Received for publication: 6 October, 2014. Accepted for publication: 27 November, 2014. Doi: 10.15446/agron.colomb.v32n3.46031 1 La Selva Research Center, Corporacion Colombiana de Investigacion Agropecuaria (Corpoica). Rionegro (Colombia). cpassaro@gmail.com 2 Westfalia Technological Services. Limpopo (South Africa). Agronomía Colombiana 32(3), 399-406, 2014 Determination of the minimum dry matter index for the optimum harvest of ‘Hass’ avocado fruits in Colombia Determinación del índice mínimo de materia seca para la óptima cosecha del aguacate ‘Hass’ en Colombia Catarina Pedro Carvalho1, María Alejandra Velásquez1, and Zelda Van Rooyen2 ABSTRACT RESUMEN Colombia has become an important producer of ‘Hass’ avocado in the last three years; however, a minimum dry matter content has not been established as a maturity index for harvest. The aim of this study was to determine the correlation between oil percentage and dry matter content in order to establish a minimum harvest index for ‘Hass’ avocado fruits grown in Colombia. Samples were collected for maturity determina- tions over three years of ‘Hass’ avocado fruit cultivation from fifteen different orchards in the department of Antioquia in order to determine the dry matter and oil percentage of fruits throughout the season. A simple linear relationship between oil content and dry matter was thus established. The equations for all of the studied orchards presented a good correlation coefficient, ranging between 0.70 on the Cartucho orchard in the municipality of Retiro and 0.99 on the Gacamayas and Paraiso orchards in the municipality of Entrerrios and Retiro. Using the minimal oil standard of 11.2% as a reference, the orchards that were found to have a high dry matter percentage at harvest were Cartucho in Retiro (26%), followed by Piedras Blancas and Santa Cruz in the municipality of Venecia (25%); while the ‘Hass’ avocado fruit from the Cebadero orchard in Retiro, Coconi in the municipality of La Ceja and Guacamayas in Entrerrios reached this oil standard at 22% dry matter, which could be a commercial advantage. Based on these oil content results, a minimal dry matter index of 23.5% was proposed as a harvest maturity indicator for ‘Hass’ avocado grown in Colombia. This harvesting index will need to be refined over time and with the addition of samples from more regions and climatic data profiles. Colombia se ha convertido en un importante productor de aguacate ‘Hass’ en los últimos tres años. Sin embargo, no se ha establecido aún un contenido mínimo de materia seca para cosecha. El objetivo de este estudio fue determinar la correlación entre el porcentaje de aceite y material seca para definir un índice mínimo de cosecha para el aguacate ‘Hass’ cultivado en Colombia. Se recogieron muestras de frutos de aguacate ‘Hass’ durante tres años en quince fincas diferentes en el departamento de Antioquia para determinar el porcen- taje de materia seca y aceite de la fruta durante la temporada. Se estableció una relación lineal simples entre el contenido de aceite y materia seca para el departamento de Antioquia. Las ecuaciones para todas las fincas estudiadas presentaron un buen coeficiente de correlación, que osciló entre 0,70 en la finca Cartucho del municipio de Retiro y 0,99 en las fincas Gacamayas y Paraíso de los municipios de Entrerrios y Retiro, respectivamente. Utilizando como referencia el 11,2% como el estándar mínimo de aceite para cosecha, las fincas que regis- traron un porcentaje de materia seca más alto para cosecha fueron Cartucho del Retiro (26%), seguida de Piedras Blancas y Santa Cruz para el municipio de Venecia (25%), mientras que las fincas Cebadero del Retiro, Coconi del municipio de La Ceja y Guacamayas de Entrerrios, alcanzaron este mismo porcentaje con 22% de materia seca, lo que puede ser consi- derado una ventaja comercial. A partir de estos resultados de contenido de aceite un índice mínimo de materia seca de 