id	sid	tid	token	lemma	pos
afs-72695	1	1	time	time	NOUN
afs-72695	1	2	saving	save	VERB
afs-72695	1	3	method	method	NOUN
afs-72695	1	4	for	for	ADP
afs-72695	1	5	protoplast	protoplast	NOUN
afs-72695	1	6	isolation	isolation	NOUN
afs-72695	1	7	,	,	PUNCT
afs-72695	1	8	transformation	transformation	NOUN
afs-72695	1	9	and	and	CCONJ
afs-72695	1	10	transient	transient	ADJ
afs-72695	1	11	gene	gene	NOUN
afs-72695	1	12	expression	expression	NOUN
afs-72695	1	13	assay	assay	VERB
afs-72695	1	14	in	in	ADP
afs-72695	1	15	barley	barley	NOUN
afs-72695	1	16	barnabäs	barnabäs	NOUN
afs-72695	1	17	jenes	jene	NOUN
afs-72695	1	18	,	,	PUNCT
afs-72695	1	19	matti	matti	PROPN
afs-72695	1	20	puolimatka	puolimatka	PROPN
afs-72695	1	21	,	,	PUNCT
afs-72695	1	22	pedro	pedro	PROPN
afs-72695	1	23	bittencourt	bittencourt	PROPN
afs-72695	1	24	and	and	CCONJ
afs-72695	1	25	seppo	seppo	PROPN
afs-72695	1	26	pulli	pulli	PROPN
afs-72695	1	27	jenes	jenes	PROPN
afs-72695	1	28	,	,	PUNCT
afs-72695	1	29	b.	b.	PROPN
afs-72695	1	30	'	'	PROPN
afs-72695	1	31	,	,	PUNCT
afs-72695	1	32	puolimatka	puolimatka	PROPN
afs-72695	1	33	,	,	PUNCT
afs-72695	1	34	m.	m.	NOUN
afs-72695	1	35	2	2	NUM
afs-72695	1	36	,	,	PUNCT
afs-72695	1	37	bittencourt	bittencourt	NOUN
afs-72695	1	38	,	,	PUNCT
afs-72695	1	39	p.	p.	NOUN
afs-72695	1	40	1	1	NUM
afs-72695	1	41	&	&	CCONJ
afs-72695	1	42	pulli	pulli	PROPN
afs-72695	1	43	,	,	PUNCT
afs-72695	1	44	s.	s.	PROPN
afs-72695	1	45	2	2	NUM
afs-72695	1	46	1994	1994	NUM
afs-72695	1	47	.	.	PUNCT
afs-72695	2	1	time	time	NOUN
afs-72695	2	2	saving	saving	NOUN
afs-72695	2	3	method	method	NOUN
afs-72695	2	4	for	for	ADP
afs-72695	2	5	protoplast	protoplast	NOUN
afs-72695	2	6	isolation	isolation	NOUN
afs-72695	2	7	,	,	PUNCT
afs-72695	2	8	transformation	transformation	NOUN
afs-72695	2	9	and	and	CCONJ
afs-72695	2	10	transient	transient	ADJ
afs-72695	2	11	gene	gene	NOUN
afs-72695	2	12	expression	expression	NOUN
afs-72695	2	13	assay	assay	VERB
afs-72695	2	14	in	in	ADP
afs-72695	2	15	barley	barley	NOUN
afs-72695	2	16	.	.	PUNCT
afs-72695	3	1	agricultural	agricultural	ADJ
afs-72695	3	2	science	science	NOUN
afs-72695	3	3	in	in	ADP
afs-72695	3	4	finland	finland	PROPN
afs-72695	3	5	3	3	NUM
afs-72695	3	6	:	:	SYM
afs-72695	3	7	199	199	NUM
afs-72695	3	8	-	-	SYM
afs-72695	3	9	205	205	NUM
afs-72695	3	10	.	.	PUNCT
afs-72695	4	1	(	(	PUNCT
afs-72695	4	2	‘	'	PUNCT
afs-72695	4	3	lnstitute	lnstitute	VERB
afs-72695	4	4	for	for	ADP
afs-72695	4	5	plant	plant	NOUN
afs-72695	4	6	sciences	science	NOUN
afs-72695	4	7	,	,	PUNCT
afs-72695	4	8	agricultural	agricultural	ADJ
afs-72695	4	9	biotechnology	biotechnology	NOUN
afs-72695	4	10	center	center	NOUN
afs-72695	4	11	,	,	PUNCT
afs-72695	4	12	gödöllö	gödöllö	NOUN
afs-72695	4	13	,	,	PUNCT
afs-72695	4	14	p.0.80x	p.0.80x	NOUN
afs-72695	4	15	170	170	NUM
afs-72695	4	16	,	,	PUNCT
afs-72695	4	17	h-2100	h-2100	VERB
afs-72695	4	18	hungary	hungary	PROPN
afs-72695	4	19	and	and	CCONJ
afs-72695	4	20	agricultural	agricultural	ADJ
afs-72695	4	21	research	research	NOUN
afs-72695	4	22	centre	centre	NOUN
afs-72695	4	23	of	of	ADP
afs-72695	4	24	finland	finland	PROPN
afs-72695	4	25	,	,	PUNCT
afs-72695	4	26	institute	institute	NOUN
afs-72695	4	27	of	of	ADP
afs-72695	4	28	crop	crop	NOUN
afs-72695	4	29	and	and	CCONJ
afs-72695	4	30	soil	soil	NOUN
afs-72695	4	31	science	science	NOUN
afs-72695	4	32	,	,	PUNCT
afs-72695	4	33	plant	plant	NOUN
afs-72695	4	34	breeding	breeding	NOUN
afs-72695	4	35	section	section	NOUN
afs-72695	4	36	,	,	PUNCT
afs-72695	4	37	fin-31600	fin-31600	PROPN
afs-72695	4	38	jokioinen	jokioinen	PROPN
afs-72695	4	39	,	,	PUNCT
afs-72695	4	40	finland	finland	PROPN
afs-72695	4	41	.	.	PUNCT
afs-72695	4	42	)	)	PUNCT
afs-72695	5	1	this	this	DET
afs-72695	5	2	study	study	NOUN
afs-72695	5	3	was	be	AUX
afs-72695	5	4	conducted	conduct	VERB
afs-72695	5	5	to	to	PART
afs-72695	5	6	establish	establish	VERB
afs-72695	5	7	a	a	DET
afs-72695	5	8	rapid	rapid	ADJ
afs-72695	5	9	method	method	NOUN
afs-72695	5	10	for	for	ADP
afs-72695	5	11	barley	barley	NOUN
afs-72695	5	12	(	(	PUNCT
afs-72695	5	13	hordeum	hordeum	PROPN
afs-72695	5	14	vulgare	vulgare	PROPN
afs-72695	5	15	l.	l.	PROPN
afs-72695	5	16	)	)	PUNCT
afs-72695	5	17	protoplast	protoplast	NOUN
afs-72695	5	18	isolation	isolation	NOUN
afs-72695	5	19	to	to	PART
afs-72695	5	20	provide	provide	VERB
afs-72695	5	21	an	an	DET
afs-72695	5	22	easy	easy	ADJ
afs-72695	5	23	-	-	PUNCT
afs-72695	5	24	to	to	ADP
afs-72695	5	25	-	-	PUNCT
afs-72695	5	26	use	use	NOUN
afs-72695	5	27	procedure	procedure	NOUN
afs-72695	5	28	for	for	ADP
afs-72695	5	29	the	the	DET
afs-72695	5	30	transformation	transformation	NOUN
afs-72695	5	31	and	and	CCONJ
afs-72695	5	32	primary	primary	ADJ
afs-72695	5	33	investigation	investigation	NOUN
afs-72695	5	34	of	of	ADP
afs-72695	5	35	new	new	ADJ
afs-72695	5	36	gene	gene	NOUN
afs-72695	5	37	constructs	construct	NOUN
afs-72695	5	38	by	by	ADP
afs-72695	5	39	transient	transient	ADJ
afs-72695	5	40	gene	gene	NOUN
afs-72695	5	41	expression	expression	NOUN
afs-72695	5	42	assays	assay	NOUN
afs-72695	5	43	.	.	PUNCT
afs-72695	6	1	protoplasts	protoplast	NOUN
afs-72695	6	2	were	be	AUX
afs-72695	6	3	successfully	successfully	ADV
afs-72695	6	4	isolated	isolate	VERB
afs-72695	6	5	from	from	ADP
afs-72695	6	6	the	the	DET
afs-72695	6	7	chopped	chop	VERB
afs-72695	6	8	embryo	embryo	NOUN
afs-72695	6	9	and	and	CCONJ
afs-72695	6	10	scutellum	scutellum	NOUN
afs-72695	6	11	parts	part	NOUN
afs-72695	6	12	of	of	ADP
afs-72695	6	13	mature	mature	ADJ
afs-72695	6	14	barley	barley	NOUN
afs-72695	6	15	seeds	seed	NOUN
afs-72695	6	16	by	by	ADP
afs-72695	6	17	digesting	digest	VERB
afs-72695	6	18	three	three	NUM
afs-72695	6	19	hours	hour	NOUN
afs-72695	6	20	with	with	ADP
afs-72695	6	21	an	an	DET
afs-72695	6	22	enzyme	enzyme	NOUN
afs-72695	6	23	mixture	mixture	NOUN
afs-72695	6	24	.	.	PUNCT
afs-72695	7	1	isolated	isolate	VERB
afs-72695	7	2	protoplasts	protoplast	NOUN
afs-72695	7	3	were	be	AUX
afs-72695	7	4	washed	wash	VERB
afs-72695	7	5	in	in	ADP
afs-72695	7	6	w	w	NOUN
afs-72695	7	7	5	5	NUM
afs-72695	7	8	washing	washing	NOUN
afs-72695	7	9	solution	solution	NOUN
afs-72695	7	10	,	,	PUNCT
afs-72695	7	11	sieved	sieve	VERB
afs-72695	7	12	through	through	ADP
afs-72695	7	13	plastic	plastic	ADJ
afs-72695	7	14	meshes	mesh	NOUN
afs-72695	7	15	and	and	CCONJ
afs-72695	7	16	then	then	ADV
afs-72695	7	17	cleaned	clean	VERB
afs-72695	7	18	on	on	ADP
afs-72695	7	19	sucrose	sucrose	PROPN
afs-72695	7	20	gradient	gradient	NOUN
afs-72695	7	21	.	.	PUNCT
afs-72695	8	1	the	the	DET
afs-72695	8	2	suitability	suitability	NOUN
afs-72695	8	3	of	of	ADP
afs-72695	8	4	these	these	PRON
afs-72695	8	5	directly	directly	ADV
afs-72695	8	6	from	from	ADP
afs-72695	8	7	embryo	embryo	NOUN
afs-72695	8	8	-	-	PUNCT
afs-72695	8	9	scutellum	scutellum	NOUN
afs-72695	8	10	complexes	complex	NOUN
afs-72695	8	11	derived	derive	VERB
afs-72695	8	12	protoplasts	protoplast	NOUN
afs-72695	8	13	for	for	ADP
afs-72695	8	14	transient	transient	ADJ
afs-72695	8	15	gene	gene	NOUN
afs-72695	8	16	expression	expression	NOUN
afs-72695	8	17	studies	study	NOUN
afs-72695	8	18	was	be	AUX
afs-72695	8	19	determined	determine	VERB
afs-72695	8	20	by	by	ADP
afs-72695	8	21	transforming	transform	VERB
afs-72695	8	22	the	the	DET
afs-72695	8	23	protoplasts	protoplast	NOUN
afs-72695	8	24	using	use	VERB
afs-72695	8	25	the	the	DET
afs-72695	8	26	peg	peg	NOUN
afs-72695	8	27	(	(	PUNCT
afs-72695	8	28	polyethylene	polyethylene	NOUN
afs-72695	8	29	glycol	glycol	NOUN
afs-72695	8	30	)	)	PUNCT
afs-72695	8	31	method	method	NOUN
afs-72695	8	32	.	.	PUNCT
afs-72695	9	1	plasmid	plasmid	ADJ
afs-72695	9	2	pactl	pactl	NOUN
afs-72695	9	3	-	-	PUNCT
afs-72695	9	4	f	f	PROPN
afs-72695	9	5	containing	contain	VERB
afs-72695	9	6	the	the	DET
afs-72695	9	7	rice	rice	NOUN
afs-72695	9	8	actl	actl	NOUN
afs-72695	9	9	promoter	promoter	NOUN
afs-72695	9	10	linked	link	VERB
afs-72695	9	11	with	with	ADP
afs-72695	9	12	the	the	DET
afs-72695	9	13	gus	gus	NOUN
afs-72695	9	14	coding	code	VERB
afs-72695	9	15	sequences	sequence	NOUN
afs-72695	9	16	and	and	CCONJ
afs-72695	9	17	the	the	DET
afs-72695	9	18	nos	nos	X
afs-72695	9	19	polyadenylation	polyadenylation	NOUN
afs-72695	9	20	signal	signal	NOUN
afs-72695	9	21	was	be	AUX
afs-72695	9	22	used	use	VERB
afs-72695	9	23	in	in	ADP
afs-72695	9	24	the	the	DET
afs-72695	9	25	transformation	transformation	NOUN
afs-72695	9	26	.	.	PUNCT
afs-72695	10	1	after	after	SCONJ
afs-72695	10	2	the	the	DET
afs-72695	10	3	peg	peg	NOUN
afs-72695	10	4	treatment	treatment	NOUN
afs-72695	10	5	protoplasts	protoplast	NOUN
afs-72695	10	6	were	be	AUX
afs-72695	10	7	cultured	cultured	ADJ
afs-72695	10	8	on	on	ADP
afs-72695	10	9	kpr	kpr	PROPN
afs-72695	10	10	culture	culture	PROPN
afs-72695	10	11	medium	medium	NOUN
afs-72695	10	12	and	and	CCONJ
afs-72695	10	13	the	the	DET
afs-72695	10	14	transient	transient	ADJ
afs-72695	10	15	gus	gus	NOUN
afs-72695	10	16	expression	expression	NOUN
afs-72695	10	17	was	be	AUX
afs-72695	10	18	assayed	assayed	ADJ
afs-72695	10	19	24	24	NUM
afs-72695	10	20	-	-	SYM
afs-72695	10	21	36	36	NUM
afs-72695	10	22	hours	hour	NOUN
afs-72695	10	23	after	after	ADP
afs-72695	10	24	transformation	transformation	NOUN
afs-72695	10	25	.	.	PUNCT
afs-72695	11	1	up	up	ADP
afs-72695	11	2	to	to	PART
afs-72695	11	3	6	6	NUM
afs-72695	11	4	%	%	NOUN
afs-72695	11	5	of	of	ADP
afs-72695	11	6	the	the	DET
afs-72695	11	7	transformed	transform	VERB
afs-72695	11	8	protoplasts	protoplast	NOUN
afs-72695	11	9	showed	show	VERB
afs-72695	11	10	gus	gus	NOUN
afs-72695	11	11	expression	expression	NOUN
afs-72695	11	12	after	after	ADP
afs-72695	11	13	treating	treat	VERB
afs-72695	11	14	the	the	DET
afs-72695	11	15	protoplasts	protoplast	NOUN
afs-72695	11	16	with	with	ADP
afs-72695	11	17	x	x	NOUN
afs-72695	11	18	-	-	NOUN
afs-72695	11	19	gluc	gluc	NOUN
afs-72695	11	20	.	.	PUNCT
afs-72695	12	1	the	the	DET
afs-72695	12	2	results	result	NOUN
afs-72695	12	3	of	of	ADP
afs-72695	12	4	this	this	DET
afs-72695	12	5	study	study	NOUN
afs-72695	12	6	show	show	VERB
afs-72695	12	7	that	that	SCONJ
afs-72695	12	8	the	the	DET
afs-72695	12	9	protoplasts	protoplast	NOUN
afs-72695	12	10	isolated	isolate	VERB
afs-72695	12	11	directly	directly	ADV
afs-72695	12	12	from	from	ADP
afs-72695	12	13	dissected	dissect	VERB
afs-72695	12	14	mature	mature	ADJ
afs-72695	12	15	barley	barley	NOUN
afs-72695	12	16	scutellum	scutellum	NOUN
afs-72695	12	17	-	-	PUNCT
afs-72695	12	18	embryo	embryo	NOUN
afs-72695	12	19	complexes	complex	NOUN
afs-72695	12	20	could	could	AUX
afs-72695	12	21	be	be	AUX
afs-72695	12	22	used	use	VERB
afs-72695	12	23	to	to	PART
afs-72695	12	24	investigate	investigate	VERB
afs-72695	12	25	transient	transient	ADJ
afs-72695	12	26	gene	gene	NOUN
afs-72695	12	27	expressions	expression	NOUN
afs-72695	12	28	in	in	ADP
afs-72695	12	29	barley	barley	NOUN
afs-72695	12	30	.	.	PUNCT
afs-72695	13	1	this	this	DET
afs-72695	13	2	procedure	procedure	NOUN
afs-72695	13	3	requires	require	VERB
afs-72695	13	4	negligible	negligible	ADJ
afs-72695	13	5	time	time	NOUN
afs-72695	13	6	prior	prior	ADV
afs-72695	13	7	the	the	DET
afs-72695	13	8	transformation	transformation	NOUN
afs-72695	13	9	experiment	experiment	NOUN
afs-72695	13	10	and	and	CCONJ
afs-72695	13	11	so	so	ADV
afs-72695	13	12	can	can	AUX
afs-72695	13	13	be	be	AUX
afs-72695	13	14	done	do	VERB
afs-72695	13	15	in	in	ADP
afs-72695	13	16	a	a	DET
afs-72695	13	17	very	very	ADV
afs-72695	13	18	short	short	ADJ
afs-72695	13	19	time	time	NOUN
afs-72695	13	20	compared	compare	VERB
afs-72695	13	21	to	to	ADP
afs-72695	13	22	the	the	DET
afs-72695	13	23	protoplast	protoplast	NOUN
afs-72695	13	24	system	system	NOUN
afs-72695	13	25	based	base	VERB
afs-72695	13	26	on	on	ADP
afs-72695	13	27	a	a	DET
afs-72695	13	28	suspension	suspension	NOUN
afs-72695	13	29	culture	culture	NOUN
afs-72695	13	30	.	.	PUNCT
afs-72695	14	1	key	key	ADJ
afs-72695	14	2	words	word	NOUN
afs-72695	14	3	:	:	PUNCT
afs-72695	14	4	hordeum	hordeum	PROPN
afs-72695	14	5	vulgare	vulgare	PROPN
afs-72695	14	6	l.	l.	PROPN
afs-72695	14	7	,	,	PUNCT
afs-72695	14	8	plasmid	plasmid	NOUN
afs-72695	14	9	,	,	PUNCT
afs-72695	14	10	gene	gene	NOUN
afs-72695	14	11	transfer	transfer	NOUN
afs-72695	14	12	,	,	PUNCT
afs-72695	14	13	(	(	PUNCT
afs-72695	14	14	i	i	NOUN
afs-72695	14	15	-	-	PUNCT
afs-72695	14	16	glucuronidase	glucuronidase	PROPN
afs-72695	14	17	introduction	introduction	NOUN
afs-72695	14	18	during	during	ADP
afs-72695	14	19	the	the	DET
afs-72695	14	20	last	last	ADJ
afs-72695	14	21	decade	decade	NOUN
afs-72695	14	22	much	much	ADJ
afs-72695	14	23	interest	interest	NOUN
afs-72695	14	24	has	have	AUX
afs-72695	14	25	been	be	AUX
afs-72695	14	26	focused	focus	VERB
afs-72695	14	27	on	on	ADP
afs-72695	14	28	the	the	DET
afs-72695	14	29	culture	culture	NOUN
afs-72695	14	30	of	of	ADP
afs-72695	14	31	plant	plant	NOUN
afs-72695	14	32	protoplasts	protoplast	NOUN
afs-72695	14	33	and	and	CCONJ
afs-72695	14	34	their	their	PRON
afs-72695	14	35	potential	potential	NOUN
afs-72695	14	36	in	in	ADP
afs-72695	14	37	producing	produce	VERB
afs-72695	14	38	transgenic	transgenic	NOUN
afs-72695	14	39	plants	plant	NOUN
afs-72695	14	40	as	as	ADV
afs-72695	14	41	well	well	ADV
afs-72695	14	42	as	as	ADP
afs-72695	14	43	in	in	ADP
afs-72695	14	44	assaying	assay	VERB
afs-72695	14	45	the	the	DET
afs-72695	14	46	expression	expression	NOUN
afs-72695	14	47	of	of	ADP
afs-72695	14	48	recombinant	recombinant	ADJ
afs-72695	14	49	gene	gene	NOUN
afs-72695	14	50	constructs	construct	NOUN
afs-72695	14	51	in	in	ADP
afs-72695	14	52	transformed	transform	VERB
afs-72695	14	53	cells	cell	NOUN
afs-72695	14	54	.	.	PUNCT
afs-72695	15	1	the	the	DET
afs-72695	15	2	use	use	NOUN
afs-72695	15	3	of	of	ADP
afs-72695	15	4	protoplasts	protoplast	NOUN
afs-72695	15	5	provides	provide	VERB
afs-72695	15	6	the	the	DET
afs-72695	15	7	advantages	advantage	NOUN
afs-72695	15	8	of	of	ADP
afs-72695	15	9	easier	easy	ADJ
afs-72695	15	10	uptake	uptake	NOUN
afs-72695	15	11	of	of	ADP
afs-72695	15	12	foreign	foreign	ADJ
afs-72695	15	13	dna	dna	NOUN
afs-72695	15	14	by	by	ADP
afs-72695	15	15	the	the	DET
afs-72695	15	16	target	target	NOUN
afs-72695	15	17	cell	cell	NOUN
afs-72695	15	18	in	in	ADP
afs-72695	15	19	the	the	DET
afs-72695	15	20	absence	absence	NOUN
afs-72695	15	21	of	of	ADP
afs-72695	15	22	the	the	DET
afs-72695	15	23	cell	cell	NOUN
afs-72695	15	24	wall	wall	NOUN
afs-72695	15	25	and	and	CCONJ
afs-72695	15	26	also	also	ADV
afs-72695	15	27	single	single	ADJ
afs-72695	15	28	cell	cell	NOUN
afs-72695	15	29	origin	origin	NOUN
afs-72695	15	30	of	of	ADP
afs-72695	15	31	regenerants	regenerant	NOUN
afs-72695	15	32	thus	thus	ADV
afs-72695	15	33	avoiding	avoid	VERB
afs-72695	15	34	chimerism	chimerism	NOUN
afs-72695	15	35	in	in	ADP
afs-72695	15	36	transformants	transformant	NOUN
afs-72695	15	37	.	.	PUNCT
afs-72695	16	1	considerable	considerable	ADJ
afs-72695	16	2	progress	progress	NOUN
afs-72695	16	3	has	have	AUX
afs-72695	16	4	been	be	AUX
afs-72695	16	5	made	make	VERB
afs-72695	16	6	in	in	ADP
afs-72695	16	7	protoplast	protoplast	NOUN
afs-72695	16	8	culture	culture	NOUN
afs-72695	16	9	of	of	ADP
afs-72695	16	10	important	important	ADJ
afs-72695	16	11	monocot	monocot	ADJ
afs-72695	16	12	species	specie	NOUN
afs-72695	16	13	and	and	CCONJ
afs-72695	16	14	the	the	DET
afs-72695	16	15	first	first	ADJ
afs-72695	16	16	protoplast	protoplast	NOUN
afs-72695	16	17	derived	derive	VERB
afs-72695	16	18	regeneration	regeneration	NOUN
afs-72695	16	19	in	in	ADP
afs-72695	16	20	cereals	cereal	NOUN
afs-72695	16	21	was	be	AUX
afs-72695	16	22	reported	report	VERB
afs-72695	16	23	in	in	ADP
afs-72695	16	24	rice	rice	NOUN
afs-72695	16	25	(	(	PUNCT
afs-72695	16	26	oryza	oryza	PROPN
afs-72695	16	27	saliva	saliva	PROPN
afs-72695	16	28	l.	l.	PROPN
afs-72695	16	29	)	)	PUNCT
afs-72695	16	30	by	by	ADP
afs-72695	16	31	fujimura	fujimura	NOUN
afs-72695	16	32	and	and	CCONJ
afs-72695	16	33	his	his	PRON
afs-72695	16	34	group	group	NOUN
afs-72695	16	35	(	(	PUNCT
afs-72695	16	36	1985	1985	NUM
afs-72695	16	37	)	)	PUNCT
afs-72695	16	38	.	.	PUNCT
