AMERICAN INTERNATIONAL JOURNAL OF AGRICULTURAL STUDIES 6(1) (2022), 12-19 12 AGRICULTURAL STUDIES AIJAS VOL 6 NO 1 (2022) P-ISSN 2641-4155 E-ISSN 2641-418X Available online at www.acseusa.org Journal homepage: https://www.acseusa.org/journal/index.php/aijas Published by American Center of Science and Education, USA PERFORMANCE OF TELFAIRIA OCCIDENTALIS LEAF GROWN IN UREA HYDROPONIC SOLUTION Love Akaja Akonye (a) Kalu Okonwu (b)1 Josephine Udunma Agogbua (c ) (a) Professor, Department of Plant Science and Biotechnology, University of Port Harcourt, Nigeria; E-mail: love.akonye@uniport.edu.ng (b) Department of Plant Science and Biotechnology, University of Port Harcourt, Nigeria; E-mail: kalu.okonwu@uniport.edu.ng (c) Department of Plant Science and Biotechnology, University of Port Harcourt, Nigeria; E-mail: josephine.agogbua@uniport.edu.ng A R T I C L E I N F O Article History: Received: 26th July 2022 Accepted: 25th August 2022 Online Publication: 3rd September 2022 Keywords: Hydroponic, Grown, Performance Telfairia Occidentalis JEL Classification Codes: O13 A B S T R A C T The study evaluated the performances of Telfairia occidentalis Hooker fil. under varying growth media subject to the amount of Urea granules (25 g, 50 g, 75 g, 100 g, 125 g and 150 g) dissolved in water containing micronutrients. The growth media were M25U, M50U, M75U, M100U, M125U, M150U, and Control. Two-week old seedlings of T. occidentalis raised using River-sand were transferred into the non-circulatory growth media. The growth indices (vine main length (VML), number of leaves (NL), stem girth (SG), petiole length (PL), internode (LI), leaf area (LA), and total leaf area (TLA)) of T. occidentalis were measured weekly. The root length (RL), root fresh weight (RFW), root dry weight (RDW) and pigment components were determined 5 weeks after planting following standard procedures. Across the growth media studied, the Control medium had the highest VML, NL, LA, TLA, PL and pigment composition of T. occidentalis. However, among the Urea growth media, M25U medium produced relatively the highest VML, NL, TLA, total chlorophyll, and RFW of T. occidentalis while M50U medium had the highest LA, RL and RDW. Also, T. occidentalis leaves grown in the Control medium had the highest chlorophyll content (33.22 mg/g), followed by M25U medium (22.88 mg/g) and was significantly different from the other growth media.M100U medium effectively enhanced carotenoids content (6.49 mg/g) of T. occidentalis compared to others. The study showed that the mineral composition of the growth media enhanced the performance of T. occidentalis. Hence, M25U growth media are recommended for growing T. occidentalis. © 2022 by the authors. Licensee ACSE, USA. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). INTRODUCTION Plants do not necessarily need soil in order to grow and survive. According to White and Brown (2010), plants obtain their inorganic elements from the soil solution. Soil serves as a medium to support plant and to retain nutrients for plants’ utilization. Hence, any medium that is stable enough to support plant and retain nutrients can do the same job as soil without being restricted to the ground. Hydroponic is the cultivation of plants, edible and ornamental, in water containing dissolved nutrients. Growing fresh produce in soilless systems could be a possible solution to food insecurity issues regardless of soil quality, climate or space (Resh & Howard, 2012). According to Santos and Ocampo (2005), hydroponics provides an instant and also long-term remedy to the difficult of inability of a household to produce its own vegetables under urban settings. A hydroponic system allows for uninterrupted vegetable production no matter the period (Santos & Ocampo, 2009). It is one of the technologies used in places not appropriate for traditional farming systems (Pelesco & Bentor-Jr., 2013). This helps to mitigate the difficulty of climate change, assist in production system management for efficient exploitation of natural resources and alleviation of malnutrition (Butler & Oebker, 2006). The use of controlled environments can overcome cultivation difficulties and could be a way to manipulate phenotypic variation in bioactive compounds (Murali-Mugundhan et al., 2011). It has been claimed that the key to successful hydroponics culture is the nutrient solution (Hedio, 2000). To achieve optimized growing systems, different types of crops, nutrient solutions, lighting and other factors are important when determining the crops that will be successful in hydroponic systems from environmental, economic and nutritional perspectives (Treftz & Omaye, 2015). Previous literature has focused on various hypotheses of growing cucumbers, tomatoes, carrots, peppers and strawberries hydroponically (Arias et al., 2000; Parađiković et al., 2011; Coolong, 2012). 