Maataloustieteellinen A ikakauskirja Vol. 63: 443—453, 1991 Effects of yeast culture supplement on digestion of nutrients and rumen fermentation in cattle fed on grass silage barley diet PEKKA HUHTANEN Department of Animal Husbandry, University of Helsinki SF-00710 Helsinki, Finland Abstract. The effects of including yeast culture (Saccharomyces cerevisiae plus growth medium; 5 x 106 organisms/g) on the digestion of dietary constituents in the rumen and to- tal digestive tract were studied in a pair of monozygote twin bulls. The animals were fitted with cannulae in the rumen and in the proximal duodenum. A diet of grass silage, barley and rapeseed meal (445, 445 and 90 g/kg total dry matter (DM)) was fed, with and without addi- tion of 10 g per day of yeast culture (YC), in two treatment sequences. The addition ofYC had no effect on the mean values of rumen pH, ammonia N concen- tration or molar proportions of volatile fatty acids. Also, the postprandial changes in rumen fermentation pattern were similar when the diet did and did not contain the YC supplement. The peak concentration of lactic acid 1 h after feeding tended to be higher in cattle receiving the YC diet (13.9 v 6.0 mmol/1). Apparent digestibility of organic matter (OM) (mean 0.780) and the proportion of OM digestion occurring in the rumen (mean 0.603) were not affected by YC. Likewise, there was no effect on rumen or total digestion of cell wall carbohydrates, and the results for the degra- dation of hay DM in the rumen and for particle-associated carboxymethylcellulase and xylanase activities indicated that YC had no effect on the rumen environment that could affect fibre digestion. Supplemental yeast did not affect the rate of microbial N synthesis (28.0 and 28.6 g/kg OM apparently digested in the rumen). The results indicate that the addition of YC to the diet is not likely to improve the effi- ciency of digestion and fermentation in the rumen of cattle given a diet based on grass silage and barley. Index words: ruminant, yeast culture, digestion, rumen fermentation Introduction Brewer’s by-products, which contain vary- ing amounts of live and dead yeast cells (Sac- charomyces cerevisiae), have been used as a supplementary protein source for dairy cows (Steckley el al. 1979). Recently, much data relating to the use of small amounts of live yeast cultures (YC; Saccharomyces cerevisiae) as probiotics in ruminant diets have been 443 JOURNAL OF AGRICULTURAL SCIENCE IN FINLAND https://www.c-info.fi/en/info/?token=sb4qTAJvcs80F4m6.WyurbMZIna6bHE9Hru8Fmg.sCkfKApoat8vxp2W680Isqf417LFO-Fex529pl_sDcSjIlaR7toEQLkp989dHJyWh0srrIIOWYK3oX7zSkgrR99hkhj1AD4X-8eXovfTEbV1W9BS0knpm3ndsScust2YWPV390mc8BzUjCUaQzttltDQya--bgvX8Qql-FVh7pPiR0b8tAgd62JoeylJzUToiKSLLu582hGvQDo2V0THWwPbH7YuRunsbkLyEOdc8w7fThgdxFY0f5x4TmOkLVnOB7tF8x8 reported. Dawson and Newman (1987) ob- served viable yeast cells in continuous fermen- ters receiving yeast supplement for up to 14 h after feeding. On the other hand, Arambel and Rungsyin Tung (1987) concluded that YC cannot maintain a productive population within the rumen ecosystem. The results of Newbold et al. (1990) were inconclusive with regard to demonstrating yeast growth in the rumen; the decline in viable cell numbers with time after dosing was, at 0.17/h, close to the expected rate of rumen outflow. Gunther (1989) reported addition of YC to the diet to result in proportional increases of 7.6, 16.8 and 15.2 % in milk, fat and pro- tein yield in dairy cows producing approxi- mately 30 kg/d. Williams (1989) reported, using four complete mixed diets to test the effects of YC, the average milk yield to in- crease by 0.8 kg/d and the fat corrected milk (FCM) yield by 1.6 kg/d. The effects were only observed at high levels of concentrate in the diet. With diets of grass silage and barley Bax (1988) reported a mean increase of 0.4 kg FCM/d, the response being greater when si- lage and barley were given separately rather than as a complete mixed diet. In two other experiments in which cows were given total mixed rations supplemented with YC no effect on milk yield was obtained (Harris and Lobo 1988; Quinonez et al. 1988). The effects of YC on milk composition have been varied: milk fat content