Voi 4:363-375. Leaching and uptake of nitrogen and phosphorus from cow slurry and fox manure in a lysimeter trial Erkki Kemppainen Agricultural Research Centre ofFinland, FIN-31600 Jokioinen, Finland The effects of soil type, spreading time and use of a nitrification inhibitor (Didin) on the leaching and uptake of nitrogen and phosphorus from cow slurry and fox manure were studied in a lysimeter trial during 1989-1992. The runoff volume and leaching of total nitrogen in peat soil were double those in fine sand soil. There was no essential difference in the leaching of nitrate nitrogen, but the amount of ammonium nitrogen leached from peat soil was 50-fold and that of organic nitrogen about 3-fold the amounts leached from fine sand soil. The amount of total phosphorus leached from peat soil was 23-fold and that of soluble phosphorus 39-fold the amounts leached from fine sand soil. There were only minor differences between cow slurry and fox manure in the leaching of nitrogen. The least nitrogen was leached after manure application in May. Didin did not have any significant effect. In peat soil, significantly more phosphorus leached from fox manure than from cow slurry. Spreading time had no effect on phosphorus leaching. Considerably higher barley grain yields were harvested from peat soil than from fine sand soil. Fox manure proved to be a much more effective fertilizer than cow slurry. In the first experimental year, December spreading resulted in a lower yield than other spreading times. Of the total nitrogen ap- plied in manure, 24-41% was recovered in barley yield (grain+straw) and 4-21% in runoff; the fig- ures for phosphorus were 6-51% and 0-2,6%, respectively. Key words: peat soil, fine sand soil, spreading time, nitrification, nitrification inhibitor, nitrate, am- monium, organic nitrogen, soluble phosphorus, runoff Introduction The leaching ofnutrients from livestock manures has been studied rather little in Finland. Yet re- sults published by Melanen et al. (1985) and Niinioja (1993) indicate that the use oflivestock manure can result in considerable nutrient leach- ing. The same has been suggested in many re- ports published elsewhere (e.g. Brink et al, 1979, Vetter and Steffens 1981,Brink and Jernlås 1982, Herrmann et al. 1983). Many farms produce excessive amounts of livestock manure in relation to their field area. Another reason for excessive manuring may be that farmers do not know the fertilizer value of © Agricultural Science in Finland Manuscript received January 1995 363 AGRICULTURAL SCIENCE IN FINLAND https://www.c-info.fi/en/info/?token=K8-p5rv_41EkZYrV.xr5O_SJwh6luxqOpXeQ8ew.XwLdhMko0g3C6FpoC1_apX3WoHWAcYgc6iGNjEvALUU0hdtrOz5X66MR6tLAKdbOuxQrKxL9FM9oUc50WXQKmLowS5QaeXqmNY6-31oZlYRcTUtDgw5w54nG5C5fXeeFArXRs4D7RTVlGPCp61xDRkhijgly_rC6Y0nN250DIQYKYhr35LekQAv1MQlyJBOSDU-aG3JeSTAKzcpD2mrUvXonDxZSTyNJz7wTCV-VxIB6zxMW-tD3MLoa8SfXkOYEP_AeWD3V6g Kemppainen, E.: Leaching and uptake ofnitrogen and phosphorus... manure. Owing to insufficient storage capacity, about one-third of the manure produced in Fin- land has to be spread in autumn and some in winter. One way in which the hazards of nitrogen leaching have been overcome is by preventing the oxidation of manure ammonia with nitrifi- cation inhibitors. The use of inhibitors in com- bination with manure application in autumn has given rather good results. However, the profita- bility of inhibitor use is still unclear (Amberger 1981, Kjellerup 1986, Pain et al. 1987, Görlitz et al. 1988, Kemppainen 1989a, Tveitnes and Håland 1989,Görlitz 1990, Meissner et al. 1991, Nilsson 1991). Not only manure nitrogen but also manure phosphorus poses a threat to the environment when leached from fields. The phosphorus con- tent of even a moderate dose of manure may of- ten be too high for a crop. Moreover, manure phosphorus is more mobile in soil and thus more susceptible to leaching than the phosphorus in mineral fertilizer (Vetter and Steffens 1981). German studies show that acid peat soils are susceptible to phosphorus leaching (Scheffer et al. 1981, Vetter