afs-72695	17	1	succesful	succesful	ADJ
afs-72695	17	2	protoplast	protoplast	NOUN
afs-72695	17	3	-	-	PUNCT
afs-72695	17	4	plant	plant	NOUN
afs-72695	17	5	regeneration	regeneration	NOUN
afs-72695	17	6	systems	system	NOUN
afs-72695	17	7	have	have	AUX
afs-72695	17	8	also	also	ADV
afs-72695	17	9	been	be	AUX
afs-72695	17	10	reported	report	VERB
afs-72695	17	11	in	in	ADP
afs-72695	17	12	wheat	wheat	NOUN
afs-72695	17	13	(	(	PUNCT
afs-72695	17	14	vasil	vasil	NOUN
afs-72695	17	15	et	et	PROPN
afs-72695	17	16	al	al	PROPN
afs-72695	17	17	.	.	PROPN
afs-72695	17	18	1990	1990	NUM
afs-72695	17	19	,	,	PUNCT
afs-72695	17	20	chang	chang	PROPN
afs-72695	17	21	et	et	PROPN
afs-72695	17	22	al	al	PROPN
afs-72695	17	23	.	.	PROPN
afs-72695	17	24	1991	1991	NUM
afs-72695	17	25	,	,	PUNCT
afs-72695	17	26	yang	yang	PROPN
afs-72695	17	27	et	et	PROPN
afs-72695	17	28	al	al	PROPN
afs-72695	17	29	.	.	PROPN
afs-72695	17	30	1993	1993	NUM
afs-72695	17	31	,	,	PUNCT
afs-72695	17	32	ahmed	ahmed	PROPN
afs-72695	17	33	and	and	CCONJ
afs-72695	17	34	sagi	sagi	PROPN
afs-72695	17	35	1993	1993	NUM
afs-72695	17	36	,	,	PUNCT
afs-72695	17	37	pauk	pauk	NOUN
afs-72695	17	38	et	et	PROPN
afs-72695	17	39	al	al	PROPN
afs-72695	17	40	.	.	PROPN
afs-72695	17	41	1994	1994	NUM
afs-72695	17	42	)	)	PUNCT
afs-72695	17	43	,	,	PUNCT
afs-72695	17	44	maize	maize	NOUN
afs-72695	17	45	(	(	PUNCT
afs-72695	17	46	mörocz	mörocz	NOUN
afs-72695	17	47	et	et	PROPN
afs-72695	17	48	al	al	PROPN
afs-72695	17	49	.	.	PROPN
afs-72695	17	50	199	199	NUM
afs-72695	17	51	agricultural	agricultural	ADJ
afs-72695	17	52	science	science	NOUN
afs-72695	17	53	in	in	ADP
afs-72695	17	54	finland	finland	PROPN
afs-72695	17	55	3	3	NUM
afs-72695	17	56	(	(	PUNCT
afs-72695	17	57	1994)research	1994)research	NUM
afs-72695	17	58	note	note	NOUN
afs-72695	17	59	https://www.c-info.fi/en/info/?token=xlfur93efmjw2e_x.bmkm1pjy-oyfc5761tkv9g.8q_fdwjvcvxtwujwdbhxmffxfuxny_o1rrs0qrr6mmjkzncbypq8pjglhmxrtb_rfb_opjts7zolsaceycvbrspndjjyvu6g_va8lrcetfdmqfe7wlosfuyfjguqd5_djpp5z8perxmadz2secp9d6jt5wp7zcs-gof6ok9vmg12djxpedbevaqyuxz6eihnl6nvdtu7crviludku6ffdhbosywqcx9aa9-rcd_pjhjvloh3feiatdlpf8l-su9rgu9z6amk5yh8rs4hpp04mtrxcbajww52fpjonzf2xhdc61xwfoia7sfrhhp5k_fjmjkb09v0-cuixe7asjpu	https://www.c-info.fi/en/info/?token=xlfur93efmjw2e_x.bmkm1pjy-oyfc5761tkv9g.8q_fdwjvcvxtwujwdbhxmffxfuxny_o1rrs0qrr6mmjkzncbypq8pjglhmxrtb_rfb_opjts7zolsaceycvbrspndjjyvu6g_va8lrcetfdmqfe7wlosfuyfjguqd5_djpp5z8perxmadz2secp9d6jt5wp7zcs-gof6ok9vmg12djxpedbevaqyuxz6eihnl6nvdtu7crviludku6ffdhbosywqcx9aa9-rcd_pjhjvloh3feiatdlpf8l-su9rgu9z6amk5yh8rs4hpp04mtrxcbajww52fpjonzf2xhdc61xwfoia7sfrhhp5k_fjmjkb09v0-cuixe7asjpu	PROPN
afs-72695	17	60	research	research	NOUN
afs-72695	17	61	note	note	NOUN
afs-72695	17	62	1990	1990	NUM
afs-72695	17	63	)	)	PUNCT
afs-72695	17	64	and	and	CCONJ
afs-72695	17	65	barley	barley	NOUN
afs-72695	17	66	(	(	PUNCT
afs-72695	17	67	jähne	jähne	PROPN
afs-72695	17	68	et	et	PROPN
afs-72695	17	69	al	al	PROPN
afs-72695	17	70	.	.	PROPN
afs-72695	17	71	1991	1991	NUM
afs-72695	17	72	)	)	PUNCT
afs-72695	17	73	.	.	PUNCT
afs-72695	18	1	improvements	improvement	NOUN
afs-72695	18	2	in	in	ADP
afs-72695	18	3	protoplast	protoplast	NOUN
afs-72695	18	4	culture	culture	NOUN
afs-72695	18	5	and	and	CCONJ
afs-72695	18	6	regeneration	regeneration	NOUN
afs-72695	18	7	have	have	AUX
afs-72695	18	8	significantly	significantly	ADV
afs-72695	18	9	given	give	VERB
afs-72695	18	10	a	a	DET
afs-72695	18	11	contribution	contribution	NOUN
afs-72695	18	12	to	to	ADP
afs-72695	18	13	the	the	DET
afs-72695	18	14	genetic	genetic	ADJ
afs-72695	18	15	transformation	transformation	NOUN
afs-72695	18	16	of	of	ADP
afs-72695	18	17	monocots	monocot	NOUN
afs-72695	18	18	.	.	PUNCT
afs-72695	19	1	in	in	ADP
afs-72695	19	2	rice	rice	NOUN
afs-72695	19	3	,	,	PUNCT
afs-72695	19	4	the	the	DET
afs-72695	19	5	transformation	transformation	NOUN
afs-72695	19	6	of	of	ADP
afs-72695	19	7	protoplasts	protoplast	NOUN
afs-72695	19	8	and	and	CCONJ
afs-72695	19	9	the	the	DET
afs-72695	19	10	subsequent	subsequent	ADJ
afs-72695	19	11	regeneration	regeneration	NOUN
afs-72695	19	12	of	of	ADP
afs-72695	19	13	transgenic	transgenic	NOUN
afs-72695	19	14	rice	rice	NOUN
afs-72695	19	15	plants	plant	NOUN
afs-72695	19	16	have	have	AUX
afs-72695	19	17	become	become	VERB
afs-72695	19	18	a	a	DET
afs-72695	19	19	routine	routine	ADJ
afs-72695	19	20	procedure	procedure	NOUN
afs-72695	19	21	(	(	PUNCT
afs-72695	19	22	jenes	jene	VERB
afs-72695	19	23	et	et	PROPN
afs-72695	19	24	al	al	PROPN
afs-72695	19	25	.	.	PROPN
afs-72695	19	26	1992	1992	NUM
afs-72695	19	27	)	)	PUNCT
afs-72695	19	28	.	.	PUNCT
afs-72695	20	1	protoplast	protoplast	NOUN
afs-72695	20	2	transformation	transformation	NOUN
afs-72695	20	3	resulting	result	VERB
afs-72695	20	4	in	in	ADP
afs-72695	20	5	stable	stable	ADJ
afs-72695	20	6	integration	integration	NOUN
afs-72695	20	7	of	of	ADP
afs-72695	20	8	foreign	foreign	ADJ
afs-72695	20	9	genes	gene	NOUN
afs-72695	20	10	into	into	ADP
afs-72695	20	11	the	the	DET
afs-72695	20	12	target	target	NOUN
afs-72695	20	13	cells	cell	NOUN
afs-72695	20	14	has	have	AUX
afs-72695	20	15	also	also	ADV
afs-72695	20	16	been	be	AUX
afs-72695	20	17	reported	report	VERB
afs-72695	20	18	in	in	ADP
afs-72695	20	19	other	other	ADJ
afs-72695	20	20	cereals	cereal	NOUN
afs-72695	20	21	such	such	ADJ
afs-72695	20	22	as	as	ADP
afs-72695	20	23	rice	rice	NOUN
afs-72695	20	24	(	(	PUNCT
afs-72695	20	25	shimamoto	shimamoto	PROPN
afs-72695	20	26	et	et	PROPN
afs-72695	20	27	al	al	PROPN
afs-72695	20	28	.	.	PROPN
afs-72695	20	29	1989	1989	NUM
afs-72695	20	30	)	)	PUNCT
afs-72695	20	31	,	,	PUNCT
afs-72695	20	32	maize	maize	NOUN
afs-72695	20	33	(	(	PUNCT
afs-72695	20	34	mörocz	mörocz	NOUN
afs-72695	20	35	et	et	PROPN
afs-72695	20	36	al	al	PROPN
afs-72695	20	37	.	.	PROPN
afs-72695	20	38	1990	1990	NUM
afs-72695	20	39	)	)	PUNCT
afs-72695	20	40	and	and	CCONJ
afs-72695	20	41	barley	barley	NOUN
afs-72695	20	42	(	(	PUNCT
afs-72695	20	43	lazzeri	lazzeri	NOUN
afs-72695	20	44	and	and	CCONJ
afs-72695	20	45	lörz	lörz	NOUN
afs-72695	20	46	1990	1990	NUM
afs-72695	20	47	,	,	PUNCT
afs-72695	20	48	lazzeri	lazzeri	NOUN
afs-72695	20	49	et	et	PROPN
afs-72695	20	50	al	al	PROPN
afs-72695	20	51	.	.	PROPN
afs-72695	20	52	1992	1992	NUM
afs-72695	20	53	)	)	PUNCT
afs-72695	20	54	.	.	PUNCT
afs-72695	21	1	in	in	ADP
afs-72695	21	2	barley	barley	NOUN
afs-72695	21	3	,	,	PUNCT
afs-72695	21	4	most	most	ADJ
afs-72695	21	5	of	of	ADP
afs-72695	21	6	the	the	DET
afs-72695	21	7	reported	report	VERB
afs-72695	21	8	studies	study	NOUN
afs-72695	21	9	in	in	ADP
afs-72695	21	10	protoplast	protoplast	NOUN
afs-72695	21	11	isolation	isolation	NOUN
afs-72695	21	12	and	and	CCONJ
afs-72695	21	13	transformation	transformation	NOUN
afs-72695	21	14	have	have	AUX
afs-72695	21	15	so	so	ADV
afs-72695	21	16	far	far	ADV
afs-72695	21	17	been	be	AUX
afs-72695	21	18	based	base	VERB
afs-72695	21	19	on	on	ADP
afs-72695	21	20	suspension	suspension	NOUN
afs-72695	21	21	derived	derive	VERB
afs-72695	21	22	protoplasts	protoplast	NOUN
afs-72695	21	23	(	(	PUNCT
afs-72695	21	24	luhrs	luhrs	PROPN
afs-72695	21	25	and	and	CCONJ
afs-72695	21	26	lörz	lörz	NOUN
afs-72695	21	27	1988	1988	NUM
afs-72695	21	28	,	,	PUNCT
afs-72695	21	29	lazzeri	lazzeri	NOUN
afs-72695	21	30	and	and	CCONJ
afs-72695	21	31	lörz	lörz	NOUN
afs-72695	21	32	1990	1990	NUM
afs-72695	21	33	,	,	PUNCT
afs-72695	21	34	jähne	jähne	PROPN
afs-72695	21	35	et	et	PROPN
afs-72695	21	36	al	al	PROPN
afs-72695	21	37	.	.	PROPN
afs-72695	21	38	1991	1991	NUM
afs-72695	21	39	,	,	PUNCT
afs-72695	21	40	lazzeri	lazzeri	NOUN
afs-72695	21	41	et	et	PROPN
afs-72695	21	42	al	al	PROPN
afs-72695	21	43	.	.	PROPN
afs-72695	21	44	1992	1992	NUM
afs-72695	21	45	)	)	PUNCT
afs-72695	21	46	.	.	PUNCT
afs-72695	22	1	junker	junker	PROPN
afs-72695	22	2	et	et	PROPN
afs-72695	22	3	al	al	PROPN
afs-72695	22	4	.	.	PROPN
afs-72695	22	5	(	(	PUNCT
afs-72695	22	6	1987	1987	NUM
afs-72695	22	7	)	)	PUNCT
afs-72695	22	8	detected	detect	VERB
afs-72695	22	9	transient	transient	ADJ
afs-72695	22	10	expression	expression	NOUN
afs-72695	22	11	of	of	ADP
afs-72695	22	12	npt	npt	PROPN
afs-72695	22	13	ii	ii	PROPN
afs-72695	22	14	(	(	PUNCT
afs-72695	22	15	neomycin	neomycin	PROPN
afs-72695	22	16	phosphotransferase	phosphotransferase	PROPN
afs-72695	22	17	ii	ii	PROPN
afs-72695	22	18	)	)	PUNCT
afs-72695	22	19	gene	gene	NOUN
afs-72695	22	20	in	in	ADP
afs-72695	22	21	protoplasts	protoplast	NOUN
afs-72695	22	22	derivedfrom	derivedfrom	VERB
afs-72695	22	23	suspension	suspension	NOUN
afs-72695	22	24	culture	culture	NOUN
afs-72695	22	25	and	and	CCONJ
afs-72695	22	26	transformed	transform	VERB
afs-72695	22	27	with	with	ADP
afs-72695	22	28	peg	peg	NOUN
afs-72695	22	29	.	.	PUNCT
afs-72695	23	1	the	the	DET
afs-72695	23	2	initiation	initiation	NOUN
afs-72695	23	3	and	and	CCONJ
afs-72695	23	4	maintenance	maintenance	NOUN
afs-72695	23	5	of	of	ADP
afs-72695	23	6	fine	fine	ADJ
afs-72695	23	7	suspension	suspension	NOUN
afs-72695	23	8	cultures	culture	NOUN
afs-72695	23	9	,	,	PUNCT
afs-72695	23	10	a	a	DET
afs-72695	23	11	prerequisite	prerequisite	NOUN
afs-72695	23	12	for	for	ADP
afs-72695	23	13	successful	successful	ADJ
afs-72695	23	14	protoplast	protoplast	NOUN
afs-72695	23	15	isolation	isolation	NOUN
afs-72695	23	16	in	in	ADP
afs-72695	23	17	barley	barley	NOUN
afs-72695	23	18	as	as	ADV
afs-72695	23	19	well	well	ADV
afs-72695	23	20	as	as	ADP
afs-72695	23	21	in	in	ADP
afs-72695	23	22	other	other	ADJ
afs-72695	23	23	species	specie	NOUN
afs-72695	23	24	,	,	PUNCT
afs-72695	23	25	is	be	AUX
afs-72695	23	26	laboriuos	laboriuo	NOUN
afs-72695	23	27	and	and	CCONJ
afs-72695	23	28	may	may	AUX
afs-72695	23	29	take	take	VERB
afs-72695	23	30	a	a	DET
afs-72695	23	31	considerably	considerably	ADV
afs-72695	23	32	long	long	ADJ
afs-72695	23	33	time	time	NOUN
afs-72695	23	34	from	from	ADP
afs-72695	23	35	some	some	DET
afs-72695	23	36	weeks	week	NOUN
afs-72695	23	37	to	to	ADP
afs-72695	23	38	several	several	ADJ
afs-72695	23	39	months	month	NOUN
afs-72695	23	40	.	.	PUNCT
afs-72695	24	1	it	it	PRON
afs-72695	24	2	can	can	AUX
afs-72695	24	3	therefore	therefore	ADV
afs-72695	24	4	be	be	AUX
afs-72695	24	5	a	a	DET
afs-72695	24	6	limiting	limit	VERB
afs-72695	24	7	factor	factor	NOUN
afs-72695	24	8	in	in	ADP
afs-72695	24	9	applying	apply	VERB
afs-72695	24	10	barley	barley	NOUN
afs-72695	24	11	protoplasts	protoplast	NOUN
afs-72695	24	12	to	to	PART
afs-72695	24	13	transient	transient	VERB
afs-72695	24	14	gene	gene	NOUN
afs-72695	24	15	expression	expression	NOUN
afs-72695	24	16	studies	study	NOUN
afs-72695	24	17	.	.	PUNCT
afs-72695	25	1	in	in	ADP
afs-72695	25	2	addition	addition	NOUN
afs-72695	25	3	,	,	PUNCT
afs-72695	25	4	a	a	DET
afs-72695	25	5	long	long	ADV
afs-72695	25	6	lasting	last	VERB
afs-72695	25	7	preculture	preculture	NOUN
afs-72695	25	8	phase	phase	NOUN
afs-72695	25	9	before	before	SCONJ
afs-72695	25	10	the	the	DET
afs-72695	25	11	transformation	transformation	NOUN
afs-72695	25	12	may	may	AUX
afs-72695	25	13	result	result	VERB
afs-72695	25	14	in	in	ADP
afs-72695	25	15	undesirable	undesirable	ADJ
afs-72695	25	16	somaclonal	somaclonal	ADJ
afs-72695	25	17	variation	variation	NOUN
afs-72695	25	18	at	at	ADP
afs-72695	25	19	the	the	DET
afs-72695	25	20	level	level	NOUN
afs-72695	25	21	of	of	ADP
afs-72695	25	22	transgenic	transgenic	NOUN
afs-72695	25	23	regenerants	regenerant	NOUN
afs-72695	25	24	.	.	PUNCT
afs-72695	26	1	therefore	therefore	ADV
afs-72695	26	2	the	the	DET
afs-72695	26	3	use	use	NOUN
afs-72695	26	4	of	of	ADP
afs-72695	26	5	protoplasts	protoplast	NOUN
afs-72695	26	6	in	in	ADP
afs-72695	26	7	transformation	transformation	NOUN
afs-72695	26	8	experiments	experiment	NOUN
afs-72695	26	9	would	would	AUX
afs-72695	26	10	benifit	benifit	VERB
afs-72695	26	11	from	from	ADP
afs-72695	26	12	a	a	DET
afs-72695	26	13	procedure	procedure	NOUN
afs-72695	26	14	that	that	PRON
afs-72695	26	15	would	would	AUX
afs-72695	26	16	reduce	reduce	VERB
afs-72695	26	17	the	the	DET
afs-72695	26	18	time	time	NOUN
afs-72695	26	19	in	in	ADP
afs-72695	26	20	culture	culture	NOUN
afs-72695	26	21	prior	prior	ADV
afs-72695	26	22	to	to	ADP
afs-72695	26	23	the	the	DET
afs-72695	26	24	gene	gene	NOUN
afs-72695	26	25	transfer	transfer	NOUN
afs-72695	26	26	.	.	PUNCT
afs-72695	27	1	diaz	diaz	PROPN
afs-72695	27	2	and	and	CCONJ
afs-72695	27	3	carbonero	carbonero	PROPN
afs-72695	27	4	(	(	PUNCT
afs-72695	27	5	1992	1992	NUM
afs-72695	27	6	)	)	PUNCT
afs-72695	27	7	investigated	investigate	VERB
afs-72695	27	8	tissue	tissue	NOUN
afs-72695	27	9	specific	specific	ADJ
afs-72695	27	10	transient	transient	ADJ
afs-72695	27	11	expression	expression	NOUN
afs-72695	27	12	of	of	ADP
afs-72695	27	13	the	the	DET
afs-72695	27	14	gus	gus	NOUN
afs-72695	27	15	reporter	reporter	NOUN
afs-72695	27	16	gene	gene	NOUN
afs-72695	27	17	in	in	ADP
afs-72695	27	18	transformed	transform	VERB
afs-72695	27	19	barley	barley	NOUN
afs-72695	27	20	protoplasts	protoplast	NOUN
afs-72695	27	21	isolated	isolate	VERB
afs-72695	27	22	from	from	ADP
afs-72695	27	23	developing	develop	VERB
afs-72695	27	24	endosperm	endosperm	NOUN
afs-72695	27	25	.	.	PUNCT
afs-72695	28	1	there	there	PRON
afs-72695	28	2	are	be	VERB
afs-72695	28	3	reports	report	NOUN
afs-72695	28	4	on	on	ADP
afs-72695	28	5	the	the	DET
afs-72695	28	6	isolation	isolation	NOUN
afs-72695	28	7	of	of	ADP
afs-72695	28	8	barley	barley	NOUN
afs-72695	28	9	aleurone	aleurone	NOUN
afs-72695	28	10	protoplasts	protoplast	NOUN
afs-72695	28	11	for	for	ADP
afs-72695	28	12	transient	transient	ADJ
afs-72695	28	13	expression	expression	NOUN
afs-72695	28	14	studies	study	NOUN
afs-72695	28	15	(	(	PUNCT
afs-72695	28	16	skriver	skriver	X
afs-72695	28	17	et	et	PROPN
afs-72695	28	18	al	al	PROPN
afs-72695	28	19	.	.	PROPN
afs-72695	28	20	1991	1991	NUM
afs-72695	28	21	)	)	PUNCT
afs-72695	28	22	.	.	PUNCT
afs-72695	29	1	this	this	DET
afs-72695	29	2	isolation	isolation	NOUN
afs-72695	29	3	procedure	procedure	NOUN
afs-72695	29	4	,	,	PUNCT
afs-72695	29	5	however	however	ADV
afs-72695	29	6	,	,	PUNCT
afs-72695	29	7	requires	require	VERB
afs-72695	29	8	very	very	ADV
afs-72695	29	9	specific	specific	ADJ
afs-72695	29	10	skills	skill	NOUN
afs-72695	29	11	.	.	PUNCT
afs-72695	30	1	in	in	ADP
afs-72695	30	2	this	this	DET
afs-72695	30	3	paper	paper	NOUN
afs-72695	30	4	we	we	PRON
afs-72695	30	5	introduce	introduce	VERB
afs-72695	30	6	a	a	DET
afs-72695	30	7	quick	quick	ADJ
afs-72695	30	8	method	method	NOUN
afs-72695	30	9	to	to	PART
afs-72695	30	10	isolate	isolate	VERB
afs-72695	30	11	barley	barley	NOUN
afs-72695	30	12	protoplasts	protoplast	NOUN
afs-72695	30	13	directly	directly	ADV
afs-72695	30	14	from	from	ADP
afs-72695	30	15	dissected	dissect	VERB
afs-72695	30	16	mature	mature	ADJ
afs-72695	30	17	scutellumembryo	scutellumembryo	NOUN
afs-72695	30	18	complexes	complex	NOUN
afs-72695	30	19	and	and	CCONJ
afs-72695	30	20	the	the	DET
afs-72695	30	21	use	use	NOUN
afs-72695	30	22	of	of	ADP
afs-72695	30	23	such	such	ADJ
afs-72695	30	24	protoplasts	protoplast	NOUN
afs-72695	30	25	in	in	ADP
afs-72695	30	26	transient	transient	ADJ
afs-72695	30	27	gene	gene	NOUN
afs-72695	30	28	expression	expression	NOUN
afs-72695	30	29	studies	study	NOUN
afs-72695	30	30	.	.	PUNCT
afs-72695	31	1	this	this	DET
afs-72695	31	2	isolation	isolation	NOUN
afs-72695	31	3	method	method	NOUN
afs-72695	31	4	was	be	AUX
afs-72695	31	5	found	find	VERB
afs-72695	31	6	to	to	PART
afs-72695	31	7	be	be	AUX
afs-72695	31	8	equivalent	equivalent	ADJ
afs-72695	31	9	in	in	ADP
afs-72695	31	10	efficiency	efficiency	NOUN
afs-72695	31	11	to	to	ADP
afs-72695	31	12	the	the	DET
afs-72695	31	13	transformation	transformation	NOUN
afs-72695	31	14	of	of	ADP
afs-72695	31	15	protoplasts	protoplast	NOUN
afs-72695	31	16	derived	derive	VERB
afs-72695	31	17	from	from	ADP
afs-72695	31	18	cell	cell	NOUN
afs-72695	31	19	suspension	suspension	NOUN
afs-72695	31	20	but	but	CCONJ
afs-72695	31	21	the	the	DET
afs-72695	31	22	time	time	NOUN
afs-72695	31	23	requirement	requirement	NOUN
afs-72695	31	24	was	be	AUX
