A G R I C U LT U R A L A N D F O O D S C I E N C E A. Konieczny & I. Kowalska (2016) 25: 124–137 124 Manuscript received February 2016 The role of arbuscular mycorrhiza in zinc uptake by lettuce grown at two phosphorus levels in the substrate Anna Konieczny, Iwona Kowalska Unit of Plant Nutrition, Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, 29 Listopada 54, 31-425 Krakow, Poland e-mail: rokowals@cyf-kr.edu.pl Arbuscular mycorrhizal fungi (AMF) play an important role in Zn uptake by plants and can partially mitigate the effects of its deficit. On the other hand, they are involved in reducing the accumulation of Zn and its toxicity to plants when it is present in excessive concentrations in the soil. The aim of the study was to investigate the effect of two AMF, i.e., Funneliformis mosseae and Rhizophagus intraradices on Zn uptake by lettuce plants grown at two P levels and elevated concentrations of Zn in a peat substrate. The experiment demonstrated the effectiveness of mycorrhization of lettuce grown in the peat substrate; however, the arbuscular mycorrhiza did not reduce the uptake of Zn by let- tuce. The AMF used in the experiment differentially affected the Zn content in lettuce. Compared to uninoculated plants, R. intraradices increased the Zn content in lettuce, whereas F. mosseae did not affect the Zn content. Key words: heavy metal, Funneliformis mosseae, Rhizophagus intraradices, plant nutrition, yield Introduction Arbuscular mycorrhizal fungi (AMF) can form mutualistic relationships with at least 90% of plant species, includ- ing horticultural plants. This symbiosis, which occurs in plant roots, is based on the bidirectional exchange of sub- stances between the fungus and the host plant (Smith and Read 2008). The AMF increase the absorption surface of the roots and the bioavailability of some nutrients, which contributes to enhancing nutrient and water uptake by plants (Karandashov and Bucher 2005, Karagiannidis et al. 2007). Moreover, these fungi stimulate production of hormones that regulate plant growth and plant tolerance to biotic and abiotic stress factors, including an in- creased concentration of heavy metals in the rhizosphere (Li and Christie 2001, Vincente-Sánchez et al. 2014, Ruiz-Lozano et al. 2016). The effect of AMF in reducing the heavy metal content in plants might be a result of the strong metal-binding capacity of mycorrhizal structures, as well as due to the immobilisation of metal ions in the mycorrhizosphere (Joner et al. 2000, Christie et al. 2004). Furthermore, the increased plant tolerance to stress might be associated with a better plant nutritional status, the enhanced production of phytohormones, as well as an improvement in the structure of the substrate by the fungus, which leads to an improvement in plant growth and development in unfavorable environmental conditions (Turnau et al. 2002, Christie et al. 2004). The metal-binding capacity of AMF might be related to the amount of external mycelia and internal hyphae (Christie et al. 2004), which is influenced by many external factors (Smith and Read 2008). One of these factors is a high concentration of phosphorus (P) in the root zone, which causes a reduction in the development of mycorrhizal structures, which has been demonstrated by many studies (Schmidt et al. 2010, Kowalska et al. 2015). Because of its physiological function, zinc (Zn) is an essential micronutrient for plant growth and development (Benton Storey 2007). The general values for the average Zn content in soils all over the world range between 60 and 89 mg kg-1 (Kabata-Pendias 2011). However, Zn is a component of many waste substances and compounds released into the environment, which might cause its excessive accumulation in the soil, especially in industrialised areas. Increased Zn concentration in the soil mainly results of the emissions from smelters and incinerators, exces- sive application of Zn fertilizers and pesticides, using of contaminated sewage sludge, dispersal of main waste as well as release of Zn from galvanized wastes (Chaney 1993). Contemporarily observed soil contamination with Zn contributed to its extremely high accumulation in the top layer of soil in certain areas (Kabata-Pendias 2011). The Zn contents in soil found near mine and industrializes areas reached 180000 in Belgium, 50000 in United States and 13800 mg kg-1 in Poland (Rosen et al. 1978, Scokart et al. 1983, Kabata-Pendias 2011). An excessive concen- tration of Zn in the soil leads to an increased concentration of plants, where it affects plant growth and develop- ment and thereby reduces the quantity and quality of yield (Chaney 1993). Arbuscular mycorrhizal fungi play an important role in Zn uptake and can partly mitigate the effects of its deficit (Thompson et al. 2013). Surprisingly, AMF are involved in reducing the accumulation of Zn and its toxicity to plants in excessive concentrations of this heavy metal in the soil (Li and Christie 2001, Chen et al. 2003). A G R I C U LT U R A L A N D F O O D S C I E N C E A. Konieczny & I. Kowalska (2016) 25: 124–137 125 Lettuce (Lactuca sativa L.), is a popular vegetable in the human diet, due to its nutritional value and low calorific content (Nicolle et al. 2004). It belongs to the group of leafy vegetables that have the highest ability to accumu- late heavy metals (Smical et al. 2008). Therefore, by limiting the uptake and accumulation of Zn, AMF might sig- nificantly contribute to improving the nutritional value of this vegetable when it is grown in the presence of a high concentration of this heavy metal in the root zone. We hypothesized that mycorrhizal colonisation of lettuce roots grown in high Zn concentrations in the substrate reduces the content of Zn in lettuce and improves its nutritional quality. Therefore, the main objective