Maataloustieteellinen A ikakauskirja Vol. 61: 113—122, 1989 Effects of different fertilization practices on the glucose, fructose, sucrose, taste and texture of carrot AINO-MAIJA EVERS Kemira Oy, Espoo Research Centre, Luoteisrinne 2, SF-02270 Espoo, Finland Abstract. The effects of different fertilization practices on the glucose, fructose, sucrose, taste and texture of carrot cv. Nantes Duke Notabene 370 Sv were studied in field experiments carried out in southern Finland in 1985 and 1986. The effects of unirrigated and irrigated place- ment and broadcast fertilization, NPK fertirrigations without basic fertilization, NPK fertirri- gations with NPK basic fertilization, and PK placement with N fertirrigations were compared. Further, the effects of single application were compared with those ofsplit applications. Sug- ar analyses were made by high performance liquid chromatography, and sensory evaluations were performed for taste and texture. The results were analysed by contrast analysis. In 1985, fertilization and fertilization practices had no effects on sugar contents. In 1986, the unfertilized treatments had a tendency to yield higher glucose and fructose, and thus also total sugar contents than did the fertilized treatments on the average. Placement fertilization had a tendency to increase the glucosecontent as compared to NPK fertirrigations. No signifi- cant differences or tendencies were found in sucrose content. The glucose and fructose contents were higher in 1985 than in 1986. The sucrose content was mainly opposite, being higher in 1986.Weather conditions were more favourable for pho- tosynthesis in 1986 than in 1985. The mean total sugar content in fresh weight was 7.8 % in 1985 and 6.7 % in 1986. Indicative results of taste and texture are presented. The sugar con- tent in organically cultivated carrots did not differ from that of conventionally grown carrots, but the taste was worse. Index words: fertilization practices, carrot, quality, sugars, sensory evaluation, taste, texture, organic culture Introduction The sugar contents and flavour of carrot (Daucus carota L.) are influenced by genotype and environment (Simon et al. 1980 a, 1980 b, 1982). Many researchers have reported that year and location have a considerable effect on both overall quality and the sugar contents of carrots (HArdh 1975, Balvoll et al. 1976, Martens 1985). The literature contains contradictory results concerning the influence of fertilization on the 113 JOURNAL OF AGRICULTURAL SCIENCE IN FINLAND https://www.c-info.fi/en/info/?token=3_tsQnEsNC_2lYaQ.5MXhZT3zwVRWFDgOkXv0Mw.Vy__e0y95MexT1XKicoJWGH4bPH2zTvYp3ugPHVklw5kGQziNXk3Gb70WL5163y4nadvE-jld9gZSEiegLnuJXfJOiPhosBXfWIGQzFalfFCpmJCUZSwunm4sTaoW6ShAfmfMCwy_DkW_rLEHDhdYrrEFV3-GTSCf6Lut9Fs sugar contents of carrots. Barnes (1936) reported that increasing nitrogen levels in- creases the amount of glucose, but at the same time he found decreasing sucrose amounts. Habben (1972) reported that increasing nitro- gen levels increases the amount of reducing su- gars (glucose and fructose) but do not affect the sucrose content. According to Dragland (1978), an increasing amount of nitrogen does not effect the glucose content, but decrease the sucrose content of carrots. In Nilsson’s (1979) study, the content of sugars (glucose, fructose, sucrose) in carrots is unaffected by either the type of fertilizer (organic or inorganic) or the amount of fertilizer applied. Barnes (1936) reported that increasing phosphorus levels in- creases the amount of sucrose but do not af- fect the glucose level. Potassium has been noted to decrease the total sugar content and the amount of reducing sugars, and to increase the sucrose content (Habben 1972). On the other hand, Gallagher (1966) reported that potassium has no effect on the sugar contents of carrots. In a review Simon (1985) lists the following components affecting carrot flavour; the free sugars (glucose, fructose, sucrose), which