ON THE NATURE AND INCIDENCE OF GOITRE IN FINNISH DAIRY CALF POPULATION Vappu Kossila, Ulla-RiittaLehtonen, Maija-Leena Sulkinoja and Ritva Myllymaa Department ofAnimal Husbandry, University ofHelsinki Received February 9, 1970 lodine prophylaxis has been proved beneficial in preventing goitre in man and domes- tic animals. Yet there are many geographic areas in which the incidence of goitre is signi- ficantly higher if compared to some others. Apparently, either the amounts of iodine that have been used, have not been sufficiently high, or there are other regional factors, viz. goitrogens, water pollution, depletion of trace minerals from the soil as a result ofintensive cultivation, which are involved. The purpose of this study was to investigate whether, and to what extent, goitrous thyroids could be found in dairy cattle population born and raised in different areas of Finland, and to verify the prevailing histological characteristics of the respective thyroids. Since the geographic distribution of goitre in cattle seems to be positively correlated with that in man (Wiertz 1957, Stukowsky et al. 1960, Bobek & Pelczarska 1963, Brand et al. 1963), the results of this study may also be ofinterest from the human point of view. Material and methods During 25. 1.—19. 4. 1968, altogether 1124 thyroid gland samples were collected from calves and young cattle brought into the slaughteries of 16different cities located in various parts of Finland. The source of the material comprised about 60 % of the rural counties of the country. Collecdon of the samples was begun in the southern parts of Finland moving northwards. Breed, sex, carcass weight, and the exact location, in which the ani- mal was born and raised, were recorded for each case. The thyroid material, obtained from the dairy herd of the Viik Experimental Farm during iodine prophylaxis in 1960—69 (Kossila 1967, Kossila 1969), serves as a control material for this study. The said farm is located in area 17 (Table 3) within the boundaries of the city of Helsinki. https://www.c-info.fi/en/info/?token=shWeSjIV8t6gl4Tk.u1bsZT9ESKvEFb2qwTuPvw.0w-u0eBbo36ycyc1LoUXZtY3PO2YMWax5-80jdQjx4Vwz7Er37lPd3-OKGbo2D2MGSisC82nH8e4sOpTsj02osrKkv8IWOjtF1M5U-AVAfmXC3cQKVzGH9fNcaTJvD6cts2VKEKsxstkAfygBHUoDFiAWKL6gDwOl7nYBUjWMK6BtmV6wkwDfl8G95NeXDTT6Dv_yavnkCWEZlLfKaLf9B0gjz26oa9cxFtMpAobh3Bw6d6Hw6n6jeqxBg 225 The thyroid glands were weighed and handled as previously described (Kossila 1967, p. 36). Tissue preparations were made in the laboratories of the Department of Forensic Medicine, University of Helsinki and the Department of Pathology, College of Veterinary Medicine, Helsinki. The stains of Weigert van Gieson and hematoxylin-eosin were used. Several histological parameters were considered in the investigations of the morpholo- gical characteristics of the thyroids. The follicular number (FN), an index of the follicular size, was determined from ten systematically selected fields of vision with constant magnification (x125) by lanameter (Fig. 1). The number of thyroidal follicles found within the inner circle (diameter 7.5 cm) of the lanameter’s light table, were calculated by applying the following principles; a follicle of which definitely less than one half was inside the inner circle was not counted, a follicle of which definitely more than half was inside the inner circle was counted as one, two such follicles of whichabout one half was inside the inner circle were considered as one. A large numerical value of FN indicates that the follicles are small and vice versa. Epithelial cell height (Ep), an index of the intensity of the thyrotropic hormone (TSH) stimulation on the thyroid, was measured from the same areas of the tissue preparations than the FN and from such parts of the follicular epithelium which represented more or less the mean cell height of the particular area under observation. Histological activity. The thyroid preparations were first thoroughly crossexamined with a light microscope, after which they were classified, according to Fig. 1. Equipment used in the histometric measurements. Up on the left lanameter. Below on the left blood cell counter. On the right the network attached to the lanameter. 