of this study was to evaluate effect of AMF on Zn uptake by lettuce plants grown in elevated concentrations of this micronu- trient in the substrate. We also determined the effect of two P levels in the substrate on the colonisation of let- tuce roots with AMF, as well as effect of different P and Zn levels in the substrate, and the effect of colonisation of lettuce roots with AMF on the nutritional status of plants, and on the quantity and quality of the yield. Material and methods Biological material and experimental design A 2 × 3 × 3 factorial experiment was conducted in the spring of 2014 and 2015 at the foil tunnel belonging to the University of Agriculture in Krakow. Lettuce plants (Lactuca sativa L.) cv. Melodion (Enza Zaden) were grown in pots filled with peat substrate. The first factor of the experiment included two levels of P in the substrate (70 or 140 mg dm-3), the second consisted of three levels of Zn in the substrate (20, 50 or 100 mg dm-3) and the third consisted of inoculation of the peat substrate with arbuscular mycorrhizal fungi (–AMF - not inoculated, +AMF 1 - inoculated with Funneliformis mosseae, +AMF 2 - inoculated with Rhizophagus intraradices). The experiment con- sisted of 18 treatments, each in triplicate and each replication consisted of eight pots. Untreated lettuce seeds were sown in multipots (DP 42/96, 96 cone-shaped cells, 55 cm3 volume of single cell) filled with peat substrate, limed and supplemented with nutrients to the level recommended for the production of lettuce seedlings. At the time of sowing, some batches of seeds were treated with inoculum containing F. mos- seae or R. intraradices (SYMBIOM ®, 720 propagules g-1), by mixing the inoculum with the substrate. The seedlings were transferred to 2 – L pots filled with peat substrate when the seedlings had 3–4 fully developed leaves. The peat substrate was prepared using moss peat (Kronen®), limed on the basis of a neutralisation curve to pH 6.0. The content of nutrients for all plants, with the exception of P and Zn, was supplemented to the same level i.e., (in mg dm-3): N – 180, K – 220, Mg – 160, Fe – 20, Mn – 20, Cu – 5, B – 1 , Mo – 1. Plants were divided into two sub- blocks, which received one of two different concentrations of P (Ca[H 2 PO 4 ] 2 H 2 O): a concentration that was opti- mal for lettuce - 140 mg dm-3, or a lower concentration of 70 mg dm-3. In each sub-block, three different concen- trations of Zn (ZnSO 4˙ 7H 2 O) were applied: 20 (optimal for lettuce), 50 or 100 mg dm-3. Part of the peat substrate within each Zn concentration was inoculated with F. mosseae or R. intraradices (SYMBIOM ® , 720 propagules g-1), at a concentration of 5 g dm-3 of the substrate. The fertilizers and then the inoculum were separately introduced to the substrate before planting, by mixing with the substrate. Plants were irrigated solely with water throughout the growing season, which was applied by a drip irrigation system. The frequency of irrigation was adjusted to the phase of growth and substrate moisture. The temperature in the tunnel was maintained at 16 ˚C on cloudy days, 21 ˚C on sunny days and 12 ˚C at night. Lettuce plants were harvested once, at the consumption stage (46 days after planting seedlings into the pots) and then the plant material was analysed. Observation and measurements Mycorrhizal colonisation The root systems of the lettuce plants were isolated at the end of cultivation from three pots from each repli- cate. Samples of 10 g were collected from the isolated root systems and were used to prepare microscope slides according to a modified method of Phillips and Hayman (1970). The collected roots were cold-macerated in 10% KOH (12 h), rinsed with water, acidified with 5% lactic acid (12 h) and stained with 0.03% aniline blue dissolved a the mixture of lactic acid, glycerol and distilled water (v/v/v 1:1:1). The time of maceration and acidification was limited to 12 h due to the delicate structure of lettuce roots. Microscope slides were prepared from stained roots, cut into 1 cm pieces and encapsulated in a mixture of glycerol and lactic acid (v/v 5:1), and the slides were observed using an Axio Imager N2 (Carl Zeiss) microscope via Nomarsky optics. Each replication was represented by 90 pieces of root, each having a length of 1 cm. The colonisation of lettuce roots by AMF was assessed accord- A G R I C U LT U R A L A N D F O O D S C I E N C E A. Konieczny & I. Kowalska (2016) 25: 124–137 126 ing to the method of Trouvelot et al. (1986), by determining the following parameters: mycorrhizal frequency (F), relative mycorrhizal intensity (M), and relative abundance of arbuscules (A). These mycorrhizal parameters were calculated using Mycocalc software (http://www2.dijon.inra.fr/mychintec/Mycocalc-prg/download.html). Yield The yield of lettuce was expressed as the fresh weight of a head. Plants were collected separately from each replication, weighted, counted and the mean weight of a head was calculated for each replicate. Quality of plants At harvest, five representative plants from each replicate were selected for chemical analysis of the plant mate- rial. One-quarter of each lettuce head was chopped in a blender. Dry matter, using dryer method (105 ˚C) and the content of glucose, fructose and sucrose after ethanol extraction by capillary electrophoresis (Beckman Coulter PA 800plus Pharmaceutical Analysis System) using a kit from Analis Scientific Instruments for Laboratories (Bel- gium) (NaH 2 PO 4 (36 mmol), NaOH (130 mmol), β - cyclodextrin, pH = 12.7) were determined. A second quarter of each lettuce was cut with a porcelain knife and following extraction with 2% oxalic acid, the level of ascor- bic acid was determined by capillary electrophoresis using a buffer containing: NaH 2 PO 4 (30 mmol), Na 2 B 4 O 7 (15 