Pl an ts G ro w th m ed ia So ur ce o f va ri at io n D ry m at te r P co nc en tr at io n To ta l P Fe co nc en tr at io n To ta l F e Ph os ph at as e Ph yt as e O xa la te pH CF U Sh oo ts Ro ot s To ta l Sh oo ts Ro ot s Sh oo ts Ro ot s up ta ke Sh oo ts Ro ot s Sh oo ts Ro ot s A lk al in e A ci d P so ur ce 0. 00 00 0. 00 13 0. 00 00 0. 00 21 0. 51 58 0. 00 00 0. 02 20 0. 00 01 0. 52 80 0. 02 58 0. 00 14 0. 00 70 0. 84 27 0. 00 00 0. 00 00 0. 00 08 0. 00 50 0. 25 49 T3 4 0. 00 76 0. 05 44 0. 00 87 0. 00 56 0. 69 74 0. 15 84 0. 02 10 0. 05 77 0. 60 21 0. 48 27 0. 00 63 0. 02 55 0. 89 22 0. 36 11 0. 01 47 0. 09 52 0. 03 92 P so ur ce × T 34 0. 29 91 0. 06 84 0. 19 07 0. 06 53 0. 61 61 0. 03 73 0. 38 71 0. 04 97 0. 73 83 0. 79 52 0. 31 13 0. 07 03 0. 03 40 0. 01 11 0. 05 18 0. 02 47 0. 94 78 Ta bl e 3. E ff ec ts o f t he m ai n fa ct or s on p la nt d ev el op m en t, a nd c on ce nt ra tio n an d to ta l c on te nt o f P a nd F e in p la nt s D ry m at te r (g p la nt –1 ) P (g k g– 1 ) To ta l P Fe To ta l F e (µ g pl an t– 1 ) P so ur ce Sh oo ts Ro ot s To ta l Sh oo ts Ro ot s Ro ot s (m g pl an t– 1 ) Sh oo ts (m g kg –1 ) Ro ot s (g k g– 1 ) Sh oo ts Ro ot s Ph os ph at e ro ck 0. 70 ± 0 .0 5 b 0. 12 ± 0 .0 1 b 0. 83 ± 0 .0 6 b 0. 99 ± 0 .0 4 a 0. 93 ± 0 .0 9 0. 11 ± 0 .0 2 b 66 ± 2 3 1. 05 ± 0 .1 0 a 48 ± 1 9 b 13 5 ± 11 0 b In s6 P† 0. 84 ± 0 .0 7 a 0. 16 ± 0 .0 1 a 0. 99 ± 0 .0 8 a 0. 87 ± 0 .0 2 b 0. 86 ± 0. 07 0. 13 ± 0 .0 1 ab 62 ± 2 5 0. 76 ± 0 .0 9 b 60 ± 3 3 ab 13 4 ± 10 4 b KH 2P O 4† 0. 95 ± 0 .0 6 a 0. 18 ± 0 .0 2 a 1. 13 ± 0 .0 7 a 1. 00 ± 0 .0 4 a 0. 88 ± 0 .0 6 0. 14 ± 0 .0 1 a 70 ± 3 1 1. 01 ± 0 .0 9 a 67 ± 2 3 a 18 4 ± 10 8 a In oc ul at io n + T3 4 0. 88 ± 0 .0 5 a 0. 16 ± 0 .0 1 1. 04 ± 0 .0 6 a 0. 91 ± 0 .0 2 b 0. 94 ± 0 .0 7 0. 14 ± 0 .0 1 a 67 ± 2 4 0. 98 ± 0 .0 9 64 ± 2 7 a 16 6 ± 11 0 a – T3 4 0. 78 ± 0 .0 5 b 0. 14 ± 0 .0 1 0. 93 ± 0 .0 7 b 1. 00 ± 0 .0 4 a 0. 84 ± 0 .0 4 0. 11 ± 0 .0 1 b 66 ± 2 2 0. 90 ± 0 .0 7 53 ± 2 5 b 13 7 ± 10 6 b M ea ns ± s ta nd ar d er ro r; n = 2 0 fo r P so ur ce ; n = 3 0 fo r in oc ul at io n; In s6 P = m yo -in os ito l-6 -h ex ak is ph os ph at e; † = A ds or be d on F e ox id es ; T he e ff ec ts w er e on ly c on si de re d w he n th e fa ct or c on ce rn ed ex hi bi te d no s ig ni fic an t i nt er ac tio n; M ea ns fo llo w ed b y di ff er en t l et te rs w ith in a c ol um n ar e si gn ifi ca nt ly d iff er en t a s pe r Tu ke y’ s te st a t p < 0 .0 5 fo r ea ch fa ct or CF U = c ol on y fo rm in g un its ; p v al ue s < 0. 05 in di ca te a s ig ni fic an t e ff ec t o f t he s ou rc e; C FU w as 0 fo r no n- in oc ul at ed p la nt s; th er ef or e, o nl y on e so ur ce (P s ou rc e) w as c on si de re d in th e an al ys is o f v ar ia nc e A G R I C U LT U R A L A N D F O O D S C I E N C E A. M. García-López et al. (2015) 24: 249–260 255 Acid phytase in the growth media after cultivation was greater with InsP6 than with the other P sources (p < 0.001; Table 4). pH was also significantly affected by P source (Table 2), with Ins6P leading to a lower value than PR (p < 0.01; Table 4). Inoculation with T34 increased DM (p < 0.01), total content of Fe in shoots (p < 0.01), and total contents of P and Fe in roots (p < 0.05) (Tables 2 and 3). Total P uptake was linearly related to total Fe uptake. However, linear re- gression had significantly lower slopes (p< 0.05) with T34 than without it (Fig. 1), thus revealing that P uptake was less markedly dependent on Fe uptake in the presence of T34 than in its absence. Table 4. Effects of the main factors on properties of the plant growing media P source Acid phytase (EU) kg-1 pH CFU Phosphate rock 0.5 ± 0.1 b 7.84 ± 0.04 a 9318 ± 2335 Ins6P† 3.8 ± 0.2 a 7.64 ± 0.06 b 9740 ± 2482 KH2PO4† 1.2 ± 0.3 b 7.73 ± 0.06 ab 5155 ± 1112 Inoculation + T34 2.1 ± 0.4 a 7.69 ± 0.04 b – T34 1.6 ± 0.4 b 7.78 ± 0.04 a Fe uptake (mg plant-1) 0,0 0,1 0,2 0,3 0,4 0,5 0,6 P up ta ke (m g pl an t- 1 ) 0,0 0,2 0,4 0,6 0,8 1,0 1,2 1,4 1,6 + T34 - T34 R2 = 0.63*** R2 = 0.30** Fig. 1. Relationships between total P uptake and total Fe uptake by cucumber plants. The dotted line represents the linear regression for plants without T34 inoculation (Y = 0.36 + 2.3 X) and the solid line that for inoculated plants (Y = 0.56 + 1.15 X). The intercept and slope of both regression lines were significantly different at p < 0.05 **. CFU = colony forming units; EU =enzyme units (amount of enzyme hydrolysing 1 μmol of P in 1 min); Ins6P = myo-inositol-6-hexakisphosphate; Means ± standard error: n = 20 for P source; n = 30 for inoculation; † = Adsorbed on Fe oxides; The effects were only considered when the factor concerned exhibited no significant interaction; Means followed by different letters in a column were significantly different as per Tukey’s test at; p < 0.05 for each factor A G R I C U LT U R A L A N D F O O D S C I E N C E A. M. García-López et al. (2015) 24: 249–260 256 T34 increased acid phytase activity and decreased pH after cultivation in the growth media (p < 0.05, in both cases; Ta- bles 2 and 4). The effect of T34 on alkaline phytase, total content of P in aerial parts and total P uptake by plants was de- pendent on P source as suggested by the significant interaction between both factors (p < 0.05; Table 2). With Ins6P, T34 led to a P uptake and total P content of aerial parts not significantly different from those obtained with KH 2 PO 4 (Table 5). Inoculation increased total P in aerial parts and total P uptake in the plants supplied with Ins6P (Table 5). In inoc- ulated plant growth media, total P uptake and P in shoots with PR was not significantly different from those with KH 2 PO 4 (Table 5). Phosphomonoesterase and organic anions in the growing media after cultivation were also affected by T34, but an opposite trend with PR vs other two P sources was observed explaining the significant interaction in both variables (Table 2). Phosphomonoesterase activity was linearly related to root development, measured as DM (R2 = 0.32; p < 0.001, n = 60), and to CFU of T34 in the media (R2 = 0.34; p < 0.05, n = 30; non- inoculated pots excluded). The contents in organic anions —oxalate accounted for the only one detected— were decreased by T34 with KH 2 PO 4 and Ins6P, which were the two P sources leading to the lowest concentrations of organic anions in the growth media, meanwhile the opposite effect was observed with PR (Table 5). Discussion Effect of T34 on plant development and nutrient uptake Trichoderma asperellum T34 increased the total content of P in roots of cucumber (Tables 2 and 3) irrespective of the particular P form used to supply this nutrient; also, it enhanced total P uptake by plants when the phosphorus was supplied as Ins6P or PR (Tables 2 and 5). This reveals a favourable effect of the fungus on the use of not read- ily phytoavailable P forms (e.g., Ins6P, poorly soluble Ca phosphates) by plants. The improvement of P uptake by T34 is in agreement with previous works with other Trichoderma spp. which revealed their abilities for solubilising insoluble phosphates (Altomare et al. 1999, Rudresh et al. 2005). In addition to P, T34 increased the total content of Fe in shoots and roots of cucumber plants (Tables 2 and 3), which suggests improved Fe uptake