and Steffens 1981). Very high amounts of phosphorus can leach through a peat soil when manure is applied in large quantities (Scheffer et al. 1981. Bartels and Scheffer 1987). The risk of phosphorus leaching from acid peat soils appears to have been overlooked in Fin- land even though peat fields account for over 20% of the arable area. Fur animal manure is particularly problem- atic for environmental protection, as manures of mink and fox contain about 10 times more ni- trogen and 30 times more phosphorus than cow slurry (Kemppainen 1984). Thus fur animal ma- nures often cause considerable environmental problems on farms (Pedersen 1991). Owing to the high nutrient content of the manures it is very difficult to spread them at rates low enough to avoid overfertilization. Moreover, many fur an- imal farms have too small an arable area for manure spreading. This study compares the leaching and uptake of nitrogen and phosphorus from two different manures: cow slurry and fox manure. Cow slur- ry was chosen because it is an important type of manure on Finnish farms, and fox manure be- cause of its widespread use in eastern Finland and Ostrobothnia. Only a few studies on the fer- tilizer value and environmental impacts of fox manure, a very concentrated type of manure, have been published. Two soil types typical of northernFinland were chosen for the experiment: Carex peat and fine sand. The effects of spread- ing timeand use of a nitrification inhibitorwere also studied. Material and methods A lysimeter field with 66 small lysimeters (il- lustrated in Fig. 1)was set up at the Agricultural Research Centre of Finland, Kainuu Research Station, Sotkamo (64° 06’N, 28° 20’E) in sum- mer 1989.All the lysimeters are monoliths. Half of them were filled with cultivated fine sand soil and half with Carex peat soil (Table 1). The treat- ments used in the experiment are given in Table 2. There were three replicates. Before the experiment was set up, the lysim- eter soils were all turned (ploughed) to a depth of 10 cm. Manures applied to lysimeter soils in September were harrowed into the soil about three hours after spreading. In December, the manures were spread on a 3-4-cm-thick snow cover. In May, they were first spread on lysime- ter soils which had been harrowed to a depth of 10cm and were then harrowed into the soil three days after application. The nutrient content of manures and the amounts of nutrients applied to the lysimeter soils are presented in Table 3. Ma- nure samples were analysed according to Kemp- painen (1989b). Spring barley (Hordeutn vulgare var. Arra, 500 germinating seeds/m2 ) was grown in lysim- eters during 1990-1992. During 1991 and 1992, the residual effects of different treatments were studied and the lysimeters were fertilized equal- ly with mineral fertilizer (N-P-K = 85-30- 60 kg/ha). 364 AGRICULTURAL SCIENCE IN FINLAND AGRICULTURAL SCIENCE IN FINLAND Vol. 4: 363-375. Water tanks were emptied and water samples taken from the lysimeters 13 times. The amount of water was measured by weighing. Total ni- trogen, nitrate nitrogen, ammonium nitrogen, total phosphorus and soluble phosphorus contents were analysed as required by Finnish standards (SFS 3031, SFS 3030, SFS 3032 and SFS 3026). Phosphorus was analysed on sam- Table 1. Chemical properties of experimental soils. Nutrients were determined according to Vuorinen and Mäkitie (1955), and pH from a mixture of soil/deionized water = 1/2.5. Soil type pH mg/1 Ca K Mg P Fine sand soil 6.3 1170 110 79 10,4 Carex peal soil 5.0 800 43 239 3.0 Fig. 1.Diagram of a lysimeter. 