Maataloustieteellinen A ikakauskirja Vol. 57: 75—84, 1985 The effect of supplementation of grass silage with rapeseed meal or Gasol-treated barley on the performance of growing cattle PEKKA HUHTANEN, ESKO POUTIAINEN' and TIMO MIKKOLA2 Department of Animal Husbandry, University of Helsinki, SF-00710 Helsinki Abstract. Grass silage was offered ad libitum to 16 Ayrshire cattle in a 2 x 2 factorial experiment lasting 196 days. The silage was supplemented with barley preserved with 0.85 % of propionic acid (PAB) or 1.28 % Gasol solution (GB) 45 g DM/kg W 0 75 . Four of the ani- mals in PAB and GB groups received no protein supplement (NPS) and four of the animals in both groups received extracted rapeseed meal as protein supplement(RSM). RSM was fed according to UDP (undegradable feed protein) requirements. The average age of the animals at the beginning of the experiment was 86 days and the average liveweight 106 kg. The average daily gains were 1066 and 1070 g/d for PAB and GB groups and 1012 and 1124 g/d (P < 0.01) for NPS and RSM groups. In feed intake and feed conversion there were no significant differences between the groups. Supplementation of silage barley diet with RSM increased the digestibility of organic matter from 71.1 to 73.5 % (P < 0.05), of crude protein from 62.8 to 67.4 % (P < 0.05) and of crude fibre from 57.2 to 60.2 % (P < 0.05). Both barleys were well preserved and there was no deterioration during the storage. The degradation rates of DM, crude protein and starch determined by nylon bag method were lower in GB than in PAB. Crude protein disappearances in 9 hours were 46.6 % for GB and 76.4 % for PAB. The utilization of absorbed protein calculated by factorial method averaged 0.566 ± 0.01 in the four different groups. Plasma urea N level was higher (P < 0.05) in the RSM than in the NPS group. The pro- portion of acetic acid in the rumen VFA was lower (P < 0.05) and that of propionic acid higher (P < 0.01) in the RSM than in the NPS group. PAB resulted in a higher (P < 0.05) proportion of propionic acid in rumen VFA than did GB, Introduction New protein evaluation systems recognize that the amino acids available to ruminants will be supplied partly by microbial protein synthesis in the rumen and partly from di- etary protein, that is undegradable in the Present adress: Agricultural Research Centre, SF-31600 Jokioinen. 2 Farmos Group, Agricultural Division, Tengströminkatu 6. SF-20360 Turku. Index words: rapeseed meal, protected barley, protein utilization, growing cattle rumen. According to the ARC system (Anon 1980), growing cattle of more than 250 kg 75 JOURNAL OF AGRICULTURAL SCIENCE IN FINLAND https://www.c-info.fi/en/info/?token=WrKr10vAsg_LNiwj.tWeQR5F3_x4OEvYHuId4JA.zEchySsjiARe6hoNxq6q0RetjH_eLZn7Q7JTXQezuf30vR-b2Mg11gzGbrblcQo2TWgq4aOUX1xlfQYB1O2OQFCFVlNsXYIs38JaOp6lvVAxX2AldaZrh8VEsfNdGLY8DCjXzg6-il7Eh7Thwj7IuYaUxYTvVgjIs4P1dU4R5bZGI_EtpPdp-tXFSfZ8RF7la5MihmkuI6NXktnpExM can meet their protein requirements from microbial synthesis. More recent informa- tion (ORSKOV and McLeod 1982) suggests that endogenous N losses may be greater than previously thought and protein reguire- ments may need to be revised upwards. The crude protein of grass silage is rapidly degraded in the rumen, especially when the NPN content of the silage is high (Setälä 1983 a), and the supplementation with un- degradable protein may therefore be advan- tageous for animals with high protein require- ments. In many experiments supplementa- tion of grass silage with fish meal (FM) (Garstang et al. 1979, Kirby and Chalmers 1982, Kirby et al. 1983 a, Kay and Scott 1984) or with soyabean meal SBM (Kirby et al. 1983 b, Waterhouse et al. 1983) has in- creased the liveweight gain of growing