id	sid	tid	token	lemma	pos
afs-72443	1	1	sex	sex	NOUN
afs-72443	1	2	diagnosis	diagnosis	NOUN
afs-72443	1	3	of	of	ADP
afs-72443	1	4	ovine	ovine	ADJ
afs-72443	1	5	and	and	CCONJ
afs-72443	1	6	bovine	bovine	ADJ
afs-72443	1	7	embryos	embryo	NOUN
afs-72443	1	8	by	by	ADP
afs-72443	1	9	enzymatic	enzymatic	ADJ
afs-72443	1	10	amplification	amplification	NOUN
afs-72443	1	11	and	and	CCONJ
afs-72443	1	12	digestion	digestion	NOUN
afs-72443	1	13	of	of	ADP
afs-72443	1	14	dna	dna	NOUN
afs-72443	1	15	from	from	ADP
afs-72443	1	16	the	the	DET
afs-72443	1	17	zfy	zfy	PROPN
afs-72443	1	18	/	/	SYM
afs-72443	1	19	zfx	zfx	NOUN
afs-72443	1	20	locus	locus	PROPN
afs-72443	1	21	peter	peter	PROPN
afs-72443	1	22	bredbacka	bredbacka	PROPN
afs-72443	1	23	and	and	CCONJ
afs-72443	1	24	jaana	jaana	PROPN
afs-72443	1	25	peippo	peippo	PROPN
afs-72443	1	26	bredbacka	bredbacka	PROPN
afs-72443	1	27	,	,	PUNCT
afs-72443	1	28	p.	p.	PROPN
afs-72443	1	29	&	&	CCONJ
afs-72443	1	30	peippo	peippo	PROPN
afs-72443	1	31	,	,	PUNCT
afs-72443	1	32	j.	j.	PROPN
afs-72443	1	33	1992	1992	NUM
afs-72443	1	34	.	.	PUNCT
afs-72443	2	1	sex	sex	NOUN
afs-72443	2	2	diagnosis	diagnosis	NOUN
afs-72443	2	3	of	of	ADP
afs-72443	2	4	ovine	ovine	ADJ
afs-72443	2	5	and	and	CCONJ
afs-72443	2	6	bovine	bovine	ADJ
afs-72443	2	7	embryos	embryo	NOUN
afs-72443	2	8	by	by	ADP
afs-72443	2	9	enzymatic	enzymatic	ADJ
afs-72443	2	10	amplification	amplification	NOUN
afs-72443	2	11	and	and	CCONJ
afs-72443	2	12	digestion	digestion	NOUN
afs-72443	2	13	of	of	ADP
afs-72443	2	14	dna	dna	NOUN
afs-72443	2	15	from	from	ADP
afs-72443	2	16	the	the	DET
afs-72443	2	17	zfy	zfy	PROPN
afs-72443	2	18	/	/	SYM
afs-72443	2	19	zfx	zfx	NOUN
afs-72443	2	20	locus	locus	NOUN
afs-72443	2	21	.	.	PUNCT
afs-72443	3	1	agric	agric	PROPN
afs-72443	3	2	.	.	PUNCT
afs-72443	4	1	sei	sei	PROPN
afs-72443	4	2	.	.	PUNCT
afs-72443	4	3	finl	finl	NOUN
afs-72443	4	4	.	.	PUNCT
afs-72443	5	1	1	1	NUM
afs-72443	5	2	:	:	SYM
afs-72443	5	3	233	233	NUM
afs-72443	5	4	-	-	SYM
afs-72443	5	5	238	238	NUM
afs-72443	5	6	.	.	PUNCT
afs-72443	6	1	(	(	PUNCT
afs-72443	6	2	agric	agric	PROPN
afs-72443	6	3	.	.	PUNCT
afs-72443	7	1	res	res	PROPN
afs-72443	7	2	.	.	PUNCT
afs-72443	7	3	centre	centre	PROPN
afs-72443	7	4	of	of	ADP
afs-72443	7	5	finland	finland	PROPN
afs-72443	7	6	.	.	PUNCT
afs-72443	7	7	,	,	PUNCT
afs-72443	7	8	dept	dept	PROPN
afs-72443	7	9	.	.	PROPN
afs-72443	7	10	anim	anim	PROPN
afs-72443	7	11	,	,	PUNCT
afs-72443	7	12	breed	breed	NOUN
afs-72443	7	13	.	.	PUNCT
afs-72443	7	14	,	,	PUNCT
afs-72443	7	15	sf-31600	sf-31600	PROPN
afs-72443	7	16	jokioinen	jokioinen	PROPN
afs-72443	7	17	,	,	PUNCT
afs-72443	7	18	finland	finland	PROPN
afs-72443	7	19	.	.	PUNCT
afs-72443	7	20	)	)	PUNCT
afs-72443	8	1	a	a	DET
afs-72443	8	2	pcr	pcr	NOUN
afs-72443	8	3	-	-	PUNCT
afs-72443	8	4	based	base	VERB
afs-72443	8	5	sex	sex	NOUN
afs-72443	8	6	determination	determination	NOUN
afs-72443	8	7	assay	assay	VERB
afs-72443	8	8	for	for	ADP
afs-72443	8	9	sheep	sheep	NOUN
afs-72443	8	10	and	and	CCONJ
afs-72443	8	11	cattle	cattle	NOUN
afs-72443	8	12	embryos	embryo	NOUN
afs-72443	8	13	was	be	AUX
afs-72443	8	14	developed	develop	VERB
afs-72443	8	15	using	use	VERB
afs-72443	8	16	mouse	mouse	NOUN
afs-72443	8	17	embryos	embryo	NOUN
afs-72443	8	18	for	for	ADP
afs-72443	8	19	optimizing	optimize	VERB
afs-72443	8	20	the	the	DET
afs-72443	8	21	protocol	protocol	NOUN
afs-72443	8	22	.	.	PUNCT
afs-72443	9	1	samples	sample	NOUN
afs-72443	9	2	were	be	AUX
afs-72443	9	3	lysed	lyse	VERB
afs-72443	9	4	either	either	CCONJ
afs-72443	9	5	enzymatically	enzymatically	ADV
afs-72443	9	6	or	or	CCONJ
afs-72443	9	7	by	by	ADP
afs-72443	9	8	alkaline	alkaline	NOUN
afs-72443	9	9	treatment	treatment	NOUN
afs-72443	9	10	followed	follow	VERB
afs-72443	9	11	by	by	ADP
afs-72443	9	12	enzymatic	enzymatic	ADJ
afs-72443	9	13	amplification	amplification	NOUN
afs-72443	9	14	of	of	ADP
afs-72443	9	15	dna	dna	NOUN
afs-72443	9	16	from	from	ADP
afs-72443	9	17	the	the	DET
afs-72443	9	18	zfy	zfy	PROPN
afs-72443	9	19	/	/	SYM
afs-72443	9	20	zfx	zfx	NOUN
afs-72443	9	21	locus	locus	NOUN
afs-72443	9	22	.	.	PUNCT
afs-72443	10	1	sex	sex	NOUN
afs-72443	10	2	diagnosis	diagnosis	NOUN
afs-72443	10	3	could	could	AUX
afs-72443	10	4	be	be	AUX
afs-72443	10	5	done	do	VERB
afs-72443	10	6	after	after	ADP
afs-72443	10	7	the	the	DET
afs-72443	10	8	digestion	digestion	NOUN
afs-72443	10	9	of	of	ADP
afs-72443	10	10	the	the	DET
afs-72443	10	11	amplified	amplify	VERB
afs-72443	10	12	product	product	NOUN
afs-72443	10	13	by	by	ADP
afs-72443	10	14	restriction	restriction	NOUN
afs-72443	10	15	endonucleases	endonuclease	NOUN
afs-72443	10	16	.	.	PUNCT
afs-72443	11	1	ovine	ovine	ADJ
afs-72443	11	2	and	and	CCONJ
afs-72443	11	3	bovine	bovine	ADJ
afs-72443	11	4	embryos	embryo	NOUN
afs-72443	11	5	could	could	AUX
afs-72443	11	6	be	be	AUX
afs-72443	11	7	sexed	sex	VERB
afs-72443	11	8	from	from	ADP
afs-72443	11	9	biopsies	biopsy	NOUN
afs-72443	11	10	as	as	ADV
afs-72443	11	11	small	small	ADJ
afs-72443	11	12	as	as	ADP
afs-72443	11	13	1	1	NUM
afs-72443	11	14	-	-	SYM
afs-72443	11	15	4	4	NUM
afs-72443	11	16	cells	cell	NOUN
afs-72443	11	17	.	.	PUNCT
afs-72443	12	1	some	some	DET
afs-72443	12	2	embryos	embryo	NOUN
afs-72443	12	3	were	be	AUX
afs-72443	12	4	split	split	VERB
afs-72443	12	5	into	into	ADP
afs-72443	12	6	2	2	NUM
afs-72443	12	7	-	-	SYM
afs-72443	12	8	4	4	NUM
afs-72443	12	9	sections	section	NOUN
afs-72443	12	10	,	,	PUNCT
afs-72443	12	11	which	which	PRON
afs-72443	12	12	were	be	AUX
afs-72443	12	13	amplified	amplify	VERB
afs-72443	12	14	separately	separately	ADV
afs-72443	12	15	.	.	PUNCT
afs-72443	13	1	blind	blind	ADJ
afs-72443	13	2	trials	trial	NOUN
afs-72443	13	3	with	with	ADP
afs-72443	13	4	such	such	ADJ
afs-72443	13	5	samples	sample	NOUN
afs-72443	13	6	demonstrated	demonstrate	VERB
afs-72443	13	7	that	that	SCONJ
afs-72443	13	8	the	the	DET
afs-72443	13	9	method	method	NOUN
afs-72443	13	10	was	be	AUX
afs-72443	13	11	highly	highly	ADV
afs-72443	13	12	accurate	accurate	ADJ
afs-72443	13	13	,	,	PUNCT
afs-72443	13	14	even	even	ADV
afs-72443	13	15	when	when	SCONJ
afs-72443	13	16	embryo	embryo	NOUN
afs-72443	13	17	biopsy	biopsy	NOUN
afs-72443	13	18	was	be	AUX
afs-72443	13	19	done	do	VERB
afs-72443	13	20	under	under	ADP
afs-72443	13	21	farm	farm	NOUN
afs-72443	13	22	conditions	condition	NOUN
afs-72443	13	23	.	.	PUNCT
afs-72443	14	1	the	the	DET
afs-72443	14	2	protocol	protocol	NOUN
afs-72443	14	3	involves	involve	VERB
afs-72443	14	4	an	an	DET
afs-72443	14	5	in	in	ADV
afs-72443	14	6	-	-	PUNCT
afs-72443	14	7	built	build	VERB
afs-72443	14	8	control	control	NOUN
afs-72443	14	9	.	.	PUNCT
afs-72443	15	1	this	this	PRON
afs-72443	15	2	eliminates	eliminate	VERB
afs-72443	15	3	the	the	DET
afs-72443	15	4	need	need	NOUN
afs-72443	15	5	for	for	ADP
afs-72443	15	6	autosomal	autosomal	ADJ
afs-72443	15	7	control	control	NOUN
afs-72443	15	8	primers	primer	NOUN
afs-72443	15	9	,	,	PUNCT
afs-72443	15	10	which	which	PRON
afs-72443	15	11	often	often	ADV
afs-72443	15	12	inhibit	inhibit	VERB
afs-72443	15	13	the	the	DET
afs-72443	15	14	amplification	amplification	NOUN
afs-72443	15	15	of	of	ADP
afs-72443	15	16	the	the	DET
afs-72443	15	17	y	y	PROPN
afs-72443	15	18	-	-	PUNCT
afs-72443	15	19	chromosome	chromosome	NOUN
afs-72443	15	20	-	-	PUNCT
afs-72443	15	21	specific	specific	ADJ
afs-72443	15	22	dna	dna	NOUN
afs-72443	15	23	,	,	PUNCT
afs-72443	15	24	especially	especially	ADV
afs-72443	15	25	when	when	SCONJ
afs-72443	15	26	a	a	DET
afs-72443	15	27	small	small	ADJ
afs-72443	15	28	amount	amount	NOUN
afs-72443	15	29	of	of	ADP
afs-72443	15	30	template	template	NOUN
afs-72443	15	31	is	be	AUX
afs-72443	15	32	used	use	VERB
afs-72443	15	33	.	.	PUNCT
afs-72443	16	1	key	key	ADJ
afs-72443	16	2	words	word	NOUN
afs-72443	16	3	:	:	PUNCT
afs-72443	16	4	sexing	sex	VERB
afs-72443	16	5	,	,	PUNCT
afs-72443	16	6	embryo	embryo	NOUN
afs-72443	16	7	,	,	PUNCT
afs-72443	16	8	sheep	sheep	NOUN
afs-72443	16	9	,	,	PUNCT
afs-72443	16	10	cattle	cattle	NOUN
afs-72443	16	11	,	,	PUNCT
afs-72443	16	12	polymerase	polymerase	NOUN
afs-72443	16	13	chain	chain	NOUN
afs-72443	16	14	reaction	reaction	NOUN
afs-72443	16	15	,	,	PUNCT
afs-72443	16	16	restriction	restriction	NOUN
afs-72443	16	17	fragment	fragment	NOUN
afs-72443	16	18	length	length	NOUN
afs-72443	16	19	polymorphism	polymorphism	NOUN
afs-72443	16	20	introduction	introduction	NOUN
afs-72443	16	21	the	the	DET
afs-72443	16	22	production	production	NOUN
afs-72443	16	23	ofoffspring	ofoffspring	NOUN
afs-72443	16	24	of	of	ADP
afs-72443	16	25	the	the	DET
afs-72443	16	26	desired	desire	VERB
afs-72443	16	27	sex	sex	NOUN
afs-72443	16	28	from	from	ADP
afs-72443	16	29	livestock	livestock	NOUN
afs-72443	16	30	species	specie	NOUN
afs-72443	16	31	is	be	AUX
afs-72443	16	32	becoming	become	VERB
afs-72443	16	33	a	a	DET
afs-72443	16	34	reality	reality	NOUN
afs-72443	16	35	.	.	PUNCT
afs-72443	17	1	although	although	SCONJ
afs-72443	17	2	the	the	DET
afs-72443	17	3	method	method	NOUN
afs-72443	17	4	of	of	ADP
afs-72443	17	5	choice	choice	NOUN
afs-72443	17	6	wouldbe	wouldbe	VERB
afs-72443	17	7	the	the	DET
afs-72443	17	8	separation	separation	NOUN
afs-72443	17	9	of	of	ADP
afs-72443	17	10	xand	xand	PROPN
afs-72443	17	11	y	y	PROPN
afs-72443	17	12	-	-	PUNCT
afs-72443	17	13	chromosome	chromosome	NOUN
afs-72443	17	14	-	-	PUNCT
afs-72443	17	15	bearing	bear	VERB
afs-72443	17	16	sperm	sperm	NOUN
afs-72443	17	17	prior	prior	ADV
afs-72443	17	18	to	to	ADP
afs-72443	17	19	ai	ai	PROPN
afs-72443	17	20	,	,	PUNCT
afs-72443	17	21	the	the	DET
afs-72443	17	22	only	only	ADJ
afs-72443	17	23	application	application	NOUN
afs-72443	17	24	that	that	PRON
afs-72443	17	25	can	can	AUX
afs-72443	17	26	be	be	AUX
afs-72443	17	27	used	use	VERB
afs-72443	17	28	in	in	ADP
afs-72443	17	29	practical	practical	ADJ
afs-72443	17	30	conditions	condition	NOUN
afs-72443	17	31	at	at	ADP
afs-72443	17	32	present	present	NOUN
afs-72443	17	33	is	be	AUX
afs-72443	17	34	to	to	PART
afs-72443	17	35	sex	sex	VERB
afs-72443	17	36	embryos	embryo	NOUN
afs-72443	17	37	in	in	ADP
afs-72443	17	38	connection	connection	NOUN
afs-72443	17	39	with	with	ADP
afs-72443	17	40	embryo	embryo	NOUN
afs-72443	17	41	transfer	transfer	NOUN
afs-72443	17	42	.	.	PUNCT
afs-72443	18	1	various	various	ADJ
afs-72443	18	2	approaches	approach	NOUN
afs-72443	18	3	have	have	AUX
afs-72443	18	4	been	be	AUX
afs-72443	18	5	used	use	VERB
afs-72443	18	6	to	to	PART
afs-72443	18	7	determine	determine	VERB
afs-72443	18	8	the	the	DET
afs-72443	18	9	sex	sex	NOUN
afs-72443	18	10	of	of	ADP
afs-72443	18	11	embryos	embryo	NOUN
afs-72443	18	12	(	(	PUNCT
afs-72443	18	13	for	for	ADP
afs-72443	18	14	a	a	DET
afs-72443	18	15	review	review	NOUN
afs-72443	18	16	,	,	PUNCT
afs-72443	18	17	see	see	VERB
afs-72443	18	18	van	van	PROPN
afs-72443	18	19	vliet	vliet	PROPN
afs-72443	18	20	et	et	PROPN
afs-72443	18	21	al	al	PROPN
afs-72443	18	22	.	.	PROPN
afs-72443	18	23	1989	1989	NUM
afs-72443	18	24	)	)	PUNCT
afs-72443	18	25	,	,	PUNCT
afs-72443	18	26	and	and	CCONJ
afs-72443	18	27	at	at	ADP
afs-72443	18	28	present	present	ADJ
afs-72443	18	29	the	the	DET
afs-72443	18	30	technique	technique	NOUN
afs-72443	18	31	utilizing	utilize	VERB
afs-72443	18	32	the	the	DET
afs-72443	18	33	polymerase	polymerase	NOUN
afs-72443	18	34	chain	chain	NOUN
afs-72443	18	35	reaction	reaction	NOUN
afs-72443	18	36	(	(	PUNCT
afs-72443	18	37	pcr	pcr	PROPN
afs-72443	18	38	)	)	PUNCT
afs-72443	18	39	seems	seem	VERB
afs-72443	18	40	to	to	PART
afs-72443	18	41	be	be	AUX
afs-72443	18	42	the	the	DET
afs-72443	18	43	most	most	ADV
afs-72443	18	44	effective	effective	ADJ
afs-72443	18	45	one	one	NUM
afs-72443	18	46	.	.	PUNCT
afs-72443	19	1	pcr	pcr	PROPN
afs-72443	19	2	involves	involve	VERB
afs-72443	19	3	the	the	DET
afs-72443	19	4	enzymatic	enzymatic	ADJ
afs-72443	19	5	amplification	amplification	NOUN
afs-72443	19	6	of	of	ADP
afs-72443	19	7	dna	dna	NOUN
afs-72443	19	8	with	with	ADP
afs-72443	19	9	the	the	DET
afs-72443	19	10	help	help	NOUN
afs-72443	19	11	of	of	ADP
afs-72443	19	12	oligonucleotide	oligonucleotide	VERB
afs-72443	19	13	primers	primer	NOUN
afs-72443	19	14	(	(	PUNCT
afs-72443	19	15	saiki	saiki	PROPN
afs-72443	19	16	et	et	PROPN
afs-72443	19	17	al	al	PROPN
afs-72443	19	18	.	.	PROPN
afs-72443	19	19	1985	1985	NUM
afs-72443	19	20	)	)	PUNCT
afs-72443	19	21	.	.	PUNCT
afs-72443	20	1	by	by	ADP
afs-72443	20	2	annealing	anneal	VERB
afs-72443	20	3	to	to	ADP
afs-72443	20	4	specific	specific	ADJ
afs-72443	20	5	sites	site	NOUN
afs-72443	20	6	of	of	ADP
afs-72443	20	7	the	the	DET
afs-72443	20	8	genomic	genomic	NOUN
afs-72443	20	9	dna	dna	NOUN
afs-72443	20	10	,	,	PUNCT
afs-72443	20	11	the	the	DET
afs-72443	20	12	primers	primer	NOUN
afs-72443	20	13	define	define	VERB
afs-72443	20	14	the	the	DET
afs-72443	20	15	initiation	initiation	NOUN
afs-72443	20	16	site	site	NOUN
afs-72443	20	17	of	of	ADP
afs-72443	20	18	dna	dna	PROPN
afs-72443	20	19	synthesis	synthesis	NOUN
afs-72443	20	20	.	.	PUNCT
afs-72443	21	1	when	when	SCONJ
afs-72443	21	2	primers	primer	NOUN
afs-72443	21	3	anneal	anneal	VERB
afs-72443	21	4	on	on	ADP
afs-72443	21	5	opposite	opposite	ADJ
afs-72443	21	6	dna	dna	PROPN
afs-72443	21	7	strands	strand	NOUN
afs-72443	21	8	,	,	PUNCT
afs-72443	21	9	the	the	DET
afs-72443	21	10	intervening	intervene	VERB
afs-72443	21	11	dna	dna	NOUN
afs-72443	21	12	sequence	sequence	NOUN
afs-72443	21	13	can	can	AUX
afs-72443	21	14	be	be	AUX
afs-72443	21	15	amplified	amplify	VERB
afs-72443	21	16	in	in	ADP
afs-72443	21	17	an	an	DET
afs-72443	21	18	exponential	exponential	ADJ
afs-72443	21	19	manner	manner	NOUN
afs-72443	21	20	.	.	PUNCT
afs-72443	22	1	this	this	PRON
afs-72443	22	2	is	be	AUX
afs-72443	22	3	possible	possible	ADJ
afs-72443	22	4	by	by	ADP
afs-72443	22	5	repeating	repeat	VERB
afs-72443	22	6	each	each	DET
afs-72443	22	7	amplification	amplification	NOUN
afs-72443	22	8	cycle	cycle	NOUN
afs-72443	22	9	,	,	PUNCT
afs-72443	22	10	which	which	PRON
afs-72443	22	11	involves	involve	VERB
afs-72443	22	12	heat	heat	NOUN
afs-72443	22	13	denaturation	denaturation	NOUN
afs-72443	22	14	of	of	ADP
afs-72443	22	15	the	the	DET
afs-72443	22	16	dna	dna	PROPN
afs-72443	22	17	,	,	PUNCT
afs-72443	22	18	primer	primer	NOUN
afs-72443	22	19	annealing	annealing	NOUN
afs-72443	22	20	and	and	CCONJ
afs-72443	22	21	dna	dna	PROPN
afs-72443	22	22	synthesis	synthesis	NOUN
afs-72443	22	23	.	.	PUNCT
afs-72443	23	1	the	the	DET
afs-72443	23	2	emergence	emergence	NOUN
afs-72443	23	3	of	of	ADP
afs-72443	23	4	thermostabile	thermostabile	NOUN
afs-72443	23	5	polymerases	polymerase	NOUN
afs-72443	23	6	for	for	ADP
afs-72443	23	7	dna	dna	NOUN
afs-72443	23	8	synthesis	synthesis	NOUN
afs-72443	23	9	(	(	PUNCT
afs-72443	23	10	saiki	saiki	PROPN
afs-72443	23	11	et	et	PROPN
afs-72443	23	12	al	al	PROPN
afs-72443	23	13	.	.	PROPN
afs-72443	23	14	1988	1988	NUM
afs-72443	23	15	)	)	PUNCT
afs-72443	23	16	has	have	AUX
afs-72443	23	17	greatly	greatly	ADV
afs-72443	23	18	increased	increase	VERB
afs-72443	23	19	the	the	DET
afs-72443	23	20	applicability	applicability	NOUN
afs-72443	23	21	ofpcr	ofpcr	NOUN
afs-72443	23	22	.	.	PUNCT
afs-72443	24	1	the	the	DET
afs-72443	24	2	greater	great	ADJ
afs-72443	24	3	speed	speed	NOUN
afs-72443	24	4	has	have	AUX
afs-72443	24	5	made	make	VERB
afs-72443	24	6	it	it	PRON
afs-72443	24	7	possible	possible	ADJ
afs-72443	24	8	to	to	ADP
afs-72443	24	9	sex	sex	VERB
afs-72443	24	10	embryos	embryo	NOUN
afs-72443	24	11	on	on	ADP
afs-72443	24	12	the	the	DET
afs-72443	24	13	same	same	ADJ
afs-72443	24	14	day	day	NOUN
afs-72443	24	15	as	as	ADP
afs-72443	24	16	embryo	embryo	NOUN
afs-72443	24	17	recovery	recovery	NOUN
afs-72443	24	18	and	and	CCONJ
afs-72443	24	19	transfer	transfer	NOUN
afs-72443	24	20	are	be	AUX
afs-72443	24	21	carried	carry	VERB
afs-72443	24	22	out	out	ADP
afs-72443	24	23	.	.	PUNCT
afs-72443	25	1	several	several	ADJ
afs-72443	25	2	authors	author	NOUN
afs-72443	25	3	have	have	AUX
afs-72443	25	4	reported	report	VERB
afs-72443	25	5	successful	successful	ADJ
afs-72443	25	6	amplification	amplification	NOUN
afs-72443	25	7	of	of	ADP
afs-72443	25	8	male	male	NOUN
afs-72443	25	9	-	-	PUNCT
afs-72443	25	10	specific	specific	ADJ
afs-72443	25	11	dna	dna	NOUN
afs-72443	25	12	from	from	ADP
afs-72443	25	13	embryo	embryo	NOUN
afs-72443	25	14	biopsies	biopsy	NOUN
afs-72443	25	15	(	(	PUNCT
afs-72443	25	16	handyside	handyside	PROPN
afs-72443	25	17	et	et	PROPN
afs-72443	25	18	al	al	PROPN
afs-72443	25	19	.	.	PROPN
afs-72443	25	20	1989	1989	NUM
afs-72443	25	21	,	,	PUNCT
afs-72443	25	22	bradbury	bradbury	PROPN
afs-72443	25	23	et	et	PROPN
afs-72443	25	24	al	al	PROPN
afs-72443	25	25	.	.	PROPN
afs-72443	25	26	1990	1990	NUM
afs-72443	25	27	,	,	PUNCT
afs-72443	25	28	herr	herr	PROPN
afs-72443	25	29	et	et	PROPN
afs-72443	25	30	al	al	PROPN
afs-72443	25	31	.	.	PROPN
afs-72443	25	32	1990	1990	NUM
afs-72443	25	33	a	a	DET
afs-72443	25	34	,	,	PUNCT
afs-72443	25	35	b	b	NOUN
afs-72443	25	36	,	,	PUNCT
afs-72443	25	37	schröder	schröder	NOUN
afs-72443	25	38	et	et	PROPN
afs-72443	25	39	al	al	PROPN
afs-72443	25	40	.	.	PROPN
afs-72443	25	41	1990	1990	NUM
afs-72443	25	42	,	,	PUNCT
afs-72443	25	43	bredbacka	bredbacka	PROPN
afs-72443	25	44	et	et	PROPN
afs-72443	25	45	al	al	PROPN