365 Kemppainen, E.: Leaching and uptake ofnitrogen and phosphorus... Table 2. Treatments of various lysimeters. Soil Manure Manure Spreading/ Spreading date type type rate treatment Carex Cow 48 t/ha September 26,09.1989 peat slurry " " + Didin (15 kg/ha) 26.09.1989 ” " " December 19,12.1989 " " " May 11.05.1990 " Fox 23 t/ha September 26.09,1989 " manure " " +Didin (15 kg/ha) 26.09.1989 " " " December 19.12.1989 " " ” May 11.05.1990 Fine Cow 48 t/ha September 26.09.1989 sand slurry " " +Didin (15 kg/ha) 26.09.1989 " " " December 19.12.1989 " " ” May 11.05.1990 " Fox 23 t/ha September 26.09.1989 manure " " +Didin (15 kg/ha) 26.09.1989 " ” " December 19.12.1989 " " " May 11.05.1990 Carex Unfert. - - - peat NPK 500 kg/ha May 14.05.1990 Fine Unfert. - - sand NPK 500 kg/ha May 14.05.1990 pies conserved withsulphuric acid. The amount of organic nitrogen was calculated by subtract- ing nitrate and ammonium nitrogen from the amount of total nitrogen. Combined results for the whole experimental period are mostly pre- sented. In some cases, however, results for short- er leaching periods are given as follows: periodi 26.09.1989-09.05.1990 period II 10.05.1990-28.05.1991 period 111 29.05.1991 - 15.05.1992 period IV 16.05.1992-05.11.1992 The majority of the lysimeters functioned well; a few had to be discarded owing to excep- tionally small runoff. The results for unfertilized peat are based on only two replicates and those for unfertilized fine sand on one lysimeter. In addition to nutrient leaching, grain and straw yields were measured and their nitrogen and phosphorus contents analysed according to Kähäri and Nissinen (1978). Nitrogen and phos- phorus uptake was calculated. The proportion of manure nitrogen and phosphorus in the yield and runoff was calculated by first subtracting the Table 3. Nutrient content of different manure types (g/kg, on fresh weight basis) and amounts of nutrients applied to lysimeter soils (kg/ha). Nutrient g/kg kg/ha Cow Fox Cow Fox NPK Total N 3.47 24.55 167 565 85 Soluble N 2.15 14.32 103 329 85 Total P 0.72 29.50 35 679 30 Total K 3.63 9.27 174 213 60 366 AGRICULTURAL SCIENCE IN FINLAND Vol. 4: 363-375. amounts of N and P in the corresponding unfer- tilized treatments. The experiment was set up and the results were calculated according to the split-split-plot design. The main plots consisted of two soil types, subplots of two manure types, and sub- subplots of four spreading treatments. In some cases, one-way variance analyses were also ap- plied. AVAX 11/780computer and SAS program were used for the calculations (SAS Institute 1985). Results Runoff Throughout the experimental period significantly more water percolated through manure-treated peat soil than through fine sand soil (Table 4), the percolation level corresponding to 49% and 24% of precipitation, respectively. Runoff was exceptionally high during the third leaching pe- riod (Fig. 2). The figure also shows that the dif- ference between soil types increased steadily as the experiment proceeded. 11.5 kg/ha). The most striking difference was in the leaching of ammonium N: about 50 times more ammonium N leached from peat soil than from fine sand soil. However, there was no sig- Table 4. Runoff from lysimeters. Averages for soil types, manure types and spreading times, and figures for control treatments. Less water percolated through lysimeter soils fertilized with fox manure than through those fertilized with cow slurry. There always seemed to be less runoff water from lysimeters manured in May than from other spreading treatments. Runoff from control treatments was of the same order as from lysimeters fertilized with livestock manure. However, there was substantially less runoff from unfertilized peat thanfrom other peat soils. Treatment Number of lysimeters Runoff, mm Peat soil 24 820 Fine sand soil HSD (P = 0.05) 24 406 322 Cow slurry Fox manure 24 671 24 555 HSD (P = 0.05) 97 September September+Didin December May HSD (P = 0,05) 12 678 12 667 12 618 12 490 Leaching of nitrogen 160 No significant interactions More ammonium N, organic N and total N leached from manure-treatedpeat soil than from fine sand soil (Table 5). One-way variance anal- ysis showed that the difference in the leaching of total N was statistically significant (HSD = Peat Unfert. 2 3 294 NPK 784 Fine Unfert. sand NPK 1 528 3 370 Fig. 2. Cumulative runoff from manure-treated peat and fine sand soil lysimeters. 