cattle. The responses to liveweight gain are partly due to increased silage DM intake and partly to an increase in undegraded feed protein. Values for efficiency of microbial protein synthesis in animals given silage diets are lower than in animals given hay (Thomas 1982). In addition the proportion of methi- onine of total amino acids in duodenal di- gesta is lower on silage than on hay diets (Thomas 1982). Methionine is frequently the first limiting amino acid in ruminants. In Finland beef cattle feeding is often ba- sed on silage ad libitum and 2—4 kg of barley/d. Protein supplements are seldom used in grass silage based feeding. On such a diet the amount of UDP is very low and the protein flow into the duodenum depends mainly on microbial protein synthesis. One possible method to increase dietary protein flow into the duodenum is to protect it against microbial degradation in the rumen. According to a review by Kaufmann and Topping (1982), protected protein tends to improve weight gain and feed conversion. The present experiment was designed to evaluate the effect of rapeseed meal, the most important protein supplement produc- ed in Finland, and Gasol-treated barley as UDP sources for growing cattle on silage based diet. Material and methods Animals and feeding A feeding trial of 2 X 2 factorial design was carried out with 12 bulls and 4 heifers of Finnish Ayrshire breed. The animals were taken in four blocks into the experiment, at the average weight of 106 kg and average age of 86 d. The animals were divided into four similar groups with respect to sex, age and liveweight. The four treatments consisted of two barleys, each either supplemented with rapeseed meal (RSM) or given without sup- plements (NFS). The barleys were preserved at a moisture content of 20—22 % with addi- tion of 0.85 % of propionic acid (PAB) or 1.28 % of Gasol solution (GB), which is a mixture of organic acids and formalin. The experiment lasted 196 days after which the bulls were slaughtered. The animals were weighed at the beginning and end of the ex- periment on two consecutive days and during the experiment every second week. Unwilted grass silage, preserved with AIV II solution (80 % formic acid, 2 °7o ortho- phosphoric acid), was offered ad libitum in quantities that were s—lo5—10 % in excess of the previous day’s consumption. In addition to the silage, 45 g DM/kg W 0 75 of rolled bar- ley was offered. In the RSM group the ani- mals received extracted RSM according to calculated UDP requirements (ARC, Anon 1980). If there was no calculated UDP require- ment the amount of RSM was then 0.2 kg/d. A commercial mineral mixture was provided to the amount of 100 g/d and a vitamin mix- ture every week according to the require- ments. Barley, RSM and mineral mixture were mixed prior to feeding. The animals were fed individually twice daily. Feed re- fusals were weighed every day. Sampling and analyses The feeds were sampled once a week and 76 pooled into one sample for two weeks for si- lage and barleys and into one sample for four weeks for RSM. DM determination was made of every sample and feed analyses of pooled samples. DM content was determined at 103°C and samples for feed analyses were dried in vacuum at 50°C. DM content of si- lage was corrected and pH, lactic acid, sug- ars, ammonia N and VFA were analyzed as described by Huhtanen (1984 a). Feed analyses were made according to standard methods. Degradabilities in the rumen of crude pro- tein and DM of all experimental feeds and of starch of barleys were determined by nylon bag method (Setälä 1983 b). The feeds were incubated in the rumen of sheep receiving the same feeds in the same proportion as the ani- mals in the feeding trial. The feeds for in