afs-72443	25	46	.	.	PROPN
afs-72443	25	47	1991	1991	NUM
afs-72443	25	48	,	,	PUNCT
afs-72443	25	49	peura	peura	PROPN
afs-72443	25	50	et	et	PROPN
afs-72443	25	51	al	al	PROPN
afs-72443	25	52	.	.	PROPN
afs-72443	25	53	1991	1991	NUM
afs-72443	25	54	,	,	PUNCT
afs-72443	25	55	setiabudi	setiabudi	ADJ
afs-72443	25	56	and	and	CCONJ
afs-72443	25	57	gustavsson	gustavsson	PROPN
afs-72443	25	58	1991	1991	NUM
afs-72443	25	59	)	)	PUNCT
afs-72443	25	60	,	,	PUNCT
afs-72443	25	61	but	but	CCONJ
afs-72443	25	62	further	further	ADJ
afs-72443	25	63	development	development	NOUN
afs-72443	25	64	is	be	AUX
afs-72443	25	65	needed	need	VERB
afs-72443	25	66	to	to	PART
afs-72443	25	67	make	make	VERB
afs-72443	25	68	embryo	embryo	NOUN
afs-72443	25	69	sexing	sex	VERB
afs-72443	25	70	a	a	DET
afs-72443	25	71	more	more	ADV
afs-72443	25	72	straightforward	straightforward	ADJ
afs-72443	25	73	and	and	CCONJ
afs-72443	25	74	a	a	DET
afs-72443	25	75	more	more	ADV
afs-72443	25	76	reproducible	reproducible	ADJ
afs-72443	25	77	technique	technique	NOUN
afs-72443	25	78	.	.	PUNCT
afs-72443	26	1	233	233	NUM
afs-72443	26	2	agric	agric	NOUN
afs-72443	26	3	.	.	PUNCT
afs-72443	27	1	sei	sei	ADJ
afs-72443	27	2	.	.	PUNCT
afs-72443	27	3	finl	finl	NOUN
afs-72443	27	4	.	.	PUNCT
afs-72443	28	1	2(1992	2(1992	X
afs-72443	28	2	)	)	PUNCT
afs-72443	28	3	https://www.c-info.fi/en/info/?token=kcssegrucelcw4az.obb_nqmdiikl74ia3lxsnq.h50ts8jajhnqivoi2e6r4ekeiumxheciz9te5blqxnv066dpwzstbs8cld94e0iu96v6arjut1tkf8uekioocxc7jub5j5veopudkvyssvhjvs9ddcp9uvth84y-rukwws2bssnc2dc-kpgtarjh_5majmrppf66hkqitukqf1-jncylhgys-bklvjmsyriiyjbayacu6tro7z8eep7asrd6dmwzgkr8vnkxuu2xa_n0okwvdo3odxercc1vefz2_jjufxyc5ozfdiw9knqlqmbqpaevdoq	https://www.c-info.fi/en/info/?token=kcssegrucelcw4az.obb_nqmdiikl74ia3lxsnq.h50ts8jajhnqivoi2e6r4ekeiumxheciz9te5blqxnv066dpwzstbs8cld94e0iu96v6arjut1tkf8uekioocxc7jub5j5veopudkvyssvhjvs9ddcp9uvth84y-rukwws2bssnc2dc-kpgtarjh_5majmrppf66hkqitukqf1-jncylhgys-bklvjmsyriiyjbayacu6tro7z8eep7asrd6dmwzgkr8vnkxuu2xa_n0okwvdo3odxercc1vefz2_jjufxyc5ozfdiw9knqlqmbqpaevdoq	NOUN
afs-72443	28	4	the	the	DET
afs-72443	28	5	lack	lack	NOUN
afs-72443	28	6	of	of	ADP
afs-72443	28	7	male	male	NOUN
afs-72443	28	8	-	-	PUNCT
afs-72443	28	9	specific	specific	ADJ
afs-72443	28	10	amplification	amplification	NOUN
afs-72443	28	11	product	product	NOUN
afs-72443	28	12	can	can	AUX
afs-72443	28	13	be	be	AUX
afs-72443	28	14	caused	cause	VERB
afs-72443	28	15	either	either	CCONJ
afs-72443	28	16	by	by	ADP
afs-72443	28	17	a	a	DET
afs-72443	28	18	female	female	ADJ
afs-72443	28	19	biopsy	biopsy	NOUN
afs-72443	28	20	serving	serve	VERB
afs-72443	28	21	as	as	ADP
afs-72443	28	22	the	the	DET
afs-72443	28	23	target	target	NOUN
afs-72443	28	24	or	or	CCONJ
afs-72443	28	25	by	by	ADP
afs-72443	28	26	unsuccessful	unsuccessful	ADJ
afs-72443	28	27	amplification	amplification	NOUN
afs-72443	28	28	conditions	condition	NOUN
afs-72443	28	29	.	.	PUNCT
afs-72443	29	1	to	to	PART
afs-72443	29	2	test	test	VERB
afs-72443	29	3	the	the	DET
afs-72443	29	4	amplification	amplification	NOUN
afs-72443	29	5	success	success	NOUN
afs-72443	29	6	,	,	PUNCT
afs-72443	29	7	an	an	DET
afs-72443	29	8	autosomal	autosomal	ADJ
afs-72443	29	9	primer	primer	NOUN
afs-72443	29	10	pair	pair	NOUN
afs-72443	29	11	can	can	AUX
afs-72443	29	12	be	be	AUX
afs-72443	29	13	added	add	VERB
afs-72443	29	14	to	to	ADP
afs-72443	29	15	the	the	DET
afs-72443	29	16	reaction	reaction	NOUN
afs-72443	29	17	mix	mix	NOUN
afs-72443	29	18	.	.	PUNCT
afs-72443	30	1	flowever	flowever	NOUN
afs-72443	30	2	,	,	PUNCT
afs-72443	30	3	this	this	PRON
afs-72443	30	4	usually	usually	ADV
afs-72443	30	5	leads	lead	VERB
afs-72443	30	6	to	to	ADP
afs-72443	30	7	a	a	DET
afs-72443	30	8	decreased	decrease	VERB
afs-72443	30	9	yield	yield	NOUN
afs-72443	30	10	of	of	ADP
afs-72443	30	11	the	the	DET
afs-72443	30	12	male	male	NOUN
afs-72443	30	13	-	-	PUNCT
afs-72443	30	14	specific	specific	ADJ
afs-72443	30	15	product	product	NOUN
afs-72443	30	16	,	,	PUNCT
afs-72443	30	17	sometimes	sometimes	ADV
afs-72443	30	18	at	at	ADP
afs-72443	30	19	levels	level	NOUN
afs-72443	30	20	below	below	ADP
afs-72443	30	21	distinction	distinction	NOUN
afs-72443	30	22	.	.	PUNCT
afs-72443	31	1	usually	usually	ADV
afs-72443	31	2	this	this	PRON
afs-72443	31	3	is	be	AUX
afs-72443	31	4	not	not	PART
afs-72443	31	5	a	a	DET
afs-72443	31	6	problem	problem	NOUN
afs-72443	31	7	when	when	SCONJ
afs-72443	31	8	a	a	DET
afs-72443	31	9	large	large	ADJ
afs-72443	31	10	amount	amount	NOUN
afs-72443	31	11	of	of	ADP
afs-72443	31	12	dna	dna	NOUN
afs-72443	31	13	is	be	AUX
afs-72443	31	14	available	available	ADJ
afs-72443	31	15	as	as	ADP
afs-72443	31	16	template	template	NOUN
afs-72443	31	17	,	,	PUNCT
afs-72443	31	18	but	but	CCONJ
afs-72443	31	19	for	for	ADP
afs-72443	31	20	sexing	sex	VERB
afs-72443	31	21	embryos	embryo	NOUN
afs-72443	31	22	only	only	ADV
afs-72443	31	23	a	a	DET
afs-72443	31	24	few	few	ADJ
afs-72443	31	25	cells	cell	NOUN
afs-72443	31	26	of	of	ADP
afs-72443	31	27	the	the	DET
afs-72443	31	28	embryo	embryo	NOUN
afs-72443	31	29	can	can	AUX
afs-72443	31	30	be	be	AUX
afs-72443	31	31	sacrificed	sacrifice	VERB
afs-72443	31	32	.	.	PUNCT
afs-72443	32	1	thus	thus	ADV
afs-72443	32	2	,	,	PUNCT
afs-72443	32	3	the	the	DET
afs-72443	32	4	testing	testing	NOUN
afs-72443	32	5	of	of	ADP
afs-72443	32	6	the	the	DET
afs-72443	32	7	method	method	NOUN
afs-72443	32	8	should	should	AUX
afs-72443	32	9	be	be	AUX
afs-72443	32	10	done	do	VERB
afs-72443	32	11	with	with	ADP
afs-72443	32	12	a	a	DET
afs-72443	32	13	limited	limited	ADJ
afs-72443	32	14	number	number	NOUN
afs-72443	32	15	of	of	ADP
afs-72443	32	16	cells	cell	NOUN
afs-72443	32	17	,	,	PUNCT
afs-72443	32	18	preferably	preferably	ADV
afs-72443	32	19	with	with	ADP
afs-72443	32	20	embryonic	embryonic	ADJ
afs-72443	32	21	cells	cell	NOUN
afs-72443	32	22	.	.	PUNCT
afs-72443	33	1	this	this	PRON
afs-72443	33	2	introduces	introduce	VERB
afs-72443	33	3	another	another	DET
afs-72443	33	4	problem	problem	NOUN
afs-72443	33	5	,	,	PUNCT
afs-72443	33	6	the	the	DET
afs-72443	33	7	availability	availability	NOUN
afs-72443	33	8	of	of	ADP
afs-72443	33	9	embryonic	embryonic	ADJ
afs-72443	33	10	material	material	NOUN
afs-72443	33	11	for	for	ADP
afs-72443	33	12	optimizing	optimize	VERB
afs-72443	33	13	pcr	pcr	ADJ
afs-72443	33	14	conditions	condition	NOUN
afs-72443	33	15	.	.	PUNCT
afs-72443	34	1	livestock	livestock	NOUN
afs-72443	34	2	embryos	embryo	NOUN
afs-72443	34	3	are	be	AUX
afs-72443	34	4	expensive	expensive	ADJ
afs-72443	34	5	,	,	PUNCT
afs-72443	34	6	and	and	CCONJ
afs-72443	34	7	embryos	embryo	NOUN
afs-72443	34	8	from	from	ADP
afs-72443	34	9	other	other	ADJ
afs-72443	34	10	species	specie	NOUN
afs-72443	34	11	can	can	AUX
afs-72443	34	12	usually	usually	ADV
afs-72443	34	13	not	not	PART
afs-72443	34	14	be	be	AUX
afs-72443	34	15	used	use	VERB
afs-72443	34	16	because	because	SCONJ
afs-72443	34	17	the	the	DET
afs-72443	34	18	male	male	NOUN
afs-72443	34	19	-	-	PUNCT
afs-72443	34	20	specific	specific	ADJ
afs-72443	34	21	primers	primer	NOUN
afs-72443	34	22	tend	tend	VERB
afs-72443	34	23	to	to	PART
afs-72443	34	24	be	be	AUX
afs-72443	34	25	more	more	ADV
afs-72443	34	26	or	or	CCONJ
afs-72443	34	27	less	less	ADJ
afs-72443	34	28	species	specie	NOUN
afs-72443	34	29	-	-	PUNCT
afs-72443	34	30	specific	specific	ADJ
afs-72443	34	31	.	.	PUNCT
afs-72443	35	1	this	this	DET
afs-72443	35	2	paper	paper	NOUN
afs-72443	35	3	presents	present	VERB
afs-72443	35	4	a	a	DET
afs-72443	35	5	pcr	pcr	ADJ
afs-72443	35	6	protocol	protocol	NOUN
afs-72443	35	7	for	for	ADP
afs-72443	35	8	sexing	sex	VERB
afs-72443	35	9	bovine	bovine	NOUN
afs-72443	35	10	and	and	CCONJ
afs-72443	35	11	ovine	ovine	ADJ
afs-72443	35	12	embryos	embryo	NOUN
afs-72443	35	13	without	without	ADP
afs-72443	35	14	the	the	DET
afs-72443	35	15	need	need	NOUN
afs-72443	35	16	for	for	ADP
afs-72443	35	17	autosomal	autosomal	ADJ
afs-72443	35	18	control	control	NOUN
afs-72443	35	19	primers	primer	NOUN
afs-72443	35	20	.	.	PUNCT
afs-72443	36	1	the	the	DET
afs-72443	36	2	amplified	amplify	VERB
afs-72443	36	3	sequence	sequence	NOUN
afs-72443	36	4	is	be	AUX
afs-72443	36	5	within	within	ADP
afs-72443	36	6	the	the	DET
afs-72443	36	7	zfy	zfy	PROPN
afs-72443	36	8	locus	locus	NOUN
afs-72443	36	9	.	.	PUNCT
afs-72443	37	1	this	this	DET
afs-72443	37	2	locus	locus	NOUN
afs-72443	37	3	is	be	AUX
afs-72443	37	4	present	present	ADJ
afs-72443	37	5	on	on	ADP
afs-72443	37	6	the	the	DET
afs-72443	37	7	y	y	PROPN
afs-72443	37	8	chromosome	chromosome	NOUN
afs-72443	37	9	of	of	ADP
afs-72443	37	10	all	all	DET
afs-72443	37	11	placental	placental	ADJ
afs-72443	37	12	species	specie	NOUN
afs-72443	37	13	and	and	CCONJ
afs-72443	37	14	has	have	VERB
afs-72443	37	15	a	a	DET
afs-72443	37	16	homologue	homologue	NOUN
afs-72443	37	17	on	on	ADP
afs-72443	37	18	the	the	DET
afs-72443	37	19	x	x	PUNCT
afs-72443	37	20	chromosome	chromosome	NOUN
afs-72443	37	21	(	(	PUNCT
afs-72443	37	22	page	page	NOUN
afs-72443	37	23	et	et	PROPN
afs-72443	37	24	al	al	PROPN
afs-72443	37	25	.	.	PROPN
afs-72443	37	26	1987	1987	NUM
afs-72443	37	27	)	)	PUNCT
afs-72443	37	28	.	.	PUNCT
afs-72443	38	1	in	in	ADP
afs-72443	38	2	the	the	DET
afs-72443	38	3	mouse	mouse	NOUN
afs-72443	38	4	,	,	PUNCT
afs-72443	38	5	there	there	PRON
afs-72443	38	6	are	be	VERB
afs-72443	38	7	two	two	NUM
afs-72443	38	8	homologues	homologue	NOUN
afs-72443	38	9	on	on	ADP
afs-72443	38	10	the	the	DET
afs-72443	38	11	y	y	NOUN
afs-72443	38	12	-	-	PUNCT
afs-72443	38	13	chromosome	chromosome	NOUN
afs-72443	38	14	,	,	PUNCT
afs-72443	38	15	one	one	NUM
afs-72443	38	16	on	on	ADP
afs-72443	38	17	the	the	DET
afs-72443	38	18	x	x	NOUN
afs-72443	38	19	-	-	NOUN
afs-72443	38	20	chromosome	chromosome	NOUN
afs-72443	38	21	and	and	CCONJ
afs-72443	38	22	a	a	DET
afs-72443	38	23	fourth	fourth	ADJ
afs-72443	38	24	autosomal	autosomal	ADJ
afs-72443	38	25	homologue	homologue	NOUN
afs-72443	38	26	.	.	PUNCT
afs-72443	39	1	male	male	ADJ
afs-72443	39	2	and	and	CCONJ
afs-72443	39	3	female	female	ADJ
afs-72443	39	4	products	product	NOUN
afs-72443	39	5	can	can	AUX
afs-72443	39	6	be	be	AUX
afs-72443	39	7	identified	identify	VERB
afs-72443	39	8	due	due	ADP
afs-72443	39	9	to	to	ADP
afs-72443	39	10	differences	difference	NOUN
afs-72443	39	11	in	in	ADP
afs-72443	39	12	the	the	DET
afs-72443	39	13	restriction	restriction	NOUN
afs-72443	39	14	fragment	fragment	NOUN
afs-72443	39	15	length	length	NOUN
afs-72443	39	16	polymorphism	polymorphism	NOUN
afs-72443	39	17	(	(	PUNCT
afs-72443	39	18	rflp	rflp	PROPN
afs-72443	39	19	)	)	PUNCT
afs-72443	39	20	of	of	ADP
afs-72443	39	21	the	the	DET
afs-72443	39	22	zfy	zfy	PROPN
afs-72443	39	23	/	/	SYM
afs-72443	39	24	zfx	zfx	NOUN
afs-72443	39	25	locus	locus	NOUN
afs-72443	39	26	(	(	PUNCT
afs-72443	39	27	aasen	aasen	NOUN
afs-72443	39	28	and	and	CCONJ
afs-72443	39	29	medrano	medrano	NOUN
afs-72443	39	30	1990	1990	NUM
afs-72443	39	31	)	)	PUNCT
afs-72443	39	32	.	.	PUNCT
afs-72443	40	1	mouse	mouse	NOUN
afs-72443	40	2	embryos	embryo	NOUN
afs-72443	40	3	were	be	AUX
afs-72443	40	4	used	use	VERB
afs-72443	40	5	for	for	ADP
afs-72443	40	6	optimizing	optimize	VERB
afs-72443	40	7	and	and	CCONJ
afs-72443	40	8	testing	test	VERB
afs-72443	40	9	the	the	DET
afs-72443	40	10	protocol	protocol	NOUN
afs-72443	40	11	.	.	PUNCT
afs-72443	41	1	material	material	NOUN
afs-72443	41	2	and	and	CCONJ
afs-72443	41	3	methods	method	NOUN
afs-72443	41	4	the	the	DET
afs-72443	41	5	oligonucleotide	oligonucleotide	ADJ
afs-72443	41	6	primers	primer	NOUN
afs-72443	41	7	were	be	AUX
afs-72443	41	8	modified	modify	VERB
afs-72443	41	9	after	after	ADP
afs-72443	41	10	aasen	aasen	NOUN
afs-72443	41	11	and	and	CCONJ
afs-72443	41	12	medrano	medrano	NOUN
afs-72443	41	13	(	(	PUNCT
afs-72443	41	14	1990	1990	NUM
afs-72443	41	15	)	)	PUNCT
afs-72443	41	16	to	to	PART
afs-72443	41	17	match	match	VERB
afs-72443	41	18	the	the	DET
afs-72443	41	19	mouse	mouse	NOUN
afs-72443	41	20	zfy	zfy	NOUN
afs-72443	41	21	sequence	sequence	NOUN
afs-72443	41	22	as	as	SCONJ
afs-72443	41	23	reported	report	VERB
afs-72443	41	24	by	by	ADP
afs-72443	41	25	ashworth	ashworth	PROPN
afs-72443	41	26	et	et	PROPN
afs-72443	41	27	al	al	PROPN
afs-72443	41	28	.	.	PROPN
afs-72443	42	1	(	(	PUNCT
afs-72443	42	2	1989	1989	NUM
afs-72443	42	3	)	)	PUNCT
afs-72443	42	4	.	.	PUNCT
afs-72443	43	1	the	the	DET
afs-72443	43	2	primer	primer	NOUN
afs-72443	43	3	sequences	sequence	NOUN
afs-72443	43	4	were	be	AUX
afs-72443	43	5	the	the	DET
afs-72443	43	6	following	following	NOUN
afs-72443	43	7	:	:	PUNCT
afs-72443	43	8	pl	pl	NOUN
afs-72443	43	9	-	-	PUNCT
afs-72443	43	10	sez	sez	NOUN
afs-72443	43	11	:	:	PUNCT
afs-72443	43	12	s'-ata	s'-ata	PROPN
afs-72443	43	13	atc	atc	NOUN
afs-72443	43	14	aca	aca	PROPN
afs-72443	43	15	tgg	tgg	PROPN
afs-72443	43	16	aga	aga	PROPN
afs-72443	43	17	gccaca	gccaca	PROPN
afs-72443	43	18	agc	agc	PROPN
afs-72443	43	19	t3	t3	PROPN
afs-72443	43	20	'	'	PUNCT
afs-72443	43	21	p3	p3	PROPN
afs-72443	43	22	-	-	PUNCT
afs-72443	43	23	3mz	3mz	NOUN
afs-72443	43	24	:	:	PUNCT
afs-72443	43	25	s'-gag	s'-gag	PROPN
afs-72443	43	26	cct	cct	PROPN
afs-72443	43	27	ctt	ctt	PROPN
afs-72443	43	28	tgg	tgg	PROPN
afs-72443	43	29	tat	tat	PROPN
afs-72443	43	30	ctg	ctg	PROPN
afs-72443	43	31	aga	aga	PROPN
afs-72443	43	32	aag	aag	PROPN
afs-72443	43	33	t3	t3	PROPN
afs-72443	43	34	'	'	PART
afs-72443	43	35	initial	initial	ADJ
afs-72443	43	36	optimization	optimization	NOUN
afs-72443	43	37	of	of	ADP
afs-72443	43	38	pcr	pcr	ADJ
afs-72443	43	39	parameters	parameter	NOUN
afs-72443	43	40	was	be	AUX
afs-72443	43	41	done	do	VERB
afs-72443	43	42	using	use	VERB
afs-72443	43	43	dna	dna	PROPN
afs-72443	43	44	extracted	extract	VERB
afs-72443	43	45	from	from	ADP
afs-72443	43	46	tails	tail	NOUN
afs-72443	43	47	of	of	ADP
afs-72443	43	48	female	female	ADJ
afs-72443	43	49	and	and	CCONJ
afs-72443	43	50	male	male	ADJ
afs-72443	43	51	mice	mouse	NOUN
afs-72443	43	52	after	after	ADP
afs-72443	43	53	overnight	overnight	ADJ
afs-72443	43	54	incubation	incubation	NOUN
afs-72443	43	55	in	in	ADP
afs-72443	43	56	proteinase	proteinase	PROPN
afs-72443	43	57	k	k	PROPN
afs-72443	43	58	followed	follow	VERB
afs-72443	43	59	by	by	ADP
afs-72443	43	60	phenol	phenol	NOUN
afs-72443	43	61	/	/	SYM
afs-72443	43	62	chlorophorm	chlorophorm	NOUN
afs-72443	43	63	and	and	CCONJ
afs-72443	43	64	ethanol	ethanol	NOUN
afs-72443	43	65	precipitation	precipitation	NOUN
afs-72443	43	66	.	.	PUNCT
afs-72443	44	1	the	the	DET
afs-72443	44	2	sample	sample	NOUN
afs-72443	44	3	amount	amount	NOUN
afs-72443	44	4	was	be	AUX
afs-72443	44	5	approximately	approximately	ADV
afs-72443	44	6	80	80	NUM
afs-72443	44	7	pg	pg	NOUN
afs-72443	44	8	.	.	PUNCT
afs-72443	45	1	further	further	ADJ
afs-72443	45	2	optimization	optimization	NOUN
afs-72443	45	3	was	be	AUX
afs-72443	45	4	carried	carry	VERB
afs-72443	45	5	out	out	ADP
afs-72443	45	6	with	with	ADP
afs-72443	45	7	embryos	embryo	NOUN
afs-72443	45	8	recovered	recover	VERB
afs-72443	45	9	from	from	ADP
afs-72443	45	10	mice	mouse	NOUN
afs-72443	45	11	superovulated	superovulate	VERB
afs-72443	45	12	by	by	ADP
afs-72443	45	13	standard	standard	ADJ
afs-72443	45	14	procedures	procedure	NOUN
afs-72443	45	15	.	.	PUNCT
afs-72443	46	1	fresh	fresh	ADJ
afs-72443	46	2	,	,	PUNCT
afs-72443	46	3	refrigerator	refrigerator	NOUN
afs-72443	46	4	-	-	PUNCT
afs-72443	46	5	stored	store	VERB
afs-72443	46	6	or	or	CCONJ
afs-72443	46	7	frozen	frozen	ADJ
afs-72443	46	8	cattle	cattle	NOUN
afs-72443	46	9	and	and	CCONJ
afs-72443	46	10	sheep	sheep	NOUN
afs-72443	46	11	embryos	embryo	NOUN
afs-72443	46	12	were	be	AUX
afs-72443	46	13	biopsied	biopsie	VERB
afs-72443	46	14	using	use	VERB
afs-72443	46	15	the	the	DET
afs-72443	46	16	'	'	PUNCT
afs-72443	46	17	scratched	scratch	VERB
afs-72443	46	18	-	-	PUNCT
afs-72443	46	19	bottom	bottom	NOUN
afs-72443	46	20	'	'	PUNCT
afs-72443	46	21	technique	technique	NOUN
afs-72443	46	22	(	(	PUNCT
afs-72443	46	23	bredbacka	bredbacka	NOUN
afs-72443	46	24	1991	1991	NUM
afs-72443	46	25	)	)	PUNCT
afs-72443	46	26	.	.	PUNCT
afs-72443	47	1	biopsies	biopsy	NOUN
afs-72443	47	2	were	be	AUX
afs-72443	47	3	transferred	transfer	VERB
afs-72443	47	4	to	to	ADP
afs-72443	47	5	microtubes	microtube	NOUN
afs-72443	47	6	with	with	ADP
afs-72443	47	7	a	a	DET
afs-72443	47	8	micropipette	micropipette	NOUN
afs-72443	47	9	having	have	VERB
afs-72443	47	10	an	an	DET
afs-72443	47	11	inner	inner	ADJ
afs-72443	47	12	diameter	diameter	NOUN
afs-72443	47	13	only	only	ADV
afs-72443	47	14	slightly	slightly	ADV
afs-72443	47	15	larger	large	ADJ
afs-72443	47	16	than	than	ADP
afs-72443	47	17	the	the	DET
afs-72443	47	18	diameter	diameter	NOUN
afs-72443	47	19	ofan	ofan	NOUN
afs-72443	47	20	embryo	embryo	NOUN
afs-72443	47	21	.	.	PUNCT
afs-72443	48	1	in	in	ADP
afs-72443	48	2	some	some	DET
afs-72443	48	3	instances	instance	NOUN
afs-72443	48	4	,	,	PUNCT
afs-72443	48	5	siliconized	siliconize	VERB
afs-72443	48	6	pipettes	pipette	NOUN
afs-72443	48	7	were	be	AUX
afs-72443	48	8	used	use	VERB
afs-72443	48	9	.	.	PUNCT
afs-72443	49	1	up	up	ADP
afs-72443	49	2	to	to	PART
afs-72443	49	3	four	four	NUM
afs-72443	49	4	portions	portion	NOUN
afs-72443	49	5	from	from	ADP
afs-72443	49	6	each	each	DET
afs-72443	49	7	embryo	embryo	NOUN
