Received for publication: 29 August, 2010. Accepted for publication: 29 June, 2012. 1 Food Engineering Program. Faculty of Agricultural Sciences. Universidad de Córdoba. Monteria (Colombia). guillermo.arrazola@ua.es 2 Department of Plant Breeding, Centre of Edafology and Applied Biology of the Segura-Consejo Superior de Investigaciones Científicas (CEBAS-CSIC). Murcia (Spain). 3 Departamento of Analytical Chemistry, Nutrition and Food Science, Faculty of Sciences, University of Alicante. Alicante (Spain). Agronomía Colombiana 30(2), 260-265, 2012 Content of the cyanogenic glucoside amygdalin in almond seeds related to the bitterness genotype Contenido del glucósido cianogénico amigdalina en semillas de almendra con relación al genotipo con sabor amargo Guillermo Arrázola1, Raquel Sánchez P.2, Federico Dicenta2, and Nuria Grané3 ABSTRACT RESUMEN Almond kernels can be sweet, slightly bitter or bitter. Bitterness in almond (Prunus dulcis Mill.) and other Prunus species is related to the content of the cyanogenic diglucoside amygdalin. When an almond containing amygdalin is chopped, glucose, benzaldehyde (bitter flavor) and hydrogen cyanide (which is toxic) are released. This two-year-study with 29 different almond cultivars for bitterness was carried out in order to relate the concentration of amygdalin in the kernel with the phenotype (sweet, slightly bitter or bitter) and the genotype (homozygous: sweet or bitter or heterozygous: sweet or slightly bitter) with an easy analytical test. Results showed that there was a clear difference in the amount of amygdalin between bit- ter and non-bitter cultivars. However, the content of amygdalin did not differentiate the other genotypes, since similar amounts of amygdalin can be found in the two different genotypes with the same phenotype. Las semillas de almendras pueden ser dulces, ligeramente ama- rgas y amargas. El amargor en almendro (Prunus dulcis Mill.) y en otras especies de Prunus se relaciona con el contenido de la amígdalina diglucósido cianogénico. Cuando una almendra que contiene amigdalina se tritura, produce glucosa, benzal- dehído (sabor amargo) y ácido cianihídrico (que es tóxico). El estudio es realizado durante dos años, con 29 variedades de al- mendra diferentes para la amargura o amargor, se ha realizado con el fin de relacionar la concentración de la amígdalina en el núcleo con el fenotipo (dulce, ligeramente amargo y amargo) y el genotipo (homocigota: dulce o amargo o heterocigótico: dulce o amarga un poco) por un ensayo de análisis fácil. Los resultados mostraron que existía una clara diferencia en la cantidad de amigdalina entre amargo / cultivares no amargo. Sin embargo, el contenido de amigdalina no diferenciaba entre los otros genotipos, ya que cantidades similares de amigdalina se puede encontrar en los dos genotipos diferentes con el mismo fenotipo. Key words: kernel taste, benzaldehyde, hydrogen cyanide, Prunus. Palabras clave: sabor amargo, benzaldehído, ácido cianhídrico, Prunus. Introduction Prunus kernels accumulate cyanogenic diglucoside amyg- dalin, which is related to bitterness (McCarty et al., 1952; Conn, 1980; Frehner et al., 1990; Møller and Seigler, 1991; Swain et al., 1992; Poulton and Li, 1994; Dicenta et al., 2002; Franks et al., 2008; Sánchez-Pérez et al., 2008). Upon disruption of tissue containing amygdalin this will be degraded by b-glucosidases with release of glucose, benz- aldehyde and hydrogen cyanide (Morant et al., 2008). This two-component system, each of them chemically inert, will provide the plant with a chemical defense against herbi- vores, insects and pathogens (Conn, 1969; Nahrstedt, 1985; Jones, 1998; Morant et al., 2003; Zagrobelny et al., 2004; Nielsen et al., 2006; Zagrobelny et al., 2007a; Zagrobelny et al., 2007b), as a source of energy reserves. In the almond (Prunus dulcis Mill.), bitterness is controlled by a single gene, Sk (Sweet kernel gene), which is domi- nant over the bitter gene (Heppner, 1923; Heppner, 1926; Grasselly and L’amandier, 1972; Kester et al., 1977; Gharbi, 1981; Vargas and Romero, 1988; Dicenta and García, 1993; Dicenta et al., 2007). The Sk gene has been localized in linkage group five in different genetic linkage maps, but its function is still unknown. As a result of the development of genetic linkage maps, some molecular