Voi 611997): 295-303. Effects of variety, soil type and nitrogen fertilizer supply on the nutritive value of barley for growing pigs Jarmo Valaja Agricultural Research Centre ofFinland, Swine Research Station, Tervamäentie 179, FIN-05840 Hyvinkää, Finland. Current address: Agricultural Research Centre ofFinland, Institute ofAnimalProduction, FIN-31600 Jokioinen, Finland, e-mail: Jarmo, valaja @mtt.fi Kaija Suomi, Timo Alaviuhkola Agricultural Research Centre ofFinland, Swine Research Station, Tervamäentie 179, FIN-05840 Hyvinkää, Finland Timo Mela Agricultural Research Centre ofFinland. Institute ofCrop and Soil Science, FIN-31600, Jokioinen, Finland The effects of variety, soil type and nitrogen (N) fertilizer supply on the nutritive value of barley were studied with chemical analysis, in vitro digestibility and a growth experiment on 240 growing/ finishing pigs (live-weight 25-95 kg). Twelve barley batches were formed from three varieties (two- rowed Kustaa and six-rowed Arra and Pokko) grown on mould or clay soil and fertilized with either a low or normal level of N (43 or 71 kg N/ha to mould soil and 76.5 or 110 kg N/ha to clay soil). In the growth experiment all the diets contained similar amounts of barley and soya bean meal (845/120 g/kg). Variety Arra contained 21 g/kg more CP than did Pokko or Kustaa. The N fertilizer supply slightly increased the CP content of the barley samples (133 vs. 141 g/kg) but decreased the amount of lysine in the protein (31 vs. 29 g/ 160g N). Regression equation showed that CP and (i-glucan were positive- ly and neutral detergen fibre content negatively related to the in vitro digestibility of N in barley samples. The content of CP and the in vitro digestibilities of dry matter and N were highest in var. Arra. The daily gain and feed conversion ratio (FCR) of the pigs on Arra-based diets was better than that of those on Kustaa or Pokko (P<0.05). A higher N fertilizer supply increased slightly the CP content of barley and improved the FCR and carcass quality of the pigs (P<0.05). Soil type had only minor effects on the nutritive value ofbarley. It is concluded that, of the factors studied, variety has the greatest effect on the nutritive value of barley. Key words: daily weight gain, dry matter, feed conversion ratio, fibre, in vitro digestibility, protein © Agricultural and Food Science in Finland Manuscript received January 1997 295 AGRICULTURAL AND FOOD SCIENCE IN FINLAND https://www.c-info.fi/en/info/?token=-lKRZZIobRjXnNoZ._p2HWeho1ErrtSEwZ1OOcQ._Oy7GYxEYDyMjrQxbepRLjGPXljgdbeBl6CprbvshBb_JU49tVWo2abKQ1jcvynBPvt2f7JSvVap1kxfklYPoQWlq5ubMx3K5nbFIcrzELZb1b9r2Z8Uiveyd-AmAoduCiqv9j4FJEvCKNVVQh2MmzNMG7x7ABU8euN4wwIAhr3aaoy3kSP7zADfjlMbKT3j5wSl7urI1SU4lPPiWNdeRhjI-xgFxYzckSsxf6qHADnzSxb87vQ4wR5Dahk-tbWwV9wtRqp5iyUS_ywxU9Ae96iHRdJ2u29q_AG3IfbgX-sM5Y1BhxRmo5Inm0M Valaja, J. et al. Effects ofvariety, soil type andfertilizer supply on the nutritive value ofbarley Introduction Barley is the most commonly grown cereal crop in Finland and is mainly used as pig feed. It pro- vides most of the energy and also about half of the protein in pig diets. However, the quality of barley protein is not optimal for pigs since the content ofessential amino acids, lysine and thre- onine in particular, is very low. The main com- ponent affecting the utilization of the nutrients in barley is fibre (Taverner and Farrell 1981, Jacyno 1995). An increased fibre content lowers the energy content and can also impair the di- gestibility of protein and amino acids (Taverner and Farrell 1981, Bach Knudsen and Eggum 1984). Many factors, most notably climate, soil, variety and fertilizer, can influence the grain yield, composition and nutritive value of bar- ley. Earlier experiments revealed differences between barley varieties in digestibility and utilization by pigs (Just et al. 1983a, b). Fuller et al. (1989) found higher apparent ileal ami- no acid digestibilities in high protein than in low protein barley