226 227 Table 1. Histological classification of the thyroids according to certain prevailing morphological charac- teristics of the gland. Activity Types of Marginal Types of follicles Amount of group epithelial cells vacuoles Size1 ) Shape 2) colloid 1 squamous rare ML(G) from round to very high oval, slight angularity often seen 1—2 from squamous to low cubic » » » » 2 from low cubic to cubic » (S)ML » » 23 mostly cubic few SML from round to oval relatively high 3 from cubic to high cubic, quite SM(L) » moderate loosely attached or free frequent cells are often seen 34 » » » about one half are from round or oval the relatively low to rest being angular fairly high or elongated 4 from cubic to high cylind- frequent SML mostly angular, ric, occasional loosely flat, elongated » attached or free cells are often seen 45 » very » mostly irregular, » plus papillary formations frequent epithelial cell out- here and there growths often invaded into follicular lumen like 4—5, but more irregular, epithelial 5 papillaries, often also » SM(L) cell outgrowths low basally cituated vacuoles strongly invaded into lumen 6 » » » » very low 1) S = small, M = medium, L = large, G = giant, ( ) less frequent type. 2) Does not concern small follicles, which usually are round. The percentages of epithelium (E %), colloid (G %), and stro- ma (S %) were estimated from each thyroid preparation in the following manner. A net prevailing morphological characteristics, into one of the activity groups described in Tab- le 1. Groups I—31 —3 were distinguished from each other mainly on the basis of the types of the epithelial cells, groups 3—5 on the basis of the types of the follicles, and groups 5—6 on the basis of the amount ofcolloid. The numerical value of each group is interpreted to indicate of the histological activity of the gland, eg. 1 indicating very low and 6 very high activity. The six micrographs in Fig. 2 (magnification x 158) demonstrate the microscopical appearance of the thyroid specimens in the six main classes described in Table 1. Fig. 2. Samples on the histological appearance of the thyroid tissue in each of the six main activity groups given in Table 1 (158 x). 228 constructed from a thin, transparent but quite firm plastic plate and from thin black sewing thread, was attached firmly to the lanameter (Fig. 1). Altogether 44 crossing points of the black thread of this net fell within the inner circle of the lanameter’s light table. Only these points were considered, when from ten fields of vision, selected as pre- viously described (Kossila 1967, p. 37), the number of crossing points that met epithelial tissue, colloid, and stroma, were registered separately for each thyroidal component with a blood cell counter (Fig. 1). In order to obtain the E %, the number of points repre- senting epithelial tissue were calculated as a percentage ofthe totalnumber ofpoints count- ed for each tissue specimen. C % and S % were obtained in similar manner. This method is a simplified modification of the histoquantitative method of Uotila & Kannas (1952), and it was developed specially for the use of the lanameter. It is very rapid (10 minutes/sample) and could be carried out with the equipment already available in the laboratory. Reliability was tested by estimating the E % from 100 thyroid specimens, in which the E % varied from 10 to 50, by the methods of this study and that of Uotila & Kannas. The average E % was found to be 25.89 and 23.16, respectively, and the coefficient of correlation between the two methods 0.7. The accuracy of the present method was con- sidered to be sufficiently high for large scale routine studies. The E % is high e.g. in poorly differentiated or iodine deficient thyroids. The absolute amount of the epithelial tissue in grams per gland (Eg) was calculated from the weight of the gland using the E %. The Eg value is raised for instance in iodine deficiency and after treatment with goitrogens. The statistical calculations were performed in the Computing Centre of the University of Helsinki. Results The present data (N = 1124) was characterized with the following range values; car- cass 7—214 kg, thyroid weight 2.1 —130.0 g, histological activity I—6,1 —6, FN 9.0—84.7, Ep 2.2—22.5, Eg 0.44—98.15, E % 14.4—88.2, G % 4.1—85.1, S% 0.9—14.6. Respective mean values as well as the distribution of the material according to breed and sex are given in Table 3. Correlation study. In