sac- co determinations were pooled into one sample for four weeks. The starch content of barleys and nylon bag residues was deter- mined by the method of Salo and Salmi (1968). Rumen samples were taken from the bulls 10 weeks after the start of the experiment with a 2 X 60 mm needle through the rumen wall before, and 2.5 and 5 hours after the morning feeding. pH was measured immedi- ately; ammonia N was measured by the meth- od of McCullough (1967) and VFA from deep frozen supernatant by the method of Huida (1973). Blood samples were taken from Vena jugularis at the beginning of the experiment and every eight weeks thereafter before the morning feeding. The samples were treated and analyzed as described by Huhtanen (1984 b). In vivo digestibility of the rations was de- termined by indicator method with chromic oxide as marker. Five grams of chromic oxide paper was fed directly into the oesophagus twice daily for 12 days. Grab samples of faeces were obtained from the rectum during the last five days, twice daily. Faeces were dried and analyzed by standard methods as feed samples. Chromic oxide was determined by the method of Petry and Rapp (1971). Calculations Rumen degradable protein (RDP), UDP and organic matter fermented in the rumen (OMF) were calculated using degradabilities obtained from nylon bag studies. OMF de- termined by nylon bag method was assumed to be the true digestibility in the rumen. Ap- parently fermented organic matter in the rumen (OMFA ) was calculated assuming a value of 20 g N/kg truly digested OM for the efficiency of microbial synthesis in the rumen and assuming the N content of microbial OM to be 10 %. The UDP requirement was calculated according to ARC (Anon 1980). The efficiency of microbial synthesis has been shown to be lower on silage diets than on hay diets (Armstrong 1980, Thomas 1982). The value used for the efficiency of microbial synthesis was 25 g N/kg OMFA proposed by Thomas and Chamberlain (1982) for silage diets, which is 5 g lower than proposed in the ARC system for all diets. The differences between the groups were tested with three-way analyses of variance with interaction between the type of barley and protein level (Steel and Torrie 1960). Results and discussion Feed composition, feed intake and liveweight gain The mean chemical composition, calcu- lated energy and DCP values and degradabil- ities are given in Table 1. There were large differences in nutritional value of the silage (OM in vitro digestibility 60.4—71.6 %) and in quality of the silage (ammonia 3.6—13.3 % of total N). The barleys were well preserved and the additives caused no differences in the quality. The proportion of water soluble N of total N was higher in PAB than GB (16.9 vs. 7.4). GB had a clearly slower degradation rate of crude protein in the rumen, and also the degradation rate of DM and starch were slightly slower (Fig. 1). 77 Table 1. Chemical composition and feeding values of experimental feeds. Grass ll Propionic-acid Gasol treated Rapeseed silage treated barley barley meal Dry matter, % 22.5 81.3 79.3 88.8 In dry matter, % Ash 7.4 3.1 3.0 8.1 Crude protein 16.3 12.9 12.4 37.9 Ether extract 6.0 2.4 2.4 2.6 Crude fibre 33.2 6.6 7.0 14.2 NFE 37.1 75.0 75.2 37.3 FU/kg DM 0.69 1.08 1.08 0.91 DCP g/kg DM 107 90 87 314 ME MJ/kg DM 9.8 12.6 12.6 11.0 Degradability, % Organic matter 58.1 77.3 67.2 45.3 Crude protein 84.4 77.5 47.2 54.9 FU = feed unit. 11 pH 3.97, lactic acid 5.7 Vo, sugars 5.5 Vo, acetic acid 1.9 Vo, propionic acid 0.1 Vo, butyric acid 0.2 Vo of DM, ammonia N 6.6 Vo of total N. Table 2. The average feed intake (kg DM/d) and nutrient consumption. Barley Protein SEM PAB GB NPS RSM Grass silage 2.29 2.44 2.40 2.34 0.07 Barley 2.14 1.98 1.97 2.15 0.11 