id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
afs-5942	Martín, Carmen; González-Benito, M. Elena	Cryopreservation and genetic stability of Dendranthema grandiflora Tzvelev in vitro cultures	2009	7	.pdf	application/pdf	4392	219	52	Sakai et al. (2000) reported a new encapsulation-dehydration method, tested not only with chrysanthemum shoot apices but also in wasabi and mint, that resulted in similar or better recovery than the other two methods tested (encapsulation-dehydration and vitrification), but made the protocol shorter and less handling was re- quired. A simple method based on sucrose pretreat- ment (3 days on 0.55 M sucrose, incubation for 1 h in 7.5% DMSO and direct immersion in liquid nitrogen) resulted in 60−75% shoot recovery in C. cinerariaefolium (Hitmi et al. 1999a, 2000a).	cache/afs-5942.pdf	txt/afs-5942.txt