A G R I C U LT U R A L A N D F O O D S C I E N C E A. Pasqualone et al. (2015) 24: 118–127 118 Quality characteristics of parental lines of wheat mapping populations Antonella Pasqualone1, Luciana Piarulli2, Giacomo Mangini2, Agata Gadaleta2, Antonio Blanco2 and Rosanna Simeone2 1Food Science and Technology Unit, University of Bari, Department of Soil, Plant and Food Sciences, Via Amendola, 165/A, I 70126, Bari, Italy 2Genetics and Plant Breeding Unit, University of Bari, Department of Soil, Plant and Food Sciences, Via Amendola, 165/A, I 70126, Bari, Italy email: antonella.pasqualone@uniba.it The aim of this work was to evaluate the main quality traits in the parental lines of wheat segregating populations to identify the best for subsequent genetic mapping of the traits. Significant differences (p < 0.001) among wheat genotypes were observed. Many of the examined crosses appeared to be suitable for the purpose, showing differ- ences among parental lines as high as 4.6% for protein content, 6.4% for gluten content, 69 for gluten index, 50 mL for sodium dodecyl sulphate sedimentation volume, and 33.9 g for thousand-kernel weight, whereas differences accounting for 4.8, 2.4, and 7.3 were observed for yellow index, red index and brown index, respectively. The re- sults pointed out that for studying at the same time the quantitative and qualitative features of gluten, the wheat populations derived from Latino x MG29896 and Saragolla x 02-5B-318 could be particularly appropriate. In addi- tion, the latter cross was suitable to deepen the knowledge of yellow index regulation. Key words: wheat, whole meal, mapping population, protein, gluten Introduction The most important quality characteristics for both bread and pasta are the textural and color features. The first, both in soft and durum wheat, are strongly related to the quali-quantitative characteristics of gluten-forming pro- teins, which properties allow wheat flour to be transformed into bread or pasta (Goesaert et al. 2005). Moreover, when high-temperature drying technology is used, protein content remains the most important parameter influ- encing the cooking quality of pasta (D’Egidio et al. 1990). Color influences consumer choice at the moment of purchase: bright yellow pasta, arising from carotenoid pig- ments of durum wheat semolina (Feillet et al. 2000) is preferred over pale or discolored products. In addition, carotenoids increase the nutritional value (Garcia-Casal 2006). Similarly, when semolina is used in bread-making, as usually made in Southern Italy (Pasqualone 2012) and in Mediterranean countries (Qaroni 1996), its pigments confer a distinctive and appreciated yellowish tone to bread crumb (Pasqualone et al. 2004, 2007). Undesired color alterations, mainly browning, can derive from Maillard’s reaction or enzymatic activity. Polyphenol oxidase, in particular, abundant in the varieties richer of phenolics (Pasqualone et al. 2014), is correlated with both dough darkening (Taranto et al. 2012) and color alterations of noodles (Fuerst et al. 2006) and bread (McCallum and Walker 1990). On the other hand, pigmented wheats - red, purple, or blue - are becoming the object of interest due to their high anthocyanin content (Ficco et al. 2014). Many breeding efforts have been devoted to increase the quality features of wheat. In particular, genetic link- age maps, that locate genes related to traits of interest, are an useful tool in breeding programs. The availability of markers linked to quantitative trait loci (QTLs) facilitates the genetic dissection of quantitative traits and the early selection in wheat breeding programs. Detecting QTLs by molecular markers involves the analysis of popu- lations constituted by large number of individuals which segregate concurrently for marker loci and quantitative trait expression. Double haploid and recombinant inbred populations are particularly suited for this purpose as they can be characterized for additional loci at any time and analyzed in combination with the previously accu- mulated data, assumed that parental lines are sufficiently differentiated for the traits of interest, so as to ensure segregation. Such lines constitute permanent mapping populations that may be evaluated over space and time (Mather and Jinks 1971). Manuscript received March 2015 A G R I C U LT U R A L A N D F O O D S C I E N C E A. Pasqualone et al. (2015) 24: 118–127 119 Previous papers have