in media with restricted availability of this nutrient by effect of their ferrihydrite concentration and pH (de Santiago et al. 2009). The favourable effect of the fungus on development of cucumber plants measured as DM yield might be related to the improved P and Fe uptake observed with T34 in media with restricted availability of both nutrients (Table 3). Changes in phytohormone profile in plants (e.g. indole-3-acetic acid or 1-aminocyclopropane-1-carboxyl- ic acid; Verma et al. 2007, Martínez-Medina et al. 2011) can also contribute to the effect of T34 increas- ing plant development. However, this effect seems improbable because there was not a significant increase in plant DM by T34 when the most effective P source (KH 2 PO 4 ) was only taken into account (p = 0.4668). T34 did not promoted positive effects on P (p = 0.2403) or Fe uptake (p = 0.3943 and p = 4012 for total Fe in shoots and roots, respectively) by plants with KH 2 PO 4 . Thus, increased development by T34 seemed to be related to posi- tive effects on P and Fe nutrition more clearly observed in plants supplied with PR and Ins6P. Although an increased uptake of other nutrients different from P and Fe by T34 is possible, which can also contribute to the effect of the Table 5. Effect of the significant interactions between main factors on properties of the plant growth media and concentration and total content of P in plant P source Inoculation Total P uptake‡ Total P shoots Phospho- monoesterase Alkaline phytase Organic anions mg plant–1 mg pNP kg–1 h–1 EU kg–1 mmol kg–1 Phosphate rock + T34 0.77 ± 0.08 bc 0.73 ± 0.06 ab A§ 5.21 ± 1.33 2.0 ± 0.1 a 47 ± 15 a A – T34 0.59 ± 0.07 c 0.62 ± 0.07 b B 4.02 ±0.08 2.5 ± 0.3 a 32 ± 10 ab B Ins6P† + T34 0.79 ± 0.07 abc A 0.77 ± 0.07 ab A§ 3.72 ± 0.57 1.5 ± 0.07 b A 6 ± 2 c – T34 0.54 ± 0.10 c B 0.56 ± 0.09 b B 4.86 ± 1.1 1.2 ± 0.07 c B 16 ± 5 ab KH2PO4† + T34 0.92 ± 0.09 ab 0.87 ± 0.08 a 4.11 ± 0.83 1.3 ± 0.1 bc 7 ± 1 bc B – T34 1.01 ± 0.07 a 0.98 ± 0.06 a 5.07 ± 0.93 1.2 ± 0.09 c 12 ± 2 ab A Mean ± standard error, n = 10 ; Ins6P = myo-inositol-6-hexakisphosphate; EU = enzymatic units (amount of enzyme hydrolysing 1 μmol P in 1 min); pNP = p-nitrophenol ; † = Adsorbed on Fe oxides; Means followed by a different lower case letter in a column were significantly different as per Tukey’s test at p < 0.05 for the six combinations of treatments; means followed by a different upper case letter in a column were significantly different as per Tukey’s test at p < 0.05 for the same P source; §P = 0.0761 and p = 0.0590 for Ins6P and PR, respectively.; ‡ = Estimated as total P in aerial parts and roots minus P present in seeds. Total P in shoots was occasionally greater than total P uptake because P in seeds was not counted for P determination in aerial parts A G R I C U LT U R A L A N D F O O D S C I E N C E A. M. García-López et al. (2015) 24: 249–260 257 fungi increasing plant development (Yedidia et al. 2001, de Santiago et al. 2013), it seems unlikely because a suf- ficient supply of these nutrients is guaranteed through nutrient solution. Iron mobilization strategies of plants and microorganisms involve the exudation of organic anions with Fe-compl- exation abilities (e.g., citrate) and acidification (Jones 1998, Marschner et al. 2011). This additionally contributes to increasing P availability to plants through desorption from oxides or dissolution of precipitated phosphates. Therefore, P uptake by plants may be related to Fe uptake by plants. However, the relationship between P uptake and Fe uptake was found to differ depending on whether the growth medium was inoculated with T34 (Fig. 1). This result suggests the involvement of different Fe and P release mechanisms when T34 is present in the growth medium, e.g. more specific mechanisms for Fe release by the fungus (e.g., siderophore production; (de Santiago et al. 2009), which can also provide more available Fe to plants with less P release from oxides. Enzymatic hydrolytic activities in growing media The improved P uptake by plants in T34-containing media where P was supplied in organic form (Ins6P) might be related to production of extracellular hydrolytic enzymes involved in the P cycle. This hydrolytic capacity was re- flected in the phytase activity and MRP concentration observed in liquid pure cultures of T34 with Ins6P as the P source (Table 1) and in the increased acid phytase activity in plant growing media containing the fungus (Table 4). Also, alkaline phytase activity was increased by T34, but only when P was supplied to plant growth medium as Ins6P. The effect of T34 on phosphomonoesterase activity in the plant growing media showed different trends depend- ing on P source (significant interaction) and peaked with PR (Table 5). This P source was expected to lead to the lowest MRP concentrations in solution judging by the results with the liquid culture (Table 1). There is contribu- tion of plants to such activity in the rhizosphere because 32% of all phosphomonoesterase activity was explained by root development, measured as DM; on the other hand, 34% of this enzymatic activity was explained by T34 development, measured as CFU in the media. An increased phosphomonoesterase activity, which is related to the hydrolysis of monoester phosphates other than Ins6P, suggests the presence of an additional mechanism for T34 to facilitate P nutrition by plants in soil. This activity is known to be involved in the turnover of organic P in the rhizosphere (Turner and Haygarth 2005). Phosphomonoesterase activity due to T34 was seemingly more strongly affected by the inorganic P concentra- tion in solution than by P source. Thus, it was lower in the liquid T34 culture with the source leading to the high- est MRP concentration in the medium (Table 1); by contrast, phytase activity was apparently more markedly af- fected by the dominant P form present in the medium (Table 1). Phytase activities were greatest with Ins6P and full strength KH 2 PO 4 in the T34 liquid cultures. It is known that phytase activity can increase at increased substrate concentration, but also at increased KH 2 PO 4 in pure cultures (Chun et al. 2007). These results are consistent with those in the plant growing media, where acid phytase activities peaked with Ins6P (Table 4). Adsorption of fungal (acid) phytases on Fe oxides markedly reduces their activity (George et al. 2005). This activity is known to be in- creased by Ins6P by effect of adsorption of this P form on Fe oxides decreasing adsorption of phytase and increas- ing its catalytic activity over time as a result (Giaveno et al. 2010). This contributes to explain the increased acid phytase activity observed with Ins6P as P source in the plant growth media containing Fe oxides. Although inor- ganic P is also adsorbed on Fe oxides, sorbent surfaces have a higher affinity for Ins6P than for inorganic P (Berg and Joern 2006) as revealed in the present study by the much lower P concentration in equilibrium with InsP6 (3 µg L–1) than with phosphate (1.5 mg L–1) adsorbed on ferrihydrite. This accounts for the increased inhibition of acid phytase adsorption and hence the greater activity of this enzyme in solution observed with Ins6P relative to KH 2 PO 4 (Table 4). An increased affinity