Rapeseed meal 0.12 0.09 0.21 0.01 Total DM 4.55 4.51 4.37 4.69 0.14 DMg/kgW" 83.5 82.1 81.6 84.0 1.2 FU/d 3.99 3.90 3.78 4.11 0.13 ME MJ/d 50.8 49.9 48.4 52.4 1.6 DCP g/d 486 474 442' 518" 13 Means with different letters significantly different: c.d (P < 0,01). Feed intake and nutrient consumption data are given in Table 2. RSM had no effect on silage DM intake, which is in dis- agreement with the results obtained with other protein supplements, e.g. with SBM (Waterhouse et al. 1983, Kirby et al. 1983 b), with FM (Kay and King 1984, Kirby et al. 1983 a, 1983 b, Gill and England 1983) and with groundnut meal (Gill and England 1984). Chalmers et al. (1983) did not find any differences between EM and SBM in the effect on silage DM intake. The response to supplementary protein may depend on the fermentation quality of the silage. Thomas et al. (1980) observed that the intake with FM supplement increased more when the lac- tic acid content of the silage was higher. In the present study the following correlations between the silage parameters and silage DM intake/kg W 0 75 were obtained: DM content 0.224 (P < 0.01), crude protein 0.388 Fig. I. The effect of Gasol treatment on degradation rate of barley DM, crude protein and starch. 78 Table 3. Animal performance and carcass data. Barley Protein : SEM PAB GB NPS RSM — Number of animals 8 8 8 8 Initial weight, kg 105.1 107.0 106.4 105.8 4.3 Final weight, kg 314.1 316.8 304.8 326.1 9.3 Daily gain, kg/d 1.066 1.070 1.012' 1.124d 0.025 Feed conversion, FU/kg gain 3.77 3.64 3.75 3.66 0.13 Slaughter weight l », kg 326.5 330.3 322.8 334.0 9.5 Carcass weight, kg 163.7 162.8 158.2 168.3 5.2 Dressing % 50.1 49.3 49.0 50.5 0.7 Only bulls were slaughtered. Means with different letters significantly different c,d (P < 0.01) (P < 0.001), crude fibre —0.463 (P < 0.001), OM in vitro digestibility 0.354 (P < 0.001), sugars 0.306 (P < 0.001), lac- tic acid —0.030 (NS), butyric acid —0.295 (P < 0.001), ammonia N ®/o of total N —0.359 (P < 0.001) and soluble N % of total N 0.038 (NS). between 80 and 240 kg liveweight there were no differences between RSM and SBM. Protecting barley with Gasol had no effect on liveweight gain, which agrees with the re- sults of Edwards et al. (1984) for barley treated in the same way. Kaufmann and Luffing (1982), on the other hand, found an average higher liveweight gain with pro- tected protein than with untreated protein. The amino acid composition of barley pro- tein, however, is poor compared with micro- bial protein or tissue protein. Alaviuhkola and Näsi (1984) found lower lysine content in Gasol-treated barley than in dried barley. Thornton et al. (1977) reported increasing nonammoniaN flow into the duodenumwith increasing formaldehyde content in ensiled corn, but N retention was not increased and N excretion in urine was increased with form- aldehyde treatment. Davis and Faichney (1973) reported lower liveweight gain with formaldehyde treated barley than in untreated barley. In the present study there was no in- teraction between protein supplement and barley treatment in feed intake, daily gain or feed conversion rate. In carcass characteris- tics there were no significant differences between the treatments. Dressing % tended to be higher in the RSM than in the NPS group. RSM supplementation increased signifi- cantly (P < 0.01) the liveweight gain of the animals (Table 3). The difference in daily gain between NPS and RSM was higher in the first part of the experiment (0.95 kg vs. 1.10kg on days I—9B1 —98 and 1.08 vs. 1.14 kg on days 99—196), suggesting that at that time the amino acid flow into the duodenum was the growth limiting factor. Finnish DCP re- quirements (Salo et ai. 1982) were not met in the NPS group