has increased (Teh et al. 1987; Gunther 1989), the effect has varied with the diet (Williams 1989) or else no effect has been observed (Bax 1988; Harris and Lobo 1988; Quinonez et al. 1988; Huber et al. 1989). Gunther (1989) reported a large increase in milk protein content in cows receiv- ing YC, whilst Bax (1988) and Harris and Lobo (1989) found no effect, and Huber et al. (1989) reported that YC decreased milk protein content in a large-scale trial. The mode of action of yeast cultures has been explained by their ability to alter rumen function. Supplementation of YC has been reported to alter the rumen fermentation pat- tern (Wiedmeier et al. 1987; Teh et al. 1987; Harrison et al. 1988; Martin et al. 1989; Williams 1989) and to increase ruminal counts of cellulolytic bacteria (Wiedmeier et al. 1987; Dawson and Newman 1987; Harri- son et al. 1988). Increases in the number of cellulolytic bacteria did not correspond to in- creases in cell wall digestion in vivo, however (Harrison et al. 1988). The addition of YC to the diet has also changed the extent and rate of digestion in the rumen (Wiedmeier et al. 1987; Williams 1989; Gomez-Alarcon et al. 1990). Williams (1989) suggested that the ac- tion of YC may partly be related to allevia- tion of a negative associative effect of concen- trate supplementation. The pH elevation of 0.5 units with YC was related to a reduction in postprandial concentration of lactic acid in cattle given a diet of hay and barley. The results indicate that there has been large vari- ation in response to YC supplementation both in animal performance and in rumen function. This variation may be related to the type of diet used in testing the products and also to differences in the products tested. Most tests of the effects of YC have been carried out with diets not used in Finland. Ac- cordingly, this experiment was designed to study the effects of YC on rumen metabolism and digestion of yeast culture (Yea-SACC 1; Saccharomyces cerevisiae plus growth medi- um; 5 x 106 organisms/g) in a typical Finn- ish diet for cattle containing grass silage and barley. A high level of concentrate supplemen- tation was used to monitor any effect of YC in alleviating the possible negative associative effect of a high concentrate diet. Material and methods Animals The test animals were a pair of monozygote bulls of Hereford xFriesian breed (initial live weight 430 kg). Each had a permanent rumen cannula and a simple T-piece cannula fitted 1 Yeast culture, YEA-SACC, Alltech Biotechnology Center, 3031 Catnip Hill Pike Nicholasville, Kentucky, KY 40356. 444 in the proximal duodenum. The animals were ery 2 h were pooled to provide one composite kept in metabolism cages. Diets and design The experiment was conducted on a switch- over design. The animals received a daily al- lowance of 8.6 kg DM of a diet composed on DM basis (g/kg) of grass silage 455, barley 455 and rapeseed meal 90, withand without a sup- plementation of 10g/d of yeast culture (YC). The animals were allocated at random to the two treatment sequences, either control diet followed by YC or the reverse. The direct-cut grass was ensiled with a formic acid based ad- ditive (AIV II; 800 g formic acid/kg) applied at the rate of 5 1/t. Feed was offered in two equal meals at 7.00 and 19.00 hours through- out the experiment. The daily dose of YC was introduced into the rumen via the cannula in two equal portions (5 g) at the beginning of each feeding. Water was freely available and a commercial mineral mixture was given at the rate of 150 g/d. Experimental procedures Each experimental period consisted of a 14-day adaptation period followed by a 14-day period when faecal collections, rumen and duodenal samplings, nylon bag incubation and determination of the activities of polysac- charide-degrading enzymes were made. The procedures for faecal collection, rumen and duodenal sampling and preparing the samples were as described by Huhtanen (1988). Rumen sampling was done on two days of each period (25 and 27). Cr-mor- danted straw and LiCoEDTA (Uden et al. 1980) were used as digesta flow markers, and purine bases (Zinn and Owens 1986) as microbial N marker. A graphic alternative of Faichney’s (1975) double-marker