MaataloustieteellinenAikakauskirja Voi. 61: 371—377, 1989 The effect of foliar application of seaweed extract on potato PAAVO KUISMA Potato Research Institute, SF-16900 Lammi, Finland Abstract. The effect of foliar application of commercial seaweed extract on potato growth was studied in long-day conditions (60° 10' N 25°00' E) characterized by a cool and short growing season. The results showed that neither the doses, 0,5, 10, 20 l/ha nor the spraying 24, 45, 58 days after the emergence had any remarkable influence on potato growth and yield if the otherpreconditions of production are in order. Only small, although insignificantbenefit could be obtained with application done three weeks after emergence, clearly before tuber initiation. Index words: seaweed extract Introduction Seaweed extract as a foliar fertilizer has been noted to have various beneficial effects on many crops (Button and Noves 1964, Blun- den 1972). In particular, carbohydrate stor- age crops, such as a sugar beet, benefit remarkably from the use of seaweed extract (Blunden et al. 1974). The use of seaweed ex- tract as a foliar fertilization has also produced a significant increase in the yield of ware pota- toes (Blunden and Wildgoose 1977). In ad- dition, seaweed extract is considered to im- prove the evenness of the size of the tubers, and to give a better cooking, eating and keep- ing quality. It is also thought to give protec- tion from marginal frosts up to —2°C (Chase Organics 1979). From the point of view of plant nutrition, the usual application rates of seaweed extract are so low that the active compound, or com- pounds, must be effective in very low concen- trations. Brain et al. (1973) showed a high cytokinin activity, in a commercial seaweed extract, which is responsible for its many ef- fects. In England, Blunden and Wildgoose (1977) sprayed seaweed extract (SM3) on pota- to crop at the height of 45 cm; this gave a yield increase similar to that with kinetin, which is a derivate of cytokinins. Cytokinins are active at very low concen- trations. They regulate a number of plant functions, including cell division (Koda and Okazawa 1983), protein and C02 metabo- lism, enzyme formation, leaf ageing and senescence, shoot elongation and fruit set (Torrey 1976). The observations of Blunden et al. (1974) that cytokinin treatment augmen- ted the ratio of RNA to DNA suggest that a critical effect of cytokinins in senescence might be the maintenance of theprotein syn- thesizing machinery, perhaps by regulating 371 JOURNAL OF AGRICULTURAL SCIENCE IN FINLAND https://www.c-info.fi/en/info/?token=j50iETn9u3wIhpv4.XiQ6tLVDgK-zOZcnqjN3CA.EuKteS6vOKVIXQyb0ic4XHzl-RPJS_AGcq_7CdD3NLTtOnO8LylWolBv_SnlM_qBJ9AC1BeUAUMv_9bR7Rl_gOUzoaH7I1TRT0cvIqO8eMHF7-oycrYEdVIGW3lt6HQn56C3jlL2QET6Jo3gT5_kIKCHMoLuBz4lswM RNA (Osborne 1962). Cytokinins also pro- mote nutrient mobilization to the points at which they have been applied (Helgeson 1968). On potatoes, cytokinins activate the tuber initiation and growth (Palmer and Smith 1969, Forsline and Langille 1976). In sprouting seed tuber, the content of cytoki- nins increases in the surrounding of sprout bases (Engelbrecht and Bielinska-Czar- necka 1972), and contributes to a break of dormancy (Hemberg 1970, Engelbrecht and Bielinska-Czarnecka 1972). Hartmans and van Es (1979) reported that kinetin, a deri- vate of cytokinins, increased the branching of sprouts on potato. The most beneficial effects on potato growth were reached at a kinetin concentration of 10~6—lo~7 M. The vigor- ous haulm growth which is caused by cytoki- nins, however, does not decrease the tuber yield (Badizadegan et al. 1972). Cytokinins are readily absorbed through leaf surface. Thus they are active