1Corresponding author: ORCID ID: 0000-0003-4140-5250 © 2022 by the authors. Hosting by ACSE. Peer review under responsibility of American Center of Science and Education, USA. https://doi.org/10.46545/aijas.v6i1.256 To cite this article: Akonye, L. A. ., Okonwu, K. ., & Agogbua, J. U. (2022). PERFORMANCE OF TELFAIRIA OCCIDENTALIS LEAF GROWN IN UREA HYDROPONIC SOLUTION. American International Journal of Agricultural Studies, 6(1), 12–19. https://doi.org/10.46545/aijas.v6i1.256 https://doi.org/10.46545/aijas.v6i1.256 http://creativecommons.org/licenses/by/4.0/) http://creativecommons.org/licenses/by/4.0/) https://orcid.org/0000-0003-3903-2408 https://orcid.org/0000-0003-4140-5250 https://orcid.org/0000-0001-6317-1227 Akonye et al., American International Journal of Agricultural Studies 6(1) (2022), 12-19 13 According to Hickman (2011), the main greenhouse crops (including soil and soilless production) are tomato, cucumber, sweet pepper, herbs, eggplant and strawberry, in that sequence. Resh (1995), earlier reported that the major vegetables grown in soilless cultivation are tomato, cucumber, sweet pepper and L. sativa in that order. But in Latin America, the main vegetables include; L. sativa, arugula, tomato, sweet pepper, cucumber and strawberry (Rodriguez-Delfin, 2012) in that same order. With the hydroponic technology being used more frequently, it has become imperative to assess which crops are suitable to be grown in hydroponic systems. The production of vegetable crops and ornamentals using different soilless culture techniques has been practiced and commercialized (Kratky et al., 1988; Pelesco & Bentor-Jr., 2013). However, hydroponic as a way of growing plants is rarely practiced in Nigeria even though the technology had long existed in the industrialized nations. Hydroponics as a means of growing plants should be embraced in unindustrialized nations like Nigeria. The ability to formulate nutrients locally to grow plants should be of priority to researchers. The cost of producing plants will reduce tremendously if required materials are sourced locally and are affordable to farmers. However, this study is aimed at using urea fertilizer in the formulation of locally hydroponic nutrient solutions for growing T. occidentalis and evaluate how the solutions affects its performance. MATERIALS AND METHODS Source of Materials The seeds of fluted pumpkin used were sourced from Choba Market and the River-sand was obtained from Choba-River Port Harcourt (4o54′0ʺN 6o54′0ʺE) while the urea fertilizer used was produced by Unique Fertilizer Company Nigeria and obtained from Agricultural Development Programme, Rumuodumaya Port Harcourt. Formulation of the Nutrient Solution The method of Kratky (2002) was used with modification in nutrient formulation and container used. Urea granular fertilizers were weighed (25 g, 50 g, 75 g, 100 g, 125 g and 150 g, respectively) and transferred into black plastic bowls with the dimensions: 29 cm width, 41 cm length, and 23 cm depth. The same was dissolved with 20 litres of tap water in the plastic bowls leaving a space for aeration with the addition of 20 ml micronutrients stock solution (0.6 g H3BO3; 0.4 g MnCl2.4H2O; 0.05 g ZnSO4; 0.5 g CuSO4.5H2O; 0.02 g Na2MoO4.2H2O) and Epsom salt (9.8 g MgSO4). The Control medium (water) was setup without the addition of Urea, micronutrients and Epsom salt. These formulations were replicated four times. The growth media were designated as: M25U, M50U, M75U, M100U, M125U, M150U, and Control (0 g Urea) depending on the amount urea dissolved in water. Study Site and Weather Condition The study was conducted in a screen house inside the University of Port Harcourt (Lat. N4o54'15", Long. E6o54'35"). The site was free from direct rainfall and was open to sunlight anytime of the day. The screen house had a transparent cover that permits light penetration and hinders direct rainfall. During the period of experiment, the weather condition of the University was relatively wet with daytime temperature that ranges from 24oC in early morning to 32oC in the middle part of the day. Planting of T. occidentalis The seeds of T. occidentalis were planted in nursery bags containing River-sand as a medium for germination to take place. After germination, the two weeks old seedlings (17 - 20 cm) from the River-sand were transferred into the non-circulating hydroponic systems containing different formulations of nutrient solution. The components of the nutrient solutions were sourced locally. Growth Indices Measurement The vine length, petiole length and internode were measured using meter rule while the number of leaves was by direct count. The leaf area was determined following the method of Akoroda (1993). The vine girth was determined with the aid of electronic digital caliper (Carbon Fiber Composites Digital Caliper). Pigment Content The chlorophyll content was obtained according to the method of Poora (2002) while the carotenoid content was determined following the method of Sumanta et al. (2014). Statistical Analysis The data obtained for the morphological