AMERICAN INTERNATIONAL JOURNAL OF AGRICULTURAL STUDIES 6(1) (2022), 26-30 26 AGRICULTURAL STUDIES AIJAS VOL 6 NO 1 (2022) P-ISSN 2641-4155 E-ISSN 2641-418X Available online at www.acseusa.org Journal homepage: https://www.acseusa.org/journal/index.php/aijas Published by American Center of Science and Education, USA PRODUCTION OF WATERLEAF IN A SOIL AND SOILLESS SYSTEMS USING SINGLE AND DOUBLE NODE CUTTINGS Chukwunalu Okolie Ossai (a)1 Ayinde Mudashir Morakinyo (b ) Oyeyemi Azeez (c) Stephanie Clara Akpeji (d) (a) Research Associate, International Institute of Tropical Agriculture, Ibadan, Nigeria; E-mail: ossaichukwunalu1@gmail.com (b) Postgraduate Fellow, Crop Protection and Environmental Biology Department, University of Ibadan, Nigeria; E-mail: ayindemorakinyo@gmail.com (c) Seed Manager, International Institute of Tropical Agriculture, Ibadan, Nigeria; E-mail: o.azeez@cgiar.org (d) Lecturer, Microbiology Department, Novena University, Ogume, Delta State, Nigeria; E-mail: akpejistephanie@gmail.com A R T I C L E I N F O Article History: Received: 9th September 2022 Accepted: 20th October 2022 Online Publication: 29th October 2022 Keywords: Talinum Triangulare, Substrates, Soil Farming, Nodal Cuttings JEL Classification Codes: Q16 A B S T R A C T The general acceptability of waterleaf and its profitability as a vegetable has promoted its high demand in Nigeria. However, rural farmers depend on conventional farming systems for production with a low output. This calls for an alternative technique besides the conventional method to boost production and meet market demand. This study, therefore, evaluated the yield response of waterleaf cuttings to soil and soilless systems. Single and double nodal cuttings were cut from one-month-old waterleaf plants and planted in 4 kg cocopeat substrate and topsoil each, mixed with 250 g poultry manure each and arranged in a 2 (level of nodal cuttings) by 2 (substrate) factorial experiment laid in a completely randomized design with three replicates in 2 cycles. The plants were watered every other day. At harvest, data were collected on the Root Weight (RW), Shoot Weight (SW), and Leaf Weight (LW). Data collected were analyzed using ANOVA and differences in means were separated using least significance differences at 5% significance level. The substrate type was insignificant in the growth and yield parameters. However, the RW, SW and LW differed significantly between the nodal cuttings and ranged from 9.1±2.8 g to 21.2±2.8 g, 12.0±4.5 g to 27.0±4.5 g and 10.7±3.1 g to 21.9±3.1 g, respectively in the first cycle. The RW, SW and LW, also ranged from 10.1±1.8 g to 20.5±1.8 g, 12.7±3.0 g to 24.8±3.0 g, and 11.7±3.0 g to 23.7±3.0 g in the second cycle of production. © 2022 by the authors. Licensee ACSE, USA. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). INTRODUCTION Waterleaf (Talinum triangulare) is a leafy vegetable that originated from tropical Africa and grown in many countries including Asia, South America and West Africa (Tindall, 1983). Urban residents usually see it as a weed during the raining season because of its widening survival range. Like other leafy vegetables, it is widely cultivated in home garden to improve nutritional deficiency of less privilege family and mostly consumed in the southern part of Nigeria (Udoh et al., 2005). It plays a major role in eradicating malnutrition in Africa because it is a rich source of calcium, phosphorous, iron, protein and vitamins (Tata et al., 2016). The nutritional value and the affordability of the crop have made its demand higher among other common staple fibrous leafy vegetables. In southern Nigeria, it is used as herb for treating ailments and contagious diseases including measles and stomach upsets (Udoh et al., 2005). It performs well as fodder for raising giant snails (Ebenso & Okafor, 2002). Waterleaf is an important vegetable both as food and as raw materials for industries, which also serves for economic interest (Nya et al., 2010). In the past, the consumption of vegetable in Nigeria had been on the increase and currently estimated between 22 and 47.5 kg/person/year. The general acceptability of the vegetable by all classes of people and its profitability among other vegetables has promoted its cultivation by unemployment youths and the women of the