reported the effective detection and mapping of quantitative trait loci (QTLs) related to protein content (Blanco et al. 2002), sodium dodecyl sulphate sedimentation volume (Blanco et al. 1998), yellow pigments (Blanco et al. 2011) and yield components (Blanco et al. 2001) in recombinant inbred populations of tetraploid wheat. However, some genes are cultivar specific, hence it is needed to investigate on them in several mapping populations (Bernardo 2008). The aim of this work was to evaluate the main quality traits (thousand-kernel weight, protein content and compo- sition, quali-quantitative features of gluten, color indices) of parental lines of existing wheat mapping populations to identify the best to be used for subsequent mapping of the traits of interest in the derived segregating progeny. Material and methods Samples Twenty-nine wheat accessions and cultivars, reported in Table 1,were considered. All of them were parental lines of segregating populations set up at the Genetics and Plant Breeding Unit, Department of Soil, Plant and Food Sci- ences (DISSPA), University of Bari (Italy), for a total of 28 crosses, as specified in Table 2. The plants were grown in the experimental field of the Department of Soil, Plant and Food Sciences at Valenzano (Bari, Italy) in 2013, in a randomized complete block design with three field replicates and plots consisting of 1-m rows, 30 cm apart, with 50 germinating seeds per plot. During the growing season, 120 kg ha-1 N were applied and standard cultivation practices were adopted. Plots were hand-harvested at maturity. A seed sample (15 g) per plot was used to deter- mine the thousand-kernel weight (KW). Basic chemical and physical determinations Harvested grain samples from each plot were separately milled to whole meal on a laboratory mill equipped with 1-mm sieve (Cyclotec Sample Mill, Tecator Foss, Hillerød, Denmark). Moisture content was determined at 105 °C by means of an automatic moisture analyzer (Radwag Wagi Elektroniczne, Radom, Poland). Grain protein content (GPC) was determined by using a dual beam near infrared reflectance spectrophotometer (Zeutec Spectra Alyz- er Premium, Zeutec Büchi, Rendsburg, Germany). Colorimetric evaluations of yellow index (YI, corresponding to b*), red index (RI, a*), and brown index (BI, defined as 100-L*), were carried out by means of the reflectance col- orimeter Chroma Meter CR-300 (Konica Minolta Sensing, Osaka, Japan). Whole meal was placed into the granu- lar materials attachment CR-A50 (Konica Minolta Sensing, Osaka, Japan) of the colorimeter to obtain a smooth surface suitable for color readings. Results were the average of two analytical replications apart for colorimetric evaluations that were replicated 5 times. Table 1. Taxonomic classification of the wheat cultivars and accessions considered Taxonomic classification Type of plant material Triticum aestivum L. ssp. aestivum Chinese Spring Cultivar Chinese Spring - 5A dic. Chinese Spring disomic substitution line of 5A chromosome with the accession TA106 of ssp. dicoccoides 02-5B-318 Breeding line Triticum turgidum ssp. dicoccum (Schrank ex Schϋbler) Thell. MG5323 Landrace Triticum turgidum ssp. dicoccoides (Körn. ex Asch. & Graebner) Thell. MG4343; MG4330; MG29896 Wild accessions Triticum turgidum ssp. durum (Desf.) Husnot CItr 14629 Purple line derived from an Ethiopic landrace UC1113 UC Davis breeding line with the high grain protein content gene GpcB1 Anco Marzio; Aureo; AC Avonlea; Ciccio; Duilio; Fiore; Grecale; Iride; Isildur; Latino; Latinur; Liberdur; Messapia; Neolatino; Normanno; Preco; Primadur; Saragolla; Svevo; Tiziana Cultivars A G R I C U LT U R A L A N D F O O D S C I E N C E A. Pasqualone et al. (2015) 24: 118–127 120 Determination of quali-quantitative features of gluten Wet gluten (WG) and gluten index (GI) were determined according to the ICC Standard No. 155 (ICC 1994) by means of the complete system consisting of Glutomatic 2200, Centrifuge 2015, and Glutork 2020 (Perten Instru- ments AB, Huddinge, Sweden). First, WG was recovered by means of Glutomatic 2200 (Perten Instruments AB, Huddinge, Sweden). Then, GI was calculated as the percent ratio of the WG fraction remaining on the sieve after centrifugation (Centrifuge 2015, Perten Instruments AB, Huddinge, Sweden) to the total WG weight. Dry gluten (DG) was determined by drying the total WG at 150 °C for 4 min by means of Glutork 2020 apparatus (Perten