characters and pigment contents of fluted pumpkin were subjected to statistical analysis. RESULTS Vine Main Length of T. occidentalis Grown in Different Growth Media The growth performance of T. occidentalis in varying Urea solutions with respect to vine main length (VML) are presented in Table 1. The rate at which the VML value for Control treatment increased (28.63 – 57.05 cm) after week 4 was faster when compared to other growth media. This was followed by M25U growth medium (27.98 – 37.23 cm). The percentage increase in VML values were 99.27%, 33.06%, 33.87%, 2.66%, 0.55%, 4.12% and 0.23% for Control, M25U, M50U, M75U, M100U, M125U and M150U growth media, respectively. The result of increasing the Urea concentration in the medium did not produce an increased VML. At 5 Weeks after planting, the lowest VML (21.95 ± 4.944 cm) was obtained at M150U medium and the VML value for Control medium was significantly different from other media. Akonye et al., American International Journal of Agricultural Studies 6(1) (2022), 12-19 14 Table 1. Vine main length (cm) of T. occidentalis in different Urea growth media Treatment Duration Mean Percentage Increase (4 – 5 WAP) 3 WAP 4 WAP 5 WAP Control 23.23 ± 5.752a 28.63 ± 10.036a 57.05 ± 18.018a 36.300a 99.27 M25U 22.55 ± 3.594a 27.98 ± 7.083a 37.23 ± 12.831b 29.250ab 33.06 M50U 17.10 ± 6.434a 18.60 ± 4.614a 24.90 ± 7.099b 20.200c 33.87 M75U 23.15 ± 5.501a 30.13 ± 13.818a 30.93 ± 14.695b 28.067bc 2.66 M100U 25.58 ± 3.007a 27.50 ± 2.783a 27.65 ± 3.226b 26.908bc 0.55 M125U 23.93 ± 6.777a 26.73 ± 9.554a 27.83 ± 10.169b 26.158bc 4.12 M150U 21.23 ± 4.307a 21.90 ± 4.917a 21.95 ± 4.944b 21.692bc 0.23 Mean 22.393 ± 5.257b 25.921 ± 8.3164b 32.504 ± 14.9683a LSD(P=0.05) 7.6847 12.257 16.617 8.0275 Mean ± Standard deviation; Means with the same letter in a column are not significantly different; WAP = weeks after planting. Stem Girth of T. occidentalis Grown in Different Growth Media The stem girth of T. occidentalis grown in different concentration of Urea solutions is shown in Table 2. There was no exponential growth in the stem girth in all the growth media. The stem girth from week 3 – 5 for the growth media ranged thus: Control (4.55 – 5.35 mm), M25U (4.68 – 4.88 mm), M50U (4.73 – 5.33 mm), M75U (3.83 – 4.60 mm), M100U (3.93 – 5.83 mm), M125U (3.93 – 4.43 mm) and M150U (2.73 – 4.98 mm). Amongst growth media, the percentage increase in stem girth was 17.58%, 4.27%, 12.68%, 20.10%, 48.35%, 12.72% and 82.42% for Control, M25U, M50U, M75U, M100U, M125U and M150U growth media, respectively. M150U medium had the highest percentage increase of stem girth from week 3 – 5. However, at week 5, the highest value for stem girth (5.83 ± 0.670 mm) was recorded at M100U growth medium while the lowest (4.43 ± 0.670 mm) at M125U growth medium. These values were statistically different at P = 0.05. Considering the mean growth rate of the stem girth from 3 – 5 WAP, M50U growth medium had the stem girth of T. occidentalis, followed by Control, M25U, M100U, M75U, M125U and M150U, in that order. The mean stem girth value for M50U was significantly different from M75U, M125U and M150U, respectively. Table 2. Stem girth (mm) of T. occidentalis in different Urea growth media Treatment Duration Mean 3 WAP 4 WAP 5 WAP Control 4.55 ± 0.635a 4.65 ± 0.785a 5.35 ± 0.370ab 4.8500ab M25U 4.68 ± 0.754a 4.70 ± 1.344a 4.88 ± 1.330ab 4.7500ab M50U 4.73 ± 1.609a 5.28 ± 1.287a 5.33 ± 1.253ab 5.1083a M75U 3.83 ± 0.888ab 3.88 ± 0.939ab 4.60 ± 0.821ab 4.1000bc M100U 3.93 ± 0.613ab 3.93 ± 0.613ab 5.83 ± 0.670a 4.5583ab M125U 3.93 ± 1.307ab 3.93 ± 1.307ab 4.43 ± 0.670b 4.0917bc M150U 2.73 ± 0.858b 2.73 ± 0.858b 4.98 ± 0.411ab 3.4750c Mean 4.0543 ± 1.184b 4.1536 ± 1.2088b 5.0534 ± 0.9094a LSD(P=0.05) 1.4605 1.5505 1.3014 0.8684 Mean ± Standard deviation; Means with the same letter in a column are not significantly different; WAP = weeks after planting. Number of Leaves of T. occidentalis Grown in Different Growth Media The number of leaves grown in different concentrations of Urea growth media are presented in Table 3. Increased quantity of Urea did not record a definite pattern in terms of size (either increase or decrease) in the number of leaves of T. occidentalis. The percentage increase in the number of leaves from weeks 3 – 5 was: 34.38%, 30.00%, 13.04%, 24.14%, 12.12%, 10.71% and 3.13%, respectively for the Control, M25U, M50U, M75U, M100U, M125U and M150U media. Control medium had the highest percentage increase for number of leaves of T. occidentalis from week 3 – 5, followed by M25U growth medium. Hence, the highest mean value for number of leaves (10.75 ± 0.957) was recorded at the Control medium while the lowest (6.50 ± 1.915) was recorded at M50U medium at week 5. Table 3. Number of leaves of T. occidentalis in different Urea growth media Treatment Duration Mean 3 WAP 4 WAP 5 WAP Control 8.00 ± 0.816a 8.75 ± 0.500a 10.75 ± 0.957a 9.1667a M25U 7.50 ± 0.577ab 8.75 ± 1.708a 9.75 ± 2.062ab 8.6667a M50U 5.75 ± 2.754b 6.00 ± 2.449b 6.50 ± 1.915c 6.0833c M75U 7.25 ± 1.258ab 8.50 ± 1.000ab 9.00 ± 0.816ab 8.2500ab M100U 8.25 ± 0.957a 9.25 ± 0.957a 9.25 ± 0.957ab 8.9167a M125U 7.00 ± 1.414ab 7.75 ± 1.893ab 7.75 ± 1.893bc 7.5000b M150U 8.00 ± 1.633a 8.00 ± 1.633ab 8.25 ± 1.258bc 8.0833ab Mean 7.3929 ± 1.5477b 8.1429 ± 1.7152ab 8.7500 ± 1.8584a LSD(P=0.05) 2.2058 2.3140 2.1941 1.1475 Mean ± Standard deviation; Means with the same letter in a column are not significantly different; WAP = weeks after planting. Akonye et al., American International Journal of Agricultural Studies 6(1) (2022), 12-19 15 Leaf Petiole Length of T. occidentalis Grown in Different Growth Media The leaf petiole length (LPL) of T. occidentalis grown in varying concentrations of Urea growth media varied across treatments (Table 4). The rate at which the petiole lengths increased were not significant at p≤0.05 across and within growth media from weeks 3 – 5. The ranges within growth media were: Control (3.90 – 4.90 cm), M25U (4.10 – 4.38 cm), M50U (4.03 – 4.95 cm), M75U (4.20 – 4.98 cm), M100U (4.30 – 4.85 cm), M125U (4.33 – 4.45 cm) and M150U (4.33 – 5.25 cm). Amongst growth media, the percentage increase in the petiole lengths was 25.64%, 6.83%, 22.83%, 18.57%, 12.79%, 2.77% and 21.25% for Control, M25U, M50U, M75U, M100U, M125U and M150U treatments, respectively. Control medium had the highest percentage increase of LPL from weeks 3 – 5. However, at week 5, the highest mean value for LPL (5.25 ± 1.611 cm) was recorded at M150U medium while the lowest (4.38 ± 0.479 cm) at M25U medium. There was no significant difference among growth media. Table 4. Petiole length (cm) of T. occidentalis leaves in different Urea growth medium Treatment Duration Mean 3 WAP 4 WAP 5 WAP Control 3.90 ± 1.052a 4.20 ± 1.071a 4.90 ± 0.383a 4.3333a M25U 4.10 ± 0.577a 4.20 ± 0.589a 4.38 ± 0.479a 4.2250a M50U 4.03 ± 1.343a 4.88 ± 1.758a 4.95 ± 1.771a 4.6167a M75U 4.20 ± 0.712a 4.25 ± 0.742a 4.98 ± 1.150a 4.4750a M100U 4.30 ± 0.503a 4.33 ± 0.472a 4.85 ± 0.719a 4.4917a M125U 4.33 ± 0.914a 4.45 ± 1.248a 4.45 ± 1.248a 4.4083a M150U 4.33 ± 1.584a 4.33 ± 1.584a 5.25 ± 1.611a 4.6333a Mean 4.3107 ± 0.8842a 4.3750 ± 1.0473a 4.6786 ± 1.1622a LSD(P=0.05) 1.4047 1.7059 1.7980 1.569 Mean ± Standard deviation; Means with the same letter in a column are not significantly different; WAP = weeks after planting. Leaf Internodes of T. occidentalis Grown in Different Growth Media The growth performance of T. occidentalis in varying Urea solutions with respect to leaf internodes are presented in Table 5. There was increase in leaf internodes from weeks 3 – 5 across growth media. The leaf internodes value was higher in the Control medium compared to other growth media for weeks 3 – 5 after seedlings were transferred into the growth media. The percentage increase in leaf internodes values was 4.09%, 16.23%, 11.54%, 23.77%, 13.65%, 4.48% and 6.00% for Control, M25U, M50U, M75U, M100U, M125U and M150U growth media, respectively. The effect of increasing the Urea concentration in the medium did not record a definite pattern in terms of leaf internodes. The least value of leaf internodes (2.90 ± 1.023 cm) was recorded at M50U. Table 5. Internode (cm) of T. occidentalis leaves in different Urea growth medium Treatment Duration Mean 3 WAP 4 WAP 5 WAP Control 4.40 ± 1.444a 4.50 ± 2.380a 4.58 ± 0.419a 4.4917a M25U 3.08 ± 0.435b 3.15 ± 0.465ab 3.58 ± 1.014a 3.2667b M50U 2.60 ± 0.542b 2.80 ± 1.023b 2.90 ± 1.023a 2.7667b M75U 3.23 ± 1.452b 3.30 ± 1.402ab 4.03 ± 1.297a 3.5167b M100U 2.93 ± 0.737b 2.95 ± 0.695b 3.33 ± 0.427a 3.0667b M125U 3.35 ± 0.473b 3.50 ± 0.959ab 3.50 ± 0.959a 3.4500b M150U 2.83 ± 0.330b 2.83 ± 0.330b 3.00 ± 1.042a 2.8833b Mean 3.203 ± 0.8805a 3.290 ± 1.0175a 3.560 ± 1.2542a LSD(P=0.05) 1.0608 1.4525 1.8941 0.7858 Mean ± Standard deviation; Means with the same letter in a column are not significantly different; WAP = weeks after planting. Leaf Area of T. occidentalis Grown in Different Growth Media Table 6 shows the leaf area of T. occidentalis grown in different Urea growth media. There was an increase in the leaf area from weeks 3 – 5 and the growth performance varied across the growth media. The leaf area ranged from 110.28 – 138.00 cm2, 105.39 – 117.57 cm2, 135.59 – 147.29 cm2, 93.73 – 98.10 cm2, 86.93 – 89.93 cm2, 95.54 – 111.85 cm2, and 94.56 – 100.26 cm2 for Control, M25U, M50U, M75U, M100U, M125U and M150U growth media, respectively. The M50U medium had the highest leaf area value (147.29 ± 59.395 cm2) at week 5, followed by Control medium (138.00 ± 17.617 cm2) while the lowest value (89.93 ± 29.796 cm2) was recorded at M100U medium. These highest and lowest points had the percentage leaf area increase from weeks 3 – 5 as follows: 25.14%, 8.63% and 3.45% for Control, M50U and M100U media, respectively. However, there was no significant difference (P = 0.05) amongst growth media from weeks 4 – 5. Table 6. Leaf area (cm2) of T. occidentalis in different Urea growth media