order to find out whether, and to what degree: a) breed, sex, and weight of the carcass affected the weight and other characteristics of the thyroid, and b) the thyroid weight was correlated with various other characteristics of the gland, a correlation matrix (Table 2) was calculated for the entire data. As results in Table 2 show, breed and sex were not appreciably correlated with the various thyroid characteristics. On the other hand, carcass weight correlated fairly closely with the thyroid weight (.44), histological activity (.47), and Ep. (.36), and to a lesser extent also with the FN (—.17), Eg (.23), S % (.22), and C % (—.11) but hardly at all with the E % (.05). These results indicate that of the various thyroid characteristics, the E % was most ofall independent of the variations in the carcass weight. Thyroid weight correlated very closely with Eg (.86) and fairly closely with histological activity (.37), Ea (.40), E % (.39), and C % (—.40) and to a lesser extent also with FN (—.10) and S % (.20). These results indicate that heavy thyroids in this data tended to be Table 2. Simple correlations between breed, sex, carcass weight, and thyroid characteristics (N = 1124). Carcass Thyroid Hist. Variable Breed Sex wt. wt. activ. FN Ejjl Eg E % G % S % Breed i .07 —.04 —.06 .08 —.03 .02 —.03 .01 —.03 !ÖÖ Sex 1 —.12 —.07 .02 .02 .02 —.03 —.Ol .01 .05 Carcass wt. 1 .44 .47 —.17 .36 .23 .05 —.ll .22 Thyroid wt. 1 .37 —.lO .40 .86 .39 —.40 .20 Hist, activity 1 .25 .76 .38 .45 —.52 .43 FN 1 .16 .01 .24 —.24 .14 E(x 1 .46 .50 —.54 .36 Eg 1 .65 —.64 .24 E % 1 —.98 .44 C % 1 —.59 S% 1 Significance of the correlations has been tested with a formula: t = rj/i^—(ref. Croxton & Cowden 1955). According to this test, all coefficients of correlation that were ±-15 or closer are highly significant (P<0.001) Table 3. Numbers of breed and sex, and the weight of the carcass, characteristics of the thyroid, and consumption of iodized mineral salt mixtures on the average in various Agricultural Societies. Agricultural Societies Number ofcases t A v A \Hist.act. 2 ) jJ3 ) No *) Name Ay Fc 3*l % I*9B o bo 4_g i-S 6 & a 9 $ All 5i $ % FN E|i Eg E% C%S% 111l 9 Kymenlaakso & South- 36 36 6 7 85Karelia 17 District of Nyland & 39 31 7 77Nyland’s Swedish 3 Tavastland-£ataiunfa 4 1 16 15 36 2 District ofTavastland 7 9 3 9 28 10 The Far North & Lapland 9 1 36 24 70 7 Central-Finland 9 8 7 12 369 8 7 12 36 5 Kajaani 16 5 29 23 73 4 East-Tavastland 22 18 10 10 69 8 Kuopio 28 14 27 27 96 12 District of Mikkeli 9 7 14 15 45 13 Oulu 29 19 27 20 95 15 North-Karelia 14 8 35 24 81 16 Satakunta 28 25 29 22 104 1 South- & Middle-Bothnia & Osterbotten’s Swedish 85 47 27 26 185 18 Finland-Proper & 17.1 8.47 2.3 13 26.1 4.5 2.24 24.6 72.3 3.1 8.2 41 24.7 12.82 2.9 25 24.8 6.0 5.41 29.9 67.0 3.1 16.9 45 36.6 9.22 2.4 8 22.0 5.0 2.23 23.6 73.7 2.7 0.0 44 39.9 14.10 3.1 29 20.9 5.2 6.43 29.0 67.4 3.6 14.3 38 44.0 11.10 2.4 10 18.7 4.2 2.66 23.7 73.5 2.8 5.7 46 54.9 11.09 3.1 17 23.7 4.9 2.63 24.9 70.6 4.5 5.6 35 60.6 11.85 3.5 29 22.3 5.4 3.73 28.1 68.2 3.7 5.5 47 64.8 17.22 3.0 18 20.8 5.0 5.70 27.3 69.6 3.1 7.2 42 64.9 11.91 2.9 17 22.5 5.2 3.00 24.9 71.1 4.0 2.1 39 71.0 14.59 3.2 31 23.1 6.7 4.26 26.5 68.6 4.9 11.1 38 71.2 12.62 3.0 20 23.3 4.4 3.61 27.4 68.7 3.9 7.4 47 80.6 15.40 3.6 37 21.7 4.5 5.35 27.8 67.3 4.9 8.6 33 83.7 11.61 3.0 17 19.3 5.5 2.99 24.7 71.5 3.8 2.9 40 95.5 14.42 3.1 13 21.2 5.1 3.73 25.8 70.7 3.5 2.2 40 Finska Hushällning 40 10 3 53 124.0 17.54 2.9 21 18.2 4.3 4.00 23.4 72.9 3.6 1.9 44 All 375 239 276 234 1124 66.6 12.95 3.0 20 21.9 5.0 3.77 26.1 70.2 3.7 6.1 41 1) Geographic location ofeach Society is shown in Fig. 3. 2) X = mean, % 4—6 =percent of cases classified as 4< 3) lodized mineral salt mixtures 229 230 Table 4. Elimination of the effect of the animal’s size (carcass weight) on some of the thyroid characteristics. Histological activityThyroid weight Eg Area Y 1) D y 2 ) Control D c 3) Y 1) Dy 2) Control D c s) Yl ) D y s) No. % % % % % 9 8.64 —1.97 7.5212.63 2.36 17 9.3037.85 8.2056.34 2.58 3 10.34 —10.83 9.200.22 2.92 2 10.6232.76 9.5048.42 3.01 10 10.981.09 9.8013.27 3.13 7 11.93 —7.04 10.654.13 3.43 5 12.43 —4.67 10.908.72 3.60 4 12.7934.60 11.0356.12 3.72 8 12.80 —6.95 11.047.88 3.72 12 13.339.45 11.2529.69 3.89 13 13.35 —5.47 11.3311.39 3.90 15 14.178.68 11.6032.76 