In- struments 0AB, Huddinge, Sweden). Gluten hydration index (GHI) was calculated as the percent ratio (WG - DG)/ WG. Results were the average of two analytical replications. Sedimentation volume in sodium dodecyl sulphate (SDS-SV) The test of SDS-SV was performed according to the ICC Standard method no. 151 (ICC 1990). Results were the av- erage of two analytical replications. Electrophoretic analyses of seed storage proteins Gliadins and glutenins were extracted from 60 mg whole meal flour and analyzed by polyacrylamide gel electro- phoresis in acidic conditions (A-PAGE) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), respectively, on a Protean II apparatus (Bio-Rad, Hercules, CA), according to the conditions of Lukow et al. (1990). Statistical analyses Standard ANOVA (with genotype as fixed factor and replication as random factor) was performed using the soft- ware MSTAT-C (Michigan State University, East Lansing, MI). The differences (Δ) between pairs of parental lines of segregating populations were calculated and the Fisher’s least significant difference at 5% significance level (LSD 0.05 ) was applied to point out the most polymorphic pairs for the traits analyzed. Pearson phenotypic correla- tion coefficients (R) were calculated among all traits. Results and discussion Variability of quality characteristics and correlations among them Glutenins and gliadins encoded at the Glu-1 and Gli-1 loci are the most commonly considered in wheat breeding pro- grams aimed to improve gluten quality. The glutenin composition, in particular, has been used to establish a quality score, as extensively reviewed by Shewry et al. (1992). The allelic variations of high molecular weight glutenin sub- aGadaleta et al. 2012, 2014; bPiarulli et al. 2012; cBlanco et al. 1998, 2001, 2002; dBlanco et al. 2011; eBlanco et al. 2012. Table 2. Pairs of parental lines and progeny size of 28 wheat mapping populations set up at the Genetics and Plant Breeding Unit, Department of Soil, Plant and Food Sciences, University of Bari, Italy Cross Progeny individuals (no.) Cross Progeny individuals (no.) Aureo x Normanno 896 Iride x Fiore 180 Aureo x Iride 616 Svevo x MG4330 177 Saragolla x 02-5B-318 421 Grecale x Tiziana 160 Neolatino x Preco 409 Iride x Ciccio 160 Latinur x Saragolla 405 Normanno x Fiore 160 Isildur x Saragolla 405 Duilio x AC Avonlea 150 Liberdur x Saragolla 324 CItr 14629 x Grecale 148 Svevo x Primadur 322 Latino x MG29896 144 UC1113 x Iride 320 Grecale x Fiore 130 Isildur x Anco Marzio 308 Latino x MG5323b 122 Liberdur x Anco Marzio 296 Messapia x MG4343c 122 Normanno x Tiziana 260 Latino x Primadurd 121 Svevo x Messapia 249 Svevo x Ciccioe 120 Chinese Spring x Chinese Spring - 5A dic.a 188 Iride x Tiziana 90 A G R I C U LT U R A L A N D F O O D S C I E N C E A. Pasqualone et al. (2015) 24: 118–127 121 units (HMW-GS) and of ω- and γ-gliadins in the wheat cultivars and accessions considered are reported in Table 3. Three different HMW-GS alleles at the Glu-A1 locus, 5 HMW-GS alleles at the Glu-B1 locus, and 5 different gliadin alleles at the Gli-B1 locus, were observed. The number of alleles at each loci decreased to 2 if durum wheat cul- tivars only were considered. Due to the breeding process, the majority of cultivars contained HMW-GS 7+8 sub- units, that are characterized by a higher quality score than 6+8 or 20 subunits (Boggini and Pogna 1989, Shewry et al. 1992). The Triticum aestivum cultivar Chinese Spring and its disomic substitution line showed a 2+12 pat- tern at the Gli-D1 locus, known to be related to lower gluten quality if compared to the 5+10 combination. Latino was the only cultivar characterized by γ-42 gliadin. With few exceptions (Pogna et al. 1990), this gliadin is associ- ated to type-1 low molecular weight glutenin subunits (LMW-GS 1), related to low technological quality (Boggini and Pogna 1989). The γ-45 gliadin, usually related to type-2 LMW-GS and considered an easily detectable quality marker (Boggini and Pogna 1989), was observed in the improved Neolatino cultivar (derived from Latino) and in all the other cultivars. In addition, Neolatino showed also the HMW-GS 2* subunit, that positively influences qual- ity more than the null allele that, instead, characterized all the other cultivars. As expected, the landrace MG5323 and the wild accessions MG4343, MG4330, and MG29896 showed the most differentiated patterns in terms of both gliadins and