INFLUENCE OF LEGUMES ON MICROBIAL ACTIVITY IN SOIL By Armi Kaila Institute of Agricultural Chemistry, University of Helsinki. Received Bth September 1951. The influence higher plants are known to exert upon the development of soil microflora is of the highest importance. The abundance and quality of the micro- scopic population in the soil has been found to depend more on the crop than on the moisture, temperature and acidity of their environment, provided these factors vary within natural ranges (5). Even the so called seasonal fluctuation of micro- organisms in the soil is attributed to the development of the higher plants (14). Plants modify the soil structure by the penetration of their roots, thus causing changes in the aeration and moisture conditions of the soil. They withdraw mineral nutrients from soil, and excrete carbon dioxide and various organic compounds, and after their death large amounts of organic matter remains in the soil. All this affects the development of soil microorganisms, and since both the activities of various plants and the amount and quality of their residues vary, the population harbouring the soil under and after each crop has its own characteristics. There is some evidence that the rhizosphere of the legumes affords especially favourable conditions for the development of an abundant and active microflora. In order to elucidate the influence of legumes upon the microbial population and its activity in the soil, some experiments were conducted in connection with some other investigations. The annual legumes, field pea and vetch, were compared with oats, both as single and mixed crops. Red clover represented ley legumes, and its influence upon the soil microflora was investigated in comparison with that of timothy. Materials and methods. Material for this investigation was collected from the field experiments of the Agricultural Research Centre in the neighbourhood of Helsinki, during the summers 1949 and 1950. The main part of the material was taken in the latter year. The experiments sampled were the following: https://www.c-info.fi/en/info/?token=KGbhd56MXKyYfQNk.hVrbu_xbcpS6ubjjgOHdtA.-f_7UuAZuCezIkGVkQg4eTJtVMT6YKgrX0b40gEzOAZEx2PD5r-SeJAI2mFs7WqxBbhtBp8rZI1EO2SjXawc2QH95ExNmpG0KiuLwageSVrdqg6awarPWBMCxdd7Tbjm981Y1Qdo9yBTP079ym7SBhQMaIqGSXAU 212 ARMI KAILA Experiment S I: Contained plots of oats, of mixed oats and pea (ratio in the seed 50 : 50), of pea, of mixed oats and vetch (ratio in the seed 50 : 50), and of vetch. Experiment S II: Residual effect of crops similar to those in experiment S I; crop: barley. Experiment N I: Contained plots of timothy, of timothy and red clover (ratio in the seed 10: 15), and of red clover. Plants were sown the previous spring. Experiment N II: Residual effect of ley plants, similar to those in experiment N I; crop: summer wheat. The soil in all these experiments was finesand clay. In all the experiments the plots received normal amounts of phosphorus and potassium fertilizers as spring dressings. Nitrogen was applied only to timothy plots of experiment N I at the beginning of May. Soil samples for the various investigations were taken with a bore, six or eight borings from each plot. The samples were taken down to the depth of the ploughing layer. Samples from the four or five replicate plots were combined and thoroughly mixed. Fresh samples were used for the experiments. The rhizosphere samples were taken using the technique of Wallace and Lochhead (18): The root systems of ten plants of every kind were collected, shaken as free as possible of soil and placed in 100 ml of sterile tap water. After thorough mixing, inoculations were made. The abundance of bacteria in the rhizospheres was estimated by common plate count using yeastwater-soilextract agar, mineral agar containing aspartic acid or ammonium sulfate as a source of nitrogen, and nitrogen-free mannitol agar. Eight replicates of each were made, and the Petri dishes were incubated for 5 