Treatment Duration Mean 3 WAP 4 WAP 5 WAP Control 110.28 ± 5.576ab 124.39 ± 14.924a 138.00 ± 17.617a 124.22ab M25U 105.39 ± 44.783ab 116.28 ± 32.724a 117.57 ± 33.129a 113.08bc M50U 135.59 ± 48.126a 143.36 ± 59.317a 147.29 ± 59.395a 142.08a M75U 93.73 ± 8.203ab 95.88 ± 19.324a 98.098 ± 17.588a 95.90c Akonye et al., American International Journal of Agricultural Studies 6(1) (2022), 12-19 16 M100U 86.93 ± 32.688b 89.93 ± 29.796a 89.93 ± 29.796a 92.26c M125U 95.54 ± 27.507ab 111.85 ± 37.758a 111.85 ± 37.758a 106.42bc M150U 94.558 ± 6.870ab 100.26 ± 30.741a 100.26 ± 30.741a 101.69bc Mean 103.145 ± 29.792a 111.706 ± 36.223a 114.711 ± 36.367a LSD(P=0.05) 43.451 52.077 51.795 26.303 Mean ± Standard deviation; Means with the same letter in a column are not significantly different; WAP = weeks after planting. Total Leaf Area of T. occidentalis Grown in Different Growth Media The total leaf area of T. occidentalis grown in different Urea growth media for 5 weeks is shown in Table 7. There was increase in the total leaf area from weeks 3 – 5 across growth media. The total leaf area value was higher in the Control medium compared to other growth media for weeks 3 – 5 and it was significantly different (P = 0.05) from other growth media except M25U medium. There was rapid increase in the total leaf area value for all growth media. The percentage increase from weeks 3 – 5 for total leaf area values was 68.58%, 41.98%, 26.51%, 31.68%, 5.74%, 31.52% and 9.26% for Control, M25U, M50U, M75U, M100U, M125U and M150U growth media, respectively. At week 5, the Control medium (1492.04 ± 298.148 cm2) had the highest total leaf area value, followed by M25U medium (1119.77 ± 286.000 cm2). The lowest mean value for total leaf area (821.40 ± 253.916 cm2) was recorded at M100U medium. Table 7. Total leaf area (cm2) of T. occidentalis in different Urea growth media Treatment Duration Mean 3 WAP 4 WAP 5 WAP Control 885.08 ± 126.747a 1084.72 ± 102.990a 1492.04 ± 298.148a 1153.9a M25U 788.69 ± 356.772a 1000.77 ± 273.130a 1119.77 ± 286.000ab 969.7ab M50U 690.22 ± 134.854a 751.36 ± 90.560a 873.17 ± 131.333b 771.6b M75U 677.54 ± 117.140a 823.05 ± 236.101a 892.19 ± 225.461b 797.6b M100U 776.81 ± 177.727a 821.40 ± 253.916a 821.40 ± 253.916b 806.5b M125U 692.83 ± 305.588a 911.22 ± 464.163a 911.22 ± 464.163b 838.4b M150U 785.33 ± 222.153a 785.33 ± 222.153a 858.06 ± 96.061b 809.6b Mean 756.636 ± 209.2924b 882.55 ± 257.7472ab 995.4064 ± 331.0746a LSD(P=0.05) 328.62 384.44 403.83 215.47 Mean ± Standard deviation; Means with the same letter in a column are not significantly different; WAP = weeks after planting. Pigments Composition of T. occidentalis Leaves Grown in Different Urea Growth Media The pigments composition of T. occidentalis leaves grown in different Urea growth media at 5 WAP are as shown in Table 8. The chlorophyll and carotenoid contents of the leaves varied and were significantly different (P ≤ 0.05) amongst growth media. Chlorophyll a content of the leaves was higher than chlorophyll b in all the growth media. Telfairia occidentalis leaves grown in the Control medium had the highest chlorophyll content and was significantly different from the other growth media. The lowest chlorophyll content of the leaves was recorded at M150U medium. The chlorophyll a, chlorophyll b and total chlorophyll contents ranged from 2.50 – 17.31 mg/g, 1.13 – 15.90 mg/g and 3.63 – 33.22 mg/g, respectively. However, the result obtained for carotenoid of the leaves was different. The highest carotenoid content (6.49 mg/g) of the leaves was recorded at M100U medium which was significantly different from other growth media while the lowest carotenoid content (0.45 mg/g) was recorded at M25U medium. The carotenoid contents were lower compared to the chlorophyll a content. Table 8. Pigments composition (mg/g) of T. occidentalis leaves in different Urea growth media at 5 WAP Treatment Chlorophyll a Chlorophyll b Total Chlorophyll Carotenoid Control 17.31 ± 0.191a 15.90 ± 0.942a 33.22 ± 1.131a 2.82 ± 0.320dc M25U 14.81 ± 1.142b 8.07 ± 0.243b 22.88 ± 1.357b 0.45 ± 0.125e M50U 13.45 ± 1.218b 8.25 ± 1.534b 21.70 ± 2.700b 4.53 ± 0.375b M75U 4.39 ± 0.292d 2.35 ± 0.389c 6.74 ± 0.681c 3.63 ± 0.286bc M100U 10.78 ± 3.241c 7.94 ± 2.831b 18.72 ± 5.969b 6.49 ± 2.215a M125U 3.94 ± 1.578d 2.451 ± 1.021c 6.393 ± 2.387c 3.78 ± 0.878bc M150U 2.50 ± 0.295d 1.13 ± 0.254c 3.63 ± 0.533c 1.713 ± 0.460de LSD(P=0.05) 2.4185 2.1463 4.3792 1.5723 Mean ± Standard deviation; Means with the same letter in a column are not significantly different; WAP = weeks after planting. Root Length, Root Fresh Weight and Root Dry Weight of T. occidentalis Grown in Different Growth Media at 5 WAP The root length also varied in different Urea growth media (Figure 1). The root lengths ranged from 15.08 – 24.20 cm and the values recorded fluctuate across growth media. Telfairia occidentalis grown in M50U medium had the highest root length (24.20 cm) compared to other media, which was not significantly