id	sid	tid	token	lemma	pos
afs-72416	1	1	maataloustieteellinen	maataloustieteellinen	VERB
afs-72416	1	2	aikakauskirja	aikakauskirja	PROPN
afs-72416	1	3	vol	vol	NOUN
afs-72416	1	4	.	.	PUNCT
afs-72416	2	1	63	63	NUM
afs-72416	2	2	:	:	PUNCT
afs-72416	2	3	371—378	371—378	NUM
afs-72416	2	4	,	,	PUNCT
afs-72416	2	5	1991	1991	NUM
afs-72416	2	6	protoplast	protoplast	NOUN
afs-72416	2	7	culture	culture	NOUN
afs-72416	2	8	and	and	CCONJ
afs-72416	2	9	plant	plant	NOUN
afs-72416	2	10	regeneration	regeneration	NOUN
afs-72416	2	11	of	of	ADP
afs-72416	2	12	different	different	ADJ
afs-72416	2	13	agronomically	agronomically	ADV
afs-72416	2	14	important	important	ADJ
afs-72416	2	15	brassica	brassica	NOUN
afs-72416	2	16	species	specie	NOUN
afs-72416	2	17	and	and	CCONJ
afs-72416	2	18	varieties	variety	NOUN
afs-72416	2	19	janos	janos	PROPN
afs-72416	2	20	pauk	pauk	PROPN
afs-72416	2	21	*	*	PROPN
afs-72416	2	22	,	,	PUNCT
afs-72416	2	23	sandor	sandor	PROPN
afs-72416	2	24	fekete	fekete	PROPN
afs-72416	2	25	*	*	PROPN
afs-72416	2	26	,	,	PUNCT
afs-72416	2	27	juha	juha	PROPN
afs-72416	2	28	vilkki	vilkki	PROPN
afs-72416	2	29	*	*	PUNCT
afs-72416	2	30	*	*	PUNCT
afs-72416	2	31	and	and	CCONJ
afs-72416	2	32	seppo	seppo	ADJ
afs-72416	2	33	pulli	pulli	PROPN
afs-72416	2	34	*	*	PUNCT
afs-72416	2	35	*	*	PUNCT
afs-72416	2	36	*	*	PUNCT
afs-72416	2	37	cereal	cereal	PROPN
afs-72416	2	38	research	research	PROPN
afs-72416	2	39	institute	institute	PROPN
afs-72416	2	40	,	,	PUNCT
afs-72416	2	41	pob	pob	PROPN
afs-72416	2	42	.	.	PROPN
afs-72416	2	43	391	391	NUM
afs-72416	2	44	,	,	PUNCT
afs-72416	2	45	h-6701	h-6701	PROPN
afs-72416	2	46	szeged	szeged	PROPN
afs-72416	2	47	,	,	PUNCT
afs-72416	2	48	hungary	hungary	PROPN
afs-72416	2	49	*	*	PUNCT
afs-72416	2	50	*	*	PUNCT
afs-72416	2	51	agricultural	agricultural	ADJ
afs-72416	2	52	research	research	NOUN
afs-72416	2	53	centre	centre	NOUN
afs-72416	2	54	,	,	PUNCT
afs-72416	2	55	institute	institute	NOUN
afs-72416	2	56	of	of	ADP
afs-72416	2	57	plant	plant	NOUN
afs-72416	2	58	breeding	breeding	NOUN
afs-72416	2	59	,	,	PUNCT
afs-72416	2	60	sf-31600	sf-31600	PROPN
afs-72416	2	61	jokioinen	jokioinen	PROPN
afs-72416	2	62	,	,	PUNCT
afs-72416	2	63	finland	finland	PROPN
afs-72416	2	64	abstract	abstract	NOUN
afs-72416	2	65	.	.	PUNCT
afs-72416	3	1	protoplast	protoplast	NOUN
afs-72416	3	2	cultures	culture	NOUN
afs-72416	3	3	were	be	AUX
afs-72416	3	4	prepared	prepare	VERB
afs-72416	3	5	from	from	ADP
afs-72416	3	6	6	6	NUM
afs-72416	3	7	-	-	PUNCT
afs-72416	3	8	day	day	NOUN
afs-72416	3	9	-	-	PUNCT
afs-72416	3	10	old	old	ADJ
afs-72416	3	11	hypocotyls	hypocotyls	NOUN
afs-72416	3	12	of	of	ADP
afs-72416	3	13	six	six	NUM
afs-72416	3	14	spring	spring	NOUN
afs-72416	3	15	,	,	PUNCT
afs-72416	3	16	seven	seven	NUM
afs-72416	3	17	winter	winter	NOUN
afs-72416	3	18	cultivars	cultivar	NOUN
afs-72416	3	19	of	of	ADP
afs-72416	3	20	brassica	brassica	PROPN
afs-72416	3	21	napus	napus	PROPN
afs-72416	3	22	l.	l.	PROPN
afs-72416	3	23	and	and	CCONJ
afs-72416	3	24	one	one	NUM
afs-72416	3	25	line	line	NOUN
afs-72416	3	26	of	of	ADP
afs-72416	3	27	brassica	brassica	PROPN
afs-72416	3	28	campeslris	campeslris	PROPN
afs-72416	3	29	l.	l.	PROPN
afs-72416	3	30	the	the	DET
afs-72416	3	31	molarity	molarity	NOUN
afs-72416	3	32	of	of	ADP
afs-72416	3	33	enzyme	enzyme	NOUN
afs-72416	3	34	solution	solution	NOUN
afs-72416	3	35	was	be	AUX
afs-72416	3	36	raised	raise	VERB
afs-72416	3	37	to	to	ADP
afs-72416	3	38	0,714	0,714	NUM
afs-72416	3	39	m	m	NOUN
afs-72416	3	40	mannitol	mannitol	NOUN
afs-72416	3	41	resulting	result	VERB
afs-72416	3	42	in	in	ADP
afs-72416	3	43	well	well	ADV
afs-72416	3	44	manipulable	manipulable	ADJ
afs-72416	3	45	,	,	PUNCT
afs-72416	3	46	cytoplasm	cytoplasm	ADJ
afs-72416	3	47	dense	dense	ADJ
afs-72416	3	48	protoplasts	protoplast	NOUN
afs-72416	3	49	.	.	PUNCT
afs-72416	4	1	in	in	ADP
afs-72416	4	2	the	the	DET
afs-72416	4	3	protoplast	protoplast	NOUN
afs-72416	4	4	purification	purification	NOUN
afs-72416	4	5	procedure	procedure	NOUN
afs-72416	4	6	densitygradient	densitygradient	NOUN
afs-72416	4	7	centrifugationwas	centrifugationwas	PROPN
afs-72416	4	8	used	use	VERB
afs-72416	4	9	to	to	PART
afs-72416	4	10	minimize	minimize	VERB
afs-72416	4	11	physical	physical	ADJ
afs-72416	4	12	damage	damage	NOUN
afs-72416	4	13	of	of	ADP
afs-72416	4	14	protoplasts	protoplast	NOUN
afs-72416	4	15	.	.	PUNCT
afs-72416	5	1	three	three	NUM
afs-72416	5	2	different	different	ADJ
afs-72416	5	3	protoplast	protoplast	NOUN
afs-72416	5	4	culture	culture	NOUN
afs-72416	5	5	systems	system	NOUN
afs-72416	5	6	—	—	PUNCT
afs-72416	5	7	(	(	PUNCT
afs-72416	5	8	1	1	X
afs-72416	5	9	)	)	PUNCT
afs-72416	5	10	liquid	liquid	NOUN
afs-72416	5	11	,	,	PUNCT
afs-72416	5	12	(	(	PUNCT
afs-72416	5	13	2	2	NUM
afs-72416	5	14	)	)	PUNCT
afs-72416	5	15	2nd	2nd	ADJ
afs-72416	5	16	day	day	NOUN
afs-72416	5	17	embedded	embed	VERB
afs-72416	5	18	,	,	PUNCT
afs-72416	5	19	(	(	PUNCT
afs-72416	5	20	3	3	X
afs-72416	5	21	)	)	PUNCT
afs-72416	5	22	directly	directly	ADV
afs-72416	5	23	embedded	embed	VERB
afs-72416	5	24	in	in	ADP
afs-72416	5	25	low	low	ADJ
afs-72416	5	26	melting	melting	NOUN
afs-72416	5	27	agarose	agarose	NOUN
afs-72416	5	28	were	be	AUX
afs-72416	5	29	compared	compare	VERB
afs-72416	5	30	.	.	PUNCT
afs-72416	6	1	the	the	DET
afs-72416	6	2	two	two	NUM
afs-72416	6	3	different	different	ADJ
afs-72416	6	4	protoplast	protoplast	NOUN
afs-72416	6	5	embedding	embed	VERB
afs-72416	6	6	techniques	technique	NOUN
afs-72416	6	7	resulted	result	VERB
afs-72416	6	8	in	in	ADP
afs-72416	6	9	the	the	DET
afs-72416	6	10	same	same	ADJ
afs-72416	6	11	efficiency	efficiency	NOUN
afs-72416	6	12	of	of	ADP
afs-72416	6	13	cell	cell	NOUN
afs-72416	6	14	division	division	NOUN
afs-72416	6	15	as	as	ADP
afs-72416	6	16	the	the	DET
afs-72416	6	17	liquid	liquid	ADJ
afs-72416	6	18	culture	culture	NOUN
afs-72416	6	19	method	method	NOUN
afs-72416	6	20	and	and	CCONJ
afs-72416	6	21	over	over	ADP
afs-72416	6	22	this	this	DET
afs-72416	6	23	fact	fact	NOUN
afs-72416	6	24	the	the	DET
afs-72416	6	25	colony	colony	NOUN
afs-72416	6	26	browning	browning	NOUN
afs-72416	6	27	was	be	AUX
afs-72416	6	28	avoided	avoid	VERB
afs-72416	6	29	.	.	PUNCT
afs-72416	7	1	using	use	VERB
afs-72416	7	2	protoplast	protoplast	NOUN
afs-72416	7	3	agarose	agarose	NOUN
afs-72416	7	4	-	-	PUNCT
afs-72416	7	5	embedding	embed	VERB
afs-72416	7	6	and	and	CCONJ
afs-72416	7	7	culture	culture	NOUN
afs-72416	7	8	techniques	technique	NOUN
afs-72416	7	9	,	,	PUNCT
afs-72416	7	10	healthy	healthy	ADJ
afs-72416	7	11	calli	calli	NOUN
afs-72416	7	12	were	be	AUX
afs-72416	7	13	obtained	obtain	VERB
afs-72416	7	14	for	for	ADP
afs-72416	7	15	plant	plant	NOUN
afs-72416	7	16	regeneration	regeneration	NOUN
afs-72416	7	17	experiments	experiment	NOUN
afs-72416	7	18	.	.	PUNCT
afs-72416	8	1	incorporation	incorporation	NOUN
afs-72416	8	2	of	of	ADP
afs-72416	8	3	silver	silver	ADJ
afs-72416	8	4	nitrate	nitrate	NOUN
afs-72416	8	5	into	into	ADP
afs-72416	8	6	the	the	DET
afs-72416	8	7	regeneration	regeneration	NOUN
afs-72416	8	8	medium	medium	NOUN
afs-72416	8	9	improved	improve	VERB
afs-72416	8	10	the	the	DET
afs-72416	8	11	efficiency	efficiency	NOUN
afs-72416	8	12	of	of	ADP
afs-72416	8	13	plant	plant	NOUN
afs-72416	8	14	regeneration	regeneration	NOUN
afs-72416	8	15	in	in	ADP
afs-72416	8	16	responsive	responsive	ADJ
afs-72416	8	17	genotypes	genotype	NOUN
afs-72416	8	18	and	and	CCONJ
afs-72416	8	19	the	the	DET
afs-72416	8	20	regeneration	regeneration	NOUN
afs-72416	8	21	was	be	AUX
afs-72416	8	22	induced	induce	VERB
afs-72416	8	23	in	in	ADP
afs-72416	8	24	three	three	NUM
afs-72416	8	25	nonresponsive	nonresponsive	ADJ
afs-72416	8	26	(	(	PUNCT
afs-72416	8	27	without	without	ADP
afs-72416	8	28	silver	silver	ADJ
afs-72416	8	29	nitrate	nitrate	NOUN
afs-72416	8	30	)	)	PUNCT
afs-72416	8	31	genotypes	genotype	NOUN
afs-72416	8	32	,	,	PUNCT
afs-72416	8	33	too	too	ADV
afs-72416	8	34	.	.	PUNCT
afs-72416	9	1	the	the	DET
afs-72416	9	2	supplement	supplement	NOUN
afs-72416	9	3	of	of	ADP
afs-72416	9	4	silver	silver	ADJ
afs-72416	9	5	nitrate	nitrate	NOUN
afs-72416	9	6	in	in	ADP
afs-72416	9	7	regeneration	regeneration	NOUN
afs-72416	9	8	medium	medium	NOUN
afs-72416	9	9	was	be	AUX
afs-72416	9	10	especially	especially	ADV
afs-72416	9	11	advantageous	advantageous	ADJ
afs-72416	9	12	in	in	ADP
afs-72416	9	13	plant	plant	NOUN
afs-72416	9	14	regeneration	regeneration	NOUN
afs-72416	9	15	of	of	ADP
afs-72416	9	16	b.	b.	PROPN
afs-72416	9	17	campeslris	campeslris	PROPN
afs-72416	9	18	.	.	PUNCT
afs-72416	10	1	out	out	ADP
afs-72416	10	2	of	of	ADP
afs-72416	10	3	fourteen	fourteen	NUM
afs-72416	10	4	commercial	commercial	ADJ
afs-72416	10	5	cultivars	cultivar	NOUN
afs-72416	10	6	of	of	ADP
afs-72416	10	7	brassica	brassica	PROPN
afs-72416	10	8	napus	napus	PROPN
afs-72416	10	9	and	and	CCONJ
afs-72416	10	10	b.	b.	PROPN
afs-72416	10	11	campeslris	campeslris	PROPN
afs-72416	10	12	,	,	PUNCT
afs-72416	10	13	there	there	PRON
afs-72416	10	14	is	be	VERB
afs-72416	10	15	only	only	ADV
afs-72416	10	16	one	one	NUM
afs-72416	10	17	recalcitrant	recalcitrant	ADJ
afs-72416	10	18	genotype	genotype	NOUN
afs-72416	10	19	in	in	ADP
afs-72416	10	20	obtaining	obtain	VERB
afs-72416	10	21	plantlets	plantlet	NOUN
afs-72416	10	22	from	from	ADP
afs-72416	10	23	protoplast	protoplast	NOUN
afs-72416	10	24	-	-	PUNCT
afs-72416	10	25	derived	derive	VERB
afs-72416	10	26	calli	calli	NOUN
afs-72416	10	27	.	.	PUNCT
afs-72416	11	1	index	index	NOUN
afs-72416	11	2	words	word	NOUN
afs-72416	11	3	:	:	PUNCT
afs-72416	11	4	protoplast	protoplast	NOUN
afs-72416	11	5	isolation	isolation	NOUN
afs-72416	11	6	,	,	PUNCT
afs-72416	11	7	protoplast	protoplast	NOUN
afs-72416	11	8	culture	culture	NOUN
afs-72416	11	9	,	,	PUNCT
afs-72416	11	10	silver	silver	NOUN
afs-72416	11	11	nitrate	nitrate	NOUN
afs-72416	11	12	,	,	PUNCT
afs-72416	11	13	plant	plant	NOUN
afs-72416	11	14	regeneration	regeneration	NOUN
afs-72416	11	15	,	,	PUNCT
afs-72416	11	16	brassica	brassica	NOUN
afs-72416	11	17	napus	napus	PROPN
afs-72416	11	18	l	l	PROPN
afs-72416	11	19	,	,	PUNCT
afs-72416	11	20	brassica	brassica	PROPN
afs-72416	11	21	campeslris	campeslris	PROPN
afs-72416	11	22	l.	l.	PROPN
afs-72416	11	23	introduction	introduction	NOUN
afs-72416	11	24	since	since	SCONJ
afs-72416	11	25	several	several	ADJ
afs-72416	11	26	of	of	ADP
afs-72416	11	27	the	the	DET
afs-72416	11	28	most	most	ADV
afs-72416	11	29	widespread	widespread	ADJ
afs-72416	11	30	methods	method	NOUN
afs-72416	11	31	of	of	ADP
afs-72416	11	32	introducing	introduce	VERB
afs-72416	11	33	foreign	foreign	ADJ
afs-72416	11	34	genes	gene	NOUN
afs-72416	11	35	into	into	ADP
afs-72416	11	36	plant	plant	NOUN
afs-72416	11	37	cells	cell	NOUN
afs-72416	11	38	rely	rely	VERB
afs-72416	11	39	on	on	ADP
afs-72416	11	40	protoplasts	protoplast	NOUN
afs-72416	11	41	,	,	PUNCT
afs-72416	11	42	the	the	DET
afs-72416	11	43	establishment	establishment	NOUN
afs-72416	11	44	of	of	ADP
afs-72416	11	45	a	a	DET
afs-72416	11	46	reproducible	reproducible	ADJ
afs-72416	11	47	protoplast	protoplast	NOUN
afs-72416	11	48	-	-	PUNCT
afs-72416	11	49	plant	plant	NOUN
afs-72416	11	50	system	system	NOUN
afs-72416	11	51	is	be	AUX
afs-72416	11	52	of	of	ADP
afs-72416	11	53	a	a	DET
afs-72416	11	54	crucial	crucial	ADJ
afs-72416	11	55	importance	importance	NOUN
afs-72416	11	56	in	in	ADP
afs-72416	11	57	any	any	DET
afs-72416	11	58	plant	plant	NOUN
afs-72416	11	59	species	specie	NOUN
afs-72416	11	60	of	of	ADP
afs-72416	11	61	biotechnological	biotechnological	ADJ
afs-72416	11	62	interest	interest	NOUN
afs-72416	11	63	.	.	PUNCT
afs-72416	12	1	the	the	DET
afs-72416	12	2	first	first	ADJ
afs-72416	12	3	successful	successful	ADJ
afs-72416	12	4	!	!	PUNCT
afs-72416	13	1	protoplast	protoplast	NOUN
afs-72416	13	2	isolationand	isolationand	ADP
afs-72416	13	3	plant	plant	NOUN
afs-72416	13	4	regeneration	regeneration	NOUN
afs-72416	13	5	experiments	experiment	NOUN
afs-72416	13	6	have	have	AUX
afs-72416	13	7	been	be	AUX
afs-72416	13	8	performed	perform	VERB
afs-72416	13	9	by	by	ADP
afs-72416	13	10	wenzel	wenzel	PROPN
afs-72416	13	11	(	(	PUNCT
afs-72416	13	12	1973	1973	NUM
afs-72416	13	13	)	)	PUNCT
afs-72416	13	14	.	.	PUNCT
afs-72416	14	1	the	the	DET
afs-72416	14	2	brassica	brassica	PROPN
afs-72416	14	3	species	specie	NOUN
afs-72416	14	4	vary	vary	VERB
afs-72416	14	5	considerably	considerably	ADV
afs-72416	14	6	in	in	ADP
afs-72416	14	7	their	their	PRON
afs-72416	14	8	ability	ability	NOUN
afs-72416	14	9	to	to	ADP
afs-72416	14	10	371	371	NUM
afs-72416	14	11	journal	journal	NOUN
afs-72416	14	12	of	of	ADP
afs-72416	14	13	agricultural	agricultural	ADJ
afs-72416	14	14	science	science	NOUN
afs-72416	14	15	in	in	ADP
afs-72416	14	16	finland	finland	PROPN
afs-72416	14	17	https://www.c-info.fi/en/info/?token=u8o8ifesqglzteib.c-zfi2k_0f23pzduelcv3a.48tnvgoaiagujk7z6ijmge-dcopeirzmjm9jcgrscfsykyossao6og60w46duvlx-eml0e_tbxlfzkzw0nqjmquxii5khp-ewuzqvaadaytznzqnsqwt75gnoyu_7vrhqlty6m6ilzjlsuxtycr_wv2fxnf5mm-ebo2glv9laaiqmm_ws3mlcezmzl7kwiqt2met9lnqjb4j-amaw54htuv6skk0_pe1531pgedyzylygrglo4gpliz7htwcclroj2sq0fdzk-uom9w-m2vg-e3_pycuvkhgj2lxlwnwmu4unxpzqtpwyx5jrrwv	https://www.c-info.fi/en/info/?token=u8o8ifesqglzteib.c-zfi2k_0f23pzduelcv3a.48tnvgoaiagujk7z6ijmge-dcopeirzmjm9jcgrscfsykyossao6og60w46duvlx-eml0e_tbxlfzkzw0nqjmquxii5khp-ewuzqvaadaytznzqnsqwt75gnoyu_7vrhqlty6m6ilzjlsuxtycr_wv2fxnf5mm-ebo2glv9laaiqmm_ws3mlcezmzl7kwiqt2met9lnqjb4j-amaw54htuv6skk0_pe1531pgedyzylygrglo4gpliz7htwcclroj2sq0fdzk-uom9w-m2vg-e3_pycuvkhgj2lxlwnwmu4unxpzqtpwyx5jrrwv	PROPN
afs-72416	14	18	regenerate	regenerate	NOUN
afs-72416	14	19	plant	plant	NOUN
afs-72416	14	20	from	from	ADP
afs-72416	14	21	protoplast	protoplast	NOUN
afs-72416	14	22	.	.	PUNCT
afs-72416	15	1	while	while	SCONJ
afs-72416	15	2	the	the	DET
afs-72416	15	3	brassica	brassica	PROPN
afs-72416	15	4	napus	napus	PROPN
afs-72416	15	5	and	and	CCONJ
afs-72416	15	6	the	the	DET
afs-72416	15	7	brassica	brassica	NOUN
afs-72416	15	8	oleracea	oleracea	NOUN
afs-72416	15	9	(	(	PUNCT
afs-72416	15	10	sidney	sidney	PROPN
afs-72416	15	11	et	et	PROPN
afs-72416	15	12	al	al	PROPN
afs-72416	15	13	.	.	PROPN
afs-72416	15	14	1983	1983	NUM
afs-72416	15	15	,	,	PUNCT
afs-72416	15	16	robertson	robertson	PROPN
afs-72416	15	17	et	et	PROPN
afs-72416	15	18	al	al	PROPN
afs-72416	15	19	.	.	PROPN
afs-72416	15	20	1984	1984	NUM
afs-72416	15	21	)	)	PUNCT
afs-72416	15	22	generally	generally	ADV
afs-72416	15	23	give	give	VERB
afs-72416	15	24	good	good	ADJ
afs-72416	15	25	results	result	NOUN
afs-72416	15	26	in	in	ADP
afs-72416	15	27	protoplast	protoplast	NOUN
afs-72416	15	28	regeneration	regeneration	NOUN
afs-72416	15	29	experiments	experiment	NOUN
afs-72416	15	30	(	(	PUNCT
afs-72416	15	31	kartha	kartha	X
afs-72416	15	32	et	et	PROPN
afs-72416	15	33	al	al	PROPN
afs-72416	15	34	.	.	PROPN
afs-72416	15	35	1974	1974	NUM
afs-72416	15	36	,	,	PUNCT
afs-72416	15	37	li	li	PROPN
afs-72416	15	38	et	et	PROPN
afs-72416	15	39	al	al	PROPN
afs-72416	15	40	.	.	PROPN
afs-72416	15	41	1982	1982	NUM
afs-72416	15	42	,	,	PUNCT
afs-72416	15	43	glimelius	glimelius	NOUN
afs-72416	15	44	1984	1984	NUM
afs-72416	15	45	)	)	PUNCT
afs-72416	15	46	,	,	PUNCT
afs-72416	15	47	in	in	ADP
afs-72416	15	48	case	case	NOUN
afs-72416	15	49	of	of	ADP
afs-72416	15	50	turnip	turnip	NOUN
afs-72416	15	51	rape	rape	NOUN
afs-72416	15	52	(	(	PUNCT
afs-72416	15	53	b.	b.	PROPN
afs-72416	15	54	campestris	campestris	PROPN
afs-72416	15	55	,	,	PUNCT
afs-72416	15	56	b.	b.	PROPN
afs-72416	15	57	rapa	rapa	PROPN
afs-72416	15	58	)	)	PUNCT
afs-72416	15	59	the	the	DET
afs-72416	15	60	plant	plant	NOUN
afs-72416	15	61	regeneration	regeneration	NOUN
afs-72416	15	62	from	from	ADP
afs-72416	15	63	protoplast	protoplast	NOUN
afs-72416	15	64	derived	derive	VERB
afs-72416	15	65	calli	calli	NOUN
afs-72416	15	66	remained	remain	VERB
afs-72416	15	67	a	a	DET
afs-72416	15	68	sporadic	sporadic	ADJ
afs-72416	15	69	event	event	NOUN
afs-72416	15	70	(	(	PUNCT
afs-72416	15	71	glimelius	glimelius	NOUN
afs-72416	15	72	1984	1984	NUM
afs-72416	15	73	,	,	PUNCT
afs-72416	15	74	ulrich	ulrich	PROPN
afs-72416	15	75	et	et	PROPN
afs-72416	15	76	al	al	PROPN
afs-72416	15	77	.	.	PROPN
afs-72416	15	78	1980	1980	NUM
afs-72416	15	79	)	)	PUNCT
afs-72416	15	80	.	.	PUNCT
afs-72416	16	1	the	the	DET
afs-72416	16	2	aim	aim	NOUN
afs-72416	16	3	of	of	ADP
afs-72416	16	4	the	the	DET
afs-72416	16	5	present	present	ADJ
afs-72416	16	6	study	study	NOUN
afs-72416	16	7	was	be	AUX
afs-72416	16	8	to	to	PART