HMW-GS. The analysis of variance carried out on GPC, quali-quantitative characteristics of gluten, SDS-SW, KW, and color indices of parental lines of wheat mapping populations revealed highly significant differences (p < 0.001) among genotypes for all the traits (Table 4). GPC and gluten quality of wheat are the most important factors affecting pasta consistency and resistance to over- cooking (D’Egidio et al. 1990) and bread volume (Goesaert et al. 2005). In the examined set of 29 wheat acces- sions and cultivars, the GPC varied in the range comprised between 14.0% and 19.5% (Table 5). As expected for wild materials, the highest values were observed in ssp. dicoccoides, and confirmed the levels ascertained in the same materials in previous researches (Taranto et al. 2012). Among the durum cultivars, Preco showed the high- est value of GPC, and also the line CItr 14629 showed a considerably high level of proteins. aAt Glu-D1 locus the subunits 2+12 were observed. n.d. = not determined. Table 3. Allelic composition of high molecular weight glutenin subunits (HMW-GS) and of ω- and γ-gliadins, encoded at Glu-A1, Glu-B1, and Gli-B1 loci, detected in wheat cultivars and accessions Parental line Locus Glu-A1 Glu-B1 Gli-B1 HMW-GS subunit composition Allele code HMW- GS subunit composition Allele code Gliadin composition Allele code Chinese Springa null c 7+8 b ω-31-35-38, γ-42 Chinese Spring - 5A dic.a null c 7+8 b ω-31-35-38, γ-42 02-5B-318 n.d. n.d. n.d. MG5323 1 a 22 k ω -31-33, γ-45 MG4343 1 a 18+23 ω -31-33, γ-45 MG4330 2* b 20 e ω-31-35-38, γ-45 MG29896 1 a 6+8 d ω-31, γ-45 Cltr 14629 n.d. n.d. n.d. UC1113 null c 7+8 b ω-35, γ-45 c Anco Marzio null c 7+8 b ω-35, γ-45 c Aureo n.d. 6+8 ω-35, γ-45 AC Avonlea n.d. n.d. ω-35, γ-45 Ciccio null c 7+8 b ω -35, γ-45 c Duilio null c 7+8 b ω -35, γ-45 c Fiore null c 7+8 b ω -35, γ-45 c Grecale null c 6+8 d ω-35, γ-45 c Iride null c 7+8 b ω-35, γ-45 c Isildur n.d. n.d. n.d. Latino null c 7+8 b ω-33-35-38, γ-42 a Latinur null c 7+8 b ω-35, γ-45 c Liberdur n.d. 20 ω-35, γ-45 Messapia null c 6+8 d ω-35, γ-45 c Neolatino 2* b 7+8 b ω-35, γ-45 c Normanno null c 7+8 b ω-35, γ-45 c Preco null c 6+8 d ω-35, γ-45 c Primadur null c 6+8 d ω-35, γ-45 c Saragolla null c 6+8 d ω-35, γ-45 c Svevo null c 7+8 b ω-35, γ-45 c Tiziana null c 7+8 b ω-35, γ-45 c A G R I C U LT U R A L A N D F O O D S C I E N C E A. Pasqualone et al. (2015) 24: 118–127 122 The portion of total protein represented by gluten showed a high variability among the tested whole meals. Due to its strong correlation with GPC (R = 0.78, p <0.001, and R = 0.86, p <0.001, for WG and DG, respectively) (Table 6), gluten content paralleled the trend of proteins, with higher values in ssp. dicoccoides and in CItr 14629 than in the cultivars. High DG and WG contents were observed also in Chinese Spring and Chinese Spring - 5A dic. The lowest DG to GPC ratio was observed in cv. Saragolla, while the highest values were reached in Chinese Spring, Chinese Spring - 5A dic. and in MG5323 line. DG varied from 8.0% (cv. Saragolla) to 15.7% (CItr 14629), with a GHI ranging from 66.8 (cv. Aureo) to 72.8 (Chi- nese Spring - 5A dic.). A positive correlation was observed between DG and GHI (R = 0.64, p < 0.001). GI is widely used for evaluating gluten strength (GI < 30 = weak; 30–80 = normal; > 80 = strong) (Cubadda et al. 1992). It is known to correlate with SDS-SV, alveograph work (Cubadda et al. 1992), loaf volume (Kieffer et al. 1998), and mixograph peak time (Gaines et al. 2006). This parameter reproduces the manual gluten quality eval- uation, but being instrumentally determined avoids any influence of the operator on the results. In addition, it has been reported to have high heritability (Ames et al. 1999) and, due to the relatively small-scale and rapidity of the method used for its determination, is a reliable predictor of gluten strength for screening early breeding generations (Sissons et al. 2005). The GI of the examined wheat samples was negatively correlated with WG and DG content (R = -0.81, p < 0.001 and R = -0.67, p < 0.001, respectively), confirming the findings of Cubadda et al. (1992) and Peña (2000), so that higher WG and DG levels corresponded to lower gluten strength. Furthermore, a negative correlation was ob- served between GI and GHI (R = -0.97; p <0.001), i.e. stronger the gluten, lower the hydration. Very low GI values, indicating a very weak and sticky gluten, insufficient