of oxides for Ins6P relative to inorganic phosphate accounts for the de- creased Fe accumulation in roots with the former P source compared to KH 2 PO 4 since Ins6P inhibits adsorption of complexing organic compounds released by roots or T34 on Fe oxides, a requirement for efficient Fe release from Fe-rich particles (Borer et al. 2009). Acidification and organic anions in growth media The increased use of P from PR might be related to the acidification of the rhizosphere and/or the production of low-molecular weight organic anions facilitating dissolution of Ca phosphates (Reyes et al. 1999, Hariprasad and Niranjana 2009). These effects were observed in the pure culture of T34 (Table 1); also, acidification of the rhizo- sphere and increased concentration of organic anions with PR by T34 was observed in the plant growing media (Tables 4 and 5). A G R I C U LT U R A L A N D F O O D S C I E N C E A. M. García-López et al. (2015) 24: 249–260 258 Oxalate was the dominant organic anion in the plant growth media. This is unsurprising since oxalate is typically the dominant anion exuded by roots of cucumber (El-Baz et al. 2004) and other plants (Jones 1998, Ström et al., 2005) under conditions of Fe or P deficiency. Furthermore, microbial degradation in the rhizosphere can have a lesser effect on oxalate than on other organic anions (Ström et al. 2001). A much higher organic anion concentra- tion was found with PR than with Ins6P or KH 2 PO 4 (Table 5). In spite of the organic anion production by T34 ob- served in the pure liquid culture (Table 1), the effect of the fungus was not always positive in plant growth media. In these media, T34 increased the concentration of organic anions with PR, thus contributing to dissolve PR, but decreased it with Ins6P and KH 2 PO 4 as revealed by the significant interaction between both factors (Tables 2 and 5). This can be explained at least in part by the production of organic anions by plants. Plants and T34 seemingly increased organic anions concentrations to a greater extent with PR than with other P sources because this strategy is essential to dissolve sparingly soluble phosphates (Ström et al. 2005). With other P sources, T34 improves P uptake by plants through other mechanisms (e.g., hydrolytic activity with Ins6P); this can lead to a decreased exudation of organic anions by roots in order to mobilize P, which is highly costly in energetic terms (Ström et al. 2002, 2005). Conclusions Trichoderma asperellum T34 increased the total content in P of cucumber roots and enhanced total P uptake by plants when the phosphorus was supplied as Ins6P or PR. This was mainly explained by the production of extra- cellular hydrolytic enzymes involved in the P cycle, rhizosphere acidification, and increased organic anion concen- tration with T34. 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Effect of Trichoderma harzianum on microelement concentrations and in- creased growth of cucumber plants. Plant and Soil 235: 235–242. Plant uptake of phosphorus from sparingly available P- sources asaffected by Trichoderma asperellum T34 Introduction Materials and methods Experimental setup Cultivation of T34 in liquid media Inoculation experiments with plants Enzyme activity determination Organic anion determination Plant analysis Determination of T34 density in growing media Statistical analysis Results Cultivation of T34 in liquid media Experiments with plants Discussion Effect of T34 on plant development and nutrient uptake Enzymatic hydrolytic activities in growing media Acidification and organic anions in growth media Conclusions Acknowledgement References