afs-72416	16	9	develop	develop	VERB
afs-72416	16	10	a	a	DET
afs-72416	16	11	largely	largely	ADV
afs-72416	16	12	genotype	genotype	NOUN
afs-72416	16	13	independent	independent	ADJ
afs-72416	16	14	,	,	PUNCT
afs-72416	16	15	efficient	efficient	ADJ
afs-72416	16	16	plant	plant	NOUN
afs-72416	16	17	regeneration	regeneration	NOUN
afs-72416	16	18	procedure	procedure	NOUN
afs-72416	16	19	for	for	ADP
afs-72416	16	20	b.	b.	PROPN
afs-72416	16	21	napus	napus	PROPN
afs-72416	16	22	l.	l.	PROPN
afs-72416	16	23	and	and	CCONJ
afs-72416	16	24	b.	b.	PROPN
afs-72416	16	25	campestris	campestris	PROPN
afs-72416	16	26	l.	l.	PROPN
afs-72416	16	27	with	with	ADP
afs-72416	16	28	agronomical	agronomical	ADJ
afs-72416	16	29	importance	importance	NOUN
afs-72416	16	30	.	.	PUNCT
afs-72416	17	1	material	material	NOUN
afs-72416	17	2	and	and	CCONJ
afs-72416	17	3	methods	method	NOUN
afs-72416	17	4	to	to	PART
afs-72416	17	5	extend	extend	VERB
afs-72416	17	6	the	the	DET
afs-72416	17	7	protoplast	protoplast	NOUN
afs-72416	17	8	-	-	PUNCT
afs-72416	17	9	plant	plant	NOUN
afs-72416	17	10	system	system	NOUN
afs-72416	17	11	to	to	ADP
afs-72416	17	12	different	different	ADJ
afs-72416	17	13	genotypes	genotype	NOUN
afs-72416	17	14	of	of	ADP
afs-72416	17	15	agronomic	agronomic	ADJ
afs-72416	17	16	importance	importance	NOUN
afs-72416	17	17	various	various	ADJ
afs-72416	17	18	spring	spring	NOUN
afs-72416	17	19	and	and	CCONJ
afs-72416	17	20	winter	winter	NOUN
afs-72416	17	21	type	type	NOUN
afs-72416	17	22	varieties	variety	NOUN
afs-72416	17	23	were	be	AUX
afs-72416	17	24	included	include	VERB
afs-72416	17	25	in	in	ADP
afs-72416	17	26	the	the	DET
afs-72416	17	27	study	study	NOUN
afs-72416	17	28	.	.	PUNCT
afs-72416	18	1	spring	spring	NOUN
afs-72416	18	2	cultivars	cultivar	NOUN
afs-72416	18	3	of	of	ADP
afs-72416	18	4	brassica	brassica	PROPN
afs-72416	18	5	napus	napus	PROPN
afs-72416	18	6	l.	l.	PROPN
afs-72416	18	7	spp	spp	PROPN
afs-72416	18	8	.	.	PUNCT
afs-72416	19	1	oleifera	oleifera	PROPN
afs-72416	19	2	cv	cv	PROPN
afs-72416	19	3	.	.	PROPN
afs-72416	19	4	hanna	hanna	PROPN
afs-72416	19	5	,	,	PUNCT
afs-72416	19	6	kunto	kunto	PROPN
afs-72416	19	7	,	,	PUNCT
afs-72416	19	8	olga	olga	PROPN
afs-72416	19	9	,	,	PUNCT
afs-72416	19	10	omega	omega	NOUN
afs-72416	19	11	,	,	PUNCT
afs-72416	19	12	varma	varma	PROPN
afs-72416	19	13	,	,	PUNCT
afs-72416	19	14	westar	westar	PROPN
afs-72416	19	15	and	and	CCONJ
afs-72416	19	16	winter	winter	NOUN
afs-72416	19	17	cultivars	cultivar	NOUN
afs-72416	19	18	arabella	arabella	PROPN
afs-72416	19	19	,	,	PUNCT
afs-72416	19	20	belinda	belinda	PROPN
afs-72416	19	21	,	,	PUNCT
afs-72416	19	22	danubia	danubia	NOUN
afs-72416	19	23	,	,	PUNCT
afs-72416	19	24	darmor	darmor	NOUN
afs-72416	19	25	,	,	PUNCT
afs-72416	19	26	santana	santana	PROPN
afs-72416	19	27	,	,	PUNCT
afs-72416	19	28	ujfertodi	ujfertodi	ADV
afs-72416	19	29	,	,	PUNCT
afs-72416	19	30	viking	vike	VERB
afs-72416	19	31	were	be	AUX
afs-72416	19	32	tested	test	VERB
afs-72416	19	33	.	.	PUNCT
afs-72416	20	1	one	one	NUM
afs-72416	20	2	brassica	brassica	PROPN
afs-72416	20	3	campestrisl	campestrisl	PROPN
afs-72416	20	4	.	.	PUNCT
afs-72416	21	1	ssp	ssp	NOUN
afs-72416	21	2	.	.	PUNCT
afs-72416	22	1	oleifera	oleifera	NOUN
afs-72416	22	2	breeding	breed	VERB
afs-72416	22	3	line	line	NOUN
afs-72416	22	4	jo	jo	PROPN
afs-72416	22	5	.	.	PROPN
afs-72416	22	6	4001	4001	NUM
afs-72416	22	7	was	be	AUX
afs-72416	22	8	used	use	VERB
afs-72416	22	9	,	,	PUNCT
afs-72416	22	10	too	too	ADV
afs-72416	22	11	(	(	PUNCT
afs-72416	22	12	table	table	NOUN
afs-72416	22	13	2	2	NUM
afs-72416	22	14	)	)	PUNCT
afs-72416	22	15	.	.	PUNCT
afs-72416	23	1	the	the	DET
afs-72416	23	2	data	datum	NOUN
afs-72416	23	3	were	be	AUX
afs-72416	23	4	calculated	calculate	VERB
afs-72416	23	5	from	from	ADP
afs-72416	23	6	2	2	NUM
afs-72416	23	7	or	or	CCONJ
afs-72416	23	8	3	3	NUM
afs-72416	23	9	independent	independent	ADJ
afs-72416	23	10	experiments	experiment	NOUN
afs-72416	23	11	.	.	PUNCT
afs-72416	24	1	for	for	ADP
afs-72416	24	2	protoplast	protoplast	NOUN
afs-72416	24	3	isolation	isolation	NOUN
afs-72416	24	4	,	,	PUNCT
afs-72416	24	5	the	the	DET
afs-72416	24	6	seeds	seed	NOUN
afs-72416	24	7	were	be	AUX
afs-72416	24	8	surface	surface	NOUN
afs-72416	24	9	sterilized	sterilize	VERB
afs-72416	24	10	with	with	ADP
afs-72416	24	11	75	75	NUM
afs-72416	24	12	%	%	NOUN
afs-72416	24	13	alcohol	alcohol	NOUN
afs-72416	24	14	for	for	ADP
afs-72416	24	15	i—21	i—21	NOUN
afs-72416	24	16	—	—	PUNCT
afs-72416	24	17	2	2	NUM
afs-72416	24	18	minutes	minute	NOUN
afs-72416	24	19	,	,	PUNCT
afs-72416	24	20	then	then	ADV
afs-72416	24	21	immersed	immerse	VERB
afs-72416	24	22	in	in	ADP
afs-72416	24	23	2	2	NUM
afs-72416	24	24	%	%	NOUN
afs-72416	24	25	nao’cl	nao’cl	ADJ
afs-72416	24	26	with	with	ADP
afs-72416	24	27	some	some	DET
afs-72416	24	28	drops	drop	NOUN
afs-72416	24	29	of	of	ADP
afs-72416	24	30	tween	tween	NOUN
afs-72416	24	31	80	80	NUM
afs-72416	24	32	and	and	CCONJ
afs-72416	24	33	continually	continually	ADV
afs-72416	24	34	shaken	shake	VERB
afs-72416	24	35	for	for	ADP
afs-72416	24	36	20	20	NUM
afs-72416	24	37	minutes	minute	NOUN
afs-72416	24	38	followed	follow	VERB
afs-72416	24	39	by	by	ADP
afs-72416	24	40	a	a	DET
afs-72416	24	41	thorough	thorough	ADJ
afs-72416	24	42	wash	wash	NOUN
afs-72416	24	43	in	in	ADP
afs-72416	24	44	autoclaved	autoclaved	ADJ
afs-72416	24	45	sterile	sterile	ADJ
afs-72416	24	46	distilled	distil	VERB
afs-72416	24	47	water	water	NOUN
afs-72416	24	48	.	.	PUNCT
afs-72416	25	1	sterilized	sterilize	VERB
afs-72416	25	2	seeds	seed	NOUN
afs-72416	25	3	were	be	AUX
afs-72416	25	4	germinated	germinate	VERB
afs-72416	25	5	on	on	ADP
afs-72416	25	6	a	a	DET
afs-72416	25	7	hormonfree	hormonfree	ADJ
afs-72416	25	8	ms	ms	NOUN
afs-72416	25	9	medium	medium	ADJ
afs-72416	25	10	(	(	PUNCT
afs-72416	25	11	murashige	murashige	PROPN
afs-72416	25	12	and	and	CCONJ
afs-72416	25	13	skoog	skoog	NOUN
afs-72416	25	14	1962	1962	NUM
afs-72416	25	15	)	)	PUNCT
afs-72416	25	16	for	for	ADP
afs-72416	25	17	5—6	5—6	NUM
afs-72416	25	18	days	day	NOUN
afs-72416	25	19	in	in	ADP
afs-72416	25	20	darkness	darkness	NOUN
afs-72416	25	21	at	at	ADP
afs-72416	25	22	28	28	NUM
afs-72416	25	23	°	°	NOUN
afs-72416	25	24	c	c	NOUN
afs-72416	25	25	.	.	PUNCT
afs-72416	26	1	the	the	DET
afs-72416	26	2	5—6	5—6	NUM
afs-72416	26	3	cm	cm	NOUN
afs-72416	26	4	long	long	ADJ
afs-72416	26	5	hypocotyls	hypocotyls	NOUN
afs-72416	26	6	were	be	AUX
afs-72416	26	7	cut	cut	VERB
afs-72416	26	8	into	into	ADP
afs-72416	26	9	small	small	ADJ
afs-72416	26	10	pieces	piece	NOUN
afs-72416	26	11	of	of	ADP
afs-72416	26	12	about	about	ADV
afs-72416	26	13	1.0	1.0	NUM
afs-72416	26	14	mm	mm	NUM
afs-72416	26	15	and	and	CCONJ
afs-72416	26	16	treated	treat	VERB
afs-72416	26	17	for	for	ADP
afs-72416	26	18	17—18	17—18	NUM
afs-72416	26	19	hours	hour	NOUN
afs-72416	26	20	(	(	PUNCT
afs-72416	26	21	generally	generally	ADV
afs-72416	26	22	overnight	overnight	ADV
afs-72416	26	23	)	)	PUNCT
afs-72416	26	24	with	with	ADP
afs-72416	26	25	1	1	NUM
afs-72416	26	26	%	%	NOUN
afs-72416	26	27	cellulaser-10	cellulaser-10	PROPN
afs-72416	26	28	and	and	CCONJ
afs-72416	26	29	macerozyme	macerozyme	NOUN
afs-72416	26	30	r-10	r-10	DET
afs-72416	26	31	enzymes	enzyme	NOUN
afs-72416	26	32	dissolved	dissolve	VERB
afs-72416	26	33	in	in	ADP
afs-72416	26	34	k	k	PROPN
afs-72416	26	35	3	3	NUM
afs-72416	26	36	(	(	PUNCT
afs-72416	26	37	nagy	nagy	NOUN
afs-72416	26	38	and	and	CCONJ
afs-72416	26	39	maliga	maliga	ADJ
afs-72416	26	40	1976	1976	NUM
afs-72416	26	41	)	)	PUNCT
afs-72416	26	42	medium	medium	NOUN
afs-72416	26	43	.	.	PUNCT
afs-72416	27	1	the	the	DET
afs-72416	27	2	osmotic	osmotic	ADJ
afs-72416	27	3	pressure	pressure	NOUN
afs-72416	27	4	of	of	ADP
afs-72416	27	5	the	the	DET
afs-72416	27	6	enzyme	enzyme	NOUN
afs-72416	27	7	mixture	mixture	NOUN
afs-72416	27	8	was	be	AUX
afs-72416	27	9	raised	raise	VERB
afs-72416	27	10	to	to	ADP
afs-72416	27	11	0.714	0.714	NUM
afs-72416	27	12	m	m	VERB
afs-72416	27	13	using	use	VERB
afs-72416	27	14	mannitol	mannitol	NOUN
afs-72416	27	15	as	as	ADP
afs-72416	27	16	osmotic	osmotic	ADJ
afs-72416	27	17	stabilizer	stabilizer	NOUN
afs-72416	27	18	.	.	PUNCT
afs-72416	28	1	this	this	DET
afs-72416	28	2	treatment	treatment	NOUN
afs-72416	28	3	resulted	result	VERB
afs-72416	28	4	in	in	ADP
afs-72416	28	5	a	a	DET
afs-72416	28	6	great	great	ADJ
afs-72416	28	7	number	number	NOUN
afs-72416	28	8	of	of	ADP
afs-72416	28	9	comparatively	comparatively	ADV
afs-72416	28	10	small	small	ADJ
afs-72416	28	11	protoplasts	protoplast	NOUN
afs-72416	28	12	with	with	ADP
afs-72416	28	13	dense	dense	ADJ
afs-72416	28	14	cytoplasm	cytoplasm	NOUN
afs-72416	28	15	.	.	PUNCT
afs-72416	29	1	the	the	DET
afs-72416	29	2	filtration	filtration	NOUN
afs-72416	29	3	of	of	ADP
afs-72416	29	4	the	the	DET
afs-72416	29	5	isolated	isolate	VERB
afs-72416	29	6	protoplasts	protoplast	NOUN
afs-72416	29	7	was	be	AUX
afs-72416	29	8	omitted	omit	VERB
afs-72416	29	9	to	to	PART
afs-72416	29	10	minimize	minimize	VERB
afs-72416	29	11	physical	physical	ADJ
afs-72416	29	12	damage	damage	NOUN
afs-72416	29	13	.	.	PUNCT
afs-72416	30	1	the	the	DET
afs-72416	30	2	enzyme	enzyme	NOUN
afs-72416	30	3	mixture	mixture	NOUN
afs-72416	30	4	together	together	ADV
afs-72416	30	5	with	with	ADP
afs-72416	30	6	the	the	DET
afs-72416	30	7	protoplast	protoplast	NOUN
afs-72416	30	8	and	and	CCONJ
afs-72416	30	9	the	the	DET
afs-72416	30	10	hypocotyl	hypocotyl	NOUN
afs-72416	30	11	debris	debris	NOUN
afs-72416	30	12	were	be	AUX
afs-72416	30	13	diluted	dilute	VERB
afs-72416	30	14	with	with	ADP
afs-72416	30	15	w	w	PROPN
afs-72416	30	16	5	5	NUM
afs-72416	30	17	salt	salt	NOUN
afs-72416	30	18	solution	solution	NOUN
afs-72416	30	19	(	(	PUNCT
afs-72416	30	20	menczel	menczel	PROPN
afs-72416	30	21	et	et	PROPN
afs-72416	30	22	al	al	PROPN
afs-72416	30	23	.	.	PROPN
afs-72416	30	24	1981	1981	NUM
afs-72416	30	25	)	)	PUNCT
afs-72416	30	26	to	to	ADP
afs-72416	30	27	5—6	5—6	NUM
afs-72416	30	28	times	time	NOUN
afs-72416	30	29	the	the	DET
afs-72416	30	30	original	original	ADJ
afs-72416	30	31	volume	volume	NOUN
afs-72416	30	32	and	and	CCONJ
afs-72416	30	33	centrifuged	centrifuge	VERB
afs-72416	30	34	for	for	ADP
afs-72416	30	35	5	5	NUM
afs-72416	30	36	minutes	minute	NOUN
afs-72416	30	37	at	at	ADP
afs-72416	30	38	120	120	NUM
afs-72416	30	39	g.	g.	NOUN
afs-72416	30	40	the	the	DET
afs-72416	30	41	supernatant	supernatant	NOUN
afs-72416	30	42	was	be	AUX
afs-72416	30	43	discarded	discard	VERB
afs-72416	30	44	and	and	CCONJ
afs-72416	30	45	the	the	DET
afs-72416	30	46	protoplasts	protoplast	NOUN
afs-72416	30	47	,	,	PUNCT
afs-72416	30	48	mixed	mix	VERB
afs-72416	30	49	with	with	ADP
afs-72416	30	50	debris	debris	NOUN
afs-72416	30	51	,	,	PUNCT
afs-72416	30	52	were	be	AUX
afs-72416	30	53	suspended	suspend	VERB
afs-72416	30	54	in	in	ADP
afs-72416	30	55	0,6	0,6	NUM
afs-72416	30	56	m	m	VERB
afs-72416	30	57	sucrose	sucrose	NOUN
afs-72416	30	58	and	and	CCONJ
afs-72416	30	59	w	w	PROPN
afs-72416	30	60	5	5	NUM
afs-72416	30	61	was	be	AUX
afs-72416	30	62	layered	layer	VERB
afs-72416	30	63	on	on	ADP
afs-72416	30	64	the	the	DET
afs-72416	30	65	top	top	NOUN
afs-72416	30	66	,	,	PUNCT
afs-72416	30	67	then	then	ADV
afs-72416	30	68	centrifugated	centrifugate	VERB
afs-72416	30	69	at	at	ADP
afs-72416	30	70	120	120	NUM
afs-72416	30	71	rpm	rpm	NOUN
afs-72416	30	72	for	for	ADP
afs-72416	30	73	5	5	NUM
afs-72416	30	74	minutes	minute	NOUN
afs-72416	30	75	.	.	PUNCT
afs-72416	31	1	the	the	DET
afs-72416	31	2	floating	float	VERB
afs-72416	31	3	protoplasts	protoplast	NOUN
afs-72416	31	4	were	be	AUX
afs-72416	31	5	collected	collect	VERB
afs-72416	31	6	by	by	ADP
afs-72416	31	7	pasteur	pasteur	PROPN
afs-72416	31	8	pipette	pipette	NOUN
afs-72416	31	9	and	and	CCONJ
afs-72416	31	10	washed	wash	VERB
afs-72416	31	11	twice	twice	ADV
afs-72416	31	12	with	with	ADP
afs-72416	31	13	w	w	PROPN
afs-72416	31	14	5	5	NUM
afs-72416	31	15	solution	solution	NOUN
afs-72416	31	16	.	.	PUNCT
afs-72416	32	1	the	the	DET
afs-72416	32	2	isolated	isolated	ADJ
afs-72416	32	3	protoplasts	protoplast	NOUN
afs-72416	32	4	were	be	AUX
afs-72416	32	5	cultured	cultured	ADJ
afs-72416	32	6	in	in	ADP
afs-72416	32	7	0,4	0,4	NUM
afs-72416	32	8	m	m	NOUN
afs-72416	32	9	k-75	k-75	PROPN
afs-72416	32	10	medium	medium	ADJ
afs-72416	32	11	(	(	PUNCT
afs-72416	32	12	kao	kao	NOUN
afs-72416	32	13	and	and	CCONJ
afs-72416	32	14	michayluk	michayluk	PROPN
afs-72416	32	15	1975	1975	NUM
afs-72416	32	16	)	)	PUNCT
afs-72416	32	17	modified	modify	VERB
afs-72416	32	18	by	by	ADP
afs-72416	32	19	glimelius	glimelius	NOUN
afs-72416	32	20	(	(	PUNCT
afs-72416	32	21	1984	1984	NUM
afs-72416	32	22	)	)	PUNCT
afs-72416	32	23	,	,	PUNCT
afs-72416	32	24	at	at	ADP
afs-72416	32	25	28	28	NUM
afs-72416	32	26	°	°	ADP
afs-72416	32	27	c	c	NOUN
afs-72416	32	28	in	in	ADP
afs-72416	32	29	dark	dark	ADJ
afs-72416	32	30	.	.	PUNCT
afs-72416	33	1	protoplasts	protoplast	NOUN
afs-72416	33	2	were	be	AUX
afs-72416	33	3	cultured	cultured	ADJ
afs-72416	33	4	at	at	ADP
afs-72416	33	5	a	a	DET
afs-72416	33	6	density	density	NOUN
afs-72416	33	7	of	of	ADP
afs-72416	33	8	6	6	NUM
afs-72416	33	9	x	x	SYM
afs-72416	33	10	104	104	NUM
afs-72416	33	11	in	in	ADP
afs-72416	33	12	three	three	NUM
afs-72416	33	13	different	different	ADJ
afs-72416	33	14	culture	culture	NOUN
afs-72416	33	15	systems	system	NOUN
afs-72416	33	16	:	:	PUNCT
afs-72416	33	17	(	(	PUNCT
afs-72416	33	18	1	1	X
afs-72416	33	19	)	)	PUNCT
afs-72416	33	20	liquid	liquid	ADJ
afs-72416	33	21	medium	medium	NOUN
afs-72416	33	22	,	,	PUNCT
afs-72416	33	23	(	(	PUNCT
afs-72416	33	24	2	2	X
afs-72416	33	25	)	)	PUNCT
afs-72416	33	26	embedded	embed	VERB
afs-72416	33	27	on	on	ADP
afs-72416	33	28	the	the	DET
afs-72416	33	29	2nd	2nd	ADJ
afs-72416	33	30	day	day	NOUN
afs-72416	33	31	from	from	ADP
afs-72416	33	32	liquid	liquid	NOUN
afs-72416	33	33	into	into	ADP
afs-72416	33	34	agarose	agarose	NOUN
afs-72416	33	35	and	and	CCONJ
afs-72416	33	36	(	(	PUNCT
afs-72416	33	37	3	3	X
afs-72416	33	38	)	)	PUNCT
afs-72416	33	39	embedded	embed	VERB
afs-72416	33	40	into	into	ADP
afs-72416	33	41	agarose	agarose	NOUN
afs-72416	33	42	directly	directly	ADV
afs-72416	33	43	after	after	ADP
afs-72416	33	44	isolation	isolation	NOUN
afs-72416	33	45	.	.	PUNCT
afs-72416	34	1	the	the	DET
afs-72416	34	2	embeddedcultures	embeddedculture	NOUN
afs-72416	34	3	were	be	AUX
afs-72416	34	4	solidified	solidify	VERB
afs-72416	34	5	by	by	ADP
afs-72416	34	6	0,8	0,8	NUM
afs-72416	34	7	%	%	NOUN
afs-72416	34	8	low	low	ADJ
afs-72416	34	9	melting	melting	NOUN
afs-72416	34	10	agarose	agarose	NOUN
afs-72416	34	11	(	(	PUNCT
afs-72416	34	12	sigma	sigma	PROPN
afs-72416	34	13	type	type	NOUN
afs-72416	34	14	vii	vii	PROPN
afs-72416	34	15	)	)	PUNCT
afs-72416	34	16	.	.	PUNCT
afs-72416	35	1	after	after	ADP
afs-72416	35	2	about	about	ADV
afs-72416	35	3	10	10	NUM
afs-72416	35	4	days	day	NOUN
afs-72416	35	5	the	the	DET
afs-72416	35	6	liquid	liquid	NOUN
afs-72416	35	7	and	and	CCONJ
afs-72416	35	8	agarose	agarose	NOUN
afs-72416	35	9	embedded	embed	VERB
afs-72416	35	10	cultures	culture	NOUN
afs-72416	35	11	were	be	AUX
afs-72416	35	12	fed	feed	VERB
afs-72416	35	13	with	with	ADP
afs-72416	35	14	liquid	liquid	ADJ
afs-72416	35	15	medium	medium	NOUN
afs-72416	35	16	with	with	ADP
afs-72416	35	17	reduced	reduced	ADJ
afs-72416	35	18	osmoticum	osmoticum	NOUN
afs-72416	35	19	(	(	PUNCT
afs-72416	35	20	3	3	NUM
afs-72416	35	21	%	%	NOUN
afs-72416	35	22	sucrose	sucrose	NOUN
afs-72416	35	23	)	)	PUNCT
afs-72416	35	24	.	.	PUNCT
afs-72416	36	1	the	the	DET
afs-72416	36	2	cell	cell	NOUN
afs-72416	36	3	aggregates	aggregate	NOUN
afs-72416	36	4	were	be	AUX
afs-72416	36	5	ready	ready	ADJ
afs-72416	36	6	to	to	PART
afs-72416	36	7	plate	plate	VERB
afs-72416	36	8	from	from	ADP
afs-72416	36	9	the	the	DET
afs-72416	36	10	beginning	beginning	NOUN
afs-72416	36	11	of	of	ADP
afs-72416	36	12	the	the	DET
afs-72416	36	13	third	third	ADJ
afs-72416	36	14	week	week	NOUN
afs-72416	36	15	.	.	PUNCT
afs-72416	37	1	the	the	DET
afs-72416	37	2	liquid	liquid	ADJ
afs-72416	37	3	cultures	culture	NOUN
afs-72416	37	4	were	be	AUX
afs-72416	37	5	plated	plate	VERB
afs-72416	37	6	on	on	ADP
afs-72416	37	7	agar	agar	NOUN
afs-72416	37	8	solidified	solidify	VERB
afs-72416	37	9	regeneration	regeneration	NOUN
afs-72416	37	10	medium	medium	NOUN
afs-72416	37	11	and	and	CCONJ
afs-72416	37	12	the	the	DET
afs-72416	37	13	agarose	agarose	NOUN
afs-72416	37	14	blocks	block	NOUN
afs-72416	37	15	were	be	AUX
afs-72416	37	16	floated	float	VERB