afs-72443	49	8	were	be	AUX
afs-72443	49	9	separately	separately	ADV
afs-72443	49	10	amplified	amplify	VERB
afs-72443	49	11	and	and	CCONJ
afs-72443	49	12	the	the	DET
afs-72443	49	13	accuracy	accuracy	NOUN
afs-72443	49	14	of	of	ADP
afs-72443	49	15	the	the	DET
afs-72443	49	16	method	method	NOUN
afs-72443	49	17	was	be	AUX
afs-72443	49	18	measured	measure	VERB
afs-72443	49	19	in	in	ADP
afs-72443	49	20	a	a	DET
afs-72443	49	21	blind	blind	ADJ
afs-72443	49	22	test	test	NOUN
afs-72443	49	23	.	.	PUNCT
afs-72443	50	1	prior	prior	ADV
afs-72443	50	2	to	to	ADP
afs-72443	50	3	pcr	pcr	PROPN
afs-72443	50	4	,	,	PUNCT
afs-72443	50	5	the	the	DET
afs-72443	50	6	cells	cell	NOUN
afs-72443	50	7	were	be	AUX
afs-72443	50	8	lysed	lyse	VERB
afs-72443	50	9	either	either	ADV
afs-72443	50	10	by	by	ADP
afs-72443	50	11	1hour	1hour	NUM
afs-72443	50	12	incubation	incubation	NOUN
afs-72443	50	13	withproteinase	withproteinase	NOUN
afs-72443	50	14	k	k	PROPN
afs-72443	50	15	at	at	ADP
afs-72443	50	16	37	37	NUM
afs-72443	50	17	°	°	NOUN
afs-72443	50	18	c	c	NOUN
afs-72443	50	19	followed	follow	VERB
afs-72443	50	20	by	by	ADP
afs-72443	50	21	inactivation	inactivation	NOUN
afs-72443	50	22	at	at	ADP
afs-72443	50	23	99	99	NUM
afs-72443	50	24	°	°	ADP
afs-72443	50	25	c	c	NOUN
afs-72443	50	26	(	(	PUNCT
afs-72443	50	27	8	8	NUM
afs-72443	50	28	minutes	minute	NOUN
afs-72443	50	29	)	)	PUNCT
afs-72443	50	30	,	,	PUNCT
afs-72443	50	31	or	or	CCONJ
afs-72443	50	32	alternatively	alternatively	ADV
afs-72443	50	33	by	by	ADP
afs-72443	50	34	a	a	DET
afs-72443	50	35	modification	modification	NOUN
afs-72443	50	36	of	of	ADP
afs-72443	50	37	the	the	DET
afs-72443	50	38	sperm	sperm	NOUN
afs-72443	50	39	lysis	lysis	NOUN
afs-72443	50	40	described	describe	VERB
afs-72443	50	41	by	by	ADP
afs-72443	50	42	cui	cui	PROPN
afs-72443	50	43	et	et	PROPN
afs-72443	50	44	al	al	PROPN
afs-72443	50	45	(	(	PUNCT
afs-72443	50	46	1989	1989	NUM
afs-72443	50	47	)	)	PUNCT
afs-72443	50	48	.	.	PUNCT
afs-72443	51	1	in	in	ADP
afs-72443	51	2	the	the	DET
afs-72443	51	3	latter	latter	ADJ
afs-72443	51	4	method	method	NOUN
afs-72443	51	5	,	,	PUNCT
afs-72443	51	6	the	the	DET
afs-72443	51	7	sample	sample	NOUN
afs-72443	51	8	,	,	PUNCT
afs-72443	51	9	which	which	PRON
afs-72443	51	10	is	be	AUX
afs-72443	51	11	in	in	ADP
afs-72443	51	12	1	1	NUM
afs-72443	51	13	pi	pi	NOUN
afs-72443	51	14	of	of	ADP
afs-72443	51	15	medium	medium	NOUN
afs-72443	51	16	(	(	PUNCT
afs-72443	51	17	pbs+	pbs+	PROPN
afs-72443	51	18	4	4	NUM
afs-72443	51	19	mg	mg	NUM
afs-72443	51	20	/	/	SYM
afs-72443	51	21	ml	ml	X
afs-72443	51	22	polyvinylpyrrolidone	polyvinylpyrrolidone	NOUN
afs-72443	51	23	)	)	PUNCT
afs-72443	51	24	,	,	PUNCT
afs-72443	51	25	is	be	AUX
afs-72443	51	26	added	add	VERB
afs-72443	51	27	to	to	ADP
afs-72443	51	28	2.5	2.5	NUM
afs-72443	51	29	pi	pi	NOUN
afs-72443	51	30	of	of	ADP
afs-72443	51	31	200	200	NUM
afs-72443	51	32	mm	mm	PROPN
afs-72443	51	33	k.oh/50	k.oh/50	NOUN
afs-72443	51	34	mm	mm	PROPN
afs-72443	51	35	dithiotreitol	dithiotreitol	NOUN
afs-72443	51	36	and	and	CCONJ
afs-72443	51	37	incubated	incubate	VERB
afs-72443	51	38	for	for	ADP
afs-72443	51	39	10	10	NUM
afs-72443	51	40	minutes	minute	NOUN
afs-72443	51	41	at	at	ADP
afs-72443	51	42	65	65	NUM
afs-72443	51	43	°	°	NOUN
afs-72443	51	44	c	c	NOUN
afs-72443	51	45	,	,	PUNCT
afs-72443	51	46	followed	follow	VERB
afs-72443	51	47	by	by	ADP
afs-72443	51	48	neutralization	neutralization	NOUN
afs-72443	51	49	with	with	ADP
afs-72443	51	50	2.5	2.5	NUM
afs-72443	51	51	pi	pi	NOUN
afs-72443	51	52	of	of	ADP
afs-72443	51	53	900	900	NUM
afs-72443	51	54	mm	mm	NUM
afs-72443	51	55	tris	tris	PROPN
afs-72443	51	56	-	-	PUNCT
afs-72443	51	57	hci	hci	NOUN
afs-72443	51	58	(	(	PUNCT
afs-72443	51	59	ph	ph	PROPN
afs-72443	51	60	8.3)/200	8.3)/200	NUM
afs-72443	51	61	mm	mm	PROPN
afs-72443	51	62	hci	hci	NOUN
afs-72443	51	63	.	.	PUNCT
afs-72443	52	1	the	the	DET
afs-72443	52	2	final	final	ADJ
afs-72443	52	3	pcr	pcr	NOUN
afs-72443	52	4	mixture	mixture	NOUN
afs-72443	52	5	contained	contain	VERB
afs-72443	52	6	the	the	DET
afs-72443	52	7	sample	sample	NOUN
afs-72443	52	8	in	in	ADP
afs-72443	52	9	10	10	NUM
afs-72443	52	10	-	-	SYM
afs-72443	52	11	100	100	NUM
afs-72443	52	12	mm	mm	NUM
afs-72443	52	13	tris	tris	PROPN
afs-72443	52	14	-	-	PUNCT
afs-72443	52	15	hci	hci	NOUN
afs-72443	52	16	(	(	PUNCT
afs-72443	52	17	ph	ph	PROPN
afs-72443	52	18	8.8	8.8	NUM
afs-72443	52	19	)	)	PUNCT
afs-72443	52	20	,	,	PUNCT
afs-72443	52	21	1.5	1.5	NUM
afs-72443	52	22	mm	mm	NOUN
afs-72443	52	23	mgcl	mgcl	NOUN
afs-72443	52	24	2	2	NUM
afs-72443	52	25	,	,	PUNCT
afs-72443	52	26	50	50	NUM
afs-72443	52	27	mm	mm	NOUN
afs-72443	52	28	kci	kci	NOUN
afs-72443	52	29	,	,	PUNCT
afs-72443	52	30	0.01	0.01	NUM
afs-72443	52	31	%	%	NOUN
afs-72443	52	32	gelatin	gelatin	NOUN
afs-72443	52	33	,	,	PUNCT
afs-72443	52	34	0.1	0.1	NUM
afs-72443	52	35	%	%	NOUN
afs-72443	52	36	triton	triton	PROPN
afs-72443	52	37	x-100	x-100	PROPN
afs-72443	52	38	,	,	PUNCT
afs-72443	52	39	0.2	0.2	NUM
afs-72443	52	40	mm	mm	NOUN
afs-72443	52	41	dntps	dntps	ADJ
afs-72443	52	42	(	(	PUNCT
afs-72443	52	43	datp	datp	VERB
afs-72443	52	44	,	,	PUNCT
afs-72443	52	45	dttp	dttp	ADJ
afs-72443	52	46	,	,	PUNCT
afs-72443	52	47	dgtp	dgtp	NOUN
afs-72443	52	48	,	,	PUNCT
afs-72443	52	49	dctp	dctp	PROPN
afs-72443	52	50	)	)	PUNCT
afs-72443	52	51	,	,	PUNCT
afs-72443	52	52	1.25	1.25	NUM
afs-72443	52	53	1u	1u	NUM
afs-72443	52	54	taq	taq	NOUN
afs-72443	52	55	polymerase	polymerase	NOUN
afs-72443	52	56	(	(	PUNCT
afs-72443	52	57	promega	promega	NOUN
afs-72443	52	58	)	)	PUNCT
afs-72443	52	59	and	and	CCONJ
afs-72443	52	60	20	20	NUM
afs-72443	52	61	pmol	pmol	NOUN
afs-72443	52	62	of	of	ADP
afs-72443	52	63	each	each	DET
afs-72443	52	64	primer	primer	NOUN
afs-72443	52	65	.	.	PUNCT
afs-72443	53	1	the	the	DET
afs-72443	53	2	reaction	reaction	NOUN
afs-72443	53	3	mix	mix	NOUN
afs-72443	53	4	was	be	AUX
afs-72443	53	5	overlaid	overlay	VERB
afs-72443	53	6	with	with	ADP
afs-72443	53	7	25	25	NUM
afs-72443	53	8	pi	pi	NOUN
afs-72443	53	9	of	of	ADP
afs-72443	53	10	mineral	mineral	NOUN
afs-72443	53	11	oil	oil	NOUN
afs-72443	53	12	(	(	PUNCT
afs-72443	53	13	sigma	sigma	PROPN
afs-72443	53	14	)	)	PUNCT
afs-72443	53	15	.	.	PUNCT
afs-72443	54	1	the	the	DET
afs-72443	54	2	'	'	PUNCT
afs-72443	54	3	hot	hot	ADJ
afs-72443	54	4	start	start	NOUN
afs-72443	54	5	'	'	PUNCT
afs-72443	54	6	technique	technique	NOUN
afs-72443	54	7	was	be	AUX
afs-72443	54	8	used	use	VERB
afs-72443	54	9	to	to	PART
afs-72443	54	10	initiate	initiate	VERB
afs-72443	54	11	the	the	DET
afs-72443	54	12	reaction	reaction	NOUN
afs-72443	54	13	:	:	PUNCT
afs-72443	54	14	during	during	ADP
afs-72443	54	15	the	the	DET
afs-72443	54	16	first	first	ADJ
afs-72443	54	17	denaturation	denaturation	NOUN
afs-72443	54	18	step	step	NOUN
afs-72443	54	19	,	,	PUNCT
afs-72443	54	20	the	the	DET
afs-72443	54	21	dntps	dntps	NOUN
afs-72443	54	22	were	be	AUX
afs-72443	54	23	added	add	VERB
afs-72443	54	24	through	through	ADP
afs-72443	54	25	the	the	DET
afs-72443	54	26	oil	oil	NOUN
afs-72443	54	27	layer	layer	NOUN
afs-72443	54	28	of	of	ADP
afs-72443	54	29	each	each	DET
afs-72443	54	30	tube	tube	NOUN
afs-72443	54	31	.	.	PUNCT
afs-72443	55	1	the	the	DET
afs-72443	55	2	final	final	ADJ
afs-72443	55	3	reaction	reaction	NOUN
afs-72443	55	4	volume	volume	NOUN
afs-72443	55	5	was	be	AUX
afs-72443	55	6	25	25	NUM
afs-72443	55	7	pi	pi	NOUN
afs-72443	55	8	.	.	PUNCT
afs-72443	56	1	the	the	DET
afs-72443	56	2	samples	sample	NOUN
afs-72443	56	3	were	be	AUX
afs-72443	56	4	amplified	amplify	VERB
afs-72443	56	5	at	at	ADP
afs-72443	56	6	the	the	DET
afs-72443	56	7	following	follow	VERB
afs-72443	56	8	temperatures	temperature	NOUN
afs-72443	56	9	:	:	PUNCT
afs-72443	56	10	initial	initial	ADJ
afs-72443	56	11	denaturation	denaturation	NOUN
afs-72443	56	12	at	at	ADP
afs-72443	56	13	94	94	NUM
afs-72443	56	14	°	°	ADP
afs-72443	56	15	c	c	NOUN
afs-72443	56	16	for	for	ADP
afs-72443	56	17	3	3	NUM
afs-72443	56	18	minutes	minute	NOUN
afs-72443	56	19	,	,	PUNCT
afs-72443	56	20	then	then	ADV
afs-72443	56	21	10	10	NUM
afs-72443	56	22	cycles	cycle	NOUN
afs-72443	56	23	of	of	ADP
afs-72443	56	24	1	1	NUM
afs-72443	56	25	minute	minute	NOUN
afs-72443	56	26	at	at	ADP
afs-72443	56	27	94	94	NUM
afs-72443	56	28	°	°	ADP
afs-72443	56	29	c	c	NOUN
afs-72443	56	30	(	(	PUNCT
afs-72443	56	31	denaturation	denaturation	PROPN
afs-72443	56	32	)	)	PUNCT
afs-72443	56	33	,	,	PUNCT
afs-72443	56	34	50	50	NUM
afs-72443	56	35	seconds	second	NOUN
afs-72443	56	36	at	at	ADP
afs-72443	56	37	52	52	NUM
afs-72443	56	38	-	-	SYM
afs-72443	56	39	60	60	NUM
afs-72443	56	40	°	°	ADP
afs-72443	56	41	c	c	NOUN
afs-72443	56	42	(	(	PUNCT
afs-72443	56	43	annealing	anneal	VERB
afs-72443	56	44	)	)	PUNCT
afs-72443	56	45	and	and	CCONJ
afs-72443	56	46	30	30	NUM
afs-72443	56	47	seconds	second	NOUN
afs-72443	56	48	at	at	ADP
afs-72443	56	49	72	72	NUM
afs-72443	56	50	°	°	ADP
afs-72443	56	51	c	c	NOUN
afs-72443	56	52	(	(	PUNCT
afs-72443	56	53	extension	extension	NOUN
afs-72443	56	54	)	)	PUNCT
afs-72443	56	55	.	.	PUNCT
afs-72443	57	1	in	in	ADP
afs-72443	57	2	the	the	DET
afs-72443	57	3	following	follow	VERB
afs-72443	57	4	30	30	NUM
afs-72443	57	5	cycles	cycle	NOUN
afs-72443	57	6	the	the	DET
afs-72443	57	7	extension	extension	NOUN
afs-72443	57	8	step	step	NOUN
afs-72443	57	9	was	be	AUX
afs-72443	57	10	45	45	NUM
afs-72443	57	11	seconds	second	NOUN
afs-72443	57	12	,	,	PUNCT
afs-72443	57	13	and	and	CCONJ
afs-72443	57	14	in	in	ADP
afs-72443	57	15	the	the	DET
afs-72443	57	16	last	last	ADJ
afs-72443	57	17	10	10	NUM
afs-72443	57	18	cycles	cycle	NOUN
afs-72443	57	19	it	it	PRON
afs-72443	57	20	was	be	AUX
afs-72443	57	21	1	1	NUM
afs-72443	57	22	minute	minute	NOUN
afs-72443	57	23	.	.	PUNCT
afs-72443	58	1	in	in	ADP
afs-72443	58	2	the	the	DET
afs-72443	58	3	last	last	ADJ
afs-72443	58	4	cycle	cycle	NOUN
afs-72443	58	5	the	the	DET
afs-72443	58	6	234	234	NUM
afs-72443	58	7	agric	agric	NOUN
afs-72443	58	8	.	.	PUNCT
afs-72443	59	1	sei	sei	ADJ
afs-72443	59	2	.	.	PUNCT
afs-72443	59	3	fin	fin	NOUN
afs-72443	59	4	!	!	PUNCT
afs-72443	59	5	.	.	PUNCT
afs-72443	60	1	1	1	NUM
afs-72443	60	2	(	(	PUNCT
afs-72443	60	3	1992	1992	NUM
afs-72443	60	4	)	)	PUNCT
afs-72443	60	5	samples	sample	NOUN
afs-72443	60	6	were	be	AUX
afs-72443	60	7	held	hold	VERB
afs-72443	60	8	at	at	ADP
afs-72443	60	9	72	72	NUM
afs-72443	60	10	°	°	ADP
afs-72443	60	11	c	c	NOUN
afs-72443	60	12	for	for	ADP
afs-72443	60	13	an	an	DET
afs-72443	60	14	additional	additional	ADJ
afs-72443	60	15	5	5	NUM
afs-72443	60	16	minutes	minute	NOUN
afs-72443	60	17	.	.	PUNCT
afs-72443	61	1	after	after	ADP
afs-72443	61	2	pcr	pcr	ADJ
afs-72443	61	3	amplification	amplification	NOUN
afs-72443	61	4	,	,	PUNCT
afs-72443	61	5	10pi	10pi	NOUN
afs-72443	61	6	from	from	ADP
afs-72443	61	7	each	each	DET
afs-72443	61	8	sample	sample	NOUN
afs-72443	61	9	was	be	AUX
afs-72443	61	10	digested	digest	VERB
afs-72443	61	11	with	with	ADP
afs-72443	61	12	suitable	suitable	ADJ
afs-72443	61	13	restriction	restriction	NOUN
afs-72443	61	14	endonucleases	endonuclease	NOUN
afs-72443	61	15	.	.	PUNCT
afs-72443	62	1	the	the	DET
afs-72443	62	2	zfy	zfy	PROPN
afs-72443	62	3	/	/	SYM
afs-72443	62	4	zfx	zfx	NOUN
afs-72443	62	5	product	product	NOUN
afs-72443	62	6	from	from	ADP
afs-72443	62	7	mice	mouse	NOUN
afs-72443	62	8	was	be	AUX
afs-72443	62	9	digested	digest	VERB
afs-72443	62	10	after	after	ADP
afs-72443	62	11	the	the	DET
afs-72443	62	12	addition	addition	NOUN
afs-72443	62	13	of	of	ADP
afs-72443	62	14	4	4	NUM
afs-72443	62	15	iu	iu	ADV
afs-72443	62	16	of	of	ADP
afs-72443	62	17	haelll	haelll	NOUN
afs-72443	62	18	and	and	CCONJ
afs-72443	62	19	10	10	NUM
afs-72443	62	20	pi	pi	NOUN
afs-72443	62	21	of	of	ADP
afs-72443	62	22	10	10	NUM
afs-72443	62	23	mm	mm	PROPN
afs-72443	62	24	tris	tris	PROPN
afs-72443	62	25	-	-	PUNCT
afs-72443	62	26	hci	hci	NOUN
afs-72443	62	27	(	(	PUNCT
afs-72443	62	28	ph	ph	NOUN
afs-72443	62	29	7.5	7.5	NUM
afs-72443	62	30	)	)	PUNCT
afs-72443	62	31	,	,	PUNCT
afs-72443	62	32	18.5	18.5	NUM
afs-72443	62	33	mm	mm	NOUN
afs-72443	62	34	mgclj	mgclj	NOUN
afs-72443	62	35	and	and	CCONJ
afs-72443	62	36	50	50	NUM
afs-72443	62	37	mm	mm	NOUN
afs-72443	62	38	nacl	nacl	NOUN
afs-72443	62	39	(	(	PUNCT
afs-72443	62	40	total	total	ADJ
afs-72443	62	41	reaction	reaction	NOUN
afs-72443	62	42	volume	volume	NOUN
afs-72443	62	43	20.4	20.4	NUM
afs-72443	62	44	pi	pi	NOUN
afs-72443	62	45	)	)	PUNCT
afs-72443	62	46	.	.	PUNCT
afs-72443	63	1	sheep	sheep	NOUN
afs-72443	63	2	amplification	amplification	NOUN
afs-72443	63	3	products	product	NOUN
afs-72443	63	4	were	be	AUX
afs-72443	63	5	added	add	VERB
afs-72443	63	6	to	to	ADP
afs-72443	63	7	5	5	NUM
afs-72443	63	8	1u	1u	NOUN
afs-72443	63	9	of	of	ADP
afs-72443	63	10	sad	sad	ADJ
afs-72443	63	11	in	in	ADP
afs-72443	63	12	10	10	NUM
afs-72443	63	13	pi	pi	NOUN
afs-72443	63	14	of	of	ADP
afs-72443	63	15	buffer	buffer	NOUN
afs-72443	63	16	a	a	DET
afs-72443	63	17	(	(	PUNCT
afs-72443	63	18	boehringer	boehringer	PROPN
afs-72443	63	19	mannheim	mannheim	PROPN
afs-72443	63	20	)	)	PUNCT
afs-72443	63	21	.	.	PUNCT
afs-72443	64	1	cattle	cattle	NOUN
afs-72443	64	2	pcr	pcr	PROPN
afs-72443	64	3	products	product	NOUN
afs-72443	64	4	were	be	AUX
afs-72443	64	5	incubated	incubate	VERB
afs-72443	64	6	with	with	ADP
afs-72443	64	7	5	5	NUM
afs-72443	64	8	iu	iu	ADP
afs-72443	64	9	pstl	pstl	PROPN
afs-72443	64	10	in	in	ADP
afs-72443	64	11	10	10	NUM
afs-72443	64	12	pi	pi	NOUN
afs-72443	64	13	of	of	ADP
afs-72443	64	14	buffer	buffer	NOUN
afs-72443	64	15	h	h	NOUN
afs-72443	64	16	(	(	PUNCT
afs-72443	64	17	boehringer	boehringer	PROPN
afs-72443	64	18	mannheim	mannheim	PROPN
afs-72443	64	19	)	)	PUNCT
afs-72443	64	20	.	.	PUNCT
afs-72443	65	1	all	all	DET
afs-72443	65	2	digestions	digestion	NOUN
afs-72443	65	3	were	be	AUX
afs-72443	65	4	performed	perform	VERB
afs-72443	65	5	in	in	ADP
afs-72443	65	6	37	37	NUM
afs-72443	65	7	°	°	ADP
afs-72443	65	8	c	c	NOUN
afs-72443	65	9	for	for	ADP
afs-72443	65	10	60	60	NUM
afs-72443	65	11	-	-	SYM
afs-72443	65	12	90	90	NUM
afs-72443	65	13	min	min	NOUN
afs-72443	65	14	,	,	PUNCT
afs-72443	65	15	followed	follow	VERB
afs-72443	65	16	by	by	ADP
afs-72443	65	17	15	15	NUM
afs-72443	65	18	min	min	NOUN
afs-72443	65	19	at	at	ADP
afs-72443	65	20	65	65	NUM
afs-72443	65	21	°	°	ADP
afs-72443	65	22	c	c	NOUN
afs-72443	65	23	to	to	PART
afs-72443	65	24	enhance	enhance	VERB
afs-72443	65	25	the	the	DET
afs-72443	65	26	separation	separation	NOUN
afs-72443	65	27	of	of	ADP
afs-72443	65	28	the	the	DET
afs-72443	65	29	products	product	NOUN
afs-72443	65	30	.	.	PUNCT
afs-72443	66	1	dna	dna	PROPN
afs-72443	66	2	fragments	fragment	NOUN
afs-72443	66	3	were	be	AUX
afs-72443	66	4	separated	separate	VERB
afs-72443	66	5	by	by	ADP
afs-72443	66	6	electrophoresis	electrophoresis	NOUN
afs-72443	66	7	(	(	PUNCT
afs-72443	66	8	3	3	NUM
afs-72443	66	9	%	%	NOUN
afs-72443	66	10	agarose	agarose	NOUN
afs-72443	66	11	stained	stain	VERB
afs-72443	66	12	with	with	ADP
afs-72443	66	13	0.5	0.5	NUM
afs-72443	66	14	pg	pg	NOUN
afs-72443	66	15	/	/	SYM
afs-72443	66	16	ml	ml	X
afs-72443	66	17	ethidium	ethidium	ADJ
afs-72443	66	18	bromide	bromide	NOUN
afs-72443	66	19	)	)	PUNCT
afs-72443	66	20	.	.	PUNCT
afs-72443	67	1	the	the	DET
afs-72443	67	2	fragments	fragment	NOUN
afs-72443	67	3	were	be	AUX
afs-72443	67	4	visualizedand	visualizedand	ADV
afs-72443	67	5	photographed	photograph	VERB
afs-72443	67	6	under	under	ADP
afs-72443	67	7	uv	uv	NOUN
afs-72443	67	8	illumination	illumination	NOUN
afs-72443	67	9	.	.	PUNCT
afs-72443	68	1	in	in	ADP
afs-72443	68	2	the	the	DET
afs-72443	68	3	mouse	mouse	NOUN
afs-72443	68	4	,	,	PUNCT
afs-72443	68	5	haelll	haelll	ADV
afs-72443	68	6	cuts	cut	VERB
afs-72443	68	7	within	within	ADP
afs-72443	68	8	the	the	DET
afs-72443	68	9	zfx	zfx	NOUN
afs-72443	68	10	but	but	CCONJ
afs-72443	68	11	not	not	PART
afs-72443	68	12	zfy	zfy	PROPN
afs-72443	68	13	,	,	PUNCT
afs-72443	68	14	yielding	yield	VERB
afs-72443	68	15	two	two	NUM
afs-72443	68	16	fragments	fragment	NOUN
afs-72443	68	17	from	from	ADP
afs-72443	68	18	females	female	NOUN
afs-72443	68	19	(	(	PUNCT
afs-72443	68	20	approximately	approximately	ADV
afs-72443	68	21	200	200	NUM
afs-72443	68	22	and	and	CCONJ
afs-72443	68	23	250	250	NUM
afs-72443	68	24	bp	bp	NOUN
afs-72443	68	25	)	)	PUNCT
afs-72443	68	26	,	,	PUNCT
afs-72443	68	27	whereas	whereas	SCONJ
afs-72443	68	28	in	in	ADP
afs-72443	68	29	males	male	NOUN
afs-72443	68	30	the	the	DET
afs-72443	68	31	uncut	uncut	ADJ
afs-72443	68	32	zfy	zfy	NOUN
afs-72443	68	33	product	product	NOUN
afs-72443	68	34	results	result	NOUN
afs-72443	68	35	in	in	ADP
afs-72443	68	36	a	a	DET
afs-72443	68	37	third	third	ADJ
afs-72443	68	38	band	band	NOUN
afs-72443	68	39	,	,	PUNCT
afs-72443	68	40	about	about	ADV
afs-72443	68	41	450	450	NUM
afs-72443	68	42	bp	bp	NOUN
afs-72443	68	43	in	in	ADP
afs-72443	68	44	size	size	NOUN
afs-72443	68	45	(	(	PUNCT
afs-72443	68	46	j.f	j.f	PROPN
afs-72443	68	47	.	.	PROPN
afs-72443	68	48	medrano	medrano	PROPN
afs-72443	68	49	,	,	PUNCT
afs-72443	68	50	personal	personal	ADJ
afs-72443	68	51	communication	communication	NOUN
afs-72443	68	52	)	)	PUNCT
afs-72443	68	53	.	.	PUNCT
afs-72443	69	1	in	in	ADP
afs-72443	69	2	the	the	DET
afs-72443	69	3	sheep	sheep	NOUN