23,5% fue propuesto como indicador de la madurez para cosecha de aguacate ‘Hass’ cultivado en Colombia. Este índice de cosecha deberá ser refinado con el tiempo y con la adición de muestras de más regiones y datos climáticos. Key words: Persea americana Mill., oil percentage, maturity index, quality, region, Antioquia. Palabras clave: Persea americana Mill., porcentaje de aceite, índice de madurez, calidad, región, Antioquia. Introduction Colombia exported several containers of ‘Hass’ avocado (Persea americana Mill.) to Europe in the last few years. The country, during that time, had about 6,300 ha of ‘Hass’ avocado production spread over 9 provinces. The national production amounted to 27,532 t, with an average yield of 4.03 t ha-1 and 19 kg/tree. ‘Hass’ avocado production made up 25.4% of the cultivar spread in the nation (Mejía, 2012). Avocado is a climacteric fruit that does not ripen on the tree, so it must be harvested during the suitable physiological 400 Agron. Colomb. 32(3) 2014 maturity stage to achieve the edible characteristics of taste and firmness (Gil, 2000; Gamble et al., 2010). It is very hard to visually determine the appropriate maturity stage in the ‘Hass’ avocado for harvesting because the fruit does not exhibit any notable external change in appearance (Kas- sim et al., 2013). Thus, it is not uncommon to find ‘Hass’ avocado fruits in the market at the expected maturity stage with an uncharacteristic color and a shriveled peel, especially early in the season (Osuna-García et al., 2010; Pedreschi et al., 2014). During the maturation process, there is an increase in the oil content of the fruit, a moisture reduction and an increase in palatability (Ozdemir and Topuz, 2004; Osuna-García et al., 2010). A popular method for determining oil content in the avocado is the Soxhlet method (Lee, 1981). However, it is slow, expensive and difficult to perform; thus, people prefer to use the indirect method of using the percentage of dry matter of fruit pulp as an indication of maturity. This method is based on the high correlation between a decrease in fruit moisture content and an increase in dry matter or an increase in the oil content of fruit (Lee et al., 1983; Woolf et al., 2004). The use of the percentage of dry matter as a maturity indi- cator for avocado is widely accepted and minimum values have been established as a legal standard for each cultivar in most countries. The minimum requirement of dry matter varies from 19 to 25%, depending on the cultivar (19.0% for Fuerte, 20.8% for Hass and 24.2% for Gwen) and the country (21% for Australia, 21.6-22.8% for USA and 23.0% for Mexico, South America and South Africa for 'Hass' avocado) (Hofman et al., 2002; Orhevba and Jinadu, 2011; Kassim et al., 2013). Fruits harvested with dry matter levels below the recom- mended minimum will ripen irregularly and will not fully develop their quality attributes. Similarly, fruits harvested with a high dry matter undergo rapid ripening and have a reduced shelf life (Wu et al., 2011). Studies by Whiley et al. (1996) indicated that early harvesting of late-maturing ‘Hass’ at 25 to 30% dry matter resulted in high productiv- ity; whereas, harvesting at 35% dry matter reduced yields, also leading to alternate bearing. The oil content in avocados depends on several factors, such as the cultivar (Chen et al., 2009; Dodd et al., 2010; Orhevba and Jinadu, 2011), agro-ecological conditions of growth (Landahl et al., 2009; Kassim et al., 2013; Wedding et al., 2013; Donetti and Terry, 2014) and the fruit development stage (Ozdemir and Topuz, 2004; Osuna-García et al., 2010; Villa-Rodríguez et al., 2011). Since 1925, a minimum standard of 8% oil content in the pulp of avocado fruit was used in the California Avocado Industry in the United States, but since the eighties they began using minimum oil content percentages for each cultivar (e.g. 10.0% for Fuerte and 11.2% for Hass) (Anon, 1925; Lee et al. 