con- tribute to sweetness; volatile mono- and sesquiterpenoids, which contribute harshness and carrot flavour; 2-nonenal, which imparts cooked flavour; “iso-cumarin” and other phenolic compounds, which impart bitterness; 2-methoxy-3-butylpyrazine, which contributes to carrot aroma; free amino acids, which con- tribute to delicate flavour; and ionones, which impart floral off-flavour. The importance of texture to carrot sensory quality has also been noted. Only a few studies have been published con- cerning the effects of fertilization practices or split application on the sugar contents and flavour of carrots. The aim of the present study is to examine the effect of unirrigated and irrigated placement and broadcast fertili- zation, NPK fertirrigations without basic fer- tilization, NPK fertirrigations with NPK basic fertilization, and PK placement with N fertir- rigations on the glucose, fructose and sucrose content and on the taste and texture of car- rots. Material and methods The field experiments were conducted in Vihti, at the Kotkaniemi experimental farm of Kemira Oy (southern Finland, 60°22'N, 24°22'E) during the growing seasons of 1985 and 1986. Carrot cv. Nantes Duke Notabene 370 Sv was grown consistently as reported in Evers (1988). The fertilizer treatments and macronutrient amounts are presented in Ta- ble 1. The field experiment was arranged by completely randomized block design, with four blocks, ten treatments and 25 m 2 plots. Samples for analysis were collected from two blocks in 1985 and from four blocks in 1986. The differences between treatments were studied statistically by contrast analysis and correlations between parameters were calcu- lated (Steel & Torrie 1980). Sugar analyses Carrot samples for glucose, fructose, su- crose analyses and for sensory evaluation of taste and texture were collected at harvest, on 30th September 1985 and 6th October 1986. The sugars mentioned above were determined by high performance liquid chromatography (HPLC) on a bonded aminopropylsilica sta- tionary phase with refractive index (RI) detec- tion (Schwarzenbach 1976). 60 grams carrot tissue (hypocotyl and root tip omitted) was grated and mixed with acetonitril water mix- ture (1:1), crushed in a blender, stirred for 10 minutes, filtered and analyzed by HPLC. The total sugar content given in the Results were calculated as the sum of glucose, fructose and sucrose. The weighed value of the sweetness of sugars was also calculated. The relative sweetness of glucose, fructose and sucrose de- pends on temperature and acidity (Hyvönen et ai. 1978). When the sweetness of sucrose is considered to be 100 at room temperature, the relative sweetness of glucose is 70, and that of fructose 120 (Hyvönen et ai. 1978, Kelm 1985). 114 Table 1. The fertilization treatments and the total amounts of nutrients and irrigation water. 1 Hall' of the nutrients were given in basic placement fertilization and half in NPK fertirrigations. : Phosphorus and potassium were given in basic placement fertilization and nitrogen in fertirrigations. ' The nutrient amounts were 30 % higher in 1985 than 1986. Taste and texture determinations The sensory evaluation was performed at the Technical Research Centre of Finland, Food Research Laboratory. Ten trained tasters evaluated the taste and texture of car- rot samples, in randomized order. They gave their evaluations on numerical and verbal nine-degree Karlsruhe quality scales. Each taster evaluated two carrots per treatment, four treatments at a time. At each session, car- rots from unfertilized and unirrigated treat- ment were present as a comparison sample; thus three evaluation sessions were needed. Because the comparison sample was given somewhat different scores at each of three evaluation sessions, the scores of the second and third evaluation sessions were corrected. This was done by multiplying the scores of each taster and each treatment with the