afs-72695	31	25	negligible	negligible	ADJ
afs-72695	31	26	compared	compare	VERB
afs-72695	31	27	to	to	ADP
afs-72695	31	28	the	the	DET
afs-72695	31	29	suspension	suspension	NOUN
afs-72695	31	30	cultures	culture	NOUN
afs-72695	31	31	.	.	PUNCT
afs-72695	32	1	material	material	NOUN
afs-72695	32	2	and	and	CCONJ
afs-72695	32	3	methods	method	NOUN
afs-72695	32	4	plant	plant	NOUN
afs-72695	32	5	material	material	NOUN
afs-72695	32	6	dry	dry	ADJ
afs-72695	32	7	seeds	seed	NOUN
afs-72695	32	8	of	of	ADP
afs-72695	32	9	barley	barley	NOUN
afs-72695	32	10	cultivars	cultivar	NOUN
afs-72695	32	11	‘	'	PUNCT
afs-72695	32	12	pohto	pohto	NOUN
afs-72695	32	13	’	'	PUNCT
afs-72695	32	14	,	,	PUNCT
afs-72695	32	15	‘	'	PUNCT
afs-72695	32	16	kymppi	kymppi	X
afs-72695	32	17	’	'	PUNCT
afs-72695	32	18	,	,	PUNCT
afs-72695	32	19	‘	'	PUNCT
afs-72695	32	20	prisma	prisma	NOUN
afs-72695	32	21	’	'	PUNCT
afs-72695	32	22	and	and	CCONJ
afs-72695	32	23	tgri	tgri	NOUN
afs-72695	32	24	’	'	PUNCT
afs-72695	32	25	were	be	AUX
afs-72695	32	26	provided	provide	VERB
afs-72695	32	27	by	by	ADP
afs-72695	32	28	the	the	DET
afs-72695	32	29	institute	institute	NOUN
afs-72695	32	30	of	of	ADP
afs-72695	32	31	plant	plant	NOUN
afs-72695	32	32	breeding	breeding	NOUN
afs-72695	32	33	,	,	PUNCT
afs-72695	32	34	agricultural	agricultural	ADJ
afs-72695	32	35	research	research	NOUN
afs-72695	32	36	centre	centre	PROPN
afs-72695	32	37	offinland	offinland	PROPN
afs-72695	32	38	,	,	PUNCT
afs-72695	32	39	jokioinen	jokioinen	PROPN
afs-72695	32	40	.	.	PUNCT
afs-72695	33	1	protoplast	protoplast	NOUN
afs-72695	33	2	isolation	isolation	NOUN
afs-72695	33	3	dry	dry	ADJ
afs-72695	33	4	seeds	seed	NOUN
afs-72695	33	5	of	of	ADP
afs-72695	33	6	barley	barley	NOUN
afs-72695	33	7	were	be	AUX
afs-72695	33	8	surface	surface	NOUN
afs-72695	33	9	sterilized	sterilize	VERB
afs-72695	33	10	in	in	ADP
afs-72695	33	11	0.01	0.01	NUM
afs-72695	33	12	%	%	NOUN
afs-72695	33	13	solution	solution	NOUN
afs-72695	33	14	of	of	ADP
afs-72695	33	15	mercuric	mercuric	ADJ
afs-72695	33	16	chloride	chloride	NOUN
afs-72695	33	17	and	and	CCONJ
afs-72695	33	18	then	then	ADV
afs-72695	33	19	washed	wash	VERB
afs-72695	33	20	and	and	CCONJ
afs-72695	33	21	rinsed	rinse	VERB
afs-72695	33	22	with	with	ADP
afs-72695	33	23	sterile	sterile	ADJ
afs-72695	33	24	distilled	distil	VERB
afs-72695	33	25	water	water	NOUN
afs-72695	33	26	for	for	ADP
afs-72695	33	27	seven	seven	NUM
afs-72695	33	28	times	time	NOUN
afs-72695	33	29	.	.	PUNCT
afs-72695	34	1	after	after	ADP
afs-72695	34	2	sterilization	sterilization	NOUN
afs-72695	34	3	the	the	DET
afs-72695	34	4	seeds	seed	NOUN
afs-72695	34	5	were	be	AUX
afs-72695	34	6	imbibed	imbibe	VERB
afs-72695	34	7	in	in	ADP
afs-72695	34	8	sterile	sterile	ADJ
afs-72695	34	9	distilled	distil	VERB
afs-72695	34	10	water	water	NOUN
afs-72695	34	11	and	and	CCONJ
afs-72695	34	12	incubated	incubate	VERB
afs-72695	34	13	in	in	ADP
afs-72695	34	14	25	25	NUM
afs-72695	34	15	°	°	NOUN
afs-72695	34	16	c	c	NOUN
afs-72695	34	17	for	for	ADP
afs-72695	34	18	24	24	NUM
afs-72695	34	19	-	-	SYM
afs-72695	34	20	36	36	NUM
afs-72695	34	21	hours	hour	NOUN
afs-72695	34	22	.	.	PUNCT
afs-72695	35	1	the	the	DET
afs-72695	35	2	imbibed	imbibe	VERB
afs-72695	35	3	embryoscutellum	embryoscutellum	NOUN
afs-72695	35	4	complexes	complex	NOUN
afs-72695	35	5	were	be	AUX
afs-72695	35	6	excised	excise	VERB
afs-72695	35	7	from	from	ADP
afs-72695	35	8	the	the	DET
afs-72695	35	9	seeds	seed	NOUN
afs-72695	35	10	and	and	CCONJ
afs-72695	35	11	chopped	chop	VERB
afs-72695	35	12	into	into	ADP
afs-72695	35	13	small	small	ADJ
afs-72695	35	14	species	specie	NOUN
afs-72695	35	15	with	with	ADP
afs-72695	35	16	a	a	DET
afs-72695	35	17	scalpel	scalpel	NOUN
afs-72695	35	18	(	(	PUNCT
afs-72695	35	19	fig	fig	NOUN
afs-72695	35	20	.	.	PUNCT
afs-72695	36	1	1	1	NUM
afs-72695	36	2	)	)	PUNCT
afs-72695	36	3	.	.	PUNCT
afs-72695	37	1	the	the	DET
afs-72695	37	2	following	follow	VERB
afs-72695	37	3	three	three	NUM
afs-72695	37	4	enzyme	enzyme	NOUN
afs-72695	37	5	mixtures	mixture	NOUN
afs-72695	37	6	were	be	AUX
afs-72695	37	7	originally	originally	ADV
afs-72695	37	8	used	use	VERB
afs-72695	37	9	for	for	ADP
afs-72695	37	10	the	the	DET
afs-72695	37	11	digestion	digestion	NOUN
afs-72695	37	12	of	of	ADP
afs-72695	37	13	protoplasts	protoplast	NOUN
afs-72695	37	14	:	:	PUNCT
afs-72695	37	15	1	1	NUM
afs-72695	37	16	)	)	PUNCT
afs-72695	37	17	je	je	NOUN
afs-72695	37	18	enzyme	enzyme	NOUN
afs-72695	37	19	mixture	mixture	NOUN
afs-72695	37	20	modified	modify	VERB
afs-72695	37	21	after	after	ADP
afs-72695	37	22	junker	junker	PROPN
afs-72695	37	23	et	et	PROPN
afs-72695	37	24	al	al	PROPN
afs-72695	37	25	.	.	PROPN
afs-72695	38	1	(	(	PUNCT
afs-72695	38	2	1987	1987	NUM
afs-72695	38	3	)	)	PUNCT
afs-72695	38	4	containing	contain	VERB
afs-72695	38	5	1.0	1.0	NUM
afs-72695	38	6	%	%	NOUN
afs-72695	38	7	onozuka	onozuka	NOUN
afs-72695	38	8	rs	rs	NOUN
afs-72695	38	9	cellulase	cellulase	NOUN
afs-72695	38	10	,	,	PUNCT
afs-72695	38	11	0.5	0.5	NUM
afs-72695	38	12	%	%	NOUN
afs-72695	38	13	macerozyme	macerozyme	NOUN
afs-72695	38	14	r	r	NOUN
afs-72695	38	15	10	10	NUM
afs-72695	38	16	(	(	PUNCT
afs-72695	38	17	serva	serva	PROPN
afs-72695	38	18	)	)	PUNCT
afs-72695	38	19	,	,	PUNCT
afs-72695	38	20	0.05	0.05	NUM
afs-72695	38	21	%	%	NOUN
afs-72695	38	22	pectolyase	pectolyase	NOUN
afs-72695	38	23	y23	y23	NOUN
afs-72695	38	24	(	(	PUNCT
afs-72695	38	25	seishin	seishin	PROPN
afs-72695	38	26	)	)	PUNCT
afs-72695	38	27	,	,	PUNCT
afs-72695	38	28	5	5	NUM
afs-72695	38	29	mm	mm	PROPN
afs-72695	38	30	cacl	cacl	PROPN
afs-72695	38	31	,	,	PUNCT
afs-72695	38	32	,	,	PUNCT
afs-72695	38	33	0.5	0.5	NUM
afs-72695	38	34	mm	mm	NOUN
afs-72695	38	35	na	na	PROPN
afs-72695	38	36	2	2	NUM
afs-72695	38	37	hp04	hp04	PROPN
afs-72695	38	38	,	,	PUNCT
afs-72695	38	39	at	at	ADP
afs-72695	38	40	ph	ph	PROPN
afs-72695	38	41	5.8	5.8	NUM
afs-72695	38	42	,	,	PUNCT
afs-72695	38	43	2	2	NUM
afs-72695	38	44	)	)	PUNCT
afs-72695	38	45	ke	ke	NOUN
afs-72695	38	46	enzyme	enzyme	NOUN
afs-72695	38	47	mixture	mixture	NOUN
afs-72695	38	48	modified	modify	VERB
afs-72695	38	49	after	after	ADP
afs-72695	38	50	ahmed	ahmed	PROPN
afs-72695	38	51	and	and	CCONJ
afs-72695	38	52	sägi	sägi	PROPN
afs-72695	38	53	(	(	PUNCT
afs-72695	38	54	1993	1993	NUM
afs-72695	38	55	)	)	PUNCT
afs-72695	38	56	containing	contain	VERB
afs-72695	38	57	2	2	NUM
afs-72695	38	58	%	%	NOUN
afs-72695	38	59	onozuka	onozuka	NOUN
afs-72695	38	60	rs	rs	NOUN
afs-72695	38	61	cellulase	cellulase	NOUN
afs-72695	38	62	,	,	PUNCT
afs-72695	38	63	0.5	0.5	NUM
afs-72695	38	64	%	%	NOUN
afs-72695	38	65	driselase	driselase	NOUN
afs-72695	38	66	,	,	PUNCT
afs-72695	38	67	0.1	0.1	NUM
afs-72695	38	68	%	%	NOUN
afs-72695	38	69	pectolyase	pectolyase	NOUN
afs-72695	38	70	y-23	y-23	PROPN
afs-72695	38	71	,	,	PUNCT
afs-72695	38	72	6.5	6.5	NUM
afs-72695	38	73	%	%	NOUN
afs-72695	38	74	glycerol	glycerol	NOUN
afs-72695	38	75	,	,	PUNCT
afs-72695	38	76	1.0	1.0	NUM
afs-72695	38	77	%	%	NOUN
afs-72695	38	78	cacl	cacl	X
afs-72695	38	79	„	„	PUNCT
afs-72695	38	80	0.1	0.1	NUM
afs-72695	38	81	%	%	NOUN
afs-72695	38	82	mgs04	mgs04	ADV
afs-72695	38	83	and	and	CCONJ
afs-72695	38	84	0.05	0.05	NUM
afs-72695	38	85	%	%	NOUN
afs-72695	38	86	kh	kh	PROPN
afs-72695	38	87	,	,	PUNCT
afs-72695	38	88	p0	p0	PROPN
afs-72695	38	89	4	4	NUM
afs-72695	38	90	,	,	PUNCT
afs-72695	38	91	3	3	X
afs-72695	38	92	)	)	PUNCT
afs-72695	38	93	lle	lle	NOUN
afs-72695	38	94	enzyme	enzyme	NOUN
afs-72695	38	95	mixture	mixture	NOUN
afs-72695	38	96	modified	modify	VERB
afs-72695	38	97	after	after	ADP
afs-72695	38	98	luhrs	luhrs	PROPN
afs-72695	38	99	and	and	CCONJ
afs-72695	38	100	lörz	lörz	PROPN
afs-72695	38	101	(	(	PUNCT
afs-72695	38	102	1988	1988	NUM
afs-72695	38	103	)	)	PUNCT
afs-72695	38	104	containing	contain	VERB
afs-72695	38	105	1	1	NUM
afs-72695	38	106	%	%	NOUN
afs-72695	38	107	onozuka	onozuka	NOUN
afs-72695	38	108	rs	rs	NOUN
afs-72695	38	109	cellulase	cellulase	NOUN
afs-72695	38	110	,	,	PUNCT
afs-72695	38	111	0.5	0.5	NUM
afs-72695	38	112	%	%	NOUN
afs-72695	38	113	macerozyme	macerozyme	NOUN
afs-72695	38	114	rlo	rlo	PROPN
afs-72695	38	115	,	,	PUNCT
afs-72695	38	116	0.1	0.1	NUM
afs-72695	38	117	%	%	NOUN
afs-72695	38	118	pectolyase	pectolyase	NOUN
afs-72695	38	119	y-23	y-23	NOUN
afs-72695	38	120	and	and	CCONJ
afs-72695	38	121	0.1	0.1	NUM
afs-72695	38	122	%	%	NOUN
afs-72695	38	123	casein	casein	NOUN
afs-72695	38	124	hydrolysate	hydrolysate	NOUN
afs-72695	38	125	.	.	PUNCT
afs-72695	39	1	the	the	DET
afs-72695	39	2	protoplasts	protoplast	NOUN
afs-72695	39	3	were	be	AUX
afs-72695	39	4	kept	keep	VERB
afs-72695	39	5	in	in	ADP
afs-72695	39	6	the	the	DET
afs-72695	39	7	enzyme	enzyme	NOUN
afs-72695	39	8	at	at	ADP
afs-72695	39	9	25	25	NUM
afs-72695	39	10	°	°	NOUN
afs-72695	39	11	c	c	NOUN
afs-72695	39	12	for	for	ADP
afs-72695	39	13	3	3	NUM
afs-72695	39	14	to	to	PART
afs-72695	39	15	6	6	NUM
afs-72695	39	16	hours	hour	NOUN
afs-72695	39	17	.	.	PUNCT
afs-72695	40	1	the	the	DET
afs-72695	40	2	protoplast	protoplast	NOUN
afs-72695	40	3	were	be	AUX
afs-72695	40	4	then	then	ADV
afs-72695	40	5	200	200	NUM
afs-72695	40	6	agricultural	agricultural	ADJ
afs-72695	40	7	science	science	NOUN
afs-72695	40	8	in	in	ADP
afs-72695	40	9	finland	finland	PROPN
afs-72695	40	10	3	3	NUM
afs-72695	40	11	(	(	PUNCT
afs-72695	40	12	1994	1994	NUM
afs-72695	40	13	)	)	PUNCT
afs-72695	40	14	agricultural	agricultural	ADJ
afs-72695	40	15	science	science	NOUN
afs-72695	40	16	in	in	ADP
afs-72695	40	17	finland	finland	PROPN
afs-72695	40	18	3	3	NUM
afs-72695	40	19	(	(	PUNCT
afs-72695	40	20	1994	1994	NUM
afs-72695	40	21	)	)	PUNCT
afs-72695	40	22	cleaned	clean	VERB
afs-72695	40	23	by	by	ADP
afs-72695	40	24	sieving	sieve	VERB
afs-72695	40	25	the	the	DET
afs-72695	40	26	mixture	mixture	NOUN
afs-72695	40	27	through	through	ADP
afs-72695	40	28	a	a	DET
afs-72695	40	29	plastic	plastic	ADJ
afs-72695	40	30	mesh	mesh	NOUN
afs-72695	40	31	with	with	ADP
afs-72695	40	32	pores	pore	NOUN
afs-72695	40	33	of	of	ADP
afs-72695	40	34	100	100	NUM
afs-72695	40	35	pm	pm	NOUN
afs-72695	40	36	in	in	ADP
afs-72695	40	37	diameter	diameter	NOUN
afs-72695	40	38	and	and	CCONJ
afs-72695	40	39	centrifuged	centrifuge	VERB
afs-72695	40	40	at	at	ADP
afs-72695	40	41	800	800	NUM
afs-72695	40	42	rpm	rpm	NOUN
afs-72695	40	43	for	for	ADP
afs-72695	40	44	5	5	NUM
afs-72695	40	45	min	min	NOUN
afs-72695	40	46	.	.	NOUN
afs-72695	40	47	protoplasts	protoplast	NOUN
afs-72695	40	48	were	be	AUX
afs-72695	40	49	resuspended	resuspend	VERB
afs-72695	40	50	in	in	ADP
afs-72695	40	51	2	2	NUM
afs-72695	40	52	ml	ml	NOUN
afs-72695	40	53	w	w	ADP
afs-72695	40	54	5	5	NUM
afs-72695	40	55	washing	washing	NOUN
afs-72695	40	56	solution	solution	NOUN
afs-72695	40	57	(	(	PUNCT
afs-72695	40	58	menczel	menczel	PROPN
afs-72695	40	59	et	et	PROPN
afs-72695	40	60	al	al	PROPN
afs-72695	40	61	.	.	PROPN
afs-72695	40	62	1981	1981	NUM
afs-72695	40	63	)	)	PUNCT
afs-72695	40	64	and	and	CCONJ
afs-72695	40	65	the	the	DET
afs-72695	40	66	suspension	suspension	NOUN
afs-72695	40	67	was	be	AUX
afs-72695	40	68	then	then	ADV
afs-72695	40	69	laid	lay	VERB
afs-72695	40	70	on	on	ADP
afs-72695	40	71	the	the	DET
afs-72695	40	72	top	top	NOUN
afs-72695	40	73	of	of	ADP
afs-72695	40	74	0.6	0.6	NUM
afs-72695	40	75	m	m	NOUN
afs-72695	40	76	sucrose	sucrose	VERB
afs-72695	40	77	solution	solution	NOUN
afs-72695	40	78	.	.	PUNCT
afs-72695	41	1	after	after	SCONJ
afs-72695	41	2	5	5	NUM
afs-72695	41	3	min	min	NOUN
afs-72695	41	4	of	of	ADP
afs-72695	41	5	centrifugation	centrifugation	NOUN
afs-72695	41	6	at	at	ADP
afs-72695	41	7	800	800	NUM
afs-72695	41	8	rpm	rpm	ADP
afs-72695	41	9	the	the	DET
afs-72695	41	10	protoplasts	protoplast	NOUN
afs-72695	41	11	were	be	AUX
afs-72695	41	12	collected	collect	VERB
afs-72695	41	13	from	from	ADP
afs-72695	41	14	the	the	DET
afs-72695	41	15	interphase	interphase	NOUN
afs-72695	41	16	.	.	PUNCT
afs-72695	42	1	the	the	DET
afs-72695	42	2	cleaning	cleaning	NOUN
afs-72695	42	3	was	be	AUX
afs-72695	42	4	finished	finish	VERB
afs-72695	42	5	by	by	ADP
afs-72695	42	6	washing	wash	VERB
afs-72695	42	7	the	the	DET
afs-72695	42	8	protoplasts	protoplast	NOUN
afs-72695	42	9	twice	twice	ADV
afs-72695	42	10	with	with	ADP
afs-72695	42	11	w	w	PROPN
afs-72695	42	12	5	5	NUM
afs-72695	42	13	solution	solution	NOUN
afs-72695	42	14	.	.	PUNCT
afs-72695	43	1	plasmids	plasmid	NOUN
afs-72695	43	2	plasmid	plasmid	ADJ
afs-72695	43	3	pactl	pactl	NOUN
afs-72695	43	4	-	-	PUNCT
afs-72695	43	5	f	f	PROPN
afs-72695	43	6	(	(	PUNCT
afs-72695	43	7	mcelroy	mcelroy	PROPN
afs-72695	43	8	et	et	PROPN
afs-72695	43	9	al	al	PROPN
afs-72695	43	10	.	.	PROPN
afs-72695	43	11	1991	1991	NUM
afs-72695	43	12	)	)	PUNCT
afs-72695	43	13	was	be	AUX
afs-72695	43	14	provided	provide	VERB
afs-72695	43	15	by	by	ADP
afs-72695	43	16	the	the	DET
afs-72695	43	17	courtesy	courtesy	NOUN
afs-72695	43	18	of	of	ADP
afs-72695	43	19	professor	professor	PROPN
afs-72695	43	20	ray	ray	PROPN
afs-72695	43	21	wu	wu	PROPN
afs-72695	43	22	,	,	PUNCT
afs-72695	43	23	cornell	cornell	PROPN
afs-72695	43	24	university	university	PROPN
afs-72695	43	25	,	,	PUNCT
afs-72695	43	26	ithaca	ithaca	PROPN
afs-72695	43	27	,	,	PUNCT
afs-72695	43	28	ny	ny	PROPN
afs-72695	43	29	.	.	PUNCT
afs-72695	44	1	this	this	DET
afs-72695	44	2	plasmid	plasmid	NOUN
afs-72695	44	3	includes	include	VERB
afs-72695	44	4	therice	therice	NOUN
afs-72695	44	5	actin	actin	NOUN
afs-72695	44	6	1	1	NUM
afs-72695	44	7	gene	gene	NOUN
afs-72695	44	8	5	5	NUM
afs-72695	44	9	’	'	PUNCT
afs-72695	44	10	regulatory	regulatory	ADJ
afs-72695	44	11	elements	element	NOUN
afs-72695	44	12	linked	link	VERB
afs-72695	44	13	to	to	ADP
afs-72695	44	14	the	the	DET
afs-72695	44	15	gus	gus	NOUN
afs-72695	44	16	gene	gene	NOUN
afs-72695	44	17	coding	code	VERB
afs-72695	44	18	sequences	sequence	NOUN
afs-72695	44	19	(	(	PUNCT
afs-72695	44	20	synonym	synonym	NOUN
afs-72695	44	21	uida	uida	PROPN
afs-72695	44	22	,	,	PUNCT
afs-72695	44	23	codes	code	NOUN
afs-72695	44	24	for	for	ADP
afs-72695	44	25	(	(	PUNCT
afs-72695	44	26	3	3	NUM
afs-72695	44	27	-	-	PUNCT
afs-72695	44	28	glucuronidase	glucuronidase	NOUN
afs-72695	44	29	enzyme	enzyme	NOUN
afs-72695	44	30	)	)	PUNCT
afs-72695	44	31	from	from	ADP
afs-72695	44	32	e.	e.	PROPN
afs-72695	44	33	coli	coli	PROPN
afs-72695	44	34	and	and	CCONJ
afs-72695	44	35	the	the	DET
afs-72695	44	36	nos	nos	X
afs-72695	44	37	polyadenylation	polyadenylation	NOUN
afs-72695	44	38	site	site	NOUN
afs-72695	44	39	from	from	ADP
afs-72695	44	40	agrobacterium	agrobacterium	NOUN
afs-72695	44	41	tumefaciens	tumefacien	NOUN
afs-72695	44	42	.	.	PUNCT
afs-72695	45	1	transformation	transformation	NOUN
afs-72695	45	2	of	of	ADP
afs-72695	45	3	protoplasts	protoplast	NOUN
afs-72695	45	4	the	the	DET
afs-72695	45	5	number	number	NOUN
afs-72695	45	6	of	of	ADP
afs-72695	45	7	protoplasts	protoplast	NOUN
afs-72695	45	8	suspended	suspend	VERB
afs-72695	45	9	in	in	ADP
afs-72695	45	10	the	the	DET
afs-72695	45	11	w5	w5	PROPN
afs-72695	45	12	solution	solution	NOUN
afs-72695	45	13	was	be	AUX
afs-72695	45	14	estimated	estimate	VERB
afs-72695	45	15	in	in	ADP
afs-72695	45	16	a	a	DET
afs-72695	45	17	buerker	buerker	NOUN
afs-72695	45	18	chamber	chamber	NOUN
afs-72695	45	19	and	and	CCONJ
afs-72695	45	20	then	then	ADV
afs-72695	45	21	divided	divide	VERB
afs-72695	45	22	into	into	ADP
afs-72695	45	23	1	1	NUM
afs-72695	45	24	x	x	SYM
afs-72695	45	25	106	106	NUM
afs-72695	45	26	aliquots	aliquot	NOUN
afs-72695	45	27	in	in	ADP
afs-72695	45	28	plastic	plastic	ADJ
afs-72695	45	29	wassermann	wassermann	PROPN
afs-72695	45	30	tubes	tube	NOUN
afs-72695	45	31	.	.	PUNCT
afs-72695	46	1	after	after	SCONJ
afs-72695	46	2	centrifugation	centrifugation	NOUN
afs-72695	46	3	at	at	ADP
afs-72695	46	4	800	800	NUM
afs-72695	46	5	rpm	rpm	NOUN
afs-72695	46	6	for	for	ADP
afs-72695	46	7	5	5	NUM
afs-72695	46	8	min	min	NOUN
afs-72695	46	9	the	the	DET
afs-72695	46	10	supernatant	supernatant	NOUN
afs-72695	46	11	was	be	AUX
afs-72695	46	12	removed	remove	VERB
afs-72695	46	13	and	and	CCONJ
afs-72695	46	14	the	the	DET
afs-72695	46	15	pellet	pellet	NOUN