1983; Ozdemir and Topuz, 2004; Dodd et al., 2010). Lee et al. (1983) examined the relationship between dry weight, oil and sensory perception. They concluded that regional harvest dates were not appropriate. There was also discussion as to whether the same dry matter level means the same “taste acceptability” in different countries or, even possibly, in different regions within a country. In Colombia, genetic and agro-ecological variability are very high, hindering homogeneous fruit production and management and the prediction of the correct harvest date. Bearing these conditions in mind, it is rather chal- lenging to make Colombian ‘Hass’ fruits competitive and to create synergy with regional countries such as Mexico and Chile. The correlation between oil and dry matter percentage for ‘Hass’ avocado fruit has not been established yet in Colombia. However, in relation to the growing area, the determination of the minimum percentages of oil and/or dry matter should help determine when to harvest. Limits on harvest maturity should be set in order to achieve stan- dardization of fruit quality for an export-based industry. The aim of this study was to determine the correlation between oil percentage and dry matter content to establish a minimum harvest index for ‘Hass’ avocado fruits grown in Colombia. This will be a useful tool for farmers target- ing the export market and aiming to deliver fruits at the optimum commercial maturity and quality, using adequate transport, storage and shelf life. Materials and methods Plant material To determine the relationship between the dry matter and oil percentage of ‘Hass’ avocado in different municipalities of the department of Antioquia, Colombia, we selected di- fferent orchards at different altitudes above sea level (Tab. 1). In each orchard, one tree of the same age and with a normal level of production were selected in a homogeneous 401Carvalho, Velásquez, and Van Rooyen: Determination of the minimum dry matter index for the optimum harvest of ‘Hass’ avocado fruits in Colombia area of the plot. Eight fruits of different maturity stages, determined by the size and bright of the peel, were harves- ted to build a maturation curve. At least two harvests were done for each orchard and the evaluation was carried out between 2011 and 2013 (Tab. 1). After harvest, the fruits were immediately brought to the Postharvest Laboratory of the La Selva Research Center of Corpoica (Corporacion Colombiana de Investigacion Agropecuaria) in Antioquia to determine dry matter. The samples of dry avocado pulp were stored at ambient room conditions inside a desiccator with silica gel until the oil percentage was determined. Dry matter The dry matter (DM) was determined according the Lee method (Lee, 1981). The samples were dried at 60ºC un- til they reached a constant weight. The final and initial weight differences were used to calculate the dry matter percentage. TABlE 1. Orchards selected for the study of maturity index of ‘Hass’ avocado in the department of Antioquia, Colombia. Municipalities Orchard Altitude (m a.s.l.) latitude (N) longitude (W) Orchard age (years) Orchard area (ha) Harvest date Támesis La Maria 1,340 05°41’21.6” 75°42’15.7” 5 to 6 - 23/08/2011 29/06/2012 Venecia Piedras Blancas 1,510 05°55’58.0’’ 75°45’33.4’’ 5 16 16/08/2011 22/06/2011 03/09/2012 Venecia Santa Cruz 1,770 05°55’50.3’’ 75°46’53.1’’ 4 20 05/10/2011 17/08/2011 14/06/2012 Jericó El Encanto 1,900 05°47’48.7” 75°45’45” 3 6 06/10/2011 23/08/2012 Rionegro Yeguas 2,183 06°08’28.1’’ 75°27’28.8’’ 4 a 8 8.17 19/12/2012 27/12/2012 30/01/2013 El Retiro Cartucho 2,229 06°04’58’’ 75°27’22.8’’ 4 a 7 4 17/12/2012 26/12/2012 30/01/2013 El Retiro Guarango 2,244 06°02’57.2’’ 75°29’48’’ 6 9.8 10/12/2012 17/12/2012 14/02/2013 Rionegro La Escondida 2,248 06°05’53’’ 75°44’20” 5 14 29/09/2011 21/06/2012 El Retiro Casaloma 2,267 06°01’42’’ 75°30’16.1’’ 4 0.2 11/12/2012 28/12/2012 30/01/2013 El Retiro Isabela 2,303 06°00’57.4’’ 75°29’28.7’’ 7 1.43 07/12/2012 27/12/2012 14/02/2013 El Retiro Cebadero 2,364 06°01’46.7’’ 75°27’51.1’’ 3 a 5 40 07/12/2012 18/12/2012 14/02/2013 La Ceja Coconi 2,381 06°00’41.9’’ 75°26’10.2’’ 6 0.42 14/12/2012 26/12/2012 30/01/2013 La Ceja Entreaguas 2,383 05°57’18.3’’ 75°25’10.3’’ 5 9.41 13/12/2012 26/12/2012 30/01/2013 El Retiro Paraiso 2,383 06°05’10’’ 75°27’23’’ 5 - 17/12/2012 26/12/2012 30/01/2013 Entrerrios Guacamayas 2,420 06°33’39.4’’ 75°32’28.6’’ 5 5.39 28/07/2011 07/06/2012 402 Agron. Colomb. 