different at p = 0.05 while the lowest was recorded at M150U growth media. The root fresh weight decreased as the quantity of Urea increased in the growth media (Figure 2). The root fresh weight values of T. occidentalis ranged from 2.24 – 8.77 g. The root fresh weight values recorded for Urea growth medium were statistically different (P = 0.05) with the Control medium. Among the growth media, the Control medium had the highest (8.77 g) root fresh weight while the lowest value (2.24 g) was recorded at M150U medium. Akonye et al., American International Journal of Agricultural Studies 6(1) (2022), 12-19 17 The root dry weight values of T. occidentalis varied across the Urea growth media and ranged from 0.17 – 0.71 g (Figure 3). Among the growth media, the Control medium had the highest root dry weight (0.71 g) while the lowest value (0.17 g) was recorded at M125U medium. There was significant difference in the value recorded for Control medium and other growth media. DISCUSSIONS The performance of T. occiddentalis varied across Urea growth media (M25U, M50U, M75U, M100U, M125U, M150U).M25U medium effectively enhanced VML, NL, LA, TLA, carotenoids content, RL, RFW and RDW. This suggest that lower quantity of macronutrients in hydroponic solution will improve the performance of T. occidentalis. The values obtained from the results for number of leaves, leaf area per leaf and total plant leaf area of T. occidentalis were in conformity with the work of Oke (2015), who reported 13.61, 118.1 cm2 and 1607.87 cm2 at 3 WAP for number of leaves, leaf area and total plant leaf area, respectively. This also agreed with works of Akanbi et al. (2000), Shiyam and Binang (2013) and Usman (2015). Ndor et al. (2012) reported the number of leaves of fluted pumpkin grown in sawdust, River sand and topsoil as 9.33, 9.00 and 8.33 respectively at two (2) weeks after sowing. However, the average values for number of leaves and leaf area per leaf of T. occidentalis at 6 WAP reported by Nwonuala and Obiefuna (2015) varied significantly from this present work. This difference probably occurred due to the mineral nutrition supplied to the plant. Although, nutrient availability is crucial in the photosynthetic capacity of a crop, it is worthy to note that the number of leaves and the surface area of leaves affect directly or indirectly the photosynthetic rate of crops. Vegetable consumers depend majorly on the leaves of fluted a a a a a a a 0 5 10 15 20 25 30 Control M25U M50U M75U M100U M125U M150U L en g th ( cm ) Growth medium Figure 1. Root length of T. occidentalis grown in different Urea growth media at 5 WAP a b b bc bc bc c 0 2 4 6 8 10 Control M25U M50U M75U M100U M125U M150U W ei g h t (g ) Growth medium Figure 2. Root fresh weight of T. occidentalis grown in different Urea growth media at 5 WAP. a b b b bc c bc 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 Control M25U M50U M75U M100U M125U M150U W ei g h t (g ) Growth medium Figure 3. Root dry weight of T. occidentalis grown in different Urea growth media at 5 WAP Akonye et al., American International Journal of Agricultural Studies 6(1) (2022), 12-19 18 pumpkin in the preparation of food and its leaves extract for medicinal purposes. This underscores the need to grow more leaves. The T. occidentalis vine main lengths obtained in different Urea treatments were lower than those reported by other researchers (Nwonuala & Obiefuna, 2015; Oke, 2015) on the same plant. They reported average vine main length of 158.30 cm and 189.61 cm at 6 WAP and 3 WAP, respectively. Similarly, the highest stem girth obtained from the treatments was 0.58 cm, which was also lower compared to the works of Ndor et al. (2012) and Oke (2015). Ndor et al. (2012) reported stem girth of T. occidentalis grown in sawdust (1.98 cm), river sand (2.10 cm) and topsoil (1.87 cm) at 2 WAP, while Oke (2015) recorded stem girth of 0.86 cm on the same plant at 3 WAP. The variation in the stem girth by several researchers could be as a result of different treatments given to T. occidentalis. This presupposes that the performance of T. occidentalis is dependent on treatment and growth medium. The leaf petiole length and internode length of T. occidentalis grown in different solutions of Urea were comparable with the values reported by Nwonuala and Obiefuna (2015), who worked on the yield and yield component of fluted pumpkin landrace. The root lengths of T. occidentalis grown in varying proportion of Urea in solution were significantly higher than the values reported by Ndor et al. (2012), who worked on growth of fluted pumpkin in different solid media. They observed and recorded the root length of T. occidentalis in different media as follows: sawdust (11.68 cm), River sand (11.12 cm) and topsoil (10.12 cm). This observation suggests that the compact nature of the growth medium used in growing T. occidentalis affects the root length. The roots of T. occidentalis grown in solution were soft and tender