PERSISTENT ORGANIC POLLUTANTS IN THE LIVERS OF MOOSE HARVESTED IN THE SOUTHERN NORTHWEST TERRITORIES, CANADA Nicholas C. Larter1, Derek Muir2, Xiaowa Wang2, Danny G. Allaire1, Allicia Kelly3, and Karl Cox3 1Department of Environment & Natural Resources, Government of the Northwest Territories, PO Box 240, Fort Simpson, Northwest Territories, Canada X0E 0N0; 2Aquatic Contaminants Research Division, Environment and Climate Change Canada, Burlington, Ontario, Canada L7S 1A1; 3Department of Environment & Natural Resources, Government of the Northwest Territories, PO Box 900, Fort Smith, Northwest Territories, Canada X0E 0P0. ALCES VOL. 53: e1–e33 (2017) ANALYTICAL METHODS PCBs, OCOs, and BFRs Samples were thawed and thoroughly homogenized in a small stainless steel blend- er. Subsamples (3.5 to 5 g ww) were Soxhlet- extracted with dichloromethane; all were spiked with 1,3,5-tribromobenzene (TrBB) prior to extraction. Recovery of brominated compounds was monitored using BDE-71, d16-gHBCD, and C13-BDE-209. 13C12-PCB- 133 was added as a recovery standard for GPC performance for samples analyzed by ALS Global. A laboratory blank consisting of all reagents and 2 NIST reference materials (cod liver SRM 1588b and fish muscle SRM 1946) were also analyzed with the 14 sam- ples. The extracts were then rotary evapo- rated under vacuum, exchanged into DCM: hexane (1:1) and applied to a gel permeation chromatography (GPC) column (60 g Bio- beads SX3) to remove lipids and other biogenic materials. The GPC eluate was reduced to 1 mL under vacuum. Percent lipid was determined gravimetrically on a sub- sample of the extract or by evaporating the first GPC fraction. Extracts were cleaned up on a silica gel column. NLET utilized activated silica gel (8 g, 1.1 cm i.d. column), and eluted with hexane followed by n-hexane/DCM (1:1) to separate PCBs from most of the PBDEs and OCPs. BDE-209 was quantitatively eluted in the silica Fraction 1. The ALS Global split the GPC elution into separate OCP/OCO fractions that were chromatographed on a 2% deactivated silica gel column and then reduced to 0.05 mL for analysis. The PCB fraction was cleaned up on an acid-silica gel column (45% w/w H2SO4 on Silica Gel topped with neutral Silica Gel) then reduced to 0.04 mL for anlaysis. GC-ECD analysis was conducted on 7 Dehcho samples using a GC-ECD (Agilent 6890 gas chromatograph with a 63Ni-electron capture detector [ECD]) using a 30 m x 0.25 mm (i.d.) DB-5 column (internal film thick- ness 0.25 mm; J&W Scientific, Folsom, California, USA) with H2 carrier gas (con- stant flow rate 0.91 mL min-1). Ultra-pure N2 was used as the makeup gas for the ECD (detector temperature: 325 C). The GC-ECD quantification of OCs in each sample was performed using a 4-point external standard calibration curve. Calibration standards were quantified after every 10 samples. Toxaphene-related compounds, including 22 polychlorinated bornane congeners as well as α- and β-endosulfan and endosulfan sulfate, were quantified by GC-electron cap- ture-negative ion mode (ECNI) mass spec- trometry using an Agilent 6890 GC-5975 1 MS system as described by Hoekstra et al. (2002). Toxaphene and homologues were quantified using a “Hercules” technical standard as described by Glassmeyer et al. (1999). Individual polychlorinated bornane congeners were quantified using a series of ex‐ ternal calibration standards (Dr. Ehrenstofer, Augsburg, Germany). Alpha- and beta- endosulfan, and endosulfan sulfate were quantified by external standards using the characteristic fragment ions m/z 406 and m/z 273. Analyses of all PBDEs and other bromi- nated flame retardants (BFRs) was carried out by GC-ECNIMS on an Agilent 6890- 5975 MS using an HP5-MS capillary column (30 m x 0.25 mm x 0.25 um film thickness). Helium was the carrier gas, and separation was performed at a constant flow of 1.2 mL/min (Muir et al. 2006). The mass spectrometer was operated in the NCI mode, methane was the buffer gas, and temperature was 106, 150, and 300 °C for the quadrupole, the ion source, and the interface, respectively. The analytes were monitored at m/z 79/81 using an external standard calibration, except for C13-BDE-209 which was monitored at m/z 493/495 and native BDE-209 at m/z 487/ 485. Any β- and γ-HBCDD residues in the samples were most likely thermally isomer- ized to α-HBCDD in the GC injection port, thus, results represent total HBCDD (Muir et al. 2006). PFASs An internal standard mixture of 13C-PFASs was added to every sample and extracted by shaking twice with acetonitrile. The extract was evaporated under nitrogen to dryness and reconstituted with 1 mL of methanol. The extract was cleaned with a graphite carbon solid phase cartridge (Supelco). Cleaned up extracts were analyzed for PFCAs as well as PFSAs. The analyses were performed by liquid chromatography with negative electrospray tandem mass spectrometry (LC-MS/MS). Analytes were detected using an API 4000 Q Trap (Applied Biosystems, Carlsbad, California, USA) after chromatographic separation with an Agilent 1100 LC. Chromatography was performed using an ACE C18 column (50 mm x 2.1 mm, 3 µm particle size; Aberdeen, United Kingdom), preceded by a C18 guard column (4.0 x 2.0 mm, Phenomenex) and the column oven was set to 30 °C. Samples were quanti- fied with a 6 point calibration curve and isotopic dilution method. QUALITY ASSURANCE Recoveries of internal standards ranged from 79% for δ-HCH to 120% for PCB-204 (Table S4) in Dehcho samples analysed by GC-ECD, and from 59% for endrin ketone to 138% for 1245-TTBB in samples analysed by GC-HRMS/LRMS. A recovery spike demonstrated good recoveries of 32 OCP/ OCOs (55-101%) and 18 PBDE/BFRs (71– 133%) (Table S5). Slight losses of more vola- tile compounds (e.g., hexachlorobutadiene) and recovery enhancement due to contri‐ bution from laboratory reagent blanks (BDE 47) explain the recovery variation. No correc- tions for recovery were made based on this information. Analysis of the reference mate- rials (NIST SRMs 1588b and 1946) showed good agreement with all analytes quantified to within ±25% of certified values of OCP/OCOs (17 compounds) and PCBs (29 congeners). 2 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 1. L is t of m oo se sa m pl es , co lle ct io n ye ar , ag e, se x, bi ol og ic al ch ar ac te ri st ic s, ha rv es t da te an d lo ca tio n. R eg io n ID A ge S ex C on di tio n E /G /F 1 H un te r es t. ag e D at e H ar ve st ed L at itu de o N L on gi tu de o W S ou th S la ve S S R -M O -1 1- 4 < 1 M F C al f 20 -F eb -1 0 60 .2 1 11 2. 13 S S R -M O -1 1- 7 1 M E A du lt 10 -J an -1 0 60 .6 4 11 2. 27 S S R -M O -1 1- 8 4 M n/ a A du lt 4- O ct -1 0 60 .7 6 11 2. 19 S S R -M O -1 1- 9 4 M G A du lt 13 -D ec -1 0 60 .7 9 11 6. 22 S S R -M O -1 1- 10 < 1 M G Y ea rl in g 30 -S ep -1 0 60 .8 5 11 4. 49 S S R -M O -1 1- 12 < 1 F G ca lf 8- F eb -1 0 60 .7 8 11 2. 78 S S R -M O -1 1- 13 9 F n/ a A du lt 8- F eb -1 0 60 .7 8 11 2. 78 D eh ch o G E N -1 4 3 M G A du lt 21 -F eb -0 6 61 .5 5 12 1. 18 G E N -1 5 6 F G A du lt 02 -M ar -0 6 61 .6 4 12 1. 06 G E N -1 6 12 F G A du lt 06 -M ar -0 6 61 .6 0 12 1. 14 G E N -1 7 < 1 F G C al f 06 -M ar -0 6 61 .6 0 12 1. 14 G E N -1 8 < 1 M G C al f 24 -M ar -0 6 61 .5 0 12 0. 62 G E N -1 9 2 M E A du lt 18 -M ar -0 6 61 .4 8 12 0. 62 JM R -4 5 F E A du lt 24 -M ar -0 6 61 .4 9 12 0. 64 1 H un te r co nd iti on ev al ua tio n. E = ex ce lle nt , G = go od , F = fa ir . n/ a = no t av ai la bl e. ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 3 Ta bl e S2 .L is to f in di vi du al or ga no ha lo ge n an al yt es al on g w ith th ei r M D L s (n g/ g w w ) us in g ei th er G C w ith hi gh or lo w re so lu tio n M S, G C -N C IM S ,o r G C -E C D . M D L 3 M D L C la ss 1 C om m on na m e C he m ic al or ot he r na m e In st ru m en ta l an al ys is G C -M S G C -E C D O C O H ex ac hl or ob ut ad ie ne G C -H R M S , G C -E C D < 0. 00 5 0. 03 2 O C O 1, 2, 4, 5- T et ra ch lo ro be nz en e 1, 2, 4, 5- te tr ac hl or ob en ze ne G C -H R M S , G C -E C D 0. 