days at 26°C. The tests for nitrogen fixation were per- formed using nitrogen-free mannitol solution inoculated with the root suspensions. The nitrogen was determined by the common Kjeldahl procedure. The increase in the nitrate-nitrogen content of soil samples after an incubation period of four weeks was taken to indicate the intensity of nitrification. The nitrate- nitrogen was determined by the somewhat modified method of Berge (1). The rate of soil respiration was estimated by collecting in sodium hydroxide solution the carbon dioxide evolved from soil incubated in big desiccators. At the moment there is no simple method for testing the significance of the differences between such figures as those obtained in this investigation through subtraction to represent the amounts of nitrified nitrogen or of nitrogen fixed. In absence of anything better, the supposition was made that these values represent means for samples drawn from populations with the same variance, and the prob- ability tables for normal distribution were applied (cf. 12, p. 80). Since it seemed justifiable to assume that the variation in the results of the replicate estimations arises from analytical errors, the standard deviation for the means of the replicate observations was calculated from the material of the whole experiment (cf. 3, pp. 209—211). INFLUENCE OF LEGUMES ON MICROBIAL ACTIVITY IN SOIL 213 Observations about the leguminous and non-leguminous rhizosphere microflora. Several authors report larger numbers of bacteria in the rhizospheres of legumes as compared with those of non-legumes (4, 6,9, 16). The species most frequently stated as being favoured by legume roots are members of the Radiobacter group (9, 15). In recent investigations Canadian microbiologists noted differences in the nutritional quality of the rhizosphere flora of legumes and non-legumes (18, 19). They found that legumes, particularly, appear to exert a pronounced effect in stimulating significantly the bacteria of those groups which require only mineral nutrients, or simple amino acids, or amino acids and growth factors. The authors consider this to be of interest in connection with the demonstration of amino acids excretion by the leguminous plants. Within the bounds of this investigation some simple tests were made to ascertain the abundance and quality of the rhizosphere population of annual legumes and non-legumes. In summer 1949, plate counts of bacteria growing on various agar media were made from the rhizosphere of oat, pea, and vetch plants at three diffe- rent stages of growth. The results, reported in Table 1, arc calculated as millions per ten plants, as this is supposed to give a more accurate picture of the density of the microbes than figures on the number of organisms per gram of root dry matter, or per gram of adhering soil. The data can be taken to give the magnitude class of the microbes approximately only. No colonies appeared on the nitrogen free mannitol agar. At the beginning of the growing season the pea plants appear to support a more abundant bacterial flora than the vetch or the oat plants. Later in the summer, oat roots reach the microbial density of pea roots, but at the end of the growing season pea plants show their superiority again. The rhizosphere flora of vetch growing on these media, remains poor through the whole season. Table 1. Abundance of bacteria in the rhizosphere of oat, pea, and vetch plants (Expressed as millions per ten plants). Weight of Bacteria developing upon Date of yeast water . , ammonium ~ Plant roots adhering soil ' aspartic acidsamplings soil extract sulfate g. g agar agar agar June 10. Oat 0.48 1.07 150 300 60 Pea 0.61 1.36 1200 1200 400 Vetch 0.55 1.79 700 300 20 July 20. Oal 3.00 4.40 LOOOO 4000 7000 Pea 1.82 3.34 2000 6000 4000 Vetch 1.62 4.30 1000 400 1000 August 30. Oat 0.68 1.06 1500 4000 2000 Pea 0.89 1.18 2500 8500 7000 Vetch 0.60 0.62 1500 2500 100 ARMI KAILA214 Table 2. Increase upon incubation in the nitrogen content of solution cultures of rhizosphere mic flora from oat, pea, and vetch plants. (Expressed as milligrams of nitrogen per culture flask). Period of incubation Oat Pea Vetch June 10. July 4. 