4.16 16 14.44 —19.60 11.76 —1.28 4.25 1 15.47 —6.79 12.2018.20 4.59 18 17.95 —2.51 13.3031.88 5.39 —5.09 1.4257.75 2.30.0 109.691.45 273.102.4 12.5 —23.63 1.5246.71 2.6 —7.7 113.621.55 314.842.6 19.2 —15.02 1.5670.51 2.7 —ll.l —23.32 1.6361.35 2.810.7 3.611.70 119.412.9 20.7 53.231.74 227.593.0 0.0 —19.36 1.7571.43 3.0 —3.3 9.511.82 134.073.0 6.7 —7.43 1.8496.20 3.1 —3.2 28.611.95 173.363.2 12.5 —29.65 2.0049.50 3.2 —6.3 —18.74 2.1573.49 3.4 —B.B —25.79 2.5060.00 3.8 —23.7 1) Y = theoretical value obtained from the corresponding estimating equation presented in the text 2) D y = deviation of the true mean value of each area (Table 3) from Y 3) D c = » » » » » » » » from the control hyperepithelial, hyperactive, and poor in colloid. Histological activity correlated closely with Ep (.76), fairly closely with Eg (.38), E % (.45), C % (—.52), and S % (.43), and to a lesser extent with FN (.25); FN correlated to a lesser extent with Ep. (.16), E % (.24), C % (—.24), and S % (.14). Ep correlated fairly closely with Eg (.46), E % (.50), C % (—.54), and S % (.36); Eg correlated quite closely with E % (.65) and C % (—.64), and C % with S % (—.59). Geographic distribution of goitre. The present data was first divided into 15 groups according to the Agricultural Society from whose area the samples were taken (Table 3). The mean carcass weight and the means of the various thyroid characteristics were calculated for the 15 groups. The groups were then arranged according to mean carcass weight (Table 3). The observation of the results is somewhat simplified in this way, since the weight and some other characteristics of the thyroid were found to be significantly influenced by the size of the animal. Although it was previously noted thatbreed and sex had very little effect on the characteristics of the thyroid (Table 2), the number of Ayrshires (Ay), Finncattle (Fc), and ?in each group have, nevertheless, been given in Table 3. Thyroid weight. The mean thyroid weights obtained for each area are not di- rectly comparable with each other because of the carcass weight being an interfering factor. Its effect was eliminated by means of calculating first the theoretical thyroid weight (Y) from the equation obtained for the entire data (N = 1124); Y = 7.147 + 0.087 X, in which Y = thyroid weight in g and X = carcass weight in kg, and then estimating the deviation of the true mean value in percent from the theoretical one (D y). The results obtained this way have been presented in Table 4. 231 Another method which also was used, was the estimation of the deviations of the true mean values from the control values (D c ) (Table 4). This method has been preferred particularly since it became evident that the rate of increase of the thyroid weight in the controls was somewhat more rapid up to 100kg body weight (corresponds to carcass weight of 50 kg, if slaughter percentage is assumed to be 50) than thereafter (Kossila 1967, p. 78). The geographical distribution of goitre in Finland, judged by the deviation of the mean thyroid weight in each area from the control, is demonstrated in Fig. 3. It shows that the thyroid weight has been highest in areas 2,4, and 17, somewhat raised in areas 12, 15, and 18, only slightly raised in areas 1,5, 8,9, 10, and 13, but similar to control herd in areas 3,7, and 16. E %. The thyroidal E % was found not to be influenced by the carcass weight (Table 2). Therefore, the regional differences can be observed as such (Fig. 4). The E % was highest in areas 2 and 17 and lowest in areas 3,7, 8,9, 16, and 18. Even in the latter areas, the E % has been somewhat higher than in the control herd, in which the young animals had an E % below 20 on an average (Kossila 1967 p. 77). E g. The thyroidal Eg value was found to be somewhat influenced by the carcass weight (Table 2). The theoretical Eg values obtained separately for each area from the regression equation based on the present data: Y = 1.878 + 0.028 X, in which Y = Eg and X = carcass weight in kg, as well as the Eg values representing the control herd are given in Table 4. The theoretical Eg values obtained from the above equation are higher compared to control values (Table 4). The deviationof the Eg as a percent from the control (Fig. 5) was found to be highest in areas 2 and 17, markedly raised in areas 4 and 