09 9 0. 00 7 O C O 1, 2, 3, 4- T et ra ch lo ro be nz en e 1, 2, 3, 4- te tr ac hl or ob en ze ne G C -H R M S , G C -E C D 0. 10 8 0. 01 5 O C P P en ta ch lo ro be nz en e pe nt ac hl or ob en ze ne G C -H R M S , G C -E C D 0. 07 7 < 0. 00 2 O C O 34 5- tr ic hl or ov er at ro le G C -H R M S , G C -E C D na 0. 01 2 O C P P en ta ch lo ro an is ol e G C -H R M S , G C -E C D < 0. 00 8 0. 02 4 O C O H ex ac hl or ob en ze ne G C -H R M S , G C -E C D < 0. 02 6 < 0. 00 2 O C O 3, 4, 5, 6- T et ra ch lo ro ve ra tr ol e G C -H R M S , G C -E C D < 0. 01 6 < 0. 01 1 O C P α- H C H α- he xa ch lo ro cy cl oh ex an e G C -H R M S , G C -E C D < 0. 05 9 0. 01 5 O C P β- H C H β- he xa ch lo ro cy cl oh ex an e G C -H R M S , G C -E C D < 0. 1 0. 01 3 O C P γ- H C H lin da ne G C -H R M S , G C -E C D < 0. 07 5 < 0. 00 2 O C P H ep ta ch lo r G C -H R M S , G C -E C D < 0. 01 8 0. 08 O C P P en ta ch lo ro ni tr ob en ze ne G C -H R M S , G C -E C D < 0. 07 5 na O C P A ld ri n G C -H R M S , G C -E C D < 0. 01 1 0. 02 6 O C P D ac th al G C -H R M S , G C -E C D < 0. 05 6 na O C P O ct ac hl or os ty re ne G C -H R M S , G C -E C D < 0. 02 5 0. 01 6 O C P H ep ta ch lo rE po xi de G C -H R M S , G C -E C D < 0. 06 9 0. 04 7 O C P O xy ch lo rd an e G C -H R M S , G C -E C D < 0. 01 9 < 0. 00 2 O C P tr an s- ch lo rd an e G C -H R M S , G C -E C D < 0. 03 7 0. 00 5 O C P ci s- ch lo rd an e G C -H R M S , G C -E C D < 0. 03 2 0. 01 2 O C P tr an s- no na ch lo r G C -H R M S , G C -E C D < 0. 03 5 0. 06 2 O C P D ie ld ri n G C -H R M S , G C -E C D < 0. 01 8 0. 08 8 O C P ci s- no na ch lo r G C -H R M S , G C -E C D < 0. 03 9 0. 02 4 O C P E nd ri n G C -H R M S , G C -E C D < 0. 38 7 0. 02 3 T ab le S 2 co nt in ue d . . . . 4 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 2 co nt in ue d M D L 3 M D L C la ss 1 C om m on na m e C he m ic al or ot he r na m e In st ru m en ta l an al ys is G C -M S G C -E C D O C P 24 '-D D E G C -H R M S , G C -E C D < 0. 01 0. 00 1 O C P 44 '-D D E G C -H R M S , G C -E C D < 0. 01 0. 02 1 O C P 24 '-D D D G C -H R M S , G C -E C D < 0. 01 0. 01 4 O C P 44 '-D D D G C -H R M S , G C -E C D < 0. 01 0. 02 O C P 24 '-D D T G C -H R M S , G C -E C D < 0. 01 0. 02 O C P 44 '-D D T G C -H R M S , G C -E C D < 0. 01 0. 02 O C P M et ho xy ch lo r G C -H R M S , G C -E C D < 0. 01 0. 01 9 O C P M ir ex G C -H R M S , G C -E C D 0. 06 8 0. 01 P C B s P C B -1 m on oc hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 na P C B s P C B -3 m on oc hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 na P C B s P C B 4/ 10 di ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 16 6 P C B s P C B 7/ 9 di ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 15 8 P C B s P C B 6 di ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 04 2 P C B s P C B 8/ 5 di ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 07 8 P C B s P C B 12 /1 3 di ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 1. 11 P C B s P C B 15 di ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 03 6 P C B s P C B 19 tr ic hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 < 0. 00 2 P C B s P C B 18 tr ic hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 21 1 P C B s P C B 17 tr ic hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 na P C B s P C B 27 /2 4 tr ic hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 03 6 P C B s P C B 16 /3 2 tr ic hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 11 9 P C B s P C B 26 tr ic hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 11 2 P C B s P C B 25 tr ic hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 01 9 T ab le S 2 co nt in ue d . . . . ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 5 Ta bl e S 2 co nt in ue d M D L 3 M D L C la ss 1 C om m on na m e C he m ic al or ot he r na m e In st ru m en ta l an al ys is G C -M S G C -E C D P C B s P C B 31 tr ic hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 44 5 P C B s P C B 28 tr ic hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 05 P C B s P C B 20 /3 3/ 21 tr ic hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 15 5 P C B s P C B 22 tr ic hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 09 7 P C B s P C B 37 tr ic hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 P C B s P C B 53 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 11 7 P C B s P C B 45 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 13 6 P C B s P C B 46 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 05 8 P C B s P C B 73 /5 2 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 91 3 P C B s P C B 43 /4 9 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 44 6 P C B s P C B 48 /4 7/ 75 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 5. 15 2 P C B s P C B 44 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 55 4 P C B s P C B 59 /4 2 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 21 P C B s P C B 71 /4 1/ 68 /6 4 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 41 7 P C B s P C B 10 0 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 05 2 P C B s P C B 63 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 02 4 P C B s P C B 74 /6 1 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 24 1 P C B s P C B 70 /7 6 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 66 3 P C B s P C B 80 /6 6 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 37 5 P C B s P C B 56 /6 0 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 22 9 P C B s P C B 81 te tr ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 41 3 P C B s P C B 95 /9 3 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 64 1 P C B s P C B 91 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 14 6 T ab le S 2 co nt in ue d . . . . 6 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 2 co nt in ue d M D L 3 M D L C la ss 1 C om m on na m e C he m ic al or ot he r na m e In st ru m en ta l an al ys is G C -M S G C -E C D P C B s P C B 92 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 15 6 P C B s P C B 84 /9 0/ 10 1/ 89 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 26 P C B s P C B 89 -1 01 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 69 2 P C B s P C B 99 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 31 9 P C B s P C B 11 9 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 02 2 P C B s P C B 83 /1 08 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 08 9 P C B s P C B 97 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 17 2 P C B s P C B 86 /1 11 /1 25 /1 17 /8 7/ 11 6/ 11 5 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 na P C B s P C B 12 0/ 85 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D 0. 05 5 0. 09 2 P C B s P C B 11 0 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 46 1 P C B s P C B 13 6 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 11 4 P C B s P C B 82 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 04 9 P C B s P C B 10 7/ 10 9 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 05 9 P C B s P C B 12 3 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 40 6 P C B s P C B 11 8/ 10 6 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 29 8 P C B s P C B 11 4 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 02 4 P C B s P C B 10 5/ 12 7 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 14 1 P C B s P C B 12 6 pe nt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 na P C B s P C B 15 1 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 17 3 P C B s P C B 13 5/ 14 4 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 65 1 P C B s P C B 13 9/ 14 9 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 na P C B s P C B 13 1/ 16 5/ 14 2 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 02 1 P C B s P C B 14 6 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 05 6 T ab le S 2 co nt in ue d . . . . ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 7 Ta bl e S 2 co nt in ue d M D L 3 M D L C la ss 1 C om m on na m e C he m ic al or ot he r na m e In st ru m en ta l an al ys is G C -M S G C -E C D P C B s P C B 15 3 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 29 3 P C B s P C B 13 2/ 16 8 he pt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 13 5 P C B s P C B 14 1 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 06 8 P C B s P C B 13 7 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 3. 97 1 P C B s P C B 16 3/ 16 4/ 13 8 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 28 3 P C B s P C B 15 8/ 16 0 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 04 4 P C B s P C B 12 9 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 01 8 P C B s P C B 15 9 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 na P C B s P C B 12 8 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 02 8 P C B s P C B 16 7 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 01 P C B s P C B 15 6 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 01 3 P C B s P C B 15 7 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 < 0. 00 1 P C B s P C B 16 9 he xa ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 na P C B s P C B 18 2/ 18 7 he pt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 10 9 P C B s P C B 18 3 he pt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 04 6 P C B s P C B 17 4/ 18 1 he pt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 07 2 P C B s P C B 17 7 he pt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 14 3 P C B s P C B 17 1 he pt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 04 2 P C B s P C B 17 2/ 19 2 he pt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 01 8 P C B s P C B 19 7 oc ta ch lo or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 00 7 P C B s P C B 18 0 he pt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 08 7 P C B s P C B 19 3 he pt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 13 2 P C B s P C B 19 1 he pt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 < 0. 00 1 T ab le S 2 co nt in ue d . . . . 8 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 2 co nt in ue d M D L 3 M D L C la ss 1 C om m on na m e C he m ic al or ot he r na m e In st ru m en ta l an al ys is G C -M S G C -E C D P C B s P C B 17 0/ 19 0 he pt ac hl or ob ip he ny l G C -L R M S , G C -E C D < 0. 00 2 0. 03 P C B s P C B -2 02 oc ta ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 na P C B s P C B 19 9 oc ta ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 00 8 P C B s P C B 19 6/ 20 3 oc ta ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 02 2 P C B s P C B 19 5 oc ta ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 01 5 P C B s P C B 19 4 oc ta ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 00 5 P C B s P C B 20 5 oc ta ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 < 0. 00 1 P C B s P C B 20 8 no na ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 00 2 P C B s P C B 20 7 no na ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 < 0. 00 1 P C B s P C B 20 6 no na ch lo ro bi ph en yl G C -L R M S , G C -E C D < 0. 00 2 0. 01 3 P C B s P C B 20 9 de ca ch lo ro bi ph en yl G C -L R M S , G C -E C D 0. 08 0. 00 4 S um op - an d pp '-D D T re la te d ΣD D T S um ox yc hl or da ne . C & t- ch lo rd an e, c & t- no na ch lo r, he pt ac hl or , he pt ac hl or ep ox id e ΣC H L S um α, β, γ- H C H ΣH C H S um te tr a, P eC B z, H C B ΣC B z S um α- , β + su lf at e Σe nd os ul fa n S um P C B s ΣP C B S um m on o- di C B s Σm on o- di S um tr i C B s Σt ri S um te tr a cB s Σt et ra S um pe nt a C B s Σp en ta S um he xa C B s Σh ex a T ab le S 2 co nt in ue d . . . . ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 9 Ta bl e S 2 co nt in ue d M D L 3 M D L C la ss 1 C om m on na m e C he m ic al or ot he r na m e In st ru m en ta l an al ys is G C -M S G C -E C D S um he pt a C B s Σh ep ta S um oc ta C B s Σo ct a S um no na -d ec a C B s Σn on a- de ca E nd os ul fa n α- E nd os ul fa n < 0. 00 2 E nd os ul fa n β- E nd os ul fa n < 0. 00 2 E nd os ul fa n E nd os ul fa n su lf at e < 0. 00 2 P er fl uo ro su lf on at es P F B S P er fl uo ro bu ta ne su lf on at e L C -M S /M S < 0. 00 1 P er fl uo ro su lf on at es P F H xS P er fl uo ro he xa ne su lf on at e L C -M S /M S < 0. 00 1 P er fl uo ro su lf on at es P F H pS P er fl uo ro he pt an e su lf on at e L C -M S /M S < 0. 00 1 P er fl uo ro su lf on at es P F O S P er fl uo ro oc ta ne su lf on at e L C -M S /M S 0. 29 9 P er fl uo ro su lf on at es P F D S P er fl uo ro de ca ne su lf on at e L C -M S /M S < 0. 00 1 P er fl uo ro su lf on at es P F O S A P er fl uo ro oc ta ne su lf on am id e L C -M S /M S < 0. 00 1 P er fl uo ro su lf on at es ΣP F S A s S um pe rf lu or os ul fo na te s L C -M S /M S P er fl uo ro ca rb ox yl at es P F H xA P er fl uo ro he xa no at e L C -M S /M S 0. 17 9 P er fl uo ro ca rb ox yl at es P F H pA P er fl uo ro he pt an oa te L C -M S /M S 0. 03 6 P er fl uo ro ca rb ox yl at es P F O A P er fl uo ro oc ta no at e L C -M S /M S 0. 21 3 P er fl uo ro ca rb ox yl at es P F N A P er fl uo ro no na no at e L C -M S /M S 0. 04 6 P er fl uo ro ca rb ox yl at es P F D A P er fl uo ro de ca no at e L C -M S /M S 0. 25 6 P er fl uo ro ca rb ox yl at es P F U nA P er fl uo ro un de ca no at e L C -M S /M S 0. 08 5 P er fl uo ro ca rb ox yl at es P F D oA P er fl uo ro un do de ca no at e L C -M S /M S 0. 15 9 P er fl uo ro ca rb ox yl at es P F TA P er fl uo ro tr id ec an oa te L C -M S /M S 0. 08 9 P er fl uo ro ca rb ox yl at es P F T rA P er fl uo ro te tr ad ec an oa te L C -M S /M S 0. 13 1 P er fl uo ro ca rb ox yl at es ΣP F C A s S um pe rf lu or oc ar bo xy la te s L C -M S /M S T ab le S 2 co nt in ue d . . . . 10 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 2 co nt in ue d M D L 3 M D L C la ss 1 C om m on na m e C he m ic al or ot he r na m e In st ru m en ta l an al ys is G C -M S G C -E C D T ox ap he ne T ox ap he ne T ec hn ic al st an da rd G C -E C N IM S < 0. 05 T ox ap he ne ho m ol og s H ex a T ec hn ic al st an da rd G C -E C N IM S < 0. 05 T ox ap he ne ho m ol og s H ep ta T ec hn ic al st an da rd G C -E C N IM S < 0. 05 T ox ap he ne ho m ol og s O ct a T ec hn ic al st an da rd G C -E C N IM S < 0. 05 T ox ap he ne ho m ol og s N on a T ec hn ic al st an da rd G C -E C N IM S < 0. 05 T ox ap he ne ho m ol og s D ec a T ec hn ic al st an da rd G C -E C N IM S < 0. 05 C hl or ob or na ne s2 P ar la r 11 -1 2 G C -E C N IM S < 0. 05 C hl or ob or na ne s P ar la r 15 G C -E C N IM S < 0. 02 C hl or ob or na ne s H ex -s ed G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 21 B 7- 49 9 G C -E C N IM S < 0. 02 C hl or ob or na ne s H ep -s ed G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 25 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 32 B 7- 51 5 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 26 B 8- 14 13 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 31 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 38 B 8- 78 9 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 39 B 8- 53 1 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 40 -4 1 B 8- 14 14 /1 94 5 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 42 B 8- 80 6 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 44 B 8- 22 29 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 50 B 9- 16 79 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 51 B 8- 78 6 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 56 B 9- 10 46 G C -E C N IM S < 0. 02 T ab le S 2 co nt in ue d . . . . ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 11 Ta bl e S 2 co nt in ue d M D L 3 M D L C la ss 1 C om m on na m e C he m ic al or ot he r na m e In st ru m en ta l an al ys is G C -M S G C -E C D C hl or ob or na ne s P ar la r 58 B 9- 71 5 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 59 B 9- 10 49 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 62 B 9- 10 25 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 63 B 9- 22 06 G C -E C N IM S < 0. 02 C hl or ob or na ne s P ar la r 69 B 10 -1 11 0 G C -E C N IM S < 0. 