rural population in the southern part of Nigeria. In West Africa, forecasters indicate that the urban population will reach 63% by 2050, enhancing the needs for effective urban and sub-urban agricultural production systems to complement rural systems. In addition, cultivation of this crop may provide additional income especially for female farmers. This therefore calls for a technique, beside conventional 1Corresponding author: ORCID ID: 0000-0002-1924-5855 © 2022 by the authors. Hosting by ACSE. Peer review under responsibility of American Center of Science and Education, USA. https://doi.org/10.46545/aijas.v6i1.265 To cite this article: Ossai, C. O. ., Morakinyo , A. M. ., Azeez , O. ., & Akpeji , S. C. . (2022). PRODUCTION OF WATERLEAF IN A SOIL AND SOILLESS SYSTEMS USING SINGLE AND DOUBLE NODE CUTTINGS. American International Journal of Agricultural Studies, 6(1). https://doi.org/10.46545/aijas.v6i1.265 https://doi.org/10.46545/aijas.v6i1.265 http://creativecommons.org/licenses/by/4.0/) http://creativecommons.org/licenses/by/4.0/) https://orcid.org/0000-0002-1924-5855 https://orcid.org/0000-0002-1545-6698 https://orcid.org/0000-0002-5697-4897 https://orcid.org/0000-0003-2191-8032 Ossai et al., American International Journal of Agricultural Studies 6(1) (2022), 26-30 27 method, which serves as alternative for massive production of waterleaf to meet the larger population as earlier projected. With the current security challenges in Nigeria coupled with urbanization, the call for the adoption of hydroponics system at home garden becomes increasingly relevant to meet the demand for vegetables in the country. This study therefore evaluated the morphological and yield response of waterleaf cuttings to soil and soilless systems. MATERIALS AND METHODS Experimental setup Buffered cocopeat substrate (5 kg block form) were purchased from Afri-Agri Company in Lagos State, Nigeria. The cocopeat was dissolved in 30 litres of water and poured into 12 hydroponics troughs of 8 litre capacity. Another twelve (12) troughs were filled with topsoil. Single and double node vine cuttings of one-month-old growing waterleaf plants were harvested. The vines were planted in the soil and soilless (cocopeat) troughs. Each substrate in the troughs were mixed thoroughly with 250 g poultry manure. The plants were watered three times a week for four (4) weeks before harvesting. The experiment was carried out twice. Experimental design The experiment was a 2 (number of nodes) by 2 (substrate) factorial laid in a completely randomized design and replicated three times. Data Collection and Statistical Analysis Bi-weekly, morphological data were taken on the plant height (cm), internode length (cm), number of leaves, and number of nodes, while at harvest, the number of new leaves, number of flowers and plant weight were collected. Data collected were analysed using analysis of variance (SAS 9.0 version) and differences in treatment means were separated using least significant differences at 5% level of significance. RESULTS Plant Height (cm) Table 1 shows that there were no significant differences between the substrates, nodal explants and the interaction between the substrates and nodal explants on the plant height in the first cycle of cultivation. However, at two weeks after planting, the height of waterleaf plants grown in the cocopeat substrate (22.67±1.37) cm was significantly taller than the ones grown in the soil (15.42±1.37) cm in the second growing circle. Number of Nodes On the number of nodes, there were no significant differences between the substrates, nodal explants and the interaction between the substrates and nodal explants on the nodal production by the plants in both first and second cycle of cultivation (Table 2). Table 1. Effect of substrates and explants nodal level in the height of waterleaf plants Substrates Plant height (cm) Cycle one Cycle two Two weeks Four weeks Two weeks Four weeks Cocopeat 22.70a 31.83a 22.67a 29.67a Topsoil 14.20a 28.17a 15.42b 27.33a LSD(0.05) 9.32 13.4 4.46 11.75 SE 2.86 4.11 1.37 3.6 SubxNodes 122.88ns 96.33ns 117.19* 16.33ns Double 19.12a 32.33a 19.25a 31.00a Single 17.78a 27.67a 18.33a 26.00a LSD(0.05) 9.32 13.4 4.46 11.75 SE 2.86 4.11 1.37 3.6 Means with the same alphabet down the groups are not significantly different from each other