15 and somewhat less raised in areas 5, 12, and 13. Yet in the remaining areas, the Eg was notably higher (from 45—75 %) compared to the control values. Histological activity ofthe thyroid was found to be influenced by the car- cass weight (Table 2). The regression equation obtained from the entire data had the form; Y = 2.025 -j- 0.014 X, in which Y = activity class (Table 1,) and X = carcass weight in kg. The deviations of the true mean activity values from the theoretical values, obtained from the above equation, are given in Table 4. They indicate, among others, that histo- logical activity was high in areas 2 and 5, but low in areas 10 and 18. The present data included about 20 % of cases classified histologically as 4 or higher, while no such thyroids were found in the controls. In this study activity classes 5 and 6 considered histologically definitely as abnormal. Regional incidence of abnormal thyroids is demonstrated in Fig. 6. High incidence (10 %<) was found in areas 2, 12, and 17, relatively high (7.2—8.6 %) in areas 4,9, 13, and 15. In areas 5,7, and 10, incidence was 5.5—5.7 %. In areas 1,3, 8, 16, and 18it was rather low (<2.9 %). Other thyroid characteristics are considered only briefly in this con- nection. The highest mean FN values were found in areas 9 and 17, these data comprising very young animals, and the lowest values in areas 10, 16, and 18, the two latter data com- prising rather large animals (Table 3). The mean E(j. value was high in areas 12 and 17 but low in areas 10 and 18. Areal trends in C % were opposite to those of E %. The mean S % was highest in areas 12 and 15 but lowest in areas 3 and 10. Consumption of iodized mineral salt mixtures in kg/cow/year in the area of each Agricultural Society (Table 3) was calculated from the data made available specially for this study by various feed factories. In this way it was possible to trace the regional distribution of 83 % of the total of 43189 tons sold in Finland in 1967. lodine in these mixtures was in the form of either potassium iodide (0.004—0.006 %) or copper iodide (0.010 %). Average consumption in the whole country was 40.8 kg/cow/year, which is only about 1/3 of the amount used in the control herd. As values in Table 3 indicate, in the areas 5, 10, and 13, which are located in North- Finland, and in which the indoor feeding period is relatively long, the consumption has been relatively high, while the incidence of goitre has been relatively low. On the other hand, in area 17, which is located in South-Finland, and in which the goitre incidence was found to be high, the consumption rate was also relatively high. Relatively low consumption levels in turn, were found in one nongoitrous area, 7, and in the goitrous areas 2, 12, and 15. There appeared to be a certain tendency for the percentage of the cases classified histologically as 4—6, and particularly the S %, to decrease with increasing consump- tion of the iodized mineral salt mixtures (Table 3). Fig. 3. Deviation of the mean thyroid weight in in percent from the control in each area. Area numbers are found in Table 3. Fig. 4. Mean percentage of epithelial tissue of the thyroid (E %) in each area. 232 Discussion Incidence of goitre was found to be highest in areas 2,4, 12, 15, and 17, according to the extent of the rises in the weight of the thyroid, the E %, Eg, and the percentage of histologically abnormal glands (Fig. 3—6). The percentage of histologically abnormal glands was relatively high (7.4—8.2 %) in areas 9 and 13, and the histological activity, E(x, and E % raised in area 5, the thyroid weights being quite normal respectively. The thyroid weight was found to be somewhat raised in areas 1 and 18, and the percentage of histologically abnormal glands fairly high (5.6—5.7) in areas 7 and 10, the other signs of goitre being either weak or missing in these areas. Relatively few histologically abnormal glands were found (2.1 —2.9 %) in areas 8 and 16, while other signs of goitre were not observable. No signs of goitre were found in area 3 with the methods used in the present study. In this study, only the thyroids, classified histologically into activity groups 5—6, were considered as abnormal. However, in addition to these, a few cases in the activity groups I—2 exhibited degenerative transformations, such a condition