method (McAllan and Smith 1983) was used to cal- culate the flows of dietary constituents at the duodenum. For counting the number of ru- men protozoa, individual samples taken ev- sample per day for each animal. The samples were stored in formalin. Triplicate samples of 3.5 g of hay ground to pass a 2-mm screen were incubated in ny- lon bags (pore size 41 pm, external dimensions 120 and 60 mm) for 6, 12, 24, 48 and 72 h in the rumen of each animal starting on days 16 and 23 of each period. This made 12 bags for each incubation period and treatment (2 days x 2 periods x 3 bags). The equations of McDonald (1981) were fitted to the datarelat- ing to the disappearance of DM from the bags in order to estimate degradation constants a (rapidly digested soluble fraction), b (degrad- able fraction), c (rate of degradation of com- ponent b) and lag time (time elapsed before the start of the degradation). To measure particle-associated carboxy- methylcellulase (CMCase) and xylanase activi- ties, duplicate samples of 0.6 g of hay were incubated in the rumen for 3,6, 12, 24 and 48 h. After withdrawal from the rumen the bags were washed in cold water in a house hold washing machine. The length of the washing procedure was 30 min consisting of 5 rinsing cycles. After washing, the bags were squeezed thoroughly. The duplicate bags were pooled before extraction of the enzymes by a procedure similar to that used by Nossal and Heppel (1966). The details of the procedure have been described by Silva et al. (1987). Representative samples of approximately 1.0 kg of rumen digesta were taken on day 26 of each period. The sample was first divided into two subsamples, of which one was homo- genized, and the other was strained through a cheese-cloth to obtain strained rumen fluid (SRF). Rumen particulate material (RPM) was obtained by washing the sample remaining on the cheese-cloth, using a similarprocedure to that used for rumen incubated nylon bags. Duplicate samples of RPM (2 g), homogeniz- ed rumen digesta (HRD; 5 g) and SRF (5 ml) were used for enzyme extraction. The DM content of RPM and HRD samples was de- termined at 103°C for 24 h. 445 Chemical analysis Methods of chemical analysis were as described by Huhtanen (1988). The chemical composition of the experimental feeds is presented in Table 1. The concentration of D( —), L( + ) -lactic acid in rumen fluid was determined using lactic dehydrogenase in a test combination kit (Boehringer Mannheim GMDH). Protozoal counts were made using a haemacytometer after fixing with methyl green formalin saline (MFS) solution. The details of the determinationof CMCase activity are described by Groleau and Fors- berg (1981). Xylanase activity was assayed by the same procedure as CMCase but with xy- lan (Sigma No. X-0376) as substrate. The sam- ples were centrifuged at 800 x g for 5 min be- fore the absorbance was read. Statistical analysis The data were analyzed by two way analy- sis of variance (treatment, period). For the residual sums of squares there remained 1 de- gree of freedom. Rumen fermentation data were analyzed by a split-plot analysis of vari- ance (Snedecor and Cochran 1969). Table 1. Chemical composition of the experimental feeds (g/kg DM). DM Ash N NDF ADF ADL (g/kg) Grass silage 1 245 76 24.1 542 283 17 Barley 866 26 22.5 209 61 7 Rapeseed meal 877 83 54.0 281 184 75 In silage: pH 3.75; in DM (g/kg): water-soluble carbo- hydrates 37, lactic acid 67, acetic acid 19, butyric acid 0.4; in total N (g/kg): ammonia N 35, soluble N 454. Results Rumen fermentation Daily values (mean, minimum, maximum and range) for rumen pH, and for the con- centrations of ammonia N, lactic acid and volatile fatty acids (VFA) are shown in Table 2. Supplementation of the diet with YC had virtually no effect on rumen fermentation. Also, postprandial changes in rumen pH, am- monia N and molar proportions of VFA were similar for the two treatments (time x diet in- teraction P>0.05). The postprandial increase in the molar proportion of propionate and de- crease in that of acetate were slightly greater when YC was included in the diet, resulting in a larger diurnal variation in the acetate to Table 2. Effects of yeast culture(YEA-SACC) on rumen fermentation in