for both bread-making and pasta produc- tion, were observed in the dicoccum accession MG5323, in the durum line CItr 14629, and in cv. Latino. Among the T. aestivum samples, Chinese Spring and its disomic substitution line Chinese Spring 5A-dic. also showed weak gluten, in agreement with their protein allelic composition. The highest values of GI (≥90) were observed in Fiore, Neolatino, Normanno, and Saragolla durum cultivars, indicating the presence of strong gluten network with op- timal pasta-making properties (Sissons et al. 2005). The very low and very high GI values observed in Latino and Neolatino, respectively, agreed with the differences observed in their protein allelic composition. An intermediate behavior was assessed in cv. AC Avonlea, in the accession MG4330 and in the line 02-5B-318, while all the other cultivars and accessions showed GI values higher than 60. Besides the production of pasta, durum wheat bread-making is an established tradition in Southern Italy and in Mediterranean countries (Qaroni 1996, Pasqualone 2012). Apart the above cited few cases, characterized by ex- tremely low GI values, the majority of the whole meals tested appeared to be able to ensure good bread-making performances. In fact, according to Curic et al. (2001), GI values between 75 and 90 provide optimal bread-mak- ing quality, while Har Gil et al. (2011) indicate that for bread-making is sufficient a value above 55. The observed values were similar to those ascertained in a previous survey on the quality of durum wheat semolina used for bread-making in Southern Italy (Pasqualone et al. 2004). The SDS-SV ranged from 31 mL to 82 mL and was significantly correlated with GI (R = 0.65, p < 0.001). The majority of the cultivars of both ssp. aestivum and ssp. durum showed SDS-SV values exceeding 70, with the highest value in the breeding line 02-5B-318 for ssp. aestivum and in cv. Primadur for ssp. durum. Lower values were observed in ssp. dicoccoides and ssp. dicoccum samples, with the exception of MG29896. The SDS-SV test is based on the prop- erty of the gluten-forming endosperm storage proteins to swell and flocculate in a weak acid solution (lactic acid). **, *** indicate significant differences at p < 0.01 and p < 0.001, respectively. The ANOVA was carried out on 29 genotypes. The accession MG4343 has not been included in the statistical analyses due to incomplete data. Table 4. Results of the analysis of variance carried out on grain protein content (GPC), wet gluten (WG), dry gluten (DG), gluten hydration index (GHI), dry gluten/protein ratio (DG/GPC), gluten index (GI), sodium dodecyl sulphate sedimentation volume (SDS- SV), thousand-kernel weight (KW), and color indices (YI = yellow index; RI = red index; BI = brown index) of whole meal of parental lines of wheat mapping populations grown in Valenzano (Bari, Italy) in 2013 Source of variation d.f. GPC WG DG GHI DG/GPC GI SDS-SV KW YI RI BI Replication 2 18.64*** 442.59*** 44.14*** 3.35** 474.11*** 103.25 515.33*** 94.30** 1.79** 0.54*** 2.91*** Genotype 28 4.40*** 267.82*** 15.45*** 11.07*** 294.65*** 2101.22*** 573.54*** 225.19*** 13.25*** 1.20*** 8.78*** Error 55 0.55 10.88 0.84 0.55 22.30 36.72 31.94 7.93 0.33 0.05 0.29 A G R I C U LT U R A L A N D F O O D S C I E N C E A. Pasqualone et al. (2015) 24: 118–127 123 The rate of sedimentation is influenced by gluten strength: meals containing better quality gluten show slower rates and higher SDS-SV values (Axford et al. 1979). This index is correlated with bread-making quality (Axford et al. 1979), as well as with spaghetti cooking quality (Dick and Quick 1983), hence it is widely used to evaluate flour quality in both durum and soft wheat. KW values ranging from 25.3 g to 61.9 g were observed, higher in durum cultivars than in the other wheats consid- ered. Among the ssp. dicoccoides an interesting high value of KW was assessed, accounting for 48.5 g, in MG29896. Values above 50 g were observed in almost all the durum cultivars, with the highest value in cv. Duilio. n.d. = not determined for insufficient seed Table 5. Grain protein content (GPC), wet gluten (WG), dry gluten (DG), gluten hydration index (GHI), dry gluten/protein ratio (DG/GPC), gluten index (GI), sodium dodecyl sulphate sedimentation volume (SDS-SV), thousand-kernel weight (KW), and color indices (YI = yellow index; RI = red index; BI = brown index) of whole meal of parental lines of wheat mapping