02 P B D E s B D E 17 di br om od ip he ny l et he r G C -E C N IM S < 0. 00 2 P B D E s B D E 28 /3 3 tr ib ro m od ip he ny l et he r G C -E C N IM S < 0. 00 2 P B D E s B D E 49 te tr ab ro m od ip he ny l et he r G C -E C N IM S < 0. 00 2 P B D E s B D E 47 te tr ab ro m od ip he ny l et he r G C -E C N IM S < 0. 03 5 P B D E s B D E 66 te tr ab ro m od ip he ny l et he r G C -E C N IM S < 0. 00 2 P B D E s B D E 10 0 pe nt ab ro m od ip he ny l et he r G C -E C N IM S < 0. 00 2 P B D E s B D E 99 pe nt ab ro m od ip he ny l et he r G C -E C N IM S < 0. 02 2 P B D E s B D E 85 pe nt ab ro m od ip he ny l et he r G C -E C N IM S < 0. 00 2 P B D E s B D E 15 4 he xa br om od ip he ny l et he r G C -E C N IM S < 0. 00 2 P B D E s B D E 15 3 he xa br om od ip he ny l et he r G C -E C N IM S < 0. 00 2 P B D E s B D E 13 8 he xa br om od ip he ny l et he r G C -E C N IM S < 0. 00 2 P B D E s B D E 18 3 he pt ab ro m od ip he ny l et he r G C -E C N IM S < 0. 00 2 P B D E s B D E 19 0 he pt ab ro m od ip he ny l et he r G C -E C N IM S < 0. 00 2 P B D E s B D E 20 9 de ca br om od ip he ny l et he r G C -E C N IM S < 0. 55 8 S um P B D E s ΣP B D E s O th er B F R s T B P -A E A lly l 2, 4, 6- tr ib ro m op he ny l et he r G C -E C N IM S < 0. 00 2 O th er B F R s pT B X te tr ab ro m ox yl en e G C -E C N IM S < 0. 00 2 O th er B F R s T B P -D B P E 2- B ro m oa lly l 2, 4, 6- tr ib ro m op he ny l et he r G C -E C N IM S < 0. 00 2 T ab le S 2 co nt in ue d . . . . 12 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 2 co nt in ue d M D L 3 M D L C la ss 1 C om m on na m e C he m ic al or ot he r na m e In st ru m en ta l an al ys is G C -M S G C -E C D O th er B F R s P B B e pe nt ab ro m ob en ze ne G C -E C N IM S < 0. 00 2 O th er B F R s T B C T te tr ab ro m o- o- ch lo ro to lu en e G C -E C N IM S < 0. 00 2 O th er B F R s P B To pe nt ab ro m ot ol ue ne G C -E C N IM S < 0. 00 2 O th er B F R s P B E B pe nt ab ro m oe th yl be nz en e G C -E C N IM S < 0. 00 2 O th er B F R s D P T E /T B P -D B P E 2, 3- D ib ro m op ro py l 2, 4, 6- tr ib ro m op he ny l et he r G C -E C N IM S < 0. 00 2 O th er B F R s H B B he xa br om ob en ze ne G C -E C N IM S < 0. 00 2 O th er B F R s B B -1 01 pe ne ta br om ob ip he ny l G C -E C N IM S < 0. 00 2 O th er B F R s P B B A pe nt ab ro m ob en zy l ac ry la te G C -E C N IM S < 0. 00 2 O th er B F R s E H T eB B 2- et hy l- 1- he xy l 2, 3, 4, 5- te tr ab ro m ob en zo at e G C -E C N IM S < 0. 00 2 O th er B F R s H B C D D he xa br om oc yc lo do de ca ne G C -E C N IM S < 0. 00 2 O th er B F R s B T B P E bi s( tr ib ro m op he no xy ) et ha ne G C -E C N IM S < 0. 00 2 O th er B F R s B E H T B P B is (2 -e th yl -1 -h ex yl ) te tr ab ro m op ht ha la te G C -E C N IM S < 0. 00 2 O C O sy n- D P S yn -D ec hl or an e G C -E C N IM S < 0. 00 2 O C O an ti- D P A nt i- D ec hl or an e G C -E C N IM S < 0. 00 2 O th er B F R s O B IN D O ct ab ro m ot ri m et hy lp he ny lin da ne G C -E C N IM S < 0. 00 2 1 O C O = ot he r ch lo ri na te d or ga ni c, O C P = or ga no ch lo ri ne pe st ic id e re la te d, P C B = po ly ch lo ri na te d bi ph en yl ,P B D E = po ly br om in at ed di ph en yl et he r, B F R = br om in at ed fl am e re ta rd an t. 2 N om en cl at ur e of ch lo ro bo rn an es ba se d on A nd re w s an d V et te r 19 95 . 3 M D L = m et ho d de te ct io n lim it = 3* S D of bl an k va lu es .“ na ” = no ta na ly se d. W he re no nd et ec tv al ue s w er e pr es en ti n bl an ks a“ < ” va lu es w er e us ed .T he se ar e in st ru m en t de te ct io n lim its ba se d on S /N of ap pr ox im at el y 10 :1 . ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 13 Table S3. Comparison of GC-MS and GC-ECD analysis of 3 moose liver samples. See Table S2 for full list analytes represented by each group. moose moose moose moose moose moose GC-MS ECD GC-MS ECD GC-MS ECD GEN-14 Gen-14 GEN-15 Gen-15r GEN-16 Gen-16 Target Analytes ng/g ww ng/g ww ng/g ww ng/g ww ng/g ww ng/g ww ΣDDT <0.002 <0.002 <0.002 <0.002 <0.002 0.01 ΣCHL 0.09 0.02 0.02 0.05 0.02 0.02 ΣHCH 0.11 0.13 0.07 0.11 0.07 0.12 HCB 0.19 0.17 0.24 0.12 0.20 0.15 ΣPCB 0.62 0.30 1.07 0.74 1.03 0.89 smono-di 0.22 0.06 0.44 0.11 0.21 0.13 Σ-tri 0.26 0.13 0.11 0.15 0.11 0.31 Σ-tetra 0.08 0.08 0.33 0.19 0.42 0.27 Σ-penta 0.05 <0.002 0.18 0.17 0.06 0.02 Σ-hexa 0.02 0.01 0.02 0.03 0.02 0.07 Σ-endosulfan 0.01 0.07 0.03 0.04 0.03 0.12 Table S4. Recoveries of internal standards during or prior to sample extraction. % recovery % recovery Compound GC-ECD analysis (n = 7) SD GC-MS analysis (n = 10) SD 1,3-DBB 84.7 3.5 85.6 23.9 1,3,5-TBB 80.2 3.8 112 29.2 1,2,4,5-TTBB 87.7 4.3 138 35.3 δ-HCH 79.0 5.2 110 39.3 Endrin Ketone 91.8 5.1 59.4 37.2 PCB 30 108 3.3 94.0 5.1 PCB 204 120 4.4 98.6 3.0 D16-gHBCDD1 84.1 17.7 13C-BDE-2091 67.4 15.8 13C12-PCB133 82.3 8.0 1HBCDD and BDE-209 were determined in n=7 samples by GC-NCIMS following GC-ECD analysis. 14 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 5. R ec ov er ie s of a O C P s an d P B D E st an da rd sp ik e (n = 1) du ri ng an al ys is of th e m oo se liv er sa m pl es . A na ly te % A na ly te % A na ly te % P B D E 17 95 H ex ac hl or ob ut ad ie ne 55 α- en do su lf an 97 P B D E 28 /3 3 10 1 1, 2, 4, 5- T T C B 70 ci s- C hl or da ne 72 P B D E 49 86 1, 2, 3, 4- T T C B 68 T ra ns -n on ac hl or 67 P B D E 71 96 P E C B 66 D ie ld ri n 79 P B D E 47 13 8 3, 4, 5- tr ic hl or ov er at ro le 87 p, p- D D E 67 P B D E 66 10 3 α- H C H 63 op -D D D 86 P B D E 10 0 11 6 β- H C H 78 E nd ri n 69 P B D E 99 13 3 H C B 71 b- E nd os ul fa n 77 P B D E 85 10 7 3, 4, 5, 6- te tr ac hl or ov er at ro le 76 p, p’ -D D D 72 P B D E 15 4 10 0 P en ta ch lo ro an is ol e 68 ci s- no na ch lo r 60 P B D E 15 3 10 4 γ- H C H (L in da ne ) 67 o, p’ -D D T 72 P B D E 13 8 85 H ep ta ch lo r 59 p, p’ -D D T 68 P B D E 18 3 92 A ld ri n 64 M et ho xy ch lo r 10 1 P B D E 19 0 92 H ep ta ch lo r E po xi de 79 M ir ex 75 P B D E 20 9 71 O xy ch lo rd an e 81 H B C D D 73 O ct ac hl or os ty re ne 62 B T B P E 78 tr an s- ch lo rd an e 62 D B D P E 11 7 o, p’ -D D E 72 ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 15 Ta bl e S 6. A ri th m et ic an d ge om et ri c m ea n co nc en tr at io ns of O C P /O C O s an d P C B s in m oo se liv er fr om th e D eh ch o an d S ou th S la ve re gi on s of th e N or th w es t Te rr ito ri es (n g/ g w et w ei gh ta nd lip id w ei gh t; n = 7 fo r ea ch re gi on ). “< ” va lu es ar e in st ru m en td et ec tio n lim its w he re ar ith m et ic m ea ns w er e < 0. 00 1- 0. 00 2 ng / g w w . D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A na ly te ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw % lip id 6. 3 6. 2 5. 3 7. 2 5. 7 5. 7 5. 0 6. 5 H ex ac hl or ob ut ad ie ne 0. 01 1 0. 01 0 0. 00 7 0. 01 4 0. 17 0. 17 0. 12 0. 24 0. 00 5 0. 00 4 0. 00 3 0. 01 1 0. 08 0 0. 07 4 0. 05 0 0. 16 1, 2, 4, 5- T et ra ch lo ro - be nz en e 0. 25 0. 24 0. 17 0. 30 4. 35 4. 22 2. 98 5. 50 0. 16 0. 14 0. 08 0. 35 2. 68 2. 52 1. 55 5. 38 1, 2, 3, 4- T et ra ch lo ro - be nz en e 0. 28 0. 27 0. 18 0. 35 4. 85 4. 68 3. 16 5. 87 0. 16 0. 15 0. 09 0. 36 2. 79 2. 64 1. 86 5. 54 P en ta ch lo ro be nz en e 0. 11 0. 03 7 0. 00 8 0. 32 1. 85 0. 59 0. 11 5. 91 0. 17 0. 16 0. 10 0. 45 3. 00 2. 74 1. 75 6. 85 H ex ac hl or ob en ze ne 0. 17 0. 17 0. 10 0. 24 2. 85 2. 67 1. 45 4. 33 0. 34 0. 26 0. 11 0. 93 6. 02 4. 64 1. 62 16 .6 3, 4, 5, 6- T et ra ch lo ro - ve ra tr ol e < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P en ta ch lo ro an is ol e 0. 04 0 0. 01 7 0. 00 5 0. 15 0. 70 0. 28 0. 07 2. 66 0. 02 9 0. 02 3 0. 00 4 0. 07 0. 52 0. 41 0. 08 1. 23 α- H C H 0. 04 1 0. 02 4 0. 00 6 0. 11 0. 70 0. 38 0. 09 1. 95 0. 05 6 0. 05 2 0. 03 1 0. 10 0. 99 0. 91 0. 49 1. 58 β- H C H 0. 10 0. 09 7 0. 05 9 0. 17 1. 66 1. 56 1. 11 2. 93 0. 07 9 0. 07 2 0. 04 0 0. 17 1. 38 1. 27 0. 71 2. 54 γ- H C H 0. 05 6 0. 03 5 0. 00 5 0. 14 0. 95 0. 57 0. 07 2. 48 0. 06 3 0. 05 8 0. 03 2 0. 13 1. 11 1. 02 0. 56 1. 