0.26 2.12 0.07 July 20. August 12. 3.17 2.92 3.21 August 30. September 20. 1.02 0.25 0.63 Value required for significant difference at 5 per cent level 0.38 The results obtained on aspartic acid agar deserve most attention. The explan- ation of the large number of organisms from pea rhizosphere, found at the lasi samplirg may lie in the fact that among the plants there were some fresh ones with an abundance of effective nodules. The nodules in the earlier samplings were nearly all colourless, and, hence, inactive (17). Thus this figure may be taken to agree with the speculations of Wallace and Lochhead, mentioned above. Even the number of colonies developed on the ammonia sulfate agar media from the pea roots flora of this sampling was great, in accordance with the finding of these investigators. Earlier papers contain evidence that non-symbiotic nitrogen-fixing bacteria especially are stimulated in their development by legumes (9). Starkey (14), however, reports that legumes had no more effect on the non-symbiotic nitrogen- fixing organisms than did non-legumes. Clark (2) points out in his review that at present neither adequate microbiological data nor acceptable crop vield data exist to warrant a positive statement to the effect that increased fixation of atmospheric nitrogen occurs in the rhizosphere of legumes or non-legumes. The tests for nitrogen fixation in nitrogen-free mannitol solution media, per- formed with the same root material as the plate counts reported above, did not in general prove any superiority of the legumes over the non-legume (Table 2). Only in the first test the increase in the nitrogen content of the cultures inoculated with microflora from pea rhizosphere exceeds that of the other cultures. The nitrogen fixation may be attributed to the activity of anaerobic bacteria, since no sign of the occurence of Azotobacter was to be found, and according to other investigations, Clostridium species are known to be common in these soils. It is perhaps too much to imagine that the nitrogen fixation ability of the flora of the young pea plants could be attributed to the stronger utilization of oxygen by the roots, which would create conditions favourable for the anaerobic Clostridium population. Undoubtedly, the importance of the rhizosphere microflora for the host-plant is considerable, but its effect on soil conditions may be less marked, due to the fact that the microscopic population stimulated by the plant roots loses its special quality soon after the death of the plants (14). In addition, the sphere of domination of the characteristic rhizosphere flora does not extend far from the root surface region (15). Therefore, as regards soil fertility, it is probably more necessary to investigate whether the various crops affect microbial activities in the soil as a whole. INFLUENCE OF LEGUMES ON MICROBIAL ACTIVITY IN SOIL 215 Table 3. Increase upon incubation in the nitrate-nitrogen content of soil samples from plots growing annual legume and non-legume crops (Expressed as N03-N p.p.m. of soil dry matter). Experiment SI. Crop Date of samplings Qats Mixed pea pea Mixed vetch and oats and oats In 1949 June 8. 17.3 12.9 23.1 26.7 29.3 August 1. 23.2 19.8 34.9 24.6 29.7 September 13. 17.6 13.6 20.7 17.4 18.7 October 24. 12.6 11.6 16.1 17.9 17.0 In 1950 June 6. 12.4 13.4 13.3 10.2 12.9 July 11. 10.2 10.6 14.5 11.8 14.4 August 1. 11.2 17.0 21.4 17.9 24.5 September 1. 4.5 4.5 10.9 8.7 10.6 October 6. 