only as a foliar application. If cytokinins are applied to the soil, their effects are lost (Brain et al. 1973). Most experiments with seaweed extract have been done in short-day conditions. Vigne and Li (1974), however, recorded that under long-day conditions the applied cytokinins stimulate the stolons to form as air shoots, which causes decrease in yield. The aim of this research was to study the effects of the foliar application of commercial seaweed extract on potato growth and yield under growing con- ditions characterizedby a long day and a cool and short growing season. Material and methods The experiment was conducted by the Department of Plant Husbandry, Helsinki University, on Viikki Experimental Farm (60° 10' N 25°00' E) in 1979. The soil was a sandy loam and fertilized with a drilling fer- tilizer using a chlorine free NPK (8-11-12) at a rate of 1 000 kg/ha (80 kg N/ha). Each plot had two rows, 70 cm apart and 10 m long in a split-plot design with three replications. The chitted seed of the variety Record (30—45 mm) was planted on May 24 with a semiau- tomatic planter at a distance of 30 cm, representing 60 seedtubers a plot. Weed con- trol was accomplished with metribuzin (Sen- cor 0,75 kg/ha) four days after emergence on June 20. Hilling was done on June 28. The trial was harvested on September 27, after the natural senescence of haulms. The seaweed extract was applied at three different times according to the growth stages as main plots: the crop height of 25 cm, the beginning of bloom, and two weeks after the beginning of bloom. The respective spraying dates were 24, 45 and 58 days after emergence on July 10, July 31 and August 15. The doses of seaweed extract SM3 (Chase Org. (G.8.) Ltd.) 0 (water), 5, 10and 201/ha were in sub- plots. They were diluted 0:100, 1:100, 1:50 and 1:25 in water, respectively, and applied as a foliar spray with an experimental sprayer at a volume equivalent to 500 1/ha. According to the analyses of the Finnish State Institute of Agricultural Chemistry (Val- tion Maatalouskemian laitos 1979), the con- stituents of SM3 were as set out hereunder: Nitrogen (N) (Kjeldahl) 0.14 Vo Chlorine (Cl) 2.50 Vo2.50 Vo Calcium (Ca) 0.13 Vo Magnesium (Mg) 0.17 Vo Iron (Fe) 0.0037 Vo Manganese (Mn) 0.0007 Vo lodine (J) 0.083 Vo Selenium (Se) 0.01 ppm According to English analyses (James 1967, Jones 1978), seaweed extract SM3 also contains 0.04—0.24 Vo P, 0.64—2.51 Vo K, 0.02—0.6 Vo amino acids, 1—l5O ppm vita- mins and, e.g., Molybdenum, Vanadium, Germanium, and other elements, but less than 2 ppm in total. Undiluted seaweed extract SM3 has been estimated to have an activity equivalent to 125 mg/litre kinetin in aqueous solution (Blunden and Wildgoose 1977). 372 Growth and yield analyses in order to investigate the development of a stand, the height of three plants was mea- sured once a week from June 25 to the end of August. The maturity of haulms was evalu- ated on a scale of 0— 10(0 = green, 10= dead) on September 3. After the harvest the yields were screened into three groups using 35 mm and 55 mm riddles. Samples, of about 5 kg, for laboratory analyses were taken at random. From the samples the specific gravity was de- termined using the under water weight. This was converted to a starch percentage accord- ing to the table of Halz and Bucholz. The laboratory samples were also used to deter- mine the mean tuber weight by calculating the number of tubers in the sample. The DM con- tent of tubers was determined from 200 g chopped tubers that was dried for 24 h at 100°C. The statistical difference in each treatment was evaluated by the split-plot analysis of vär- iance. In order to get more information about the effects of different