during the first month of the experiment. RSM had no significant ef- fect on feed conversion rate. The effect of RSM on daily gain agrees with the effect of protein supplementation on silage based diets (Garstang et al. 1979, Kirby et al. 1983 a, 1983 b, Waterhouse et al. 1983, Kay and Scott 1984). The response to FM has often been higher than to SBM (Kirby and Chalmers 1982, Kirby et al. 1983 a). Also on ad libitum concentrate feeding the response to FM has been better than to SBM, especially in younger bulls (Lindberg and Olsson 1983). Olsson (1978) reported re- duced performance in calves fed a ration containing 13 % of high glucosinolate RSM compared with calves fed SBM, but for cattle RSM supplementation significantly im- proved the digestibility of DM, OM, crude protein and crude fibre (Table 4). The same effect was found earlier for supplementary 79 Table 4. Digestibility of the different rations (%). Barley Protein SEM PAB GB NPS RSM Dry matter 71.0 69.7 69.2* 71.5b 0.67 Organic matter 72.9 71.7 71.1" 73.5" 0.64 Crude protein 66.6 63.6 62.8a 67.4" 1.07 Ether extract 69.1 66.7 66.4 69.4 0.98 Crude fibre 58.8 58.6 57.2» 60.2 b 0.95 NFE 79.1 78.0 77.7 79.2 0.59 Means with different letters were significantly different: a,b (P < 0.05) Table 5. Nitrogen utilization of the animals. Barley Protein PAB GB NPS RSM Crude protein, g/d 707.0 692.8 653.0 746.3 RDP, g/d 563.5 482.6 497.5 548.2 Microbial protein, g/d 364.5 332.0 337.3 360.3 UDP, g/d 143.5 210.2 155.6 198.1 Total, g/d 508.0 542.2 492.9 558.4 Absorbed protein, g/d 355.6 379.5 345.0 390.9 TP'Vabsorbed protein 0.585 0.548 0.581 0.551 ') Tissue protein, calculated according to ARC (Anon. 1980) FM (Bax and Offer 1982, Gill and Eng- land 1983). Gill and England did not find significant differences between FM and more degradable groundnut meal in the effect on silage digestibility. The reason for the posi- tive response to supplemental protein may be increased digestion in the rumen. Lindberg (1984) found the digestibility of silage in the rumen determined by nylon bag method to increase from 54 % to 64 % when 2.6 kg bar- ley DM was replaced with SBM in the diet of dairy cow. In silage the major proportion of the protein is degraded in the silo and this may result in a shortage of amino acids and/or peptides, which are believed to be im- portant growth factors for rumen microbes. In the present study Gasol treatment tended to decrease the apparent digestibility of crude protein, which effect is often found when high levels of formaldehyde are used (Kaufmann and Luffing (1982). animals did not meet their UDP require- ments because they did not eat all the feed given. Calculated utilization of absorbed protein for conversion into tissue protein was on average 0.566 ± 0.010 for different diets (Table 5), which is lower than proposed by ARC (Anon. 1980). Satter and Roffler (1975) and Verite et al. (1979) suggested the utilization of absorbed amino N for growth to be 0.60. In the present study the calculated efficiency for protein utilization decreased with increasing liveweight. The following linear regression was calculated between pro- tein utilization and liveweight: y = 1.060 0.0022 X, where y = protein utilization and x = liveweight (kg) Low efficiency for protein utilization in the later part of the experiment suggests that absorbed amino acids were no longer a growth limiting factor and the effect of RSM after 230—250 kg of liveweight was based mainly on increased energy intake. On the other hand, Kirby et al. (1983 a) reported Nitrogen utilization At the beginning of the experiment the 80 Table 6. pH, ammonia N and VFA in rumen fluid. The values are averages of different sampling times. Barley Protein SEM PAB GB NPS RSM pH 6.64 