367 AGRICULTURAL SCIENCE IN FINLAND Kemppainen , E.: Leaching and uptake ofnitrogen andphosphorus nificant difference in the leaching of nitrate N. The proportion of total nitrogen that was nitrate nitrogen in percolated water increased in the course of the experiment in both soil types (Fig. 3). In peat soil, the proportion of ammoni- um nitrogen remained significant towards the end of the experiment whereas in fine sand soil it was low throughout. As a whole, manure type had no effect on the leaching of the various nitrogen fractions. However, in peat soil slightly more total N, ni- trate N and ammonium N seemed to leach from fox manure than from cow slurry but in fine sand soil the situation was reversed. Leaching of all nitrogen fractions was lowest in spring-applied manure. For ammonium nitrogen, however, this was true only in peat soil. In fine sand soil, leach- ing of ammonium nitrogen was negligible (0.3- 0.4 kg/ha) at all spreading times. Didin did not decrease nitrogen leaching. Manure spreading time exerted an influence throughout the ex- perimental period (Fig. 4). Table 5. Leaching of nitrate, ammonium, organic and total nitrogen. Averages forsoil types, manure types and spreading times, and figures for control treatments. Treatment NOj-N nh4-n Org.N kg/ha Tot.N kg/hakg/ha kg/ha Peat soil 33.8 15.2 19.9 68.8 Fine sand soil HSD (P = 0,05) Cow slurry 27.9 0.3 6.9 35.2 50.1 3.8 5.6 47.9 32.5 7.1 14.2 53.9 Fox manure 29.2 8.4 12.5 50.1 HSD (P = 0.05) September 6.7 1.4 2.9 7.0 35.4 9.1 15.0 59.6 September+Didin December May 33.3 9.1 15.5 57.9 33.0 8.3 13.5 54.8 21.6 4.5 9.5 35.6 HSD (P = 0.05) 14.8 3.9 4.9 18.6 Significant interactions: - soil x manure * � * - soil x spreading Peat L ** Unfert. NPK 10.0 3.4 6.5 19.9 28.5 12.9 13.3 54.6 Fine sand Unfert. NPK 15.1 0.2 3.6 18.8 14.9 0.3 3.8 19.0 Fig. 3. Proportion of organic, ammonium and nitrate nitro- gen relative to total nitrogen in runoff from manure-treated lysimeters during various leaching periods. 368 AGRICULTURAL SCIENCE IN FINLAND Vol. 4: 363-375. Leaching of phosphorus way variance analysis demonstrated that the dif- ferences were statistically significant in 1991 (HSD = 660kg/ha) and in 1992 (HSD = 410 kg/ ha). Fox manure invariably produced higher grain yields than cow slurry. In the last experi- mental year, the advantage gained by fox ma- The amount of total phosphorus leached from manure-treated peat soil was 23 times that from fine sand soil (Table 6). The difference in the leaching of soluble phosphorus was 39-fold. According to one-way variance analysis, the differences were statistically significant (HSD for total P = 979 g/ha, for soluble P = 899 g/ha). The highest single figure, 4609 g total P/ha, was for fox manure applied to peat soil in December. Table 6. Leaching of total and soluble phosphorus. Averages for soil types, manure types and spreading times, and figures for control treatments. Treatment Total P Soluble P g/ha g/haLeaching of phosphorus from peat soil was considerably higher during the latter two leach- ing periods than during the former two (Fig. 5). The figure also shows that the differences be- tween soil and manure types increased towards the end of the experiment. More phosphorus leached from fox manure than from cow slurry (Table 6). For total phosphorus, however, this was true only in peat soil. In fine sand soil, leach- ing of total P was very low (about 100 g/ha) and not connected with manure type. No differences were found between spreading times. Peat soil 2264 1848 Fine sand soil HSD (P = 0.05) Cow slurry 99 47 2271 2102 611 437 Fox manure 1752 1457 HSD (P = 0.05) September 1098 1042 1142 897 September+Didin December May 779 570 1567 1275 1238 1048 HSD (P = 0.05) 1818 1682 Significant interactions: - soil x manure * Barley grain yields Peat Unfert. 