afs-72416	37	17	in	in	ADP
afs-72416	37	18	liquid	liquid	ADJ
afs-72416	37	19	medium	medium	NOUN
afs-72416	37	20	before	before	ADP
afs-72416	37	21	plating	plating	NOUN
afs-72416	37	22	.	.	PUNCT
afs-72416	38	1	the	the	DET
afs-72416	38	2	hormones	hormone	NOUN
afs-72416	38	3	were	be	AUX
afs-72416	38	4	decreased	decrease	VERB
afs-72416	38	5	to	to	PART
afs-72416	38	6	quater	quater	NOUN
afs-72416	38	7	strength	strength	NOUN
afs-72416	38	8	in	in	ADP
afs-72416	38	9	the	the	DET
afs-72416	38	10	nutrient	nutrient	NOUN
afs-72416	38	11	and	and	CCONJ
afs-72416	38	12	floating	float	VERB
afs-72416	38	13	medium	medium	NOUN
afs-72416	38	14	as	as	SCONJ
afs-72416	38	15	described	describe	VERB
afs-72416	38	16	by	by	ADP
afs-72416	38	17	vamling	vamle	VERB
afs-72416	38	18	and	and	CCONJ
afs-72416	38	19	glimelius	glimelius	NOUN
afs-72416	38	20	(	(	PUNCT
afs-72416	38	21	1990	1990	NUM
afs-72416	38	22	)	)	PUNCT
afs-72416	38	23	.	.	PUNCT
afs-72416	39	1	the	the	DET
afs-72416	39	2	plant	plant	NOUN
afs-72416	39	3	regeneration	regeneration	NOUN
afs-72416	39	4	was	be	AUX
afs-72416	39	5	carried	carry	VERB
afs-72416	39	6	out	out	ADP
afs-72416	39	7	on	on	ADP
afs-72416	39	8	k	k	PROPN
afs-72416	39	9	3	3	NUM
afs-72416	39	10	medium	medium	NOUN
afs-72416	39	11	supplemented	supplement	VERB
afs-72416	39	12	with	with	ADP
afs-72416	39	13	0.5	0.5	NUM
afs-72416	39	14	mg/1	mg/1	PROPN
afs-72416	39	15	bap	bap	NOUN
afs-72416	39	16	,	,	PUNCT
afs-72416	39	17	0,5	0,5	NUM
afs-72416	39	18	mg/1	mg/1	NOUN
afs-72416	39	19	zeatin	zeatin	NOUN
afs-72416	39	20	and	and	CCONJ
afs-72416	39	21	0,1	0,1	NUM
afs-72416	39	22	mg/1	mg/1	PROPN
afs-72416	39	23	laa	laa	PROPN
afs-72416	39	24	(	(	PUNCT
afs-72416	39	25	nagy	nagy	PROPN
afs-72416	39	26	and	and	CCONJ
afs-72416	39	27	maliga	maliga	ADJ
afs-72416	39	28	1976	1976	NUM
afs-72416	39	29	,	,	PUNCT
afs-72416	39	30	vamling	vamling	NOUN
afs-72416	39	31	and	and	CCONJ
afs-72416	39	32	glimelius	glimelius	NOUN
afs-72416	39	33	1990	1990	NUM
afs-72416	39	34	)	)	PUNCT
afs-72416	39	35	.	.	PUNCT
afs-72416	40	1	to	to	PART
afs-72416	40	2	increase	increase	VERB
afs-72416	40	3	the	the	DET
afs-72416	40	4	efficiency	efficiency	NOUN
afs-72416	40	5	of	of	ADP
afs-72416	40	6	plant	plant	NOUN
afs-72416	40	7	regeneration	regeneration	NOUN
afs-72416	40	8	5	5	NUM
afs-72416	40	9	mg/1	mg/1	NOUN
afs-72416	40	10	agng	agng	ADJ
afs-72416	40	11	3	3	NUM
afs-72416	40	12	was	be	AUX
afs-72416	40	13	added	add	VERB
afs-72416	40	14	to	to	ADP
afs-72416	40	15	the	the	DET
afs-72416	40	16	regeneration	regeneration	NOUN
afs-72416	40	17	medium	medium	NOUN
afs-72416	40	18	.	.	PUNCT
afs-72416	41	1	regenerants	regenerant	NOUN
afs-72416	41	2	were	be	AUX
afs-72416	41	3	grown	grow	VERB
afs-72416	41	4	up	up	ADP
afs-72416	41	5	with	with	ADP
afs-72416	41	6	space	space	NOUN
afs-72416	41	7	isolation	isolation	NOUN
afs-72416	41	8	in	in	ADP
afs-72416	41	9	different	different	ADJ
afs-72416	41	10	greenhouse	greenhouse	NOUN
afs-72416	41	11	chambers	chamber	NOUN
afs-72416	41	12	.	.	PUNCT
afs-72416	42	1	results	result	NOUN
afs-72416	42	2	in	in	ADP
afs-72416	42	3	each	each	DET
afs-72416	42	4	variety	variety	NOUN
afs-72416	42	5	the	the	DET
afs-72416	42	6	protoplasts	protoplast	NOUN
afs-72416	42	7	(	(	PUNCT
afs-72416	42	8	fig	fig	NOUN
afs-72416	42	9	.	.	PUNCT
afs-72416	43	1	la	la	X
afs-72416	43	2	)	)	PUNCT
afs-72416	43	3	372	372	NUM
afs-72416	43	4	were	be	AUX
afs-72416	43	5	isolated	isolate	VERB
afs-72416	43	6	from	from	ADP
afs-72416	43	7	hypocotyl	hypocotyl	PROPN
afs-72416	43	8	.	.	PUNCT
afs-72416	44	1	the	the	DET
afs-72416	44	2	relatively	relatively	ADV
afs-72416	44	3	high	high	ADJ
afs-72416	44	4	osmotic	osmotic	ADJ
afs-72416	44	5	pressure	pressure	NOUN
afs-72416	44	6	(	(	PUNCT
afs-72416	44	7	0,714	0,714	NUM
afs-72416	44	8	m	m	NOUN
afs-72416	44	9	)	)	PUNCT
afs-72416	44	10	results	result	NOUN
afs-72416	44	11	in	in	ADP
afs-72416	44	12	small	small	ADJ
afs-72416	44	13	,	,	PUNCT
afs-72416	44	14	cytoplasm	cytoplasm	ADJ
afs-72416	44	15	-	-	ADJ
afs-72416	44	16	dense	dense	ADJ
afs-72416	44	17	protoplasts	protoplast	NOUN
afs-72416	44	18	.	.	PUNCT
afs-72416	45	1	small	small	ADJ
afs-72416	45	2	,	,	PUNCT
afs-72416	45	3	cytoplasm	cytoplasm	ADJ
afs-72416	45	4	-	-	ADJ
afs-72416	45	5	rich	rich	ADJ
afs-72416	45	6	(	(	PUNCT
afs-72416	45	7	fig	fig	NOUN
afs-72416	45	8	.	.	PUNCT
afs-72416	46	1	lb	lb	X
afs-72416	46	2	)	)	PUNCT
afs-72416	46	3	protoplasts	protoplast	NOUN
afs-72416	46	4	were	be	AUX
afs-72416	46	5	well	well	ADV
afs-72416	46	6	manipulable	manipulable	ADJ
afs-72416	46	7	during	during	ADP
afs-72416	46	8	isolation	isolation	NOUN
afs-72416	46	9	procedure	procedure	NOUN
afs-72416	46	10	and	and	CCONJ
afs-72416	46	11	proved	prove	VERB
afs-72416	46	12	to	to	PART
afs-72416	46	13	be	be	AUX
afs-72416	46	14	excellent	excellent	ADJ
afs-72416	46	15	for	for	ADP
afs-72416	46	16	different	different	ADJ
afs-72416	46	17	culture	culture	NOUN
afs-72416	46	18	procedures	procedure	NOUN
afs-72416	46	19	.	.	PUNCT
afs-72416	47	1	the	the	DET
afs-72416	47	2	culture	culture	NOUN
afs-72416	47	3	methods	method	NOUN
afs-72416	47	4	applied	apply	VERB
afs-72416	47	5	resulted	result	VERB
afs-72416	47	6	in	in	ADP
afs-72416	47	7	difference	difference	NOUN
afs-72416	47	8	in	in	ADP
afs-72416	47	9	initiation	initiation	NOUN
afs-72416	47	10	of	of	ADP
afs-72416	47	11	cell	cell	NOUN
afs-72416	47	12	division	division	NOUN
afs-72416	47	13	at	at	ADP
afs-72416	47	14	24hour	24hour	NUM
afs-72416	47	15	age	age	NOUN
afs-72416	47	16	(	(	PUNCT
afs-72416	47	17	table	table	NOUN
afs-72416	47	18	1	1	NUM
afs-72416	47	19	)	)	PUNCT
afs-72416	47	20	.	.	PUNCT
afs-72416	48	1	protoplasts	protoplast	NOUN
afs-72416	48	2	cultured	cultured	ADJ
afs-72416	48	3	in	in	ADP
afs-72416	48	4	liqfig	liqfig	PROPN
afs-72416	48	5	.	.	PUNCT
afs-72416	49	1	i.	i.	PROPN
afs-72416	49	2	development	development	PROPN
afs-72416	49	3	of	of	ADP
afs-72416	49	4	brassica	brassica	NOUN
afs-72416	49	5	protoplast	protoplast	NOUN
afs-72416	49	6	-	-	PUNCT
afs-72416	49	7	callus	callus	ADJ
afs-72416	49	8	system	system	NOUN
afs-72416	49	9	using	use	VERB
afs-72416	49	10	agarose	agarose	NOUN
afs-72416	49	11	embedding	embed	VERB
afs-72416	49	12	technique	technique	NOUN
afs-72416	49	13	with	with	ADP
afs-72416	49	14	’	'	PUNCT
afs-72416	49	15	varma	varma	PROPN
afs-72416	49	16	’	'	PUNCT
afs-72416	49	17	cv	cv	PROPN
afs-72416	49	18	.	.	PUNCT
afs-72416	49	19	a.	a.	NOUN
afs-72416	49	20	freshly	freshly	ADV
afs-72416	49	21	-	-	PUNCT
afs-72416	49	22	isolated	isolate	VERB
afs-72416	49	23	protoplasts	protoplast	NOUN
afs-72416	49	24	collected	collect	VERB
afs-72416	49	25	from	from	ADP
afs-72416	49	26	the	the	DET
afs-72416	49	27	interphase	interphase	NOUN
afs-72416	49	28	ofgradient	ofgradient	NOUN
afs-72416	49	29	centrifugation	centrifugation	NOUN
afs-72416	49	30	.	.	PUNCT
afs-72416	50	1	bar	bar	NOUN
afs-72416	50	2	=	=	SYM
afs-72416	50	3	40	40	NUM
afs-72416	50	4	pm	pm	NOUN
afs-72416	50	5	.	.	PUNCT
afs-72416	51	1	b.	b.	PROPN
afs-72416	51	2	cytoplasmrich	cytoplasmrich	PROPN
afs-72416	51	3	protoplast	protoplast	NOUN
afs-72416	51	4	embedded	embed	VERB
afs-72416	51	5	in	in	ADP
afs-72416	51	6	agarose	agarose	NOUN
afs-72416	51	7	solidified	solidify	VERB
afs-72416	51	8	medium	medium	ADJ
afs-72416	51	9	.	.	PUNCT
afs-72416	52	1	bar	bar	NOUN
afs-72416	52	2	=	=	SYM
afs-72416	52	3	40	40	NUM
afs-72416	52	4	pm	pm	NOUN
afs-72416	52	5	.	.	PUNCT
afs-72416	53	1	c.	c.	NOUN
afs-72416	53	2	freshly	freshly	ADV
afs-72416	53	3	divided	divide	VERB
afs-72416	53	4	protoplast	protoplast	NOUN
afs-72416	53	5	embedded	embed	VERB
afs-72416	53	6	into	into	ADP
afs-72416	53	7	agarose	agarose	NOUN
afs-72416	53	8	from	from	ADP
afs-72416	53	9	liquid	liquid	ADJ
afs-72416	53	10	medium	medium	NOUN
afs-72416	53	11	on	on	ADP
afs-72416	53	12	the	the	DET
afs-72416	53	13	second	second	ADJ
afs-72416	53	14	day	day	NOUN
afs-72416	53	15	of	of	ADP
afs-72416	53	16	culturing	culturing	NOUN
afs-72416	53	17	.	.	PUNCT
afs-72416	54	1	bar	bar	NOUN
afs-72416	54	2	=	=	SYM
afs-72416	54	3	40	40	NUM
afs-72416	54	4	pm	pm	NOUN
afs-72416	54	5	.	.	PUNCT
afs-72416	55	1	d.	d.	PROPN
afs-72416	55	2	second	second	ADJ
afs-72416	55	3	cell	cell	NOUN
afs-72416	55	4	division	division	NOUN
afs-72416	55	5	within	within	ADP
afs-72416	55	6	4	4	NUM
afs-72416	55	7	days	day	NOUN
afs-72416	55	8	of	of	ADP
afs-72416	55	9	culturing	culturing	NOUN
afs-72416	55	10	.	.	PUNCT
afs-72416	56	1	bar	bar	NOUN
afs-72416	56	2	=	=	SYM
afs-72416	56	3	40	40	NUM
afs-72416	56	4	pm	pm	NOUN
afs-72416	56	5	.	.	PUNCT
afs-72416	57	1	e.	e.	PROPN
afs-72416	57	2	cell	cell	PROPN
afs-72416	57	3	clusters	cluster	NOUN
afs-72416	57	4	obtained	obtain	VERB
afs-72416	57	5	within	within	ADP
afs-72416	57	6	40	40	NUM
afs-72416	57	7	days	day	NOUN
afs-72416	57	8	of	of	ADP
afs-72416	57	9	culturing	culturing	NOUN
afs-72416	57	10	.	.	PUNCT
afs-72416	58	1	bar	bar	NOUN
afs-72416	58	2	=	=	SYM
afs-72416	58	3	0,1	0,1	NUM
afs-72416	58	4	mm	mm	NOUN
afs-72416	58	5	.	.	PUNCT
afs-72416	59	1	f.	f.	PROPN
afs-72416	59	2	protoplast	protoplast	NOUN
afs-72416	59	3	-	-	PUNCT
afs-72416	59	4	derived	derive	VERB
afs-72416	59	5	colonies	colony	NOUN
afs-72416	59	6	of	of	ADP
afs-72416	59	7	’	'	PUNCT
afs-72416	59	8	varma	varma	PROPN
afs-72416	59	9	’	'	PUNCT
afs-72416	59	10	cultivar	cultivar	NOUN
afs-72416	59	11	in	in	ADP
afs-72416	59	12	agarose	agarose	NOUN
afs-72416	59	13	-	-	PUNCT
afs-72416	59	14	solidified	solidify	VERB
afs-72416	59	15	medium	medium	NOUN
afs-72416	59	16	and	and	CCONJ
afs-72416	59	17	agarose	agarose	NOUN
afs-72416	59	18	blocks	block	NOUN
afs-72416	59	19	floated	float	VERB
afs-72416	59	20	in	in	ADP
afs-72416	59	21	liquid	liquid	ADJ
afs-72416	59	22	medium	medium	NOUN
afs-72416	59	23	.	.	PUNCT
afs-72416	60	1	magnification	magnification	NOUN
afs-72416	60	2	=	=	SYM
afs-72416	60	3	2x	2x	NUM
afs-72416	60	4	.	.	PUNCT
afs-72416	61	1	373	373	NUM
afs-72416	61	2	table	table	NOUN
afs-72416	61	3	i.	i.	NOUN
afs-72416	61	4	percentage	percentage	NOUN
afs-72416	61	5	of	of	ADP
afs-72416	61	6	dividingcells	dividingcell	NOUN
afs-72416	61	7	using	use	VERB
afs-72416	61	8	three	three	NUM
afs-72416	61	9	different	different	ADJ
afs-72416	61	10	culture	culture	NOUN
afs-72416	61	11	methods	method	NOUN
afs-72416	61	12	in	in	ADP
afs-72416	61	13	protoplast	protoplast	NOUN
afs-72416	61	14	cultures	culture	NOUN
afs-72416	61	15	of	of	ADP
afs-72416	61	16	three	three	NUM
afs-72416	61	17	b.	b.	PROPN
afs-72416	61	18	napus	napus	PROPN
afs-72416	61	19	cv	cv	PROPN
afs-72416	61	20	.	.	PUNCT
afs-72416	62	1	the	the	DET
afs-72416	62	2	numbers	number	NOUN
afs-72416	62	3	of	of	ADP
afs-72416	62	4	division	division	NOUN
afs-72416	62	5	(	(	PUNCT
afs-72416	62	6	in	in	ADP
afs-72416	62	7	percent	percent	NOUN
afs-72416	62	8	)	)	PUNCT
afs-72416	62	9	were	be	AUX
afs-72416	62	10	counted	count	VERB
afs-72416	62	11	after	after	ADP
afs-72416	62	12	24-	24-	NUM
afs-72416	62	13	,	,	PUNCT
afs-72416	62	14	48and	48and	NOUN
afs-72416	62	15	96	96	NUM
afs-72416	62	16	hours	hour	NOUN
afs-72416	62	17	.	.	PUNCT
afs-72416	63	1	genotype	genotype	NOUN
afs-72416	63	2	culture	culture	NOUN
afs-72416	63	3	percentage	percentage	NOUN
afs-72416	63	4	of	of	ADP
afs-72416	63	5	dividing	divide	VERB
afs-72416	63	6	method	method	NOUN
afs-72416	63	7	*	*	PUNCT
afs-72416	63	8	cells	cell	NOUN
afs-72416	63	9	after	after	SCONJ
afs-72416	63	10	24	24	NUM
afs-72416	63	11	48	48	NUM
afs-72416	63	12	96	96	NUM
afs-72416	63	13	hours	hour	NOUN
afs-72416	63	14	hours	hour	NOUN
afs-72416	63	15	hours	hour	NOUN
afs-72416	63	16	liquid	liquid	ADJ
afs-72416	63	17	1,1	1,1	NUM
afs-72416	63	18	18	18	NUM
afs-72416	63	19	48	48	NUM
afs-72416	63	20	emb	emb	NOUN
afs-72416	63	21	.	.	PUNCT
afs-72416	64	1	2nd	2nd	PROPN
afs-72416	64	2	d.	d.	PROPN
afs-72416	64	3	1,0	1,0	NUM
afs-72416	64	4	14	14	NUM
afs-72416	64	5	46	46	NUM
afs-72416	64	6	emb	emb	NOUN
afs-72416	64	7	.	.	PUNCT
afs-72416	65	1	direct	direct	ADJ
afs-72416	65	2	0	0	NUM
afs-72416	65	3	16	16	NUM
afs-72416	65	4	47	47	NUM
afs-72416	65	5	olga	olga	NOUN
afs-72416	65	6	liquid	liquid	PROPN
afs-72416	65	7	2,3	2,3	NUM
afs-72416	65	8	12	12	NUM
afs-72416	65	9	51	51	NUM
afs-72416	65	10	varma	varma	PROPN
afs-72416	65	11	emb	emb	PROPN
afs-72416	65	12	.	.	PUNCT
afs-72416	66	1	2nd	2nd	PROPN
afs-72416	66	2	d.	d.	PROPN
afs-72416	66	3	2,4	2,4	NUM
afs-72416	66	4	14	14	NUM
afs-72416	66	5	53	53	NUM
afs-72416	66	6	emb	emb	NOUN
afs-72416	66	7	.	.	PUNCT
afs-72416	67	1	direct	direct	ADJ
afs-72416	67	2	0,1	0,1	NUM
afs-72416	67	3	10	10	NUM
afs-72416	67	4	49	49	NUM
afs-72416	67	5	liquid	liquid	ADJ
afs-72416	67	6	0,7	0,7	NUM
afs-72416	67	7	9	9	NUM
afs-72416	67	8	44	44	NUM
afs-72416	67	9	arabella	arabella	PROPN
afs-72416	67	10	emb	emb	PROPN
afs-72416	67	11	.	.	PUNCT
afs-72416	68	1	2nd	2nd	PROPN
afs-72416	68	2	d.	d.	PROPN
afs-72416	68	3	0,9	0,9	NUM
afs-72416	68	4	7	7	NUM
afs-72416	68	5	42	42	NUM
afs-72416	68	6	emb	emb	NOUN
afs-72416	68	7	.	.	PUNCT
afs-72416	69	1	direct	direct	ADJ
afs-72416	69	2	0	0	NUM
afs-72416	69	3	7	7	NUM
afs-72416	69	4	45	45	NUM
afs-72416	69	5	*	*	PUNCT
afs-72416	69	6	see	see	VERB
afs-72416	69	7	in	in	ADP
afs-72416	69	8	materials	material	NOUN
afs-72416	69	9	and	and	CCONJ
afs-72416	69	10	methods	method	NOUN
afs-72416	69	11	uid	uid	PROPN
afs-72416	69	12	medium	medium	NOUN
afs-72416	69	13	were	be	AUX
afs-72416	69	14	able	able	ADJ
afs-72416	69	15	to	to	PART
afs-72416	69	16	undergo	undergo	VERB
afs-72416	69	17	divisions	division	NOUN
afs-72416	69	18	from	from	ADP
afs-72416	69	19	the	the	DET
afs-72416	69	20	end	end	NOUN
afs-72416	69	21	of	of	ADP
afs-72416	69	22	the	the	DET
afs-72416	69	23	ist	ist	NOUN
afs-72416	69	24	day	day	NOUN
afs-72416	69	25	depending	depend	VERB
afs-72416	69	26	on	on	ADP
afs-72416	69	27	culture	culture	NOUN
afs-72416	69	28	methods	method	NOUN
afs-72416	69	29	.	.	PUNCT
afs-72416	70	1	protoplasts	protoplast	NOUN
afs-72416	70	2	embedded	embed	VERB
afs-72416	70	3	directly	directly	ADV
afs-72416	70	4	after	after	ADP
afs-72416	70	5	isolation	isolation	NOUN
afs-72416	70	6	into	into	ADP
afs-72416	70	7	agarose	agarose	NOUN
afs-72416	70	8	were	be	AUX
afs-72416	70	9	capable	capable	ADJ
afs-72416	70	10	to	to	PART
afs-72416	70	11	divide	divide	VERB
afs-72416	70	12	from	from	ADP
afs-72416	70	13	the	the	DET
afs-72416	70	14	2nd	2nd	ADJ
afs-72416	70	15	day	day	NOUN
afs-72416	70	16	of	of	ADP
afs-72416	70	17	culturing	culture	VERB
afs-72416	70	18	showing	show	VERB
afs-72416	70	19	visible	visible	ADJ
afs-72416	70	20	divisions	division	NOUN
afs-72416	70	21	at	at	ADP
afs-72416	70	22	48th	48th	ADJ
afs-72416	70	23	hour	hour	NOUN
afs-72416	70	24	(	(	PUNCT
afs-72416	70	25	fig	fig	NOUN
afs-72416	70	26	.	.	PUNCT
afs-72416	71	1	1c	1c	NOUN
afs-72416	71	2	)	)	PUNCT
afs-72416	71	3	.	.	PUNCT
afs-72416	72	1	the	the	DET
afs-72416	72	2	agarose	agarose	NOUN
afs-72416	72	3	technique	technique	NOUN
afs-72416	72	4	seems	seem	VERB
afs-72416	72	5	to	to	PART
afs-72416	72	6	be	be	AUX
afs-72416	72	7	a	a	DET
afs-72416	72	8	hindering	hinder	VERB
afs-72416	72	9	factor	factor	NOUN
afs-72416	72	10	in	in	ADP
afs-72416	72	11	the	the	DET
afs-72416	72	12	starting	starting	NOUN
afs-72416	72	13	of	of	ADP
afs-72416	72	14	cell	cell	NOUN
afs-72416	72	15	division	division	NOUN
afs-72416	72	16	,	,	PUNCT
afs-72416	72	17	but	but	CCONJ
afs-72416	72	18	this	this	DET
afs-72416	72	19	hindering	hinder	VERB
afs-72416	72	20	effect	effect	NOUN
afs-72416	72	21	disappeared	disappear	VERB
afs-72416	72	22	on	on	ADP
afs-72416	72	23	the	the	DET
afs-72416	72	24	fourth	fourth	ADJ
afs-72416	72	25	day	day	NOUN
afs-72416	72	26	of	of	ADP
afs-72416	72	27	culturing	culturing	NOUN
afs-72416	72	28	.	.	PUNCT
afs-72416	73	1	there	there	PRON
afs-72416	73	2	was	be	VERB
afs-72416	73	3	no	no	DET
afs-72416	73	4	significant	significant	ADJ
afs-72416	73	5	difference	difference	NOUN
afs-72416	73	6	in	in	ADP
afs-72416	73	7	percentage	percentage	NOUN
afs-72416	73	8	of	of	ADP
afs-72416	73	9	dividing	divide	VERB
afs-72416	73	10	cells	cell	NOUN
afs-72416	73	11	obtained	obtain	VERB
afs-72416	73	12	from	from	ADP
afs-72416	73	13	different	different	ADJ
afs-72416	73	14	varieties	variety	NOUN
afs-72416	73	15	using	use	VERB
afs-72416	73	16	different	different	ADJ
afs-72416	73	17	culture	culture	NOUN
afs-72416	73	18	techniques	technique	NOUN
afs-72416	73	19	on	on	ADP
afs-72416	73	20	the	the	DET
afs-72416	73	21	fourth	fourth	ADJ
afs-72416	73	22	day	day	NOUN
afs-72416	73	23	of	of	ADP
afs-72416	73	24	culturing	culture	VERB
afs-72416	73	25	(	(	PUNCT
afs-72416	73	26	table	table	NOUN
afs-72416	73	27	1	1	NUM
afs-72416	73	28	)	)	PUNCT
afs-72416	73	29	.	.	PUNCT