6.1 7.6 10.1 6.6 10.4 Value for significant difference at 5 per cent level 2.7 in 1949, and 1.7. in 1950 Influence of legumes upon nitrification. Few methods exist at present for estimation of microbiological conditions in the soil. The two most commonly used indicators are the nitrification capacity of the soil and the production of carbon dioxide, either from the soil's own sources or from compounds added to it. These methods were used in this investigation also to indicate the influence of legumes on the microbial fertility- of the soil. First an examination of the nitrification of soil nitrogen was performed. The soil samples were bored from the middle between the plant rows, and pieces of roots or other undecomposed plant material were removed before weighing. Thus the results represent, at least in the main, the nitrification of soil nitrogen, including nitrogen of the excretions, provided such exist, and of the decomposed root matter. The nitrification rate in soils growing annual legumes and nonlegumes shows (Table 3), in general, the marked superiority of the pea and vetch soils over the oats soil. The soils growing mixed crops show no difference from the oats soil, apart from a few cases when they may be even inferior to the oats soil. The data indicate a maximum in the rate of nitrification in August. This, however, may be due partly to the laboratory temperature, at its highest during the summer. Thus, only the differences between the data of the same sampling are reliable. The residual effect of the annual plants on nitrification in the soil samples, that can be seen from Table 4, appears to be nearly similar in all plots. Some superiority in samples after pea may be found at the beginning of growing season, and the soil after vetch shows a maximum nitrification value in June. A decline in the nitrification of the soil after mixed or single vetch crop may be noted at the end of the season. 216 ARMI KAILA Nitrification of soils taken from the ley experiments indicate a considerable difference between timothy and red clover soils in favour of the latter (Table 5). Even the mixed ley crop appears to be able to produce a higher nitrification rate than the single timothy crop, except at the beginning of the growing season. The superiority of the red clover and the mixed ley last no longer than until the middle of the following summer, as can be seen from the data on the residual effect of the leys reported in Table 6. The higher nitrification rate under and some time after the legumes as compared to non-legumes noted in this investigation as well as in several earlier ones (10, 11) may be explained in various ways. The primary cause is probably connected with the amount of mobilizable organic nitrogen, which is greater in the legume plots (8), due either to the secretion of organic nitrogen compounds or to the lower carbon nitrogen ratio in the dying roots of the legumes. The accumulation of nitrate nitrogen in soil during incubation need not be associated with the abundance of nitrifying organisms, nor even with the natural nitrification capacity of the soil that depends on environmental conditions, such as aeration, acidity, and tempera- ture. Even if ammonium nitrogen is added to the soil, the increase in nitrate- nitrogen over that of untreated soil probably depends more on the energy-nitrogen relationship in the soil than on the abundance of nitrifying organisms in that parti- cular soil. Nitrification of ammonium nitrogen in the soils of this investigation (Tables 7 and 8) provided no evidence of a stimulation of the nitrate formation in legume soils compared with non-legume soils. These tests were performed in the same way as the nitrification experiments described previously, but with an addition of 200 mg of ammonium nitrogen, in the form of ammonium sulfate, per kg of soil, and the nitrification of this was calculated as the difference between the nitrate-nitrogen content of these samples after incubation and of the samples incubated without any treatment. No lime was added in these tests to neutralize the acids formed by the nitrification of ammonium sulfate. Hence, the inferiority of clover soil to timothy soil may originate from the higher acidity of the former (8). In the soils from the annual crop