6.70 6.74 6.60 0.09 Ammonia N, mmol/1 6.02 5.33 4.95 6.38 0.93 Tola! VFA, mmol/l 98.1 98.0 94.7 101.2 4.2 Molar per cent of Acetic acid 66.3 67.4 67.7» 66.0" 0.5 Propionic acid 20.7* 19.6" 19.4- 20.9d 0.3 Butyric acid 9.6 9.4 9.5 9.5 0.4 Isovaleric acid 1.9 1.6 1.8 1.7 0.1 Valeric acid 1.4 1.3 1.3 1.3 0.1 Means with different letters were significantly different: a,b (P < 0.05), c,d (P < 0.01) increased liveweight gains with FM even at a liveweight of 500 kg and Waterhouse et al. (1983) with SBM at a liveweight of 320 470 kg. These effects cannot be explained by the ARC 1980 system. One reason for lower protein utilization may be, in addition to low efficiency of mi- crobial synthesis, the poorer amino acid composition in the duodenal digesta on silage than on hay diets (Thomas and Cham- berlain 1982). Following ARC (1980), the apparent ab- sorption of amino acids used in the present calculation was 0.70, which is higher than the 0.63 adjusted for silage diets proposed by Thomas and Chamberlain (1982). Of total DOM, the proportions of OM apparently fermented in the rumen DOM were 74.3, 69.5, 73.5 and 70.6 % for diets PAB, GB, NPS and RSM, which are close to the value presented by Thomas and Chamberlain (1982) for silage diets. They presented a more appropriate equation for microbial synthesis for silage diets, in which the amount of microbial N absorbed from small intestine and deposited in the tissues is 0.41 g N/MJ ME. The corresponding value in the ARC 1980 system is 0.53 for all diets. poorly matched. However, Gill and Eng- land (1983) got a much lower increase in daily gain with sucrose than with FM supple- ments in calves fed silage diet. Thomas et al. (1980) did not observe any effect of barley supplement on the efficiency of microbial synthesis on formic acid silage diet. With protein supplements the efficiency of protein synthesis has sometimes increased (Armstrong 1980). Calculated RDP intake was much higher than requirement, which was also found by Setälä et ai. (1984) in dairy cows fed a simi- lar diet. Degradability of silage crude protein was 84 %, much higher than values reported for formic acid silage in duodenum cannu- lated animals (Thomas et al. 1980, Thomson et al. 1981) and closer to values reported for non-additive silage (Beever et al. 1977). In the present study, calculated amino N flow into the duodenum was much lower than N intake, indicating high ammonia losses from the rumen. Rumen fermentation and blood composition There were no significant differences in rumen pH, total VFA or ammonia N con- centration (Table 6). Ammonia N tended to be higher on RSM and PAB diets than on NPS and GB diets. Total VFA concentration was slightly higher on RSM than on NPS diet indicating more intensive fermentation of RSM diet. The differences in proportions of Efficiency of microbial synthesis in silage diets is lower, because silage OM consists of silage fermentation products and the yield of ATP/kg OMFa is lower (Thomas 1982). Another reason may be that the rates of am- monia and energy release from silage are 81 Table 7. The mean concentration of blood constituents during the experiment. Barley Protein SIM PAB GB NPS RSM Glucose, mmol/1 5.42 5.22 5.37 5.27 0.08 Total protein, g/1 67.3 67.7 68.0 66.9 1.4 Albumin, g/1 37.2 36.7 37.0 36.9 0.5 Urea N, mmol/1 2.23 1.94 1.86a 2.32" 0.12 Ammonia N, mg/100 ml 0.11 0.11 0.10 0.11 0.01 Creatinine, mol/1 95.4 93.9 90.3 99.0 5.2 AFOS», lU/1 257 246 262 244 10.4 ASAT2 ', lU/I 57.8 55.4 55.8 57.5 3.5 Cholesterol, mmol/1 2.47 2.53 2.45 2.55 0.11 11 Alkaline phosphatase, ;i aspartate aminotransferase. Means with different letters were significantly different: a,b (P < 0.05) VFA were small, although significant in the proportion of