afs-72695	46	16	was	be	AUX
afs-72695	46	17	resuspended	resuspend	VERB
afs-72695	46	18	in	in	ADP
afs-72695	46	19	1	1	NUM
afs-72695	46	20	ml	ml	NOUN
afs-72695	46	21	mgma	mgma	NOUN
afs-72695	46	22	transformation	transformation	NOUN
afs-72695	46	23	buffer	buffer	NOUN
afs-72695	46	24	(	(	PUNCT
afs-72695	46	25	zhang	zhang	PROPN
afs-72695	46	26	et	et	PROPN
afs-72695	46	27	al	al	PROPN
afs-72695	46	28	.	.	PROPN
afs-72695	46	29	1991	1991	NUM
afs-72695	46	30	)	)	PUNCT
afs-72695	46	31	.	.	PUNCT
afs-72695	47	1	twenty	twenty	NUM
afs-72695	47	2	mg	mg	PROPN
afs-72695	47	3	of	of	ADP
afs-72695	47	4	uncut	uncut	ADJ
afs-72695	47	5	pactl	pactl	NOUN
afs-72695	47	6	-	-	PUNCT
afs-72695	47	7	f	f	PROPN
afs-72695	47	8	plasmid	plasmid	NOUN
afs-72695	47	9	and	and	CCONJ
afs-72695	47	10	100	100	NUM
afs-72695	47	11	pg	pg	NOUN
afs-72695	47	12	of	of	ADP
afs-72695	47	13	herring	herre	VERB
afs-72695	47	14	testis	testis	NOUN
afs-72695	47	15	dna	dna	PROPN
afs-72695	47	16	(	(	PUNCT
afs-72695	47	17	sigma	sigma	PROPN
afs-72695	47	18	,	,	PUNCT
afs-72695	47	19	ruptured	rupture	VERB
afs-72695	47	20	by	by	ADP
afs-72695	47	21	sonication	sonication	NOUN
afs-72695	47	22	)	)	PUNCT
afs-72695	47	23	were	be	AUX
afs-72695	47	24	added	add	VERB
afs-72695	47	25	to	to	ADP
afs-72695	47	26	the	the	DET
afs-72695	47	27	protoplast	protoplast	NOUN
afs-72695	47	28	suspension	suspension	NOUN
afs-72695	47	29	.	.	PUNCT
afs-72695	48	1	after	after	ADP
afs-72695	48	2	5	5	NUM
afs-72695	48	3	min	min	NOUN
afs-72695	48	4	of	of	ADP
afs-72695	48	5	gentle	gentle	ADJ
afs-72695	48	6	handvortexing	handvortexing	NOUN
afs-72695	48	7	1	1	NUM
afs-72695	48	8	ml	ml	NOUN
afs-72695	48	9	of	of	ADP
afs-72695	48	10	30	30	NUM
afs-72695	48	11	%	%	NOUN
afs-72695	48	12	solution	solution	NOUN
afs-72695	48	13	of	of	ADP
afs-72695	48	14	peg	peg	NOUN
afs-72695	48	15	(	(	PUNCT
afs-72695	48	16	polyethylen	polyethylen	ADJ
afs-72695	48	17	glykol	glykol	NOUN
afs-72695	48	18	,	,	PUNCT
afs-72695	48	19	fw	fw	PROPN
afs-72695	48	20	3450	3450	NUM
afs-72695	48	21	,	,	PUNCT
afs-72695	48	22	sigma	sigma	PROPN
afs-72695	48	23	)	)	PUNCT
afs-72695	48	24	solution	solution	NOUN
afs-72695	48	25	was	be	AUX
afs-72695	48	26	added	add	VERB
afs-72695	48	27	(	(	PUNCT
afs-72695	48	28	zhang	zhang	X
afs-72695	48	29	et	et	PROPN
afs-72695	48	30	al	al	PROPN
afs-72695	48	31	.	.	PROPN
afs-72695	48	32	1991	1991	NUM
afs-72695	48	33	)	)	PUNCT
afs-72695	48	34	.	.	PUNCT
afs-72695	49	1	the	the	DET
afs-72695	49	2	protoplasts	protoplast	NOUN
afs-72695	49	3	were	be	AUX
afs-72695	49	4	incubated	incubate	VERB
afs-72695	49	5	with	with	ADP
afs-72695	49	6	the	the	DET
afs-72695	49	7	peg	peg	NOUN
afs-72695	49	8	for	for	ADP
afs-72695	49	9	28	28	NUM
afs-72695	49	10	min	min	NOUN
afs-72695	49	11	.	.	PROPN
afs-72695	49	12	during	during	ADP
afs-72695	49	13	this	this	DET
afs-72695	49	14	time	time	NOUN
afs-72695	49	15	the	the	DET
afs-72695	49	16	suspension	suspension	NOUN
afs-72695	49	17	was	be	AUX
afs-72695	49	18	gently	gently	ADV
afs-72695	49	19	shaken	shake	VERB
afs-72695	49	20	by	by	ADP
afs-72695	49	21	hand	hand	NOUN
afs-72695	49	22	in	in	ADP
afs-72695	49	23	every	every	DET
afs-72695	49	24	5	5	NUM
afs-72695	49	25	min	min	NOUN
afs-72695	49	26	.	.	PROPN
afs-72695	50	1	at	at	ADP
afs-72695	50	2	the	the	DET
afs-72695	50	3	end	end	NOUN
afs-72695	50	4	of	of	ADP
afs-72695	50	5	the	the	DET
afs-72695	50	6	incubation	incubation	NOUN
afs-72695	50	7	time	time	NOUN
afs-72695	50	8	the	the	DET
afs-72695	50	9	mixture	mixture	NOUN
afs-72695	50	10	was	be	AUX
afs-72695	50	11	slowly	slowly	ADV
afs-72695	50	12	diluted	dilute	VERB
afs-72695	50	13	with	with	ADP
afs-72695	50	14	w	w	PROPN
afs-72695	50	15	5	5	NUM
afs-72695	50	16	solution	solution	NOUN
afs-72695	50	17	up	up	ADP
afs-72695	50	18	to	to	PART
afs-72695	50	19	10	10	NUM
afs-72695	50	20	ml	ml	NOUN
afs-72695	50	21	within	within	ADP
afs-72695	50	22	5	5	NUM
afs-72695	50	23	min	min	NOUN
afs-72695	50	24	,	,	PUNCT
afs-72695	50	25	adding	add	VERB
afs-72695	50	26	the	the	DET
afs-72695	50	27	w	w	PROPN
afs-72695	50	28	5	5	NUM
afs-72695	50	29	solution	solution	NOUN
afs-72695	50	30	drop	drop	NOUN
afs-72695	50	31	by	by	ADP
afs-72695	50	32	drop	drop	NOUN
afs-72695	50	33	and	and	CCONJ
afs-72695	50	34	mixing	mixing	NOUN
afs-72695	50	35	.	.	PUNCT
afs-72695	51	1	the	the	DET
afs-72695	51	2	diluted	dilute	VERB
afs-72695	51	3	mixture	mixture	NOUN
afs-72695	51	4	then	then	ADV
afs-72695	51	5	was	be	AUX
afs-72695	51	6	centrifuged	centrifuge	VERB
afs-72695	51	7	and	and	CCONJ
afs-72695	51	8	the	the	DET
afs-72695	51	9	protoplasts	protoplast	NOUN
afs-72695	51	10	washed	wash	VERB
afs-72695	51	11	twice	twice	ADV
afs-72695	51	12	in	in	ADP
afs-72695	51	13	w	w	PROPN
afs-72695	51	14	5	5	NUM
afs-72695	51	15	solution	solution	NOUN
afs-72695	51	16	.	.	PUNCT
afs-72695	52	1	culture	culture	NOUN
afs-72695	52	2	of	of	ADP
afs-72695	52	3	protoplasts	protoplast	NOUN
afs-72695	52	4	the	the	DET
afs-72695	52	5	washed	washed	NOUN
afs-72695	52	6	and	and	CCONJ
afs-72695	52	7	pelleted	pellete	VERB
afs-72695	52	8	protoplasts	protoplast	NOUN
afs-72695	52	9	were	be	AUX
afs-72695	52	10	resuspended	resuspend	VERB
afs-72695	52	11	in	in	ADP
afs-72695	52	12	1	1	NUM
afs-72695	52	13	ml	ml	NOUN
afs-72695	52	14	of	of	ADP
afs-72695	52	15	kpr	kpr	PROPN
afs-72695	52	16	protoplast	protoplast	PROPN
afs-72695	52	17	culture	culture	NOUN
afs-72695	52	18	medium	medium	NOUN
afs-72695	52	19	(	(	PUNCT
afs-72695	52	20	thompson	thompson	PROPN
afs-72695	52	21	et	et	PROPN
afs-72695	52	22	al	al	PROPN
afs-72695	52	23	.	.	PROPN
afs-72695	52	24	1986	1986	NUM
afs-72695	52	25	)	)	PUNCT
afs-72695	52	26	and	and	CCONJ
afs-72695	52	27	placed	place	VERB
afs-72695	52	28	into	into	ADP
afs-72695	52	29	a	a	DET
afs-72695	52	30	24	24	NUM
afs-72695	52	31	well	well	ADV
afs-72695	52	32	sterile	sterile	ADJ
afs-72695	52	33	plate	plate	NOUN
afs-72695	52	34	(	(	PUNCT
afs-72695	52	35	corning	corning	NOUN
afs-72695	52	36	)	)	PUNCT
afs-72695	52	37	so	so	SCONJ
afs-72695	52	38	that	that	SCONJ
afs-72695	52	39	250	250	NUM
afs-72695	52	40	pi	pi	NOUN
afs-72695	52	41	of	of	ADP
afs-72695	52	42	suspension	suspension	NOUN
afs-72695	52	43	was	be	AUX
afs-72695	52	44	transferred	transfer	VERB
afs-72695	52	45	into	into	ADP
afs-72695	52	46	each	each	DET
afs-72695	52	47	well	well	NOUN
afs-72695	52	48	.	.	PUNCT
afs-72695	53	1	the	the	DET
afs-72695	53	2	plate	plate	NOUN
afs-72695	53	3	was	be	AUX
afs-72695	53	4	sealed	seal	VERB
afs-72695	53	5	with	with	ADP
afs-72695	53	6	parafilm	parafilm	NOUN
afs-72695	53	7	and	and	CCONJ
afs-72695	53	8	incubated	incubate	VERB
afs-72695	53	9	at	at	ADP
afs-72695	53	10	25	25	NUM
afs-72695	53	11	°	°	NOUN
afs-72695	53	12	c	c	NOUN
afs-72695	53	13	in	in	ADP
afs-72695	53	14	dark	dark	NOUN
afs-72695	53	15	until	until	SCONJ
afs-72695	53	16	the	the	DET
afs-72695	53	17	gus	gus	NOUN
afs-72695	53	18	assay	assay	PROPN
afs-72695	53	19	.	.	PUNCT
afs-72695	54	1	detection	detection	NOUN
afs-72695	54	2	of	of	ADP
afs-72695	54	3	transient	transient	ADJ
afs-72695	54	4	expression	expression	NOUN
afs-72695	54	5	ofgus	ofgus	ADJ
afs-72695	54	6	gene	gene	NOUN
afs-72695	54	7	samples	sample	NOUN
afs-72695	54	8	of	of	ADP
afs-72695	54	9	control	control	NOUN
afs-72695	54	10	and	and	CCONJ
afs-72695	54	11	transformed	transform	VERB
afs-72695	54	12	protoplast	protoplast	NOUN
afs-72695	54	13	cultures	culture	NOUN
afs-72695	54	14	were	be	AUX
afs-72695	54	15	placed	place	VERB
afs-72695	54	16	into	into	ADP
afs-72695	54	17	a	a	DET
afs-72695	54	18	96	96	NUM
afs-72695	54	19	well	well	ADV
afs-72695	54	20	sterile	sterile	ADJ
afs-72695	54	21	plate	plate	NOUN
afs-72695	54	22	(	(	PUNCT
afs-72695	54	23	corning	corning	NOUN
afs-72695	54	24	)	)	PUNCT
afs-72695	54	25	,	,	PUNCT
afs-72695	54	26	100	100	NUM
afs-72695	54	27	pi	pi	NOUN
afs-72695	54	28	of	of	ADP
afs-72695	54	29	culture	culture	NOUN
afs-72695	54	30	into	into	ADP
afs-72695	54	31	each	each	DET
afs-72695	54	32	well	well	NOUN
afs-72695	54	33	.	.	PUNCT
afs-72695	55	1	forty	forty	NUM
afs-72695	55	2	pi	pi	NOUN
afs-72695	55	3	of	of	ADP
afs-72695	55	4	x	x	ADJ
afs-72695	55	5	-	-	ADJ
afs-72695	55	6	gluc	gluc	ADJ
afs-72695	55	7	staining	stain	VERB
afs-72695	55	8	solution	solution	NOUN
afs-72695	55	9	(	(	PUNCT
afs-72695	55	10	jefferson	jefferson	PROPN
afs-72695	55	11	et	et	PROPN
afs-72695	55	12	al	al	PROPN
afs-72695	55	13	.	.	PROPN
afs-72695	55	14	1986	1986	NUM
afs-72695	55	15	,	,	PUNCT
afs-72695	55	16	jefferson	jefferson	PROPN
afs-72695	55	17	1987	1987	NUM
afs-72695	55	18	)	)	PUNCT
afs-72695	55	19	was	be	AUX
afs-72695	55	20	added	add	VERB
afs-72695	55	21	into	into	ADP
afs-72695	55	22	each	each	DET
afs-72695	55	23	well	well	NOUN
afs-72695	55	24	.	.	PUNCT
afs-72695	56	1	the	the	DET
afs-72695	56	2	plates	plate	NOUN
afs-72695	56	3	were	be	AUX
afs-72695	56	4	incubated	incubate	VERB
afs-72695	56	5	at	at	ADP
afs-72695	56	6	29	29	NUM
afs-72695	56	7	°	°	ADP
afs-72695	56	8	c	c	NOUN
afs-72695	56	9	for	for	ADP
afs-72695	56	10	12	12	NUM
afs-72695	56	11	hours	hour	NOUN
afs-72695	56	12	before	before	SCONJ
afs-72695	56	13	the	the	DET
afs-72695	56	14	visual	visual	ADJ
afs-72695	56	15	counting	counting	NOUN
afs-72695	56	16	of	of	ADP
afs-72695	56	17	the	the	DET
afs-72695	56	18	cells	cell	NOUN
afs-72695	56	19	showing	show	VERB
afs-72695	56	20	transient	transient	ADJ
afs-72695	56	21	expression	expression	NOUN
afs-72695	56	22	was	be	AUX
afs-72695	56	23	carried	carry	VERB
afs-72695	56	24	out	out	ADP
afs-72695	56	25	.	.	PUNCT
afs-72695	57	1	results	result	NOUN
afs-72695	57	2	and	and	CCONJ
afs-72695	57	3	discussion	discussion	NOUN
afs-72695	57	4	protoplast	protoplast	NOUN
afs-72695	57	5	isolation	isolation	NOUN
afs-72695	57	6	successful	successful	ADJ
afs-72695	57	7	protoplast	protoplast	NOUN
afs-72695	57	8	isolation	isolation	NOUN
afs-72695	57	9	was	be	AUX
afs-72695	57	10	achieved	achieve	VERB
afs-72695	57	11	from	from	ADP
afs-72695	57	12	the	the	DET
afs-72695	57	13	mature	mature	ADJ
afs-72695	57	14	scutellum	scutellum	NOUN
afs-72695	57	15	-	-	PUNCT
afs-72695	57	16	embryo	embryo	NOUN
afs-72695	57	17	complexes	complex	NOUN
afs-72695	57	18	of	of	ADP
afs-72695	57	19	each	each	PRON
afs-72695	57	20	of	of	ADP
afs-72695	57	21	the	the	DET
afs-72695	57	22	four	four	NUM
afs-72695	57	23	genotypes	genotype	NOUN
afs-72695	57	24	used	use	VERB
afs-72695	57	25	in	in	ADP
afs-72695	57	26	this	this	DET
afs-72695	57	27	study	study	NOUN
afs-72695	57	28	(	(	PUNCT
afs-72695	57	29	fig	fig	NOUN
afs-72695	57	30	.	.	PUNCT
afs-72695	58	1	2	2	NUM
afs-72695	58	2	)	)	PUNCT
afs-72695	58	3	.	.	PUNCT
afs-72695	59	1	in	in	ADP
afs-72695	59	2	our	our	PRON
afs-72695	59	3	first	first	ADJ
afs-72695	59	4	experiments	experiment	NOUN
afs-72695	59	5	three	three	NUM
afs-72695	59	6	different	different	ADJ
afs-72695	59	7	digestion	digestion	NOUN
afs-72695	59	8	enzyme	enzyme	NOUN
afs-72695	59	9	mixtures	mixture	NOUN
afs-72695	59	10	were	be	AUX
afs-72695	59	11	tested	test	VERB
afs-72695	59	12	,	,	PUNCT
afs-72695	59	13	each	each	PRON
afs-72695	59	14	of	of	ADP
afs-72695	59	15	which	which	PRON
afs-72695	59	16	were	be	AUX
afs-72695	59	17	modified	modify	VERB
afs-72695	59	18	from	from	ADP
afs-72695	59	19	the	the	DET
afs-72695	59	20	original	original	ADJ
afs-72695	59	21	references	reference	NOUN
afs-72695	59	22	.	.	PUNCT
afs-72695	60	1	the	the	DET
afs-72695	60	2	results	result	NOUN
afs-72695	60	3	showed	show	VERB
afs-72695	60	4	the	the	DET
afs-72695	60	5	superiority	superiority	NOUN
afs-72695	60	6	of	of	ADP
afs-72695	60	7	the	the	DET
afs-72695	60	8	je	je	X
afs-72695	60	9	enzyme	enzyme	NOUN
afs-72695	60	10	mixture	mixture	NOUN
afs-72695	60	11	in	in	ADP
afs-72695	60	12	protoplast	protoplast	NOUN
afs-72695	60	13	yielding	yield	VERB
afs-72695	60	14	(	(	PUNCT
afs-72695	60	15	table	table	NOUN
afs-72695	60	16	1	1	NUM
afs-72695	60	17	)	)	PUNCT
afs-72695	60	18	.	.	PUNCT
afs-72695	61	1	consetable	consetable	ADJ
afs-72695	61	2	1	1	NUM
afs-72695	61	3	.	.	PUNCT
afs-72695	61	4	differencies	differencie	NOUN
afs-72695	61	5	in	in	ADP
afs-72695	61	6	the	the	DET
afs-72695	61	7	effectiveness	effectiveness	NOUN
afs-72695	61	8	of	of	ADP
afs-72695	61	9	the	the	DET
afs-72695	61	10	three	three	NUM
afs-72695	61	11	enzyme	enzyme	NOUN
afs-72695	61	12	mixtures	mixture	NOUN
afs-72695	61	13	applied	apply	VERB
afs-72695	61	14	to	to	ADP
afs-72695	61	15	protoplast	protoplast	NOUN
afs-72695	61	16	isolation	isolation	NOUN
afs-72695	61	17	from	from	ADP
afs-72695	61	18	embryo	embryo	NOUN
afs-72695	61	19	-	-	PUNCT
afs-72695	61	20	scutellum	scutellum	NOUN
afs-72695	61	21	complexes	complex	NOUN
afs-72695	61	22	of	of	ADP
afs-72695	61	23	barley	barley	NOUN
afs-72695	61	24	‘	'	PUNCT
afs-72695	61	25	kymppi	kymppi	X
afs-72695	61	26	’	'	PUNCT
afs-72695	61	27	.	.	PUNCT
afs-72695	62	1	yield	yield	NOUN
afs-72695	62	2	of	of	ADP
afs-72695	62	3	protoplasts	protoplast	NOUN
afs-72695	62	4	/enzyme	/enzyme	PUNCT
afs-72695	62	5	mixture	mixture	NOUN
afs-72695	62	6	100	100	NUM
afs-72695	62	7	embryos	embryo	NOUN
afs-72695	62	8	2.2	2.2	NUM
afs-72695	62	9	x	x	SYM
afs-72695	62	10	106	106	NUM
afs-72695	62	11	1.2	1.2	NUM
afs-72695	62	12	x	x	SYM
afs-72695	62	13	10	10	NUM
afs-72695	62	14	"	"	NUM
afs-72695	62	15	0.2	0.2	NUM
afs-72695	62	16	x	x	SYM
afs-72695	62	17	106	106	NUM
afs-72695	62	18	je	je	NOUN
afs-72695	62	19	enzyme	enzyme	NOUN
afs-72695	62	20	mixture	mixture	NOUN
afs-72695	62	21	1	1	NUM
afs-72695	62	22	ke	ke	NOUN
afs-72695	62	23	enzyme	enzyme	NOUN
afs-72695	62	24	mixture2	mixture2	ADJ
afs-72695	62	25	lee	lee	PROPN
afs-72695	62	26	enzyme	enzyme	NOUN
afs-72695	62	27	mixture	mixture	NOUN
afs-72695	62	28	3	3	NUM
afs-72695	62	29	1	1	NUM
afs-72695	62	30	modified	modify	VERB
afs-72695	62	31	after	after	ADP
afs-72695	62	32	junker	junker	PROPN
afs-72695	62	33	et	et	PROPN
afs-72695	62	34	al	al	PROPN
afs-72695	62	35	.	.	PROPN
afs-72695	62	36	1987	1987	NUM
afs-72695	62	37	.	.	PUNCT
afs-72695	63	1	2	2	NUM
afs-72695	63	2	modified	modify	VERB
afs-72695	63	3	after	after	ADP
afs-72695	63	4	ahmed	ahmed	PROPN
afs-72695	63	5	and	and	CCONJ
afs-72695	63	6	sagi	sagi	PROPN
afs-72695	63	7	1993	1993	NUM
afs-72695	63	8	.	.	PUNCT
afs-72695	64	1	3	3	NUM
afs-72695	64	2	modified	modify	VERB
afs-72695	64	3	after	after	ADP
afs-72695	64	4	luhrs	luhrs	NOUN
afs-72695	64	5	and	and	CCONJ
afs-72695	64	6	lorz	lorz	ADJ
afs-72695	64	7	1988	1988	NUM
afs-72695	64	8	.	.	PUNCT
afs-72695	65	1	201	201	NUM
afs-72695	65	2	research	research	NOUN
afs-72695	65	3	note	note	VERB
afs-72695	65	4	agricultural	agricultural	ADJ
afs-72695	65	5	science	science	NOUN
afs-72695	65	6	in	in	ADP
afs-72695	65	7	finland	finland	PROPN
afs-72695	65	8	3	3	NUM
afs-72695	65	9	(	(	PUNCT
afs-72695	65	10	1994	1994	NUM
afs-72695	65	11	)	)	PUNCT
afs-72695	65	12	table	table	NOUN
afs-72695	65	13	2	2	NUM
afs-72695	65	14	,	,	PUNCT
afs-72695	65	15	average	average	ADJ
afs-72695	65	16	yields	yield	NOUN
afs-72695	65	17	of	of	ADP
afs-72695	65	18	protoplasts	protoplast	NOUN
afs-72695	65	19	of	of	ADP
afs-72695	65	20	the	the	DET
afs-72695	65	21	four	four	NUM
afs-72695	65	22	different	different	ADJ
afs-72695	65	23	barley	barley	NOUN
afs-72695	65	24	cultivars	cultivar	NOUN
afs-72695	65	25	kymppi	kymppi	PROPN
afs-72695	65	26	,	,	PUNCT
afs-72695	65	27	prisma	prisma	PROPN
afs-72695	65	28	,	,	PUNCT
afs-72695	65	29	pohto	pohto	NOUN
afs-72695	65	30	and	and	CCONJ
afs-72695	65	31	igri	igri	NOUN
afs-72695	65	32	.	.	PUNCT
afs-72695	66	1	digestion	digestion	NOUN
afs-72695	66	2	was	be	AUX
afs-72695	66	3	performed	perform	VERB
afs-72695	66	4	by	by	ADP
afs-72695	66	5	the	the	DET
afs-72695	66	6	je	je	PROPN
afs-72695	66	7	enzyme	enzyme	NOUN
afs-72695	66	8	mixture	mixture	NOUN
afs-72695	66	9	'	'	PUNCT
afs-72695	66	10	.	.	PUNCT
afs-72695	67	1	number	number	NOUN
afs-72695	67	2	of	of	ADP
afs-72695	67	3	protoplasts	protoplast	NOUN
afs-72695	67	4	/name	/name	PUNCT
afs-72695	67	5	of	of	ADP
afs-72695	67	6	genotype	genotype	NOUN
afs-72695	67	7	100	100	NUM
afs-72695	67	8	embryos	embryo	NOUN
afs-72695	67	9	2.2	2.2	NUM
afs-72695	67	10	x	x	SYM
afs-72695	67	11	i06	i06	VERB
afs-72695	67	12	4.1	4.1	NUM
afs-72695	67	13	x	x	SYM
afs-72695	67	14	106	106	NUM
afs-72695	67	15	2.5	2.5	NUM