plots, where the differences of the pH-values are less distinct (8), no lower rate of nitrification of ammonium nitrogen could be detected in legume soils, except in the last test. Influence of legumes upon soil respiration. The influence of legumes upon soil lespiration or carbon dioxide evolution from the soil was examined under the laboratory conditions only. An experiment was performed with soil samples, collected as usual from between the plant rows, and freed of every particle of macroscopic, undecomposed plant residue. The results obtained from this experiment were taken to show whether the various plants did exert any special effect upon the rate of carbon dioxide evolution from the soil organic matter, including the more humified plant residues. 3 Table 4. Increase upon incubation in the nitrate-nitrogen content of soil samples from plots after annual legume and non-legume crops (Expressed as N03-N p.p.m. of soil dry matter). Experiment S 11. Preceding crop Date of samplings oats Mixed pea pea Mixed vetch Vetchand oats and oats May 9. 12.1 12.8 15.0 13.0 13.5 JuneB. 11.3 12.0 12.2 13.6 16.0 July 13. 18.8 17.4 15.6 18.6 17.4 August 9. 15.5 13.2 17.7 17.3 15.8 September 19. 11.7 10.2 11.6 9.1 6.8 Value required for significant difference at 5 per cent level 1.6. Table 5. Increase upon incubation in the nitrate-nitrogen content of soil samples from the ley plots. (Expressed as N03-N p.p.m. of soil dry matter.) Experiment NI. Date of Ley crop samplings Timothy Mixed crop Red clover May 12. 16.1 15.7 23.4 June 13. 6.5 9.2 12.7 July 14. 7.0 9.3 12.4 August 8. 8.1 9.7 13.8 September 12. 7.1 14.7 22.0 October 6. 6.2 10.2 16.0 Value required for significant difference at 5 per cent level 1.6. Table 6. Increase upon incubation in the nitrate-nitrogen content of soil samples from the plots after the leys. (Expressed as N03-N p.p.m. of soil dry matter.) Experiment N 11. Date of Preceding ley crop samplings Timothy Mixed crop Red clover October 27. in 1949 8.9 15.0 15.7 May 13. in 1950 10.2 13.2 13.5 June 12. 12.4 15.5 17.4 July 15. 16.7 14.9 11.7 August 2. 21.4 22.9 21.9 September 12. 9.4 11.3 11.6 Value required for significant difference at 5 per cent level 1.7. Table 7. Nitrification of ammonium sulfate added to samples of soil growing annual legume and non- legume crops. (Expressed as nitrate- nitrogen formed, p.p.m. of soil dry matter). Experiment S I. Crop Date of samplings oats Mix , ed P ea Pea Mixed vetch Vetch __ and oats and oats June 5. 157 153 147 152 175 July 11. 122 130 122 125 118 August 1. 171 165 144 156 163 Value required for significant difference at 5 per cent level 13. 217INFLUENCE OF LEGUMES ON MICROBIAL ACTIVITY IN SOIL 218 'VRML KAILA Table s Nitrification of ammonium sulfate added to samples of the soil growing ley crops. (Expressed as nitrate-nitrogen formed, p.p.m. of soil dry matter). Experiment N 1 Date of Ley crop samplings Timothy Mixed crop Red clover June 13. 92 89 83 July 14. 95 87 68 August 8. 105 101 93 Value required for significant difference at 5 per cent level 6. Table 9. Carbon dioxide evolved from the soil samples obtained from the plots of annual legume and non-legume crops. (Expressed as milligrams of CO., per 100 grams of fresh soil). Crop Period of incubation Oat. Mi*ed pea Pea Mixc 4 d V!tch Vetchand oats and oats September 25. October 3. II 7 13 9 10 October 3.-9. 7 3 4 5 4 October 9. 16. 10 !» 10 9 10 October 16. 23. 15 13 13 14 16 October 23. November 13. 13 14 13 17 14 September i_V> -November 13. 56 46 53 54 60 Table 10. Carbon dioxide evolved from the soil samples obtained from the plots of ley crops. (Expressed as milligrams of CO., per 100 grams of fresh soil). Ley cropI 'eriod oi incubation Timothy Mixed crop Red clover September 25. October 3. 29 28 28 October 3. 9. 14 14 12 October 9.—16. 11 11 9 October 16. 23. 27 24 27 October 23. November 13. 23 23 20 September 25.. November 13. 