239 106 NPK 709 520 Higher grain yields were always harvested from peat soil than from fine sand soil (Table 7). One- Fine Unfert. sand NPK 62 19 124 33 Fig. 4. Cumulative leaching of total nitrogen from unferti- lized lysimeters and from those manured in September and in May. Fig. 5. Cumulative leaching of total phosphorus from fox manure in peat soil, cow slurry in peat soil and manures in fine sand soil. 369 AGRICULTURAL SCIENCE IN FINLAND Kemppainen, E.: Leaching and uptake ofnitrogen and phosphorus. nure was rather small in peat soil but substan- tially higher in fine sand soil. In the first experimental year, spreading in December resulted in a lower yield than other treatments. Cow slurry produced the highest yield when spread in May and fox manure when spread in September with Didin. In the second year, there were no significant differences be- tween the spreading treatments but, in the third year, spring application proved to be better than spreading in September with Didin. However, whilst differences between the various treatments with cow slurry were rather small, fox manure clearly produced the best yield when spread in spring. Comparison with unfertilized lysimeters in- dicated that very high yield increases were achieved with various fertilization treatments in the first year. In the second and third experimen- tal years, there were substantial residual effects, especially with fox manure. Table 7. Barley grain yields. Averages for soil types, ma- nure types and spreading times,and figures for control treat- ments. Barley grain yield, kg/haTreatment 1990 1991 1992 Peat soil 8000 6460 4950 Fine sand soil 5750 5020 4130 HSD (P =0.05) 1800 2350 1190 Cow slurry 4720 5230 4290 Fox manure 9030 6250 4800 HSD (P =0.05) 980 1040 90 September 6990 5740 4430 September+Didin 7770 5740 4180 December 5310 5830 4630 May 7440 5660 4930 HSD (P = 0.05) 1130 1060 600 Significant interactions - soil x manure *** manure x spreading ** * Peat Unfert. 1830 5230 4090 NPK 6970 6010 6000 Fine Unfert. 1280 4390 3930 sand NPK 4680 4220 3800 Calculated proportion of manure nitrogen in yield and runoff Substantially higher amounts of manure nitro- gen were found in barley (grain+straw) from peat soil than from fine sand soil (Fig. 6). The amounts of manure nitrogen leached from peat soil were higher, too. Of applied total nitrogen, 41% was found in the yield and 13% in runoff from peat soil, and 25% in the yield and 5% in runoff from fine sand soil. Barley treated with fox manure contained much higher amounts of manure nitrogen than that fertilized with cow slurry. However, the amounts of nitrogen leached from fox manure were only similar to or lower than those leached from cow slurry. Of applied total nitrogen, 33% was found in barley and 5% in runoff from fox manure, and 34% in barley and 21% in runoff from cow slurry. Recovery of manure nitrogen in barley was highest after application in September with Di- din, and lowest after application in December (Fig. 7). Amounts of manure nitrogen leached Fig. 6. Manure nitrogen in barley yield (grain+straw) and in runoff during the whole experimental period. Compari- son of soil and manure types. 370 AGRICULTURAL SCIENCE IN FINLAND Vol. 4: 363-375. were of the same order for applications in Sep- tember, September+Didin and December, but substantially lower after application in May. Of applied total nitrogen, 33%, 40%, 24% and 34% were found in barley after manure application in September, September+Didin, December and May, respectively; the figures for nitrogen found in runoff were 11%, 11%, 10% and 4%, respec- tively. In peat soil, recovery of fertilizer nitrogen (NPK plots, 85 kg N/ha) in barley was about 150% and in runoff 41%. In fine sand soil, 46% of nitrogen applied in mineral fertilizer was re- covered in barley but none in runoff. Calculated proportion of manure phosphorus in yield and runoff Substantially higher amounts of manure phos- phorus were found in barley (grain+straw) from peat soil than from fine sand soil (Fig. 8). Sig- nificant amounts of manure phosphorus leached from peat soil but practically none from fine sand soil. Of applied total phosphorus, 11.1% was found in barley and 0.6% in runoff from peat soil; the figures for fine sand soil were 6.2% and 0%, respectively. Barley fertilized with fox manure contained much higheramounts of manure phosphorus than that fertilized with cow slurry. In peat soil, a corresponding difference