quo- tient comparison sample mean for the first evaluation session/comparison sample mean for the evaluation session in question. Because the residuals of taste and texture grades fol- Organically cultivated carrots Results Sugar contents lowed a normal distribution, it was possible to use parametrical tests for statistical analy- sis. The data were analyzed statistically by contrast analysis. In 1986, carrots were also grown on two or- ganically cultivated fields in central Finland (in Varkaus and Rantasalmi). The same seed material was used, but the geographical situ- ation and soil conditions were different. These samples were not statistically compared to fer- tilizer treatments. The glucose and fructose contents were higher in 1985 than in 1986 (Table 2). The su- crose content was mainly opposite, being higher in 1986. Placement fertilized treatment, Treatment Number and time Macronutrient Irrigation water of fertilizer amounts in 1986' amounts in applications kg/ha 1985 and 1986 N P K Unfertilized Unirrigated 0 0 0 0 0 Irrigation 0 0 0 0 3xlo NPK placement Unirrigated 1 before sowing 80 35 133 0 Irrigation 1 before sowing 80 35 133 3xlo NPK broadcast Unirrigated 1 before sowing 80 35 133 0 Irrigation 1 before sowing 80 35 133 3xlo NPK fertirrigations No basic 3 during season 80 29 160 3xlo Half the basic 1 1 before sowing and 80 32 142 3xlo 3 during season PK placement 2 3N-fertirrigations 1 before sowing and 81 56 133 3xlo 3 during season 4N-fertirrigations 1 before sowing and 155 56 133 4xlo 4 during season 115 116 1 Table 2. The effects of different fertilization practices on the glucose, fructose, sucroseand calculated total sugarcontents and on the calculated value for the sweetness of sugars. Treatment The sugar content, °/o in fresh weight Calculated value ~""for the sweetness Glucose Fructose Sucrose Calculated roi sugars total sugars 1985 1986 x 1985 1986 x 1985 1986 x 1985 1986 x 1985 1986 x Unfertilized 1.9 1.6 1.75 1.7 1.4 1.55 4.1 4.2 4.15 7.7 7.1 7.40 7.5 6.9 7.20 Unfertilized and irrigated 2.0 1.5 1.75 1.8 1.3 1.55 3.6 4.2 3.90 7.4 7.1 7.25 7.1 6.9 7.00 Placement fertilized 2.0 1.5 1.75 1.8 1.3 1.55 4.4 4.1 4.25 8.2 7.0 7.60 7.9 6.8 7.35 Placement fertilized and irrigated 2.2 1.4 1.80 2.1 1.2 1.65 3.6 4.1 3.85 7.9 6.7 7.30 7.6 6.5 7.05 Broadcast fertilized 2.1 1.3 1.70 1.9 1.1 1.50 3.7 4.2 3.95 7.7 6.6 7.15 7.4 6.5 6.95 Broadcast fertilized and irrigated 2.3 1.2 1.85 2.0 1.3 1.60 3.6 3.9 3.75 7.9 6.6 7.25 7.7 6.5 7.10 NPK fertilizations, without basic 2.0 1.5 1.65 1.9 1.2 1.55 3.5 4.1 3.80 7.4 6.5 6.95 7.1 6.4 6.75 NPK fertilizations, half the basic 1.9 1.2 1.55 1.8 1.1 1.45 3.9 4.4 4.15 7.6 6.8 7.20 7.3 6.6 6.95 PK placement with 3N fertirrigations 2.1 1.5 1.80 1.9 1.3 1.60 3.7 3.7 3.70 7.7 6.5 7.10 7.4 6.3 6.85 PK placement with 4N fertirrigations 2.2 1.2 1.70 2.0 1.1 1.55 4.2 4.2 4.20 8.4 6.5 7.45 8.0 6.4 7.20 x 2.1 1.4 1.75 1.9 1.2 1.60 3.8 4.1 4.00 7.8 6.7 7.30 7.5 6.6 7.05 Organically cultivated carrots Location 1 1.5 1.2 4.3 7.0 6.8 Location 2 1.5 1.2 3.9 6.6 6.4 x 1.5 1.2 4.1 6.8 6.6 however, yielded a higher sucrose content in 1985 than in 1986, and PK placement with 3 or 4 N fertirrigations yielded a similar sucrose content in the two years (Table 2). Both the calculated total sugars and the calculated value for the sweetness of sugars were higher in 1985 than in 1986 (Table 2). In 1985, no statistically significant differ- ences were found between fertilized and un- fertilized treatments or between fertilization practices. Irrigation, nitrogen amount, place- ment of NPK or PK fertilizer, fertirrigations or split applications did not affect the sugar contents. In 1986, the unfertilized treatments had a tendency to yield higher glucose and fructose contents than did the fertilized treatments on the average (p<0.08). Placement fertilization had a tendency to increase the glucose con- tent as compared to NPK fertirrigations with- out or with basic fertilization (p