afs-72416	74	1	each	each	DET
afs-72416	74	2	variety	variety	NOUN
afs-72416	74	3	produced	produce	VERB
afs-72416	74	4	about	about	ADP
afs-72416	74	5	the	the	DET
afs-72416	74	6	same	same	ADJ
afs-72416	74	7	percentage	percentage	NOUN
afs-72416	74	8	of	of	ADP
afs-72416	74	9	divisions	division	NOUN
afs-72416	74	10	on	on	ADP
afs-72416	74	11	the	the	DET
afs-72416	74	12	fourth	fourth	ADJ
afs-72416	74	13	day	day	NOUN
afs-72416	74	14	(	(	PUNCT
afs-72416	74	15	in	in	ADP
afs-72416	74	16	96	96	NUM
afs-72416	74	17	hours	hour	NOUN
afs-72416	74	18	)	)	PUNCT
afs-72416	74	19	of	of	ADP
afs-72416	74	20	subculturing	subculturing	NOUN
afs-72416	74	21	.	.	PUNCT
afs-72416	75	1	second	second	ADJ
afs-72416	75	2	divisions	division	NOUN
afs-72416	75	3	(	(	PUNCT
afs-72416	75	4	fig	fig	NOUN
afs-72416	75	5	.	.	PUNCT
afs-72416	76	1	i	i	PRON
afs-72416	76	2	d	d	PROPN
afs-72416	76	3	)	)	PUNCT
afs-72416	76	4	were	be	AUX
afs-72416	76	5	observed	observe	VERB
afs-72416	76	6	from	from	ADP
afs-72416	76	7	the	the	DET
afs-72416	76	8	third	third	ADJ
afs-72416	76	9	day	day	NOUN
afs-72416	76	10	of	of	ADP
afs-72416	76	11	subculturing	subculturing	NOUN
afs-72416	76	12	.	.	PUNCT
afs-72416	77	1	among	among	ADP
afs-72416	77	2	the	the	DET
afs-72416	77	3	varieties	variety	NOUN
afs-72416	77	4	,	,	PUNCT
afs-72416	77	5	varma	varma	PROPN
afs-72416	77	6	was	be	AUX
afs-72416	77	7	the	the	DET
afs-72416	77	8	most	most	ADV
afs-72416	77	9	responsive	responsive	ADJ
afs-72416	77	10	in	in	ADP
afs-72416	77	11	the	the	DET
afs-72416	77	12	efficiency	efficiency	NOUN
afs-72416	77	13	of	of	ADP
afs-72416	77	14	cell	cell	NOUN
afs-72416	77	15	division	division	NOUN
afs-72416	77	16	at	at	ADP
afs-72416	77	17	the	the	DET
afs-72416	77	18	end	end	NOUN
afs-72416	77	19	of	of	ADP
afs-72416	77	20	the	the	DET
afs-72416	77	21	4th	4th	ADJ
afs-72416	77	22	day	day	NOUN
afs-72416	77	23	of	of	ADP
afs-72416	77	24	culture	culture	NOUN
afs-72416	77	25	,	,	PUNCT
afs-72416	77	26	but	but	CCONJ
afs-72416	77	27	in	in	ADP
afs-72416	77	28	commencement	commencement	NOUN
afs-72416	77	29	of	of	ADP
afs-72416	77	30	microcolony	microcolony	PROPN
afs-72416	77	31	development	development	PROPN
afs-72416	77	32	there	there	PRON
afs-72416	77	33	was	be	VERB
afs-72416	77	34	no	no	DET
afs-72416	77	35	substantial	substantial	ADJ
afs-72416	77	36	difference	difference	NOUN
afs-72416	77	37	between	between	ADP
afs-72416	77	38	culture	culture	NOUN
afs-72416	77	39	methods	method	NOUN
afs-72416	77	40	regarding	regard	VERB
afs-72416	77	41	the	the	DET
afs-72416	77	42	percentage	percentage	NOUN
afs-72416	77	43	of	of	ADP
afs-72416	77	44	developed	develop	VERB
afs-72416	77	45	colonies	colony	NOUN
afs-72416	77	46	.	.	PUNCT
afs-72416	78	1	observing	observe	VERB
afs-72416	78	2	of	of	ADP
afs-72416	78	3	cell	cell	NOUN
afs-72416	78	4	division	division	NOUN
afs-72416	78	5	after	after	ADP
afs-72416	78	6	96	96	NUM
afs-72416	78	7	hours	hour	NOUN
afs-72416	78	8	of	of	ADP
afs-72416	78	9	culturing	culturing	NOUN
afs-72416	78	10	was	be	AUX
afs-72416	78	11	easier	easy	ADJ
afs-72416	78	12	in	in	ADP
afs-72416	78	13	agarose	agarose	NOUN
afs-72416	78	14	embedded	embed	VERB
afs-72416	78	15	cultures	culture	NOUN
afs-72416	78	16	because	because	SCONJ
afs-72416	78	17	the	the	DET
afs-72416	78	18	well	well	ADV
afs-72416	78	19	-	-	PUNCT
afs-72416	78	20	spread	spread	VERB
afs-72416	78	21	cells	cell	NOUN
afs-72416	78	22	,	,	PUNCT
afs-72416	78	23	protoplastderived	protoplastderive	VERB
afs-72416	78	24	colonies	colony	NOUN
afs-72416	78	25	in	in	ADP
afs-72416	78	26	agarose	agarose	PROPN
afs-72416	78	27	were	be	AUX
afs-72416	78	28	in	in	ADP
afs-72416	78	29	fixed	fix	VERB
afs-72416	78	30	positions	position	NOUN
afs-72416	78	31	while	while	SCONJ
afs-72416	78	32	in	in	ADP
afs-72416	78	33	liquid	liquid	ADJ
afs-72416	78	34	medium	medium	NOUN
afs-72416	78	35	the	the	DET
afs-72416	78	36	cells	cell	NOUN
afs-72416	78	37	were	be	AUX
afs-72416	78	38	aggregated	aggregate	VERB
afs-72416	78	39	spontaneously	spontaneously	ADV
afs-72416	78	40	and	and	CCONJ
afs-72416	78	41	it	it	PRON
afs-72416	78	42	was	be	AUX
afs-72416	78	43	difficult	difficult	ADJ
afs-72416	78	44	to	to	PART
afs-72416	78	45	distinguish	distinguish	VERB
afs-72416	78	46	between	between	ADP
afs-72416	78	47	division	division	NOUN
afs-72416	78	48	-	-	PUNCT
afs-72416	78	49	derived	derive	VERB
afs-72416	78	50	microcolonies	microcolonie	NOUN
afs-72416	78	51	and	and	CCONJ
afs-72416	78	52	spontaneous	spontaneous	ADJ
afs-72416	78	53	cell	cell	NOUN
afs-72416	78	54	groups	group	NOUN
afs-72416	78	55	.	.	PUNCT
afs-72416	79	1	after	after	SCONJ
afs-72416	79	2	about	about	ADV
afs-72416	79	3	ten	ten	NUM
afs-72416	79	4	days	day	NOUN
afs-72416	79	5	in	in	ADP
afs-72416	79	6	cultures	culture	NOUN
afs-72416	79	7	small	small	ADJ
afs-72416	79	8	calli	calli	NOUN
afs-72416	79	9	or	or	CCONJ
afs-72416	79	10	micro	micro	ADJ
afs-72416	79	11	colonies	colony	NOUN
afs-72416	79	12	(	(	PUNCT
afs-72416	79	13	fig	fig	NOUN
afs-72416	79	14	.	.	PUNCT
afs-72416	79	15	le	le	X
afs-72416	79	16	)	)	PUNCT
afs-72416	79	17	had	have	AUX
afs-72416	79	18	developed	develop	VERB
afs-72416	79	19	in	in	ADP
afs-72416	79	20	both	both	DET
afs-72416	79	21	culture	culture	NOUN
afs-72416	79	22	systems	system	NOUN
afs-72416	79	23	.	.	PUNCT
afs-72416	80	1	there	there	PRON
afs-72416	80	2	was	be	VERB
afs-72416	80	3	an	an	DET
afs-72416	80	4	essential	essential	ADJ
afs-72416	80	5	difference	difference	NOUN
afs-72416	80	6	between	between	ADP
afs-72416	80	7	liquid	liquid	ADJ
afs-72416	80	8	culture	culture	NOUN
afs-72416	80	9	technique	technique	NOUN
afs-72416	80	10	and	and	CCONJ
afs-72416	80	11	agarose	agarose	NOUN
afs-72416	80	12	solidified	solidify	VERB
afs-72416	80	13	culture	culture	NOUN
afs-72416	80	14	.	.	PUNCT
afs-72416	81	1	in	in	ADP
afs-72416	81	2	the	the	DET
afs-72416	81	3	liquid	liquid	ADJ
afs-72416	81	4	cultures	culture	NOUN
afs-72416	81	5	a	a	DET
afs-72416	81	6	brown	brown	ADJ
afs-72416	81	7	precipitation	precipitation	NOUN
afs-72416	81	8	started	start	VERB
afs-72416	81	9	to	to	PART
afs-72416	81	10	develop	develop	VERB
afs-72416	81	11	around	around	ADP
afs-72416	81	12	microcalli	microcalli	NOUN
afs-72416	81	13	from	from	ADP
afs-72416	81	14	the	the	DET
afs-72416	81	15	second	second	ADJ
afs-72416	81	16	week	week	NOUN
afs-72416	81	17	.	.	PUNCT
afs-72416	82	1	this	this	DET
afs-72416	82	2	phenomenon	phenomenon	NOUN
afs-72416	82	3	,	,	PUNCT
afs-72416	82	4	observed	observe	VERB
afs-72416	82	5	by	by	ADP
afs-72416	82	6	other	other	ADJ
afs-72416	82	7	authors	author	NOUN
afs-72416	82	8	as	as	ADV
afs-72416	82	9	well	well	ADV
afs-72416	82	10	(	(	PUNCT
afs-72416	82	11	schenck	schenck	NOUN
afs-72416	82	12	and	and	CCONJ
afs-72416	82	13	hoffman	hoffman	NOUN
afs-72416	82	14	1979	1979	NUM
afs-72416	82	15	,	,	PUNCT
afs-72416	82	16	glimelius	glimelius	NOUN
afs-72416	82	17	1984	1984	NUM
afs-72416	82	18	)	)	PUNCT
afs-72416	82	19	,	,	PUNCT
afs-72416	82	20	was	be	AUX
afs-72416	82	21	detrimental	detrimental	ADJ
afs-72416	82	22	to	to	ADP
afs-72416	82	23	the	the	DET
afs-72416	82	24	subsequent	subsequent	ADJ
afs-72416	82	25	culture	culture	NOUN
afs-72416	82	26	work	work	NOUN
afs-72416	82	27	,	,	PUNCT
afs-72416	82	28	inhibiting	inhibit	VERB
afs-72416	82	29	or	or	CCONJ
afs-72416	82	30	completely	completely	ADV
afs-72416	82	31	stopping	stop	VERB
afs-72416	82	32	the	the	DET
afs-72416	82	33	growth	growth	NOUN
afs-72416	82	34	.	.	PUNCT
afs-72416	83	1	this	this	DET
afs-72416	83	2	harmful	harmful	ADJ
afs-72416	83	3	phenomenon	phenomenon	NOUN
afs-72416	83	4	was	be	AUX
afs-72416	83	5	not	not	PART
afs-72416	83	6	observed	observe	VERB
afs-72416	83	7	in	in	ADP
afs-72416	83	8	agarose	agarose	NOUN
afs-72416	83	9	solidifiedcultures	solidifiedculture	NOUN
afs-72416	83	10	(	(	PUNCT
afs-72416	83	11	fig	fig	NOUN
afs-72416	83	12	.	.	PUNCT
afs-72416	84	1	if	if	SCONJ
afs-72416	84	2	)	)	PUNCT
afs-72416	84	3	indicating	indicate	VERB
afs-72416	84	4	more	more	ADV
afs-72416	84	5	favourable	favourable	ADJ
afs-72416	84	6	outcome	outcome	NOUN
afs-72416	84	7	when	when	SCONJ
afs-72416	84	8	using	use	VERB
afs-72416	84	9	agarose	agarose	NOUN
afs-72416	84	10	-	-	PUNCT
afs-72416	84	11	embedding	embedding	NOUN
afs-72416	84	12	in	in	ADP
afs-72416	84	13	brassica	brassica	NOUN
afs-72416	84	14	protoplast	protoplast	NOUN
afs-72416	84	15	culture	culture	NOUN
afs-72416	84	16	.	.	PUNCT
afs-72416	85	1	except	except	SCONJ
afs-72416	85	2	for	for	ADP
afs-72416	85	3	one	one	NUM
afs-72416	85	4	variety	variety	NOUN
afs-72416	85	5	ujfertddi	ujfertddi	NOUN
afs-72416	85	6	—	—	PUNCT
afs-72416	85	7	,	,	PUNCT
afs-72416	85	8	every	every	DET
afs-72416	85	9	variety	variety	NOUN
afs-72416	85	10	in	in	ADP
afs-72416	85	11	both	both	CCONJ
afs-72416	85	12	brassica	brassica	NOUN
afs-72416	85	13	napus	napus	PROPN
afs-72416	85	14	and	and	CCONJ
afs-72416	85	15	campestris	campestris	PROPN
afs-72416	85	16	formed	form	VERB
afs-72416	85	17	a	a	DET
afs-72416	85	18	higher	high	ADJ
afs-72416	85	19	number	number	NOUN
afs-72416	85	20	of	of	ADP
afs-72416	85	21	calli	calli	NOUN
afs-72416	85	22	for	for	ADP
afs-72416	85	23	plant	plant	NOUN
afs-72416	85	24	regeneration	regeneration	NOUN
afs-72416	85	25	experiments	experiment	NOUN
afs-72416	85	26	(	(	PUNCT
afs-72416	85	27	table	table	NOUN
afs-72416	85	28	2	2	NUM
afs-72416	85	29	)	)	PUNCT
afs-72416	85	30	.	.	PUNCT
afs-72416	86	1	at	at	ADP
afs-72416	86	2	the	the	DET
afs-72416	86	3	end	end	NOUN
afs-72416	86	4	of	of	ADP
afs-72416	86	5	2	2	NUM
afs-72416	86	6	nd	nd	ADV
afs-72416	86	7	and	and	CCONJ
afs-72416	86	8	3rd	3rd	ADJ
afs-72416	86	9	week	week	NOUN
afs-72416	86	10	,	,	PUNCT
afs-72416	86	11	the	the	DET
afs-72416	86	12	number	number	NOUN
afs-72416	86	13	of	of	ADP
afs-72416	86	14	formed	form	VERB
afs-72416	86	15	microcalli	microcalli	NOUN
afs-72416	86	16	varied	varied	ADJ
afs-72416	86	17	,	,	PUNCT
afs-72416	86	18	depending	depend	VERB
afs-72416	86	19	on	on	ADP
afs-72416	86	20	individual	individual	ADJ
afs-72416	86	21	cultures	culture	NOUN
afs-72416	86	22	and	and	CCONJ
afs-72416	86	23	genotypes	genotype	NOUN
afs-72416	86	24	,	,	PUNCT
afs-72416	86	25	but	but	CCONJ
afs-72416	86	26	no	no	DET
afs-72416	86	27	essential	essential	ADJ
afs-72416	86	28	differences	difference	NOUN
afs-72416	86	29	were	be	AUX
afs-72416	86	30	found	find	VERB
afs-72416	86	31	between	between	ADP
afs-72416	86	32	cultivars	cultivar	NOUN
afs-72416	86	33	.	.	PUNCT
afs-72416	87	1	in	in	ADP
afs-72416	87	2	most	most	ADJ
afs-72416	87	3	cases	case	NOUN
afs-72416	87	4	white	white	ADJ
afs-72416	87	5	calli	calli	NOUN
afs-72416	87	6	with	with	ADP
afs-72416	87	7	green	green	ADJ
afs-72416	87	8	or	or	CCONJ
afs-72416	87	9	greenish	greenish	ADJ
afs-72416	87	10	morphogene	morphogene	ADJ
afs-72416	87	11	structures	structure	NOUN
afs-72416	87	12	(	(	PUNCT
afs-72416	87	13	fig	fig	NOUN
afs-72416	87	14	.	.	PUNCT
afs-72416	87	15	2	2	NUM
afs-72416	87	16	,	,	PUNCT
afs-72416	87	17	indicated	indicate	VERB
afs-72416	87	18	by	by	ADP
afs-72416	87	19	arrows	arrow	NOUN
afs-72416	87	20	)	)	PUNCT
afs-72416	87	21	were	be	AUX
afs-72416	87	22	obtained	obtain	VERB
afs-72416	87	23	on	on	ADP
afs-72416	87	24	the	the	DET
afs-72416	87	25	regeneration	regeneration	NOUN
afs-72416	87	26	medium	medium	NOUN
afs-72416	87	27	but	but	CCONJ
afs-72416	87	28	the	the	DET
afs-72416	87	29	efficiency	efficiency	NOUN
afs-72416	87	30	of	of	ADP
afs-72416	87	31	plant	plant	NOUN
afs-72416	87	32	regeneration	regeneration	NOUN
afs-72416	87	33	remained	remain	VERB
afs-72416	87	34	low	low	ADJ
afs-72416	87	35	(	(	PUNCT
afs-72416	87	36	table	table	NOUN
afs-72416	87	37	3	3	NUM
afs-72416	87	38	)	)	PUNCT
afs-72416	87	39	.	.	PUNCT
afs-72416	88	1	initially	initially	ADV
afs-72416	88	2	,	,	PUNCT
afs-72416	88	3	only	only	ADV
afs-72416	88	4	in	in	ADP
afs-72416	88	5	ten	ten	NUM
afs-72416	88	6	of	of	ADP
afs-72416	88	7	fourteen	fourteen	NUM
afs-72416	88	8	brassica	brassica	NOUN
afs-72416	88	9	genotypes	genotype	NOUN
afs-72416	88	10	plant	plant	NOUN
afs-72416	88	11	regeneration	regeneration	NOUN
afs-72416	88	12	was	be	AUX
afs-72416	88	13	successful	successful	ADJ
afs-72416	88	14	.	.	PUNCT
afs-72416	89	1	the	the	DET
afs-72416	89	2	regenerants	regenerant	NOUN
afs-72416	89	3	(	(	PUNCT
afs-72416	89	4	fig	fig	NOUN
afs-72416	89	5	.	.	PUNCT
afs-72416	90	1	3	3	X
afs-72416	90	2	)	)	PUNCT
afs-72416	90	3	were	be	AUX
afs-72416	90	4	obtained	obtain	VERB
afs-72416	90	5	from	from	ADP
afs-72416	90	6	green	green	ADJ
afs-72416	90	7	organogenetic	organogenetic	ADJ
afs-72416	90	8	centres	centre	NOUN
afs-72416	90	9	on	on	ADP
afs-72416	90	10	the	the	DET
afs-72416	90	11	surface	surface	NOUN
afs-72416	90	12	of	of	ADP
afs-72416	90	13	white	white	ADJ
afs-72416	90	14	calli	calli	NOUN
afs-72416	90	15	,	,	PUNCT
afs-72416	90	16	and	and	CCONJ
afs-72416	90	17	generally	generally	ADV
afs-72416	90	18	one	one	NUM
afs-72416	90	19	or	or	CCONJ
afs-72416	90	20	two	two	NUM
afs-72416	90	21	shoots	shoot	NOUN
afs-72416	90	22	were	be	AUX
afs-72416	90	23	obtained	obtain	VERB
afs-72416	90	24	from	from	ADP
afs-72416	90	25	one	one	NUM
afs-72416	90	26	callus	callus	NOUN
afs-72416	90	27	(	(	PUNCT
afs-72416	90	28	fig	fig	NOUN
afs-72416	90	29	.	.	PUNCT
afs-72416	91	1	3	3	NUM
afs-72416	91	2	)	)	PUNCT
afs-72416	91	3	.	.	PUNCT
afs-72416	92	1	consequently	consequently	ADV
afs-72416	92	2	,	,	PUNCT
afs-72416	92	3	to	to	PART
afs-72416	92	4	test	test	VERB
afs-72416	92	5	its	its	PRON
afs-72416	92	6	effect	effect	NOUN
afs-72416	92	7	on	on	ADP
afs-72416	92	8	regeneration	regeneration	NOUN
afs-72416	92	9	,	,	PUNCT
afs-72416	92	10	agnq3	agnq3	NOUN
afs-72416	92	11	was	be	AUX
afs-72416	92	12	added	add	VERB
afs-72416	92	13	to	to	ADP
afs-72416	92	14	the	the	DET
afs-72416	92	15	regeneration	regeneration	NOUN
afs-72416	92	16	medium	medium	NOUN
afs-72416	92	17	.	.	PUNCT
afs-72416	93	1	in	in	ADP
afs-72416	93	2	the	the	DET
afs-72416	93	3	presence	presence	NOUN
afs-72416	93	4	of	of	ADP
afs-72416	93	5	5	5	NUM
afs-72416	93	6	mg	mg	NUM
afs-72416	93	7	/	/	SYM
afs-72416	93	8	i	i	PRON
afs-72416	93	9	agn0	agn0	PROPN
afs-72416	93	10	3	3	NUM
afs-72416	93	11	the	the	DET
afs-72416	93	12	regeneration	regeneration	NOUN
afs-72416	93	13	efficiency	efficiency	NOUN
afs-72416	93	14	was	be	AUX
afs-72416	93	15	substantially	substantially	ADV
afs-72416	93	16	increased	increase	VERB
afs-72416	93	17	including	include	VERB
afs-72416	93	18	multiple	multiple	ADJ
afs-72416	93	19	shoots	shoot	NOUN
afs-72416	93	20	(	(	PUNCT
afs-72416	93	21	fig	fig	NOUN
afs-72416	93	22	.	.	NOUN
afs-72416	93	23	4	4	NUM
afs-72416	93	24	and	and	CCONJ
afs-72416	93	25	5	5	NUM
afs-72416	93	26	)	)	PUNCT
afs-72416	93	27	in	in	ADP
afs-72416	93	28	the	the	DET
afs-72416	93	29	positive	positive	ADJ
afs-72416	93	30	cases	case	NOUN
afs-72416	93	31	.	.	PUNCT
afs-72416	94	1	out	out	ADP
afs-72416	94	2	of	of	ADP
afs-72416	94	3	the	the	DET
afs-72416	94	4	fourteen	fourteen	NUM
afs-72416	94	5	brassica	brassica	NOUN
afs-72416	94	6	genotypes	genotype	NOUN
afs-72416	94	7	studied	study	VERB
afs-72416	94	8	,	,	PUNCT
afs-72416	94	9	thirteen	thirteen	NUM
afs-72416	94	10	gave	give	VERB
afs-72416	94	11	plantlets	plantlet	NOUN
afs-72416	94	12	with	with	ADP
afs-72416	94	13	varying	vary	VERB
afs-72416	94	14	numbers	number	NOUN
afs-72416	94	15	of	of	ADP
afs-72416	94	16	regenerants	regenerant	NOUN
afs-72416	94	17	(	(	PUNCT
afs-72416	94	18	table	table	NOUN
afs-72416	94	19	3	3	NUM
afs-72416	94	20	)	)	PUNCT
afs-72416	94	21	.	.	PUNCT
afs-72416	95	1	this	this	DET
afs-72416	95	2	modified	modify	VERB
afs-72416	95	3	regeneration	regeneration	NOUN
afs-72416	95	4	procedure	procedure	NOUN
afs-72416	95	5	re374	re374	NOUN
afs-72416	95	6	375	375	NUM
afs-72416	95	7	table	table	NOUN
afs-72416	95	8	2	2	NUM
afs-72416	95	9	.	.	PUNCT
afs-72416	95	10	summary	summary	NOUN
afs-72416	95	11	of	of	ADP
afs-72416	95	12	results	result	NOUN
afs-72416	95	13	in	in	ADP
afs-72416	95	14	protoplast	protoplast	NOUN
afs-72416	95	15	isolation	isolation	NOUN
afs-72416	95	16	,	,	PUNCT
afs-72416	95	17	culture	culture	NOUN
afs-72416	95	18	,	,	PUNCT
afs-72416	95	19	callus	callus	ADJ
afs-72416	95	20	induction	induction	NOUN
afs-72416	95	21	,	,	PUNCT
afs-72416	95	22	plant	plant	NOUN
afs-72416	95	23	regeneration	regeneration	NOUN
afs-72416	95	24	and	and	CCONJ
afs-72416	95	25	source	source	NOUN
afs-72416	95	26	of	of	ADP
afs-72416	95	27	genotypes	genotype	NOUN
afs-72416	95	28	used	use	VERB
afs-72416	95	29	in	in	ADP
afs-72416	95	30	investigations	investigation	NOUN
afs-72416	95	31	.	.	PUNCT
afs-72416	96	1	species	specie	NOUN
afs-72416	96	2	,	,	PUNCT
afs-72416	96	3	variety	variety	NOUN