the cattle. The values are means of 8 samp- ling times except for lactic acid (4 samplings at o—3 after feeding). Control Yeast culture .SEM Mean Min Max Range Mean Min Max Range pH 5.90 5.62 6.52 0.90 5.97 5.61 6.43 0.82 0.053 Ammonia N (mmol/1) 11.50 5.14 17.79 12.36 12.50 6.36 19.07 12.71 0.391 Lactic acid (mmol/1) 1.9 0.1 6.5 6.4 5.3 0.1 13.9 13.8 0.90 Total VFA (mmol/1) 135.8 118.3 152.3 34.0 134.8 117.0 155.0 38.0 2.50 Molar proportions of VFA (mmol/1) Acetic acid 624 605 652 47 626 593 664 71 1.9 Propionic acid 173 163 182 19 169 152 182 30 6.5 Isobutyric acid 11.5 11.0 12.3 1.3 11.4 10.1 12.5 2.4 0.65 Butyric acid 155 142 163 21 159 140 175 35 4.6 Isovaleric acid 13.3 11.9 14.6 2.7 15.1 13.3 16.7 3.4 1.25 Valeric acid 16.6 13.8 20.3 6.5 15.6 11.6 20.2 8.6 0.41 Caproic acid 6.0 4.6 7.9 3.3 4.4 3.4 5.2 1.8 1.49 446 propionate ratio. The rumen acetate to propi- onate ratio varied by 0.67 units over the day when the cattle were given the control dietand by 1.10 units when they were given the YC supplement. The mean acetate to propionate ratio was 3.63 and 3.75 for the control and YC diets, respectively. When receiving the YC supplement the cattle tended (P < 0.1) to have a higher ruminal concentration of lactic acid. The trend was similar for both L( + ) and D( —) isomers of lactic acid (Fig. 1). The num- ber of rumen protozoa was 10% higher when the cattle were fed the YC diet than when they were fed the control diet (7.43 x 105 vs. 6.76 X 105 ; SEM = 0.094). Digestion of organic matter and cell wall carbohydrates Organic matter (OM) intake was lower with the control diet because during the first peri- od the animal fed on this diet did not consume all its feed (Table 3). Neither rumen and total digestion of OM nor the proportion of diges- tion occurring in the rumen were changed by YC supplementation. Addition of YC in- creased the digesta flow at the duodenum by 12 % (105.4 vs. 93.8 kg/d; SEM 3.15). How- ever, the difference was only 3 % when the digesta flow was calculated per kg DM intake. The results for the digestion of cell wall car- bohydrates are given in Table 4. Rumen and total digestion of different cell wall consti- tuents were virtually similar between the treatments. Addition of YC had no effect on the extent of the DM degradation of hay, but it increased the rate of degradation of from 0.0364 to 0.0475/h and increased the lag time of degradation (Table 5). Only after an incu- bation period of 48 h did YC supplementa- tion show a trend towards greater disappear- ance of DM. Table 3. Effects of yeast culture (YEA-SACC) on the digestion of organic matter (OM) by the cattle. Diet OM (kg/24 h) Apparent digestibility In feed At duodenum In faeces Rumen Total Rumen/total Control 7.50 4.00 1.64 0.469 0.781 0.600 Yeast culture 8.00 4,27 1.78 0.472 0.780 0.605 SEM 0.473 0.287 0.145 0.0057 0.0051 0.0033 447 Fig. I. The effect of yeast culture on post-prandial changes in the concentration of lactic acid in the rumen (C control, YC = yeast culture). Table 4. Effects of yeast culture (YEA-SACC) on the rumen and total digestibility of cell wall carbohydrates by the cattle. Cell wall constituent Control Yeast culture SEM NDF Rumen 0.669 0.666 0.0009 Total 0.686 0.692 0.0102 Rumen/total 0.971 0.966 0.0131 ADF Rumen 0.625 0.606 0.0091 Total 0.644 0.647 0.0125 Rumen/total 0.972 0.937 0.0044 Cellulose Rumen 0.690 0.672 0.0142 Total 0.714 0.715 0.0128 Rumen/total 0.968 0.939 0.0027 Hemicellulose Rumen 0.706 0.719 0.0068 Total 0.729 0.726 0.0082 Rumen/total 0.970 0.990 0.0203 Digestion of nitrogen The results for the digestion of nitrogenous constituents are given in Table 6. The quanti- ties of total N, non-ammonia N (NAN) and microbial N entering the small intestine were slightly higher when the cattle were given the YC supplement than when they were given the control diet alone, mainly because of the difference in feed intake. Neither apparent nor true efficiency of microbial N synthesis in the rumen was affected by YC. Likewise, addi- tion of YC had no effect on the ratio of pu- rine N to total N in isolated rumen bacteria (0.140 vs. 0.138) and therefore the flow of microbial N reflected a similar pattern to pu- rine N. YC had no effect on either apparent