32(3) 2014 Oil percentage The oil percentage of the dry samples was determined by the Horwitz (1980) and Lee methods (Lee, 1981). A 10 g sample of dry avocado pulp was used to extract the lipids by Soxhlet for 6-8 h using petroleum ether as a solvent. The oil percentage of the avocado pulp was calculated with the following equation and expressed as % (w/w). Oil content (% w/w) = dry matter (%) x oil weight (g) (1) dry pulp weight (g) The dry matter and oil content values were further used to estimate the correlation coefficient and a linear regression model for each orchard. Statistical analysis The results were analyzed by the Statgraphics® Centurion XVI v. 15.2 (StatPoint®, Herndon, VA). A simple linear regression model was applied for determining the rela- tionship between the oil and dry matter percentages. Then an analysis of variance (ANOVA) test was performed to determine the representativeness of the model with a LSD test at 95% level of confidence. Results and discussion The results showed a positive correlation between the dry matter and oil percentages of the fruit pulp for all the studied orchards during the three years of evaluation (Figs. 1 and 2). A close relationship between oil content and dry matter content was thus confirmed (Tab. 2). An increased lipid concentration in fruit as a result of a reduced percentage of water has been reported by many authors (Chen et al., 2009; Gamble et al. 2010; Villa-Rodríguez et al., 2011). Fruit samples from all of the analyzed orchards presented a good correlation coefficient, with the lowest at 0.84 on the Cartucho orchard in Retiro and the highest at 0.99 on the Gacamayas and Paraiso orchards in Entrerrios and Retiro. These correlation values are similar to those reported by other authors for avocado fruits (Olaeta et al., 1986; Chen et al., 2009). The degree of correlation obtained between the oil con- tent and dry matter of avocado fruit pulp was important because the direct estimation of oil percentage of avocado fruits is difficult, slow and expensive. With the current results, we can indirectly and reliably estimate the oil content of fruits using dry matter values in a linear re- gression equation, which is practical and easy (Lee, 1981). The regression equation for each orchard is presented in Tab. 2. With this equation, a farmer can estimate the oil content of a fruit sample based on the determined dry matter percentage. The dry matter percentage is often used as a maturity index in most avocado producing areas in the world. In the val- leys of the central coast of Peru, ‘Hass’ avocado is usually harvested with a minimum oil content of 8-9% and with a 20-21% dry matter content (Franciosi, 2003). The ‘Hass’ Avocado Committee of Chile authorizes the commercial harvest of ‘Hass’ fruit with a minimal dry matter content of 23% (Waissbluth and Valenzuela, 2007). FIgURE 1. Correlation between oil content and dry matter for ‘Hass’ avo- cado grown in different orchards of department of Antioquia, Colombia between 2011 and 2012. 11.2% O il co nt en t ( % w /w ) Dry matter (%) 0 5 10 15 20 25 30 35 40 0 5 10 15 20 25 La Escondida Guacamayas El Encanto La María Piedras Blancas Santa Cruz 23%21.5% 11.2% O il co nt en t ( % w /w ) Dry matter (%) 0 5 10 15 20 25 30 35 40 0 5 10 15 20 25 Cartucho Casaloma Cebadero Cebadero Entreaguas Guarango Isabela Paraiso Yeguas 23%21.5% FIgURE 2. Correlation between oil content and dry matter for ‘Hass’ avocado grown in different orchards of the department of Antioquia, Colombia, between 2012 and 2013. 