afs-72695	67	16	x	x	SYM
afs-72695	67	17	106	106	NUM
afs-72695	67	18	0.6	0.6	NUM
afs-72695	67	19	x	x	SYM
afs-72695	67	20	106	106	NUM
afs-72695	67	21	kymppi	kymppi	PROPN
afs-72695	67	22	prisma	prisma	PROPN
afs-72695	67	23	pohto	pohto	NOUN
afs-72695	67	24	igri	igri	VERB
afs-72695	67	25	1	1	NUM
afs-72695	67	26	modified	modify	VERB
afs-72695	67	27	after	after	ADP
afs-72695	67	28	junker	junker	PROPN
afs-72695	67	29	et	et	PROPN
afs-72695	67	30	al	al	PROPN
afs-72695	67	31	.	.	PROPN
afs-72695	67	32	1987	1987	NUM
afs-72695	67	33	.	.	PUNCT
afs-72695	68	1	quently	quently	ADV
afs-72695	68	2	,	,	PUNCT
afs-72695	68	3	this	this	DET
afs-72695	68	4	enzyme	enzyme	NOUN
afs-72695	68	5	mixture	mixture	NOUN
afs-72695	68	6	was	be	AUX
afs-72695	68	7	chosen	choose	VERB
afs-72695	68	8	for	for	ADP
afs-72695	68	9	the	the	DET
afs-72695	68	10	later	later	ADJ
afs-72695	68	11	experiments	experiment	NOUN
afs-72695	68	12	.	.	PUNCT
afs-72695	69	1	the	the	DET
afs-72695	69	2	four	four	NUM
afs-72695	69	3	barley	barley	NOUN
afs-72695	69	4	genotypes	genotype	NOUN
afs-72695	69	5	tested	test	VERB
afs-72695	69	6	in	in	ADP
afs-72695	69	7	this	this	DET
afs-72695	69	8	study	study	NOUN
afs-72695	69	9	showed	show	VERB
afs-72695	69	10	differences	difference	NOUN
afs-72695	69	11	in	in	ADP
afs-72695	69	12	protoplast	protoplast	NOUN
afs-72695	69	13	yielding	yield	VERB
afs-72695	69	14	and	and	CCONJ
afs-72695	69	15	characteristics	characteristic	NOUN
afs-72695	69	16	.	.	PUNCT
afs-72695	70	1	in	in	ADP
afs-72695	70	2	the	the	DET
afs-72695	70	3	digestion	digestion	NOUN
afs-72695	70	4	experiments	experiment	NOUN
afs-72695	70	5	as	as	ADV
afs-72695	70	6	much	much	ADV
afs-72695	70	7	as	as	ADP
afs-72695	70	8	x	x	SYM
afs-72695	70	9	106	106	NUM
afs-72695	70	10	protoplasts	protoplast	NOUN
afs-72695	70	11	per	per	ADP
afs-72695	70	12	100	100	NUM
afs-72695	70	13	dissected	dissect	VERB
afs-72695	70	14	embryo	embryo	NOUN
afs-72695	70	15	-	-	PUNCT
afs-72695	70	16	scutellum	scutellum	NOUN
afs-72695	70	17	complexes	complex	NOUN
afs-72695	70	18	were	be	AUX
afs-72695	70	19	obtained	obtain	VERB
afs-72695	70	20	.	.	PUNCT
afs-72695	71	1	the	the	DET
afs-72695	71	2	protoplast	protoplast	NOUN
afs-72695	71	3	yield	yield	NOUN
afs-72695	71	4	seemed	seem	VERB
afs-72695	71	5	to	to	PART
afs-72695	71	6	be	be	AUX
afs-72695	71	7	dependent	dependent	ADJ
afs-72695	71	8	on	on	ADP
afs-72695	71	9	the	the	DET
afs-72695	71	10	genotype	genotype	NOUN
afs-72695	71	11	and	and	CCONJ
afs-72695	71	12	the	the	DET
afs-72695	71	13	enzyme	enzyme	NOUN
afs-72695	71	14	mixture	mixture	NOUN
afs-72695	71	15	used	use	VERB
afs-72695	71	16	(	(	PUNCT
afs-72695	71	17	table	table	NOUN
afs-72695	71	18	2	2	NUM
afs-72695	71	19	)	)	PUNCT
afs-72695	71	20	.	.	PUNCT
afs-72695	72	1	the	the	DET
afs-72695	72	2	genotypes	genotype	NOUN
afs-72695	72	3	differed	differ	VERB
afs-72695	72	4	also	also	ADV
afs-72695	72	5	in	in	ADP
afs-72695	72	6	the	the	DET
afs-72695	72	7	size	size	NOUN
afs-72695	72	8	of	of	ADP
afs-72695	72	9	released	release	VERB
afs-72695	72	10	protoplasts	protoplast	NOUN
afs-72695	72	11	.	.	PUNCT
afs-72695	73	1	when	when	SCONJ
afs-72695	73	2	using	use	VERB
afs-72695	73	3	the	the	DET
afs-72695	73	4	je	je	X
afs-72695	73	5	enzyme	enzyme	NOUN
afs-72695	73	6	digestion	digestion	NOUN
afs-72695	73	7	,	,	PUNCT
afs-72695	73	8	pohto	pohto	NOUN
afs-72695	73	9	provided	provide	VERB
afs-72695	73	10	bigger	big	ADJ
afs-72695	73	11	protoplasts	protoplast	NOUN
afs-72695	73	12	of	of	ADP
afs-72695	73	13	50	50	NUM
afs-72695	73	14	-	-	SYM
afs-72695	73	15	80	80	NUM
afs-72695	73	16	|im	|im	NOUN
afs-72695	73	17	in	in	ADP
afs-72695	73	18	diameter	diameter	NOUN
afs-72695	73	19	than	than	ADP
afs-72695	73	20	any	any	PRON
afs-72695	73	21	of	of	ADP
afs-72695	73	22	the	the	DET
afs-72695	73	23	other	other	ADJ
afs-72695	73	24	three	three	NUM
afs-72695	73	25	cultivars	cultivar	NOUN
afs-72695	73	26	(	(	PUNCT
afs-72695	73	27	20	20	NUM
afs-72695	73	28	-	-	SYM
afs-72695	73	29	30	30	NUM
afs-72695	73	30	(	(	PUNCT
afs-72695	73	31	xm	xm	PROPN
afs-72695	73	32	in	in	ADP
afs-72695	73	33	diameter	diameter	NOUN
afs-72695	73	34	)	)	PUNCT
afs-72695	73	35	.	.	PUNCT
afs-72695	74	1	during	during	ADP
afs-72695	74	2	the	the	DET
afs-72695	74	3	digestion	digestion	NOUN
afs-72695	74	4	of	of	ADP
afs-72695	74	5	embryos	embryo	NOUN
afs-72695	74	6	from	from	ADP
afs-72695	74	7	the	the	DET
afs-72695	74	8	mature	mature	ADJ
afs-72695	74	9	embryo	embryo	NOUN
afs-72695	74	10	-	-	PUNCT
afs-72695	74	11	scutellum	scutellum	NOUN
afs-72695	74	12	complexes	complex	NOUN
afs-72695	74	13	it	it	PRON
afs-72695	74	14	was	be	AUX
afs-72695	74	15	visually	visually	ADV
afs-72695	74	16	estimated	estimate	VERB
afs-72695	74	17	that	that	SCONJ
afs-72695	74	18	about	about	ADV
afs-72695	74	19	80	80	NUM
afs-72695	74	20	%	%	NOUN
afs-72695	74	21	of	of	ADP
afs-72695	74	22	the	the	DET
afs-72695	74	23	protoplasts	protoplast	NOUN
afs-72695	74	24	were	be	AUX
afs-72695	74	25	released	release	VERB
afs-72695	74	26	from	from	ADP
afs-72695	74	27	the	the	DET
afs-72695	74	28	scutellar	scutellar	ADJ
afs-72695	74	29	tissue	tissue	NOUN
afs-72695	74	30	.	.	PUNCT
afs-72695	75	1	these	these	DET
afs-72695	75	2	protoplasts	protoplast	NOUN
afs-72695	75	3	started	start	VERB
afs-72695	75	4	cell	cell	NOUN
afs-72695	75	5	division	division	NOUN
afs-72695	75	6	3	3	NUM
afs-72695	75	7	-	-	SYM
afs-72695	75	8	5	5	NUM
afs-72695	75	9	days	day	NOUN
afs-72695	75	10	after	after	ADP
afs-72695	75	11	isolation	isolation	NOUN
afs-72695	75	12	and	and	CCONJ
afs-72695	75	13	transformation	transformation	NOUN
afs-72695	75	14	(	(	PUNCT
afs-72695	75	15	fig	fig	NOUN
afs-72695	75	16	.	.	PUNCT
afs-72695	76	1	3	3	NUM
afs-72695	76	2	)	)	PUNCT
afs-72695	77	1	and	and	CCONJ
afs-72695	77	2	continued	continue	VERB
afs-72695	77	3	further	far	ADV
afs-72695	77	4	forming	form	VERB
afs-72695	77	5	20	20	NUM
afs-72695	77	6	-	-	SYM
afs-72695	77	7	30	30	NUM
afs-72695	77	8	celled	celle	VERB
afs-72695	77	9	aggregates	aggregate	NOUN
afs-72695	77	10	.	.	PUNCT
afs-72695	78	1	this	this	DET
afs-72695	78	2	observation	observation	NOUN
afs-72695	78	3	encourages	encourage	VERB
afs-72695	78	4	us	we	PRON
afs-72695	78	5	towards	towards	ADP
afs-72695	78	6	our	our	PRON
afs-72695	78	7	further	further	ADJ
afs-72695	78	8	goals	goal	NOUN
afs-72695	78	9	to	to	PART
afs-72695	78	10	obtain	obtain	VERB
afs-72695	78	11	regenerated	regenerated	ADJ
afs-72695	78	12	transgenic	transgenic	NOUN
afs-72695	78	13	plants	plant	NOUN
afs-72695	78	14	from	from	ADP
afs-72695	78	15	these	these	DET
afs-72695	78	16	transformed	transform	VERB
afs-72695	78	17	protoplasts	protoplast	NOUN
afs-72695	78	18	.	.	PUNCT
afs-72695	79	1	it	it	PRON
afs-72695	79	2	is	be	AUX
afs-72695	79	3	well	well	ADV
afs-72695	79	4	known	know	VERB
afs-72695	79	5	from	from	ADP
afs-72695	79	6	the	the	DET
afs-72695	79	7	earlier	early	ADJ
afs-72695	79	8	studies	study	NOUN
afs-72695	79	9	that	that	SCONJ
afs-72695	79	10	the	the	DET
afs-72695	79	11	scutellar	scutellar	ADJ
afs-72695	79	12	tissue	tissue	NOUN
afs-72695	79	13	in	in	ADP
afs-72695	79	14	monocot	monocot	ADJ
afs-72695	79	15	species	specie	NOUN
afs-72695	79	16	has	have	AUX
afs-72695	79	17	a	a	DET
afs-72695	79	18	great	great	ADJ
afs-72695	79	19	regeneration	regeneration	NOUN
afs-72695	79	20	capacity	capacity	NOUN
afs-72695	79	21	(	(	PUNCT
afs-72695	79	22	fujimura	fujimura	NOUN
afs-72695	79	23	et	et	PROPN
afs-72695	79	24	al	al	PROPN
afs-72695	79	25	.	.	PROPN
afs-72695	79	26	1985	1985	NUM
afs-72695	79	27	)	)	PUNCT
afs-72695	79	28	.	.	PUNCT
afs-72695	80	1	protoplast	protoplast	NOUN
afs-72695	80	2	transformation	transformation	NOUN
afs-72695	80	3	and	and	CCONJ
afs-72695	80	4	transient	transient	ADJ
afs-72695	80	5	gene	gene	NOUN
afs-72695	80	6	expression	expression	NOUN
afs-72695	80	7	protoplasts	protoplast	NOUN
afs-72695	80	8	isolated	isolate	VERB
afs-72695	80	9	directly	directly	ADV
afs-72695	80	10	from	from	ADP
afs-72695	80	11	scutellum	scutellum	NOUN
afs-72695	80	12	-	-	PUNCT
afs-72695	80	13	embryo	embryo	NOUN
afs-72695	80	14	complexes	complex	NOUN
afs-72695	80	15	were	be	AUX
afs-72695	80	16	succesfully	succesfully	ADV
afs-72695	80	17	transformed	transform	VERB
afs-72695	80	18	with	with	ADP
afs-72695	80	19	the	the	DET
afs-72695	80	20	peg	peg	NOUN
afs-72695	80	21	method	method	NOUN
afs-72695	80	22	showing	show	VERB
afs-72695	80	23	transient	transient	ADJ
afs-72695	80	24	expression	expression	NOUN
afs-72695	80	25	of	of	ADP
afs-72695	80	26	the	the	DET
afs-72695	80	27	reporter	reporter	NOUN
afs-72695	80	28	gene	gene	NOUN
afs-72695	80	29	under	under	ADP
afs-72695	80	30	the	the	DET
afs-72695	80	31	control	control	NOUN
afs-72695	80	32	of	of	ADP
afs-72695	80	33	the	the	DET
afs-72695	80	34	rice	rice	NOUN
afs-72695	80	35	actinl	actinl	NOUN
afs-72695	80	36	promoter	promoter	NOUN
afs-72695	80	37	,	,	PUNCT
afs-72695	80	38	similarly	similarly	ADV
afs-72695	80	39	to	to	ADP
afs-72695	80	40	the	the	DET
afs-72695	80	41	results	result	NOUN
afs-72695	80	42	in	in	ADP
afs-72695	80	43	rice	rice	NOUN
afs-72695	80	44	transformation	transformation	NOUN
afs-72695	80	45	experiments	experiment	NOUN
afs-72695	80	46	(	(	PUNCT
afs-72695	80	47	mcelroy	mcelroy	PROPN
afs-72695	80	48	et	et	PROPN
afs-72695	80	49	al	al	PROPN
afs-72695	80	50	.	.	PUNCT
afs-72695	81	1	1991).this	1991).this	NUM
afs-72695	81	2	became	become	VERB
afs-72695	81	3	evident	evident	ADJ
afs-72695	81	4	after	after	ADP
afs-72695	81	5	treating	treat	VERB
afs-72695	81	6	the	the	DET
afs-72695	81	7	transformed	transform	VERB
afs-72695	81	8	cells	cell	NOUN
afs-72695	81	9	with	with	ADP
afs-72695	81	10	x	x	NOUN
afs-72695	81	11	-	-	NOUN
afs-72695	81	12	gluc	gluc	NOUN
afs-72695	81	13	.	.	PUNCT
afs-72695	82	1	some	some	PRON
afs-72695	82	2	of	of	ADP
afs-72695	82	3	the	the	DET
afs-72695	82	4	cells	cell	NOUN
afs-72695	82	5	showed	show	VERB
afs-72695	82	6	the	the	DET
afs-72695	82	7	typical	typical	ADJ
afs-72695	82	8	blue	blue	ADJ
afs-72695	82	9	colour	colour	NOUN
afs-72695	82	10	resulting	result	VERB
afs-72695	82	11	from	from	ADP
afs-72695	82	12	the	the	DET
afs-72695	82	13	reaction	reaction	NOUN
afs-72695	82	14	between	between	ADP
afs-72695	82	15	the	the	DET
afs-72695	82	16	enzyme	enzyme	NOUN
afs-72695	82	17	and	and	CCONJ
afs-72695	82	18	its	its	PRON
afs-72695	82	19	substrate	substrate	NOUN
afs-72695	82	20	(	(	PUNCT
afs-72695	82	21	fig	fig	NOUN
afs-72695	82	22	4	4	NUM
afs-72695	82	23	.	.	PUNCT
afs-72695	82	24	)	)	PUNCT
afs-72695	82	25	.	.	PUNCT
afs-72695	83	1	the	the	DET
afs-72695	83	2	frequency	frequency	NOUN
afs-72695	83	3	of	of	ADP
afs-72695	83	4	transformed	transform	VERB
afs-72695	83	5	cells	cell	NOUN
afs-72695	83	6	was	be	AUX
afs-72695	83	7	estimated	estimate	VERB
afs-72695	83	8	by	by	ADP
afs-72695	83	9	visual	visual	ADJ
afs-72695	83	10	examination	examination	NOUN
afs-72695	83	11	.	.	PUNCT
afs-72695	84	1	the	the	DET
afs-72695	84	2	frequency	frequency	NOUN
afs-72695	84	3	of	of	ADP
afs-72695	84	4	the	the	DET
afs-72695	84	5	transformation	transformation	NOUN
afs-72695	84	6	events	event	NOUN
afs-72695	84	7	that	that	PRON
afs-72695	84	8	showed	show	VERB
afs-72695	84	9	transient	transient	ADJ
afs-72695	84	10	expression	expression	NOUN
afs-72695	84	11	of	of	ADP
afs-72695	84	12	the	the	DET
afs-72695	84	13	gus	gus	NOUN
afs-72695	84	14	gene	gene	NOUN
afs-72695	84	15	was	be	AUX
afs-72695	84	16	estimated	estimate	VERB
afs-72695	84	17	to	to	PART
afs-72695	84	18	be	be	AUX
afs-72695	84	19	in	in	ADP
afs-72695	84	20	some	some	DET
afs-72695	84	21	cases	case	NOUN
afs-72695	84	22	up	up	ADP
afs-72695	84	23	to	to	PART
afs-72695	84	24	6	6	NUM
afs-72695	84	25	%	%	NOUN
afs-72695	84	26	of	of	ADP
afs-72695	84	27	the	the	DET
afs-72695	84	28	isolated	isolate	VERB
afs-72695	84	29	and	and	CCONJ
afs-72695	84	30	transformed	transform	VERB
afs-72695	84	31	protoplasts	protoplast	NOUN
afs-72695	84	32	.	.	PUNCT
afs-72695	85	1	in	in	ADP
afs-72695	85	2	plant	plant	NOUN
afs-72695	85	3	molecular	molecular	ADJ
afs-72695	85	4	biology	biology	NOUN
afs-72695	85	5	research	research	NOUN
afs-72695	85	6	cell	cell	NOUN
afs-72695	85	7	suspension	suspension	NOUN
afs-72695	85	8	is	be	AUX
afs-72695	85	9	the	the	DET
afs-72695	85	10	most	most	ADV
afs-72695	85	11	common	common	ADJ
afs-72695	85	12	source	source	NOUN
afs-72695	85	13	of	of	ADP
afs-72695	85	14	barley	barley	NOUN
afs-72695	85	15	protoplasts	protoplast	NOUN
afs-72695	85	16	used	use	VERB
afs-72695	85	17	in	in	ADP
afs-72695	85	18	the	the	DET
afs-72695	85	19	investigation	investigation	NOUN
afs-72695	85	20	of	of	ADP
afs-72695	85	21	new	new	ADJ
afs-72695	85	22	gene	gene	NOUN
afs-72695	85	23	constructs	construct	VERB
afs-72695	85	24	.	.	PUNCT
afs-72695	86	1	the	the	DET
afs-72695	86	2	establishment	establishment	NOUN
afs-72695	86	3	of	of	ADP
afs-72695	86	4	a	a	DET
afs-72695	86	5	suspensionprotoplast	suspensionprotoplast	NOUN
afs-72695	86	6	system	system	NOUN
afs-72695	86	7	in	in	ADP
afs-72695	86	8	monocots	monocot	NOUN
afs-72695	86	9	is	be	AUX
afs-72695	86	10	a	a	DET
afs-72695	86	11	time	time	NOUN
afs-72695	86	12	consuming	consume	VERB
afs-72695	86	13	process	process	NOUN
afs-72695	86	14	(	(	PUNCT
afs-72695	86	15	jähne	jähne	NOUN
afs-72695	86	16	et	et	PROPN
afs-72695	86	17	al	al	PROPN
afs-72695	86	18	.	.	PROPN
afs-72695	86	19	1991	1991	NUM
afs-72695	86	20	)	)	PUNCT
afs-72695	86	21	taking	take	VERB
afs-72695	86	22	three	three	NUM
afs-72695	86	23	to	to	PART
afs-72695	86	24	six	six	NUM
afs-72695	86	25	months	month	NOUN
afs-72695	86	26	of	of	ADP
afs-72695	86	27	culture	culture	NOUN
afs-72695	86	28	until	until	SCONJ
afs-72695	86	29	the	the	DET
afs-72695	86	30	first	first	ADJ
afs-72695	86	31	protoplasts	protoplast	NOUN
afs-72695	86	32	can	can	AUX
afs-72695	86	33	be	be	AUX
afs-72695	86	34	digested	digest	VERB
afs-72695	86	35	and	and	CCONJ
afs-72695	86	36	transformed	transform	VERB
afs-72695	86	37	(	(	PUNCT
afs-72695	86	38	junker	junker	PROPN
afs-72695	86	39	et	et	PROPN
afs-72695	86	40	al	al	PROPN
afs-72695	86	41	.	.	PROPN
afs-72695	86	42	1987	1987	NUM
afs-72695	86	43	)	)	PUNCT
afs-72695	86	44	.	.	PUNCT
afs-72695	87	1	to	to	PART
afs-72695	87	2	reduce	reduce	VERB
afs-72695	87	3	this	this	DET
afs-72695	87	4	time	time	NOUN
afs-72695	87	5	,	,	PUNCT
afs-72695	87	6	our	our	PRON
afs-72695	87	7	aim	aim	NOUN
afs-72695	87	8	was	be	AUX
afs-72695	87	9	to	to	PART
afs-72695	87	10	set	set	VERB
afs-72695	87	11	up	up	ADP
afs-72695	87	12	a	a	DET
afs-72695	87	13	rapid	rapid	ADJ
afs-72695	87	14	system	system	NOUN
afs-72695	87	15	for	for	ADP
afs-72695	87	16	producing	produce	VERB
afs-72695	87	17	viable	viable	ADJ
afs-72695	87	18	protoplasts	protoplast	NOUN
afs-72695	87	19	suitable	suitable	ADJ
afs-72695	87	20	for	for	ADP
afs-72695	87	21	transformation	transformation	NOUN
afs-72695	87	22	and	and	CCONJ
afs-72695	87	23	transient	transient	ADJ
afs-72695	87	24	gene	gene	NOUN
afs-72695	87	25	expression	expression	NOUN
afs-72695	87	26	studies	study	NOUN
afs-72695	87	27	.	.	PUNCT
afs-72695	88	1	the	the	DET
afs-72695	88	2	embryo	embryo	NOUN
afs-72695	88	3	-	-	PUNCT
afs-72695	88	4	scutellum	scutellum	NOUN
afs-72695	88	5	derived	derive	VERB
afs-72695	88	6	protoplast	protoplast	NOUN
afs-72695	88	7	system	system	NOUN
afs-72695	88	8	seemed	seem	VERB
afs-72695	88	9	to	to	PART
afs-72695	88	10	fullfil	fullfil	VERB
afs-72695	88	11	these	these	DET
afs-72695	88	12	requirements	requirement	NOUN
afs-72695	88	13	.	.	PUNCT
afs-72695	89	1	only	only	ADV
afs-72695	89	2	24	24	NUM
afs-72695	89	3	hours	hour	NOUN
afs-72695	89	4	was	be	AUX
afs-72695	89	5	needed	need	VERB
afs-72695	89	6	prior	prior	ADV
afs-72695	89	7	the	the	DET
afs-72695	89	8	protoplast	protoplast	NOUN
afs-72695	89	9	isolation	isolation	NOUN
afs-72695	89	10	and	and	CCONJ
afs-72695	89	11	transformation	transformation	NOUN
afs-72695	89	12	instead	instead	ADV
afs-72695	89	13	of	of	ADP
afs-72695	89	14	several	several	ADJ
afs-72695	89	15	months	month	NOUN
afs-72695	89	16	of	of	ADP
afs-72695	89	17	subculturing	subculturing	NOUN
afs-72695	89	18	.	.	PUNCT
afs-72695	90	1	this	this	DET
afs-72695	90	2	system	system	NOUN
afs-72695	90	3	was	be	AUX
afs-72695	90	4	also	also	ADV