was evident in the leaching of manure phosphorus. Of applied to- tal phosphorus, 50.6% was found in barley and 2.6% in runoff from cow slurry in peat soil, and 9.1% in barley and 0.5% in runoff from fox ma- nure. In fine sand soil, recovery of manure phos- phorus in barley was 19.1% for cow slurry and 5.5% for fox manure. Recovery of manure phosphorus in barley was highest after application in May and lowest after application in December (Fig. 9). The amount ofmanure phosphorus leached was high- est after application in December but the differ- ences between treatments were rather small. Of applied total phosphorus, 8.4%, 9.4%, 7.3% and 9.6% were found in barley after manure appli- cation in September, September+Didin, Decem- ber and May, respectively; the figures for phos- Fig, 7. Manure nitrogen in barley yield (grain+straw) and in runoff during the whole experimental period. Compari- son of various spreading treatments. Fig. 8. Manure phosphorus in barley yield (grain+straw) and in runoff during the whole experimental period. Com- parison of soil and manure types. 371 AGRICULTURAL SCIENCE IN FINLAND Kemppainen, E.; Leaching and uptake ofnitrogen andphosphorus... phorus found in runoff were 0.3%, 0.2%, 0.4% and 0.3%, respectively. Of applied fertilizer phosphorus (30 kg P/ha) in NPK plots, 78.8% was found in barley and 1.6% in runoff from peat soil; the figures for fine sand soil were 26.1% and 0.2%, respectively. Discussion Peat soil was superior to fine sand soil with re- spect to barley yields and nutrient uptake. How- ever, nutrient leaching from peat soil was also usually higher. As to leaching of total N and or- ganic N, the difference is explained partly by higher natural leaching and partly by high run- off from peat soil. High leaching of ammonium nitrogen from peat soil is partly due to significant mineraliza- tion of its organic nitrogen and partly to manure ammonia. Owing to the low pH, ammonia oxi- dizes slowly in peat (Alexander 1991, p. 252). Considerable leaching of ammonium nitrogen from peat soil was found by Jaakkola and Yläran- ta (1985). Substantially more phosphorus leached from peat soil than from fine sand soil, indicating the low capacity of peat soil to bind phosphorus. Marked differences in the phosphorus binding capacity of mineral soils and organogenic soils were noted by Steenvoorden and Oosterom (1981) and Uhlen and Österud (1992). Very high phosphorus losses from manure-treatedpeat soils were demonstrated by Scheffer et al. (1981) and Bartels and Scheffer (1987). A conspicuous feature of this study was the high P loss during the two latter leaching periods, especially from fox manure in peat soil. It seems that the leaching of phosphorus began about two years after manure application. It is probable that significant P losses will occur af- ter the end of the experiment and the differences between various treatments will still increase. Fox manure was superior to cow slurry with respect to barley yields and nutrient uptake. However, the leaching of total nitrogen from fox manure was more or less the same as that from cow slurry even though the amount of total ni- trogen supplied in fox manure was 3.4-fold that in cow slurry. The luxuriant vegetation produced by fox manure used much waterand thus reduced runoff more effectively than cow slurry. Ample fertilization and subsequent luxuriant growth have been shown to reduce runoff in other studies, too (Eder 1985, Jaakkola and Yläranta 1985, Stauffer and Enggist 1990). The phenomenon described above, that is, a higher nutrient amount increasing yield and nu- trient uptake but not nutrient leaching, is possi- ble only when other growth factors do not limit plant growth. Growing conditions seem to have been surprisingly good in the lysimeters because barley was able to utilize the high level of nitro- gen supplied by fox manure. However, these re- sults are not comparable to those at field scale, where leaching becomes important at substan- tially lower nitrogen levels. Nitrogen uptake by barley was highest after treatment with manure with Didin in September, Fig. 9. Manure phosphorus in barley yield (grain+straw) and in runoff during the whole experimental period. Com- parison of various spreading treatments. 