varieties. Nitrogen (N) fer- tilizer has increased the crude protein content of grain but lowered the lysine content in the protein (Thomke 1970, 1976). In some experi- ments soil type and cultivation location have also caused variations in the nutritive value of barley (Just et al. 1983b, Thomke 1972). The effects of all three factors on the nutritive val- ue have, however, only rarely been studied in the same experiment (Just et al. 1983a, b). Knowledge of differences in the protein and energy value of barley may improve the accu- racy of diet formulation and possibly also af- fect the amount of supplementary protein con- centrates needed in pig diets. The objective of the present experiment was to investigate the effects of variety, N fertilizer supply and soil type on the nutritive value of barley for growing pigs. The nutritive value of barley samples was assessed by their in vitro di- gestibility and the growth performance of the pigs. Some observations of the utilization of the protein by pigs related to N intake were also made. Material and methods Barley batches Three barley varieties, the two-rowed variety Kustaa and the six-rowed varietiesArra and Rok- ko, were chosen for the experiment. They were grown on either mould or clay soil. N fertilizer was applied at two levels to both soil types; 43 or 71 kg N/ha to mould soil and 76.5 or 110 kg N/ha to clay soil, to represent a low or normal level of N supply for each soil type. Twelve bar- ley batches were produced at the Institute ofCrop and Soil Science of the Agricultural Research Centre in Jokioinen, southern Finland (60°49’N and 23°30’E), in growing season 1990. Animals, diets and experimental procedure A performance trial was conducted on 240 York- shire and Landrace pigs. The pigs, with an aver- age weight of 25.0 kg (SE 0.33), were assigned by sex, weight and litter origin to one of 12 treat- ments based on the 12barley batches. Two pigs were placed in each pen, gilts and castrates be- ing kept apart. Each diet was tested on five pens ofgilts and five pens of castrates. The treatments were arranged 3X2X2 factorially to test the effects of variety, N fertilizer supply and soil type. Each barley (845 g/kg) was supplement- ed with a constant level of soya bean meal (120 g/kg) and mineral and vitamin mixture (35 g/kg).The barleys were ground in a hammer mill to pass a 3.5- mm mesh. The protein and amino acid supply of the pigs varied owing to the variation in the protein and amino acid con- tent of the barley batches. The digestible crude protein (CP) and lysine contents in the diets 296 AGRICULTURAL AND FOOD SCIENCE IN FINLAND Vol. 6 (1997): 295-303. ranged from 121 to 144 g/kg and from 5.2 to 5.5 g/kg, respectively. The protein and lysine contents in the diets were kept below the level recommended in the nutritional requirements (Salo et al. 1990) in order to maximizethe amount of barley in the diets and to reveal differences in the quality of protein in barley for pigs. The pigs were housed in partially slatted, concrete-floored pens providing free access to water. They were weighed at 2-week intervals, and their feed consumption was determined dai- ly pen by pen. The pigs were fed twice a day on a restricted scale in relation to age (1.4 to 2.9 kg/ pig/day); the daily allowance was increased by 0.2 kg/week at the beginning, and by 0.1 kg/week after the eighth experimental week. Before feed- ing the diets were mixed with water. The pigs were slaughtered when they reached a weight of 95 kg, and their carcass weight was recorded 24 h after slaughter. The carcass quality of the pigs was determined as described by Valaja et al. (1993). Chemical analyses A standard feed analysis was conducted on the barley samples and soya bean meal (AOAC 1984). The dry matter (DM) of the feeds and in vitro residues was determined after 24 h at 105°C. The DM content of the diets was deter- mined at 2-week intervals. The CP content of the feeds and in vitro residues were analysed