afs-72443	69	4	the	the	DET
afs-72443	69	5	zfy	zfy	PROPN
afs-72443	69	6	homologue	homologue	NOUN
afs-72443	69	7	remains	remain	VERB
afs-72443	69	8	uncut	uncut	ADJ
afs-72443	69	9	with	with	ADP
afs-72443	69	10	sad	sad	ADJ
afs-72443	69	11	,	,	PUNCT
afs-72443	69	12	while	while	SCONJ
afs-72443	69	13	the	the	DET
afs-72443	69	14	zfx	zfx	NOUN
afs-72443	69	15	homologue	homologue	NOUN
afs-72443	69	16	is	be	AUX
afs-72443	69	17	digested	digest	VERB
afs-72443	69	18	.	.	PUNCT
afs-72443	70	1	males	male	NOUN
afs-72443	70	2	exhibit	exhibit	VERB
afs-72443	70	3	three	three	NUM
afs-72443	70	4	fragments	fragment	NOUN
afs-72443	70	5	,	,	PUNCT
afs-72443	70	6	sized	size	VERB
afs-72443	70	7	173	173	NUM
afs-72443	70	8	,	,	PUNCT
afs-72443	70	9	272	272	NUM
afs-72443	70	10	and	and	CCONJ
afs-72443	70	11	447	447	NUM
afs-72443	70	12	bp	bp	NOUN
afs-72443	70	13	,	,	PUNCT
afs-72443	70	14	while	while	SCONJ
afs-72443	70	15	females	female	NOUN
afs-72443	70	16	lack	lack	VERB
afs-72443	70	17	the	the	DET
afs-72443	70	18	largest	large	ADJ
afs-72443	70	19	one	one	NOUN
afs-72443	70	20	(	(	PUNCT
afs-72443	70	21	aasen	aasen	NOUN
afs-72443	70	22	and	and	CCONJ
afs-72443	70	23	medrano	medrano	NOUN
afs-72443	70	24	1990	1990	NUM
afs-72443	70	25	)	)	PUNCT
afs-72443	70	26	.	.	PUNCT
afs-72443	71	1	sad	sad	ADJ
afs-72443	71	2	also	also	ADV
afs-72443	71	3	digests	digest	VERB
afs-72443	71	4	mouse	mouse	NOUN
afs-72443	71	5	dna	dna	NOUN
afs-72443	71	6	in	in	ADP
afs-72443	71	7	a	a	DET
afs-72443	71	8	similar	similar	ADJ
afs-72443	71	9	pattern	pattern	NOUN
afs-72443	71	10	.	.	PUNCT
afs-72443	72	1	in	in	ADP
afs-72443	72	2	the	the	DET
afs-72443	72	3	bovine	bovine	NOUN
afs-72443	72	4	,	,	PUNCT
afs-72443	72	5	the	the	DET
afs-72443	72	6	zfy	zfy	NOUN
afs-72443	72	7	product	product	NOUN
afs-72443	72	8	is	be	AUX
afs-72443	72	9	digested	digest	VERB
afs-72443	72	10	but	but	CCONJ
afs-72443	72	11	the	the	DET
afs-72443	72	12	zfx	zfx	NOUN
afs-72443	72	13	homologue	homologue	NOUN
afs-72443	72	14	is	be	AUX
afs-72443	72	15	not	not	PART
afs-72443	72	16	.	.	PUNCT
afs-72443	73	1	consequently	consequently	ADV
afs-72443	73	2	,	,	PUNCT
afs-72443	73	3	males	male	NOUN
afs-72443	73	4	show	show	VERB
afs-72443	73	5	three	three	NUM
afs-72443	73	6	bands	band	NOUN
afs-72443	73	7	(	(	PUNCT
afs-72443	73	8	103	103	NUM
afs-72443	73	9	,	,	PUNCT
afs-72443	73	10	344	344	NUM
afs-72443	73	11	and	and	CCONJ
afs-72443	73	12	445	445	NUM
afs-72443	73	13	bp	bp	NOUN
afs-72443	73	14	)	)	PUNCT
afs-72443	73	15	and	and	CCONJ
afs-72443	73	16	only	only	ADV
afs-72443	73	17	one	one	NUM
afs-72443	73	18	(	(	PUNCT
afs-72443	73	19	445	445	NUM
afs-72443	73	20	bp	bp	NOUN
afs-72443	73	21	)	)	PUNCT
afs-72443	73	22	is	be	AUX
afs-72443	73	23	seen	see	VERB
afs-72443	73	24	in	in	ADP
afs-72443	73	25	females	female	NOUN
afs-72443	73	26	(	(	PUNCT
afs-72443	73	27	aasen	aasen	NOUN
afs-72443	73	28	and	and	CCONJ
afs-72443	73	29	medrano	medrano	NOUN
afs-72443	73	30	1990	1990	NUM
afs-72443	73	31	)	)	PUNCT
afs-72443	73	32	.	.	PUNCT
afs-72443	74	1	all	all	DET
afs-72443	74	2	assays	assay	NOUN
afs-72443	74	3	included	include	VERB
afs-72443	74	4	male	male	ADJ
afs-72443	74	5	and	and	CCONJ
afs-72443	74	6	female	female	ADJ
afs-72443	74	7	dna	dna	PROPN
afs-72443	74	8	sample	sample	NOUN
afs-72443	74	9	controls	control	NOUN
afs-72443	74	10	(	(	PUNCT
afs-72443	74	11	extracted	extract	VERB
afs-72443	74	12	from	from	ADP
afs-72443	74	13	blood	blood	NOUN
afs-72443	74	14	)	)	PUNCT
afs-72443	74	15	and	and	CCONJ
afs-72443	74	16	a	a	DET
afs-72443	74	17	blank	blank	ADJ
afs-72443	74	18	control	control	NOUN
afs-72443	74	19	.	.	PUNCT
afs-72443	75	1	results	result	NOUN
afs-72443	75	2	dna	dna	PROPN
afs-72443	75	3	extracted	extract	VERB
afs-72443	75	4	from	from	ADP
afs-72443	75	5	tails	tail	NOUN
afs-72443	75	6	of	of	ADP
afs-72443	75	7	mice	mouse	NOUN
afs-72443	75	8	was	be	AUX
afs-72443	75	9	successfully	successfully	ADV
afs-72443	75	10	amplified	amplify	VERB
afs-72443	75	11	when	when	SCONJ
afs-72443	75	12	54	54	NUM
afs-72443	75	13	°	°	PRON
afs-72443	75	14	c	c	NOUN
afs-72443	75	15	was	be	AUX
afs-72443	75	16	used	use	VERB
afs-72443	75	17	for	for	ADP
afs-72443	75	18	primer	primer	NOUN
afs-72443	75	19	annealing	annealing	NOUN
afs-72443	75	20	,	,	PUNCT
afs-72443	75	21	but	but	CCONJ
afs-72443	75	22	no	no	DET
afs-72443	75	23	detectable	detectable	ADJ
afs-72443	75	24	amplification	amplification	NOUN
afs-72443	75	25	was	be	AUX
afs-72443	75	26	observed	observe	VERB
afs-72443	75	27	using	use	VERB
afs-72443	75	28	60	60	NUM
afs-72443	75	29	°	°	ADP
afs-72443	75	30	c	c	NOUN
afs-72443	75	31	as	as	ADP
afs-72443	75	32	the	the	DET
afs-72443	75	33	annealing	anneal	VERB
afs-72443	75	34	temperature	temperature	NOUN
afs-72443	75	35	.	.	PUNCT
afs-72443	76	1	likewise	likewise	ADV
afs-72443	76	2	,	,	PUNCT
afs-72443	76	3	dna	dna	PROPN
afs-72443	76	4	from	from	ADP
afs-72443	76	5	mouse	mouse	NOUN
afs-72443	76	6	,	,	PUNCT
afs-72443	76	7	sheep	sheep	NOUN
afs-72443	76	8	or	or	CCONJ
afs-72443	76	9	cattle	cattle	NOUN
afs-72443	76	10	embryos	embryo	NOUN
afs-72443	76	11	yielded	yield	VERB
afs-72443	76	12	a	a	DET
afs-72443	76	13	pcr	pcr	ADJ
afs-72443	76	14	product	product	NOUN
afs-72443	76	15	of	of	ADP
afs-72443	76	16	the	the	DET
afs-72443	76	17	expected	expect	VERB
afs-72443	76	18	size	size	NOUN
afs-72443	76	19	using	use	VERB
afs-72443	76	20	the	the	DET
afs-72443	76	21	lower	low	ADJ
afs-72443	76	22	annealing	anneal	VERB
afs-72443	76	23	temperature	temperature	NOUN
afs-72443	76	24	.	.	PUNCT
afs-72443	77	1	biopsies	biopsy	NOUN
afs-72443	77	2	were	be	AUX
afs-72443	77	3	also	also	ADV
afs-72443	77	4	successfully	successfully	ADV
afs-72443	77	5	amplified	amplify	VERB
afs-72443	77	6	,	,	PUNCT
afs-72443	77	7	often	often	ADV
afs-72443	77	8	even	even	ADV
afs-72443	77	9	when	when	SCONJ
afs-72443	77	10	they	they	PRON
afs-72443	77	11	consisted	consist	VERB
afs-72443	77	12	ofone	ofone	ADJ
afs-72443	77	13	single	single	ADJ
afs-72443	77	14	cell	cell	NOUN
afs-72443	77	15	.	.	PUNCT
afs-72443	78	1	although	although	SCONJ
afs-72443	78	2	successful	successful	ADJ
afs-72443	78	3	amplification	amplification	NOUN
afs-72443	78	4	was	be	AUX
afs-72443	78	5	obtained	obtain	VERB
afs-72443	78	6	using	use	VERB
afs-72443	78	7	52	52	NUM
afs-72443	78	8	°	°	NOUN
afs-72443	78	9	c	c	NOUN
afs-72443	78	10	for	for	ADP
afs-72443	78	11	primer	primer	NOUN
afs-72443	78	12	annealing	annealing	NOUN
afs-72443	78	13	,	,	PUNCT
afs-72443	78	14	54	54	NUM
afs-72443	78	15	°	°	NUM
afs-72443	78	16	c	c	NOUN
afs-72443	78	17	appeared	appear	VERB
afs-72443	78	18	to	to	PART
afs-72443	78	19	give	give	VERB
afs-72443	78	20	a	a	DET
afs-72443	78	21	better	well	ADJ
afs-72443	78	22	amplification	amplification	NOUN
afs-72443	78	23	yield	yield	NOUN
afs-72443	78	24	.	.	PUNCT
afs-72443	79	1	enzyme	enzyme	NOUN
afs-72443	79	2	digestion	digestion	NOUN
afs-72443	79	3	of	of	ADP
afs-72443	79	4	the	the	DET
afs-72443	79	5	pcr	pcr	ADJ
afs-72443	79	6	products	product	NOUN
afs-72443	79	7	yielded	yield	VERB
afs-72443	79	8	fragments	fragment	NOUN
afs-72443	79	9	of	of	ADP
afs-72443	79	10	the	the	DET
afs-72443	79	11	expected	expect	VERB
afs-72443	79	12	size	size	NOUN
afs-72443	79	13	(	(	PUNCT
afs-72443	79	14	fig	fig	NOUN
afs-72443	79	15	.	.	NOUN
afs-72443	79	16	1	1	NUM
afs-72443	79	17	and	and	CCONJ
afs-72443	79	18	2	2	NUM
afs-72443	79	19	)	)	PUNCT
afs-72443	79	20	.	.	PUNCT
afs-72443	80	1	in	in	ADP
afs-72443	80	2	the	the	DET
afs-72443	80	3	sheep	sheep	NOUN
afs-72443	80	4	,	,	PUNCT
afs-72443	80	5	a	a	DET
afs-72443	80	6	few	few	ADJ
afs-72443	80	7	of	of	ADP
afs-72443	80	8	the	the	DET
afs-72443	80	9	female	female	ADJ
afs-72443	80	10	samples	sample	NOUN
afs-72443	80	11	showed	show	VERB
afs-72443	80	12	a	a	DET
afs-72443	80	13	weak	weak	ADJ
afs-72443	80	14	band	band	NOUN
afs-72443	80	15	sized	size	VERB
afs-72443	80	16	approximately	approximately	ADV
afs-72443	80	17	450	450	NUM
afs-72443	80	18	bp	bp	NOUN
afs-72443	80	19	.	.	PUNCT
afs-72443	81	1	this	this	PRON
afs-72443	81	2	was	be	AUX
afs-72443	81	3	due	due	ADJ
afs-72443	81	4	to	to	ADP
afs-72443	81	5	incomplete	incomplete	ADJ
afs-72443	81	6	digestion	digestion	NOUN
afs-72443	81	7	,	,	PUNCT
afs-72443	81	8	which	which	PRON
afs-72443	81	9	could	could	AUX
afs-72443	81	10	be	be	AUX
afs-72443	81	11	confirmed	confirm	VERB
afs-72443	81	12	after	after	SCONJ
afs-72443	81	13	repeated	repeat	VERB
afs-72443	81	14	enzyme	enzyme	NOUN
afs-72443	81	15	incubationof	incubationof	VERB
afs-72443	81	16	such	such	ADJ
afs-72443	81	17	samples	sample	NOUN
afs-72443	81	18	.	.	PUNCT
afs-72443	82	1	in	in	ADP
afs-72443	82	2	bovine	bovine	ADJ
afs-72443	82	3	males	male	NOUN
afs-72443	82	4	,	,	PUNCT
afs-72443	82	5	the	the	DET
afs-72443	82	6	103	103	NUM
afs-72443	82	7	bp	bp	PROPN
afs-72443	82	8	fragment	fragment	NOUN
afs-72443	82	9	was	be	AUX
afs-72443	82	10	weaker	weak	ADJ
afs-72443	82	11	than	than	ADP
afs-72443	82	12	the	the	DET
afs-72443	82	13	two	two	NUM
afs-72443	82	14	longer	long	ADJ
afs-72443	82	15	fragments	fragment	NOUN
afs-72443	82	16	,	,	PUNCT
afs-72443	82	17	as	as	SCONJ
afs-72443	82	18	could	could	AUX
afs-72443	82	19	be	be	AUX
afs-72443	82	20	expected	expect	VERB
afs-72443	82	21	because	because	SCONJ
afs-72443	82	22	of	of	ADP
afs-72443	82	23	the	the	DET
afs-72443	82	24	shorter	short	ADJ
afs-72443	82	25	length	length	NOUN
afs-72443	82	26	.	.	PUNCT
afs-72443	83	1	thus	thus	ADV
afs-72443	83	2	the	the	DET
afs-72443	83	3	appearance	appearance	NOUN
afs-72443	83	4	of	of	ADP
afs-72443	83	5	a	a	DET
afs-72443	83	6	344	344	NUM
afs-72443	83	7	bp	bp	PROPN
afs-72443	83	8	fragment	fragment	NOUN
afs-72443	83	9	was	be	AUX
afs-72443	83	10	the	the	DET
afs-72443	83	11	clearest	clear	ADJ
afs-72443	83	12	indication	indication	NOUN
afs-72443	83	13	of	of	ADP
afs-72443	83	14	maleness	maleness	NOUN
afs-72443	83	15	.	.	PUNCT
afs-72443	84	1	in	in	ADP
afs-72443	84	2	most	most	ADJ
afs-72443	84	3	cases	case	NOUN
afs-72443	84	4	it	it	PRON
afs-72443	84	5	was	be	AUX
afs-72443	84	6	possible	possible	ADJ
afs-72443	84	7	to	to	PART
afs-72443	84	8	diagnose	diagnose	VERB
afs-72443	84	9	the	the	DET
afs-72443	84	10	sex	sex	NOUN
afs-72443	84	11	from	from	ADP
afs-72443	84	12	samples	sample	NOUN
afs-72443	84	13	as	as	ADV
afs-72443	84	14	small	small	ADJ
afs-72443	84	15	as	as	ADP
afs-72443	84	16	1	1	NUM
afs-72443	84	17	-	-	SYM
afs-72443	84	18	4	4	NUM
afs-72443	84	19	cells	cell	NOUN
afs-72443	84	20	,	,	PUNCT
afs-72443	84	21	although	although	SCONJ
afs-72443	84	22	the	the	DET
afs-72443	84	23	signals	signal	NOUN
afs-72443	84	24	in	in	ADP
afs-72443	84	25	such	such	ADJ
afs-72443	84	26	samples	sample	NOUN
afs-72443	84	27	were	be	AUX
afs-72443	84	28	usually	usually	ADV
afs-72443	84	29	weaker	weak	ADJ
afs-72443	84	30	than	than	ADP
afs-72443	84	31	those	those	PRON
afs-72443	84	32	obtained	obtain	VERB
afs-72443	84	33	from	from	ADP
afs-72443	84	34	half	half	DET
afs-72443	84	35	or	or	CCONJ
afs-72443	84	36	quarter	quarter	NOUN
afs-72443	84	37	embryos	embryo	NOUN
afs-72443	84	38	.	.	PUNCT
afs-72443	85	1	accuracy	accuracy	NOUN
afs-72443	85	2	testing	testing	NOUN
afs-72443	85	3	was	be	AUX
afs-72443	85	4	done	do	VERB
afs-72443	85	5	with	with	ADP
afs-72443	85	6	sheep	sheep	NOUN
afs-72443	85	7	embryos	embryo	VERB
afs-72443	85	8	,	,	PUNCT
afs-72443	85	9	most	most	ADJ
afs-72443	85	10	of	of	ADP
afs-72443	85	11	which	which	PRON
afs-72443	85	12	were	be	AUX
afs-72443	85	13	of	of	ADP
afs-72443	85	14	poor	poor	ADJ
afs-72443	85	15	quality	quality	NOUN
afs-72443	85	16	.	.	PUNCT
afs-72443	86	1	when	when	SCONJ
afs-72443	86	2	the	the	DET
afs-72443	86	3	embryo	embryo	NOUN
afs-72443	86	4	manipulation	manipulation	NOUN
afs-72443	86	5	and	and	CCONJ
afs-72443	86	6	the	the	DET
afs-72443	86	7	lysis	lysis	NOUN
afs-72443	86	8	of	of	ADP
afs-72443	86	9	samples	sample	NOUN
afs-72443	86	10	were	be	AUX
afs-72443	86	11	done	do	VERB
afs-72443	86	12	under	under	ADP
afs-72443	86	13	farm	farm	NOUN
afs-72443	86	14	conditions	condition	NOUN
afs-72443	86	15	,	,	PUNCT
afs-72443	86	16	21	21	NUM
afs-72443	86	17	replicated	replicate	VERB
afs-72443	86	18	samples	sample	NOUN
afs-72443	86	19	were	be	AUX
afs-72443	86	20	successfully	successfully	ADV
afs-72443	86	21	amplified	amplify	VERB
afs-72443	86	22	from	from	ADP
afs-72443	86	23	14	14	NUM
afs-72443	86	24	embryos	embryo	NOUN
afs-72443	86	25	after	after	ADP
afs-72443	86	26	splitting	split	VERB
afs-72443	86	27	into	into	ADP
afs-72443	86	28	2	2	NUM
afs-72443	86	29	-	-	SYM
afs-72443	86	30	4	4	NUM
afs-72443	86	31	portions	portion	NOUN
afs-72443	86	32	.	.	PUNCT
afs-72443	87	1	no	no	DET
afs-72443	87	2	replicates	replicate	NOUN
afs-72443	87	3	were	be	AUX
afs-72443	87	4	obtained	obtain	VERB
afs-72443	87	5	from	from	ADP
afs-72443	87	6	8	8	NUM
afs-72443	87	7	embryos	embryo	NOUN
afs-72443	87	8	.	.	PUNCT
afs-72443	88	1	of	of	ADP
afs-72443	88	2	all	all	DET
afs-72443	88	3	the	the	DET
afs-72443	88	4	samples	sample	NOUN
afs-72443	88	5	,	,	PUNCT
afs-72443	88	6	67	67	NUM
afs-72443	88	7	%	%	NOUN
afs-72443	88	8	(	(	PUNCT
afs-72443	88	9	41/61	41/61	NUM
afs-72443	88	10	)	)	PUNCT
afs-72443	88	11	could	could	AUX
afs-72443	88	12	be	be	AUX
afs-72443	88	13	sexed	sex	VERB
afs-72443	88	14	.	.	PUNCT
afs-72443	89	1	all	all	DET
afs-72443	89	2	replicates	replicate	NOUN
afs-72443	89	3	from	from	ADP
afs-72443	89	4	a	a	DET
afs-72443	89	5	given	give	VERB
afs-72443	89	6	embryo	embryo	NOUN
afs-72443	89	7	yielded	yield	VERB
afs-72443	89	8	identicalbanding	identicalbande	VERB
afs-72443	89	9	patterns	pattern	NOUN
afs-72443	89	10	.	.	PUNCT
afs-72443	90	1	when	when	SCONJ
afs-72443	90	2	all	all	DET
afs-72443	90	3	procedures	procedure	NOUN
afs-72443	90	4	were	be	AUX
afs-72443	90	5	done	do	VERB
afs-72443	90	6	in	in	ADP
afs-72443	90	7	the	the	DET
afs-72443	90	8	laboratory	laboratory	NOUN
afs-72443	90	9	,	,	PUNCT
afs-72443	90	10	total	total	ADJ
afs-72443	90	11	amplification	amplification	NOUN
afs-72443	90	12	success	success	NOUN
afs-72443	90	13	was	be	AUX
afs-72443	90	14	68	68	NUM
afs-72443	90	15	%	%	NOUN
afs-72443	90	16	(	(	PUNCT
afs-72443	90	17	28/41	28/41	NUM
afs-72443	90	18	)	)	PUNCT
afs-72443	90	19	when	when	SCONJ
afs-72443	90	20	the	the	DET
afs-72443	90	21	samples	sample	NOUN
afs-72443	90	22	were	be	AUX
afs-72443	90	23	lysed	lyse	VERB
afs-72443	90	24	in	in	ADP
afs-72443	90	25	proteinase	proteinase	PROPN
afs-72443	90	26	k.	k.	PROPN
afs-72443	90	27	with	with	ADP
afs-72443	90	28	alkalic	alkalic	ADJ
afs-72443	90	29	treatment	treatment	NOUN
afs-72443	90	30	the	the	DET
afs-72443	90	31	success	success	NOUN
afs-72443	90	32	rate	rate	NOUN
afs-72443	90	33	was	be	AUX
afs-72443	90	34	only	only	ADV
afs-72443	90	35	32	32	NUM
afs-72443	90	36	%	%	NOUN
afs-72443	90	37	(	(	PUNCT
afs-72443	90	38	7/22	7/22	PROPN
afs-72443	90	39	)	)	PUNCT
afs-72443	90	40	.	.	PUNCT
afs-72443	91	1	one	one	NUM
afs-72443	91	2	misidentification	misidentification	NOUN
afs-72443	91	3	of	of	ADP
afs-72443	91	4	15	15	NUM
afs-72443	91	5	replicates	replicate	NOUN
afs-72443	91	6	from	from	ADP
afs-72443	91	7	9	9	NUM
afs-72443	91	8	embryos	embryo	NOUN
afs-72443	91	9	was	be	AUX
afs-72443	91	10	observed	observe	VERB
afs-72443	91	11	.	.	PUNCT
afs-72443	92	1	this	this	DET
afs-72443	92	2	contamination	contamination	NOUN
afs-72443	92	3	was	be	AUX
afs-72443	92	4	most	most	ADV
afs-72443	92	5	likely	likely	ADJ
afs-72443	92	6	due	due	ADP
afs-72443	92	7	to	to	ADP
afs-72443	92	8	the	the	DET
afs-72443	92	9	use	use	NOUN
afs-72443	92	10	of	of	ADP
afs-72443	92	11	the	the	DET
afs-72443	92	12	same	same	ADJ
afs-72443	92	13	micropipette	micropipette	NOUN
afs-72443	92	14	for	for	ADP
afs-72443	92	15	handling	handle	VERB
afs-72443	92	16	all	all	DET
afs-72443	92	17	the	the	DET
afs-72443	92	18	samples	sample	NOUN
afs-72443	92	19	in	in	ADP
afs-72443	92	20	the	the	DET
afs-72443	92	21	laboratory	laboratory	NOUN
afs-72443	92	22	.	.	PUNCT
afs-72443	93	1	on	on	ADP
afs-72443	93	2	the	the	DET
afs-72443	93	3	farm	farm	NOUN
afs-72443	93	4	,	,	PUNCT
afs-72443	93	5	a	a	DET
afs-72443	93	6	separate	separate	ADJ
afs-72443	93	7	micropipette	micropipette	NOUN
afs-72443	93	8	was	be	AUX
afs-72443	93	9	used	use	VERB
afs-72443	93	10	for	for	ADP
afs-72443	93	11	each	each	DET
afs-72443	93	12	sample	sample	NOUN
afs-72443	93	13	.	.	PUNCT
afs-72443	94	1	efficiency	efficiency	NOUN
afs-72443	94	2	(	(	PUNCT
afs-72443	94	3	proportion	proportion	NOUN
afs-72443	94	4	of	of	ADP
afs-72443	94	5	diagnosable	diagnosable	ADJ
afs-72443	94	6	samples	sample	NOUN
afs-72443	94	7	)	)	PUNCT
afs-72443	94	8	depended	depend	VERB
afs-72443	94	9	on	on	ADP
afs-72443	94	10	factors	factor	NOUN
afs-72443	94	11	such	such	ADJ
afs-72443	94	12	as	as	ADP
afs-72443	94	13	biopsy	biopsy	NOUN
afs-72443	94	14	size	size	NOUN
afs-72443	94	15	,	,	PUNCT
afs-72443	94	16	use	use	NOUN
afs-72443	94	17	of	of	ADP
afs-72443	94	18	siliconized	siliconize	VERB
afs-72443	94	19	pipettes	pipette	NOUN
afs-72443	94	20	,	,	PUNCT
afs-72443	94	21	etc	etc	X
afs-72443	94	22	.	.	X
afs-72443	94	23	larger	large	ADJ
afs-72443	94	24	biopsies	biopsy	NOUN
afs-72443	94	25	were	be	AUX
afs-72443	94	26	more	more	ADV
afs-72443	94	27	likely	likely	ADJ
afs-72443	94	28	to	to	PART
afs-72443	94	29	yield	yield	VERB
afs-72443	94	30	detectable	detectable	ADJ
afs-72443	94	31	amounts	amount	NOUN
afs-72443	94	32	of	of	ADP
afs-72443	94	33	the	the	DET