markers have been found close to the Sk locus (Joobeur et al., 1998; Bliss et al., 2002; Sánchez-Pérez et al., 2007). However, a marker allele associated with the bitterness allele remains to be identified. Recently, amygdalin and, its precursor, prunasin (cyano- genic monoglucoside) content, and the enzymes involved in the amygdalin pathway, have been studied in four 261Arrázola, Sánchez P., Dicenta, and Grané: Content of the cyanogenic glucoside amygdalin in almond seeds related to the bitterness genotype different almond genotypes (Sánchez-Pérez et al., 2008). Distinct cellular localizations of the enzymes involved in the degradation pathway, possibly involving a seed coat prunasin hydrolase, have also been suggested to be related to bitterness in the almond (Sánchez-Pérez et al., 2008). Furthermore, an enzyme involved in the biosynthetic pathway has also been related to bitterness in the almond (Franks et al., 2008). On the other hand, most of the cultivated almond culti- vars are heterozygous for this trait, mainly sweet, although some of them are slightly bitter. When two heterozygous cultivars are crossed in breeding programs, 25% of the offspring will be bitter and therefore eliminated from the breeding process, since the industry is mainly interested in sweet-kernelled cultivars. This disadvantage reduces the efficiency of breeding programs, and it is especially frequent when a self-compatible progenitor is used since most of the self-compatible cultivars are heterozygous for bitterness (Grasselly and Crossa-Raynaud, 1980; Dicenta, 1991). This is indeed happening in the American Breeding Program, since the bitter P. webbi has been used as a self- compatible progenitor. Consequently, 50% of the offspring will be bitter when the other progenitor used in the cross is heterozygous, such as Carmel or Livingstone (Ledbet- ter and Pyntea, 2000; Gradziel et al., 2001). Fortunately, a study of these descendants has enabled the breeders to classify the cultivars used as progenitors as homozygous or heterozygous (Grasselly and Crossa-Raynaud, 1980; Dicenta, 1991; Vargas et al., 2001), which is a very arduous (3-4 years), and therefore expensive, task. The main objective of this research is to study the variability of the amygdalin content in a representative set of almond cultivars, its relation with the phenotype (sweet, bitter or slightly bitter) and the genotype (sweet homozygous, bit- ter homozygous or heterozygous), in order to identify the bitterness genotype of an almond cultivar with an easy analytical test. Material and methods Plant material The content of amygdalin was analyzed for two years in 23 cultivars and in six descendants of the cross Garrigues × Tuono, provided by the Almond Breeding Program of the Centre of Edafology and Applied Biology of the Segura- Consejo Superior de Investigaciones Científicas (CEBAS- CSIC) CEBAS-CSIC, Murcia (Spain). The phenotype and genotype of some of these cultivars were previously determined by different authors (Grasselly and Crossa-Raynaud, 1980; Dicenta, 1991; Vargas et al., 2001): • Sk/Sk (sweet): Del Cid, Ferragnès, Peraleja, Primorskii, Ramillete, Titan. • Sk/sk (sweet): Marcona, Desmayo Largueta, Atocha. • Sk/sk (slightly bitter): Garrigues, Genco, Tuono. • sk/sk (bitter): S3060, S3062, S3076, S3108, S3112, S3126. • Sk/Sk or Sk/sk (genotype unknown, but sweet): Achaak, Bonita, Carretas, CEBAS, Colorada, Ferraduel, La Mona, Pajarera, Planeta, Rumbeta, and Tioga. Fifty mature almond fruits were collected per cultivar and the hull and shell removed. Kernels were frozen, lyophilized in a Cryodos Telstar lyophilizer for 48h at -85°C at 1 Pa, ground, homogenized, and analyzed. Two independent analyses per sample were performed. Amygdalin content Chromatographic determination of amygdalin levels was carried out for 0.2 g of kernel samples and extracted with 10 mL of methanol for 12 h at room temperature. Kernel extractions were done with 0.1 g of polyvinyl- polypirrolidone (Sigma) or active carbon (Norit CNR 115) to ensure the freedom from pigments, which can interfere with the chromatography. Determination was performed isocratically in a waters high-performance liquid chromatography, Controler model 600 (HPLC) system, following a procedure similar already described (Kajiwara et al., 1983), under the