104 LOO 96 The results, reported in Tables 9 and 10, show no superiority of legumes in sti- mulating the evolution of carbon dioxide from the soil organic matter. Rather a contrary tendency is noted, particularly with red clover. The higher soil respiration rate found under and after legumes (7, 13) must therefore be attributed to the more rapid decomposition of the legume residues. Some experiments concerning the carbon dioxide production from oats, pea and vetch residues, and from the residues of red clover and timothy, all containing both stubble and roots, confirmed this opinion. INFLUENCE OF LEGUMES ON MICROBIAL ACTIVITY IN SOU 219 Sum mar v. An attempt has been made in the present investigation to elucidate the influence of growing legumes upon the microbial population and upon its activities in the soil. Annual legumes, pea and vetch were compared with oats, and red clover with timothy. At the beginning and at the end of the summer the number of bacteria, calculated through various culture media, appeared to be greater in the rhizosphere of the pea plants than under the vetch or oat plants. The rhizosphere flora of the young pea plants showed a capacity for more active nitrogen fixation than that of the young vetch or oat plants, but later the superiority of the pea plants disappeared. A higher rate of nitrification under and for some time after the legume crops compared with the corresponding non-legume crops was noted. Nitrification of added ammonium sulfate did not occur more actively under the legumes, due either to the lack of a stimulation of the nitrifying flora, or to the higher acidity in the legume soils. Compared with the non-legumes, the legumes could not be found to stimulate carbon dioxide production from the soil in any other way than through their more easily decomposable residues. Within the bounds of this investigation no special effect of legumes upon the soil flora could be established that cannot be explained on the basis of their nitrogen economy, either their utilizing soil nitrogen to a smaller degree than non-legumes, or due to composition of their roots and stubble. Under the field conditions, however, several other factors not demonstrable from this material may exist. REFERENCES (1) Berge, T. O. 1941. Determination of nitrate-nitrogen with a photoelectric colorimeter. Soil Sei. 52, p. 185—191. (2) Clark, F. E. 1949. Soil microorganisms and plant roots. Advances in Agronomy, Vol. I, p. 242—288. (3) Cramer, H. 1949. Sannolikhetskalkylen och några av dess användningar. Uppsala, 255 p. (4) Creuzberg, U. 1928. Untersuchungen über den Einfluss des Pflanzenbestandes auf das Bakterien- leben im Boden. Landw. Jahrb., 68, p. 75—115. (5) Eggi.eton, W. G. E. 1938. The influence of environmental factors on numbers of soil microorga- nisms. Soil Sei., 46, p. 351—363. (6) Graf, G. 1930. Über den Einfluss des Pflanzenwachstums auf die Bakterien im Wurzelbereich. Centrbl. Bakt. (II), 82, p. 44—69. (7) Headden, W. P. 1927, 1930. Effects of clover and alfalfa in rotation. Col. Exp. Sta. Bui. 319, 362—364. (8) Kaila, A. Influence of legumes upon soil fertility. Ann. Acad. Sei. Fennieae (In the press). (9) LöHNis, F. 1926. Effect of growing legumes upon succeeding crops. Soil Sei,. 22, p. 355—389. (10) Lyon, T. L., Bizzell, J. A. and Wilson, B. D. 1920. The formation of nitrates in a soil following the growth of red clover and of timothy. Soil Sei., 9, p. 53—64. ARMI KAILA220 (11) Newton, J. D., Wyatt, F. A., Ignatieff, \ "., and Ward, A. S. 1939, Nitrification under and after alfalfa, brome, timothy and Western rye grass. 11. Soil microbiological activity. Canad. Journ. Res. 17 C, p. 256—293. (12) Rider, P. 1948. An introduction to modern statistical methods. 4th print. New York, 220 p. (13) Ripley, P. O. 1941. The influence of crops upon those which follow. Sei. Agr., 2J, p. 522- -583. (14) Starkey, R. L. 1929. Some influence of the development of higher plants upon the microorganisms in the soil: 11. Influence of the stage of plant growth upon abundance of organisms. Soil Sei., 27, p. 355—-378 (15) —»— 1931. Some influence of the development of higher plants upon the microogranisms in the soil: IV. Influence of proximity to roots on abundance and activity of microorganisms Soil. Sei., 32, p. 367-393. ! liii Timonin, M. I. 1940. The interaction of higher plants and soil microorganisms. 