mmol) and CTAB (0.2 mmol), pH = 8.80 (Zhao et al. 2011). The remaining half of each lettuce was dried at 65 ˚C (24 h) in a laboratory dryer with forced air circulation. Dried samples were ground in a variable speed rotor mill Pulverisette 14 (FRITSH) using a 0.05 mm sieve and the content of P, K, Ca, Mg, Cu, Fe, B, Mn, Mo and Zn was determined, after mineralisation in 65% extra pure HNO 3 in a CEM MARS-5 Xpress microwave system (Pasławski and Migaszewski 2006), using a high-dispersion spectrometer ICP-OES (Inductively Coupled Plasma Optical Emis- sion Spectrometry,Prodigy Teledyne Leeman Labs). The leaf N content was assayed by the Kjeldahl method using a VELP Scientifica UDK 193 distillation unit (Persson and Wennerholm 1999). Statistical analysis Statistical analyses were performed using STATISTICA 10.PL (StatSoft Inc., USA). A three-way analysis of variance was used to determine the main effects of experimental factors and interactions among main effects. To determine the significance between means, the HSD Tukey test was used. Tests were considered significant at a probability level below 0.05 (p < 0.05). Similar results were obtained in both years of the experiment and are therefore presented as the means of the data in 2014 and 2015. Results Mycorrhizal colonisation The effectiveness of mycorrhization of lettuce with both AMF i.e., F. mosseae (+AMF 1 ) and R. intraradices (+AMF 2 ) was demonstrated in this experiment. Independently of the other experimental factors, the mycorrhizal frequency (F) in roots of plants inoculated with F. mosseae and R. intraradices was 23.81 and 20.40%, respectively, and the relative mycorrhizal intensity (M) was 0.77 and 1.23%, respectively (Table 1). The relative abundance of arbuscules (A) in roots inoculated with R. intraradices was significantly higher than in F. mosseae and was 0.18 and 0.01%, respectively. The level of P in the substrate, irrespective of mycorrhiza, affected all the examined mycorrhizal parameters. Higher values of F, M and A were observed in the roots of plants grown in the substrate with a reduced concentration of P (70 mg dm-3) (Table 1). The roots of uninoculated plants showed no mycorrhizal structures. Yield The yield of lettuce, expressed as the weight of the head was influenced by all three experimental factors (Table 2). Taking the concentration of P into account, a significantly higher head weight was found at the standard P concen- tration, than at the reduced concentration (154.26 and 138.75 g, respectively). Increasing the content of Zn in the substrate led to a decrease in the lettuce head weight. However, there was no significant difference in the yield between plants grown in the substrate with 50 and 100 mg Zn. From the two AMF used in the experiment, only F. mosseae affected the lettuce yield. Plants inoculated with this fungus were characterised by a higher weight of head, compared to uninoculated plants and those inoculated with R. intraradices. A G R I C U LT U R A L A N D F O O D S C I E N C E A. Konieczny & I. Kowalska (2016) 25: 124–137 127 Table 1. Effect of the phosphorus (P) and zinc (Zn) level in the peat substrate and inoculation of the peat substrate with arbuscular mycorrhizal fungi on the mycorrhizal frequency (F), relative mycorrhizal intensity (M) and relative abundance of arbuscules (A) in lettuce roots P mg dm-3 Inoculation Zn mg dm-3 F(%) M(%) A(%) 70 –AMF 20 0.00±0.00 0.00±0.00 0.00±0.00 50 0.00±0.00 0.00±0.00 0.00±0.00 100 0.00±0.00 0.00±0.00 0.00±0.00 +AMF 1 20 37.04±6.49 9.57±0.34 0.00±0.00 50 35.66±3.51 1.30±0.81 0.03±0.03 100 35.18±2.06 1.37±0.65 0.01±0.01 +AMF 2 20 29.22±4.28 2.61±0.99 0.54±0.29 50 35.93±5.45 2.37±0.65 0.32±0.15 100 26.01±3.23 1.47±0.38 0.20±0.14 140 –AMF 20 0.00±0.00 0.00±0.00 0.00±0.00 50 0.00±0.00 0.00±0.00 0.00±0.00 100 0.00±0.00 0.00±0.00 0.00±0.00 +AMF 1 20 10.77±1.33 0.18±0.06 0.00±0.00 50 10.56±1.16 0.45±0.22 0.01±0.01 100 13.65±2.54 0.42±0.11 0.00±0.00 +AMF 2 20 10.09±1.95 0.31±0.08 0.01±0.01 50 13.02±1.92 0.49±0.20 0.00±0.00 100 8.12±1.59 0.14±0.04 0.01±0.00 Main effects 70 22.12±3.29 a 1.12±0.24 a 0.12±0.05 a 140 7.36±1.14 b 0.22±0.05 b 0.00±0.00 b –AMF 0.00±0.00 a 0.00±0.00 a 0.00±0.00 a +AMF 1 23.81±3.18 b 0.77±0.19 b 0.01±0.01 a +AMF 2 20.40±2.80 b 1.23±0.30 b 0.18±0.07 b 20 14.52±3.58 0.68±0.27 0.09±0.06 50 15.86±3.74 0.77±0.25 0.06±0.04 100 13.83±3.23 0.57±0.18 0.03±0.03 ANOVA P * * * Inoculation (AMF) * * * Zn n.s. n.s. n.s. P × AMF n.s. n.s. * P x Zn n.s. n.s. n.s. AMF x Zn n.s. n.s. n.s. P x AMF x Zn n.s. n.s. n.s. * = means are significantly different; n.s. = differences are not significant; ± = standard error of mean (SEM); a,b = means followed by different letters differ at p < 0.05; –AMF = uninoculated plants with arbuscular mycorrhizal fungi; +AMF 1 = plants inoculated with Funneliformis mosseae; +AMF 2 = plants inoculated with Rhizophagus intraradices A G R I C U LT U R A L A N D F O O D S C I E N C E A. Konieczny & I. Kowalska (2016) 25: 124–137 128 * = means are significantly different, n.s. = differences are not significant; ± = standard error of mean (SEM); a,b = means followed by different letters differ at p < 0.05; –AMF = uninoculated plants with arbuscular mycorrhizal fungi, +AMF 1 = plants inoculated with Funneliformis mosseae; +AMF 2 = plants inoculated with Rhizophagus intraradices Table 2. Effect of the phosphorus (P) and zinc (Zn) level in the peat substrate and inoculation of the peat substrate with AMF on the yield of lettuce expressed as the weight of a head P mg dm-3 Inoculation Zn mg dm-3 Yield g 70 –AMF 20 135.55±0.39 50 146.61±0.85 100 140.89±2.69 +AMF 1 20 150.51±0.27 50 133.25±2.64 100 139.23±2.40 +AMF 2 20 127.26±0.81 50 134.11±2.41 100 141.36±1.61 140 –AMF 20 157.79±6.11 50 145.12±3.73 100 145.38±0.43 +AMF 1 20 191.40±2.42 50 156.79±4.13 100 148.00±3.09 +AMF 2 20 