at 5% level of significance. LSD: Lead Significant Differences, SE: Standard Error, SubxNodes: Substrate by Nodes Interaction Table 2. Effect of substrates and explants nodal level in the number of nodes produced by waterleaf plants Substrates Number of nodes Cycle one Cycle two Two weeks Four weeks Two weeks Four weeks Cocopeat 13.00a 32.00a 17.17a 28.50a Topsoil 6.50a 24.50a 10.83a 21.67a LSD(0.05) 7.82 12.15 6.49 19 SE 2.39 4.02 1.99 5.83 SubxNodes 36.75ns 154.08ns 21.33ns 114.08ns Ossai et al., American International Journal of Agricultural Studies 6(1) (2022), 26-30 28 Double 12.00a 39.67a 17.83a 32.83a Single 7.50a 16.83a 10.17b 17.33a LSD(0.05) 7.82 12.15 6.49 19 SE 2.4 4.02 1.99 5.83 Means with the same alphabet down the groups are not significantly different from each other at 5% level of significance. LSD: Lead Significant Differences, SE: Standard Error, SubxNodes: Substrate by Nodes Interaction Number of Leaves There were no significant differences between the substrates, nodal explants and the interaction between the substrates and nodal explants on the number of leaves produced by the waterleaf plants in the first cycle of cultivation (Table 3). However, in the second production cycle, the plants grown in the cocopeat substrate produced significantly higher number of leaves (19.17±2.07) than the ones grown in topsoil (6.76±2.07). Internodes (cm) There were no significant differences between the substrates, nodal explants and the interaction between the substrates and nodal explants on the internodes of the waterleaf plants in both first and second cycle of cultivation (Table 4). Biomass (g) Table 5 showed that in both first and second growing cycles; the double node planting explants produced heavier roots (21.15±9.05) g, (20.48±1.76) g compared to the single node cut explants with root weights of 9.05±2.76 g and 10.07±1.76 g, respectively. The stem weight of the double node cut explants in the first (26.97±4.49) and second (24.82±2.96) g cycles were significantly heavier than the single node cut vines that produced 12.02±4.49 g in first cycle and 12.68±2.96 g, respectively. The leaf weight produced by the double node cut vines in first cycle (21.88±3.06) g and second cycle (23.72±2.98) g were significantly heavier than the double node cut vines in the first cycle (10.65±3.06) and second cycle (11.68±2.98) g, respectively. Table 3. Effect of substrates and explants nodal level in the number of leaves produced by waterleaf plants Substrates Number of leaves Cycle one Cycle two Two weeks Four weeks Two weeks Four weeks Cocopeat 16.33a 31.17a 19.17a 31.00a Topsoil 11.17a 26.67a 6.76b 25.00a LSD(0.05) 10.97 16.42 6.76 19.87 SE 3.36 5.25 2.07 6.09 SubxNodes 30.08ns 216.75ns 21.33* 147.00ns Double 16.50a 39.50a 19.33a 35.83a Single 11.00a 18.33a 11.67b 20.17a LSD(0.05) 10.97 16.42 6.76 19.87 SE 3.36 5.25 2.07 6.09 Means with the same alphabet down the groups are not significantly different from each other at 5% level of significance. LSD: Lead Significant Differences, SE: Standard Error, SubxNodes: Substrate by Nodes Interaction Table 4. Effect of substrates and explants nodal level in the internodes of waterleaf plants Substrates Internode length (cm) Cycle one Cycle two Two weeks Four weeks Two weeks Four weeks Cocopeat 2.87a 3.83a 1.90a 2.08a Topsoil 2.43a 2.90a 1.87a 2.05a LSD(0.05) 1.4 1.15 0.69 0.65 SE 0.43 0.35 0.21 0.2 SubxNodes 0.16ns 0.05ns 0.03ns 0.003ns Double 3.00a 3.37a 2.00a 2.13a Single 2.30a 3.37a 1.77a 2.00a LSD(0.05) 1.4 1.15 0.69 0.65 SE 0.43 0.35 0.21 0.2 Means with the same alphabet down the groups are not significantly different from each other at 5% level of significance. LSD: Lead Significant Differences, SE: Standard Error, SubxNodes: Substrate by Nodes Interaction Ossai et al., American International Journal of Agricultural Studies 6(1) (2022), 26-30 29 Table 5. Effect of substrates and explants nodal level in the biomass of waterleaf plants Substrates Root weight (g) Stem weight (g) Leaf weight (g) Cycle one Cycle two Cycle one Cycle two Cycle one Cycle two Cocopeat 16.58a 17.07a 20.98a 21.57a 17.08a 20.25a Topsoil 13.62a 13.48a 18.00a 15.93a 15.45a 15.15a LSD(0.05) 8.99 5.74 14.66 9.65 9.97 9.73 SE 2.76 1.76 4.49 2.96 3.06 2.98 SubxNodes 2.25ns 2.17ns 5.47ns 17.28ns 0.01ns 15.41ns Double 21.15a 20.48a 26.97a 24.82a 