afs-72416	96	4	and	and	CCONJ
afs-72416	96	5	life	life	NOUN
afs-72416	96	6	form	form	NOUN
afs-72416	96	7	protoplast	protoplast	NOUN
afs-72416	96	8	plant	plant	NOUN
afs-72416	96	9	regenesource	regenesource	NOUN
afs-72416	96	10	~	~	PUNCT
afs-72416	96	11	'	'	PUNCT
afs-72416	96	12	;	;	PUNCT
afs-72416	96	13	i	i	PRON
afs-72416	96	14	'	'	PUNCT
afs-72416	96	15	;	;	PUNCT
afs-72416	96	16	"	"	PUNCT
afs-72416	96	17	~	~	PUNCT
afs-72416	96	18	ration	ration	NOUN
afs-72416	96	19	isolation	isolation	NOUN
afs-72416	96	20	culture	culture	NOUN
afs-72416	96	21	derived	derive	VERB
afs-72416	96	22	calli	calli	PROPN
afs-72416	96	23	b.	b.	PROPN
afs-72416	97	1	napus	napus	PROPN
afs-72416	97	2	olga	olga	PROPN
afs-72416	97	3	s	s	PROPN
afs-72416	97	4	spring	spring	NOUN
afs-72416	97	5	>	>	X
afs-72416	97	6	varma	varma	PROPN
afs-72416	97	7	sf	sf	PROPN
afs-72416	97	8	»	»	PUNCT
afs-72416	97	9	>	>	X
afs-72416	97	10	westar	westar	PROPN
afs-72416	97	11	can	can	AUX
afs-72416	97	12	»	»	VERB
afs-72416	97	13	>	>	X
afs-72416	97	14	kunto	kunto	PROPN
afs-72416	97	15	s	s	PROPN
afs-72416	97	16	»	»	X
afs-72416	97	17	>	>	X
afs-72416	97	18	hanna	hanna	PROPN
afs-72416	97	19	s	s	PART
afs-72416	97	20	»	»	X
afs-72416	97	21	>	>	X
afs-72416	97	22	omega	omega	NOUN
afs-72416	97	23	s	s	X
afs-72416	97	24	»	»	X
afs-72416	97	25	>	>	X
afs-72416	97	26	•	•	PROPN
afs-72416	97	27	>	>	X
afs-72416	97	28	viking	vike	VERB
afs-72416	97	29	dk	dk	PROPN
afs-72416	97	30	winter	winter	PROPN
afs-72416	97	31	>	>	PUNCT
afs-72416	97	32	arabella	arabella	PROPN
afs-72416	97	33	d	d	X
afs-72416	97	34	»	»	X
afs-72416	97	35	>	>	X
afs-72416	97	36	darmor	darmor	NOUN
afs-72416	97	37	f	f	PROPN
afs-72416	97	38	»	»	X
afs-72416	97	39	>	>	X
afs-72416	97	40	santana	santana	PROPN
afs-72416	98	1	d	d	X
afs-72416	98	2	»	»	X
afs-72416	98	3	>	>	X
afs-72416	98	4	danubia	danubia	PROPN
afs-72416	98	5	h	h	NOUN
afs-72416	98	6	»	»	PUNCT
afs-72416	98	7	>	>	X
afs-72416	98	8	>	>	X
afs-72416	99	1	belinda	belinda	PROPN
afs-72416	99	2	d	d	PROPN
afs-72416	99	3	»	»	X
afs-72416	99	4	>	>	X
afs-72416	99	5	ujfertddi	ujfertddi	NOUN
afs-72416	99	6	h	h	NOUN
afs-72416	99	7	»	»	PUNCT
afs-72416	99	8	>	>	X
afs-72416	99	9	b.	b.	PROPN
afs-72416	99	10	campestris	campestris	PROPN
afs-72416	99	11	jo	jo	PROPN
afs-72416	99	12	.	.	PROPN
afs-72416	99	13	4001	4001	NUM
afs-72416	100	1	sf	sf	PROPN
afs-72416	100	2	spring	spring	NOUN
afs-72416	100	3	>	>	X
afs-72416	100	4	•	•	ADP
afs-72416	100	5	>	>	X
afs-72416	100	6	>	>	X
afs-72416	100	7	without	without	ADP
afs-72416	100	8	silver	silver	ADJ
afs-72416	100	9	nitrate	nitrate	NOUN
afs-72416	100	10	in	in	ADP
afs-72416	100	11	regeneration	regeneration	NOUN
afs-72416	100	12	medium	medium	NOUN
afs-72416	100	13	■	■	PROPN
afs-72416	100	14	>	>	X
afs-72416	100	15	with	with	ADP
afs-72416	100	16	silver	silver	ADJ
afs-72416	100	17	nitrate	nitrate	NOUN
afs-72416	100	18	in	in	ADP
afs-72416	100	19	regeneration	regeneration	NOUN
afs-72416	100	20	medium	medium	ADJ
afs-72416	100	21	generation	generation	NOUN
afs-72416	100	22	medium	medium	NOUN
afs-72416	100	23	supplemented	supplement	VERB
afs-72416	100	24	with	with	ADP
afs-72416	100	25	agng3	agng3	PROPN
afs-72416	100	26	has	have	VERB
afs-72416	100	27	a	a	DET
afs-72416	100	28	particularly	particularly	ADV
afs-72416	100	29	advantageous	advantageous	ADJ
afs-72416	100	30	effect	effect	NOUN
afs-72416	100	31	on	on	ADP
afs-72416	100	32	regeneration	regeneration	NOUN
afs-72416	100	33	of	of	ADP
afs-72416	100	34	b.	b.	PROPN
afs-72416	100	35	campestris	campestris	PROPN
afs-72416	100	36	which	which	PRON
afs-72416	100	37	has	have	AUX
afs-72416	100	38	been	be	AUX
afs-72416	100	39	table	table	NOUN
afs-72416	100	40	3	3	NUM
afs-72416	100	41	.	.	PUNCT
afs-72416	101	1	the	the	DET
afs-72416	101	2	effect	effect	NOUN
afs-72416	101	3	of	of	ADP
afs-72416	101	4	silvernitrate	silvernitrate	NOUN
afs-72416	101	5	(	(	PUNCT
afs-72416	101	6	agno	agno	NOUN
afs-72416	101	7	,	,	PUNCT
afs-72416	101	8	)	)	PUNCT
afs-72416	101	9	on	on	ADP
afs-72416	101	10	plant	plant	NOUN
afs-72416	101	11	regeneration	regeneration	NOUN
afs-72416	101	12	from	from	ADP
afs-72416	101	13	protoplastderived	protoplastderive	VERB
afs-72416	101	14	calli	calli	NOUN
afs-72416	101	15	of	of	ADP
afs-72416	101	16	b.	b.	PROPN
afs-72416	101	17	napus	napus	PROPN
afs-72416	101	18	and	and	CCONJ
afs-72416	101	19	b.	b.	PROPN
afs-72416	101	20	campestris	campestris	PROPN
afs-72416	101	21	varieties	variety	NOUN
afs-72416	101	22	.	.	PUNCT
afs-72416	102	1	name	name	NOUN
afs-72416	102	2	of	of	ADP
afs-72416	102	3	genotype	genotype	NOUN
afs-72416	102	4	%	%	NOUN
afs-72416	102	5	of	of	ADP
afs-72416	102	6	shoot	shoot	NOUN
afs-72416	102	7	regenerating	regenerating	NOUN
afs-72416	102	8	and	and	CCONJ
afs-72416	102	9	life	life	NOUN
afs-72416	102	10	form	form	NOUN
afs-72416	102	11	calli	calli	NOUN
afs-72416	102	12	—	—	PUNCT
afs-72416	102	13	agno	agno	NOUN
afs-72416	102	14	,	,	PUNCT
afs-72416	102	15	+	+	CCONJ
afs-72416	102	16	agno	agno	NOUN
afs-72416	102	17	,	,	PUNCT
afs-72416	102	18	b.	b.	PROPN
afs-72416	102	19	napus	napus	PROPN
afs-72416	102	20	olga	olga	PROPN
afs-72416	102	21	*	*	PUNCT
afs-72416	102	22	s.	s.	PROPN
afs-72416	102	23	24	24	NUM
afs-72416	102	24	41	41	NUM
afs-72416	102	25	varma	varma	PROPN
afs-72416	102	26	s.	s.	PROPN
afs-72416	102	27	31	31	NUM
afs-72416	103	1	39	39	NUM
afs-72416	103	2	westar	westar	PROPN
afs-72416	103	3	s.	s.	PROPN
afs-72416	103	4	19	19	NUM
afs-72416	103	5	33	33	NUM
afs-72416	103	6	kunto	kunto	NOUN
afs-72416	103	7	s.	s.	PROPN
afs-72416	103	8	11	11	NUM
afs-72416	103	9	29	29	NUM
afs-72416	103	10	hanna	hanna	PROPN
afs-72416	103	11	s.	s.	PROPN
afs-72416	103	12	9	9	NUM
afs-72416	103	13	19	19	NUM
afs-72416	103	14	omega	omega	NOUN
afs-72416	103	15	s.	s.	PROPN
afs-72416	103	16	0	0	PROPN
afs-72416	103	17	4	4	NUM
afs-72416	103	18	viking	vike	VERB
afs-72416	103	19	*	*	PROPN
afs-72416	103	20	*	*	PUNCT
afs-72416	103	21	w.	w.	NOUN
afs-72416	103	22	9	9	NUM
afs-72416	103	23	23	23	NUM
afs-72416	103	24	arabella	arabella	ADP
afs-72416	103	25	w.	w.	PROPN
afs-72416	103	26	10	10	NUM
afs-72416	103	27	17	17	NUM
afs-72416	103	28	darmor	darmor	NOUN
afs-72416	103	29	w.	w.	PROPN
afs-72416	103	30	4	4	NUM
afs-72416	103	31	14	14	NUM
afs-72416	103	32	santana	santana	PROPN
afs-72416	103	33	w.	w.	PROPN
afs-72416	103	34	6	6	NUM
afs-72416	103	35	10	10	NUM
afs-72416	103	36	danubia	danubia	PROPN
afs-72416	103	37	w.	w.	PROPN
afs-72416	103	38	0	0	PROPN
afs-72416	103	39	8	8	PROPN
afs-72416	103	40	belinda	belinda	PROPN
afs-72416	103	41	w.	w.	PROPN
afs-72416	103	42	2	2	NUM
afs-72416	103	43	6	6	NUM
afs-72416	103	44	ujfertddi	ujfertddi	NOUN
afs-72416	103	45	w.	w.	NOUN
afs-72416	103	46	0	0	PROPN
afs-72416	103	47	0	0	PROPN
afs-72416	103	48	b.	b.	PROPN
afs-72416	103	49	campestris	campestris	PROPN
afs-72416	103	50	jo	jo	PROPN
afs-72416	103	51	.	.	PROPN
afs-72416	103	52	4001	4001	NUM
afs-72416	104	1	s.	s.	PROPN
afs-72416	104	2	0	0	NUM
afs-72416	105	1	22	22	NUM
afs-72416	105	2	•	•	NUM
afs-72416	105	3	s	s	PART
afs-72416	105	4	=	=	NOUN
afs-72416	105	5	spring	spring	NOUN
afs-72416	105	6	,	,	PUNCT
afs-72416	105	7	*	*	PUNCT
afs-72416	105	8	*	*	PUNCT
afs-72416	105	9	w	w	NOUN
afs-72416	105	10	=	=	PUNCT
afs-72416	105	11	winter	winter	NOUN
afs-72416	105	12	regeneration	regeneration	NOUN
afs-72416	105	13	without	without	ADP
afs-72416	105	14	agnoj	agnoj	NOUN
afs-72416	105	15	(	(	PUNCT
afs-72416	105	16	—	—	PUNCT
afs-72416	105	17	)	)	PUNCT
afs-72416	105	18	with	with	ADP
afs-72416	105	19	agn03	agn03	PROPN
afs-72416	105	20	(	(	PUNCT
afs-72416	105	21	+	+	CCONJ
afs-72416	105	22	)	)	PUNCT
afs-72416	105	23	known	know	VERB
afs-72416	105	24	to	to	PART
afs-72416	105	25	respond	respond	VERB
afs-72416	105	26	poorly	poorly	ADV
afs-72416	105	27	to	to	ADP
afs-72416	105	28	in	in	ADP
afs-72416	105	29	vitro	vitro	X
afs-72416	105	30	manipulation	manipulation	NOUN
afs-72416	105	31	.	.	PUNCT
afs-72416	106	1	the	the	DET
afs-72416	106	2	regenerated	regenerated	ADJ
afs-72416	106	3	plantlets	plantlet	NOUN
afs-72416	106	4	were	be	AUX
afs-72416	106	5	transferred	transfer	VERB
afs-72416	106	6	to	to	ADP
afs-72416	106	7	k	k	PROPN
afs-72416	106	8	3	3	NUM
afs-72416	106	9	medium	medium	NOUN
afs-72416	106	10	without	without	ADP
afs-72416	106	11	agn0	agn0	PROPN
afs-72416	106	12	3	3	NUM
afs-72416	106	13	which	which	PRON
afs-72416	106	14	allowed	allow	VERB
afs-72416	106	15	the	the	DET
afs-72416	106	16	plantlets	plantlet	NOUN
afs-72416	106	17	to	to	PART
afs-72416	106	18	develop	develop	VERB
afs-72416	106	19	rapidly	rapidly	ADV
afs-72416	106	20	.	.	PUNCT
afs-72416	107	1	discussion	discussion	NOUN
afs-72416	107	2	the	the	DET
afs-72416	107	3	molarity	molarity	NOUN
afs-72416	107	4	of	of	ADP
afs-72416	107	5	enzyme	enzyme	NOUN
afs-72416	107	6	solution	solution	NOUN
afs-72416	107	7	was	be	AUX
afs-72416	107	8	raised	raise	VERB
afs-72416	107	9	from	from	ADP
afs-72416	107	10	0,4	0,4	NUM
afs-72416	107	11	m	m	NOUN
afs-72416	107	12	sucrose	sucrose	NOUN
afs-72416	107	13	suggested	suggest	VERB
afs-72416	107	14	by	by	ADP
afs-72416	107	15	glimelius	glimelius	NOUN
afs-72416	107	16	(	(	PUNCT
afs-72416	107	17	1984	1984	NUM
afs-72416	107	18	)	)	PUNCT
afs-72416	107	19	and	and	CCONJ
afs-72416	107	20	vamling	vamling	NOUN
afs-72416	107	21	and	and	CCONJ
afs-72416	107	22	glimelius	glimelius	NOUN
afs-72416	107	23	(	(	PUNCT
afs-72416	107	24	1990	1990	NUM
afs-72416	107	25	)	)	PUNCT
afs-72416	107	26	to	to	PART
afs-72416	107	27	fig	fig	VERB
afs-72416	107	28	.	.	PUNCT
afs-72416	108	1	2	2	X
afs-72416	108	2	.	.	X
afs-72416	108	3	protoplast	protoplast	NOUN
afs-72416	108	4	-	-	PUNCT
afs-72416	108	5	derived	derive	VERB
afs-72416	108	6	calli	calli	NOUN
afs-72416	108	7	with	with	ADP
afs-72416	108	8	morphogene	morphogene	ADJ
afs-72416	108	9	structure	structure	NOUN
afs-72416	108	10	on	on	ADP
afs-72416	108	11	their	their	PRON
afs-72416	108	12	surface	surface	NOUN
afs-72416	108	13	(	(	PUNCT
afs-72416	108	14	arrows	arrow	NOUN
afs-72416	108	15	)	)	PUNCT
afs-72416	108	16	on	on	ADP
afs-72416	108	17	plant	plant	NOUN
afs-72416	108	18	regeneration	regeneration	NOUN
afs-72416	108	19	medium	medium	NOUN
afs-72416	108	20	without	without	ADP
afs-72416	108	21	silver	silver	NOUN
afs-72416	108	22	nitrate	nitrate	NOUN
afs-72416	108	23	.	.	PUNCT
afs-72416	109	1	0,714	0,714	NUM
afs-72416	109	2	m	m	NUM
afs-72416	109	3	mannitol	mannitol	NOUN
afs-72416	109	4	.	.	PUNCT
afs-72416	110	1	we	we	PRON
afs-72416	110	2	thus	thus	ADV
afs-72416	110	3	used	use	VERB
afs-72416	110	4	higher	high	ADJ
afs-72416	110	5	molarity	molarity	NOUN
afs-72416	110	6	as	as	ADP
afs-72416	110	7	kohlenbach	kohlenbach	NOUN
afs-72416	110	8	et	et	PROPN
afs-72416	110	9	al	al	PROPN
afs-72416	110	10	.	.	PROPN
afs-72416	111	1	(	(	PUNCT
afs-72416	111	2	1982	1982	NUM
afs-72416	111	3	)	)	PUNCT
afs-72416	111	4	who	who	PRON
afs-72416	111	5	used	use	VERB
afs-72416	111	6	0,55	0,55	NOUN
afs-72416	111	7	m	m	VERB
afs-72416	111	8	and	and	CCONJ
afs-72416	111	9	0,65	0,65	NUM
afs-72416	111	10	m	m	NOUN
afs-72416	111	11	mannitol	mannitol	NOUN
afs-72416	111	12	for	for	ADP
afs-72416	111	13	leaf	leaf	NOUN
afs-72416	111	14	and	and	CCONJ
afs-72416	111	15	stem	stem	VERB
afs-72416	111	16	embryo	embryo	NOUN
afs-72416	111	17	protoplast	protoplast	NOUN
afs-72416	111	18	isolation	isolation	NOUN
afs-72416	111	19	,	,	PUNCT
afs-72416	111	20	respectively	respectively	ADV
afs-72416	111	21	.	.	PUNCT
afs-72416	112	1	eapen	eapen	PROPN
afs-72416	112	2	et	et	PROPN
afs-72416	112	3	al	al	PROPN
afs-72416	112	4	.	.	PROPN
afs-72416	113	1	(	(	PUNCT
afs-72416	113	2	1989	1989	NUM
afs-72416	113	3	)	)	PUNCT
afs-72416	113	4	obtained	obtain	VERB
afs-72416	113	5	good	good	ADJ
afs-72416	113	6	results	result	NOUN
afs-72416	113	7	using	use	VERB
afs-72416	113	8	0,6	0,6	NUM
afs-72416	113	9	m	m	NOUN
afs-72416	113	10	mannitol	mannitol	NOUN
afs-72416	113	11	in	in	ADP
afs-72416	113	12	enzyme	enzyme	NOUN
afs-72416	113	13	solution	solution	NOUN
afs-72416	113	14	in	in	ADP
afs-72416	113	15	mesophyll	mesophyll	NOUN
afs-72416	113	16	protoplast	protoplast	NOUN
afs-72416	113	17	isolation	isolation	NOUN
afs-72416	113	18	of	of	ADP
afs-72416	113	19	brassica	brassica	PROPN
afs-72416	113	20	juncea	juncea	PROPN
afs-72416	113	21	.	.	PUNCT
afs-72416	114	1	the	the	DET
afs-72416	114	2	cytoplasm	cytoplasm	ADJ
afs-72416	114	3	dense	dense	ADJ
afs-72416	114	4	smallprotoplasts	smallprotoplast	NOUN
afs-72416	114	5	proved	prove	VERB
afs-72416	114	6	to	to	PART
afs-72416	114	7	be	be	AUX
afs-72416	114	8	well	well	ADV
afs-72416	114	9	manipulable	manipulable	ADJ
afs-72416	114	10	during	during	ADP
afs-72416	114	11	isolation	isolation	NOUN
afs-72416	114	12	procedure	procedure	NOUN
afs-72416	114	13	.	.	PUNCT
afs-72416	115	1	they	they	PRON
afs-72416	115	2	will	will	AUX
afs-72416	115	3	be	be	AUX
afs-72416	115	4	an	an	DET
afs-72416	115	5	essential	essential	ADJ
afs-72416	115	6	advantage	advantage	NOUN
afs-72416	115	7	in	in	ADP
afs-72416	115	8	direct	direct	ADJ
afs-72416	115	9	protoplast	protoplast	NOUN
afs-72416	115	10	transformation	transformation	NOUN
afs-72416	115	11	experiments	experiment	NOUN
afs-72416	115	12	where	where	SCONJ
afs-72416	115	13	protoplasts	protoplast	NOUN
afs-72416	115	14	are	be	AUX
afs-72416	115	15	manipulated	manipulate	VERB
afs-72416	115	16	washing	washing	NOUN
afs-72416	115	17	etc	etc	NOUN
afs-72416	115	18	.	.	PUNCT
afs-72416	116	1	several	several	ADJ
afs-72416	116	2	times	time	NOUN
afs-72416	116	3	.	.	PUNCT
afs-72416	117	1	the	the	DET
afs-72416	117	2	agarose	agarose	NOUN
afs-72416	117	3	embedding	embed	VERB
afs-72416	117	4	culture	culture	NOUN
afs-72416	117	5	technique	technique	NOUN
afs-72416	117	6	developed	develop	VERB
afs-72416	117	7	by	by	ADP
afs-72416	117	8	shillito	shillito	PROPN
afs-72416	117	9	et	et	PROPN
afs-72416	117	10	al	al	PROPN
afs-72416	117	11	.	.	PROPN
afs-72416	117	12	(	(	PUNCT
afs-72416	117	13	1983	1983	NUM
afs-72416	117	14	)	)	PUNCT
afs-72416	117	15	has	have	AUX
afs-72416	117	16	proven	prove	VERB
afs-72416	117	17	to	to	PART
afs-72416	117	18	be	be	AUX
afs-72416	117	19	a	a	DET
afs-72416	117	20	successful	successful	ADJ
afs-72416	117	21	method	method	NOUN
afs-72416	117	22	in	in	ADP
afs-72416	117	23	brassica	brassica	NOUN
afs-72416	117	24	also	also	ADV
afs-72416	117	25	.	.	PUNCT
afs-72416	118	1	our	our	PRON
afs-72416	118	2	results	result	NOUN
afs-72416	118	3	confirmed	confirm	VERB
afs-72416	118	4	the	the	DET
afs-72416	118	5	applicability	applicability	NOUN
afs-72416	118	6	of	of	ADP
afs-72416	118	7	the	the	DET
afs-72416	118	8	embedded	embed	VERB
afs-72416	118	9	protoplast	protoplast	NOUN
afs-72416	118	10	culture	culture	NOUN
afs-72416	118	11	technique	technique	NOUN
afs-72416	118	12	(	(	PUNCT
afs-72416	118	13	directly	directly	ADV
afs-72416	118	14	or	or	CCONJ
afs-72416	118	15	on	on	ADP
afs-72416	118	16	the	the	DET
afs-72416	118	17	2nd	2nd	ADJ
afs-72416	118	18	day	day	NOUN
afs-72416	118	19	of	of	ADP
afs-72416	118	20	subculturing	subculturing	NOUN
afs-72416	118	21	)	)	PUNCT
afs-72416	118	22	which	which	PRON
afs-72416	118	23	produced	produce	VERB
afs-72416	118	24	the	the	DET
afs-72416	118	25	same	same	ADJ
afs-72416	118	26	division	division	NOUN
afs-72416	118	27	results	result	NOUN
afs-72416	118	28	as	as	ADP
afs-72416	118	29	the	the	DET
afs-72416	118	30	liquid	liquid	ADJ
afs-72416	118	31	culture	culture	NOUN
afs-72416	118	32	method	method	NOUN
afs-72416	118	33	(	(	PUNCT
afs-72416	118	34	basic	basic	ADJ
afs-72416	118	35	culture	culture	NOUN
afs-72416	118	36	method	method	NOUN
afs-72416	118	37	)	)	PUNCT
afs-72416	118	38	published	publish	VERB
afs-72416	118	39	for	for	ADP
afs-72416	118	40	brassica	brassica	NOUN
afs-72416	118	41	protoplast	protoplast	NOUN
afs-72416	118	42	culture	culture	NOUN
afs-72416	118	43	(	(	PUNCT
afs-72416	118	44	bidney	bidney	NOUN
afs-72416	118	45	et	et	PROPN
afs-72416	118	46	al	al	PROPN
afs-72416	118	47	.	.	PROPN
afs-72416	118	48	1983	1983	NUM
afs-72416	118	49	,	,	PUNCT
afs-72416	118	50	glimelius	glimelius	NOUN
afs-72416	118	51	1984	1984	NUM
afs-72416	118	52	,	,	PUNCT
afs-72416	118	53	jourdan	jourdan	PROPN
afs-72416	118	54	et	et	PROPN
afs-72416	118	55	al	al	PROPN
afs-72416	118	56	.	.	PROPN
afs-72416	118	57	1989	1989	NUM
afs-72416	118	58	,	,	PUNCT
afs-72416	118	59	loudon	loudon	PROPN
afs-72416	118	60	et	et	PROPN
afs-72416	118	61	al	al	PROPN
afs-72416	118	62	.	.	PROPN
afs-72416	118	63	1989	1989	NUM
afs-72416	118	64	)	)	PUNCT
afs-72416	118	65	.	.	PUNCT
afs-72416	119	1	this	this	DET
afs-72416	119	2	technique	technique	NOUN
afs-72416	119	3	has	have	AUX
afs-72416	119	4	been	be	AUX