92 P en ta ch lo ro ni tr ob en ze ne < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 02 H ep ta ch lo r < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 02 A ld ri n < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 02 D ac th al < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 02 oc ta ch lo ro st yr en e < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 02 H ep ta ch lo r E po xi de 0. 04 4 0. 03 3 0. 01 0 0. 08 0 0. 71 6 0. 53 0. 16 1. 47 0. 04 6 0. 03 7 0. 01 7 0. 15 0. 78 0. 65 0. 27 2. 23 T ab le S 6 co nt in ue d . . . . 16 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 6 co nt in ue d D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A na ly te ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw O xy ch lo rd an e 0. 03 3 0. 02 0 0. 00 4 0. 10 0. 51 0 0. 32 0. 06 4 1. 48 0. 03 9 0. 02 5 0. 00 9 0. 14 0. 66 0. 44 0. 15 2. 08 tr an s- ch lo rd an e < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 ci s- ch lo rd an e 0. 01 7 0. 01 1 < 0. 00 2 0. 03 8 0. 29 8 0. 17 4 0. 01 4 0. 69 7 0. 02 6 0. 02 2 0. 01 0 0. 06 5 0. 45 0. 38 3 0. 14 8 1. 00 0 tr an s- no na ch lo r 0. 03 1 0. 01 6 0. 00 4 0. 05 8 < 0. 02 < 0. 00 2 < 0. 02 D ie ld ri n < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 02 ci s- no na ch lo r < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 02 E nd ri n < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 02 α- E nd os ul fa n 0. 01 3 0. 01 0 0. 00 4 0. 02 4 0. 20 4 0. 16 0. 05 3 0. 35 0. 00 6 0. 00 4 < 0. 00 2 0. 02 5 0. 11 0. 06 6 0. 02 1 0. 50 β- E nd os ul fa n 0. 00 7 0. 00 6 0. 00 3 0. 01 5 0. 10 9 0. 09 2 0. 03 6 0. 22 0. 00 5 0. 00 5 0. 00 5 0. 00 5 0. 08 9 0. 08 8 0. 07 7 0. 10 E nd os ul fa n su lf at e 0. 02 6 0. 01 5 0. 00 3 0. 09 2 0. 43 4 0. 24 0. 05 9 1. 73 0. 00 5 0. 00 4 0. 00 2 0. 00 8 0. 08 6 0. 07 9 0. 03 4 0. 13 o, p’ -D D E < 0. 00 2 < 0. 02 < 0. 02 < 0. 2 p, p’ -D D E 0. 01 3 0. 01 3 0. 01 3 0. 01 4 < 0. 02 < 0. 02 < 0. 2 o, p’ -D D D < 0. 00 2 < 0. 02 < 0. 02 < 0. 2 p, p’ -D D D 0. 01 7 0. 01 7 0. 01 7 0. 01 7 < 0. 02 < 0. 02 < 0. 2 o, p’ -D D T < 0. 00 2 < 0. 02 < 0. 02 < 0. 2 p, p’ -D D T < 0. 00 2 < 0. 02 < 0. 02 < 0. 2 M et ho xy ch lo r < 0. 00 2 < 0. 02 < 0. 02 < 0. 2 M ir ex 0. 00 4 0. 00 2 < 0. 00 2 0. 01 4 0. 07 1 0. 04 0 0. 01 4 0. 26 0. 00 4 0. 00 3 < 0. 00 2 0. 01 3 0. 07 3 0. 04 9 0. 01 2 0. 26 T ox ap he ne 0. 84 0 0. 64 2 0. 27 8 2. 17 13 .6 10 .3 4. 37 30 .7 1. 06 0. 85 0 0. 16 7 1. 83 18 .8 15 .0 2. 57 32 .7 H ex ac hl or ob or na ne s 0. 02 6 0. 00 8 < 0. 00 2 0. 10 3 0. 41 9 0. 13 1 0. 01 4 1. 46 0. 01 2 0. 00 7 0. 00 5 0. 06 2 0. 23 5 0. 12 6 0. 07 7 1. 23 7 T ab le S 6 co nt in ue d . . . . ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 17 Ta bl e S 6 co nt in ue d D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A na ly te ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw H ep ta ch lo ro bo rn an es 0. 36 6 0. 04 7 0. 00 3 1. 50 5. 83 0. 75 3 0. 03 9 21 .3 0. 12 3 0. 03 9 0. 00 5 0. 49 4 2. 07 7 0. 68 2 0. 07 7 7. 72 O ct ac hl or ob or na ne s 0. 08 6 0. 03 9 < 0. 00 2 0. 25 1 1. 50 0. 62 9 0. 01 4 4. 72 0. 26 7 0. 17 4 0. 00 5 0. 43 1 4. 70 3. 07 0. 07 7 7. 70 N on ac hl or ob or na ne s 0. 31 8 0. 31 1 0. 22 5 0. 44 7 5. 10 4. 99 3. 54 6. 72 0. 48 1 0. 26 0 0. 00 5 1. 26 8. 50 4. 60 0. 07 7 22 .6 D ec ac hl or ob or na ne s 0. 04 8 0. 04 3 0. 01 5 0. 08 5 0. 77 0 0. 68 3 0. 21 1 1. 18 3 0. 18 6 0. 10 4 0. 01 0 0. 52 5 3. 43 1. 83 0. 20 8 10 .5 P 26 < 0. 02 < 0. 02 < 0. 2 P 50 < 0. 02 < 0. 02 < 0. 2 P 62 < 0. 02 < 0. 02 < 0. 2 P C B -1 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 02 P C B -3 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 02 P C B 4/ 10 0. 00 5 0. 00 5 0. 00 4 0. 00 9 0. 08 6 0. 08 3 0. 05 1 0. 13 0. 02 8 0. 00 8 0. 00 5 0. 19 0. 50 2 0. 14 0. 07 7 3. 39 P C B 7/ 9 0. 03 6 0. 02 0 0. 00 5 0. 06 4 0. 55 3 0. 32 8 0. 07 9 1. 07 0. 00 5 0. 00 5 0. 00 5 0. 00 5 0. 08 9 0. 08 8 0. 07 7 0. 10 0 P C B 6 0. 00 6 0. 00 6 0. 00 4 0. 00 8 0. 09 2 0. 09 0 0. 06 0 0. 13 0. 00 5 0. 00 5 0. 00 5 0. 00 5 0. 08 9 0. 08 8 0. 07 7 0. 10 0 P C B 8/ 5 0. 01 6 0. 01 3 0. 00 5 0. 02 6 0. 21 3 0. 15 0 0. 02 0 0. 37 0. 00 5 0. 00 5 0. 00 5 0. 00 5 0. 08 9 0. 08 8 0. 07 7 0. 10 0 P C B 12 /1 3 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 02 P C B 15 0. 11 7 0. 01 1 < 0. 00 2 0. 42 0 2. 05 0. 17 2 0. 01 4 7. 71 0. 10 6 0. 04 8 0. 00 5 0. 33 0 1. 90 7 0. 85 5 0. 08 9 5. 89 P C B 19 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 18 0. 04 5 0. 04 5 0. 03 9 0. 05 3 0. 68 0. 67 6 0. 56 6 0. 76 < 0. 00 2 < 0. 02 P C B 17 0. 03 8 0. 02 5 0. 00 5 0. 09 0 0. 63 0. 39 6 0. 08 9 1. 65 0. 02 1 0. 01 5 0. 00 5 0. 06 0 0. 36 2 0. 25 8 0. 07 7 0. 92 P C B 27 /2 4 0. 00 3 0. 00 2 < 0. 00 2 0. 00 4 0. 04 4 0. 03 7 0. 01 6 0. 07 1 < 0. 00 2 < 0. 02 P C B 16 /3 2 0. 00 9 0. 00 4 0. 00 0 0. 01 8 0. 13 8 0. 06 0 0. 00 4 0. 26 < 0. 00 2 < 0. 02 T ab le S 6 co nt in ue d . . . . 18 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 6 co nt in ue d D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A na ly te ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw P C B 26 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 25 0. 00 6 0. 00 5 0. 00 2 0. 01 3 0. 11 3 0. 07 6 0. 03 3 0. 25 2 < 0. 00 2 < 0. 02 P C B 31 /2 8 0. 02 9 0. 01 5 0. 00 5 0. 08 5 0. 47 0. 23 5 0. 07 9 1. 60 0. 00 5 0. 00 5 0. 00 5 0. 00 5 0. 08 9 0. 08 8 0. 07 7 0. 10 0 P C B 20 /3 3/ 21 0. 04 3 0. 01 4 0. 00 4 0. 24 0. 74 0. 21 7 0. 05 7 4. 26 0. 06 8 0. 01 5 0. 00 5 0. 43 5 1. 06 1 0. 25 9 0. 07 8 6. 69 P C B 22 0. 00 2 < 0. 00 2 < 0. 00 2 0. 00 3 0. 02 7 0. 02 0 0. 01 0 0. 04 4 < 0. 00 2 < 0. 02 P C B 37 0. 00 3 0. 00 2 < 0. 00 2 0. 00 5 0. 04 6 0. 03 2 0. 01 4 0. 09 2 0. 01 8 0. 00 7 0. 00 5 0. 11 0 0. 35 1 0. 13 0 0. 07 7 2. 20 P C B 53 0. 00 4 0. 00 4 0. 00 4 0. 00 4 0. 08 3 0. 08 3 0. 08 3 0. 08 3 < 0. 00 2 < 0. 02 P C B 45 0. 01 1 0. 01 0 0. 00 8 0. 01 3 0. 18 0. 17 0 0. 12 0 0. 24 < 0. 00 2 < 0. 02 P C B 46 0. 00 6 0. 00 5 0. 00 3 0. 00 9 0. 10 0. 08 4 0. 04 4 0. 16 < 0. 00 2 < 0. 02 P C B 73 /5 2 0. 07 9 0. 02 0 < 0. 00 2 0. 27 1. 33 0. 33 0. 01 4 4. 25 0. 00 5 0. 00 5 0. 00 5 0. 00 5 0. 08 9 0. 08 8 0. 07 7 0. 10 0 P C B 43 /4 9 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 48 /4 7/ 75 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 44 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 59 /4 2 0. 01 9 0. 00 7 < 0. 00 2 0. 03 6 0. 29 8 0. 11 8 0. 01 6 0. 67 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 8 0. 01 8 0. 01 5 0. 02 0 P C B 71 /4 1/ 68 /6 4 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 63 0. 00 3 0. 00 3 0. 00 3 0. 00 3 0. 05 6 0. 05 6 0. 05 6 0. 06 < 0. 00 2 < 0. 02 P C B 74 /6 1 0. 01 0 0. 00 2 0. 00 0 0. 06 0 0. 16 8 0. 04 0 0. 00 7 0. 94 0. 00 5 0. 00 5 0. 00 5 0. 00 5 0. 08 9 0. 08 8 0. 07 7 0. 10 0 P C B 70 /7 6 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 80 /6 6 0. 06 8 0. 01 5 < 0. 00 2 0. 13 1. 05 4 0. 24 0. 01 6 2. 49 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 8 0. 01 8 0. 01 5 0. 02 0 P C B 56 /6 0 0. 04 1 0. 00 8 < 0. 00 2 0. 12 0. 70 6 0. 12 0. 01 4 2. 20 0. 04 6 0. 03 1 0. 00 5 0. 14 0. 81 0. 54 6 0. 07 7 2. 50 T ab le S 6 co nt in ue d . . . . ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 19 Ta bl e S 6 co nt in ue d D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A na ly te ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw P C B 81 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 95 /9 3 0. 00 2 0. 00 2 < 0. 00 2 0. 00 5 0. 03 7 0. 02 6 0. 01 4 0. 09 2 0. 00 5 0. 00 5 0. 00 5 0. 00 5 0. 08 9 0. 08 8 0. 07 7 0. 10 0 P C B 91 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 92 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 84 /9 0/ 10 1/ 89 0. 01 8 0. 00 3 < 0. 00 2 0. 10 0 0. 28 6 0. 04 0 0. 01 4 1. 83 0. 00 5 0. 00 5 0. 00 5 0. 00 5 0. 08 9 0. 08 8 0. 07 7 0. 10 0 P C B 99 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 83 /1 08 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 97 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 86 /1 11 /1 25 /1 17 /8 7/ 11 6/ 11 5 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 12 0/ 85 0. 