afs-72443	94	34	amplified	amplify	VERB
afs-72443	94	35	product	product	NOUN
afs-72443	94	36	.	.	PUNCT
afs-72443	95	1	the	the	DET
afs-72443	95	2	use	use	NOUN
afs-72443	95	3	ofsiliconized	ofsiliconize	VERB
afs-72443	95	4	pipettes	pipette	NOUN
afs-72443	95	5	appeared	appear	VERB
afs-72443	95	6	to	to	PART
afs-72443	95	7	decrease	decrease	VERB
afs-72443	95	8	the	the	DET
afs-72443	95	9	attachment	attachment	NOUN
afs-72443	95	10	of	of	ADP
afs-72443	95	11	embryos	embryo	NOUN
afs-72443	95	12	to	to	ADP
afs-72443	95	13	the	the	DET
afs-72443	95	14	pipette	pipette	NOUN
afs-72443	95	15	,	,	PUNCT
afs-72443	95	16	thereby	thereby	ADV
afs-72443	95	17	improving	improve	VERB
afs-72443	95	18	the	the	DET
afs-72443	95	19	efficiency	efficiency	NOUN
afs-72443	95	20	.	.	PUNCT
afs-72443	96	1	235	235	NUM
afs-72443	96	2	agric	agric	NOUN
afs-72443	96	3	.	.	PUNCT
afs-72443	97	1	sei	sei	ADJ
afs-72443	97	2	.	.	PUNCT
afs-72443	97	3	finl	finl	NOUN
afs-72443	97	4	.	.	PUNCT
afs-72443	98	1	1	1	NUM
afs-72443	98	2	(	(	PUNCT
afs-72443	98	3	1992	1992	NUM
afs-72443	98	4	)	)	PUNCT
afs-72443	98	5	discussion	discussion	NOUN
afs-72443	98	6	there	there	PRON
afs-72443	98	7	are	be	VERB
afs-72443	98	8	some	some	DET
afs-72443	98	9	reports	report	NOUN
afs-72443	98	10	describing	describe	VERB
afs-72443	98	11	livestock	livestock	NOUN
afs-72443	98	12	embryo	embryo	NOUN
afs-72443	98	13	sexing	sex	VERB
afs-72443	98	14	based	base	VERB
afs-72443	98	15	on	on	ADP
afs-72443	98	16	pcr	pcr	PROPN
afs-72443	98	17	(	(	PUNCT
afs-72443	98	18	herr	herr	PROPN
afs-72443	98	19	et	et	PROPN
afs-72443	98	20	al	al	PROPN
afs-72443	98	21	.	.	PROPN
afs-72443	98	22	1990	1990	NUM
afs-72443	98	23	a	a	DET
afs-72443	98	24	,	,	PUNCT
afs-72443	98	25	b	b	NOUN
afs-72443	98	26	,	,	PUNCT
afs-72443	98	27	schröder	schröder	NOUN
afs-72443	98	28	et	et	PROPN
afs-72443	98	29	al	al	PROPN
afs-72443	98	30	.	.	PROPN
afs-72443	98	31	1990	1990	NUM
afs-72443	98	32	,	,	PUNCT
afs-72443	98	33	bredbacka	bredbacka	PROPN
afs-72443	98	34	et	et	PROPN
afs-72443	98	35	al	al	PROPN
afs-72443	98	36	.	.	PROPN
afs-72443	98	37	1991	1991	NUM
afs-72443	98	38	,	,	PUNCT
afs-72443	98	39	peura	peura	PROPN
afs-72443	98	40	et	et	PROPN
afs-72443	98	41	al	al	PROPN
afs-72443	98	42	.	.	PROPN
afs-72443	98	43	1991	1991	NUM
afs-72443	98	44	,	,	PUNCT
afs-72443	98	45	setiabudi	setiabudi	NOUN
afs-72443	98	46	and	and	CCONJ
afs-72443	98	47	gustafsson	gustafsson	PROPN
afs-72443	98	48	1991	1991	NUM
afs-72443	98	49	)	)	PUNCT
afs-72443	98	50	,	,	PUNCT
afs-72443	98	51	but	but	CCONJ
afs-72443	98	52	detailed	detailed	ADJ
afs-72443	98	53	information	information	NOUN
afs-72443	98	54	is	be	AUX
afs-72443	98	55	often	often	ADV
afs-72443	98	56	lacking	lack	VERB
afs-72443	98	57	.	.	PUNCT
afs-72443	99	1	this	this	PRON
afs-72443	99	2	is	be	AUX
afs-72443	99	3	no	no	ADV
afs-72443	99	4	doubt	doubt	ADV
afs-72443	99	5	partly	partly	ADV
afs-72443	99	6	due	due	ADJ
afs-72443	99	7	to	to	ADP
afs-72443	99	8	the	the	DET
afs-72443	99	9	commercial	commercial	ADJ
afs-72443	99	10	potential	potential	NOUN
afs-72443	99	11	of	of	ADP
afs-72443	99	12	sexing	sex	VERB
afs-72443	99	13	.	.	PUNCT
afs-72443	100	1	in	in	ADP
afs-72443	100	2	this	this	DET
afs-72443	100	3	paper	paper	NOUN
afs-72443	100	4	a	a	DET
afs-72443	100	5	thorough	thorough	ADJ
afs-72443	100	6	protocol	protocol	NOUN
afs-72443	100	7	is	be	AUX
afs-72443	100	8	outlined	outline	VERB
afs-72443	100	9	for	for	ADP
afs-72443	100	10	sexing	sex	VERB
afs-72443	100	11	bovine	bovine	NOUN
afs-72443	100	12	and	and	CCONJ
afs-72443	100	13	ovine	ovine	ADJ
afs-72443	100	14	embryos	embryos	NOUN
afs-72443	100	15	.	.	PUNCT
afs-72443	101	1	although	although	SCONJ
afs-72443	101	2	sheep	sheep	NOUN
afs-72443	101	3	embryo	embryo	NOUN
afs-72443	101	4	sexing	sexing	NOUN
afs-72443	101	5	results	result	NOUN
afs-72443	101	6	have	have	AUX
afs-72443	101	7	been	be	AUX
afs-72443	101	8	published	publish	VERB
afs-72443	101	9	previously	previously	ADV
afs-72443	101	10	(	(	PUNCT
afs-72443	101	11	herr	herr	PROPN
afs-72443	101	12	et	et	PROPN
afs-72443	101	13	al	al	PROPN
afs-72443	101	14	.	.	PROPN
afs-72443	102	1	1990	1990	NUM
afs-72443	102	2	b	b	X
afs-72443	102	3	)	)	PUNCT
afs-72443	102	4	,	,	PUNCT
afs-72443	102	5	this	this	DET
afs-72443	102	6	paper	paper	NOUN
afs-72443	102	7	is	be	AUX
afs-72443	102	8	to	to	ADP
afs-72443	102	9	the	the	DET
afs-72443	102	10	authors	author	NOUN
afs-72443	102	11	'	'	PART
afs-72443	102	12	knowledge	knowledge	NOUN
afs-72443	102	13	the	the	DET
afs-72443	102	14	first	first	ADJ
afs-72443	102	15	to	to	PART
afs-72443	102	16	describe	describe	VERB
afs-72443	102	17	in	in	ADP
afs-72443	102	18	detail	detail	NOUN
afs-72443	102	19	pcr	pcr	NOUN
afs-72443	102	20	-	-	PUNCT
afs-72443	102	21	based	base	VERB
afs-72443	102	22	sexing	sexing	NOUN
afs-72443	102	23	of	of	ADP
afs-72443	102	24	embryonic	embryonic	ADJ
afs-72443	102	25	cells	cell	NOUN
afs-72443	102	26	in	in	ADP
afs-72443	102	27	this	this	DET
afs-72443	102	28	species	specie	NOUN
afs-72443	102	29	.	.	PUNCT
afs-72443	103	1	with	with	ADP
afs-72443	103	2	the	the	DET
afs-72443	103	3	protocol	protocol	NOUN
afs-72443	103	4	presented	present	VERB
afs-72443	103	5	in	in	ADP
afs-72443	103	6	this	this	DET
afs-72443	103	7	paper	paper	NOUN
afs-72443	103	8	,	,	PUNCT
afs-72443	103	9	successful	successful	ADJ
afs-72443	103	10	amplification	amplification	NOUN
afs-72443	103	11	could	could	AUX
afs-72443	103	12	be	be	AUX
afs-72443	103	13	obtained	obtain	VERB
afs-72443	103	14	from	from	ADP
afs-72443	103	15	as	as	ADV
afs-72443	103	16	few	few	ADJ
afs-72443	103	17	as	as	ADP
afs-72443	103	18	1	1	NUM
afs-72443	103	19	-	-	SYM
afs-72443	103	20	4	4	NUM
afs-72443	103	21	blastomeres	blastomere	NOUN
afs-72443	103	22	.	.	PUNCT
afs-72443	104	1	this	this	PRON
afs-72443	104	2	makes	make	VERB
afs-72443	104	3	it	it	PRON
afs-72443	104	4	possible	possible	ADJ
afs-72443	104	5	to	to	PART
afs-72443	104	6	transfer	transfer	VERB
afs-72443	104	7	sexed	sex	VERB
afs-72443	104	8	embryos	embryo	NOUN
afs-72443	104	9	from	from	ADP
afs-72443	104	10	which	which	PRON
afs-72443	104	11	only	only	ADV
afs-72443	104	12	small	small	ADJ
afs-72443	104	13	biopsies	biopsy	NOUN
afs-72443	104	14	have	have	AUX
afs-72443	104	15	been	be	AUX
afs-72443	104	16	removed	remove	VERB
afs-72443	104	17	in	in	ADP
afs-72443	104	18	order	order	NOUN
afs-72443	104	19	to	to	PART
afs-72443	104	20	maintain	maintain	VERB
afs-72443	104	21	high	high	ADJ
afs-72443	104	22	post	post	ADJ
afs-72443	104	23	-	-	ADJ
afs-72443	104	24	transfer	transfer	NOUN
afs-72443	104	25	viability	viability	NOUN
afs-72443	104	26	.	.	PUNCT
afs-72443	105	1	in	in	ADP
afs-72443	105	2	a	a	DET
afs-72443	105	3	recent	recent	ADJ
afs-72443	105	4	study	study	NOUN
afs-72443	105	5	(	(	PUNCT
afs-72443	105	6	peippo	peippo	PROPN
afs-72443	105	7	et	et	PROPN
afs-72443	105	8	al	al	PROPN
afs-72443	105	9	.	.	PROPN
afs-72443	105	10	,	,	PUNCT
afs-72443	105	11	in	in	ADP
afs-72443	105	12	preparation	preparation	NOUN
afs-72443	105	13	)	)	PUNCT
afs-72443	105	14	11	11	NUM
afs-72443	105	15	of	of	ADP
afs-72443	105	16	13	13	NUM
afs-72443	105	17	bovine	bovine	NOUN
afs-72443	105	18	samples	sample	NOUN
afs-72443	105	19	(	(	PUNCT
afs-72443	105	20	85	85	NUM
afs-72443	105	21	%	%	NOUN
afs-72443	105	22	)	)	PUNCT
afs-72443	105	23	could	could	AUX
afs-72443	105	24	be	be	AUX
afs-72443	105	25	sexed	sex	VERB
afs-72443	105	26	with	with	ADP
afs-72443	105	27	the	the	DET
afs-72443	105	28	present	present	ADJ
afs-72443	105	29	method	method	NOUN
afs-72443	105	30	when	when	SCONJ
afs-72443	105	31	the	the	DET
afs-72443	105	32	biopsy	biopsy	NOUN
afs-72443	105	33	size	size	NOUN
afs-72443	105	34	was	be	AUX
afs-72443	105	35	about	about	ADV
afs-72443	105	36	25	25	NUM
afs-72443	105	37	%	%	NOUN
afs-72443	105	38	of	of	ADP
afs-72443	105	39	a	a	DET
afs-72443	105	40	morula	morula	NOUN
afs-72443	105	41	stage	stage	NOUN
afs-72443	105	42	embryo	embryo	NOUN
afs-72443	105	43	.	.	PUNCT
afs-72443	106	1	in	in	ADP
afs-72443	106	2	the	the	DET
afs-72443	106	3	same	same	ADJ
afs-72443	106	4	study	study	NOUN
afs-72443	106	5	,	,	PUNCT
afs-72443	106	6	sex	sex	NOUN
afs-72443	106	7	was	be	AUX
afs-72443	106	8	confirmed	confirm	VERB
afs-72443	106	9	using	use	VERB
afs-72443	106	10	another	another	DET
afs-72443	106	11	pcr	pcr	NOUN
afs-72443	106	12	-	-	PUNCT
afs-72443	106	13	based	base	VERB
afs-72443	106	14	sexing	sexing	NOUN
afs-72443	106	15	method	method	NOUN
afs-72443	106	16	with	with	ADP
afs-72443	106	17	all	all	DET
afs-72443	106	18	embryos	embryo	NOUN
afs-72443	106	19	(	(	PUNCT
afs-72443	106	20	10/10	10/10	NUM
afs-72443	106	21	)	)	PUNCT
afs-72443	106	22	yielding	yield	VERB
afs-72443	106	23	identical	identical	ADJ
afs-72443	106	24	results	result	NOUN
afs-72443	106	25	.	.	PUNCT
afs-72443	107	1	in	in	ADP
afs-72443	107	2	the	the	DET
afs-72443	107	3	mouse	mouse	NOUN
afs-72443	107	4	,	,	PUNCT
afs-72443	107	5	two	two	NUM
afs-72443	107	6	fetuses	fetus	NOUN
afs-72443	107	7	which	which	PRON
afs-72443	107	8	were	be	AUX
afs-72443	107	9	sexed	sex	VERB
afs-72443	107	10	at	at	ADP
afs-72443	107	11	the	the	DET
afs-72443	107	12	preimplantation	preimplantation	NOUN
afs-72443	107	13	stage	stage	NOUN
afs-72443	107	14	were	be	AUX
afs-72443	107	15	recovered	recover	VERB
afs-72443	107	16	by	by	ADP
afs-72443	107	17	caesarean	caesarean	ADJ
afs-72443	107	18	section	section	NOUN
afs-72443	107	19	,	,	PUNCT
afs-72443	107	20	both	both	PRON
afs-72443	107	21	being	be	AUX
afs-72443	107	22	correctly	correctly	ADV
afs-72443	107	23	diagnosed	diagnose	VERB
afs-72443	107	24	(	(	PUNCT
afs-72443	107	25	kananen	kananen	NOUN
afs-72443	107	26	,	,	PUNCT
afs-72443	107	27	in	in	ADP
afs-72443	107	28	preparation	preparation	NOUN
afs-72443	107	29	)	)	PUNCT
afs-72443	107	30	.	.	PUNCT
afs-72443	108	1	thus	thus	ADV
afs-72443	108	2	,	,	PUNCT
afs-72443	108	3	the	the	DET
afs-72443	108	4	method	method	NOUN
afs-72443	108	5	appears	appear	VERB
afs-72443	108	6	to	to	PART
afs-72443	108	7	be	be	AUX
afs-72443	108	8	highly	highly	ADV
afs-72443	108	9	accurate	accurate	ADJ
afs-72443	108	10	,	,	PUNCT
afs-72443	108	11	and	and	CCONJ
afs-72443	108	12	with	with	ADP
afs-72443	108	13	proper	proper	ADJ
afs-72443	108	14	handling	handling	NOUN
afs-72443	108	15	of	of	ADP
afs-72443	108	16	biopsies	biopsy	NOUN
afs-72443	108	17	most	most	ADJ
afs-72443	108	18	samples	sample	NOUN
afs-72443	108	19	can	can	AUX
afs-72443	108	20	be	be	AUX
afs-72443	108	21	diagnosed	diagnose	VERB
afs-72443	108	22	.	.	PUNCT
afs-72443	109	1	fig	fig	NOUN
afs-72443	109	2	.	.	PUNCT
afs-72443	110	1	1	1	X
afs-72443	110	2	.	.	X
afs-72443	110	3	banding	band	VERB
afs-72443	110	4	patterns	pattern	NOUN
afs-72443	110	5	from	from	ADP
afs-72443	110	6	digested	digest	VERB
afs-72443	110	7	zfy	zfy	PROPN
afs-72443	110	8	/	/	SYM
afs-72443	110	9	zfx	zfx	NOUN
afs-72443	110	10	pcr	pcr	NOUN
afs-72443	110	11	products	product	NOUN
afs-72443	110	12	of	of	ADP
afs-72443	110	13	male	male	NOUN
afs-72443	110	14	(	(	PUNCT
afs-72443	110	15	lanes	lane	NOUN
afs-72443	110	16	2,3	2,3	NUM
afs-72443	110	17	,	,	PUNCT
afs-72443	110	18	7	7	NUM
afs-72443	110	19	)	)	PUNCT
afs-72443	110	20	and	and	CCONJ
afs-72443	110	21	female	female	ADJ
afs-72443	110	22	sheep	sheep	NOUN
afs-72443	110	23	embryo	embryo	NOUN
afs-72443	110	24	biopsies	biopsy	NOUN
afs-72443	110	25	(	(	PUNCT
afs-72443	110	26	lanes	lane	NOUN
afs-72443	110	27	4	4	NUM
afs-72443	110	28	,	,	PUNCT
afs-72443	110	29	5,6	5,6	NUM
afs-72443	110	30	)	)	PUNCT
afs-72443	110	31	.	.	PUNCT
afs-72443	111	1	samples	sample	NOUN
afs-72443	111	2	in	in	ADP
afs-72443	111	3	lanes	lane	NOUN
afs-72443	111	4	2	2	NUM
afs-72443	111	5	-	-	SYM
afs-72443	111	6	7	7	NUM
afs-72443	111	7	were	be	AUX
afs-72443	111	8	from	from	ADP
afs-72443	111	9	5,3	5,3	NUM
afs-72443	111	10	,	,	PUNCT
afs-72443	111	11	7	7	NUM
afs-72443	111	12	and	and	CCONJ
afs-72443	111	13	3	3	NUM
afs-72443	111	14	blastomeres	blastomere	NOUN
afs-72443	111	15	,	,	PUNCT
afs-72443	111	16	a	a	DET
afs-72443	111	17	quarter	quarter	NOUN
afs-72443	111	18	embryo	embryo	NOUN
afs-72443	111	19	,	,	PUNCT
afs-72443	111	20	and	and	CCONJ
afs-72443	111	21	a	a	DET
afs-72443	111	22	half	half	ADJ
afs-72443	111	23	embryo	embryo	NOUN
afs-72443	111	24	,	,	PUNCT
afs-72443	111	25	respectively	respectively	ADV
afs-72443	111	26	.	.	PUNCT
afs-72443	112	1	samples	sample	NOUN
afs-72443	112	2	6	6	NUM
afs-72443	112	3	and	and	CCONJ
afs-72443	112	4	7	7	NUM
afs-72443	112	5	were	be	AUX
afs-72443	112	6	prepared	prepare	VERB
afs-72443	112	7	under	under	ADP
afs-72443	112	8	farm	farm	NOUN
afs-72443	112	9	conditions	condition	NOUN
afs-72443	112	10	.	.	PUNCT
afs-72443	113	1	lanes	lane	NOUN
afs-72443	113	2	1	1	NUM
afs-72443	113	3	and	and	CCONJ
afs-72443	113	4	8	8	NUM
afs-72443	113	5	,	,	PUNCT
afs-72443	113	6	molecular	molecular	ADJ
afs-72443	113	7	weight	weight	NOUN
afs-72443	113	8	markers	marker	NOUN
afs-72443	113	9	.	.	PUNCT
afs-72443	114	1	fig	fig	NOUN
afs-72443	114	2	.	.	PUNCT
afs-72443	115	1	2	2	X
afs-72443	115	2	.	.	X
afs-72443	115	3	banding	band	VERB
afs-72443	115	4	patterns	pattern	NOUN
afs-72443	115	5	from	from	ADP
afs-72443	115	6	bovine	bovine	ADJ
afs-72443	115	7	male	male	NOUN
afs-72443	115	8	(	(	PUNCT
afs-72443	115	9	lanes	lane	NOUN
afs-72443	115	10	1	1	NUM
afs-72443	115	11	and	and	CCONJ
afs-72443	115	12	3	3	NUM
afs-72443	115	13	)	)	PUNCT
afs-72443	115	14	and	and	CCONJ
afs-72443	115	15	female	female	ADJ
afs-72443	115	16	(	(	PUNCT
afs-72443	115	17	lanes	lane	NOUN
afs-72443	115	18	2	2	NUM
afs-72443	115	19	and	and	CCONJ
afs-72443	115	20	4	4	NUM
afs-72443	115	21	)	)	PUNCT
afs-72443	115	22	zfy	zfy	PROPN
afs-72443	115	23	/	/	SYM
afs-72443	115	24	zfx	zfx	NOUN
afs-72443	115	25	pcr	pcr	NOUN
afs-72443	115	26	products	product	NOUN
afs-72443	115	27	.	.	PUNCT
afs-72443	116	1	male	male	ADJ
afs-72443	116	2	samples	sample	NOUN
afs-72443	116	3	included	include	VERB
afs-72443	116	4	10	10	NUM
afs-72443	116	5	ng	ng	PROPN
afs-72443	116	6	(	(	PUNCT
afs-72443	116	7	lane	lane	NOUN
afs-72443	116	8	1	1	NUM
afs-72443	116	9	)	)	PUNCT
afs-72443	116	10	and	and	CCONJ
afs-72443	116	11	1	1	NUM
afs-72443	116	12	ng	ng	PROPN
afs-72443	116	13	(	(	PUNCT
afs-72443	116	14	lane	lane	NOUN
afs-72443	116	15	3	3	NUM
afs-72443	116	16	)	)	PUNCT
afs-72443	116	17	of	of	ADP
afs-72443	116	18	dna	dna	PROPN
afs-72443	116	19	.	.	PUNCT
afs-72443	117	1	both	both	DET
afs-72443	117	2	female	female	ADJ
afs-72443	117	3	patterns	pattern	NOUN
afs-72443	117	4	are	be	AUX
afs-72443	117	5	from	from	ADP
afs-72443	117	6	two	two	NUM
afs-72443	117	7	-	-	PUNCT
afs-72443	117	8	cell	cell	NOUN
afs-72443	117	9	-	-	PUNCT
afs-72443	117	10	stage	stage	NOUN
afs-72443	117	11	embryos	embryos	NOUN
afs-72443	117	12	.	.	PUNCT
afs-72443	118	1	lane	lane	PROPN
afs-72443	118	2	5	5	NUM
afs-72443	118	3	,	,	PUNCT
afs-72443	118	4	molecular	molecular	ADJ
afs-72443	118	5	weight	weight	NOUN
afs-72443	118	6	marker	marker	NOUN
afs-72443	118	7	.	.	PUNCT
afs-72443	119	1	236	236	NUM
afs-72443	119	2	agric	agric	NOUN
afs-72443	119	3	.	.	PUNCT
afs-72443	120	1	sei	sei	ADJ
afs-72443	120	2	.	.	PUNCT
afs-72443	120	3	finl	finl	NOUN
afs-72443	120	4	.	.	PUNCT
afs-72443	121	1	1	1	NUM
afs-72443	121	2	(	(	PUNCT
afs-72443	121	3	1992	1992	NUM
afs-72443	121	4	)	)	PUNCT
afs-72443	121	5	since	since	SCONJ
afs-72443	121	6	the	the	DET
afs-72443	121	7	zfy	zfy	PROPN
afs-72443	121	8	locus	locus	NOUN
afs-72443	121	9	is	be	AUX
afs-72443	121	10	not	not	PART
afs-72443	121	11	the	the	DET
afs-72443	121	12	testis	testis	NOUN
afs-72443	121	13	determining	determine	VERB
afs-72443	121	14	gene	gene	NOUN
afs-72443	121	15	,	,	PUNCT
afs-72443	121	16	there	there	PRON
afs-72443	121	17	is	be	VERB
afs-72443	121	18	a	a	DET
afs-72443	121	19	minor	minor	ADJ
afs-72443	121	20	risk	risk	NOUN
afs-72443	121	21	of	of	ADP
afs-72443	121	22	chromosomal	chromosomal	ADJ
afs-72443	121	23	rearrangements	rearrangement	NOUN
afs-72443	121	24	leading	lead	VERB
afs-72443	121	25	to	to	ADP
afs-72443	121	26	zfy	zfy	ADJ
afs-72443	121	27	-	-	PUNCT
afs-72443	121	28	positive	positive	ADJ
afs-72443	121	29	females	female	NOUN
afs-72443	121	30	or	or	CCONJ
afs-72443	121	31	vice	vice	NOUN
afs-72443	121	32	versa	versa	ADV
afs-72443	121	33	.	.	PUNCT
afs-72443	122	1	caution	caution	NOUN
afs-72443	122	2	must	must	AUX
afs-72443	122	3	be	be	AUX
afs-72443	122	4	exercised	exercise	VERB
afs-72443	122	5	to	to	PART
afs-72443	122	6	avoid	avoid	VERB
afs-72443	122	7	contamination	contamination	NOUN
afs-72443	122	8	of	of	ADP
afs-72443	122	9	any	any	DET
afs-72443	122	10	source	source	NOUN
afs-72443	122	11	ofplacental	ofplacental	ADJ
afs-72443	122	12	mammaliandna	mammaliandna	PROPN
afs-72443	122	13	,	,	PUNCT
afs-72443	122	14	since	since	SCONJ
afs-72443	122	15	the	the	DET
afs-72443	122	16	distinctionbetween	distinctionbetween	ADJ
afs-72443	122	17	sexes	sex	NOUN
afs-72443	122	18	is	be	AUX
afs-72443	122	19	dependent	dependent	ADJ
afs-72443	122	20	on	on	ADP
afs-72443	122	21	species	specie	NOUN
afs-72443	122	22	-	-	PUNCT
afs-72443	122	23	specific	specific	ADJ
afs-72443	122	24	restriction	restriction	NOUN
afs-72443	122	25	enzymes	enzyme	NOUN
afs-72443	122	26	.	.	PUNCT
afs-72443	123	1	for	for	ADP
afs-72443	123	2	instance	instance	NOUN
afs-72443	123	3	,	,	PUNCT
afs-72443	123	4	if	if	SCONJ
afs-72443	123	5	murine	murine	ADJ
afs-72443	123	6	samples	sample	NOUN