spraying doses, the var- iances of subplots were dispersed into variance components: water-seaweed extract means and seaweed extract on the average (Table 1 and 2). The significance levels for the results are shown in the usual manner. Comparisons of means were done according to Tukey’s HSD (Steel and Torrie 1960). Results and discussion When seaweed extract was applied about six weeks after emergence, an increase in stem length was noticed by Blunden and Wild- goose (1977). In this trial the height growth was very slightly affected by the spraying date or dose. In the beginning the potato vines grew a little faster on plots where 20 1/ha seaweed extract had been applied three weeks after emergence, when the plants were about 25 cm high, and the height growth was still vigorous. The influence was strongest a week after ap- Table 1. The analysis of variance of the potato height growth and the haulm senescence. Factor Growth phase between Ist—2nd between 2nd —3rd after 3rd Haulm spraying spraying spraying senes- August 20 cenceJuly 16 July 24 July 30 August 6 August 13 Table 2. The analysis of variance of the potato yield and quality. Spraying dates A ns ns ns ns Spraying doses B ns o ns ns ns ns ns water seaweeds ns * ns ns ns ns ns seaweeds ns ns ns ns ns ns ns AB ns ns ns Factor Tuber Tuber size (mm) Ware Tuber DM Starch yield classes (%) yield size % % t/Ha <35 35-55 >SS t/ha g Spraying dates A ns ns ns ns ns ns ns ns Spraying doses A ns o ns ns ns * ns ns water-seaweed o * ns ns o * ns ns seaweeds ns ns ns ns ns ns ns ns AB ns ns ns ns ns ns ns ns HSD iis 0.3 % 11.9 g 373 plication (Fig. 1). When the spraying was done at the same phase as Blunden and Wild- goose (1977) used, about six weeks after emergence, in the beginning of bloom, or even two weeks later after the end of the height growth, there were no significant differences between application dates or doses. According Vigne and Li (1974), cytokinins the most important compound of seaweed extract easily stimulate the stolons of pota- to crop to form air shoots under a long day. This trial included no estimation of shoot or tuber number. However, the small, although insignificant, acceleration of senescence was seen in potato sprayed with seaweed extract (Table 3). The foliar spray of SM3 seaweed extract did not produce any significant increase in the Table 3. The effects of seaweed extract on the senescence of potato haulms (0 =green, 10=dried). Doses 1/ha Application date Mean July 10 July 31 August 15 5.7 4.3 5.3 5.7 5.3 5.3 5.7 5.3 5.3 5.7 5.0 5.3 5 5.1 10 5.4 20 5.4 Mean 5.3 Untreated 5.1 potato yields at any spraying dates or doses (Fig. 2). In this respect the results are similar to those of Dwelle and Hurley (1984), ob- tained in Aberdeen, Idaho, USA with cv. Rus- set Burbank; in this study foliar application of cytokinins, in seaweed extract or as kine- tin, did not produce any measurable response. In the study of Dwelle and Hurley (1984), the best time for application, however, was later the 2-week period directly following tuber initiation than in this study where the Fig. I. The effects of seaweed extract on the height growth of potato haulms Fig. 2. The effects of seaweed extract on the tuber yield and its size distribution. 