as 6.25 * Kjeldahl N. Ether extract (EE) was ana- lysed after acid hydrolysis. The amino acids of the barley samples and soya bean meal were de- termined by gas chromatography after hydroly- sis with 6 N HCI at 110°C for 20 h (Näsi and Huida 1982). The barley samples were also ana- lysed for neutral detergent fibre (NDF) content by the method of Robertson and Van Soest (1981), for total dietary fibre content by the method of Asp et al. (1983) and for (3-glucans content by the method of McClear and Glennie- Holmes (1985). In vitro assay was performed on the barley samples by the method ofBoisen and Fernandez (1991), a two-step procedure compris- ing a 6-hour incubation in pepsine solution fol- lowed by an 18-hour incubation in pancreatin solution. Instead ofcontinuous magnetic stirring, handmixing every half-hour was used during the first incubation step. Calculations and statistical analysis The net energy content and feed unit values of the barley samples were calculated with theFinn- ish energy evaluation system for pigs (Tuori et al. 1995) using constant digestibility coefficients. The data were analysed by the GLM procedure of SAS (1985). The four-way analysis of vari- ance model used to analyse the data was: Y..., =ii+V. + S. +N.+ SE + VS, +VN,ijklmn • j k I m jk jl + SN kl +e, where Y.jk|mn is the dependent variable, pis the overall mean, V. is the effect of variety, S. is the effect of soil type and Nk is the effect of N fertilizer; VS.., VN, and SR, are the interactions’ jk’ jl kl between the factors; and SE is the effect ofsex.m e is a normal distributed random variable. The N fertilizer supply was pooled to two levels, low and normal, for the statistical analysis as it was assumed that the total available N from soil and fertilizer was approximately the same in both soils. The difference between variety means was tested with Tukey’s test. The relations between in vitro digestibilities and chemical constituents were calculated by linear regression equations. In all analyses the data on one pen were treated as an experimental unit. Results Chemical composition and in vitro digestibility The CP and lysine contents of the barley batches ranged from 120to 161 g/kg DM and from 27 to 297 AGRICULTURAL AND FOOD SCIENCE IN FINLAND Valaja, J. et al. Ejfects of variety, soil type andfertilizer supply on the nutritive value ofbarley 35 g/160 g N, respectively (Table 1). Arra con- tained, on average, 21 g/kg more CP than did Pokko or Kustaa. The lysine content in the pro- tein was fairly similar in all varieties, whereas the lysine content in DM was highest in Arra (4.0, 4.3 and 4.0 g/kg DM in Kustaa, Arra and Pokko, respectively). Pokko contained slightly less P- glucans than did Kustaa or Arra. The N fertiliz- er supply slightly increased the CP content of the barley samples (133 vs. 141 g/kg) but con- comitantly decreased the amount of lysine in the protein (31 vs. 29 g/!6og N). The in vitro digestibility of DM and N in bar- ley batches ranged from 65.9% to 71.9% and from 85.1 % to 88.6%, respectively, the variation being higher in DM digestibility. The mean di- gestibility of DM in Kustaa, Arra and Pokko was 67.3%, 71.0% and 69.8%, respectively, being highest in Arra. The mean digestibility of N in Kustaa, Arra and Pokko was 87.3%, 88.1% and 85.9%, being also highest in Arra (Table 1). The following regression equation was calculated for the in vitro digestibility of N (Y) as dependent and the CP, NDF and (i-glucan contents as inde- pendent variables: Y = 86.38 (SE 3.784) + 0.49 (SE 0.093) X CP -0.40 (SE 0.117) X NDF +0.76 (SE 0.332) X p-glucan (P<0.001; r2 =0.91). No such equation could be calculated for the in vitro digestibility of DM. Performance and carcass quality of the pigs The performance results are presented as the main factors in Tables 2 and 3 since the interac- Table 1. Chemical composition of barley batches (g/kg DM) Variety Kustaa Aira Pokko Soil type Mould Clay Mould Clay Mould Clay N supply, kg/ha 