372 AGRICULTURAL SCIENCE IN FINLAND Vol. 4: 363-375. and lowest in treatment with manure in Decem- ber. The differences between treatments are mainly ascribed to differences in ammonia vol- atilization. December was an especially poor spreading timebecause manure could not be in- corporated into the frozen soil. September was better because the manure was mixed into the soil shortly after spreading. In May, manure was left on the soil surface for three days before it was incorporated; the delay is reflected in the N recovery data. The effect of spreading time on the leaching of total nitrogen, nitrate nitrogen, ammonium nitrogen and organic nitrogen was clearly dem- onstrated. Nitrogen leaching was lowest from the spring application and highest from applications in September. These results are consistent with those reported in the literature (Vetter and Stef- fens 1977, 1981, Brink and Jernlås 1982, Dijk 1985, Ivarsson and Brink 1985, Brink 1987, Görlitz 1989,Torstensson 1992). Didin did not have a significant effect on ni- trogen leaching. This was surprising because several studies have shown that Didin is very effective at saving nitrogen (Amberger 1989, Asmus 1989, Görlitz 1989). According to Am- berger (1991) Didin may have a poor effect if manure is spread too early. Didin then decom- poses and loses its effect before winter. In this study, slurry treated with Didin was spread in late September, which, according to Kemppai- nen (1989a), is usually a favourable time for Di- din in Finland. However, after application, the soil temperature fell from about 11°C to 3-4°C in a few days and remained very low until the soil froze at the end ofNovember. It is thus very unlikely thatDidin lost its effect through decom- position during the autumn. It is more likely that the soil temperature was too low for significant nitrification to occur and so Didin had only a minor effect (Maidl and Fischbeck 1989). This conclusion is supported by the fact that, during the first leaching period, leaching of nitrate ni- trogen from lysimeters fertilized with livestock manure was rather small. Didin, however, seemed to have a positive effect on the recovery of nitrogen in barley. 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SELOSTUS Naudan lietelannan ja ketun lannan typen ja fosforin huuhtoutuminen ja hyväksikäyttö lysimetrikokeessa Erkki Kemppainen Maatalouden tutkimuskeskus Lysimetrikokeessa tutkittiin maalajin, levitysajan sekä nitrifikaatioinhibiittorin (Didin) vaikutusta nau- dan lietelannan ja ketun lannan typen ja fosforin huuhtoutumiseen ja hyväksikäyttöön. Valunta ja kokonaistypen huuhtoutuminen turve- maasta olivat noin kaksinkertaisia hietaan verrattu- na. Nitraattitypen huuhtoutumisessa ei ollut merkit- tävää eroa, mutta amraoniumtyppeä turvemaasta huuhtoutui 50-kertainen määrä ja orgaanista typpeä 3-kertainen määrä hietaan verrattuna. Turvemaasta huuhtoutui myös kokonaisfosforia 23-kertainen mää- rä ja liukoista fosforia 39-kertainen määrä hietaan verrattuna. Naudan lietelannasta ja ketun lannasta huuhtou- tui suunnilleen yhtä paljon typpeä. Typpeä huuhtou- tui vähiten toukokuussa levitetystä lannasta. Didin ei vähentänyt typen huuhtoutumista merkitsevästi. Ke- tun lannasta huuhtoutui turvemaalla selvästi enem- män fosforia kuin naudan lietelannasta. Lannan le- vitysaika ei vaikuttanut fosforin huuhtoutumiseen. Turvemaa tuotti huomattavasti suurempia ohrasa- toja kuin hieta. Ketun lanta oli selvästi naudan liete- lantaa tehokkaampaa lannoitetta. Ensimmäisenä koe- vuonna lannan levitys joulukuussa vähensi merkitse- västi sen lannoitusvaikutusta muihin levitysaikoihin verrattuna. Ohran jyvä- ja olkisadon perusteella las- kettuna lannan kokonaistypen näennäinen hyväksi- käyttö oli 24-41 % ja lannan fosforin hyväksikäyttö 6-51 %. Lannan kokonaistypestä huuhtoutui 4-21 % ja fosforista 0-2,6 %. 375 AGRICULTURAL SCIENCE IN FINLAND