02 6 0. 00 8 < 0. 00 2 0. 07 5 0. 45 2 0. 12 2 0. 01 4 1. 38 0. 02 8 0. 02 5 0. 01 5 0. 04 0 0. 48 0. 45 0. 23 0. 71 P C B 11 0 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 82 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 10 7/ 10 9 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 12 3 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 11 8/ 10 6 0. 00 6 0. 00 6 0. 00 6 0. 00 6 0. 08 7 0. 08 7 0. 08 7 0. 08 7 < 0. 00 2 < 0. 02 P C B 11 4 0. 00 0 0. 00 0 0. 00 0 0. 00 0 0. 00 3 0. 00 3 0. 00 3 0. 00 3 < 0. 00 2 < 0. 02 P C B 10 5/ 12 7 0. 00 2 < 0. 00 2 < 0. 00 2 0. 00 6 0. 03 1 0. 02 1 0. 01 4 0. 12 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 8 0. 01 8 0. 01 5 0. 02 0 P C B 12 6 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 15 1 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 T ab le S 6 co nt in ue d . . . . 20 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 6 co nt in ue d D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A na ly te ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw P C B 13 5/ 14 4 0. 00 8 0. 00 2 < 0. 00 2 0. 05 1 0. 14 9 0. 02 8 0. 01 4 0. 95 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 8 0. 01 8 0. 01 5 0. 02 0 P C B 13 9/ 14 9 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 13 1/ 16 5/ 14 2/ 14 6 0. 00 3 0. 00 2 < 0. 00 2 0. 00 5 0. 05 1 0. 03 7 0. 01 4 0. 09 2 0. 00 6 0. 00 5 0. 00 5 0. 01 0 0. 10 1 0. 09 6 0. 07 7 0. 20 0 P C B 15 3 0. 00 3 0. 00 2 < 0. 00 2 0. 00 5 0. 05 5 0. 04 0 0. 01 4 0. 09 2 0. 01 4 0. 00 7 0. 00 5 0. 08 0 0. 26 0. 12 5 0. 07 7 1. 42 9 P C B 13 2/ 16 8 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 14 1 0. 00 3 0. 00 3 0. 00 3 0. 00 3 0. 04 8 0. 04 8 0. 04 8 0. 04 8 < 0. 00 2 < 0. 02 P C B 13 7 0. 00 3 0. 00 2 < 0. 00 2 0. 00 5 0. 05 5 0. 04 0 0. 01 4 0. 09 2 0. 00 6 0. 00 5 0. 00 5 0. 01 0 0. 10 0. 09 6 0. 07 7 0. 17 9 P C B 16 3/ 16 4/ 13 8 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 15 8/ 16 0 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 6 0. 01 6 0. 01 6 0. 01 6 < 0. 00 2 < 0. 02 P C B 12 9 0. 00 3 0. 00 3 0. 00 3 0. 00 3 0. 05 8 0. 05 8 0. 05 8 0. 05 8 < 0. 00 2 < 0. 02 P C B 15 9 0. 00 5 0. 00 3 < 0. 00 2 0. 01 2 0. 07 6 0. 04 9 0. 01 6 0. 22 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 8 0. 01 8 0. 01 5 0. 02 0 P C B 12 8/ 16 7 0. 00 5 0. 00 5 0. 00 5 0. 00 5 0. 08 5 0. 08 5 0. 08 5 0. 08 5 < 0. 00 2 < 0. 02 P C B 15 6 0. 00 2 0. 00 2 < 0. 00 2 0. 00 7 0. 03 8 0. 02 5 0. 00 8 0. 14 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 8 0. 01 8 0. 01 5 0. 02 0 P C B 15 7 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 16 9 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 18 2/ 18 7 0. 00 3 0. 00 2 < 0. 00 2 0. 01 1 0. 04 5 0. 02 5 0. 01 4 0. 20 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 8 0. 01 8 0. 01 5 0. 02 0 P C B 18 3 0. 00 3 0. 00 2 < 0. 00 2 0. 01 4 0. 05 9 0. 02 9 0. 01 4 0. 26 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 8 0. 01 8 0. 01 5 0. 02 0 P C B 17 4/ 18 1 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 17 7 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 17 1 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 T ab le S 6 co nt in ue d . . . . ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 21 Ta bl e S 6 co nt in ue d D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A na ly te ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw P C B 17 2/ 19 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 6 0. 01 6 0. 01 4 0. 02 0 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 8 0. 01 8 0. 01 5 0. 02 0 P C B 18 0 0. 00 3 0. 00 2 < 0. 00 2 0. 00 7 0. 04 1 0. 02 7 0. 01 4 0. 11 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 8 0. 01 8 0. 01 5 0. 02 0 P C B 19 3 0. 00 9 0. 00 2 < 0. 00 2 0. 05 8 0. 17 0. 03 4 0. 01 4 1. 08 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 8 0. 01 8 0. 01 5 0. 02 0 P C B 19 1 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 17 0/ 19 0 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 1 0. 01 1 0. 01 1 0. 01 1 < 0. 00 2 < 0. 02 P C B -2 02 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 19 9 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 19 6/ 20 3 0. 00 2 0. 00 2 < 0. 00 2 0. 00 5 0. 03 6 0. 03 0 0. 01 6 0. 09 0 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 8 0. 01 8 0. 01 5 0. 02 0 P C B 19 5 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 19 4 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 00 2 0. 02 2 0. 02 1 0. 01 6 0. 03 3 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 8 0. 01 8 0. 01 5 0. 02 0 P C B 20 5 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 20 8 0. 00 5 0. 00 2 < 0. 00 2 0. 03 2 0. 09 9 0. 02 7 0. 00 9 0. 59 3 < 0. 00 2 < 0. 00 2 < 0. 00 2 < 0. 00 2 0. 01 8 0. 01 8 0. 01 5 0. 02 0 P C B 20 7 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 20 6 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 P C B 20 9 < 0. 00 2 < 0. 02 < 0. 00 2 < 0. 00 2 < 0. 02 22 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 7. A ri th m et ic an d ge om et ri c m ea n co nc en tr at io ns of br om in at ed an d ch lo ri na te d fl am e re ta rd an ts (n g/ g w et w t an d ng /g lip id w t, n = 7) .“ < ” va lu es ar e in st ru m en t de te ct io n lim its w he re ar ith m et ic m ea ns w er e < 0. 00 1- 0. 00 2 ng /g w w . “n a” in di ca te s no t av ai la bl e. D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A na ly te ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw ng / g w w ng / g w w ng / g w w ng / g w w ng / g lw ng / g lw ng / g lw ng / g lw B D E 17 < 0. 00 2 0. 00 1 < 0. 00 2 0. 00 2 0. 01 1 0. 01 1 < 0. 00 7 0. 02 2 < 0. 00 2 0. 00 1 < 0. 00 2 0. 00 2 0. 00 9 0. 00 9 < 0. 00 8 0. 01 0 B D E 28 /3 3 0. 00 3 0. 00 1 0. 00 1 0. 01 0 0. 04 6 0. 02 3 0. 00 7 0. 14 0. 00 1 0. 00 1 0. 00 1 0. 00 1 0. 00 9 0. 00 9 0. 00 8 0. 01 0 B D E 47 0. 12 7 0. 03 7 0. 00 5 0. 51 3 2. 17 0. 58 5 0. 06 9 9. 40 0. 05 7 0. 04 8 0. 02 3 0. 13 3 0. 99 8 0. 84 5 0. 35 3 2. 37 0 B D E 49 0. 00 4 0. 00 2 0. 00 1 0. 01 2 0. 07 0. 03 0. 00 7 0. 22 0. 00 1 0. 00 1 0. 00 1 0. 00 1 0. 00 9 0. 00 9 0. 00 8 0. 01 0 B D E 66 0. 00 2 0. 00 1 0. 00 0 0. 00 5 0. 03 2 0. 01 8 0. 00 4 0. 07 6 0. 00 1 0. 00 1 0. 00 1 0. 00 1 0. 00 9 0. 00 9 0. 00 8 0. 01 0 B D E 85 0. 00 6 0. 00 1 0. 00 1 0. 03 8 0. 11 1 0. 02 1 0. 00 7 0. 68 9 0. 00 4 0. 00 1 0. 00 1 0. 01 5 0. 06 3 0. 02 0 0. 00 8 0. 23 1 B D E 99 0. 14 8 0. 03 6 0. 00 5 0. 66 8 2. 56 0. 58 1 0. 06 9 12 .2 6 0. 08 1 0. 05 4 0. 01 9 0. 26 6 1. 41 0. 95 1 0. 30 0 4. 75 B D E 10 0 0. 02 6 0. 00 8 0. 00 1 0. 13 4 0. 46 6 0. 12 9 0. 00 7 2. 47 0. 01 8 0. 01 2 0. 00 4 0. 05 3 0. 30 4 0. 21 8 0. 06 3 0. 94 5 B D E 13 8 < 0. 00 2 0. 11 1 0. 02 1 0. 00 7 0. 69 < 0. 00 2 0. 06 3 0. 02 0 0. 00 8 0. 23 B D E 15 3 0. 01 4 0. 00 7 0. 00 1 0. 06 1 0. 25 0 0. 10 6 0. 00 8 1. 12 0. 00 8 0. 00 3 0. 00 1 0. 02 5 0. 13 5 0. 05 5 0. 00 8 0. 38 9 B D E 15 4 0. 01 0 0. 00 4 0. 00 1 0. 04 8 0. 18 1 0. 06 3 0. 00 8 0. 88 3 0. 00 5 0. 00 1 0. 00 1 0. 02 0 0. 08 6 0. 02 2 0. 00 8 0. 32 0 B D E 18 3 < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 B D E 19 0 < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 B D E 20 9 0. 15 0. 07 7 0. 00 4 0. 54 3 2. 26 1. 24 0. 07 7 7. 69 na na T B P -A E < 0. 00 2 < 0. 01 0. 02 7 0. 00 3 < 0. 00 2 0. 20 0 0. 49 1 0. 04 5 0. 00 8 3. 57 T B P - D B P E < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 B E H T B P < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 T ab le S 7 co nt in ue d . . . . ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 23 Ta bl e S 7 co nt in ue d D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o D eh ch o S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A ri th M ea n G M m in m ax A na ly te ng / g w w ng / g w w ng / g w w ng / g w w ng /g lw ng /g lw ng /g lw ng /g lw ng / g w w ng / g w w ng / g w w ng / g w w ng / g lw ng / g lw ng / g lw ng / g lw B T B P E < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 D P T E < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 E H T eB B < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 H B B < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 O B IN D < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 P B B A < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 P B B e < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 P B E B < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 P B To < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 pT B X < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 sy n- D P < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 an ti- D P < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 T B C T < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 H B C D < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 B B -1 01 < 0. 00 2 < 0. 01 < 0. 00 2 < 0. 01 24 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S8 . A ri th m et ic an d ge om et ri c m ea n co nc en tr at io ns of in di vi du al PF A Ss in m oo se liv er sa m pl es (n g/ g w w ). D eh ch o D eh ch o D eh ch o D eh ch o S ou th S la ve S ou th S la ve S ou th S la ve S ou th S la ve ng /g w w ng /g w w ng /g w w ng /g w w ng /g w w ng /g w w ng /g w w ng /g w w A na ly te A M G M m in m ax A M G M m in m ax P F B A < 0. 16 < 0. 16 P F P eA < 1. 2 < 1. 2 P F H xA < 0. 15 < 0. 15 P F H pA < 0. 00 5 < 0. 00 5 P F O A 0. 06 9 0. 06 7 0. 05 6 0. 11 3 0. 09 0 0. 08 5 0. 05 0 0. 13 8 P F N A 0. 25 5 0. 24 3 0. 17 4 0. 44 8 0. 18 8 0. 17 1 0. 08 0 0. 37 0 P F D A 0. 24 0 0. 20 4 0. 07 8 0. 60 1 0. 15 9 0. 14 9 0. 06 9 0. 24 1 P F U nA 0. 16 7 0. 15 2 0. 09 6 0. 27 5 0. 09 4 0. 09 0 0. 05 8 0. 13 8 P F D oA 0. 00 8 0. 00 5 0. 00 3 0. 02 0 0. 01 7 0. 01 6 0. 00 8 0. 02 3 P F T ri A 0. 01 1 0. 00 6 0. 00 3 0. 03 2 0. 02 3 0. 02 1 0. 01 0 0. 04 1 P F TA 0. 00 4 0. 00 3 < 0. 00 3 0. 01 5 0. 01 2 0. 00 8 0. 00 3 0. 04 5 P F H xD A 0. 00 4 0. 00 3 0. 00 3 0. 01 0 0. 04 9 0. 03 2 0. 01 0 0. 17 3 P F B S 0. 05 5 0. 05 2 0. 02 6 0. 08 7 0. 02 9 0. 01 8 0. 00 3 0. 05 1 P F H xS 0. 06 3 0. 05 7 0. 02 2 0. 10 6 0. 01 2 0. 00 7 0. 00 3 0. 05 4 P F H pS < 0. 00 5 < 0. 00 5 P F O S 0. 43 7 0. 37 7 0. 21 0 0. 99 3 0. 25 2 0. 24 4 0. 17 4 0. 33 5 P F O S _L in ea r 0. 18 9 0. 18 9 0. 17 3 0. 20 2 0. 18 3 0. 17 2 0. 08 8 0. 26 2 P F D S 0. 00 9 0. 00 5 0. 00 3 0. 03 4 < 0. 00 5 P F O S A 0. 00 8 0. 00 4 0. 00 3 0. 03 0 0. 01 2 0. 00 8 0. 00 3 0. 03 2 ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 25 Ta bl e S 9A . P ea rs on co rr el at io n m at ri x fo r m aj or ha lo ge na te d or ga ni c co nt am in an ts in m oo se liv er . C or re la tio n co ef fi ci en ts in bo ld ar e si gn if ic an t at P < 0. 05 . A ge % lip id L og ΣP F C A L og ΣP F S A L og Σ 1 3 P B D E L og ΣP C B L og ΣE nd o- su lf an L og T ox a- ph en e L og ΣD D T L og ΣC H L L og ΣH C H L og ΣC B Z L og ΣM on o_ D i C B L og ΣT ri -C B L og ΣT et ra - C B L og Σp en ta - C B A ge 1 % lip id 0. 26 6 1 L og ΣP F C A 0. 24 8 0. 40 6 1 L og ΣP F S A 0. 07 6 0. 59 0 0. 69 9 1 L og Σ 1 3 P B D E 0. 18 7 0. 34 1 0. 01 8 0. 13 8 1 L og ΣP C B 0. 28 5 0. 04 2 � 0. 00 1 � 0. 03 3 0. 46 0 1 L og ΣE nd o- su lf an 0. 00 6 0. 34 1 0. 32 8 0. 61 8 0. 00 5 0. 30 9 1 L og T ox a- ph en e � 0. 28 8 � 0. 15 2 � 0. 49 8 � 0. 50 5 � 0. 20 1 � 0. 04 6 � 0. 05 4 1 L og ΣD D T � 0. 36 8 0. 42 � 0. 03 8 0. 41 8 � 0. 08 6 0. 10 3 0. 62 3 0. 26 8 1 L og ΣC H L 0. 10 7 0. 10 3 0. 05 � 0. 09 6 0. 60 2 0. 35 8 � 0. 42 3 � 0. 31 4 � 0. 50 5 1 L og ΣH C H 0. 14 9 � 0. 24 0 � 0. 25 1 � 0. 40 5 � 0. 01 1 � 0. 29 4 � 0. 79 � 0. 19 5 � 0. 69 8 0. 51 4 1 L og ΣC B Z 0. 38 � 0. 49 6 � 0. 16 3 � 0. 55 5 0. 03 2 0. 05 2 � 0. 62 2 � 0. 15 4 � 0. 82 1 0. 45 7 0. 75 1 L og ΣM on o_ D i- C B 0. 22 7 � 0. 55 6 � 0. 37 1 � 0. 82 9 � 0. 32 4 0. 14 7 � 0. 51 4 0. 21 2 � 0. 55 9 0. 08 9 0. 48 8 0. 67 1 1 L og ΣT ri -C B � 0. 02 8 � 0. 48 1 � 0. 11 8 � 0. 58 3 � 0. 32 5 0. 03 6 � 0. 64 3 � 0. 03 8 � 0. 50 2 0. 32 1 0. 59 4 0. 59 2 0. 78 1 1 L og ΣT et ra -C B 0. 48 5 � 0. 31 9 � 0. 42 6 � 0. 60 5 0. 15 4 0. 61 3 � 0. 28 5 0. 09 1 � 0. 26 7 0. 20 1 0. 23 3 0. 54 6 0. 70 9 0. 45 9 1 L og Σp en ta -C B 0. 51 8 � 0. 38 4 � 0. 01 8 � 0. 33 9 � 0. 30 3 0. 22 3 � 0. 20 2 � 0. 22 9 � 0. 42 6 0. 05 9 0. 34 4 0. 62 8 0. 68 1 0. 66 4 0. 66 7 1 L og Σh ex a- C B � 0. 29 4 0. 42 1 0. 36 5 0. 57 6 � 0. 19 6 � 0. 08 1 0. 55 5 0. 27 7 0. 68 6 � 0. 30 9 � 0. 65 9 � 0. 71 7 � 0. 66 2 � 0. 45 3 � 0. 58 8 � 0. 41 6 26 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 9B .P ea rs on co rr el at io n m at ri x fo r se le ct ed in di vi du al ha lo ge na te d or ga ni c co nt am in an ts in m oo se liv er .C or re la tio n co ef fi ci en ts in bo ld ar e si gn if ic an ta t P < 0. 05 . A G E % lip id L og H C B D L og 12 45 - Te C B z L og 12 34 - Te C B z L og P eC B z L og H C B L og P C A L og αH C H L og βH C H L og γH C H L og he pt a- ch lo r ep ox id e L og O xy ch lo rd an e A G E 1. 00 0 % lip id 0. 26 6 1. 00 0 L og H C B D 0. 03 5 0. 56 9 1. 00 0 L og 12 45 _T eC B z 0. 34 1 � 0. 51 3 � 0. 60 8 1. 00 0 L og 12 34 _T eC B z 0. 34 7 � 0. 51 3 � 0. 59 9 1. 00 0 1. 00 0 L og P eC B z 0. 34 2 � 0. 45 7 � 0. 55 2 0. 97 2 0. 97 2 1. 00 0 L og H C B 0. 10 3 � 0. 42 6 � 0. 51 0 0. 46 7 0. 45 8 0. 47 6 1. 00 0 L og P C A 0. 43 6 � 0. 29 8 � 0. 52 9 0. 77 8 0. 77 0 0. 82 8 0. 54 3 1. 00 0 L og αH C H 0. 24 0 � 0. 58 7 � 0. 52 0 0. 92 0 0. 91 9 0. 87 2 0. 59 1 0. 66 4 1. 00 0 L og βH C H � 0. 17 8 0. 40 8 0. 41 6 � 0. 13 0 � 0. 12 8 � 0. 11 7 � 0. 34 1 � 0. 21 7 � 0. 17 0 1. 00 0 L og γH C H 0. 13 9 � 0. 35 1 � 0. 55 1 0. 64 1 0. 64 1 0. 69 7 0. 41 4 0. 53 7 0. 51 2 0. 15 5 1. 00 0 L og he pt ac hl or ep ox id e 0. 32 9 0. 02 1 � 0. 05 9 0. 53 1 0. 53 4 0. 54 2 0. 11 6 0. 55 6 0. 32 7 0. 17 2 0. 30 2 1. 00 0 L og O xy ch lo rd an e � 0. 03 8 0. 26 5 � 0. 02 7 0. 11 4 0. 11 2 0. 21 2 0. 33 5 0. 30 8 0. 14 6 0. 28 2 0. 36 4 0. 30 6 1. 00 0 L og ci s- ch lo rd an e 0. 20 1 � 0. 59 4 � 0. 37 6 0. 72 1 0. 72 3 0. 67 9 0. 28 3 0. 46 2 0. 63 3 � 0. 28 2 0. 38 3 0. 58 6 � 0. 23 8 L og α- E nd os ul fa n 0. 12 4 0. 36 9 0. 32 7 � 0. 32 0 � 0. 31 4 � 0. 28 3 � 0. 45 7 0. 02 3 � 0. 49 4 � 0. 02 5 � 0. 40 6 0. 38 0 � 0. 14 0 L og en do su lf an su lf at e 0. 07 2 0. 43 2 0. 63 5 � 0. 72 9 � 0. 72 6 � 0. 70 0 � 0. 49 9 � 0. 52 8 � 0. 65 0 � 0. 18 0 � 0. 69 3 � 0. 43 6 � 0. 44 4 L og m ir ex � 0. 10 2 � 0. 46 4 0. 03 6 0. 18 6 0. 19 7 0. 12 7 � 0. 04 7 � 0. 27 4 0. 32 1 0. 13 4 0. 18 2 � 0. 15 4 � 0. 33 7 L og B D E 47 0. 46 2 � 0. 22 5 � 0. 31 1 0. 63 1 0. 62 8 0. 63 2 0. 33 3 0. 73 6 0. 44 8 � 0. 06 8 0. 42 5 0. 83 0 0. 25 8 L og P F N A 0. 14 8 0. 45 2 0. 54 8 � 0. 31 1 � 0. 30 6 � 0. 28 5 � 0. 49 8 � 0. 33 6 � 0. 43 7 0. 41 5 � 0. 07 1 0. 33 3 � 0. 04 0 L og P F D A 0. 39 1 0. 33 9 0. 29 5 � 0. 21 1 � 0. 20 8 � 0. 26 4 � 0. 01 0 � 0. 23 4 � 0. 24 6 0. 15 2 � 0. 10 0 0. 29 6 � 0. 05 9 L og P F U N A 0. 02 5 0. 50 2 0. 62 5 � 0. 67 7 � 0. 67 1 � 0. 69 4 � 0. 43 3 � 0. 66 5 � 0. 70 5 0. 08 5 � 0. 61 7 � 0. 01 0 � 0. 28 2 L og P F B S � 0. 00 5 0. 24 5 0. 58 9 � 0. 18 5 � 0. 17 0 � 0. 17 3 � 0. 47 5 � 0. 24 6 � 0. 20 1 0. 74 3 � 0. 10 1 0. 21 3 0. 00 1 L og P F H X S 0. 19 3 0. 35 9 0. 67 8 � 0. 40 8 � 0. 39 7 � 0. 33 6 � 0. 16 4 � 0. 14 9 � 0. 28 1 � 0. 16 0 � 0. 62 7 0. 05 3 0. 15 1 L og P F O S � 0. 00 8 0. 55 5 0. 61 1 � 0. 70 5 � 0. 70 4 � 0. 71 5 � 0. 23 6 � 0. 59 0 � 0. 70 2 0. 36 8 � 0. 38 1 � 0. 02 3 � 0. 08 7 ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 27 L og O xy ch lo rd an e L og ci s- ch lo rd an e L og αE nd os lf an L og en do su lf an su lf at e L og B D E 47 L og P F N A L og P F D A L og P F U N A L og P F B S L og P F H X S A G E % lip id L og H C B D L og 12 45 _T C B Z L og 12 34 _T C B Z L og P eC B Z L og H C B L og P C A L og αH C H L og βH C H L og γH C H L og he pt ac hl or ep ox id e L og O xy ch lo rd an e L og ci s- ch lo rd an e 1 L og α- E nd os ul fa n � 0. 07 6 1 L og en do su lf an su lf at e � 0. 35 3 0. 45 9 1 L og m ir ex 0. 44 4 � 0. 47 1 � 0. 04 4 1 L og B D E 47 0. 63 5 0. 11 9 � 0. 53 1 � 0. 08 7 1 L og P F N A 0. 15 1 0. 30 2 0. 34 6 0. 23 8 0. 