following conditions: Waters Symmetry column 250 x 4.6 mm, flow rate 1.5 mL min-1, acetonitrile:water 20:80 as eluent, 20 µL of sample, and detection under UV at 218 nm. Results, expressed as mg amygdalin/100 g of dry weight (DW), were tested using analysis of variance and Tukey ś test to determine differences between years and cultivars. Results and discussion Analysis of variance of the amygdalin content showed sig- nificant differences between cultivars and between years as well as the year x cultivar interaction (Tab.1). For the majority of the cultivars (Ramillete, Bonita, Mar- cona etc.), the content of amygdalin was similar in both years (Tab. 2). However, in the first year the content of some cultivars was slightly lower (Achaak, Colorada, Carretas, 262 Agron. Colomb. 30(2) 2012 Ferraduel, S3060, S3076, etc.), or slightly higher (Desmayo Largueta, Garrigues, S3126, S3062, and S3112). In general, there was a high variability of the amygdalin content between the studied cultivars. The average content of the bitter cultivars was much higher than the non-bitter ones (Tab. 2). Tukey’s test with all the cultivars showed clear differences between bitter and non-bitter (sweet and slightly bitter) cultivars, but did not between or within sweet and slightly bitter ones. Within the bitter cultivars, four different groups were identified. In general, the kernel taste of cultivars is related to amyg- dalin content, although with some variability. In sweet cultivars such as Primorskii, Ramillete, Bonita and CEBAS, amygdalin was not detected, but in others such as Ferraduel or Pajarera it was until 27 mg/100 g DW. In slightly bitter cultivars such as Garrigues or Tuono, amygdalin content TABlE 1. Amygdalin content media values (X) obtained for each cultivar, in two different years and the Tukey test including all the cultivars and subtracting the bitter ones. Tukey test Means (mg/100 g muestra) All Non-bitter Flavor Genotype Cultivar Year 1 Year 2 X SE Year 1 Year 2 X Year 1 Year 2 X Sweet SkSk Primorskii nd nd nd e e e m j j Ramillete nd nd nd e e e m j j Titan 0.43 0.62 0.53 0.021 e e e m ij ij Del Cid - 2.15 2.15 0.007 - e e - hi hi Peraleja 1.76 2.56 2.16 0.021 e e e l h hi Ferragnès 5.16 5.5 5.33 0.007 e e e h fg f Sksk Marcona 1.87 1.87 1.87 0.014 e e e kl hi hij Desmayo 8.11 6.86 7.49 0.064 e e e f ef e Atocha 7.28 8.02 7.65 0.007 e e e g e e Sk-- Bonita nd nd nd e e e m j j CEBAS nd nd nd e e e m j j Tioga 0.34 0.52 0.43 0.007 e e e m ij ij Colorada 2.41 2.72 2.57 0.021 e e e jk h gh Carretas 2.49 2.65 2.57 0.014 e e e j h gh Planeta 3.71 4.65 4.18 0.007 e e e i g fg La Mona 4.63 6.1 5.37 0.007 e e e h fg f Rumbeta - 5.39 5.39 0.014 - e e - fg f Achaak 10.22 11.25 10.74 0.028 e e e e d d Ferraduel 21.96 23.36 22.66 0.021 e e e c b b Pajarera - 27.26 27.26 0.028 - e e - a a S.Bitter Sksk Genco 17.34 18.74 18.04 0.042 e e e d c c Garrigues 23.81 22.93 23.37 0.014 e e e b b b Tuono 25.05 25.81 25.43 0.014 e e e a a a Bitter sksk S3126 2.439 2.36 2.4 0.141 d d d - - - S3108 3.799 3.81 3.805 0.004 c c c - - - S3062 3.87 3.784 3.827 0.001 c c c - - - S3060 4.915 5.036 4.976 0.003 b b b - - - S3112 5.206 5.011 5.109 0.006 b b b - - - S3076 5.894 6.028 5.961 0.043 a a a - - - TABlE 2. ANOVA of amygdalin content among varieties and years (1997, 1998), for the studied cultivars. SV Sum of squares DF Mean square F ratio Significance F Main effects 571790283 29 19716906 18779.41 < 0.001 Year 9362 1 9362 8.92 0.004 Variety 571786321 28 20420990 19447.97 < 0.001 Year x variety 107439 22 4883 4.65 < 0.001 Explained 571897721 51 11213681 10680.00 < 0.001 Residual 112342 107 1050 Total 572010063 158 3620317 263Arrázola, Sánchez P., Dicenta, and Grané: Content of the cyanogenic glucoside amygdalin in almond seeds related to the bitterness genotype was until 25 mg/100 g DW. In bitter cultivars, the detected levels were between 2400 mg/100 g DW (S3126) and almost 6000 mg/100 g DW (S3076). The number of plant species with cyanogenic glucosides is more than 2650 (Seigler and Brinker, 1993; Bak et al., 2006). Studies on passion fruit seeds (Passiflorae) obtained 3.1 mg/100 g DW (Chassagne et al., 1996), in lime 7000 mg/100 g DW of linamarin, and in bamboo 500 mg/100 g DW of taxiphyllin (Shahidi and Wanasundara, 1997). Furthermore, in linen seeds, linamarin (300 mg/100 g DW) and