21.88a 23.72a Single 9.05b 10.07b 12.02b 12.68b 10.65b 11.68b LSD(0.05) 8.99 5.74 14.66 9.65 9.97 9.73 SE 2.76 1.76 4.49 2.96 3.06 2.98 Means with the same alphabet down the groups are not significantly different from each other at 5% level of significance. LSD: Lead Significant Differences, SE: Standard Error, SubxNodes: Substrate by Nodes Interaction DISCUSSIONS The findings of this study showed that the waterleaf plants grew at same rate (considering the agronomic parameters) in both the soil and soilless systems and the nodal explant levels in both cycles of production. This might be a result of the fertigation with poultry manure, which allowed the plants access to adequate nutrient for growth and development in both systems. Several research has established the usefulness of cocopeat substrate as a sufficient substrate for growing vegetables (Ahmad et al., 2011) as cocopeat substrate has high aeration capacity which allows easy root penetrations (Abad et al., 2002). Vine cutting technology has become a common system of multiplying vegetative propagated crops (Essilfie et al., 2016) of which waterleaf can be propagated through in addition to seeds. In the first two weeks of growth, the double node explants outgrew the single node cuttings in the second production cycle. However, at the fourth week of growth, the single node cuttings grew at same rate with the double node cuttings. This shows that the double node cuttings established faster than the single nodal cuttings, but considering propagation ratios, the single node cuttings is higher. The findings of this research supports the work of Dumbuya et al. (2017). The growth substrates had no effect on the biomass produced by the waterleaf plants in the two production cycles. Sarwar et al. (2018) had earlier reported the non-significance between topsoil and cocopeat in the production of vegetables. However, the double node cut vines produced heavier biomass in terms of root weight, stem weight and especially the leaf weight which is predominantly consumed by humans as vegetable soup and animals as folder. The double node cut explants benefitted from the early establishment in the accumulation of biomass compared to the single node cut explants. This supports the findings of Essilfie et al. (2016). CONCLUSIONS Both soil and soilless systems of cultivating waterleaf supported the production in the two cycles. However, there were no difference between the nodal explants on the agronomic parameters but the double node cuttings had an improved biomass production relative to the single node cut explants. Hence, waterleaf can be produced conveniently at ones convenience in the home garden to boost its availability. Author Contributions: Conceptualization, C.O.O. and A.M.M.; Methodology, C.O.O. and A.M.M; Software, C.O.O. and A.M.M; Validation, C.O.O. and A.M.M; Formal Analysis, C.O.O. and A.M.M.; Investigation, C.O.O. and A.M.M.; Resources, C.O.O. and A.M.M.; Data Curation, C.O.O. and A.M.M.; Writing – Original Draft Preparation, C.O.O., A.M.M., O.A. and S.C.A.; Writing – Review & Editing, C.O.O., A.M.M., O.A. and S.C.A.; Visualization, C.O.O., A.M.M., O.A. and S.C.A.; Supervision, C.O.O.; Project Administration, C.O.O.; Funding Acquisition, C.O.O., A.M.M., O.A. and S.C.A. Authors have read and agreed to the published version of the manuscript. Institutional Review Board Statement: Ethical review and approval were waived for this study, due to that the research does not deal with vulnerable groups or sensitive issues. Funding: The authors received no direct funding for this research. Acknowledgments: N/A. 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Udoh, J., Ndon, B. A., Asuquo, P. E., & Ndaeyo, N. U. (2005). Crop production techniques for the tropics concept publications limited. Munshin, Lagos Nigeria, 101-106. Publisher’s Note: ACSE stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. © 2022 by the authors. Licensee ACSE, USA. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). American International Journal of Agricultural Studies (P-ISSN 2641-4155 E-ISSN 2641-418X) by ACSE is licensed under a Creative Commons Attribution 4.0 International License. http://creativecommons.org/licenses/by/4.0/) http://ssbfnet.com/ojs/index.php/ijrbs http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/