afs-72695	90	5	suitable	suitable	ADJ
afs-72695	90	6	for	for	ADP
afs-72695	90	7	obtaining	obtain	VERB
afs-72695	90	8	4.5	4.5	NUM
afs-72695	90	9	%	%	NOUN
afs-72695	90	10	of	of	ADP
afs-72695	90	11	the	the	DET
afs-72695	90	12	transformed	transform	VERB
afs-72695	90	13	cells	cell	NOUN
afs-72695	90	14	showing	show	VERB
afs-72695	90	15	transient	transient	ADJ
afs-72695	90	16	gene	gene	NOUN
afs-72695	90	17	expression	expression	NOUN
afs-72695	90	18	.	.	PUNCT
afs-72695	91	1	the	the	DET
afs-72695	91	2	time	time	NOUN
afs-72695	91	3	needs	need	NOUN
afs-72695	91	4	of	of	ADP
afs-72695	91	5	the	the	DET
afs-72695	91	6	protoplast	protoplast	NOUN
afs-72695	91	7	isolation	isolation	NOUN
afs-72695	91	8	and	and	CCONJ
afs-72695	91	9	transformation	transformation	NOUN
afs-72695	91	10	system	system	NOUN
afs-72695	91	11	presented	present	VERB
afs-72695	91	12	in	in	ADP
afs-72695	91	13	this	this	DET
afs-72695	91	14	paper	paper	NOUN
afs-72695	91	15	is	be	AUX
afs-72695	91	16	comparable	comparable	ADJ
afs-72695	91	17	to	to	ADP
afs-72695	91	18	the	the	DET
afs-72695	91	19	transformation	transformation	NOUN
afs-72695	91	20	with	with	ADP
afs-72695	91	21	particle	particle	NOUN
afs-72695	91	22	bombardment	bombardment	NOUN
afs-72695	91	23	but	but	CCONJ
afs-72695	91	24	it	it	PRON
afs-72695	91	25	still	still	ADV
afs-72695	91	26	has	have	VERB
afs-72695	91	27	all	all	DET
afs-72695	91	28	the	the	DET
afs-72695	91	29	advantages	advantage	NOUN
afs-72695	91	30	of	of	ADP
afs-72695	91	31	the	the	DET
afs-72695	91	32	protoplast	protoplast	NOUN
afs-72695	91	33	system	system	NOUN
afs-72695	91	34	.	.	PUNCT
afs-72695	92	1	for	for	ADP
afs-72695	92	2	example	example	NOUN
afs-72695	92	3	,	,	PUNCT
afs-72695	92	4	as	as	SCONJ
afs-72695	92	5	protoplast	protoplast	NOUN
afs-72695	92	6	derived	derive	VERB
afs-72695	92	7	regenerants	regenerant	NOUN
afs-72695	92	8	have	have	VERB
afs-72695	92	9	a	a	DET
afs-72695	92	10	single	single	ADJ
afs-72695	92	11	cell	cell	NOUN
afs-72695	92	12	origin	origin	NOUN
afs-72695	92	13	,	,	PUNCT
afs-72695	92	14	one	one	PRON
afs-72695	92	15	can	can	AUX
afs-72695	92	16	expect	expect	VERB
afs-72695	92	17	genetic	genetic	ADJ
afs-72695	92	18	uniformity	uniformity	NOUN
afs-72695	92	19	in	in	ADP
afs-72695	92	20	the	the	DET
afs-72695	92	21	whole	whole	ADJ
afs-72695	92	22	plant	plant	NOUN
afs-72695	92	23	after	after	ADP
afs-72695	92	24	regeneration	regeneration	NOUN
afs-72695	92	25	from	from	ADP
afs-72695	92	26	transformed	transform	VERB
afs-72695	92	27	protoplast	protoplast	NOUN
afs-72695	92	28	which	which	PRON
afs-72695	92	29	is	be	AUX
afs-72695	92	30	not	not	PART
afs-72695	92	31	always	always	ADV
afs-72695	92	32	the	the	DET
afs-72695	92	33	case	case	NOUN
afs-72695	92	34	after	after	ADP
afs-72695	92	35	regeneration	regeneration	NOUN
afs-72695	92	36	from	from	ADP
afs-72695	92	37	the	the	DET
afs-72695	92	38	bombarded	bombarded	ADJ
afs-72695	92	39	material	material	NOUN
afs-72695	92	40	.	.	PUNCT
afs-72695	93	1	further	far	ADV
afs-72695	93	2	refinary	refinary	ADJ
afs-72695	93	3	of	of	ADP
afs-72695	93	4	this	this	DET
afs-72695	93	5	method	method	NOUN
afs-72695	93	6	to	to	PART
afs-72695	93	7	isolate	isolate	VERB
afs-72695	93	8	barley	barley	NOUN
afs-72695	93	9	protoplasts	protoplast	NOUN
afs-72695	93	10	directly	directly	ADV
afs-72695	93	11	from	from	ADP
afs-72695	93	12	mature	mature	ADJ
afs-72695	93	13	embryo	embryo	NOUN
afs-72695	93	14	-	-	PUNCT
afs-72695	93	15	scutellum	scutellum	NOUN
afs-72695	93	16	complexes	complex	NOUN
afs-72695	93	17	is	be	AUX
afs-72695	93	18	needed	need	VERB
afs-72695	93	19	to	to	PART
afs-72695	93	20	improve	improve	VERB
afs-72695	93	21	the	the	DET
afs-72695	93	22	yield	yield	NOUN
afs-72695	93	23	of	of	ADP
afs-72695	93	24	protoplasts	protoplast	NOUN
afs-72695	93	25	and	and	CCONJ
afs-72695	93	26	the	the	DET
afs-72695	93	27	induction	induction	NOUN
afs-72695	93	28	level	level	NOUN
afs-72695	93	29	of	of	ADP
afs-72695	93	30	cell	cell	NOUN
afs-72695	93	31	divisions	division	NOUN
afs-72695	93	32	.	.	PUNCT
afs-72695	94	1	a	a	DET
afs-72695	94	2	working	work	VERB
afs-72695	94	3	and	and	CCONJ
afs-72695	94	4	repeatable	repeatable	ADJ
afs-72695	94	5	protoplast	protoplast	NOUN
afs-72695	94	6	-	-	PUNCT
afs-72695	94	7	plant	plant	NOUN
afs-72695	94	8	regeneration	regeneration	NOUN
afs-72695	94	9	system	system	NOUN
afs-72695	94	10	based	base	VERB
afs-72695	94	11	on	on	ADP
afs-72695	94	12	a	a	DET
afs-72695	94	13	quick	quick	ADJ
afs-72695	94	14	protoplast	protoplast	NOUN
afs-72695	94	15	isolation	isolation	NOUN
afs-72695	94	16	could	could	AUX
afs-72695	94	17	pro202	pro202	VERB
afs-72695	94	18	research	research	NOUN
afs-72695	94	19	note	note	NOUN
afs-72695	94	20	research	research	NOUN
afs-72695	94	21	note	note	NOUN
afs-72695	94	22	vide	vide	NOUN
afs-72695	94	23	with	with	ADP
afs-72695	94	24	the	the	DET
afs-72695	94	25	ability	ability	NOUN
afs-72695	94	26	to	to	PART
afs-72695	94	27	produce	produce	VERB
afs-72695	94	28	fertile	fertile	ADJ
afs-72695	94	29	transgenic	transgenic	NOUN
afs-72695	94	30	plants	plant	NOUN
afs-72695	94	31	of	of	ADP
afs-72695	94	32	barley	barley	NOUN
afs-72695	94	33	within	within	ADP
afs-72695	94	34	much	much	ADV
afs-72695	94	35	shorter	short	ADJ
afs-72695	94	36	time	time	NOUN
afs-72695	94	37	than	than	ADP
afs-72695	94	38	through	through	ADP
afs-72695	94	39	the	the	DET
afs-72695	94	40	suspension	suspension	NOUN
afs-72695	94	41	-	-	PUNCT
afs-72695	94	42	protoplast	protoplast	NOUN
afs-72695	94	43	-	-	PUNCT
afs-72695	94	44	transgenic	transgenic	NOUN
afs-72695	94	45	plant	plant	NOUN
afs-72695	94	46	system	system	NOUN
afs-72695	94	47	.	.	PUNCT
afs-72695	95	1	acknowledgements	acknowledgement	NOUN
afs-72695	95	2	.	.	PUNCT
afs-72695	96	1	the	the	DET
afs-72695	96	2	authors	author	NOUN
afs-72695	96	3	are	be	AUX
afs-72695	96	4	grateful	grateful	ADJ
afs-72695	96	5	to	to	ADP
afs-72695	96	6	the	the	DET
afs-72695	96	7	agricultural	agricultural	ADJ
afs-72695	96	8	research	research	NOUN
afs-72695	96	9	centre	centre	NOUN
afs-72695	96	10	of	of	ADP
afs-72695	96	11	finland	finland	PROPN
afs-72695	96	12	,	,	PUNCT
afs-72695	96	13	jokioinen	jokioinen	PROPN
afs-72695	96	14	,	,	PUNCT
afs-72695	96	15	finland	finland	PROPN
afs-72695	96	16	,	,	PUNCT
afs-72695	96	17	and	and	CCONJ
afs-72695	96	18	agricultural	agricultural	ADJ
afs-72695	96	19	biotechnology	biotechnology	NOUN
afs-72695	96	20	centre	centre	NOUN
afs-72695	96	21	,	,	PUNCT
afs-72695	96	22	gödöllö	gödöllö	NOUN
afs-72695	96	23	,	,	PUNCT
afs-72695	96	24	hungary	hungary	PROPN
afs-72695	96	25	,	,	PUNCT
afs-72695	96	26	and	and	CCONJ
afs-72695	96	27	to	to	ADP
afs-72695	96	28	the	the	DET
afs-72695	96	29	ministry	ministry	PROPN
afs-72695	96	30	of	of	ADP
afs-72695	96	31	agriculture	agriculture	PROPN
afs-72695	96	32	and	and	CCONJ
afs-72695	96	33	forestry	forestry	NOUN
afs-72695	96	34	of	of	ADP
afs-72695	96	35	finland	finland	PROPN
afs-72695	96	36	for	for	ADP
afs-72695	96	37	providing	provide	VERB
afs-72695	96	38	the	the	DET
afs-72695	96	39	facilities	facility	NOUN
afs-72695	96	40	and	and	CCONJ
afs-72695	96	41	finance	finance	NOUN
afs-72695	96	42	for	for	ADP
afs-72695	96	43	this	this	DET
afs-72695	96	44	study	study	NOUN
afs-72695	96	45	.	.	PUNCT
afs-72695	97	1	references	reference	NOUN
afs-72695	97	2	ahmed	ahmed	PROPN
afs-72695	97	3	,	,	PUNCT
afs-72695	97	4	z.	z.	PROPN
afs-72695	97	5	k.	k.	PROPN
afs-72695	97	6	&	&	CCONJ
afs-72695	97	7	sägi	sägi	PROPN
afs-72695	97	8	,	,	PUNCT
afs-72695	97	9	f.	f.	PROPN
afs-72695	97	10	1993	1993	NUM
afs-72695	97	11	.	.	PUNCT
afs-72695	98	1	culture	culture	NOUN
afs-72695	98	2	of	of	ADP
afs-72695	98	3	and	and	CCONJ
afs-72695	98	4	fertile	fertile	ADJ
afs-72695	98	5	plant	plant	NOUN
afs-72695	98	6	regeneration	regeneration	NOUN
afs-72695	98	7	from	from	ADP
afs-72695	98	8	regenerable	regenerable	ADJ
afs-72695	98	9	embryogenic	embryogenic	ADJ
afs-72695	98	10	suspension	suspension	NOUN
afs-72695	98	11	cell	cell	NOUN
afs-72695	98	12	-	-	PUNCT
afs-72695	98	13	derived	derive	VERB
afs-72695	98	14	protoplasts	protoplast	NOUN
afs-72695	98	15	ofwheat	ofwheat	VERB
afs-72695	98	16	(	(	PUNCT
afs-72695	98	17	triticum	triticum	NOUN
afs-72695	98	18	aestivum	aestivum	PROPN
afs-72695	98	19	l.	l.	PROPN
afs-72695	98	20	)	)	PUNCT
afs-72695	98	21	.	.	PUNCT
afs-72695	99	1	plant	plant	NOUN
afs-72695	99	2	cell	cell	NOUN
afs-72695	99	3	reports	report	VERB
afs-72695	99	4	12	12	NUM
afs-72695	99	5	:	:	PUNCT
afs-72695	99	6	175	175	NUM
afs-72695	99	7	-	-	SYM
afs-72695	99	8	179	179	NUM
afs-72695	99	9	.	.	PUNCT
afs-72695	100	1	chang	chang	PROPN
afs-72695	100	2	,	,	PUNCT
afs-72695	100	3	y.-f	y.-f	PROPN
afs-72695	100	4	.	.	PUNCT
afs-72695	100	5	,	,	PUNCT
afs-72695	100	6	wang	wang	PROPN
afs-72695	100	7	,	,	PUNCT
afs-72695	100	8	w.ch	w.ch	PROPN
afs-72695	100	9	.	.	PROPN
afs-72695	100	10	,	,	PUNCT
afs-72695	100	11	warfield	warfield	PROPN
afs-72695	100	12	,	,	PUNCT
afs-72695	100	13	c.y	c.y	PROPN
afs-72695	100	14	.	.	PROPN
afs-72695	100	15	,	,	PUNCT
afs-72695	100	16	nguyen	nguyen	PROPN
afs-72695	100	17	,	,	PUNCT
afs-72695	100	18	h.t	h.t	PROPN
afs-72695	100	19	.	.	PROPN
afs-72695	100	20	&	&	CCONJ
afs-72695	100	21	wong	wong	PROPN
afs-72695	100	22	,	,	PUNCT
afs-72695	100	23	j.r	j.r	PROPN
afs-72695	100	24	.	.	PROPN
afs-72695	100	25	1991	1991	NUM
afs-72695	100	26	.	.	PUNCT
afs-72695	101	1	plant	plant	NOUN
afs-72695	101	2	regeneration	regeneration	NOUN
afs-72695	101	3	from	from	ADP
afs-72695	101	4	protoplasts	protoplast	NOUN
afs-72695	101	5	isolated	isolate	VERB
afs-72695	101	6	from	from	ADP
afs-72695	101	7	long	long	ADJ
afs-72695	101	8	-	-	PUNCT
afs-72695	101	9	term	term	NOUN
afs-72695	101	10	cultures	culture	NOUN
afs-72695	101	11	of	of	ADP
afs-72695	101	12	wheat	wheat	NOUN
afs-72695	101	13	(	(	PUNCT
afs-72695	101	14	triticum	triticum	NOUN
afs-72695	101	15	aestivum	aestivum	PROPN
afs-72695	101	16	l.	l.	PROPN
afs-72695	101	17	)	)	PUNCT
afs-72695	101	18	.	.	PUNCT
afs-72695	102	1	plant	plant	NOUN
afs-72695	102	2	cell	cell	NOUN
afs-72695	102	3	reports	report	VERB
afs-72695	102	4	9	9	NUM
afs-72695	102	5	:	:	SYM
afs-72695	102	6	611	611	NUM
afs-72695	102	7	-	-	SYM
afs-72695	102	8	614	614	NUM
afs-72695	102	9	.	.	PUNCT
afs-72695	103	1	fig	fig	NOUN
afs-72695	103	2	.	.	PUNCT
afs-72695	104	1	1	1	X
afs-72695	104	2	.	.	NUM
afs-72695	104	3	excised	excise	VERB
afs-72695	104	4	mature	mature	ADJ
afs-72695	104	5	barley	barley	NOUN
afs-72695	104	6	scutellum	scutellum	NOUN
afs-72695	104	7	-	-	PUNCT
afs-72695	104	8	embryo	embryo	NOUN
afs-72695	104	9	complex	complex	NOUN
afs-72695	104	10	after	after	ADP
afs-72695	104	11	24	24	NUM
afs-72695	104	12	hours	hour	NOUN
afs-72695	104	13	of	of	ADP
afs-72695	104	14	imbibition	imbibition	NOUN
afs-72695	104	15	in	in	ADP
afs-72695	104	16	distilled	distil	VERB
afs-72695	104	17	,	,	PUNCT
afs-72695	104	18	sterilized	sterilize	VERB
afs-72695	104	19	water	water	NOUN
afs-72695	104	20	.	.	PUNCT
afs-72695	105	1	scale	scale	NOUN
afs-72695	105	2	bar	bar	NOUN
afs-72695	106	1	=	=	PUNCT
afs-72695	107	1	i	i	PRON
afs-72695	107	2	mm	mm	INTJ
afs-72695	107	3	.	.	PUNCT
afs-72695	108	1	(	(	PUNCT
afs-72695	108	2	photo	photo	NOUN
afs-72695	108	3	:	:	PUNCT
afs-72695	108	4	matti	matti	PROPN
afs-72695	108	5	puolimatka	puolimatka	PROPN
afs-72695	108	6	)	)	PUNCT
afs-72695	108	7	fig	fig	NOUN
afs-72695	108	8	.	.	PUNCT
afs-72695	109	1	2	2	X
afs-72695	109	2	.	.	X
afs-72695	109	3	barley	barley	NOUN
afs-72695	109	4	protoplasts	protoplast	NOUN
afs-72695	109	5	isolated	isolate	VERB
afs-72695	109	6	from	from	ADP
afs-72695	109	7	the	the	DET
afs-72695	109	8	mature	mature	ADJ
afs-72695	109	9	scutellum	scutellum	NOUN
afs-72695	109	10	-	-	PUNCT
afs-72695	109	11	embryo	embryo	NOUN
afs-72695	109	12	complexes	complex	NOUN
afs-72695	109	13	of	of	ADP
afs-72695	109	14	’	'	PUNCT
afs-72695	109	15	kymppi	kymppi	PROPN
afs-72695	109	16	’	'	PUNCT
afs-72695	109	17	.	.	PUNCT
afs-72695	110	1	(	(	PUNCT
afs-72695	110	2	photo	photo	NOUN
afs-72695	110	3	:	:	PUNCT
afs-72695	110	4	barnabäs	barnabäs	NOUN
afs-72695	110	5	jenes	jene	NOUN
afs-72695	110	6	)	)	PUNCT
afs-72695	110	7	fig	fig	NOUN
afs-72695	110	8	.	.	PUNCT
afs-72695	111	1	3	3	X
afs-72695	111	2	.	.	X
afs-72695	111	3	cell	cell	NOUN
afs-72695	111	4	divisions	division	NOUN
afs-72695	111	5	in	in	ADP
afs-72695	111	6	barley	barley	NOUN
afs-72695	111	7	protoplasts	protoplast	NOUN
afs-72695	111	8	.	.	PUNCT
afs-72695	112	1	(	(	PUNCT
afs-72695	112	2	photo	photo	NOUN
afs-72695	112	3	;	;	PUNCT
afs-72695	112	4	bama	bama	NOUN
afs-72695	112	5	bäs	bäs	NOUN
afs-72695	112	6	jenes	jene	VERB
afs-72695	112	7	)	)	PUNCT
afs-72695	112	8	fig	fig	NOUN
afs-72695	112	9	.	.	PUNCT
afs-72695	113	1	4	4	X
afs-72695	113	2	.	.	X
afs-72695	113	3	blue	blue	ADJ
afs-72695	113	4	colour	colour	NOUN
afs-72695	113	5	shows	show	VERB
afs-72695	113	6	transient	transient	VERB
afs-72695	113	7	gus	gus	NOUN
afs-72695	113	8	gene	gene	NOUN
afs-72695	113	9	activity	activity	NOUN
afs-72695	113	10	in	in	ADP
afs-72695	113	11	the	the	DET
afs-72695	113	12	transformed	transform	VERB
afs-72695	113	13	protoplasts	protoplast	NOUN
afs-72695	113	14	of	of	ADP
afs-72695	113	15	barley	barley	NOUN
afs-72695	113	16	.	.	PUNCT
afs-72695	114	1	(	(	PUNCT
afs-72695	114	2	photo	photo	NOUN
afs-72695	114	3	:	:	PUNCT
afs-72695	114	4	barnabäs	barnabäs	NOUN
afs-72695	114	5	jenes	jene	NOUN
afs-72695	114	6	)	)	PUNCT
afs-72695	114	7	203	203	NUM
afs-72695	114	8	agricultural	agricultural	ADJ
afs-72695	114	9	science	science	NOUN
afs-72695	114	10	in	in	ADP
afs-72695	114	11	finland	finland	PROPN
afs-72695	114	12	3	3	NUM
afs-72695	114	13	(	(	PUNCT
afs-72695	114	14	1994	1994	NUM
afs-72695	114	15	)	)	PUNCT
afs-72695	114	16	research	research	NOUN
afs-72695	114	17	note	note	NOUN
afs-72695	114	18	diaz	diaz	PROPN
afs-72695	114	19	,	,	PUNCT
afs-72695	114	20	i.	i.	PROPN
afs-72695	114	21	&	&	CCONJ
afs-72695	114	22	carbonero	carbonero	PROPN
afs-72695	114	23	,	,	PUNCT
afs-72695	114	24	p.	p.	NOUN
afs-72695	114	25	1992	1992	NUM
afs-72695	114	26	.	.	PUNCT
afs-72695	115	1	isolation	isolation	NOUN
afs-72695	115	2	of	of	ADP
afs-72695	115	3	protoplasts	protoplast	NOUN
afs-72695	115	4	from	from	ADP
afs-72695	115	5	developing	develop	VERB
afs-72695	115	6	barley	barley	NOUN
afs-72695	115	7	endosperm	endosperm	NOUN
afs-72695	115	8	:	:	PUNCT
afs-72695	115	9	a	a	DET
afs-72695	115	10	tool	tool	NOUN
afs-72695	115	11	for	for	ADP
afs-72695	115	12	transient	transient	ADJ
afs-72695	115	13	expression	expression	NOUN
afs-72695	115	14	studies	study	NOUN
afs-72695	115	15	.	.	PUNCT
afs-72695	116	1	plant	plant	NOUN
afs-72695	116	2	cell	cell	NOUN
afs-72695	116	3	reports	report	VERB
afs-72695	116	4	10	10	NUM
afs-72695	116	5	:	:	SYM
afs-72695	116	6	595	595	NUM
afs-72695	116	7	-	-	SYM
afs-72695	116	8	598	598	NUM
afs-72695	116	9	.	.	PUNCT
afs-72695	117	1	fujimura	fujimura	NOUN
afs-72695	117	2	,	,	PUNCT
afs-72695	117	3	t.	t.	PROPN
afs-72695	117	4	,	,	PUNCT
afs-72695	117	5	sakurai	sakurai	PROPN
afs-72695	117	6	,	,	PUNCT
afs-72695	117	7	m.	m.	NOUN
afs-72695	117	8	,	,	PUNCT
afs-72695	117	9	akagi	akagi	PROPN
afs-72695	117	10	,	,	PUNCT
afs-72695	117	11	h.	h.	PROPN
afs-72695	117	12	,	,	PUNCT
afs-72695	117	13	negishi	negishi	PROPN
afs-72695	117	14	,	,	PUNCT
afs-72695	117	15	t.	t.	PROPN
afs-72695	117	16	&	&	CCONJ
afs-72695	117	17	hirose	hirose	PROPN
afs-72695	117	18	,	,	PUNCT
afs-72695	117	19	a.	a.	NOUN
afs-72695	117	20	1985	1985	NUM
afs-72695	117	21	.	.	PUNCT
afs-72695	118	1	regeneration	regeneration	NOUN
afs-72695	118	2	of	of	ADP
afs-72695	118	3	rice	rice	NOUN
afs-72695	118	4	plants	plant	NOUN
afs-72695	118	5	from	from	ADP
afs-72695	118	6	protoplasts	protoplast	NOUN
afs-72695	118	7	.	.	PUNCT
afs-72695	119	1	plant	plant	NOUN
afs-72695	119	2	tissue	tissue	NOUN
afs-72695	119	3	culture	culture	NOUN
afs-72695	119	4	letters	letter	NOUN
afs-72695	119	5	2	2	NUM
afs-72695	119	6	:	:	SYM
afs-72695	119	7	74	74	NUM
afs-72695	119	8	-	-	SYM
afs-72695	119	9	75	75	NUM
afs-72695	119	10	.	.	PUNCT
afs-72695	120	1	jähne	jähne	PROPN
afs-72695	120	2	,	,	PUNCT
afs-72695	120	3	a.	a.	NOUN
afs-72695	120	4	,	,	PUNCT
afs-72695	120	5	lazzeri	lazzeri	PROPN
afs-72695	120	6	,	,	PUNCT