when compared to the texture of roots grown in other solid media (such as topsoil, River sand and sawdust). The total chlorophyll content of T. occidentalis were higher than the carotenoid content. Total chlorophyll content of T. occidentalis grown in different Urea solutions were higher compared to other vegetables (such as Chinese Cabbage, L. sativa, Broccoli, Cauliflower, Brussel’s sprout and red Cabbage) as reported by Pandey et al. (2015). They reported the total chlorophyll range of 2.23 – 16.92 mg/g. Chlorophylls are crucial components for photosynthesis (Pandey et al., 2015). According to Mustapha and Babura (2009), carotenoids comprise a large group of natural pigments widely distributed in the plant and animal kingdoms. They are yellow-orange in colour, insoluble in water but soluble in organic solvents. They are present as pigments in many vegetables and fruits and are associated with chlorophyll in higher plants, playing important role during photosynthesis by passing on the light energy they absorb to chlorophyll, they also protect the chlorophyll from excess light and oxidation. This function accounts for the low value obtained for carotenoids when compared to chlorophyll content of T. occidentalis in different growth media used. CONCLUSIONS Considering the performance of T. occidentals grown in different Urea solutions, the Control treatment had the highest vine main length, number of leaves, leaf area, total leaf area, petiole length and pigment composition of T. occidentalis compared to other treatments. However, among the Urea treatments, M25U effectively enhanced some of the growth indices such as vine main length, number of leaves, leaf area, total leaf area, total chlorophyll, and root fresh weight of T. occidentalis while M50U treatment had the highest leaf area, root length and root dry weight. The study has shown that appropriate proportion of Urea in solution can be used to grow T. occidentalis. Therefore, pre-mixed fertilizers for growing vegetables should be developed to reduce the technicality involved for the ordinary farmers, who form the larger part of the pyramid in crop production. Author Contributions: Conceptualization, L.A.A. and K.O.; Data Curation, L.A.A., K.O. and J.U.A.; Methodology, L.A.A. and K.O.; Validation, L.A.A., K.O. and J.U.A.; Visualization, L.A.A., K.O. and J.U.A..; Formal Analysis, K.O.; Investigation, J.U.A. and K.O.; Resources, L.A.A., K.O. and J.U.A.; Writing – Original Draft, K.O. and J.U.A.; Writing – Review & Editing, L.A.A., K.O. and J.U.A..; Supervision, L.A.A., K.O. and J.U.A..; Project Administration, K.O., and J.U.A.; Authors have read and agreed to the published version of the manuscript. Institutional Review Board Statement: Ethical review and approval were waived for this study, due to that the research does not deal with vulnerable groups or sensitive issues. Funding: The authors received no direct funding for this research. Informed Consent Statement: Informed consent was obtained from all subjects involved in the study. Data Availability Statement: The data presented in this study are available on request from the corresponding author. The data are not publicly available due to restrictions. Conflicts of Interest: The authors declare no conflict of interest. REFERENCES Akanbi, W. B., Baiyewu, R. A., & Tairu, F. M. (2000). Effects of maize-stover compost and spacing on growth and yield of Celosia (Celosia argentea L.). Journal of Agriculture, Forestry and Fisheries, 1, 5-9. Akoroda, M. O. (1993). Non-destructive estimation of area variation in shape of leaf lamina in the fluted pumpkin, Telfairia occidentalis. Scientia Horticulturae, 53(3), 261-267. https://doi.org/10.1016/0304-4238(93)90074-Z Arias, R., Lee, T. C., Specca, D., & Janes, H. (2000). Quality Comparison of Hydroponic Tomatoes (Lycopersicon esculentum) Ripened on and off Vine. Journal of Food Science, 65, 545-548. https://doi.org/10.1111/j.1365- 2621.2000.tb16045.x Butler, J. D., & Oebker, N. F. (2006). Hydroponics as a Hobby: Growing Plants Without Soil. Circular 844. Information Office, College of Agriculture, University of Illinois, Urbana, IL 61801. Retrieved from http://hdl.handle.net/2142/33041 Coolong, T. (2012). Hydroponic Lettuce. University of Kentucky Cooperative Extension Services, 1-4. Hedio, I. (2000). Hydroponics and /or soil less culture, Osaka Prefecture University. Pp. 6-17. Hickman, G. W. (2011). Greenhouse vegetable production statistics. Cuesta Roble Greenhouse Consultants, Mariposa, CA, USA. 73p. Akonye et al., American International Journal of Agricultural Studies 6(1) (2022), 12-19 19 Kratky, B. A. (2002). A simple hydroponic growing kit for short-term vegetables. University of Hawaii CTAHR HG-42. Retrieved from https://www.ctahr.hawaii.edu/oc/freepubs/pdf/hg-42.pdf Kratky, B. A., Bowen, J. E., & Imai, H. (1988). Observations on a non-circulating hydroponic system for tomato production. HortScience, 23: 906-907. https://doi.org/10.1016/0304-4238(88)90160-4 