155.38±9.75 50 143.98±5.06 100 144.52±4.37 Main effects 70 138.75±1.41 a 140 154.26± 3.10 b –AMF 145.22±1.96 a +AMF 1 153.20±4.63 b +AMF 2 141.10±2.73 a 20 152.98±5.20 b 50 143.31±2.26 a 100 143.23±1.18 a ANOVA P * Inoculation (AMF) * Zinc (Zn) * P × AMF * P x Zn * AMF x Zn * P x AMF x Zn n.s. A G R I C U LT U R A L A N D F O O D S C I E N C E A. Konieczny & I. Kowalska (2016) 25: 124–137 129 Quality of lettuce The quality of lettuce was evaluated by the content of dry matter, ascorbic acid, sugars (glucose, fructose, sucrose) as well as that of macro- and micronutrients. The dry matter content was influenced by the concentration of P in the substrate and inoculation with AMF (Table 3). Regardless of the other experimental factors, plants grown in the substrate with a reduced P concentra- tion were characterised by a higher dry matter content. The application of R. intraradices inoculum decreased the dry matter content in comparison to that of uninoculated plants and those inoculated with F. mosseae. Only the concentration of P in the substrate affected the content of ascorbic acid, which was higher at 70 mg dm-3 P than at 140 mg dm-3 P (Table 3). Table 3. Effect of the phosphorus (P) and zinc (Zn) level in the peat substrate and inoculation of the peat substrate with AMF on the content of dry matter, ascorbic acid, glucose and fructose in leaves of lettuce P mg dm-3 Inoculation Zn mg dm-3 Dry matter % Ascorbic acid Glucose Fructose mg 100 g-1 f.m. 70 –AMF 20 7.50±0.04 13.74±0.28 505.85±39.71 615.54±1.38 50 7.21±0.16 13.52±1.73 517.58±11.87 542.47±2.99 100 7.09±0.13 12.66±0.39 478.19±23.28 605.59±1.00 +AMF 1 20 7.80±0.32 14.72±0.58 635.89±44.31 690.43±67.74 50 7.84±0.11 16.04±0.15 602.94±83.14 635.57±17.38 100 7.31±0.10 14.09±0.71 491.63±45.24 543.57±51.55 +AMF 2 20 7.17±0.22 12.33±0.59 562.08±2.40 579.82±44.14 50 6.86±0.18 13.44±1.12 529.09±7.34 659.44±10.03 100 6.61±0.20 11.91±0.73 471.05±46.22 566.56±68.16 140 –AMF 20 6.49±0.07 11.09±0.57 518.51±3.20 537.83±25.35 50 6.86±0.13 10.58±0.29 522.52±20.40 687.69±3.98 100 6.75±0.13 11.40±2.42 584.48±13.35 704.26±27.49 +AMF 1 20 6.05±0.08 8.27±0.88 553.31±5.03 646.66±8.37 50 6.55±0.17 9.17±0.64 497.46±21.13 594.41±17.31 100 6.42±0.16 11.74±1.54 557.84±6.53 554.36±1.32 +AMF 2 20 5.94±0.13 12.77±0.66 516.06±4.61 597.50±3.68 50 6.01±0.04 9.65±0.26 520.03±22.51 594.19±1.45 100 6.82±0.23 9.67±1.12 485.28±23.68 528.45±58.42 Main effects 70 7.27±0.09 b 13.61±0.33 b 532.70±15.55 604.33±14.55 140 6.43±0.08 a 10.48±0.41 a 528.42±7.22 605.04±13.04 –AMF 6.98±0.09 b 12.16±0.52 521.19±10.77 615.56±16.39 +AMF 1 6.99±0.18 b 12.34±0.76 556.56±19.55 610.83±17.72 +AMF 2 6.56±0.13 a 11.63±0.45 513.93±10.76 587.66±17.02 20 6.83±0.18 12.15±0.55 548.62±13.58 611.30±16.76 50 6.89±0.14 12.06±0.67 531.65±15.21 618.96±12.19 100 6.83±0.09 11.91±0.56 511.41±14.79 583.80±20.72 ANOVA P * * n.s. n.s. Inoculation (AMF) * n.s. n.s. n.s. Zn n.s. n.s. n.s. n.s. P × AMF * n.s. n.s. n.s. P x Zn * n.s. n.s. n.s. AMF x Zn n.s. n.s. n.s. n.s. P x AMF x Zn n.s. n.s. n.s. n.s. * = means are significantly different, n.s. = differences are not significant; ± = standard error of mean (SEM); a,b = means followed by different letters differ at p < 0.05; –AMF = uninoculated plants with arbuscular mycorrhizal fungi; +AMF 1 = plants inoculated with Funneliformis mosseae, +AMF 2 = plants inoculated with Rhizophagus intraradices; f.m. = fresh matter A G R I C U LT U R A L A N D F O O D S C I E N C E A. Konieczny & I. Kowalska (2016) 25: 124–137 130 Among the sugars that were determined in the experiment (glucose, fructose, sucrose), the experimental fac- tors had a significant effect only on the content of sucrose. Significant interactions among all three experimental factors were observed on the content of sucrose (Fig. 1). The level of sucrose in uninoculated plants was slightly influenced by the P and Zn concentration in the substrate. The highest contents of sucrose were observed at the highest concentrations of Zn and P. However, a lower P concentration caused a marked increase in the accumula- tion of this sugar in inoculated plants (especially by F. mosseae). No effect of the Zn concentration on the content of sucrose in inoculated plants was observed. The effect of the experimental factors on the content of macro- and micronutrients in plants was variable. Regardless of the other experimental factors, the P concentration in the peat substrate affected the content of N, P and Ca (Table 4). A reduced P concentration in the substrate decreased the N and P content in lettuce, but led to an increase in the Ca level. Except for K, inoculation caused no significant differences in the content of macro- nutrients among inoculated and uninoculated plants. The content of K was higher in the leaves of mycorrhized plants. However, there were no significant differences between the effects of either species of AMF (Table 4). In- creasing the concentration of Zn in the substrate led to an increased content of Ca, K and Mg (Table 4). Higher concentrations of Ca and Mg were observed at 50 and 100 mg Zn, whereas an increased concentration of K was found only at 100 mg Zn. Regardless of the other experimental factors, the contents of Cu, Fe, and B were influenced by the P concentra- tion in the substrate (Table 5). However, these effects differed for different micronutrients. Higher contents of Cu and Fe were observed in lettuce grown in the substrate with a standard concentration of P, whereas for B, the opposite relationship was found. Inoculation with AMF affected the content of Cu and Mn (Table 5). The content of Cu in plants was higher in inoculated plants, irrespective of the species of AMF. Similarly, the Mn content was also higher in leaves of mycorrhized plants. However, despite this increase, the Mn content in lettuce mycorrhized with F. mosseae