afs-72695	120	7	p.	p.	NOUN
afs-72695	120	8	a.	a.	PROPN
afs-72695	120	9	&	&	CCONJ
afs-72695	120	10	lörz	lörz	PROPN
afs-72695	120	11	,	,	PUNCT
afs-72695	120	12	h.	h.	PROPN
afs-72695	120	13	1991	1991	NUM
afs-72695	120	14	.	.	PUNCT
afs-72695	121	1	regeneration	regeneration	NOUN
afs-72695	121	2	of	of	ADP
afs-72695	121	3	fertile	fertile	ADJ
afs-72695	121	4	plants	plant	NOUN
afs-72695	121	5	from	from	ADP
afs-72695	121	6	protoplasts	protoplast	NOUN
afs-72695	121	7	derived	derive	VERB
afs-72695	121	8	from	from	ADP
afs-72695	121	9	embryogenic	embryogenic	ADJ
afs-72695	121	10	cell	cell	NOUN
afs-72695	121	11	suspension	suspension	NOUN
afs-72695	121	12	of	of	ADP
afs-72695	121	13	barley	barley	NOUN
afs-72695	121	14	(	(	PUNCT
afs-72695	121	15	hordeum	hordeum	PROPN
afs-72695	121	16	vulgare	vulgare	PROPN
afs-72695	121	17	l.	l.	PROPN
afs-72695	121	18	)	)	PUNCT
afs-72695	121	19	.	.	PUNCT
afs-72695	122	1	plant	plant	NOUN
afs-72695	122	2	cell	cell	NOUN
afs-72695	122	3	reports	report	VERB
afs-72695	122	4	10	10	NUM
afs-72695	122	5	:	:	SYM
afs-72695	122	6	1	1	NUM
afs-72695	122	7	-	-	SYM
afs-72695	122	8	6	6	NUM
afs-72695	122	9	.	.	PUNCT
afs-72695	123	1	jefferson	jefferson	PROPN
afs-72695	123	2	,	,	PUNCT
afs-72695	123	3	r.	r.	PROPN
afs-72695	123	4	a.	a.	NOUN
afs-72695	123	5	1987.assaying	1987.assaye	VERB
afs-72695	123	6	chimeric	chimeric	ADJ
afs-72695	123	7	genes	gene	NOUN
afs-72695	123	8	in	in	ADP
afs-72695	123	9	plants	plant	NOUN
afs-72695	123	10	:	:	PUNCT
afs-72695	123	11	the	the	DET
afs-72695	123	12	gus	gus	NOUN
afs-72695	123	13	gene	gene	NOUN
afs-72695	123	14	fusion	fusion	NOUN
afs-72695	123	15	system	system	NOUN
afs-72695	123	16	.	.	PUNCT
afs-72695	124	1	plant	plant	NOUN
afs-72695	124	2	molecular	molecular	ADJ
afs-72695	124	3	and	and	CCONJ
afs-72695	124	4	biological	biological	ADJ
afs-72695	124	5	reports	report	NOUN
afs-72695	124	6	5	5	NUM
afs-72695	124	7	:	:	PUNCT
afs-72695	124	8	387	387	NUM
afs-72695	124	9	-	-	SYM
afs-72695	124	10	405	405	NUM
afs-72695	124	11	.	.	PUNCT
afs-72695	125	1	-	-	PUNCT
afs-72695	125	2	,	,	PUNCT
afs-72695	125	3	burgess	burgess	NOUN
afs-72695	125	4	,	,	PUNCT
afs-72695	125	5	s.	s.	PROPN
afs-72695	125	6	m.	m.	PROPN
afs-72695	125	7	&	&	CCONJ
afs-72695	125	8	hirsh	hirsh	PROPN
afs-72695	125	9	,	,	PUNCT
afs-72695	125	10	d.	d.	PROPN
afs-72695	125	11	1986	1986	NUM
afs-72695	125	12	.	.	PUNCT
afs-72695	126	1	p	p	X
afs-72695	126	2	-	-	PUNCT
afs-72695	126	3	glucuronidase	glucuronidase	NOUN
afs-72695	126	4	from	from	ADP
afs-72695	126	5	escherichia	escherichia	NOUN
afs-72695	126	6	coli	coli	NOUN
afs-72695	126	7	as	as	ADP
afs-72695	126	8	a	a	DET
afs-72695	126	9	gene	gene	NOUN
afs-72695	126	10	fusion	fusion	NOUN
afs-72695	126	11	marker	marker	NOUN
afs-72695	126	12	.	.	PUNCT
afs-72695	127	1	proceedings	proceeding	NOUN
afs-72695	127	2	of	of	ADP
afs-72695	127	3	the	the	DET
afs-72695	127	4	national	national	PROPN
afs-72695	127	5	academy	academy	PROPN
afs-72695	127	6	of	of	ADP
afs-72695	127	7	sciences	sciences	PROPN
afs-72695	127	8	.	.	PUNCT
afs-72695	128	1	usa	usa	PROPN
afs-72695	128	2	.	.	PROPN
afs-72695	129	1	83:8447	83:8447	NUM
afs-72695	129	2	-	-	PUNCT
afs-72695	129	3	8451	8451	NUM
afs-72695	129	4	.	.	PUNCT
afs-72695	130	1	jenes	jene	NOUN
afs-72695	130	2	,	,	PUNCT
afs-72695	130	3	8	8	NUM
afs-72695	130	4	.	.	NUM
afs-72695	130	5	,	,	PUNCT
afs-72695	130	6	moore	moore	PROPN
afs-72695	130	7	,	,	PUNCT
afs-72695	130	8	h.	h.	PROPN
afs-72695	130	9	,	,	PUNCT
afs-72695	130	10	zhang	zhang	PROPN
afs-72695	130	11	,	,	PUNCT
afs-72695	130	12	w.	w.	PROPN
afs-72695	130	13	,	,	PUNCT
afs-72695	130	14	cao	cao	PROPN
afs-72695	130	15	,	,	PUNCT
afs-72695	130	16	j.	j.	PROPN
afs-72695	130	17	&	&	CCONJ
afs-72695	130	18	wu	wu	PROPN
afs-72695	130	19	,	,	PUNCT
afs-72695	130	20	r.	r.	PROPN
afs-72695	130	21	1992	1992	NUM
afs-72695	130	22	.	.	PUNCT
afs-72695	131	1	techniques	technique	NOUN
afs-72695	131	2	for	for	ADP
afs-72695	131	3	gene	gene	NOUN
afs-72695	131	4	transfer	transfer	NOUN
afs-72695	131	5	.	.	PUNCT
afs-72695	132	1	in	in	ADP
afs-72695	132	2	:	:	PUNCT
afs-72695	132	3	kung	kung	PROPN
afs-72695	132	4	,	,	PUNCT
afs-72695	132	5	s.-d	s.-d	PROPN
afs-72695	132	6	.	.	PUNCT
afs-72695	132	7	&	&	CCONJ
afs-72695	132	8	wu	wu	PROPN
afs-72695	132	9	,	,	PUNCT
afs-72695	132	10	r.	r.	PROPN
afs-72695	132	11	(	(	PUNCT
afs-72695	132	12	eds	eds	PROPN
afs-72695	132	13	.	.	PUNCT
afs-72695	132	14	)	)	PUNCT
afs-72695	132	15	.	.	PUNCT
afs-72695	133	1	transgenic	transgenic	NOUN
afs-72695	133	2	plants	plant	NOUN
afs-72695	133	3	,	,	PUNCT
afs-72695	133	4	vol	vol	NOUN
afs-72695	133	5	.	.	PUNCT
afs-72695	133	6	i.	i.	PROPN
afs-72695	133	7	academic	academic	PROPN
afs-72695	133	8	press	press	PROPN
afs-72695	133	9	,	,	PUNCT
afs-72695	133	10	inc	inc	PROPN
afs-72695	133	11	.	.	PROPN
afs-72695	133	12	,	,	PUNCT
afs-72695	133	13	san	san	PROPN
afs-72695	133	14	diego	diego	PROPN
afs-72695	133	15	,	,	PUNCT
afs-72695	133	16	california	california	PROPN
afs-72695	133	17	,	,	PUNCT
afs-72695	133	18	p.	p.	NOUN
afs-72695	133	19	125	125	NUM
afs-72695	133	20	-	-	SYM
afs-72695	133	21	147	147	NUM
afs-72695	133	22	.	.	PUNCT
afs-72695	134	1	junker	junker	PROPN
afs-72695	134	2	,	,	PUNCT
afs-72695	134	3	8	8	NUM
afs-72695	134	4	.	.	NUM
afs-72695	134	5	,	,	PUNCT
afs-72695	134	6	zimny	zimny	PROPN
afs-72695	134	7	,	,	PUNCT
afs-72695	134	8	j.	j.	PROPN
afs-72695	134	9	,	,	PUNCT
afs-72695	134	10	luhrs	luhrs	PROPN
afs-72695	134	11	,	,	PUNCT
afs-72695	134	12	r.	r.	PROPN
afs-72695	134	13	&	&	CCONJ
afs-72695	134	14	lörz	lörz	PROPN
afs-72695	134	15	,	,	PUNCT
afs-72695	134	16	h.	h.	PROPN
afs-72695	134	17	1987	1987	NUM
afs-72695	134	18	.	.	PUNCT
afs-72695	135	1	transient	transient	ADJ
afs-72695	135	2	gene	gene	NOUN
afs-72695	135	3	expression	expression	NOUN
afs-72695	135	4	of	of	ADP
afs-72695	135	5	chimeric	chimeric	ADJ
afs-72695	135	6	genes	gene	NOUN
afs-72695	135	7	in	in	ADP
afs-72695	135	8	dividing	dividing	NOUN
afs-72695	135	9	and	and	CCONJ
afs-72695	135	10	non	non	ADJ
afs-72695	135	11	-	-	ADJ
afs-72695	135	12	dividing	divide	VERB
afs-72695	135	13	cereal	cereal	NOUN
afs-72695	135	14	protoplasts	protoplast	NOUN
afs-72695	135	15	after	after	ADP
afs-72695	135	16	peg	peg	NOUN
afs-72695	135	17	-	-	PUNCT
afs-72695	135	18	induced	induce	VERB
afs-72695	135	19	dna	dna	NOUN
afs-72695	135	20	uptake	uptake	ADJ
afs-72695	135	21	.	.	PUNCT
afs-72695	136	1	plant	plant	NOUN
afs-72695	136	2	cell	cell	NOUN
afs-72695	136	3	reports	report	VERB
afs-72695	136	4	6	6	NUM
afs-72695	136	5	:	:	SYM
afs-72695	136	6	329	329	NUM
afs-72695	136	7	-	-	SYM
afs-72695	136	8	332	332	NUM
afs-72695	136	9	.	.	PUNCT
afs-72695	137	1	lazzeri	lazzeri	PROPN
afs-72695	137	2	,	,	PUNCT
afs-72695	137	3	p.	p.	NOUN
afs-72695	137	4	a.	a.	NOUN
afs-72695	137	5	,	,	PUNCT
afs-72695	137	6	brettschneider	brettschneider	PROPN
afs-72695	137	7	,	,	PUNCT
afs-72695	137	8	r.	r.	PROPN
afs-72695	137	9	,	,	PUNCT
afs-72695	137	10	luhrs	luhrs	PROPN
afs-72695	137	11	,	,	PUNCT
afs-72695	137	12	r.	r.	PROPN
afs-72695	137	13	&	&	CCONJ
afs-72695	137	14	lörz	lörz	PROPN
afs-72695	137	15	,	,	PUNCT
afs-72695	137	16	h	h	NOUN
afs-72695	137	17	,	,	PUNCT
afs-72695	137	18	1992	1992	NUM
afs-72695	137	19	.	.	PUNCT
afs-72695	138	1	stable	stable	ADJ
afs-72695	138	2	transformation	transformation	NOUN
afs-72695	138	3	of	of	ADP
afs-72695	138	4	barley	barley	NOUN
afs-72695	138	5	via	via	ADP
afs-72695	138	6	peg	peg	NOUN
afs-72695	138	7	-	-	PUNCT
afs-72695	138	8	induced	induce	VERB
afs-72695	138	9	direct	direct	ADJ
afs-72695	138	10	dna	dna	NOUN
afs-72695	138	11	uptake	uptake	ADJ
afs-72695	138	12	into	into	ADP
afs-72695	138	13	protoplasts	protoplast	NOUN
afs-72695	138	14	.	.	PUNCT
afs-72695	139	1	theoretical	theoretical	ADJ
afs-72695	139	2	and	and	CCONJ
afs-72695	139	3	applied	apply	VERB
afs-72695	139	4	genetics	genetic	NOUN
afs-72695	139	5	81	81	NUM
afs-72695	139	6	:	:	PUNCT
afs-72695	139	7	437	437	NUM
afs-72695	139	8	-	-	SYM
afs-72695	139	9	444	444	NUM
afs-72695	139	10	.	.	PUNCT
afs-72695	139	11	&	&	CCONJ
afs-72695	139	12	lörz	lörz	PROPN
afs-72695	139	13	,	,	PUNCT
afs-72695	139	14	h.	h.	PROPN
afs-72695	139	15	1990	1990	NUM
afs-72695	139	16	.	.	PUNCT
afs-72695	140	1	regenerate	regenerate	ADJ
afs-72695	140	2	suspension	suspension	NOUN
afs-72695	140	3	and	and	CCONJ
afs-72695	140	4	protoplast	protoplast	NOUN
afs-72695	140	5	cultures	culture	NOUN
afs-72695	140	6	of	of	ADP
afs-72695	140	7	barley	barley	NOUN
afs-72695	140	8	and	and	CCONJ
afs-72695	140	9	stable	stable	ADJ
afs-72695	140	10	transformation	transformation	NOUN
afs-72695	140	11	via	via	ADP
afs-72695	140	12	dna	dna	PROPN
afs-72695	140	13	uptake	uptake	ADJ
afs-72695	140	14	into	into	ADP
afs-72695	140	15	protoplasts	protoplast	NOUN
afs-72695	140	16	.	.	PUNCT
afs-72695	141	1	in	in	ADP
afs-72695	141	2	;	;	PUNCT
afs-72695	141	3	lycett	lycett	PROPN
afs-72695	141	4	,	,	PUNCT
afs-72695	141	5	g.	g.	PROPN
afs-72695	141	6	w.	w.	PROPN
afs-72695	141	7	&	&	CCONJ
afs-72695	141	8	grierson	grierson	PROPN
afs-72695	141	9	,	,	PUNCT
afs-72695	141	10	d.	d.	PROPN
afs-72695	141	11	(	(	PUNCT
afs-72695	141	12	eds	eds	PROPN
afs-72695	141	13	.	.	PUNCT
afs-72695	141	14	)	)	PUNCT
afs-72695	141	15	.	.	PUNCT
afs-72695	142	1	genetic	genetic	ADJ
afs-72695	142	2	engineering	engineering	NOUN
afs-72695	142	3	of	of	ADP
afs-72695	142	4	crop	crop	NOUN
afs-72695	142	5	plants	plant	NOUN
afs-72695	142	6	.	.	PUNCT
afs-72695	143	1	butterworth	butterworth	PROPN
afs-72695	143	2	.	.	PUNCT
afs-72695	143	3	london	london	PROPN
afs-72695	143	4	,	,	PUNCT
afs-72695	143	5	p.	p.	NOUN
afs-72695	143	6	231	231	NUM
afs-72695	143	7	-	-	SYM
afs-72695	143	8	238	238	NUM
afs-72695	143	9	,	,	PUNCT
afs-72695	143	10	luhrs	luhrs	PROPN
afs-72695	143	11	,	,	PUNCT
afs-72695	143	12	r.	r.	PROPN
afs-72695	143	13	&	&	CCONJ
afs-72695	143	14	lörz	lörz	PROPN
afs-72695	143	15	,	,	PUNCT
afs-72695	143	16	h.	h.	PROPN
afs-72695	143	17	1988	1988	NUM
afs-72695	143	18	.	.	PUNCT
afs-72695	144	1	initiation	initiation	NOUN
afs-72695	144	2	of	of	ADP
afs-72695	144	3	morphogenic	morphogenic	ADJ
afs-72695	144	4	cell	cell	NOUN
afs-72695	144	5	suspension	suspension	NOUN
afs-72695	144	6	and	and	CCONJ
afs-72695	144	7	protoplast	protoplast	NOUN
afs-72695	144	8	cultures	culture	NOUN
afs-72695	144	9	of	of	ADP
afs-72695	144	10	barley	barley	NOUN
afs-72695	144	11	(	(	PUNCT
afs-72695	144	12	hordeum	hordeum	PROPN
afs-72695	144	13	vulgare	vulgare	PROPN
afs-72695	144	14	l.	l.	PROPN
afs-72695	144	15	)	)	PUNCT
afs-72695	144	16	.	.	PUNCT
afs-72695	145	1	planta	planta	PROPN
afs-72695	145	2	175	175	NUM
afs-72695	145	3	:	:	PUNCT
afs-72695	145	4	71	71	NUM
afs-72695	145	5	-	-	SYM
afs-72695	145	6	81	81	NUM
afs-72695	145	7	.	.	PUNCT
afs-72695	146	1	mcelroy	mcelroy	PROPN
afs-72695	146	2	,	,	PUNCT
afs-72695	146	3	d	d	NOUN
afs-72695	146	4	„	„	ADJ
afs-72695	146	5	blowers	blower	NOUN
afs-72695	146	6	,	,	PUNCT
afs-72695	146	7	a.	a.	PROPN
afs-72695	146	8	,	,	PUNCT
afs-72695	146	9	jenes	jenes	PROPN
afs-72695	146	10	,	,	PUNCT
afs-72695	146	11	b.	b.	PROPN
afs-72695	146	12	&	&	CCONJ
afs-72695	146	13	wu	wu	PROPN
afs-72695	146	14	,	,	PUNCT
afs-72695	146	15	r.	r.	PROPN
afs-72695	146	16	1991	1991	NUM
afs-72695	146	17	.	.	PUNCT
afs-72695	147	1	construction	construction	NOUN
afs-72695	147	2	of	of	ADP
afs-72695	147	3	rice	rice	NOUN
afs-72695	147	4	actin-1	actin-1	PROPN
afs-72695	147	5	(	(	PUNCT
afs-72695	147	6	actl-	actl-	NUM
afs-72695	147	7	)	)	PUNCT
afs-72695	147	8	based	base	VERB
afs-72695	147	9	expression	expression	NOUN
afs-72695	147	10	vectors	vector	NOUN
afs-72695	147	11	for	for	ADP
afs-72695	147	12	use	use	NOUN
afs-72695	147	13	in	in	ADP
afs-72695	147	14	monocot	monocot	ADJ
afs-72695	147	15	transformation	transformation	NOUN
afs-72695	147	16	.	.	PUNCT
afs-72695	148	1	molecular	molecular	ADJ
afs-72695	148	2	and	and	CCONJ
afs-72695	148	3	general	general	ADJ
afs-72695	148	4	genetics	genetic	NOUN
afs-72695	148	5	231	231	NUM
afs-72695	148	6	;	;	PUNCT
afs-72695	148	7	150	150	NUM
afs-72695	148	8	-	-	SYM
afs-72695	148	9	160	160	NUM
afs-72695	148	10	.	.	PUNCT
afs-72695	149	1	menczel	menczel	PROPN
afs-72695	149	2	,	,	PUNCT
afs-72695	149	3	l.	l.	PROPN
afs-72695	149	4	,	,	PUNCT
afs-72695	149	5	nagy	nagy	PROPN
afs-72695	149	6	,	,	PUNCT
afs-72695	149	7	f.	f.	PROPN
afs-72695	149	8	,	,	PUNCT
afs-72695	149	9	kiss	kiss	VERB
afs-72695	149	10	,	,	PUNCT
afs-72695	149	11	zs	zs	PROPN
afs-72695	149	12	.	.	PROPN
afs-72695	149	13	r.	r.	PROPN
afs-72695	149	14	&	&	CCONJ
afs-72695	149	15	maliga	maliga	PROPN
afs-72695	149	16	,	,	PUNCT
afs-72695	149	17	p.	p.	NOUN
afs-72695	149	18	1981	1981	NUM
afs-72695	149	19	.	.	PUNCT
afs-72695	150	1	streptomycin	streptomycin	PROPN
afs-72695	150	2	resistant	resistant	ADJ
afs-72695	150	3	and	and	CCONJ
afs-72695	150	4	sensitive	sensitive	ADJ
afs-72695	150	5	somatic	somatic	ADJ
afs-72695	150	6	hybrids	hybrid	NOUN
afs-72695	150	7	of	of	ADP
afs-72695	150	8	nicotiana	nicotiana	NOUN
afs-72695	150	9	tabacum	tabacum	ADJ
afs-72695	150	10	tobacco	tobacco	NOUN
afs-72695	150	11	+	+	CCONJ
afs-72695	150	12	nicoliana	nicoliana	PROPN
afs-72695	150	13	knightiana	knightiana	PROPN
afs-72695	150	14	:	:	PUNCT
afs-72695	150	15	correlation	correlation	NOUN
afs-72695	150	16	of	of	ADP
afs-72695	150	17	resistance	resistance	NOUN
afs-72695	150	18	to	to	ADP
afs-72695	150	19	nicoliana	nicoliana	NOUN
afs-72695	150	20	tabacum	tabacum	ADJ
afs-72695	150	21	plastids	plastid	NOUN
afs-72695	150	22	.	.	PUNCT
afs-72695	151	1	theoretical	theoretical	ADJ
afs-72695	151	2	and	and	CCONJ
afs-72695	151	3	applied	apply	VERB
afs-72695	151	4	genetics	genetic	NOUN
afs-72695	151	5	59	59	NUM
afs-72695	151	6	:	:	SYM
afs-72695	151	7	191	191	NUM
afs-72695	151	8	-	-	SYM
afs-72695	151	9	195	195	NUM
afs-72695	151	10	.	.	PUNCT
afs-72695	152	1	mörocz	mörocz	PROPN
afs-72695	152	2	,	,	PUNCT
afs-72695	152	3	s.	s.	PROPN
afs-72695	152	4	,	,	PUNCT
afs-72695	152	5	donn	donn	PROPN
afs-72695	152	6	,	,	PUNCT
afs-72695	152	7	g.	g.	PROPN
afs-72695	152	8	,	,	PUNCT
afs-72695	152	9	németh	németh	PROPN
afs-72695	152	10	,	,	PUNCT
afs-72695	152	11	j.	j.	PROPN
afs-72695	152	12	&	&	CCONJ
afs-72695	152	13	dudits	dudits	PROPN
afs-72695	152	14	,	,	PUNCT
afs-72695	152	15	d.	d.	PROPN
afs-72695	152	16	1990	1990	NUM
afs-72695	152	17	.	.	PUNCT
afs-72695	153	1	an	an	DET
afs-72695	153	2	improved	improved	ADJ
afs-72695	153	3	system	system	NOUN
afs-72695	153	4	to	to	PART
afs-72695	153	5	obtain	obtain	VERB
afs-72695	153	6	fertile	fertile	ADJ
afs-72695	153	7	regenerants	regenerant	NOUN
afs-72695	153	8	via	via	ADP
afs-72695	153	9	maize	maize	NOUN
afs-72695	153	10	protoplasts	protoplast	NOUN
afs-72695	153	11	isolated	isolate	VERB
afs-72695	153	12	from	from	ADP
afs-72695	153	13	a	a	DET
afs-72695	153	14	highly	highly	ADV
afs-72695	153	15	embryogenic	embryogenic	ADJ
afs-72695	153	16	suspension	suspension	NOUN
afs-72695	153	17	culture	culture	NOUN
afs-72695	153	18	.	.	PUNCT
afs-72695	154	1	theoretical	theoretical	ADJ
afs-72695	154	2	applied	apply	VERB
afs-72695	154	3	genetics	genetic	NOUN
afs-72695	154	4	80	80	NUM
afs-72695	154	5	:	:	PUNCT
afs-72695	154	6	721	721	NUM
afs-72695	154	7	-	-	SYM
afs-72695	154	8	726	726	NUM
afs-72695	154	9	.	.	PUNCT
afs-72695	154	10	pauk	pauk	NOUN
afs-72695	154	11	,	,	PUNCT
afs-72695	154	12	j.	j.	PROPN
afs-72695	154	13	,	,	PUNCT
afs-72695	154	14	kertesz	kertesz	PROPN
afs-72695	154	15	,	,	PUNCT
afs-72695	154	16	z.	z.	PROPN
afs-72695	154	17	,	,	PUNCT
afs-72695	154	18	jenes	jenes	PROPN
afs-72695	154	19	,	,	PUNCT
afs-72695	154	20	8	8	NUM
afs-72695	154	21	.	.	NUM
afs-72695	154	22	,	,	PUNCT
afs-72695	154	23	purnhauser	purnhauser	PROPN
afs-72695	154	24	,	,	PUNCT
afs-72695	154	25	l.	l.	PROPN
afs-72695	154	26	,	,	PUNCT
afs-72695	154	27	manninen	manninen	NOUN
afs-72695	154	28	,	,	PUNCT
afs-72695	154	29	0	0	NUM
afs-72695	154	30	.	.	NUM
afs-72695	154	31	,	,	PUNCT
afs-72695	154	32	pulu	pulu	PROPN
afs-72695	154	33	.	.	PROPN
afs-72695	154	34	,	,	PUNCT
afs-72695	154	35	barabas	barabas	PROPN
afs-72695	154	36	,	,	PUNCT
afs-72695	154	37	z.	z.	PROPN
afs-72695	154	38	&	&	CCONJ
afs-72695	154	39	dudits	dudits	PROPN
afs-72695	154	40	,	,	PUNCT
afs-72695	154	41	d.	d.	PROPN