populations grown in Valenzano (Bari, Italy) in 2013 (mean of three field replications) Cultivar or accession GPC (% d.m.) WG (% d.m.) DG (% d.m.) GHI DG/GPC GI SDS- SV (mL) KW (g) YI RI BI Triticum aestivum L. ssp. aestivum Chinese Spring 16.4 57.0 15.6 72.6 95.0 25 61 31.1 10.5 1.1 14.2 Chinese Spring – 5A dic. 16.1 55.1 15.0 72.8 93.5 28 75 29.7 10.0 0.9 13.6 02-5B-318 16.9 50.1 14.4 71.3 85.2 47 79 39.6 10.6 1.5 15.6 Triticum turgidum ssp. dicoccoides (Körn. ex Asch. & Graebner) Thell. MG29896 18.8 50.2 16.1 67.9 85.5 75 68 48.5 14.9 1.0 16.2 MG4343 19.5 n.d. n.d. n.d. n.d. n.d. 46 25.3 12.3 1.8 19.3 MG4330 17.7 54.0 15.6 71.0 88.3 46 59 42.0 12.9 1.7 17.9 Triticum turgidum ssp. dicoccum (Schrank ex Schϋbler) Thell. MG5323 17.8 55.0 15.5 71.8 87.3 19 36 37.3 13.6 2.0 18.7 Triticum turgidum ssp. durum (Desf.) Husnot CItr 14629 17.5 55.7 15.7 71.9 89.8 23 31 46.8 11.5 2.5 23.0 UC1113 16.3 39.5 12.9 67.3 78.9 87 64 50.2 14.1 0.6 15.2 Anco Marzio 15.1 33.3 10.7 67.8 71.2 87 61 50.4 14.2 0.7 15.4 Aureo 16.8 37.1 12.3 66.8 73.6 87 77 55.9 15.2 0.4 15.8 AC Avonlea 16.2 48.4 14.1 70.9 87.2 38 54 48.7 16.7 0.0 15.1 Ciccio 15.6 36.2 11.5 68.3 73.4 78 70 60.2 14.4 0.4 15.9 Duilio 15.8 34.8 11.1 68.0 70.0 80 58 61.9 13.7 0.7 15.6 Fiore 14.0 25.1 8.2 67.4 58.4 92 60 46.5 13.4 0.4 14.6 Grecale 15.2 38.9 12.2 68.6 80.6 66 62 44.5 16.1 0.1 15.7 Iride 14.2 30.0 9.5 68.3 66.7 88 59 53.8 14.5 0.5 15.4 Isildur 14.8 36.3 11.7 67.7 78.8 84 74 50.4 17.8 0.0 16.0 Latino 14.2 40.0 11.2 71.9 78.1 17 32 60.9 12.7 0.4 14.7 Latinur 15.4 40.9 12.7 69.1 82.1 69 59 54.3 15.5 0.3 16.1 Liberdur 14.3 34.7 11.3 67.5 78.7 85 73 52.3 17.2 0.2 15.9 Messapia 16.1 40.9 12.7 68.8 79.1 68 75 59.2 12.8 0.8 15.5 Neolatino 15.7 33.6 10.8 67.7 69.2 90 73 58.8 13.2 0.4 15.0 Normanno 14.4 28.0 9.2 67.4 63.1 91 71 51.2 16.6 0.3 15.9 Preco 17.5 50.1 15.4 69.2 88.0 59 65 54.3 16.8 0.1 16.0 Primadur 15.8 34.3 11.2 67.2 70.5 86 82 35.0 17.6 0.1 15.6 Saragolla 14.6 24.8 8.0 67.7 54.6 92 65 51.0 15.4 0.5 15.6 Svevo 14.6 35.3 11.1 68.6 75.5 65 69 55.6 13.8 0.6 15.6 Tiziana 15.0 37.3 11.8 68.5 78.0 76 66 52.7 14.5 0.4 15.1 A G R I C U LT U R A L A N D F O O D S C I E N C E A. Pasqualone et al. (2015) 24: 118–127 124 Table 6. Pearson correlation coefficients (R) among protein content (GPC), wet gluten (WG), dry gluten (DG), gluten hydration index (GHI), dry gluten/protein ratio (DG/GPC), gluten index (GI), sodium dodecyl sulphate sedimentation volume (SDS-SV), thousand- kernel weight (KW), and color indices (YI = yellow index; RI = red index; BI = brown index) of parental lines of wheat mapping populations grown in Valenzano (Bari, Italy) in 2013 GPC WG DG GHI DG/GPC GI SDS-SV KW YI RI WG 0.78*** DG 0.86*** 0.97*** GHI 0.34 0.80*** 0.64*** G/GPC 0.65*** 0.95*** 0.94*** 0.72*** GI -0.39* -0.81*** -0.67*** -0.97*** -0.75*** SDS-SV -0.09 -0.33 -0.21 -0.57** -0.23 0.65*** KW -0.33 -0.50** -0.44* -0.42* -0.43* 0.34 -0.12 YI -0.24 -0.49** -0.35 -0.68*** -0.37 0.58** 0.29 0.26 RI 0.56** 0.64*** 0.56** 0.65*** 0.45* -0.63*** -0.52** -0.31 -0.72*** BI 0.44* 0.38* 0.37* 0.28 0.26 -0.36 -0.56** -0.01 -0.09 0.69*** Wheat kernel weight and size are important yield and quality traits, always considered primary objectives in con- ventional and modern wheat breeding programs. KW can be easily measured and is usually used to estimate the agronomic performance of a cultivar (Baril 1992). Moreover, it is positively correlated with high flour yield and is a cheap predictor of milling quality in bread wheat (Berman et al. 1996) and of semolina yield in durum wheat (Novaro et al. 2001). Regarding color indices, as expected the YI (range 10.0 – 17.8) was lower in T. aestivum samples than in tetraploid wheats due to the presence, in the latter, of carotenoid pigments that are strongly correlated with yellow hue (Digesù et al. 2009). Some durum cultivars, namely Isildur, Primadur, Liberdur, Preco, and AC Avonlea, showed particularly high values of YI, due to the effect of breeding for this valuable characteristic. Both ssp. dicoccoides and ssp. dicoccum samples showed values in-between T. aestivum and durum cultivars. The values of RI were generally quite low (range 0.0 – 2.5), with the highest values in CItr14629, that is a purple wheat line characterised by high anthocyanin levels (Pasqualone et al. 2015). Although not imputable to antho- cyanins, high values of RI were observed in MG5323, MG4343, and MG4330, probably originated from the oxida- tion