was similar to that in non-mycorrhized plants and those mycorrhized with R. intraradices. In general, an increasing concentration of Zn in the substrate caused an increase in the content of Fe and B (Table 5). A significant interaction among three experimental factors on the content of Zn was observed (Fig. 2). Regardless of the inoculation of the substrate with AMF, the content of Zn in lettuce increased with an increasing concen- tration of Zn in the substrate. At 20 and 50 mg of Zn in the substrate, the P concentration did not significantly af- fect the content of Zn in lettuce. However, at the highest level of Zn, i.e., 100 mg dm-3, in uninoculated plants, an increased content of Zn was observed at a higher concentration of P, whereas in inoculated plants, a higher concentration of P decreased the content of Zn in plants, which was particularly evident for R. intraradices. 0 50 100 150 200 250 300 350 400 450 500 20 mg Zn 50 mg Zn 100 mg Zn 20 mg Zn 50 mg Zn 100 mg Zn 20 mg Zn 50 mg Zn 100 mg Zn -AMF +AMF +AMF su cr os e (m g 10 0 g f.m ) 70 mg P dm 140 mg P dm -3 -3 -1 bc de ab cd a ab cdab cd e f cd e ef ab a bc d ab c cd e ab cd ab cd ab cd ab cd ab cd 1 2 Fig. 1. Interaction among the level of P, the level of Zn in the substrate and inoculation of the substrate with AMF on the content of sucrose in lettuce (mg 100 g-1 f. m.). –AMF = uninoculated plants, +AMF 1 = plants inoculated with Funneliformis mosseae, +AMF 2 = plants inoculated with Rizophagus intraradices; 20 mg Zn, 50 mg Zn, 100 mg Zn = 20, 50, 100 mg Zn dm-3; a,b,c, = means followed by different letters differ at p < 0.05; bars indicate standard error of mean (SEM); f.m. = fresh matter A G R I C U LT U R A L A N D F O O D S C I E N C E A. Konieczny & I. Kowalska (2016) 25: 124–137 131 Table 4. Effect of the phosphorus (P) and zinc (Zn) level in the peat substrate and inoculation of the peat substrate with AMF on the content of macronutrients (nitrogen [N], potassium [K], calcium [Ca], magnesium [Mg]) in leaves of lettuce P mg dm-3 Inoculation Zn mg dm-3 N P K Ca Mg % DM 70 –AMF 20 2.61±0.04 0.30±0.01 3.10±0.06 1.38±0.01 0.34±0.01 50 2.48±0.08 0.33±0.02 3.14±0.06 1.52±0.04 0.41±0.01 100 2.76±0.07 0.35±0.02 3.76±0.16 1.75±0.04 0.42±0.02 +AMF 1 20 2.50±0.08 0.31±0.01 3.28±0.15 1.32±0.06 0.30±0.01 50 2.33±0.06 0.32±0.01 3.16±0.04 1.51±0.12 0.36±0.03 100 2.57±0.09 0.35±0.01 3.82±0.14 1.75±0.03 0.40±0.01 +AMF 2 20 2.61±0.02 0.29±0.01 3.34±0.06 1.36±0.03 0.32±0.01 50 2.48±0.15 0.30±0.01 3.05±0.16 1.45±0.11 0.33±0.02 100 2.56±0.03 0.31±0.02 3.85±0.16 2.13±0.14 0.41±0.03 140 –AMF 20 2.75±0.08 0.45±0.02 3.07±0.20 1.27±0.08 0.36±0.03 50 2.89±0.03 0.47±0.03 3.29±0.23 1.53±0.13 0.40±0.04 100 2.55±0.08 0.47±0.01 3.24±0.09 1.77±0.03 0.39±0.01 +AMF 1 20 2.86±0.05 0.50±0.01 3.58±0.05 1.26±0.03 0.35±0.01 50 2.61±0.06 0.45±0.02 3.31±0.17 1.40±0.02 0.37±0.02 100 2.62±0.09 0.43±0.02 3.68±0.27 1.71±0.22 0.36±0.03 +AMF 2 20 2.62±0.03 0.45±0.03 3.42±0.05 1.27±0.06 0.34±0.01 50 2.67±0.01 0.49±0.02 3.73±0.05 1.52±0.10 0.39±0.04 100 2.63±0.04 0.43±0.01 3.38±0.04 1.55±0.05 0.37±0.01 Main effects 70 2.54±0.03 a 0.32±0.01 a 3.39±0.07 1.58±0.05 a 0.36±0.01 140 2.69±0.05 b 0.46±0.01 b 3.41±0.06 1.48±0.04 b 0.37±0.01 –AMF 2.67±0.04 0.40±0.02 3.27±0.08 a 1.54±0.05 0.39±0.01 +AMF 1 2.58±0.05 0.39±0.02 3.47±0.08 b 1.49±0.06 0.36±0.01 +AMF 2 2.60±0.03 0.38±0.02 3.46±0.07 b 1.55±0.07 0.36±0.01 20 2.66±0.03 0.38±0.02 3.30±0.05 a 1.31±0.02 a 0.34±0.01 a 50 2.58±0.05 0.39±0.02 3.28±0.07 a 1.49±0.03 b 0.38±0.01 b 100 2.61±0.03 0.39±0.02 3.62±0.08 b 1.55±0.06 c 0.39±0.01 b ANOVA P * * n.s. * n.s. Inoculation (AMF) n.s. n.s. * n.s. n.s. Zn n.s. n.s. * * * P × AMF n.s. n.s. n.s. n.s. n.s. P x Zn * * * n.s. * AMF x Zn n.s. n.s. n.s. n.s. n.s. P x AMF x Zn n.s. n.s. n.s. n.s. n.s. * = means are significantly different, n.s. = differences are not significant; ± = standard error of mean (SEM); a,b,c = means followed by different letters differ at p < 0.05; –AMF = uninoculated plants with arbuscular mycorrhizal fungi, +AMF 1 = plants inoculated with Funneliformis mosseae, +AMF 2 = plants inoculated with Rhizophagus intraradices; DM = dry matter A G R I C U LT U R A L A N D F O O D S C I E N C E A. Konieczny & I. Kowalska (2016) 25: 124–137 132 Besides, the significant interaction between the Zn concentration in the substrate and inoculation with AMF on the Zn content in lettuce (Fig. 3) showed that the increase of Zn content in lettuce was the greatest in plants grown at 100 mg Zn in the substrate and inoculated with R. intraradices. * = means are significantly different; n.s. = differences are not significant; ± = standard error of mean (SEM); a,b,c = means followed by different letters differ at p < 0.05; –AMF = uninoculated plants with arbuscular mycorrhizal fungi, +AMF 1 = plants inoculated with Funneliformis mosseae; +AMF 2 = plants inoculated with Rhizophagus intraradices; DM = dry matter Table 5. Effect of the phosphorus (P) and zinc (Zn) level in the peat substrate and inoculation of the peat substrate with AMF on the content of micronutrients (copper [Cu], iron [Fe], boron [B], manganese [Mg], molybdenum [Mo]) in leaves of lettuce P mg dm-3 Inoculation Zn mg dm-3 Cu Fe B Mn Mo mg kg-1 DM 70 –AMF 20 3.71±0.09 100.70±1.69 ab 25.37±0.70 186.85±6.46 0.25±0.02 50 2.74±0.05 107.60±6.13 abc 25.06±0.81 162.88±6.36 0.35±0.04 100 3.20±0.32 121.89±3.49 abc 28.44±0.32 174.47±2.08 0.28±0.06 +AMF 1 20 3.54±0.07 85.49±2.38 a 23.28±0.84 227.07±12.98 0.31±0.05 50 3.72±0.22 110.05±5.40 abc 25.42±1.69 167.30±24.75 0.33±0.09 100 4.13±0.38 141.26±2.09 abc 26.34±0.26 199.65±3.94 0.36±0.05 +AMF 2 20 3.88±0.19 96.88 ±1.91 ab 23.85±0.22 203.52±17.58 0.32±0.04 50 3.63±0.09 102.54±3.66 ab 23.92±1.54 164.62±15.48 0.21±0.01 100 3.67±0.38 129.59 ±10.54 abc 26.77±1.05 247.72±23.83 0.24±0.02 140 –AMF 20 4.13±0.10 108.18 ±5.11 abc 