afs-72443	123	7	are	be	AUX
afs-72443	123	8	contaminated	contaminate	VERB
afs-72443	123	9	with	with	ADP
afs-72443	123	10	bovine	bovine	PROPN
afs-72443	123	11	dna	dna	PROPN
afs-72443	123	12	,	,	PUNCT
afs-72443	123	13	females	female	NOUN
afs-72443	123	14	will	will	AUX
afs-72443	123	15	be	be	AUX
afs-72443	123	16	identified	identify	VERB
afs-72443	123	17	as	as	ADP
afs-72443	123	18	males	male	NOUN
afs-72443	123	19	,	,	PUNCT
afs-72443	123	20	since	since	SCONJ
afs-72443	123	21	bovine	bovine	PROPN
afs-72443	123	22	dna	dna	PROPN
afs-72443	123	23	remains	remain	VERB
afs-72443	123	24	undigested	undigested	ADJ
afs-72443	123	25	withhaelll	withhaelll	NOUN
afs-72443	123	26	.	.	PUNCT
afs-72443	124	1	also	also	ADV
afs-72443	124	2	,	,	PUNCT
afs-72443	124	3	fetal	fetal	ADJ
afs-72443	124	4	calf	calf	NOUN
afs-72443	124	5	serum	serum	NOUN
afs-72443	124	6	(	(	PUNCT
afs-72443	124	7	or	or	CCONJ
afs-72443	124	8	bovine	bovine	VERB
afs-72443	124	9	serum	serum	NOUN
afs-72443	124	10	albumin	albumin	NOUN
afs-72443	124	11	)	)	PUNCT
afs-72443	124	12	may	may	AUX
afs-72443	124	13	contain	contain	VERB
afs-72443	124	14	trace	trace	NOUN
afs-72443	124	15	amounts	amount	NOUN
afs-72443	124	16	of	of	ADP
afs-72443	124	17	dna	dna	NOUN
afs-72443	124	18	resulting	result	VERB
afs-72443	124	19	in	in	ADP
afs-72443	124	20	amplification	amplification	NOUN
afs-72443	124	21	products	product	NOUN
afs-72443	124	22	.	.	PUNCT
afs-72443	125	1	preliminary	preliminary	ADJ
afs-72443	125	2	trials	trial	NOUN
afs-72443	125	3	suggest	suggest	VERB
afs-72443	125	4	that	that	SCONJ
afs-72443	125	5	this	this	DET
afs-72443	125	6	problem	problem	NOUN
afs-72443	125	7	can	can	AUX
afs-72443	125	8	be	be	AUX
afs-72443	125	9	overcome	overcome	VERB
afs-72443	125	10	by	by	ADP
afs-72443	125	11	exposure	exposure	NOUN
afs-72443	125	12	to	to	ADP
afs-72443	125	13	uv	uv	NOUN
afs-72443	125	14	light	light	NOUN
afs-72443	125	15	(	(	PUNCT
afs-72443	125	16	peippo	peippo	PROPN
afs-72443	125	17	and	and	CCONJ
afs-72443	125	18	bredbacka	bredbacka	PROPN
afs-72443	125	19	,	,	PUNCT
afs-72443	125	20	unpublished	unpublished	ADJ
afs-72443	125	21	)	)	PUNCT
afs-72443	125	22	.	.	PUNCT
afs-72443	126	1	however	however	ADV
afs-72443	126	2	,	,	PUNCT
afs-72443	126	3	the	the	DET
afs-72443	126	4	results	result	NOUN
afs-72443	126	5	presented	present	VERB
afs-72443	126	6	in	in	ADP
afs-72443	126	7	this	this	DET
afs-72443	126	8	paper	paper	NOUN
afs-72443	126	9	would	would	AUX
afs-72443	126	10	suggest	suggest	VERB
afs-72443	126	11	that	that	SCONJ
afs-72443	126	12	sample	sample	NOUN
afs-72443	126	13	contamination	contamination	NOUN
afs-72443	126	14	is	be	AUX
afs-72443	126	15	not	not	PART
afs-72443	126	16	a	a	DET
afs-72443	126	17	major	major	ADJ
afs-72443	126	18	problem	problem	NOUN
afs-72443	126	19	.	.	PUNCT
afs-72443	127	1	compared	compare	VERB
afs-72443	127	2	to	to	ADP
afs-72443	127	3	the	the	DET
afs-72443	127	4	approaches	approach	NOUN
afs-72443	127	5	involving	involve	VERB
afs-72443	127	6	control	control	NOUN
afs-72443	127	7	primers	primer	NOUN
afs-72443	127	8	,	,	PUNCT
afs-72443	127	9	the	the	DET
afs-72443	127	10	time	time	NOUN
afs-72443	127	11	required	require	VERB
afs-72443	127	12	for	for	ADP
afs-72443	127	13	restriction	restriction	NOUN
afs-72443	127	14	enzyme	enzyme	NOUN
afs-72443	127	15	digestion	digestion	NOUN
afs-72443	127	16	is	be	AUX
afs-72443	127	17	a	a	DET
afs-72443	127	18	disadvantage	disadvantage	NOUN
afs-72443	127	19	of	of	ADP
afs-72443	127	20	the	the	DET
afs-72443	127	21	protocol	protocol	NOUN
afs-72443	127	22	presented	present	VERB
afs-72443	127	23	in	in	ADP
afs-72443	127	24	this	this	DET
afs-72443	127	25	paper	paper	NOUN
afs-72443	127	26	.	.	PUNCT
afs-72443	128	1	however	however	ADV
afs-72443	128	2	,	,	PUNCT
afs-72443	128	3	in	in	ADP
afs-72443	128	4	this	this	DET
afs-72443	128	5	study	study	NOUN
afs-72443	128	6	we	we	PRON
afs-72443	128	7	also	also	ADV
afs-72443	128	8	examined	examine	VERB
afs-72443	128	9	a	a	DET
afs-72443	128	10	faster	fast	ADJ
afs-72443	128	11	technique	technique	NOUN
afs-72443	128	12	to	to	ADP
afs-72443	128	13	lyse	lyse	NOUN
afs-72443	128	14	embryonic	embryonic	ADJ
afs-72443	128	15	cells	cell	NOUN
afs-72443	128	16	,	,	PUNCT
afs-72443	128	17	utilizing	utilize	VERB
afs-72443	128	18	alkaline	alkaline	NOUN
afs-72443	128	19	treatment	treatment	NOUN
afs-72443	128	20	at	at	ADP
afs-72443	128	21	65	65	NUM
afs-72443	128	22	°	°	NOUN
afs-72443	128	23	c	c	NOUN
afs-72443	128	24	.	.	PUNCT
afs-72443	129	1	this	this	DET
afs-72443	129	2	chemical	chemical	NOUN
afs-72443	129	3	approach	approach	NOUN
afs-72443	129	4	saves	save	VERB
afs-72443	129	5	about	about	ADV
afs-72443	129	6	one	one	NUM
afs-72443	129	7	hour	hour	NOUN
afs-72443	129	8	compared	compare	VERB
afs-72443	129	9	to	to	ADP
afs-72443	129	10	the	the	DET
afs-72443	129	11	enzymatic	enzymatic	ADJ
afs-72443	129	12	method	method	NOUN
afs-72443	129	13	,	,	PUNCT
afs-72443	129	14	but	but	CCONJ
afs-72443	129	15	needs	need	VERB
afs-72443	129	16	further	further	ADJ
afs-72443	129	17	optimization	optimization	NOUN
afs-72443	129	18	since	since	SCONJ
afs-72443	129	19	only	only	ADV
afs-72443	129	20	about	about	ADV
afs-72443	129	21	one	one	NUM
afs-72443	129	22	third	third	NOUN
afs-72443	129	23	of	of	ADP
afs-72443	129	24	the	the	DET
afs-72443	129	25	samples	sample	NOUN
afs-72443	129	26	yielded	yield	VERB
afs-72443	129	27	amplification	amplification	NOUN
afs-72443	129	28	products	product	NOUN
afs-72443	129	29	in	in	ADP
afs-72443	129	30	this	this	DET
afs-72443	129	31	study	study	NOUN
afs-72443	129	32	.	.	PUNCT
afs-72443	130	1	with	with	ADP
afs-72443	130	2	the	the	DET
afs-72443	130	3	presented	present	VERB
afs-72443	130	4	protocol	protocol	NOUN
afs-72443	130	5	,	,	PUNCT
afs-72443	130	6	embryo	embryo	NOUN
afs-72443	130	7	sexing	sexing	NOUN
afs-72443	130	8	can	can	AUX
afs-72443	130	9	be	be	AUX
afs-72443	130	10	performed	perform	VERB
afs-72443	130	11	in	in	ADP
afs-72443	130	12	about	about	ADV
afs-72443	130	13	five	five	NUM
afs-72443	130	14	hours	hour	NOUN
afs-72443	130	15	when	when	SCONJ
afs-72443	130	16	alkaline	alkaline	ADJ
afs-72443	130	17	lysis	lysis	NOUN
afs-72443	130	18	of	of	ADP
afs-72443	130	19	samples	sample	NOUN
afs-72443	130	20	is	be	AUX
afs-72443	130	21	utilized	utilize	VERB
afs-72443	130	22	.	.	PUNCT
afs-72443	131	1	with	with	ADP
afs-72443	131	2	faster	fast	ADJ
afs-72443	131	3	temperature	temperature	NOUN
afs-72443	131	4	cycling	cycling	NOUN
afs-72443	131	5	it	it	PRON
afs-72443	131	6	is	be	AUX
afs-72443	131	7	very	very	ADV
afs-72443	131	8	likely	likely	ADJ
afs-72443	131	9	that	that	SCONJ
afs-72443	131	10	the	the	DET
afs-72443	131	11	protocol	protocol	NOUN
afs-72443	131	12	can	can	AUX
afs-72443	131	13	be	be	AUX
afs-72443	131	14	shortened	shorten	VERB
afs-72443	131	15	even	even	ADV
afs-72443	131	16	more	more	ADV
afs-72443	131	17	,	,	PUNCT
afs-72443	131	18	thus	thus	ADV
afs-72443	131	19	making	make	VERB
afs-72443	131	20	embryo	embryo	NOUN
afs-72443	131	21	sexing	sex	VERB
afs-72443	131	22	under	under	ADP
afs-72443	131	23	field	field	NOUN
afs-72443	131	24	conditions	condition	NOUN
afs-72443	131	25	more	more	ADV
afs-72443	131	26	practical	practical	ADJ
afs-72443	131	27	.	.	PUNCT
afs-72443	132	1	since	since	SCONJ
afs-72443	132	2	the	the	DET
afs-72443	132	3	primers	primer	NOUN
afs-72443	132	4	have	have	VERB
afs-72443	132	5	a	a	DET
afs-72443	132	6	universal	universal	ADJ
afs-72443	132	7	nature	nature	NOUN
afs-72443	132	8	,	,	PUNCT
afs-72443	132	9	initial	initial	ADJ
afs-72443	132	10	testing	testing	NOUN
afs-72443	132	11	can	can	AUX
afs-72443	132	12	be	be	AUX
afs-72443	132	13	performed	perform	VERB
afs-72443	132	14	with	with	ADP
afs-72443	132	15	mouse	mouse	NOUN
afs-72443	132	16	embryos	embryos	NOUN
afs-72443	132	17	.	.	PUNCT
afs-72443	133	1	it	it	PRON
afs-72443	133	2	is	be	AUX
afs-72443	133	3	quite	quite	ADV
afs-72443	133	4	possible	possible	ADJ
afs-72443	133	5	that	that	SCONJ
afs-72443	133	6	different	different	ADJ
afs-72443	133	7	laboratories	laboratory	NOUN
afs-72443	133	8	may	may	AUX
afs-72443	133	9	need	need	VERB
afs-72443	133	10	to	to	PART
afs-72443	133	11	adopt	adopt	VERB
afs-72443	133	12	some	some	DET
afs-72443	133	13	minor	minor	ADJ
afs-72443	133	14	modifications	modification	NOUN
afs-72443	133	15	of	of	ADP
afs-72443	133	16	this	this	DET
afs-72443	133	17	protocol	protocol	NOUN
afs-72443	133	18	.	.	PUNCT
afs-72443	134	1	therefore	therefore	ADV
afs-72443	134	2	the	the	DET
afs-72443	134	3	possibility	possibility	NOUN
afs-72443	134	4	of	of	ADP
afs-72443	134	5	using	use	VERB
afs-72443	134	6	mouse	mouse	NOUN
afs-72443	134	7	embryos	embryo	NOUN
afs-72443	134	8	in	in	ADP
afs-72443	134	9	the	the	DET
afs-72443	134	10	optimization	optimization	NOUN
afs-72443	134	11	process	process	NOUN
afs-72443	134	12	is	be	AUX
afs-72443	134	13	an	an	DET
afs-72443	134	14	advantage	advantage	NOUN
afs-72443	134	15	,	,	PUNCT
afs-72443	134	16	particularity	particularity	NOUN
afs-72443	134	17	when	when	SCONJ
afs-72443	134	18	the	the	DET
afs-72443	134	19	sheep	sheep	NOUN
afs-72443	134	20	embryo	embryo	NOUN
afs-72443	134	21	sexing	sex	VERB
afs-72443	134	22	protocol	protocol	NOUN
afs-72443	134	23	is	be	AUX
afs-72443	134	24	being	be	AUX
afs-72443	134	25	optimized	optimize	VERB
afs-72443	134	26	,	,	PUNCT
afs-72443	134	27	since	since	SCONJ
afs-72443	134	28	both	both	DET
afs-72443	134	29	species	specie	NOUN
afs-72443	134	30	can	can	AUX
afs-72443	134	31	be	be	AUX
afs-72443	134	32	sexed	sex	VERB
afs-72443	134	33	with	with	ADP
afs-72443	134	34	the	the	DET
afs-72443	134	35	same	same	ADJ
afs-72443	134	36	protocol	protocol	NOUN
afs-72443	134	37	.	.	PUNCT
afs-72443	135	1	it	it	PRON
afs-72443	135	2	may	may	AUX
afs-72443	135	3	be	be	AUX
afs-72443	135	4	possible	possible	ADJ
afs-72443	135	5	to	to	PART
afs-72443	135	6	test	test	VERB
afs-72443	135	7	the	the	DET
afs-72443	135	8	conditions	condition	NOUN
afs-72443	135	9	with	with	ADP
afs-72443	135	10	blood	blood	NOUN
afs-72443	135	11	or	or	CCONJ
afs-72443	135	12	any	any	DET
afs-72443	135	13	other	other	ADJ
afs-72443	135	14	source	source	NOUN
afs-72443	135	15	of	of	ADP
afs-72443	135	16	dna	dna	PROPN
afs-72443	135	17	,	,	PUNCT
afs-72443	135	18	but	but	CCONJ
afs-72443	135	19	there	there	PRON
afs-72443	135	20	are	be	VERB
afs-72443	135	21	some	some	DET
afs-72443	135	22	limitations	limitation	NOUN
afs-72443	135	23	to	to	ADP
afs-72443	135	24	this	this	PRON
afs-72443	135	25	.	.	PUNCT
afs-72443	136	1	for	for	ADP
afs-72443	136	2	instance	instance	NOUN
afs-72443	136	3	,	,	PUNCT
afs-72443	136	4	the	the	DET
afs-72443	136	5	control	control	NOUN
afs-72443	136	6	of	of	ADP
afs-72443	136	7	the	the	DET
afs-72443	136	8	amount	amount	NOUN
afs-72443	136	9	of	of	ADP
afs-72443	136	10	template	template	NOUN
afs-72443	136	11	is	be	AUX
afs-72443	136	12	better	well	ADJ
afs-72443	136	13	with	with	ADP
afs-72443	136	14	embryos	embryo	NOUN
afs-72443	136	15	or	or	CCONJ
afs-72443	136	16	embryonic	embryonic	ADJ
afs-72443	136	17	cells	cell	NOUN
afs-72443	136	18	than	than	ADP
afs-72443	136	19	with	with	ADP
afs-72443	136	20	a	a	DET
afs-72443	136	21	highly	highly	ADV
afs-72443	136	22	diluted	dilute	VERB
afs-72443	136	23	dna	dna	NOUN
afs-72443	136	24	solution	solution	NOUN
afs-72443	136	25	,	,	PUNCT
afs-72443	136	26	which	which	PRON
afs-72443	136	27	at	at	ADP
afs-72443	136	28	very	very	ADV
afs-72443	136	29	low	low	ADJ
afs-72443	136	30	concentrations	concentration	NOUN
afs-72443	136	31	may	may	AUX
afs-72443	136	32	totally	totally	ADV
afs-72443	136	33	lack	lack	VERB
afs-72443	136	34	the	the	DET
afs-72443	136	35	template	template	NOUN
afs-72443	136	36	sequence	sequence	NOUN
afs-72443	136	37	.	.	PUNCT
afs-72443	137	1	furthermore	furthermore	ADV
afs-72443	137	2	,	,	PUNCT
afs-72443	137	3	embryonic	embryonic	ADJ
afs-72443	137	4	cells	cell	NOUN
afs-72443	137	5	appear	appear	VERB
afs-72443	137	6	to	to	PART
afs-72443	137	7	be	be	AUX
afs-72443	137	8	more	more	ADV
afs-72443	137	9	resistant	resistant	ADJ
afs-72443	137	10	to	to	PART
afs-72443	137	11	lysis	lysis	VERB
afs-72443	137	12	than	than	ADP
afs-72443	137	13	many	many	ADJ
afs-72443	137	14	other	other	ADJ
afs-72443	137	15	cell	cell	NOUN
afs-72443	137	16	types	type	NOUN
afs-72443	137	17	.	.	PUNCT
afs-72443	138	1	basically	basically	ADV
afs-72443	138	2	embryos	embryo	NOUN
afs-72443	138	3	from	from	ADP
afs-72443	138	4	any	any	DET
afs-72443	138	5	mammalian	mammalian	ADJ
afs-72443	138	6	species	specie	NOUN
afs-72443	138	7	could	could	AUX
afs-72443	138	8	be	be	AUX
afs-72443	138	9	sexed	sex	VERB
afs-72443	138	10	by	by	ADP
afs-72443	138	11	the	the	DET
afs-72443	138	12	method	method	NOUN
afs-72443	138	13	presented	present	VERB
afs-72443	138	14	in	in	ADP
afs-72443	138	15	this	this	DET
afs-72443	138	16	study	study	NOUN
afs-72443	138	17	,	,	PUNCT
afs-72443	138	18	provided	provide	VERB
afs-72443	138	19	that	that	SCONJ
afs-72443	138	20	sequence	sequence	NOUN
afs-72443	138	21	differences	difference	NOUN
afs-72443	138	22	exist	exist	VERB
afs-72443	138	23	between	between	ADP
afs-72443	138	24	the	the	DET
afs-72443	138	25	xand	xand	PROPN
afs-72443	138	26	y	y	PROPN
afs-72443	138	27	-	-	ADJ
afs-72443	138	28	chromosomal	chromosomal	ADJ
afs-72443	138	29	products	product	NOUN
afs-72443	138	30	.	.	PUNCT
afs-72443	139	1	in	in	ADP
afs-72443	139	2	ideal	ideal	ADJ
afs-72443	139	3	situations	situation	NOUN
afs-72443	139	4	these	these	DET
afs-72443	139	5	differences	difference	NOUN
afs-72443	139	6	are	be	AUX
afs-72443	139	7	recognized	recognize	VERB
afs-72443	139	8	by	by	ADP
afs-72443	139	9	restriction	restriction	NOUN
afs-72443	139	10	enzymes	enzyme	NOUN
afs-72443	139	11	.	.	PUNCT
afs-72443	140	1	using	use	VERB
afs-72443	140	2	a	a	DET
afs-72443	140	3	variety	variety	NOUN
afs-72443	140	4	of	of	ADP
afs-72443	140	5	enzymes	enzyme	NOUN
afs-72443	140	6	,	,	PUNCT
afs-72443	140	7	aasen	aasen	NOUN
afs-72443	140	8	and	and	CCONJ
afs-72443	140	9	medrano	medrano	NOUN
afs-72443	140	10	(	(	PUNCT
afs-72443	140	11	1990	1990	NUM
afs-72443	140	12	)	)	PUNCT
afs-72443	140	13	showed	show	VERB
afs-72443	140	14	rflp	rflp	PROPN
afs-72443	140	15	differences	difference	NOUN
afs-72443	140	16	between	between	ADP
afs-72443	140	17	the	the	DET
afs-72443	140	18	sexes	sex	NOUN
afs-72443	140	19	in	in	ADP
afs-72443	140	20	cattle	cattle	NOUN
afs-72443	140	21	,	,	PUNCT
afs-72443	140	22	sheep	sheep	NOUN
afs-72443	140	23	,	,	PUNCT
afs-72443	140	24	goat	goat	PROPN
afs-72443	140	25	and	and	CCONJ
afs-72443	140	26	humans	human	NOUN
afs-72443	140	27	,	,	PUNCT
afs-72443	140	28	but	but	CCONJ
afs-72443	140	29	the	the	DET
afs-72443	140	30	rflps	rflp	NOUN
afs-72443	140	31	were	be	AUX
afs-72443	140	32	identical	identical	ADJ
afs-72443	140	33	between	between	ADP
afs-72443	140	34	the	the	DET
afs-72443	140	35	sexes	sex	NOUN
afs-72443	140	36	in	in	ADP
afs-72443	140	37	the	the	DET
afs-72443	140	38	horse	horse	NOUN
afs-72443	140	39	,	,	PUNCT
afs-72443	140	40	pig	pig	NOUN
afs-72443	140	41	and	and	CCONJ
afs-72443	140	42	rainbow	rainbow	NOUN
afs-72443	140	43	trout	trout	NOUN
afs-72443	140	44	.	.	PUNCT
afs-72443	141	1	however	however	ADV
afs-72443	141	2	,	,	PUNCT
afs-72443	141	3	polymorphisms	polymorphism	NOUN
afs-72443	141	4	thatremain	thatremain	NOUN
afs-72443	141	5	unobserved	unobserved	ADJ
afs-72443	141	6	after	after	SCONJ
afs-72443	141	7	rflp	rflp	NOUN
afs-72443	141	8	analysis	analysis	NOUN
afs-72443	141	9	could	could	AUX
afs-72443	141	10	be	be	AUX
afs-72443	141	11	detected	detect	VERB
afs-72443	141	12	on	on	ADP
afs-72443	141	13	the	the	DET
afs-72443	141	14	basis	basis	NOUN
afs-72443	141	15	of	of	ADP
afs-72443	141	16	differential	differential	ADJ
afs-72443	141	17	hybridization	hybridization	NOUN
afs-72443	141	18	of	of	ADP
afs-72443	141	19	allele	allele	PROPN
afs-72443	141	20	/	/	SYM
afs-72443	141	21	homologue	homologue	NOUN
afs-72443	141	22	specific	specific	ADJ
afs-72443	141	23	oligonucleotides	oligonucleotide	NOUN
afs-72443	141	24	.	.	PUNCT
afs-72443	142	1	this	this	PRON
afs-72443	142	2	would	would	AUX
afs-72443	142	3	require	require	VERB
afs-72443	142	4	sequence	sequence	NOUN
afs-72443	142	5	information	information	NOUN
afs-72443	142	6	of	of	ADP
afs-72443	142	7	the	the	DET
afs-72443	142	8	zfy	zfy	NOUN
afs-72443	142	9	and	and	CCONJ
afs-72443	142	10	zfx	zfx	NOUN
afs-72443	142	11	loci	loci	NOUN
afs-72443	142	12	.	.	PUNCT
afs-72443	143	1	acknowledgements	acknowledgement	NOUN
afs-72443	143	2	.	.	PUNCT
afs-72443	144	1	the	the	DET
afs-72443	144	2	authors	author	NOUN
afs-72443	144	3	wish	wish	VERB
afs-72443	144	4	to	to	PART
afs-72443	144	5	thank	thank	VERB
afs-72443	144	6	johanna	johanna	PROPN
afs-72443	144	7	viikki	viikki	PROPN
afs-72443	144	8	for	for	ADP
afs-72443	144	9	synthesizing	synthesize	VERB
afs-72443	144	10	the	the	DET
afs-72443	144	11	oligonucleotides	oligonucleotide	NOUN
afs-72443	144	12	and	and	CCONJ
afs-72443	144	13	riikka	riikka	NOUN
afs-72443	144	14	velmala	velmala	NOUN
afs-72443	144	15	for	for	ADP
afs-72443	144	16	extracting	extract	VERB
afs-72443	144	17	dna	dna	NOUN
afs-72443	144	18	from	from	ADP
afs-72443	144	19	the	the	DET
afs-72443	144	20	blood	blood	NOUN
afs-72443	144	21	samples	sample	NOUN
afs-72443	144	22	.	.	PUNCT
afs-72443	145	1	the	the	DET
afs-72443	145	2	finnish	finnish	ADJ
afs-72443	145	3	-	-	PUNCT
afs-72443	145	4	egyptian	egyptian	ADJ
