id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
afs-72695	Jenes, Barnabas; Puolimatka, Matti; Bittencourt, Pedro; Pulli, Seppo	Time saving method for protoplast isolation, transformation and transient gene expression assay in barley	1994	7	.pdf	application/pdf	3307	183	53	It can therefore be a limiting factor in applying barley protoplasts to transient gene ex- pression studies. Further refinary of this method to isolate barley protoplasts directly from mature embryo-scutellum complexes is need- ed to improve the yield of protoplasts and the induction level of cell divisions.	cache/afs-72695.pdf	txt/afs-72695.txt