digestibility of N or faecal output of purine N as RNA (ribonucleic acid) equivalents. Enzyme activities The effect of YC on the activities of CMCase and xylanase extracted from mi- Table 5. Effects of yeast culture (YEA-SACC) on the dis- appearance of DM (mg/g) from nylon bags incubated in the rumen of the cattle and degradationconstants (a, b, c and lag time). Control Yeast SEM culture Incubation period 6 h 358 356 8.9 12 h 441 465 3.9 24 h 578 587 17.3 48 h 670 714 5.8 72 h 749 757 4.8 a 258 229 1 b 522 548 ' c 0.0364 0.0451 ' Lag time (h) 1.4 2.3 1 1 Degradation constants calculated using the average values of the two animals 448 Table 6. Effects of yeast culture (YEA-SACC) on the digestion of nitrogen (N) by the cattle. Control Yeast SEM culture Nitrogen (g/24 h) In feed 204.5 222.8 14.4 At duodenum Total N 223.0 231.4 22.7 Ammonia N 4.5 4.4 1.8 Non-ammonia N 218.5 226.9 20.9 Microbial N 98.3 109.1 9.7 In Faeces Total N 55.5 58.9 3.10 Purine N' 22.2 22.5 1.56 Microbial N (g/kg OMADR2 ) 28.0 28.6 1.31 (g/kg OMTDR3 ) 21.5 21.8 0.77 1 RNA equivalents 2 Organic matter apparently digested in the rumen 3 Organic matter truly digested in the rumen crobes associated with feed particles incubat- ed in nylon bags for different periods of time is shown in Fig 2. The differences were not statistically significant after any period of in- cubation. The activities of polysaccharide-degrading enzymes extracted from total rumen microbial population (HRD) and from a population firmly associated with feed particles were simi- lar for the control and YC treatments (Fig. 3). The activities of CMCase in SRF were 0.57 and 0.36 pmol/ml per min for the control and YC treatments, and those of xylanase 0.67 and 0.55 pmol/ml per min, respectively. Discussion In agreement with the observations of Adams et al. (1981) and Wiedmeier et al. (1987), YC had no effect on rumen pH. In contrast, Williams et al. (1989) reported an increase and Harrison et al. (1988) a de- crease in rumen pH due to yeast supplemen- tation. The reasons for these variable results are unclear; in the present study and in those of Harrison et al. (1988) and Williams (1989) the level of concentrate was high. The effect of YC on pH has also been variable in vitro (Dawson and Newman 1987, Martin et al. 1989). If the action of YC is related to the alleviation of the negative associative effect between forage and concentrate (Williams 1989), it would be most likely to occur with a high concentrate diet such as used in this study. The postprandial concentration of lac- tic acid in the rumen was found to higher in cattle given YC, in contrast to the observa- tions of Williams (1989) and Newbold et al. (1990). Although the difference did not reach statistical significance, the concentration of both isomers of lactic acid was consistently higher on both sampling days with YC. The ruminal VFA pattern was not altered in the present study. In other studies the ef- fects of YC on rumen fermentation have been variable. Harrison et al. (1988), Williams (1989), Martin et al. (1989) and Newbold et Fig. 2. The effect of yeast culture on CMCase and xylanase activities extracted from microbes associated with feed particles incubated nylon bags (C = control, YC = yeast culture). Fig. 3. The effect of yeast culture on CMCase and xyla- nase activities ofhomogenizedrumen digesta (HRD) and rumen particulate material (RPM) (C = control, YC = yeast culture). 449 al. (1990) reported that theaddition of YC de- creased the acetate to propionate ratio in ru- men VFA. On the other hand, Williams (1987) and Teh et al. (1987) reported a higher acetate to propionate ratio in rumen VFA in animals receiving diets supplemented with YC. The variable responses to YC supplementation in milk fat content (Teh et at. 1987; Gunt- her 1989; Williams 1989) also indicate that the effects of YC on the rumen fermentation pattern are unpredictable. In the present study, in contrast to that of Williams (1989), no reduction in methane production in cattle fed YC would be expected on the basis of ru- men fermentation stoichiometry. The present study gave no evidence for a stabilizing effect of YC on rumen fermenta- tion, in contrast to the observations of Har- rison et al. (1988) and