afs-72443	145	5	sheep	sheep	NOUN
afs-72443	145	6	breeding	breeding	NOUN
afs-72443	145	7	project	project	NOUN
afs-72443	145	8	provided	provide	VERB
afs-72443	145	9	all	all	DET
afs-72443	145	10	the	the	DET
afs-72443	145	11	sheep	sheep	NOUN
afs-72443	145	12	embryos	embryo	NOUN
afs-72443	145	13	used	use	VERB
afs-72443	145	14	in	in	ADP
afs-72443	145	15	this	this	DET
afs-72443	145	16	study	study	NOUN
afs-72443	145	17	.	.	PUNCT
afs-72443	146	1	references	reference	NOUN
afs-72443	146	2	aasen	aasen	PROPN
afs-72443	146	3	,	,	PUNCT
afs-72443	146	4	e.	e.	PROPN
afs-72443	146	5	&	&	CCONJ
afs-72443	146	6	medrano	medrano	PROPN
afs-72443	146	7	,	,	PUNCT
afs-72443	146	8	j.f	j.f	PROPN
afs-72443	146	9	.	.	PROPN
afs-72443	146	10	1990	1990	NUM
afs-72443	146	11	.	.	PUNCT
afs-72443	147	1	amplification	amplification	NOUN
afs-72443	147	2	ofthe	ofthe	PRON
afs-72443	147	3	zfy	zfy	NOUN
afs-72443	147	4	and	and	CCONJ
afs-72443	147	5	zfx	zfx	NOUN
afs-72443	147	6	genes	gene	NOUN
afs-72443	147	7	for	for	ADP
afs-72443	147	8	sex	sex	NOUN
afs-72443	147	9	identification	identification	NOUN
afs-72443	147	10	in	in	ADP
afs-72443	147	11	humans	human	NOUN
afs-72443	147	12	,	,	PUNCT
afs-72443	147	13	cattle	cattle	NOUN
afs-72443	147	14	,	,	PUNCT
afs-72443	147	15	sheep	sheep	NOUN
afs-72443	147	16	and	and	CCONJ
afs-72443	147	17	goats	goat	NOUN
afs-72443	147	18	.	.	PUNCT
afs-72443	148	1	bio	bio	PROPN
afs-72443	148	2	/	/	SYM
afs-72443	148	3	technology	technology	NOUN
afs-72443	148	4	8	8	NUM
afs-72443	148	5	:	:	SYM
afs-72443	148	6	1279	1279	NUM
afs-72443	148	7	-	-	SYM
afs-72443	148	8	1281	1281	NUM
afs-72443	148	9	.	.	PUNCT
afs-72443	149	1	ashworth	ashworth	PROPN
afs-72443	149	2	,	,	PUNCT
afs-72443	149	3	a.	a.	NOUN
afs-72443	149	4	,	,	PUNCT
afs-72443	149	5	swift	swift	ADJ
afs-72443	149	6	,	,	PUNCT
afs-72443	149	7	s.	s.	PROPN
afs-72443	149	8	&	&	CCONJ
afs-72443	149	9	affara	affara	PROPN
afs-72443	149	10	,	,	PUNCT
afs-72443	149	11	n.	n.	PROPN
afs-72443	149	12	1989	1989	NUM
afs-72443	149	13	.	.	PUNCT
afs-72443	150	1	sequence	sequence	NOUN
afs-72443	150	2	of	of	ADP
afs-72443	150	3	cdna	cdna	PROPN
afs-72443	150	4	for	for	ADP
afs-72443	150	5	murine	murine	ADJ
afs-72443	150	6	zfy-1	zfy-1	NOUN
afs-72443	150	7	,	,	PUNCT
afs-72443	150	8	a	a	DET
afs-72443	150	9	candidate	candidate	NOUN
afs-72443	150	10	for	for	ADP
afs-72443	150	11	tdy	tdy	PROPN
afs-72443	150	12	.	.	PUNCT
afs-72443	151	1	nucleic	nucleic	ADJ
afs-72443	151	2	acids	acid	NOUN
afs-72443	151	3	res	re	NOUN
afs-72443	151	4	.	.	PUNCT
afs-72443	152	1	17:2864	17:2864	NUM
afs-72443	152	2	.	.	PUNCT
afs-72443	153	1	bradbury	bradbury	PROPN
afs-72443	153	2	,	,	PUNCT
afs-72443	153	3	m.w	m.w	PROPN
afs-72443	153	4	.	.	PROPN
afs-72443	153	5	,	,	PUNCT
afs-72443	153	6	isola	isola	PROPN
afs-72443	153	7	,	,	PUNCT
afs-72443	153	8	l.m	l.m	PROPN
afs-72443	153	9	.	.	PROPN
afs-72443	153	10	,	,	PUNCT
afs-72443	153	11	&	&	CCONJ
afs-72443	153	12	gordon	gordon	PROPN
afs-72443	153	13	,	,	PUNCT
afs-72443	153	14	j.w	j.w	PROPN
afs-72443	153	15	.	.	PROPN
afs-72443	153	16	1990	1990	NUM
afs-72443	153	17	.	.	PUNCT
afs-72443	154	1	enzymatic	enzymatic	ADJ
afs-72443	154	2	amplification	amplification	NOUN
afs-72443	154	3	ofa	ofa	PROPN
afs-72443	154	4	y	y	PROPN
afs-72443	154	5	chromosome	chromosome	NOUN
afs-72443	154	6	repeat	repeat	NOUN
afs-72443	154	7	in	in	ADP
afs-72443	154	8	a	a	DET
afs-72443	154	9	single	single	ADJ
afs-72443	154	10	blastomere	blastomere	NOUN
afs-72443	154	11	allows	allow	VERB
afs-72443	154	12	identification	identification	NOUN
afs-72443	154	13	of	of	ADP
afs-72443	154	14	the	the	DET
afs-72443	154	15	sex	sex	NOUN
afs-72443	154	16	of	of	ADP
afs-72443	154	17	preimplantation	preimplantation	NOUN
afs-72443	154	18	mouse	mouse	NOUN
afs-72443	154	19	embryos	embryos	NOUN
afs-72443	154	20	.	.	PUNCT
afs-72443	155	1	proc	proc	PROPN
afs-72443	155	2	.	.	PUNCT
afs-72443	156	1	natl	natl	PROPN
afs-72443	156	2	.	.	PUNCT
afs-72443	157	1	acad	acad	PROPN
afs-72443	157	2	.	.	PUNCT
afs-72443	158	1	sci	sci	PROPN
afs-72443	158	2	.	.	PROPN
afs-72443	158	3	usa	usa	PROPN
afs-72443	158	4	,	,	PUNCT
afs-72443	158	5	86	86	NUM
afs-72443	158	6	:	:	SYM
afs-72443	158	7	4053	4053	NUM
afs-72443	158	8	-	-	SYM
afs-72443	158	9	4057	4057	NUM
afs-72443	158	10	.	.	PUNCT
afs-72443	159	1	bredbacka	bredbacka	NOUN
afs-72443	159	2	,	,	PUNCT
afs-72443	159	3	p.	p.	NOUN
afs-72443	159	4	1991	1991	NUM
afs-72443	159	5	.	.	PUNCT
afs-72443	160	1	biopsy	biopsy	NOUN
afs-72443	160	2	of	of	ADP
afs-72443	160	3	morulae	morulae	NOUN
afs-72443	160	4	and	and	CCONJ
afs-72443	160	5	blastocysts	blastocyst	NOUN
afs-72443	160	6	.	.	PUNCT
afs-72443	161	1	237	237	NUM
afs-72443	161	2	agric	agric	NOUN
afs-72443	161	3	.	.	PUNCT
afs-72443	162	1	sei	sei	ADJ
afs-72443	162	2	.	.	PUNCT
afs-72443	162	3	finl	finl	NOUN
afs-72443	162	4	.	.	PUNCT
afs-72443	163	1	1	1	NUM
afs-72443	163	2	(	(	PUNCT
afs-72443	163	3	1992	1992	NUM
afs-72443	163	4	)	)	PUNCT
afs-72443	163	5	reprod	reprod	NOUN
afs-72443	163	6	.	.	PUNCT
afs-72443	164	1	dom	dom	NOUN
afs-72443	164	2	.	.	PUNCT
afs-72443	165	1	anim	anim	NOUN
afs-72443	165	2	.	.	PUNCT
afs-72443	166	1	26	26	NUM
afs-72443	166	2	:	:	PUNCT
afs-72443	166	3	82	82	NUM
afs-72443	166	4	-	-	SYM
afs-72443	166	5	84	84	NUM
afs-72443	166	6	.	.	PUNCT
afs-72443	167	1	—	—	PUNCT
afs-72443	167	2	,	,	PUNCT
afs-72443	167	3	bredbacka	bredbacka	PROPN
afs-72443	167	4	,	,	PUNCT
afs-72443	167	5	k.	k.	PROPN
afs-72443	167	6	&	&	CCONJ
afs-72443	167	7	peippo	peippo	PROPN
afs-72443	167	8	,	,	PUNCT
afs-72443	167	9	j.	j.	PROPN
afs-72443	167	10	1991.experiences	1991.experiences	PROPN
afs-72443	167	11	of	of	ADP
afs-72443	167	12	using	use	VERB
afs-72443	167	13	pcr	pcr	NOUN
afs-72443	167	14	for	for	ADP
afs-72443	167	15	sexing	sex	VERB
afs-72443	167	16	bovine	bovine	ADJ
afs-72443	167	17	embryos	embryos	PROPN
afs-72443	167	18	.	.	PUNCT
afs-72443	168	1	reprod	reprod	PROPN
afs-72443	168	2	.	.	PUNCT
afs-72443	169	1	dom	dom	PROPN
afs-72443	169	2	.	.	PUNCT
afs-72443	170	1	anim	anim	NOUN
afs-72443	170	2	.	.	PUNCT
afs-72443	171	1	26	26	NUM
afs-72443	171	2	:	:	PUNCT
afs-72443	171	3	75	75	NUM
afs-72443	171	4	-	-	SYM
afs-72443	171	5	77	77	NUM
afs-72443	171	6	.	.	PUNCT
afs-72443	171	7	cm	cm	NOUN
afs-72443	171	8	,	,	PUNCT
afs-72443	171	9	x.	x.	PROPN
afs-72443	171	10	,	,	PUNCT
afs-72443	171	11	li	li	PROPN
afs-72443	171	12	,	,	PUNCT
afs-72443	171	13	h.	h.	PROPN
afs-72443	171	14	,	,	PUNCT
afs-72443	171	15	goradia	goradia	PROPN
afs-72443	171	16	,	,	PUNCT
afs-72443	171	17	t.m	t.m	PROPN
afs-72443	171	18	.	.	PROPN
afs-72443	171	19	,	,	PUNCT
afs-72443	171	20	lange	lange	PROPN
afs-72443	171	21	,	,	PUNCT
afs-72443	171	22	k.	k.	PROPN
afs-72443	171	23	,	,	PUNCT
afs-72443	171	24	kazazian	kazazian	PROPN
afs-72443	171	25	,	,	PUNCT
afs-72443	171	26	h.h	h.h	PROPN
afs-72443	171	27	.	.	PROPN
afs-72443	171	28	,	,	PUNCT
afs-72443	171	29	galas	gala	NOUN
afs-72443	171	30	,	,	PUNCT
afs-72443	171	31	d.	d.	PROPN
afs-72443	171	32	&	&	CCONJ
afs-72443	171	33	arnheim	arnheim	PROPN
afs-72443	171	34	,	,	PUNCT
afs-72443	171	35	n.	n.	PROPN
afs-72443	171	36	1989	1989	NUM
afs-72443	171	37	.	.	PUNCT
afs-72443	172	1	single	single	ADJ
afs-72443	172	2	-	-	PUNCT
afs-72443	172	3	sperm	sperm	NOUN
afs-72443	172	4	typing	typing	NOUN
afs-72443	172	5	:	:	PUNCT
afs-72443	172	6	determination	determination	NOUN
afs-72443	172	7	of	of	ADP
afs-72443	172	8	genetic	genetic	ADJ
afs-72443	172	9	distance	distance	NOUN
afs-72443	172	10	between	between	ADP
afs-72443	172	11	the	the	DET
afs-72443	172	12	°	°	PROPN
afs-72443	172	13	-globin	-globin	NOUN
afs-72443	172	14	and	and	CCONJ
afs-72443	172	15	parathyroid	parathyroid	ADJ
afs-72443	172	16	hormone	hormone	NOUN
afs-72443	172	17	loci	locus	NOUN
afs-72443	172	18	by	by	ADP
afs-72443	172	19	using	use	VERB
afs-72443	172	20	the	the	DET
afs-72443	172	21	polymerase	polymerase	NOUN
afs-72443	172	22	chain	chain	NOUN
afs-72443	172	23	reaction	reaction	NOUN
afs-72443	172	24	and	and	CCONJ
afs-72443	172	25	allele	allele	NOUN
afs-72443	172	26	-	-	PUNCT
afs-72443	172	27	specific	specific	ADJ
afs-72443	172	28	oligomers	oligomer	NOUN
afs-72443	172	29	.	.	PUNCT
afs-72443	173	1	proc	proc	NOUN
afs-72443	173	2	.	.	PUNCT
afs-72443	174	1	natl	natl	PROPN
afs-72443	174	2	.	.	PUNCT
afs-72443	175	1	acad	acad	PROPN
afs-72443	175	2	.	.	PUNCT
afs-72443	176	1	sci	sci	PROPN
afs-72443	176	2	.	.	PROPN
afs-72443	176	3	usa	usa	PROPN
afs-72443	176	4	.	.	PROPN
afs-72443	176	5	86	86	NUM
afs-72443	176	6	:	:	PUNCT
afs-72443	176	7	9389	9389	NUM
afs-72443	176	8	-	-	SYM
afs-72443	176	9	9393	9393	NUM
afs-72443	176	10	.	.	PUNCT
afs-72443	177	1	flandyside	flandyside	PROPN
afs-72443	177	2	,	,	PUNCT
afs-72443	177	3	a.h	a.h	PROPN
afs-72443	177	4	.	.	PROPN
afs-72443	177	5	,	,	PUNCT
afs-72443	177	6	penketh	penketh	PROPN
afs-72443	177	7	,	,	PUNCT
afs-72443	177	8	r.j.a	r.j.a	PROPN
afs-72443	177	9	.	.	PROPN
afs-72443	177	10	,	,	PUNCT
afs-72443	177	11	winston	winston	PROPN
afs-72443	177	12	,	,	PUNCT
afs-72443	177	13	r.m.l	r.m.l	PROPN
afs-72443	177	14	.	.	PROPN
afs-72443	177	15	,	,	PUNCT
afs-72443	177	16	pattinson	pattinson	PROPN
afs-72443	177	17	,	,	PUNCT
afs-72443	177	18	j.k	j.k	PROPN
afs-72443	177	19	.	.	PROPN
afs-72443	177	20	,	,	PUNCT
afs-72443	177	21	delhanty	delhanty	INTJ
afs-72443	177	22	,	,	PUNCT
afs-72443	177	23	j.d.a	j.d.a	PROPN
afs-72443	177	24	.	.	PROPN
afs-72443	177	25	&	&	CCONJ
afs-72443	177	26	tuddenham	tuddenham	PROPN
afs-72443	177	27	,	,	PUNCT
afs-72443	177	28	e.g.d	e.g.d	NOUN
afs-72443	177	29	.	.	PUNCT
afs-72443	177	30	1989	1989	NUM
afs-72443	177	31	.	.	PUNCT
afs-72443	178	1	biopsy	biopsy	NOUN
afs-72443	178	2	of	of	ADP
afs-72443	178	3	human	human	ADJ
afs-72443	178	4	preimplantation	preimplantation	NOUN
afs-72443	178	5	embryos	embryo	NOUN
afs-72443	178	6	and	and	CCONJ
afs-72443	178	7	sexing	sex	VERB
afs-72443	178	8	by	by	ADP
afs-72443	178	9	dna	dna	PROPN
afs-72443	178	10	amplification	amplification	NOUN
afs-72443	178	11	.	.	PUNCT
afs-72443	179	1	lancet	lancet	PROPN
afs-72443	179	2	ii	ii	PROPN
afs-72443	179	3	:	:	PUNCT
afs-72443	179	4	347	347	NUM
afs-72443	179	5	-	-	SYM
afs-72443	179	6	349	349	NUM
afs-72443	179	7	.	.	PUNCT
afs-72443	180	1	herr	herr	PROPN
afs-72443	180	2	,	,	PUNCT
afs-72443	180	3	c.m	c.m	PROPN
afs-72443	180	4	.	.	PROPN
afs-72443	180	5	,	,	PUNCT
afs-72443	180	6	holt	holt	PROPN
afs-72443	180	7	,	,	PUNCT
afs-72443	180	8	n.a	n.a	PROPN
afs-72443	180	9	.	.	PROPN
afs-72443	180	10	,	,	PUNCT
afs-72443	180	11	matthaei	matthaei	PROPN
afs-72443	180	12	,	,	PUNCT
afs-72443	180	13	k.i	k.i	PROPN
afs-72443	180	14	.	.	PROPN
afs-72443	180	15	&	&	CCONJ
afs-72443	180	16	reed	reed	PROPN
afs-72443	180	17	,	,	PUNCT
afs-72443	180	18	k.c	k.c	PROPN
afs-72443	180	19	.	.	PROPN
afs-72443	180	20	1990	1990	NUM
afs-72443	180	21	a.	a.	NOUN
afs-72443	180	22	sex	sex	NOUN
afs-72443	180	23	ofprogeny	ofprogeny	NOUN
afs-72443	180	24	from	from	ADP
afs-72443	180	25	bovine	bovine	ADJ
afs-72443	180	26	embryos	embryo	NOUN
afs-72443	180	27	sexed	sex	VERB
afs-72443	180	28	with	with	ADP
afs-72443	180	29	a	a	DET
afs-72443	180	30	rapid	rapid	ADJ
afs-72443	180	31	y	y	ADJ
afs-72443	180	32	-	-	PUNCT
afs-72443	180	33	chromosome	chromosome	NOUN
afs-72443	180	34	detection	detection	NOUN
afs-72443	180	35	assay	assay	VERB
afs-72443	180	36	.	.	PUNCT
afs-72443	181	1	theriogenology	theriogenology	NOUN
afs-72443	181	2	33	33	NUM
afs-72443	181	3	:	:	SYM
afs-72443	181	4	247	247	NUM
afs-72443	181	5	.	.	PUNCT
afs-72443	182	1	—	—	PUNCT
afs-72443	182	2	,	,	PUNCT
afs-72443	182	3	matthaei	matthaei	NOUN
afs-72443	182	4	,	,	PUNCT
afs-72443	182	5	k.	k.	PROPN
afs-72443	182	6	1	1	NUM
afs-72443	182	7	.	.	NUM
afs-72443	182	8	,	,	PUNCT
afs-72443	182	9	petrzak	petrzak	NOUN
afs-72443	182	10	,	,	PUNCT
afs-72443	182	11	u.	u.	PROPN
afs-72443	182	12	&	&	CCONJ
afs-72443	182	13	reed	reed	PROPN
afs-72443	182	14	,	,	PUNCT
afs-72443	182	15	k.c	k.c	PROPN
afs-72443	182	16	.	.	PROPN
afs-72443	182	17	1990b	1990b	NUM
afs-72443	182	18	.	.	PUNCT
afs-72443	183	1	a	a	DET
afs-72443	183	2	rapid	rapid	ADJ
afs-72443	183	3	y	y	ADJ
afs-72443	183	4	-	-	PUNCT
afs-72443	183	5	chromosome	chromosome	NOUN
afs-72443	183	6	-	-	PUNCT
afs-72443	183	7	detecting	detect	VERB
afs-72443	183	8	ovine	ovine	ADJ
afs-72443	183	9	embryo	embryo	NOUN
afs-72443	183	10	sexing	sexing	NOUN
afs-72443	183	11	assay	assay	NOUN
afs-72443	183	12	.	.	PUNCT
afs-72443	184	1	theriogenology	theriogenology	NOUN
afs-72443	184	2	33	33	NUM
afs-72443	184	3	:	:	SYM
afs-72443	184	4	245	245	NUM
afs-72443	184	5	.	.	PUNCT
afs-72443	184	6	page	page	NOUN
afs-72443	184	7	,	,	PUNCT
afs-72443	184	8	d.c	d.c	PROPN
afs-72443	184	9	.	.	PROPN
afs-72443	184	10	,	,	PUNCT
afs-72443	184	11	moster	moster	PROPN
afs-72443	184	12	,	,	PUNCT
afs-72443	184	13	r.	r.	PROPN
afs-72443	184	14	,	,	PUNCT
afs-72443	184	15	simpson	simpson	PROPN
afs-72443	184	16	,	,	PUNCT
afs-72443	184	17	e.m	e.m	PROPN
afs-72443	184	18	.	.	PROPN
afs-72443	184	19	,	,	PUNCT
afs-72443	184	20	fisher	fisher	PROPN
afs-72443	184	21	,	,	PUNCT
afs-72443	184	22	e.m	e.m	PROPN
afs-72443	184	23	.	.	PROPN
afs-72443	184	24	,	,	PUNCT
afs-72443	184	25	mardon	mardon	PROPN
afs-72443	184	26	,	,	PUNCT
afs-72443	184	27	g.	g.	PROPN
afs-72443	184	28	,	,	PUNCT
afs-72443	184	29	pollack	pollack	PROPN
afs-72443	184	30	,	,	PUNCT
afs-72443	184	31	j.	j.	PROPN
afs-72443	184	32	,	,	PUNCT
afs-72443	184	33	mcgilluray	mcgilluray	ADJ
afs-72443	184	34	,	,	PUNCT
afs-72443	184	35	8	8	NUM
afs-72443	184	36	.	.	NUM
afs-72443	184	37	,	,	PUNCT
afs-72443	184	38	de	de	PROPN
afs-72443	184	39	la	la	PROPN
afs-72443	184	40	chapelle	chapelle	PROPN
afs-72443	184	41	,	,	PUNCT
afs-72443	184	42	a.	a.	PROPN
afs-72443	184	43	&	&	CCONJ
afs-72443	184	44	brown	brown	PROPN
afs-72443	184	45	,	,	PUNCT
afs-72443	184	46	l.g	l.g	PROPN
afs-72443	184	47	.	.	PROPN
afs-72443	184	48	1987	1987	NUM
afs-72443	184	49	.	.	PUNCT
afs-72443	185	1	the	the	DET
afs-72443	185	2	sexdetermining	sexdetermine	VERB
afs-72443	185	3	region	region	NOUN
afs-72443	185	4	of	of	ADP
afs-72443	185	5	the	the	DET
afs-72443	185	6	human	human	PROPN
afs-72443	185	7	y	y	PROPN
afs-72443	185	8	-	-	PUNCT
afs-72443	185	9	chromosome	chromosome	NOUN
afs-72443	185	10	encodes	encode	VERB
afs-72443	185	11	a	a	DET
afs-72443	185	12	finger	finger	NOUN
afs-72443	185	13	protein	protein	NOUN
afs-72443	185	14	.	.	PUNCT
afs-72443	186	1	cell	cell	NOUN
afs-72443	186	2	51	51	NUM
afs-72443	186	3	:	:	PUNCT
afs-72443	186	4	1091	1091	NUM
afs-72443	186	5	-	-	SYM
afs-72443	186	6	1104	1104	NUM
afs-72443	186	7	.	.	PUNCT
afs-72443	187	1	peura	peura	PROPN
afs-72443	187	2	,	,	PUNCT
afs-72443	187	3	t.	t.	PROPN
afs-72443	187	4	,	,	PUNCT
afs-72443	187	5	hyttinen	hyttinen	PROPN
afs-72443	187	6	,	,	PUNCT
afs-72443	187	7	j.-m	j.-m	PROPN
afs-72443	187	8	.	.	PROPN
afs-72443	187	9	,	,	PUNCT
afs-72443	188	1	turunen	turunen	PROPN
afs-72443	188	2	,	,	PUNCT
afs-72443	188	3	m.	m.	PROPN
afs-72443	188	4	&	&	CCONJ
afs-72443	188	5	jänne	jänne	PROPN
afs-72443	188	6	,	,	PUNCT
afs-72443	188	7	j.	j.	PROPN
afs-72443	188	8	1991	1991	NUM
afs-72443	188	9	.	.	PUNCT
afs-72443	189	1	a	a	DET
afs-72443	189	2	reliable	reliable	ADJ
afs-72443	189	3	sex	sex	NOUN
afs-72443	189	4	determination	determination	NOUN
afs-72443	189	5	assay	assay	VERB
afs-72443	189	6	for	for	ADP
afs-72443	189	7	bovine	bovine	ADJ
afs-72443	189	8	preimplantation	preimplantation	NOUN
afs-72443	189	9	embryos	embryo	NOUN
afs-72443	189	10	using	use	VERB
afs-72443	189	11	the	the	DET
afs-72443	189	12	polymerase	polymerase	NOUN
afs-72443	189	13	chain	chain	NOUN
afs-72443	189	14	reaction	reaction	NOUN
afs-72443	189	15	.	.	PUNCT
afs-72443	190	1	theriogenology	theriogenology	NOUN
afs-72443	190	2	35	35	NUM
afs-72443	190	3	:	:	PUNCT
afs-72443	190	4	547	547	NUM
afs-72443	190	5	-	-	SYM
afs-72443	190	6	555	555	NUM
afs-72443	190	7	,	,	PUNCT
afs-72443	190	8	saiki	saiki	ADJ
afs-72443	190	9	,	,	PUNCT
afs-72443	190	10	r.k	r.k	PROPN
afs-72443	190	11	.	.	PROPN
afs-72443	190	12	,	,	PUNCT
afs-72443	190	13	scharf	scharf	PROPN
afs-72443	190	14	,	,	PUNCT
afs-72443	190	15	s.	s.	PROPN
afs-72443	190	16	,	,	PUNCT
afs-72443	190	17	faloona	faloona	PROPN
afs-72443	190	18	,	,	PUNCT
afs-72443	190	19	f.	f.	PROPN
afs-72443	190	20	,	,	PUNCT
afs-72443	190	21	muixis	muixis	ADJ
afs-72443	190	22	,	,	PUNCT
afs-72443	190	23	k	k	PROPN
afs-72443	190	24	8	8	NUM
afs-72443	190	25	.	.	PUNCT
afs-72443	190	26	,	,	PUNCT
afs-72443	190	27	horn	horn	PROPN
afs-72443	190	28	,	,	PUNCT
afs-72443	190	29	g.t	g.t	PROPN
afs-72443	190	30	.	.	PROPN
afs-72443	190	31	,	,	PUNCT
afs-72443	190	32	erlich	erlich	PROPN
afs-72443	190	33	,	,	PUNCT
afs-72443	190	34	h.a	h.a	PROPN
afs-72443	190	35	.	.	PROPN
afs-72443	190	36	&	&	CCONJ
afs-72443	190	37	arnheim	arnheim	PROPN
afs-72443	190	38	,	,	PUNCT
afs-72443	190	39	n.	n.	NOUN
afs-72443	190	40	1985	1985	NUM
afs-72443	190	41	.	.	PUNCT
afs-72443	191	1	enzymatic	enzymatic	ADJ
afs-72443	191	2	amplification	amplification	NOUN
afs-72443	191	3	of	of	ADP
afs-72443	191	4	b	b	NOUN
afs-72443	191	5	-	-	PUNCT
afs-72443	191	6	globin	globin	ADJ
afs-72443	191	7	genomic	genomic	NOUN
afs-72443	191	8	sequences	sequence	NOUN
afs-72443	191	9	and	and	CCONJ
afs-72443	191	10	restriction	restriction	NOUN
afs-72443	191	11	site	site	NOUN
afs-72443	191	12	analysis	analysis	NOUN