18 1 L og P F D A 0. 18 5 0. 12 8 0. 21 2 0. 22 8 0. 29 3 0. 73 1 L og P F U N A � 0. 15 2 0. 38 2 0. 60 7 0. 05 3 � 0. 16 1 0. 69 5 0. 71 7 1 L og P F B S � 0. 16 9 0. 32 3 0. 08 5 0. 27 6 � 0. 04 4 0. 38 0. 18 2 0. 23 7 1 L og P F H X S � 0. 26 7 0. 48 2 0. 55 2 � 0. 20 9 � 0. 08 5 0. 12 6 0. 11 9 0. 41 9 0. 27 2 1 L og P F O S � 0. 31 7 0. 31 3 0. 48 7 � 0. 01 5 � 0. 18 5 0. 65 8 0. 75 9 0. 84 2 0. 36 9 0. 25 7 28 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 9C .P ea rs on co rr el at io n m at ri x fo r se le ct ed P C B co ng en er s (c o- el ut er s re po rt ed as si ng le G C pe ak s) in m oo se liv er . C or re la tio n co ef fi ci en ts in bo ld ar e si gn if ic an t at P < 0. 05 . A ge % L ip id L og C B 15 L og C B 31 _2 8 L og C B 73 _5 2 L og C B 56 _6 0 L og C B 10 5_ 12 7 L og C B 15 3 L og C B 15 6 L og C B 18 0 A ge 1 % L ip id 0. 26 6 1 L og C B 15 � 0. 45 5 0. 06 6 1 L og C B 31 _2 8 0. 38 8 � 0. 42 4 � 0. 30 9 1 L og C B 73 _5 2 � 0. 37 2 0. 35 1 0. 15 5 � 0. 72 1 1 L og C B 56 _6 0 0. 39 5 � 0. 05 4 � 0. 28 5 0. 24 9 0. 21 3 1 L og C B 10 5_ 12 7 0. 42 4 � 0. 53 2 � 0. 18 7 0. 92 2 � 0. 77 0 0. 24 3 1 L og C B 15 3 � 0. 23 9 � 0. 22 3 � 0. 00 3 � 0. 36 7 0. 64 6 0. 22 0 � 0. 31 1 1 L og C B 15 6 0. 12 9 � 0. 57 2 0. 25 0 0. 32 6 � 0. 46 6 0. 04 3 0. 62 5 0. 01 4 1 L og C B 18 0 � 0. 11 3 0. 02 1 � 0. 02 5 � 0. 50 0 0. 47 4 � 0. 12 3 � 0. 49 2 0. 79 6 � 0. 19 4 1 L og C B 19 6_ 20 3 � 0. 06 3 0. 13 9 � 0. 21 2 � 0. 53 8 0. 43 8 � 0. 18 1 � 0. 55 8 0. 61 2 � 0. 29 9 0. 88 6 ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 29 Ta bl e S 10 A . C om pa ri so n of m ea n co nc en tr at io ns (l og ng /g w et w t) of m aj or ha lo ge na te d or ga ni cs in m oo se liv er fr om th e D eh ch o an d S ou th S la ve (S S R ) re gi on s us in g th e S tu de nt s t- te st as su m in g se pa ra te va ri an ce ; si gn if ic an t va lu es ar e bo ld ed (P < 0. 05 ). V ar ia bl e R eg io n n M ea n S ta nd D ev M ea n D if fe re nc e L ow er 95 % L im it U pp er 95 % L im it t df p- V al ue L og ΣP F C A D eh ch o 7 � 0. 15 2 0. 16 2 0. 05 7 � 0. 10 5 0. 21 8 0. 78 3 10 .1 29 0. 45 2 S S R 7 � 0. 20 9 0. 10 2 L og ΣP F S A D eh ch o 7 � 0. 28 4 0. 18 9 0. 24 5 0. 06 1 0. 42 9 3. 0 9. 38 2 0. 01 4 S S R 7 � 0. 52 9 0. 10 5 L og ΣP B D E D eh ch o 7 � 0. 58 9 0. 51 6 0. 35 4 � 0. 17 5 0. 88 4 1. 47 4 10 .9 23 0. 16 9 S S R 7 � 0. 94 3 0. 37 3 L og ΣP C B D eh ch o 7 � 0. 21 7 0. 17 7 0. 24 0. 01 6 0. 46 5 2. 34 1 11 .7 42 0. 03 8 S S R 7 � 0. 45 8 0. 20 6 L og ΣE nd os ul fa n D eh ch o 7 � 1. 47 8 0. 37 3 0. 36 1 � 0. 00 2 0. 72 4 2. 23 8 9. 39 6 0. 05 1 S S R 7 � 1. 83 9 0. 20 8 L og T ox ap he ne D eh ch o 7 � 0. 19 2 0. 32 9 � 0. 19 0 � 0. 53 3 0. 15 3 � 1. 21 5 11 .1 97 0. 24 9 S S R 7 � 0. 00 2 0. 25 0 L og ΣC H L D eh ch o 7 � 1. 05 2 0. 29 6 0. 03 3 � 0. 28 1 0. 34 6 0. 23 11 0. 82 3 S S R 7 � 1. 08 5 0. 23 7 L og ΣH C H D eh ch o 7 � 0. 85 9 0. 14 3 � 0. 12 3 � 0. 27 7 0. 03 � 1. 76 11 .5 53 0. 10 5 S S R 7 � 0. 73 6 0. 11 8 L og ΣC B Z D eh ch o 7 � 0. 47 9 0. 44 3 � 0. 35 1 � 0. 76 7 0. 06 6 � 1. 95 4 7. 72 2 0. 08 8 S S R 7 � 0. 12 8 0. 17 L og ΣM on o_ D i- C B D eh ch o 7 � 1. 50 3 1. 08 6 � 1. 08 1 � 2. 11 8 � 0. 04 � 2. 37 5 8. 61 6 0. 04 3 S S R 7 � 0. 42 2 0. 52 0 L og ΣT ri -C B D eh ch o 7 � 1. 84 4 0. 83 0 � 0. 60 8 � 1. 40 2 0. 18 7 � 1. 73 9 8. 73 5 0. 11 7 S S R 7 � 1. 23 6 0. 40 8 L og ΣT et ra -C B D eh ch o 7 � 1. 30 1 0. 69 0 0. 00 9 � 0. 63 9 0. 65 8 0. 03 3 7. 71 2 0. 97 5 S S R 7 � 1. 31 0. 26 4 L og Σh ex a- C B D eh ch o 7 � 1. 34 9 0. 62 1 0. 34 2 � 0. 25 1 0. 93 6 1. 31 4 8. 63 5 0. 22 3 S S R 7 � 1. 69 2 0. 29 9 30 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 10 B . C om pa ri so n of m ea n co nc en tr at io ns (l og ng /g w et w t) of m aj or in di vi du al ha lo ge na te d or ga ni c co nt am in an ts in m oo se liv er fr om th e D eh ch o an d S ou th S la ve (S S R ) re gi on s us in g th e S tu de nt s t- te st as su m in g se pa ra te va ri an ce ; si gn if ic an t va lu es ar e bo ld ed (P < 0. 05 ). V ar ia bl e R eg io n n M ea n S ta nd D ev M ea n D if fe re nc e L ow er 95 % C I U pp er 95 % C I t df p- V al ue L og H C B D D eh ch o 7 � 1. 98 2 0. 13 0. 40 6 0. 25 4 0. 55 8 5. 81 12 0. 00 1 S S R 7 � 2. 38 8 0. 13 1 L og 12 45 -T eC B z JM R 7 � 1. 80 4 1. 11 9 � 0. 93 2 � 1. 96 8 0. 10 3 � 2. 18 4 6. 22 4 0. 07 S S R 7 � 0. 87 2 0. 15 3 L og 12 34 -T eC B z JM R 7 � 1. 78 5 1. 14 4 � 0. 93 2 � 1. 99 0. 12 6 � 2. 13 9 6. 18 1 0. 07 5 S S R 7 � 0. 85 3 0. 14 L og P eC B z D eh ch o 7 � 1. 43 6 0. 72 5 � 0. 59 9 � 1. 27 1 0. 07 3 � 2. 14 7 0. 07 2 S S R 7 � 0. 83 7 0. 16 L og H C B D eh ch o 7 � 0. 77 9 0. 13 5 � 0. 22 9 � 0. 50 5 0. 04 6 � 1. 90 8 0. 09 2 S S R 7 � 0. 54 9 0. 29 L og P C A D eh ch o 7 � 2. 03 0. 67 � 0. 39 7 � 1. 05 1 0. 25 6 � 1. 36 10 0. 20 4 S S R 7 � 1. 63 3 0. 38 1 L og α- H C H D eh ch o 7 � 1. 62 3 0. 51 3 � 0. 34 1 � 0. 81 8 0. 13 7 � 1. 68 3 7. 07 0. 11 8 S S R 7 � 1. 28 2 0. 15 4 L og β- H C H D eh ch o 7 � 1. 10 9 0. 08 1 0. 04 4 � 0. 10 8 0. 19 7 0. 65 7 8. 92 0. 52 3 S S R 7 � 1. 15 3 0. 15 9 L og γ- H C H D eh ch o 7 � 1. 68 4 0. 31 3 � 0. 44 � 0. 74 1 � 0. 13 8 � 3. 30 7 8. 93 0. 00 6 S S R 7 � 1. 24 4 0. 16 0. 11 8 L og he pt ac hl or -e po xi de D eh ch o 7 � 1. 48 0 0. 38 7 � 0. 00 2 � 0. 38 5 0. 38 � 0. 01 4 9. 92 3 0. 98 9 S S R 7 � 1. 47 7 0. 23 6 L og ox yc hl or da ne D eh ch o 7 � 1. 7 0. 50 8 � 0. 04 0 � 0. 57 7 0. 49 7 � 0. 16 11 0. 87 3 S S R 7 � 1. 66 0. 40 1 L og ci s- ch lo rd an e D eh ch o 7 � 1. 96 5 0. 55 2 � 0. 28 0 � 0. 80 3 0. 24 3 � 1. 23 8 0. 25 4 S S R 7 � 1. 68 6 0. 24 1 Ta bl e S 10 B co nt in ue d . . . . ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 31 Ta bl e S 10 B co nt in ue d V ar ia bl e R eg io n n M ea n S ta nd D ev M ea n D if fe re nc e L ow er 95 % C I U pp er 95 % C I t df p- V al ue L og α- en do su lf an D eh ch o 7 � 2. 09 1 0. 42 4 0. 35 3 � 0. 14 7 0. 85 4 1. 54 12 0. 15 0 S S R 7 � 2. 44 5 0. 43 5 L og en do su lf an su lf at e D eh ch o 7 � 1. 82 5 0. 47 9 0. 52 1 0. 06 2 0. 98 0 2. 58 9 0. 03 0 S S R 7 � 2. 34 6 0. 23 6 L og m ir ex D eh ch o 7 � 2. 60 4 0. 43 7 � 0. 03 1 � 0. 53 6 0. 47 4 � 0. 13 5 12 0. 89 5 S S R 7 � 2. 57 3 0. 43 0 L og B D E 47 D eh ch o 7 � 1. 43 7 0. 85 1 � 0. 09 7 � 0. 89 0 0. 69 7 � 0. 29 7 0. 78 3 S S R 7 � 1. 34 1 0. 27 7 L og P F N A D eh ch o 7 � 0. 61 5 0. 14 2 0. 15 3 � 0. 05 7 0. 36 3 1. 61 11 0. 13 7 S S R 7 � 0. 76 8 0. 20 7 L og P F D A D eh ch o 7 � 0. 69 0. 26 0. 13 8 � 0. 12 6 0. 40 2 1. 15 11 0. 27 4 S S R 7 � 0. 82 7 0. 17 9 L og P F U N A D eh ch o 7 � 0. 81 7 0. 2 0. 22 6 0. 02 8 0. 42 5 2. 54 10 0. 02 9 S S R 7 � 1. 04 4 0. 12 6 L og P F O S D eh ch o 7 � 0. 42 3 0. 24 3 0. 18 9 � 0. 04 3 0. 42 1 1. 86 9 0. 09 8 S S R 7 � 0. 61 2 0. 11 7 L og P F B S D eh ch o 7 � 1. 28 0. 15 4 0. 46 7 � 0. 07 8 1. 01 2 2. 03 5 6. 82 7 0. 08 2 S S R 7 � 1. 74 7 0. 58 7 L og P F H xS D eh ch o 7 � 1. 24 6 0. 22 6 0. 94 0. 48 1. 4 4. 66 3 8. 50 4 0. 00 1 S S R 7 � 2. 18 6 0. 48 3 L og P C B 15 D eh ch o 7 � 1. 96 9 1. 29 � 0. 64 5 � 1. 90 3 0. 61 4 � 1. 15 10 0. 27 8 S S R 7 � 1. 32 5 0. 73 3 L og P C B 17 D eh ch o 7 � 1. 60 7 0. 45 4 0. 19 9 � 0. 25 9 0. 65 8 0. 96 11 0. 35 8 S S R 7 � 1. 80 6 0. 30 7 Ta bl e S 10 B co nt in ue d . . . . 32 POPS IN MOOSE LIVERS – LARTER ET AL. ALCES VOL. 53, 2017 Ta bl e S 10 B co nt in ue d V ar ia bl e R eg io n n M ea n S ta nd D ev M ea n D if fe re nc e L ow er 95 % C I U pp er 95 % C I t df p- V al ue L og C B 20 _3 3_ 21 D eh ch o 7 � 1. 86 9 0. 61 6 0. 06 4 � 0. 68 5 0. 81 2 0. 19 12 0. 85 5 S S R 7 � 1. 93 2 0. 66 8 L og P C B 56 _6 0 D eh ch o 7 � 2. 10 9 1. 01 � 0. 67 5 � 1. 61 9 0. 26 8 � 1. 68 7 0. 13 5 S S R 7 � 1. 43 3 0. 34 2 L og P C B 15 3 D eh ch o 7 � 2. 60 7 0. 36 8 � 0. 47 8 � 0. 96 2 0. 00 6 � 2. 16 11 0. 05 3 S S R 7 � 2. 12 9 0. 45 5 ALCES VOL. 53, 2017 LARTER ET AL. – POPS IN MOOSE LIVERS 33 PERSISTENT ORGANIC POLLUTANTS IN THE LIVERS OF MOOSE HARVESTED IN THE SOUTHERN NORTHWEST TERRITORIES, CANADA ANALYTICAL METHODS PCBs, OCOs, and BFRs PFASs QUALITY ASSURANCE title_bkm_6