linustatin (6000 mg/100 g DW) were detected. Amygdalin has also been detected in other fruit tree kernels such as plum (4,100 mg/100 g), apricot (2,394 mg/100 g), cherry (2,306 mg/100 g) and apple (739 mg/100 g) (Lucas and Sotelo, 1983). In the case of apricot, different concen- trations of amygdalin have been detected: 4,400 and 6,500 mg/100 g DW (Femenia et al., 1995), or 5,500 and 7,000 mg/100 g DW (Stoewsand et al., 1975; Briggs and Yuen, 1978; Mandenius et al., 1983; Stosic et al., 1987; Voldrich et al., 1989; Gómez et al., 1998; Negri et al., 2008). In bitter almond kernels, amygdalin levels were 5,808 mg/100 g DW (Corradi and Micheli, 1982; Shahidi and Wanasundara, 1997). Another analysis determined 926 mg of amygdalin/100 g DW for the bitter variety Sassari 11 and 106 mg/100 g DW in the sweet variety Arrubia, but in the sweet variety Texas, amygdalin was not detected (Usai and D’hallewin, 1992). Amygdalin content in sweet cultivars and slightly bitter ones from Sicily and Apulia was higher than the American or Russian ones, except for the American variety Perlees (Barbera et al., 1987). In fact, the content detected by these authors was much higher than the content detected in this study for cultivars such as Genco (390 versus 18 mg/100 g) and Tuono (750 versus 25 mg/100 g). Recently, 9 µmol/100 mg fresh weight (FW) of amygdalin (4,082 mg amygdalin/100 g FW) was detected in the bitter genotype S3067 (Sánchez-Pérez et al., 2008). In general, the differences in amygdalin levels found in bit- ter cultivars of different Prunus species are within the range determined in this study (2,400-6,000 mg/100 g), with the exception of the results already mentioned (Barbera et al., 1987; Shahidi and Wanasundara, 1997). In order to detect some differences within the non-bitter group, we analyzed the data without the bitter cultivars, since their amygdalin content was much higher. Analysis of variance only with non-bitter cultivars showed significant differences between cultivars and between years, the same as when all the genotypes were included (Tab. 3). The Tukey’s test did not differentiate sweet and slightly bitter cultivars (Tab. 2). In general, sweet cultivars had less amygdalin than the slightly bitter ones, although some sweet cultivars (Ferraduel and Pajarera) had similar or higher amygdalin content than some slightly bitter ones. Tukey’s test neither distinguised between homozygous and heterozygous sweet cultivars. In general, sweet homozygous cultivars (Sk/Sk) contained lower levels of amygdalin than the sweet heterozygous ones, but Marcona (Sk/sk) had a lower content than Del Cid, Peraleja and Ferragnès (Sk/Sk). Despite the lack of differences between Sk/Sk and Sk/sk sweet cultivars, we tried to classify the cultivars of an un- known genotype into these groups. So, Bonita, CEBAS and Tioga may be Sk/Sk and Achaak, Ferraduel and Pajarera Sk/sk. The rest of the sweet cultivars could not be classified based on the amygdalin content. It has been shown that the heterozygous slightly bitter cultivars, like Garrigues, tend to generate more slightly bitter descendants than other heterozygous sweet cultivars (Dicenta and García, 1993). Therefore, the slightly bitter cultivars may pass to the offspring the capacity to produce the slightly bitter taste, apart from the level of amygdalin. Conclusions We can conclude that there is a high variability in amygda- lin content among the studied cultivars, being this content characteristic for each variety and quite stable year by year. TABlE 3. ANOVA of amygdalin content among varieties and years (1997, 1998), for the sweet and slightly bitter varieties. SV Sum of squares DF Mean square F. ratio Significance F Main effects 9196.734 23 399.858 4761.249 < 0.001 Year 1.930 1 1.930 22.985 < 0.001 Variety 9176.640 22 417.120 4966.793 < 0.001 Year x variety 16.713 17 0.983 11.707 < 0.001 Explained 9213.448 40 230.336 2742.693 < 0.001 Residual 6.887 82 0.084 Total 9220.334 122 75.577 264 Agron. Colomb. 30(2) 2012 The bitter cultivars showed amygdalin contents much higher than the non-bitter ones; and slightly bitter ones had higher contents than sweet ones, but with some exceptions. Finally, the amount of amygdalin is not a useful tool to identify Sk/Sk and Sk/sk cultivars. 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