afs-72416	119	5	already	already	ADV
afs-72416	119	6	used	use	VERB
afs-72416	119	7	for	for	ADP
afs-72416	119	8	2—3week	2—3week	NUM
afs-72416	119	9	old	old	ADJ
afs-72416	119	10	colony	colony	NOUN
afs-72416	119	11	embedding	embed	VERB
afs-72416	119	12	by	by	ADP
afs-72416	119	13	vamling	vamle	VERB
afs-72416	119	14	and	and	CCONJ
afs-72416	119	15	glimelius	glimelius	NOUN
afs-72416	119	16	(	(	PUNCT
afs-72416	119	17	1990	1990	NUM
afs-72416	119	18	)	)	PUNCT
afs-72416	119	19	resulting	result	VERB
afs-72416	119	20	in	in	ADP
afs-72416	119	21	a	a	DET
afs-72416	119	22	good	good	ADJ
afs-72416	119	23	culture	culture	NOUN
afs-72416	119	24	practice	practice	NOUN
afs-72416	119	25	,	,	PUNCT
afs-72416	119	26	but	but	CCONJ
afs-72416	119	27	our	our	PRON
afs-72416	119	28	results	result	NOUN
afs-72416	119	29	(	(	PUNCT
afs-72416	119	30	early	early	ADV
afs-72416	119	31	embedding	embed	VERB
afs-72416	119	32	after	after	ADP
afs-72416	119	33	isolation	isolation	NOUN
afs-72416	119	34	)	)	PUNCT
afs-72416	119	35	show	show	VERB
afs-72416	119	36	other	other	ADJ
afs-72416	119	37	advantages	advantage	NOUN
afs-72416	119	38	over	over	ADP
afs-72416	119	39	this	this	DET
afs-72416	119	40	culture	culture	NOUN
afs-72416	119	41	technique	technique	NOUN
afs-72416	119	42	,	,	PUNCT
afs-72416	119	43	too	too	ADV
afs-72416	119	44	.	.	PUNCT
afs-72416	120	1	the	the	DET
afs-72416	120	2	colony	colony	NOUN
afs-72416	120	3	browning	browning	NOUN
afs-72416	120	4	,	,	PUNCT
afs-72416	120	5	fig	fig	NOUN
afs-72416	120	6	.	.	PUNCT
afs-72416	121	1	s.	s.	PROPN
afs-72416	121	2	typical	typical	ADJ
afs-72416	121	3	rapeseed	rapeseed	PROPN
afs-72416	121	4	white	white	ADJ
afs-72416	121	5	callus	callus	NOUN
afs-72416	121	6	with	with	ADP
afs-72416	121	7	one	one	NUM
afs-72416	121	8	shoot	shoot	NOUN
afs-72416	121	9	on	on	ADP
afs-72416	121	10	regeneration	regeneration	NOUN
afs-72416	121	11	medium	medium	NOUN
afs-72416	121	12	without	without	ADP
afs-72416	121	13	silver	silver	ADJ
afs-72416	121	14	nitrate	nitrate	NOUN
afs-72416	121	15	.	.	PUNCT
afs-72416	122	1	fig	fig	NOUN
afs-72416	122	2	.	.	PUNCT
afs-72416	123	1	5	5	NUM
afs-72416	123	2	.	.	X
afs-72416	123	3	one	one	NUM
afs-72416	123	4	shootlet	shootlet	NOUN
afs-72416	123	5	on	on	ADP
afs-72416	123	6	surface	surface	NOUN
afs-72416	123	7	of	of	ADP
afs-72416	123	8	green	green	ADJ
afs-72416	123	9	callus	callus	ADJ
afs-72416	123	10	developing	develop	VERB
afs-72416	123	11	on	on	ADP
afs-72416	123	12	agnoi	agnoi	ADJ
afs-72416	123	13	supplemented	supplement	VERB
afs-72416	123	14	regeneration	regeneration	NOUN
afs-72416	123	15	medium	medium	NOUN
afs-72416	123	16	.	.	PUNCT
afs-72416	124	1	fig	fig	NOUN
afs-72416	124	2	.	.	PUNCT
afs-72416	125	1	4	4	X
afs-72416	125	2	.	.	X
afs-72416	125	3	well	well	ADV
afs-72416	125	4	differentiated	differentiate	VERB
afs-72416	125	5	green	green	ADJ
afs-72416	125	6	protoplast	protoplast	NOUN
afs-72416	125	7	-	-	PUNCT
afs-72416	125	8	derived	derive	VERB
afs-72416	125	9	callus	callus	NOUN
afs-72416	125	10	with	with	ADP
afs-72416	125	11	multiple	multiple	ADJ
afs-72416	125	12	shoot	shoot	NOUN
afs-72416	125	13	primordia	primordium	NOUN
afs-72416	125	14	(	(	PUNCT
afs-72416	125	15	arrows	arrow	NOUN
afs-72416	125	16	)	)	PUNCT
afs-72416	125	17	on	on	ADP
afs-72416	125	18	regeneration	regeneration	NOUN
afs-72416	125	19	medium	medium	NOUN
afs-72416	125	20	supplemented	supplement	VERB
afs-72416	125	21	with	with	ADP
afs-72416	125	22	5	5	NUM
afs-72416	125	23	mg/1	mg/1	NOUN
afs-72416	125	24	agn03	agn03	PROPN
afs-72416	125	25	.	.	PUNCT
afs-72416	126	1	376	376	NUM
afs-72416	126	2	widely	widely	ADV
afs-72416	126	3	reported	report	VERB
afs-72416	126	4	to	to	PART
afs-72416	126	5	be	be	AUX
afs-72416	126	6	a	a	DET
afs-72416	126	7	feature	feature	NOUN
afs-72416	126	8	of	of	ADP
afs-72416	126	9	brassica	brassica	NOUN
afs-72416	126	10	protoplast	protoplast	NOUN
afs-72416	126	11	culture	culture	NOUN
afs-72416	126	12	(	(	PUNCT
afs-72416	126	13	xu	xu	INTJ
afs-72416	126	14	et	et	PROPN
afs-72416	126	15	al	al	PROPN
afs-72416	126	16	.	.	PROPN
afs-72416	126	17	1982	1982	NUM
afs-72416	126	18	,	,	PUNCT
afs-72416	126	19	glimelius	glimelius	NOUN
afs-72416	126	20	1984	1984	NUM
afs-72416	126	21	,	,	PUNCT
afs-72416	126	22	loudon	loudon	PROPN
afs-72416	126	23	et	et	PROPN
afs-72416	126	24	al	al	PROPN
afs-72416	126	25	.	.	PROPN
afs-72416	126	26	1989	1989	NUM
afs-72416	126	27	)	)	PUNCT
afs-72416	126	28	was	be	AUX
afs-72416	126	29	prevented	prevent	VERB
afs-72416	126	30	and	and	CCONJ
afs-72416	126	31	healthy	healthy	ADJ
afs-72416	126	32	calli	calli	NOUN
afs-72416	126	33	were	be	AUX
afs-72416	126	34	induced	induce	VERB
afs-72416	126	35	for	for	ADP
afs-72416	126	36	plant	plant	NOUN
afs-72416	126	37	regeneration	regeneration	NOUN
afs-72416	126	38	experiments	experiment	NOUN
afs-72416	126	39	.	.	PUNCT
afs-72416	127	1	one	one	NUM
afs-72416	127	2	of	of	ADP
afs-72416	127	3	the	the	DET
afs-72416	127	4	noticeable	noticeable	ADJ
afs-72416	127	5	features	feature	NOUN
afs-72416	127	6	of	of	ADP
afs-72416	127	7	this	this	DET
afs-72416	127	8	study	study	NOUN
afs-72416	127	9	is	be	AUX
afs-72416	127	10	the	the	DET
afs-72416	127	11	wide	wide	ADJ
afs-72416	127	12	range	range	NOUN
afs-72416	127	13	of	of	ADP
afs-72416	127	14	responses	response	NOUN
afs-72416	127	15	observed	observe	VERB
afs-72416	127	16	(	(	PUNCT
afs-72416	127	17	table	table	NOUN
afs-72416	127	18	2	2	NUM
afs-72416	127	19	)	)	PUNCT
afs-72416	127	20	.	.	PUNCT
afs-72416	128	1	these	these	DET
afs-72416	128	2	results	result	NOUN
afs-72416	128	3	show	show	VERB
afs-72416	128	4	,	,	PUNCT
afs-72416	128	5	in	in	ADP
afs-72416	128	6	accordance	accordance	NOUN
afs-72416	128	7	with	with	ADP
afs-72416	128	8	other	other	ADJ
afs-72416	128	9	studies	study	NOUN
afs-72416	128	10	(	(	PUNCT
afs-72416	128	11	jourdan	jourdan	PROPN
afs-72416	128	12	and	and	CCONJ
afs-72416	128	13	earle	earle	PROPN
afs-72416	128	14	1989	1989	NUM
afs-72416	128	15	,	,	PUNCT
afs-72416	128	16	loudon	loudon	PROPN
afs-72416	128	17	et	et	PROPN
afs-72416	128	18	al	al	PROPN
afs-72416	128	19	.	.	PROPN
afs-72416	128	20	1989	1989	NUM
afs-72416	128	21	)	)	PUNCT
afs-72416	128	22	that	that	SCONJ
afs-72416	128	23	various	various	ADJ
afs-72416	128	24	brassica	brassica	NOUN
afs-72416	128	25	varieties	variety	NOUN
afs-72416	128	26	of	of	ADP
afs-72416	128	27	great	great	ADJ
afs-72416	128	28	economic	economic	ADJ
afs-72416	128	29	importance	importance	NOUN
afs-72416	128	30	can	can	AUX
afs-72416	128	31	be	be	AUX
afs-72416	128	32	successfully	successfully	ADV
afs-72416	128	33	regenerated	regenerate	VERB
afs-72416	128	34	from	from	ADP
afs-72416	128	35	protoplasts	protoplast	NOUN
afs-72416	128	36	.	.	PUNCT
afs-72416	129	1	there	there	PRON
afs-72416	129	2	was	be	VERB
afs-72416	129	3	only	only	ADV
afs-72416	129	4	one	one	NUM
afs-72416	129	5	(	(	PUNCT
afs-72416	129	6	ujfertodi	ujfertodi	ADV
afs-72416	129	7	)	)	PUNCT
afs-72416	129	8	out	out	ADP
afs-72416	129	9	of	of	ADP
afs-72416	129	10	the	the	DET
afs-72416	129	11	fourteen	fourteen	NUM
afs-72416	129	12	varieties	variety	NOUN
afs-72416	129	13	tested	test	VERB
afs-72416	129	14	that	that	PRON
afs-72416	129	15	failed	fail	VERB
afs-72416	129	16	to	to	PART
afs-72416	129	17	produce	produce	VERB
afs-72416	129	18	plantlets	plantlet	NOUN
afs-72416	129	19	.	.	PUNCT
afs-72416	130	1	incorporation	incorporation	NOUN
afs-72416	130	2	of	of	ADP
afs-72416	130	3	silver	silver	ADJ
afs-72416	130	4	nitrate	nitrate	NOUN
afs-72416	130	5	(	(	PUNCT
afs-72416	130	6	5	5	NUM
afs-72416	130	7	mg/1	mg/1	NOUN
afs-72416	130	8	)	)	PUNCT
afs-72416	130	9	into	into	ADP
afs-72416	130	10	the	the	DET
afs-72416	130	11	regeneration	regeneration	NOUN
afs-72416	130	12	medium	medium	NOUN
afs-72416	130	13	improved	improve	VERB
afs-72416	130	14	the	the	DET
afs-72416	130	15	efficiency	efficiency	NOUN
afs-72416	130	16	of	of	ADP
afs-72416	130	17	plant	plant	NOUN
afs-72416	130	18	regeneration	regeneration	NOUN
afs-72416	130	19	.	.	PUNCT
afs-72416	131	1	with	with	ADP
afs-72416	131	2	the	the	DET
afs-72416	131	3	introduction	introduction	NOUN
afs-72416	131	4	of	of	ADP
afs-72416	131	5	agnoj	agnoj	NOUN
afs-72416	131	6	three	three	NUM
afs-72416	131	7	of	of	ADP
afs-72416	131	8	the	the	DET
afs-72416	131	9	examined	examine	VERB
afs-72416	131	10	varieties	variety	NOUN
afs-72416	131	11	became	become	VERB
afs-72416	131	12	responsive	responsive	ADJ
afs-72416	131	13	in	in	ADP
afs-72416	131	14	regeneration	regeneration	NOUN
afs-72416	131	15	experiments	experiment	NOUN
afs-72416	131	16	(	(	PUNCT
afs-72416	131	17	table	table	NOUN
afs-72416	131	18	3	3	NUM
afs-72416	131	19	)	)	PUNCT
afs-72416	131	20	and	and	CCONJ
afs-72416	131	21	regeneration	regeneration	NOUN
afs-72416	131	22	was	be	AUX
afs-72416	131	23	more	more	ADV
afs-72416	131	24	efficient	efficient	ADJ
afs-72416	131	25	in	in	ADP
afs-72416	131	26	each	each	DET
afs-72416	131	27	variety	variety	NOUN
afs-72416	131	28	.	.	PUNCT
afs-72416	132	1	there	there	PRON
afs-72416	132	2	is	be	VERB
afs-72416	132	3	only	only	ADV
afs-72416	132	4	one	one	NUM
afs-72416	132	5	recalcitrant	recalcitrant	ADJ
afs-72416	132	6	genotype	genotype	NOUN
afs-72416	132	7	ujfertodi	ujfertodi	ADV
afs-72416	132	8	in	in	ADP
afs-72416	132	9	our	our	PRON
afs-72416	132	10	experiments	experiment	NOUN
afs-72416	132	11	(	(	PUNCT
afs-72416	132	12	table	table	NOUN
afs-72416	132	13	2	2	NUM
afs-72416	132	14	and	and	CCONJ
afs-72416	132	15	3	3	NUM
afs-72416	132	16	)	)	PUNCT
afs-72416	132	17	.	.	PUNCT
afs-72416	133	1	in	in	ADP
afs-72416	133	2	agreement	agreement	NOUN
afs-72416	133	3	with	with	ADP
afs-72416	133	4	our	our	PRON
afs-72416	133	5	results	result	NOUN
afs-72416	133	6	,	,	PUNCT
afs-72416	133	7	the	the	DET
afs-72416	133	8	positive	positive	ADJ
afs-72416	133	9	effect	effect	NOUN
afs-72416	133	10	of	of	ADP
afs-72416	133	11	agnq	agnq	NOUN
afs-72416	133	12	3	3	NUM
afs-72416	133	13	on	on	ADP
afs-72416	133	14	the	the	DET
afs-72416	133	15	induction	induction	NOUN
afs-72416	133	16	of	of	ADP
afs-72416	133	17	plant	plant	NOUN
afs-72416	133	18	regeneration	regeneration	NOUN
afs-72416	133	19	has	have	AUX
afs-72416	133	20	been	be	AUX
afs-72416	133	21	recently	recently	ADV
afs-72416	133	22	published	publish	VERB
afs-72416	133	23	by	by	ADP
afs-72416	133	24	other	other	ADJ
afs-72416	133	25	authors	author	NOUN
afs-72416	133	26	as	as	ADV
afs-72416	133	27	well	well	ADV
afs-72416	133	28	(	(	PUNCT
afs-72416	133	29	lentini	lentini	PROPN
afs-72416	133	30	et	et	PROPN
afs-72416	133	31	al	al	PROPN
afs-72416	133	32	.	.	PROPN
afs-72416	133	33	1988	1988	NUM
afs-72416	133	34	,	,	PUNCT
afs-72416	133	35	songstad	songstad	NOUN
afs-72416	133	36	et	et	PROPN
afs-72416	133	37	al	al	PROPN
afs-72416	133	38	.	.	PROPN
afs-72416	133	39	1988	1988	NUM
afs-72416	133	40	)	)	PUNCT
afs-72416	133	41	in	in	ADP
afs-72416	133	42	brassica	brassica	NOUN
afs-72416	133	43	and	and	CCONJ
afs-72416	133	44	in	in	ADP
afs-72416	133	45	other	other	ADJ
afs-72416	133	46	crop	crop	NOUN
afs-72416	133	47	plants	plant	NOUN
afs-72416	133	48	(	(	PUNCT
afs-72416	133	49	purnhauser	purnhauser	NOUN
afs-72416	133	50	et	et	PROPN
afs-72416	133	51	al	al	PROPN
afs-72416	133	52	.	.	PROPN
afs-72416	133	53	1987	1987	NUM
afs-72416	133	54	)	)	PUNCT
afs-72416	133	55	.	.	PUNCT
afs-72416	134	1	our	our	PRON
afs-72416	134	2	results	result	NOUN
afs-72416	134	3	support	support	VERB
afs-72416	134	4	the	the	DET
afs-72416	134	5	findings	finding	NOUN
afs-72416	134	6	of	of	ADP
afs-72416	134	7	purnhauser	purnhauser	NOUN
afs-72416	134	8	et	et	PROPN
afs-72416	134	9	al	al	PROPN
afs-72416	134	10	.	.	PROPN
afs-72416	135	1	(	(	PUNCT
afs-72416	135	2	1987	1987	NUM
afs-72416	135	3	)	)	PUNCT
afs-72416	135	4	and	and	CCONJ
afs-72416	135	5	williams	williams	PROPN
afs-72416	135	6	et	et	PROPN
afs-72416	135	7	al	al	PROPN
afs-72416	135	8	.	.	PROPN
afs-72416	136	1	(	(	PUNCT
afs-72416	136	2	1990	1990	NUM
afs-72416	136	3	)	)	PUNCT
afs-72416	136	4	that	that	SCONJ
afs-72416	136	5	agnq	agnq	NOUN
afs-72416	136	6	3	3	NUM
afs-72416	136	7	has	have	VERB
afs-72416	136	8	the	the	DET
afs-72416	136	9	most	most	ADV
afs-72416	136	10	favourable	favourable	ADJ
afs-72416	136	11	effect	effect	NOUN
afs-72416	136	12	on	on	ADP
afs-72416	136	13	plant	plant	NOUN
afs-72416	136	14	regeneration	regeneration	NOUN
afs-72416	136	15	when	when	SCONJ
afs-72416	136	16	applied	apply	VERB
afs-72416	136	17	at	at	ADP
afs-72416	136	18	a	a	DET
afs-72416	136	19	later	later	ADJ
afs-72416	136	20	stage	stage	NOUN
afs-72416	136	21	of	of	ADP
afs-72416	136	22	callus	callus	ADJ
afs-72416	136	23	development	development	NOUN
afs-72416	136	24	(	(	PUNCT
afs-72416	136	25	when	when	SCONJ
afs-72416	136	26	morphogene	morphogene	ADJ
afs-72416	136	27	zones	zone	NOUN
afs-72416	136	28	had	have	AUX
afs-72416	136	29	already	already	ADV
afs-72416	136	30	been	be	AUX
afs-72416	136	31	formed	form	VERB
afs-72416	136	32	)	)	PUNCT
afs-72416	136	33	rather	rather	ADV
afs-72416	136	34	than	than	ADP
afs-72416	136	35	right	right	ADV
afs-72416	136	36	from	from	ADP
afs-72416	136	37	thebeginning	thebeginne	VERB
afs-72416	136	38	of	of	ADP
afs-72416	136	39	callus	callus	ADJ
afs-72416	136	40	induction	induction	NOUN
afs-72416	136	41	.	.	PUNCT
afs-72416	137	1	acknowledgements	acknowledgement	NOUN
afs-72416	137	2	.	.	PUNCT
afs-72416	138	1	the	the	DET
afs-72416	138	2	authors	author	NOUN
afs-72416	138	3	are	be	AUX
afs-72416	138	4	thankful	thankful	ADJ
afs-72416	138	5	to	to	PART
afs-72416	138	6	miss	miss	VERB
afs-72416	138	7	outi	outi	PROPN
afs-72416	138	8	manninen	manninen	NOUN
afs-72416	138	9	,	,	PUNCT
afs-72416	138	10	mrs	mrs	PROPN
afs-72416	138	11	.	.	PROPN
afs-72416	138	12	maija	maija	PROPN
afs-72416	138	13	-	-	PUNCT
afs-72416	138	14	liisa	liisa	NOUN
afs-72416	138	15	penttila	penttila	NOUN
afs-72416	138	16	,	,	PUNCT
afs-72416	138	17	mrs	mrs	PROPN
afs-72416	138	18	.	.	PROPN
afs-72416	138	19	lldiko	lldiko	PROPN
afs-72416	138	20	d.	d.	PROPN
afs-72416	138	21	bartok	bartok	PROPN
afs-72416	138	22	,	,	PUNCT
afs-72416	138	23	for	for	ADP
afs-72416	138	24	their	their	PRON
afs-72416	138	25	skilful	skilful	ADJ
afs-72416	138	26	assistance	assistance	NOUN
afs-72416	138	27	and	and	CCONJ
afs-72416	138	28	mr	mr	PROPN
afs-72416	138	29	.	.	PROPN
afs-72416	138	30	b.	b.	PROPN
afs-72416	138	31	dusha	dusha	PROPN
afs-72416	138	32	and	and	CCONJ
afs-72416	138	33	mr	mr	PROPN
afs-72416	138	34	.	.	PROPN
afs-72416	138	35	b.	b.	PROPN
afs-72416	138	36	kdrmdn	kdrmdn	PROPN
afs-72416	138	37	in	in	ADP
afs-72416	138	38	preparing	prepare	VERB
afs-72416	138	39	the	the	DET
afs-72416	138	40	photographic	photographic	ADJ
afs-72416	138	41	material	material	NOUN
afs-72416	138	42	.	.	PUNCT
afs-72416	139	1	they	they	PRON
afs-72416	139	2	also	also	ADV
afs-72416	139	3	wish	wish	VERB
afs-72416	139	4	to	to	PART
afs-72416	139	5	thank	thank	VERB
afs-72416	139	6	mr	mr	PROPN
afs-72416	139	7	.	.	PROPN
afs-72416	139	8	l.	l.	PROPN
afs-72416	139	9	nagy	nagy	PROPN
afs-72416	139	10	for	for	ADP
afs-72416	139	11	kindly	kindly	ADV
afs-72416	139	12	supplying	supply	VERB
afs-72416	139	13	one	one	NUM
afs-72416	139	14	part	part	NOUN
afs-72416	139	15	of	of	ADP
afs-72416	139	16	seed	seed	NOUN
afs-72416	139	17	material	material	NOUN
afs-72416	139	18	.	.	PUNCT
afs-72416	140	1	this	this	DET
afs-72416	140	2	work	work	NOUN
afs-72416	140	3	was	be	AUX
afs-72416	140	4	supported	support	VERB
afs-72416	140	5	by	by	ADP
afs-72416	140	6	j.	j.	PROPN
afs-72416	140	7	pauk	pauk	PROPN
afs-72416	140	8	’s	’s	PART
afs-72416	140	9	6	6	NUM
afs-72416	140	10	-	-	PUNCT
afs-72416	140	11	month	month	NOUN
afs-72416	140	12	fellowship	fellowship	NOUN
afs-72416	140	13	in	in	ADP
afs-72416	140	14	finland	finland	PROPN
afs-72416	140	15	.	.	PUNCT
afs-72416	141	1	references	reference	NOUN
afs-72416	141	2	sidney	sidney	PROPN
afs-72416	141	3	,	,	PUNCT
afs-72416	141	4	d.l	d.l	PROPN
afs-72416	141	5	.	.	PROPN
afs-72416	141	6	,	,	PUNCT
afs-72416	141	7	shepard	shepard	NOUN
afs-72416	141	8	,	,	PUNCT
afs-72416	141	9	j.f	j.f	PROPN
afs-72416	141	10	.	.	PROPN
afs-72416	141	11	,	,	PUNCT
afs-72416	141	12	kaleikau	kaleikau	PROPN
afs-72416	141	13	,	,	PUNCT
afs-72416	141	14	e.	e.	PROPN
afs-72416	141	15	1983	1983	NUM
afs-72416	141	16	.	.	PUNCT
afs-72416	142	1	regeneration	regeneration	NOUN
afs-72416	142	2	of	of	ADP
afs-72416	142	3	plants	plant	NOUN
afs-72416	142	4	from	from	ADP
afs-72416	142	5	mesophylle	mesophylle	ADJ
afs-72416	142	6	protoplasts	protoplast	NOUN
afs-72416	142	7	of	of	ADP
afs-72416	142	8	brassica	brassica	PROPN
afs-72416	142	9	oleracea	oleracea	NOUN
afs-72416	142	10	.	.	PUNCT
afs-72416	143	1	protoplasma	protoplasma	PROPN