is rare in young animals, however. Fig. 5. Deviation of mean epithelial tissue content of the thyroid (Eg) in percent from control in each area. Fig. 6. Percentage of histologically abnormal thyroids in each area. 234 Unfortunately thepresent data lacks material from areas located near the cities lisalmi, Savonlinna, and Hämeenlinna and from the area eastwards from Joensuu, the three latter being rated as human goitre districts. Furthermore, it would have been better from the point of view of this study ifthe animals had been more uniform in age. The results of this study agree with those ofAndersson (1960), whofound a higher E % in the thyroids of calves derived from Helsinki, Lahti, and Joensuu (correspond to areas 17, 2+ 4, and 15 of this study) compared to those derived from Pori, Seinäjoki and Kristiina (correspond to areas 1 and 3 of this study). According to general opinion, goitre is rare in sea coast areas. However, Wiertz (1957) found goitre even in cattle kept on the seacoast area of the Netherlands. In this study, goitre was found particularly in area 17, which is located on the coast of the Gulf ofFinland. According to several studies made in other countries, geographic distribution ofgoitre in cattle appears to be parallel with that in man. Similar conclusions can be drawn, if the results of the present study are compared with those obtained earlier in the investigations on human goitre in Finland (ref. Nordman 1968, p. 12—15). Furthermore, the incidence of goitre in Finland seems to be related to the iodine content in the diet. Consumption of iodized mineral salt mixtures by cattle varied somewhat in different Agricultural Societies, but not enough to explain satisfactorily the differences noted in the incidence of goitre. In the control herd, in which no histologically abnormal thyroids were found in young cattle during 1960—69, the consumption of iodized mineral salt mixtures was three times as high as the respective value representing the whole country in 1967. It should be noted that the control herd is located in a goitrous area, 17 (Fig. 3), and that goitre was also found in this herd before the introduction ofiodine prophylaxis (Kossila 1967). Judging by the results of this study it can be assumed that, either the distribution of the use of iodized mineral salt mixtures has not been wide enough, or that the amounts of iodine in these mixtures have not been high enough to prevent goitre in certain areas. Therefore, it is recommended that consumption ofsupplemental iodine should be increased considerably in areas 2,4, 12, 15, and 17, and moderately in areas 1,5, 7,9, 10, 13, and 18. On the other hand, in areas 3,8, and 16, the intake of iodine by cattle has apparently been almost sufficient for preventing signs of goitre. Furthermore, iodine requirements are likely to rise with increasing efficiency in animal production, particularly during lacta- tion, and also dry, pregnant cows require sufficient amounts of iodine to prevent goitre in the developing fetus. Requirements for supplemental iodine depend on the natural content of iodine as well as on the amount of various goitrogenic factors in the feedstuffs and drinking water. Only after the amounts of supplemental iodine in the diet of dairy cattle have been markedly increased from the level used in 1967, is it possible to draw conclusions as to whether there are such areas in which goitre cannotbe prevented by means of iodine prophylaxis. Summary In 1968, thyroid glands were obtained from young dairy animals born and raised in the areas of various Agricultural Societies of Finland. Breed, sex and carcass weight were recorded for each case. The thyroids were weighedand preparations made for the histological examination. The relative proportions of epithelium (E %), colloid (C %) and stroma (S %), and the height of the epithelial cells (Ep,), were estimated with histometric methods, 235 and the absolute amount of epithelial tissue in grams per gland (Eg) was calculated. The relative size of the follicles (FN) was also determined. The glands were classified, on the basis of prevailing histological characteristics, into activity groups indicated by numbers from 1 (low) to 6 (high). The glands in groups 5 and 6 were considered histologically as abnormal. The data included both Ayrshires (375