Williams (1989). For most of the fermentation parameters the diur- nal variation was even slightly greater when YC was included in the diet. Supplementing the silage diet with barley leads to a marked increase in the total number of rumen pro- tozoa (Chamberlain et al. 1985; Jaakkola and Huhtanen 1990), which should also stabilize rumen fermentation. Ushida et al. (1988) reported that rumen protozoa had a greater effect on cell wall digestion in animals receiving forage plus maize starch diet than in those fed on forage alone. The total num- ber of rumen protozoa (7 x 10s) was 37 and 20 % higher than observed by Jaakkola and Huhtanen (1990) when the level of barley was 250 and 500 g/kg total DM in grass si- lage based diet. In agreement with Adams et al. (1981), Williams (1989) and Gomez-Alarcon et al. (1990), YC had no effect on the total digesti- bility of OM. Wiedmeier et al. (1987) ob- served increases over control in digestibility of crude protein and hemicellulose with YC, and Gomez-Alarcon et al. (1990) in that of crude protein, NDF and ADF. Harrison et al. (1988) reported inclusion of YC in the diet to result in non-significant decreases of 5.1 and 8.4 %-units in the digestibility of DM and NDF. They also found that in vitro cellulose disappearance was lower for donoranimals re- ceiving YC than for controls, despite the greater counts of cellulolytic bacteria. In con- trast to the observations of Gomez-Alarcon et al. (1990), YC had no effect on rumen digestion of OM or cell wall constituents in the present study. The absence of response to YC in the proportion of OM or cell wall diges- tion occurring in the rumen was consistent with theresults for DM degradation in the ru- men in nylon bags and for the activities of particle-associated polysaccharide-degrading enzymes. Faecal output of purine bases was similar for all the treatments indicating that there were no quantitative differences in the production of microbialprotein in the hid-gut, and hence in the amount of carbohydrates fer- mented in the lower tract. The effect of YC on degradation parameters has been variable; Williams (1989) reported a shorter lag time of digestion, a slower rate and lower extent of degradation with YC, whereas in the pres- ent study the lag time was longer and the rate of digestion faster with YC. However, the different effects of YC on degradation para- meters may counterbalance each other so that the total effect on rumen or total digestion of cell wall carbohydrates is likely to be small. The absence of a difference in rumen micro- bial protein production between the treat- ments was consistent with the same mean ru- men ammonia N concentration and similar pattern of diurnal changes. This was also in agreement with Gomez-Alarcon el al. (1990), who did not find any effect of the addition of YC on the efficiency of microbial N synthesis in the rumen. Although there are many factors which might influence on rumen ammonia concentration, in thepresent experi- ment any effect of YC on rumen microbial protein production should be reflected in am- monia N concentration. The two factors, in addition to net incorporation by rumen mi- crobes, which may influence on rumen ammo- nia concentration, i.e. rumen pH through am- monia absorption (Siddons et al. 1985) and protozoal number through increased intraru- minal recycling of N (Coleman 1975), were 450 similar in the two treatments. In other studies both decreases (Dawson and Newman 1987, Harrison et al. 1988, Newbold et al. 1990) and increases (Arambel et al. 1987; Martin et al. 1989) in rumen ammonia concentration have been reported. YC had no effect on particle-associated CMCase and xylanase activities, extracted ei- ther from feed particles incubated in nylon bags or from rumen particulate matter, in agreement with the absence of an effect on ei- ther in vivo or in sacco digestion of cell wall carbohydrates. Particle-associated CMCase and xylanase activities have proved very sen- sitive indicators of differences in rumen en- vironment that could affect fibre digestion (Silva et al. 1987; Huhtanen and Khalili 1989). In other studies YC has increased the number of cellulolytic bacteria in the rumen (Dawson and Newman 1987; Wiedmeier