of moderate levels of phenolic substances. The highest BI, reaching the value of 23.0, was observed in CItr14629. The origin of inherent brown color of whole meal is attributable to polyphenol oxidase activity (Taranto et al. 2012, Mangini et al. 2014), but in case of CItr14629 the dark red color of anthocyanins probably overlapped and enhanced the brownish tone of quinone-derivatives arising from the enzymatic oxidation of phenolic compounds. Other, non-pigmented, samples also showed high BI values, in this case totally imputable to the oxidation of phenolics: they belonged to the ssp. dicoccoides and ssp. dicoccum, while the durum cultivars generally showed lower values. A significant correlation between BI and GPC was observed (R = 0.44, p < 0.05), already reported in previous papers (Taranto et al. 2012). Selection of the crosses The parental lines of the crosses have to be sufficiently differentiated for the trait of interest to ensure segrega- tion and subsequent mapping of each trait. The differences observed were markedly above LSD 0.05 in many of the examined crosses (Table 7). As expected, the highest differences in GPC were observed in the crosses involving cultivars and wild accessions or landraces, namely Latino x MG29896, Latino x MG5323, Svevo x MG4330, and Messapia x MG4343, which progeny can be effectively used for mapping this character. In particular, the recombi- nant inbred population originated by the cross Messapia x MG4343 has already been the object of a study aimed to detect GPC-related QTLs (Blanco et al. 2002). Similarly, the differences in DG were high in the crosses involving cultivars and wild accessions or landraces, as well as in some crosses involving cultivars, such as Neolatino x Preco (Preco, in particular, was a high-gluten cultivar) and Latinur x Saragolla. The differences in WG partly resembled those in dry gluten: apart Latino x MG29896 they were marked in the crosses involving cultivars and wild accessions or landraces and, again, in the cultivar crosses Neolatino x Preco and Latinur x Saragolla, but with the addition of the crosses Saragolla x 02-5B-318 (that showed the highest difference), and CItr 14629 x Grecale. *, **, *** indicate significance at p < 0.05, p < 0.01 and p < 0.001, respectively. A G R I C U LT U R A L A N D F O O D S C I E N C E A. Pasqualone et al. (2015) 24: 118–127 125 The highest DG/GPC differences were observed, in decreasing order, in Latinur x Saragolla, Isildur x Saragolla, and Liberdur x Saragolla (being Saragolla a cultivar with low DG and, therefore, low DG/GPC), as well as in Grecale x Fiore, Neolatino x Preco, and Duilio x AC Avonlea. Moreover, six of examined crosses showed the most differen- tiated values of GHI: Latino x Primadur, Svevo x MG4330, Latino x MG29896, Saragolla x 02-5B-318, CItr 14629 x Grecale, and Duilio x AC Avonlea. Table 7. Differences in grain protein content (GPC), wet gluten (WG), dry gluten (DG), gluten hydration index (GHI), dry gluten/protein ratio (DG/GPC), gluten index (GI), sodium dodecyl sulphate sedimentation volume (SDS-SV), thousand-kernel weight (KW), and color indices (YI = yellow index; RI = red index; BI = brown index) detected between parental lines of 28 wheat mapping populations Cross ΔGPC (% d.m.) ΔWG (% d.m.) ΔDG (% d.m.) ΔGHI ΔDG/ GPC ΔGI ΔSDS-SV (mL) ΔKW (g) ΔYI ΔRI ΔBI Aureo x Normanno 2.4 9.1 3.1 0.6 10.5 4 6 4.7 1.4 0.1 0.1 Aureo x Iride 2.6 7.1 2.8 1.5 6.9 1 18 2.1 0.7 0.1 0.4 Neolatino x Preco 1.8 16.5 4.6 1.5 18.8 31 8 4.5 3.6 0.3 1.0 Latinur x Saragolla 0.8 16.1 4.7 1.4 27.5 23 6 3.3 0.1 0.2 0.5 Isildur x Saragolla 0.2 11.5 3.2 0.0 24.2 8 9 0.6 2.4 0.5 0.4 Liberdur x Saragolla 0.3 9.9 3.3 0.2 24.1 7 8 1.3 1.8 0.3 0.3 Svevo x Primadur 1.2 1.0 0.1 1.4 5.0 21 13 20.6 3.8 0.5 0.0 UC1113 x Iride 2.1 9.5 3.4 1.0 12.2 1 5 3.6 0.4 0.1 0.2 Isildur x Anco Marzio 0.3 3.0 1.0 0.1 7.6 3 13 0.0 3.6 0.7 0.6 Liberdur x Anco Marzio 0.8 1.4 0.6 0.3 7.2 2 12 1.9 3.0 0.5 0.5 Normanno x Tiziana 0.6 9.3 2.6 1.1 14.9 15 5 1.5 2.1 0.1 0.8 Svevo x Messapia 1.5 5.6 1.6 0.2 3.6 3 6 3.6 1.0 0.2 0.1 Chinese Spring x Chinese Spring – 5A dic. 0.3 1.9 0.6 0.2 1.5 3 14 1.4 0.5 0.2 0.6 Iride x Fiore 0.2 4.9 1.3 0.9 8.3 4 1 7.3 1.1 0.1 0.8 Svevo x MG4330 3.1 18.7 4.5 4.4 12.8 19 10 13.6 0.9 1.1 2.3 Grecale x Tiziana 0.2 1.6 0.4 0.1 2.6 10 4 8.2 1.6 0.3 0.6 Iride x Ciccio 1.4 6.2 2.0 0.0 6.7 10 11 6.4 0.1 0.1 0.5 Normanno x Fiore 0.4 2.9 1.0 0.0 4.7 1 11 4.7 3.2 0.1 1.3 Duilio x