afs-72695	154	42	1994	1994	NUM
afs-72695	154	43	.	.	PUNCT
afs-72695	155	1	fertile	fertile	ADJ
afs-72695	155	2	wheat	wheat	NOUN
afs-72695	155	3	(	(	PUNCT
afs-72695	155	4	triticum	triticum	NOUN
afs-72695	155	5	aestivum	aestivum	PROPN
afs-72695	155	6	l.	l.	PROPN
afs-72695	155	7	)	)	PUNCT
afs-72695	155	8	regenerants	regenerant	NOUN
afs-72695	155	9	from	from	ADP
afs-72695	155	10	protoplasts	protoplast	NOUN
afs-72695	155	11	of	of	ADP
afs-72695	155	12	embryogenic	embryogenic	ADJ
afs-72695	155	13	suspension	suspension	NOUN
afs-72695	155	14	culture	culture	NOUN
afs-72695	155	15	.	.	PUNCT
afs-72695	156	1	plant	plant	NOUN
afs-72695	156	2	cell	cell	NOUN
afs-72695	156	3	,	,	PUNCT
afs-72695	156	4	tissue	tissue	NOUN
afs-72695	156	5	and	and	CCONJ
afs-72695	156	6	organ	organ	NOUN
afs-72695	156	7	culture	culture	NOUN
afs-72695	156	8	.	.	PUNCT
afs-72695	157	1	in	in	ADP
afs-72695	157	2	press	press	NOUN
afs-72695	157	3	.	.	PUNCT
afs-72695	158	1	shimamoto	shimamoto	PROPN
afs-72695	158	2	,	,	PUNCT
afs-72695	158	3	k.	k.	PROPN
afs-72695	158	4	,	,	PUNCT
afs-72695	158	5	terada	terada	PROPN
afs-72695	158	6	,	,	PUNCT
afs-72695	158	7	r.	r.	PROPN
afs-72695	158	8	,	,	PUNCT
afs-72695	158	9	izawa	izawa	PROPN
afs-72695	158	10	,	,	PUNCT
afs-72695	158	11	t.	t.	PROPN
afs-72695	158	12	&	&	CCONJ
afs-72695	158	13	fujimoto	fujimoto	PROPN
afs-72695	158	14	,	,	PUNCT
afs-72695	158	15	h.	h.	PROPN
afs-72695	158	16	1989	1989	NUM
afs-72695	158	17	.	.	PUNCT
afs-72695	159	1	fertile	fertile	ADJ
afs-72695	159	2	transgenic	transgenic	NOUN
afs-72695	159	3	rice	rice	NOUN
afs-72695	159	4	plants	plant	NOUN
afs-72695	159	5	regenerated	regenerate	VERB
afs-72695	159	6	from	from	ADP
afs-72695	159	7	tranformed	tranforme	VERB
afs-72695	159	8	protoplasts	protoplast	NOUN
afs-72695	159	9	.	.	PUNCT
afs-72695	160	1	nature	nature	NOUN
afs-72695	160	2	338	338	NUM
afs-72695	160	3	:	:	PUNCT
afs-72695	160	4	274	274	NUM
afs-72695	160	5	-	-	SYM
afs-72695	160	6	277	277	NUM
afs-72695	160	7	.	.	PUNCT
afs-72695	161	1	skriver	skriver	PROPN
afs-72695	161	2	,	,	PUNCT
afs-72695	161	3	k.	k.	PROPN
afs-72695	161	4	,	,	PUNCT
afs-72695	161	5	olsen	olsen	PROPN
afs-72695	161	6	,	,	PUNCT
afs-72695	161	7	s.l	s.l	PROPN
afs-72695	161	8	.	.	PROPN
afs-72695	161	9	,	,	PUNCT
afs-72695	161	10	rogers	rogers	PROPN
afs-72695	161	11	,	,	PUNCT
afs-72695	161	12	j.c	j.c	PROPN
afs-72695	161	13	,	,	PUNCT
afs-72695	161	14	&	&	CCONJ
afs-72695	161	15	mundy	mundy	PROPN
afs-72695	161	16	,	,	PUNCT
afs-72695	161	17	j	j	PROPN
afs-72695	161	18	,	,	PUNCT
afs-72695	161	19	1991	1991	NUM
afs-72695	161	20	.	.	PUNCT
afs-72695	162	1	cw	cw	ADJ
afs-72695	162	2	-	-	ADJ
afs-72695	162	3	acting	acting	ADJ
afs-72695	162	4	dna	dna	NOUN
afs-72695	162	5	elements	element	NOUN
afs-72695	162	6	responsive	responsive	ADJ
afs-72695	162	7	to	to	ADP
afs-72695	162	8	gibberellin	gibberellin	NOUN
afs-72695	162	9	and	and	CCONJ
afs-72695	162	10	its	its	PRON
afs-72695	162	11	antagonist	antagonist	NOUN
afs-72695	162	12	abscisic	abscisic	ADJ
afs-72695	162	13	acid	acid	NOUN
afs-72695	162	14	.	.	PUNCT
afs-72695	163	1	proceedings	proceeding	NOUN
afs-72695	163	2	of	of	ADP
afs-72695	163	3	the	the	DET
afs-72695	163	4	national	national	PROPN
afs-72695	163	5	academy	academy	PROPN
afs-72695	163	6	of	of	ADP
afs-72695	163	7	sciences	sciences	PROPN
afs-72695	163	8	.	.	PUNCT
afs-72695	164	1	usa	usa	PROPN
afs-72695	164	2	.	.	PROPN
afs-72695	164	3	vol	vol	NOUN
afs-72695	164	4	88	88	NUM
afs-72695	164	5	:	:	PUNCT
afs-72695	164	6	7266	7266	NUM
afs-72695	164	7	-	-	SYM
afs-72695	164	8	7270	7270	NUM
afs-72695	164	9	.	.	PUNCT
afs-72695	165	1	thompson	thompson	PROPN
afs-72695	165	2	,	,	PUNCT
afs-72695	165	3	j.	j.	PROPN
afs-72695	165	4	a.	a.	PROPN
afs-72695	165	5	,	,	PUNCT
afs-72695	165	6	abdullah	abdullah	PROPN
afs-72695	165	7	,	,	PUNCT
afs-72695	165	8	r.	r.	PROPN
afs-72695	165	9	&	&	CCONJ
afs-72695	165	10	cocking	cocking	PROPN
afs-72695	165	11	,	,	PUNCT
afs-72695	165	12	e.	e.	PROPN
afs-72695	165	13	c.	c.	PROPN
afs-72695	165	14	1986	1986	NUM
afs-72695	165	15	.	.	PUNCT
afs-72695	166	1	protoplast	protoplast	NOUN
afs-72695	166	2	culture	culture	NOUN
afs-72695	166	3	of	of	ADP
afs-72695	166	4	rice	rice	NOUN
afs-72695	166	5	(	(	PUNCT
afs-72695	166	6	oryza	oryza	PROPN
afs-72695	166	7	saliva	saliva	PROPN
afs-72695	166	8	l.	l.	PROPN
afs-72695	166	9	)	)	PUNCT
afs-72695	166	10	using	use	VERB
afs-72695	166	11	media	medium	NOUN
afs-72695	166	12	solidified	solidify	VERB
afs-72695	166	13	with	with	ADP
afs-72695	166	14	agarose	agarose	NOUN
afs-72695	166	15	.	.	PUNCT
afs-72695	167	1	plant	plant	NOUN
afs-72695	167	2	science	science	NOUN
afs-72695	167	3	47	47	NUM
afs-72695	167	4	:	:	SYM
afs-72695	167	5	123133	123133	NUM
afs-72695	167	6	.	.	PUNCT
afs-72695	168	1	vasil	vasil	NOUN
afs-72695	168	2	,	,	PUNCT
afs-72695	168	3	v.	v.	PROPN
afs-72695	168	4	,	,	PUNCT
afs-72695	168	5	redway	redway	PROPN
afs-72695	168	6	,	,	PUNCT
afs-72695	168	7	f.	f.	PROPN
afs-72695	168	8	&	&	CCONJ
afs-72695	168	9	vasil	vasil	PROPN
afs-72695	168	10	,	,	PUNCT
afs-72695	168	11	i.k	i.k	PROPN
afs-72695	168	12	.	.	PROPN
afs-72695	168	13	1990	1990	NUM
afs-72695	168	14	.	.	PUNCT
afs-72695	169	1	regeneration	regeneration	NOUN
afs-72695	169	2	of	of	ADP
afs-72695	169	3	plants	plant	NOUN
afs-72695	169	4	from	from	ADP
afs-72695	169	5	embryogenic	embryogenic	ADJ
afs-72695	169	6	suspension	suspension	NOUN
afs-72695	169	7	culture	culture	NOUN
afs-72695	169	8	protoplasts	protoplast	NOUN
afs-72695	169	9	of	of	ADP
afs-72695	169	10	wheat	wheat	NOUN
afs-72695	169	11	(	(	PUNCT
afs-72695	169	12	triticum	triticum	NOUN
afs-72695	169	13	aestivum	aestivum	PROPN
afs-72695	169	14	l.).8i0	l.).8i0	PROPN
afs-72695	169	15	/	/	SYM
afs-72695	169	16	technology	technology	NOUN
afs-72695	169	17	8	8	NUM
afs-72695	169	18	:	:	SYM
afs-72695	169	19	429	429	NUM
afs-72695	169	20	-	-	SYM
afs-72695	169	21	134	134	NUM
afs-72695	169	22	.	.	PUNCT
afs-72695	170	1	yang	yang	PROPN
afs-72695	170	2	,	,	PUNCT
afs-72695	170	3	y.m	y.m	PROPN
afs-72695	170	4	.	.	PROPN
afs-72695	170	5	,	,	PUNCT
afs-72695	170	6	he	he	PRON
afs-72695	170	7	,	,	PUNCT
afs-72695	170	8	d.d	d.d	PROPN
afs-72695	170	9	.	.	PROPN
afs-72695	170	10	&	&	CCONJ
afs-72695	170	11	scott	scott	PROPN
afs-72695	170	12	,	,	PUNCT
afs-72695	170	13	k.j	k.j	PROPN
afs-72695	170	14	.	.	PROPN
afs-72695	170	15	1993	1993	NUM
afs-72695	170	16	.	.	PUNCT
afs-72695	171	1	plant	plant	NOUN
afs-72695	171	2	regeneration	regeneration	NOUN
afs-72695	171	3	from	from	ADP
afs-72695	171	4	protoplasts	protoplast	NOUN
afs-72695	171	5	of	of	ADP
afs-72695	171	6	durum	durum	ADJ
afs-72695	171	7	wheat	wheat	NOUN
afs-72695	171	8	(	(	PUNCT
afs-72695	171	9	triticum	triticum	NOUN
afs-72695	171	10	aestivum	aestivum	ADJ
afs-72695	171	11	desf	desf	NOUN
afs-72695	171	12	.	.	PUNCT
afs-72695	171	13	,	,	PUNCT
afs-72695	171	14	cv	cv	PROPN
afs-72695	171	15	.	.	PUNCT
afs-72695	171	16	d6962	d6962	PROPN
afs-72695	171	17	)	)	PUNCT
afs-72695	171	18	.	.	PUNCT
afs-72695	172	1	plant	plant	NOUN
afs-72695	172	2	cell	cell	NOUN
afs-72695	172	3	reports	report	VERB
afs-72695	172	4	12	12	NUM
afs-72695	172	5	:	:	SYM
afs-72695	172	6	320323	320323	NUM
afs-72695	172	7	.	.	PUNCT
afs-72695	173	1	zhang	zhang	PROPN
afs-72695	173	2	,	,	PUNCT
afs-72695	173	3	w.	w.	PROPN
afs-72695	173	4	,	,	PUNCT
afs-72695	173	5	mcelroy	mcelroy	PROPN
afs-72695	173	6	,	,	PUNCT
afs-72695	173	7	d.	d.	PROPN
afs-72695	173	8	&	&	CCONJ
afs-72695	173	9	wu	wu	PROPN
afs-72695	173	10	,	,	PUNCT
afs-72695	173	11	r.	r.	PROPN
afs-72695	173	12	1991	1991	NUM
afs-72695	173	13	.	.	PUNCT
afs-72695	174	1	analysis	analysis	NOUN
afs-72695	174	2	of	of	ADP
afs-72695	174	3	rice	rice	NOUN
afs-72695	174	4	actl	actl	NOUN
afs-72695	174	5	5	5	NUM
afs-72695	174	6	’	'	PUNCT
afs-72695	174	7	region	region	NOUN
afs-72695	174	8	activity	activity	NOUN
afs-72695	174	9	in	in	ADP
afs-72695	174	10	transgenic	transgenic	NOUN
afs-72695	174	11	rice	rice	NOUN
afs-72695	174	12	plants	plant	NOUN
afs-72695	174	13	.	.	PUNCT
afs-72695	175	1	plant	plant	NOUN
afs-72695	175	2	cell	cell	NOUN
afs-72695	175	3	3	3	NUM
afs-72695	175	4	:	:	SYM
afs-72695	175	5	1155	1155	NUM
afs-72695	175	6	-	-	SYM
afs-72695	175	7	1165	1165	NUM
afs-72695	175	8	.	.	PUNCT
afs-72695	176	1	manuscript	manuscript	NOUN
afs-72695	176	2	received	receive	VERB
afs-72695	176	3	january	january	PROPN
afs-72695	176	4	1994	1994	NUM
afs-72695	176	5	204	204	NUM
afs-72695	176	6	agricultural	agricultural	ADJ
afs-72695	176	7	science	science	NOUN
afs-72695	176	8	in	in	ADP
afs-72695	176	9	finland	finland	PROPN
afs-72695	176	10	3	3	NUM
afs-72695	176	11	(	(	PUNCT
afs-72695	176	12	1994	1994	NUM
afs-72695	176	13	)	)	PUNCT
afs-72695	176	14	research	research	NOUN
afs-72695	176	15	note	note	NOUN
afs-72695	176	16	selostus	selostus	NOUN
afs-72695	176	17	nopea	nopea	PROPN
afs-72695	176	18	menetelmä	menetelmä	PROPN
afs-72695	176	19	ohran	ohran	PROPN
afs-72695	176	20	protoplastien	protoplastien	PROPN
afs-72695	176	21	eristämiseksi	eristämiseksi	NOUN
afs-72695	176	22	ja	ja	PROPN
afs-72695	176	23	sen	sen	PROPN
afs-72695	176	24	soveltaminen	soveltaminen	PROPN
afs-72695	176	25	geeninsiirtoon	geeninsiirtoon	PROPN
afs-72695	176	26	barnabas	barnabas	PROPN
afs-72695	176	27	jenes	jenes	PROPN
afs-72695	176	28	,	,	PUNCT
afs-72695	176	29	matti	matti	PROPN
afs-72695	176	30	puolimatka	puolimatka	PROPN
afs-72695	176	31	,	,	PUNCT
afs-72695	176	32	pedro	pedro	PROPN
afs-72695	176	33	bittencourt	bittencourt	PROPN
afs-72695	176	34	ja	ja	PROPN
afs-72695	176	35	seppo	seppo	PROPN
afs-72695	176	36	pulli	pulli	PROPN
afs-72695	176	37	agricultural	agricultural	PROPN
afs-72695	176	38	biotechnology	biotechnology	PROPN
afs-72695	176	39	center	center	PROPN
afs-72695	176	40	ja	ja	PROPN
afs-72695	176	41	maatalouden	maatalouden	PROPN
afs-72695	176	42	tutkimuskeskus	tutkimuskesku	VERB
afs-72695	176	43	tutkimuksessa	tutkimuksessa	PROPN
afs-72695	176	44	kehitettiin	kehitettiin	NOUN
afs-72695	176	45	menetelmä	menetelmä	ADJ
afs-72695	176	46	,	,	PUNCT
afs-72695	176	47	jossa	jossa	PROPN
afs-72695	176	48	protoplasteja	protoplasteja	PROPN
afs-72695	176	49	eristetään	eristetään	PROPN
afs-72695	176	50	suoraan	suoraan	PROPN
afs-72695	176	51	ohran	ohran	PROPN
afs-72695	176	52	jyvien	jyvien	PROPN
afs-72695	176	53	alkion	alkion	PROPN
afs-72695	176	54	ja	ja	PROPN
afs-72695	176	55	sirkkakilven	sirkkakilven	PROPN
afs-72695	176	56	solukosta	solukosta	PROPN
afs-72695	176	57	ilman	ilman	PROPN
afs-72695	176	58	edeltävää	edeltävää	ADJ
afs-72695	176	59	solukkoviljelyvaihetta	solukkoviljelyvaihetta	PROPN
afs-72695	176	60	.	.	PUNCT
afs-72695	177	1	jyvät	jyvät	PROPN
afs-72695	177	2	pintasteriloitiin	pintasteriloitiin	PROPN
afs-72695	177	3	ja	ja	PROPN
afs-72695	177	4	niitä	niitä	ADV
afs-72695	177	5	liuotettiin	liuotettiin	PROPN
afs-72695	177	6	steriloidussa	steriloidussa	ADJ
afs-72695	177	7	vedessä	vedessä	NOUN
afs-72695	177	8	vuorokausi	vuorokausi	NOUN
afs-72695	177	9	.	.	PUNCT
afs-72695	178	1	tämän	tämän	PROPN
afs-72695	178	2	jälkeen	jälkeen	PROPN
afs-72695	178	3	alkion	alkion	PROPN
afs-72695	178	4	ja	ja	PROPN
afs-72695	178	5	sirkkakilven	sirkkakilven	PROPN
afs-72695	178	6	solukot	solukot	PROPN
afs-72695	178	7	eristettiin	eristettiin	PROPN
afs-72695	178	8	jyvistä	jyvistä	PROPN
afs-72695	178	9	ja	ja	PROPN
afs-72695	178	10	pilkottiin	pilkottiin	PROPN
afs-72695	178	11	pieniksi	pieniksi	PROPN
afs-72695	178	12	paloiksi	paloiksi	PROPN
afs-72695	178	13	.	.	PUNCT
afs-72695	179	1	protoplastien	protoplastien	NOUN
afs-72695	179	2	eristämiseksi	eristämiseksi	NOUN
afs-72695	179	3	kokeiltiin	kokeiltiin	NOUN
afs-72695	179	4	aluksi	aluksi	PROPN
afs-72695	179	5	kolmea	kolmea	PROPN
afs-72695	179	6	erilaista	erilaista	PROPN
afs-72695	179	7	entsyymiliuosta	entsyymiliuosta	PROPN
afs-72695	179	8	,	,	PUNCT
afs-72695	179	9	joista	joista	ADJ
afs-72695	179	10	paras	paras	ADJ
afs-72695	179	11	valittiin	valittiin	NOUN
afs-72695	179	12	peg	peg	NOUN
afs-72695	179	13	(	(	PUNCT
afs-72695	179	14	polyetyleeniglykoli)menetelmällä	polyetyleeniglykoli)menetelmällä	NOUN
afs-72695	179	15	tehtyihin	tehtyihin	ADJ
afs-72695	179	16	geeninsiirtokokeisiin	geeninsiirtokokeisiin	NOUN
afs-72695	179	17	.	.	PUNCT
afs-72695	180	1	siirtokokeissa	siirtokokeissa	ADJ
afs-72695	180	2	käytetty	käytetty	PROPN
afs-72695	180	3	rengasmainen	rengasmainen	NOUN
afs-72695	180	4	plasmidi	plasmidi	PROPN
afs-72695	180	5	-	-	PUNCT
afs-72695	180	6	dna	dna	NOUN
afs-72695	180	7	pactl	pactl	NOUN
afs-72695	180	8	-	-	PUNCT
afs-72695	180	9	f	f	PROPN
afs-72695	180	10	sisälsi	sisälsi	PROPN
afs-72695	180	11	riisin	riisin	PROPN
afs-72695	180	12	acf/-säätelyjakson	acf/-säätelyjakson	PROPN
afs-72695	180	13	,	,	PUNCT
afs-72695	180	14	cics	cic	NOUN
afs-72695	180	15	-	-	PUNCT
afs-72695	180	16	geenin	geenin	NOUN
afs-72695	180	17	(	(	PUNCT
afs-72695	180	18	koodaa	koodaa	NOUN
afs-72695	180	19	p	p	NOUN
afs-72695	180	20	-	-	PUNCT
afs-72695	180	21	glukuronidaasi	glukuronidaasi	NOUN
afs-72695	180	22	-	-	PUNCT
afs-72695	180	23	entsyymiä	entsyymiä	NOUN
afs-72695	180	24	)	)	PUNCT
afs-72695	180	25	ja	ja	PROPN
afs-72695	180	26	afav	afav	PROPN
afs-72695	180	27	-	-	PUNCT
afs-72695	180	28	lopetusjakson	lopetusjakson	PROPN
afs-72695	180	29	.	.	PUNCT
afs-72695	181	1	peg	peg	NOUN
afs-72695	181	2	-	-	PUNCT
afs-72695	181	3	käsittelyn	käsittelyn	NOUN
afs-72695	181	4	jälkeen	jälkeen	PROPN
afs-72695	181	5	protoplasteja	protoplasteja	PROPN
afs-72695	181	6	kasvatettiin	kasvatettiin	PROPN
afs-72695	181	7	kpr	kpr	PROPN
afs-72695	181	8	-	-	PUNCT
afs-72695	181	9	kasvatusalustalla	kasvatusalustalla	PROPN
afs-72695	181	10	.	.	PUNCT
afs-72695	182	1	transienttinen	transienttinen	NOUN
afs-72695	182	2	(	(	PUNCT
afs-72695	182	3	väliaikainen	väliaikainen	NOUN
afs-72695	182	4	)	)	PUNCT
afs-72695	182	5	gu.v	gu.v	PROPN
afs-72695	182	6	-	-	PUNCT
afs-72695	182	7	geenin	geenin	PROPN
afs-72695	182	8	ilmentyminen	ilmentyminen	PROPN
afs-72695	182	9	määritettiin	määritettiin	PROPN
afs-72695	182	10	vuorokauden	vuorokauden	PROPN
afs-72695	182	11	kuluttua	kuluttua	PROPN
afs-72695	182	12	käsittelemällä	käsittelemällä	PROPN
afs-72695	182	13	transformoituja	transformoituja	PROPN
afs-72695	182	14	protoplasteja	protoplasteja	PROPN
afs-72695	182	15	x	x	NOUN
afs-72695	182	16	-	-	ADJ
afs-72695	182	17	gluc	gluc	NOUN
afs-72695	182	18	-	-	PUNCT
afs-72695	182	19	entsyymisubstraatilla	entsyymisubstraatilla	NOUN
afs-72695	182	20	.	.	PUNCT
afs-72695	183	1	enimmillään	enimmillään	PROPN
afs-72695	183	2	noin	noin	PROPN
afs-72695	183	3	kuudessa	kuudessa	PROPN
afs-72695	183	4	prosentissa	prosentissa	PROPN
afs-72695	183	5	transformoiduista	transformoiduista	PROPN
afs-72695	183	6	protoplasteista	protoplasteista	PROPN
afs-72695	183	7	havaittiin	havaittiin	VERB
afs-72695	183	8	gus	gus	PROPN
afs-72695	183	9	-	-	PUNCT
afs-72695	183	10	geenin	geenin	PROPN
afs-72695	183	11	ilmentymistä	ilmentymistä	NOUN
afs-72695	183	12	.	.	PUNCT
afs-72695	184	1	tulosten	tulosten	PROPN
afs-72695	184	2	mukaan	mukaan	PROPN
afs-72695	184	3	tämä	tämä	PROPN
afs-72695	184	4	nopea	nopea	PROPN
afs-72695	184	5	protoplastien	protoplastien	PROPN
afs-72695	184	6	eristystekniikka	eristystekniikka	VERB
afs-72695	184	7	soveltuu	soveltuu	PROPN
afs-72695	184	8	erityisesti	erityisesti	NOUN
afs-72695	184	9	väliaikaisen	väliaikaisen	ADJ
afs-72695	184	10	geeni	geeni	PROPN
afs-72695	184	11	-	-	PUNCT
afs-72695	184	12	ilmentymisen	ilmentymisen	PROPN
afs-72695	184	13	tutkimukseen	tutkimukseen	PROPN
afs-72695	184	14	,	,	PUNCT
afs-72695	184	15	sillä	sillä	VERB
afs-72695	184	16	ajansäästö	ajansäästö	PROPN
afs-72695	184	17	protoplastien	protoplastien	PROPN
afs-72695	184	18	normaaliin	normaaliin	PROPN
afs-72695	184	19	suspensioviljelmään	suspensioviljelmään	PROPN
afs-72695	184	20	verrattuna	verrattuna	PROPN
afs-72695	184	21	on	on	ADP
afs-72695	184	22	huomattava	huomattava	NOUN
afs-72695	184	23	.	.	PUNCT
afs-72695	185	1	205	205	NUM
afs-72695	185	2	agricultural	agricultural	ADJ
afs-72695	185	3	science	science	NOUN
afs-72695	185	4	in	in	ADP
afs-72695	185	5	finland	finland	PROPN
afs-72695	185	6	3	3	NUM
afs-72695	185	7	(	(	PUNCT
afs-72695	185	8	1994	1994	NUM
afs-72695	185	9	)	)	PUNCT