22.93±1.62 136.10±0.44 0.37±0.05 50 3.81±0.30 133.58 ±8.08 abc 26.07±1.89 207.62±27.94 0.28±0.03 100 4.09±0.12 133.59 ±2.41 abc 24.87±0.53 139.69± 14.53 0.31±0.10 +AMF 1 20 4.95±0.06 115.68 ±3.70 abc 23.11±0.39 158.94±13.61 0.19±0.01 50 4.12±0.60 113.16 ±.4.33 abc 23.88±0.31 211.43±26.04 0.23±0.03 100 4.33±0.25 116.07 ±8.03 abc 25.06±1.28 196.10±33.23 0.25±0.04 +AMF 2 20 4.42±0.35 113.38 ±5.67 abc 22.16±0.99 190.30±15.83 0.24±0.01 50 4.46±0.33 131.46 ±21.09 abc 24.32±0.96 336.04±77.22 0.43±0.10 100 4.38±0.29 130.80 ±2.76 abc 23.42±0.43 165.50±6.76 0.19±0.01 Main effects 70 3.58±0.10 a 110.67±3.50 a 25.38±0.40 b 192.68±6.87 0.29±0.02 140 4.30±0.10 b 121.77±3.07 b 23.98±0.37 a 193.53±14.25 0.28±0.02 –AMF 3.61±0.14 a 117.59±3.57 25.46±0.56 167.93±7.64 a 0.31±0.02 +AMF 1 4.13± 0.16 b 113.62±4.26 24.52±0.44 193.42±9.36 ab 0.28±0.02 +AMF 2 4.07±0.13 b 117.44±4.86 24.07±0.47 217.95±18.82 b 0.27±0.03 20 4.10±0.13 103.39±2.86 a 23.45±0.40 a 183.80±8.35 0.28±0.02 50 3.74±0.17 116.40±4.49 b 24.78±0.49 ab 208.31±19.46 0.30±0.03 100 3.97±0.11 128.86±2.81 c 25.82±0.46 b 187.19±10.23 0.27±0.02 ANOVA P * * * n.s. n.s. Inoculation (AMF) * n.s. n.s. * n.s. Zn n.s. * * n.s. n.s. P × AMF n.s. n.s. n.s. n.s n.s. P x Zn n.s. * n.s. n.s n.s. AMF x Zn n.s. n.s. n.s. n.s. n.s. P x AMF x Zn n.s. * n.s. n.s n.s. A G R I C U LT U R A L A N D F O O D S C I E N C E A. Konieczny & I. Kowalska (2016) 25: 124–137 133 Discussion The degree of the AMF colonisation of lettuce roots (23.81 and 20.40%, for +AMF 1 and +AMF 2 plants respective- ly) was lower than that described by Baslam et al. (2011), who cultivated lettuce in a mixture of vermiculite, sand and peat. Colonisation by AMF in their experiment ranged from 56 to 61% in roots of plants inoculated with a mixture of F. mosseae (G. mosseae) and R. intraradices (G. intraradices) and was about 65% in plants inoculated with R. fasciculatus (G. fasciculatum). The reason for this difference is probably connected with properties of used growing medium. Although, peat because of its physical and chemical properties seems to be suitable substrate for the development of AMF, the addition of mineral compounds could create better conditions for AMF devel- opment. Vestberg and Kukkonen (2007) proved that light Sphagnum peat clearly suppressed the function of AMF, resulting in low colonization level of plant roots. However, the functioning of AMF was improved in peat mixtures with clay or pumice. Linderman and Davis (2003) suggested that peats are not equal in physical, chemical and bio- logical properties, what can suppress or enhance mycorrhizal colonization. Additionally, these effects may also be connected with species of mycorrhizal fungus. We also suppose that cultivation period of lettuce in sterile grow- ing media, is too short for high development of mycorrhizal structures. Sterile growing media in contrast to soil are devoid of the presence of AMF and inoculum is introducing at the time of planting. 0 50 100 150 200 250 300 350 400 450 20 mg Zn 50 mg Zn 100 mg Zn 20 mg Zn 50 mg Zn 100 mg Zn 20 mg Zn 50 mg Zn 100 mg Zn -AMF +AMF +AMF Zn (m g kg D M ) 70 mg P dm 140 mg P dm -1 -3 a ab bc de a ab ef a ab c g a ab f a ab cd e a bc d de f 1 2 -3 Fig. 2. Interaction among the level of P, the level of Zn in the substrate and inoculation of the substrate with AMF on the content of Zn in lettuce (mg kg-1 DM). –AMF = uninoculated plants, +AMF 1 = plants inoculated with Funneliformis mosseae, +AMF 2 = plants inoculated with Rizophagus intraradices; 20 mg Zn, 50 mg Zn, 100 mg Zn = 20, 50, 100 mg Zn dm-3; a,b,c, = means followed by different letters differ at p < 0.05; bars indicate standard error of mean (SEM); DM = dry matter 0 50 100 150 200 250 300 350 400 450 -AMF +AMF +AMF Zn (m g kg D M ) 20 mg Zn dm 50 mg Zn dm 100 mg Zn dm -3 -3 a ab ab abcbc c d d e -1 1 2 -3 Fig. 3. Interaction between the level of Zn in the substrate and inoculation of the substrate with AMF on content of Zn in lettuce (mg kg-1 DM). –AMF = uninoculated plants; +AMF 1 = plants inoculated with Funneliformis mosseae; +AMF 2 = plants inoculated with Rhizophagus intraradices; a,b,c = means followed by different letters differ at p < 0.05; bars indicate standard error of mean (SEM); DM = dry matter A G R I C U LT U R A L A N D F O O D S C I E N C E A. Konieczny & I. Kowalska (2016) 25: 124–137 134 Despite the low mycorrhizal colonisation, an effect of mycorrhiza on the yield and some quality parameters was observed. Among the fungi used in the experiment, F. mosseae (+AMF 1 ) affected lettuce yield. Plants inoculated with this fungus showed the highest weight of a head. It is known that AMF lead to an increase in plant growth (Aguilera-Gomez et al. 1999). This beneficial effect is often associated with an enhancement of P uptake and bet- ter plant P status (Wu and Zou 2010). However, an increased P uptake by inoculated plants was not observed in this experiment, therefore, a higher weight of lettuce head was probably due to a different mechanism. According to Barea and Azcón-Aguilar (1982), mycorrhizal benefits might not be limited to improved nutrient uptake and the production of plant hormones by these mutualistic fungi might also be involved in their effects on plant metabolic processes. Funneliformis mosseae (G. mosseae) synthesised at least two gibberellin-like substances and four sub- stances with the properties of cytokinins (Barea and Azcón-Aguilar 1982). Allen et al. (1980) demonstrated that mycorrhizal infection increased the content of cytokinins in host plants. Since there was no effect of the mycor- rhiza on the P status of plants in this experiment, it is possible that a higher head weight of lettuces inoculated with F. mosseae might relate to the plant growth-regulating hormones discussed. Another possible explantation of higher weight