AC Avonlea 0.4 13.6 3.0 2.9 17.2 42 4 13.2 3.0 0.7 0.5 CItr 14629 x Grecale 2.3 16.8 3.5 3.3 9.2 43 31 2.3 4.6 2.4 7.3 Grecale x Fiore 1.2 13.8 4.0 1.2 22.2 26 2 2.0 2.7 0.3 1.1 Latino x MG5323 3.6 15.0 4.3 0.1 9.2 2 4 23.6 0.9 1.6 4.0 Latino x Primadur 1.6 5.7 0.0 4.7 7.6 69 50 25.9 4.9 0.3 0.9 Svevo x Ciccio 1.0 0.9 0.4 0.3 2.1 13 1 4.6 0.6 0.2 0.3 Saragolla x 02-5B-318 2.3 25.3 6.4 3.6 9.7 45 14 11.4 4.8 1.0 0.0 Iride x Tiziana 0.8 7.3 2.3 0.2 11.3 12 7 1.1 0.0 0.1 0.3 Messapia x MG4343 3.4 n.d. n.d. n.d. n.d. n.d. 29 33.9 0.5 1.0 3.8 Latino x MG29896 4.6 10.2 4.9 4.0 7.4 58 36 12.4 2.2 0.6 1.5 LSD 0.05 1.2 5.4 1.5 1.2 7.7 10 9 4.6 0.9 0.4 0.9 Regarding GI, a relevant number of crosses showed marked differences. They included again Neolatino x Preco and Latinur x Saragolla - that could therefore be used for mapping both quantitative and qualitative features of gluten - but also many other crosses such as, in decreasing order, Latino x Primadur, Latino x MG29896, Saragolla x 02- 5B-318, CItr 14629 x Grecale, Dulio x AC Avonlea, and Grecale x Fiore, whose corresponding recombinant inbred populations can be considered potentially useful for mapping this essential qualitative characteristic. The differences in SDS-SV values were in accordance with those observed in GI, being these two indices correlat- ed. In particular, the most marked differences were observed, in decreasing order, in the crosses Latino x Primad- ur, Latino x MG29896, CItr 14629 x Grecale, and Messapia x MG4343. The recombinant inbred population orig- inated by the latter cross has already been effectively used to detect QTLs related to SDS-SV in a previous study (Blanco et al. 1998). A G R I C U LT U R A L A N D F O O D S C I E N C E A. Pasqualone et al. (2015) 24: 118–127 126 The most marked differences in KW were found in the crosses involving cultivars and wild accessions together, as well as in those involving Primadur, due to the generally low KW of this cultivar. In particular, the highest values were observed in Messapia x MG4343, Latino x Primadur, Latino x MG5323, and Svevo x Primadur. Regarding YI, the greatest differences between parental lines were observed for the crosses Latino x Primadur, Saragolla x 02-5B-318, and CItr 14629 x Grecale, that appeared all suitable for mapping yellow color. As far as RI is concerned, the cross CItr 14629 x Grecale, involving a purple wheat line and a conventional non-pigmented wheat cultivar, was by far the most suitable for mapping this parameter. A weaker difference in red tone was present in some crosses, such as Latino x MG5323 and Svevo x MG4330. Not imputable to anthocyanins, this difference probably originated from the oxidation of phenolic substances occurring at different extents. Only slight differences of BI were observed between parental lines when durum cultivars were involved, whereas more marked differences were observed in crosses involving ssp. dicoccoides or ssp. dicoccum. The highest differ- ence was found in the cross CItr14629 x Grecale, but probably the interference of anthocyanins could have over- estimated it. Hence, although being constituted by less differentiated parental lines, the crosses Latino x MG5323, Messapia x MG4343, and Svevo x MG4330 appeared more suitable for mapping BI. Conclusions Overall, many of the examined crosses appeared to be suitable for studies focused on mapping each of the main quality traits of wheat (GPC, quantity and quality of gluten, KW, color indices). 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Relationship between glutenin subunit composition and gluten strength measurements in durum wheat. Journal of the Science of Food and Agriculture 85: 2445–2452. Taranto, F., Delvecchio, L.N., Mangini, G., Del Faro, L., Blanco, A. & Pasqualone, A. 2012. Molecular and physic-chemical evalua- tion of enzymatic browning of whole meal and dough in a collection of tetraploid wheats. Journal of Cereal Science 55: 405–414. Quality characteristics of parental lines of wheat mappingpopulations Introduction Material and methods Samples Basic chemical and physical determinations Determination of quali-quantitative features of gluten Sedimentation volume in sodium dodecyl sulphate (SDS-SV) Electrophoretic analyses of seed storage proteins Statistical analyses Results and discussion Variability of quality characteristics and correlations among them Selection of the crosses Conclusions Acknowledgement References