374 Table 4. The effects of seaweed extract on the tuber size (g). Table 5. The effects of seaweed extract on ware yield (t/ha). Table 6. The effects of seaweed extract on the DM- content of tubers (Vo). only benefits, although slight, were reached with application done three weeks after emer- gence, clearly before tuber initiation. In disagreement with the results given by Blunden and Wildgoose (1977), the foliar applications of seaweed extract gave, on aver- age, a higher proportion of tubers under 35 mm (Pc.os). This phenomenon was the strongest on the plots sprayed at height of plants about 25 cm. Similarly, the average tu- ber size also was smaller (Table 4). The differ- ences between the doses of seaweed extract can be considered to be minimal. Because of the very small proportion of tubers under 35 mm, the ware yields (tubers over 35 mm) nearly equally followed the gross yields (Table 5). According to Blunden and Wildgoose (1977), the type of response expected from a plant treated with a cytokinin suggests that carbohydrate storage crops would benefit from foliar application of seaweed extract. Trials on sugar beet showed that the plants treated with seaweed extract had a higher root- sugar content than the control plants (Blun- den et al. 1974). In this study, the applied seaweed extract had no effect on the specific gravity or the dry matter content of tubers (Table 6). Dwelle and Hurley (1984) also reported that a foliar application of a com- mercial seaweed extract, CYTEX, in a seven- year study had no significant effect on the specific gravity of potato in any trial year. Humpries (1958) reported that the synthetic cytokinin, kinetin, even depressed both the leaf area and dry matter production of plants of Majestetic potatoes. The results of the experiment show that in long-day conditions, foliar fertilization with seaweed extract has hardly any economical im- portance if the otherpreconditions of produc- tion are in order. Although the seaweed ex- tract was used in nearly the double amount of the recommended application of 11 1/ha, the good status of nutrients in soil was able to cover the effects of the seaweed extract. In this respect, the results agree with the study of Dwelle and Hurley (1984). Some Finnish trials with a foliar fertilization (Anon 1979, 1983, 1984) also show that, in connection of a balanced base fertilization of NPK, seaweed extract given as a foliar application has very slight effects on the yields and starch contents of starch potato. Doses 1/ha Application date Mean July 10 July 31 August 15 5 96.9 93.8 92.0 94.2 10 108.5 107.5 97.2 104.4 20 99.7 96.2 105.1 100.3 Mean 101.7 99.2 98.1 99.6 Untreated 107.2 Doses 1/ha Application date Mean July 10 July 31 August 15 5 39.60 36.60 39.00 38.40 10 39.33 34.90 37.57 37.27 20 39.21 39.30 39.36 39.29 Mean 39.38 36.93 38.64 38.32 Untreated 35.63 Doses l/ha Application date Mean July 10 July 31 August 15 5 20.4 21.3 20.4 20.6 10 20.7 21.1 21.6 21.1 20 19.6 21.0 20.3 20.3 Mean 20.1 21.1 20.8 20.7 Untreated 21.0 375 References Anon 1979. Lehtilannoskoe (A trial of foliar fertiliza- tion). Tärkkelysperunakoetuloksia 1979. p. 76—78. 1983. Lehtilannoituskoe (A trial of foliar fertilization). Tärkkelysperunakoetuloksia 1982. p. 71—74, 1984.Lehtilannoituskoe (A trial of foliar fertilization). Perunantutkimuslaitoksen koetuloksia 1983. Perunan- tutkimuslaitoksen julkaisu 1/1984. p. 61—63. Badizadeoan, M., Tafazoli, E. & Kheradnam, M. 1972. Effect of N6Benzyladenine on vegetative growth and tuber production in potato. Am. Potato J. 49: 109— 116. Blunden, G. 1972. Proc. 7th Int. Seaweed Symp. p. 584. (Ref. Brain et al. 1973). Blunden, G., Wildgoose, P.B. & Nicholson, F.E. 1974, The effect of aqueous seaweed extract on sugar beet. Proc. Bth Int. Seaweed Symp. 3 p. (Manuscript.) Blunden, G. & Wildgoose, P.B. 1977. The effects of aqueous seaweed extract and kinetin on potato yields. J. Sci. Fd Agric. 