afs-72443	191	13	for	for	ADP
afs-72443	191	14	diagnosis	diagnosis	NOUN
afs-72443	191	15	of	of	ADP
afs-72443	191	16	sickle	sickle	NOUN
afs-72443	191	17	cell	cell	NOUN
afs-72443	191	18	anemia	anemia	NOUN
afs-72443	191	19	.	.	PUNCT
afs-72443	192	1	science	science	NOUN
afs-72443	192	2	230	230	NUM
afs-72443	192	3	:	:	PUNCT
afs-72443	192	4	1350	1350	NUM
afs-72443	192	5	-	-	SYM
afs-72443	192	6	1354	1354	NUM
afs-72443	192	7	.	.	PUNCT
afs-72443	193	1	—	—	PUNCT
afs-72443	193	2	,	,	PUNCT
afs-72443	193	3	gelfand	gelfand	PROPN
afs-72443	193	4	,	,	PUNCT
afs-72443	193	5	d.h	d.h	PROPN
afs-72443	193	6	.	.	PROPN
afs-72443	193	7	,	,	PUNCT
afs-72443	193	8	stoffel	stoffel	PROPN
afs-72443	193	9	,	,	PUNCT
afs-72443	193	10	s.	s.	PROPN
afs-72443	193	11	,	,	PUNCT
afs-72443	193	12	scharf	scharf	PROPN
afs-72443	193	13	,	,	PUNCT
afs-72443	193	14	s.j	s.j	PROPN
afs-72443	193	15	.	.	PROPN
afs-72443	193	16	,	,	PUNCT
afs-72443	193	17	higuchi	higuchi	PROPN
afs-72443	193	18	,	,	PUNCT
afs-72443	193	19	r.	r.	PROPN
afs-72443	193	20	,	,	PUNCT
afs-72443	193	21	horn	horn	PROPN
afs-72443	193	22	,	,	PUNCT
afs-72443	193	23	g.t	g.t	PROPN
afs-72443	193	24	.	.	PROPN
afs-72443	193	25	,	,	PUNCT
afs-72443	193	26	mullis	mullis	PROPN
afs-72443	193	27	,	,	PUNCT
afs-72443	194	1	k.b	k.b	PROPN
afs-72443	194	2	.	.	PROPN
afs-72443	194	3	&	&	CCONJ
afs-72443	194	4	erlich	erlich	PROPN
afs-72443	194	5	,	,	PUNCT
afs-72443	194	6	h.a	h.a	PROPN
afs-72443	194	7	,	,	PUNCT
afs-72443	194	8	1988	1988	NUM
afs-72443	194	9	.	.	PUNCT
afs-72443	195	1	primer	primer	NOUN
afs-72443	195	2	-	-	PUNCT
afs-72443	195	3	directed	direct	VERB
afs-72443	195	4	enzymatic	enzymatic	ADJ
afs-72443	195	5	amplification	amplification	NOUN
afs-72443	195	6	ofdna	ofdna	NOUN
afs-72443	195	7	with	with	ADP
afs-72443	195	8	a	a	DET
afs-72443	195	9	thermostable	thermostable	ADJ
afs-72443	195	10	dna	dna	NOUN
afs-72443	195	11	polymerase	polymerase	NOUN
afs-72443	195	12	.	.	PUNCT
afs-72443	196	1	science	science	NOUN
afs-72443	196	2	239	239	NUM
afs-72443	196	3	:	:	PUNCT
afs-72443	196	4	487	487	NUM
afs-72443	196	5	-	-	SYM
afs-72443	196	6	491	491	NUM
afs-72443	196	7	.	.	PUNCT
afs-72443	197	1	schröder	schröder	NOUN
afs-72443	197	2	,	,	PUNCT
afs-72443	197	3	a.	a.	NOUN
afs-72443	197	4	,	,	PUNCT
afs-72443	197	5	miller	miller	PROPN
afs-72443	197	6	,	,	PUNCT
afs-72443	197	7	j.r	j.r	PROPN
afs-72443	197	8	.	.	PROPN
afs-72443	197	9	,	,	PUNCT
afs-72443	197	10	thomsen	thomsen	PROPN
afs-72443	197	11	,	,	PUNCT
afs-72443	197	12	p.d	p.d	PROPN
afs-72443	197	13	.	.	PROPN
afs-72443	197	14	,	,	PUNCT
afs-72443	197	15	roschlau	roschlau	PROPN
afs-72443	197	16	,	,	PUNCT
afs-72443	197	17	k.	k.	PROPN
afs-72443	197	18	,	,	PUNCT
afs-72443	197	19	avery	avery	PROPN
afs-72443	197	20	,	,	PUNCT
afs-72443	197	21	8	8	NUM
afs-72443	197	22	.	.	NUM
afs-72443	197	23	,	,	PUNCT
afs-72443	197	24	poulsen	poulsen	VERB
afs-72443	197	25	,	,	PUNCT
afs-72443	197	26	p.	p.	NOUN
afs-72443	197	27	,	,	PUNCT
afs-72443	197	28	schmidt	schmidt	PROPN
afs-72443	197	29	,	,	PUNCT
afs-72443	197	30	m.	m.	NOUN
afs-72443	197	31	&	&	CCONJ
afs-72443	197	32	schwerin	schwerin	NOUN
afs-72443	197	33	,	,	PUNCT
afs-72443	197	34	m.	m.	NOUN
afs-72443	197	35	1990	1990	NUM
afs-72443	197	36	.	.	PUNCT
afs-72443	198	1	sex	sex	NOUN
afs-72443	198	2	determination	determination	NOUN
afs-72443	198	3	of	of	ADP
afs-72443	198	4	bovine	bovine	ADJ
afs-72443	198	5	embryos	embryo	NOUN
afs-72443	198	6	using	use	VERB
afs-72443	198	7	the	the	DET
afs-72443	198	8	polymerase	polymerase	NOUN
afs-72443	198	9	chain	chain	NOUN
afs-72443	198	10	reaction	reaction	NOUN
afs-72443	198	11	.	.	PUNCT
afs-72443	199	1	animal	animal	NOUN
afs-72443	199	2	biotechnology	biotechnology	NOUN
afs-72443	199	3	i	i	NOUN
afs-72443	199	4	:	:	PUNCT
afs-72443	199	5	121	121	NUM
afs-72443	199	6	-	-	SYM
afs-72443	199	7	133	133	NUM
afs-72443	199	8	.	.	PUNCT
afs-72443	200	1	setiabudi	setiabudi	PROPN
afs-72443	200	2	,	,	PUNCT
afs-72443	200	3	r.	r.	PROPN
afs-72443	200	4	&	&	CCONJ
afs-72443	200	5	gustavsson	gustavsson	PROPN
afs-72443	200	6	,	,	PUNCT
afs-72443	200	7	i.	i.	PROPN
afs-72443	200	8	1991	1991	NUM
afs-72443	200	9	.	.	PUNCT
afs-72443	201	1	establishment	establishment	NOUN
afs-72443	201	2	of	of	ADP
afs-72443	201	3	embryo	embryo	NOUN
afs-72443	201	4	sexing	sex	VERB
afs-72443	201	5	techniques	technique	NOUN
afs-72443	201	6	in	in	ADP
afs-72443	201	7	sweden	sweden	PROPN
afs-72443	201	8	.	.	PUNCT
afs-72443	202	1	reprod	reprod	PROPN
afs-72443	202	2	.	.	PUNCT
afs-72443	203	1	dom	dom	PROPN
afs-72443	203	2	.	.	PUNCT
afs-72443	203	3	anim	anim	PROPN
afs-72443	203	4	,	,	PUNCT
afs-72443	203	5	26	26	NUM
afs-72443	203	6	:	:	SYM
afs-72443	203	7	78	78	NUM
afs-72443	203	8	-	-	SYM
afs-72443	203	9	81	81	NUM
afs-72443	203	10	.	.	PUNCT
afs-72443	204	1	van	van	PROPN
afs-72443	204	2	vliet	vliet	PROPN
afs-72443	204	3	,	,	PUNCT
afs-72443	204	4	r.a	r.a	PROPN
afs-72443	204	5	.	.	PROPN
afs-72443	204	6	,	,	PUNCT
afs-72443	204	7	verringer	verringer	NOUN
afs-72443	204	8	gibbins	gibbin	NOUN
afs-72443	204	9	,	,	PUNCT
afs-72443	204	10	a.m.	a.m.	PROPN
afs-72443	204	11	&	&	CCONJ
afs-72443	204	12	walton	walton	PROPN
afs-72443	204	13	,	,	PUNCT
afs-72443	204	14	j.s	j.s	PROPN
afs-72443	204	15	.	.	PROPN
afs-72443	205	1	1989.livestock	1989.livestock	ADJ
afs-72443	205	2	embryo	embryo	NOUN
afs-72443	205	3	sexing	sexing	NOUN
afs-72443	205	4	:	:	PUNCT
afs-72443	205	5	a	a	DET
afs-72443	205	6	review	review	NOUN
afs-72443	205	7	ofcurrent	ofcurrent	NOUN
afs-72443	205	8	methods	method	NOUN
afs-72443	205	9	with	with	ADP
afs-72443	205	10	emphasis	emphasis	NOUN
afs-72443	205	11	on	on	ADP
afs-72443	205	12	y	y	NOUN
afs-72443	205	13	-	-	PUNCT
afs-72443	205	14	specific	specific	ADJ
afs-72443	205	15	dna	dna	NOUN
afs-72443	205	16	probes	probe	NOUN
afs-72443	205	17	.	.	PUNCT
afs-72443	206	1	theriogenology	theriogenology	NOUN
afs-72443	206	2	32	32	NUM
afs-72443	206	3	:	:	PUNCT
afs-72443	206	4	421	421	NUM
afs-72443	206	5	-	-	SYM
afs-72443	206	6	438	438	NUM
afs-72443	206	7	.	.	PUNCT
afs-72443	207	1	manuscript	manuscript	NOUN
afs-72443	207	2	received	receive	VERB
afs-72443	207	3	january	january	PROPN
afs-72443	207	4	1992	1992	NUM
afs-72443	207	5	peter	peter	PROPN
afs-72443	207	6	bredbacka	bredbacka	PROPN
afs-72443	207	7	jaana	jaana	PROPN
afs-72443	207	8	peippo	peippo	VERB
afs-72443	207	9	agricultural	agricultural	ADJ
afs-72443	207	10	research	research	NOUN
afs-72443	207	11	centre	centre	PROPN
afs-72443	207	12	of	of	ADP
afs-72443	207	13	finland	finland	PROPN
afs-72443	207	14	department	department	PROPN
afs-72443	207	15	of	of	ADP
afs-72443	207	16	animal	animal	NOUN
afs-72443	207	17	breeding	breeding	NOUN
afs-72443	207	18	sf-31600	sf-31600	PROPN
afs-72443	207	19	jokioinen	jokioinen	PROPN
afs-72443	207	20	,	,	PUNCT
afs-72443	207	21	finland	finland	PROPN
afs-72443	207	22	selostus	selostus	PROPN
afs-72443	207	23	lampaan	lampaan	PROPN
afs-72443	207	24	ja	ja	PROPN
afs-72443	207	25	naudan	naudan	PROPN
afs-72443	207	26	alkioiden	alkioiden	PROPN
afs-72443	207	27	sukupuolilajittelu	sukupuolilajittelu	PROPN
afs-72443	207	28	zfy	zfy	PROPN
afs-72443	207	29	/	/	SYM
afs-72443	207	30	zfx	zfx	NOUN
afs-72443	207	31	-	-	PUNCT
afs-72443	207	32	lokuksen	lokuksen	ADJ
afs-72443	207	33	dna	dna	NOUN
afs-72443	207	34	:	:	PUNCT
afs-72443	207	35	n	n	ADP
afs-72443	207	36	entsymaattisella	entsymaattisella	NOUN
afs-72443	207	37	monistuksella	monistuksella	PROPN
afs-72443	207	38	peter	peter	PROPN
afs-72443	207	39	bredbacka	bredbacka	PROPN
afs-72443	207	40	ja	ja	PROPN
afs-72443	207	41	jaana	jaana	PROPN
afs-72443	207	42	peippo	peippo	VERB
afs-72443	207	43	maatalouden	maatalouden	PROPN
afs-72443	207	44	tutkimuskeskus	tutkimuskesku	NOUN
afs-72443	207	45	lähiaikoina	lähiaikoina	NOUN
afs-72443	207	46	on	on	ADP
afs-72443	207	47	mahdollista	mahdollista	PROPN
afs-72443	207	48	saada	saada	PROPN
afs-72443	207	49	haluttua	haluttua	PROPN
afs-72443	208	1	sukupuolta	sukupuolta	PROPN
afs-72443	208	2	olevia	olevia	PROPN
afs-72443	208	3	jälkeläisiä	jälkeläisiä	PROPN
afs-72443	208	4	käytännön	käytännön	PROPN
afs-72443	208	5	kotieläintuotannossa	kotieläintuotannossa	PROPN
afs-72443	208	6	.	.	PUNCT
afs-72443	209	1	koska	koska	PROPN
afs-72443	209	2	siittiöiden	siittiöiden	PROPN
afs-72443	209	3	lajittelu	lajittelu	PROPN
afs-72443	209	4	xja	xja	PROPN
afs-72443	210	1	y	y	PROPN
afs-72443	210	2	-siittiöihin	-siittiöihin	PROPN
afs-72443	210	3	ennen	ennen	PROPN
afs-72443	210	4	keinosiemennystä	keinosiemennystä	PROPN
afs-72443	210	5	ei	ei	PROPN
afs-72443	210	6	vielä	vielä	PROPN
afs-72443	210	7	ole	ole	PROPN
afs-72443	210	8	tuonut	tuonut	PROPN
afs-72443	210	9	toivottavaa	toivottavaa	VERB
afs-72443	210	10	tulosta	tulosta	ADJ
afs-72443	210	11	,	,	PUNCT
afs-72443	210	12	on	on	ADP
afs-72443	210	13	ainoa	ainoa	PROPN
afs-72443	210	14	vaihtoehto	vaihtoehto	PROPN
afs-72443	210	15	tehdä	tehdä	PROPN
afs-72443	210	16	määritys	määritys	PROPN
afs-72443	210	17	suoraan	suoraan	PROPN
afs-72443	210	18	alkiosta	alkiosta	PROPN
afs-72443	210	19	ennen	ennen	PROPN
afs-72443	210	20	alkionsiirtoa	alkionsiirtoa	PROPN
afs-72443	210	21	.	.	PUNCT
afs-72443	211	1	alkiosta	alkiosta	PROPN
afs-72443	211	2	otetaan	otetaan	PROPN
afs-72443	211	3	näytepala	näytepala	PROPN
afs-72443	211	4	,	,	PUNCT
afs-72443	211	5	jonka	jonka	PROPN
afs-72443	211	6	dna	dna	PROPN
afs-72443	211	7	:	:	PUNCT
afs-72443	211	8	sta	sta	PROPN
afs-72443	211	9	määritys	määritys	PROPN
afs-72443	211	10	voidaan	voidaan	PROPN
afs-72443	211	11	tehdä	tehdä	PROPN
afs-72443	211	12	.	.	PUNCT
afs-72443	212	1	sukupuolilajittelu	sukupuolilajittelu	PROPN
afs-72443	212	2	tehdään	tehdään	PROPN
afs-72443	212	3	usein	usein	PROPN
afs-72443	212	4	dna	dna	NOUN
afs-72443	212	5	:	:	PUNCT
afs-72443	212	6	n	n	ADP
afs-72443	212	7	spesifisellä	spesifisellä	NOUN
afs-72443	212	8	monistuksella	monistuksella	NOUN
afs-72443	212	9	(	(	PUNCT
afs-72443	212	10	pcr	pcr	NOUN
afs-72443	212	11	)	)	PUNCT
afs-72443	212	12	käyttämällä	käyttämällä	PROPN
afs-72443	212	13	y	y	NOUN
afs-72443	212	14	-	-	PUNCT
afs-72443	212	15	kromosomispesifisten	kromosomispesifisten	VERB
afs-72443	212	16	alukkeiden	alukkeiden	ADJ
afs-72443	212	17	lisäksi	lisäksi	PROPN
afs-72443	212	18	autosomaalisia	autosomaalisia	PROPN
afs-72443	212	19	alukkeita	alukkeita	PROPN
afs-72443	212	20	,	,	PUNCT
afs-72443	212	21	jotka	jotka	PROPN
afs-72443	212	22	vähentävät	vähentävät	NOUN
afs-72443	212	23	virhemääritysten	virhemääritysten	VERB
afs-72443	212	24	todennäköisyyttä	todennäköisyyttä	NOUN
afs-72443	212	25	.	.	PUNCT
afs-72443	213	1	tällöin	tällöin	VERB
afs-72443	213	2	reaktio	reaktio	NOUN
afs-72443	213	3	-	-	PUNCT
afs-72443	213	4	olosuhteiden	olosuhteiden	NOUN
afs-72443	213	5	optimoiminen	optimoiminen	PROPN
afs-72443	213	6	muodostuu	muodostuu	PROPN
afs-72443	213	7	hankalaksi	hankalaksi	NOUN
afs-72443	213	8	.	.	PUNCT
afs-72443	214	1	lisäksi	lisäksi	PROPN
afs-72443	214	2	käytetyt	käytetyt	PROPN
afs-72443	214	3	alukkeet	alukkeet	PROPN
afs-72443	214	4	ovat	ovat	PROPN
afs-72443	214	5	yleensä	yleensä	PROPN
afs-72443	214	6	lajispesifisiä	lajispesifisiä	PROPN
afs-72443	214	7	,	,	PUNCT
afs-72443	214	8	minkä	minkä	PROPN
afs-72443	214	9	johdosta	johdosta	PROPN
afs-72443	214	10	optimointi	optimointi	PROPN
afs-72443	214	11	onnistuu	onnistuu	VERB
afs-72443	214	12	vain	vain	ADJ
afs-72443	214	13	käyttämällä	käyttämällä	PROPN
afs-72443	214	14	kyseisen	kyseisen	PROPN
afs-72443	214	15	lajin	lajin	PROPN
afs-72443	214	16	dna	dna	PROPN
afs-72443	214	17	:	:	PUNCT
afs-72443	214	18	ta	ta	PROPN
afs-72443	214	19	.	.	PUNCT
afs-72443	215	1	tässä	tässä	VERB
afs-72443	215	2	tutkimuksessa	tutkimuksessa	ADJ
afs-72443	215	3	nämä	nämä	PROPN
afs-72443	215	4	ongelmat	ongelmat	NOUN
afs-72443	215	5	ovat	ovat	X
afs-72443	215	6	poistettu	poistettu	VERB
afs-72443	215	7	monistamalla	monistamalla	NOUN
afs-72443	215	8	evoluutiossa	evoluutiossa	VERB
afs-72443	215	9	konservoitunutta	konservoitunutta	ADJ
afs-72443	215	10	zfy	zfy	PROPN
afs-72443	215	11	-	-	PUNCT
afs-72443	215	12	geeniä	geeniä	NOUN
afs-72443	215	13	.	.	PUNCT
afs-72443	216	1	zfy	zfy	NOUN
afs-72443	216	2	-	-	PUNCT
afs-72443	216	3	geeni	geeni	NOUN
afs-72443	216	4	on	on	ADP
afs-72443	216	5	kaikkien	kaikkien	NOUN
afs-72443	216	6	istukallisten	istukallisten	VERB
afs-72443	216	7	nisäkkäiden	nisäkkäiden	VERB
afs-72443	216	8	y	y	PROPN
afs-72443	216	9	-	-	PUNCT
afs-72443	216	10	kromosomissa	kromosomissa	PROPN
afs-72443	216	11	,	,	PUNCT
afs-72443	216	12	ja	ja	PROPN
afs-72443	216	13	sillä	sillä	VERB
afs-72443	216	14	on	on	ADP
afs-72443	216	15	homologi	homologi	PROPN
afs-72443	216	16	x	x	NOUN
afs-72443	216	17	-	-	NOUN
afs-72443	216	18	kromosomissa	kromosomissa	ADJ
afs-72443	216	19	.	.	PUNCT
afs-72443	217	1	eri	eri	PROPN
afs-72443	217	2	lajien	lajien	PROPN
afs-72443	217	3	sisällä	sisällä	PROPN
afs-72443	217	4	sukupuolten	sukupuolten	VERB
afs-72443	217	5	erottaminen	erottaminen	PROPN
afs-72443	217	6	perustuu	perustuu	VERB
afs-72443	217	7	zfy	zfy	PROPN
afs-72443	217	8	-	-	PUNCT
afs-72443	217	9	geenin	geenin	PROPN
afs-72443	217	10	sisällä	sisällä	NOUN
afs-72443	217	11	olevien	olevien	NOUN
afs-72443	217	12	,	,	PUNCT
afs-72443	217	13	lajispesifisten	lajispesifisten	VERB
afs-72443	217	14	pistemutaatioiden	pistemutaatioiden	ADJ
afs-72443	217	15	tunnistamiseen	tunnistamiseen	NOUN
afs-72443	217	16	restriktio	restriktio	NOUN
afs-72443	217	17	-	-	PUNCT
afs-72443	217	18	entsyymien	entsyymien	NOUN
afs-72443	217	19	avulla	avulla	NOUN
afs-72443	217	20	.	.	PUNCT
afs-72443	218	1	entsyymi	entsyymi	NOUN
afs-72443	218	2	-	-	PUNCT
afs-72443	218	3	käsittelyn	käsittelyn	NOUN
afs-72443	218	4	jälkeen	jälkeen	PROPN
afs-72443	218	5	saadaan	saadaan	PROPN
afs-72443	218	6	kunkin	kunkin	PROPN
afs-72443	218	7	lajin	lajin	PROPN
afs-72443	218	8	naaraille	naaraille	PROPN
afs-72443	218	9	jakoiraille	jakoiraille	PROPN
afs-72443	218	10	tunnusomaiset	tunnusomaiset	VERB
afs-72443	218	11	juovastot	juovastot	PROPN
afs-72443	218	12	geelielektroforeesissa	geelielektroforeesissa	PROPN
afs-72443	218	13	.	.	PUNCT
afs-72443	219	1	menetelmä	menetelmä	PROPN
afs-72443	219	2	optimoitiin	optimoitiin	PROPN
afs-72443	219	3	hiiren	hiiren	PROPN
afs-72443	219	4	dna	dna	PROPN
afs-72443	219	5	:	:	PUNCT
afs-72443	219	6	lla	lla	PROPN
afs-72443	219	7	,	,	PUNCT
afs-72443	219	8	ja	ja	PROPN
afs-72443	219	9	sen	sen	PROPN
afs-72443	219	10	tarkkuus	tarkkuus	PROPN
afs-72443	219	11	testattiin	testattiin	PROPN
afs-72443	219	12	pilkkomalla	pilkkomalla	PROPN
afs-72443	219	13	lampaan	lampaan	PROPN
afs-72443	219	14	alkioita	alkioita	PROPN
afs-72443	219	15	2	2	NUM
afs-72443	219	16	-	-	SYM
afs-72443	219	17	4	4	NUM
afs-72443	219	18	osaan	osaan	NOUN
afs-72443	219	19	sekä	sekä	PROPN
afs-72443	219	20	kenttäolosuhteissa	kenttäolosuhteissa	PROPN
afs-72443	219	21	että	että	PROPN
afs-72443	219	22	laboratoriossa	laboratoriossa	PROPN
afs-72443	219	23	.	.	PUNCT
afs-72443	220	1	kustakin	kustakin	PROPN
afs-72443	220	2	osasta	osasta	PROPN
afs-72443	220	3	tehtiin	tehtiin	NOUN
afs-72443	220	4	määritys	määritys	VERB
afs-72443	220	5	erikseen	erikseen	VERB
afs-72443	220	6	sokkotestinä	sokkotestinä	NOUN
afs-72443	220	7	.	.	PUNCT
afs-72443	221	1	samalla	samalla	PROPN
afs-72443	221	2	verrattiin	verrattiin	PROPN
afs-72443	221	3	erilaisia	erilaisia	PROPN
afs-72443	221	4	näytteen	näytteen	PROPN
afs-72443	221	5	käsittelytapoja	käsittelytapoja	PROPN
afs-72443	221	6	parhaan	parhaan	PROPN
afs-72443	221	7	tehokkuuden	tehokkuuden	PROPN
afs-72443	221	8	löytämiseksi	löytämiseksi	PROPN
afs-72443	221	9	.	.	PUNCT
afs-72443	222	1	joissakin	joissakin	PROPN
afs-72443	222	2	tapauksissa	tapauksissa	PROPN
afs-72443	222	3	solunäytteen	solunäytteen	VERB
afs-72443	222	4	entsymaattinen	entsymaattinen	PROPN
afs-72443	222	5	hajotus	hajotus	PROPN
afs-72443	222	6	korvattiin	korvattiin	PROPN
afs-72443	222	7	nopeammalla	nopeammalla	PROPN
afs-72443	222	8	menetelmällä	menetelmällä	PROPN
afs-72443	222	9	,	,	PUNCT
afs-72443	222	10	jossa	jossa	PROPN
afs-72443	222	11	näyte	näyte	PROPN
afs-72443	222	12	kuumennettiin	kuumennettiin	PROPN
afs-72443	222	13	emäksisessä	emäksisessä	PROPN
afs-72443	222	14	liuoksessa	liuoksessa	NOUN
afs-72443	222	15	.	.	PUNCT
afs-72443	223	1	käytetyllä	käytetyllä	PROPN
afs-72443	223	2	tekniikalla	tekniikalla	PROPN
afs-72443	223	3	määritettiin	määritettiin	NOUN
afs-72443	223	4	sukupuoli	sukupuoli	VERB
afs-72443	223	5	erittäin	erittäin	PROPN
afs-72443	223	6	suurella	suurella	PROPN
afs-72443	223	7	tarkkuudella	tarkkuudella	PROPN
afs-72443	223	8	lampaan	lampaan	PROPN
afs-72443	223	9	näytepaloista	näytepaloista	PROPN
afs-72443	223	10	,	,	PUNCT
afs-72443	223	11	jopa	jopa	PROPN
afs-72443	223	12	yhdestä	yhdestä	PROPN
afs-72443	223	13	solusta	solusta	NOUN
afs-72443	223	14	,	,	PUNCT
afs-72443	223	15	sekä	sekä	NOUN
afs-72443	223	16	naudan	naudan	PROPN
afs-72443	223	17	1	1	NUM
afs-72443	223	18	-	-	SYM
afs-72443	223	19	8	8	NUM
afs-72443	223	20	soluisista	soluisista	ADJ
afs-72443	223	21	alkioista	alkioista	NOUN
afs-72443	223	22	.	.	PUNCT
afs-72443	224	1	238	238	NUM
afs-72443	224	2	agric	agric	NOUN
afs-72443	224	3	.	.	PUNCT
afs-72443	225	1	sei	sei	ADJ
afs-72443	225	2	.	.	PUNCT
afs-72443	225	3	finl	finl	NOUN
afs-72443	225	4	.	.	PUNCT
afs-72443	226	1	1	1	NUM
afs-72443	226	2	(	(	PUNCT
afs-72443	226	3	1992	1992	NUM
afs-72443	226	4	)	)	PUNCT