afs-72416	143	2	117	117	NUM
afs-72416	143	3	:	:	PUNCT
afs-72416	143	4	89—92	89—92	NUM
afs-72416	143	5	.	.	PUNCT
afs-72416	143	6	eapen	eapen	PROPN
afs-72416	143	7	,	,	PUNCT
afs-72416	143	8	s.	s.	PROPN
afs-72416	143	9	,	,	PUNCT
afs-72416	143	10	abraham	abraham	PROPN
afs-72416	143	11	,	,	PUNCT
afs-72416	143	12	v.	v.	PROPN
afs-72416	143	13	,	,	PUNCT
afs-72416	143	14	gerdemann	gerdemann	NOUN
afs-72416	143	15	,	,	PUNCT
afs-72416	143	16	m.	m.	NOUN
afs-72416	143	17	,	,	PUNCT
afs-72416	143	18	schieder	schieder	NOUN
afs-72416	143	19	,	,	PUNCT
afs-72416	143	20	o.	o.	NOUN
afs-72416	143	21	1989	1989	NUM
afs-72416	143	22	.	.	PUNCT
afs-72416	144	1	direct	direct	ADJ
afs-72416	144	2	somatic	somatic	ADJ
afs-72416	144	3	embryogenesis	embryogenesis	NOUN
afs-72416	144	4	,	,	PUNCT
afs-72416	144	5	plant	plant	NOUN
afs-72416	144	6	regeneration	regeneration	NOUN
afs-72416	144	7	and	and	CCONJ
afs-72416	144	8	evaluation	evaluation	NOUN
afs-72416	144	9	of	of	ADP
afs-72416	144	10	plants	plant	NOUN
afs-72416	144	11	obtained	obtain	VERB
afs-72416	144	12	from	from	ADP
afs-72416	144	13	mesophyll	mesophyll	NOUN
afs-72416	144	14	protoplasts	protoplast	NOUN
afs-72416	144	15	of	of	ADP
afs-72416	144	16	brassica	brassica	PROPN
afs-72416	144	17	juncea	juncea	PROPN
afs-72416	144	18	.	.	PUNCT
afs-72416	145	1	annals	annal	NOUN
afs-72416	145	2	of	of	ADP
afs-72416	145	3	botany	botany	NOUN
afs-72416	145	4	63	63	NUM
afs-72416	145	5	,	,	PUNCT
afs-72416	145	6	369—372	369—372	NUM
afs-72416	145	7	.	.	PUNCT
afs-72416	145	8	glimelius	glimelius	PROPN
afs-72416	145	9	,	,	PUNCT
afs-72416	145	10	k.	k.	PROPN
afs-72416	145	11	1984	1984	NUM
afs-72416	145	12	.	.	PUNCT
afs-72416	146	1	high	high	ADJ
afs-72416	146	2	growth	growth	NOUN
afs-72416	146	3	rate	rate	NOUN
afs-72416	146	4	and	and	CCONJ
afs-72416	146	5	regeneration	regeneration	NOUN
afs-72416	146	6	capacity	capacity	NOUN
afs-72416	146	7	ofhypocotyl	ofhypocotyl	NOUN
afs-72416	146	8	protoplast	protoplast	NOUN
afs-72416	146	9	in	in	ADP
afs-72416	146	10	some	some	DET
afs-72416	146	11	brassicaceae	brassicaceae	NOUN
afs-72416	146	12	.	.	PUNCT
afs-72416	147	1	physiol	physiol	PROPN
afs-72416	147	2	.	.	PUNCT
afs-72416	148	1	plant	plant	NOUN
afs-72416	148	2	.	.	PUNCT
afs-72416	149	1	61	61	NUM
afs-72416	149	2	:	:	SYM
afs-72416	149	3	38—44	38—44	NUM
afs-72416	149	4	.	.	PUNCT
afs-72416	150	1	jourdan	jourdan	PROPN
afs-72416	150	2	,	,	PUNCT
afs-72416	150	3	p.s	p.s	PROPN
afs-72416	150	4	.	.	PROPN
afs-72416	150	5	earle	earle	PROPN
afs-72416	150	6	,	,	PUNCT
afs-72416	150	7	e.-d	e.-d	NOUN
afs-72416	150	8	.	.	PUNCT
afs-72416	150	9	1989	1989	NUM
afs-72416	150	10	.	.	PUNCT
afs-72416	151	1	genotypic	genotypic	ADJ
afs-72416	151	2	variability	variability	NOUN
afs-72416	151	3	in	in	ADP
afs-72416	151	4	the	the	DET
afs-72416	151	5	frequency	frequency	NOUN
afs-72416	151	6	of	of	ADP
afs-72416	151	7	plant	plant	NOUN
afs-72416	151	8	regeneration	regeneration	NOUN
afs-72416	151	9	from	from	ADP
afs-72416	151	10	leaf	leaf	NOUN
afs-72416	151	11	protoplasts	protoplast	NOUN
afs-72416	151	12	of	of	ADP
afs-72416	151	13	four	four	NUM
afs-72416	151	14	brassica	brassica	PROPN
afs-72416	151	15	spp	spp	NOUN
afs-72416	151	16	.	.	PUNCT
afs-72416	152	1	and	and	CCONJ
afs-72416	152	2	of	of	ADP
afs-72416	152	3	raphanus	raphanus	PROPN
afs-72416	152	4	salivas	salivas	PROPN
afs-72416	152	5	.	.	PUNCT
afs-72416	153	1	j.	j.	PROPN
afs-72416	153	2	amer	amer	PROPN
afs-72416	153	3	.	.	PUNCT
afs-72416	153	4	soc	soc	PROPN
afs-72416	153	5	.	.	PUNCT
afs-72416	154	1	hort	hort	PROPN
afs-72416	154	2	.	.	PUNCT
afs-72416	155	1	sci	sci	PROPN
afs-72416	155	2	.	.	PROPN
afs-72416	156	1	114	114	NUM
afs-72416	157	1	(	(	PUNCT
afs-72416	157	2	2	2	NUM
afs-72416	157	3	):	):	PUNCT
afs-72416	157	4	343—349	343—349	PROPN
afs-72416	157	5	.	.	PUNCT
afs-72416	158	1	kao	kao	PROPN
afs-72416	158	2	,	,	PUNCT
afs-72416	158	3	k.n	k.n	PROPN
afs-72416	158	4	.	.	PROPN
afs-72416	158	5	michayluk	michayluk	PROPN
afs-72416	158	6	,	,	PUNCT
afs-72416	158	7	m.r	m.r	PROPN
afs-72416	158	8	.	.	PROPN
afs-72416	158	9	1975	1975	NUM
afs-72416	158	10	.	.	PUNCT
afs-72416	159	1	nutritional	nutritional	ADJ
afs-72416	159	2	requirements	requirement	NOUN
afs-72416	159	3	for	for	ADP
afs-72416	159	4	growth	growth	NOUN
afs-72416	159	5	of	of	ADP
afs-72416	159	6	vida	vida	PROPN
afs-72416	159	7	hajaslana	hajaslana	PROPN
afs-72416	159	8	cells	cell	NOUN
afs-72416	159	9	and	and	CCONJ
afs-72416	159	10	protoplasts	protoplast	NOUN
afs-72416	159	11	at	at	ADP
afs-72416	159	12	very	very	ADV
afs-72416	159	13	low	low	ADJ
afs-72416	159	14	population	population	NOUN
afs-72416	159	15	density	density	NOUN
afs-72416	159	16	in	in	ADP
afs-72416	159	17	liquid	liquid	ADJ
afs-72416	159	18	media	medium	NOUN
afs-72416	159	19	.	.	PUNCT
afs-72416	160	1	planta	planta	NOUN
afs-72416	160	2	126	126	NUM
afs-72416	160	3	:	:	PUNCT
afs-72416	160	4	105—110	105—110	PROPN
afs-72416	160	5	.	.	PUNCT
afs-72416	161	1	kartha	kartha	PROPN
afs-72416	161	2	,	,	PUNCT
afs-72416	161	3	k.k	k.k	PROPN
afs-72416	161	4	.	.	PROPN
afs-72416	161	5	michayluk	michayluk	PROPN
afs-72416	161	6	,	,	PUNCT
afs-72416	161	7	m.r	m.r	PROPN
afs-72416	161	8	.	.	PROPN
afs-72416	161	9	kao	kao	PROPN
afs-72416	161	10	,	,	PUNCT
afs-72416	161	11	k.n	k.n	PROPN
afs-72416	161	12	.	.	PROPN
afs-72416	161	13	gamborg	gamborg	PROPN
afs-72416	161	14	,	,	PUNCT
afs-72416	161	15	o.l	o.l	PROPN
afs-72416	161	16	.	.	PROPN
afs-72416	161	17	constable	constable	NOUN
afs-72416	161	18	,	,	PUNCT
afs-72416	161	19	f.	f.	PROPN
afs-72416	161	20	1974	1974	NUM
afs-72416	161	21	.	.	PUNCT
afs-72416	162	1	callus	callus	ADJ
afs-72416	162	2	formation	formation	NOUN
afs-72416	162	3	and	and	CCONJ
afs-72416	162	4	plant	plant	NOUN
afs-72416	162	5	regeneration	regeneration	NOUN
afs-72416	162	6	from	from	ADP
afs-72416	162	7	mesophyll	mesophyll	NOUN
afs-72416	162	8	protoplast	protoplast	NOUN
afs-72416	162	9	of	of	ADP
afs-72416	162	10	rape	rape	NOUN
afs-72416	162	11	plants	plant	NOUN
afs-72416	162	12	(	(	PUNCT
afs-72416	162	13	brassica	brassica	PROPN
afs-72416	162	14	napuscv	napuscv	PROPN
afs-72416	162	15	.	.	PUNCT
afs-72416	163	1	zephir	zephir	NOUN
afs-72416	163	2	)	)	PUNCT
afs-72416	163	3	plant	plant	NOUN
afs-72416	163	4	sci	sci	PROPN
afs-72416	163	5	.	.	PUNCT
afs-72416	163	6	lett	lett	PROPN
afs-72416	163	7	.	.	PUNCT
afs-72416	164	1	3	3	NUM
afs-72416	164	2	:	:	PUNCT
afs-72416	164	3	265—271	265—271	NOUN
afs-72416	164	4	.	.	PUNCT
afs-72416	165	1	kohlenbach	kohlenbach	PROPN
afs-72416	165	2	,	,	PUNCT
afs-72416	165	3	h.w	h.w	PROPN
afs-72416	165	4	.	.	PROPN
afs-72416	165	5	,	,	PUNCT
afs-72416	165	6	wenzel	wenzel	PROPN
afs-72416	165	7	,	,	PUNCT
afs-72416	165	8	g.	g.	PROPN
afs-72416	165	9	,	,	PUNCT
afs-72416	165	10	hoffmann	hoffmann	PROPN
afs-72416	165	11	,	,	PUNCT
afs-72416	165	12	f.	f.	PROPN
afs-72416	165	13	1982	1982	NUM
afs-72416	165	14	.	.	PUNCT
afs-72416	166	1	regeneration	regeneration	NOUN
afs-72416	166	2	of	of	ADP
afs-72416	166	3	brassica	brassica	NOUN
afs-72416	166	4	napus	napus	PROPN
afs-72416	166	5	plantlets	plantlet	NOUN
afs-72416	166	6	in	in	ADP
afs-72416	166	7	cultures	culture	NOUN
afs-72416	166	8	from	from	ADP
afs-72416	166	9	isolated	isolated	ADJ
afs-72416	166	10	protoplasts	protoplast	NOUN
afs-72416	166	11	of	of	ADP
afs-72416	166	12	haploid	haploid	NOUN
afs-72416	166	13	stem	stem	NOUN
afs-72416	166	14	embryos	embryo	NOUN
afs-72416	166	15	as	as	ADP
afs-72416	166	16	compared	compare	VERB
afs-72416	166	17	with	with	ADP
afs-72416	166	18	leaf	leaf	NOUN
afs-72416	166	19	protoplasts	protoplast	NOUN
afs-72416	166	20	.	.	PUNCT
afs-72416	167	1	z.	z.	PROPN
afs-72416	167	2	pflanzenphysiol	pflanzenphysiol	PROPN
afs-72416	167	3	.	.	PUNCT
afs-72416	168	1	bd	bd	PROPN
afs-72416	168	2	.	.	PROPN
afs-72416	168	3	105	105	NUM
afs-72416	168	4	.	.	PUNCT
afs-72416	169	1	s.	s.	PROPN
afs-72416	169	2	131—142	131—142	PROPN
afs-72416	169	3	.	.	PUNCT
afs-72416	170	1	lentini	lentini	PROPN
afs-72416	170	2	,	,	PUNCT
afs-72416	170	3	z.	z.	PROPN
afs-72416	170	4	mursell	mursell	PROPN
afs-72416	170	5	,	,	PUNCT
afs-72416	170	6	m.a	m.a	PROPN
afs-72416	170	7	.	.	PROPN
afs-72416	170	8	mutschler	mutschler	NOUN
afs-72416	170	9	,	,	PUNCT
afs-72416	170	10	m.a	m.a	PROPN
afs-72416	170	11	.	.	PROPN
afs-72416	170	12	earle	earle	PROPN
afs-72416	170	13	,	,	PUNCT
afs-72416	170	14	e.d	e.d	PROPN
afs-72416	170	15	.	.	PROPN
afs-72416	170	16	1988	1988	NUM
afs-72416	170	17	.	.	PUNCT
afs-72416	171	1	ethylen	ethylen	NOUN
afs-72416	171	2	generation	generation	NOUN
afs-72416	171	3	and	and	CCONJ
afs-72416	171	4	reversal	reversal	NOUN
afs-72416	171	5	of	of	ADP
afs-72416	171	6	ethylene	ethylene	NOUN
afs-72416	171	7	effects	effect	NOUN
afs-72416	171	8	during	during	ADP
afs-72416	171	9	development	development	NOUN
afs-72416	171	10	in	in	ADP
afs-72416	171	11	vitro	vitro	X
afs-72416	171	12	of	of	ADP
afs-72416	171	13	rapidcycling	rapidcycle	VERB
afs-72416	171	14	brassica	brassica	PROPN
afs-72416	171	15	campestris	campestris	PROPN
afs-72416	171	16	l.	l.	PROPN
afs-72416	171	17	plant	plant	PROPN
afs-72416	171	18	sci	sci	PROPN
afs-72416	171	19	.	.	PROPN
afs-72416	172	1	54	54	NUM
afs-72416	172	2	:	:	PUNCT
afs-72416	172	3	75—81	75—81	NUM
afs-72416	172	4	.	.	PUNCT
afs-72416	173	1	li	li	PROPN
afs-72416	173	2	,	,	PUNCT
afs-72416	173	3	l.c	l.c	PROPN
afs-72416	173	4	.	.	PROPN
afs-72416	173	5	kohlenbach	kohlenbach	PROPN
afs-72416	173	6	,	,	PUNCT
afs-72416	173	7	h.w	h.w	PROPN
afs-72416	173	8	.	.	PROPN
afs-72416	173	9	1982	1982	NUM
afs-72416	173	10	.	.	PUNCT
afs-72416	174	1	somatic	somatic	ADJ
afs-72416	174	2	embryogenesis	embryogenesis	NOUN
afs-72416	174	3	in	in	ADP
afs-72416	174	4	quite	quite	DET
afs-72416	174	5	a	a	DET
afs-72416	174	6	direct	direct	ADJ
afs-72416	174	7	way	way	NOUN
afs-72416	174	8	in	in	ADP
afs-72416	174	9	cultures	culture	NOUN
afs-72416	174	10	of	of	ADP
afs-72416	174	11	mesophyll	mesophyll	NOUN
afs-72416	174	12	protoplasts	protoplast	NOUN
afs-72416	174	13	of	of	ADP
afs-72416	174	14	brassica	brassica	PROPN
afs-72416	174	15	napus	napus	PROPN
afs-72416	174	16	l.	l.	PROPN
afs-72416	174	17	plant	plant	PROPN
afs-72416	174	18	cell	cell	NOUN
afs-72416	174	19	rep	rep	PROPN
afs-72416	174	20	.	.	PROPN
afs-72416	174	21	1	1	NUM
afs-72416	174	22	:	:	PUNCT
afs-72416	174	23	209—211	209—211	NOUN
afs-72416	174	24	.	.	PUNCT
afs-72416	175	1	loudon	loudon	PROPN
afs-72416	175	2	,	,	PUNCT
afs-72416	175	3	p.t	p.t	PROPN
afs-72416	175	4	.	.	PROPN
afs-72416	175	5	nelson	nelson	PROPN
afs-72416	175	6	,	,	PUNCT
afs-72416	175	7	r.s	r.s	PROPN
afs-72416	175	8	.	.	PROPN
afs-72416	175	9	ingram	ingram	PROPN
afs-72416	175	10	,	,	PUNCT
afs-72416	175	11	d.s	d.s	PROPN
afs-72416	175	12	.	.	PROPN
afs-72416	175	13	1989	1989	NUM
afs-72416	175	14	.	.	PUNCT
afs-72416	176	1	studies	study	NOUN
afs-72416	176	2	of	of	ADP
afs-72416	176	3	protoplast	protoplast	NOUN
afs-72416	176	4	culture	culture	NOUN
afs-72416	176	5	and	and	CCONJ
afs-72416	176	6	plant	plant	NOUN
afs-72416	176	7	regeneration	regeneration	NOUN
afs-72416	176	8	from	from	ADP
afs-72416	176	9	commercial	commercial	ADJ
afs-72416	176	10	and	and	CCONJ
afs-72416	176	11	rapid	rapid	ADJ
afs-72416	176	12	-	-	PUNCT
afs-72416	176	13	cycling	cycling	NOUN
afs-72416	176	14	brassica	brassica	NOUN
afs-72416	176	15	species	specie	NOUN
afs-72416	176	16	.	.	PUNCT
afs-72416	177	1	plant	plant	NOUN
afs-72416	177	2	cell	cell	NOUN
afs-72416	177	3	tiss	tis	VERB
afs-72416	177	4	.	.	PUNCT
afs-72416	178	1	org	org	PROPN
afs-72416	178	2	.	.	PUNCT
afs-72416	179	1	cult	cult	PROPN
afs-72416	179	2	.	.	PUNCT
afs-72416	180	1	19	19	NUM
afs-72416	180	2	:	:	PUNCT
afs-72416	180	3	213—224	213—224	NUM
afs-72416	180	4	.	.	PUNCT
afs-72416	181	1	murashige	murashige	PROPN
afs-72416	181	2	,	,	PUNCT
afs-72416	181	3	t.	t.	PROPN
afs-72416	181	4	skoog	skoog	PROPN
afs-72416	181	5	,	,	PUNCT
afs-72416	181	6	f.	f.	PROPN
afs-72416	181	7	1962	1962	NUM
afs-72416	181	8	.	.	PUNCT
afs-72416	182	1	a	a	DET
afs-72416	182	2	revised	revise	VERB
afs-72416	182	3	medium	medium	NOUN
afs-72416	182	4	for	for	ADP
afs-72416	182	5	rapid	rapid	ADJ
afs-72416	182	6	growth	growth	NOUN
afs-72416	182	7	and	and	CCONJ
afs-72416	182	8	bioassays	bioassay	NOUN
afs-72416	182	9	with	with	ADP
afs-72416	182	10	tobacco	tobacco	NOUN
afs-72416	182	11	tissue	tissue	NOUN
afs-72416	182	12	cultures	culture	NOUN
afs-72416	182	13	.	.	PUNCT
afs-72416	183	1	physiol	physiol	PROPN
afs-72416	183	2	.	.	PUNCT
afs-72416	184	1	plantarum	plantarum	VERB
afs-72416	184	2	15	15	NUM
afs-72416	184	3	:	:	PUNCT
afs-72416	185	1	473—497	473—497	NOUN
afs-72416	185	2	.	.	PUNCT
afs-72416	186	1	nagy	nagy	PROPN
afs-72416	186	2	,	,	PUNCT
afs-72416	186	3	j.	j.	PROPN
afs-72416	186	4	maliga	maliga	PROPN
afs-72416	186	5	,	,	PUNCT
afs-72416	186	6	p.	p.	NOUN
afs-72416	186	7	1976	1976	NUM
afs-72416	186	8	.	.	PUNCT
afs-72416	187	1	callus	callus	ADJ
afs-72416	187	2	induction	induction	NOUN
afs-72416	187	3	and	and	CCONJ
afs-72416	187	4	plant	plant	NOUN
afs-72416	187	5	regeneration	regeneration	NOUN
afs-72416	187	6	from	from	ADP
afs-72416	187	7	mesophyll	mesophyll	NOUN
afs-72416	187	8	protoplasts	protoplast	NOUN
afs-72416	187	9	of	of	ADP
afs-72416	187	10	nicotiana	nicotiana	PROPN
afs-72416	187	11	silveslris	silveslris	PROPN
afs-72416	187	12	.	.	PUNCT
afs-72416	188	1	z.	z.	PROPN
afs-72416	188	2	pflanzenphysiol	pflanzenphysiol	PROPN
afs-72416	188	3	.	.	PUNCT
afs-72416	189	1	78	78	NUM
afs-72416	189	2	:	:	PUNCT
afs-72416	189	3	453—455	453—455	NUM
afs-72416	189	4	.	.	PUNCT
afs-72416	189	5	purnhauser	purnhauser	PROPN
afs-72416	189	6	,	,	PUNCT
afs-72416	189	7	l.	l.	PROPN
afs-72416	189	8	medoyesi	medoyesi	PROPN
afs-72416	189	9	,	,	PUNCT
afs-72416	189	10	l.	l.	PROPN
afs-72416	189	11	czak6	czak6	PROPN
afs-72416	189	12	,	,	PUNCT
afs-72416	189	13	m.	m.	PROPN
afs-72416	189	14	dix	dix	PROPN
afs-72416	189	15	,	,	PUNCT
afs-72416	189	16	377	377	NUM
afs-72416	189	17	p.j	p.j	PROPN
afs-72416	189	18	.	.	PROPN
afs-72416	189	19	marton	marton	PROPN
afs-72416	189	20	,	,	PUNCT
afs-72416	189	21	l.	l.	PROPN
afs-72416	189	22	1987	1987	NUM
afs-72416	189	23	.	.	PUNCT
afs-72416	190	1	stimulation	stimulation	NOUN
afs-72416	190	2	of	of	ADP
afs-72416	190	3	shoot	shoot	NOUN
afs-72416	190	4	regeneration	regeneration	NOUN
afs-72416	190	5	in	in	ADP
afs-72416	190	6	triticum	triticum	NOUN
afs-72416	190	7	aeslivum	aeslivum	NOUN
afs-72416	190	8	and	and	CCONJ
afs-72416	190	9	nicotiana	nicotiana	PROPN
afs-72416	190	10	plumbaginifolia	plumbaginifolia	PROPN
afs-72416	190	11	viv	viv	PROPN
afs-72416	190	12	.	.	PUNCT
afs-72416	191	1	tissue	tissue	NOUN
afs-72416	191	2	culture	culture	NOUN
afs-72416	191	3	using	use	VERB
afs-72416	191	4	the	the	DET
afs-72416	191	5	ethylen	ethylen	NOUN
afs-72416	191	6	inhibitor	inhibitor	NOUN
afs-72416	191	7	agng3	agng3	PROPN
afs-72416	191	8	plant	plant	NOUN
afs-72416	191	9	cell	cell	NOUN
afs-72416	191	10	rep	rep	PROPN
afs-72416	191	11	.	.	PROPN
afs-72416	191	12	6	6	NUM
afs-72416	191	13	:	:	PUNCT
afs-72416	191	14	i—4	i—4	PROPN
afs-72416	191	15	.	.	PUNCT
afs-72416	192	1	robertson	robertson	PROPN
afs-72416	192	2	,	,	PUNCT
afs-72416	192	3	d.	d.	PROPN
afs-72416	192	4	earle	earle	PROPN
afs-72416	192	5	,	,	PUNCT
afs-72416	192	6	e.d	e.d	PROPN
afs-72416	192	7	.	.	PROPN
afs-72416	192	8	mutschler	mutschler	PROPN
afs-72416	192	9	,	,	PUNCT
afs-72416	192	10	m.a	m.a	PROPN
afs-72416	192	11	.	.	PROPN
afs-72416	192	12	1984	1984	NUM
afs-72416	192	13	.	.	PUNCT
afs-72416	193	1	enhanced	enhance	VERB
afs-72416	193	2	rates	rate	NOUN
afs-72416	193	3	ofplant	ofplant	VERB
afs-72416	193	4	regeneration	regeneration	NOUN
afs-72416	193	5	from	from	ADP
afs-72416	193	6	protoplasts	protoplast	NOUN
afs-72416	193	7	of	of	ADP
afs-72416	193	8	cultivar	cultivar	NOUN
afs-72416	193	9	green	green	ADJ
afs-72416	193	10	comet	comet	NOUN
afs-72416	193	11	broccoli	broccoli	PROPN
afs-72416	193	12	brassica	brassica	PROPN
afs-72416	193	13	oleracea	oleracea	NOUN
afs-72416	193	14	.	.	PUNCT
afs-72416	194	1	plant	plant	NOUN
afs-72416	194	2	physiol	physiol	NOUN
afs-72416	194	3	.	.	PUNCT
afs-72416	195	1	75	75	NUM
afs-72416	195	2	:	:	SYM
afs-72416	195	3	134	134	NUM
afs-72416	195	4	.	.	PUNCT
afs-72416	196	1	schenk	schenk	PROPN
afs-72416	196	2	,	,	PUNCT
afs-72416	196	3	h.r	h.r	PROPN
afs-72416	196	4	.	.	PROPN
afs-72416	196	5	,	,	PUNCT
afs-72416	196	6	hoffmann	hoffmann	PROPN
afs-72416	196	7	,	,	PUNCT
afs-72416	196	8	f.	f.	PROPN
afs-72416	196	9	1979	1979	NUM
afs-72416	196	10	.	.	PUNCT
afs-72416	197	1	callus	callus	ADJ
afs-72416	197	2	and	and	CCONJ
afs-72416	197	3	root	root	NOUN
afs-72416	197	4	regeneration	regeneration	NOUN