43 71 76.5 110 43 71 76.5 110 43 71 76.5 110 Dry matter, g/kg 881 884 903 858 882 886 883 885 883 885 906 894 Crude protein 134 137 122 127 148 149 147 161 127 140 120 132 Etherextract 13 18 17 18 22 18 23 25 21 22 21 22 Crude fibre 55 54 54 53 58 51 49 51 53 58 53 52 Nitrogen free extract 777 769 782 777 749 759 753 737 776 757 782 769 Ash 22 21 25 24 23 23 27 26 22 23 24 25 NDF 212 220 225 235 237 217 207 227 237 241 233 231 Total dietary fibre 212 225 234 235 244 233 226 209 226 221 224 224 (3-glucans 42 43 48 43 42 44 42 43 35 38 36 37 Amino acids, g/160 g N Lysine 32 28 31 32 31 29 29 27 35 28 31 31 Threonine 27 28 31 31 28 30 31 29 33 33 33 32 Methionine 12 11 14 14 13 14 12 12 12 15 13 13 Histidine 19 21 31 24 20 18 22 25 24 20 27 30 Leucine 53 53 53 55 54 54 55 54 55 55 57 57 Isoleucine 25 25 28 27 25 26 26 25 25 26 27 27 Phenylalanine 41 40 41 42 37 39 40 41 40 40 39 40 Arginine 40 41 44 43 41 44 43 41 46 45 44 45 Valine 37 37 38 39 37 37 38 36 37 38 40 39 hi vitro digestibility, % DM 68.1 68.8 65.9 66.6 70.4 70.8 71.9 71.1 69.1 70.6 68.9 70.8 N 87.7 87.9 86.7 86.8 87.6 88.1 88.6 88.0 85.7 86.5 85.1 86.5 NDF=neutral detergent fibre. V=variety, S=soil type, N=nitrogen supply. 298 AGRICULTURAL AND FOOD SCIENCE IN FINLAND Vol. 6 (1997): 295-303. tions were insignificant. The pigs on diets com- posed ofArra grewfaster than did those on diets composed of Kustaa (P<0.05); the growth rate of pigs on diets composed of Pokko was inter- mediate. The pigs on Arra-based diets consumed less feed as DM than did the pigs on Kustaa- based diets (P<0.05). However, the N intake of the pigs on Arra-based diets was the highest among the varieties (P<0.05). The feed conver- sion ratio (FCR) of the pigs on diets composed of Arra was better than that of the pigs on diets composed ofKustaa or Pokko (P<0.05). A high- er N fertilizer supply ofbarley improved theFCR of the pigs (P<0.01), but also increased their N intake (P<0.01). The pigs fed barley grown on mould had a better FCR than did those fed bar- ley grown on clay soil (P<0.05). The carcass lean content of the pigs on Arra- based diets was higher than that of the pigs on Kustaa-based diets (P<0.05) and intermediate for the pigs on Pokko-based diets. The N fertilizer supply of barley increased the content of car- cass lean (P<0.05) and lean in valuable parts (P<0.05). The castrated male pigs gained weight faster (P<0.001) and had a better FCR (PcO.OOl) than did the gilts. In contrast, the gilts produced leaner carcasses than did the castrated males. The dif- ferences were highly significant in all carcass quality measurements. Discussion Chemical composition N fertilizer supply increased the CP content of the barley samples and decreased the content of lysine in the protein. A similar trend in CP and lysine contents was observed in the experiments Table 2. Effect of barley variety and sex on pig performance. LS means of factors are presented. Variety SEM Signif. Sex SEM Signif. Kustaa Aira Pokko n=4o Gilts Castr. n=6o males Initial weight, kg 25.0 25.0 25.0 0.17 NS 25.0 25.1 0.13 NS Final weight, kg 96.7 96.6 96.4 0.23 NS 96.7 96.4 0.19 NS Final weight, corr.kg 96.7 96.6 96.4 0.10 NS 97.0 96.1 0.33 o Carcass weight, kg 71.0 70.9 70.7 0.30 NS 71.2 70.5 0.24 o Loss at slaughter, % 26.6 26.6 26.6 0.20 NS 26.4 26.8 0.17 * Daily gain, g/day 820” 841b 827* 6.14 * 812 846 5.0 *** Days in exp. 88.1 85,8 86.8 0.76 o 89.6 84.3 0.62 *** Feed consumption, kg DM/animal 185.7” 178.2b 182.2* 1.50 ** 186.0 178.0 1.22 Nitrogen kg/animal 5.09” 5.39b 4.99” 0.042 *** 5,28 5.04 0.034 *** FCR, kg DM/kg gain 2.59“ 2.49b 2.56’ 0.019 *** 2.58 2.51 0.015 *** FU/kg gain 2.84" 2.72b 2.82” 0.021 *** 2.84 2.75 0.017 *** MJ NE/kg gain 26.4* 25.3 b 26.2” 0.19 *�* 26.4 25.6 0.16 *** Back fat thickness, mm 24.5 24.0 23.9 0.34 NS 23.1 25.6 0.28 *** Lean in valuable cuts, % 79.7 80.4 80.2 0.24 o 81,1 79.1 0.20 *** Carcass lean, % 53.0” 53.7 b 53.5* 0.20 * 54.2 52.7 0.16 *** Significance:NS=non-significant, o=P