403Carvalho, Velásquez, and Van Rooyen: Determination of the minimum dry matter index for the optimum harvest of ‘Hass’ avocado fruits in Colombia Law number 422 of California, established in 1925, speci- fied 8% as the minimal oil fruit content before harvesting could commence (Anon, 1925). Nevertheless, the oil con- tent of fruit that was considered acceptable for consumption differed among cultivars and the 8% requirement was too low to serve as a good maturity standard for many cultivars according (Lee et al., 1983; Chen et al., 2009; Dodd et al., 2010). In addition, while the date of acceptable taste of fruit grown at the same location was not significantly different from year to year, it varied significantly among and within the widespread avocado production areas. Morton (1987), based on experiences in Mexico, stated that fruit reaches a good taste when the fruit has a minimum oil content of 8% and dry matter of 21%. Olaeta et al. (1986) defined the minimal oil content for ‘Hass’ avocado in Chile as 10%. After the eighties, California (USA) started using higher minimal oil percentages and discerning between cultivars; for example: 10.0% for Fuerte and 11.2% for Hass (Lee et al., 1983). It is also important to note that fruit quality starts to dete- riorate when dry matter is too high. Picking overly-mature fruits results in poor f lavor and increased fruit diseases and f lesh disorders, as well as reduced yields of the next crop (Whiley et al., 1996; Osuna-García et al., 2010; Wu et al., 2011). Fruit should not be left on trees beyond the normal picking period. For this reason, a maximum cut-off for harvesting ‘Hass’ avocado fruits should be defined. Thorp et al. (1997), studying the postharvest quality of New Zea- land‐grown ‘Hass’ avocado, observed that fruit harvested with a dry matter above 26% had severe rot. Prior to the current research study, Colombian growers and fruit handlers had no standard index defined for the com- mercial harvest of ‘Hass’ avocado. In this study, we used the standards of other countries as a reference; for example, dry matter between 21.5 and 23% and an oil content of 11.2% to see when the different orchards reach the physiological mature stage for harvest (Figs. 1 and 2). The standard oil percentage was considered to be a better harvest indicator for avocado because it relates well with fruit f lavor. As can be seen in Fig. 1, none of the studied orchards with a dry matter of 21.5% reached an oil content of 11.2%, nor did the studied orchards in Fig. 2. From both figures, we can see that all of the studied orchards reached the same oil content, but with different accumulation rates (slope of the straight), reaching the physiological mature index at different times. When we compared the different orchards for the physi- ological standard of oil content (11.2%), the percentage of dry matter ranged from 22 to 26% (Tab. 2). It is interesting to note that ‘Hass’ avocado fruit from the Cebadero orchard in Retiro, Coconi in La Ceja and Guacamayas in Entrer- rios reached this oil standard earlier (for 22% dry matter) when compared to the other orchards, which could be a commercial advantage. The orchards with a high dry mat- ter percentage for harvest in this study were Cartucho in Retiro (26%), followed by Piedras Blancas and Santa Cruz in Venecia (25%). More evaluations over time and for more orchards are needed for a better understanding of the effect of altitude TABlE 2. Regression equations of oil percentage and dry matter for ‘Hass’ avocado in different municipalities of department of Antioquia, Colombia. Municipalities Orchard Altitude (m a.s.l.) Equation Correlation coefficient P<0.05* Dry matter (%) for 11.2% of oil Támesis La Maria 1,340 Oil (%) = -10.90 + 0.95*DM (%) 0.98 0.0000 23 Venecia Piedras Blancas 1,510 Oil (%) = -8.02 + 0.76*DM (%) 0.92 0.0000 25 Venecia Santa Cruz 1,770 Oil (%) = -12.66 + 0.97*DM (%) 0.94 0.0000 25 Jericó El Encanto 1,900 Oil (%) = -12.19 + 1.03*DM (%) 0.97 0.0000 23 Rionegro Yeguas 2,183 Oil (%) = -11.82 + 0.99*DM (%) 0.97 0.0000 23 El Retiro Cartucho 2,229 Oil (%) = -8.63 + 0.76*DM (%) 0.84 0.0006 26 El Retiro Guarango 2,244 Oil (%) = -14.23 + 1.08*DM (%) 0.95 0.0000 24 Rionegro La Escondida 2,248 Oil (%) = -11.65 + 0.99*DM (%) 0.99 0.0000 23 El Retiro Casaloma 2,267 Oil (%) = -8.89 + 0.86*DM (%) 0.89 0.0001 23 El Retiro Isabela 2,303 Oil (%) = -9.76 + 0.89*DM (%) 0.96 0.0000 23 El Retiro Cebadero 2,364 Oil (%) = -13.15 + 1.09*DM (%) 0.96 0.0000 22 La Ceja Coconi 2,381 Oil (%) = -3.18 + 0.65*DM (%) 0.98 0.0000 22 La Ceja Entreaguas 2,383 Oil (%) = -7.04 + 0.80*DM (%) 0.90 0.0000 23 El Retiro Paraiso 2,383 Oil (%) = -14.03 + 1.06*DM (%) 0.99 0.0000 24 Entrerrios Guacamayas 2,420 Oil (%) = -15.08 + 1.19*DM (%) 0.99 0.0000 22 * For a P-value of ANOVA inferior to 0.05, there was a statistical relationship between oil and dry matter (DM) with a confidence level of 95.0%. 404 Agron. Colomb. 32(3) 2014 and climate on physiological maturity and variables such as PAR radiation, solar radiation and accumulated precipita- tion must be considered in the evaluation. Different authors (Kaiser et al., 1992; Kruger, 1999; Landahl et al., 2009; Donetti and Terry, 2014) reported that the oil and moisture contents of ‘Hass’ and ‘Fuerte’ avocado, at the same date, vary year by year; probably because of the climatic conditions for a specific year and the rainfall and temperature variation of the seasons. They observed that the accumulation of dry matter percentage can be higher in seasons with higher rainfall and longer exposure to solar radiation. Woolf et al. (1999) confirmed that sun-exposed ‘Hass’ avocado fruits had higher contents of dry matter, potassium, calcium and magnesium. These fruits took longer to mature and the side exposed to the sunlight had higher firmness as compared to the unexposed side. Waissblut h and Va lenzuela (2007) inferred t hat, in Chile, fruit maturity is more related to altitude than to the north/south orientation. According to Ferreyra and Defilippi (2012), the orchards on the coast of Chile take 55 d longer to reach 23% dry matter than the ones in the central valley zone due to climatic conditions and also probably due to agronomic practices (nutrition, prun- ing, orchard age, orchard density, and others). The same authors established the maximum limit of dry matter for a good shelf life and fruit quality at 27.5% (> 27.5% high risk and < 27.5% low risk). The linear model with all the data collected between 2011 and 2013 from different orchards of Antioquia is shown in Fig. 3. The model exhibited a statistical significance at 95% for the relationship between oil and dry matter and a good fit, explaining 93.4% of the oil content variability. The coefficient of 0.95 indicated a strong relationship between the two variables. The equation in Fig. 3 can be used by any grower in Antioquia, although the model needs to be refined with more data over time (harvest seasons and years) and with fruit from more orchards to be more ac- curate. However, according to this model, the oil content standard of 11.2% corresponded to a dry matter of 23.5%. This could be considered the minimal index at which one can harvest ‘Hass’ avocado in Antioquia and guarantee good taste and fruit quality, despite the variation that might exist between orchards as seen in Tab. 2. It is also important to define a maximum harvest index of dry matter percentage related to shelf life and fruit quality during transport and storage. Colombia must define clear rules for establishing the adequate grade and harvest date according to the cultivar and producing region. Until now, there has been no scientific evidence and, consequently, harvesting has been done based on past (empirical) agri- cultural experience: namely fruit color and size. A protocol for sampling the orchards with the minimun dry matter index for ‘Hass’ avocado at harvest must now be defined by governmental entities. In addition to the issues involved in simply measuring dry matter, an important area which requires greater understanding is the relationship between oil content (dry matter) and various f lavor attributes of avocados and not just an “overall consumer acceptability”. According to Obenland et al. (2012), neither dry matter nor oil per- centage are adequate in of themselves to fully explain the differences in the eating quality of avocados so additional means of assessing eating quality would be desirable. The development of sensory descriptors and their relationship with aroma volatiles for ‘Hass’ avocado in Colombia could be useful for better linking maturity changes with f lavor. This might help in examining regional effects in terms of oil content levels and f lavor (Gamble et al., 2010; Paull and Duarte, 2011; Yahia and Woolf, 2011). A correlation superior to 0.93% between fruit taste and oil percentage was observed by Olaeta et al. (1986) when studying different avocado cultivars (Fuerte, Bacon, Edranol, Hass, Butano and Negra de La Cruz). Conclusions The oil content of the fruits showed a positive highly sig- nificant correlation (superior to 0.80) with the percentage FIgURE 3. Linear model of relation between oil content and dry matter for ‘Hass’ avocado in department of Antioquia, Colombia. 11.2% O il co nt en t ( % w /w ) Dry matter (%) 0 5 10 15 20 25 30 35 40 0 5 10 15 20 25 La Escondida Guacamayas El Encanto La María Piedras Blancas Santa Cruz Cartucho Casaloma Cebadero Cebadero Entreaguas Guarango Isabela Paraiso Yeguas 23.5% 405Carvalho, Velásquez, and Van Rooyen: Determination of the minimum dry matter index for the optimum harvest of ‘Hass’ avocado fruits in Colombia of dry matter for all of the studied orchards. An equation to estimate oil percentage in relation to dry matter percent- age was defined with a good fit for each studied orchard. A simple linear model was defined for Antioquia to determine oil content in relation to dry matter percentage with a very high correlation coefficient. This is a very useful tool for ‘Hass’ avocado producers because the determination of oil content is very expensive. In this way, they can predict that the fruits will meet the required optimum postharvest quality. Using the minimal oil standard of 11.2% as a refer- ence, the minimal dry matter index for harvesting ‘Hass’ avocado in Colombia was defined as 23.5%. Nevertheless, more evaluations over time (harvest seasons and years) and with more regions and climatic data are needed to develop a more accurate model. As Colombia is a country with a very high climate variability over short distances, a model of oil and dry matter percentage for each producing region should be developed. The minimal oil content needed to reach physiological maturity and a good f lavor for ‘Hass’ avocado must also be defined for Colombian ‘Hass’ fruits grown in different regions. Acknowledgments Part of this work was financially supported by the Secreta- ria de Agricultura y Desarrollo Rural of Antioquia through project No. 2010.SS.1800.09 and by the Ministerio de Agri- cultura y Desarrollo Rural of Colombia through project No. 2012-211. Thanks are given to professor Juan Carlos Perez Naranjo of the Universidad Nacional de Colombia for proofreading this article. literature cited Anon. 1925. Standardization committee report California Avocado Association Annual Report. California Avocado Association, Pasadena, CA. pp. 46-47. Chen, N.J., M.M. Wall, R.E. Paull, and P.A. Follett. 2009. Variation in ‘Sharwil’ avocado maturity during the harvest season and resistance to fruit f ly infestation. HortScience 44, 1655-1661 Dodd, M., P. Cronje, M. Taylor, M. Huysamer, F. Kruger, E. Lotz, and K. 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