of lettuce inoculated with F. mosseae may be connected with a higher amount of water in plant tissues (Marulanda et al. 2003). In our experiment at 70 mg of P and higher doses of Zn (50 and 100 mg) in substrate we observed the increased content of sucrose in plants inoculated with F. mosseae. The higher content of sucrose at lower P content in the substrate might be connected with slightly better development of mycorriza. The increase of the content of sugars in the presence of AMF agrees with the findings of Baslam et al. (2011), who observed an enhanced level of total soluble sugars in the inner leaves of two types of lettuce inoculated with a mixture of F. mosseae (G. mosseae) and R. intraradices (G. intraradices). This increased accumulation of total soluble sugars might be associated with an enhancement of photosynthesis in inoculated plants, since the beneficial effect of AMF on the photosynthetic rate has been demonstrated in several experiments (Wu and Zou 2010, Zhu et al. 2014). It can also be assumed that the increased rate of photosynthesis in plants inoculated with F. mosseae might also have increased the weight of lettuce heads in this experiment. The increase of these parameters was slight but statistically significant. Plant symbiosis with AMF can be influenced by many factors, including the P content in the root zone (Smith and Read 2008). It has been widely reported that a high concentration of P in the root zone reduces the colonisation of roots by AMF (Schmidt et al. 2010, Kowalska et al. 2015). The negative effect of a higher P concentration in the substrate (140 mg dm-3) on the development of mycorrhizal structures was also found in this experiment, com- pared with plants grown in conditions of P deficiency (70 mg dm-3). The reason for the limiting effect of high P doses on root colonisation by AMF is unclear. Amijee et al. (1989) cultivated leak plants at standard and elevated P concentrations and found large changes in the extension rate, infection delay and infection density, which might be due to the marked change in plant and fungal physiology. An important role of strigolactones as signaling molecules in the stimulation of spore germination and the development of extraradical mycelium has been suggested (Akiyama et al. 2005, Besserer et al. 2006). The exudation of these molecules by plant roots is promoted under conditions of P and N deficiency in the root zone. Optimal or increased concentrations of P and N reduce the exudation of strigolactones (Yoneyama et al. 2012), which might limit the growth of mycelium and decrease the probability of contact with the host plant. The scientific hypothesis of the present study was that AMF inoculation limits Zn uptake by the plants, when Zn concentration in the medium is high and close to toxic levels. However, no effect of AMF on reducing Zn uptake in lettuce grown in a high concentration of Zn in the substrate was demonstrated. Generally, regardless of inocula- tion with AMF, the Zn concentration in lettuce increased with an increasing concentration of Zn in the substrate. The highest Zn concentration was found in plants grown at 70 mg of P, 100 mg Zn and surprisingly, in plants that were inoculated with R. intraradices (Fig. 2). Kozik et. al (2009) also observed the increased Zn content in lettuce with increasing content of Zn in the substrate. In their experiment content of Zn in lettuce ranged between 128.6 and 291.1 mg kg-1 dry matter. In our experiment content of Zn in lettuce grown at optimal Zn dose in the sub- strate ranged between 44.90 and 63.50 mg kg-1 dry matter, which is in a range considered as optimal for lettuce i.e. 39–71 mg kg-1 dry matter (Benton Storey 2007). The contents of Zn in lettuce grown at 50 and 100 mg of Zn in the substrate were 96.06–139.26 and 161.18–358.12 mg kg-1 dry matter. Despite that, these values exceeded the optimal content of Zn in lettuce we did not observe the symptoms of its toxicity on plants. This suggests the high tolerance of lettuce to high Zn concentration in the root zone. It has been widely reported that inoculation with AMF enhances nutrient uptake by plants, including that of P and Zn (Thompson et al. 2013, Lehmann et al. 2014), but in conditions of high soil Zn concentration, AMF can reduce Zn accumulation in the aboveground parts of plant (Li and Christie 2001, Zhu et al. 2001). Zhu et al. (2001) found that increasing dose of Zn in the medium in a glasshouse pot experiment with white clover led to an increased A G R I C U LT U R A L A N D F O O D S C I E N C E A. Konieczny & I. Kowalska (2016) 25: 124–137 135 uptake of Zn, but this increase was greater in non-mycorrhizal than in mycorrhizal plants. A similar relationship was demonstrated by Li and Christie (2001) in red clover grown at four levels of Zn in the soil. In an experiment with tomato grown at two levels of Zn in the soil, i.e., low and high, Watts-Williams et al. (2013) found that myc- orrhizal plants accumulated more Zn when it was deficient in the soil (0.13 mg kg-1) and accumulated much less when the Zn content in the soil was at a toxic level (85.6 mg kg-1). The mechanism that limits the uptake, translocation and accumulation of Zn by AMF in plants grown in high and toxic Zn conditions is unclear, but is probably different from the mechanism which improves Zn uptake under conditions of Zn deficiency (Smith and Read 2008). The protective effect of AMF might result from Zn immobilisa- tion in the fungal mycelium (Chen et al. 2003), which possesses a very strong metal-binding capacity (Joner et al. 2000). This effect might also be associated with changes in Zn solubility, which is affected by the pH in the plant root zone (Li and Christie 2001). An improvement in plant P nutrition by AMF, which allows plants to alleviate the negative effect of Zn (Shetty et al. 1995), might also be involved. As mentioned above, the limiting effect of AMF on Zn uptake by lettuce was not observed in the present experi- ment; it is possible that the Zn dose used was too low to activate the mechanism that limits Zn uptake by inocu- lated plants. Moreover, the highest dose cannot be regarded as toxic, since no typical Zn toxicity symptoms on plants were observed, such as leaf chlorosis, browning or drying of the leaf blade or the stunting and dying of plants (Sagardoy et al. 2009). Increased doses of Zn slightly but significantly decreased the yield of lettuce, although the yield of plants grown on 50 or 100 mg of Zn substrate was similar. This weak effect of Zn dose on the yield of lettuce was independent of inoculation by AMF, confirming that even the highest dose of Zn was not detrimental to the plants. We expected that the highest dose would reduce the yield in –AMF plants and induce the protective mechanism of the AMF in +AMF plants, but this was not the case. Chen et al. (2003) demonstrated that in the presence of AMF, a critical Zn concentration exists, below which uptake of Zn was enhanced, whereas above this level, Zn translocation to the shoots decreased. Authors in their experiment with red clover grown at four Zn addition levels in soil (0, 50, 100 and 300 mg kg-1) found that, compared to un inoculated plants, AMF increased Zn content in plants grown at 0 and 50 mg and decreased at 100 and 300 mg of Zn. According to Christie et al. (2004), this critical value might differ between different soil types and plant species. Kozik et al. (2009) in experiment with lettuce grown at four Zn levels in peat substrate i.e. 10, 20,30, 60 mg dm-3 found the decrease of the yield with increasing Zn content, however the differences were not statistically significant. Authors did not observe the symptoms of Zn toxicity on plants. In our experiment, the symptoms of Zn toxicity were also not observed, but 50 and 100 mg doses of Zn caused slight but significant reduction of the yield. A lower Zn concentration in lettuce leaves was observed in plants inoculated with F. mosseae than in plants inoculated with R. intraradices, both grown at 70 mg of P and 100 mg of Zn in the dm-3 of substrate. Lingua et al. (2008) demonstrated a differential response of two poplar varieties on a high soil Zn concentration when the plants were inoculated with F. mosseae (G. mosseae) or R. intraradices (G. intraradices). Funneliformis mosseae (G. mosseae) slightly, but significantly, reduced the Zn accumulation in the leaves of one poplar variety, compared to uninoculated plants, whereas R. intraradices (G. intraradices) did not affect the Zn content of poplar plants. Therefore, AMF can alleviate the stress induced by high Zn concentrations in the plant root zone, but this response depends upon both the fungus and plant species. Generally, a beneficial effect of AMF on the uptake of some nutrients, i.e., K, Cu, Mn and Zn was observed in this experiment. It is known that by increasing the absorption surface of plant root system, AMF colonisation enhanc- es the uptake of nutrients (Smith and Read 2008). This effect was observed in this experiment, despite a low rel- ative abundance of arbuscules, which was 0.01 and 0.18%, for +AMF 1 and +AMF 2 , respectively. Arbuscules are bushy branched ends of AMF hyphae, which are located within plant root cells and are involved in the exchange of metabolites between a plant and the fungus (Parniske 2008). It is difficult to explain why AMF colonisation improved the plant nutritional status in K, Cu, Mn and Zn, despite the low relative abundance of arbuscules obtained in our experiment. Both R. intraradices and F. mosseae affected the content K, Cu and Mn to a similar extent. However, only R. intraradices increased the Zn content in lettuce. The reason for these differences between both fungi in Zn uptake by plants remains unclear. Limited Zn uptake in plants inoculated with F. mosseae might be linked with the protective effect of this fungus. Further studies are needed to estimate the critical concentration below which Zn uptake is enhanced, or above which it is limited, considering the characteristics of particular fungi. Moreover, the effect of AMF on nutritional status and metabolism of lettuce may also be affected by a cultivar of lettuce as well as the season of cultivation (Baslam et al. 2013). A G R I C U LT U R A L A N D F O O D S C I E N C E A. Konieczny & I. Kowalska (2016) 25: 124–137 136 The uptake of Zn by plants can also be influenced by the interaction of Zn with other nutrients in the soil, espe- cially P (Christie et al. 2004, Benton Storey 2007). Moreover, this interaction is even more complicated when the effect of AMF is superimposed (Li and Christie 2001). Shetty et al. (1995) assumed that P and Zn are mutually antagonistic when either element exceeds a threshold value. In our experiment, we found no interaction between the P and Zn concentration on their uptake by plants. The P level in the substrate did not affect the content of Zn in lettuce and moreover, the Zn level in the substrate had no effect on the P content of lettuce. This might be linked with the Zn dose used in the experiment, which was not high enough to reveal the antagonism between P and Zn or with the effect of the peat sorption complex on reducing contact between these two nutrients. In conclusion, no effect of AMF on the reduction of Zn uptake in lettuce grown at a high concentration of Zn in the substrate was demonstrated. The reason is probably that the Zn dose was too low to be toxic to lettuce and did not activate the mechanism that limits Zn uptake by inoculated plants. Moreover, two AMF differentially af- fected the Zn content in lettuce. 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