afs-72416	197	5	from	from	ADP
afs-72416	197	6	mesophyll	mesophyll	NOUN
afs-72416	197	7	protoplasts	protoplast	NOUN
afs-72416	197	8	of	of	ADP
afs-72416	197	9	basic	basic	ADJ
afs-72416	197	10	brassica	brassica	NOUN
afs-72416	197	11	species	specie	NOUN
afs-72416	197	12	:	:	PUNCT
afs-72416	197	13	b.	b.	PROPN
afs-72416	197	14	campestris	campestris	PROPN
afs-72416	197	15	,	,	PUNCT
afs-72416	197	16	b.	b.	PROPN
afs-72416	197	17	oleracea	oleracea	PROPN
afs-72416	197	18	and	and	CCONJ
afs-72416	197	19	b.	b.	PROPN
afs-72416	197	20	nigrra	nigrra	PROPN
afs-72416	197	21	.	.	PUNCT
afs-72416	198	1	z.	z.	PROPN
afs-72416	198	2	pflanzenziichtg	pflanzenziichtg	PROPN
afs-72416	198	3	.	.	PUNCT
afs-72416	199	1	82	82	NUM
afs-72416	199	2	:	:	PUNCT
afs-72416	199	3	354—360	354—360	PROPN
afs-72416	199	4	.	.	PUNCT
afs-72416	200	1	shillito	shillito	PROPN
afs-72416	200	2	,	,	PUNCT
afs-72416	200	3	r.d	r.d	PROPN
afs-72416	200	4	.	.	PROPN
afs-72416	200	5	,	,	PUNCT
afs-72416	200	6	paszkowski	paszkowski	PROPN
afs-72416	200	7	,	,	PUNCT
afs-72416	200	8	j.	j.	PROPN
afs-72416	200	9	,	,	PUNCT
afs-72416	200	10	potrikus	potriku	NOUN
afs-72416	200	11	,	,	PUNCT
afs-72416	200	12	i.	i.	PROPN
afs-72416	200	13	1983	1983	NUM
afs-72416	200	14	.	.	PUNCT
afs-72416	201	1	agarose	agarose	NOUN
afs-72416	201	2	plating	plating	NOUN
afs-72416	201	3	and	and	CCONJ
afs-72416	201	4	bead	bead	NOUN
afs-72416	201	5	type	type	NOUN
afs-72416	201	6	technique	technique	NOUN
afs-72416	201	7	enable	enable	ADJ
afs-72416	201	8	and	and	CCONJ
afs-72416	201	9	stimulate	stimulate	VERB
afs-72416	201	10	development	development	NOUN
afs-72416	201	11	of	of	ADP
afs-72416	201	12	protoplast	protoplast	NOUN
afs-72416	201	13	-	-	PUNCT
afs-72416	201	14	derived	derive	VERB
afs-72416	201	15	colonies	colony	NOUN
afs-72416	201	16	in	in	ADP
afs-72416	201	17	a	a	DET
afs-72416	201	18	number	number	NOUN
afs-72416	201	19	of	of	ADP
afs-72416	201	20	plant	plant	NOUN
afs-72416	201	21	.	.	PUNCT
afs-72416	202	1	songstad	songstad	NOUN
afs-72416	202	2	,	,	PUNCT
afs-72416	202	3	d.d	d.d	PROPN
afs-72416	202	4	.	.	PROPN
afs-72416	202	5	duncan	duncan	PROPN
afs-72416	202	6	,	,	PUNCT
afs-72416	202	7	d.r	d.r	PROPN
afs-72416	202	8	.	.	PROPN
afs-72416	202	9	widholm	widholm	PROPN
afs-72416	202	10	,	,	PUNCT
afs-72416	202	11	j.	j.	PROPN
afs-72416	202	12	m.	m.	PROPN
afs-72416	202	13	1988	1988	NUM
afs-72416	202	14	.	.	PUNCT
afs-72416	203	1	effect	effect	NOUN
afs-72416	203	2	of	of	ADP
afs-72416	203	3	1	1	NUM
afs-72416	203	4	-	-	PUNCT
afs-72416	203	5	aminocyclopropane	aminocyclopropane	ADJ
afs-72416	203	6	-	-	PUNCT
afs-72416	203	7	l	l	NOUN
afs-72416	203	8	-	-	ADJ
afs-72416	203	9	carboxylic	carboxylic	ADJ
afs-72416	203	10	acid	acid	NOUN
afs-72416	203	11	,	,	PUNCT
afs-72416	203	12	silver	silver	NOUN
afs-72416	203	13	nitrate	nitrate	NOUN
afs-72416	203	14	and	and	CCONJ
afs-72416	203	15	norbornadiene	norbornadiene	NOUN
afs-72416	203	16	on	on	ADP
afs-72416	203	17	plant	plant	NOUN
afs-72416	203	18	regeneration	regeneration	NOUN
afs-72416	203	19	.	.	PUNCT
afs-72416	204	1	plant	plant	NOUN
afs-72416	204	2	cell	cell	NOUN
afs-72416	204	3	rep	rep	NOUN
afs-72416	204	4	.	.	PROPN
afs-72416	204	5	7	7	NUM
afs-72416	204	6	:	:	PUNCT
afs-72416	204	7	262—265	262—265	NOUN
afs-72416	204	8	.	.	PUNCT
afs-72416	205	1	ulrich	ulrich	PROPN
afs-72416	205	2	,	,	PUNCT
afs-72416	205	3	t.h	t.h	PROPN
afs-72416	205	4	.	.	PROPN
afs-72416	205	5	chowdhury	chowdhury	PROPN
afs-72416	205	6	,	,	PUNCT
afs-72416	206	1	j.b	j.b	PROPN
afs-72416	206	2	.	.	PUNCT
afs-72416	206	3	wildholm	wildholm	PROPN
afs-72416	206	4	,	,	PUNCT
afs-72416	206	5	j.m	j.m	PROPN
afs-72416	206	6	.	.	NOUN
afs-72416	206	7	1980	1980	NUM
afs-72416	206	8	.	.	PUNCT
afs-72416	207	1	callus	callus	ADJ
afs-72416	207	2	and	and	CCONJ
afs-72416	207	3	root	root	NOUN
afs-72416	207	4	formation	formation	NOUN
afs-72416	207	5	from	from	ADP
afs-72416	207	6	mesophyll	mesophyll	NOUN
afs-72416	207	7	protoplasts	protoplast	NOUN
afs-72416	207	8	of	of	ADP
afs-72416	207	9	brassica	brassica	PROPN
afs-72416	207	10	rapa	rapa	VERB
afs-72416	207	11	.	.	PUNCT
afs-72416	208	1	plant	plant	PROPN
afs-72416	208	2	sci	sci	PROPN
afs-72416	208	3	.	.	PUNCT
afs-72416	208	4	lett	lett	PROPN
afs-72416	208	5	.	.	PROPN
afs-72416	209	1	19	19	NUM
afs-72416	209	2	;	;	PUNCT
afs-72416	209	3	347	347	NUM
afs-72416	209	4	—	—	PUNCT
afs-72416	209	5	354	354	NUM
afs-72416	209	6	.	.	X
afs-72416	210	1	vamling	vamling	PROPN
afs-72416	210	2	,	,	PUNCT
afs-72416	210	3	k.	k.	PROPN
afs-72416	210	4	,	,	PUNCT
afs-72416	210	5	glimelius	glimelius	PROPN
afs-72416	210	6	,	,	PUNCT
afs-72416	210	7	k.	k.	PROPN
afs-72416	210	8	1990	1990	NUM
afs-72416	210	9	.	.	PUNCT
afs-72416	211	1	regeneration	regeneration	NOUN
afs-72416	211	2	of	of	ADP
afs-72416	211	3	plants	plant	NOUN
afs-72416	211	4	from	from	ADP
afs-72416	211	5	protoplasts	protoplast	NOUN
afs-72416	211	6	of	of	ADP
afs-72416	211	7	oilseed	oilseed	NOUN
afs-72416	211	8	brassica	brassica	NOUN
afs-72416	211	9	crops	crop	NOUN
afs-72416	211	10	.	.	PUNCT
afs-72416	212	1	in	in	ADP
afs-72416	212	2	:	:	PUNCT
afs-72416	212	3	bajaj	bajaj	PROPN
afs-72416	212	4	(	(	PUNCT
afs-72416	212	5	ed	ed	NOUN
afs-72416	212	6	.	.	PUNCT
afs-72416	212	7	)	)	PUNCT
afs-72416	212	8	;	;	PUNCT
afs-72416	212	9	biotechnology	biotechnology	NOUN
afs-72416	212	10	in	in	ADP
afs-72416	212	11	agriculture	agriculture	NOUN
afs-72416	212	12	and	and	CCONJ
afs-72416	212	13	forestry	forestry	NOUN
afs-72416	212	14	.	.	PUNCT
afs-72416	213	1	vol	vol	NOUN
afs-72416	213	2	.	.	PUNCT
afs-72416	214	1	10	10	NUM
afs-72416	214	2	.	.	PUNCT
afs-72416	215	1	385—417	385—417	NUM
afs-72416	215	2	.	.	PUNCT
afs-72416	215	3	springer	springer	NOUN
afs-72416	215	4	-	-	PUNCT
afs-72416	215	5	verlag	verlag	PROPN
afs-72416	215	6	,	,	PUNCT
afs-72416	215	7	heidelberg	heidelberg	PROPN
afs-72416	215	8	.	.	PUNCT
afs-72416	216	1	wenzel	wenzel	PROPN
afs-72416	216	2	,	,	PUNCT
afs-72416	216	3	g.	g.	PROPN
afs-72416	216	4	1973	1973	NUM
afs-72416	216	5	.	.	PUNCT
afs-72416	217	1	isolation	isolation	NOUN
afs-72416	217	2	of	of	ADP
afs-72416	217	3	leaf	leaf	NOUN
afs-72416	217	4	protoplasts	protoplast	NOUN
afs-72416	217	5	from	from	ADP
afs-72416	217	6	haploid	haploid	NOUN
afs-72416	217	7	plants	plant	NOUN
afs-72416	217	8	of	of	ADP
afs-72416	217	9	petunia	petunia	NOUN
afs-72416	217	10	,	,	PUNCT
afs-72416	217	11	rape	rape	NOUN
afs-72416	217	12	and	and	CCONJ
afs-72416	217	13	rye	rye	NOUN
afs-72416	217	14	.	.	PUNCT
afs-72416	218	1	z.	z.	PROPN
afs-72416	218	2	pflanzenziich	pflanzenziich	PROPN
afs-72416	218	3	.	.	PUNCT
afs-72416	219	1	69	69	NUM
afs-72416	219	2	:	:	PUNCT
afs-72416	219	3	58—61	58—61	NUM
afs-72416	219	4	.	.	PUNCT
afs-72416	220	1	williams	williams	PROPN
afs-72416	220	2	,	,	PUNCT
afs-72416	220	3	j.	j.	PROPN
afs-72416	220	4	pink	pink	PROPN
afs-72416	220	5	,	,	PUNCT
afs-72416	220	6	d.a.c	d.a.c	PROPN
afs-72416	220	7	.	.	PUNCT
afs-72416	220	8	biddington	biddington	PROPN
afs-72416	220	9	,	,	PUNCT
afs-72416	220	10	n.l	n.l	PROPN
afs-72416	220	11	.	.	PROPN
afs-72416	220	12	1990	1990	NUM
afs-72416	220	13	.	.	PUNCT
afs-72416	221	1	effect	effect	NOUN
afs-72416	221	2	of	of	ADP
afs-72416	221	3	silver	silver	ADJ
afs-72416	221	4	nitrate	nitrate	NOUN
afs-72416	221	5	on	on	ADP
afs-72416	221	6	long	long	ADJ
afs-72416	221	7	-	-	PUNCT
afs-72416	221	8	term	term	NOUN
afs-72416	221	9	culture	culture	NOUN
afs-72416	221	10	and	and	CCONJ
afs-72416	221	11	regeneration	regeneration	NOUN
afs-72416	221	12	of	of	ADP
afs-72416	221	13	callus	callus	NOUN
afs-72416	221	14	from	from	ADP
afs-72416	221	15	brassica	brassica	PROPN
afs-72416	221	16	oleracea	oleracea	PROPN
afs-72416	221	17	var	var	PROPN
afs-72416	221	18	.	.	PUNCT
afs-72416	222	1	gemmifera	gemmifera	NOUN
afs-72416	222	2	.	.	PUNCT
afs-72416	223	1	plant	plant	NOUN
afs-72416	223	2	cell	cell	NOUN
afs-72416	223	3	tissue	tissue	NOUN
afs-72416	223	4	org	org	NOUN
afs-72416	223	5	.	.	PUNCT
afs-72416	224	1	cult	cult	NOUN
afs-72416	224	2	.	.	PUNCT
afs-72416	225	1	21	21	NUM
afs-72416	225	2	:	:	PUNCT
afs-72416	225	3	61—66	61—66	X
afs-72416	225	4	.	.	PUNCT
afs-72416	226	1	xu	xu	INTJ
afs-72416	226	2	,	,	PUNCT
afs-72416	226	3	z.h	z.h	PROPN
afs-72416	226	4	.	.	PROPN
afs-72416	226	5	,	,	PUNCT
afs-72416	226	6	davey	davey	NOUN
afs-72416	226	7	,	,	PUNCT
afs-72416	226	8	m.r	m.r	PROPN
afs-72416	226	9	.	.	PROPN
afs-72416	226	10	,	,	PUNCT
afs-72416	226	11	cocking	cock	VERB
afs-72416	226	12	,	,	PUNCT
afs-72416	226	13	e.c	e.c	PROPN
afs-72416	226	14	.	.	PROPN
afs-72416	226	15	1982	1982	NUM
afs-72416	226	16	.	.	PUNCT
afs-72416	227	1	plant	plant	NOUN
afs-72416	227	2	regeneration	regeneration	NOUN
afs-72416	227	3	from	from	ADP
afs-72416	227	4	root	root	NOUN
afs-72416	227	5	protoplasts	protoplast	NOUN
afs-72416	227	6	of	of	ADP
afs-72416	227	7	brassica	brassica	NOUN
afs-72416	227	8	.	.	PUNCT
afs-72416	228	1	plant	plant	PROPN
afs-72416	228	2	sci	sci	PROPN
afs-72416	228	3	.	.	PUNCT
afs-72416	228	4	lett	lett	PROPN
afs-72416	228	5	.	.	PUNCT
afs-72416	229	1	24	24	NUM
afs-72416	229	2	:	:	PUNCT
afs-72416	229	3	117—121	117—121	NUM
afs-72416	229	4	.	.	PUNCT
afs-72416	230	1	ms	ms	PROPN
afs-72416	230	2	received	receive	VERB
afs-72416	230	3	june	june	PROPN
afs-72416	230	4	9	9	NUM
afs-72416	230	5	,	,	PUNCT
afs-72416	230	6	1991	1991	NUM
afs-72416	230	7	selostus	selostus	NOUN
afs-72416	230	8	maataloudelle	maataloudelle	NOUN
afs-72416	230	9	tarkeiden	tarkeiden	PROPN
afs-72416	230	10	brassica	brassica	PROPN
afs-72416	230	11	-	-	PUNCT
afs-72416	230	12	lajien	lajien	NOUN
afs-72416	230	13	protoplastien	protoplastien	NOUN
afs-72416	230	14	viljely	viljely	ADV
afs-72416	230	15	ja	ja	PROPN
afs-72416	230	16	kasvien	kasvien	PROPN
afs-72416	230	17	regenerointi	regenerointi	VERB
afs-72416	230	18	janos	janos	PROPN
afs-72416	230	19	pauk	pauk	PROPN
afs-72416	230	20	*	*	PROPN
afs-72416	230	21	,	,	PUNCT
afs-72416	230	22	sandor	sandor	PROPN
afs-72416	230	23	fekete	fekete	PROPN
afs-72416	230	24	*	*	PROPN
afs-72416	230	25	,	,	PUNCT
afs-72416	230	26	juha	juha	PROPN
afs-72416	230	27	vilkki	vilkki	PROPN
afs-72416	230	28	*	*	PUNCT
afs-72416	230	29	*	*	PUNCT
afs-72416	230	30	and	and	CCONJ
afs-72416	230	31	seppo	seppo	ADJ
afs-72416	230	32	pulli	pulli	PROPN
afs-72416	230	33	*	*	PROPN
afs-72416	230	34	*	*	PUNCT
afs-72416	230	35	•	•	NUM
afs-72416	230	36	cereal	cereal	PROPN
afs-72416	230	37	research	research	PROPN
afs-72416	230	38	institute	institute	PROPN
afs-72416	230	39	,	,	PUNCT
afs-72416	230	40	pob	pob	PROPN
afs-72416	230	41	.	.	PROPN
afs-72416	230	42	391	391	NUM
afs-72416	230	43	,	,	PUNCT
afs-72416	230	44	h-6701	h-6701	PROPN
afs-72416	230	45	szeged	szeged	PROPN
afs-72416	230	46	,	,	PUNCT
afs-72416	230	47	hungary	hungary	PROPN
afs-72416	230	48	*	*	PUNCT
afs-72416	230	49	*	*	PUNCT
afs-72416	230	50	agricultural	agricultural	ADJ
afs-72416	230	51	research	research	NOUN
afs-72416	230	52	centre	centre	NOUN
afs-72416	230	53	,	,	PUNCT
afs-72416	230	54	institute	institute	NOUN
afs-72416	230	55	of	of	ADP
afs-72416	230	56	plant	plant	NOUN
afs-72416	230	57	breeding	breeding	NOUN
afs-72416	230	58	,	,	PUNCT
afs-72416	230	59	sf-31600	sf-31600	PROPN
afs-72416	230	60	jokioinen	jokioinen	PROPN
afs-72416	230	61	,	,	PUNCT
afs-72416	230	62	finland	finland	PROPN
afs-72416	230	63	tutkimuksessa	tutkimuksessa	PROPN
afs-72416	230	64	eristettiin	eristettiin	VERB
afs-72416	230	65	protoplasteja	protoplasteja	PROPN
afs-72416	230	66	kolmentoista	kolmentoista	PROPN
afs-72416	230	67	rapsilajikkeen	rapsilajikkeen	PROPN
afs-72416	230	68	(	(	PUNCT
afs-72416	230	69	brassica	brassica	PROPN
afs-72416	230	70	napus	napus	PROPN
afs-72416	230	71	)	)	PUNCT
afs-72416	230	72	ja	ja	PROPN
afs-72416	230	73	yhdenkevatrypsilinjan	yhdenkevatrypsilinjan	PROPN
afs-72416	231	1	(	(	PUNCT
afs-72416	231	2	b.	b.	PROPN
afs-72416	231	3	campestris	campestris	PROPN
afs-72416	231	4	)	)	PUNCT
afs-72416	231	5	6	6	NUM
afs-72416	231	6	vrk	vrk	NOUN
afs-72416	231	7	:	:	PUNCT
afs-72416	231	8	n	n	PRON
afs-72416	231	9	ikaisista	ikaisista	ADJ
afs-72416	231	10	hypokotyyleista	hypokotyyleista	NOUN
afs-72416	231	11	.	.	PUNCT
afs-72416	232	1	protoplastien	protoplastien	PROPN
afs-72416	232	2	eristyksessa	eristyksessa	ADJ
afs-72416	232	3	kaytettiin	kaytettiin	PROPN
afs-72416	232	4	tavanomaista	tavanomaista	PROPN
afs-72416	232	5	korkeampimolaarista	korkeampimolaarista	PROPN
afs-72416	232	6	entsyymiliuosta	entsyymiliuosta	PROPN
afs-72416	232	7	(	(	PUNCT
afs-72416	232	8	0.714	0.714	NUM
afs-72416	232	9	m	m	NOUN
afs-72416	232	10	mannitoli	mannitoli	NOUN
afs-72416	232	11	)	)	PUNCT
afs-72416	232	12	.	.	PUNCT
afs-72416	233	1	main	main	ADJ
afs-72416	233	2	saatiin	saatiin	NOUN
afs-72416	233	3	pienia	pienia	NOUN
afs-72416	233	4	,	,	PUNCT
afs-72416	233	5	kestavia	kestavia	PROPN
afs-72416	233	6	,	,	PUNCT
afs-72416	233	7	runsaasti	runsaasti	NOUN
afs-72416	233	8	solulimaa	solulimaa	VERB
afs-72416	233	9	sisaltavia	sisaltavia	PROPN
afs-72416	233	10	protoplasteja	protoplasteja	PROPN
afs-72416	233	11	.	.	PUNCT
afs-72416	234	1	protoplastien	protoplastien	PROPN
afs-72416	234	2	vaurioitumisen	vaurioitumisen	PROPN
afs-72416	234	3	valttamiseksi	valttamiseksi	PROPN
afs-72416	234	4	eristyksessa	eristyksessa	PROPN
afs-72416	234	5	kaytettiin	kaytettiin	PROPN
afs-72416	234	6	tiheysgradienttisentrifugointia	tiheysgradienttisentrifugointia	PROPN
afs-72416	234	7	.	.	PUNCT
afs-72416	235	1	tutkimuksessa	tutkimuksessa	PROPN
afs-72416	235	2	verrattiin	verrattiin	NOUN
afs-72416	235	3	kolmea	kolmea	PROPN
afs-72416	235	4	eri	eri	PROPN
afs-72416	235	5	protoplastiviljelymenetelmaa	protoplastiviljelymenetelmaa	PROPN
afs-72416	235	6	:	:	PUNCT
afs-72416	235	7	1)nesteviljely	1)nesteviljely	ADV
afs-72416	235	8	,	,	PUNCT
afs-72416	235	9	2	2	X
afs-72416	235	10	)	)	PUNCT
afs-72416	235	11	maljaus	maljaus	PROPN
afs-72416	235	12	agaroosiin	agaroosiin	NOUN
afs-72416	236	1	toisena	toisena	PROPN
afs-72416	236	2	viljelypaivana	viljelypaivana	PROPN
afs-72416	236	3	,	,	PUNCT
afs-72416	236	4	3	3	X
afs-72416	236	5	)	)	PUNCT
afs-72416	236	6	suora	suora	VERB
afs-72416	236	7	maljaus	maljaus	PROPN
afs-72416	236	8	agaroosiin	agaroosiin	PROPN
afs-72416	236	9	.	.	PUNCT
afs-72416	237	1	agaroosiviljelmissa	agaroosiviljelmissa	PROPN
afs-72416	237	2	solut	solut	PROPN
afs-72416	237	3	jakautuivat	jakautuivat	PROPN
afs-72416	237	4	yhta	yhta	PROPN
afs-72416	237	5	aktiivisesti	aktiivisesti	PROPN
afs-72416	237	6	kuin	kuin	NOUN
afs-72416	237	7	nesteviljelyssa	nesteviljelyssa	NOUN
afs-72416	237	8	,	,	PUNCT
afs-72416	237	9	lisaksi	lisaksi	PROPN
afs-72416	237	10	valtyttiin	valtyttiin	X
afs-72416	237	11	solukolonioiden	solukolonioiden	ADJ
afs-72416	237	12	ruskettumiselta	ruskettumiselta	NOUN
afs-72416	237	13	.	.	PUNCT
afs-72416	238	1	hopeanitraatin	hopeanitraatin	NOUN
afs-72416	238	2	(	(	PUNCT
afs-72416	238	3	agng3	agng3	X
afs-72416	238	4	)	)	PUNCT
afs-72416	238	5	kaytto	kaytto	NOUN
afs-72416	238	6	regeneraatioalustassa	regeneraatioalustassa	NOUN
afs-72416	238	7	(	(	PUNCT
afs-72416	238	8	5	5	NUM
afs-72416	238	9	mg/1	mg/1	NOUN
afs-72416	238	10	)	)	PUNCT
afs-72416	238	11	lisasi	lisasi	PROPN
afs-72416	238	12	kalluksesta	kalluksesta	PROPN
afs-72416	238	13	muodostuvien	muodostuvien	PROPN
afs-72416	238	14	versojen	versojen	PROPN
afs-72416	238	15	maaraa	maaraa	NOUN
afs-72416	238	16	niilla	niilla	ADV
afs-72416	238	17	10	10	NUM
afs-72416	238	18	lajikkeella	lajikkeella	ADJ
afs-72416	238	19	,	,	PUNCT
afs-72416	238	20	joilla	joilla	PROPN
afs-72416	238	21	havaittiin	havaittiin	VERB
afs-72416	238	22	vahaista	vahaista	PROPN
afs-72416	238	23	regeneraatiota	regeneraatiota	PROPN
afs-72416	238	24	jo	jo	PROPN
afs-72416	238	25	ilman	ilman	PROPN
afs-72416	238	26	hopeanitraattia	hopeanitraattia	PROPN
afs-72416	238	27	.	.	PUNCT
afs-72416	239	1	kolmella	kolmella	PROPN
afs-72416	239	2	lajikkeella	lajikkeella	PROPN
afs-72416	239	3	regeneraatio	regeneraatio	PROPN
afs-72416	239	4	onnistui	onnistui	PROPN
afs-72416	239	5	ainoastaan	ainoastaan	PROPN
afs-72416	239	6	silloin	silloin	VERB
afs-72416	239	7	kuin	kuin	NOUN
afs-72416	239	8	regeneraatioalustassa	regeneraatioalustassa	PROPN
afs-72416	239	9	oli	oli	PROPN
afs-72416	239	10	hopeanitraattia	hopeanitraattia	PROPN
afs-72416	239	11	.	.	PUNCT
afs-72416	240	1	erityisen	erityisen	PROPN
afs-72416	240	2	suotuisa	suotuisa	NOUN
afs-72416	240	3	vaikutus	vaikutus	PROPN
afs-72416	240	4	hopeanitraattilisayksella	hopeanitraattilisayksella	PROPN
afs-72416	240	5	oli	oli	PROPN
afs-72416	240	6	kevatrypsin	kevatrypsin	PROPN
afs-72416	240	7	regeneraatioon	regeneraatioon	NOUN
afs-72416	240	8	.	.	PUNCT
afs-72416	241	1	378	378	NUM