1. Microbial popula- tion of rhizosphere of seedling of certain cultivated plants. Canad. Jour. Res., ISC, p. 307—317. (17) Virtanen, A. 1., Jorma, J., Linkola, H., and Linnasalmi, A. 1947. On the relation between nitrogen fixation and leghaemoglobin content of leguminous root nodules. Acta Chem. Scandinavica, 1, p. 90—111. (18) Wallach, R. H. and Lochhead, A. G. 1949. Qualitative studies of soil microorganisms: VIII. Influence of various crop plants on the nutritional groups of soil bacteria. Soil Sei., 67, p. 63—69. (1!)) —»— —»— 1950. Qualitative studies of soil microorganisms IX. Amino acid requirements of rhizosphere bacteria. Canad. Jour. Res., 28 C, p. I—6. SELOSTI - PALKOKASVIEN VAIKUTUKSESTA MAAN MIKROBITOIMINTA \ N Armi Kaila Helsingin yliopiston maanviljelyskemian laitos. Viime aikoina on entistä enemmän kiinnitetty huomiota korkeampien kasvien ja maan mikro- organismien väliseen vuorovaikutukseen ja väitetty jopa, että korkeammat kasvit säännöstelevät maan mikrobistoa voimakkaammin kuin muut ympäristötekijät (5). Palkokasvien maan kasvukuntoa paran- tavan vaikutuksenkin katsotaan johtuvan osittain siitä, että useat hyödylliset mikro-organismit viihty- vät niiden juuristossa (9). Edellä olevassa tutkimuksessa on vertailtu palkokasvien ja heinäkasvien vaikutusta maan mikro- bien määrään ja toimintaan, toisaalta hernettä ja virnaa kauraan, toisaalta puna-apilaa timoteihin. Myös sekakasvustot olivat tutkimuksen kohteena. Näytteet oli kerätty kenttäkokeista, mutta varsinai- set tutkimukset suoritettiin laboratorion olosuhteissa. Tutkimuksessa päädyttiin seuraaviin tuloksiin. Kesän alussa ja lopussa oli herneen juuriston maauute-hiivauute-, asparagiinihappo- ja ammonium- sulfaattiagarilla kasvavien bakteerien lukumäärä suurempi kuin kauran, keskikesällä ei ollut havaitta- vissa herneen paremmuutta tässä suhteessa. Virnan juuriston organismit kasvoivat verraten heikosti kai- killa näillä elatusaineilla. Asparagiinihappoagarilla kasvavien bakteerien suuri lukumäärä kesän lopussa kerätyissä herneen juuristoissa voi kytkeytyä punaisten juurinystyröiden runsaaseen esiintymiseen vielä vihreisssä herneyksilöissä, joita oli joukossa. Aerobeja vapaita typensitojia ei kasvanut typettö- INFLUENCE OF LEGUMES ON MICROBIAL ACTIVITY IN SOIL 221 mällä mannitoliagarilla mistään näytteistä, mutta typen sidontaa oli todettavissa mannitoliravinto- liuoksessa herneen organismeilla kesän alussa, kaikilla kasveilla keskikesällä, kauralla sekä hiukan myös virnalla kesän lopussa. Typen sitojina toimivat ilmeisesti Clostridium-lajit. Maan typen nitrifioituminen oli selvästi tehokkaampaa palkokasviruuduilla, jopa nurmikasvi- seoksissakin, kuin puhtaissa kaura- tai timoteikasvustoissa. Palkokasvien jälkivaikutus tuntui vain seuraavan kasvukauden alussa. Palkokasvien paremmuus perustunee helposti mobilisoituvan typel- lisen aineksen kertymiseen, koska ei voitu todeta lisätyn ammoniumsulfaatin tehokkaampaa nitrifioitu- mista palkokasviruuduilta otetuissa näytteissä vastaaviin kaura- ja timoteiruutujen näytteisiin ver- rattuna. Palkokasvien ei todettu tehostavan hiilidioksidin kehittymistä maanäytteistä, joten eräitten tutki- joiden (7, 13) tähdentämä maan hengityksen lisääntyminen palkokasvien vaikutuksesta johtunee lähinnä niiden helposti hajaantuvista kasvin jätteistä tai juurten hengityksestä. Tutkimuksen tulosten perusteella ei voitu todeta palkokasveilla olevan mitään sellaista erikois- vaikutusta maan mikrobitoimintaan, joka ei johtuisi välillisesti tai välittömästi niiden omavaraisesta typpitaloudesta. On mahdollista, että kenttäolosuhteissa on vaikuttamassa muita tekijöitä, joita ei voitu todeta tämän aineiston perusteella.