Murali-Mugundhan. R. M., Soundaria, M., Maheswari, V., Santhakumari, P., & Gopal, V. (2011). Hydroponics- A novel alternative for geoponic cultivation of medicinal plants and food crops. International Journal of Pharma and Bio Sciences, 2(2), 286-296. Retrieved from http://www.ijpbs.net/issue-2/38.pdf Mustapha, Y., & Babura, S. R. (2009). Determination of carbohydrate and β-carotene content of some vegetables consumed in Kano Metropolis, Nigeria. Bayero Journal of Pure and Applied Sciences, 2(1), 119-121. https://doi.org/10.4314/bajopas.v2i1.58515 Ndor, E., Dauda, N. S., & Chammang, H. B. (2012). Effect of germination media and seed size on germination and seedling vigour of fluted pumpkin (Telfairia occidentalis) Hook. F. Advances in Environmental Biology, 6(10), 2758-2761. Retrieved from http://www.internationlscholarsjournal.com/articles/effect-of-germination-media-and-seed-size-on- germination-and-seedling-vigour-of-fluted-pumpkin-telfairia-occidentalis-hoo.pdf Nwonuala, A., & Obiefuna, J. (2015). Yield and yield components of fluted pumpkin (Telfairia occidentalis Hook) landrace. International Journal of Agriculture Innovations and Research, 4(3), 421-425. Retrieved from http://www.ijair.org/administrator/components/com_jresearch/files/publications/IJAIR_1620_Final.pdf Oke, O. F. (2015). Leaf Area Development and Vine Growth of Telfairia occidentalis (Hook.F) In Response to Plant Spacing and Liquid Cattle Manure. IOSR Journal of Agriculture and Veterinary Science, 8(12), 5-10. https://doi.org/10.9790/2380-081220510 Pandey, V., Chura, A., Pandey, H. K., & Nasim, M. (2015). Estimation of ascorbic acid, β carotene, total chlorophyll, phenolics and antioxidant activity of some European vegetables grown in mid hill conditions of western Himalaya. Journal on New Biological Reports, 4(3), 238-242. Retrieved from https://www.researchtrend.net Parađiković, N., Vinković, T., VinkovićVrček, I., Žuntar, I., Bojić, M., & Medić-Šarić, M. (2011). Effect of Natural Biostimulants on Yield and Nutritional Quality: An Example of Sweet Yellow Pepper (Capsicum annuum L.) Plants. Journal of the Science of Food and Agriculture, 91, 2146-2152. https://doi.org/10.1002/jsfa.4431 Pelesco, V. A., & Bentor Jr., M. A. (2013). Head Lettuce (Lactuca sativa L., Asteraceae) production in a non-circulating hydroponic system under the climatic condition of Biliran, Philippines: A preliminary investigation. Journal of Society and Technology, 3, 1-7. Retrieved from http://jst-online.org/index.php/JST/article/view/5 Poora, R. J. (2002). The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophyll a and b. Photosynthesis Research, 73, 149-156. https://doi.org/10.1023/A:1020470224740 Resh, H. M. (1995). Hydroponic food production. 5th ed. 23-26. Woodbridge Press Publication Company, Santa Barbara, CA. Resh, H. M., & Howard, M. (2012). Hydroponic Food Production: A Definitive Guidebook for the Advanced Home Gardener and the Commercial Hydroponic Grower. EUA, St. Bárbara. https://doi.org/10.1201/b12500 Rodriguez-Delfin, A. (2012). Advances of hydroponics in Latin America. Acta Horticulturae, 947, 23-32. https://doi.org/10.17660/ActaHortic.2012.947.1 Santos, J. A., & Ocampo, E. T. M. (2009). Principles of Hydroponics (with Emphasis on SNAP Hydroponics). Training Manual Version 1.3. pp. 1-17 Santos, P. J. A., & Ocampo, E. T. M. (2005). Snap hydroponics: Development and potential for urban vegetable production. Philippine Journal of Crop Science, 30(2), 3-11. Retrieved from https://www.ukdr.uplb.edu.ph/journal-article/4228 Shiyam, J. O., & Binang, W. B. (2013). Effect of poultry manure and plant population on productivity of fluted pumpkin (Telfairia occidentalis Hook F.) in Calabar, Nigeria Journal of Organic Systems, 8(2), 29-35. Retrieved from http://www.organic-systems.org/journal/82/8204.pdf Sumanta, N., Haque, C. I., Nishika, J., & Suprakash, R. (2014). Spectrophotometric analysis of chlorophylls and carotenoids from commonly grown fern species by using various extracting solvents. Research Journal of Chemical Sciences, 4(9), 63-69. https://doi.org/10.1055/s-0033-1340072 Treftz, C., & Omaye, S. T. (2015). Comparison between Hydroponic and Soil-Grown Raspberries (Rubus idaeus): Viability and Sensory Traits. Food and Nutrition Sciences, 6, 1533-1540. https://doi.org/10.4236/fns.2015.616158 Usman, M. (2015) Cow Dung, Goat and Poultry Manure and Their Effects on the Average Yields and Growth Parameters of Tomato Crop. Journal of Biology, Agriculture and Healthcare, 5(5), 7-10. Retrieved from https://core.ac.uk/download/pdf/234660759.pdf White, P. J., & Brown, P. H. (2010). Plant nutrition for sustainable development and global health. Annals of Botany, 105, 1073-1080. https://doi.org/10.1093/aob/mcq085 Publisher’s Note: ACSE stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. © 2022 by the authors. Licensee ACSE, USA. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). 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