et al. 1987; Harrison et al. 1988). Harrison et al. (1988) suggested that because cell wall diges- tion in vivo and cellulose digestion in vitro were reduced with YC, the activity of cellulo- lytic bacteria was somehow decreased. In conclusion, the experiment reported here suggests that no improvements can be expect- ed in the efficiency of rumen metabolism from supplementation of grass silage plus barley diet with yeast culture. This may be because of the large number of rumen protozoa with this kind of diet, which stabilize rumen fer- mentation. Grass silage also contains a large number of viable yeast cells, especially when ensiled with formic acid (Henderson et al. 1972). Assuming yeast cells numbering of 1 X 105 /g of wet silage, then in the present study the animals consumed 1.60 x 109 yeast cells/d. This is approximately 32 times the number of yeast cells added by feeding 10 g/d of YC (5 x 107 yeast cells). The proportion of Saccharomyces cerevisiae among silage yeasts is nevertheless generally small (Wool- ford 1984). Acknowledgements. The author thanks Mrs Cecilia Rosenlew-Rydback, Ms Leena Laitinen and Mr Juha Suo- mi for skilled technical assistance, Ms Ulla Kukkonen for counting rumen protozoal number and Mr Martti Tala for surgical preparation of the experimental animals. The partial support given by Alltech Biotechnology, Inc., Nicholasville, KY, USA, is gratefully acknowledged. References Adams, D.C., Galyean, M.L., Kiesling, H.E., Wal- lace, J.D. & Finkner, M.D. 1981. Influence of via- ble yeast culture, sodium bicarbonate and monensin on liquid dilution rate, rumen fermentation and feed- lot performanceof growing steers and digestibility in lambs. J. Anim. Sci. 53: 780—789. Arambel, M.J. & Runo-syin, Tung 1987. Evaluation of Saccharomyces cerevisiae growth in the rumen eco- system. 19th Biennial Conference on Rumen Func- tion. p. 29. Chicago, Illinois. —, M.J., Wiedmeier, R.D. & Walters, J.L. 1987. 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Ms received December 21, 1990 452 SELOSTUS Elavan hiivavalmisteen (YEA-SACC) vaikutus ravintoaineiden sulatukseen ja pdtsifermentaatioon saildrehuvaltaisella ruokinnalla Pekka Huhtanen Helsingin yliopisto, kolielainlieteen laitos, 00710 Helsinki Elavan hiivavalmisteen (Saccharomyces cerevisiae + kasvatusalusta; 5 x 106 organismia/g) vaikutusta diee- tin eri komponenttien sulatukseen ja pdtsifermentaatioon tutkittiin identtisilla kaksossonneilla, joilla oli pdtsi- ja ohutsuolifisteli. Ruokinta koostui saildrehusta, ohrasta ja rypsirouheesta (445, 445 ja 90 g/kgkuiva-ainetta (ka)). Koeruokinnalla annettiin lisaksi 10g/pv hiivavalmistet- ta (YEA-SACC). Koejarjestelyna oli switchover -koe, jos- sa toinen sonneista oli 1. jaksolla kontrolli- ja toinen koeruokinnalla ja 2. jaksolla painvastoin. Koejakson pituus oli 28 pv. Hiivalla ei ollut vaikutusta pdtsinesteen keskimaaraiseen pH:hon, ammoniakkipitoisuuteen tai haihtuvien rasva- happojen mooliosuuksiin. Mydskaan ruokinnan jalkeiset muutokset ym. potsifermentaatiotakuvaavissa paramet- reissa olivat samanlaisia. Ruokinnan jalkeinen (1 h) pdt- sin maitohappopitoisuus oli hiivaa saaneilla sonneilla suurempi (13.9 vs. 6.0 mmol/1). Hiivalla ei ollut vaikutusta orgaanisen aineen naen- naiseen sulavuuteen (keskimaarin 0.780) eika pdtsisulatuk- sen osuuteen (keskimaarin 0.603). Hiiva ei mydskaan vai- kuttanut solunseinamahiilihydraattien pdtsi- tai kokonais- sulavuuteen. Nailonpussimenetelmalla maaritetyn heinan kuiva-aineen pdtsihajoavuuden tai partikkeleihin kiinnit- tyneiden mikrobien karboksimetyylisellulaasi- ja xyla- naasiaktiivisuuksien perusteella arvioituna hiiva ei vaikut- tanut kuidun sulatukseen pdtsissa. Mikrobiproteiinin tuotantoon pdtsissa hiivalla ei ollut vaikutusta. Taman kokeen tulosten perusteella hiivavalmisteella ei saildrehuun perustuvalla ruokinnalla ole sellaista pot- simetaboliaa tehostavaa vaikutusta, jonkaperusteella olisi odotettavissa tuotoksen lisayksia kasvavilla naudoilla tai lypsylehmilla. Syyna saattaa olla pdtsin runsas alkuelain- ten maara saildrehu-ohraruokinnalla, mika tasoittaa pdt- sikaymista. Lisaksi elainten saildrehussa saamien hiiva- solujen maara on moninkertainen hiivavalmisteessa saa- tuun maaraan verrattuna. 453