28: 121—125. Brain, K.R., Chalopin, M.C., Turner, T.D., Blunden, G. & Wildgoose, P.B. 1973. Cytokinin activity of commercial aqueous seaweed extract. Plant Sci. News- letter I: 241—245. Button, E.F. & Noves, C.F. 1964. Effect of seaweed ex- tract upon emergence and survival of seedlings of creeping red fescues. Agron. J. 56: 444. Chase Organics 1979. From the sea CHASE 5.M.3. for potatoes. Liquid extract of seaweed. Chase Organics (G-B) Ltd., Shepperton, England. Leaflet 3 p. Dwelle, R.B. & Hurley, P.J. 1984. The effects of foliar application of cytokinins on potato yields in south- eastern Idaho. Am. Potato J. 64: 293—299. Encelbrecht, 1. & Bielinska-Czarnecka, M. 1972, In- crease of cylokinin activity in potato tubers near end of dormancy. Biochem. Physiol. Pfl. 163: 499—504. Forsline, P.L. & Langille, A.R. 1976. An assessment of modifying of kinetin on in vitro tuberization of in- duced and non-induced tissues of Solarium tubero- sum. Can. J. Bot. 54: 2513—2516. Hartmans, K.J. & Es, A. van 1979. The influence of growth regulators GA3 , ABA, kinetin and lAA on sprout and root growth and plant development using excised potato buds. Potato Res. 22: 319—332. Heloeson, J. 1968. The cytokinins. Science 161; 974— 981. Hemberg, T. 1970. The action of some cytokinins on the rest period and content of acid growth-inhibiting sub- stances in potato. Physiol. Plant. 23: 850—858. Humpries, E.C. 1958. The effects of gibberellic acid and kinetin on the growth of Majestetic potato. Ann. Appi. Biol. 46: 346. James, G.V. 1967. Sample analysis of seaweed extract. Western Counties Laboratories. Mimeogr. 2 p. Jones, R.T. 1978. The assay report of SM3. Portsmouth Polytechnic School of Pharmacy. Mimeogr. 2 p. Koda, Y. & Okazawa, Y. 1983. Characteristic changes in the levels of endogenous plant hormones in rela- tion to the onset of potato tuberization. Japan. J. Crop. Sci. 52: 592—497. Osborne, D.J. 1962. Plant Physiol. 37 (Ref. Blunden et al. 1974.) Palmer, C.E. & Smith, O.E. 1969. Cytokinins and tu- ber initiation in the potato, Solarium tuberosum L. Nature (London) 221: 279—280. Steel, R.G.D. & Torrie, J.H. 1960. Principles and procedures of statistics. 481 p. McCraw-Hill, New York. Torrev, J.G. 1976. Root hormones and plant growth. Ann. Rev. Plant Physiol. 27: 435—459. Valtion Maatalouskemian laitos 1979. Tulkimustodistus No. A 592/79. I p. Vicne, J.T. & Li, P.H. 1974. The effect of simazine and cytokinins on potato tuber protein, nitrogen content and yield. Potato Res. 17: 327—332. Ms received June 8, 1988 SELOSTUS Merileväuutteen vaikutus lehtilannoitteena perunan satoon Paavo Kuisma Perunanlutkimuslailos, 16900 Lammi Merileväuutteen käytön lehtilannoitteena on havaittu tuottavan monia edullisia vaikutuksia useiden viljelykas- vien kasvuun ja satoon. Erityisesti runsaasti hiilihydraat- teja varastoivien kasvien kuten sokerijuurikkaan on to- dettu hyötyvän merkittävästi merileväuutelannoitukses- ta. Eräänä selityksenä merileväuutteen hyviin vaikutuk- 376 siin kasvien sadonmuodostuksessa on esitetty sen korke- aa sytokiniinipitoisuutta. Perunassa sytokiniinit mm. voi- mistavat mukulanmuodostusta ja varsiston kasvua sekä parantavat ravinteiden hyväksikäyttöä. Merileväuutteen käyttöä perunan lehtilannoitteena tut- kittiin Viikissä 1979. CHASE SM3 -merileväuutteen käyt- tömäärät vastasivat 0,5, 10 ja 20 l:aa/ha sekoitettuna 500 l:aan vettä/ha. Käsittelyajankohdal olivat 24, 45 ja 58 päivää taimettumisesta. Koe osoitti, että hyvissä kasvu- oloissa käytettäessä keväällä istutusvaiheessa tasapainoista lannoitusta (1 000 kg Ylkv/ha) merileväuutteen hyöty leh- tilannoitteena on hyvin vähäinen. Vaikka uutetta käytet- tiin lähes kaksinkertainen annos (20 l/ha) suositellusta (11 l/ha), kasvualustan luontaisesti hyvä ravinnetila ky- keni peittämään merileväuutteen mahdolliset lehlilannoi- tusvaikutukset. Ainoa vähäinen hyöty saavutettiin, kun käsittely suoritettiin kolmen viikon kuluttua taimettumi- sesta, vielä selvästi ennen mukulanmuodostuksen alka- mista. 377