propionic acid. RSM caused a significant (P < 0.05) in- crease in plasma urea N (Table 7), which agrees with results for other protein supple- ments (Kirby et al. 1983 a, 1983 b, 1984). Gasol treatment tended to decrease plasma urea N content. Fish meal has been found to increase blood glucose concentration (Kirby et al. 1983 b, 1984), but in the present study no effect due to RSM supplement was noted. References Alaviuhkola, T. & Nasi, M. 1984. Gasol-treated barley for pigs. To be published. Anon, 1980. The nutrient requirements of ruminant live- stock. Commonwealth Agricultural Bureaux. 351 p. Armstrong, D.G. 1980. Net efficiencies of microbial N synthesis in ruminant livestock. EAAP-publ. 27 (vol II): 400—411. Bax, J.A. & Offer, N.W. 1982. Measurement of rumen outflow rates and the effect of fish meal on digestibili- ty in young Friesian calves. Anim. Prod. 34: 382. (Abstr.). Beever, D.E., Thomas, D.J., Cammel, S.B. & Harri- son, D.G. 1977. The digestion by sheep of silages made with and without formaldehyde. J. agric. Sci. (Camb.) 88: 61—70. Davis, H.L. & Faichney, G.J. 1973. The effect of form- aldehyde treatment of barley on productive perform- ance of Friesian steers. Aust. J. Exp. Agric. Anim. Husb. 13: 142—145. In conclusion, the present study indicates that RSM can be used as protein supplement on silage diets. RSM supplement increased liveweight gain in growing cattle, which may be the result partly of increased amino acid supply and partly of increased energy intake. RSM increased significantly the ration digest- ibility. A further experiment is needed to evaluate the effect of RSM on silage diets in animals weighing up to 500 kg. Urea supplementation of two forms of barley for in- tensively fed Friesian bulls. Anim. Prod. 38: 551 (Abstr.). Garstang, J.R., Thomas, C. & Gill, M. 1979. The ef- fect of supplementation ofgrass silage with fish meal on intake and performance by British Friesian calves. Anim. Prod. 28: 423 (Abstr.). Gill, M. & England, P. 1984. Effect of degradability of protein supplements on voluntary intake and nitro- gen retention in young cattle fed grass silage. Anim. Prod. 39: 31—36. & England, P. 1983. 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Puolet sekä P-että G-ryhmän eläimistä sai val- kuaistäydennyksenä rypsirouhetta (R) UDP-tarpeen (pötsissä hajoamaton rehuvalkuainen) mukaan, puolet ei saanut valkuaistäydennystä (0). Koe-eläinten keski- määräinen elopaino kokeen alussa oli 106 kg ja ikä 86 pv. Lisäkasvu oli 1066 ja 1070 g/pv ja P- ja G-ryhmällä sekä 1012 ja 1124 g/pv (P < 0.01) 0- ja R-ryhraällä. Vastaavat rehunkulutukset kasvukiloa kohti olivat 3.77, 3.64, 3.75 ja 3.66 ry. Rehun syöntiin rypsirouheella ei ollut vaikutusta. Rypsirouhe paransi merkitsevästi (P < 0.05) orgaanisen aineen, raakavalkuaisen ja raa- kakuidun sulavuutta. Sulavuudet olivat R-ruokinnalla 73.5, 67.4 ja 60.2 % sekä 0-ruokinnalla 71.1, 62.8 ja 57.2 %. Laadultaan säilötyt viljat olivat hyviä. Gasolilla säilö- tyssä ohrassa vesiliukoisen typen osuus oli alempi ja nai- lonpussimenetelmällä määritetty raakavalkuaishävikki oli 9 tunnissa 46.6 % vastaavan hävikin ollessa P- ohralla 76.4 %. Samoin Gasol-käsittely vähensi kuiva- aineen ja tärkkelyksen hajoamisnopeutta pötsissä. Imeytyneiden aminohappojen hyväksikäyttö oli fakto- riaalisti laskettuna eri ruokinnoilla 0.566 ± 0.010. Plasman ureapitoisuus oli R-ruokinnalla korkeampi (P < 0.05) kuin 0-ruokinnalla. Etikkahapon osuus pöt- sinesteen VFA:sta oli R-ryhmällä alempi (P < 0.05) ja propionihapon korkeampi (P < 0.01) kuin 0-ryhmällä. G-ohralla propionihapon osuus VFA:sta oli alempi (P < 0.05) kuin G-ohralla. 1 Nykyinen osoite: Maatalouden tutkimuskeskus 31600 Jokioinen 2 Farmos-Yhtymä Oy Tengströminkatu 6 20360 Turku 84